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<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C4Y6</accession>
    <name>A0A8H6C4Y6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006806</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146903.1">
        <property type="nucleotide sequence ID" value="XM_037297702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C4Y6"/>
    <dbReference type="GeneID" id="59335206"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="28409" checksum="A52438CBF6626FF4" modified="2022-01-19" version="1">MSQRKSRAPSGSDDDSSGPRIPPVPQGGRGRRSGGDDFESPKRRRRHSGRGYDMPREKPLPSKVELQLCVFHGAPSHRWRTAPFTFDRRRDDDRELWEEIRRVYRDELQGVWRRILGFKKLKHIIPIEYTPNGVPIAKTAKDDPNSHSFMHAVHHPDRIRPAHEWVDWFTEFKAGDRGKPVGLEFQEGLWAEKLAIIAILITIAIIIVSIVWVVKGGQLQTVFTVMGFVLSGAAAEVALVALYFQVTTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6E9</accession>
    <name>A0A8H6C6E9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Isochorismatase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006819</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146916.1">
        <property type="nucleotide sequence ID" value="XM_037297715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6E9"/>
    <dbReference type="GeneID" id="59335219"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF15">
        <property type="entry name" value="BLR5631 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="4">
        <location>
            <begin position="25"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6217481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="190" mass="20270" checksum="CC45C5BF54265CEF" modified="2022-01-19" version="1">MAKQSGKSFRSFLGVEPSKPTTKDSVLVIVDAQNEYDHGLLAISDVKSSRAIIGDVLKKYRDANGDVVHVRHSTPDGAPLFTPKTELAEEFEELTQGAGASEKVVHKQHPSSFTGTDLHEHLDKLGKKKIAHVCISNTSRAGAELGYDVSVLADGIGDRDIPGASAKQLVDTTLAELGDVSATVIHSEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6H5</accession>
    <name>A0A8H6C6H5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147108.1">
        <property type="nucleotide sequence ID" value="XM_037297672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6H5"/>
    <dbReference type="GeneID" id="59335176"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="407" mass="44603" checksum="598656CFA22FAA74" modified="2022-01-19" version="1">MLFYEELGRHTPPPPPYSLSKRRTTKVKLENEASQPPPPPPPPPYLSSTPKPDCTIPSIDELWRQAVVSDGPYLARQGNAIPFPLVPLQRSATQVFDSEQCFGDSQGGLNCNWSCPGIPAHPVEPRWTPVGILGYPGPPLEGAMAPLPEYPLESGGHSVPRSFVFSPRTGIGLDHQKWTSQSPSTVSLGHNVVCHATQPPVHTLATRSMMDQKPLYTFRKQSSRDIASLANITTTDVTGSFGPSETMYSLSALPSQQAVEKRAKLTPRPTPQEFDAVRKVSDVKSPPDANQRSSSDGDASEGDGDSARVPKVAKGQDNAFPTRFKPDPRPSKDRTTSQRPDRVHHVSHSSHRRPSASARFEPYKRRQSKVAEMMKQRQGGAAEKGYAQSLQMQSDLDMNEGEGEETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6L9</accession>
    <name>A0A8H6C6L9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147400.1">
        <property type="nucleotide sequence ID" value="XM_037297275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6L9"/>
    <dbReference type="GeneID" id="59334778"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034617727" evidence="1">
        <location>
            <begin position="20"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="339" mass="37520" checksum="E282F0CCCFE9F62C" modified="2022-01-19" version="1">MKISFVCFLALSTLSFSRALTAATALTLGSDSSTPIAPTAGMSNSTLGIPIPPQSFGLTYEIGGPKLRITSCLVNTVAALKELALRDWDDKIIDGTEYELDDYPEVRIIITTPKRKRNVQVRFVLWAACLGVYDMILKRKFEFAQFEMSWERQVLGWVQVVNHPTSASLTTEKRQVNGTLDVGNISATLLSTDRTIGLEPISITNIVTMDNANDPAEARLNVTITPYGDTLGVYDVFVPIMSGLTDMAQFPSTHQVAGLIIGLEGFKGFICIVPAIPLRTSPPFMEYAWLIRAIARIPTYMLEKGRFGEVNIRIEVDGSLYQRAKELWIYEDENKGTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6U6</accession>
    <name>A0A8H6C6U6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006454</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="PTM">
        <text evidence="2">Carbamylation allows a single lysine to coordinate two divalent metal cations.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147477.1">
        <property type="nucleotide sequence ID" value="XM_037297352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6U6"/>
    <dbReference type="GeneID" id="59334855"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000174">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF96">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="8"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="64"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="347"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="103"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="222"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="387"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="473"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="2">
        <location>
            <position position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR611778-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="998" mass="112899" checksum="3C6A3225A8DF5DFE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C794</accession>
    <name>A0A8H6C794_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_006210</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147683.1">
        <property type="nucleotide sequence ID" value="XM_037297109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C794"/>
    <dbReference type="GeneID" id="59334611"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="38"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="289"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="385" mass="43016" checksum="C41DFE8DF1A07613" modified="2022-01-19" version="1">MSLLPVTLPPSELIQSRSAEVIGASVPLLILPTAAVALRLLSRWISRAGFWWDDLTIVLACILCWGPNIVDLVSLRYGFGRHVEVLGLGGSFRWFKILYSFEFLYTLAMASVKYSIILFQYRIFPIVQFRRILSWCTYFVVCLTISCVIVSVFQCVPIHAFWDTLAGTLAPELGGRCIDVELYFLISGSLNAATDFGLLALPIPILWRLRTSQPQKLILTGIFTVGLAVCAVSVTRLIMLHQYGKRYYVPIAVWTAAEPSIAVVSACLPSLRPLFVRVVWGGAHRPKPPTYPSTSRYGSNSLRKSPYGDGSFNRLQESDRSGNHGSPWTNHNVNVYGGRRSGDAESDEFELGNQAHFETPMNRIRAKTTVVLTISERVDFQDDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7N0</accession>
    <name>A0A8H6C7N0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Outer spore wall protein RRT8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005811</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147897.1">
        <property type="nucleotide sequence ID" value="XM_037296716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7N0"/>
    <dbReference type="GeneID" id="59334216"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059112">
        <property type="entry name" value="CysZ/EI24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF1">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN RRT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07264">
        <property type="entry name" value="EI24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="31816" checksum="CB89F050CD6E224A" modified="2022-01-19" version="1">MTESSKRSSTLRVITAPARAVSNASWLYPFKGIYYFCAHPFLWPLLRARLLPSALLSLFVLTNLFLWTYLPQVALLAVFHGHLAWLNATFLVLGEGAAIVSLLFEAFFVDETLVDVFDAVLVKQGYTDLIASSRILDPDGSDPVKQLGKPTTSAVYSPFSFRQIAEFIIFLPLNLIPVAGTPMFLIMTGYRAGPLQHWRYFKLLDFTKKERDAFIRKRRLRYTWFGTMALLLQLVPVLSMLFLLTTAAGSALWVAKLEGKRRALEAIPEDERSERYADDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7P4</accession>
    <name>A0A8H6C7P4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147604.1">
        <property type="nucleotide sequence ID" value="XM_037297030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7P4"/>
    <dbReference type="GeneID" id="59334532"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000138">
        <property type="entry name" value="NADPH dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="369" mass="40887" checksum="8DAAB320C2E58F32" modified="2022-01-19" version="1">MSPSLFDPIHIGSIPLSHRVAMAPLTRYRANDAHVHTSLATEYYGQRASVSGTLLITEATFISPRASGYANVPGIYNKDQIAAWKNVTDAVHKEGSYIFCQLWALGRTAKPEIVRKEAGEKWFASSSSVPMEAGTEEPRALDEEDIRGLVGDYVQAAKNAIEAGFDGVEIHGANGYLIDQFIQDVVNKRTDAWGGSVEKRSRFALEVAKAVVEAVGSEKVGIRLSPFSTFQGMKMAHPVPQFSHLVEGLKELKLGYLHLVESRISGNADVETTERVEPLLKIWGKTSPVLLAGGFQPDSAKRAVEKEYTGQDIMIVFGRHFISNPDLPFRIEKGIELTKYDRDTFYKTESPDGYVDYPFSKEWEAQSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7R9</accession>
    <name>A0A8H6C7R9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147957.1">
        <property type="nucleotide sequence ID" value="XM_037296776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7R9"/>
    <dbReference type="GeneID" id="59334276"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF13">
        <property type="entry name" value="OS03G0201400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="714" mass="82637" checksum="28E3EDEE312B00E8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7S5</accession>
    <name>A0A8H6C7S5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147860.1">
        <property type="nucleotide sequence ID" value="XM_037296679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7S5"/>
    <dbReference type="GeneID" id="59334179"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="134" mass="13981" checksum="BC15EA1E0D3318E4" modified="2022-01-19" version="1">MSKEYEGQDPMAIARQAERDLNSHQAKHGVSTADSTTESGVDTSVTDRFPGSTIKVGSAASGAGDNREIPVDEGGDLISKPGEKGGSGHPTKARDFEGPGGPETKQAIYEEANPGNDDVRNNVRQGTDTIRPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9D6</accession>
    <name>A0A8H6C9D6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148446.1">
        <property type="nucleotide sequence ID" value="XM_037296464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9D6"/>
    <dbReference type="GeneID" id="59333961"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34213">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2 (NTF2) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34213:SF2">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2 (NTF2) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="194" mass="21851" checksum="29264C101FDCAC76" modified="2022-01-19" version="1">MTSRAETIQQNHELRPDPQSLTSNPDASQNSSTGVDYEPHPEQSIKVSPEREKIVKSITSLYSGSASEEDMQVYAKEAIYDDPWSYCDTRYKIAGQWYGIPKVMASSKTLSTEIVSSTDTELIFKMKQEYTPRVVQSTKPVNSLVSLTLDGEGKVKYHKDMWNDKDYSHEGLGKIMKTLNGDHLTKITRPPGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA45</accession>
    <name>A0A8H6CA45_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149157.1">
        <property type="nucleotide sequence ID" value="XM_037294469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA45"/>
    <dbReference type="GeneID" id="59331958"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="290" mass="32714" checksum="5CBFB08616519E13" modified="2022-01-19" version="1">MRYSWNEKVKFKWEQVYSNTIDLMADLAVLGWNSELRTFPVTVFAGRRFTTQIVVDTLLPPLQIKYLVWGLYQTGLQIGRGKHARLQADMVLQGRPLGFFTYDTRRYETKLPGLSGYPTNSTLLLNGDNPVNYTSIAASSQKSDLQLSPSSILTVDHGTAFDPDDTKLKIYYEFDGVKISSSQIFTAYLDALATAAVHGHDETDAAIIAYSEDRHTSINVRRDISCTSFTWGILIRALFTVWEYGTLGYATLRDKRWEGLSFTIDYDGKPAGEGLILYNPRPPHELATSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAE5</accession>
    <name>A0A8H6CAE5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP-binding protein ypt1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003712</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149322.1">
        <property type="nucleotide sequence ID" value="XM_037294634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAE5"/>
    <dbReference type="GeneID" id="59332123"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18102" checksum="1510B4BC1F47BA89" modified="2022-01-19" version="1">MADRLYQKIRTIELDGKTVKLQIWDTAGQERFRTITSSYYRGAHGICVVYDVTDMDSFNNVKQWLQEIDRYATEGVNKLLVGNKSDMSDKKVVEYTVAKEFADSLGIPFLETSAKNASNVEQAFLTMARQIKERMGTTTVNNKPTVQVGQGQGVQSGSAGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAE6</accession>
    <name>A0A8H6CAE6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003692</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149302.1">
        <property type="nucleotide sequence ID" value="XM_037294614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAE6"/>
    <dbReference type="GeneID" id="59332103"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000085">
        <property type="entry name" value="Putative ras-related protein rab-11a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="211" mass="23546" checksum="5F55DA71C990DF90" modified="2022-01-19" version="1">MANDEYDFLFKVVLIGDSGVGKSNLLSRFTRNEFNLDSKSTIGVEFATRSIQVDAKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDISKHQTYENVQRWLKELRDHADANIVIMLVGNKSDLRHLRAVPTEEAKQFASDNNLSFIETSALDASNVELAFQNILTEIYRIVSSKALDNGDSAQNPIGERKVLEISKSSDPETKSGKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB58</accession>
    <name>A0A8H6CB58_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitrogen regulatory protein areA GATA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149660.1">
        <property type="nucleotide sequence ID" value="XM_037294279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB58"/>
    <dbReference type="GeneID" id="59331768"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052292">
        <property type="entry name" value="Glucose_repression_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051:SF1">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="587" mass="65301" checksum="E9E49169AC38A2FA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBH6</accession>
    <name>A0A8H6CBH6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149827.1">
        <property type="nucleotide sequence ID" value="XM_037294446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBH6"/>
    <dbReference type="GeneID" id="59331935"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="235" mass="25630" checksum="B7CC8AAC1FB2784C" modified="2022-01-19" version="1">MSSPNSASAAIPPSSTHPSSSVRVPLTLDELNRYVCQEAFNRAELPVIPPTFIGPHPPPTSSTFIGTKYLVGGPTNETPVRIMFRCVSPHDKVSRHVLMKMKPSVPEEALALKLCEVFEIPATGGVISKTEPEFVPGLSPLRVPNKVYSYGEKFEWLGASMAGFVEVTVVPVEQMNVCTNCGIRKTVNAVTLQNIRKWCFGVNSLCIKCCRTGAVPGNVTELCMPDGQIFSRLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBH9</accession>
    <name>A0A8H6CBH9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149810.1">
        <property type="nucleotide sequence ID" value="XM_037294429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBH9"/>
    <dbReference type="GeneID" id="59331918"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="213" mass="24877" checksum="60D4188E969474B0" modified="2022-01-19" version="1">MSRVAALCVGDHPDPNFKREGPFRYFDLPTEVRDQILRLLLVRENVALGREKSIRNRFSGWSHEKPMWQLLRVNGQLRAEAKVVLFSQRNNTFYFPIGGTLTGVAWWYMLGRPSPHIQAVPPPIRKLDCAFDMRDFEETTFNMFEGTKRDYHDEMAPGDVPFERLPPIERLDLIHDSYRSNMLRVWRGIAEAFCMMKLYLLRLDVSKARAADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC02</accession>
    <name>A0A8H6CC02_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150205.1">
        <property type="nucleotide sequence ID" value="XM_037293376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC02"/>
    <dbReference type="GeneID" id="59330863"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="2">
        <location>
            <begin position="63"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="219" mass="23735" checksum="5BFC0A328AA40DEC" modified="2022-01-19" version="1">MAIMMSLHGVSKHQWDVPRSEIPFIVRAVTPAAIYDVCILAYTILMFLNIFLYVKPVVDTNKALGAVNLFSDIYILCAPIAAVSKFQLSIKAKFGIILVFMIGTIACAKSLVGLIFRLQVHQTEASVGALTNGYGAHAVVCMAGMAAHKQAMRLMLNKGLCIVGSSVGTPQHMDELLALAVAGKVLPLVEVIEFDDLNHALRRLARHEVEGRIVVNTPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC52</accession>
    <name>A0A8H6CC52_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149974.1">
        <property type="nucleotide sequence ID" value="XM_037293896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC52"/>
    <dbReference type="GeneID" id="59331384"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="150" mass="16580" checksum="CEF0BEAFE7C68948" modified="2022-01-19" version="1">MSSNSQIIRGFTAEQNHSFTNILLPALQAFDKHFNPLQPQQKQSVIMPKPAQQEQEQKHKPLQQANVEKSTEKSSDNKVTIIKNTAQLSKIPRHTLSIIPHQATMHNCGDPSPRLSSTPLSTSLLASLLTLASPLGYIRYAKDMEAIGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC59</accession>
    <name>A0A8H6CC59_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002553</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150308.1">
        <property type="nucleotide sequence ID" value="XM_037293479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC59"/>
    <dbReference type="GeneID" id="59330966"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="185" mass="21287" checksum="CC54BCB48597B2FB" modified="2022-01-19" version="1">MTAPTHVKVLVLTQKGLQYPEYIKSVDTPNRQRIPSTDLDPKLRALLKSQIPTITSHLYHGTEHADYSDVGPLPDTRLHYWSENAWAKRTVICLSKYHVFCTLQKGETLLPNPWSGNIFSGDMFILKFPNTEGPDGRRFYEDIDPGTTLREILRVDAEVAAVFEEAMKEYFTRRWEVSATVESWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC63</accession>
    <name>A0A8H6CC63_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149931.1">
        <property type="nucleotide sequence ID" value="XM_037293853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC63"/>
    <dbReference type="GeneID" id="59331341"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="40427" checksum="4121F5087118385D" modified="2022-01-19" version="1">MSSPLSEVDRELLGEISYNNGTKATMAEANGGPGLGTHEYSHGAEFTPLNATAGTLVKVSSNGDELQADEGTNMDNTKPAPKTPKKRGAAASANDDSAQPTKKRSPTKKATAATNAETNINGEASDPITPSSKKRASPKKKSAADPNVETVDGETIEETEGPEPVTPKKGKRVTAKGKATKADNDVKIDNESAETTDKSLSPIKGNGESNATTVHNMPRKRQAPKKELAAPRGIPSSWDNADHADRMMVTMKEKGEGWAEIRAAWKEATGQDTASSTLPNRYNRIKVNMMRMKEGDDQHLLLAKKEIEDAYNTGFWANVAANMERRGADKYPSAFLQKTFKELEAAGKATINPVNGTAATGAEENDAIKEEDTGEGDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC99</accession>
    <name>A0A8H6CC99_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7924 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150006.1">
        <property type="nucleotide sequence ID" value="XM_037293928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC99"/>
    <dbReference type="GeneID" id="59331416"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057684">
        <property type="entry name" value="DUF7924"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25545">
        <property type="entry name" value="DUF7924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7924" evidence="2">
        <location>
            <begin position="45"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="226" mass="25802" checksum="85F159AA36E7D423" modified="2022-01-19" version="1">MVKKTKNRDAEGEMMIMMESYAANDGLRSQEKPNLATGFVPGKVDTVKALGMTEPNPDTVFGKLRPQFIKPGTRGPSDIKAIKSLGCIVDWPFFIFEAKPAQTAIPHARNQAQRDCGAVLKSLVKLKAYVEGRGYKKKTGAVEAFWVFSLCWNPEYANILVHWVEIMDDETEIFHVTKLRQKFMDDEEGRAALRAYRHNVFDHGSSHMSPPWRRYGRRPSRRETLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCD5</accession>
    <name>A0A8H6CCD5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002659</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150413.1">
        <property type="nucleotide sequence ID" value="XM_037293584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCD5"/>
    <dbReference type="GeneID" id="59331071"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1040:FF:000003">
        <property type="entry name" value="N-terminal acetyltransferase A, auxiliary subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1010:FF:000002">
        <property type="entry name" value="N-terminal acetyltransferase catalytic subunit (NAT1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF2">
        <property type="entry name" value="N(ALPHA)-ACETYLTRANSFERASE 15_16, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="587"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="610"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="843" mass="96059" checksum="BD8B8E797ECBD575" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCF0</accession>
    <name>A0A8H6CCF0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150124.1">
        <property type="nucleotide sequence ID" value="XM_037294046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCF0"/>
    <dbReference type="GeneID" id="59331534"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="811" mass="89212" checksum="9F5EE8A777EFFA83" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCS5</accession>
    <name>A0A8H6CCS5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150356.1">
        <property type="nucleotide sequence ID" value="XM_037293527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCS5"/>
    <dbReference type="GeneID" id="59331014"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13577">
        <property type="entry name" value="SnoaL_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="9"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="154" mass="16722" checksum="5BC80864B00B82DF" modified="2022-01-19" version="1">MAYPSSLASLQVREAISDALYRCVLGLDTANKHLFDSAFTHDASFDLNGTVMSGLDAITTGCYDSISKLDTTHFMSNVRVDVGAGTPTASMTASALAQHYRSKQGTEPGAPRLLTGSLYSLDLLEDDKDGLWKIKHWKLHLVWAEGDWGILTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD02</accession>
    <name>A0A8H6CD02_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-hydroxyacid dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002655</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150409.1">
        <property type="nucleotide sequence ID" value="XM_037293580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD02"/>
    <dbReference type="GeneID" id="59331067"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12168">
        <property type="entry name" value="Mand_dh_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000282">
        <property type="entry name" value="Glyoxylate reductase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF269">
        <property type="entry name" value="HYPOTHETICAL D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="5">
        <location>
            <begin position="68"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="6">
        <location>
            <begin position="124"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="36827" checksum="DF4DC00ED8D7BD64" modified="2022-01-19" version="1">MSKPKVLLLGDIEHPSAKESFDSLSSIAQLVTPNSDNPSDFLKECRSGAFNGVRAAYRTFNSVKITGRIEGEVCEELGKAGLRFLAHNGAGYDQCNIPDCTKAGIHVSNVPTAVDAATADTSLFLMLGALRMFASPLINLRKGEFRGKTPPPLGHDPEGKLLGIVGMGGIGRDFKKKAEALGMNVQYHNRSKLSDEMSGGAKYVGFEELLEKSDVISLNVPLNKSTHHMISTPQFSKMKDGVVMVNTARGAVIDEAALVKALSDGKVFSAGLDVYEEEPKIHQGLVDNPHVLLLPHMGTWTYETQEKMEHFVVDNVRSALETGKLKSPVHEQKDMPLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD16</accession>
    <name>A0A8H6CD16_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002774</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150528.1">
        <property type="nucleotide sequence ID" value="XM_037293699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD16"/>
    <dbReference type="GeneID" id="59331186"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034396093" evidence="3">
        <location>
            <begin position="23"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="400" mass="43982" checksum="C1C11823C6E98B5C" modified="2022-01-19" version="1">MLFTNLTVWFASSLSLIASAQSVWSAATPVTKFPILPRTANQSTTGVSPASGTKKYFAETFCTPYQKLIEKIAWRDALQYAQSLASWQPNGSFQPAMDLYMGNDSRGQLGATLRANIDGAVRVHDQSTWPRNQRLFVFCSEPMIFVENGGLCGELGAVAYAFNINGTYYDNHYVVFCPPYYELQTLYKTLSITDAFPPERLLVTSANDFDENQGEVYFHESLHFADLTTPVNHPIHDNNGNESDIYGPYDVAMEAESKNTAACLHIADAYTNAAAAILIQQRYNLADPPKPRKSVFQRNLGSQADGHNDTFERLPQLPNPNTPDNHIVSNDFGIGPVASSSHQLIPGSSSLSKRVVWPPKFPCTAENRCVRSMAATIATTISGPLICFLVAGLCVLSFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD39</accession>
    <name>A0A8H6CD39_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Transfer RNA methyltransferase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TRM8</name>
        <name evidence="11" type="ORF">HO133_002752</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with TRM82.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD39"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="114"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="151"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="283"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="313" mass="35261" checksum="4289EF2EB0FD6949" modified="2022-01-19" version="1">MTAAGKRQKREDYRKTHKEEGNAELPKKKFYRQRAHANPFSDHRLTYPASPALMDWSSHYPAFVAPDTQSVAKDHAVVKPLTKPVTVADIGCGFGGLLVALSPLLPDELILGLEIRTQVTEYVEDRISALRSQYADRSPRLYQNISCLRANTMKFLPNFFTRGQLSHIFLCFPDPHFKARKHKARIVSGTLCAEYAYVLRPGGCVYTITDVEELGEWMRSRFEAFGNSGVGGEDEGGETGEGRTEGMFETIELPEEGKEGEWVDEGGQKGLVAMLVRCIREETEEGKKVTRNGGKRFVSVFRRREDPAWPDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDK0</accession>
    <name>A0A8H6CDK0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150942.1">
        <property type="nucleotide sequence ID" value="XM_037293289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDK0"/>
    <dbReference type="GeneID" id="59330776"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="465" mass="53399" checksum="10F74DB07000B850" modified="2022-01-19" version="1">MLSFCLHRRSLSILIFPFIIVTALIITQTGLHHSIPGPLGRTKQPIPTVKPTPLHKPISTATPTPVIDAFPLAASAKSPADLPPIPSWNNPPTPHVNESTPLFIGFTRNWPLLQQTVVSYITAGWPPEDIYVIENTGVMHSNRNNLLTLQNPFYLDHHRLTKILSVNVISTPTLFTFAQLQNFYTFTALENNWSHYWWAHMDTVVLSDEEWDGESYKSLYMRAVDALRETLDPAWGPLATRWFAYDKLALVRTQAFVEIGGWDTMIPFYMTDCDMHERLWMREFRIEGAEAGRVWDVHTSLDDLERLYKRGADSPKATAKREGATEILPREPEGQRNSAVYQELVHSLNLLQDLKSNAEGGRNTWQARQRGGHGEPFYRDPEGFEKGIWMWMDFGRDVYTEKWGRGPCNLREAGLVEEDAWRLVKDWENADVQKQFRLDREKEAKEQVIKEKAKEKEGAKEKTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDM5</accession>
    <name>A0A8H6CDM5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="8">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_002159</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for PP2A-like phosphatases modulating their activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2Ai) in presence of ATP and Mg(2+) by dissociating the inactive form from the complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150739.1">
        <property type="nucleotide sequence ID" value="XM_037293086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDM5"/>
    <dbReference type="GeneID" id="59330573"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0697">Rotamase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="425" mass="47350" checksum="1118BBED1F2CB04D" modified="2022-01-19" version="1">MPSTIGTEHVPNPISTDGQRKLPRLIPRKKAPSQNALPVPGTPNLPPPSDSSILIFQEPTRRILSSHDHGIFLSSPTYSLILSFVFSLSESATDKKISSVDVPTTHPSIHKILSILDSIEETITRCPPGDQGGSRFGNPAFRDFIDSISSQSQSWHQRLGLHSQSSIEEASIYLYHSFGNRQRIDYGSGHELNFVMWLLCLNRLSLLPTSTFSALTLAVFPRYIKTLRRLQSTYYLEPAGSHGVWGLDDYHFLPFLFGASQLLHHPYIRPLSIHNEIMLEEEGGEYLYLDMVRFTMASKTVKGLKWTQPMLDDISAAKNWEKVEQGMRRMFVKEVLGKLPVMQHFLFGSLVPAEGMSREEDAGVAPESEEKEGGELTVGENGVGHVHRDDAWGDCCGIKIPSTVAAAQEAKKRGEGDGLRRVPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDQ8</accession>
    <name>A0A8H6CDQ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase</fullName>
            <ecNumber evidence="6">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002241</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150821.1">
        <property type="nucleotide sequence ID" value="XM_037293168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDQ8"/>
    <dbReference type="GeneID" id="59330655"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF404">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034543168" description="Carboxypeptidase" evidence="6">
        <location>
            <begin position="32"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="648"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="720" mass="77166" checksum="4E117C324E33D0EC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDY5</accession>
    <name>A0A8H6CDY5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exocyst complex component Sec6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002266</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150846.1">
        <property type="nucleotide sequence ID" value="XM_037293193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDY5"/>
    <dbReference type="GeneID" id="59330680"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000006">
        <property type="entry name" value="Exocyst complex component sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.70:FF:000005">
        <property type="entry name" value="Exocyst complex component Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="107"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="748" mass="87123" checksum="FBEBE0F7579F9B71" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE00</accession>
    <name>A0A8H6CE00_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">OPT family small oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001633</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151100.1">
        <property type="nucleotide sequence ID" value="XM_037292561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE00"/>
    <dbReference type="GeneID" id="59330047"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="526"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="559"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="731"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="758"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="840"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="908"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="950"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="993"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="680"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="693"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1053" mass="117258" checksum="38E19A553714D2E2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE85</accession>
    <name>A0A8H6CE85_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151054.1">
        <property type="nucleotide sequence ID" value="XM_037292515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE85"/>
    <dbReference type="GeneID" id="59330001"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="99" mass="9996" checksum="17031BA784B85981" modified="2022-01-19" version="1">MSSTGASDSGSNPSMLHGHAAYVAAAAKETIGSYTSADMHSAGSADKQAAVDEMRAAKQASDAQGEEISKNQTLGKVEQSLGSATGCEGMEEEGGKRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFK2</accession>
    <name>A0A8H6CFK2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial-processing peptidase subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Alpha-MPP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Inactive zinc metalloprotease alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Matrix processing peptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_001412</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151761.1">
        <property type="nucleotide sequence ID" value="XM_037292342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFK2"/>
    <dbReference type="GeneID" id="59329828"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000023">
        <property type="entry name" value="Mitochondrial processing peptidase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000032">
        <property type="entry name" value="Mitochondrial processing peptidase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF49">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="12">
        <location>
            <begin position="56"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="13">
        <location>
            <begin position="209"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="13">
        <location>
            <begin position="364"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="250"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6222326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="586" mass="63792" checksum="49D0C821033D5211" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG75</accession>
    <name>A0A8H6CG75_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">37S ribosomal protein Rsm22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001039</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152334.1">
        <property type="nucleotide sequence ID" value="XM_037291972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG75"/>
    <dbReference type="GeneID" id="59329457"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="753"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="440"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="764"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6222988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="780" mass="87228" checksum="FE224C2D5DC7796D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGP3</accession>
    <name>A0A8H6CGP3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152462.1">
        <property type="nucleotide sequence ID" value="XM_037291027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGP3"/>
    <dbReference type="GeneID" id="59328506"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="352" mass="39646" checksum="BDDEE379C0B6169C" modified="2022-01-19" version="1">MDSPSAYGTLSAFPVEIRRQVWHELLCEPVAILTPLWVLRSRDGLYYVKIPERTPVDVAIFRSSQACGVEAMEVFYSEHTFSFCALPSLWELECMDINYSLRVAQFITDFRTRMPSYPTQLAAHHIRDVRMSISIPRSNGCFGSYQTSDHSLCIALMHKLQASGKVRKTCSIDLLFFGIDKCPELIEVLGPTFFRAFKTLTAFNKVTINSSGFPRPAYLTFNPDKSYASCLDCYKNTALICYVMQRIVEELEPALGPATVLDGSYTHIAEFRPRIRPAESGKAGTILAVQTNMGNRGIGLKSLIERLGRHQAMRTSEWGLFPCGRRAMSDGVFTIWSYSKERIAAAISRIMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHQ6</accession>
    <name>A0A8H6CHQ6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000596</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152970.1">
        <property type="nucleotide sequence ID" value="XM_037291535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHQ6"/>
    <dbReference type="GeneID" id="59329015"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22997">
        <property type="entry name" value="GT_LH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36587">
        <property type="entry name" value="EXPRESSION SITE-ASSOCIATED GENE 3 (ESAG3)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36587:SF2">
        <property type="entry name" value="EXPRESSION SITE-ASSOCIATED GENE 3 (ESAG3)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="572" mass="65189" checksum="520B6AD969EC872F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI38</accession>
    <name>A0A8H6CI38_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152878.1">
        <property type="nucleotide sequence ID" value="XM_037291443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI38"/>
    <dbReference type="GeneID" id="59328923"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="283" mass="30935" checksum="C07CBFF008B33029" modified="2022-01-19" version="1">MTSEQNKQLPKLAPKAVIGVKRKPNRKDLYDRRDPHDQKPLRGLPDPDPDVVLRRAALNLEQAALRMQHAIPEANPESSLRESLTVLADPEAPWSDSDWETITDPDSGSELDDEGEKGVEDAFIAKVRGVEVGEVTAVRVAPVRTNIPAFIKKVRAVKGRGSNVAAAAVPVLPKTRMQPGDTRELTAAEAALALTNRQAGTSVTMEDLRPTKKTKVVRADGDKYCWTVGSVSTRALTVAEAALVLTNVQAGTNVTMQDLRTAREMVDLWDNQDTFEWTVGNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIK8</accession>
    <name>A0A8H6CIK8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010823</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153308.1">
        <property type="nucleotide sequence ID" value="XM_037301674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIK8"/>
    <dbReference type="GeneID" id="59339213"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36578:SF1">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36578">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034758760" evidence="2">
        <location>
            <begin position="17"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="348" mass="37197" checksum="1E3773A6BCEAA200" modified="2022-01-19" version="1">MYIPFILAALAALTSALPSPQLIDLVAIEEYPDAVRVTAPIDIVKDVVPSVPAAPIRPLTSIISSSKRDLTVEKRDGDCSPYPTGSGPVPSPDTVDAFESFPTFAAMATAAPTPDGYEVVFINSNASLTASDYMGLYTLTTYDTLGCASQCDQASGCQAFNMYIERDPSLNASADNCPNPPSTTNYKCTLWGAPVSVEEATNSGQYQDSFQVVIAGSNAYNKDAPPPACDGYNGPTELGGAINAPPSSGSYMGYQYFPFSQSQGYDPQTCADACNAQTTYDSENLAADGSYMACIFFNAYVLSEDGIPQGLYCSMYNETWDASYGTNYGQYQGDDRYTVSRSYSYALM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIY1</accession>
    <name>A0A8H6CIY1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010990</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153473.1">
        <property type="nucleotide sequence ID" value="XM_037301839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIY1"/>
    <dbReference type="GeneID" id="59339380"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="116"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="272" mass="29726" checksum="F69C4D1250889F8A" modified="2022-01-19" version="1">MSPQSPIDQDIKGDGEQLRTSTSIYPITEEGDQDDSQQPEHNMDGPSLRRPDTPIAGQQPEITDSTPCESTEPSSPANASSATPTSGTSTNSPLGRPPPSSKHYYLEEMWPGQPICHLCRKEYEKTNRAIRALPCGDIFHRACIDHLLGQKDGRCPSCESPIPPEWYMPHKFDPSKPPGSPCLCHPASKARTTTEPNTKNQGPSFTSPGVLAIRKVYQRIKEIMVRCAPGRKPEKVYASYKTESGQTRYIEGETGVGLPGPGSGYADGDLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJL9</accession>
    <name>A0A8H6CJL9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_010971</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153454.1">
        <property type="nucleotide sequence ID" value="XM_037301820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJL9"/>
    <dbReference type="GeneID" id="59339361"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="112"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6224394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="285" mass="31503" checksum="6A5FF17DE5E36A9C" modified="2022-01-19" version="1">MLYLIGLGLADETDVTVKGLELIKKAERVYLEAYTSILLVDIANLEAYYGRPIIVADREMVESASDEILAGADTSDVAFLVVGDPFGATTHTDLVLRARELQIPVRSVHNASIMSAIGATGLQLYNFGQTVSMVFFTDTWKPSSFYDRLKENQQIGLHSLLLLDIKVKEQSLANLARGRKIYEPPRFMTVAECASQMLETEEERGEGVCGPQNLAVGVARVGAWNQKMAAGTLKQLSGIDLGAPLHSLVLLGKKTHYLERDFVRDFAVKQDVFDTVWSELYEGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJM4</accession>
    <name>A0A8H6CJM4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH-ubiquinone oxidoreductase 29.9 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009952</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I NDUFA5 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153625.1">
        <property type="nucleotide sequence ID" value="XM_037300808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJM4"/>
    <dbReference type="GeneID" id="59338344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006806">
        <property type="entry name" value="NDUFA5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04716">
        <property type="entry name" value="ETC_C1_NDUFA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26681" checksum="1F0AD244B5B50656" modified="2022-01-19" version="1">MRNTIRLLASVKSGRYLEAGNPTGLTGLFTHPAPRSTLLYLYGATLDKLKAFPDHSVYRQSVEALTRHRMQIIESIKPEGYDEWAKKAAEKIEKFPEAFQPGANYNYKSAGGQHFVTVEDKGSEDQEWTSLEGTRTSEEKAAEVALLRKGRRQDYSKVVNWEPEPPLEASQISEAENQIGGGLIEEVIQVAEGELKLVDTMAESKVWEDIEEKPPQGQWDYFARDQHTSGTQEPPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJM6</accession>
    <name>A0A8H6CJM6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aldose 1-epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_011018</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153501.1">
        <property type="nucleotide sequence ID" value="XM_037301867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJM6"/>
    <dbReference type="GeneID" id="59339408"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000014">
        <property type="entry name" value="Aldose 1-epimerase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF6">
        <property type="entry name" value="1-EPIMERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G13240)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034954351" description="Aldose 1-epimerase" evidence="4">
        <location>
            <begin position="18"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="42158" checksum="0188736BD6433593" modified="2022-01-19" version="1">MIFPLLALSALSLGALAQNSSGSSVFTKYTISAPGINASFIPYGARLTNLLVPDKNGKTQDVILGYDSGTTYLNDTETDHTYFGAVVGRYANRIKNGTFTVQGVTSHIPENEHGGEDTLHGGNVGYDQRNWTVVSSNTSSITFSLLDQGYEGFPGTVITYATYTVSSGPTWTSRLVSIPLDEPTPIMLANHVYWNLDAFSNPSDLTILNDTLHMPYSARYVQIDNIEVPNGTIGIVNGSSLDFTSPKQIGRDILNAIDDCGFNCTGYDNAFILDRPRYSAPEATDISVLTMYSNVTGIQLDVYTNQQSLQLYACDNLNGTIPVKMSQQHGNSTSYVPKFGCLVIETQQWIDGINNPQWGQDQYQIYTPTTQPAVNFAQYDFSIVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK67</accession>
    <name>A0A8H6CK67_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Polyadenylation factor subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010254</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for 3'-end cleavage and polyadenylation of pre-mRNAs. Also involved in chromosome segregation where it has a role in chromosome attachment to the mitotic spindle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153926.1">
        <property type="nucleotide sequence ID" value="XM_037301109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK67"/>
    <dbReference type="GeneID" id="59338646"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002008">
        <property type="entry name" value="Polyadenylation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="179"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="221"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="263"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="378"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="432"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="525"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="448"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00026154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="620" mass="67610" checksum="0AD95EB5E85C5A77" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKR1</accession>
    <name>A0A8H6CKR1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154130.1">
        <property type="nucleotide sequence ID" value="XM_037301313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKR1"/>
    <dbReference type="GeneID" id="59338851"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="880" mass="100345" checksum="06F00D31614C0534" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLR5</accession>
    <name>A0A8H6CLR5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PQ loop repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009551</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154260.1">
        <property type="nucleotide sequence ID" value="XM_037300412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLR5"/>
    <dbReference type="GeneID" id="59337946"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF37">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="43711" checksum="DE17FA78DE1C5D57" modified="2022-01-19" version="1">MAPQTSIPVAANILGTIGTVFWCIQLVPQIWYNWKQKKTDGLPGLMMFLWAISAVPFGVYAIVQNFNMPIQIQPQVFCALALVSWGQVLIYNKLLVQHPSLTKAVSSKWTTRKASVVGTVVGVAFGGIEALLILTIRPLYDRQVAWPIMFVGIVAAILLAVGFLPPYFELWKRSGRVVGINFIFLTIDWLGAFFSLMGVVAQNTFDPLGGCLFIVCIVIEGGIFVSQGIWLFRTRKLRAAAKRAGRDLDELPQSDVYHVEVPRKGSIAASRDVERDEIARRGSLAVARDLEHGEGSMLSVLQQLEDGISKESRSKTDVEVRCEEVRAGSLEGVQEIDYGTMERSSAASRTSGSRKHERSRMLRLSQIPPFHYAASGIANIHRWLLCTPFAVWMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMG0</accession>
    <name>A0A8H6CMG0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008984</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154671.1">
        <property type="nucleotide sequence ID" value="XM_037299845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMG0"/>
    <dbReference type="GeneID" id="59337379"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034198424" evidence="1">
        <location>
            <begin position="20"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="464" mass="50337" checksum="84EC05D8438CCBF4" modified="2022-01-19" version="1">MALFQLFLLVASFSHLITASPPKVNTTAYPASAVITRDICIIGGGSTGTYSAIRLSDSGKTVVVVEAKDRLGGHTNTYTDPSTNETVDYGVVYFHNLEVVKEYFARFDIPLTVAPSTVPGQVTEYVDFSTGKIVAGYAPPDPSAALAAYGAQLEKYPYVEAGFDLPYPVPADLLLPFGDFVKKYNLENAVGFLFSFAQGLGNLLDQYTLYVFKNFGIGVLQALQTGFLTTARRDNSEIYEKARAELGSDVLLQSHVIATDRSGDDGVKVLVQTPQGVKLILAKKLLITIPPKPDNLKGFDLSTNESSLFAQFLNSAYYTALLRNTNIPSNTTLTNIGPSTPYNLPSLPGLYSIYPSRVSGLYDVKVGSAFALPDAQVQALIVESVLRLRTAGTLNATAPEFAAYASHTPFELTVSEEAIRGGFYRELYALQGQRHTWYTGAAFHTHDSSLLWRFTEGLIPGILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMJ3</accession>
    <name>A0A8H6CMJ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009003</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154690.1">
        <property type="nucleotide sequence ID" value="XM_037299864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMJ3"/>
    <dbReference type="GeneID" id="59337398"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="9"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="293" mass="34660" checksum="1399430FCBDF95B4" modified="2022-01-19" version="1">MAPPAHVDDYYDILNISNTAAAGEVIASYRRLVKIRHPDKKRGSTDAFQQLGKAYEILKDPVERRAYDSVWPGIRDRLGTQHQSDLRQAEAAEAETRREAEARAKKQEEARKRRGAEARAKTKEEDEARRSRGAETRAKDLENAGRQKSFNLGEYDKDISDLHQHTTTLEKDLNQLEDQDNEDPRIEKEQNSYRTYLSSYFYPKAKETEEQKQARENERIQRRTSRRFKEILLTTTKGKLQRQRDALQRVIDQIAAEEEPVEYSKKKEESGGKWRRKTRTERRRMRRRRRKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNJ1</accession>
    <name>A0A8H6CNJ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PurM-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008391</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155258.1">
        <property type="nucleotide sequence ID" value="XM_037299257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNJ1"/>
    <dbReference type="GeneID" id="59336787"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055181">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22689">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like C-terminal" evidence="1">
        <location>
            <begin position="26"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="FGAR-AT PurM N-terminal-like" evidence="2">
        <location>
            <begin position="130"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="1">
        <location>
            <begin position="289"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="650" mass="69674" checksum="A9E9566DFB117B4D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPP4</accession>
    <name>A0A8H6CPP4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">GPI inositol-deacylase</fullName>
            <ecNumber evidence="5">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008701</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GPI inositol-deacylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155566.1">
        <property type="nucleotide sequence ID" value="XM_037299565.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337096"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054471">
        <property type="entry name" value="GPIID_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF16">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22939">
        <property type="entry name" value="WHD_GPIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="6">
        <location>
            <begin position="33"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="8">
        <location>
            <begin position="317"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase winged helix" evidence="7">
        <location>
            <begin position="582"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1177"/>
            <end position="1218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6227258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1557" mass="173528" checksum="8E6A8F205D132388" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQR7</accession>
    <name>A0A8H6CQR7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155797.1">
        <property type="nucleotide sequence ID" value="XM_037298461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQR7"/>
    <dbReference type="GeneID" id="59335988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="298" mass="32606" checksum="470E7C28302BBD6F" modified="2022-01-19" version="1">MSAQASGGAGGDDKKLDKASRVRRLWGFDQGWSNQIPGHFGQGYGSFPTPSMGQGTVGSFGNVSLPTSSYGGHKQGTAKWHEMQKQIRGPRDNRKPRCKEHSKDKKEEKGPLAVKDASIEKPISKSARQRANKRASQKKKEEEKKKEEEEPAQDPLTGPADLELVSLWDESRFENAGNVENAETVEAAPLTDERMESVAPSPEDIVIEVGNIDAQSLIDEVFETPSGFQFPQDQTRVGIMAGLLEQFGQERMLDLSESAAMNGTTIVWEAFQQDLFKEQIAVVVAGMPLSNSGINKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR53</accession>
    <name>A0A8H6CR53_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glutamate decarboxylase</fullName>
            <ecNumber evidence="3 9">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_008090</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3 9"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156294.1">
        <property type="nucleotide sequence ID" value="XM_037298958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR53"/>
    <dbReference type="GeneID" id="59336487"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000017">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.50:FF:000001">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321:SF3">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="474"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6228360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="501" mass="56285" checksum="146EFEDD3960BBF6" modified="2022-01-19" version="1">MTPYSSRYASQQEISKFKIPKEGAPADAVFQMLKDELDLDGRPNLNLASFVGTYMEREAEALMVENLSKNMSDADEYPAMMVSIISHLWGVQKGETAIGSATTGSSEAIHLGGLAMKRRWQEKRDAEGKDKTKPNIIMGSNAQVALEKFARYFEVEARILDVSEKSKYRLDPELVKKNIDENTIGVFVIMGSTYTGHYEPVEEISKILDEYEEKTGNDIPIHVDAASGGFIAPFTHAHAGGPKWNFALPRVKSINTSGHKFGLVYAGVGWIIWRDESYLPRHLIFELHYLGGTEESYTLNFSRPGAQIIAQYYNLIHLGFDGFRAIMENCLSNARLLSKSLEATGWYTCVSDIHRKNKDGFNEENETSADYIAGLPVVAFRLSDAFKKDYSHVKQESISTLLRVKQYIIPNYPLPPKEEKTEILRVVVRESMSLDLLDRLITDIIAVTEQLMNADPMDLSAFQPGATTIEKQHQSKGVDHVNKHKAHRPMSKGVHLSEMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRA9</accession>
    <name>A0A8H6CRA9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007861</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156067.1">
        <property type="nucleotide sequence ID" value="XM_037298731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRA9"/>
    <dbReference type="GeneID" id="59336258"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22439">
        <property type="entry name" value="KH-I_PCBP_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22455">
        <property type="entry name" value="KH-I_Rnc1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22456">
        <property type="entry name" value="KH-I_Rnc1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="141"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="224"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="423"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="331"/>
            <end position="420"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
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            <begin position="110"/>
            <end position="131"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="391"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="499" mass="52361" checksum="8FB74BCBA1C01ADF" modified="2022-01-19" version="1">MSASPSTLAATPQANQKRPLDDESSPSGPTDQPDAKRPALDKAVKEDEGTDEDAKTSTTLANEDAPLASTEGVKAESGAAPVANGHTDTQGDTVVPDAPNGAGPSAASVLETQPIQSTGGQYDRATSQGPMQHQGHQDETGWLHIRAVISSAEAATVIGKGGENVSQIRRFSGAKCTVSDYSRGAVERVLTVSGLVDATAKAFGLIIRTLNNEPLEAPSTSQSKTYPLRLLVPHILIGSIIGKSGVRIREIQEASGARLNASDSCLPLSTERSLVVLGVADAVHIATYYVGQTLVEQLTERFGGPAASAYATRSGGPAGVVPGGMQVVPYVPQPAGGQYGHPDNYRRHNNQPQRTPANPYGVPYLHGQPPQQQPQAPVHYGGSPQQGYAGAGPHQPPQHGAHQPTHSHAGPPAQPVQGMVPGQPLTQQIFIPNDMVGAIIGKGGAKINEIRQLSGSVIKINEPQDNSNERLVTITGTSECNQMALYMLYSRLESEKHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRG3</accession>
    <name>A0A8H6CRG3_9LECA</name>
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        <recommendedName>
            <fullName evidence="5">MARVEL domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ORF">HO133_007927</name>
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        <name type="scientific">Letharia lupina</name>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>Lecanorales</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6228197.1"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
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            <begin position="96"/>
            <end position="117"/>
        </location>
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            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
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            <begin position="188"/>
            <end position="281"/>
        </location>
    </feature>
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            <begin position="236"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
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            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="31476" checksum="9D1DD848F1061C47" modified="2022-01-19" version="1">MFPRIELPRDRVSKKPSPYPTLAFHLLRLGQLLSSIIVSSVLLFFIHHLHKEEYYIPWTFILLLTVSLLTIIALMATTILHFTLTLNPKYNLLSNLSLLILWVLGLSLLSWNLGWTLGHRCTSVTWHNDSGIMVCRLYKALTAFTVTGLVSTLLALALDLRVQKKSTQLGSYNQMLDIKGPTDLRSSSPFHGGAEGFEPAHRRESSPYHTGALEATYDDQRRHPSPCESEGIETAYSHRRDPSHDAARPYKVQKPIEAEQFGYSAPSEQTSYGGGGGTGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRQ5</accession>
    <name>A0A8H6CRQ5_9LECA</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">HO133_008175</name>
    </gene>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>Lecanoromycetidae</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228445.1"/>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
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        <location>
            <begin position="117"/>
            <end position="160"/>
        </location>
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            <begin position="12"/>
            <end position="23"/>
        </location>
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            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6228445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="160" mass="17606" checksum="113D863C4AE96249" modified="2022-01-19" version="1">MTVMGVSAACEGLHKHEDKAPKSDDDDENIVEDAERFMVIEDSAVEEEDTELDAASFGLAVLTTHLALVCDAPVNSRFTVSAKLSYDGRFSQKTRQSAQALRPLPARTRAEKFALTGDLGSSLREDQKARKSNQPIIDPHKAGLEAKAHEKSQAYRYQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRS6</accession>
    <name>A0A8H6CRS6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pleckstrin homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">HO133_008102</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156306.1">
        <property type="nucleotide sequence ID" value="XM_037298970.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336499"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032065">
        <property type="term" value="P:maintenance of protein location in cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053005">
        <property type="entry name" value="Nuclear_Pos-Cytoskel_Interact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024774">
        <property type="entry name" value="PH_dom-Mcp5-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190:SF2">
        <property type="entry name" value="MIGRATION PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G07730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12814">
        <property type="entry name" value="Mcp5_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pleckstrin homology" evidence="3">
        <location>
            <begin position="935"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="387"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="552"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="743"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="861"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1173"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="557"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="602"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="649"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="743"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="876"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1202"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1228"/>
            <end position="1245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1323" mass="145504" checksum="D627820332B6A83B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRX1</accession>
    <name>A0A8H6CRX1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fimbrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008178</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
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    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="term" value="P:formin-nucleated actin cable organization"/>
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        <location>
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            <end position="51"/>
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        <location>
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        <location>
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</entry>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
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    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6228241.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="614" mass="68982" checksum="FB00F9AFE2B49312" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSE4</accession>
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        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
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    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
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        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
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    </dbReference>
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        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
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        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
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        <property type="entry name" value="J_dom_sf"/>
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        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034471519" evidence="7">
        <location>
            <begin position="19"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="21"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="145"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="145"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6228376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="412" mass="46602" checksum="78C7CAB12785ABCA" modified="2022-01-19" version="1">MMFLPALILGFLLPLVLCVEDFYKLLDIGKSASDREIKKAYRLLSKKYHPDKNPGNESASQKFVQIAEAYDALSEPETRRIYDQYGYEGLKQHQQGGQGGRSHDPFDLFSRFFGGSGHFGHESGQRRGPDMEVRISVPLSDFYNGHQHEFSIEKQQICEECEGSGSADGTVETCGQCGGRGMVIQKHMLAPGIFQQMQMGCDKCGGKGKTIKHPCKVCGGSRVVKKLASHTLHIEKGMPKGSRIVYENEADESPDWVAGDLVVNVGEKEAEYGDEGQRTDGAFFRRKGRDMFWKEVLSLREAWMGDWERNLTHLDGHVVRLRRKRGEVIQPNQVEVVAGEGMPVWLQEQEQSRAGEEEYGQLHVEYTVVLPDQVEKPMEKEFWALWEKWRKKIGVNLAKDSGRPPPTEHSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSJ1</accession>
    <name>A0A8H6CSJ1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156455.1">
        <property type="nucleotide sequence ID" value="XM_037299119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSJ1"/>
    <dbReference type="GeneID" id="59336648"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="308" mass="34658" checksum="16E094ECE0D4857B" modified="2022-01-19" version="1">MSSTTAKTPQGDPKHGGALFRLPREIRDVIYRRLVKGRYLTDKRSAHSEGSDKEGSDSESDVDQNAGPNLSILRVSKAVGHEAVEILYSDSVFRFPIDFKSPRDDDEDGKLFHLNHLKKVTPMMKNVYLDVDGASIRWAGWFETRYSPMWARKLELSFGPTIDLFGDVDIKRRTLHIRMSECCARMLEGTSFGTICQRLKSLVGFSVATVEVMLSRDVPQAPACFGFNNAEQTRRARDLMRYITHKITKELEPAFGPAISGLQLDAGNMPIQKRNFSEGGDTTLVGFLEFHPGKHSVQECTAEEERLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7K7</accession>
    <name>A0A8H6F7K7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147662.1">
        <property type="nucleotide sequence ID" value="XM_037297088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7K7"/>
    <dbReference type="GeneID" id="59334590"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="190" mass="21579" checksum="0AB02982CBFF1E36" modified="2022-01-19" version="1">MVARMTLGGVVAKPYVSEHSLFSRYSRDQRVEDNVHLATIGFEPVKKSDEALFLWKSYLGAHNLAPVNAVSTGLSNTLRHWQQFYPFPTTSTVHCYKGPFSGSIAFNELESHLENPEDVALLFNSIPKNHERSDELASQVILGFAIAKREIPTKDWRAQEVARNRQVKPGEEALPFRKRLLAKLRRKYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8B8</accession>
    <name>A0A8H6F8B8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005551</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148442.1">
        <property type="nucleotide sequence ID" value="XM_037296460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8B8"/>
    <dbReference type="GeneID" id="59333957"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF305">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="468" mass="49584" checksum="A993C63D778C7769" modified="2022-01-19" version="1">MSKPQHITFTEPSTPVEANHSARRTLKDRDNILHASQYADSTAPEGGYGWAVVFACFSISFWIVGTVYSWGVMQAALFKQGLSSPPTLSWIGSLDFACIAFLALVNARVIRLLGARGTALLGISMLALGEILSGFLTHNVGGLFVTAGVVSGIGTSLCFMVINVTPAQYFHKKRGLANGIVCAGGGLGGTVISLSMNAIIQRVGPAWTFRILGLLTLATGLPAAWLIKERCPIRSATFIEWKLFRDFKFVTIFLVGVVATFPLLVPPFFIPLYSASLGLSSSTGAALVAGFNISSGVGRLSSGLAADALGPLNTLFIGLFLTTVSMFVIWPVSDSLGPLVVFVIINGAANGAFFATMPTVVGTVFGSQRVSVAMGMVVTGWAGGYLMVRRAYLPPQSEERLESDRAMLIGSLQGAPIAGYLLEAHGGEKSTLAAYHPAMFWAGSLAVLAMGLVAMVRLYVNKDIMRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8C6</accession>
    <name>A0A8H6F8C6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005857</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147943.1">
        <property type="nucleotide sequence ID" value="XM_037296762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8C6"/>
    <dbReference type="GeneID" id="59334262"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF168">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="23063" checksum="39AC36120FE4F6AE" modified="2022-01-19" version="1">MVAGYAGKENLRLESEIDERIHDLISLIERKRMDMAQKAQFFTLDVISGWAFDQAFGDLIVDEDVFEYIKTVDKAMHVLLSMSELPDVYSFPEKSSLMKLVAPSATDRIGFGKLIAIAKEKVAERFGDNRKVKVLWRLKAEIDEAVDNGSISSPTQDSEARPLLYLQACMKEGLRLWPPVTGLFPKVVPPEGETSNGVFLPGGIEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9D0</accession>
    <name>A0A8H6F9D0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_003641</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149251.1">
        <property type="nucleotide sequence ID" value="XM_037294563.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332052"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000223">
        <property type="entry name" value="Major facilitator superfamily domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF31">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="14"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="1474"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="1744"/>
            <end position="1766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="462"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="512"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="579"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="678"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="780"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="982"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1020"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1074"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1239"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1948"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="470"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="525"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="536"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="631"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="791"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="806"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1032"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1109"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1960"/>
            <end position="1972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2041" mass="223057" checksum="F75023AFAF284C01" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9S1</accession>
    <name>A0A8H6F9S1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149972.1">
        <property type="nucleotide sequence ID" value="XM_037293894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9S1"/>
    <dbReference type="GeneID" id="59331382"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF56">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="2">
        <location>
            <begin position="144"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="489" mass="54384" checksum="F80A6858D9B7285A" modified="2022-01-19" version="1">MAFDQTENTEIPLIDSLQPAIDSSHGTSGGVSDSPTRSVNASPTRSSNSNGSVRTSSTAAYERAEREAEDAFNQRIENLCQDLWPSPTSIKHRFLASQAALRLRTNKFFRSFVPAPQVPLIEHLKGGGFNHITSITLPSSYDEGHRNLILRVPREGESRPDQQVATLDYVRQRTAIPVASIESTDFTCNNAVGKPYAIQHRIPGRDLDSVWDVLSRSQRCVVAKEVGCLIRALLSVESPVAGTIEAATNGSYTSAELPNIVPFILTDARGDLVEELEPDATSGAGTARSSETTVEFFEYYLSRWRKSALAESLGENNCDVELYDDMLKVVHEMNALCLFKPELNCLCHLDLHPRNIMVEINSEDSVRVTGILDWDEAVVAPKFVNCQPPGWLWGYDKDTHTENSLLPWPYELEGANNTPSTLEQQELKSIFDDHAGPEYPRLAYSESSRLMRGLYRVATLGLTANWHSAAAERIVKEWNILRPNLTHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9U9</accession>
    <name>A0A8H6F9U9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lysophospholipase</fullName>
            <ecNumber evidence="6">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003060</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the lysophospholipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150062.1">
        <property type="nucleotide sequence ID" value="XM_037293984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9U9"/>
    <dbReference type="GeneID" id="59331472"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00147">
        <property type="entry name" value="cPLA2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF40">
        <property type="entry name" value="PATATIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLA2c" evidence="8">
        <location>
            <begin position="154"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="29"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="597"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="609"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="770" mass="86696" checksum="E199D3FEA94F4958" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA51</accession>
    <name>A0A8H6FA51_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003470</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149773.1">
        <property type="nucleotide sequence ID" value="XM_037294392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA51"/>
    <dbReference type="GeneID" id="59331881"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000019">
        <property type="entry name" value="RNA exonuclease NEF-sp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF115">
        <property type="entry name" value="FI18136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="8">
        <location>
            <begin position="344"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="721" mass="79687" checksum="69DA5ACB30F4E7BA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAD4</accession>
    <name>A0A8H6FAD4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150540.1">
        <property type="nucleotide sequence ID" value="XM_037293711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAD4"/>
    <dbReference type="GeneID" id="59331198"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="144" mass="16144" checksum="8D7817D3E8C6AFEC" modified="2022-01-19" version="1">MSPTIVDLTVASPAVTRVSEERQEETARFESREVSCVYRKNKTEDDADQDQEEDEDEEVGTDTRDDEDDSEDSDDANGEDDGGDKEEEEDRPHIFSNGAPLIHADGVKRLRSRSATCTNCSEEFDVTDNGKDSCVWHPGEREPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB95</accession>
    <name>A0A8H6FB95_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001120</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151470.1">
        <property type="nucleotide sequence ID" value="XM_037292051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB95"/>
    <dbReference type="GeneID" id="59329537"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="102"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="336" mass="38106" checksum="1EE2A9BA4AA8F906" modified="2022-01-19" version="1">MKAAQGKMAQERQPKKLRSGRSSGSKRRKAKNNANQLARTTPASDSQAEPRIRRKTAAQQEGRNARDARRLAAVSGVAILSKVPPGQLPDQGNWEEDERKTYPCSICGRKFVKKNHVKVHMAACVKRNGNPNGARWDDAWRNRASQAASMDTQSNTTSAPQTLHIHDFQAEDDQTEDKQPEDYQFEHHQREDFERPDVSFGGFQRDHCIFDHLPHEPASTSSPGMQGYDVELLTESSQSDDLDFIDLQSASSSPSDLTPYDPDLRMDVLTNETLLLMAQAWQKAIEYVGETHTVVHPHFQRIMEELGRRGMIDENWTYVVDLVRVNMTWRRVTLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBA4</accession>
    <name>A0A8H6FBA4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002335</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150914.1">
        <property type="nucleotide sequence ID" value="XM_037293261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBA4"/>
    <dbReference type="GeneID" id="59330748"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07992">
        <property type="entry name" value="LPLAT_AAK14816-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052744">
        <property type="entry name" value="GPAT/DAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605:SF0">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="481"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="515"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="3">
        <location>
            <begin position="48"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="701"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="667"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="703"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="756" mass="82735" checksum="6691AFB3F274F9D0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBL8</accession>
    <name>A0A8H6FBL8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001851</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151318.1">
        <property type="nucleotide sequence ID" value="XM_037292779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBL8"/>
    <dbReference type="GeneID" id="59330265"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="490" mass="53838" checksum="BA0B526C310155F8" modified="2022-01-19" version="1">MESGLWTCQALWASAIYDILQWEQPLGLKHNLCSSTHHSTDSKARADNGNLLNSVSLKKFLDQEAHRHINTDSDSEILPNYFASELNESGKARVNAEDCFKSLERTYKKCEGSWACTAMIAGLGLIGFRDPYGIRPIVIGSRTVEGGTDYMIASESVALQFLGCRLEDIRDPLPGEAVIIVKGSEPVFRQVQPKANYTPDIFEVGRLFAHGISVENARFKMGEKLAATIKKELGPERLAQVDVVIPIPETSNTSACAVAKHLKKELASGFVKNRYIFRTFLMSSQRLRRSGVRRKLHAMSAEFKDKTVLLVDDSIVRGNTSLEICLMAREAGAKSIIFPSASPPVRHSHIYGVDLASADELIAYSKTPAEIAVAIGADTVIYQTLPDLEAACAELSPRGIDCYFESGVFSGCYVTGVPEGHFEHLERVRGRNKKPKILEAARRTIGDTSTDEDLSQAVAGVELGSTQSPIASYSQDISLHDFNDYALPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD33</accession>
    <name>A0A8H6FD33_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RAD52 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010636</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAD52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153122.1">
        <property type="nucleotide sequence ID" value="XM_037301488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD33"/>
    <dbReference type="GeneID" id="59339026"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045002">
        <property type="term" value="P:double-strand break repair via single-strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.80:FF:000001">
        <property type="entry name" value="DNA repair protein RAD52 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.80">
        <property type="entry name" value="DNA repair protein Rad52/59/22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004585">
        <property type="entry name" value="DNA_recomb/repair_Rad52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041247">
        <property type="entry name" value="Rad52_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007232">
        <property type="entry name" value="Rad52_Rad59_Rad22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042525">
        <property type="entry name" value="Rad52_Rad59_Rad22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00607">
        <property type="entry name" value="rad52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RAD52, RAD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04098">
        <property type="entry name" value="Rad52_Rad22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="200"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="436"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00077224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="545" mass="58827" checksum="617FC59AF64B33C4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDR8</accession>
    <name>A0A8H6FDR8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153406.1">
        <property type="nucleotide sequence ID" value="XM_037301772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDR8"/>
    <dbReference type="GeneID" id="59339313"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="89" mass="8417" checksum="BB1ED4B6A169B427" modified="2022-01-19" version="1">MPRGGEYDDGPTVASDNAIESGENKIAGAPEGDSDEPVSSGVDRSGKAAPLPEGISEMKDAPGSGGGSQGVTPGGGEKTTGGGSGKGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE33</accession>
    <name>A0A8H6FE33_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010293</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153964.1">
        <property type="nucleotide sequence ID" value="XM_037301147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE33"/>
    <dbReference type="GeneID" id="59338684"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF191">
        <property type="entry name" value="VACUOLAR BASIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="439"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="55"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="547" mass="58469" checksum="A576E5331781608E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE38</accession>
    <name>A0A8H6FE38_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010308</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153979.1">
        <property type="nucleotide sequence ID" value="XM_037301162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE38"/>
    <dbReference type="GeneID" id="59338699"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="155" mass="17251" checksum="7F77B22216D93816" modified="2022-01-19" version="1">MTNPYANIEYVMANVVPIQNSIMSNINRGDLTAMYDAGIPTPISATVQRRNLQRARCSEDLLPPMTSARTCPNTSLDPLRMNECEGYWRDHPPRPEGLLPPLRQPDGYWPPLTLCDPHDGPHHGRLPHPGMPGRDEPDRVGRGRVVQDAQSGVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEH6</accession>
    <name>A0A8H6FEH6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009532</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154241.1">
        <property type="nucleotide sequence ID" value="XM_037300393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEH6"/>
    <dbReference type="GeneID" id="59337927"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF15">
        <property type="entry name" value="HOMOSERINE ACETYLTRANSFERASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_1G15350)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="66"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000443-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="334" mass="36781" checksum="1C4DDDC52163D3C6" modified="2022-01-19" version="1">MPSSTYDATGVEYYEIPSFTFESGASIPIKVAYRSYNPSSSKVVCIPTCYGGKINTTLTFNEENGALSSHHVVLVAMLGNGESSSPSNTPNFPKTLDYRDCIHAQYELLSKHLGVSELDAVIGFSMGGQQAYYWASMYPTFVKNAVVICGSARTSGHNYAFLEGPKTALLNSADYADGAYRAQGIRPLRGLRAFGRAYCAWLTSGAWFRERQWEKSLGFRSVEEYIANVMEKSFEEWDAEDVLILARMWQAGDVGTTRDDGSYEKALEGIEARVLVMPGRTDQYFAVADSEVEVGLLRKGELAVIESVWGHAAGGGMSEADTKWMDGRIREFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEN9</accession>
    <name>A0A8H6FEN9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010259</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153931.1">
        <property type="nucleotide sequence ID" value="XM_037301114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEN9"/>
    <dbReference type="GeneID" id="59338651"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034987504" evidence="2">
        <location>
            <begin position="22"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="458" mass="49281" checksum="805324B2C714DB0B" modified="2022-01-19" version="1">MHTSRFSRLAIAIATADVAAAAVLMTTPVNPPLPLQPRDLDLVQPSALEPTPNQTKLTGFDLVQAGTLARPSTNATIGQWVVANPPEDIANQSSLLLQKCVGNIDNVDVSAPNVSDWSCSLPANLVARDGPGRRLPYEVENVRPLAHLRTPQYNTLYTKTWSVTYEDADGNGYNITFPAGLCPDNVIGDIESPYRGEMVYNATLLSLYQSLIGLSPERLYNYTMSMLEAELANLYAALDAIICDPPIGGSRALLQRYRPQDMTGKITLILVGRVGGFAMVFSALVSAIAHEGLTVHVPAIGNVLLVAAVGAIAAAFERFINRRRQRGDVQVMEAYIANAFVAAAAQIRLHAIAIWSMTCTRSEIILENIKKLLAGNADGIPQAVARPDGNADDVAIVPQNPSLYPEQGRLEQEGSILTTKSKGKPSQNLIQESTNGGGANLTYLLRTDANVCKEQNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF91</accession>
    <name>A0A8H6FF91_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009193</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154880.1">
        <property type="nucleotide sequence ID" value="XM_037300054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF91"/>
    <dbReference type="GeneID" id="59337588"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1230.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001931">
        <property type="entry name" value="Ribosomal_eS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018279">
        <property type="entry name" value="Ribosomal_eS21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038579">
        <property type="entry name" value="Ribosomal_eS21_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10442">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01249">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002148">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00996">
        <property type="entry name" value="RIBOSOMAL_S21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="88" mass="9731" checksum="81003F616F0FBB6E" modified="2022-01-19" version="1">MENDKGELVDLYVPRKCSATNRIIKAKDHASVQISVGKVDENGRYTGENQVYALCGFVRAMGEGDDSLNRLAQRDGLVKNVWTGSSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFD8</accession>
    <name>A0A8H6FFD8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155015.1">
        <property type="nucleotide sequence ID" value="XM_037300189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFD8"/>
    <dbReference type="GeneID" id="59337723"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF33">
        <property type="entry name" value="PROTEIN TMA23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="33218" checksum="BC451E794E29DC64" modified="2022-01-19" version="1">MDAAAYLTNQGWRGDGHALHHSGRGITKPIHISQKANVLGVGKKQHDAHADQWWARAFDDTLKGLNNTKNEATGKTEGIAFGSGAQALQMVGVGGAKWVGQGGLYSNFVRGESLNGTLTPEENDHRETQSQSEDQRKQERGSNDVDLTAAAVKDVKRSKRKRRHQEAEDIAEFSKLVAVDAVQQDPKDVGREEEYQKLRERPKDTETNEQRRQRKREKKARRALEAGQSRELPEQPWSTASNDISTSDRPKRRSKEQLHKAEDAEPVATRPRNASQDVKRKGKSGKRRRAADTQCTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFF3</accession>
    <name>A0A8H6FFF3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ML-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009221</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154908.1">
        <property type="nucleotide sequence ID" value="XM_037300082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFF3"/>
    <dbReference type="GeneID" id="59337616"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF6">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="478"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="559"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="588"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="9">
        <location>
            <begin position="74"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="788"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="937"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="858"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="996"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1009"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1074"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1123"/>
            <end position="1132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6226355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1232" mass="133132" checksum="2B571F7B56BC5DCC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFS2</accession>
    <name>A0A8H6FFS2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transglutaminase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008338</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase-like superfamily. PNGase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155206.1">
        <property type="nucleotide sequence ID" value="XM_037299205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFS2"/>
    <dbReference type="GeneID" id="59336735"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.620.30:FF:000004">
        <property type="entry name" value="Peptidase (PNG1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050883">
        <property type="entry name" value="PNGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143">
        <property type="entry name" value="PEPTIDE N-GLYCANASE PNGASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143:SF19">
        <property type="entry name" value="PEPTIDE-N(4)-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="5">
        <location>
            <begin position="150"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="308"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="357" mass="40547" checksum="261CAD27C36574D7" modified="2022-01-19" version="1">MASPPQDPASLKFRNLLITLSVNPTKYENPGLLDEALAVIPLDRIYSEAEDESQLLQAEAASTGPNAKPQWGYQDCVIRALLKWFKRSFFQFVNNPPCTVCGSPTLANGFAPPTPDEAARGAAKVESYRCSSTDCNALERFPRYGDVWALLQSRRGRCGEWANCFSMLCRAVGGRVRWVWNAEDHVWTEVYSEQQRRWIHVDACEEAWDNPRLYSEGWGKKMSYCIAFSIDGATDVTRRYVRNAASHGVERNRCPEEVLMFITNEIKRMRRETMLKDERKRLLIEDEREERELRGFVVQALAAEIGKMLPTGRNGGGEEIKVPEQAARRSGAQAWREARGENGGDDTGRDRPPREDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGP6</accession>
    <name>A0A8H6FGP6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155774.1">
        <property type="nucleotide sequence ID" value="XM_037299773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGP6"/>
    <dbReference type="GeneID" id="59337305"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="130" mass="13860" checksum="1CF65B26EC5EFB78" modified="2022-01-19" version="1">MPRTPWTSGVITKIAPDRSSVVIRACAGGRRARFGDAGTIERLGLDTGARKRVRFTRKRGGRATTTRRREVGEVEGDFERGVRKRGRLMGGEGGVMGHLGVVLRVSWVCGLVISAACIGVGDLELGAGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGY5</accession>
    <name>A0A8H6FGY5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155757.1">
        <property type="nucleotide sequence ID" value="XM_037299756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGY5"/>
    <dbReference type="GeneID" id="59337288"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="390" mass="44652" checksum="8533FBBAA405ECAA" modified="2022-01-19" version="1">MIASKVYRKFDLLETYPASDNEGSGLDSTRIRSTASILMTSSKDQATSSTKPKPKHKHVHENGKGLSNGCPWIPWPQWREGEPKTKNYKTPDIGWTEFSRKNRDGTSYEYLNTILQRPGTPMNRSLVKTPLRQILPTVLKGVKGHPTMLKHSLTVLWRLGQIQSNLDYNGAAVFINITGAGARDDTAFFRLYMGQDDILELVHNIQEKLGTLLFQTLPTKTLHQFEIEPKFPNIHCNVLSHLLQSQGNRWEAKYIEEAMKFLQETDDPEILQHYQEHAGQDPLKTLIIERALEKRGGVAHTFETLPESIKNYLSPRGFTDRNEVEAIIARGATLVLSILMIMSSLGNATKHALNDHEHIKQYRLQETYENHHSDDYVKSRQRYQARIERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH01</accession>
    <name>A0A8H6FH01_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007962</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156166.1">
        <property type="nucleotide sequence ID" value="XM_037298830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH01"/>
    <dbReference type="GeneID" id="59336359"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="417" mass="48628" checksum="A6C2E03D989EB21B" modified="2022-01-19" version="1">MKYLPDMAALSRLFTAYPRTFIIFNLISKEIFAGIVDNMSAELRNSALDVLAVRSHPPIHPSQITDFIKHHLDAKVCYTQPRLRRYPLSAILDLITISESIESLTESFASDRVLGPCIRHSMPLSPIELHRIRRSFWRFQLCYDMCHPEEVSSSREICEVKSRGTRQYVQSQSKQPISGSVPNWLQGRSEAYRPEALSRFLPNLSRWEHDELEAVRFHLALKVNRLQYLRSCGSKDELIREPALLQRLIRDIDHWDTGSPEDHVLVAAFRQSQHARSHPIVWHRIRHCYDASIPNTPRRFVIQSLQVGCPQWGWCLWDEKRLVKRGMIDIEYEGWLDGWKMDDESKIAKICERRAAIGEAHSECIAAQYTLLDQKIAAQYAIDAQIHLDRLRWDRVWELGSKERKWMPTELDCIYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIA4</accession>
    <name>A0A8H6FIA4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007141</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156669.1">
        <property type="nucleotide sequence ID" value="XM_037298034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIA4"/>
    <dbReference type="GeneID" id="59335541"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF31">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="8">
        <location>
            <begin position="94"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="369"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6229027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="401" mass="45240" checksum="513D7643D866DD47" modified="2022-01-19" version="1">MAPFLEEPSTRPTYSGKPKIDGGILDLPNKKKHNIQYEPGRTIVEGHGEYEYDDLRPAFPDVHWEPLTEVPYHDRGTKGDPQFGNLLSDATNIFDYNPKIGTEISGINLAKLTDAQKDDLARLISTRGVVFFRNQTDLDIDAQRELGQYFGQLHKHATTSVPRRGDLDDVHVVYTDEKSKEQRAQFAPTFLWHSDVTYEVQPPSYTLLKMLTGPPRGGGGDTLWASQYAVYDLLSSGMQKYLEGLEALHSAQIQADGSIALGRTVRREPVVTKHPLVRTHPVTGWKSVFFNPGFVTDIVGIPKAEADAIIHYLNELITTTQEIHVRFQWQKNDVAFWDNRICNHSASYGFAPHRRHAVRVASQAECPYFDPNSKSQEEEMNARYGLPKVNKDGSGRSNYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIB5</accession>
    <name>A0A8H6FIB5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007152</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156680.1">
        <property type="nucleotide sequence ID" value="XM_037298045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIB5"/>
    <dbReference type="GeneID" id="59335552"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="TRNA(ILE)-LYSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033:SF1">
        <property type="entry name" value="TRNA(ILE)-LYSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="6">
        <location>
            <begin position="39"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="500" mass="56068" checksum="B2479C192B43E6DB" modified="2022-01-19" version="1">MGAVRVPKTPRIGIAVSGGVDSMALTALYLIEWELDVAQAETVLMRLASGHKGLGLHGMRSITDIPECWGVHGVHRSGTRDLAASGLRREEDKDPASPHAKGLQRILARDDIFEKGGVQILRPLLGFSKERLIQTCRAQDLAWEEDKTNKDAWRTPRNNIRELLHSAKLPQALQKTSMLHLAKQTSDNMHKVNTTVQRILSCCEILLLDVRSGRLIVRLPINQFLPRDDFERGKGWAVYEGQHRFTATLLLKRLVEIVTPQEEVSLQSLKQAAVSIFPELVGADRRLQPTSFTGGGAQFQRLHSPLPAPRSDLGPAISGISEDLDSVFVWKLTRQPFSEAPLSLTIQPSANTQSKIADNPPSWSSWQLWDGRYWIRLLNRSCWPLVVRSFQLADLKYLRSTFTPQRYKEFHQFLLLAAPDKVRWTLLAVAELADDTLPLGRVLALPTLGQAGIFDISDGNGTEKVDWQVRYKYVGLGYQLSDDSPGNISRNRSLITSWKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIZ7</accession>
    <name>A0A8H6FIZ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7708 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157011.1">
        <property type="nucleotide sequence ID" value="XM_037298376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIZ7"/>
    <dbReference type="GeneID" id="59335884"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056125">
        <property type="entry name" value="DUF7708"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24809">
        <property type="entry name" value="DUF7708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7708" evidence="1">
        <location>
            <begin position="82"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="199" mass="22211" checksum="F29E3AD0C0311E7A" modified="2022-01-19" version="1">MASTTPTPMRTKEAVKVDLWKQALATLNAEDQKQYEDCSSSMLDVLKQVQETTESKKQDCVSKGWEVYRNKQGEEVKLRHVLEKVSVWVKGIINIVDVGVFFDQSGHAALPWGIVKYLTTIGISDIDVFSSLVEGIESSASLVARYVIVEKLYLHYECEAAPGLQEAIGNLYANILTYLARVKAYFTGSTWSKCSTAFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJS6</accession>
    <name>A0A8H6FJS6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    <dbReference type="GeneID" id="59332584"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="129" mass="14223" checksum="9D2875FBE8C29881" modified="2022-01-19" version="1">MSTFTNDAFESHLPFEEISVCDAKDHGYNAWDHLFTREFRFDKGVTPRHSIIAGRIVKTFVNGGTHHSEPLFATLVDPLGANNGGEGTGKGYNTAKDTRAMMFIDPDKEDSGLMTLLLLVMQEASTKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJZ4</accession>
    <name>A0A8H6FJZ4_9LECA</name>
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        <recommendedName>
            <fullName evidence="7">OPA3-like protein</fullName>
        </recommendedName>
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        <name evidence="5" type="ORF">HO133_004217</name>
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    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the OPA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0005739">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="OPA3-like"/>
    </dbReference>
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        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07047">
        <property type="entry name" value="OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="73"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="245"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="251"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
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            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6229880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="35628" checksum="2B76EB9A82202673" modified="2022-01-19" version="1">MSSIVIKLGSLVVKTLSKPIANRIKAQAREHETFRRVCVSFAQGIHRWDMRFRLGLLQDSAALEKQAAREAAEAQAKKHKFEAPTVKTEAQTKADEASENRSKDGLDKAKEQRKKPKIRPLSEAKAIDLGSNFVSESFLFIVGVSLVFFETYRRSKQETSKREDVADQIEKLENQARLFRKAFVDLERETLKTTGTGLTGTRRILPREVYELEEEDEKEGKPKGWLSWFQGLYRRDVQADETKPEPLVRAPQSNPQSTSSVQNATTLNDSKSTSILSKILPTAHKDDTANNTQQLGVSPRNKTTESNSPPREGGSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK27</accession>
    <name>A0A8H6FK27_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004328</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MEF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157247.1">
        <property type="nucleotide sequence ID" value="XM_037295246.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59332736"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
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        <property type="entry name" value="Transcription factor, MADS-box"/>
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        <property type="entry name" value="TF_MADSbox_sf"/>
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        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
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        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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            <end position="225"/>
        </location>
    </feature>
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        <location>
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            <end position="301"/>
        </location>
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        <location>
            <begin position="314"/>
            <end position="473"/>
        </location>
    </feature>
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        <location>
            <begin position="493"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
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        <location>
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            <end position="204"/>
        </location>
    </feature>
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        <location>
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            <end position="225"/>
        </location>
    </feature>
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        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="319"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="336"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="392"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="505"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="526"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6229990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="551" mass="60125" checksum="CE557D8E249C5757" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK42</accession>
    <name>A0A8H6FK42_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004306</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157225.1">
        <property type="nucleotide sequence ID" value="XM_037295224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK42"/>
    <dbReference type="GeneID" id="59332714"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16180">
        <property type="entry name" value="EFh_PEF_Group_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="205"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="271"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="103"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6229968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="306" mass="34202" checksum="9472F5C1186552D6" modified="2022-01-19" version="1">MAYNSGYNPDALPAHAEPEQAAQILSSQQQRKQSNPLHPPPPRPYSNSNPQPYNLPQSQPQYQSKPPPPLPKPAQQLQTQPPPRLSPNNSYNNTQFNQNAAASPPPQNYGFGPPPQSHHGRPPPPSRPPGTPAPGGSSADASLFPLFKAVDTAGVGQLTERELGKALVNGDFTSFDPHTVKMMIRMFDADKDGSISFEEFCGLWGFLAAWRALFDRFDEDDSGYISFDEYSKALVAFGYRLSSAFVNLLYTTYARSGEDRMSFDLFVQSCIILKRMTDVFKRYDDDRDGYITLSFEEFLTEILRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYG5</accession>
    <name>A0A8H6KYG5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_007286</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156812.1">
        <property type="nucleotide sequence ID" value="XM_037298177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYG5"/>
    <dbReference type="GeneID" id="59335685"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15859">
        <property type="entry name" value="SNARE_SYN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000060">
        <property type="entry name" value="SNARE complex subunit (Syn8)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="176"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="31"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="37"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6229170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="29934" checksum="2F225BC5B95F0C1E" modified="2022-01-19" version="1">MANPSQLYLLADHIKLSLLERQRAISLNLEPNSQDGHISRSLDQMREGVEALEKSEGNDDSAADLRKQFDDLTSQFRGFPTTSKPSVSQPNDPALSPDFAAAKKIRPRNPSSSSFLKRSAADSPRPDKSVRFTDAPSDPQEEANRAALFPYRDEPEPGGPPDQSQLDNQQIHQYHSQVLRRQDEDLDRLGDSIGRQRDLSIQIGNELEDQIGMLDDSEQIMDRHQSRLDGARRQLGNVARRAKDNKQLTVIIILIVVLVLLIVILKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYS0</accession>
    <name>A0A8H6KYS0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Uridine kinase</fullName>
            <ecNumber evidence="8">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_007434</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the uridine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156960.1">
        <property type="nucleotide sequence ID" value="XM_037298325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYS0"/>
    <dbReference type="GeneID" id="59335833"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000339">
        <property type="entry name" value="Uridine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000010">
        <property type="entry name" value="Uridine-cytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="12"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6229318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="442" mass="50298" checksum="B53ED6A98754F7C0" modified="2022-01-19" version="1">MERAHYSPPWADTSIIGIAGSSGSGKSTLAHKIVSSLNLPWVAILSMDSFYRVLTPEQHVVAQRNEYDFDSPSSIDFDTLVERLQDLKAGRRAEIPIYSFEHHRRQDSTESLYSPHVLILEGIFALYDQRILDLLDLRIFAEADADVCLGRRITRDVRERGRDIEGCIKQWMSFVKPNFEKFVEPQRKVADIIIPARLDNRAAIDIVVGHVKRTLKEKSRKHQEELQRLGKQVEDEPLSENVLLLEQTREIVGMNTVIQNPMTAEVDFIFYFDRLSSLLVERAVENLHFNPKDVLTPQGYHYKGLEMAGEVSAVVISRGGSCFETGLRRVIPDCKCGRLLIQTSYRTGEPELHYWKLPSDIASHDSVLLLDPQMSSGGAALMAVKVLVDHGVSEEKVVFVTYFAGRMGVNRLLSVFPNMKLVVCKIVEDFQERWLEEKYFGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYV3</accession>
    <name>A0A8H6KYV3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156978.1">
        <property type="nucleotide sequence ID" value="XM_037298343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYV3"/>
    <dbReference type="GeneID" id="59335851"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="463"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="637"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="686"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="699"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="710"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="720" mass="81084" checksum="6DA868E238249A74" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZI4</accession>
    <name>A0A8H6KZI4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004229</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157149.1">
        <property type="nucleotide sequence ID" value="XM_037295148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZI4"/>
    <dbReference type="GeneID" id="59332638"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF108">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="4">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6229892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22151" checksum="C93B71EE4760811F" modified="2022-01-19" version="1">MPIGSSTAPCVISSAIYYLLKNPGCVTKAREEIDSVIGDGEFTHDHLESLPYCARIVRESLRLSAAAPGFNIEPLPDAQGPILLGGGKYHVPAKQTLIIVLHGVNRDPAVFERPETFNPETMVGEAFEKLPEGSKKWFGNGRRVCIGKDYAWMWNMTTLVTILKDVGIEMADPSYQLKQDGWFNLRPVDFYIKVKPRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZK5</accession>
    <name>A0A8H6KZK5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Mitochondrial phosphate carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004346</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transport of phosphate groups from the cytosol to the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157265.1">
        <property type="nucleotide sequence ID" value="XM_037295264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZK5"/>
    <dbReference type="GeneID" id="59332754"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035434">
        <property type="term" value="P:copper ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000076">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000131">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="85"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="182"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="283"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="47"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6230008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="41908" checksum="F118923914424449" modified="2022-01-19" version="1">MLFPTSSDINEVFSCRHPLTTTRSQESTPKSSQIKTARPQFSIWSVTEDAKSKANALSEEAQREIKKASAAAQAKTGQIELYSAKYYAACIFGGLVACGLTHTAVTPLDLVKCRRQVDSKMYKGNFEAWGKIGRAEGLRGIFTGWGPTFFGYSAQGAFKYGGYEFFKKFYSDLAGEQNAYKYKTSLYLTASASAEFIADIALCPFEAVKVRMQTTIPPFASGTFSGISTITAKEGTAGLYKGLYPLWGRQIPYTMMKFASFETIVEMIYGYMPGKKSDYGRGTQTAVSFAGGYAAGILCAIVSHPADVMVSKLNANRQPGEAFGAATGRIYKDIGFGGLWNGLVLRIFMIGTLTSLQWGIYDSFKIFMGLPTTGGAAPPPKQTANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZN8</accession>
    <name>A0A8H6KZN8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COX assembly mitochondrial protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004396</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for mitochondrial cytochrome c oxidase (COX) assembly and respiration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the CMC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157314.1">
        <property type="nucleotide sequence ID" value="XM_037295313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZN8"/>
    <dbReference type="GeneID" id="59332804"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013892">
        <property type="entry name" value="Cyt_c_biogenesis_Cmc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22977">
        <property type="entry name" value="COX ASSEMBLY MITOCHONDRIAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22977:SF1">
        <property type="entry name" value="COX ASSEMBLY MITOCHONDRIAL PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08583">
        <property type="entry name" value="Cmc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="49"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="80" mass="9379" checksum="5EA76EFDBE67E801" modified="2022-01-19" version="1">MHPHLHTKDNRGCEEVMAALDECHAQGFLHKVLGNCNGAKREVNKCLRAERLERTAKNREKAKEKKERIQRVWKEIDEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZQ1</accession>
    <name>A0A8H6KZQ1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004482</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157400.1">
        <property type="nucleotide sequence ID" value="XM_037295399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZQ1"/>
    <dbReference type="GeneID" id="59332890"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="310" mass="35857" checksum="5FF2C72EBE7389C2" modified="2022-01-19" version="1">MAGPKEEWLIWDRRFQFGYSCLNQRLKKFEAVTQQIAQLDQQTKHLTASSRHLQEDNDGLTDKIQQLQQASYQIAQLDEQIRDLAASSGIRKEENNELNNRILRLEQEGIRRDQENRLVQEQLIEKLSVQEGDFKSVAVAMKGMHEKARAERGQRGEEIQQLRSQVGDLIATRRTPGWDARDASSESSQATAEDGWMPSNQRAEQQRLNAAHQDIEVQGVLIRAQASFTLFENHLRYAEKAFERYETEAVHAFVNSAREEYRIALSNKLEEHGGEWTWQAAREEGHRIVEAEKKTRRRSTRFRAGSSRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZZ1</accession>
    <name>A0A8H6KZZ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004803</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157717.1">
        <property type="nucleotide sequence ID" value="XM_037295716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZZ1"/>
    <dbReference type="GeneID" id="59333209"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF22">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="679"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1114" mass="121702" checksum="F5C06628B8AAC8E8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6C8</accession>
    <name>A0A8H6C6C8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006904</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000028">
        <property type="protein sequence ID" value="KAF6217434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146869.1">
        <property type="nucleotide sequence ID" value="XM_037297800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6C8"/>
    <dbReference type="GeneID" id="59335304"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07730">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978:SF5">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="6"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6217434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="341" mass="38148" checksum="BE325DBBE9BB2CBE" modified="2022-01-19" version="1">MDIAAFAFLIEHNGQKLLFDLGVRKDWEVSSPPTLADPMKLGGWKVEVEKNVSEILQDNGTSLSSIDAIIWSHHHIAHTGDPSTFPPKTSLVVGPGFKRLFTPSYPDDETSPVLESAYAGREVREISFRGGFKLGRFPALDYFGDGSFYLLASPGHESGHMCGLARTTSSFAMQPQHMAGRDTFIFMGGNIAHHGGEFRPSVYRPLPSLLPDFPLFAPSFSSAVMPPCPGELLATIHCAQSATEPFYTSNCAEDQDKAIESIRRMMEFDARDTVFVVLAHDATLLSVIDCFPATANDWYAKEWAKKSRWLFLNDFEDDVKEYARNKKAREYEFDEKSLWMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C783</accession>
    <name>A0A8H6C783_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006198</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147672.1">
        <property type="nucleotide sequence ID" value="XM_037297098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C783"/>
    <dbReference type="GeneID" id="59334600"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="35991" checksum="24591A8EA5EEE948" modified="2022-01-19" version="1">MSFDYNLSKDEDLWDQAVKLDDRQKVDFSREAKREALLDIFVVAEKEKTSMKGRRWKLGKRSDGEPIVLRDVLGRIAGWIDSYKDVVINTVNYDPSMWLFHGLLYTIRSIENSLRGRQCLRKAIQWTPLRFAVENGHDEVVHLVWKSDPDVEAAAHDGKRALDPAVEMSQAKTLDIGLAADANIQAGVDDGRNPLMFALRNDRGLAIIKSLSNNRAELEATSGNARTALQWSVNRLGLMFANLSLSCSAKVNASERGGNTAEVSKQLDVDNGFFRGLQRLKMPSRGWKDADAGKLNESVETEEEWLERLGEHREAEYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7C2</accession>
    <name>A0A8H6C7C2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006386</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147409.1">
        <property type="nucleotide sequence ID" value="XM_037297284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7C2"/>
    <dbReference type="GeneID" id="59334787"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001317">
        <property type="entry name" value="Putative ADP-ribosylation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="147"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6217974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="216" mass="23649" checksum="E64566B891A71C1D" modified="2022-01-19" version="1">MYHLAKSVYFWATSKEEYSILLLGLDNAGKTTLLSTIKSLYNDTPAPAKPTVPTVGQNVATVSLDDMYLKIWDVGGQHALRSMWTSYYSVCHAIVFVVDGCDIGDDLGGGGGEGAGPVGRLEECRRTLESVLSHPSTASIPLLVLANKQDREDCVETVRIKEGLVRRVFEGERGGGVRDSRVLPVSALKGEGVREAVEWVRSRVRWNKEGRGPVMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7F6</accession>
    <name>A0A8H6C7F6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_006446</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147469.1">
        <property type="nucleotide sequence ID" value="XM_037297344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7F6"/>
    <dbReference type="GeneID" id="59334847"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF12">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14340)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="11">
        <location>
            <begin position="306"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6218034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="613" mass="68622" checksum="67F14ECAC5D75D66" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7X8</accession>
    <name>A0A8H6C7X8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005688</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147776.1">
        <property type="nucleotide sequence ID" value="XM_037296595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7X8"/>
    <dbReference type="GeneID" id="59334093"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF5">
        <property type="entry name" value="BTB_POZ DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="87"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="371" mass="41548" checksum="A1A8C679D7A15347" modified="2022-01-19" version="1">MVETAVRFYTPNGVMHFSPRATSPPHYPSPPPPPPSGHPTPSISPPPPLLSTISSPSKRQKTTAKMSEHRAGLVTGLANLLRTGEFSDLEILCGDRHLSVHKNVICCQSMPLKAMCTNGMKESQESVIDLTIDTEPCVSRLVDFFYRCDYDELGDDCEDVSTDDEDTAANNDNVAQWNLGSGKDDAGTYSDDASSDDFLPVPLIVTPRQLSLHIDMYVLADKYDVSSLAELAKKKFETMAEQSRPLQRKECFFPALETVPRIYAVTSEHNRGLRDVVVEYTRLQRTKSNNRDSFMFTDKVEELFESVPEFAADVLQSWLKMPYLGYCEEQDCGRILREQNIVCGSCGECGRNKNRIRTRDPSSQSLWTALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8T9</accession>
    <name>A0A8H6C8T9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ecp2 effector protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148540.1">
        <property type="nucleotide sequence ID" value="XM_037295842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8T9"/>
    <dbReference type="GeneID" id="59333336"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034906497" description="Ecp2 effector protein domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="28961" checksum="5B17082C5CE849DD" modified="2022-01-19" version="1">MISFTAFVLALSNIILVSALTLNSSSLILPGSAVANVTNSNYIHECNGIRFGYSLDSTSCTEALNQIDASSTTEQTYGARYKGSFDVKLPKRYISSDGRCIIEPNIILGEPSARASLQEVASAASYIIDQCVTARPSQGGKVRDIGGDNDLSVAVLKYEPKQVQCSGTVSPSLLSSCQNIVNTMPASMDFTTWGPENDPLAVMKLPLTYHSLDQRCKLSIRTAGPTDTFSRYQFWTVAVALTGVCVRNEQVGMRDGLGLGDHLSMEIGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8U4</accession>
    <name>A0A8H6C8U4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005606</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148497.1">
        <property type="nucleotide sequence ID" value="XM_037296515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8U4"/>
    <dbReference type="GeneID" id="59334012"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06179">
        <property type="entry name" value="MFS_TRI12_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010573">
        <property type="entry name" value="MFS_Str1/Tri12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053791">
        <property type="entry name" value="MFS_Tri12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF109">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06609">
        <property type="entry name" value="TRI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="438"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="539"/>
            <end position="559"/>
        </location>
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        <location>
            <begin position="55"/>
            <end position="563"/>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="587" mass="63091" checksum="917C6EEB87068F82" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC05</accession>
    <name>A0A8H6CC05_9LECA</name>
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        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002478</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037293404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC05"/>
    <dbReference type="GeneID" id="59330891"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054471">
        <property type="entry name" value="GPIID_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22939">
        <property type="entry name" value="WHD_GPIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="4">
        <location>
            <begin position="72"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase winged helix" evidence="3">
        <location>
            <begin position="354"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="570"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="625"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="682" mass="76984" checksum="AE4ADCDF972F73E0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC84</accession>
    <name>A0A8H6CC84_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="7 11">3.13.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_002570</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="10 11">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="7 11"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150325.1">
        <property type="nucleotide sequence ID" value="XM_037293496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC84"/>
    <dbReference type="GeneID" id="59330983"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
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        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10 11" id="KW-0520">NAD</keyword>
    <keyword evidence="4 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="13">
        <location>
            <begin position="195"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="161"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="226"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="303"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034527"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048858"/>
        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU004166"/>
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            <dbReference type="SMART" id="SM00997"/>
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            <dbReference type="EMBL" id="KAF6220890.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="450" mass="49097" checksum="299CC15FD684C120" modified="2022-01-19" version="1">MSGPPASKYKVADISLSAFGRREIELAENEMPGLMATRIKYAEDQPLKGARIAGCLHMTIQTAVLIETLTSLGAEVTWSSCNIFSTQDHAAAAIAAAGTPVFAWKGETEEEYNWCLEQQLVAFNDGKTLNLILDDGGDLTALVHNKHPEMLKDCYGVSEETTTGVHHLYRMLKGKGLLVPAVNVNDSVTKSKFDNLYGCRESLIDGIKRATDVMVAGKVAVVAGFGDVGKGCAQALHSMGARVIVTEIDPINALQAAVSGYQVTTMEDAAPEGQIFVTTTGCRDILLGKHFEVMKNDAIVCNIGHFDIEIDVAWLKKNAKSVQNIKPQVDRYLMENGHHIILLAEGRLVNLGCATGHSSFVMSCSFTNQVLAQIMLFKAEDEAFGKRYIEFGKTGKKEIGVYVLPKILDEQVALLHLDHVNVKLSKLSETQAEYLGLPAEGPFKNDIYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCG7</accession>
    <name>A0A8H6CCG7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chromodomain-helicase-DNA-binding protein 1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="ORF">HO133_003137</name>
    </gene>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
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            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037294061.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59331549"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025260">
        <property type="entry name" value="CHD1-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13907">
        <property type="entry name" value="CHD1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF4208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="827"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="851"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="Chromodomain-helicase-DNA-binding protein 1-like C-terminal" evidence="5">
        <location>
            <begin position="18"/>
            <end position="132"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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            <begin position="741"/>
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            <begin position="10"/>
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            <begin position="539"/>
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        <location>
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            <begin position="754"/>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="SMART" id="SM01176"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6220704.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="873" mass="97722" checksum="2A8DDDAF1F830B5A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCW1</accession>
    <name>A0A8H6CCW1_9LECA</name>
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        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="9" type="ORF">HO133_002011</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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        </lineage>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037292939.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59330425"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF37">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="18"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="208"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="473" mass="50174" checksum="D6756CB7994B2536" modified="2022-01-19" version="1">MAKSKPIGLKWRSSTLFIVSTVGIGLFTDLFLYGLVVPILPFILQDRVDLPPDQIQSHVSGLLAAYAGASVLFSPPAGILADRLPTRQLPFLVGLVALLLATLLLFLGQNVAVLTVARVLQGISAAVVWTIGLALVLDTVGPENLGKTIGSIFGFISIGELAAPVLGGIIYKSAGYGGVFGLGFAILALDFIMRILIIEKKTAAKYEDEEEEASNGHAAQPEQDAEDGDEETEEDPLIRKEQSQEYKIPLDQPKAIRSFPILYCLKDPRLLTALLLAFVQATLLATFDATIPTEAQELFNFDSLSAGLLFIALVLPYLVLGPVAGWAVDRYGPKPAAVIGFGYLVPVLILLRLVRSGGKSQIIVYCALLALCGLGMGVIGSPSIVEASYVVGMYDKSNPDLFGANGPYAQLYGINSMVFSAGLTVGPLISGSLKDAAGYGNMNLVVAALCLLTAILSFIYVGGKPAMLKKRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD63</accession>
    <name>A0A8H6CD63_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SGF29 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002022</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150603.1">
        <property type="nucleotide sequence ID" value="XM_037292950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD63"/>
    <dbReference type="GeneID" id="59330436"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20393">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20394">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037802">
        <property type="entry name" value="SGF29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010750">
        <property type="entry name" value="SGF29_tudor-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047288">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047287">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539:SF0">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07039">
        <property type="entry name" value="SGF29_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51518">
        <property type="entry name" value="SGF29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SGF29 C-terminal" evidence="6">
        <location>
            <begin position="192"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="107"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="324" mass="35789" checksum="43980455599D43C8" modified="2022-01-19" version="1">MAARNRPRGGLTKEDATDAHEERNMWSQIVTDIKRLRTTHAKAAEIAKAQVDMEAKIGKTPTVKEIDELLALYREGVRLAEEEEKILKEEPNDVIKNIEILAALRSASETEPVRPPQAVAKSRQVKRQKIETDGAADSPAPPIPSPSVVLPAARVKEIKNVRTGSVPREVKQEPIVKVEEGSEGSKGPAGERAGKFFVGAEVAYKQAKQKEDGSQWIQCNITSISEIANKKRYEVQDPEPDENGAPGQVYKTTAAALIAIPGPDAPLPDFPIGKQVLARYPETTTFYRAEVKGAKKDVYRLKFEDDQDQEMEVGRRFVLDVSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDP3</accession>
    <name>A0A8H6CDP3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chromatin associated protein KTI12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002131</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the KTI12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150711.1">
        <property type="nucleotide sequence ID" value="XM_037293058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDP3"/>
    <dbReference type="GeneID" id="59330545"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12435">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="32829" checksum="561632EBCE2B7DFC" modified="2022-01-19" version="1">MPLILISGYPASGKTYRSNQLVESFKSKIQASSDARISRLKVCHINHQSLGLDRHVYREARTEKDARATEYSAVKRALGKDTVVIADSLNYIKGFRYQLYCEAKAVHTPSCVVHVGTPISSCREINTRILESGSKDDGYPVDIFDNLVYRYEEPNGMTRWDSPLFTVPYDDEAPPCQDIWDAMVGSEGKAKTVKPNPATVMKPATESDYLYELDKISQGVLNVIMEWQKDHPGDGGGELAIAEGTIELPANPVSLPQLQRLRRQFISLNRLHNLPKARIRISFIEYLNDNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEC1</accession>
    <name>A0A8H6CEC1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001936</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151403.1">
        <property type="nucleotide sequence ID" value="XM_037292864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEC1"/>
    <dbReference type="GeneID" id="59330350"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="194" mass="22475" checksum="AEE34E9E3F3D87E6" modified="2022-01-19" version="1">MTIHRPSSSLPMEESEPTGPSWPPQSYKQISNEQRIGHQRSRVATVSKKPTSPEDQKPCAQREWRELPVWRLCLVIMLVSALVVYFCCSRDTYRRTLGKQKLPPEIHSANEYEPYAKELQSCKTTSYDLVFSCIEVVNSNLEYQKHAADKTDHIARTKHARDLHQCDMLLDQEQRKLEGLHGQLKQSSRLNGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEF4</accession>
    <name>A0A8H6CEF4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-D1-binding protein 1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001843</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151310.1">
        <property type="nucleotide sequence ID" value="XM_037292771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEF4"/>
    <dbReference type="GeneID" id="59330257"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026907">
        <property type="entry name" value="GCIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049317">
        <property type="entry name" value="GCIP-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492">
        <property type="entry name" value="CYCLIN D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492:SF1">
        <property type="entry name" value="CYCLIN-D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13324">
        <property type="entry name" value="GCIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like N-terminal" evidence="2">
        <location>
            <begin position="51"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="361" mass="39739" checksum="5443248A11A65770" modified="2022-01-19" version="1">MAPSKQSDALATLKTTILNLKTLLHRFHTALQSPTPVHPPIEDSPNPLALLSDASQILKAQTTKLSLLILNKPFTASAIVFILNSLSNSCLPAMMSALELCPAEKYTNLLHQYIQTSLSRMMMELQSLLALIPQDEHGIEKTGSRDILASTGVLWAECDKVVALGSGGLTRLAAERIEEFHGLLKDAIAELEEWDPDEESESDTDSLTSNKGTTSSAWPVDASLEKSLQGLTLSPVAALRKRTLVILRIIRLLYPALIKRRILTFSNINNSTTSESLPTSSHIQSLDALLDHTKQFTEETDEIAGALYAGDEEEVEDRLGNLAEISKICVEEVRIGWGGNEDEFTAWAEKWKARLEEVRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEF7</accession>
    <name>A0A8H6CEF7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001892</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Vacuolar effluxer which mediate the efflux of amino acids resulting from autophagic degradation. The release of autophagic amino acids allows the maintenance of protein synthesis and viability during nitrogen starvation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Vacuole membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ATG22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151359.1">
        <property type="nucleotide sequence ID" value="XM_037292820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEF7"/>
    <dbReference type="GeneID" id="59330306"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024671">
        <property type="entry name" value="Atg22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050495">
        <property type="entry name" value="ATG22/LtaA_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519:SF5">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11700">
        <property type="entry name" value="ATG22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="531"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="578"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="612" mass="67925" checksum="3CA779EAF0937ED5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEJ8</accession>
    <name>A0A8H6CEJ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001221</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151570.1">
        <property type="nucleotide sequence ID" value="XM_037292151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEJ8"/>
    <dbReference type="GeneID" id="59329637"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008610">
        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05890">
        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="310"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="386" mass="42849" checksum="A282FACA1DA8DEE5" modified="2022-01-19" version="1">MAKKGKLLTALDAHKGRDYNLQKQKKQQKQAAKKKRTKDPGSNSEEKEKEKENAEAQANGIPYTPGAESDGWESDESEAAEAIAIDTSRVIEDESDSDSSLGGEEAEEPDLEEAEDEDDIPLSDIESLAPEEKADILPHQRLTINNTTALTKSLKDIALPSSLHFSAHQSITTSEPVAILDVNDDLTRELAFYKQCLDAANEARTMLKNEGVPFSRPTDYFAEMVKSDEHMGKIKYKMVDEAANKKAAADARKQRDLKKFGKQVQVAKLQERDKAKRETLDRINLLKRKRKNTDTGAANEEDLFDVALEDAAKEERSSRSAKGGDGRGRTQNKRQKKDDKHGFGGKKRFSKSTDAASTADLRGFSAKKMKGKKGPQRLGKSRRAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF44</accession>
    <name>A0A8H6CF44_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001506</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151855.1">
        <property type="nucleotide sequence ID" value="XM_037292436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF44"/>
    <dbReference type="GeneID" id="59329922"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="12"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="303" mass="34347" checksum="F5D18C1EE55EC7BC" modified="2022-01-19" version="1">MANLDLRVADKYRLREKIGGGSFGAVYIGTHVDSGDEVAIKLEHVSVDPSFLECEADIYRSLSGGAGIPRVHAYVTECEYNAMVFDLLGPSLEDLFNFCGRKFSLKTVLMLVDQLLCRLEYIHAHDVIHRDIKPENCLMGVGKQGNQVYVTDLGLATERRPAQADANTGRSLRPSLVGTARFASVNGHLGVVQHRCDDLESLGYMLLYFIRPSLPWQGLKAANHEQREELILEKKQTISVEELCEGLPRAFAAYFDYIRSLGFDDKPKYSYLRKIFRDLFVREGFNHDNVFDWTILKYLQTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFI3</accession>
    <name>A0A8H6CFI3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000675</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151974.1">
        <property type="nucleotide sequence ID" value="XM_037291612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFI3"/>
    <dbReference type="GeneID" id="59329093"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF9">
        <property type="entry name" value="MEAB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="5">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="164"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="223"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="307"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="247"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="335"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="418" mass="46503" checksum="61B6B67BDBF6A85F" modified="2022-01-19" version="1">MSTIEDVPAAPKIEHFEEESFSESSPDPEEQSLTQEPAQVQKRKGGRKPIYATSEERKQRNRQAQAAFRERRTEYIKQLETTIKQHEEALQSLQQNHRAAADECLMLRYKNSLLERILLEKKIDVQAELKSKAEGATVVQLPTQPLGGPQPTPVQRALLNRLGQVRRSTSGSSSRALRPHSSKSSSVLSPQLQPSQHSQIVSAVTTKSPIGIAKGGMISPRVDMKAQQPQQPPPPPESQAYPQQQPSRLSIPTSRSSMQGTTPSTADTSRSSEMGSTAGTQTSFYPSPFQAHIEQLEQEYDAHVDMLDDENPSDRDVGPGPYPQNFHHQPLSPHQQIPMGMQGQPPMQQMQPMQQMQPMQPMQPPPASQAMDPNNPAYPGMQPGFDPYDPMLDADPFGLSASMHFPTQFTFQESSMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFI8</accession>
    <name>A0A8H6CFI8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit delta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001382</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151731.1">
        <property type="nucleotide sequence ID" value="XM_037292312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFI8"/>
    <dbReference type="GeneID" id="59329798"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233:SF14">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="502" mass="54154" checksum="E88750AA2F8975DE" modified="2022-01-19" version="1">MAPSESAQDDTMPSEKDRLDKKVTNGEQKVASMAEHIPGTNGTGEAAKGSEQGQKLADVPKEKELSGKEKKELAKAQKIAKRAAEKQKQQGQPAVDLVAGNKGELPKDSGKKGPLGPATPAKTQHKKTGSTSAGAQSSLPHRQALSHAAPAAAEPGKENKNVALFDHLYGHPRRTTVAGAGKDVHPAVLALGIQMRNYIVCGSSARCVAMLLVFKRVIESYTTPPRNSLPRHLTILLSSQIDYLVSCRPISISMGNAIRWLKVEISNVDVSTPESQAKSDLCDLIDTYVREKVTVADQVIANKANDKIEDGDVIMTFAKSSIVQQTLVEAFRRGKRFRVIVVDSRPLLEGKNLARALADLGLEVQYSLTHGISHVIRDTTKVFLGAHAMMSTGLYSRVGTALVAMSAKEANVPVIVCCESVKLTERVALDSFVHNEVAPAEELIIQGKPSSPLSKWVDVPNLQLLNLMYDLTPAEYINMVVTEYGIIPPSSVPVIIRLSTNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG32</accession>
    <name>A0A8H6CG32_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152274.1">
        <property type="nucleotide sequence ID" value="XM_037291912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG32"/>
    <dbReference type="GeneID" id="59329397"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032704">
        <property type="entry name" value="Cms1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030:SF0">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14617">
        <property type="entry name" value="CMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="177" mass="19772" checksum="A6126C64FAB90E3B" modified="2022-01-19" version="1">MSEKSKTSLSALPKSKVPATTIRLKSPFRTAKRKRNDGEDHDLEAVVDSSAKQKRSKKSKKTKSDEDHNLDLDQGLNLAIAKLDNRLLADYVAKRTKRFFPDLSLVELEDRYISETAFHDTTSWEKPRTLQDMASFLEHYNPKAAGNMALSSASKKLGSPHTLVITSSGLRAADITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG82</accession>
    <name>A0A8H6CG82_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152308.1">
        <property type="nucleotide sequence ID" value="XM_037291946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG82"/>
    <dbReference type="GeneID" id="59329431"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="13338" checksum="E286C976CFFE139F" modified="2022-01-19" version="1">MMDARWTDTNDIRAALPAIADSSSRPVSRTALTAFVRGTTELEPAVDAFTLMPHIESSEEKQIDEVTGGVEVAAVTSRLEGRDIQGEEYPTTQARPGEGPESHYMKPQAGDRSRFRPWGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGJ7</accession>
    <name>A0A8H6CGJ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-rRNA-processing protein IPI3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001021</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the WD repeat IPI3/WDR18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152316.1">
        <property type="nucleotide sequence ID" value="XM_037291954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGJ7"/>
    <dbReference type="GeneID" id="59329439"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000929">
        <property type="entry name" value="Ribosomal assembly complex component Ipi3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045227">
        <property type="entry name" value="WDR18/Ipi3/RID3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763">
        <property type="entry name" value="WD-REPEAT PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 5 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="124"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="183"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="494"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="502"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="516"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="526"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="547" mass="59849" checksum="D07DE1C001ED730E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHL7</accession>
    <name>A0A8H6CHL7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000536</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152910.1">
        <property type="nucleotide sequence ID" value="XM_037291475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHL7"/>
    <dbReference type="GeneID" id="59328955"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08243">
        <property type="entry name" value="quinone_oxidoreductase_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051603">
        <property type="entry name" value="Zinc-ADH_QOR/CCCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154:SF1">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="14"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="326" mass="34414" checksum="E695C7EBCBAFAD49" modified="2022-01-19" version="1">MAKTMRAAVMHAPGGPEVLQLENIPVPTPTAGRVLIRVKACGMNRSELFTRQGHSPGVRFPLVLGIEACGLVEECPGGEFKKGQIVATAMGGMGRVFDGGYAEYTCPPVGNVVAIETGLDWKVLGAVPEMLQTAYGSLFTALKLRKGERLLVRGGTSSVGLAAAAIAKNHGATVAGTTRKAGRDGLLKGSGCDHVVIDNGKVREEVRKIWPDGADKVLELVGTSSLVDSLSCVTRGGICCMTGMVSGQWSIPDFEVMAAVPTAVCLTTYQGDEKDFVSTPLEEMAQQIKEGSLKVSVGKTFPLEEIVEAHRTMEENRAGGKIVLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHP5</accession>
    <name>A0A8H6CHP5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF803 domain membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000581</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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            <begin position="21"/>
            <end position="43"/>
        </location>
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            <begin position="150"/>
            <end position="170"/>
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            <end position="196"/>
        </location>
    </feature>
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            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
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        <location>
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            <end position="262"/>
        </location>
    </feature>
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            <end position="325"/>
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            <end position="356"/>
        </location>
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        <location>
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            <end position="386"/>
        </location>
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            <begin position="56"/>
            <end position="78"/>
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        <location>
            <begin position="92"/>
            <end position="141"/>
        </location>
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        <location>
            <begin position="391"/>
            <end position="537"/>
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        <location>
            <begin position="565"/>
            <end position="662"/>
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            <begin position="64"/>
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        <location>
            <begin position="98"/>
            <end position="109"/>
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        </source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6223738.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="72147" checksum="0FE52ACFEA1904D7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI71</accession>
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        <recommendedName>
            <fullName evidence="16">Nuclear envelope protein</fullName>
        </recommendedName>
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        <name type="scientific">Letharia lupina</name>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDC1 family.</text>
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    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
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    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="21"/>
            <end position="40"/>
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        <location>
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            <end position="72"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6224023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="632" mass="70610" checksum="CB5650B20172AE71" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIA1</accession>
    <name>A0A8H6CIA1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">HO133_010536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153022.1">
        <property type="nucleotide sequence ID" value="XM_037301388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIA1"/>
    <dbReference type="GeneID" id="59338926"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046403">
        <property type="term" value="F:polynucleotide 3'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01625">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006551">
        <property type="entry name" value="Polynucleotide_phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01664">
        <property type="entry name" value="DNA-3'-Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083:SF9">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08645">
        <property type="entry name" value="PNK3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="477" mass="53070" checksum="FC979F7DA690E510" modified="2022-01-19" version="1">MTANHAAKREANAKEASLSPPPLKKRKVESTTTKRAVSSFFTPASKKEPERITWRIINESLLFASHQPDTADTRNRPRSKRPKIAAFDLDSTLIKTSSGNVFAKDATDWRWWHFSIPSVLKDLHADGYAIVILTNQGSISLRNDPKKIKSDQRSLANFKTKVASVLSHFDFPILLLAATARDCYRKPRIGAWKELLDNLDLDEGNGPVLQSSFFVGDAGGRAARTNAKADHACSDRDFASNVGITFHTPEEYFLHEEPVPFTRSFEPRTILNCSSSTSTHAMIIGRRNRLDIVLMCGSPGSGKSTFYWTKLKTLGYERINQDTLKTRDKCVKVASGMLAESKSVAIGTLPANLRSGNLEGLILEHLDNTNADEESRAVWVQLAHKFAVPIRCVHFPAPAKLCQHNDTVRALSTGTFNPEKRSILPHSAFTGFASRFTEPQLREGFQDVIKVEFQVAHRSGNLVLATLRWANVSFVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIW5</accession>
    <name>A0A8H6CIW5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_011007</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153490.1">
        <property type="nucleotide sequence ID" value="XM_037301856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIW5"/>
    <dbReference type="GeneID" id="59339397"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF7">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE (AFU_ORTHOLOGUE AFUA_2G00830)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="278" mass="30700" checksum="2D78A7E83AD09FF9" modified="2022-01-19" version="1">MPLHPVFKAGNSALITGAASGIGLAVAKFCNKHGMKVALVDRNAEALSEARLHFNNGIDTQIYAMDVSKIDEWRDLRVKVEMNFGQVSFVMLNAGIGLKSGWEDTEYFHKIMDTNLFGVINGISAFLPSLRTQSQPSSIVITGSKQGITNPPGNPAYNASKAAIKSLAEHLSYDLRATPTSVHLLVPGWTFTGLTGGGKTKEKPEGAWAPEQVADYLQQKMAEEVFYVICPDNDVTVEKDRKRMLWSVGDIINERPPLSRWREGYKDDAEEWMAKQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJZ2</accession>
    <name>A0A8H6CJZ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor Iwr1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010117</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IWR1/SLC7A6OS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153790.1">
        <property type="nucleotide sequence ID" value="XM_037300973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJZ2"/>
    <dbReference type="GeneID" id="59338509"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040150">
        <property type="entry name" value="Iwr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013883">
        <property type="entry name" value="TF_Iwr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28063">
        <property type="entry name" value="RNA POLYMERASE II NUCLEAR LOCALIZATION PROTEIN IWR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28063:SF1">
        <property type="entry name" value="RNA POLYMERASE II NUCLEAR LOCALIZATION PROTEIN IWR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08574">
        <property type="entry name" value="Iwr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor Iwr1" evidence="3">
        <location>
            <begin position="332"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="284"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="446" mass="50881" checksum="2909052FAFC3F2FF" modified="2022-01-19" version="1">MAFPPERITVKRRRDEDPVEALFIQQKKQRPSGFWKLVDDDNDTPQYAVPLSSSNAVKETRTTTTAFQSPRVPTVRTTLPSEDFDESNNAIPRFSKIKKPRAEASAIIDKRQLQSEITQHKQSRQKPQDILASALRARKFHLVINPSPISSPLSVPKTTAQKHRKNRRKDLAIFVERTEVIREAKKSGSVSRPSSGEPRHLDYIEKKDISQLEKPRKRPNATAAERKWRTETWANPPKPNEVTENLTRIAENINEPSSQWNYESTGLAEQLQEVVLEEIRASEERAKGLSGGGKLKVEPKPPKPRQPRTETSADERGGDDIMLDTMKLDDDGDYVLDIYVRSSAQPFRVMEPSELYHDSLNSIDPGNIGILVIEDEEEEALWEAFAEDQESGPEWNSEEEDENAEDYYGNDYPEDEVNSDDEFGRGAYNYRHAASDDEEFGWSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK96</accession>
    <name>A0A8H6CK96_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153860.1">
        <property type="nucleotide sequence ID" value="XM_037301043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK96"/>
    <dbReference type="GeneID" id="59338580"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990431">
        <property type="term" value="P:priRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990432">
        <property type="term" value="P:siRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051181">
        <property type="entry name" value="CAF1_poly(A)_ribonucleases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092">
        <property type="entry name" value="POLY A -SPECIFIC RIBONUCLEASE/TARGET OF EGR1, MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092:SF22">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE PNLDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="623" mass="70413" checksum="639B29EE2A6611EB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKG8</accession>
    <name>A0A8H6CKG8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ustYa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153628.1">
        <property type="nucleotide sequence ID" value="XM_037300811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKG8"/>
    <dbReference type="GeneID" id="59338347"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021765">
        <property type="entry name" value="UstYa-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33365:SF6">
        <property type="entry name" value="OXIDASE USTYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33365">
        <property type="entry name" value="YALI0B05434P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11807">
        <property type="entry name" value="UstYa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00035112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="202" mass="23046" checksum="065C19E9725D950F" modified="2022-01-19" version="1">MSAGLSRDVPTTFERGGVYFDENETISNAAWDAIEYYHGTVALDHDYAASKGLPRSQNFPWDDTKGIYFLNGFHDLHCVKRIRRTMLQLRDGVPLTSPVSHSLHCLNSLRQDILCAADDTPLYTSYDHPGAVGFDQPRQCRSWEALEDWANERTACWRDINSGQHIDTLFRYRYCPEGSPYNEHIQAIFGEFEKGENASAFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKK0</accession>
    <name>A0A8H6CKK0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010482</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154151.1">
        <property type="nucleotide sequence ID" value="XM_037301334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKK0"/>
    <dbReference type="GeneID" id="59338872"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="563" mass="64787" checksum="0305CC1F950C7493" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKK2</accession>
    <name>A0A8H6CKK2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mannitol-1-phosphate 5-dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010015</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-mannitol 1-phosphate + NAD(+) = beta-D-fructose 6-phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19661"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:61381"/>
            <dbReference type="EC" id="1.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the mannitol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153688.1">
        <property type="nucleotide sequence ID" value="XM_037300871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKK2"/>
    <dbReference type="GeneID" id="59338407"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008926">
        <property type="term" value="F:mannitol-1-phosphate 5-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019592">
        <property type="term" value="P:mannitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00196">
        <property type="entry name" value="Mannitol_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023028">
        <property type="entry name" value="Mannitol_1_phos_5_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000669">
        <property type="entry name" value="Mannitol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013118">
        <property type="entry name" value="Mannitol_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013131">
        <property type="entry name" value="Mannitol_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002647">
        <property type="entry name" value="PRK02318.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002652">
        <property type="entry name" value="PRK02318.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30524:SF0">
        <property type="entry name" value="ALTRONATE OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30524">
        <property type="entry name" value="MANNITOL-1-PHOSPHATE 5-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01232">
        <property type="entry name" value="Mannitol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08125">
        <property type="entry name" value="Mannitol_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00084">
        <property type="entry name" value="MTLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mannitol dehydrogenase N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Mannitol dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="207"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="388" mass="42620" checksum="34AE0A189C782D9F" modified="2022-01-19" version="1">MGLKAVHFGGGNIGRGFVAEKLCQSGFEVVFVDVMDKLIEQLQQTKSYTVTEIGGEGEKVNTISNYRAINSKTHEKDAVQEISSADLVTCAVGPNILKFIAPVIAKAIDSRTASTPIAVIACENMIGATDNLAGFIKDPKNTPEDRLSSISERARFANSAIDRIVPGQDPDAGLNVKIESFYEWVVDKTPFAPHGPPKVEAIKWVDDLVPYIERKLFTVNTGHAAAAYYGHARGKKTIADALEDKEIHEIVRECLAETSHLIVNKHAISVSEQKDYVEKIIKRISNPHLEDVVERVGRAPLRKLGRQERFVGPAHHLAEQGDKYTALLGAMEQAFNFVDVEGDDESKELSKILKSMDAPSVVSKVCGLEKSDQMFGQVQAIVERVQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKW2</accession>
    <name>A0A8H6CKW2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010266</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153938.1">
        <property type="nucleotide sequence ID" value="XM_037301121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKW2"/>
    <dbReference type="GeneID" id="59338658"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF2">
        <property type="entry name" value="ALPHA_BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="147"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="390" mass="44596" checksum="BB66731C11DDB59F" modified="2022-01-19" version="1">MILDQISYLDCLVFLFFLTPQLLLRVSVFELLVCVVQALPFFLIQLPYEFIEERYFTPKRSRSPFVKQASPFQDIVIRIVRYAFAHFSTKIGRVFFSKPVALPFLRFRMLRRGYLKSPITWLEIDLKDTHGVWIIVDPYDKPDIVIYHCHGGGFSMGSCYFYLEFLIAWVSLLKEAGFRNPAIFSLEYTLVPDAVYPAQLEQTITGYEYVRKIVRDPSRICVGGDSAGATLILSLLLHIARQPDYENRRPGYAALISPWTTLFSTQNRNTASDFLDANRLHMYGRQYAGSDENLDDPLVSPGVCNDCTWWAKASPSNGYCILYGSEEVFGPDLRDLIALWRKSGCAVSVREDGAVHAWPVASMFLCDTQAERQKGLRDLVKMIKQAIKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKY6</accession>
    <name>A0A8H6CKY6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154173.1">
        <property type="nucleotide sequence ID" value="XM_037300325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKY6"/>
    <dbReference type="GeneID" id="59337859"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="242" mass="26903" checksum="A4EA72F9073CEE91" modified="2022-01-19" version="1">MEHQYLLSPHVDASPSPVSDVPSPVSDISPLESKHKAIIIFFKYFVDVATTKASTSTAETERLLPESSAAASEFLKRLYNVEVRASSIDRYWRQAKENAVERNITWDENAVAQIISSQVKDDDTFDEVNSFLQHHAGVSSSEPIFFLSLASADSSHKDERLSVTTEWIISKAWQALSDPQGNVPLRTYWKEINRGRRGPPSIPDPPLKQVSDESPTPLVIPSSLLQRDADRALENGRSGDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLA3</accession>
    <name>A0A8H6CLA3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154186.1">
        <property type="nucleotide sequence ID" value="XM_037300338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLA3"/>
    <dbReference type="GeneID" id="59337872"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF600">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G02580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="103"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="373" mass="41490" checksum="5E67331E1E15A644" modified="2022-01-19" version="1">MSTQIRPVPSNEGPSKTIPIPANHQRKPHPRWWLSAQANVWRFLGQVGLYFHSIPTPSPKEPSFLRKFPTTVVSGQATMLHLAFYVPDGYQSQIQQGRKYPLVVNMHGGGFTLGTAKDDGRWATMVVKQVEAVFVSVEYRLAPEYPFPTAVEDGVEALLHLAANADELGLNPHKMALTGFSAGGNMAFTMPLRLRTHIQSTRNEYQSKPMEASQSNGTDRTLSGPPLPEIVSIIAWYPSLDNRLTRDERRASCVRPGKTLPPILTSLFDESYLPELENRMSPYASPAAATDADLTAAIPEGVAMYLCEWDMLLQEGEVFAERLKGLGKRVHLVIIKEKRHGFDKSPYPFSVDPEATLHYTEACTILKDALFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLG4</accession>
    <name>A0A8H6CLG4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009565</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154274.1">
        <property type="nucleotide sequence ID" value="XM_037300426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLG4"/>
    <dbReference type="GeneID" id="59337960"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF54">
        <property type="entry name" value="FAMILY OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="3">
        <location>
            <begin position="103"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="410" mass="46527" checksum="2C3B74AD1BAC68C0" modified="2022-01-19" version="1">MATVGYPTHANRVATEAPSVLSYYPTTGDALRFWSLREDKNVHSEVPQGFPAHIDARMAWQGSDMEMQSDAWVVLLSEADVFALEGAMRDFRNQALHLSQIDQISFRLPDQLAKRLDDISEECYNGRGFCVIRGLEPAKYTDEENVILYAGISAYIAPERGFLDRARQHVLCHVLPAAFSAEQDRKITPGFTNDAMSFHTDDGDILALYAMNTDQSGGRTQIASGWKVYNELASSRADVLHTLTQDWVLDTYQDYSQWPPIKTPLLFTDGKKVMFNFSRYPLCGFRGNKRNQSLPAPTESQLEALDAVHFFAEKNSMSLPTKTGDILYLNNMSMLHAREPFEDQGTQGMGSRRHMLKMQLRDPKRTWRLPNNLVESWKLLFAPNREDGGKDERFMITPEEGHERGWSSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLU1</accession>
    <name>A0A8H6CLU1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_009805</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154512.1">
        <property type="nucleotide sequence ID" value="XM_037300664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLU1"/>
    <dbReference type="GeneID" id="59338200"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120104">
        <property type="term" value="C:mitotic actomyosin contractile ring, proximal layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106006">
        <property type="term" value="F:cytoskeletal protein-membrane anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07651">
        <property type="entry name" value="F-BAR_PombeCdc15_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000045">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000164">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF7">
        <property type="entry name" value="NOSTRIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="10">
        <location>
            <begin position="13"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="825"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="378"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="588"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="408"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="431"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="614"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="669"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="725"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="761"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6225803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="885" mass="97252" checksum="5E928ADAC9128E7F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM02</accession>
    <name>A0A8H6CM02_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA helicase HEL117</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154531.1">
        <property type="nucleotide sequence ID" value="XM_037300683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM02"/>
    <dbReference type="GeneID" id="59338219"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044688">
        <property type="entry name" value="SCI-1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34117">
        <property type="entry name" value="STYLE CELL-CYCLE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34117:SF1">
        <property type="entry name" value="STYLE CELL-CYCLE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="38354" checksum="E1392500C8A69510" modified="2022-01-19" version="1">MSSREQQNIARRSRSPHNRRHHKRSRSPNSHLYHHVSHKHKQSKPSAPVHLPLQASKLHKHDFETVKPMFELYLDIQKQKVLEALPDDEVKGRWKSFVGKWNRAELAEGWYDPATLQKAQASAASNAVNTERQNQPRDSPSYGSPRRAEESSDEDVVGPTMPGGETQVYKSGRRPGPVIPNPQDLELRRELEQEDSLAHRQDLREHRHLDRRQQRERLDELVPRAEAGSKDRILEKKREKADSNSAFASAKMEAGGVEEVPESDLLGDEDGGPEGFKKQKKEMDRKKNEREVRREEILRARMVELEERRREYRAKEEKTMSGLVALAKARFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM72</accession>
    <name>A0A8H6CM72_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008947</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154635.1">
        <property type="nucleotide sequence ID" value="XM_037299809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM72"/>
    <dbReference type="GeneID" id="59337342"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="327" mass="37304" checksum="19CB1845458848F1" modified="2022-01-19" version="1">MRTELLSECGLLSDRRKPRFVITCDELPAFCRSLRFLDKGIPGSLASIQLRLIIGDEFGTDGAKVGEDEATESPGLTAAAIERSYASMNNTIATTSSRVRRLLEPFRRLHSMNNLQIIGSIPEKYKAEVIADISKPLPKNQEPCDHVLTCFKEAISKNDCGDFATGDVETEIMAGPYARCTIWNAYEHIEVTAWTKIAWAWLKTGDVHTADRWLSRIVQKFVYRQRDNGNMPAGEHDIAMVFHLAYQILEERDRMGSVGVTIFRTPLGRLKRWLRHEPENSLLTQGLEKRELDLAKEQDIHDSMNMADRLDPESDIEILKWVWNQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMK1</accession>
    <name>A0A8H6CMK1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154580.1">
        <property type="nucleotide sequence ID" value="XM_037300732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMK1"/>
    <dbReference type="GeneID" id="59338268"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13563">
        <property type="entry name" value="2_5_RNA_ligase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="199" mass="22651" checksum="29D61DFAEEAACEB9" modified="2022-01-19" version="1">MTTSNSHQSSSAPYTADKYARRADHTPNTAQEEEDSYILALHTDPEHHKRVTALRTQYFPPKLNKLSAHIALFRALPGSHLATIEADISAAAERQQSFPIATGKVYLLGHGVAIDVNSPPSRAIYNGLKEKWEDFLSKQDKSFKAHYTIQNKVEDGVPQRTLEELQKNFEGSQGQVDGLSLYRYDRGHWRHMRDFMFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMV6</accession>
    <name>A0A8H6CMV6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetylglucosamine-induced protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009209</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154896.1">
        <property type="nucleotide sequence ID" value="XM_037300070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMV6"/>
    <dbReference type="GeneID" id="59337604"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022036">
        <property type="entry name" value="DUF3605"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020:SF4">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12239">
        <property type="entry name" value="DUF3605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="28678" checksum="A8EDE440C69FD278" modified="2022-01-19" version="1">MAPSNPTDSAVATLSSYRAGKRPLPYWLLNVPEPQWPSQCPPFLTDISPRNLEIVNVPEAAFRRLTWPEVRRTIRANRIEEFRRAPLDHRNYLCYMARLKEEHGSVMEYVRDRRLRWTDLDAKGLAFEDPDDVKIIYNDWPYGIDKSIVHLCVWTKFPLDVDEKDPNGDLTPEMRGKIDGYVERTFGSRVPAENIIWFKNWAKLKSIHSMEHFHVMMKDPDMEFIKEITKGDSPASSQLSESTILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN15</accession>
    <name>A0A8H6CN15_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Urease</fullName>
            <ecNumber evidence="2 9">3.5.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">Urea amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_009334</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>urea + 2 H2O + H(+) = hydrogencarbonate + 2 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:20557"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Ni cation</name>
            <dbReference type="ChEBI" id="CHEBI:25516"/>
        </cofactor>
        <text evidence="11">Binds 2 nickel ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="10">Carbamylation allows a single lysine to coordinate two nickel ions.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.5" evidence="2 9"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155021.1">
        <property type="nucleotide sequence ID" value="XM_037300195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN15"/>
    <dbReference type="GeneID" id="59337729"/>
    <dbReference type="UniPathway" id="UPA00258">
        <property type="reaction ID" value="UER00370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035550">
        <property type="term" value="C:urease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009039">
        <property type="term" value="F:urease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00375">
        <property type="entry name" value="Urease_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00407">
        <property type="entry name" value="Urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00390">
        <property type="entry name" value="Urease_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.280.10:FF:000001">
        <property type="entry name" value="Urease subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.150.10">
        <property type="entry name" value="Urease, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.280.10">
        <property type="entry name" value="Urease, gamma-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01953">
        <property type="entry name" value="Urease_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008221">
        <property type="entry name" value="Urease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011612">
        <property type="entry name" value="Urease_alpha_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050112">
        <property type="entry name" value="Urease_alpha_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017950">
        <property type="entry name" value="Urease_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005848">
        <property type="entry name" value="Urease_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017951">
        <property type="entry name" value="Urease_asu_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002019">
        <property type="entry name" value="Urease_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036461">
        <property type="entry name" value="Urease_betasu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002026">
        <property type="entry name" value="Urease_gamma/gamma-beta_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036463">
        <property type="entry name" value="Urease_gamma_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029754">
        <property type="entry name" value="Urease_Ni-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009671">
        <property type="entry name" value="PRK13192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009686">
        <property type="entry name" value="PRK13207.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01792">
        <property type="entry name" value="urease_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00192">
        <property type="entry name" value="urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00193">
        <property type="entry name" value="urease_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43440">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43440:SF1">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00449">
        <property type="entry name" value="Urease_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00699">
        <property type="entry name" value="Urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00547">
        <property type="entry name" value="Urease_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001222">
        <property type="entry name" value="Urease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01752">
        <property type="entry name" value="UREASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51278">
        <property type="entry name" value="Urease, beta-subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54111">
        <property type="entry name" value="Urease, gamma-subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01120">
        <property type="entry name" value="UREASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00145">
        <property type="entry name" value="UREASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51368">
        <property type="entry name" value="UREASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0533">Nickel</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Urease" evidence="14">
        <location>
            <begin position="400"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12 13">
        <location>
            <position position="591"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="11">
        <location>
            <position position="488"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="11">
        <location>
            <position position="488"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="517"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="631"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="10">
        <location>
            <position position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001222-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001222-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR611612-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6226468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="836" mass="90252" checksum="1FC1D72C2174DDB4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNI2</accession>
    <name>A0A8H6CNI2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009217</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154904.1">
        <property type="nucleotide sequence ID" value="XM_037300078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNI2"/>
    <dbReference type="GeneID" id="59337612"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056030">
        <property type="entry name" value="DUF7611"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056031">
        <property type="entry name" value="DUF7612"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056032">
        <property type="entry name" value="DUF7613"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056033">
        <property type="entry name" value="DUF7614"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24586">
        <property type="entry name" value="DUF7611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24587">
        <property type="entry name" value="DUF7612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24588">
        <property type="entry name" value="DUF7613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24589">
        <property type="entry name" value="DUF7614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7611" evidence="2">
        <location>
            <begin position="517"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7612" evidence="3">
        <location>
            <begin position="672"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7613" evidence="4">
        <location>
            <begin position="799"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7614" evidence="5">
        <location>
            <begin position="962"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1123" mass="125775" checksum="50FFC83D70F1AFE5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNU0</accession>
    <name>A0A8H6CNU0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155039.1">
        <property type="nucleotide sequence ID" value="XM_037300213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNU0"/>
    <dbReference type="GeneID" id="59337747"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF67">
        <property type="entry name" value="AMIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="136"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="599" mass="63819" checksum="25397D516681A6F3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP89</accession>
    <name>A0A8H6CP89_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha N-terminal protein methyltransferase 1</fullName>
            <ecNumber evidence="5">2.1.1.244</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Translation associated element 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">X-Pro-Lys N-terminal protein methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_008489</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54712"/>
            <dbReference type="Rhea" id="RHEA-COMP:13785"/>
            <dbReference type="Rhea" id="RHEA-COMP:13971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138057"/>
            <dbReference type="ChEBI" id="CHEBI:138315"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54736"/>
            <dbReference type="Rhea" id="RHEA-COMP:13787"/>
            <dbReference type="Rhea" id="RHEA-COMP:13974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138059"/>
            <dbReference type="ChEBI" id="CHEBI:138318"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54724"/>
            <dbReference type="Rhea" id="RHEA-COMP:13789"/>
            <dbReference type="Rhea" id="RHEA-COMP:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138061"/>
            <dbReference type="ChEBI" id="CHEBI:138317"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. NTM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.244" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155356.1">
        <property type="nucleotide sequence ID" value="XM_037299355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP89"/>
    <dbReference type="GeneID" id="59336885"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000025">
        <property type="entry name" value="N-terminal Xaa-Pro-Lys N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008576">
        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753">
        <property type="entry name" value="AD-003 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753:SF0">
        <property type="entry name" value="ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05891">
        <property type="entry name" value="Methyltransf_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016958">
        <property type="entry name" value="DUF858_MeTrfase_lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6227048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="231" mass="25789" checksum="B47932F2BB79AA27" modified="2022-01-19" version="1">MTPSTNQAPDANISTLDSLKYWNSISTTVDGMLGGYPEISRTDLKGSANFLAKLRREQTSRSADGPLQRGVDCGAGIGRITAGFLSTVCEVVDVVEPVEKFAREVKGQKMIGSGSLGSVYVAGLEDWVPEAQYDLIWNQWCLGHLTDKQLVAYLERCKNAVTIDGWIIVKENMSTNVDGEDFFDQQDSSVTRTDQKFKQLFKEADMRLIRTEVQRGFPKGLYPVRFYALKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPD6</accession>
    <name>A0A8H6CPD6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008691</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155557.1">
        <property type="nucleotide sequence ID" value="XM_037299556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPD6"/>
    <dbReference type="GeneID" id="59337086"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677:SF1">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677">
        <property type="entry name" value="YALI0B19030P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="374" mass="42221" checksum="8E4920EB9D00F682" modified="2022-01-19" version="1">MATAEVESLMDDTRAHVGPVIKAMSMNTLSTDNFDPKKHIDFTPPAKTLTMSDLKLPEGTGVSPFAVSDPFQLFTQEAVQRMRAEIFSKEVLENCKYSSNLAQCQLRGFAAKYAPFVYDAWKNPETLAIMSNIAGVQLVTEMDFEIAHINISVKSEKQKAEELEAFLDRKLTEDDEGIAGCPWEDDKPIVDWHTDSYPFVCVTMLSDCTNMIGGETVLRTGNGDVIKVRGPQMGCAVVLQGRYIEHQALRALGSTERVTMVTSFRPKCHTFRDDTVLTTVRPVSNLSELYSQFCEYRLEILEERIRNQLKAMRDAERSGRKMSTKALKAFLHDQELFLAHMNKEMIDDEFVTKGSIDNSHLFSDFAESNRARVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPK4</accession>
    <name>A0A8H6CPK4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008432</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155299.1">
        <property type="nucleotide sequence ID" value="XM_037299298.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336828"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041068">
        <property type="entry name" value="HTH_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF21">
        <property type="entry name" value="NON-REDUCING POLYKETIDE SYNTHASE AUSA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18558">
        <property type="entry name" value="HTH_51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="10">
        <location>
            <begin position="382"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="11">
        <location>
            <begin position="1276"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="9">
        <location>
            <begin position="1627"/>
            <end position="1703"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="7">
        <location>
            <begin position="1276"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1376"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="7">
        <location>
            <begin position="1432"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1608"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1696"/>
            <end position="1754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2125"/>
            <end position="2158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1376"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1608"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1707"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1731"/>
            <end position="1744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2136"/>
            <end position="2152"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="7">
        <location>
            <position position="1311"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="7">
        <location>
            <position position="1495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6226991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2216" mass="240952" checksum="F290DFD290CD014D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ15</accession>
    <name>A0A8H6CQ15_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="6">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008882</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155746.1">
        <property type="nucleotide sequence ID" value="XM_037299745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ15"/>
    <dbReference type="GeneID" id="59337277"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000002">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF1">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ERV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="7">
        <location>
            <begin position="66"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="200" mass="22072" checksum="210BCE87953CEB36" modified="2022-01-19" version="1">MPTPGTVPNRRFLLLLVTAVGLVSLLTWTFSGRRNLDATFSSIPIHQVSVDDDVLKGGAIMGKIGNETIKAELGRAAWKLFHTTMAKFPDKPSQDEQTALFSYIHLFARLYPCGECAGHFRQILNKFPPQVSSRSSAAAWACHVHNEVNKSLGKDLFDCSKIGDFYDCGCAEEDEKKDKKVTNTDRIDLQVEKLGPTKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQC3</accession>
    <name>A0A8H6CQC3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome subunit beta type-6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008754</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155619.1">
        <property type="nucleotide sequence ID" value="XM_037299618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQC3"/>
    <dbReference type="GeneID" id="59337149"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03757">
        <property type="entry name" value="proteasome_beta_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000027">
        <property type="entry name" value="Proteasome subunit beta type-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6227311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="28947" checksum="E73961B1D6E5B8DC" modified="2022-01-19" version="1">MASILGANPTLTSIPSYSFSQASHNNGATGNKEHAFYPYTDNGGSTLGITGNDFAIIAGDTRLTSGYSINTRYSPKVFRIGGDDETGEGAHIILSVVGFAADGTALKERLNAIVKMYKYQHGKKMSVKACAQRLATILYGKRFFPYYVTAILGGLDEDGKGALYSYDPVGSYEREQCRAAGAAASLIMPFLDNQVNFKNQYLPSTGEGHDLLARQAEPLPRHEVEQLVRDAFTSATERHIEVGDGLQMLVITQEGIEEAFFPLKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQM1</accession>
    <name>A0A8H6CQM1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_008905</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155769.1">
        <property type="nucleotide sequence ID" value="XM_037299768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQM1"/>
    <dbReference type="GeneID" id="59337300"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09769">
        <property type="entry name" value="Luminal_IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10422">
        <property type="entry name" value="RNase_Ire1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000077">
        <property type="entry name" value="Putative Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000572">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.180">
        <property type="entry name" value="KEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010513">
        <property type="entry name" value="KEN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038357">
        <property type="entry name" value="KEN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954">
        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954:SF6">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06479">
        <property type="entry name" value="Ribonuc_2-5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51392">
        <property type="entry name" value="KEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034362159" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="29"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="739"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="domain" description="KEN" evidence="22">
        <location>
            <begin position="1048"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="566"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="609"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="566"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="626"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="663"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAF6227461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1187" mass="131935" checksum="1A4A4B5CBC244358" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR06</accession>
    <name>A0A8H6CR06_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WD-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007617</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155823.1">
        <property type="nucleotide sequence ID" value="XM_037298487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR06"/>
    <dbReference type="GeneID" id="59336014"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046925">
        <property type="entry name" value="WD-like_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20493">
        <property type="entry name" value="WD-like_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034473376" description="WD-like domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="WD-like" evidence="2">
        <location>
            <begin position="125"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="231" mass="24147" checksum="43EDC48238BABE3E" modified="2022-01-19" version="1">MFSSAIIPSLLLGLSQIHSSIAQPVNASSVTVTLAASDHFPHAKGDLSPFDVDVNYVGYNASTQSWLTSLNPASGASDEEKARIMTEAAYAKPFDANDPDMTADVNSLLATIAGNATALEKRDGSSFKVVAGHAVKWAACAKVFGCLSGSTCTFTLNVGKAPRSQCQSQGGSNCCISWSDYNVRFGFFTTTWTTCNDEVTAQQMSTASCEGYGSSSQGGDVCLSNRANGCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR24</accession>
    <name>A0A8H6CR24_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Catabolite repression protein creC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007813</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the regulatory network controlling carbon source utilization through ubiquitination and deubiquitination involving creA, creB, creC, creD and acrB. Required to prevent the proteolysis of the CreB deubiquitinating enzyme in the absence of carbon catabolite repression. CreB deubiquitinating enzyme stabilized in a complex with the CreC leads to the expression of genes such as those in the proline and quinate pathways.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with creB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat creC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156019.1">
        <property type="nucleotide sequence ID" value="XM_037298683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR24"/>
    <dbReference type="GeneID" id="59336210"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045013">
        <property type="term" value="P:carbon catabolite repression of transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051362">
        <property type="entry name" value="WD_repeat_creC_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107:SF16">
        <property type="entry name" value="AT02583P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="414"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="114"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="518"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="606" mass="67157" checksum="0E036D00FC8FBEB3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRQ4</accession>
    <name>A0A8H6CRQ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008194</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156398.1">
        <property type="nucleotide sequence ID" value="XM_037299062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRQ4"/>
    <dbReference type="GeneID" id="59336591"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="581"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="724"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="806" mass="87872" checksum="42151F37479F262E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRT7</accession>
    <name>A0A8H6CRT7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156424.1">
        <property type="nucleotide sequence ID" value="XM_037299088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRT7"/>
    <dbReference type="GeneID" id="59336617"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="386" mass="42499" checksum="54E43770FA34AFA0" modified="2022-01-19" version="1">MEMSLDILPNADDNRARDLEVIHLNRMSSNISSSADQNKARGVQQTPRDALNNKIDELEQRLAALTADISPSRSSNASFRTAQEQTSPTPLSQNALGKRKEQDSPKRPNPVSFPPRSSRRQSYTNTQASSRPTTSHRASTRPSTRRNSTAPTSQVPSASSSRREGRAPTTTRPSASSSRRSSFRVNQPASTQTSRPARLNPRNNTTWPPPTVLRDGKQALQSLRAFSDLRDDVYSAMQAEERLKSSSLPTLPRPTTADTTAASSVSAANDVGTGSSQDRHEYSNHVPAANIDWTSASTRRREYEKIDQARSGLRGFIRRVTPRILRRKTGRVGFHDDGKGSDAGTVRRFRMDVADGDSAVDESAKSHWWKFGKKKAKGKGTSSTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CU05</accession>
    <name>A0A8H6CU05_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006982</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156513.1">
        <property type="nucleotide sequence ID" value="XM_037297878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CU05"/>
    <dbReference type="GeneID" id="59335382"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="72" mass="8016" checksum="C213B337681190D4" modified="2022-01-19" version="1">MTKRTKKVGVTGKYGTRYGASLRKQVKKMEISQHARYTCTFCGKTTVKRHSVGIWSCRSCKKTVAGGAWTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6Y3</accession>
    <name>A0A8H6F6Y3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147182.1">
        <property type="nucleotide sequence ID" value="XM_037297472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6Y3"/>
    <dbReference type="GeneID" id="59334975"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1109"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1109"/>
            <end position="1133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1148" mass="127548" checksum="709A2BADFE7B9E86" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F792</accession>
    <name>A0A8H6F792_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006307</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147330.1">
        <property type="nucleotide sequence ID" value="XM_037297205.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59334708"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000023">
        <property type="entry name" value="Sensor histidine kinase/response regulator, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000083">
        <property type="entry name" value="Sensor histidine kinase/response regulator, putative (AFU_orthologue AFUA_4G00660)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF72">
        <property type="entry name" value="HISTIDINE KINASE_RESPONSE REGULATOR, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G06140)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="509"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="1169"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="246"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="395"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="797"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1018"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1135"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="407"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="803"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1018"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1060"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1135"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="1221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6217895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1316" mass="144993" checksum="A6CEC29B14C9BDC7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7F1</accession>
    <name>A0A8H6F7F1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147181.1">
        <property type="nucleotide sequence ID" value="XM_037297471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7F1"/>
    <dbReference type="GeneID" id="59334974"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="803" mass="92402" checksum="93B6343F1F447D18" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7G0</accession>
    <name>A0A8H6F7G0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oxaloacetate acetylhydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006797</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate</text>
            <dbReference type="Rhea" id="RHEA:16809"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57429"/>
            <dbReference type="EC" id="4.1.3.30"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate lyase/PEP mutase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146894.1">
        <property type="nucleotide sequence ID" value="XM_037297693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7G0"/>
    <dbReference type="GeneID" id="59335197"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046421">
        <property type="term" value="F:methylisocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000009">
        <property type="entry name" value="2-methylisocitrate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905:SF2">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13714">
        <property type="entry name" value="PEP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00061405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="46162" checksum="3247A339E3FCC01F" modified="2022-01-19" version="1">MAGATRTAIFSAAPELDREGQTLRSFPAEAAPKTKVDARHHIDGEVNGASSAETISNGDTAAHLQVNNTGNEASTAPISSTEKAIAENDVNDHREAPTAPNTGGDLPPQDMKTHHEPALVAESANGHVTATPNPLSPATKLKHRLRDTQDLIVCLGVYDGFSARIALSVGFDAMYMTGAGTTASRLGMADLGVASRTDMKEHADMIANLDPSKILIADMDTGYGGPITITTTVNQYIRANVAGFHIEDQLLTKRCGHLAGKKVVSQEVYLSRIRAAKAAKDKARSDIVLIARTDANQQHGYDECLTRLRAAQDLGADVGLLEGFKSKDEARRAVQDLAPWPLLLNVVENGHSPLITVDEARDMGFRIIIFSFGTLVPAYLAIKSAFERLKVEGITGVHETEISPRCIFDVCGLEEAMEVDENAGGEDFKGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7K8</accession>
    <name>A0A8H6F7K8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147361.1">
        <property type="nucleotide sequence ID" value="XM_037297236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7K8"/>
    <dbReference type="GeneID" id="59334739"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="233" mass="25152" checksum="11DBD04481DFE89C" modified="2022-01-19" version="1">MTTNTSPTFSTLGRSPSIGPPAYTTDDLLAARYQNFSRWVHLIRVAIAAITLVVSVAIIACAGASLRAFSNSRLEPEWILPLWPLNVDLRPSHAVLGCGIAVALLSLAYLAVAFIPMRQRLHNTNLVSTVLSFLCLCAALFTIVFASVVTNNLAYNTYSGTLNSWTCRWHGFDNVAPSNFTKICNDGMAALDLVIVLVIVEVLAVGATAWGWWVEMQLKKGGVGAAKSEVELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7Q4</accession>
    <name>A0A8H6F7Q4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147194.1">
        <property type="nucleotide sequence ID" value="XM_037297484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7Q4"/>
    <dbReference type="GeneID" id="59334987"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="286" mass="32341" checksum="C6F855A3531C2143" modified="2022-01-19" version="1">MTCYECSMIPYGAICTKCHQIQTSRAIALATPPAAAWPVIDRLGDWTGTKKSEEELLQNLDMELFELINVPHELDEWGRRVYNDCGHRILYYQDSDGNTVMGYEAWYPCSVTGEYEEPDDWRTGRQNRDSAVDKDAQYPEYDDCGRRTLCSAEPEGDTVTGGYEACYPNSVPDEYEEPENWRTGTQAYVPMIDFEQQRLAEFLAAQEEELPQFQSDRERIAAWIATSSVADDEDVSVLDCETKETVFQWYTGLEDFEPASPSQVGVPSCSDGTPEKGYVSVPLCAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7R7</accession>
    <name>A0A8H6F7R7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial large ribosomal subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005739</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147827.1">
        <property type="nucleotide sequence ID" value="XM_037296646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7R7"/>
    <dbReference type="GeneID" id="59334144"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000017">
        <property type="entry name" value="54S ribosomal protein L22, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047867">
        <property type="entry name" value="Ribosomal_uL22_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="CHLOROPLAST 50S RIBOSOMAL PROTEIN L22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL22M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="70"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="40196" checksum="4FE795FB9CAC83F2" modified="2022-01-19" version="1">MSLSNPRRRLLRSATSLLPHSHFHPLLACPHLNQRRTAITDFLKNPFRSRRRRKFLDLSSPEVLNANEIQKAKEEKERSKLPKTGGLAPGSILDTLSPTTGPRIEDTGVIRDPENMAAVLDPTPRAREKWQRRMVIREIRGRGRLTKTVKLARTERSHLSKSEFFKTSVKKLFPLANQIAGKPLSEAMVQMRFSKKKAAQDVLRHLEYARDQAVVMRGMGLGKVQAQAQQAEGGEVVDGREAERGQESAREEERLLVEDKKGKKRVVTDRTAMYVDQAWVGRGAYGWGVEYRARGTSNRLRLPYTSISVVLKEEATRIRQAEDRELKRQKKKVWVPLPDRPVTAQRQYPLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F829</accession>
    <name>A0A8H6F829_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147554.1">
        <property type="nucleotide sequence ID" value="XM_037296980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F829"/>
    <dbReference type="GeneID" id="59334482"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="75" mass="8063" checksum="712808AFA554C826" modified="2022-01-19" version="1">MSPISLLGRSAQIYADAAALGIDIPTNHSLFEGGELFDKTLLEDLRDQVYMLRGCSGEPVAHMPGFTPADLEAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8W4</accession>
    <name>A0A8H6F8W4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148756.1">
        <property type="nucleotide sequence ID" value="XM_037296058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8W4"/>
    <dbReference type="GeneID" id="59333552"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052432">
        <property type="entry name" value="PITP/CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46590:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46590">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CSR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="193"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="474" mass="53551" checksum="EB0DD88D255867A8" modified="2022-01-19" version="1">MPGAALPGRPGNLTKEQELRLQEMWTATLKVFGVSGFNNGTNGDAVSITGSETAEQAGTVGSEKKKKKRVNLFSRKHHDDVGTEDAESVSATDANDKYGQAKEFHKVLESQSPEAIRTAFWSMVKHDDPDGLLLRFLRARKWNVQNALIMLISTMHWRMQEMHVDDDIVRRGEAGALDDSINSTSPTKKEGHDFLTQMRLGKSFLHGTDKEGRPLTFVRVKLHKQGEQSEASLERFTVYTIETARLLLSPNVDTAAIIFDMTDFSMANLDYGPVKFMIKVFEANYPESLGVVLVHKSPWMFQGVWKLIRGWLDPVVAGKVHFTKNVEELAEFVERSHIIRELGGDDPWTYQYVEPVAGENKLQSDGVTRQRLLDERAAVVKDYEATTQQWIRDPNSTHALQQRRSELTNRLRTGYWELDPYLRARTLYDRTGIIREGGQIQYYGSPNSATGSNAGHHSIQNGPLPPEHRTDELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F987</accession>
    <name>A0A8H6F987_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148739.1">
        <property type="nucleotide sequence ID" value="XM_037296041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F987"/>
    <dbReference type="GeneID" id="59333535"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.390">
        <property type="entry name" value="Hemimethylated DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011722">
        <property type="entry name" value="Hemimethylated_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036623">
        <property type="entry name" value="Hemimethylated_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032698">
        <property type="entry name" value="SirB1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02097">
        <property type="entry name" value="yccV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350:SF27">
        <property type="entry name" value="HEMIMETHYLATED DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350">
        <property type="entry name" value="SI:DKEY-261L7.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13369">
        <property type="entry name" value="Transglut_core2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08755">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00992">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141255">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="2"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="581" mass="65811" checksum="B38FFEB369C5A544" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9J2</accession>
    <name>A0A8H6F9J2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149732.1">
        <property type="nucleotide sequence ID" value="XM_037294351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9J2"/>
    <dbReference type="GeneID" id="59331840"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14846" checksum="DC8154EBAA610B8D" modified="2022-01-19" version="1">MTELSNLNIIITDRLVLRPLRPTDRDAIRFLRFNPQVVAACLAHRKAETGAEFDAFLERVMEGGKCVSHCVELRVSSQSEGEKVEAKEQKLTQDEGNEPNSADKKHRENHIIGVMGAHRVPEIGYMFLPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9S7</accession>
    <name>A0A8H6F9S7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003729</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>propanoyl-CoA + oxaloacetate + H2O = (2S,3S)-2-methylcitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23780"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:58853"/>
            <dbReference type="EC" id="2.3.3.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the citrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149339.1">
        <property type="nucleotide sequence ID" value="XM_037294651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9S7"/>
    <dbReference type="GeneID" id="59332140"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050440">
        <property type="term" value="F:2-methylcitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06105">
        <property type="entry name" value="ScCit1-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010109">
        <property type="entry name" value="Citrate_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01793">
        <property type="entry name" value="cit_synth_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007128">
        <property type="entry name" value="PRK09569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF8">
        <property type="entry name" value="CITRATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR610109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="474" mass="52207" checksum="5A301B13910CD494" modified="2022-01-19" version="1">MATALRFSNAALRPALSSVRSPAKTAAFNGLRCYSSAKSQTLKESFAEKLPGEIEKIKKLRKEYGSKVIGEVTLDQVYGGARGIKSLVWEGSVLDSEEGIRFRGKTIPECQKLLPKAPGGEEPLPEGLFWLLLTGEVPSEEQVRDLSAEWAARSDVPKFIEELIDRCPNDLHPMAQFSLAVTALEHESAFAKAYAKGMPKSEYWSHTFEDSMDLIAKLPTIAARIYRNVYKDGKVPAVQKDKDYAYNFANQLGFSENKDFIELLRLYLTIHTDHEGGNVSAHTTHLVGSALSSPMLSLSAGLNGLAGPLHGLANQEVLNWLQKMKKQIGSDLSDKAITDYLWSTLKSGQVVPGYGHAVLRKTDPRYVSQREFALRHLPDDPMFKLVSQVYKIAPGVLTEHGKTKNPFPNVDAHSGVLLQYYGLTEASFYTVLFGVSRAIGVLPQLIIDRAVGAPIERPKSFSTEAWAKLVGATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA62</accession>
    <name>A0A8H6FA62_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002668</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150422.1">
        <property type="nucleotide sequence ID" value="XM_037293593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA62"/>
    <dbReference type="GeneID" id="59331080"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUZ" evidence="2">
        <location>
            <begin position="50"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="243" mass="27143" checksum="47836C52511959F9" modified="2022-01-19" version="1">MPDAWDDDWIAKGDSSPSKTEPAPISTKTSKAERRAKQAEFNRQLWEDAEDPQDNFFLKSRDIVPLKSEFRPAMKVLSRKPEKSSATNVEGIGQLNIDDDDESEDENKKHHLTAEERREKAQREREEKQKKYEEVRQKLFGNDQSSCATAASGPRNNTSKNSSPSRNQSRNKTSRDSRPSSSASNQTGRLYDPNDTGKPGVSSVLRKETKAEPNELQPIRSPRAPDNSGRGGFGFAPRGGKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAQ5</accession>
    <name>A0A8H6FAQ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">37S ribosomal protein mrp10, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150915.1">
        <property type="nucleotide sequence ID" value="XM_037293262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAQ5"/>
    <dbReference type="GeneID" id="59330749"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017264">
        <property type="entry name" value="Ribosomal_mS37_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15175" checksum="CD5B75393593726E" modified="2022-01-19" version="1">MMPTRRHIDECLSSPRSAALKTALRCINDNNSIPSVEPTTMPPKGGSTKLAPPRLPPLPKLRVRRPNRPGENPCVGIMTSVLGCWASSGNVAAHCQHLEQSLRACMDAPREKTGKKNTINYHLMRLYPKIKGPTKQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAS6</accession>
    <name>A0A8H6FAS6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">argininosuccinate lyase</fullName>
            <ecNumber evidence="4">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_002620</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150374.1">
        <property type="nucleotide sequence ID" value="XM_037293545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAS6"/>
    <dbReference type="GeneID" id="59331032"/>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000002">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000025">
        <property type="entry name" value="Argininosuccinate lyase chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="7">
        <location>
            <begin position="21"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="8">
        <location>
            <begin position="379"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="492" mass="55333" checksum="78D2558E6595F869" modified="2022-01-19" version="1">MAQSQEQAQEKTPGKNMLWGGRFTGGMDPLMNTFNASLPYDRILYAQDIAGSIAFARANKNRGILTSSEFEKIEEGFEKVKKEWEDNSFEVREADEDIHTANERRLCEIIGNEVGGKLHTGRSRNEQIATDMRLWLRDELRKIDQYLVDLLRVMTSRAEMEIELLMPGYTHLQRAQPVRWSHWLLSYGMAFAADLQRLRETIKRVNRSPLGCGALSGNPFGIDREAMAKELGFEGLMWNSMAAVADRDFALESIQWSSFLMSHVSRLAEDLIIHSTAEFGYVKLSDAYSTGSSLMPQKHNPDSLELLRGKSGRVFGQACGLQMSIKSTPSTYNKDLQESVEPMIDCVKTVGMSVQILTGVVSTMKLQKEKMWDALSPDMLAIELAEFLVRKGVPFRETHHISGRIVALAESEGVPMDKLSVQQIQSVDDRFDDDVKNCFNYERSVEMRSAKGGTSKASVLEQIAVLKDLLESKDAKLDAWGKQSKDDDGDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB44</accession>
    <name>A0A8H6FB44_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151382.1">
        <property type="nucleotide sequence ID" value="XM_037292843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB44"/>
    <dbReference type="GeneID" id="59330329"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="340" mass="36647" checksum="FEDF1DE144E6B774" modified="2022-01-19" version="1">MESKSEEREVTAGLLSNNRFAVLPPKEPAPGIQEERAFTIPLDPSPHHERPITLPLQTDIPPTGQSSSSEKIKDKSLAPTSLTTPRPNRPSIPRLAPHTPAKSTRIERNQQPSAPAAASISPTKPQAQLSPLVLQTEAQSGRSSRPPSASASARISPDFDTGDPYLSLLAPQPAPALPSPPIPQDPLPDPSLATLPIHPSLDFRFFTSPKSSGPKPPQLAPPIPTSIDIFRSDDPMRRIVRTRSGKIFCIAAEIAAANKKSTMRPVGHVLVPGTTKKVDEVKGGIDRKKSDCRSGEYVDSPEEMEDESEEEGGEGEEWLGKEEGFTQGSVLRENADSEMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB49</accession>
    <name>A0A8H6FB49_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151397.1">
        <property type="nucleotide sequence ID" value="XM_037292858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB49"/>
    <dbReference type="GeneID" id="59330344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018858">
        <property type="entry name" value="DUF2458"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10454">
        <property type="entry name" value="DUF2458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="302" mass="33554" checksum="3AA85B91B550FCE0" modified="2022-01-19" version="1">MESNYQPQTDLASILQTLRAYAPSQQQAPEATYQSQALRAHHQPQAPPQTPDLEEDEYDPSTYDPSLPLITVPHPQAAPAQAPDAQPQQSPPTPETRHRLEKTTPSYPRPPPEPTPAAKPPASTPGPKKAHAPPGHRPATITTWAPALRHVTGQTTADPEFGHRVRHLITTQRDHERQWWDGREDLKKRLAGREEGRKKLDSVLGSLGGHVNASKTDEFSEEEELKMYDRKVYQACREMVAATVKELKKLEVPFFCTVEGLVSPKGDGKAQGKRKGTVSEAELAELRGRMMVLLEDLCGEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBA1</accession>
    <name>A0A8H6FBA1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001749</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151216.1">
        <property type="nucleotide sequence ID" value="XM_037292677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBA1"/>
    <dbReference type="GeneID" id="59330163"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47815">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A FAMILY PROTEIN C25B2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47815:SF1">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A FAMILY PROTEIN C25B2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="79"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="381" mass="41783" checksum="F8D92449B2E15EDE" modified="2022-01-19" version="1">MSQEAITINNSTVQHIFKFHPEHPSASDYLENMMLNCFLDGSTYESRVSFDTFDNKDATDLSFTLVSKHKDYAYSRRSRTFLCGTDQNDYSEFALEWLVEELVEDGDEIVCLRVVDKDSKISSNFSVEQGFYKTEARKLLEHIQEKNEEDKAISLVLEFAVGKVPETIQRMIKIYAPACLIVGTRGKSLGGIQGLLPGSVSKYCLQNSPVPVVVVRPEEKREKKKKKRLADPSRQNYSSILDQTIASGSGAFESLARQSGGEASENEAAAVAKAIGLNEGSGAWKGFKKDDGDGSPLIKTVSGRSDATSEAESPSPTGPLVLDEALEDVPEIDDSEMSLLSDAGQKREEADDEEGAESKTFKEVDPINDSTFSGPEEAEAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBR7</accession>
    <name>A0A8H6FBR7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151938.1">
        <property type="nucleotide sequence ID" value="XM_037291576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBR7"/>
    <dbReference type="GeneID" id="59329056"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="247" mass="28310" checksum="A25E7AE362FE3286" modified="2022-01-19" version="1">MGVRNPTPASPFSEASASCTEATETPDKTEPLAEILDPEKTVATYGFVWHYDHNPVLVPQEITQKDWRSDSKELVQFYTIKETPPQSSDGSSAEIDDGDTYFSTHRAGVRLPRNNEKRVTFGCRFNESYKRLKGVKVIRRKSINADGTSWPFFEDSRRLEDLALRLRDEGDEKAAEFSEEEALRLVHRKTPEPYEIEETPEVDDVTEIEDDTDFSDLTNIEEGSEILETPELEDVWGFMVLFSDDMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCC6</accession>
    <name>A0A8H6FCC6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">40S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_000957</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152252.1">
        <property type="nucleotide sequence ID" value="XM_037291890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCC6"/>
    <dbReference type="GeneID" id="59329375"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000021">
        <property type="entry name" value="40S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="9">
        <location>
            <begin position="105"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="22305" checksum="73773E1C978D0600" modified="2022-01-19" version="1">MAPRSYSKTYKVPRRPFEAARLDSELKIVGEYGLRNKREVWRVMLTLSKIRRAARQLLTMEEKDPKRLFEGNALIRRLVRVGVLDESRMKLDYVLALKVEDFLERRLQTCVYKLGLAKSIHHARVLIRQRHIRVGKQIVNVPSFVVRLDSQKHIDFALTSPFGGGRPGRVRRKKARAAEKKDEGDAGEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCD3</accession>
    <name>A0A8H6FCD3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152069.1">
        <property type="nucleotide sequence ID" value="XM_037291707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCD3"/>
    <dbReference type="GeneID" id="59329188"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034253208" evidence="3">
        <location>
            <begin position="22"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="273" mass="24913" checksum="4622BD47B1967F4C" modified="2022-01-19" version="1">MRYSFALAALSAATAVMAGMANELSDGQVQEQVSAPPAPTPAAEATAFSTIVPFVSSPTPSPIEAGASSTYLVSPPVIATPSPSSPVVPVPGQAGATGSPKAPAPVVPVPVVPVPAVSSSPAVPAEVGASACPTCPSCTPVTIYSTVTATPTPAAAGSTGSSSSGSGSPSSGESGSSGSASSGSGSPSSGESGASGSASPSGKLPPSPGSPVSVPAGGAAGAAAPSGTAAASTGAVKPPSATGSPIAFKGAASTVGSSIAVAGLAAFAAIFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCD6</accession>
    <name>A0A8H6FCD6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000726</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152025.1">
        <property type="nucleotide sequence ID" value="XM_037291663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCD6"/>
    <dbReference type="GeneID" id="59329144"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048994">
        <property type="entry name" value="PH-GRAM_MTMR6-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF128">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,5-BISPHOSPHATE 3-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21098">
        <property type="entry name" value="PH-GRAM_MTMR6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="5">
        <location>
            <begin position="132"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="490"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="706"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="718"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="2">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="324"/>
            <end position="325"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="388"/>
            <end position="394"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="728" mass="81737" checksum="FCE386C86178114C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCM9</accession>
    <name>A0A8H6FCM9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)</fullName>
            <ecNumber evidence="3">4.3.3.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_000395</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by PDX2. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively. Also plays an indirect role in resistance to singlet oxygen-generating photosensitizers.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152769.1">
        <property type="nucleotide sequence ID" value="XM_037291334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCM9"/>
    <dbReference type="GeneID" id="59328814"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000001">
        <property type="entry name" value="Pyridoxine biosynthesis protein PDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00343">
        <property type="entry name" value="pyridoxal 5'-phosphate synthase lyase subunit PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="13">
        <location>
            <begin position="49"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="9">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="277"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR029271-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR029271-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6223552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="336" mass="35379" checksum="1CB12AD304822FCE" modified="2022-01-19" version="1">MAINDSPSTLVAPSTSTPTQDNAPTPASTSNRDNAPSPHTSTQDTSPNFTVKDGLAKMFKGGVIMDVVNADQARIAEEAGACAVMALERVPADIRAQGGVARMSDPQLVTEIMDAVTIPVMAKARIGHFVECQILQELGVDYIDESEVLTPADNAFHVEKHAFRTPFVCGCRGLGEALRRIDEGAAMIRTKGEAGTGDVVEAVRHMRTVTTEINKARGMSDMELRGYAKELNAPFRLLKETAQLGRLPVVNFAAGGVATPADAALMMQLGCDGVFVGSGIFKSGNAAKRAKAIVQAVTHFKDPAVLAEVSGGLGEAMVGLNVGRMAEGEKMAGRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCN2</accession>
    <name>A0A8H6FCN2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000303</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152677.1">
        <property type="nucleotide sequence ID" value="XM_037291242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCN2"/>
    <dbReference type="GeneID" id="59328722"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="108"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="172" mass="19349" checksum="5DD4FA581E5D0E5D" modified="2022-01-19" version="1">MTVALRSPQVVGGLIAVDNAPVDAILKSDFHKYVQGMRDIEQAQVSRQAEANDILKKYEEALPIRQFLLTNLVRSSDGELLRFRIPIKILASSLDKMGDFSFNNPDEVRYDGPTLIVRGTKSHYVADDVLPLIGRFFPMFKLVDIDSGHWVISEKPEAFRKAVVEFLEDAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCZ0</accession>
    <name>A0A8H6FCZ0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152525.1">
        <property type="nucleotide sequence ID" value="XM_037291090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCZ0"/>
    <dbReference type="GeneID" id="59328569"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="348" mass="36907" checksum="3BA9A95AF41992BF" modified="2022-01-19" version="1">MGTGIGTSPDYTVADSTKNGHHKMLKVLPTTQECHLYILRGDETVKDLSRSLRGCSSMAGKPELGRTSSLEAGMDCGINTSFRPCKVMYQCTEIVPQFAFPVNPNSGFFNMRSSTPSPLAAFMLFASSVLCQNPTTTTINLGCYTSTGITSQKSVPVVVYTSTVTVTTALPKITVTKVTSTSTATTISTITPSIASTATWTITPPPVTVTSTSLVTALGATTTTTITPGWDRLKRHPAPTPAAPLFPRAHAIDRRGGFPQTVYCGAEVYVRTTKTITSVTHTAVSYATTTSTFTATINEVVSTQTITPAISTISSTVTVASTVVSTCFEGEKTTVCIHILNLYPSRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDF6</accession>
    <name>A0A8H6FDF6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153345.1">
        <property type="nucleotide sequence ID" value="XM_037301711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDF6"/>
    <dbReference type="GeneID" id="59339250"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="72" mass="7751" checksum="C03A3ED2DC928E18" modified="2022-01-19" version="1">MNGFSWGDDGAGEEGSADDDDDDAGRENRMEQVAVGMGLSLGSVFQKADEEGNMTGCDCFDIAARWFEDCFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE02</accession>
    <name>A0A8H6FE02_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF292-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IST1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153449.1">
        <property type="nucleotide sequence ID" value="XM_037301815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE02"/>
    <dbReference type="GeneID" id="59339356"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein IST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF5">
        <property type="entry name" value="IST1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="33180" checksum="439D9BA96A5DCBB8" modified="2022-01-19" version="1">MAPSSAIVGKIKVQLKLSISRLRMVQQKDTAIAKQQRRAMAVLLEQGKEESARIRVENIIRSDITTELLEVLELYCELLLARTGLLEAKECDPGLEEAIKSIIYAAPRTDVKELQQARALLVEKYGKDFALEAVENSDGKVAERVLKKLRVEPPEPELVTLYLKEIARTYGVNWPRKEESLDAQDDGDDDKPSGGQAVKNLEEPLTTDELSKATPPRDLGPRSPISVAPPSPSTDNLNPRVRLPGPPELKPGAKMSGLKKQTSDTDAQSTATSRESGAKKDIVGGKIPDVDELTKRFAALKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE55</accession>
    <name>A0A8H6FE55_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010369</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="4">The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154039.1">
        <property type="nucleotide sequence ID" value="XM_037301222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE55"/>
    <dbReference type="GeneID" id="59338760"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004552">
        <property type="entry name" value="AGP_acyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00530">
        <property type="entry name" value="AGP_acyltrn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434:SF11">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="228" mass="24775" checksum="91BF842A1C3EEA3D" modified="2022-01-19" version="1">MALTTGIRFEIVSGGEYLNTRPCVLIGNHQSELDVLLLGTIFPPYCSVTAKRSLRLVPFLGWFMALSGTVFIDRSNSASAHAAFDKAAAEMREERQSVFIFPEGTRSYAEGPEMGAFKKGAFHLAVKAGVDIVPVVAGNYWGVLSVKEQRFRSGRIPVKVLPPIPTAHLTAADVEELTRTTREKMLKELVALTESPMGQKATKAHVEAGEEDLARLATPTAMASGLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FET4</accession>
    <name>A0A8H6FET4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_009711</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154419.1">
        <property type="nucleotide sequence ID" value="XM_037300571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FET4"/>
    <dbReference type="GeneID" id="59338106"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17954">
        <property type="entry name" value="DEADc_DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044765">
        <property type="entry name" value="DDX47/Rrp3_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF20">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="15">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="102"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="302"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="464"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="10">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6225710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="54039" checksum="264188CD76112201" modified="2022-01-19" version="1">MSPIQKRKADGSSHKDEGIPSKRVKHTTSTHTKQTAPEPAKESSPQPSSEPSLVTGSTSEVEIEAKGHASKSFQDLGIIQSLCDACTALGYEAPTPIQTEAIPLALQGRDIIGLAETGSGKTAAFALPILQALMESPQSSLFGLCLAPTRELAYQIAQAFEALGALISVKVAVVVGGMDMISQSIALGKKPHIVVATPGRLLDHLENTKGFSLRSIKHLVMDEADRLLDLDFGAIITKILKVLPRERRTCLFSATMTSKVESLQRASLSNPLRVSISTNKYQTVSTLLQSYCFIPHKDKDVYVAWLMNEFASKSVIIFTRTVNETQRIAILLRALGFGAIPLHGQLSQSSRLGALGKFRARSRDILVATDVAARGLDIPSVDVVLNFDLPHNSETYIHRVGRTARAGKSGHAISMVTQYDIEVWQKIEAALGKKLGEYETDRAEVMILAERVGEAQRMAIREMKDLHEKRGTKGATLRGRKPGGGKKGRNDMDREEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEY5</accession>
    <name>A0A8H6FEY5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009862</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154569.1">
        <property type="nucleotide sequence ID" value="XM_037300721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEY5"/>
    <dbReference type="GeneID" id="59338257"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="160" mass="17908" checksum="8304E4764CF03BCC" modified="2022-01-19" version="1">MSWFSILPANLTVIETWIIRFFLLLGIMTIGPWALFLIYDVLLYIIRAVAYEIPYFGGRARGRGRPRAPSLKERPNGRRRTFSISGPSSGFVVGDENDKAGLKGRGLDGGVRDEAAANSEDDETVDITAEESHMIRSADLWDARTDSRFVAWNDTSKKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFG7</accession>
    <name>A0A8H6FFG7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009057</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154744.1">
        <property type="nucleotide sequence ID" value="XM_037299918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFG7"/>
    <dbReference type="GeneID" id="59337452"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="165"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="336"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="369"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="402"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="555" mass="60795" checksum="CBF15FD3E6696BD5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGG6</accession>
    <name>A0A8H6FGG6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008668</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155534.1">
        <property type="nucleotide sequence ID" value="XM_037299533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGG6"/>
    <dbReference type="GeneID" id="59337063"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6227226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="156" mass="16853" checksum="F3A841E8C91978FD" modified="2022-01-19" version="1">MAAAISALNAKIRSQPVLNYVCSTHFWGPVSNFGIPIAAVMDTQKDPDIISGRMTGALVLYSGTFMRYALAVQPKNYLLFGCHFVNFGAQLTQGYRYLEYWNYGGREASLEAKAKTEANQIAQDGKSIAGDAKAGAEGIVGDVKLKAGELKQKVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGL0</accession>
    <name>A0A8H6FGL0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with coenzyme Q.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COQ10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155397.1">
        <property type="nucleotide sequence ID" value="XM_037299396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGL0"/>
    <dbReference type="GeneID" id="59336926"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901:SF10">
        <property type="entry name" value="COENZYME Q-BINDING PROTEIN COQ10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="SPERM PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="5">
        <location>
            <begin position="62"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="253"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="257"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6227089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="282" mass="30898" checksum="2BA356A3B01328F8" modified="2022-01-19" version="1">MKATRRLLLLRPSPALHPHPASLLSKPRPTPTHPQPPSRPSRPFVSNPLTPLQTLTASRILPYHAAPLYELIADIAAYPDFIPYCTSSTITSLSAPDAAHRKEWPRTADLRIGYGPYDELFRSAVYCLPHTVLEAVAGDAEPTIPKSRLAHYYEDPGHEDDAEKSAGNRSIFTSLLTRWTFREFPFKPAPPDGKTPQEGSASAPSRPRTEVNLVLEVRFASAVYSALSQAAAPKVAGMMIEAFEKRATVVLGQGHGPESRAEEDGSKRSAMEGVTRGKNRIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHP9</accession>
    <name>A0A8H6FHP9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008018</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156222.1">
        <property type="nucleotide sequence ID" value="XM_037298886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHP9"/>
    <dbReference type="GeneID" id="59336415"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13981">
        <property type="entry name" value="STKc_Bub1_BubR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.430:FF:000003">
        <property type="entry name" value="Checkpoint serine/threonine-protein kinase BUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015661">
        <property type="entry name" value="Bub1/Mad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013212">
        <property type="entry name" value="Mad3/Bub1_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012572">
        <property type="entry name" value="Mad3/Bub1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030:SF4">
        <property type="entry name" value="BUB1 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030">
        <property type="entry name" value="MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08311">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08171">
        <property type="entry name" value="Mad3_BUB1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00777">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51489">
        <property type="entry name" value="BUB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0137">Centromere</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BUB1 N-terminal" evidence="7">
        <location>
            <begin position="59"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="850"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="520"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="532"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="553"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1189" mass="131596" checksum="7C76F74322D9D902" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI43</accession>
    <name>A0A8H6FI43_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbonic anhydrase</fullName>
            <ecNumber evidence="5">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007048</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156578.1">
        <property type="nucleotide sequence ID" value="XM_037297943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI43"/>
    <dbReference type="GeneID" id="59335448"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03379">
        <property type="entry name" value="beta_CA_cladeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175:SF3">
        <property type="entry name" value="CARBON DISULFIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="241"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="278" mass="31196" checksum="47FD1B43613EB92B" modified="2022-01-19" version="1">MAEEKPEDSRVANFLSRNATFAETYKPAPLFAKLLANPPVHPRITIISCFDGRVDPRKFFGLQPGDAVLISNAGGRVNADALRSIIILDDLLVVGTVIVVHHTDCGLVHTSDESIRARLKGRAPEKAQEIEGMEFELFKDVHESVRHDMGIVKASPFLDVQVFGYIYDVTHGTVEERLSKDFHDQLGIDTICAWGSMEPNVKVALRRQLKELCFQKGLFTGRDVEPALEWRLYQLQRNRRAEARKVKPTTESVSESSPTANTQDSTTKVSYYDPVRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI99</accession>
    <name>A0A8H6FI99_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156687.1">
        <property type="nucleotide sequence ID" value="XM_037298052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI99"/>
    <dbReference type="GeneID" id="59335559"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="240" mass="26112" checksum="F401AC6AB32859F4" modified="2022-01-19" version="1">MESKPANTFTGAAKLLPQQLVLPDAAEALKRAKIERFLRGTATFEQEQEAKAHASAAAPYFNLPARGQSMCAYELPVGLGHISGSLKIDDPPTPMRIGNLSTFIQKTDPLTGIKYWVGLTWAEIFFPVLGERPSSVAGSKAYEVVLKQPIEDGGHIVRENVVTTGRVVGVVVAELPHETVSVRIQDWFSKSDMFKKGRWNQLDIQARDGAGADLAVTHGALRGGNNEENSVRASCDETQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIG0</accession>
    <name>A0A8H6FIG0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoserine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156775.1">
        <property type="nucleotide sequence ID" value="XM_037298140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIG0"/>
    <dbReference type="GeneID" id="59335647"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050849">
        <property type="entry name" value="HAD-like_hydrolase_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181:SF2">
        <property type="entry name" value="PHOSPHORIC MONOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181">
        <property type="entry name" value="UPF0655 PROTEIN YCR015C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12710">
        <property type="entry name" value="HAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22222" checksum="155FE7EBEC7FC3B1" modified="2022-01-19" version="1">MDSVKTPFPDCIKALCDNIKLDPYFNDFYKWSRENGVPVIVLSSGMTPIIRALLVHLIGPEGNDIEIVSNEVADRPPKKMAEEAGWQIKFHDESGFGHDKSLTIRPYAKYFEDNPNEPRPTMFYAGDGVSDLSAARETDLLFAKKGHDLITYCEREGVPFTVFEDWQSILATTKAIFEGKTSVKELAAESAEKAKEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIG5</accession>
    <name>A0A8H6FIG5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156792.1">
        <property type="nucleotide sequence ID" value="XM_037298157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIG5"/>
    <dbReference type="GeneID" id="59335664"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="137" mass="14873" checksum="44BEF75828497A38" modified="2022-01-19" version="1">MAKGTVGQWRVGEYERSEELQVAVNGMTAPLLSARNRDGSVLTTYANDDSGLERHKRLNKAYIKELENRGLLDDEDPHTIQESSEVAGTDNAYPASSSSESEILPEAALFAPSKTESEMSEDDLPRATGNGLNTATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FII5</accession>
    <name>A0A8H6FII5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156803.1">
        <property type="nucleotide sequence ID" value="XM_037298168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FII5"/>
    <dbReference type="GeneID" id="59335676"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="13377" checksum="B87913777E7EF2FD" modified="2022-01-19" version="1">MNTSTPPTGSTAAALPDATYVPEPHQVPIGIWSGVEDEQYTKQPDEPMAEFDAEVPIHSGKDSEEALMVLTKRRAEIRDARSVSSVRLLFTVYELVFRNGGNEESNVGKKTRKCERSPSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK12</accession>
    <name>A0A8H6FK12_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Meiotically up-regulated protein Msb1/Mug8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157246.1">
        <property type="nucleotide sequence ID" value="XM_037295245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK12"/>
    <dbReference type="GeneID" id="59332735"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04401">
        <property type="entry name" value="RhoGAP_fMSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037508">
        <property type="entry name" value="Msb1/Mug8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012965">
        <property type="entry name" value="Msb1/Mug8_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093:SF1">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08101">
        <property type="entry name" value="Msb1-Mug8_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meiotically up-regulated protein Msb1/Mug8" evidence="2">
        <location>
            <begin position="53"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="678"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="823"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="895"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="596"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="753"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="772"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="826"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="901"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="949"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1022"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1065"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1087"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1124" mass="121618" checksum="20B5DD90CE5B1A17" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKT4</accession>
    <name>A0A8H6FKT4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157619.1">
        <property type="nucleotide sequence ID" value="XM_037295618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKT4"/>
    <dbReference type="GeneID" id="59333110"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051414">
        <property type="entry name" value="Adenylate-forming_Reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439:SF2">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439">
        <property type="entry name" value="PHENYLACETATE-COENZYME A LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="320" mass="34931" checksum="A822532FFC8BB3AB" modified="2022-01-19" version="1">MAQTRPSAIYAEVPRSSDSYEDGYRKVTYGALANAVNGVAWSLKGMLGEGQNHQTLAYNGPNELGICDDDLRTTTLSSIEHPTYDNGQAGNLFATLFDAIANQTTVITPLAAVTPSARSVVDGLKHVKADALILAPPFLEQIAKSPDMVVFIASNIETVTSVGASPLKKDLWAYRGRADDTILFKQGYMCNQISMEQHVSQHSEVRAVLMAGTGRSQPALIIERAGDQPLSSPAEQELTERIWPIIEEANRTYKMGTRVSKSHILDTNLLQPMRRAGKGTVQRCPTLELYKDALDALYAREGDIVPGNELVLPSFNPKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKT9</accession>
    <name>A0A8H6FKT9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157636.1">
        <property type="nucleotide sequence ID" value="XM_037295635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKT9"/>
    <dbReference type="GeneID" id="59333127"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF15">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="2">
        <location>
            <begin position="131"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="495" mass="55608" checksum="1187AB11E5C37467" modified="2022-01-19" version="1">MICSSHFCAEHSRLPFHYPSQDTESDAYWTAYHSANTKHLLNRLYQVKTTALVSLASRLRNGISCTIPALMPDGQGHLDVDAVSRQMGGQNCNIDVQFEDGVVWLARIRLDDPLLPPKPTQAYIFLSEVFTLKYLEDTGIPAPKVFHFATESSENPVGVPFMLIEKMKGAPLMWDTTTPIQRTKVLEQLADILLALEKHSFHSTGSLFPSNGSGNVCGFAQSQLFDSPNTTLGPFDTLESSLRAMLAHQRRLIANGELSSLAIDHYLSHRWREDMVPEVLSLHNDAGFFLKHFDDKGDHILVDDDFNITGIIDWEFASLEPKAQAFSSPCMLWPVGDFHAGSNRLSPEEMEFAAIFERRGRNDMAHLVRNGRKMQRYLFFNGGSVSHEQEDFEAMFQGLRAAWAGDNSQPGPYQVWKEEALKKYEGDEQLGRLLQRNSTRSQSATHLDCGPSTPQRHHSLKDESSEILDTGMREGPAGFCMRVAARLSKNKDIAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL72</accession>
    <name>A0A8H6FL72_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157789.1">
        <property type="nucleotide sequence ID" value="XM_037295788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL72"/>
    <dbReference type="GeneID" id="59333282"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="817"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="836" mass="82544" checksum="843EBCCDC9C2E6A8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYS5</accession>
    <name>A0A8H6KYS5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sulfite reductase [NADPH] subunit beta</fullName>
            <ecNumber evidence="6">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_007421</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the reduction of sulfite to sulfide, one of several activities required for the biosynthesis of L-cysteine from sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Alpha(2)-beta(2). The alpha component is a flavoprotein, the beta component is a hemoprotein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156947.1">
        <property type="nucleotide sequence ID" value="XM_037298312.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59335820"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050311">
        <property type="term" value="F:sulfite reductase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000007">
        <property type="entry name" value="Pyruvate:ferredoxin (Flavodoxin) oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000003">
        <property type="entry name" value="Sulfite reductase [NADPH] hemoprotein beta-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000004">
        <property type="entry name" value="Sulfite reductase [NADPH] hemoprotein beta-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000085">
        <property type="entry name" value="Sulfite reductase [NADPH] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000051">
        <property type="entry name" value="Sulfite reductase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000016">
        <property type="entry name" value="Sulfite reductase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01540">
        <property type="entry name" value="CysI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011786">
        <property type="entry name" value="CysI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010029">
        <property type="entry name" value="PRK13504.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493:SF47">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="22">
        <location>
            <begin position="791"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="247"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00057613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00063391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00067595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAF6229305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1531" mass="169913" checksum="6BA0499A17D5D16A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C685</accession>
    <name>A0A8H6C685_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006676</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147009.1">
        <property type="nucleotide sequence ID" value="XM_037297573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C685"/>
    <dbReference type="GeneID" id="59335077"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051571">
        <property type="entry name" value="N-CoR_corepressor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992">
        <property type="entry name" value="NUCLEAR RECEPTOR CO-REPRESSOR RELATED NCOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992:SF39">
        <property type="entry name" value="SMRTER, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="783"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="345"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="794"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="851" mass="94376" checksum="178A5C15DA0C0CA1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6D7</accession>
    <name>A0A8H6C6D7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial</fullName>
            <ecNumber evidence="5 18">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HO133_006839</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="18">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="18">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 18">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion inner membrane</location>
            <topology evidence="1 18">Peripheral membrane protein</topology>
            <orientation evidence="1 18">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="5 18"/>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146936.1">
        <property type="nucleotide sequence ID" value="XM_037297735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6D7"/>
    <dbReference type="GeneID" id="59335239"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit SdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13534">
        <property type="entry name" value="Fer4_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="16 18" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8 18" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 18" id="KW-0408">Iron</keyword>
    <keyword evidence="15 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="20">
        <location>
            <begin position="82"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="21">
        <location>
            <begin position="203"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF6217501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="300" mass="34081" checksum="9969E3F0F52F3529" modified="2022-01-19" version="1">MAVLRTTTSRILASTRPSTLSPFLRRTYATVEPISNLGEAMSATQRPVSPKHTSTIHEPAQSPEARTKTFHIYRWSPDTPTEKPKMQSYTLDLNKTGPMVLDALIRIKNEVDPTLTFRRSCREGICGSCAMNIDGVNTLACLCRIPANTSHEARIYPLPHTYVVKDLVPDLTQFYKQYKSIKPYLQRDTKTEDGLENRQSPEDRKKLDGLYECILCACCSTSCPSYWWNSEEYLGPAVLLQSYRWLADSRDEHKAERKEMLDNSMSLYRCHTILNCSRTCPKGLNPGKAIAEIKKTMAFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Q7</accession>
    <name>A0A8H6C6Q7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147116.1">
        <property type="nucleotide sequence ID" value="XM_037297680.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59335184"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="186" mass="20998" checksum="A4823C4D563C69A3" modified="2022-01-19" version="1">MASERYLKFLLSDEYDPAGDSPYKFRQDYDRLAEHRDLLRNQSNAKEQTRATKRQAEGQCPGGASAAASDMQGTRFSVLPDNDNGSTISPSTKQNFSANFTIAQLKSLTKDQRKAEVNKVKRDIQALKKEKKAKKDAAAAEAKEKKVREEAKNEEKQEEAKKEDQDEASELDLGQDWEKVNRAGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7H4</accession>
    <name>A0A8H6C7H4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6604 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147714.1">
        <property type="nucleotide sequence ID" value="XM_037296533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7H4"/>
    <dbReference type="GeneID" id="59334030"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046539">
        <property type="entry name" value="DUF6604"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20253">
        <property type="entry name" value="DUF6604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6604" evidence="2">
        <location>
            <begin position="88"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="342" mass="38826" checksum="ACD26EB1E821C35D" modified="2022-01-19" version="1">MLAIAASRLSPSDTWKRKNEKTKERPILGSWRERRGSKESGEEKGSGETKETLGSWRLRKRERTKAMDETGVHGDCIPAEWKLQKMYKQLKIDNNKFTTWLVESVYPCGRSLSCSETLPPCITLSRKQAKGMSKPDWAVTSNAILTTSTDHPKYDIIVNNITELARQTQKTPASMMALLDRLIVERTEYSYWMSRKSTDDLSKFSNESHVYYIEVLKEVKVILAEHIEPQTTRLCQAESLTLSETTNIPTQTPNPTLGKRKVSSTSHQNPDRRKAMKTESTTSSPKHVLQKEAKHLTTTQRQQEKEWRHVVANSCNKMAEKDCAPVAKPMSYAAALRVAVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C840</accession>
    <name>A0A8H6C840_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005945</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148029.1">
        <property type="nucleotide sequence ID" value="XM_037296848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C840"/>
    <dbReference type="GeneID" id="59334350"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF162">
        <property type="entry name" value="SATRATOXIN BIOSYNTHESIS SC1 CLUSTER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="36"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="332" mass="37206" checksum="3F244DDDA11D4B73" modified="2022-01-19" version="1">MAPSTTHSPAKTLSRSEYLAVSWTFTSFAILLSFGRFVIRKRLLGKLQMDDWSHAFALVLLIPYMALSTTIYPLAIEVGLFGKKDGPMPSKERLERFFRLEVAGQLFFWIILYAVKFTFLILFRQIFSVNKIFMRWWWAVFIYTCIAFWACFLEVFWICGSPSDIFVLKKCLTPHASGVVNNFAEVGLALNISSDLAVMILPTCFLRGLQMQLGKKIAVGLLFSVGMVVVVIDAVRLAVGDGGGVVSQALLYDALEPAIAVIMSCLPTYRTLISPARGSSRNLKGDRYQYESGSQQSWKDRITARYRSEGYELSNDSEALTRPPATLPLGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C854</accession>
    <name>A0A8H6C854_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005272</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148165.1">
        <property type="nucleotide sequence ID" value="XM_037296183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C854"/>
    <dbReference type="GeneID" id="59333678"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034048839" evidence="2">
        <location>
            <begin position="21"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="343" mass="35433" checksum="DC33D1324FDF51D9" modified="2022-01-19" version="1">MSLILIVLLQLSLLLRNVISTCSTTSDVEFTFYGFPDGPSDTTSFGCSGTTQVADGTAGGDGSYGNPETFATALSNTNFVPCEIIYIPLFQKYFQYMDHCEECNVLYPETTRIDLWIGTDVNGGPKQIACEDAYGLKTGQTIVHDPPSTLQVNGGQLWDNDSGACNDDSLVFPNYSTSEADMCAGGSTSSSGGSPAGSPAPSSTSRTSAPPASTPSTPAISPNSGAPSMGSGDASPKKEKLDTVPNVDTVSKVADVKVNKPSPTTFATVVSATPSTSTSSVPSPSGSASGSPSSTDCGVTAEWPTAWLGHCVGTPCHDFNDCSGELICQGWPNPVCTNPANRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8I1</accession>
    <name>A0A8H6C8I1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2Fe-2S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005405</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148297.1">
        <property type="nucleotide sequence ID" value="XM_037296315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8I1"/>
    <dbReference type="GeneID" id="59333811"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="7">
        <location>
            <begin position="14"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="131" mass="14334" checksum="330672A2C4CD2867" modified="2022-01-19" version="1">MHGHLTPPKPGEELNVTFVDKEGEEHSFQVAHGDNLLDIAQSEELEMEGACGGSCACSTCHVIVESDEMYEKMDEPDDDENDMLDLAFGLTETSRLGCQVKMNKALNGLRVKLPSMTRNLQASDFSGAKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8M5</accession>
    <name>A0A8H6C8M5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148377.1">
        <property type="nucleotide sequence ID" value="XM_037296395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8M5"/>
    <dbReference type="GeneID" id="59333891"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26113">
        <property type="entry name" value="GH16_XgeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="261" mass="28983" checksum="981230291858EFA0" modified="2022-01-19" version="1">MLTPQNIQQIIAGSKPFLRWYVRTFNNINVVSSTASGWTLKSRYKEEEEEEEEEEEEEEEEEEDNTREQLSLTASNNFTGDDPSHGFIHYVNQQGSVEQKLTFASANSVILPLIQLMRMPVRAVIRSKSPENRHSIQACSYSTFFTHQTAVGLGKQKKKSTRGYRCEDVRRPAIWTADPSSWPKTGGIDVLEAVNSATTGNQTTLHTSNGCTVHAKRKELGKVLTRDCYNGTDSNAGCGVQGPDDTFGEALNNNCGGVSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8P1</accession>
    <name>A0A8H6C8P1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005496</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148387.1">
        <property type="nucleotide sequence ID" value="XM_037296405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8P1"/>
    <dbReference type="GeneID" id="59333902"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140107">
        <property type="term" value="F:high-affinity potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004773">
        <property type="entry name" value="K/Na_transp_Trk1/HKT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015958">
        <property type="entry name" value="Trk1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00934">
        <property type="entry name" value="2a38euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF30">
        <property type="entry name" value="HIGH-AFFINITY POTASSIUM TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002450">
        <property type="entry name" value="K+_transpter_TRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="484"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="546"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="669"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="698"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="248"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="771"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="813"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR002450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6218952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="868" mass="96840" checksum="6F13E2200D417C58" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Q6</accession>
    <name>A0A8H6C8Q6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005511</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IMPACT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148402.1">
        <property type="nucleotide sequence ID" value="XM_037296420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8Q6"/>
    <dbReference type="GeneID" id="59333917"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23822">
        <property type="entry name" value="RWD_ScYIH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020569">
        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF25">
        <property type="entry name" value="PROTEIN IMPACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RWD" evidence="7">
        <location>
            <begin position="7"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="277" mass="29962" checksum="9DE6A436080BAE8F" modified="2022-01-19" version="1">MSEDLLDEIEAIQSIYGNDVLREADGAFVYILSIPHVEASLRVLFPSDYPESIPQLLGIERTGAQARKGYGSHVLDTAQATLLSVFISGSVCLFDLLQELDTVLAEDSNGQQSSLADPEDDARLVLKAAPPVTCGLGEEPRWILSSAVTEKKSTFVARACSVTSPAQAQAFLAHLLVNDKRTAKASHNPTAYRIRAPSTAKSASEVLYEDCDDDGENAAGGRLLQLLKIMDVWDVLVVVSRWYGGVKLGPDRFSIINNVAREAVVEGGWTKSGIRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8S2</accession>
    <name>A0A8H6C8S2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear condensin complex subunit 3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005494</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CND3 (condensin subunit 3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148385.1">
        <property type="nucleotide sequence ID" value="XM_037296403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8S2"/>
    <dbReference type="GeneID" id="59333900"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027165">
        <property type="entry name" value="CND3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025977">
        <property type="entry name" value="Cnd3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418:SF5">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12719">
        <property type="entry name" value="Cnd3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear condensin complex subunit 3 C-terminal" evidence="9">
        <location>
            <begin position="684"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="966"/>
            <end position="988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6218950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1034" mass="114909" checksum="A8B948D248610CF6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8U3</accession>
    <name>A0A8H6C8U3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE affecting a late Golgi compartment protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_005513</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148404.1">
        <property type="nucleotide sequence ID" value="XM_037296422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8U3"/>
    <dbReference type="GeneID" id="59333919"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21444">
        <property type="entry name" value="SNARE_NTD_Tlg1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15851">
        <property type="entry name" value="SNARE_Syntaxin6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000012">
        <property type="entry name" value="SNARE domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000006">
        <property type="entry name" value="Syntaxin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015260">
        <property type="entry name" value="Syntaxin-6/10/61_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048036">
        <property type="entry name" value="Tlg1p-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09177">
        <property type="entry name" value="STX6_10_61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="165"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6218969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="246" mass="27007" checksum="363EBCB92DEB606C" modified="2022-01-19" version="1">MQETLNGVGSGVGQSQAINSQKLTILLSDVLSTLKTTRSLFSSYLRIRSLASSASSPELAQARSELESTVQDLSTDLQDLVDSVKAVEGDPFRYGLDIDEVGRRRKLVQDAGGEVESMRQELAKTVIGAQSKSQGMNGTLPPPSAFDPDEPDGDDYGVFEQQRQVEMMHEQDEALDGVFKTVGNLRQQADEMGRELEEQGEMLEDADRLADRVGGKLQNGIKRVGWVIKNNEGMRTFDQSPPRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9A0</accession>
    <name>A0A8H6C9A0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148481.1">
        <property type="nucleotide sequence ID" value="XM_037296499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9A0"/>
    <dbReference type="GeneID" id="59333996"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="168" mass="18984" checksum="58929213C3363E3C" modified="2022-01-19" version="1">MEVLNELFPKMGLECRGSIVFIHLACCDGDPNSFEVEHLKSAEPQDRLCSGVVHLWSGLVQRERGGELEYSDPTTMSYRVASAPYVHRIMEHPGIRIVLYRDQDPESVEQTVRPGNWREVGRDTIEAEEHRDRMISRIGPHLYVFEPIAESTAAVPVAGPSAKMQIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9B1</accession>
    <name>A0A8H6C9B1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005510</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148401.1">
        <property type="nucleotide sequence ID" value="XM_037296419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9B1"/>
    <dbReference type="GeneID" id="59333916"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22572">
        <property type="entry name" value="GCP5_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059169">
        <property type="entry name" value="GCP5_N_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032797">
        <property type="entry name" value="Mod21_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF33">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14609">
        <property type="entry name" value="GCP5-Mod21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-Tubulin ring complex non-core subunit mod21 N-terminal" evidence="7">
        <location>
            <begin position="64"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="232"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="6">
        <location>
            <begin position="624"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="888" mass="100403" checksum="6B40A75268AAB7D1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9H7</accession>
    <name>A0A8H6C9H7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005154</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148764.1">
        <property type="nucleotide sequence ID" value="XM_037296066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9H7"/>
    <dbReference type="GeneID" id="59333560"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13311">
        <property type="entry name" value="PH_Slm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043453">
        <property type="entry name" value="Slm1_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF16">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE-BINDING PROTEIN SLM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="386"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="504"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="665"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="732"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="782"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="571"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="732"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="784"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="845" mass="90965" checksum="D776120BB2BE33C4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9J8</accession>
    <name>A0A8H6C9J8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aldehyde dehydrogenase (NAD(+))</fullName>
            <ecNumber evidence="3">1.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004009</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148975.1">
        <property type="nucleotide sequence ID" value="XM_037294928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9J8"/>
    <dbReference type="GeneID" id="59332418"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07098">
        <property type="entry name" value="ALDH_F15-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000024">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="89"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="613" mass="67233" checksum="90AF56A07B64D1B0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9K9</accession>
    <name>A0A8H6C9K9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2-methylcitrate dehydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003934</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148902.1">
        <property type="nucleotide sequence ID" value="XM_037294855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9K9"/>
    <dbReference type="GeneID" id="59332344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047547">
        <property type="term" value="F:2-methylcitrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019679">
        <property type="term" value="P:propionate metabolic process, methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4100.10:FF:000001">
        <property type="entry name" value="2-methylcitrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.120:FF:000001">
        <property type="entry name" value="2-methylcitrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4100.10">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.120">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012705">
        <property type="entry name" value="2Me_IsoCit_deHydtase_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042183">
        <property type="entry name" value="MmgE/PrpD_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042188">
        <property type="entry name" value="MmgE/PrpD_sf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045336">
        <property type="entry name" value="MmgE_PrpD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006943">
        <property type="entry name" value="PRK09425.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02330">
        <property type="entry name" value="prpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943:SF16">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03972">
        <property type="entry name" value="MmgE_PrpD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MmgE/PrpD N-terminal" evidence="4">
        <location>
            <begin position="23"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="MmgE/PrpD C-terminal" evidence="5">
        <location>
            <begin position="296"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="55998" checksum="B44D806F12E84165" modified="2022-01-19" version="1">MAALKSNAPAMAGVREFDPEIKDMASYIHNYRVDSDLAYETARYVFLDTIGCGLEALRFKECTKLLGPIVEGTIVPHGTRVPGTPYVLDPVNGAFNIGAMIRWLDYNDCWLAAEWGHPSDNLGGILAVADWISRTNRAGGHVGNGTIVKIKDVLEAMIKAHEIQGCLALENSYNKVGLDHVVLVKVATAAVVSKMLGLPERKTADAISQAWVDGQSLRTYRHSPNTMSRKSWAAGDACQRAVNLVLKVQKGEHGVPTVLSAPVWGFYDVAFKGKKFEFQRPYGSYVMENILFKVSYPAEFHSQTAIEAAKKINAQLSEQGKSAEDIKSITCRTHEACIRIIDKQHKPMDNFADRDHCIQYMVATMLVFNRLHATDYTDGSEAATSPLVEELRRNISCVEDPEFTRAYHDPNMRTIPNALTVTLEDGTVLEETVVEAPLGHKLRREEAKPEILEKFERHLGPHFESEKVQELVELGKNKERLENMGVDEYMDKYVKEKMEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Q3</accession>
    <name>A0A8H6C9Q3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ethanolamine utilization protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003957</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148925.1">
        <property type="nucleotide sequence ID" value="XM_037294878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Q3"/>
    <dbReference type="GeneID" id="59332367"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02228">
        <property type="entry name" value="cupin_EutQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010424">
        <property type="entry name" value="EutQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36169:SF1">
        <property type="entry name" value="ACETATE KINASE EUTQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36169">
        <property type="entry name" value="ETHANOLAMINE UTILIZATION PROTEIN EUTQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06249">
        <property type="entry name" value="EutQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13551" checksum="DE058D6E9B7A3919" modified="2022-01-19" version="1">MASSTPETFKHYANAQTDFTKTLPLIGNENAFLGDIFSSDRKDNPISSGFYRLDPGTPLVYTYTYDEMKIIVDGDFEISDQSGQSVKATKGDVFFFPKGSVITFKTEKGGLGFYCGQRGKDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Y4</accession>
    <name>A0A8H6C9Y4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003575</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149185.1">
        <property type="nucleotide sequence ID" value="XM_037294497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Y4"/>
    <dbReference type="GeneID" id="59331986"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="280"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="384" mass="43454" checksum="B65829D0D194A92D" modified="2022-01-19" version="1">MYPVSVVLIKCSILSLLYRIFHVPRLKIYAATIGVSVISWGITTLVVIIFNCNPINAYWDTQITNKACVNTLHYFLGIQIPNIILDVVIMILPLPYLWRSHITLSSHKAGLLFVFTLGGFVIIAATLRLNTIAVHKHSVDFTYDLIDLSVWTALETSTGVLCACFAAMQPLLDFAIFGILDTERSTPSTAGRVARPSPEPESTDRQNRWSLKQWPRHNTAPPALENAGLRPIRDMIRRGKHTASSSWGFNQPLPPDFQDEPLRSTQPAETIRHLLRKGYQTERSSFGANQPIPPDPTDEPMPIRDGRNEEEEGEEEGEEEGHDTYDIERATETRTSQKTKPAGVSRFNAFNDVDNIAREERSRRMRKKMESAGWPMTGERRTWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA43</accession>
    <name>A0A8H6CA43_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003933</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148901.1">
        <property type="nucleotide sequence ID" value="XM_037294854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA43"/>
    <dbReference type="GeneID" id="59332343"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF62">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="3">
        <location>
            <begin position="146"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="477" mass="53442" checksum="5DF2EBBC00C1ECD8" modified="2022-01-19" version="1">MEMHRRNQDPFDGGLPSSGNSIYSPDQTPQQTPYRRRSLETGSNTGSDSINPAPRNPFNATPLGTSKNSSAIHIHQQKQNQKYFRSRRVKKGEVDHPWKATKDPREKWVTIIPLIGLALGFLVAGYLVYNGLSTIVNHTYTLVLDEDWSKGFNTDVWTKEVNLGGFGNGEFEQTTGTEENVMIENGKLIIKPTLQDETLLTTNNNLTLPDCTSTQYYDCNAATNVTNGTIVPPVKSGRINTLKGATIKYGRVEVVATMPEGDWLWPAIWMLPVNNTFGAWPASGEIDIAESRGNNYSYATGGNNIVTSTLHWGPDQKDDGYWRTSKKQSALHTTYAAGQHTYGLEWSEKYLFTYIDTRLLQILYNPFNEYLWTRGQFPLSNSNGTTLTNPWVNDPNLNAPFDQEFYLIINLAVGGTNGWFKDGAAGKPWVDKSKTAPKDFLAGKDQWYPTWKPETAQFTISSVKMWQQVGDKPNVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA68</accession>
    <name>A0A8H6CA68_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_004169</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149135.1">
        <property type="nucleotide sequence ID" value="XM_037295088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA68"/>
    <dbReference type="GeneID" id="59332578"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF49">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034534281" description="chitinase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="11">
        <location>
            <begin position="147"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="451" mass="47303" checksum="D8DD7EBE2CC15D1F" modified="2022-01-19" version="1">MFYSSTPILALGAISLLASAGHAEANRREPQIHNSRRQAPAGFVEVPLAELQMLQSEYSTFHGWMTAYLSSANSTDPQTAQLNQDVLAYDMWINIFLSKYANSTNAPAAALTPAAAATPAASSTAAAPYAVVANSSTNGSFSAQSSSNLAVYYGQSPATDQFTLEQMCQDQNVDIVVLAFLTTFFGPAGQPVINFGPATGGTPTLGAQKINATGLLDCPYLAKNITTCQSLGKKVLLSLGGAAGVTNFTSDAQATSFATDLWNLFGGGMTNSDLRPFGSVKVDGFDVDNEDHSTKYYDTFVSSLRNTYAGDKSKQYYISGAPQCPRPDASIPLGSMQTMDFVFVQFYNNAAAGCDIGQPGFVDSFKAWSSDLSGNGTVAGGGPKLYIGAPACEACAGKGYLEPANMTSVIKSAMMAGVKNFGGVMLWDGSEAKNNTGVEGDYLQVVKSALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAD9</accession>
    <name>A0A8H6CAD9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003677</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149287.1">
        <property type="nucleotide sequence ID" value="XM_037294599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAD9"/>
    <dbReference type="GeneID" id="59332088"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF32">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="5">
        <location>
            <begin position="108"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="5">
        <location>
            <begin position="261"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="491"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="495"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="531"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="692" mass="77384" checksum="BA60774110DD07F7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAR8</accession>
    <name>A0A8H6CAR8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149513.1">
        <property type="nucleotide sequence ID" value="XM_037294132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAR8"/>
    <dbReference type="GeneID" id="59331620"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886:SF1">
        <property type="entry name" value="NACHT-NTPASE AND P-LOOP NTPASES N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886">
        <property type="entry name" value="SESA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="138" mass="15391" checksum="EAC446410FFB8732" modified="2022-01-19" version="1">MTPAFGFSVGDFISAIGLVKRVTKALKETGGAPTDYQLVVIELKGLKSILRHLETLKPTEDNVTHINAIRGMAVACQLPLRDFMLKLEKYESTLRPYADKRSLRAMPKLTESDRQTLTPMDSPDQEKHSDLMSMAAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBA0</accession>
    <name>A0A8H6CBA0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_003235</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="14"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149539.1">
        <property type="nucleotide sequence ID" value="XM_037294158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBA0"/>
    <dbReference type="GeneID" id="59331646"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23285">
        <property type="entry name" value="beta-trefoil_MIR_PMT4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000044">
        <property type="entry name" value="Dolichyl-phosphate-mannose-protein mannosyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF51">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="600"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="638"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="732"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="330"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="401"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6220104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="781" mass="88537" checksum="0CC71FDA82CB924A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBJ8</accession>
    <name>A0A8H6CBJ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002978</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149980.1">
        <property type="nucleotide sequence ID" value="XM_037293902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBJ8"/>
    <dbReference type="GeneID" id="59331390"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="95" mass="10477" checksum="F0FB5B490A34C170" modified="2022-01-19" version="1">MDFRGQGVVICEIPGAILQKFCFPISNSYRWVAAESTAEGLEETFIAFPPAVEARFEASSPSLQLQIHPIHTAPEIGDYQGKRQSVSPDSLVQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBK4</accession>
    <name>A0A8H6CBK4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7580 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149948.1">
        <property type="nucleotide sequence ID" value="XM_037293870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBK4"/>
    <dbReference type="GeneID" id="59331358"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056002">
        <property type="entry name" value="DUF7580"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186:SF4">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24476">
        <property type="entry name" value="DUF7580"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7580" evidence="1">
        <location>
            <begin position="171"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="547" mass="62220" checksum="1F1644AAB07C73B6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBM0</accession>
    <name>A0A8H6CBM0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bromodomain associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003423</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149726.1">
        <property type="nucleotide sequence ID" value="XM_037294345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBM0"/>
    <dbReference type="GeneID" id="59331834"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00076">
        <property type="entry name" value="HFD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006565">
        <property type="entry name" value="BTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07524">
        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00576">
        <property type="entry name" value="BTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromodomain associated" evidence="6">
        <location>
            <begin position="4"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="248" mass="26769" checksum="7700EFBAB027C3BE" modified="2022-01-19" version="1">MSTNPLHPSLLRPPILHILRAAGFTSTRPSVLDTLVDLASRYLTILASHTARHALLRHPSQPHQPPDTITITDVRLALQDAGALYPQLSTMEEQVRGEEDMRGIEAFISWCTGPSALEIRRIAGLAPEPSTATVDLSANAALAGKAAAETVLVERPEDFLTQLKKKHAKTRDGEESRWAGTALGREKEREGGVKIEGWEGVEELGDWAERFRRKGDGVVEVGTGVNELGAGRSESSSPLSEISAVTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC23</accession>
    <name>A0A8H6CC23_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150112.1">
        <property type="nucleotide sequence ID" value="XM_037294034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC23"/>
    <dbReference type="GeneID" id="59331522"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="456" mass="51022" checksum="C7F7EC45F69761DA" modified="2022-01-19" version="1">MPHHPPEYNLDQAHATPRSSYRQSTSHRPLSSLRQSAWLSESRLRTSRSLSRMSFTSRRSAPRRPTIGAPSEFRRVQSGRVSPLRRSPAFRPLQLSIYLPGNELPDLPIFWEDGADKVEDVTVERPVQALVKSRSDSMLLRRLSSSFSIPRKPVPSRTSSLDASRFSMDSQFTLNWVGGPTKSRSIDHLRSKSIERRPSFMTTRSAQDFLDALDVRDAHLPQPPPPAIRSNSEPPYTIYRRASEQSLRLRTHLEERQSLEARLPNCATIQEEISPLSPRPGEQMPLSPISDRDDNTDSSPYDEQPLQWQESIQRDARAEVPVMSKARSSSGSSTLLNAPTPLLDPFRPDTAMNERPIVTATISGNTHSSFRDRFSQWILKALPALPTVERSSASLSMYDISGPTELHSPWSFPSPRPHTKQSSMSSSWTLGGGRGASFDVEKTPLPPQVASVGVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC48</accession>
    <name>A0A8H6CC48_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TPR-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002913</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149916.1">
        <property type="nucleotide sequence ID" value="XM_037293838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC48"/>
    <dbReference type="GeneID" id="59331325"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="590"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="624"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="795"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="965" mass="107632" checksum="1E4A9AAEA2061C05" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCF1</accession>
    <name>A0A8H6CCF1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150307.1">
        <property type="nucleotide sequence ID" value="XM_037293478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCF1"/>
    <dbReference type="GeneID" id="59330965"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="194" mass="21112" checksum="7035638AA4FC1176" modified="2022-01-19" version="1">MDAPKPNRDVGPTLKTRSDDPKTEPEETASSRVQVFVLPADGTSPRTEFLPTIDTITNVYSFPPTEFRPVINPSSETSSGPAGRDDTTQANANDHVVTSSQLTLPDPCHGHWPADAWEKRGVFGGGFFHFLVTLLEEGLHPNPWTKGIFFGDVFVLVFFGTTGSPVLKDIFPKAEATPELESALLQLVTASFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD11</accession>
    <name>A0A8H6CD11_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secondary alcohol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150560.1">
        <property type="nucleotide sequence ID" value="XM_037293731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD11"/>
    <dbReference type="GeneID" id="59331218"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038966">
        <property type="entry name" value="TMA17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40422">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40422:SF1">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17640" checksum="1DC4707A4B340D1C" modified="2022-01-19" version="1">MSSTAIPIPPQRFAEAIKELPLANLHSKAAEVRNSIAHLLISNQQLQPFADEGDSDCAEAIQENLVVTQRMEERISLLKREVEGRGFKWGEDETKPENVESNGHAGVEEIERVSRITSTRGGPSTPRSGGQLGDEELARRLREQMEEDDDDEAQDGVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD79</accession>
    <name>A0A8H6CD79_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 16">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_002797</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="function">
        <text evidence="16">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150551.1">
        <property type="nucleotide sequence ID" value="XM_037293722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD79"/>
    <dbReference type="GeneID" id="59331209"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 16" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6221116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="183" mass="20951" checksum="CC6C2F642A2809FA" modified="2022-01-19" version="1">MGLTFSKLFDKLWGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNIQFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDSNDRDRVVEAREELQRMLNEDELRDALLLVFANKQDLPNAMNAAEITDKLGLHSLRQRAWYIQSTCATSGDGLYEGLEWLSNSLRKAGHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDE8</accession>
    <name>A0A8H6CDE8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150634.1">
        <property type="nucleotide sequence ID" value="XM_037292981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDE8"/>
    <dbReference type="GeneID" id="59330468"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="143" mass="15771" checksum="F21E1991CD857481" modified="2022-01-19" version="1">MDMMMMLQEESGNILFGYGTFQGFQPSKVLVLGRKSEVPSSRRQAVNNHWVGTKVIASSPSAPTFTTPEKVAVKRFAQWQSRLPSTVEYCLLILQDQRGVGSAAGRKSNPGGGTGTDDELRLGITTETRRNNRMLTSHFHSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDF0</accession>
    <name>A0A8H6CDF0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Dicer-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HO133_002205</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the helicase family. Dicer subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150785.1">
        <property type="nucleotide sequence ID" value="XM_037293132.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330619"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050688">
        <property type="term" value="P:regulation of defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18034">
        <property type="entry name" value="DEXHc_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000032">
        <property type="entry name" value="Dicer-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.380">
        <property type="entry name" value="Dicer dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038248">
        <property type="entry name" value="Dicer_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005034">
        <property type="entry name" value="Dicer_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF37">
        <property type="entry name" value="ENDORIBONUCLEASE DICER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03368">
        <property type="entry name" value="Dicer_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51327">
        <property type="entry name" value="DICER_DSRBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-0930">Antiviral protein</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 15" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="45"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="387"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Dicer dsRNA-binding fold" evidence="21">
        <location>
            <begin position="578"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="18">
        <location>
            <begin position="943"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="18">
        <location>
            <begin position="1114"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="17">
        <location>
            <begin position="1328"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1411"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1441"/>
            <end position="1452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF6221350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1452" mass="161458" checksum="5C1B17B382A9B1AB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CED3</accession>
    <name>A0A8H6CED3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151357.1">
        <property type="nucleotide sequence ID" value="XM_037292818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CED3"/>
    <dbReference type="GeneID" id="59330304"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="79" mass="8793" checksum="78FD3304BB88E2C9" modified="2022-01-19" version="1">MTSPSPESTAVTEGITTPHTNIDDIEDTNLEQKIAHIRAKYPLRSTRECEEALQLVNNDVDKTLAFLAYLVPRIPGIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEL0</accession>
    <name>A0A8H6CEL0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151553.1">
        <property type="nucleotide sequence ID" value="XM_037292134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEL0"/>
    <dbReference type="GeneID" id="59329620"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678">
        <property type="entry name" value="AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678:SF34">
        <property type="entry name" value="OS04G0183300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034079642" description="Amidase domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="68"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="604" mass="64943" checksum="C6C9E8D084A8315D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFR1</accession>
    <name>A0A8H6CFR1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001455</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151804.1">
        <property type="nucleotide sequence ID" value="XM_037292385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFR1"/>
    <dbReference type="GeneID" id="59329871"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1590.10">
        <property type="entry name" value="glutathione-dependent formaldehyde- activating enzyme (gfa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337:SF40">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337">
        <property type="entry name" value="GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="6">
        <location>
            <begin position="46"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="212" mass="24414" checksum="ACCF6740A660FEE4" modified="2022-01-19" version="1">MAEHASSYRANGDNGDEGNTEGDLNAWKHRAPYKVHDSDPNFHARYEASCHCGKIRYQLSREKPLDSKYCHCTTCQKLHGAPFQWAAIFHKEDINFTHGHSDLGWYESSEKTTVHKLPCKVSCAYCRTPIMDEGRNMILLFPTLINFKSPEDRKNFDPSCHMFYTRRILDINDGKPKWSGISNDSELIEDSPEQDKKRKREEDAAEGNDKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFV6</accession>
    <name>A0A8H6CFV6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enhancer of mRNA-decapping protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001515</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EDC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151864.1">
        <property type="nucleotide sequence ID" value="XM_037292445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFV6"/>
    <dbReference type="GeneID" id="59329931"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612:SF0">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DFDF" evidence="7">
        <location>
            <begin position="310"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF N-terminal" evidence="6">
        <location>
            <begin position="508"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="193"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="383"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="389"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="458"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="767" mass="83007" checksum="47E809FC551A2063" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHK9</accession>
    <name>A0A8H6CHK9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_000477</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152851.1">
        <property type="nucleotide sequence ID" value="XM_037291416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHK9"/>
    <dbReference type="GeneID" id="59328896"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="13">
        <location>
            <begin position="91"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="232"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="500"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="500"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="546"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6223634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="651" mass="71981" checksum="F03401712A8CCAB4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHZ3</accession>
    <name>A0A8H6CHZ3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152763.1">
        <property type="nucleotide sequence ID" value="XM_037291328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHZ3"/>
    <dbReference type="GeneID" id="59328808"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="171" mass="19243" checksum="A7BBBB7981CEFEE3" modified="2022-01-19" version="1">MAKTGFIEVDEGRLFWKYDPPVDHEDSADSGPPGCLRPYSVSKPDSQTENPCIFCRWDEQVIYYTARGWYTLRFDLLGYGQSLPSESYVQRGCTPAVKHHDHALAVIKEYSLCRGRAAIDDKFVVIGLSRGASTDLNKTILDSPDMRQITLSTSLCNLRISLPGLFYARVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI23</accession>
    <name>A0A8H6CI23_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sucrose transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000442</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152816.1">
        <property type="nucleotide sequence ID" value="XM_037291381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI23"/>
    <dbReference type="GeneID" id="59328861"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF35">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 45 MEMBER 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="502"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="589"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="616"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="644" mass="70434" checksum="1F19C95B5A7DB8E7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIA3</accession>
    <name>A0A8H6CIA3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152936.1">
        <property type="nucleotide sequence ID" value="XM_037291501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIA3"/>
    <dbReference type="GeneID" id="59328981"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07379">
        <property type="entry name" value="MPP_239FB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051693">
        <property type="entry name" value="UPF0046_metallophosphoest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905:SF18">
        <property type="entry name" value="ESTER HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G03130)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="67"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="324" mass="36355" checksum="C25E0079C2ACD210" modified="2022-01-19" version="1">MSLRNGSDPREPESAPRIVKLPRHRLRSVASTLKSLHRLPCPPPLPTHLLTVVCISDTHCTHPPIPPGNLLLHAGDLSKWGTFSEIQAQLTWLSEQPHKYKVVIAGNHDLLLDPEFKEQHPERWREAAQAASDDKHIDYIDRPAAHLDWGNVIYLQDSSVTLLFHGDRSLRIYGSPLTPRYGLSAFQHPPSKDVWTDKVPPHTDIILTHGPPRGHLDGFKKSGCAFLTQEVVRVQPCLVVCGHIHVGYGTEERVYDQVGIAYEQISGQGAGWGTLLGMAWGVTWGYLIPRSWRRSERKTTFVNAAVVEGWENHRVKNEAVVLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIG0</accession>
    <name>A0A8H6CIG0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">KOW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010534</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153020.1">
        <property type="nucleotide sequence ID" value="XM_037301386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIG0"/>
    <dbReference type="GeneID" id="59338924"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22682">
        <property type="entry name" value="Ribosomal_uL24m-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="430"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="460"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="53715" checksum="B8A4F3A9BEC2BB78" modified="2022-01-19" version="1">MRPFRRPPAHLPHCSRCLHRSISTTATATRKRRKDRDAQKTIDHDLQQRASILRTRIREARVARREDWILGPLAPRRETGGMEYGTVSSRELKGVKGGLARGLLTQRGLGGITAAGKRGSKGKGVGMSWKESLIYEGDRVAILSDTGPERRDKWKIGTVTSVREKEREVLVKGLNKVDLNVLADNKPAIESKLLPIPLSSVRLVHNLPDPYTRIHTDKIISRLIPLPDEKKAEIREEFKAGEIDLEEMKTRMGGRAIPGSKEVYGDWVVIPLPKARKNKKVETKEHDSDTVRFEVEEKTWTPTLLRTPMPGGVIDELRNKYSRFRTRHDAGYQLALDNRARRKAEYKAWAESGGGMLMTPAKEARQMEREKLKEKGEPTLEKELLERIGEVMAKHGVEMTGKRRRETEKNLRGERVVKWGSDVEIPGEKKAERAVLEEEEDEDDEEWEEEEDEVGDVVIEEDKTSEKRPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIW8</accession>
    <name>A0A8H6CIW8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Galactose oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153041.1">
        <property type="nucleotide sequence ID" value="XM_037301407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIW8"/>
    <dbReference type="GeneID" id="59338945"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="499"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="657"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="772"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="850"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="899"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="669"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="710"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="808"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="915"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="935" mass="100534" checksum="C4F1CAA8EA359A19" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ02</accession>
    <name>A0A8H6CJ02_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153423.1">
        <property type="nucleotide sequence ID" value="XM_037301789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ02"/>
    <dbReference type="GeneID" id="59339330"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="214"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="262" mass="29409" checksum="A67266BE47DB9A9D" modified="2022-01-19" version="1">MVDEKVGPSASGKMMVMRMTDKERNDGHAKAGKAGTAKDADEKSEIPQPRRAVHRRLPGSGTHMSSLSTRSDVSQRITKFVRYTAPRHHNLARGTSTRVKQYSLGAFKKPPSLHLPTLLKDFQTTQETLQQLKHSNTMPSPPDFNEKASTQPDQAQEESTPTTADQASGPQNANIFDLQQTIVSTAPPNLPPDMIPLCYIHPRIHTRLRPPTPQRRLEAREAQLEAQNAALKKEVEGLRTKERELQMTMRETQMHLDHSRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK59</accession>
    <name>A0A8H6CK59_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009919</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153593.1">
        <property type="nucleotide sequence ID" value="XM_037300776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK59"/>
    <dbReference type="GeneID" id="59338312"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="28813" checksum="A0C48758948504D7" modified="2022-01-19" version="1">MRLFPRSILGSLGSVTSPKKGKDKGAGQDAAAPSVPGAGKDIPTPSIPGVGNDLPTPSVPGIANDIPTPNVTDLHKDSPTSSAAGAAKGTPVPSAAGAESASDRNVNPSIFTTFVTVSVTDISTVTSADVTVTIVQHDLATVATTAPTTVTVIQHTLPTTVATTIVKTKSVTPSTEHIAAAHSTAPLPPAVAAMPTAKKHVDLAVVLSMIFGLITLGLLGAVYLLVTRSRRRAAKAKQTTGMKTYAEEFEWPRQRPVEMDGIKRNDEWGPVQKHARGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKJ1</accession>
    <name>A0A8H6CKJ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010100</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153773.1">
        <property type="nucleotide sequence ID" value="XM_037300956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKJ1"/>
    <dbReference type="GeneID" id="59338492"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015343">
        <property type="term" value="F:siderophore-iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000197">
        <property type="entry name" value="Siderophore iron transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF87">
        <property type="entry name" value="SIDEROPHORE IRON TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="580"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="593"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="610" mass="65751" checksum="4E27FCEF57F47345" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM60</accession>
    <name>A0A8H6CM60_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009793</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154500.1">
        <property type="nucleotide sequence ID" value="XM_037300652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM60"/>
    <dbReference type="GeneID" id="59338188"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000061">
        <property type="entry name" value="MFS sugar transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF26">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="420"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="27"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6225791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="555" mass="61463" checksum="4A417AF4D08DF443" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMS4</accession>
    <name>A0A8H6CMS4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">nicotinamidase</fullName>
            <ecNumber evidence="6">3.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Nicotinamide deamidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009199</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; nicotinate biosynthesis; nicotinate from nicotinamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.19" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154886.1">
        <property type="nucleotide sequence ID" value="XM_037300060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMS4"/>
    <dbReference type="GeneID" id="59337594"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008936">
        <property type="term" value="F:nicotinamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019363">
        <property type="term" value="P:pyridine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052347">
        <property type="entry name" value="Isochorismatase_Nicotinamidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11080:SF2">
        <property type="entry name" value="LD05707P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11080">
        <property type="entry name" value="PYRAZINAMIDASE/NICOTINAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="9">
        <location>
            <begin position="126"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6226333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="277" mass="29983" checksum="1F9069387473F4B3" modified="2022-01-19" version="1">MNDAPTSREYFKPALLVVDMQEDFCGPSGSLAVEGGRDIAPTINSLLSLPFALKIATKDAHPQGHVSFAISHPPPNNKAFESAVKISNPMRRRRTRSGRVGGSNIIKTTTSNDKSHYSSNCIPRYLSDRTQFHEIPLWPVHCVQGTKGAEIIPEIDVSKLDEVVEKGKDSGLEMFSGFADIFGSKSPEAASQDLAGLLHCAGITTVFAVGLAGDYCVKCTALDAKKEGFEVYVVKEATRSVDAGENGWGKATKEMREARIEIISVEGPEMARVKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMW0</accession>
    <name>A0A8H6CMW0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155007.1">
        <property type="nucleotide sequence ID" value="XM_037300181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMW0"/>
    <dbReference type="GeneID" id="59337715"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658:SF8">
        <property type="entry name" value="17-BETA-HYDROXYSTEROID DEHYDROGENASE 14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="163"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="58623" checksum="979DF796A2B20998" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNX7</accession>
    <name>A0A8H6CNX7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acyltransferase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155164.1">
        <property type="nucleotide sequence ID" value="XM_037299163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNX7"/>
    <dbReference type="GeneID" id="59336693"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050879">
        <property type="entry name" value="Acyltransferase_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23028">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23028:SF134">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08520)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Acyltransferase 3" evidence="2">
        <location>
            <begin position="79"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="508" mass="57973" checksum="BF8E8758E9978EAA" modified="2022-01-19" version="1">MDFFKRTLTSSLHPSATDEDLETLLKAEDGGTSFSTKYGLPTTASRFIKKIVLAILPNPIQRRLAPHFYKPSRIHSTSYLDGLRGIASFIVFVHHFTNWVNPYLAYYGVDNEKRPSSLVQLPFVRVIYSGRPMVHVFFVISGFVLSRKPLRLARAHKFDELHKTLSSSVFRRSIRLYLPAAFSTFFVLLLIRAGWARQPVDGGFLAQVGDWTSAMFDMTKPWQWDVIQNLRYDMHTWTLPVEMSMSMLLFVTITGLSRCKVPIRLGMMIMIMLYCFQSGRWAAVEFLGGAWIAEVDLIQEERATNAGNLQSTAPVEHLDGDQLNPTELPKAATWSGLCWTLLWWLQLAIALWICGWPNHDVEKAPGLAWLVKHAPEPYFSLGEEHYTDWRATPWYILASLQIVFACQQLPPLQRFLTTGPIQYLASISFALYLMHGPLFDSIGGKIMTPILHSVTSMDEAGVWELFFVWIAGLFALGIPCLWAADLFWRFIDRPCVDFARWVESKCTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPL0</accession>
    <name>A0A8H6CPL0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008347</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155215.1">
        <property type="nucleotide sequence ID" value="XM_037299214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPL0"/>
    <dbReference type="GeneID" id="59336744"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451">
        <property type="entry name" value="MARVEL DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451:SF1">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
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        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
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        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="37636" checksum="3657035BB76BE9EF" modified="2022-01-19" version="1">MQIYTSDKTSPRSFPWLWYLRLVALVVTLIVLGITASNTATFHDIGCDAPGRVSYNLAVSILSLIALVYFILASGPSRTTRLLPWFIWGQLALDALMFIFWLSAAATSSYSCTDLCNACGILDGFVFFDSESCECSDFFYKRDYSPRRGNVLQPRRSHSSSPSSGSTVGGTIAAKQAFDAIMTVLFALFIAADIFWVLKSRRSGTATAPAPTIGAPMKNEEAGMSAGGGGAPTYTQEPGSQYNYPMAPQGGYTGQPMQNQAGSPQQSMPQGAYSGGHYPQGQPQYSSQTSYPQGQQVPLQTYPSPQGEYPPQYGGSAPQQASYPEPRDGVSEMHSPTEPPKMNDPYHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPP0</accession>
    <name>A0A8H6CPP0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155359.1">
        <property type="nucleotide sequence ID" value="XM_037299358.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59336888"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="bZIP_Zip1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bZIP"/>
    </dbReference>
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        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="238"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
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            <begin position="136"/>
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        <location>
            <begin position="231"/>
            <end position="279"/>
        </location>
    </feature>
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        <location>
            <begin position="300"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
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        <location>
            <begin position="47"/>
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        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
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            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
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        <location>
            <begin position="202"/>
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        </location>
    </feature>
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            <begin position="377"/>
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            <begin position="390"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="402" mass="43428" checksum="12463852D08C1FBD" modified="2022-01-19" version="1">MPRASTPSLPPTSMTNRSPVSMTLPSITPPLVSVHPLPLGRAMTPHLSPSSYGPGSVTINNPSGTMDVKQSPFVLPRDHNGAGGRGSLPDLATATSLPGDPYGAPLPPARSPPGRRGSQDSTRYSHMQNLLERKGGMATGPQSVASQGDSPSTQYSSYSQVSHTPPPAPPPSTGQPQSFFTAPFTAPGPASSMAQMGFDAASGSTAGGSTYQMMTLDTEQGPIQVPVDVQAASKVADEKRKRNATASHRFRQRRKEKERENSQNIAKLEDQIRQMEEEREHYRRERDYFREVAVRQPGQTHVLARPLSPRQRRHSSIGGSMGYPNVQFEGPETGNRTGGRNTRRRTSAYVPPTGPSPQSAEPARPMPQYEQRMASKPTEHIQGGDHARLSLQTGPFDPSTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPP1</accession>
    <name>A0A8H6CPP1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037299273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPP1"/>
    <dbReference type="GeneID" id="59336803"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="22643" checksum="78E2A9206C382914" modified="2022-01-19" version="1">MLDMHDVHGLRQRKRPNSREWKAFSRQWPLLSYASNHWIRAHALNASLEPTSNYERGGNFGFWVQCLFSLDPQAGIRVARDTQPLYYAGLRAMVGSLISANRDTDLDTLGGRNASTALQVACYRGHYEVAKDLLDAGANPYHRDRYGRSSLFYALCHGNKDIAELLRQYLRSRTDPKGEAASIHVEEAFAAARMFMAGAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPX3</accession>
    <name>A0A8H6CPX3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155755.1">
        <property type="nucleotide sequence ID" value="XM_037299754.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337286"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
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        </location>
    </feature>
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        <location>
            <begin position="741"/>
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        </location>
    </feature>
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        <location>
            <begin position="818"/>
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        </location>
    </feature>
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        <location>
            <begin position="877"/>
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        </location>
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            <begin position="977"/>
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            <begin position="1296"/>
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        </location>
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        <location>
            <begin position="1333"/>
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        <location>
            <begin position="1373"/>
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        </location>
    </feature>
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        <location>
            <begin position="1490"/>
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        </location>
    </feature>
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        <location>
            <begin position="1640"/>
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        </location>
    </feature>
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        <location>
            <begin position="1713"/>
            <end position="1793"/>
        </location>
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        <location>
            <begin position="512"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="596"/>
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        </location>
    </feature>
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        <location>
            <begin position="742"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="780"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="831"/>
            <end position="844"/>
        </location>
    </feature>
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        <location>
            <begin position="847"/>
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        </location>
    </feature>
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        <location>
            <begin position="997"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1032"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1069"/>
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        </location>
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        <location>
            <begin position="1100"/>
            <end position="1116"/>
        </location>
    </feature>
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        <location>
            <begin position="1143"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1223"/>
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        </location>
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        <location>
            <begin position="1248"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1344"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1402"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1518"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1640"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1751"/>
            <end position="1760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1762"/>
            <end position="1781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1916" mass="210006" checksum="0CD13F185B39CAAB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRE0</accession>
    <name>A0A8H6CRE0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156122.1">
        <property type="nucleotide sequence ID" value="XM_037298786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRE0"/>
    <dbReference type="GeneID" id="59336315"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034293031" evidence="1">
        <location>
            <begin position="21"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="246" mass="26784" checksum="F72A4FE67D242AD3" modified="2022-01-19" version="1">MLSTLPLIALFLVQYTTVTIEAPAQTLGGSSSVQSASDLEGQIGDLVIPQEEAKNITTAETRKIRSTLDYATLLSEIGDFTLPSNITAGHVAKVAKQAVPSLSDAEVINIVQALRPEQFSLRSRTQYALVQVIAAVYEGRISHGGWGLDDQDRETSRFLFNELSSPEIGVIDMAGFHRLEISNLRLRARRVTCTLKRRMAIMLSGPTKHFNASSGDACAKPGRTQQAAESLNDGLIELEFIDNRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRP0</accession>
    <name>A0A8H6CRP0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155950.1">
        <property type="nucleotide sequence ID" value="XM_037298614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRP0"/>
    <dbReference type="GeneID" id="59336141"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="225" mass="25599" checksum="36CA8F00FB0E565F" modified="2022-01-19" version="1">MQAFLQTLQAYDNGILREYYPWEYDAEAQQCLERGNKPSYFVTEEDCKFFQQQAKILQDRAAYIRQEWARQEAALQAIQPVTQPVTQPVTHPVTQVATQLATQSDTLQEEELQQANAENTAENTAEKASDNKATTIKNTAQLSKIPRTGLRSTIPRQIATQQAENDTAIWPLISATLLWHTITELSIQEKDLLRRSQPNHYGYGNWTSAGKGHIGRYIFGRIETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7N0</accession>
    <name>A0A8H6F7N0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147163.1">
        <property type="nucleotide sequence ID" value="XM_037297453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7N0"/>
    <dbReference type="GeneID" id="59334956"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="40662" checksum="68EC96840ED1043A" modified="2022-01-19" version="1">MKDFGQAHISTNLDISYPSHPYVESKRSEKIFNIRARGRSSHTLMASSEVDQKFLGRFAKATDRSYPYLSSKLYKVLGLSVILSKKLVRARRLRRLDTTRDTKSLQLYHHIIFLSREGLYIIEEYVLPMVENTRTHALKVLCHKLRASFYHIYVLFHNEPSITQTAIPSYTPSAAEKGKAPVYRNSMRPSPPPLEGGPVQNIGSLPPGLTPVSIPKPSASFLLPALNYIPRASECFEYACKLANTNLSGSHPVRLSVKTEYAAYLYDCLHDGEGSRRLAQVAIKDVYNAQEGMDDDMFEDAAELVGILGKMMKRGLGAGSQGSGSTPRAGNESTPSSTPRPGKVDKFEKELPDLPSPQQEEIAGVCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7Z2</accession>
    <name>A0A8H6F7Z2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147990.1">
        <property type="nucleotide sequence ID" value="XM_037296809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7Z2"/>
    <dbReference type="GeneID" id="59334310"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39609">
        <property type="entry name" value="RFEG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39609:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR RFEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="127" mass="14434" checksum="AE107945398FB2F2" modified="2022-01-19" version="1">MPSTNSTSSNAYTTYFVPGYSISRHIMFTHIQYHLGPYVSVRPFTYQQREGYLISNPGSALTKVSILPSGSAVSMLTCTQSQIEDLQYMSRQYEQQEAERMIRASGALDRADVYINRPIPVQQRYGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8G1</accession>
    <name>A0A8H6F8G1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein UsgS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148048.1">
        <property type="nucleotide sequence ID" value="XM_037296867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8G1"/>
    <dbReference type="GeneID" id="59334369"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38421:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38421">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN USGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="40196" checksum="31C3496196F6D099" modified="2022-01-19" version="1">MSTTDRQQLLSVRVSPSEFLLQFLWVVYAPRIESGANWRLLLQIITVKVSLWALGFFINFDSVTWDDKLVGGMDFLANSVLQVPFFLMSLMRYITPTLDRMFMDSLQWVDQTYYAKHKSEDPKTLRATYFAELRMYSAHGDTSKHRAPMDAALAFLIRFGRRAGISLAIYALSYLPIVGRFVLPAASFYTFNNAVGPIPATIIFGSGIFLPRRYLIVFLQSYFSSRSLMRELLEPYFSRIRFTKEQKKHWFHDREGVLFGFGVGFYIFLKIPLLGVLIYGIAEASTAYLITKITDPPPSPAYSDKFAESQVKWKNKQKFLELPLHNLDANNADTVGKEPIEAFDSPGKKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8K8</accession>
    <name>A0A8H6F8K8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005025</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148635.1">
        <property type="nucleotide sequence ID" value="XM_037295937.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333431"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000182">
        <property type="entry name" value="MAP kinase kinase kinase mkh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000387">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF48">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BCK1_SLK1_SSP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1814"/>
            <end position="2084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="256"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="329"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="386"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="564"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="721"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="833"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="954"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1129"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1229"/>
            <end position="1615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1658"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1699"/>
            <end position="1770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="564"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="593"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
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            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="868"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="954"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="990"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1154"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1191"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1308"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1380"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1401"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1438"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1465"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1572"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1596"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1713"/>
            <end position="1729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1757"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="1843"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2115" mass="230326" checksum="2748A5B5D31AEEAF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8Y5</accession>
    <name>A0A8H6F8Y5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
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            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037296457.1"/>
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    <dbReference type="GeneID" id="59333954"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="154" mass="17010" checksum="13B1D58E5415D6CA" modified="2022-01-19" version="1">MSTSKVFLSGSSEALNVHPVLSPASKKDLEDALDETMSAEDAQKKAWVEDNRRDLFNAFATGRKVDLSSHKNIEVFSAVEGTDEDLPMRKSMRGTQTVQKVMLYVEGTEWTRSSRVLDNLAAMIQAEDAGFPDDEDWAVVEKPVGGLGFKHCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9F0</accession>
    <name>A0A8H6F9F0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003308</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037294231.1"/>
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF251">
        <property type="entry name" value="EG:BACR7C10.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="52"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="407" mass="45044" checksum="76C0AD169DBB1D5C" modified="2022-01-19" version="1">MATLPKHIYTQITPDAGTGLFASEAIPPGVEILRIERPLVSVLDSPHLKDTCSECSLWLPQNDDDRDPKCKRLKACQACKVTKYCCKECQTQSWKKHHKYECKIFAKLYPNVLPNTVRIVMQLLLRLNAKSLPDPEWQAFLDLQSHVDEIRSSQIKNEDGLTTWHAIELMSQAASSYSGSQEPISFIQTLTARILINSHTLTTPNLDPLGITNGLPDPRPDHNFDAVQARAVGLQAEASTLPQDRAAALLKSALGFLGHYPPHRQPSPSILHTAFLNAIATQSWAIALSYALKAYFLIDPLHYRSSWHPVRVVRKWVLLRLVTQIAGIVSEGDGSIKGLEKFGINWQIVAVGLFHEISDGAPHSHGSNSPFTAEVKAFGEGAGMGRGKCEKALLEEEWAKLRKVADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA23</accession>
    <name>A0A8H6FA23_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mucin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149639.1">
        <property type="nucleotide sequence ID" value="XM_037294258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA23"/>
    <dbReference type="GeneID" id="59331746"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="56419" checksum="3ED8E54B0DA3B305" modified="2022-01-19" version="1">MTCTCTSLNSSSNRINNNNEWANLPPAVKRKYFSSLERLRLAQNSASATKAASVKSCHGALKASKPKSSPSRPALARRNSSYFKNPPILRRKTPSPSSDTFIPSDAQWFLRLPNKVQRKHFTREERLALSGHRDSIVLDASDEKFCRFYRQGREGNRSVPTLRTSSYSSLSSLSTFDDEQPADSAIDMDDSMIWFDEDNDVDLSAKLQLDDYHEFMTKAAESDIKPSKRRPSFRRALSLNQLPFGANTSSVPPLPKTTYATPFATTPLPQPVHQHAESRPFLALAHGPQTSNSAIEASTKYYQDPKARLKLRVYLASPQNFDEAIEFGFPSMEDTEYRPLSRPSLSRAHKTAPCKTFLHDDIPSLFDALDDDDDAESTTSLPERDAPYTPMDSVFNHDAALLSPSVATSSGSSNTLFPKSTVRHHTSEPFAQLLAGSREMTLRMTLTRPDLRADEKALYANTTGDDPLALEHLPPTKKEADIWDQLPKERGTLKKFWKRVSGKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAQ8</accession>
    <name>A0A8H6FAQ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151007.1">
        <property type="nucleotide sequence ID" value="XM_037293354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAQ8"/>
    <dbReference type="GeneID" id="59330841"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="181" mass="19895" checksum="2300555FA2657A02" modified="2022-01-19" version="1">MTEVWHSRSAQGCKTFADPVEDSVPAYVKRCNAKANGSDKIDTFIQPPSEKASTYTYAYCILRESLIDREATTEDFNAFLSIAAPQALPEEGLLHLRAIFLVDASGSTLQPDQKIQFEGEVLQHASKLMLMALIALHLAKKLVGVKVLEKANLEAFGAAAGKRIYGGDRMPEGIPEWLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB29</accession>
    <name>A0A8H6FB29_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001808</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151275.1">
        <property type="nucleotide sequence ID" value="XM_037292736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB29"/>
    <dbReference type="GeneID" id="59330222"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="489"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="61428" checksum="6723FB0D017483F9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB88</accession>
    <name>A0A8H6FB88_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UNC93-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001973</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6222005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151440.1">
        <property type="nucleotide sequence ID" value="XM_037292901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB88"/>
    <dbReference type="GeneID" id="59330387"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06178">
        <property type="entry name" value="MFS_unc93-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF59">
        <property type="entry name" value="UNC93-LIKE PROTEIN C922.05C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="57027" checksum="019F7ABE76A502A4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBA3</accession>
    <name>A0A8H6FBA3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1760-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151500.1">
        <property type="nucleotide sequence ID" value="XM_037292081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBA3"/>
    <dbReference type="GeneID" id="59329567"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013877">
        <property type="entry name" value="YAP-bd/ALF4/Glomulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040347">
        <property type="entry name" value="YBP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020:SF1">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08568">
        <property type="entry name" value="Kinetochor_Ybp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="684" mass="74750" checksum="D1B7A30BE94AFA49" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBF9</accession>
    <name>A0A8H6FBF9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151712.1">
        <property type="nucleotide sequence ID" value="XM_037292293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBF9"/>
    <dbReference type="GeneID" id="59329779"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="130" mass="13897" checksum="2D27316B9F49C5E8" modified="2022-01-19" version="1">MPENLEIKVMIGGGARAHSPRFEKLNVVVHAWIQGLKMPTAQHKISCSARPAAAGLWSVLLPEVLASTNTKKNRNVIASIPNVEMAAPIKPPHGIQRILQVDNLQAKGDTGPQIDSAANVGNRNAAREEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBS6</accession>
    <name>A0A8H6FBS6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151968.1">
        <property type="nucleotide sequence ID" value="XM_037291606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBS6"/>
    <dbReference type="GeneID" id="59329087"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="258" mass="29782" checksum="EDBB9E9E11BF959D" modified="2022-01-19" version="1">MEECKRCVKNSISPLAPIAEHPPAIPCNWNLAFQKHAANIHLRLPGTNNYDFLKDCEKPLQQIVQHFTNLRTLRLDFLDVSTGQQSAWDVDEYLMHDLLSTLRSTIWSTMKEFQAPDRSAGALKEIVLTGLPQNDLRYVMKQYARLLARNGRFCVGWGVKRKRYELLGARGDGEVTKREDLELLWMRVEEEGEWIAGECHKKGSRWLFTRQKPESKASWSQSPPLPEPEQSQESKAWDQEFGEDLETLASLDNLRGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBV5</accession>
    <name>A0A8H6FBV5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA topoisomerase</fullName>
            <ecNumber evidence="3 7">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_000759</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3 7"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152058.1">
        <property type="nucleotide sequence ID" value="XM_037291696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBV5"/>
    <dbReference type="GeneID" id="59329177"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000005">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF21">
        <property type="entry name" value="DNA TOPOISOMERASE 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="9">
        <location>
            <begin position="4"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="10">
        <location>
            <begin position="169"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="529"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="544"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="656" mass="73775" checksum="FA45BA798BFB44CA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCB5</accession>
    <name>A0A8H6FCB5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">HO133_000620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="GeneID" id="59329038"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="31561" checksum="4B6D20BB31D60B0E" modified="2022-01-19" version="1">MAPPASQTKIIGKPSGELVPSTSYQAFNTPDSQLMILDLSQDTINGLMTAFGGMMRQEMQRFEQRLERIEQFLGPALYQNPTSSPNPTPPPNATPLPSIEIPQQKLSHTNDSSYHTNPGHLRAEEVGYFDPEYQQEQEQEHRPLQQANVEKTAEKFTENTTEKSSDNKATIIKNTAQLSKIPRHILSTIPRQIATQQAENDTTIWPAISACLGNITLVWHITAMSKQEKDLLRRSQPNYYDQQAKATHISLTSHRMAMRSMRKMRRRIRSIMATSNGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCE6</accession>
    <name>A0A8H6FCE6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000801</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152099.1">
        <property type="nucleotide sequence ID" value="XM_037291737.1"/>
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    <dbReference type="Proteomes" id="UP000593566">
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    <dbReference type="InterPro" id="IPR021840">
        <property type="entry name" value="DUF3433"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37544:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="529"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="573"/>
            <end position="592"/>
        </location>
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        <location>
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            <end position="665"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="685"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="806" mass="91314" checksum="B55055E0AD775F99" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCZ7</accession>
    <name>A0A8H6FCZ7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152556.1">
        <property type="nucleotide sequence ID" value="XM_037291121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCZ7"/>
    <dbReference type="GeneID" id="59328600"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="261" mass="29307" checksum="F9D78543043BAFDB" modified="2022-01-19" version="1">MDRYGASTRDFESDDDYEYTRRGAPLDRDDDYDRGYGRADLMHERETFHGERSGLGHRSEFSDDEDTDHGRHGRPTHRPDASSSDRYRSSADVVRGDGPHRRGYPTGTPDDSDSDYSEVERGDRDIRSRGLGRRGETRRPDPRFRGESSEDESDLDSRATREGHRGPGIRRAQPLRPDVRFNGGGSAGRGAARASTTDDESDSEQYGSRRGNIGDARGGMHPGCRGHLSEDDDLDDSEDEGMRRGRRGAISFGRPSTREFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD83</accession>
    <name>A0A8H6FD83_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IBR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010787</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153272.1">
        <property type="nucleotide sequence ID" value="XM_037301638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD83"/>
    <dbReference type="GeneID" id="59339177"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="IBR" evidence="5">
        <location>
            <begin position="103"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="241" mass="26624" checksum="CEF38DC06598F5AC" modified="2022-01-19" version="1">MADMDDAMMILIPATPVFGEASAQGIRETVRREVDFLRTMECVVCRNQEVDSNVMTAPCGELYCGGCVNEVFDRAAKHEFNFPPKCCGQIITLANAESFLSSDIYDKFQKKSEEYSTTNRTYCSDPECVTFISPKAVDDGQAKCPACQKLTCIVCKEEVHEGGCLEDPAAQSLMTAAAEAGFQQCQECKRMIERINGCDHITDEGDPLDEAEGFLQYAVDHVLHIHEDNRIVWRIPAAAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDB4</accession>
    <name>A0A8H6FDB4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152885.1">
        <property type="nucleotide sequence ID" value="XM_037291450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDB4"/>
    <dbReference type="GeneID" id="59328930"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="201" mass="22756" checksum="ED3E37B1D421A82F" modified="2022-01-19" version="1">MVKEMLSLQNTSGTESFRSNYLELRHKAPPAVVLRTDQDFEYSREAVQAKCSTTFDSVDLSSFQRSIYENADEAHWYMNHGPGGLPDFNWTEERDKFELEHQQTQRPLEHTDHSPKTPSVSPLTSLHPPKAETTPVIVPNDSPLTPSESSLTDSQPPNSSVEIPANTPQIVIPDDSWPHGLELRRSEKQLEALLRKPQIMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDH2</accession>
    <name>A0A8H6FDH2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_011059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153542.1">
        <property type="nucleotide sequence ID" value="XM_037301908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDH2"/>
    <dbReference type="GeneID" id="59339449"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="188" mass="20610" checksum="26B40DC8A3849D2F" modified="2022-01-19" version="1">MASKVIIVIGELPEGISLVVCEYLLQKPQSARLAVVGRGKQQLEDIKSPIASARRRPFRFLARKTISRPSIVKTGQIGCYVLNPGVWDRGAAISQHLERCSKAAIDHLATEVSIEEPEATTVSVKPGVVNTEMQKQVAIQTCRRTGPQRCGAIHEPSRERCFAPAISARKCFGEIGLRLVRKDRVGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDH5</accession>
    <name>A0A8H6FDH5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_011074</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153557.1">
        <property type="nucleotide sequence ID" value="XM_037301923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDH5"/>
    <dbReference type="GeneID" id="59339464"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000069">
        <property type="entry name" value="Seryl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="244"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="285"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="344"/>
            <end position="346"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="360"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="435"/>
            <end position="438"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6224497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="529" mass="59217" checksum="92B00F53AB46F592" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDU9</accession>
    <name>A0A8H6FDU9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GLEYA adhesin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153268.1">
        <property type="nucleotide sequence ID" value="XM_037301634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDU9"/>
    <dbReference type="GeneID" id="59339173"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GLEYA adhesin" evidence="1">
        <location>
            <begin position="64"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="191" mass="21087" checksum="F3B067F180471915" modified="2022-01-19" version="1">MQYFGFEDPADAYRGTQPDASRFNNNASDINIQGAFPDEINFNTNSTGYYQFPGQINQTDVANYAVVLEGYFYGPQGEYSVSLSDTGTDDYSFLWTGSNAYSAWTNANAAITESIAGQYTQNHTFNLTAESEFLPMTILWINVFSSGSLEFVIYPPAGEGIFVYPPTGGEITNTSGYFFQAYTGDGFVYHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDV1</accession>
    <name>A0A8H6FDV1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153602.1">
        <property type="nucleotide sequence ID" value="XM_037300785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDV1"/>
    <dbReference type="GeneID" id="59338321"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="449" mass="50279" checksum="35752FBE33A0CB32" modified="2022-01-19" version="1">MQSQSLAMAKPHLLSLPTEIIQSIYLHCLSPHLPLASPILATALSTQHIYKLTFLHAFWNRTPLVYAGAQVQQAYEPGPHVQDLFCPLPVPSGETDDAHARTERQEAVLGYRWCTFHRAKRYLSEAMSAVMKDLLLTLHLPLLPSDQTRLDAFIAQLPTRRCTFSATALDGSTIALEYPRRLATALRMTYDGHDPRFDTRATPNLTVSPTHVLCLPSHILTGHPTWTDSRIDFVQMVCSHVNLRTVQHDPSALSEGMRNAIVQGNHEALLVLIWHASRFAEYQETASHDDRPFEPPAELFRLVAKRGGPEVATLDQCPGSEAVNNATVSVRLFALLLRAHAESMPRDDPGITAWAHRLLTCQGSSKNDRAFAQWVLDWGSDGRSDEYINERYTLRRLEVEKVRRPMFRGGCVSRKRKGDEMAERFVEMCGGRIESFGDEVEAVEGAKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDV5</accession>
    <name>A0A8H6FDV5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DJ-1/PfpI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153617.1">
        <property type="nucleotide sequence ID" value="XM_037300800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDV5"/>
    <dbReference type="GeneID" id="59338336"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052158">
        <property type="entry name" value="INH-QAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43130">
        <property type="entry name" value="ARAC-FAMILY TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43130:SF7">
        <property type="entry name" value="DJ-1_PFPI DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="1">
        <location>
            <begin position="69"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="248" mass="26485" checksum="ADDD2C8E1AD3E71B" modified="2022-01-19" version="1">MASKPTIRIGVLLIDTVELLDLSPIDLFGMLSEPYVSTLSFLPAPLKAGAVPMEILYINQAGPNTLQECTANVGLRVDASISDKICAPAKKGGEKTLDILLIPGPDPSAYKPTDALNGFIEGHFDNGTDVLSVCTGIYPTGCAGVLKGKRVTGPRPLLPELQKKFPEAIWETKRWVSDGNLWSSGAFTNGQDMVAAYIREKWPGPTAEAMMALADVGERSQEYGVATVLRLSNIKDVASTCLLLPPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDY5</accession>
    <name>A0A8H6FDY5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153814.1">
        <property type="nucleotide sequence ID" value="XM_037300997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDY5"/>
    <dbReference type="GeneID" id="59338533"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="353"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="977"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1113"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="563"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="378"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="977"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1010"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1045"/>
            <end position="1059"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1220" mass="136704" checksum="1F81ED01B8238315" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEB4</accession>
    <name>A0A8H6FEB4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010427</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154097.1">
        <property type="nucleotide sequence ID" value="XM_037301280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEB4"/>
    <dbReference type="GeneID" id="59338818"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="603"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="643" mass="71656" checksum="CFF5070FB67BEFE7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEK5</accession>
    <name>A0A8H6FEK5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153841.1">
        <property type="nucleotide sequence ID" value="XM_037301024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEK5"/>
    <dbReference type="GeneID" id="59338561"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="397" mass="44821" checksum="D8806115F7156269" modified="2022-01-19" version="1">MAKTWPVSLLPLEKVTDKQLQILGEILWSWILCEECRTGRSCTTQACPSQRFKRLGRFFEHYKGLTASYEADAAPGDCAALSTHEDLFAIIKQLKLNPDITRAEFAAKVFRDDTSRRLPSSADRERAINLAVKVIAMVNCSAQRQSSGLLEHGLSQAPWRSDVTLAQFLVDIFPMTDHPSLNDDDLKGSMDLKAALMAKKLKKRIGLKLLPTDDLRRHLKLDRKTRVVEIYHHTAFLKEHLRLTKDKAPNMSVSESIRFGALPRQLALECLDSVQKILFPLTDRKSKSLLLSLISTSSFDPDCLRFDSASIRNNDEKDIAYHYFGEHLAELYDELENPTPRGSFEKWLERRSGARYVMLATLIGVIFAVILGMAGLAVGGYQAWVGYQQWQHPVSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEN4</accession>
    <name>A0A8H6FEN4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154421.1">
        <property type="nucleotide sequence ID" value="XM_037300573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEN4"/>
    <dbReference type="GeneID" id="59338108"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="11"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="312" mass="34436" checksum="C2557A9EECC07CA0" modified="2022-01-19" version="1">MAKTNVIDPEGDVILICGKTEFQVSSKVLSLASPVFKALFSPSFAEGQPTSSKASRIQLHDDDAESMLFMCAVLHHKCAFSKGIGLERLERLAVVTDKYDVSYAFDEAQFFNSLTKFMMLNLPNDGKAQTASRKYGIPEEIESHIPEGLIEAIFLAEQDVKREMQIQIEQLISPIAEKSTAGDLILGSDDIIFSHCDCISISSLINGLSRQGLWPLTSAVHRLSISMLCKKLEACDVGLASRITKLKKCSRCPENVAAKVAKIREAALTRFEGLCLDCIKNPGKSPEQRLQCRVPHQHFRGLPRQLPVSEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEU5</accession>
    <name>A0A8H6FEU5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial distribution and morphology protein 34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MDM34</name>
        <name evidence="13" type="ORF">HO133_010470</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. MDM34 is required for the interaction of the ER-resident membrane protein MMM1 and the outer mitochondrial membrane-resident beta-barrel protein MDM10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="10">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">Lacks alpha-helical transmembrane segments, suggesting that it resides in the membrane via beta-sheet conformations similar to those predicted for other outer membrane proteins and porin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MDM34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEU5"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21673">
        <property type="entry name" value="SMP_Mdm34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03105">
        <property type="entry name" value="Mdm34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027536">
        <property type="entry name" value="MDM34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058825">
        <property type="entry name" value="MDM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26545">
        <property type="entry name" value="Mdm34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="12">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="335"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="396"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="516"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6225273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="584" mass="63756" checksum="46CDA32AF3C7ECD7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF70</accession>
    <name>A0A8H6FF70_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154600.1">
        <property type="nucleotide sequence ID" value="XM_037300752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF70"/>
    <dbReference type="GeneID" id="59338288"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="73"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="230" mass="25869" checksum="7C8AA378EA0D1048" modified="2022-01-19" version="1">MAFTLQPVGPADVSDITRIFQSAFANDHIMSHFHPRTPDRLKWDQDFQFFSTQIAESAAYGGRLTKVVEESSGKTVAFSKWDYPHALTAEQRAEKKRKAMEDRLKKVVEGSNEGLMKDFFTQLIAGRERWLVPEKTFFLHILAVDPAYQRRGLGSMLIRPGLEDADKAGAQTYIEASPDGLPLYLKHGWEPVDKMVIDMGTHEAGSGVEVMPFLMREANAPNKVGKKIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGD6</accession>
    <name>A0A8H6FGD6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155608.1">
        <property type="nucleotide sequence ID" value="XM_037299607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGD6"/>
    <dbReference type="GeneID" id="59337138"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="78" mass="8091" checksum="782D21B0CDF874CE" modified="2022-01-19" version="1">MLAENLHPLLLQLQFPGARTGSAGKQENPAPNSSAQQKQSPKLSPSPNKALKLSLGAGMDPAGLPENPVPSKSVHLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGV0</accession>
    <name>A0A8H6FGV0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007795</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156001.1">
        <property type="nucleotide sequence ID" value="XM_037298665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGV0"/>
    <dbReference type="GeneID" id="59336192"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="227"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="251"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="280" mass="31308" checksum="6F18EE49E767A160" modified="2022-01-19" version="1">MAAEQRKLLEQLMGAGASSRHAQLSITDPKVCRSYIVGTCPHDLFTNTKQDLGPCPKVHSEPLKSEYETSPDKSKYGFEYDYMRDMQKYIDECNRRIDAAQRRLEKTPDEIRQTNDLLKQISDLTHSINTGLLEIAILGESSSIALAVLEHHKIRTAKAAKEEKERELKSLSDTSGPSGHQKLQVCDVCGAYLSRLDNDRRLADHFYGKMHLGYAQMRKTWEGLQKELKGRAPPQREEADRGPPRDEGWGGDRGGFGGGRGGFRGGGGFRGGGFRGRGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIQ1</accession>
    <name>A0A8H6FIQ1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007334</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156860.1">
        <property type="nucleotide sequence ID" value="XM_037298225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIQ1"/>
    <dbReference type="GeneID" id="59335733"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02231">
        <property type="entry name" value="cupin_BLL6423-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047142">
        <property type="entry name" value="OryJ/VirC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36156:SF2">
        <property type="entry name" value="CUPIN TYPE-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36156">
        <property type="entry name" value="SLR2101 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="225" mass="25029" checksum="90D334F34C9DFED1" modified="2022-01-19" version="1">MTALDIHSLGGGDIRTPHTVEVLPRVVRHATGRNAEGKSVFLSTDDDDHHHRELVYKSSIATINYSTYQHPAELNGDVDIEYAHENEPDISVENGSACRMVDFAPGGASPMQRANSLDCTVVIEGVFRMILDSGEERAMQRGDIAVQQSTTHRWVNVTGNGLLPARILVVPLEINDPHAGGEKVQRKLGLPRRDAVWLPSHKTEDGPDRGRFAKFHENDYWESEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIV6</accession>
    <name>A0A8H6FIV6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Spliceosomal protein DIB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007442</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Essential role in pre-mRNA splicing. Also essential for entry into mitosis (G2/M progression) as well as for chromosome segregation during mitosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DIM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156968.1">
        <property type="nucleotide sequence ID" value="XM_037298333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIV6"/>
    <dbReference type="GeneID" id="59335841"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02954">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000004">
        <property type="entry name" value="Spliceosomal protein DIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF5">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017199">
        <property type="entry name" value="mRNA_splic_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="143" mass="16937" checksum="279685D2982FD1A8" modified="2022-01-19" version="1">MGSVVLPHLVTGWHVDQAILSEEERLVVIRFGRDWDADCMRQDEVLYRIADRVKNFAVLYVCDLDQVPDFKQMYELYDPVTLMFFFRNKHMMCDFGTGNNNKLNWVLEDKQELIDIIETIYRGAKKGRGLVVSPKDYSTRYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ30</accession>
    <name>A0A8H6FJ30_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007561</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157087.1">
        <property type="nucleotide sequence ID" value="XM_037298452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ30"/>
    <dbReference type="GeneID" id="59335960"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="107"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="140"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="225"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="258"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="296"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6229445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="464" mass="49368" checksum="7DB0E2AB237CA366" modified="2022-01-19" version="1">MIACASDNASILERLLQAENMMLPTKDGRIIEHEILYQAASNGHKQIVETLLERSVQASASDTATGLTCLQAAAKNGHLQIVRALCTAGSLQKVPRGPDIDPPHAKMGMKALHYAAADGHDEIVAFLIQRGLECNERNLLGETALIKASQNGHAMVAKVLLEGGADPLLRGGEPWYTERPSSSPLAPIEEGYVFLTPVAFHHAASNGHANVLAILPYSDLNCGASETKALHLGAPYGHLDVVQALLFQGAKIESVGSRGMTALHHASRNGHNLVVKLQLDRGCRIDRRTDQGRPDQGRTALHFAAQAIDIKTIRGLVACGADINARTADPESYKSITAANSGAAFADLETIEVLLQGVGGRGCTALEEAIACNRRASVSALIELGAEWIRGEVFALAGIRADCEIVEMLLGKLSTATAKARVVAAAMFRMTLAEGASNRNEKAVRIFGDFARLNFKPIGSFNQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJW4</accession>
    <name>A0A8H6FJW4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004216</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157136.1">
        <property type="nucleotide sequence ID" value="XM_037295135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJW4"/>
    <dbReference type="GeneID" id="59332625"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14099">
        <property type="entry name" value="STKc_PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000652">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000004">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="67"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="476"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="605"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="660"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="875"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="878"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6229879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1058" mass="117314" checksum="4572D57D9F0C34DD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKC9</accession>
    <name>A0A8H6FKC9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dipeptidyl-peptidase V</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004493</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157411.1">
        <property type="nucleotide sequence ID" value="XM_037295410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKC9"/>
    <dbReference type="GeneID" id="59332901"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000028">
        <property type="entry name" value="S9 family peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF13">
        <property type="entry name" value="DIPEPTIDYL-PEPTIDASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="8">
        <location>
            <begin position="533"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6230154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="759" mass="83746" checksum="4833AE347EAB1F8A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKE1</accession>
    <name>A0A8H6FKE1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-hydroxyanthranilate 3,4-dioxygenase</fullName>
            <ecNumber evidence="9">1.13.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">3-hydroxyanthranilate oxygenase</fullName>
            <shortName evidence="9">3-HAO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">3-hydroxyanthranilic acid dioxygenase</fullName>
            <shortName evidence="9">HAD</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Biosynthesis of nicotinic acid protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BNA1</name>
        <name evidence="10" type="ORF">HO133_004476</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3-hydroxyanthranilate + O2 = (2Z,4Z)-2-amino-3-carboxymuconate 6-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:17953"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:77612"/>
            <dbReference type="EC" id="1.13.11.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the 3-HAO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.6" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKE1"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000334">
        <property type="term" value="F:3-hydroxyanthranilate 3,4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06123">
        <property type="entry name" value="cupin_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000093">
        <property type="entry name" value="3-hydroxyanthranilate 3,4-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00825">
        <property type="entry name" value="3_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010329">
        <property type="entry name" value="3hydroanth_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03037">
        <property type="entry name" value="anthran_nbaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497:SF1">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06052">
        <property type="entry name" value="3-HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6230137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="175" mass="19946" checksum="C03F01A8B620DE0C" modified="2022-01-19" version="1">MLSSPLNIPKWLEENSHLLKPPINNYCVYKDDVTVMIVGGPNARSDYHINETAEWFYQYRGSMLLKVVDDGNFRDIPIHEGEMFLLPPNTPHNPVRFADTVGIVLEQPRPAESKDRLRWYCQNCGDIVNEEAFHCTDLGTQIKEAVNGFKTSDEKRKCKKCGELADLVPRGVVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKI9</accession>
    <name>A0A8H6FKI9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157501.1">
        <property type="nucleotide sequence ID" value="XM_037295500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKI9"/>
    <dbReference type="GeneID" id="59332991"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="377" mass="40184" checksum="25CF9552185E0C32" modified="2022-01-19" version="1">MSLSSSSPSSHGKTIIWEEALPVRSSSESHASTIEPPTLSTTESHPGPRARSSTSPDGRLSASFSTRLSARKTQTPPLHLDLLSAAVRVSRSPGTLPTLAESPSRKPSAPIDIPARKKAEGQVSRPTTPLTARAPQGEYFTSWHKTEPRISESLTPYYSSRAKKTSASRDPSAAQRGFAAMSPRSESSQCRPSSPLSPKMSVPLPSINSSQKARAPAQGYNVAGLPKYHPANFPSRDHSPAPPSPLSSRSVTLQPRSGRGSDAKQQLLQYRRDLINNTAKTSRSLLSSSVSSKPSPPRLNPLRSPGGLMTPLMLEGAGDYFGTSSLPAGFKEGDGREIVERQVQRKNERWKDPEARSGSVSPALSLSPTVSPAGGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKJ9</accession>
    <name>A0A8H6FKJ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004567</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157484.1">
        <property type="nucleotide sequence ID" value="XM_037295483.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59332974"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ABC_SMC1_euk"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028468">
        <property type="entry name" value="Smc1_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF12">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="13">
        <location>
            <begin position="549"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="78"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="756"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="200"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="782"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="848"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1072"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="756"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6230227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1261" mass="143712" checksum="D4DD1D847429351D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL49</accession>
    <name>A0A8H6FL49_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein BTN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004867</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the battenin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037295779.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59333273"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018460">
        <property type="entry name" value="Battenin_disease_Cln3_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF0">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015974">
        <property type="entry name" value="CLN3_BTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="218"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6230523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="450" mass="48808" checksum="00A6831DC0F3934B" modified="2022-01-19" version="1">MNPAPIPQEMGGFTEGGLTHQEWGAEPRVCSAFWLFGLINNVLYVIILSAALDLVGPSVPKAVVLLCDVIPSFLMKLCAPYFIHAISYPIRVLLFSVFSAVGMLLIALTPPYTDGGTITTKMAGVVLASMSSGGGELSFISLTHFYGPFSLASWGSGTGGAGLIGAGAYALATTTLGLSSKHTIFASAFLPLIMLVSFFIILPREPLIRQAATVSSRSELPHATGDEDDEGSDPRETQEDEGLLPQSSNLTGANSLRMSKNQSWGAAFLQNLRRSRSLFFPYMLPLLLVYIAEYTINQGVAPTLLFPLKDSPFRHYRAFYPTYNAIYQSGVFISRSSTPFLRIHNLYLPSLLQCLNLAILILQALFGFIPTVWIVFAIVFWEGLLGGAVYVNTFAEISDRVAKDEREFSLGAVTVSDSGGVCIAGFIGMALEVGLCRWQVSHGKDWCQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZT2</accession>
    <name>A0A8H6KZT2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157537.1">
        <property type="nucleotide sequence ID" value="XM_037295536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZT2"/>
    <dbReference type="GeneID" id="59333027"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040025">
        <property type="entry name" value="Znf622/Rei1/Reh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182:SF8">
        <property type="entry name" value="CYTOPLASMIC 60S SUBUNIT BIOGENESIS FACTOR ZNF622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182">
        <property type="entry name" value="ZINC FINGER PROTEIN 622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="340" mass="38696" checksum="9251C268C545F1CB" modified="2022-01-19" version="1">MSVAFTCNTCKLEFESSELQRAHMHTDYHGYNLKRKIASLAPISAEIFERNATKAQDEANSTIEGGEAKGPDSLQTQPHRLEIERGYTPARCLFCNIDSSTMGDNLEHMFKVHGMFIPNQEDLEDAEGLLSYLFNIISGFRECLYCGKVKETVEGIRSHMIDKRHCKIAFDEELDQFYDFQGGSEDGAEEEMIREDEEHAEADKASPPRGHDFHPDSDHELRLPSGRTLGHRSLSRYYRQNLHSYPTPTERADRRAIIAAEAADGDHPMPDHPGRQLATRARGEMGMVGVSDFEKRALRATEKKALKQEARARNEHEWRVNKESNHQKHYRPADPGRTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZX3</accession>
    <name>A0A8H6KZX3_9LECA</name>
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        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004726</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157641.1">
        <property type="nucleotide sequence ID" value="XM_037295640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZX3"/>
    <dbReference type="GeneID" id="59333132"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF30">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04702)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
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        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
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        <location>
            <begin position="409"/>
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        </location>
    </feature>
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        <location>
            <begin position="434"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="503"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
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            <begin position="56"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="550" mass="61128" checksum="FC0D7F3D78984A3C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C692</accession>
    <name>A0A8H6C692_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Lecanoromycetes</taxon>
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            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000028">
        <property type="protein sequence ID" value="KAF6217426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146861.1">
        <property type="nucleotide sequence ID" value="XM_037297792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C692"/>
    <dbReference type="GeneID" id="59335296"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="217" mass="24401" checksum="4BA76227C1D9A324" modified="2022-01-19" version="1">MTCLTFLIPISLIAEIGRNERVSGMKTRHSEPEESVVEGTSANPLRVQDVGFASPIQRGKSQDKTSKPRGLAPMRDKEVALDVTDLLDAKPQIVSGIPEESLTLLNSNSFSTRDQFGNGVDSEHEMRLTRQVYPDYRFDLADERRRWHSKELIMVVISSKGGEWVSLYFLRSFTGLSELLRRLVGQRQEQAGKRPVYHEQDGTIIGVQRDATAVTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C698</accession>
    <name>A0A8H6C698_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147024.1">
        <property type="nucleotide sequence ID" value="XM_037297588.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59335092"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="129"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="333"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="426"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="562" mass="60248" checksum="12EAC31AF005FD29" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7A7</accession>
    <name>A0A8H6C7A7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147698.1">
        <property type="nucleotide sequence ID" value="XM_037297124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7A7"/>
    <dbReference type="GeneID" id="59334626"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="410" mass="46781" checksum="400A25216E8BFCD2" modified="2022-01-19" version="1">MPPPNDHDASRSEDAKPSQTDEPMFPEDLFADWPEGFHPPTPSTPLPQRSFEEKLEQYPDDDFYRSLRESMAQVRAESQRKVEALGFSSWKEKESHDVEERRKWLKEYVKEHGHSPPPPVITEDQRIAAELQYQRSVKPKPVPCDCEEHLHPCFCPQKLLNYKSQGDDPSDFHFLQSLHPPELNVRIQESDTSNRIPDRWLEHVWGIPSQDRILRLPQWVKRDETVQRMIDIEEVDETIYHGDYDQNKNHPPPLRTVSLTPDFSAEENAEASRDFERHGEPNRHKNREESAAGRNPKQEPPRITTKVDNFHASLPIGPTTDLSESRSHLAAETTTQRSRRGRGRNTSAPGVNKTKRASRAQACGIKKRPASMKEAAGKATLSGRMRTRAQGVLSFLALDSSGRATTISGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7C3</accession>
    <name>A0A8H6C7C3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147624.1">
        <property type="nucleotide sequence ID" value="XM_037297050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7C3"/>
    <dbReference type="GeneID" id="59334552"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="170" mass="19083" checksum="2B0883D67A97FC4E" modified="2022-01-19" version="1">MGIYKKTDILVLFQLYLLGLAALGSNIIILPNPNLTSVSNWTLPQPLDVLPSDPFSYRVPQSSQVITFSRYSRTLKREDVLACLLEAALEVIKEINLDHDGPMDTEEIQASSGHSLLILHPNPRLTWRMWGTTITGISNFLNDFEFVDCDFEVEILGYSANFGSGLLVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7C6</accession>
    <name>A0A8H6C7C6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005672</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147760.1">
        <property type="nucleotide sequence ID" value="XM_037296579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7C6"/>
    <dbReference type="GeneID" id="59334077"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF36">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13820)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="462"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="539"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="112"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="582" mass="63403" checksum="A4D0A8DD827751CB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7E2</accession>
    <name>A0A8H6C7E2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147665.1">
        <property type="nucleotide sequence ID" value="XM_037297091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7E2"/>
    <dbReference type="GeneID" id="59334593"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="126" mass="14448" checksum="9AE329F48F7CACA0" modified="2022-01-19" version="1">MSTYRRFPQASSDYNQAKSKAITALLEMKTAMRQMTAEHVNFHSHFDDVQKKLQDMKKAYGSKELDPEVIRSTALDKYLSEGNLPKVTALGHFEVENVLKDFQLQKKKLDEIRKQTGELVESLLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7F8</accession>
    <name>A0A8H6C7F8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147684.1">
        <property type="nucleotide sequence ID" value="XM_037297110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7F8"/>
    <dbReference type="GeneID" id="59334612"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="52"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="362" mass="41054" checksum="E4E292F9D8993017" modified="2022-01-19" version="1">MDDPIVAQQLNELTYERQDIYDLFMNLKTLEQNCPLAQPSIEGSNTASLYPLGQLDVLPLEIIQNILQLLDLQTLALTQSVNQRSKLLVDSLPQHREIVTHAPNALRAMLSTGLAPHFTINHLYRALRAQECIECGLFGAYLYLLECRRYCWLCLAEASDALPVSTEFAIHSFGLPRSAIRQLPCMKSLPGRYNLDGFHGDTHKRRVALVNPKAAKEAGMKLHGTQEAMEAYADPYAVRRFPRAMDRDRKEGNRNATWLQGVRHMQNPSRVRGPPWSHHGHYEPQRFMAAIPFPTLDLSDGAIEWGLSCKGCRDGPPNDDVSRDWEAMYTKRGYLAHLDQCEWSKHLSASLKTDSSTMSERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7X4</accession>
    <name>A0A8H6C7X4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="2 12">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_005974</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 12">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="10 12">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="12">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="2 12"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148058.1">
        <property type="nucleotide sequence ID" value="XM_037296877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7X4"/>
    <dbReference type="GeneID" id="59334379"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000025">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 10 12" id="KW-0274">FAD</keyword>
    <keyword evidence="3 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 12" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="14">
        <location>
            <begin position="25"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="13">
        <location>
            <begin position="369"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="467"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="201"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="341"/>
            <end position="344"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="62"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6218623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="488" mass="51905" checksum="E626EB837C31C44C" modified="2022-01-19" version="1">MIRTLIPKSASSRGRGYATEASEHDLIIIGGGVAGYVAAIKAGQEGMKVACIEKRGVLGGTCLNVGCIPSKSLLNNSHMYHQILHDTKKRGIDVGDVKLNLKQMMAAKNTSVDGLTKGVEFLFKKNKVEYIKGTGAFSGENEVKVNLIDGGEQTLRGKNIFIATGSEATPFPGLTIDEKKVVTSTGAIALESVPKKMVVIGGGIIGLEMGSVWSRLGSEVTVVEFLGQIGGPGMDAEISKTTKKILEKQGMKFKLGTKVLKGDSSGEGVKLEVEAAKGGKAETLDADVVLVAIGRRPYTAGLGLENIGLEVDNKGRLIIDEQYRTKLPHIRVIGDCTFGPMLAHKAEEEGVAAVEFVKKGYGHVNYNAIPSVMYTHPEVAWVGQNEAELKEQGVKYKAGSFPFSANSRAKTNLETEGMVKFLADAETDRILGIHIVGPNAGEMIAEGVLAIEYGASSEDVGRTSHAHPTLAEAFKEAAMATYGKAIHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C850</accession>
    <name>A0A8H6C850_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005960</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148044.1">
        <property type="nucleotide sequence ID" value="XM_037296863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C850"/>
    <dbReference type="GeneID" id="59334365"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051414">
        <property type="entry name" value="Adenylate-forming_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439:SF2">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439">
        <property type="entry name" value="PHENYLACETATE-COENZYME A LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="105"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="4">
        <location>
            <begin position="596"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="5">
        <location>
            <begin position="703"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1073" mass="118387" checksum="B3F92C29BB346ECD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8E1</accession>
    <name>A0A8H6C8E1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148205.1">
        <property type="nucleotide sequence ID" value="XM_037296223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8E1"/>
    <dbReference type="GeneID" id="59333719"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="139" mass="15046" checksum="22EEC485AE19E4AB" modified="2022-01-19" version="1">MTGTRTPLRLRLCPRAFNSEDVVEEDAGQEEHTLPDGANPSQEEAPSGGASPAQTINSLHLADEGSGEALQYRFRVILRDGEGWITVEEYSFATSLPSLVEVTAEKNAGEGQLLFDIRLCAFEAALAHGTNTIHFFPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8J9</accession>
    <name>A0A8H6C8J9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_006007</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148091.1">
        <property type="nucleotide sequence ID" value="XM_037296910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8J9"/>
    <dbReference type="GeneID" id="59334412"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046165">
        <property type="term" value="P:alcohol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="99"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="415" mass="44944" checksum="4EE7565B9D468FBF" modified="2022-01-19" version="1">METISRRRHQSTLAQAVSAAQHVLNGTPKTDPRLAGVSVDPLRIVGKELKFLTKNIQQLLGSGHPMLDTVAKYYTHSEGKHIRPLLVLLMSQATAFTPRASRHPSIPTASTIDNPISPPAVLSDVNPSSPSNDPLSAGSIEAAYNSEGSDILPSQRRLAEITELIHTASLLHDDVIDNSVVRRSLPSANVSFGNKMAVLAGDFLLGRASVALARLRDAEVIELLATVIANLVEGEFMQLKNTALDETNPTWTEDTLSYYIQKTYLKSASLISKSCRAAALLGQNSPETVEAAYSYGKNLGLAFQLVDDMLDYTTTSESLGKPAGADLELGLATAPLLFAWRDNQQLGVLVGRKFAVDGDVQMARDLVARSDGLEQTRALAQDYADKAAYAISRFPDSEAKEGLLEMCVKTMKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8T2</accession>
    <name>A0A8H6C8T2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TAFII28-like protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005401</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148293.1">
        <property type="nucleotide sequence ID" value="XM_037296311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8T2"/>
    <dbReference type="GeneID" id="59333807"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08048">
        <property type="entry name" value="HFD_TAF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045127">
        <property type="entry name" value="TAF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006809">
        <property type="entry name" value="TAFII28_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04719">
        <property type="entry name" value="TAFII28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII28-like protein" evidence="7">
        <location>
            <begin position="164"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="343" mass="37415" checksum="5588F98C0C4B5A5F" modified="2022-01-19" version="1">MASPPYRSPATSPPYPPHTSLPNPKKRPSVNVPPYAPSLKRQKRSSIHSAASSAHPLRQTSFPPEESAILTGERSPSVDSEITNMTGKGSIAMTGGRRRKSKGKGKKAAVSVKSGAKEKTVDGREATGEVPDDDDEDDDEGGDDIIDGVQEEDREAEKKKLAVLIGAFNYDQEKRYECLRRIRLKKETVRKITNQTVSQSVPPNVITTINGYTKTFIGTLIERAREIQEQYASLETPPLTPPKVPIDPTDFSSEHPNNEIFQAASPMDALGQLSYAAAVNEHQETQKPKQRDHGPLLPDHFREALRRHKRDGEGGGTGLQGVSTGLGLPGSGSARLAGRRLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Z4</accession>
    <name>A0A8H6C8Z4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004962</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148572.1">
        <property type="nucleotide sequence ID" value="XM_037295874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8Z4"/>
    <dbReference type="GeneID" id="59333368"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034302521" evidence="2">
        <location>
            <begin position="28"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="463" mass="48938" checksum="D2254477E9107CD4" modified="2022-01-19" version="1">MFLPPVHSSSTLYLILSLVIALPFIDSSPIPKLDLDIPQAVNCTDLKASFDSSCWKTLNLEGYLNDPETGWNRTAAICTGDEDDAACCLPTEPWTTCYLRLVHGFPGTDCSEINAQDCSYDPSLAVDPRIAPYVAYTMKTIYAINDLFTTWFNALQYAASQALSIVQDVINQVDPPQQTNFILRDLLFALTVGLAYIVAPEAGSLTGLLVATAAAGRALLKGANQAPTVARGIWPAGTESSQSIQIGDVENELNNATGITAIMLNSGLELLMSDMPSFVEFASTGMFSGSDSLSLPKEVDGLDVALKTFIVSNAMTSNGWHYTVSLGATRDDISSCVPGSGGSCCTWWTLGSSSSSSSSSSSSSSSSSSSSPAPSMCGDFIWYSDATMRAFRLINSHNLNGTVDMLSAIVENAWTTLPLLFDGAYNCSAAGTFGGSPIQIEADGTIDLSCMSQLAEGPSLSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C959</accession>
    <name>A0A8H6C959_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005032</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148642.1">
        <property type="nucleotide sequence ID" value="XM_037295944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C959"/>
    <dbReference type="GeneID" id="59333438"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="41"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="356" mass="38851" checksum="538B82824807C1CF" modified="2022-01-19" version="1">MATLSPSEFAFQRSHIGDDRRTGITVSNVICYTLAVVAVLLRVFSRHLAQVEHRADDWWIWGALFLFTVYIAAYQVLVHFGLGRHVFLVTNMKGFVVTSIFGATAYNLTMLAIKLSIVYFYRRIFPQRWFKRALIGTGAIVVGTALAQVPLDIVPCMPIASQWDRNVKGKCIDLGAAILGIAISNIITDVILLTLPMPLLWNLKVSPSRRSLLLSLFLLGGCACIVSLVRVFFVQQAEGADPTWNYVNTAELSTVELGVGISSACLPTCRPLYNHCFHNSDPTRGGARSKAYGHSSRAIRMADIGRGWYGNREALGSGGDDGGLHAASAARGEWRDGGGGRILVTKEFASESVGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9D0</accession>
    <name>A0A8H6C9D0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148689.1">
        <property type="nucleotide sequence ID" value="XM_037295991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9D0"/>
    <dbReference type="GeneID" id="59333485"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053206">
        <property type="entry name" value="Dimeric_xanthone_biosynth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38048">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38048:SF2">
        <property type="entry name" value="HEMERYTHRIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="281" mass="32096" checksum="EFC3E12149040F87" modified="2022-01-19" version="1">MASGISSNKDKLLPLITTPAYATGKNDPFTIYASRITLSHNAFIRGFNSIYQQAPNLTPSDHKDFIGYCLAWYTCVGEDHSREEIRFFSAMEDATGEEGILDHEVTFYTIYRDDMQKLKNYLLSFEHFRNPETEFDVSRLLFLIDRIAHPLHTHLASVPQSILALSRFSTPERPIDPLKIDRSLDVPDEKKTTNYIFNVLPAWLLNLETEEFEGGRWKGWPESKLENQHHVDCCRGGSVGGVTLLAKPSSIIRAYEYSSFNTYQDNGAELYDWNAQNKGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Q5</accession>
    <name>A0A8H6C9Q5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148611.1">
        <property type="nucleotide sequence ID" value="XM_037295913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Q5"/>
    <dbReference type="GeneID" id="59333407"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="123" mass="14295" checksum="738153F731C205CA" modified="2022-01-19" version="1">MRTSDEIKAHLHHGVHELRQVQQLLATVSDRIQTVFDAIQACNARADDIDPTIDTLNRWSHTLIEEMEPKEALRDKIAALVKQDAEIQRQVTAAEASHFKDDNHKERVNAPGRRMHRYQQRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9W2</accession>
    <name>A0A8H6C9W2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005239</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148848.1">
        <property type="nucleotide sequence ID" value="XM_037296150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9W2"/>
    <dbReference type="GeneID" id="59333645"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000093">
        <property type="entry name" value="Fidgetin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF17">
        <property type="entry name" value="FIDGETIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="600"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="94"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="464"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="856" mass="93744" checksum="3D1D11CB8279028B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9W9</accession>
    <name>A0A8H6C9W9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005091</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148701.1">
        <property type="nucleotide sequence ID" value="XM_037296003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9W9"/>
    <dbReference type="GeneID" id="59333497"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040928">
        <property type="entry name" value="Importin_rep_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18816">
        <property type="entry name" value="Importin_rep_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="30"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="799"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1098" mass="121984" checksum="9C8CC9C18B980806" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAD6</accession>
    <name>A0A8H6CAD6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cns1/TTC4 wheel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003785</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TTC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149395.1">
        <property type="nucleotide sequence ID" value="XM_037294707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAD6"/>
    <dbReference type="GeneID" id="59332196"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21381">
        <property type="entry name" value="CTWD_TTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000611">
        <property type="entry name" value="TPR repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044059">
        <property type="entry name" value="Csn1/TTC4_wheel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18972">
        <property type="entry name" value="Wheel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Cns1/TTC4 wheel" evidence="5">
        <location>
            <begin position="320"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="435" mass="48558" checksum="86A81FFB94290723" modified="2022-01-19" version="1">MSRFEELPDNFHESLNLNPPPAPAHAPPSAPIVFPAASSDSTPFPIDASRLEQNGTTPALPPHMASVRSHTADEIVQMMNRTPLFMTSLESAEGEDGENVELDALRALQYEGTPAEIAQGFKEQGNEVVKMKRWKDGKEYYTKGLAVLSQRKKVRSLRSPEETGEKPSIAEDEAEIRKQKELEEVCYVNRALCNLELKNHRSTTLDCASALRLNPKNVKAYYRSSLALFALDKLAEASDACTRGLTIDPSNAALKRFATKIEARHKSLSAIEQKKREREQRAQKEKFMLKTALRARNIRMRNTAQPPEMEDASIHLSPDPIAPTSTLMFPVVLLYPLHLQSDFVKAFSETDTVPNHLEYIFPLPWDEKHEYTLGSVELYMETGSGGLIKVGKKVSLGKVLGNDGMEIVDGIVRINVLLKARAAGWIEELKVRRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB30</accession>
    <name>A0A8H6CB30_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MINDY deubiquitinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003751</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149361.1">
        <property type="nucleotide sequence ID" value="XM_037294673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB30"/>
    <dbReference type="GeneID" id="59332162"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016807">
        <property type="term" value="F:cysteine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007518">
        <property type="entry name" value="MINDY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033979">
        <property type="entry name" value="MINDY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063">
        <property type="entry name" value="NF-E2 INDUCIBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04424">
        <property type="entry name" value="MINDY_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MINDY deubiquitinase" evidence="2">
        <location>
            <begin position="358"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="663"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="736"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="665"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="692"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="775"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="838"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="858"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="920"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="931" mass="100727" checksum="4C90F0B1FA9E1DB6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB60</accession>
    <name>A0A8H6CB60_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003467</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149770.1">
        <property type="nucleotide sequence ID" value="XM_037294389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB60"/>
    <dbReference type="GeneID" id="59331878"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="319" mass="35924" checksum="830CD2EE4BC2B4F8" modified="2022-01-19" version="1">MSGLEIPGFIVGLSGRIAVAEKGLVIWRSIGVANVFGPNVVEDVAKLSMEYYRFEAWTNAVSFLLTAQKRSQSKPTALGMLSSFLEPAAPTTNIHDALLRQHYVMFRETHIYSVVKFCEYRQEFQQEMDIEPLTYLPTHDDVGSQRLQDDNHLYVPARRADGTILEGVRQGRYSIAMAHSFDWGIVLKAHRPGHTYIADDLRSGPLYMIGLITEDGWVASALKDSEQEWNNTNHLLALPSLKSRKQQFPVLVQIDVVARIHVWCSGKESAKELKAPRGTLNAVYLDGMRRESPTVWCNSETLQIGITCWRANPIVLPVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBA6</accession>
    <name>A0A8H6CBA6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Benzoate 4-monooxygenase bphA</fullName>
            <ecNumber evidence="12">1.14.14.92</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Benzoate-para-hydroxylase A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Cytochrome P450 monooxygenase cyp53A1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_003484</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>benzoate + reduced [NADPH--hemoprotein reductase] + O2 = 4-hydroxybenzoate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18033"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16150"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.92"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.92" evidence="12"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149787.1">
        <property type="nucleotide sequence ID" value="XM_037294406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBA6"/>
    <dbReference type="GeneID" id="59331895"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018664">
        <property type="term" value="F:benzoate 4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11061">
        <property type="entry name" value="CYP67-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000053">
        <property type="entry name" value="Cytochrome P450 benzoate 4-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF29">
        <property type="entry name" value="BENZOATE-PARA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 16 17" id="KW-0349">Heme</keyword>
    <keyword evidence="7 16 17" id="KW-0408">Iron</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6220352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="517" mass="58218" checksum="02EA489ABDC51F92" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBG1</accession>
    <name>A0A8H6CBG1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149797.1">
        <property type="nucleotide sequence ID" value="XM_037294416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBG1"/>
    <dbReference type="GeneID" id="59331905"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="339" mass="38558" checksum="45BBA2C9822682F8" modified="2022-01-19" version="1">MIAVAVKRDAKSLPNLASLVVPNGPLDQYKKEIEEMFPKLTATHGISDIAKRFNFTASKKDIQDILSIWEHYKTTCMICMNFDQAQLLLSLNKMPESISSPPVPRTYHQSRPLVTFPSDARSFVGRAKLLKALTEGLGECSRMALYVWSGWGRVRDLPSEHSDFVNYLTGVSDDELESDKDIGILVQYSLISRNENLVSYSTHRLVQLTSRIWLSGQGASGNWKAEALMAVLYAFTATLSVDEENLIWKWPETIIHTLALFQSPAINSRCIHKEHSSSLRLRTVSGTSGSRWLRTPKQRPPRWWLDKILRCTLRALVRPREVYKCQSMASERTKMLKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBL4</accession>
    <name>A0A8H6CBL4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149855.1">
        <property type="nucleotide sequence ID" value="XM_037293777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBL4"/>
    <dbReference type="GeneID" id="59331264"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034014324" evidence="1">
        <location>
            <begin position="16"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="285" mass="31500" checksum="3036431030BD7A1E" modified="2022-01-19" version="1">MFMKTFLLLALGVQALSLPGTDEALEKRSSRAWIGSFDSDDTTCSNTSVFQDNSALPHAACVPFRLQGGRVGGRWGSGKHEINVIQAFEDTGCQEAQISGSGYTDTEIDAYTDADSYAHTDGDTDTDTENSSIDDPWPKATIRLAHFSVKEYLVSDRIRHGSASDFGLQEIDSNESMAEYCLAYLLYIIKLEVLTSVSLSEYPLAQYAMAYWFEHVRIAPSNTSSALPLAQELYLSKKDSLVKWIRLYDADPPGHWGPIRILSSGEESQHIGLPMYYASLLGLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC73</accession>
    <name>A0A8H6CC73_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150237.1">
        <property type="nucleotide sequence ID" value="XM_037293408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC73"/>
    <dbReference type="GeneID" id="59330895"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="187" mass="20515" checksum="A416830B359A279B" modified="2022-01-19" version="1">MSIRILDHNGTALQLSARMSADFEAELRRIGEAPAPPTLNLWVSNQTPDELLQILKRMPGRPADESARGGVQELQSEISVPEDSDRDTAAGHQVGDPKSDIQAVGLPSKPDYAAGTGTLWASGGVYNSVYQRETGHIMADPGGLGELVVRDGKMVNLRDEQREELGKAKEREDLERERQERKDGESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC79</accession>
    <name>A0A8H6CC79_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002449</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150204.1">
        <property type="nucleotide sequence ID" value="XM_037293375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC79"/>
    <dbReference type="GeneID" id="59330862"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050493">
        <property type="entry name" value="FAD-dep_Monooxygenase_BioMet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789:SF309">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_6G14510)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="145" mass="15571" checksum="6C6F34F5283AEB05" modified="2022-01-19" version="1">MPPSSRSLPTSGSGAGGNNRKPQQPQLHQQLMQVTLESGIIGKSGLKVESMDVTKTAILLADGEAISTDVINAADSLHSVVRSHILDSKKSFPQRSTGQSAIRFMLPEAVTQRDSILSTAVSDDVLMLSWKGNDKRVLVPVDYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC82</accession>
    <name>A0A8H6CC82_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_002497</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150252.1">
        <property type="nucleotide sequence ID" value="XM_037293423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC82"/>
    <dbReference type="GeneID" id="59330910"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="36"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="400" mass="43942" checksum="A26F46D25AA658C3" modified="2022-01-19" version="1">MADTPPPGIDLNADQGPRLVGSMITLIVLPTLFVIARLVSRKVARAGYWWDDFFVVLACALCYIQCICVLISERRNDFGKHIYGLKDPADNTVEFLKILYIYLIGYFSATSVVKLAILAFYRRIFPVQQLKLLLFIAVGIVLCYFLGSLLTAIFQCQPIHHFWERAGPGKCANGDLVQIVPGAINCVIDFFIIMLPIPLLWRLRTTASQKGVLTGIFVCAGFVCIISIIRLVVLSRLAAVDVTSQPGNYVDSAIWSAAEPSMGVISACIPSLRPLISLLARGTTRGVGVSKTPRSKPSAQDTTSSVASRIAWRSPRVGPEDCREGYFERLEDPSEAVSPGGARPGFFRHEANVRGGKVVGRGREDEISLEEMNVPAGGIRVKDEVVVTSSDWLEYKDKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCB2</accession>
    <name>A0A8H6CCB2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150138.1">
        <property type="nucleotide sequence ID" value="XM_037294060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCB2"/>
    <dbReference type="GeneID" id="59331548"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="791"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="870"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1003" mass="112526" checksum="D4CA438A21DD397C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCI4</accession>
    <name>A0A8H6CCI4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150432.1">
        <property type="nucleotide sequence ID" value="XM_037293603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCI4"/>
    <dbReference type="GeneID" id="59331090"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="326" mass="37654" checksum="77BC52D596D14913" modified="2022-01-19" version="1">MSTIQATDNAYESFFTRYAYLCKLAANKDREIERVTHWHLVEIIALLKDHGSTREEIAVMQNPVIAHSESLARTLVDLAAGLWLMLLISKYPGDISYDEPIIWGDNEKLGEEQHELNEGLINKNFSYRYNSTDLVKLPQAFTAAHLEQIGGIKVEWTSNLADHLLLKDDDTKLMLFHQVSILQLHKKSPTTLLPKVLVDETIRSISLLIPPVLGKPNPWFQQQRKKWLIDAQAGVCHRLNSSERQIEKFVYWRERLVLLKRTFDDAEPRNISQLWWDDRKKTQWFTFWVAVLVFIMTVFFGVVQSVAGIVQAWASVQSLKAQKAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD97</accession>
    <name>A0A8H6CD97_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150501.1">
        <property type="nucleotide sequence ID" value="XM_037293672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD97"/>
    <dbReference type="GeneID" id="59331159"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="59"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="426" mass="48017" checksum="DC4824E1F21AEF00" modified="2022-01-19" version="1">MSPSMFRVQEHTIPCQYVREYPGATLENEEDDLVLHVKQYTPLDQSYTQAGAITIIGTHANGFPKELYEPLWDDLSQALRKQGQTIRGIWIADVAQQGMSGVLNEDKLGSDPSWNDHSRDLLHMINHFRKEMSRPLVGVGHSMGGNQLVNLAYLHPRLLTSLVLIDPVIQTASAAAPDKSRLSHTQASTFRRDDWPSRVEAASSVKKSKFYQSWDPRVLDLWIKYGLRSLPTAIYPDRSENDDDPVTLTTTKHQEVFTFLRPNFDGLDANGKLVVNRPTHPDLDLRAADTYPFYRPEPPAVFNDLPRLRPSALYIFGGQSDLSTPDLRKQKMERTGTGVGGSGGAEADRVKEVVLEDVGHLVPMEAVARCATAAGEWLASDLKRWRSEEADFKREWEAKERKAKCTISEEWKKNIGGDPRRKSEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDB1</accession>
    <name>A0A8H6CDB1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl hydrolase family 13 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150596.1">
        <property type="nucleotide sequence ID" value="XM_037292943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDB1"/>
    <dbReference type="GeneID" id="59330429"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="beta-trefoil_Ricin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14200">
        <property type="entry name" value="RicinB_lectin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="2">
        <location>
            <begin position="181"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="611" mass="65304" checksum="D3D422DB5F024E8B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDY8</accession>
    <name>A0A8H6CDY8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150919.1">
        <property type="nucleotide sequence ID" value="XM_037293266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDY8"/>
    <dbReference type="GeneID" id="59330753"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="40"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="300" mass="34112" checksum="B28B4DBD2982F6A5" modified="2022-01-19" version="1">MPSQNTPIPHHRQIWLQQADPEIRIHYIEYLPTSSEPKGTILLIHGFPETSYQFRHVTIPLAEAGYRVIVPDYRGAGYSSHPPDGYTKDVLAQDLYKLVTEHVGVKEPIHVVGHDIGGMIAHAYVAQFPSNVASVIWGECPLPGTKFYEDNKHTAPLWHFDFQAQSDIAVSLVSGKEKIYLKHFYDRLAQNPEAFSSETLEFYATQYSMPGALRSGFHVYRMFETDAVHNQEWLKKNGKVEVRSMILSGEHVFMTASAFEMASEMYSNVERGIVEGSGHWLAEENPEDFVRKVLGFVEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE15</accession>
    <name>A0A8H6CE15_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]</fullName>
            <ecNumber evidence="8">1.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Saccharopine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_002327</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-saccharopine + NADP(+) + H2O = (S)-2-amino-6-oxohexanoate + L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.10" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150906.1">
        <property type="nucleotide sequence ID" value="XM_037293253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE15"/>
    <dbReference type="GeneID" id="59330740"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004755">
        <property type="term" value="F:saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000008">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000072">
        <property type="entry name" value="Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1870.10:FF:000002">
        <property type="entry name" value="Saccharopine dehydrogenase Lys9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1870.10">
        <property type="entry name" value="Domain 3, Saccharopine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="11">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="124"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6221471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="450" mass="49112" checksum="E509D3BD00419814" modified="2022-01-19" version="1">MAPQSVLMLGSGFVTKPTLDILSDAGIEVSVACRNIDSAKKLSEGVKLAKPISLDVTDDKTLDAAVAQHDLVISLIPYNFHPTVIKSAIRNKKNVVTTSYVSPAMLELEQQAKDAGITVLNEIGLDPGIDHLYAVSMIESVHKAGGKIISFLSYCGGLPAPEDSGNPLGYKFSWSSRGVLLALRNAASFYQGGKVVNVAGPELMATAKPYHIYPGFAFVAYPNRDSTTYKERYNIPEAKTIIRGTLRYQGFPEFVKVLVDIGFLSNEEQSFLKEPITWKEATQKILGASSSSEKDLLWAISSKTTFKDNDEKNQLMTGLKWVGIFSDSKITPQGTPLDTLCATLEEKMQFEEGERDLVLLQHKFEIENEDGRKETRTSTLCEYGAPIGTGGYSAMAKLVGVPCGVAVKQVLNGTISDKGILAPMNSKINDPLMKELKEKYGIECKEKIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE35</accession>
    <name>A0A8H6CE35_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151212.1">
        <property type="nucleotide sequence ID" value="XM_037292673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE35"/>
    <dbReference type="GeneID" id="59330159"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF97">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G10890)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="272"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="481"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="780" mass="84157" checksum="495E3DE906F035A8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE53</accession>
    <name>A0A8H6CE53_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Malic acid transport protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="ORF">HO133_001893</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151360.1">
        <property type="nucleotide sequence ID" value="XM_037292821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE53"/>
    <dbReference type="GeneID" id="59330307"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015140">
        <property type="term" value="F:malate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09317">
        <property type="entry name" value="TDT_Mae1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030185">
        <property type="entry name" value="Mae1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31162">
        <property type="entry name" value="MALIC ACID TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31162:SF3">
        <property type="entry name" value="TRANSPORTER_MALIC ACID TRANSPORT PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="51619" checksum="0451915FA7AD9C67" modified="2022-01-19" version="1">MHIPTHMLSPGQNGYRAPTVEDYHVQNGGSTTPNSARTPNGVAPPNDHSTPNSLHHRLPHIRDQVNLSTTSLARSILDELHWGERIRHYTWTFFTMTMATGGISNVLYAVPFRFRGLEAIGIIFFLLNIVLFIFNIVMISLRFYFHPETFKASFLHPTERLFIPAAVVSFGTILLNISQYGPSHAGDWLNDVVVVLFWVDAGLALTASMGVYLIMWSTASFTIENMTPIWIFPAYPLLIIGPHAAVLSQTLEQRRAFDIIVGGFTLQGIGFMVSLMIYSAFIYRLMTQRLPQASTRPGMFVSVGPCGFTVAGVIGMAEGAQRALPPDFMGNTPMSAMILKVISAWMSLWIWGLAFWFFFISAGAHWSCVGPHKMVFTMTWYSYVFPNTALVTSTFAIGKAFNSRAIQIIGCVMTAILILTWFFVVGMMVRAIMLKQILWPQKGEDKDEGGFKAPRPNRRISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEQ3</accession>
    <name>A0A8H6CEQ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-NAD kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001296</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151645.1">
        <property type="nucleotide sequence ID" value="XM_037292226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEQ3"/>
    <dbReference type="GeneID" id="59329712"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000029">
        <property type="entry name" value="NAD+ kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000008">
        <property type="entry name" value="Putative NAD+ kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF11">
        <property type="entry name" value="KINASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G12870)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="58455" checksum="931C7B8BA4CB7C6E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEW3</accession>
    <name>A0A8H6CEW3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001298</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151647.1">
        <property type="nucleotide sequence ID" value="XM_037292228.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59329714"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="306"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="665"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="778"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="849"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1171"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1312"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1429"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="612"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="804"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="891"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1171"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1318"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1431"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1449"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1459"/>
            <end position="1477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1491" mass="164766" checksum="0BDB7C3142A7A3E6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF17</accession>
    <name>A0A8H6CF17_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151810.1">
        <property type="nucleotide sequence ID" value="XM_037292391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF17"/>
    <dbReference type="GeneID" id="59329877"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="189" mass="20924" checksum="4C0671043CCDBB06" modified="2022-01-19" version="1">MFYLLCPYTAAAQAASCDVSTYGKPEKRDCLTLFEKFTSSQNLQTRFFDEEQLRVDSDSTWPGVANVFEQPIVQLPRFYAMNTCNFALMPYTNPATRLANPLDISSWGIIKSYGNSLMQDCLEEKSAGGEIFIKSSTSMNPVLVIFMWASGANFETKLNQYENDPSFSPQLSLAEGGVNGTDGDGIRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF72</accession>
    <name>A0A8H6CF72_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-soluble NSF attachment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001463</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151812.1">
        <property type="nucleotide sequence ID" value="XM_037292393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF72"/>
    <dbReference type="GeneID" id="59329879"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000049">
        <property type="entry name" value="Alpha-soluble NSF attachment protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF8">
        <property type="entry name" value="ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="33188" checksum="D0C1B6EE387DED99" modified="2022-01-19" version="1">MVMMAQDPVTLASQADKAAQSAQSGFSFFGARTDKYESAVELYTSAASAYKLQKNYREAGSLYEKAAMIQKTKLNEPDDMANTLQEAFKAYRKTAPEEAARVLSLAIDHYLSKGNFRRAAVHKQNLAELYEELSDRRRAREAYEAAATWYDDDNASALANKLYLKAGDLAALDEDYLPAIQYFEQVARQSISNNLMRYSVKDYLLRAGICHLAFDIVGAKRALESYRDLDPSFLAQKEYQLLNDLGEAVEHSDAEVFAEKLFRYDQLSPLNSWCTTILLRIKNKITDHEDDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFD6</accession>
    <name>A0A8H6CFD6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001260</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151609.1">
        <property type="nucleotide sequence ID" value="XM_037292190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFD6"/>
    <dbReference type="GeneID" id="59329676"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12236">
        <property type="entry name" value="RRM_snRNP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000298">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034143">
        <property type="entry name" value="snRNP70_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022023">
        <property type="entry name" value="U1snRNP70_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF5">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12220">
        <property type="entry name" value="U1snRNP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="97"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="354"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="421" mass="44668" checksum="1FD28933413FB36E" modified="2022-01-19" version="1">MTHKLPPNLLALFAARPALRYLPPNDHAPEERKTNNIGGVAQYISAVEDYKENISYEPTESWLQRKDRVKLEKKEKQEQLIKEEPGDDPNVRGDAFKTLFVARLSYDTTEKDLEREFGRFGPIERIRIVTDTSQAEENGKSKKKKKGHRGYAFVVYEREKDMKAAYKETDGIRIKDRRVLVDVERGRTVKGWRPRRLGGGLGGRGYTKAMPARPAGVGGSLAAPAGPGGFGGGFRGGFGGAPRGGGFRGGFRGGDRGGFGGGRGGIGYQGGNGFGGGRGGYGGGPPNGYGNAQAAPPNAPSGPGGNRGGFGSGHGSPPVNGGMNGTPTPVDGPRGGYGGGESRRNDFDDRSSYRGGGGGGYGDGRSGGITGSNREPVRPRDGGGYGDRDRDRSYGGGGGGRDDRKRQYDGDGYDDPRKRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFP6</accession>
    <name>A0A8H6CFP6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001472</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151821.1">
        <property type="nucleotide sequence ID" value="XM_037292402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFP6"/>
    <dbReference type="GeneID" id="59329888"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF50">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="574"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="697" mass="79065" checksum="87ABA56CABEC3037" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFY7</accession>
    <name>A0A8H6CFY7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000919</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152214.1">
        <property type="nucleotide sequence ID" value="XM_037291852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFY7"/>
    <dbReference type="GeneID" id="59329337"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF32">
        <property type="entry name" value="FAD-LINKED OXIDOREDUCTASE AFOF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="342" mass="36926" checksum="19A542C94F65DAC1" modified="2022-01-19" version="1">MVGATIGAGVGPLEGLHGLVLDALVSVRMVTASGQLVTASDDENGDLFWAVRGAGANFGIITSATYRVHNHTNGGQAVNADFIFPPAANVSLFEVLKSWDDDDVFPQNLGLEIAASFNHTSQLPNLDANFIFYGTQEAAQPYLDKLIALNPLRYQNLTVGWNNLTNVAGFGHASGVCARGDYTTHYSLGANRTDVATFSAFFDNFTAFSAANPWFDGSFALERYNTKAALAVPESERGVYPWRDIKTLVIFENVYPNSSHDAAVAAFYGPVRRAFQAVSGFAVPHVYVNYGYGDEGPAAWYGAHNLPRLSALKRKWDPRGYFGAGNPVPLSWEGEGEGALIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGF1</accession>
    <name>A0A8H6CGF1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000784</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152082.1">
        <property type="nucleotide sequence ID" value="XM_037291720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGF1"/>
    <dbReference type="GeneID" id="59329202"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023041">
        <property type="entry name" value="Glucose_rcpt_Git3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11710">
        <property type="entry name" value="Git3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="6">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="341" mass="38350" checksum="96B115DD67D25C21" modified="2022-01-19" version="1">MSESPIRAGADSHLCQFQGFLIQMFVPADALWNLAMAINVYLTLFKKYNAQQLKALEWKYHLMAYGAPFVIAFIFIFVENEERGKIYGPAVLWCWLSHDWDFLRIAVCYGPAWVCIIASFAIYIMAGREIFKKRHQLRAFSNPSDDHNYAYKTTDYQVTSDFESLPLKNISEAFISPADRKSSNPSDPSHVSYPKYNVKISAAPRPSILHHTSIEYKQQKKNRAALEANRAAFGYTKVASLFFVSLLVTWVPSSINRVYSLIYPESISVPYAYAAGIVLSLMGFWNSVIYVTTSRAACRTLFLNLFQKFGGSRSARLSIDGDGPLMGTAYESPPREGAAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH21</accession>
    <name>A0A8H6CH21_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000067</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152442.1">
        <property type="nucleotide sequence ID" value="XM_037291007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH21"/>
    <dbReference type="GeneID" id="59328486"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="40036" checksum="4377D4015C38A5CE" modified="2022-01-19" version="1">MTPAPAPATTHPPNPTFPPHPAPRNWLITACTSPIGIAIARQVLSHGDNVIAGLNPRHLAEGHGDGGEEEGDAQRGEEFREFWRECEEEGWKERCKGVELDGSVMGLCQAAVAEAVEVFGKLDILLCCASEALVGTVEELAASPRTQTLVREQFESNFFGPVNCIKAALPTMRRRNSGHVMILTGITGHLGTPGLGMYCAAGWALEGFCDSLAYEIAPFNIKMTIVQPTMEIGVLTNKIISAPSMPQYSPDNNPAPLFRGIIGGLLDRLDLVQQNGNYEPASLAGDRIQTLYPKLPPEMQSSLLAETINAITAIGGHDNPPARHIVGHEGVASVKEKLKTVSEELEDFVDCSCAVDIDDGHGIEDANGSIDGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH99</accession>
    <name>A0A8H6CH99_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enhancer of polycomb-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_000330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Involved in gene silencing by neighboring heterochromatin, blockage of the silencing spreading along the chromosome, and required for cell cycle progression through G2/M.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the enhancer of polycomb family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152704.1">
        <property type="nucleotide sequence ID" value="XM_037291269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH99"/>
    <dbReference type="GeneID" id="59328749"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Enhancer of polycomb-like N-terminal" evidence="9">
        <location>
            <begin position="13"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="529"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6223487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="567" mass="64191" checksum="161450BC2DD4545F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHL4</accession>
    <name>A0A8H6CHL4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152854.1">
        <property type="nucleotide sequence ID" value="XM_037291419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHL4"/>
    <dbReference type="GeneID" id="59328899"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="185" mass="20114" checksum="73E8EC8E143D053C" modified="2022-01-19" version="1">MFGSTKPLRTLNVYDKGSTYTILDTDDATLLYTVRWNANSAPHMTVFHADDRNKIAGTATYHATKKFGFSTASNITLKLSSGTVSMNKEGGLFSTDKRTLRSAGLGEVYWKGGYSETGFMKLVDGNGKSLVEYKDMRMSMKTMGILEINVELGQEALDEVVVSGIAMLSEERTSMSATGAAMSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI03</accession>
    <name>A0A8H6CI03_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152907.1">
        <property type="nucleotide sequence ID" value="XM_037291472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI03"/>
    <dbReference type="GeneID" id="59328952"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="538" mass="60115" checksum="5DA1317B45A4FF78" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI45</accession>
    <name>A0A8H6CI45_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152861.1">
        <property type="nucleotide sequence ID" value="XM_037291426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI45"/>
    <dbReference type="GeneID" id="59328906"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="685"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="418"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="725"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="748" mass="83836" checksum="C987C5D270C38093" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CID8</accession>
    <name>A0A8H6CID8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010702</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAF13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153188.1">
        <property type="nucleotide sequence ID" value="XM_037301554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CID8"/>
    <dbReference type="GeneID" id="59339092"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07978">
        <property type="entry name" value="HFD_TAF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF5">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="225"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="280" mass="30784" checksum="583730642DC9FFFA" modified="2022-01-19" version="1">MARGKKRSRADRKAPMTDMPAPKALTFGNSSSRPEFQTDNSSPGEVLDPLSSHHTLRRPAAIAASSAIAKAALYHDESDEEVETKRRNKKVTVIEETKAIIPDGYAPTSHPSAPAGSPNVKMTEVRARAARHKGQMNFEAEITQALYAYGDDKEPLPETVRILDEMATDFIIETCFGAAKSAECCGRSKLKVDDFKFAIRGDPVLLGRVNELLAMDKHINTARKAFKTDEGREGLERGGRKKKGEGKDGEEKVEGKAEDKEVKVEDKVEEDLGDDLDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIP9</accession>
    <name>A0A8H6CIP9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010782</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153267.1">
        <property type="nucleotide sequence ID" value="XM_037301633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIP9"/>
    <dbReference type="GeneID" id="59339172"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="376" mass="41272" checksum="0B2F0BB2A0705AD4" modified="2022-01-19" version="1">MVVDIPGLEDGTVAELLKAEDEDADPDPDPDDEDELLPRDNAATEDVTLLDKELIIEGALTPDGGLIDEEFEIKALVEEFAELAGNVGIELPLPTREGALADEPIDWDEELEPEKPVLWVVTTLVEEKLEPVGPVLRVVITLVEEVLEPVRPVLWVGTALVEEELEPVGPVLWLVTTLVEEELEPVGPVLWLVTTLVEKELDPVGPVLWLVTTLVEKELKPVGPVLWLVTKLVEEDSEPEGLVFWPVTTLVEEDRPLLGRMDEEGANEASRSFGLASKYPGHVFTGYCQLCCSLSEWKMYRPDACVKDIAVQLAGIGARKYAWMAPVGSLHKEAVPLTKRLFCHESVPAVNCQVVEILPTLPEGAKSADITPGELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ55</accession>
    <name>A0A8H6CJ55_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">U3 small nucleolar RNA-associated protein 25</fullName>
            <shortName evidence="10">U3 snoRNA-associated protein 25</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_010728</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">DEAD-box RNA helicase-like protein required for pre-18S rRNA processing, specifically at sites A0, A1, and A2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
            <location evidence="2 10">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the UTP25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153214.1">
        <property type="nucleotide sequence ID" value="XM_037301580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ55"/>
    <dbReference type="GeneID" id="59339118"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002356">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010678">
        <property type="entry name" value="UTP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053939">
        <property type="entry name" value="UTP25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053940">
        <property type="entry name" value="UTP25_NTPase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933">
        <property type="entry name" value="ORF PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06862">
        <property type="entry name" value="Utp25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22916">
        <property type="entry name" value="UTP25_NTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7 10" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP25 NTP hydrolase-like" evidence="13">
        <location>
            <begin position="258"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="UTP25 C-terminal" evidence="12">
        <location>
            <begin position="527"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6224154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="724" mass="82283" checksum="D835F541BFD3AC9B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJR2</accession>
    <name>A0A8H6CJR2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mitochondrial import inner membrane translocase subunit Tim21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010013</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TIM21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153686.1">
        <property type="nucleotide sequence ID" value="XM_037300869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJR2"/>
    <dbReference type="GeneID" id="59338405"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.320">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit Tim21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013261">
        <property type="entry name" value="Tim21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038552">
        <property type="entry name" value="Tim21_IMS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032:SF6">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08294">
        <property type="entry name" value="TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="163" mass="17760" checksum="E91B2BF71BB5D8D6" modified="2022-01-19" version="1">MMECRIVFGARFGVAVAAKSQQQLLSSRIVGRRCLPFAIHRCYATSNTLAGTPKPSSRKQVTIGNDDGRVPWGQLSAGEKAARTTQQAFNFGVILAGAVGLAAVSYYLYIDVFSSDSKTRHFNRAVDRIRVDPRALNLLGSGQTIRAFGEPTASKWSRNRPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKG5</accession>
    <name>A0A8H6CKG5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA pseudouridine synthase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">tRNA pseudouridylate synthase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">tRNA-uridine isomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_010175</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr). Catalyzes pseudouridylation at position 44 in U2 snRNA. Also catalyzes pseudouridylation of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine in snRNA = pseudouridine in snRNA</text>
            <dbReference type="Rhea" id="RHEA:51124"/>
            <dbReference type="Rhea" id="RHEA-COMP:12891"/>
            <dbReference type="Rhea" id="RHEA-COMP:12892"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153847.1">
        <property type="nucleotide sequence ID" value="XM_037301030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKG5"/>
    <dbReference type="GeneID" id="59338567"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02568">
        <property type="entry name" value="PseudoU_synth_PUS1_PUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041708">
        <property type="entry name" value="PUS1/PUS2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF4">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="17">
        <location>
            <begin position="465"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6224980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="674" mass="76639" checksum="23FD300910408116" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKJ5</accession>
    <name>A0A8H6CKJ5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154136.1">
        <property type="nucleotide sequence ID" value="XM_037301319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKJ5"/>
    <dbReference type="GeneID" id="59338857"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="51" mass="5810" checksum="8D36C498DB2E251C" modified="2022-01-19" version="1">MPSRVHKDGRRDDEALREVLAVDAKNDGMRIYRITLVRTLPRLSDVTTNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKW5</accession>
    <name>A0A8H6CKW5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010432</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154102.1">
        <property type="nucleotide sequence ID" value="XM_037301285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKW5"/>
    <dbReference type="GeneID" id="59338823"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051614">
        <property type="entry name" value="UPF0045_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00106">
        <property type="entry name" value="MTH1187 family thiamine-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777:SF1">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="MTH1187/YkoF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-binding protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="119" mass="13153" checksum="6F39DE65CC1F3783" modified="2022-01-19" version="1">MMAPPKDLDLETLATPENCVADFCLIPIGTTVSVSQEVADVQRLMQKSHLSYSMHSAGTTVEGPWDDVMRLIGQAHFMLHQKGVVRIQTDIRVGSRTDKKQKFSEKVTAVNEILAADVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLE4</accession>
    <name>A0A8H6CLE4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154347.1">
        <property type="nucleotide sequence ID" value="XM_037300499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLE4"/>
    <dbReference type="GeneID" id="59338033"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000146">
        <property type="entry name" value="Clathrin-coated vesiclec protein (Bud7)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000149">
        <property type="entry name" value="Clathrin-coated vesiclec protein (Bud7)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="692" mass="77395" checksum="49B31455635E7BDE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLQ5</accession>
    <name>A0A8H6CLQ5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154547.1">
        <property type="nucleotide sequence ID" value="XM_037300699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLQ5"/>
    <dbReference type="GeneID" id="59338235"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="378" mass="43556" checksum="0680709ADBBC7022" modified="2022-01-19" version="1">MPKPKKSSGKPPAKPTAPNTSSTSPIPSPFKPAPAELSTFLDTLTSKSHLYIIHLDTHPPAFKRRIFTVPLLLNIFILIILVFRLQRALPTYFYLLLSVLGYDSPANIDKENTDQWRLLGISGERTLMFAGDFLLFRFVGMWPWDFFLGKGGEASPVGWRRWCRFRDVEVVVRRSRRWDVPLFATERSREEEDMRGEVEKKEGVKEEWLEEGRQGKLFRERVLPAVDKGWVKSKTSYQMLDQNWDLYFSGMIEAQALVDVGKNKIDDFRTSVLVHSEKSGWLIWEVWREQEEGAGDEGTRKLQLIKNHLTAMGKENLFFRWIEIVQSETSQTGPFTGERQKKAVQKIREEFEEQGVDFEGFWGSVGGVEGMPGMEITT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLT6</accession>
    <name>A0A8H6CLT6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009451</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154160.1">
        <property type="nucleotide sequence ID" value="XM_037300312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLT6"/>
    <dbReference type="GeneID" id="59337846"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="155" mass="17352" checksum="23516EF3DA276279" modified="2022-01-19" version="1">MATPMTEPVERKAYDSEITSACSSWDTALIRARSVTVSTPPSAIPATPKENTVVHRGVDNVESAIKLVARSMRSADIMISDTSSAQPSRHWIIDLSARKTGSLTLAFLLGHFSERRALYEASITQRFKFKHKRDDFRKLFAPEISEGRLIIVAWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLU7</accession>
    <name>A0A8H6CLU7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor CWC24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009596</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the CWC24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154305.1">
        <property type="nucleotide sequence ID" value="XM_037300457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLU7"/>
    <dbReference type="GeneID" id="59337991"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034247">
        <property type="term" value="P:snoRNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16539">
        <property type="entry name" value="RING-HC_RNF113A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000045">
        <property type="entry name" value="RING finger protein 113A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039971">
        <property type="entry name" value="CWC24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930:SF0">
        <property type="entry name" value="RING FINGER PROTEIN 113B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930">
        <property type="entry name" value="ZINC FINGER PROTEIN 183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6 7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="251"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="7">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="309"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="325"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6225596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="339" mass="37182" checksum="2DB872E34BB99A56" modified="2022-01-19" version="1">MDTTVLQERTPTDTQPLFKKRGAKNIRKRPATPPPAASDSESGYTSKEDEGRRVKRRKKTAIVTASSHNAPKKGTEDLSATKFAADRSAQISETNDATKTSNWFDESTPDAKSLLGTTRNGHVKPDREAEPTNDGTYKGAAGYQSFIQKNPNAPTRTVGPVKAPTNIRTITVTDFAPDVCKDYKQTGWCGFGDSCKFLHAREDYKQGWELDKDWEKVGDKGKKGGKAVKSLAEAEDSEEEDAALEGIPFACIICKKAYTNPIVTKCGHYFCEACALQRYRKNPSCAACGAGTGGVFNGAKNLKKILDRKRERARKRREKAKEAGEEVSEEDEEGGEEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CME0</accession>
    <name>A0A8H6CME0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008953</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154641.1">
        <property type="nucleotide sequence ID" value="XM_037299815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CME0"/>
    <dbReference type="GeneID" id="59337348"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11059">
        <property type="entry name" value="CYP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="44037" checksum="027500AABEDA45A3" modified="2022-01-19" version="1">MFTMLGHKPHSVQKRMVSNLYSKSYLQSSKDLQAISDCLFFDRFLPIMHEVDEHGGEMDVLDFMQAVGMDFTSAYLFGLSNGTDFMNDAAYRRHWLEEFKTFKVRLPQERAGGEIERWCLSMCEAAERFMNSEKVRDGPLETQPVVYGRLAHSLREAAQSDAPSLKPTMMTAASEMLDHLVAGHETSGIALTYLMHELSQRPDLQDHLRRELLTLSPPLLNPARDDGANANAGRCLPSPRSIDALPLLNNILQETLRLYAPAPAQQPRLTPFSPNGTTIDGYGKIPGGVRVSANPYCLHRNAAVFPAPEKWVPERWDVGGEEGEEMRRWFWAFGSGGRMCLGSHFALQEMKLVVAAVYTNWTTEIVHGGGMEQADTFISGPVGEKCVLRFKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMY7</accession>
    <name>A0A8H6CMY7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154619.1">
        <property type="nucleotide sequence ID" value="XM_037299793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMY7"/>
    <dbReference type="GeneID" id="59337325"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="685" mass="69013" checksum="273E51142BE2FE80" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP04</accession>
    <name>A0A8H6CP04_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ureidoglycolate hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008419</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(S)-ureidoglycolate = urea + glyoxylate</text>
            <dbReference type="Rhea" id="RHEA:11304"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57296"/>
            <dbReference type="EC" id="4.3.2.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155286.1">
        <property type="nucleotide sequence ID" value="XM_037299285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP04"/>
    <dbReference type="GeneID" id="59336815"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004848">
        <property type="term" value="F:ureidoglycolate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050385">
        <property type="term" value="F:ureidoglycolate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20298">
        <property type="entry name" value="cupin_UAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.480">
        <property type="entry name" value="Ureidoglycolate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047233">
        <property type="entry name" value="UAH_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007247">
        <property type="entry name" value="Ureidogly_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024060">
        <property type="entry name" value="Ureidoglycolate_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221">
        <property type="entry name" value="UREIDOGLYCOLATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221:SF1">
        <property type="entry name" value="UREIDOGLYCOLATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04115">
        <property type="entry name" value="Ureidogly_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="138"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="24868" checksum="F10028BC7B448808" modified="2022-01-19" version="1">MAPSSTITVDTVPLTPSAFAPFGTVLISPLSSATTTPPTPPPRGSISANQGTALKFPDIAPVTSIYHTSPSGALAKPSMSLFSCFSRALRTSNGRQIFDVKVLERHPFTTQTFIPLATSSPSSSVITSTTKSIIIVAPTLPTPPPPPPSPPNLTPYSPQLQSKQGGPPDLKNLKAFMAEPGMGVTYGVGTWHAPMVVVGGRVDFVVVQHVSGRGDEDCQEVDVQGVEVVVEGAERAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPG7</accession>
    <name>A0A8H6CPG7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HNH nuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155461.1">
        <property type="nucleotide sequence ID" value="XM_037299460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPG7"/>
    <dbReference type="GeneID" id="59336990"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13391">
        <property type="entry name" value="HNH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH nuclease" evidence="2">
        <location>
            <begin position="200"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="442" mass="50241" checksum="D5514E6F1979C308" modified="2022-01-19" version="1">MAHFQPLVKPETQLTSSRFDMDLDSSVLDSVDCTPKTKNLMMSLKRKASQLLDQGSPHTTSPFLKWRLAVVESTLRVRAVQKQALSEANTFFDDAGKPKTEFVALVKEDEKVLLSEKVFLLSERKVLEEDLNDIVTSREQLKDAYITELRISLEAASSSKERLPGLKAPRLDRKQFRDSVNEYLGTKTENPDTEDSRIWCNVLGYWLGSGSVKCAHIVPYSWNTKDTAHMFGSDEAPLTSRRNGLSLQKKIEEGFDNCWITIVPVDSVESSPTEWKVILLNTAERDKPFFEDMSKETDRGLWRWRDIDGRKLSFPNDNRPARRFLYMRYALAWLHADDKSWPGFQQKLPPGGVWASPNKPDGYLRKSILLEVGKKTGDRLPKDLVSAGIFEDPDTSSVVHDEVAGIRVAELVQGHLDGMRDSKVDEDSRERAGEEEDLMEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPV1</accession>
    <name>A0A8H6CPV1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008861</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155725.1">
        <property type="nucleotide sequence ID" value="XM_037299724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPV1"/>
    <dbReference type="GeneID" id="59337256"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051615">
        <property type="entry name" value="Transcr_Regulatory_Elem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313:SF81">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="136"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="87"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6227417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="396" mass="43533" checksum="32172CF9B0494F75" modified="2022-01-19" version="1">MSSNNFSTSAPNSRAPVLPQTLVSLADPPLLGGDEPLIFDELTGPYAMPPNDWYEWEDPMPTVTSVPGLQASPELWVPVDAVSIAPRQRHRAPAPTRQETEARHPELANKPEPKRRKAPPVKGVSGRVIEKKVEQACMRCRAAKQKCDGEKPHCRRCTRRNRRCSYVPTERQIEWAQKKAAGSANTPPESVEQPRTVAMTPPENYVLTAPGRRLLPRPEAPEAAMGGFTAGAEAQLQDPEYFDRALEDLNTMTNGYSQYPQQPCWTSGELDLWSTGALQYPPQPNLAFRGYLSQPSRPLPFLGEPSLTSEGSVGQSTGFSLYTRDPSMASDGPTAFSENSTHQNEPGMGFMNYDFPQTSPEGGQDLTGSNHFDGQAWTLAHFAGLDAMDLPGNRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR75</accession>
    <name>A0A8H6CR75_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156324.1">
        <property type="nucleotide sequence ID" value="XM_037298988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR75"/>
    <dbReference type="GeneID" id="59336517"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="555" mass="62710" checksum="A08377A89ECC3EE6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRX0</accession>
    <name>A0A8H6CRX0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">JmjC domain-containing histone demethylation protein 1</fullName>
            <ecNumber evidence="5">1.14.11.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">[Histone-H3]-lysine-36 demethylase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_008084</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 2-oxoglutarate + 2 O2 = L-lysyl(36)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:42032"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="1.14.11.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the JHDM1 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.27" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156288.1">
        <property type="nucleotide sequence ID" value="XM_037298952.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336481"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140680">
        <property type="term" value="F:histone H3K36me/H3K36me2 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15517">
        <property type="entry name" value="PHD_TCF19_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041070">
        <property type="entry name" value="JHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050690">
        <property type="entry name" value="JHDM1_Histone_Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23123">
        <property type="entry name" value="PHD/F-BOX CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17811">
        <property type="entry name" value="JHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="21">
        <location>
            <begin position="478"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="22">
        <location>
            <begin position="739"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="90"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="229"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="290"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="622"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1065"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1171"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1465"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="229"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="629"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1072"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1171"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1191"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1471"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1500"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1511"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1522"/>
            <end position="1532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAF6228354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1589" mass="176072" checksum="D9AF33115CB5CF34" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6U0</accession>
    <name>A0A8H6F6U0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147092.1">
        <property type="nucleotide sequence ID" value="XM_037297656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6U0"/>
    <dbReference type="GeneID" id="59335160"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="757"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="849" mass="95694" checksum="111FFD1E1C352979" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7E7</accession>
    <name>A0A8H6F7E7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147166.1">
        <property type="nucleotide sequence ID" value="XM_037297456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7E7"/>
    <dbReference type="GeneID" id="59334959"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="218" mass="24476" checksum="C5B1CC291FF920EE" modified="2022-01-19" version="1">MPDLPAKTILLALDREDDAKTKSSGLSWLSIIEVMGITPGLHGKHWRRYHHGHQFTNPNAIGHLSRSLSLSNVGLWNGNKDFSFSDPNNYLELRHKAPPAVVLSTDQDSEYSREAIQAKSGTAFDHVDLHSFQRTIYENADGAHWHISHGPGGLPDFDWIEEQNNFELETDSQTGKQMAASTLVRTSFPRLSLRSHPSTEYHQSSSSTDLTTRHTYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7I2</accession>
    <name>A0A8H6F7I2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="4 14">1.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_006695</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="14">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="14">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="14">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 14">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="4 14"/>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147028.1">
        <property type="nucleotide sequence ID" value="XM_037297592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7I2"/>
    <dbReference type="GeneID" id="59335096"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000006">
        <property type="entry name" value="3-isopropylmalate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004429">
        <property type="entry name" value="Isopropylmalate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00169">
        <property type="entry name" value="leuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979:SF1">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 14" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0520">NAD</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="15">
        <location>
            <begin position="5"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6217593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="367" mass="39636" checksum="164A65E8A7049730" modified="2022-01-19" version="1">MPPYNIVVFAGDYCGPEVTAEALKILRVVEKERPHIKFDFQEHLLGGASIDATGSPLTDEALAAAKKADAVLLGAIGGPKYGTGPVRPEQGLLKLRKELNTFGNLRPCNFASDSLTSISPLKEHICRGVNFTIIRELTGGIYFGERKEEENNDGYAWDTEPYSRAEIERVTRLAAHLAMATEPPQKVWSLDKANVLATSRLWRKVVSEVMKNEFPQLQLGHHLIDSAAMLMVKDPRALNGVIVTSNLFGDIISDEASVIPGSLGLLPSASLNGVPDGKSVVSGIYEPIHGSAPDIAGQEKVNPVAMILSTAMMLQYSFNQPEEAKAIEEAVRRTIDANVRTGDIGGKSTTKQVGDHVAQELSKILSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7I9</accession>
    <name>A0A8H6F7I9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147602.1">
        <property type="nucleotide sequence ID" value="XM_037297028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7I9"/>
    <dbReference type="GeneID" id="59334530"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15447" checksum="06FFD8F944C72D1C" modified="2022-01-19" version="1">MAARDAMLADATAAKSKEKPKPTTPASRQRTQPQQRARFQQPDVNQNKKWMGVNKTSADFKVLDYGAGTGFLSMAFAPHVSKVHAIDSARGMVDEFNKFAAKTATKRAYPNCKMKAVWGGLIDHEKPQDPKIANAQPSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8A2</accession>
    <name>A0A8H6F8A2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005782</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147868.1">
        <property type="nucleotide sequence ID" value="XM_037296687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8A2"/>
    <dbReference type="GeneID" id="59334187"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF95">
        <property type="entry name" value="NUCLEOLAR PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="93"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="209"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="335"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="335"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="405" mass="44547" checksum="43412699E99E1D5A" modified="2022-01-19" version="1">MGRHSTAIDTTTLTPPLSPSSKRKREEITAEEIEVDVTAPEPPSKKALRKAKKGKATPDASKAESKSIGVQAGSVLDEDASADISATSKRSEYGIWIGNLPWTATKADLRNFLTKDTGISENMITRLHLPAPRQGIVTNSKQRIKPQNKGFAYIDFSTEEALTEALALSERLLTGRRVLIKDSKSFEGRPDRTKEQETAIVNSGKPPSKRIFVGNLAFETTKQDLETQFTRCGEMLDVHIATFEDTGKCKGYAWVEFAELEAGEAAMRGWVNFEQKADSGDEDEEIAEALAGEADVKPKKKPKLRKWWVNKLHGRPLKLEFAEGKDVRYKKRYGKDGSASKDGVKVVDPEPESMAQPAFKYPTSSAKRPLRRLDARTVKPGAALAAAPRLTGGIVESQGKKTTFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8N4</accession>
    <name>A0A8H6F8N4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148787.1">
        <property type="nucleotide sequence ID" value="XM_037296089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8N4"/>
    <dbReference type="GeneID" id="59333583"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="76" mass="8021" checksum="C5513264F6C19461" modified="2022-01-19" version="1">MDGENNESDANAKEEAVGDEGAFNVLDDTDCRHANEANGFTTEPVNAVYFVVPSKSAEAPPVGKNYPNTTGISTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9I9</accession>
    <name>A0A8H6F9I9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003821</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149431.1">
        <property type="nucleotide sequence ID" value="XM_037294743.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332232"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055028">
        <property type="term" value="C:cortical microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043622">
        <property type="term" value="P:cortical microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31949">
        <property type="entry name" value="GASTRIC MUCIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31949:SF21">
        <property type="entry name" value="OS05G0316300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034047228" description="LysM domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="3">
        <location>
            <begin position="32"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="781"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1133"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6219996.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
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    <sequence length="1718" mass="171677" checksum="FD4FCAA451F54AF3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA10</accession>
    <name>A0A8H6FA10_9LECA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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            <taxon>Pezizomycotina</taxon>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
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    </dbReference>
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            <begin position="81"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6220700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="378" mass="42472" checksum="A52AC271EF9A626D" modified="2022-01-19" version="1">MHPSGTNPDGSMILSFSDFWGEQQQLFRLTDLCDRAGCHCQGAEMLCFNWGYVLFEQALYETYREDCENACWCQEEASTESDSESDDSSVSWSSSDTWSSADSTSRDVDWAAENAQLLRNYYASVVRTFDTSTGVAPNRLNNGIPCRAGQAAGWVFASGASGKCCPGYSYTALTASEAYVLYGLPIGSIVAGVRSIGMCLKAVSGLPPVYARLKFPAAHVYVDNEWITIAHIASETINIRECVPETIESLETLQYLGLHQDAALEAFESIEWAKRHVWNLPDGSEDDTVSEWDDVMRIMGVQDLWRNAILDPRFARPRKEHTARHWVIQTMEEKWKHLVELDQRMKLDPADIEAVKHRIGYRANLLRKYAVNGSDSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA90</accession>
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            <fullName evidence="4">HTH La-type RNA-binding domain-containing protein</fullName>
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        <name evidence="5" type="ORF">HO133_002651</name>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF132">
        <property type="entry name" value="LA-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
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    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LARP1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="4">
        <location>
            <begin position="694"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="27"/>
            <end position="661"/>
        </location>
    </feature>
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        <location>
            <begin position="886"/>
            <end position="906"/>
        </location>
    </feature>
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        <location>
            <begin position="922"/>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="104"/>
        </location>
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        </location>
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        </location>
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        <location>
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        </location>
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        <location>
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            <end position="472"/>
        </location>
    </feature>
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        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
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        </location>
    </feature>
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        <location>
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            <end position="601"/>
        </location>
    </feature>
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        <location>
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            <end position="632"/>
        </location>
    </feature>
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        <location>
            <begin position="641"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="922"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="947"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1162" mass="125984" checksum="82B68164B4A9D0FF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAA5</accession>
    <name>A0A8H6FAA5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="6">2.3.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_002846</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + acetyl-CoA = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:17017"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="2.3.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="6">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.12" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149849.1">
        <property type="nucleotide sequence ID" value="XM_037293771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAA5"/>
    <dbReference type="GeneID" id="59331258"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000003">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006257">
        <property type="entry name" value="LAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01349">
        <property type="entry name" value="PDHac_trf_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 6" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="8">
        <location>
            <begin position="55"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="9">
        <location>
            <begin position="213"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="145"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="500" mass="52604" checksum="55D463797E970B11" modified="2022-01-19" version="1">MAASVLLRAPVRACLRAPSSSLGASALTRSQNASRYIPALSALARLYASKSFPPHTVVSMPALSPTMTAGNIGTWQKKAGDAIAPGDVLVEIETDKAQMDFEFQEDGVLAKILKDSGEKDVAVGNPIAVMIEEGGDVSAFESFSLDDAGGEKAPPKDAPKEEAAESSEPSNSGSKTSPDTLSKDEPKSAPPPEPQESESSGGRLETSLDREPKASPSAKALALERGVPIKQIKGTGPGGRITKQDIEKFKPESSAAPTAAAAGAPTYEDIPASSMRKTIANRLTQSFNQNPHYFVSGTLSVTKLLKLRQALNASADGKYKLSVNDFLIKASAVACKKIPAVNSSWREVGGQVVIRQHNTVDVSVAVATPIGLMTPIVRGVDSLGLEGISAQVKDLGKRARDGKLKPEEYQGGTFTISNMGMNAAVERFTAVINPPQAGILAVGTTKKVAIPSTSEDGGVEWDDQIVVTGSFDHKVVDGAVGGEWIREFKKVVENPLEMML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBI6</accession>
    <name>A0A8H6FBI6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase regulation protein NCA2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001702</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151169.1">
        <property type="nucleotide sequence ID" value="XM_037292630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBI6"/>
    <dbReference type="GeneID" id="59330116"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013946">
        <property type="entry name" value="NCA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234:SF1">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08637">
        <property type="entry name" value="NCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="14"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="76239" checksum="3DFF1F465D5408BF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC02</accession>
    <name>A0A8H6FC02_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151679.1">
        <property type="nucleotide sequence ID" value="XM_037292260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC02"/>
    <dbReference type="GeneID" id="59329746"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="145" mass="16025" checksum="7FDA057551AD03D1" modified="2022-01-19" version="1">MDPLSLIASIIAIVGAGGSVGKGLKKLASIRHAERDLVLLQDDVSDLQLLVGNVSEIILEFEKTTKRPSYQRIGRALERTKSTILDLEKLIVYNLTTIADSHGKLRIDPWAWITHEAAPRTAIAARDYVSENRTIRQIDNNTIGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCK5</accession>
    <name>A0A8H6FCK5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000955</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152250.1">
        <property type="nucleotide sequence ID" value="XM_037291888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCK5"/>
    <dbReference type="GeneID" id="59329373"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000557">
        <property type="entry name" value="WD repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051859">
        <property type="entry name" value="DCAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19847">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19847:SF7">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="349"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="384"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="431"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="690" mass="76675" checksum="21606CB7FA69B508" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCU4</accession>
    <name>A0A8H6FCU4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-rRNA-processing protein RIX1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000483</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RIX1/PELP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152857.1">
        <property type="nucleotide sequence ID" value="XM_037291422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCU4"/>
    <dbReference type="GeneID" id="59328902"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012583">
        <property type="entry name" value="RIX1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105:SF1">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08167">
        <property type="entry name" value="RIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein RIX1 N-terminal" evidence="6">
        <location>
            <begin position="9"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="731"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="732"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="768"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="809"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="822" mass="89354" checksum="7F3D95C39C467EDA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCY7</accession>
    <name>A0A8H6FCY7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Elongation factor 1-gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000334</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152708.1">
        <property type="nucleotide sequence ID" value="XM_037291273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCY7"/>
    <dbReference type="GeneID" id="59328753"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03181">
        <property type="entry name" value="GST_C_EF1Bgamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03044">
        <property type="entry name" value="GST_N_EF1Bgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000006">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000142">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1010:FF:000001">
        <property type="entry name" value="Elongation factor 1-gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1010">
        <property type="entry name" value="Translation elongation factor EF1B, gamma chain, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036433">
        <property type="entry name" value="EF1B_G_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89942">
        <property type="entry name" value="eEF1-gamma domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50040">
        <property type="entry name" value="EF1G_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="8">
        <location>
            <begin position="88"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="6">
        <location>
            <begin position="258"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="217"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="47883" checksum="6A3D599FAD89C9BE" modified="2022-01-19" version="1">MSFGKLYTREGNARSTAIRAVAKANKLDLEIVDTDVSKGPLSTEYLKLNKLGRIPSFVGADGYELTECIAIAIYVTSQNEKTSLLGKTKQDYASIVRWMSFANAEVLPALGGWFRPLIGRDPYNKKNVDEASKNALKAVSVLNEHLLVHTYLVGERITLADLFTAGIISRGFEFFFDKHWREENPNVTRWYETVCNQPIYSDIAGKLDFIDEAIKNQPPKTEKQEKPKKEQAPKAQPKPKAKEIEEEEEEKDKPAPKPKHPLEALPKPTLALDDWKRKYSNEETRAVALPWFWENFNAEDYSLWKCDYKYNDELTQTFMTANLIGGFFTRLEGSRKYIFGCASVYGMKNDSIVQGAFVIRGQEALPAFDVAPDYESYEFIKLDPSKAEDKEFVNDQWSWDKPIEVQGKTYEWADGKVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE24</accession>
    <name>A0A8H6FE24_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AFG1-like ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010139</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153812.1">
        <property type="nucleotide sequence ID" value="XM_037300995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE24"/>
    <dbReference type="GeneID" id="59338531"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001493">
        <property type="entry name" value="Mitochondrial ATPase (Afg1), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF6">
        <property type="entry name" value="AFG1-LIKE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="60594" checksum="AF02E6201AA68456" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FES4</accession>
    <name>A0A8H6FES4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010366</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NOSIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154036.1">
        <property type="nucleotide sequence ID" value="XM_037301219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FES4"/>
    <dbReference type="GeneID" id="59338757"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000673">
        <property type="entry name" value="RING finger domain protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016818">
        <property type="entry name" value="NOSIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13063">
        <property type="entry name" value="ENOS INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13063:SF10">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023577">
        <property type="entry name" value="ENOS_interacting"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="244"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="119"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR023577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="359" mass="39124" checksum="CB6721310A923DD4" modified="2022-01-19" version="1">MAHSKRNTSLAFFTSHERSLLKSTWGSQATRLSRDSFLPFASCQLCLRIARDPVACASNGDIFCRECAVSNLLAQGKEIKRLKKEGERRQREVEEEELDRGAEEEERAIAQFEKTMMGLEGGGKKEGKSNASEEMERSEGRGVKRKFELDEDEMLKNAKEERAKARKALDEEKSAKPTLPSFWVPSLTPSTNHSQASKAAKLSPLCPASSTENKHSLSLKSIITINFKTIPNASSPTEPPTCICPACEKNLTNTIKAILTIPCGHVLCKPCAAKFMSPDEGPPDPHASPTKQEAKNRVVCYVCETDLSGKKITAKDGEDGARAEKGAVKSGTVELRSEGTGFAGGGDNMVQRKGVAFQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF33</accession>
    <name>A0A8H6FF33_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154223.1">
        <property type="nucleotide sequence ID" value="XM_037300375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF33"/>
    <dbReference type="GeneID" id="59337909"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="60080" checksum="780EF93665955408" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFL3</accession>
    <name>A0A8H6FFL3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154879.1">
        <property type="nucleotide sequence ID" value="XM_037300053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFL3"/>
    <dbReference type="GeneID" id="59337587"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="249" mass="28700" checksum="B161AA5E5660909E" modified="2022-01-19" version="1">MAPTLHRKLFDKTPQRTDDERLPPPTQSSKSSETLVTEGSDTDIYKKPSSAEEKGRNKLDIKTSCVQICPHETLTFERMKRIVHLPYFRYSGDKVDGFTNGFGPYHVSSVVGNHLCKPHPNRFSSLKANSFYKYQRGYEGSYDGLLLCVHWTMSFDDHMDVASSVSDFQRFLDILDIRLCQHTKMSDSRIAAKLYKLCDSSRMARDPVEAYEEGYRGCITERCKECHTTFETYKEGEFRIREAVVGSMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFS6</accession>
    <name>A0A8H6FFS6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009114</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIL4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154801.1">
        <property type="nucleotide sequence ID" value="XM_037299975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFS6"/>
    <dbReference type="GeneID" id="59337509"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12235">
        <property type="entry name" value="RRM_PPIL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000287">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035542">
        <property type="entry name" value="CRIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="75"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="165"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="212"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="238"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6226248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="274" mass="32360" checksum="716E16ED7B4DBAC7" modified="2022-01-19" version="1">MSEFDEKLQVQETGSGHDVKNDEKIEETEIFGEVDEEAEEEAREKLRREREARAQALTLEIVGDLPYAEVKPPENVLFVCKLNPVTEDEDLDTIFSRFGPIVSCEVIRDKRTKDSLQYAFIEFENQKDCEQAYFKMQGVLIDDHRIHVDFSQSVSRLSDKWRTATNSKRAQHGGGFGGIAGLEKKRQFRAIDPKSDEDRKRSNGYGMVIDKNAIRHERDKEPDRRDRPRKHSRSRSPRNHDRYRERVPSGDVRRRRDRSRDYQRDDYEDRSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFU7</accession>
    <name>A0A8H6FFU7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase family 62 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008413</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155280.1">
        <property type="nucleotide sequence ID" value="XM_037299279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFU7"/>
    <dbReference type="GeneID" id="59336809"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF4">
        <property type="entry name" value="N-GLYCOSYL-TRANSFERASE (AFU_ORTHOLOGUE AFUA_4G06870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034921555" description="Glycosyltransferase family 62 protein" evidence="3">
        <location>
            <begin position="32"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="50549" checksum="A311B6944A2CA035" modified="2022-01-19" version="1">MFPLFRKNSSSNLSTKLCLLLLLSECTAAWALPFFSTNAPSSLDEQDRWLSTPSEVIHRSFRPTPPAPNSEDRAQKDRVLILTPLKDASFHLPHHFALLSNLTYPHHLIDLGFIIGDTVDDTRAVLDAELDKIESGKQDKAFNSCTTVLQNLGDVASQEVAARHGFAAQVDRRKKIAIVRNTLLQKTLKSTHDWVYWRDVDIAESPDGMLEDLIAHDKDVLVPRPRLPDDYNSWQETPQSEAFKERLDTEVIVFEGYSQFKTRRRHLAKLGDWRNNAALEIPLDGVGGVSLLIRAEVHRKGILPKFLARSSIPPFTFFVTDQTGIDFPIKPVDHQIETEGLARMAKIAGYGVYGLPNYVIWHFDTSEKEGNLHETPAWLWPLLGIVAVAVIVLGLRHRKWSLYQMPELRQLGFARSAKVDQKGLVDVTPLSLEANKGRMVEAPTMLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGE5</accession>
    <name>A0A8H6FGE5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155638.1">
        <property type="nucleotide sequence ID" value="XM_037299637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGE5"/>
    <dbReference type="GeneID" id="59337168"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="233" mass="24406" checksum="DA55323FA8AE37C1" modified="2022-01-19" version="1">MSNNENSPPPTPTATTDRRASFSPGQKLSELFGRSPPNGNGTPAYPGPIATAAANAQAQQRRRTSIASLGLAGSPTQTSAFTNMRNRQSSIGSGSSPSTSINESAVEEGDAEPNTSPTSPFARRMSFGAKALRDVRTGGTGNANGRASTNASSAPSVKGRGEGFNWPEQIKNRAERSASINSQAPPAPATGPSNHQKAASVSAPEQPIKEMPKSPRKPDHFQERILKGDFYMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGI9</accession>
    <name>A0A8H6FGI9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155366.1">
        <property type="nucleotide sequence ID" value="XM_037299365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGI9"/>
    <dbReference type="GeneID" id="59336895"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="617"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="758"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="921"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="591"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="957"/>
            <end position="966"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="966" mass="104598" checksum="BAD79C931E241198" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHA4</accession>
    <name>A0A8H6FHA4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007839</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156045.1">
        <property type="nucleotide sequence ID" value="XM_037298709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHA4"/>
    <dbReference type="GeneID" id="59336236"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000033">
        <property type="entry name" value="Anaphase-promoting complex subunit ApcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044554">
        <property type="entry name" value="ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014786">
        <property type="entry name" value="ANAPC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057975">
        <property type="entry name" value="TPR_ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08672">
        <property type="entry name" value="ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25773">
        <property type="entry name" value="TPR_ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01013">
        <property type="entry name" value="APC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="8">
        <location>
            <begin position="527"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="855" mass="95754" checksum="189E5EC35E41B4E4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI57</accession>
    <name>A0A8H6FI57_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156606.1">
        <property type="nucleotide sequence ID" value="XM_037297971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI57"/>
    <dbReference type="GeneID" id="59335476"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034447487" evidence="1">
        <location>
            <begin position="21"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="284" mass="31203" checksum="5921B177242D1FD9" modified="2022-01-19" version="1">MLFLQKTLALAAILISFCAPTPLIRIQNFSKQNICYMVEYSNGTGTFPNETICGIDRGFWLNSGQTKDFKATGFDGEHFNGAVTAWLKNNTFRGARNEINLLNQSMAWYDVSYQYGISDGTCGPANSSDLSGERDSLGKANSAWKTLSYPIKNQLLKSPQYLRQGKNGSLVYINMGVEAFPYGAPDVVMFFQATAGFKAYMGSGSATDVTWPPNSVQAELVPLADKQTLSAPTDHIIWEDASTYARRRWEEGFGIRQEGHESAVLCGALLTAGRLGWITVVQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FID4</accession>
    <name>A0A8H6FID4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156741.1">
        <property type="nucleotide sequence ID" value="XM_037298106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FID4"/>
    <dbReference type="GeneID" id="59335613"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050972">
        <property type="entry name" value="SDr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34403">
        <property type="entry name" value="TOL-PAL SYSTEM PROTEIN TOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34403:SF8">
        <property type="entry name" value="TOL-PAL SYSTEM PROTEIN TOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="600"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="633"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="269"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="306"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="727"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="68"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="746" mass="82320" checksum="B9BA2DC0B6892599" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIF9</accession>
    <name>A0A8H6FIF9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007250</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156777.1">
        <property type="nucleotide sequence ID" value="XM_037298142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIF9"/>
    <dbReference type="GeneID" id="59335649"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000099">
        <property type="entry name" value="Ribosomal protein S12, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_uS12_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034104091" description="Ribosomal protein S12" evidence="4">
        <location>
            <begin position="26"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19144" checksum="A28D74C261C1EA1E" modified="2022-01-19" version="1">MPPSTMPTLLRLPFLLPSLFRPTLPSTFKKFNTITNRTLSTTTSLSATLMQVLKKPRQPQRARHPVSPALVNRPHMKGVCLRVVNNFKPKKPNSAERKVARVRLSSGRAVTCYIPGEGHNVQQHSVVLVRGGRSQDCPGVRYHLVRGAMDLGGVGNRITARSKYGTKKPKSAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIT3</accession>
    <name>A0A8H6FIT3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007445</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156971.1">
        <property type="nucleotide sequence ID" value="XM_037298336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIT3"/>
    <dbReference type="GeneID" id="59335844"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000002">
        <property type="entry name" value="External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000005">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 64 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF50">
        <property type="entry name" value="NADH DEHYDROGENASE (UBIQUINONE)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="537"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6229329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="690" mass="78129" checksum="CC0B379D715C1EE1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ43</accession>
    <name>A0A8H6FJ43_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007560</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157086.1">
        <property type="nucleotide sequence ID" value="XM_037298451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ43"/>
    <dbReference type="GeneID" id="59335959"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051104">
        <property type="entry name" value="FAD_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720:SF3">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01460)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="12"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="310"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6229444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="446" mass="48394" checksum="22BB655DD6443365" modified="2022-01-19" version="1">MSSTTTSLKPFSVAIIGGGIGGLCTAIALLKYPHIDVQVYEAAPTFGEIGAGVGIAPNAQQALELIAPEARAAYDKHATGNMWPKHAKTMANYVGEGEHEGELIHAQLNASGQQSVHRAHFLNELVKTVPSQRAHFNKRVDFLEDKRSAPVVVHFKDGTTAIADAVIGADGVHSAVRAHLLGKEAAKPVFAGSVAYRALVPMGKAVEKLGEEFAGNAFFLCGRGRVPIFLSISSFSVFGEAVAHESKGKASFSYPIDGGSLLNVVIFDFEQETWEHEKWVLPADPEALRRMFNTWGASSRALVELLLATHEPSVWAMWDHGPASTYTKGRIAIMGDAAHATTPYQGQGAGQAIEDALVLATLLAEVSDPNMIQSAFLVYDQVRRVRTQRVVSTSRDSGQLVGMKAEGVGSDLEKMRELLSFRMHWIWNRDILAQNREALQLFKEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK23</accession>
    <name>A0A8H6FK23_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157198.1">
        <property type="nucleotide sequence ID" value="XM_037295197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK23"/>
    <dbReference type="GeneID" id="59332687"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050491">
        <property type="entry name" value="AmpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021860">
        <property type="entry name" value="Peptidase_S12_Pab87-rel_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46825:SF14">
        <property type="entry name" value="BETA-LACTAMASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46825">
        <property type="entry name" value="D-ALANYL-D-ALANINE-CARBOXYPEPTIDASE/ENDOPEPTIDASE AMPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11954">
        <property type="entry name" value="DUF3471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="2">
        <location>
            <begin position="30"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S12 Pab87-related C-terminal" evidence="3">
        <location>
            <begin position="431"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6229941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="540" mass="58932" checksum="373647C8ACA5F71C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FLL3</accession>
    <name>A0A8H6FLL3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7924 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157841.1">
        <property type="nucleotide sequence ID" value="XM_037290967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FLL3"/>
    <dbReference type="GeneID" id="59328446"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057684">
        <property type="entry name" value="DUF7924"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25545">
        <property type="entry name" value="DUF7924"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7924" evidence="2">
        <location>
            <begin position="179"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7924" evidence="2">
        <location>
            <begin position="388"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="473" mass="51925" checksum="7AA38CFD43FF54F7" modified="2022-01-19" version="1">MTTLEEVDICSWASGVATSLDASSDSETQATPTKITHKRKRIDDYYDSSRQHLRRPGPSSHIALAERSPSTMNTPSSPQTRDAQHTQPVTPQTNTTKSSYNLRSRPPTSTSSSSDAKQTVNEATIRTNAAGQGMYFENEAAFQKYPDFAASIENMVSGSRDSIMRDESIKTFKDYRAANTTKNEKTLFGNLVPLIVKPARSVKTAKRTIEDELKFQVKTFESDGLDHLEDQLFVKNILPLLNPADATQQGLTTAKPDYVYGLKYPQFPDLRKPLLSANTEALIGIAPGLRHAFCSADFKGCQHSIEAAENQSQRSGATMVAARRALNRKAKGQLEPAMPKDRAAATKAVATDATAIDDAVTAAHTTINTSALGPQPNEISQEDLGADTSSYAFTISWSTHVAKIHVHWCEVPTKGIVIYHMNVVGCYLMTRDKDLTDFRSHIHNILDWGVGPKRKRELNELESEVAKHESGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYB6</accession>
    <name>A0A8H6KYB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongin-C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007074</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Controls sulfur metabolite repression, probably by mediating the inactivation or degradation of the metR transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156604.1">
        <property type="nucleotide sequence ID" value="XM_037297969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYB6"/>
    <dbReference type="GeneID" id="59335474"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18321">
        <property type="entry name" value="BTB_POZ_EloC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000035">
        <property type="entry name" value="Elongin C transcription elongation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039948">
        <property type="entry name" value="ELC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20648">
        <property type="entry name" value="ELONGIN-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="6">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="101" mass="11117" checksum="547EB469C2A0BDC5" modified="2022-01-19" version="1">MAASSSPYVTLISNDGFEFIVSRDAACVAGTVKKMLDPHSSFAESISGRCTLGTINGVVLEKVCEYLYYNLKHKDEKDVPDMEIPPELCLELLMAADYLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYX1</accession>
    <name>A0A8H6KYX1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Epoxide hydrolase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007540</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157066.1">
        <property type="nucleotide sequence ID" value="XM_037298431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYX1"/>
    <dbReference type="GeneID" id="59335939"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF35">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6229424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="406" mass="45503" checksum="151C07A7555F6EE5" modified="2022-01-19" version="1">MASATPFTVAVPNDRLERLRVKLELTEFPDELDDAGWDYGAPLADVKRLTAYWKDGFDWKHQEADINKLPQFQTQIKVDGYEALRIHFVHQKSESSKAIPLLFVHGWPGSFLEVVKLLPLLAKQDGGKLPAFHVVAPSLPNFGFSDGVKKRGFALKQYAETCHKLMLQLGYDQYVTQGGDWGSHITRVIGLQYPQHCKASHINYVRPAHAPTFTSSPALALQHAVTPYSDYEKAGLARTQWFEKEGCGYNEIQRTKPQTLGYGLADSPVALLAWIYEKLHDWTDHYPWTDDEILTWVSIYWFSTAGPAASTRIYYEVGHATGSDRSAIGEYIPHVKLGLAYYPKDLDIPPKSWGRILGPVVFECQHPDGGHFAAHERPEAMVHDLNEMFGKGGGAYGVIKGEAGYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZL8</accession>
    <name>A0A8H6KZL8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157326.1">
        <property type="nucleotide sequence ID" value="XM_037295325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZL8"/>
    <dbReference type="GeneID" id="59332816"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="238" mass="26425" checksum="7F686195755839F5" modified="2022-01-19" version="1">MELQLARVHAENPISIQPAESKHLTPMARIHLAAFRNDDCVKFLCSDETHWIIVNTMLGERLENRPYAISVAMNERLGRVVGWLCCSVVGYQGSSDWDGLAQLAWTVAAAFKAAEAQRKLSRTSGESEHNSRQAQRKMLREAIVSRTKVAQSLAMGDDVYLVINNVATDPENDLGGVTPKLICSVTDSADKDGLSVFAQVPLFAVADFEWAGFQQIAGFEPQLDIHQLKFMVRRPKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5N8</accession>
    <name>A0A8H6C5N8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146982.1">
        <property type="nucleotide sequence ID" value="XM_037297781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5N8"/>
    <dbReference type="GeneID" id="59335285"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="286" mass="32338" checksum="DA898AC48E4ACBFC" modified="2022-01-19" version="1">MVRMSTLVRADKSRDSTFVNVTSDSNFFLSQPAIWDYGMSAGEPQFPTIAGKKATIIVYEPVDDADGMLSPIHKACLDILQRLCQIRQAQNEASASEEPKTLDAFCDALRQRRWRNFTEPDKSISEDYYYAKSGGIEWLRNYYGARTFWADQWDTEQCTEARDRLLAEFLLLSAVMRRPFAPDSLPPLRVPQVTIDTYSTNSVQRSSRRSSWLAVSGGVDAIIGNDTGCDDWEQSIEALVQKANMMSADEGGVEEGNKQLASAPLGLKNRWRIWKILQGIEFRQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5P0</accession>
    <name>A0A8H6C5P0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_006919</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WHI5/NRM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000029">
        <property type="protein sequence ID" value="KAF6217403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146838.1">
        <property type="nucleotide sequence ID" value="XM_037297815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5P0"/>
    <dbReference type="GeneID" id="59335319"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013734">
        <property type="entry name" value="TF_Nrm1/Whi5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08528">
        <property type="entry name" value="Whi5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="252"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6217403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="304" mass="33367" checksum="370F7EFE0E2924AD" modified="2022-01-19" version="1">MAQSTPERRALVDLPVNTFGTPSSMTTVGKVSIGHKRQIQEVEEPEFAQPTSRVRVSPARPQTTLIDDVSSGQTQPALSGAMSVYPSAPHIIQVDNVTEEPAEEMGEGDSQNSYKDSAMSSVIDFDPDDTMISQQTAATEVTQPLRSRISQHAETLRLRLRLAHFKVQTNQINLPLSQLRISRQEALPSGESTTKRDEQPSLPKLLPAPVLLPTAFSARMIAHPQDLSSPPCSPDHGAQKESPEVFRTPALPRQRVQNAQRQLSSPPGSDERISKRRYEDEDNLTSSAVRGKAAIGLLGLRQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6D0</accession>
    <name>A0A8H6C6D0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006715</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147048.1">
        <property type="nucleotide sequence ID" value="XM_037297612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6D0"/>
    <dbReference type="GeneID" id="59335116"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="161" mass="18954" checksum="F3DB85723CC8C7BE" modified="2022-01-19" version="1">MPSHEMPWPQWTREENIICVWFSALGLAIPDIARIIEHKLGRPTRHPRGYFRQMDRINQEEAERGRPRLCGRDMLDWDRAAVDNFLILLTDDTDLLDHLLWFGEEHLGLLRTHHGGQYLGRGIPLNEAVDVEDMQYQWARLLSRHQRFYGNPARYGGGHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6X7</accession>
    <name>A0A8H6C6X7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006417</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147440.1">
        <property type="nucleotide sequence ID" value="XM_037297315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6X7"/>
    <dbReference type="GeneID" id="59334818"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033990240" evidence="2">
        <location>
            <begin position="18"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="369" mass="38441" checksum="87BB42FF33DE5D39" modified="2022-01-19" version="1">MHTSVLISTLLGAGALAFPQHKHAHLHEKKDVVFYSQWQEGPHTLVQEFINENKVVGTAPPLAPTSAAAVQVKADYAFPQGGHYSWAGEAGQLHERPSFPMTSAAAVPEAPSPVIASPVVSSTPPAPIVIQANPVDSPPASSSSSAPKSSGSTSGACNPTDGDAWYSPPCTGSSGASALDKMSNLRKTWNDTLTDYAWSPGLAQNAHNTAWLSTTWTAGVDSNGNPTETPDGEGRGWVMGHHLFNGSDGQCIAEGNNTESVGDLTPIEAMFLMWICERPNAAIQDMCTAINSTGGDADCWCDLLTDPSCPCGHAMIIIDPGTNSLGCYFMDAVAAADKADNAVNDIEKAKELGSPGNGAPGMLTCDFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C717</accession>
    <name>A0A8H6C717_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Catechol dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_006477</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147500.1">
        <property type="nucleotide sequence ID" value="XM_037297375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C717"/>
    <dbReference type="GeneID" id="59334878"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018576">
        <property type="term" value="F:catechol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009712">
        <property type="term" value="P:catechol-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03461">
        <property type="entry name" value="1_2-HQD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039390">
        <property type="entry name" value="1_2-HQD/HQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007535">
        <property type="entry name" value="Catechol_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050770">
        <property type="entry name" value="Intradiol_RC_Dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711">
        <property type="entry name" value="DIOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711:SF7">
        <property type="entry name" value="INTRADIOL RING-CLEAVAGE DIOXYGENASES DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04444">
        <property type="entry name" value="Dioxygenase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catechol dioxygenase N-terminal" evidence="8">
        <location>
            <begin position="32"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Intradiol ring-cleavage dioxygenases" evidence="7">
        <location>
            <begin position="112"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="36854" checksum="51B61CB721D0C8AE" modified="2022-01-19" version="1">MDPSKAKVPPMKDLTIDNITENTIRINSQSSDPRLTYVLERLVTHLHDFARETRLSTKEWMAALEFLTAVGQISSDVRQEFILLSDILGLSLLVDSIDHPKPARSTEGSVLGPFHSHEAEDMAHGDAMSHDAQGEPCLVLCTIKDSHGKPVPDVKIDIWETDSSGHYDVQYADREGPDGRCVMKSDQDGNFWFKAIKPVSYPIPHDGPVGKLLEKLQRHPFRPAHMHFMFERPGYDHLITALYTRGDPYETSDAVFGVKESLLVEFHPADKMIAKEYDVKIGSAVLEHDFVLVSDIEASDLRDERSKKALADLGRKVKLVHGLPVPDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C775</accession>
    <name>A0A8H6C775_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147575.1">
        <property type="nucleotide sequence ID" value="XM_037297001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C775"/>
    <dbReference type="GeneID" id="59334503"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034261434" evidence="1">
        <location>
            <begin position="21"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="194" mass="20975" checksum="FF90BF80C915E322" modified="2022-01-19" version="1">MRAIVLSLAYLFYTARTTFAVAFPKQSSGLWTNAPEDDTLPATSGNTTLRLEPNPSALLNVSALTITCLTEPPPRQPITLAAYYEAVQKILIRDDAFFPRRFELGPGGKNLRWQGGQCVIAFFNNQPLKTDAIPIILVAHVAALIANECITEAKGYLGGVAQIGPSRGTVGVFHTVPSGSEADERYEALIRAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7L5</accession>
    <name>A0A8H6C7L5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147693.1">
        <property type="nucleotide sequence ID" value="XM_037297119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7L5"/>
    <dbReference type="GeneID" id="59334621"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047168">
        <property type="entry name" value="LEC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024554">
        <property type="entry name" value="LEC1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024555">
        <property type="entry name" value="PX-associated"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185:SF2">
        <property type="entry name" value="FUNGAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12825">
        <property type="entry name" value="DUF3818"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12828">
        <property type="entry name" value="PXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX-associated" evidence="3">
        <location>
            <begin position="3"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="163"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="358"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="649" mass="71563" checksum="ED3D2013F8320BF2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7S2</accession>
    <name>A0A8H6C7S2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Starter acyltransferase (SAT) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147621.1">
        <property type="nucleotide sequence ID" value="XM_037297047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7S2"/>
    <dbReference type="GeneID" id="59334549"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Starter acyltransferase (SAT)" evidence="1">
        <location>
            <begin position="67"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="344" mass="37422" checksum="D388BA4F831BA785" modified="2022-01-19" version="1">MTGNKLQIKPDNDSRESTVFLFGPHVATFTKQSMETLIRPISQGPFRDWILDTISGLPTYLNGLNSIRPEVARAVPGSSQLADLDSWLRHGATDITSESTLPSIIVGPLVVLIQLTQYWHYLELLHQGQPPADLQASVVAASKSDSKFETLGFCFGLLGALSVASASNRQDFQKYGATAVRLAMLIGALVDAREVWDKTSGKGGSISFAIAWLDSKQASEVRRIIDARSPDAYIAVLYDEARATVTTSESTVPLLVKQLRAAGAIVAEIGIKGRIHSPDADRKIDTDALVQFCSSMPDLQYAVTANLALPTYDNRGNGDLVSSKHQESMTEMVLRSILVDQCNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C877</accession>
    <name>A0A8H6C877_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1688-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148208.1">
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    </dbReference>
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    <dbReference type="GeneID" id="59333722"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012469">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31687">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31687:SF3">
        <property type="entry name" value="PROTEIN URG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07958">
        <property type="entry name" value="DUF1688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="54631" checksum="F7B02EE9733A3204" modified="2022-01-19" version="1">MRIFNRKSAKIPSNGEIMDSGFNSSSLKSPPPTQASHKNSPFVSPSALDVTLPAPPDPTLDPAAYLRSIWAVRARCQLIYQRAKRNQLAHFDVDGSKFGETANYLVSIIKRDYAPDYSQIPSHGRWQHFEAGGRPRVEQLIATWPKTVDAQEKTRRLIDLFLVSVLLDAGAGTLWSYKSKESGKVYKRSEGLAIASLEMFKAGYFSSDTQEPCQVDSLGLKRLSVQQLAKGLQVTEYNPIAGLEGRAGLLSRLGDALKNSALFGAEGRPGHMLDYLLSHPTTQAASVPVITVPTLWTVLINGLSNVWPSSRTQIDGVSLGDAWPCSSMPQSPPGLPWETIVPFHKLTQWLCYSLMVPMRRVMGIHFAGTELLTGLPEYRNGGLLVDTGLLTLKEKDANRGLAQYRENSMVKGQPNMEVVPLFATHDDVIVEWRAVTVGFLDDLLVEVNGILGLSGSDKLSLAQMLEAGSWKGGRELAEVSRPNTKEPPIMIMSDGTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C883</accession>
    <name>A0A8H6C883_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147993.1">
        <property type="nucleotide sequence ID" value="XM_037296812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C883"/>
    <dbReference type="GeneID" id="59334313"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="390" mass="43795" checksum="470427CE1F50DA5B" modified="2022-01-19" version="1">MDTLTLPLTRYHKTNQNDDNWLNTRLVSSLRQQTQWKHSPAFGHQARNNSTASLWPERDDALSRESLLAEQVSLNRRLRQREDGAAKESIDTKRCRHSELSGKEPERRRFLPSRYRRRLKSASPHRVLAGLSGPQSEALSPCGRVKQKNVLKVMRGLRVRNVSDDGHRWSLEPVLRSEDHPVFVQPVKEFVMKRWRVFRSGRRSRSSIFALSEGTGKRLDYQRAASSISRDSRQLSRRTQITTMIQGSGISLGQQAPDNEAPSRASAGREQILTSAEASQDSTNHAQTTTRSEPLIAIECSDEDLPMESTDYRPSTELGPRPSLRFSESGQTRNRTSTLGTTIYNSPLIAEASPNSKKLESDGRTSSGTSSSMRDPNEFLHGGEIASASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8T8</accession>
    <name>A0A8H6C8T8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148241.1">
        <property type="nucleotide sequence ID" value="XM_037296259.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333755"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42064">
        <property type="entry name" value="YALI0F28677P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42064:SF1">
        <property type="entry name" value="YALI0F28677P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="576"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1209"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1286"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="843"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="593"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="612"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="638"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1211"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1291"/>
            <end position="1300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1562" mass="171631" checksum="94BA480F580282FA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9P2</accession>
    <name>A0A8H6C9P2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein SDS23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein sds23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_005066</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in DNA replication and cell separation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SDS23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148676.1">
        <property type="nucleotide sequence ID" value="XM_037295978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9P2"/>
    <dbReference type="GeneID" id="59333472"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030071">
        <property type="term" value="P:regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016711">
        <property type="entry name" value="Ssd23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF36">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018148">
        <property type="entry name" value="UCP018148_CBS_YBR214w"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="239"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="352"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="443"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="458"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR018148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6219241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="484" mass="52374" checksum="CA4D31E47EE2DC78" modified="2022-01-19" version="1">MRLAQQTSPSHGHRPSFTEQLRGMPPSPRGARQLSMSEAQIRELMNNPPTTGSADPKFAGRDWQHIAVGELVQPEDLRWVEVDSGVEDATNLLTESDATVLLIRANKNENSAVATFDYRDLTQYLLFATGQLQPDDEHLVVFEKLAKKAQQGHKIPLRDAKSLGNKEPFITLPHTADLTAAVETFGGGVHRIVILKEGTNHAIGILSQLRLVKFLWENGRAFPTIDQLYPREVKDLGIGSQGLISVNGDKPLKEALTLMNNEGVTSLAVVDNNYNVVGNISNVDVKLLTKSSSAPLLENTCIHFISVILSTRGMIDGQDSYPVFHINPQSTLAHTVAKLVATTAHRMWVVETPTPSSTPSSAPLTPGTGPNPHATPISIAHPPVNHPPHLSTSFPQANPPFQPLSATPSVSASAMPGQHMSGRLSGVVSLTDILNLFARASGLHPTDPEEARRKRRGSSSSSRSGVRGSMDSARSSVVDLREKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9T2</accession>
    <name>A0A8H6C9T2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004041</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149007.1">
        <property type="nucleotide sequence ID" value="XM_037294960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9T2"/>
    <dbReference type="GeneID" id="59332450"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="130"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="378"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="461" mass="50194" checksum="E8D94C2D710BED69" modified="2022-01-19" version="1">MAAGNSSRVKPRHQSHLPTSDLATQEGRNIKDMVMASDPEEAVQEPEVQPPLDPLPTSFDHGNPTSIPPPTMMPLNTAAMLSQRRRRKHSASLKRSASTPNVRGFPNGDPSMTLAEKRRNKLGYHRTSVACGHCRRRKIRCLLAPDDPQNRCANCIRLKKDCNFFPVDQQPQLERRPRTSSRAGTMASSSSDSSPALAGDHLPYSLDCTEDFNAFSQPSLSAPFTTSRGSISGLISPLTRGPIITNAFEVPLQNRPSWDSPFLDHGPTSAGHSSPGDSTHAYWGRHSGSPMTPGFSPHLPGPTGSIHSAPDARSSFTSFAPSRSDFGWSSAQRSMSFGLVEDLSLNCHNHNQNHYHPQSASMDYRRRASDMHPPSLQTSNNSSNTSISEAHMTPLSVPISSSPPQHWIAPTTWGSLPNSSLVTKGPDQWSEPTILAKVQEEDFGPLYGGEPAIIYADADLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Z0</accession>
    <name>A0A8H6C9Z0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149077.1">
        <property type="nucleotide sequence ID" value="XM_037295030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Z0"/>
    <dbReference type="GeneID" id="59332520"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052897">
        <property type="entry name" value="Sec-Metab_Biosynth_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017:SF11">
        <property type="entry name" value="ESTERASE_LIPASE_THIOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="15"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="146" mass="15164" checksum="B84F785CA53BB225" modified="2022-01-19" version="1">MKHGNRNAPKAKPTIVIVAGAWHTPIYIAPLATAPSSQSYPTVPLALPTSGNANRGLINDVAMITSRWEDVIGEQGRDVVLVVHSYGGISGSAAAGPFVRSVREKQGKDSSVVAILSMTSLAIPKDTFYAAGDRGEACVVCDGGFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA22</accession>
    <name>A0A8H6CA22_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003971</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148938.1">
        <property type="nucleotide sequence ID" value="XM_037294891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA22"/>
    <dbReference type="GeneID" id="59332381"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034335203" evidence="3">
        <location>
            <begin position="21"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="161" mass="16717" checksum="16DC584F6615620E" modified="2022-01-19" version="1">MTYKQHTMFLLFCLVGLGLALPFPPSSQANLTTSTSASLSSSSYIQTASTAAPSVSLAPSHLSAATKSVATNHTIPSQPAFPTLGPPQWSAGDISTIVFGCIASILGVLALYLTLWLGRRGPGYLTRYDVETGIGVAPDDSDLPLEDLARDTGTDSEVAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAL6</accession>
    <name>A0A8H6CAL6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149238.1">
        <property type="nucleotide sequence ID" value="XM_037294550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAL6"/>
    <dbReference type="GeneID" id="59332039"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47562">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47562:SF2">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="270" mass="29682" checksum="840CDF6D8A970CB8" modified="2022-01-19" version="1">MLIHETHQDVETFADGKGSMRIYLYHPVIPGFSQAKFPGVVIFSEIYQVTGPVARFARQMAGHGYIVAAPSSYHEFTGPEPLAYDARGTDDGNKYKIEKKVHAYDEDAALSVTALLSLPTCNGRVGTTGMCLGGHLAFRCAFDKRVAAAVCYFATDIHSKTLGAGGDDSLERVGDIEGELVMIFGKKDNHVPPAGRDLIRKTLHEEGVCFSFYEVAHAQHAFIRDELSKGRYDPFVSKVCFEMLLEVFGRALRADLGPRDGGREKIEDVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB15</accession>
    <name>A0A8H6CB15_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">rRNA-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003360</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in nucleolar integrity and required for processing of the pre-rRNA for the 60S ribosome subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
            <location evidence="2 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CGR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149663.1">
        <property type="nucleotide sequence ID" value="XM_037294282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB15"/>
    <dbReference type="GeneID" id="59331771"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005579">
        <property type="entry name" value="Cgr1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03879">
        <property type="entry name" value="Cgr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="54"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="115" mass="13451" checksum="6591A0FE07D4030A" modified="2022-01-19" version="1">MMSNSAPTASALTTPGLRKNGKQWHPQKCAFRPTSGQTSYEKRTAGRKAMTAMKAKEKEMKDEKENERQSRIQAIKDKRAAKEEKERYEKMAEKMHAKRVERLKRREKRNKLLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBG7</accession>
    <name>A0A8H6CBG7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149799.1">
        <property type="nucleotide sequence ID" value="XM_037294418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBG7"/>
    <dbReference type="GeneID" id="59331907"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="447" mass="50635" checksum="69194E5276C9B8CC" modified="2022-01-19" version="1">MAKPQQIRKKKVSAHSKIGTRHCDSSPPSSPRPTLVSDSKAPTTGRKRKQRTLPPDQQDTGDASIEHRDNQSKSSSSSTNSYRIWDRAERSYWDSLSTLRLTSDCLRELNRQNALMKTSVPKTIPAVEHPGRYGSHRNPEGVLSRPTLWSSRKASKSKPDRKSPVDEEEETGGSSAYDSNFEHHLTQNHFFRCKYHGKRPANHQEWVEAIKQPRASLSSLRMSDEYEALSRAADDARNDKGQLMIQVLPKLMGEKNSYRSGKRVKFDNLEPLTQRILDARPDYYEGHHAGLENRLLREKLRTIITPSIRQDTPFLPNFFVQINDGGGSFAVAERRARHVGALAARGMHRLQCLGRTEAYDSNAYTISAIYYAQFLNLYTHHLTQPEGSGSPPHTHMIALKSVPLFDSPESLYEGLTAIRNASEKANEYREQFIESANLRLESDTPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBH7</accession>
    <name>A0A8H6CBH7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002916</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149918.1">
        <property type="nucleotide sequence ID" value="XM_037293840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBH7"/>
    <dbReference type="GeneID" id="59331328"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF184">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN HOMOLOG 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034107888" evidence="3">
        <location>
            <begin position="24"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="499" mass="53825" checksum="5D81117178B6F2D2" modified="2022-01-19" version="1">MRRSLKILFLLLVGTLLVKQVRAPAPHPETTDSDSDSEPQPVPNTNHDDNSGSSSGEGEDLGHVGGITLSGGTPAAPVAPPAEGNEAEFEEEDVDYSEILKHVSELLDAITAIISDLDSGSSTTVSAPLPTAALPCYSVSSIYNECALRKSEFPHVVFPIQASCLCYVTKGNDTTAIWAPDSYDGHMSRCNNFAQTQSQASITNVGNNSSAFNLCSSAGDVRATPALAVPSSTTTPSATPASTTTAPSTPTATGTSGSAKRVRCHLLIRKLALEGSLRQQNVGRTTKLSGKKPLNPFLGELFPGKWEDHDGLGEAESICEHVSPHPPTSEFCIRNEKHGVQLQDYSAQRLPFAGTLSTAQYGHASLHIFNYNEDYVTTFLSIRIKGIFSTSPYSELEGETFIHLRLEAWSVIVDVIRAYEMLAAGGEKAKIGNGQRGMEVAEKAEGRRWEPRFIGRLERDGVLERLGIKDGLGEEKGGGDWRWDGEKARMVLRLFHDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBX1</accession>
    <name>A0A8H6CBX1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CT20-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003018</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150020.1">
        <property type="nucleotide sequence ID" value="XM_037293942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBX1"/>
    <dbReference type="GeneID" id="59331430"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012423">
        <property type="entry name" value="Eaf7/MRGBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13581">
        <property type="entry name" value="MRG-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13581:SF5">
        <property type="entry name" value="MRG_MORF4L-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07904">
        <property type="entry name" value="Eaf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="180"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="244"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="31475" checksum="87C00F60F9AE145D" modified="2022-01-19" version="1">MAPKKKIRQPSRAASTPVGDVVEQAEETPTTMKSDSGIGIIVTDAWTDEQETSLFKSMVRWKPVGMHKHFRMISISENLRNHGYSSPQNAHTRIPGIWEKLGSLYNLETLDAREDAFGEPIQMDGDPAEQPFCPFALPEDEYRDMMFGKRLAGEGTSSPPLLAHQLSGMSVEGGRAARRYSTVDDTDVNVPDPRSSPASISGQKISRNTRTSKGTRTSLLAEVSTIGGRQGSKGSVDQGTDDGQKDDEDDGEAMGEDENDSKAATLKDTSKASKGAAIKSNASRRSGRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBY6</accession>
    <name>A0A8H6CBY6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002448</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150203.1">
        <property type="nucleotide sequence ID" value="XM_037293374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBY6"/>
    <dbReference type="GeneID" id="59330861"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="20"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="260" mass="30053" checksum="FFF1368D7D03EBC2" modified="2022-01-19" version="1">MAFANSKQYATDADEEIIPTTTMIKNLPIHWNTGHLLHLMSQMKLPPPRALNYLYDEFDDFRGMAFATFATSEEARQVVLELNYHQVFGRKLNVQYKRKHPWVIAREDFSWRKPLQPTHSSHTNDRYKSYLHPQEKGCPVPRPTRQQTPPSESYNLLMAYQTDPVEKEKLKRILVQTGDYQEAINKFAKNRALESQEVEHPWSMQNRPILEMRPATLGDLQQIAAMESRFGLGGEASLSGLLFTNHEGDRDATDTTKEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCM6</accession>
    <name>A0A8H6CCM6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002461</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150216.1">
        <property type="nucleotide sequence ID" value="XM_037293387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCM6"/>
    <dbReference type="GeneID" id="59330874"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178:SF2">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178">
        <property type="entry name" value="MONOOXYGENASE, FAD-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22132" checksum="FC9D618F5AB4C60E" modified="2022-01-19" version="1">MAFHPDGLFAWISGIWWLGGPDLRAIDVPNVKAPETWKFQLMTSWRKSDDDEKIETADVGLADVKMRTKDFADPFRSANAWIPKGTPVYTNRVTYWQPVPWDNLGGMMSLAGDATHPMTFRLNHAIADVAKYVAALRTMKLDQEKLKDAICDYENEMIARGGEEVGLSKMNTEMVHQWDKLMQSPLFQKGAKRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCP6</accession>
    <name>A0A8H6CCP6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002556</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150311.1">
        <property type="nucleotide sequence ID" value="XM_037293482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCP6"/>
    <dbReference type="GeneID" id="59330969"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050815">
        <property type="entry name" value="TF_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338:SF10">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="596"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="658"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="728"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="611"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="729"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="739"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="856" mass="95615" checksum="644CDB1273F20B35" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCY6</accession>
    <name>A0A8H6CCY6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial glycine transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Solute carrier family 25 member 38 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_002714</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial glycine transporter that imports glycine into the mitochondrial matrix. Plays an important role in providing glycine for the first enzymatic step in heme biosynthesis, the condensation of glycine with succinyl-CoA to produce 5-aminolevulinate (ALA) in the miochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>glycine(in) = glycine(out)</text>
            <dbReference type="Rhea" id="RHEA:70715"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family. SLC25A38 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150468.1">
        <property type="nucleotide sequence ID" value="XM_037293639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCY6"/>
    <dbReference type="GeneID" id="59331126"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904983">
        <property type="term" value="P:glycine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000103">
        <property type="entry name" value="Mitochondrial glycine transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03064">
        <property type="entry name" value="SLC25A38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030847">
        <property type="entry name" value="Hem25/SLC25A38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181:SF3">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="9"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="122"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="230"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6221033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="328" mass="35535" checksum="E49ED46793D8ED3C" modified="2022-01-19" version="1">MSKDGTQRSSSTFHFFAGLATGCVSATLLQPADLLKTRVQQSRSTSLVTTLRQILAGPNSLKQLWRGTLPSVIRTGVGSALYFSSLNALRQHVATSNLLTSTGKVQASVGTSQFSSSSLPRLSNMANMTTGAIARASAGFFMMPITVIKVRYESSLYSYKSIWSAGNAILKKEGIRGFFSGFGATAIRDAPYAGLYVLFYEQSKRRLSQVREVTPLAEISNFEGGMKSSTSLSINFLSGVLAAGFATAVTNPFDAVKTRLQLMPGRYSNMIIAGRRMVKEEGFRSLFDGLGLRMGRKALSSALAWTLYEELIRRAEMEVQHKGGATVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD93</accession>
    <name>A0A8H6CD93_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002083</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150663.1">
        <property type="nucleotide sequence ID" value="XM_037293010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD93"/>
    <dbReference type="GeneID" id="59330497"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051196">
        <property type="entry name" value="RSAD2/Viperin_antiviral"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038283">
        <property type="entry name" value="viperin_w_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21339">
        <property type="entry name" value="RADICAL S-ADENOSYL METHIONINE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21339:SF0">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT NUCLEOTIDE DEHYDRATASE RSAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01088">
        <property type="entry name" value="antiviral_proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00318">
        <property type="entry name" value="Viperin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034868799" description="Radical SAM core domain-containing protein" evidence="9">
        <location>
            <begin position="17"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="50"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="347" mass="39257" checksum="9CE6A21B93469E33" modified="2022-01-19" version="1">MYLAVVFLALLTIALSTYFLSKNTQLREKIQEDADAQILKPKEGNGEEDTKTRIPISVNYHLTRQCNYSCGFCFHTAKTSHVEPLDNAKRAMLLLKEAGMRKVNFAGGEPFMNAKFLGQLAQYCKSTLQLESVSIVTNGSKVTPTWLAEYGKYIDIMAVSCDSFDEATNVKIGRGTGKHLQKFRQLAGLCEEYGVMFKVNTVVNRYNWQEDMNAAIQAMGPKRWKCFQVLIIDEENGGDKTLRDAREFQITDEEFQFFCDKHKHNACFVPEGNGVMKSSYLLLDEYLCFLNKGVKGKTKSILEIGVKEAMKDVYWDEEGFQERGGEYSWTKPSTGGCGEGKNPELEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDF6</accession>
    <name>A0A8H6CDF6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151015.1">
        <property type="nucleotide sequence ID" value="XM_037293362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDF6"/>
    <dbReference type="GeneID" id="59330849"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05120">
        <property type="entry name" value="APH_ChoK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF15">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="1">
        <location>
            <begin position="104"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="369" mass="41894" checksum="53CF3A7B23286D8A" modified="2022-01-19" version="1">MAEVTHITKPGGDFDEWEIPADRHYTLTSTTFTKRQLRQQERYTNCKGELITVPWSRELLENERDALIFISQNTTIHVPKFLHFSLEGGVASITMEAVHGELMDDLVVTLNEKDRERLTSNVFLYINDTVLPQLHQLRSSTLGSVRGNIIPPYRLQTRARRAHYRSRKSATKDYVYCHNDLAQHNIVVNPETLQVASIIDWEFSGFYPSDFEYPFWLTTRAKRTDWGNEDPAVQRLIKLIDEPGKSKPTTALQPSLALTGFRCKRFSCAPMGGQHDSPARNIFAPLLVACEQGLHRHFESILSIHKSSFLKRIPASVPLIKDVHFPCCLILAARRNHSHLADVRIECRREYASVGAPEGAGDPVHGGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDQ5</accession>
    <name>A0A8H6CDQ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150726.1">
        <property type="nucleotide sequence ID" value="XM_037293073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDQ5"/>
    <dbReference type="GeneID" id="59330560"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00027">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="14"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="WGR" evidence="2">
        <location>
            <begin position="136"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="35175" checksum="638FA583DFC1C508" modified="2022-01-19" version="1">MQKRNRYQSSRSAMGKQPLKRFTIAITGYFGEQRSSEQMRKWIHNNGGIFAYNVSPKITHLVCSKEDFKKNVAMVQKARQLRTVKIVSWDWLEDTLMKEHPMKESEYLMAPLVKCAADNKEKKKAARKENIRKGNGYHIYQDCTKFNYDVTLARTNLLVNKNDRFALKVCYPSSPTYTPPHKRHLTPDPMITTPIFPIIPLSPRARARIHCPRAQRLQLFESHATPKYYACYAKYSSPGHAPTSEMLAPIGSPWETAWDAFQGFFELKTGKKWEERFVRMALGSEAFTYTPPKVGQPRGMFLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDX9</accession>
    <name>A0A8H6CDX9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin-related protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001726</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151193.1">
        <property type="nucleotide sequence ID" value="XM_037292654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDX9"/>
    <dbReference type="GeneID" id="59330140"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10206">
        <property type="entry name" value="ASKHA_NBD_Arp8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000201">
        <property type="entry name" value="Actin-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000232">
        <property type="entry name" value="Actin-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="714" mass="80598" checksum="9E0973CE6F26042A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEE9</accession>
    <name>A0A8H6CEE9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Activating signal cointegrator 1 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001714</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151181.1">
        <property type="nucleotide sequence ID" value="XM_037292642.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330128"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18020">
        <property type="entry name" value="DEXHc_ASCC3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18022">
        <property type="entry name" value="DEXHc_ASCC3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000198">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000231">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000009">
        <property type="entry name" value="DEAD/DEAH box helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000004">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000102">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF13">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="293"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="525"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="1154"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="1361"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="180"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1979" mass="222001" checksum="35CEC4A12D5327BB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEI3</accession>
    <name>A0A8H6CEI3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151368.1">
        <property type="nucleotide sequence ID" value="XM_037292829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEI3"/>
    <dbReference type="GeneID" id="59330315"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00043">
        <property type="entry name" value="CYCLIN_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="352" mass="38813" checksum="26E610459E4C65BA" modified="2022-01-19" version="1">MAHNTTRYSSESEVFGLPLLSSSPYNLVVVNLAPRKFIKAKGNQENTIKTSTMSSQDISHSQREMSTQNSISDQDNFTLAGEIANISTENAVVAPSLTGSVHERHVIKLTIAYNEMDVLVKSLSASHEVAGYAKLLYSEVYNFSDFEELQLQHNAIVAACVHIACGEKNQQRAMPEVFRSSHATTQQMLIVSGKLKDFFATPLVRESLLSEEDKRLQSAYDALASISDPIKIAPIVVYYAKRLCKKVCNSGSFKDEEESLTVPWCLLIASRQLNDPRSFEEIFTILPGATKADMGRTGESLKVFFAAEDRQETTEASQTQGTIMGAGADARSTGKVVRTLQELIVQLNTEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIA8</accession>
    <name>A0A8H6CIA8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153072.1">
        <property type="nucleotide sequence ID" value="XM_037301438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIA8"/>
    <dbReference type="GeneID" id="59338976"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF18">
        <property type="entry name" value="IQ CALMODULIN-BINDING MOTIF DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G08920)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="453"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="937" mass="104894" checksum="D74CF5EDAB31952B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIB6</accession>
    <name>A0A8H6CIB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SprT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152966.1">
        <property type="nucleotide sequence ID" value="XM_037291531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIB6"/>
    <dbReference type="GeneID" id="59329011"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23412" checksum="E73E5013D17E1A2F" modified="2022-01-19" version="1">MPTKSGKELYREAFLKVKADRGTPYDVSQEELDAMSTFSDRNFGPFVSQTQATKEVVDHNKPSRKPSSAELAAIQRLRSALKDSLWSPDIFQWRTPDAFVTHVQPEDFALGATVGTGRVGQMSIMLHAHNLLLRDVIRDPWMHMWSTALHEMCHAYEGVRAQTGMRAGHQMDFGFKVQAVHERAQGLFGLWAIVPAEELARWGLYQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIU5</accession>
    <name>A0A8H6CIU5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fucose-specific lectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010807</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the fungal fucose-specific lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153292.1">
        <property type="nucleotide sequence ID" value="XM_037301658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIU5"/>
    <dbReference type="GeneID" id="59339197"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.70">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012475">
        <property type="entry name" value="Fungal_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07938">
        <property type="entry name" value="Fungal_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89372">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="50932" checksum="CB4F0C68D2C1EE24" modified="2022-01-19" version="1">MVNPNGYHFSNTEYYSTLQVVPQPQRTPAWIDGLFEEKESLQVWPESTLEPVKARLFSDPSDAPELSPYNSPLASSIAPDASLTRSFGTLITEKKGGAWKGGRTICGIPRKTFWILLGVKLLMMALVIAVPVGLFVRRGKHTSAHANASNVPPSSPIMISNTSSLASIAWNDTNGIMQYRVYWQGEDNIIRESAWNATANLWQLSNSAIGIAKANSPLAAVVSGPAVYPFQIHLGAISASDTIVFWLCTDPELVDGETNFTSGPFIDPTNAPAAHTALAATWDRALDCNGCSETLLLLYQDTAGQLALGNLTVDGWLWSTLFANPVPGTGLALDVRSMGNVTRNIAIFYQLGNDYLTATLFNGTTYQWETRESDPIGMIAANASIASFNYGSTSVGVTLDEVVLSTGSNGTAVNIWDGQTQTFGTGEYSTVLGESRSLATNADAHAYGLQHGTVKEFKIDNMLAWSVVGNVTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIZ3</accession>
    <name>A0A8H6CIZ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010655</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the UQCRB/QCR7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153141.1">
        <property type="nucleotide sequence ID" value="XM_037301507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIZ3"/>
    <dbReference type="GeneID" id="59339045"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1090.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1090.10">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003197">
        <property type="entry name" value="QCR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036544">
        <property type="entry name" value="QCR7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02271">
        <property type="entry name" value="UCR_14kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000022">
        <property type="entry name" value="Bc1_14K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81524">
        <property type="entry name" value="14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="125" mass="14563" checksum="E7789BF791CD6215" modified="2022-01-19" version="1">MSAPSLAGYIVKRPWLKRWMMPLANWYADAAGYRKLGLRADDLIPEESDTVLLALKRLPPKEAYDRVFRLRRAFQCSLAHQLLPKDQQTKPEEDYAYLSPVIKEIEAESRERDDLEAMVVSKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ72</accession>
    <name>A0A8H6CJ72_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_011066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153549.1">
        <property type="nucleotide sequence ID" value="XM_037301915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ72"/>
    <dbReference type="GeneID" id="59339456"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22997">
        <property type="entry name" value="GT_LH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36587">
        <property type="entry name" value="EXPRESSION SITE-ASSOCIATED GENE 3 (ESAG3)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36587:SF2">
        <property type="entry name" value="EXPRESSION SITE-ASSOCIATED GENE 3 (ESAG3)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="626" mass="69376" checksum="ECA6E0114212EA2A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJA6</accession>
    <name>A0A8H6CJA6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2293 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153251.1">
        <property type="nucleotide sequence ID" value="XM_037301617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJA6"/>
    <dbReference type="GeneID" id="59339156"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018744">
        <property type="entry name" value="DUF2293"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38113">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38113:SF1">
        <property type="entry name" value="DUF2293 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10056">
        <property type="entry name" value="DUF2293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2293" evidence="2">
        <location>
            <begin position="197"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="902"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1001" mass="114469" checksum="AEFA0E2D00B9D9C9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK15</accession>
    <name>A0A8H6CK15_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Succinate/fumarate mitochondrial transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010179</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153851.1">
        <property type="nucleotide sequence ID" value="XM_037301034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK15"/>
    <dbReference type="GeneID" id="59338571"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005469">
        <property type="term" value="F:succinate:fumarate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000021">
        <property type="entry name" value="SFC1p Mitochondrial succinate-fumarate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF2">
        <property type="entry name" value="SUCCINATE_FUMARATE MITOCHONDRIAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="15"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="116"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="220"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="35204" checksum="085C990D0A5579F7" modified="2022-01-19" version="1">MSAKSVKGPGAKKPASAATNLIAGGAAGMMEALACHPLDTIKVRMQLSRRKRAPGAKPRGFYTTGKEIVNRETPLGLYKGLGAVVTGIVPKMAIRFTSYEWYKQLLVSKDGHVSGEATFFAGLAAGVTEAVAVVTPMEVIKIRLQAQHHSMADPLDIPKYRNAAHAAYTVLKEEGIGAMYRGVSLTALRQGTNQAVNFSAYTKFKSFLQNYQPAYHDKELPSYQTMFIGLVSGAMGPLSNAPIDTIKTRLQKTPSQPGETAISRVKVIASDMFKQEGFHAFYKGITPRIMRVAPGQAVTFTVYEYLKGWLESTNFAIVGGKYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKY1</accession>
    <name>A0A8H6CKY1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154249.1">
        <property type="nucleotide sequence ID" value="XM_037300401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKY1"/>
    <dbReference type="GeneID" id="59337935"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="305" mass="34105" checksum="4B9E8E2E01E5BD1B" modified="2022-01-19" version="1">MLTDRKPNPLVVIGAEDENETGWVDFRRRIEPLLGSAPCDIDLTIETETLKELSGAVYDSKQPVDEDASTVPVDIEDQEIVVSILEITDTSRLERRPDIHDGLALKKFWDEGKPANVKVRTYMAEQGGDLAAGVMAALGSSLDLDPRLFQSGLRGHKRVLAPSDHHRAPFTSIKFGVPKLSTPLMTDAEKFKVTFYVKPDDDVGSPFFTISPLLRLNCFCWGRVITSIDDKDRRINGVSDTTVGHAEGIKKSLSLVQRGGSLGWKGEDVRLVKETKNALEEDFKRVVRETELLWETREKMASNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLK5</accession>
    <name>A0A8H6CLK5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_009461</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154170.1">
        <property type="nucleotide sequence ID" value="XM_037300322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLK5"/>
    <dbReference type="GeneID" id="59337856"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF21">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="10">
        <location>
            <begin position="101"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="634"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6225461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="783" mass="85517" checksum="3B865AFEC02A090E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLN9</accession>
    <name>A0A8H6CLN9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor spt8 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009745</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154452.1">
        <property type="nucleotide sequence ID" value="XM_037300604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLN9"/>
    <dbReference type="GeneID" id="59338140"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057544">
        <property type="entry name" value="Beta-prop_SPT8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23798">
        <property type="entry name" value="Beta-prop_SPT8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Transcription factor spt8 beta-propeller" evidence="5">
        <location>
            <begin position="156"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="285"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor spt8 beta-propeller" evidence="5">
        <location>
            <begin position="538"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="358"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="707" mass="75238" checksum="914441A5FE577A91" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLR8</accession>
    <name>A0A8H6CLR8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154563.1">
        <property type="nucleotide sequence ID" value="XM_037300715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLR8"/>
    <dbReference type="GeneID" id="59338251"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622:SF10">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="22"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="582" mass="65757" checksum="473548502F5FE455" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLV1</accession>
    <name>A0A8H6CLV1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alpha-1,3-glucan synthase</fullName>
            <ecNumber evidence="2">2.4.1.183</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_009611</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[(1-&gt;3)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-alpha-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19749"/>
            <dbReference type="Rhea" id="RHEA-COMP:11150"/>
            <dbReference type="Rhea" id="RHEA-COMP:11151"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28100"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.183"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.183" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154320.1">
        <property type="nucleotide sequence ID" value="XM_037300472.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338006"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047657">
        <property type="term" value="F:alpha-1,3-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070600">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11323">
        <property type="entry name" value="AmyAc_AGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000058">
        <property type="entry name" value="Alpha-1,3-glucan synthase Ags1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000052">
        <property type="entry name" value="Alpha-1,3-glucan synthase Ags2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058659">
        <property type="entry name" value="Mok11-13/Ags1-like_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058656">
        <property type="entry name" value="Mok11-13/Ags1-like_GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058657">
        <property type="entry name" value="Mok11-13/Ags1-like_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058658">
        <property type="entry name" value="Mok11-13/Ags1-like_Ig_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058655">
        <property type="entry name" value="Mok11-14/Ags1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058654">
        <property type="entry name" value="Mok11-14/Ags1-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47182:SF2">
        <property type="entry name" value="CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47182">
        <property type="entry name" value="CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26127">
        <property type="entry name" value="12TM_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26122">
        <property type="entry name" value="CBM_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26108">
        <property type="entry name" value="GH_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26114">
        <property type="entry name" value="Ig_2_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26111">
        <property type="entry name" value="Ig_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034134248" description="alpha-1,3-glucan synthase" evidence="9">
        <location>
            <begin position="26"/>
            <end position="2561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2171"/>
            <end position="2188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2195"/>
            <end position="2214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2226"/>
            <end position="2249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2261"/>
            <end position="2282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2302"/>
            <end position="2328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2351"/>
            <end position="2371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2383"/>
            <end position="2404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2416"/>
            <end position="2436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2460"/>
            <end position="2480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2487"/>
            <end position="2506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2533"/>
            <end position="2553"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="10">
        <location>
            <begin position="69"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1672"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1796"/>
            <end position="1815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1823"/>
            <end position="1917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1930"/>
            <end position="1994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1749"/>
            <end position="1766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1874"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1947"/>
            <end position="1963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6225611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2561" mass="284847" checksum="E3D89AD5E9537CAA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLX6</accession>
    <name>A0A8H6CLX6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009779</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154486.1">
        <property type="nucleotide sequence ID" value="XM_037300638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLX6"/>
    <dbReference type="GeneID" id="59338174"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="391" mass="41199" checksum="A2476EE8A832DBB3" modified="2022-01-19" version="1">MGHPQPAMLKQRTLSSSGNYESPFPTSTGLPATSAQLLGSGNDLSVMAGQTIVMITLAHDRHGPGSGSLPTGTDTITMSSLTLSSANTMSAPAASNTDDCGVYLLFGDFGQQLRFGRVGTVPSFTRISLVRVSQVFEVSAIATALSKDDWGGTFYVDKDVKSITALRLVFAFLQIIIGIGAALAGLGPAVEDAIASGGVALFGDPYLPRMIHIGLEVFRLPMPTFASEDNTFQKLANLGGILGEIVVGAMEGFTSANNELMEGNNYDGTGDIRSYSAGGAPYEVAGYDYDNNTGCNRVRPALESQWANPGAQGASWEGTFTIPVCDVSTAIEADYQDKQYILQPYIGDNSRPLWCGPVCGGNLTTTQAFNAAAQMTKFDSPKQACSWSPGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM10</accession>
    <name>A0A8H6CM10_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154663.1">
        <property type="nucleotide sequence ID" value="XM_037299837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM10"/>
    <dbReference type="GeneID" id="59337370"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="219" mass="25266" checksum="93CC0EBFB269D183" modified="2022-01-19" version="1">MDNWHDYQYVRGNIDSVVSFLTHKNPSSYPTNWYCDDDWLMYTKNEVGADGKENPNPPLAAEKENWKSKRLRNGQYFPGLLWDKLRATGYYPPSEEDPYHTRCNAIVNGKLFTYGYVASYNDKGIGHRTRWNLSLCPSASSNSPVSYYWEPGSGTKAAPELLKVYMRSISVTTAHELFHLTSDLETEKPWNVFDEEDDDVALELSVMEEYDGRDIRFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN39</accession>
    <name>A0A8H6CN39_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155031.1">
        <property type="nucleotide sequence ID" value="XM_037300205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN39"/>
    <dbReference type="GeneID" id="59337739"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="528" mass="58910" checksum="32B327C2D1FA09C1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNS9</accession>
    <name>A0A8H6CNS9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155182.1">
        <property type="nucleotide sequence ID" value="XM_037299181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNS9"/>
    <dbReference type="GeneID" id="59336711"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40635">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40635:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="272" mass="29790" checksum="AE3479FA4EA0EE49" modified="2022-01-19" version="1">MPPIRRYLRITQYSVLECRIYLDNPADTYRWLLNSRNPVLPRVIEAVRPLVLPKLREENAKAKSGKAGKRKGWKDVVVEDDFEVAVFLTETSSRHSILRKMKTAKTEKGRLGTTDGRLTGTRDAPVEVGEGPPGLITEESEESEGVRMADLPSAAGGEADERVTLAKEESQEGLFVSDGSDEGNVGAQETPSAKNKSKGSVGEEEDGGEDDKKKLGMDTTYDGFSIYGRILCLVVKRRGIAKGKEMAGGAGQAMMEEWITSTQMGEGQMMDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNV2</accession>
    <name>A0A8H6CNV2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutathione S-transferase kappa</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008571</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GST superfamily. Kappa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155438.1">
        <property type="nucleotide sequence ID" value="XM_037299437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNV2"/>
    <dbReference type="GeneID" id="59336967"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000096">
        <property type="entry name" value="Glutathione S-transferase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051924">
        <property type="entry name" value="GST_Kappa/NadH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014440">
        <property type="entry name" value="HCCAis_GSTk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42943">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42943:SF2">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006386">
        <property type="entry name" value="HCCAis_GSTk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DSBA-like thioredoxin" evidence="6">
        <location>
            <begin position="6"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR006386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="224" mass="24804" checksum="B32794F986172F5B" modified="2022-01-19" version="1">MAKPKLVLYVDVVSPFAYLAFHICYTSPVFKGCDVTYVPVFLGGIMKATGNRPPLQIKNKGEWIDKERLRWAKSFNIPMAEGNPPGFPANTIKVQRALTLVGILHPQRLAESLAAVFHASFAEHRQVHTPESLTPIFEKIFGEEGTKEILTKSTSDEVKQLLTAKTNEALAEGSFGLPWYVATNAEGEKECYWGFDHIAQVTDHLGLERPGSDSISGTGWRAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNW1</accession>
    <name>A0A8H6CNW1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008408</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155275.1">
        <property type="nucleotide sequence ID" value="XM_037299274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNW1"/>
    <dbReference type="GeneID" id="59336804"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF41">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="273"/>
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        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
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        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
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        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
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        <location>
            <begin position="419"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
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        <location>
            <begin position="499"/>
            <end position="515"/>
        </location>
    </feature>
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            <begin position="531"/>
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        <location>
            <begin position="635"/>
            <end position="692"/>
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            <end position="598"/>
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        <location>
            <begin position="669"/>
            <end position="686"/>
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    </feature>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
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</entry>
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        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <begin position="1"/>
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            <begin position="60"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="94" mass="9705" checksum="870FE0E4C3AEF010" modified="2022-01-19" version="1">MSNSGNKGVFSNNIGSSVGGFTKRKSGDAGSDAGSGNTAERRRSSVTKFAGLNSQKRNSQDASAAARKASFAEQNKAPGFLGGMWQNWTKGSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQX9</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <location>
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        </location>
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            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQZ8</accession>
    <name>A0A8H6CQZ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="7" type="ORF">HO133_007768</name>
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        <name type="scientific">Letharia lupina</name>
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            <taxon>Pezizomycotina</taxon>
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        </lineage>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228040.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037298638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQZ8"/>
    <dbReference type="GeneID" id="59336165"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000053">
        <property type="entry name" value="SNF2 family helicase/ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF14">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="421"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="960"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="363"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="814"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1150"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="380"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="822"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="921"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1174"/>
            <end position="1191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1191" mass="131651" checksum="BEB6C3F67DAB8600" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR05</accession>
    <name>A0A8H6CR05_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007783</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155989.1">
        <property type="nucleotide sequence ID" value="XM_037298653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR05"/>
    <dbReference type="GeneID" id="59336180"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="410"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="513" mass="57410" checksum="3787BDE1669991E3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR20</accession>
    <name>A0A8H6CR20_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NAD dependent epimerase/dehydratase family protein-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007721</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMP52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155927.1">
        <property type="nucleotide sequence ID" value="XM_037298591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR20"/>
    <dbReference type="GeneID" id="59336118"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051170">
        <property type="term" value="P:import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000366">
        <property type="entry name" value="Protein FMP52, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014843">
        <property type="entry name" value="Him1/Fmp52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097:SF7">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08732">
        <property type="entry name" value="HIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="24793" checksum="79C2815245DE2BF3" modified="2022-01-19" version="1">MSSAALVGCTGLVGSHILTTLTSLSSHPTIHAIARRDLPTSSANVHPLIEPDSSKWPSSLQSLSPTPNIFLSGLGTTKAQAGSFEAQRKVDYDLNVSLAKAAKESGVKVYVLISSAGVSTKSLFPYGKMKAELDEAVQELGFPHTILVKPGLLVGPRKDSRPMEAAFRVVAKGLGAISKGWLTDWWAQDADVIGRAAVAAGMQCVEGKRDEGVWVVSQNDIIRLGRTEWKGET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR34</accession>
    <name>A0A8H6CR34_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155961.1">
        <property type="nucleotide sequence ID" value="XM_037298625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR34"/>
    <dbReference type="GeneID" id="59336152"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="28107" checksum="EC1B57C3DED63F27" modified="2022-01-19" version="1">MATDGATMTFFAFPREIRDLIYTFLTPKDKTYTITSANTVALAKAFSTLAPDLGIINTSKTVQYEMLETLCPNNTFRFSFPGSDTKTQSMDLELAELMTHIEIYIDLEHFHWERFRVGRHDHEIHGISGQVCEVLRVWNGLSVRRETCRITIKDGISFISPLLRLPFLDAVKTLVGLDTLLIIVLKFKWDLRKLGAVLGPSLGPSTVHYRWPLLEYVDRDYGCIKFHPRRSLAERLEQAAGENMAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR45</accession>
    <name>A0A8H6CR45_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="10">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_007771</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for PP2A-like phosphatases modulating their activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2Ai) in presence of ATP and Mg(2+) by dissociating the inactive form from the complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155977.1">
        <property type="nucleotide sequence ID" value="XM_037298641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR45"/>
    <dbReference type="GeneID" id="59336168"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0697">Rotamase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="375"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="425"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="465"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="503"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="531"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6228043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="543" mass="57942" checksum="6B6E35E9ACA4F05E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRC6</accession>
    <name>A0A8H6CRC6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156399.1">
        <property type="nucleotide sequence ID" value="XM_037299063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRC6"/>
    <dbReference type="GeneID" id="59336592"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034520540" evidence="2">
        <location>
            <begin position="25"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="276" mass="28751" checksum="4DAE0C3884701C86" modified="2022-01-19" version="1">MCCRKFFLLLLATIFALICPPINGASLEKRPYDMLDNPGAVRRGAASPPDVNSTSVMSNETTAAAPAAAATPETAATSANSALLATPATFTGQSQITAAPPPPSGSAGCVPWATCNLFYPYVNVYYWPEDPQNTACLSKGPQITTPSPDVTNIQSPSVYVNFPSIRAHDGCSQIGATFTNLITSFAPSALSTIGGDSKTYSFNFGDLPCPPAIIGWNSSQGPYAPLLAPPEFLFHLDPAFLTCIHGASQGIDPYTALVTTNAASGPGKPGCRRPCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRH8</accession>
    <name>A0A8H6CRH8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxycytidylate deaminase</fullName>
            <ecNumber evidence="7">3.5.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">dCMP deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_008074</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.12" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156278.1">
        <property type="nucleotide sequence ID" value="XM_037298942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRH8"/>
    <dbReference type="GeneID" id="59336471"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000035">
        <property type="entry name" value="dCMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002333">
        <property type="entry name" value="Deoxycytidylate deaminase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086:SF18">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="10">
        <location>
            <begin position="176"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6228344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="328" mass="36936" checksum="BC41985FFB607B20" modified="2022-01-19" version="1">MLVGLCGGICAGKNSVAEYLTENVGFVRLHLALQPSPLLEVEQHDAKENSFACVQSLLDFVTKQWQQRWVMTDVWDENILENLLRRPSFILVSVDAPVSMRWKRLKNRCEQQNMASPTLEEFVFRNDDHLYNSKIGLACLIDRAEVRLVNQTQSIDTLHETLRDLDLANEQRLRPNWDQYFMQLASLAAQRSNCMKRRVGCVLVREKRVISTGYNGTPRNLKNCNEGGCARCNAGHKAGVSLSTCRCIHAEENALLEAGRERIREGSILYCNTCPCLTCSIKITQVGISEVVYSQGYGMDNETAAVFVEGGVKLRQFSPPRKGLVHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRK9</accession>
    <name>A0A8H6CRK9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156319.1">
        <property type="nucleotide sequence ID" value="XM_037298983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRK9"/>
    <dbReference type="GeneID" id="59336512"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="210" mass="23819" checksum="0A64F240E7A849A1" modified="2022-01-19" version="1">MSRVYNVNNPDPRRAPDLAPRPTFGLWDAIFFLDQHGTSLSQADEVIFGHWDDYADHTVNILHQMIKQTEGLMFSDDEYTDLVRTFQAFPHRPNLSNVLRDNMYDEHECIVKTLADLAQTPVQKVWHMWATRVEEVHQGLAEKMNRCKLQLAQQGEGDFGLANLSLSSNLPKAESLTGLLHKMLDSASEPDNAQDNPATGLEAHMQQMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRT1</accession>
    <name>A0A8H6CRT1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_008039</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156243.1">
        <property type="nucleotide sequence ID" value="XM_037298907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRT1"/>
    <dbReference type="GeneID" id="59336436"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000059">
        <property type="entry name" value="Inositol phosphosphingolipids phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF24">
        <property type="entry name" value="PHOSPHODIESTERASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="416"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="14">
        <location>
            <begin position="14"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6228309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="477" mass="53693" checksum="0FC0CFE9C0F02A9A" modified="2022-01-19" version="1">MAAFSEAPNTLNVISYNCLGLKYISHYRRERLAEIGRRLALASPIPEIVGLQECWTQEDYQSIRTQTKHILPYGKYYYSGIFGGGLAILSKWPIEESSMVRYPLNGRPTAFFRGDWFVGKGVACARIRLGPRLEDVAEVFCTHLHAPYESEPNDSYLCHRTAQAWEVAKLMRGAAERGHLVLGLGDFNMVPLSFAHQLISGYGSVRDAWRVLHPESSIGSANDNLERRRGRGIPTANYNLSENGATCDSVLNTWRWSKELQKRQYRGDNIIVDDTTQDPRAKRLDYIFIGDSDYLRSPSNNKWHVQDIKVGMTDRHPLIGCSLSDHFSVEATLSRREQPADLSLSTSHAGSGFYTSQQIDIDSLSIPTYDIILSMIETYARRERHQRHFRLSNFGVSIVVAVGCFTAVWWSPRPFVAFLLILLSTLGFGAGVLDGLIGGLFIGSELRALKEFEWEIQNAKEMAEGTRGGDQENVKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7B8</accession>
    <name>A0A8H6F7B8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147375.1">
        <property type="nucleotide sequence ID" value="XM_037297250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7B8"/>
    <dbReference type="GeneID" id="59334753"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034678524" evidence="1">
        <location>
            <begin position="22"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="335" mass="36698" checksum="E4F1BADDD35FC50D" modified="2022-01-19" version="1">MGKFSKQPFFQLICLLSLARAIPSPLTLPADTLLEDSFGNSLPNSTLAAVIPNDFTSKPTLPLDEPVLNRQNTLMLTLHALSNLALDDFDGEQQSQTWRSLQHVSIDILGPSMVVESALAIRKYAVWGIYKAVHLMVASNDFRSRNYELYWQGTLVGFVGFNTGAYGALSIGNATTNGTGQGVQEGSLSVSLNTSSDATTTDLGNSRTTLSFELNGRAIGESNVYMTLFTGILKAAPYSKDERVQDLFFVNSRAFNTYLSFKEINDPGPDGPYFEYQQLIQLFTQLPSWVISHSSQWTEAYMLVSVDGRPVGAGVLNWQIRQKTDAAHGDSVVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7F4</accession>
    <name>A0A8H6F7F4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006427</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147450.1">
        <property type="nucleotide sequence ID" value="XM_037297325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7F4"/>
    <dbReference type="GeneID" id="59334828"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053178">
        <property type="entry name" value="Osmoadaptation_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111:SF6">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G01940)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="455"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="474"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="503"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="610" mass="67580" checksum="56DAEE4FD6548D11" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8J7</accession>
    <name>A0A8H6F8J7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005489</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148381.1">
        <property type="nucleotide sequence ID" value="XM_037296399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8J7"/>
    <dbReference type="GeneID" id="59333895"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03755">
        <property type="entry name" value="proteasome_alpha_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="240"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="269" mass="29181" checksum="F5675B1AAC92A83E" modified="2022-01-19" version="1">MASGYDRALSVFSPDGHVFQVEYALEAVKRGTCAVGVKGKDIVVLGCEKRSAMKLQDTRITPSKIGLVDTHVCLAFAGLNADARILVDKARLEAQSHRLTVEDPVTIEYITKYVAGVQQRYTQSGGVRPFGISTLIVGFDKGDTTPRLYQTEPSGIYSAWKANAIGRSSKTVREFLERNHKDDMDREETVKLTVKSLLEVVQTGAKNIEIAIMAPGKTIEMLPVEDIESYVKSIEAEKQEEAAKKKTGRTPGTGTAAILTRSDAGASGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8S1</accession>
    <name>A0A8H6F8S1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005471</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148363.1">
        <property type="nucleotide sequence ID" value="XM_037296381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8S1"/>
    <dbReference type="GeneID" id="59333877"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="29"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="189"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="286"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="330"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="387"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="439" mass="48950" checksum="309DA21864596546" modified="2022-01-19" version="1">MENGGDHELGMTIDEEREEEQMEEKIVNEEYKTWKKNAPFLYDLMLSTALEWPTLTTQWLPDKQEIPEKDYATHRLLIGTHTSNEAQNYLQIAQVQLPKPVTPDAADYDEDREEIGGYGGGASKNSPVMEVKFNIVQKINHPGEVNKARYQPQNPNIIATLCVDGRVMIWDRTKHPSQPTGNINPQMELLGHDSEGFGLSWSPHQAGHLATGSEDQTVRLWDITKFTTSNKALHATRTYTHHTAIVNDVQHHPLHASLIGTVSDDLTLQILDTRSPSTASSATQAVDGHQDAVNALSFNPASEYVLATGSADKSIGIWDLRNLKSKLHALVGHNDSVTSLAWHPFEEAILGSSSYDRRVIFWDLSRVGEEQTPDDAEDGPPELLFMHGGHTNRIADFSWNMNDPWVLCSAAEDNLIQVWKVANAIVGKDADDVPIEELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8T6</accession>
    <name>A0A8H6F8T6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003891</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148860.1">
        <property type="nucleotide sequence ID" value="XM_037294813.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332302"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF26">
        <property type="entry name" value="FAMILY HELICASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G09120)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="2329"/>
            <end position="2492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2115"/>
            <end position="2204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2483"/>
            <end position="2503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="243"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="277"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="292"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2115"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="2163"/>
            <end position="2172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2534" mass="284380" checksum="6052D0D4F0483B24" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F926</accession>
    <name>A0A8H6F926_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148663.1">
        <property type="nucleotide sequence ID" value="XM_037295965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F926"/>
    <dbReference type="GeneID" id="59333459"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="253" mass="28867" checksum="4AF358EA7ACF6088" modified="2022-01-19" version="1">MASSLEPAQRPINLIESADRCEQHLNSLRKYANNSKTKHNSGKKKVLLEDVITRPEGRQTSDEEIKASVTSVFEDIVSRNRAVEVAHLWKELDEIRDPIGYLHNQVKTVLRETLDQKERRTRISEIVQRRSKSYYVFVNAIGQMSEAERRSTRFGTYYLLWREFSPGLDLNGNVGNADDPGDGKIYDQGSIQQASQILKAFYDAWSEPIIKANVANPTSPDILRKAGKIPEMAKICLILRGIKQERLLDCFCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9I2</accession>
    <name>A0A8H6F9I2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003306</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-25S rRNA processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM RBM34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149610.1">
        <property type="nucleotide sequence ID" value="XM_037294229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9I2"/>
    <dbReference type="GeneID" id="59331717"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="315"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="134"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="467"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="426"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="535" mass="58785" checksum="8ACCA2A320806044" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9J6</accession>
    <name>A0A8H6F9J6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003836</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149446.1">
        <property type="nucleotide sequence ID" value="XM_037294758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9J6"/>
    <dbReference type="GeneID" id="59332247"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="559"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="592" mass="61877" checksum="CB1CA258ECC954D7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9U2</accession>
    <name>A0A8H6F9U2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002938</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149940.1">
        <property type="nucleotide sequence ID" value="XM_037293862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9U2"/>
    <dbReference type="GeneID" id="59331350"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015355">
        <property type="term" value="F:secondary active monocarboxylate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035879">
        <property type="term" value="P:plasma membrane lactate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000190">
        <property type="entry name" value="Sugar transporter family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000276">
        <property type="entry name" value="Sugar transporter family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="62"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="521" mass="57480" checksum="1C592E34E04362D4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAU3</accession>
    <name>A0A8H6FAU3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001551</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151020.1">
        <property type="nucleotide sequence ID" value="XM_037292481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAU3"/>
    <dbReference type="GeneID" id="59329967"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF41">
        <property type="entry name" value="XENOBIOTIC COMPOUND MONOOXYGENASE, DSZA FAMILY (AFU_ORTHOLOGUE AFUA_3G15040)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="3">
        <location>
            <begin position="37"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="503" mass="55882" checksum="7F307A5A08FEF93B" modified="2022-01-19" version="1">MGESKKQWILNAFAMQAPGHLNPGLFKYPKDQGRKYKDIQHWIALAKELEQAKFHAIFFADVLGGYDVYKGPANLDPTIPAAAQFPINDPLYTIPAMAASTNSIGFGVTASTTYDAPYALARRFSTVDHLSNGRVAWNIVTSYLDSAARNFALKTQIEHDERYRIAHEYLHVTYKLWEGSWRDDAVLPPPSPAEGEDPQGNNNAYADPKAVRQVNHQGEYFTVPGPHLCEPSPQRTPFLFQAGTSTAGRKFAATHAEAIFLNGHTPELVRPSVDGIREEASRLGRDPQDIKIVAGLLVIVAESDEAARKKYEELASYGDREGALALFGGWSGYDLSKYSDEEDFRFSKEAPPAVRSMVNHWASTSPEGLTWNKKTIAEWLVLGGNGAKVIGDPTTVADELERWIEVADVDGFNLSYASIPETFQDIITLLIPELRRRSVFWEDYAVPGGTLRENYFARPGGNRLADNHPGAQYFWKAGELEPAYAIRARSEKESNDDKNDVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB15</accession>
    <name>A0A8H6FB15_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002064</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150644.1">
        <property type="nucleotide sequence ID" value="XM_037292991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB15"/>
    <dbReference type="GeneID" id="59330478"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19677">
        <property type="entry name" value="UBR-box_UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040204">
        <property type="entry name" value="UBR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047506">
        <property type="entry name" value="UBR7-like_UBR-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="6">
        <location>
            <begin position="56"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="4">
        <location>
            <begin position="56"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="306"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="506"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="529" mass="58129" checksum="19574E2A47FD5EAF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB35</accession>
    <name>A0A8H6FB35_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151006.1">
        <property type="nucleotide sequence ID" value="XM_037293353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB35"/>
    <dbReference type="GeneID" id="59330840"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="143" mass="16036" checksum="3D7E5745E9BD848C" modified="2022-01-19" version="1">MSGYLGKRRRQSLTKNLATNYIEETTPMTALAKHVTILDAMTLEEILGPSAKRIEAIENDVLARIWVKQGEEKFNDLCVMHGGVEEVWRIKESPFVSKMPEDNLHTVGHNANTEQIVLDLARFGMAVGKFVDSVSEGVSMSMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB99</accession>
    <name>A0A8H6FB99_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151547.1">
        <property type="nucleotide sequence ID" value="XM_037292128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB99"/>
    <dbReference type="GeneID" id="59329614"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="335" mass="37551" checksum="BF54A6EE6844927C" modified="2022-01-19" version="1">MDQASDSQQATGNHPSADWTSQLVRWPCIDTSTKALKKLACVRELTLRHRRVDLNFKPKKNQEAALGQICGELALAACRRCIESVPILSTPNGLNATSKILDEQAKPAQVANYQPGPSAVLAGPQVSQSDNNSVSTRLRSKKDDLRKPPNFSANFAFPRTVVGVTTNEPLTRKRQWQETTTTGPALGQWPPERGTDAVNQQSEETILCLREEIQRLRREKSDQQARQLALIKGFVTSAAVVEIAVDVREAELRCGLRNMSGTPEEMQEQAQQMIDFSKATSWHVSRFVNDMLEEQRVFLYDDQQKAMRLEYDKAEPSQAVTAIIDEELSFMTRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBB0</accession>
    <name>A0A8H6FBB0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Impact N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001564</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151033.1">
        <property type="nucleotide sequence ID" value="XM_037292494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBB0"/>
    <dbReference type="GeneID" id="59329980"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF26">
        <property type="entry name" value="IMPACT FAMILY MEMBER C14C8.09C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Impact N-terminal" evidence="3">
        <location>
            <begin position="26"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="284" mass="31060" checksum="A3F3501A7477098B" modified="2022-01-19" version="1">MSQKRGRSVSPASPPLVFKSSPIEDRSSTFLAFYSPTLAAKELQAHTEFKSASHRIAAWRKPSSQRSLNVQRLLDTGHDDDGEKYGGKAVEKVLMETDVEGAVVVARWYGGVMLGPMRFDHIKNAARDAIMQWAQDRERSAKKAKVREDEAEKERLIQTLPERDQSITVLRDLLAGKSQQPSSAPSGERTPAKRTDYSTLPLATLEKLERVRDATIGWILKQLEKAENTQVEEPGAKNAAIPKAPGAPKSSAHVGAPPAKEEISKIADLGSQGTEVAPPKHAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBL4</accession>
    <name>A0A8H6FBL4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YEATS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001242</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151591.1">
        <property type="nucleotide sequence ID" value="XM_037292172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBL4"/>
    <dbReference type="GeneID" id="59329658"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16905">
        <property type="entry name" value="YEATS_Taf14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016665">
        <property type="entry name" value="Sas5/TAF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23195">
        <property type="entry name" value="YEATS DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016551">
        <property type="entry name" value="SAS5/TFIID_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YEATS" evidence="4">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="227" mass="26091" checksum="E2810DB562449FD8" modified="2022-01-19" version="1">MPEVKRTLKIITEQKVTGDATLEMPLRKWNIELHLLGPHGEDIPADCFEKVVYKLHETFGQRATQTFKMPPFRIQEEGWGEFDMLLVLTPKDKGAEITVAHDLNFQSEHYETKHPIVFKNPKPALLAILKESGPVPGDANGVKARDESAKKKKRPDKGIDMEKLAENMQKMTEDDLLLVVQMIHDNKTADTWTKNDVEQGEFQVDLYTLPDSLIRQLWDFTNEKIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBV4</accession>
    <name>A0A8H6FBV4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151559.1">
        <property type="nucleotide sequence ID" value="XM_037292140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBV4"/>
    <dbReference type="GeneID" id="59329626"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14859" checksum="DE55CF24DBB43791" modified="2022-01-19" version="1">MAAAKKHIKIVKKRTKRFNRHQSDTYKCVDANWRKPKGIDNRVRRRFKGQAAMPSIGYGSNKKTRHLMPSGHKAFVVHNSRDVDLLLMHNKTFAAEISHAVSSRKRVDIVAKAKQLGVKVTNAKARVTTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC68</accession>
    <name>A0A8H6FC68_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001062</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152356.1">
        <property type="nucleotide sequence ID" value="XM_037291994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC68"/>
    <dbReference type="GeneID" id="59329480"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.100">
        <property type="entry name" value="YAP1 redox domain. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF8">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="4">
        <location>
            <begin position="116"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="54"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="181"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="314" mass="35110" checksum="FEBDBA4AED00FBF1" modified="2022-01-19" version="1">MDYSYFAQPAQPYQFFGIAPAKPELSYTPQEEPHNDPLDIYDPNTFQFFDQNAHFNPPAIIPSTQSPPLSIPQSDSLPNPLPLPYDRSLSQDLTGDVDLDQAPGRGSSDDDKDNLTPAQSRRKAQNRAAQRAFRERKERHVKDLEIKLKSMDAHSTDLMTENERLKRELDRIATQNEILRATTTPRRAPQHEHSHNAHHEGPSSPEGLTSGPMIYSPSTFNAAFAEHHTSDSEEPISHRIEISETTGERLLATGAAWDFIQHHELYRRGLVDIADVSEKLKEKVVCNGAGPTFSERDVIQAVEDSVGAAGDELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC92</accession>
    <name>A0A8H6FC92_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphopantothenate-cysteine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152162.1">
        <property type="nucleotide sequence ID" value="XM_037291800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC92"/>
    <dbReference type="GeneID" id="59329284"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="44053" checksum="8C1551A41E31D79D" modified="2022-01-19" version="1">MGALDAVSSSLPPPEKNYFDQNPPPETLDFHGKLAKAFISHHLQSNRAVVLVTSGGTTVPLENQTVRFIDNFSAGTRGATSAECFLDAGYAVIFLHRQFSLLPYSRHYSHSTNCFLDYMIEGENSEVVVNPEYQKKMLGVLREYNAARRNNTLLLISFTTVTEYLWELRKISMLMRPLGPKALFYLAAAVSDFFLPRDRLSEHKIQSSETNRAGGAAEPGPSGNADTFQGKQLIINLDPVPKFLKSLVDGWAPEGMIISFKLETDPSLLVSKAQQALEKYSHHLVIGNLLSTRKFEVVFVAPGKEERWIRVPVHRRVKSVSGVRPTIDAVNNGDIPEQEWMNGDLEQEIESLIVPEVAKMHRLFTQRSQQHKEEDEDDLLAKALAWAEFGNAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD38</accession>
    <name>A0A8H6FD38_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000287</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152661.1">
        <property type="nucleotide sequence ID" value="XM_037291226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD38"/>
    <dbReference type="GeneID" id="59328706"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000121">
        <property type="entry name" value="Splicing factor 3b subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000288">
        <property type="entry name" value="Splicing factor 3b subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="13"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="101"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="249"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="256"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="297"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="312"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="350" mass="37385" checksum="54DFEC45366390B1" modified="2022-01-19" version="1">MSGARHWEQDKEATVYIGNLDERVSDSLVWELMLQAGRIVNVHLPKDRVTQTHQGYGFVEFISEEDAEYAARIMNQVRLYGKPIRVNKASADKQKTVEVGAELFVGNLDPMVTEQTLYDTFGRFGSLISAPKVARDDANLSKGYGFISFSNFEASDEAIANMNGQYLMNKEISVQYAYKKDGKGERHGDQAERMLAAQARKHNVQPTAQPLPAGLFSGGAPNAPAAMIDGDGGRPGSVAGFMNGRAVMSNGNYQNVPPPQQQRPAPPSQPLPAPPSGLPARPPPSQAGYGGPQGFLPPGFNPSQQQGFPQSGAPPPPGFGPPPGFGPPPGQGPPPGMPPGFQQPGFGRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDQ5</accession>
    <name>A0A8H6FDQ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009915</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153589.1">
        <property type="nucleotide sequence ID" value="XM_037300772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDQ5"/>
    <dbReference type="GeneID" id="59338308"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF139">
        <property type="entry name" value="PAIR-RULE PROTEIN ODD-PAIRED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="335"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="364"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="468" mass="51524" checksum="A72020907DFF0DF6" modified="2022-01-19" version="1">MSFGALDPGSGMQLSFIGRTISECTSCPSEPNVAGCCRFPANAEERQHLSECIDAGCAEPVCDECSHHEDCCDDCLVDCGSDCGTSFGCDELDVRVDDTYVFEGSHSELFGDQTAQTFGFGCRLPRLAMDNTITESGNFGKTLDDTRSSTLPAHGMNQSYPGSKIQSYPSFQTPWAPHTPLNAPEATTPSGASYDSDFLGLRNHELAPANVSPLARNGDPLTYTSDSTHDSPDPMDFSPSAHYDHVNLLTEECDPTRPFHPSLRCQWADPNGKPCGKNLRLGNDMHEHLKSAHGVKSGVFCRWVGCTVGVFGASLHKFASSVERHTWGHSGYRPYKCSACNEGFAAASVRDEHYTNIHLRKKVFSCDLCPHQCTSATNLKRHKDDKHSAERFQCEFCNRNDKRRLFPRGPNLARHFRNCKYVQAQFPEAAAAGKAKADWLPPGYKRGHHGMDRAKVTPPNYLPAQMDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEE2</accession>
    <name>A0A8H6FEE2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Origin recognition complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010002</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153675.1">
        <property type="nucleotide sequence ID" value="XM_037300858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEE2"/>
    <dbReference type="GeneID" id="59338394"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001597">
        <property type="entry name" value="Origin recognition complex subunit Orc4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016527">
        <property type="entry name" value="ORC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032705">
        <property type="entry name" value="ORC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14629">
        <property type="entry name" value="ORC4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="412"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="51"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="205"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="812" mass="88669" checksum="4ECA5969582245D4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEU6</accession>
    <name>A0A8H6FEU6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Collagen-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009757</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154464.1">
        <property type="nucleotide sequence ID" value="XM_037300616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEU6"/>
    <dbReference type="GeneID" id="59338152"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057230">
        <property type="entry name" value="DUF7908"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF3">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXV) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25485">
        <property type="entry name" value="DUF7908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="8">
        <location>
            <begin position="27"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7908" evidence="9">
        <location>
            <begin position="202"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="356"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="400"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="464"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="475"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="512"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="554"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="622"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6225755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="723" mass="72230" checksum="9314FD518E8A7D33" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFQ4</accession>
    <name>A0A8H6FFQ4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155146.1">
        <property type="nucleotide sequence ID" value="XM_037299145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFQ4"/>
    <dbReference type="GeneID" id="59336675"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2970">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="223" mass="23302" checksum="13D6A3832B655300" modified="2022-01-19" version="1">MSLQNGTGAQKVIAKALIGSILHSGVEGDGMVLVTVENNGTSNYSVEARNNLFDTNSPYEPMNVSSLAGTQVQLVGAEYPYGLLTDASFVQLPAGAIWQRELNITAYMPTDMTITKPTASCYYVTFPDGFWAIDTTGMPSGENLATEFLTPGASRLVDLYIASNILHLNVTAVPGSGATLTATTAAIPQQPAATEIVGSQTIGIASSETIGTSIDQYDNILGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGE2</accession>
    <name>A0A8H6FGE2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7907 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008593</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155459.1">
        <property type="nucleotide sequence ID" value="XM_037299458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGE2"/>
    <dbReference type="GeneID" id="59336988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057229">
        <property type="entry name" value="DUF7907"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25484">
        <property type="entry name" value="DUF7907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034462614" description="DUF7907 domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7907" evidence="2">
        <location>
            <begin position="38"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="192" mass="21026" checksum="4FD75384C3EA1F4D" modified="2022-01-19" version="1">MHFTLPVFFSFINLSLALAVTPSNPTNSTANTTFTPPSRYYLKTSVIGAGNADKNDLYVSSYHTGPGLSDATLLPFSPTSNTVGFLNDTHQQFDLHSSVPFGMIMEPSENLDVAWNFVRINELVFDAGYGSTDGFSFNASGLQWDGRGVGVFVGWLACDWWHGVPQLFWEFFFVPTGIPSSCAQVELHPVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGE9</accession>
    <name>A0A8H6FGE9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">L-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="15">1.1.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_008623</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(S)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19909"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19910"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">In the C-terminal section; belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the N-terminal section; belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.3" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155489.1">
        <property type="nucleotide sequence ID" value="XM_037299488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGE9"/>
    <dbReference type="GeneID" id="59337018"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004460">
        <property type="term" value="F:L-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02922">
        <property type="entry name" value="FCB2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000062">
        <property type="entry name" value="Cytochrome b2, mitochondrial, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037458">
        <property type="entry name" value="L-MDH/L-LDH_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF104">
        <property type="entry name" value="CYTOCHROME B2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="5 17" id="KW-0349">Heme</keyword>
    <keyword evidence="10 17" id="KW-0408">Iron</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="18">
        <location>
            <begin position="6"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="19">
        <location>
            <begin position="117"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6227181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="497" mass="54587" checksum="488FE40F396AFB16" modified="2022-01-19" version="1">MSSNPSHTLSFHDLKRHNNDRDCWIAVHSKIYDITNYLDSHPGGSSIILRYAGSDATAAYDEIHAPGIIEEALPPECYRGLLDQAEVVNLPPEQKEDMETIEKTTGQSTSTETYTKPELFKLISTQDFVDVARNTLTPKAWAFYSSAATDLVTHHWNSDVYRRIMLRPRVMRNVAKAETRRSILGCPSSAPFFISPAAMARLAHPDGELALARGCASEGLIQVISNNASYPLASVIQAGCTNQPFFFQLYVNAERAKTTQLLHKARELGVKAIFVTVDAHVPGKREADERIAAENVSSAISGAVASNDRKGGGMGRLMGQYIDKTLSWDDIPWIQQTSRLPVVVKGIQSAADAKRAVEYGVQGIVLSNHGGRSLDTAQPSMLTLLELHRICPEIFHRCEVYVDGGVTRGTDILKALSLGATAVGIGRPFLYSLCYGQEGVEHLSQILKDELETSMRLAGITDVGQAGPGLVNTRDVDYLVPGSEEHDWIRWRPKAKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGK2</accession>
    <name>A0A8H6FGK2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008790</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155654.1">
        <property type="nucleotide sequence ID" value="XM_037299653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGK2"/>
    <dbReference type="GeneID" id="59337185"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039603">
        <property type="entry name" value="Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019083">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235">
        <property type="entry name" value="PROTEIN FYV4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09597">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01238">
        <property type="entry name" value="IGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS41 SAM" evidence="5">
        <location>
            <begin position="52"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6227346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="245" mass="27469" checksum="3DC0BAEC70ABCC70" modified="2022-01-19" version="1">MIVRSLPSPLIPSTLQSFLIRSQPSHGLRHLHARASAFQPPTPQPTPFVPDASTFLKLIGRNLSQHAGKIPTWQSLFSLTSTQLRELGVEPARTRRYLLWWRDRFRKGIFGVGGDLRNVKDGGAELRVVELPVAKAAIPKSRSTSIAIGLQRTKRMIVNEPPEVIEKSLSLQQLKPVDGMKVVGLRTIVGPYVQPVKGTSGSVATIKVQEGMWEVRRGVKVDGGERRKLQVRRKRLLEERRTTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGN2</accession>
    <name>A0A8H6FGN2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155729.1">
        <property type="nucleotide sequence ID" value="XM_037299728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGN2"/>
    <dbReference type="GeneID" id="59337260"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="461" mass="50074" checksum="C946794235D914AD" modified="2022-01-19" version="1">MLAARDQENLVHGHQAAAASKPLNQGARQLAPKTPAKAPKTPFKVPLNDENNAGALGGGKGGLKTHGKGNENMILGAKKGGLGDKTAFVTPMAPRTRAPLGMKTTNAKAFQTPAPPPADNDLGKTNQKSVSARKAKPRISHAEMTKLEVLGDKDVLKEREIEYMPPKAKNLPDYPDDHVELNLSMFEGENRMRGAWSHFMTREGPDGLSHAEREEQRLQRIYDIADRKMEALMQRDIDSMPIPCTHYPECPTETCKDTVEARKAAEEQYHETIATIEAETTPATRKLIPTKGPSTLKSKVAAASLSLPKQSGPASKTASKPTFAPAKPRLTTSLVARPKNAPAPTNPSPMRHNAAVAASKTTMGYSKGRATSATLRKTVVPKKDVKTGSNEIPDTSLAPAEYIRRYGVPRVGSEMWIRCRAAGCFDEDEGPSLEEIFAGDHPHGLDTLLREEAEQEFQLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH75</accession>
    <name>A0A8H6FH75_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155955.1">
        <property type="nucleotide sequence ID" value="XM_037298619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH75"/>
    <dbReference type="GeneID" id="59336146"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="225" mass="23297" checksum="9A9AED146AD80434" modified="2022-01-19" version="1">MPGVPLDALAQVKRGLRSLFGRRKKKKQPTQPEPPPSSNAHIQTATAVAASPVEAPATSNRSVTEAEDALENEERLHPPPHPAAELTPATAVSLMPDPPPATTTAPLSSGLPASQHHIAAPSQIEEAKEIDLNPARDIKTGEGGGGGDITTESHEGKNDSSDARPKLKPDNETGMSATSGPMGDHMAEVWRDSDVGLKEGRPEGLEGEAGGLAEGAEREAGGGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI76</accession>
    <name>A0A8H6FI76_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156620.1">
        <property type="nucleotide sequence ID" value="XM_037297985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI76"/>
    <dbReference type="GeneID" id="59335492"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF8">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="152"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="617" mass="70153" checksum="08D2DD718AC38F63" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIC9</accession>
    <name>A0A8H6FIC9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156715.1">
        <property type="nucleotide sequence ID" value="XM_037298080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIC9"/>
    <dbReference type="GeneID" id="59335587"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034602421" evidence="2">
        <location>
            <begin position="19"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="113" mass="12449" checksum="943F24EAE5D87B5F" modified="2022-01-19" version="1">MRFSPMILSTLVVGYALGAPMSSSKGESYSTTSHLPWLILQAAAGIRSLQPARDISPKTRSAELEPDENMVYSEYKASRSTETRATEAKDQDDEVLYTEYSPEGTKPVGDRQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIH6</accession>
    <name>A0A8H6FIH6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007261</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156788.1">
        <property type="nucleotide sequence ID" value="XM_037298153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIH6"/>
    <dbReference type="GeneID" id="59335660"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901:SF1">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="28987" checksum="1905D579AB743E7F" modified="2022-01-19" version="1">MATAMAKRLEGKTILVTGASSGIGKSTAMEFARTSPKSLKLVLTARRIDTLKVIAEEIKEEVGSGVQVLPVKLDVSNPSEVKGFVDSLPAQFRDIDVLVNNAGLVKGTAKAPDIADEDIDVMFSTNVTGLINMTQAILPIYKKREDGGRGDIINIGSIAGREAYPGGSIYCATKAAIRSFTDAMRRELVATRIRVMEVDPGQVETEFSVVRFYGDKAKADAVYAGCEPLTPDDIAEVVVFNAARRENVVVADTLIFPNHQAAATVMHKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIQ5</accession>
    <name>A0A8H6FIQ5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156928.1">
        <property type="nucleotide sequence ID" value="XM_037298293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIQ5"/>
    <dbReference type="GeneID" id="59335801"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034039475" evidence="1">
        <location>
            <begin position="23"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="210" mass="24325" checksum="A808D8C0FC664FED" modified="2022-01-19" version="1">MPYLQRLLVGLMLFSPAIYALATPEQVARGKVKGFTGPADVELTGDYLTATFCYCEYPPRIEEYIGLTHYYQFEYYNTHLDTTFILNSTCPQDYVGYDFGCVPPARGQHVLAGYTMDEKCRTWHGEHDDKFCYQAWSSQGDDYIYFNDQKRGLGQMGSQGPVWKHKMEAEKVCEGMCRENMDAELFRDDDYLPSHQVVWTDLDDMCDHCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIU5</accession>
    <name>A0A8H6FIU5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007409</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156935.1">
        <property type="nucleotide sequence ID" value="XM_037298300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIU5"/>
    <dbReference type="GeneID" id="59335808"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="402" mass="44482" checksum="1FA5ADE68CCB706E" modified="2022-01-19" version="1">MARTLPWLKSGTTTNLQPARLKPAKRQRMLDPNSDSDSADNVNPRAVTTPKKQAAPAAERTPSTSPPPQPPTEEFMRPGLSADDIYIMVEDEFHAVAQTFTKHLHHAEYIRLRNAVRDRNAPTINNISRPVDSITTMREETRRKKEAEAREAKMKAAIESIKVPKGAIGPLDESDISDFEEDRQDDPWQGTQLQHFITTSPKKNLTGLTGLQGVISHTRAAAGYSKPEKRPSQPTKLFEPARPNASASKTIFAPAAGNATSDTDDDDLDAPSRLPPRPPTRIPSRPPTTAAKPTPLPTSRDPPHRQPKRRAPTPPPAKPFQRSFLDLTPRAPPASIPLSSKASRRPIPPDPSPPVRPEPLQEPRSSLSDVRQRLKVRREREERERKRSGSGIGVDDIPVFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK19</accession>
    <name>A0A8H6FK19_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Saccharopine dehydrogenase [NAD(+), L-lysine-forming]</fullName>
            <shortName evidence="14">SDH</shortName>
            <ecNumber evidence="5 14">1.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Lysine--2-oxoglutarate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_004322</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(+)-dependent cleavage of saccharopine to L-lysine and 2-oxoglutarate, the final step in the alpha-aminoadipate (AAA) pathway for lysin biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>L-saccharopine + NAD(+) + H2O = L-lysine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="EC" id="1.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the AlaDH/PNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.7" evidence="5 14"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157241.1">
        <property type="nucleotide sequence ID" value="XM_037295240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK19"/>
    <dbReference type="GeneID" id="59332730"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00034"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004754">
        <property type="term" value="F:saccharopine dehydrogenase (NAD+, L-lysine-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd12188">
        <property type="entry name" value="SDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000217">
        <property type="entry name" value="Saccharopine dehydrogenase [NAD(+), L-lysine-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000627">
        <property type="entry name" value="Saccharopine dehydrogenase [NAD(+), L-lysine-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027281">
        <property type="entry name" value="Lys1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF23">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE [NAD(+), L-LYSINE-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018250">
        <property type="entry name" value="Saccharopine_DH_Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 14" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="9 14 16" id="KW-0520">NAD</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="19">
        <location>
            <begin position="7"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding" evidence="18">
        <location>
            <begin position="180"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="204"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="319"/>
            <end position="322"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="206"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR018250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6229984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="386" mass="42745" checksum="B21EBD42B8E99DFA" modified="2022-01-19" version="1">MAPVTLHLRSETKPLEHRSALTPTVTRKLLDNGFKVNIERSPERIFDDKEFASVGATLVEEGSWPNAPEDHIIIGLKELEDKDPFPLKHTHVAFSHCYKNQGDWEKVLGRFPRGGGTLYDLEFLTDEKGRRVAAFGFHAGYAGAALALLDWAWQLEHGNKEALPGKGHYQCKSDLDTEVKEQVSKGVQRNKGKSPRVLVIGALGRCGRGALECCRQAGIPEQQLVKWDMDETAQPGPYNAIKEADAFINCIYLSEPLPPFISTDFLKQGTRNLSVVCDVSCDTTNPHNPIPFANQPTYFSKPTITLPDFLNPPLSYISIDHLPSLLPREASEAFSEALLSSLLQLPERDTAPVWVGAEKMFKEKVALLPQALRQRETPVANGAAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL43</accession>
    <name>A0A8H6FL43_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004856</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157769.1">
        <property type="nucleotide sequence ID" value="XM_037295768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL43"/>
    <dbReference type="GeneID" id="59333262"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="172" mass="18923" checksum="6BEB8A80A3082F0F" modified="2022-01-19" version="1">MCLGSQDPKDLLVNINKDPTEKIHPSRTQLTMSSLKTAKLKAIKASTPESYMSHIAWVWVKTQELLLKAESDALEAKMLRLQKKYSRLSARHPAVKPAAVALNKDWFIKEGEAKDEKLAGLMKAFKAAGGGVEDAGKVERLLKIGLEWSMAVKMQLVAVRDAVGQLKKLSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY92</accession>
    <name>A0A8H6KY92_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phenol 2-monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007164</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PheA/TfdB FAD monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156692.1">
        <property type="nucleotide sequence ID" value="XM_037298057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY92"/>
    <dbReference type="GeneID" id="59335564"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02979">
        <property type="entry name" value="PHOX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012941">
        <property type="entry name" value="Phe_hydrox_C_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038220">
        <property type="entry name" value="PHOX_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050641">
        <property type="entry name" value="RIFMO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004:SF7">
        <property type="entry name" value="P-HYDROXYBENZOATE-M-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07976">
        <property type="entry name" value="Phe_hydrox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="8"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Phenol hydroxylase-like C-terminal dimerisation" evidence="6">
        <location>
            <begin position="416"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="70696" checksum="D15FA6E5CF7B3A24" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYB5</accession>
    <name>A0A8H6KYB5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156721.1">
        <property type="nucleotide sequence ID" value="XM_037298086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYB5"/>
    <dbReference type="GeneID" id="59335593"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="29466" checksum="8B34C203328B1BA4" modified="2022-01-19" version="1">MLEDTTPENQAMRPSNAHWTTRPNIDAVPTGDKTTSEEWTTEEGKKEMVAEDRKVRSSTTATTVSSESKQTGISSNVHTSSCFPEEETDPTSSPSEMLDEPDRKCTESPIDSDRAVYGSNSVHSESSESSTEGNGSKKNTTAAVDLDKVEAMGGQFDDLCIGREAASFHNPENDMEARFINDKVQKANTTIEDIDLRPEVYFRDPGSSFQVSARPKEPSKLALDAKGDIAYSRKVRFAPDSLLDFDLEEGYNIEGPRMRYPSCCIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYM8</accession>
    <name>A0A8H6KYM8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156885.1">
        <property type="nucleotide sequence ID" value="XM_037298250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYM8"/>
    <dbReference type="GeneID" id="59335758"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="213" mass="23430" checksum="6199D83351278303" modified="2022-01-19" version="1">MSQIPVTEVCIIDLKAPYDLKDPSTPEGAIWASSISTLKKCPGFRDIYTSSVVEDRGKAHWDSKADHLSFYDTPHFPAFRAQLLSLCAVPPIPIHVLFHPAQPKTLQAPVTEVVMAYFPLAITTEQKEKWESIWQGFVTKVGGEVEGVRDVHGGWVVEGMEVEGLEGEMRAWMGTVGWEGWGAQKGKAGEGLVGVLDGAEGLVKREIYHVELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZS2</accession>
    <name>A0A8H6KZS2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157460.1">
        <property type="nucleotide sequence ID" value="XM_037295459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZS2"/>
    <dbReference type="GeneID" id="59332950"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019416">
        <property type="entry name" value="NCBP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16291">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16291:SF0">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10309">
        <property type="entry name" value="NCBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="498" mass="55449" checksum="5853D4251438E55D" modified="2022-01-19" version="1">MEVSYPSSALERNINMDQSTADMELDSMDLDIDLGSTDEFNEPSAVTNTISQISIPPSQVSDSELVGHKIHIRGLEDLTTSDIKAFSCEHYPFKAPLRVEWINDISANLVFEEEKTAMTALQHLTLPPNDNDFLPLSRLRAAKTLSTHPGSSLYVRIGATTDQKRPRAYEASRFYMMHPEHDPREQRRRGGSFQGNGDYRRKGYSGDEHRRRRRRDKEDGFDESIYDDGDPSSRRGSISSSANERSIEGKRNARLRGDSYRPARDERNGARSSRDRSASPNRRNHPTPPPIYRSRDPHPFPYENKGKELFPSTSRSDRNDKDLFSSKMIIADVSKGLNSTKSALPREAAPSGKDLSSNRTRAAEVKKELFPHKVNPSKHSRSDAFDAADETADLFANGMSVPFMDGPSSRKSPVDRLRSSGFQSQVDGGNIGTGFNIRGASREQNQGFSIRGGAAVNAIGTIKELFPGKAAGNAGKELFAEKVEGRGGRRNRAEDMFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L002</accession>
    <name>A0A8H6L002_9LECA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157700.1">
        <property type="nucleotide sequence ID" value="XM_037295699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L002"/>
    <dbReference type="GeneID" id="59333192"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034809060" evidence="1">
        <location>
            <begin position="27"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="869" mass="95499" checksum="3B84E6AA62FE0D1A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C4L9</accession>
    <name>A0A8H6C4L9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146935.1">
        <property type="nucleotide sequence ID" value="XM_037297734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C4L9"/>
    <dbReference type="GeneID" id="59335238"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="195" mass="21314" checksum="9E5C24D2AC50E5B4" modified="2022-01-19" version="1">MENPKAQGNVQEFHLPEWETTARHRGEKQVQIGKKSGVKERVFLVFDRVMPGHRKYFGFSRKIACIAIFVGLLILLAVILGLAIGLSKKSSNHQNLPLGSQTYTGDLTYYGPGLGACGITSSDTDHIVSISHFTFDAVSKGSNPNANPLCGHKLRAVRNGNSIDLTVVWAVNLLTWTSALGLSSNWLIRVLDALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6B6</accession>
    <name>A0A8H6C6B6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006532</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147140.1">
        <property type="nucleotide sequence ID" value="XM_037297430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6B6"/>
    <dbReference type="GeneID" id="59334933"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="24"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6217705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="363" mass="40356" checksum="BD3B7772EC14BE2C" modified="2022-01-19" version="1">MAEQFLHQLLRKENSVQSDESQQCHICLSEFGTLSSDTGVIECQIRLPCNHTLGSACIATWLKSNNTCPVCRHVLFPRPADDDDDDVDDDDDESQIVRSGRTSPVYIDTDDEEYDEHLSTRVARAARISELTFSYSAALHLNASITICSHGIAEKCGSLNSLPGRSEASIAAASVYMASHIGLQPKTLREISQLADNTEGTIQTVYRRIYAYFHTMPEGTWTQICRGLPWHSDDWPPLEHEFVDGETGEDEEYMEDIWSHFSTELHLQPTVVTIAKRMAIKLEAWPALASYEPISLAAICIYIASYMAGSPESHVEVARVAGDSLSFLLSAYEQVYADREQLLDSTMTTVDLPCPPSNGNPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Z9</accession>
    <name>A0A8H6C6Z9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_006104</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147580.1">
        <property type="nucleotide sequence ID" value="XM_037297006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Z9"/>
    <dbReference type="GeneID" id="59334508"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11063">
        <property type="entry name" value="CYP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002974">
        <property type="entry name" value="Cyt_P450_E_CYP52_ascomycetes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF1">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01239">
        <property type="entry name" value="EP450IICYP52"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034998471" description="Cytochrome P450" evidence="9">
        <location>
            <begin position="19"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6218145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="522" mass="58407" checksum="BBBFC1613482BBD5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C750</accession>
    <name>A0A8H6C750_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006181</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147655.1">
        <property type="nucleotide sequence ID" value="XM_037297081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C750"/>
    <dbReference type="GeneID" id="59334583"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034003475" description="Protein kinase domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="263"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="601"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="697"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="775"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="834"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="877" mass="97398" checksum="B14169B4914998BE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7E3</accession>
    <name>A0A8H6C7E3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147441.1">
        <property type="nucleotide sequence ID" value="XM_037297316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7E3"/>
    <dbReference type="GeneID" id="59334819"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="556" mass="62457" checksum="0EC7F3051DE39FB7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7V5</accession>
    <name>A0A8H6C7V5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147828.1">
        <property type="nucleotide sequence ID" value="XM_037296647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7V5"/>
    <dbReference type="GeneID" id="59334145"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031563">
        <property type="entry name" value="MOT1/MOT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31970:SF9">
        <property type="entry name" value="MOLYBDATE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16983">
        <property type="entry name" value="MFS_MOT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="424"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="51587" checksum="9EC309BE4B9D58A4" modified="2022-01-19" version="1">MPIVRHQLPVDNTNHEIMSSTDGPVSAPSRRSQLLTTLRESSRKAQADFFQTQRYNIETFYNQPLAEISGSFGDIGTLLPILIALTQIGAISLSSTLVFSGLANILTGLAFGVPLPVQPMKAIAAVALAQGFSKSEIASAGIFVAAAIGFLSITGLIHWFTERIPIPVIKGIQVGTGLSLIISAGNLYPHFDRFGTNPLVLILAFLGLLASSTFRRVPYALLILLVGIISVISTDWLYPGWGEDFRPDFSTWHPHTFVPSPREFGKGTLEAGIGQLPLTTLNSVIAVAFLAQDILPNVRTPSTTSLGLSVMAINLVGCWFGAMPVCHGSGGLAAQYRFGARSGASIIFLGLLKLLLGLFASKVALAIFNGFPNVLLCVLVIAAGLELVGVGESLNTARSRDLSNLGEDGGKGGELTEEERKRRWAVMFMTVAGILAFKNDAVGFLAGVLCHWSFKAQDRLEERRGGEGRIRLGEGREGERNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C876</accession>
    <name>A0A8H6C876_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148132.1">
        <property type="nucleotide sequence ID" value="XM_037296951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C876"/>
    <dbReference type="GeneID" id="59334453"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="493" mass="56275" checksum="CE380AB6812983CB" modified="2022-01-19" version="1">MNIKLVAMRCSLVDLSDELHLNIIQELLKHDDFLHEESLKPDPKRYNQEWDDDFQYHRDLMSWSSTSLYFRNLLAPYIFKSIKLRNDVKSGASVDAVLKSPHGALVKGIYFLGTIPPDIDDLDDDEDDDPLAEGSYKESDDAEGEERPIIITFPPVVNDLLSDLHQFPNLESLSIGFTYPYDDPFDEYYETEGIMVNENPTAARARKALMTTTYETLLRSKPPQLRAVEIRKFVWTFTEPYESQGFQKFLSHVEHFRLSVRGGNNGACWCVNTCDGYLESVARFDELFFDHLASATSLTLRATDEGPIGLEGMRHARLALTKEQMPLLQSLHLEHIFICQELVDFVLGHTDTLERLTLHDCHSSVNGLQDNDGFYWAHFFETLHGADLKKLSHLEICPDDAPLTFEPDSLQKEPVCPGDVRQIRRVPSDVAKRRVFGYAMLDDKYGECNDDYEENRAAFERGEDQAAFDRLMSNVNANAANRGGKITDWQVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8K7</accession>
    <name>A0A8H6C8K7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein 14 C-terminal Fig4-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006000</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAC14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148084.1">
        <property type="nucleotide sequence ID" value="XM_037296903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8K7"/>
    <dbReference type="GeneID" id="59334405"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026825">
        <property type="entry name" value="Vac14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021841">
        <property type="entry name" value="VAC14_Fig4p-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023:SF0">
        <property type="entry name" value="PROTEIN VAC14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023">
        <property type="entry name" value="TAX1 BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12755">
        <property type="entry name" value="Vac14_Fab1_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11916">
        <property type="entry name" value="Vac14_Fig4_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="87"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein 14 C-terminal Fig4-binding" evidence="7">
        <location>
            <begin position="610"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="285"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="430"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="842"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="844"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="907"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="918" mass="101695" checksum="4381017739A6B248" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8R0</accession>
    <name>A0A8H6C8R0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DJ-1/PfpI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148508.1">
        <property type="nucleotide sequence ID" value="XM_037296526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8R0"/>
    <dbReference type="GeneID" id="59334023"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052158">
        <property type="entry name" value="INH-QAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43130">
        <property type="entry name" value="ARAC-FAMILY TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43130:SF3">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR RV1931C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="1">
        <location>
            <begin position="16"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="293" mass="31720" checksum="1B81F5E50294696F" modified="2022-01-19" version="1">MSAIAESSDGPIEVIQVLIVLHPSFGAQELCGPLEVLSNAYHKLNDPGSKAFECTIAAASPGVTSSSGLTVKADINFEDAHEDLKEYDVLIVPGGVGVDDVLKKEAEPIKLIQDFAKLQQTDPSKERTLMSIGTGSLILAHAGLLQGLAATTHPDYYTKMEIICKEAARRGDLEQTDVMEENYVVNNARFDLGEKLEENPFVLNKRPDGRRKSIARKGSNAWKESVKRRESNARRASLRLGGLRVITSGGVTSGLDASLYLVAAMVSHESAQEVARVLQYSWNKGITVDGIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Z3</accession>
    <name>A0A8H6C8Z3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005599</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148490.1">
        <property type="nucleotide sequence ID" value="XM_037296508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8Z3"/>
    <dbReference type="GeneID" id="59334005"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF21">
        <property type="entry name" value="PYRIDOXAL PHOSPHATE (PLP)-DEPENDENT ASPARTATE AMINOTRANSFERASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="1">
        <location>
            <begin position="22"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="426" mass="46175" checksum="7FF9F9BA152BD398" modified="2022-01-19" version="1">MASFSPTTARAKFPALQQSHQIYFDNAGGSQALGTVASSVKDYLEETNVQLGASYDVAKQSTESYGRGLEAAAGFIGAASDEIVIGPSTTQLFSNLSLILNLPPGSEIIISSLDHEANISPWVRLAKLYNHTIKWWPAAPASPTHASPSPILTSENLKPLLSEKTALVTCTHASNVLGTIHDVKEIAQTVHMIPGAKLCVDGVALAPHREVDVKALGVDFYAFSWYKVYGPHIAILYASRDAQRNLGSLGHYFHTGTNIETKIGLASASYELVAALPSIVSYFGPDKSATWAAIAAHEEKLQGILLRYLRSRDDVTIHGEKSADKELRVPVVSFTVNGRSSKDIVDGIESKSNFGCRWGHFYSKRMVDDLLGLGGCDGVVRVSMVHYNTEEEIERIDRGKVRTLKDLPVLVRPLVLSFIDASILAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9A5</accession>
    <name>A0A8H6C9A5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">DNA replication regulator SLD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005168</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Has a role in the initiation of DNA replication. Required at S-phase checkpoint.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SLD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148778.1">
        <property type="nucleotide sequence ID" value="XM_037296080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9A5"/>
    <dbReference type="GeneID" id="59333574"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22289">
        <property type="entry name" value="RecQL4_SLD2_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1460:FF:000001">
        <property type="entry name" value="DNA replication regulator Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021110">
        <property type="entry name" value="DNA_rep_checkpnt_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040203">
        <property type="entry name" value="Sld2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124:SF1">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11719">
        <property type="entry name" value="Drc1-Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="35"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="317"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="466"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="190"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="489" mass="54652" checksum="19192B903FBDD3B3" modified="2022-01-19" version="1">MTMDGSNCATAAEIDTLAKSLRLQLKEWEQSFAVAHQGRKAGREDIKQHPDIAHKYKQYDRLRRSSSKQATPHNTTPSRKRPTTNPHPVPSARSPYKRPKHAHYTHPLTSRSSDPPSPTHVSPRTHRTSIGPTPQKDGQVLGLFDQLSTSSNLQTPSKRKALRSVGGNMLATPSKRPQPKDRKHTDRMHQNSPISGTKSELLNDLFTPSTRRTTDCSTPASRGGLSKLRSDDTPAFLRRDSQRADVEKENVLRDETGVSWSPVAIRRLPKAAGRGLSALVRGLRQMEDEGLDEELDMLREMESEITPRNHLDQPKVFVNDSQRPDMPLGPDGGLESDEDGVEYANEGKGRNGKPLKVWKKKGQKRTTRRVLMKPNTAKWKPEPAWKVGQANRDEEDGIAETQTVSADRSIDWKERGCDSDEYEDIEGSQGATRAENGKRDAFDKLGKKEGLAKKVKKKISATAHANFRALKIKNKQSKGKKGGKFGRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9B4</accession>
    <name>A0A8H6C9B4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148717.1">
        <property type="nucleotide sequence ID" value="XM_037296019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9B4"/>
    <dbReference type="GeneID" id="59333513"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="727"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="853"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="581"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="591"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="859"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="893"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="961" mass="104749" checksum="9BEB2EB0039F27C4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA40</accession>
    <name>A0A8H6CA40_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003532</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149142.1">
        <property type="nucleotide sequence ID" value="XM_037294454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA40"/>
    <dbReference type="GeneID" id="59331943"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037460">
        <property type="entry name" value="SEST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37981">
        <property type="entry name" value="LIPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37981:SF1">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="35155" checksum="DE67418994A6FE12" modified="2022-01-19" version="1">MGLQTGTNPTIADIATVSIGGNDIGFYNILTACVLWVGGNWAGDCDTEVAKANEILASPDLSTNITSVLKEIIDKNGNDAFKVYMTGYVTFFNEATPLCESSSFRIWNPHYDNTHKEKGQPWLTTALRAQLNGVVVALNTILSHITSSVNAYYGNTQRIVFVDPNSAYAGHRWCEDGVYEPDNGRLNTWLFLSSTPDNSLPDDPLGALESYQQEQSELVAGPDFPLPDPTTCRETLTQFNSLVRSDWYENMLCDISIATSSASSFEYQVIQKEAMQIQQGNLTKLRIPWYVPTRLAKTFHPKTLGQLAFANEIMAVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAG1</accession>
    <name>A0A8H6CAG1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="2">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_003707</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149317.1">
        <property type="nucleotide sequence ID" value="XM_037294629.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332118"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140684">
        <property type="term" value="F:histone H3K9me2/H3K9me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15571">
        <property type="entry name" value="ePHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000024">
        <property type="entry name" value="Putative jumonji family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055500">
        <property type="entry name" value="DUF7072"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF7">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23258">
        <property type="entry name" value="DUF7072"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="8">
        <location>
            <begin position="81"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="9">
        <location>
            <begin position="350"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="597"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="161"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="531"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="821"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1015"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1222"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1323"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="537"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1015"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1065"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1115"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1128"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1222"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1242"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1268"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1288"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1349"/>
            <end position="1379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6219882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1461" mass="164632" checksum="D46FF8E748D950CD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAQ5</accession>
    <name>A0A8H6CAQ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor C subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003780</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149390.1">
        <property type="nucleotide sequence ID" value="XM_037294702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAQ5"/>
    <dbReference type="GeneID" id="59332191"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000028">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000004">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000129">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF9">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="77"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="395" mass="43214" checksum="A9D090D10308C51C" modified="2022-01-19" version="1">MSDYEDEMDVDDAPVKESVMFSSDNTNAKGKRSAANLPVEAEDSLPWVEKYRPDTLDDVSGHQDILATINKFVDTNRLPHLLLYGPPGTGKTSTILALARRIYGNRNMRQMVLELNASDDRGIDVVREQIKTFASTKQIFSAAPKAGDTPSSGAYKLIVLDEADAMTSVAQMALRRIMEKYTANTRFCVIANYTHKLSPALLSRCTRFRFSPLKEPDIRQLVDIVINKEDVKIQPEAVDSLVRLSKGDMRRALNVLQACHASSTPLYIKGQPPPKNPKREMITNATIYDCIAAPHPSDIELIRDTLMESSDVTSCLNTVNMLKANKGLALADILTALGEELAKLEVPPQTRVAWLSGLAEVEYRLSGGGSEVVQTGGLVGVVRGGVDLMEHGGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB77</accession>
    <name>A0A8H6CB77_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149649.1">
        <property type="nucleotide sequence ID" value="XM_037294268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB77"/>
    <dbReference type="SMR" id="A0A8H6CB77"/>
    <dbReference type="GeneID" id="59331756"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="212" mass="24003" checksum="D35A2F726078EDD4" modified="2022-01-19" version="1">MNSYAERLASFTSWPHVCPSASTVARAGFRREYDPTFPSPDLTVCSLCGLDPCDWDSDGTPTLFDFHATRLPECPFVRATQQKKKHRNQKTRQQYTRRSLQAVTQQEEEPLQQANAENTAEKSSDNKATISKNTAQLSTIPRHMLSTVPRQITTQQAENDTTIWPGISACLNNITLIWYITGMTSQEEDLLRRSQPIGSLQDGVRTMHIWTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBE1</accession>
    <name>A0A8H6CBE1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003451</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149754.1">
        <property type="nucleotide sequence ID" value="XM_037294373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBE1"/>
    <dbReference type="GeneID" id="59331862"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="494" mass="54408" checksum="7BE5FBB8CC2FF4B5" modified="2022-01-19" version="1">MLKGSNLSLNLENAGWRSSGGPFDDGMNIKKAKEVEDDTFVLDWAARSAPVTYPRCARGEAPKRTGRPDLYLSKPLDFNIVYPFIRTFVGDFFTRKQSRLYKLSQLAMTAILQTFACFFSLSIAFALPSNLVRLPLAKRDVESIPSEVNTTIRNPLTAQPVACLDTRRLPTLVPSDCGYVLNFIILQEPNVFAKRVFIRNSYQTEARGYARSRWQYGSCEVSVYGPPGARQLLTFLEVAFTADNIVRECVTGFVDPIGGVSLVGDASEAFYVYLRGHVEPESISAKNSTISQRPAVSVSRRTMRSQKDSENGAGSQRVETRDPSMGASRVSNLPTVTSNFTLSPAALPRYPVHCFNPFIIHLQPAAATDCGVIINQVILRLFDPTSQLNFGFTDAADVDLSKPEYQKWQYGQCMISVKNNNVAQVDTFRLLDVATTARRISTQCLVNTQDKIGGVASIGTDGRGFYVYVGGRLASSPALSDLTLLRESTGAESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBR5</accession>
    <name>A0A8H6CBR5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002912</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149915.1">
        <property type="nucleotide sequence ID" value="XM_037293837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBR5"/>
    <dbReference type="GeneID" id="59331324"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="475" mass="54028" checksum="07DCD670CEC485DF" modified="2022-01-19" version="1">MSIYSVRDIKGEEALISTSTRVSDLTNSRLSGHLNVREVSSKATFPFLDLPAELRNKIYGYLLSTKYTKVELTYHLQGPRDGFPSYQPSHYYRFQTGILGKNRQAYFEAADIFYRKNLFVSLTHSRFGFDSKMAGVVVVATDHHASNFKHLSMRVHISPHWYSDERSHTPFDQFIIAVDDLPSFCNILPGLNIHGLRHWKRGIWISTNVCVQINVQRFADVERSHTKPETDPSSVKKLLEPFRRLYGMRVRVGGHVTPSYKESIEQCAARQPPTAADLVRMISKRREEGTQSMQSSNIATAVTTFESALDLLRSGHMRHTIHPVTVATPETPDKQVTTAMQTLEINLRCLLASSYLELGEHAKSYRCAQDLRLTKLFDRYRTFNPPEGPDWARIMFIQAMAGKALGQPVQALMDLDLGLTFSPDDEAMKEERKSLCVFVRKKMNSDLQMNGARALDRRTTRLKKRQPSAVAQSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDP4</accession>
    <name>A0A8H6CDP4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001668</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151135.1">
        <property type="nucleotide sequence ID" value="XM_037292596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDP4"/>
    <dbReference type="GeneID" id="59330082"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14710">
        <property type="entry name" value="bZIP_HAC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044280">
        <property type="entry name" value="Hac1/HY5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714:SF6">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0834">Unfolded protein response</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="92"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="357"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="361"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="392" mass="43117" checksum="EDD58433E21C4E14" modified="2022-01-19" version="1">MAEVLSPAMAFGTNDFYNPQATISLSDTSLQNNLMTPEPMSPESSYNVKEESVEGDESKKPTKKRKSWGQELPTPKTNLPPRKRAKTEDEKEQRRIERVLRNRQAAQSSRERKRQEVEKLEGEKSFIEQQNESLKERLMTVEHEKFLLTQQVAKLAAQMEAIKRGCSATPRVDSPPLEPDLLTREQFIKQELLDDYPYNLPTPQHSHGAPSTSYSSPSTATYSESSTPATMGLDLDALTATPDMTQHPAAMFTSNIDSAYDSPRDPIIFGQSSLSDVDFGSLFATDASVEDDLHFFKDGFAASSIDDDNANGFTFDSMVDLDACQSTTAFTNKIDDTNTYNNEDLARQSDFYSGGYSLDGSNSSSQIAATSSAQQPFPGAPSKGCDGLGTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE39</accession>
    <name>A0A8H6CE39_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001562</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151031.1">
        <property type="nucleotide sequence ID" value="XM_037292492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE39"/>
    <dbReference type="GeneID" id="59329978"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03445">
        <property type="entry name" value="Thioesterase_II_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049450">
        <property type="entry name" value="ACOT8-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF35">
        <property type="entry name" value="ACYL-COA THIOESTERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20789">
        <property type="entry name" value="4HBT_3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like N-terminal HotDog" evidence="1">
        <location>
            <begin position="41"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA thioesterase-like C-terminal" evidence="2">
        <location>
            <begin position="152"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="263" mass="28641" checksum="394EEBA8D86DFB8F" modified="2022-01-19" version="1">MGNLGTEGESTGSDFIEELEVETSSEGYYVNVQPMWSFNDWGIVPGALLMPQCVAASYRTVPLGFHTHSVQTHFLGNGTVTKRATYRVENLNDGKTFATSKVKVEQDSKTIVLTTVGFAKRASVAGRGVAVVEHAVPVVMRFDTHILRKFILTSPGPSPAAESKLARHWLRADGPVSTENGNMQNVLGLVALSDIFLLDSAPRIYGLGFDLTTEKSPSERLARDLKAMTTINHSIHIVNVDAARVDEWVYVECKLHGRAVGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEC2</accession>
    <name>A0A8H6CEC2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151114.1">
        <property type="nucleotide sequence ID" value="XM_037292575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEC2"/>
    <dbReference type="GeneID" id="59330061"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="116" mass="12573" checksum="78F6191859FC8F4F" modified="2022-01-19" version="1">MTLPVDSTSILYLSEAPNRGGNTTPSVNPKQASGILKNVVDRQAEEETKKISLKGKRHAWLVDGIQDGQVRGHVQDGVVDADAEGNLFREWLSDSLASSPVSGWVVVMQISVDLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEN4</accession>
    <name>A0A8H6CEN4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151527.1">
        <property type="nucleotide sequence ID" value="XM_037292108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEN4"/>
    <dbReference type="GeneID" id="59329594"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="101" mass="11212" checksum="16B687CF19DCA139" modified="2022-01-19" version="1">MADNEPSGPFHAGPGTAHEIGVMAGFIAAFIIITVVYLIIWKVYNKRSEAGEMQRRHILAEKTNPRNTRVMEEKAGNRRCAGLLKSMEKIPRGCGAETEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CER1</accession>
    <name>A0A8H6CER1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001175</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151524.1">
        <property type="nucleotide sequence ID" value="XM_037292105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CER1"/>
    <dbReference type="GeneID" id="59329591"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00413">
        <property type="entry name" value="Glyco_hydrolase_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38121">
        <property type="entry name" value="GH16 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38121:SF5">
        <property type="entry name" value="GH16 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034790023" description="GH16 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="583"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="4">
        <location>
            <begin position="54"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="603" mass="63236" checksum="5AB92902CE5DCA39" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEZ0</accession>
    <name>A0A8H6CEZ0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151692.1">
        <property type="nucleotide sequence ID" value="XM_037292273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEZ0"/>
    <dbReference type="GeneID" id="59329759"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="128" mass="14709" checksum="30F431C29AB3964B" modified="2022-01-19" version="1">MTQLMKASPGIRLSLGDVYQEPPTRMTRHNTLGGRAIDAIRQDLEFGNGEYDANYKRPVFVKVSKATATDSSFRLFGDELKKRQKIYNAVLFVVSHRAIFKYRARKTIKAAYEQRFYVSEKQKANLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFM4</accession>
    <name>A0A8H6CFM4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000659</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151958.1">
        <property type="nucleotide sequence ID" value="XM_037291596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFM4"/>
    <dbReference type="GeneID" id="59329077"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000435">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF9">
        <property type="entry name" value="TAH-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="49"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="688"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="797" mass="87907" checksum="AD195D3B160A1031" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFQ3</accession>
    <name>A0A8H6CFQ3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152066.1">
        <property type="nucleotide sequence ID" value="XM_037291704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFQ3"/>
    <dbReference type="GeneID" id="59329185"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050471">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433:SF5">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="201" mass="22443" checksum="C5B31FA063BEF0E1" modified="2022-01-19" version="1">MAKDTLELIDHVGWTSQRQLHVIGISLGGMIGQELVIISFQFNKESSLLSPDVGFDDVQLDSIKARTFSQPWLNETDAEGHFPTNGDRWAAQELAKRQNVEGFTKKGFILQAIAVNWHHKSAAQLKQLGDQVGRNRILVIHGTEDRMITQPHGDLLVKELGGEENGVTRCVAEGRGHGLPMEWRRELTKVIAIFVEKTGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFU1</accession>
    <name>A0A8H6CFU1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152033.1">
        <property type="nucleotide sequence ID" value="XM_037291671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFU1"/>
    <dbReference type="GeneID" id="59329152"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="478" mass="52147" checksum="D80C16A36FC66235" modified="2022-01-19" version="1">MLWPRSVLVEEETPEIGFILLEADVVEPGSGVEELFVLGAIALLKLGSALIDDLLVPTLILGVEGEKVLDTDVMFIPGPGFARVLENNKVLGVSPVVEAVLELRPVLELDELDPFFVLAKEPEELDIKFMLEALLDARLALEFDGPNPVLVLALVLEGDRVVDIGYAPEVLLNATVALEADVLDPMFRLDGATDELMHQGSSRLQRLAEALLDVDMMDPGSMLEAMLEDNMLDIWSVLVEVPAIESPVLIKVLVAWPKLEEGVLEIELTLDPRSETEELEFEEPVPEEALDAGTELEEVMESAVELDSRFTLEVELLELVAALDPGDAALDPGPVLEELLGVALEGALELVLGEVTEEVPEEVFDSGSALEVVLGVVLGAASKKRPLIVWVTAAELALNPGFVVDDELDPGLVQDDELDKPVGKLDDEVEEKLEKAVIPRSMWEDVPDIDNEEVINGELPIEEKLLVDKRLLLDTKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG88</accession>
    <name>A0A8H6CG88_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152017.1">
        <property type="nucleotide sequence ID" value="XM_037291655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG88"/>
    <dbReference type="GeneID" id="59329136"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034068966" evidence="2">
        <location>
            <begin position="21"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="530" mass="58038" checksum="397760CB86CADC48" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGC3</accession>
    <name>A0A8H6CGC3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152077.1">
        <property type="nucleotide sequence ID" value="XM_037291715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGC3"/>
    <dbReference type="GeneID" id="59329197"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="156" mass="17121" checksum="DBF47C9B9A0C6AC8" modified="2022-01-19" version="1">MGTYSGGPVGDLDADYVQTATIQNTRPFDPDLPGYQYNFLPVDDGSWRRVQIDVDWLNTLTPSPRAQHVRLDQPRRPSYRHGHGQQSGMPGASGAVDVFKNASAGIERYRTMSTQVRVMSLPTSGATASQEDVKILFGDKTPTAGYRASEIDKPYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGE0</accession>
    <name>A0A8H6CGE0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dehydroquinate synthase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001089</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152383.1">
        <property type="nucleotide sequence ID" value="XM_037292021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGE0"/>
    <dbReference type="GeneID" id="59329507"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08192">
        <property type="entry name" value="MAR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF97">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE IRON-TYPE_GLYCEROL DEHYDROGENASE GLDA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00060">
        <property type="entry name" value="ADH_IRON_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="2">
        <location>
            <begin position="19"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="185"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="39972" checksum="B19440E2DB0252A4" modified="2022-01-19" version="1">MTRSNPPSGLWKPTHLERLYYGPSSVHDNLVSCLPSSSSKAFIITGSSLATKTPLIQQVEKLLTSKHHAGTFSNIKQHAPVAQLDEATDAVQKDSSIDTIISIGGGSPIDSAKAISYRTHEKSNKYLYHIAIPTTLSAAECTMGAGYTAADGKKTGVGGPELVPNVIIYDAKFALETPQRLWLSTAIRSLDHAVELLYHPKAAEAPAKSLVLSAIEGLFTYLPKSKAEPKNEDYITRLQLASFNSLFPIGLDMGGGLGLSHAMGYALGSPYGIPHGITSCISLAGVVRLKAENPEDAAQVARALPYIGQSKSGDDKKDALKVADAIEKLVKDLGLESRLKDYNVGSDQVSVVAKTATKSESGPLYDGVAKIVESKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGN1</accession>
    <name>A0A8H6CGN1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152447.1">
        <property type="nucleotide sequence ID" value="XM_037291012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGN1"/>
    <dbReference type="GeneID" id="59328491"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="236" mass="27013" checksum="4ACA076B81CC5056" modified="2022-01-19" version="1">MVLYNDIEFLLMIEIDSDWIKETKRMCRKDIKRYAKFLELKERPKPLGKLALPVKLDVYSAVIQRGKHDVASQQAATFRASTGQTLGETKQATTSNDRPGQVERPKLSLRSLPYVYTDPEPLLCANEDKLQHDSMRREHSLILKAALPLTPATSSELPIRTTRDTSFDLPIRNHSGRLLELLAMQEQDRVPTWRTWVANRPGDLHVAEVKDRSQAREKQDAALPRWQCTHPIGPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGX8</accession>
    <name>A0A8H6CGX8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152410.1">
        <property type="nucleotide sequence ID" value="XM_037290975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGX8"/>
    <dbReference type="GeneID" id="59328454"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="227" mass="25424" checksum="D43BD1F3205DABBE" modified="2022-01-19" version="1">MFTPTSRQWLSVLRLTPIILGSILLFACTDLGLAIAYAKQLFNAPLDESKWDYTRDRRWSTAWFLLYSGFFPFLSVTHITVDAALFFFAQLNPICTLTFSILLSLGWIIQLSFWCHCDYGPDSNTCYQFYIMGDQHSDMNSSLSGVSGGITGAKVIFGMIVLVLYIAYLTLAATAVENMRRTGHVRIDDQEEQGLEDGYSIENRHLASHSVEHLRSLGSLLRGGTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHQ2</accession>
    <name>A0A8H6CHQ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152914.1">
        <property type="nucleotide sequence ID" value="XM_037291479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHQ2"/>
    <dbReference type="GeneID" id="59328959"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="29147" checksum="FDF02341893837EA" modified="2022-01-19" version="1">MLTQLCTGPSEVTVFEPEVLGAVDGPGSHCTKAVWYDALQPESLVNTTRDNGIHTQRRRMWNNAFTPQASTASDEQKALSGYEDQIVFYARRFENEVARSIGSPVDASSLIYKFTFDLMGEIAFSRNAGQDRLRDQQWHAAMTALQKGMALLGPLSPVPWLLHIGLSLPFIPIVRDWNSMVAFCKSCIDDRIKVDNLSSGTICDPRMALEMTKCVPVDQLEETDHDISSWLIDSSQAKTLSEEERVKWLYGDAITMVIAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIH9</accession>
    <name>A0A8H6CIH9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.131</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_010564</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:29523"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="EC" id="2.4.1.131"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 14">
            <dbReference type="Rhea" id="RHEA:29524"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.131" evidence="4 14"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153050.1">
        <property type="nucleotide sequence ID" value="XM_037301416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIH9"/>
    <dbReference type="GeneID" id="59338954"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004377">
        <property type="term" value="F:GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03806">
        <property type="entry name" value="GT4_ALG11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000168">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Alg11), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038013">
        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031814">
        <property type="entry name" value="ALG11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919:SF1">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15924">
        <property type="entry name" value="ALG11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="ALG11 mannosyltransferase N-terminal" evidence="17">
        <location>
            <begin position="96"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="16">
        <location>
            <begin position="339"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="47"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF15924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6223990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="540" mass="60196" checksum="708BA54E576BB7E3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIN9</accession>
    <name>A0A8H6CIN9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010503</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152989.1">
        <property type="nucleotide sequence ID" value="XM_037301355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIN9"/>
    <dbReference type="GeneID" id="59338893"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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            <begin position="555"/>
            <end position="640"/>
        </location>
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        <location>
            <begin position="648"/>
            <end position="675"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1082" mass="118848" checksum="0C6517D97CAAFA0C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIU7</accession>
    <name>A0A8H6CIU7_9LECA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">HO133_010729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
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        </location>
    </feature>
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        <location>
            <begin position="525"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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            <begin position="182"/>
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        </location>
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        </location>
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        </location>
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        </location>
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        <location>
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        </location>
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        <location>
            <begin position="820"/>
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        </location>
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        <location>
            <begin position="1074"/>
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        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6224155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1083" mass="119449" checksum="53BBC325E6DFAF7F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJG6</accession>
    <name>A0A8H6CJG6_9LECA</name>
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        <recommendedName>
            <fullName evidence="1">Ubiquitin-like modifier HUB1</fullName>
        </recommendedName>
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    </gene>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Lecanorales</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153545.1">
        <property type="nucleotide sequence ID" value="XM_037301911.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hub1/Ubl5"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13042">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
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        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
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        <location>
            <begin position="53"/>
            <end position="124"/>
        </location>
    </feature>
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        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="235" mass="27011" checksum="6C02C9044EF91473" modified="2022-01-19" version="1">MALSNHRQDRPRSRSPPRKPAYSPSPPRRDRPRSRSPRRRHDDRPRPSGGGFRWKEKPRYDDDEMDGRGERRLERGYREQERERPRPRSPDRRDGRGRERDGDVERKFGKGRDVEDKFGGRHDGAANSGSRDADKPQEKPKKERKPAPAPSGEPMIIVNVNDRLGTKASIPCMASDPIRLFKAVVAAQIGRQPHEIMLKRQGERPFKDQLTLEDYGVSNGVQLDLYVHICLLGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJK0</accession>
    <name>A0A8H6CJK0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010973</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153456.1">
        <property type="nucleotide sequence ID" value="XM_037301822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJK0"/>
    <dbReference type="GeneID" id="59339363"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="165" mass="16925" checksum="56309E570C65C1AC" modified="2022-01-19" version="1">MLDAQQPPTPLVPPPSYYGPPVLDTDVASCKSTDTTTCALGVSASTSAGYTITMGGGLTLDLPKIGSIAFTPSVSYSYTTTEGTSDGVNCPEGGYTCGMTSVTPWQTITDNQHSCGGGGWPACKPCGDDAYSIRVPAQTAEISWAACIASDSLNQKTGPNLEKCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJQ4</accession>
    <name>A0A8H6CJQ4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153655.1">
        <property type="nucleotide sequence ID" value="XM_037300838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJQ4"/>
    <dbReference type="GeneID" id="59338374"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="515" mass="56145" checksum="FE826CBB242F4271" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJZ8</accession>
    <name>A0A8H6CJZ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153805.1">
        <property type="nucleotide sequence ID" value="XM_037300988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJZ8"/>
    <dbReference type="GeneID" id="59338524"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00048">
        <property type="entry name" value="DSRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42030">
        <property type="entry name" value="DRBM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42030:SF1">
        <property type="entry name" value="DRBM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="14690" checksum="6C8D16F0A0D302D6" modified="2022-01-19" version="1">MYQYYSPSFIREKPSSASTLYARPTMSTASSSAGSTGSGVAQRSSTWQDRLQAHCSQRHLSSPEYKVFSDRRGKRTAWSCHITVSGLTIPARYWYDGQYVNTAKEDAAEAALQKLLGRGNGVMGSVGQGRVVGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK48</accession>
    <name>A0A8H6CK48_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010301</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153972.1">
        <property type="nucleotide sequence ID" value="XM_037301155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK48"/>
    <dbReference type="GeneID" id="59338692"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="363"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="74"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="510" mass="57126" checksum="0F41FCD7452848C0" modified="2022-01-19" version="1">MYNSLGPETNTRDTQKCSVCRHRRKKCSPHDRDWENSQQRCHFCETHALPCGPNVRYSEDPAVIRRREVVFENANAGRSSQAGERHSEGPTARSALNFPAPATRHRRGRNVGPPDAGSAEDGREPFGTESIADLEEKAFSRFDDFHALGTTLVYVTQPSWVYCPSHRAQQPYHDLSIEEKLHELFINIYDIAVEARDRNESHLARWILFDLVFALDQYRLFDTRKRNILLKMARMFQELRHPWHYAHVLLKIAGMYKGSALPTQEGRLLAESSHLLAESFPTSSMSIRRVLVDRWNETVGGIHVDSNLNVPPLHAAVQHRNPSIILALLSNPNDCSSTPLAPPLTMPSRVGQVRADIEERDLNSRTALFAAVANGDESCCSALLYHGADANIRDDHGHTALEVAVRGSYLNIVDLLIYYKALVNPDITGCSSLPLHAAIESSNLSSNLQLTIIHNLLKSGAEAYLRRHADNKHAIDLAVDRGEHELAESIRQKVPSLSQSHTAFMGQINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKQ2</accession>
    <name>A0A8H6CKQ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010359</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154029.1">
        <property type="nucleotide sequence ID" value="XM_037301212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKQ2"/>
    <dbReference type="GeneID" id="59338750"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="282"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="478"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="904"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="482"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="562"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="609"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="939" mass="98175" checksum="DE3189EE2F60CEFC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLA0</accession>
    <name>A0A8H6CLA0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010378</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154048.1">
        <property type="nucleotide sequence ID" value="XM_037301231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLA0"/>
    <dbReference type="GeneID" id="59338769"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097:SF9">
        <property type="entry name" value="EPIMERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G07320)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="26655" checksum="D5D61E34D3102E3D" modified="2022-01-19" version="1">MKLIVAGASGFVATEIIRQSLSLPKITSVMAIARRPVSTPSNLGQGADASKLHSVVLDNYGNYPDDAIRQLAGADACIWTVAITPSKSRGLDFDEVRRVCQEYTLVGLRAIFKAHSEGGSRGAPPLRFLYMSGTATERDQNKTPSWMPEYCLMRGETETQVLAFAAEHKGAVEACVAKPGLITARGQYLKTIFAMVMKYVMSLPSVDVAEISAAMLHEVIHGFEKEPLENNDLVRIGRQALKIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLC9</accession>
    <name>A0A8H6CLC9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase family 8 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154317.1">
        <property type="nucleotide sequence ID" value="XM_037300469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLC9"/>
    <dbReference type="GeneID" id="59338003"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="48371" checksum="183455851CD4932D" modified="2022-01-19" version="1">MGLPPAIEFAKADGLNSAQPYQKRPVNKRPVHHTSSRHAPHKRPNLLFIFTTLLFLSGYILQQQTVRSLQLALHPPPTPAIPVTDPVLQKHFGQPPGYGGGGFFYDKFLAKNRPRGGWAKVAYVQLVRRHVEVCGAVMGMREVERGESMAQRIILYPREWDGEVGGGGEGGDGDGDGKEEERRRRETSGRLLRKAAREYKVMLQAVGSIPLAEGSEAVTEEERYPLTNLLSLIHYNRILFLRPSGLILDSTPLDLLFTLPMEQPLLGLSAPEDGSMQPAILLFEPSKETYRDVISSLPEGAYPDGEFLQRVTTTPAPADGESPMTLLAQTSALGDVDAQFNATRFLDTTAYIRLSDPDLPGPEYDIPRQDYVRAMPGSKEARQAWEGVYERFRDARMGVCGLDLERVERHRSEMDDVVARENVETHGKKASEIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLN8</accession>
    <name>A0A8H6CLN8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009657</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154366.1">
        <property type="nucleotide sequence ID" value="XM_037300518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLN8"/>
    <dbReference type="GeneID" id="59338052"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="592" mass="67053" checksum="2D48757ED996234D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLT3</accession>
    <name>A0A8H6CLT3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA 3'-terminal phosphate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154426.1">
        <property type="nucleotide sequence ID" value="XM_037300578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLT3"/>
    <dbReference type="GeneID" id="59338113"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="427" mass="46858" checksum="476A873B16BBBC65" modified="2022-01-19" version="1">MSGQGYPTSSTPIHLDGTTLEGGGQLLRLALSLSSLTQIPIYVTDIRGKRPGKVSGLKASHLAGVKWLSNATAATTEGMEVKSRQLVFRPSSIRAETTVNKGQNEHVTRTEGEKEGVWEDIFDNGQLVRRQSHIPMSSPGSILLVLQAILPYLLMSNCNNVEGQKTVVPLRVTIEGGTNVSSSPSIEYFSQVLLPMLSQKLSIPPITTSLHKRGWSTGRSEIGSVTFDIEPLPQAFVLPTFSFQDRGELAKVHVSVLAPEATARNRIRDKVTAQLLAYSPEIEILFPVDENSGSEKRLYLLLVAETSNGYRLGRDWLFDEKARGLSMEQLCERLVSKVVKDLKKELKHGGCVDEYMQDQTVVFQALAAGKAEVDCGIEREATLHTKTARWVTEQMIGAGFDELGRCQGLGYAVGEDFQKRSGMPLQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN28</accession>
    <name>A0A8H6CN28_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7165 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008992</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154679.1">
        <property type="nucleotide sequence ID" value="XM_037299853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN28"/>
    <dbReference type="GeneID" id="59337387"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055589">
        <property type="entry name" value="DUF7165"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23749">
        <property type="entry name" value="DUF7165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7165" evidence="2">
        <location>
            <begin position="154"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="748"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="934"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1021"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1122"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="770"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="810"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="839"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="879"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="953"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1022"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1062"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1185"/>
            <end position="1197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1197" mass="129122" checksum="B6C1DF330EF3EEB4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNJ8</accession>
    <name>A0A8H6CNJ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009362</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155049.1">
        <property type="nucleotide sequence ID" value="XM_037300223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNJ8"/>
    <dbReference type="GeneID" id="59337757"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="4">
        <location>
            <begin position="174"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="393" mass="43454" checksum="2EB0700585121C7C" modified="2022-01-19" version="1">MSFDAIPVLDLSLSRHPDTKPAFLVNLRNALLDVGFLYIKDTGINDALIQDVIEQGKAFFSLPEEQKLEVQMKNVPSFLGYSKLGNEITALKTDWREQIDLSTPHPLPNPTSPLYHNLLSPNQWPNPKFLPKFQVTFETYISCMSSLSTRFTSLIAESLNLPPSAFDRFFDKDQQHKLKVVKYPDTASLPSSNQTQGVGPHKDSMLTSYLLQVPPHTGLQAQNLNGDWIDCPPIPFTLVVAIGQGLEALTHGVCKSTTHRVLSPQAGLGPRFSVPFFQGVSYDATFESVDIPAEILAMKGERLRQRGGKRIDDVEFTFTKGRWGHLGEATLVNRVKSHVDVGERWYPEILAQVRAEQAAARAKAEEGLAEADASQTREKAAGVTGGPQAIRAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPB5</accession>
    <name>A0A8H6CPB5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155485.1">
        <property type="nucleotide sequence ID" value="XM_037299484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPB5"/>
    <dbReference type="GeneID" id="59337014"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="289" mass="32727" checksum="A8905A43460ECE1F" modified="2022-01-19" version="1">MSDVLAHIRRSNSECIELLRILFTKDELFLHSEVLLFLKSLKEDLAIVSQDVRQMTGGKPMTPEVPPYIPFAFDPDPKFTGRIDVVEGLDAGFSKYRRMALDDPEVNVGKLFGDWLRDPINRPWLMVVDNVDDETLFSSSSRREHTPNSNASLEVHQLHRHLPQVSHGSILITSRNRVAARDITNGDDCIISVDRLPEPDALSLLHKKLPKDNSFMEDAKELVSLLEYFPLAITQAAAYISNGSGRRTISLYLNLFRSDQVGYLEMAANDIRRDSDELDQDFSNSVLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPC2</accession>
    <name>A0A8H6CPC2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155465.1">
        <property type="nucleotide sequence ID" value="XM_037299464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPC2"/>
    <dbReference type="GeneID" id="59336994"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23416" checksum="DC0211D3219D9D23" modified="2022-01-19" version="1">MLGYIFFTFWRLFQIVTLIPTLGMLAYFVSVNQKANRLSPASILTLFIVSTLAAVWAIVTLFRRKSTQRSTLYVAFVDLCFVGAFIAGVYELRGIAKANCSSISDSGPTYLSVGTNGVSGSSGISASVKRDCAMQKASFAFGIMNCIFFFITFMILLFMHRTKDDDNVQYKTEVTRRRRSHDSRRGHSRSGGGGSTHRRSSRDSRRYYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQD2</accession>
    <name>A0A8H6CQD2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155859.1">
        <property type="nucleotide sequence ID" value="XM_037298523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQD2"/>
    <dbReference type="GeneID" id="59336050"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034491663" evidence="1">
        <location>
            <begin position="18"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="341" mass="37599" checksum="DD595C0D6075B46B" modified="2022-01-19" version="1">MLSIILLYLFHCTVVKSVSIRPSNLSQDLSAPKDFTFTDPSIPEPGNTSLANTIPLPINDPHISFAIVKGTAKLPIKACLMVALEALVEMSFEYFTEPYGSGIFVSSDYPEVIIVTNVFKGPGPIFQTRHAARGLVDLITLMLRQSSYSNSSIIMRWSDFGMSALWASVAFAVGDHVATQGPSRAAPRMETLPQGPYLANFSNNNNGTEVVFVNNDDDELLVFAEYVGGALNIPDVFITLYSSVFYIAQFPTTNAMVPFGITPENSNAGLQYILLDPPPRSTFVFQNGWAARSLQKLSKYMFEQRKFNEIKFISGWDKTLFSVGSLKKKTMSGARVDVERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRB6</accession>
    <name>A0A8H6CRB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D-arabinitol 2-dehydrogenase [ribulose-forming]</fullName>
            <ecNumber evidence="5">1.1.1.250</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007878</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; D-arabinitol metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.250" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156082.1">
        <property type="nucleotide sequence ID" value="XM_037298746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRB6"/>
    <dbReference type="GeneID" id="59336275"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047038">
        <property type="term" value="F:D-arabinitol 2-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000240">
        <property type="entry name" value="SDR family oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF103">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="8">
        <location>
            <begin position="166"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="428" mass="46016" checksum="08EAF2AA96B44998" modified="2022-01-19" version="1">MLDFLIPGKPLQSHRPCSASFGTQTLFPSAITPTVHPYPPIPSPTRFPKKKKKEEEEEEEEEEEEEEEEEEEKEEEEAKKKNMINLTRRLRHRLPPSLLPTPNLNRALSAPITPTPKPKPEVPAASHTEPQRRSGAGEVGREAVAFDGGVVPGLTPTMARFTLGGKVAVVTGGARGLGWNMSQALAEAGAQAIALIDVKQDLGDAAAAELRSTAGIPVRFYRVDVRDANAVADAVARITSDLGSVDIVINSAGVVDSNIKAETYDAAMFRNLLDVNLTGSFLVSQACARHMITARAGGSIIFLSSIAGSRVLHPQQQCAYNASKAAVAQLAKSLAAEWAPHSIRVNTIAPGYMDTALNREALLEGQKKHWSAMTPMGRLGRPDELNGLAVFLASDASGFVTGAEILADVSCNLQQLFAEWWEGELLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRE5</accession>
    <name>A0A8H6CRE5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156048.1">
        <property type="nucleotide sequence ID" value="XM_037298712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRE5"/>
    <dbReference type="GeneID" id="59336239"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="405" mass="44195" checksum="D87117E2DE61CEFB" modified="2022-01-19" version="1">MLRLRAANRVSRTICVRSSLRQLRHESTNTSNSSSSSGSSLSQSLIGGLAGGGLVFLGGYTYYHFSGAKNLVNTARKTEDLFKSYGKQLKESSPEPNEALKWLRSSAKSYATFIPGATGYVDSAFDDLDAIRNKHGNEVDNIIKEAYGELKDVSQEGMSVTAATKAWDILQRHLKRIGGLAGDAASDIINNHPALKEKVGGNLDQLKQMGDKYGPEAKKQVDETWDQIRDIIKSGVSASTIPKVQSLVQEKVQKIQELGGKAWDQGMEKAKPYLDKSPEVKELLEKNKEKLKKQGNVQELYEKVKDAVQSGSTDSLKQYVQNTVDKVGQGNGGDGGGLEKYLGMLPGGGEIIPKLTQLQQIAQEHGEEAEKIAKDTFKEIQEVLQKKVGEAEGLAQKAKKNAKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS31</accession>
    <name>A0A8H6CS31_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">acylphosphatase</fullName>
            <ecNumber evidence="2 5">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007941</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the acylphosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="2 5"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156145.1">
        <property type="nucleotide sequence ID" value="XM_037298809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS31"/>
    <dbReference type="GeneID" id="59336338"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029:SF3">
        <property type="entry name" value="ACYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00112">
        <property type="entry name" value="ACYLPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="91" mass="9836" checksum="C2ED1E5F2DDE9896" modified="2022-01-19" version="1">MSQQRISYTVHGTVQGVNFRSFTQSKAKSLNLTGFVANTSDDKVAGEAQGSEDSIKSFLKELNNGPGAAHVVKVEKEEIKTEEGEKSFGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS85</accession>
    <name>A0A8H6CS85_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156335.1">
        <property type="nucleotide sequence ID" value="XM_037298999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS85"/>
    <dbReference type="GeneID" id="59336528"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010513">
        <property type="term" value="P:positive regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024260">
        <property type="entry name" value="Vac7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258:SF1">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12751">
        <property type="entry name" value="Vac7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="645"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="725"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="874"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="725"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="880"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="915" mass="97471" checksum="B1EC1A37198B2467" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F712</accession>
    <name>A0A8H6F712_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Dipeptidyl peptidase 3</fullName>
            <ecNumber evidence="4 15">3.4.14.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 15">Dipeptidyl aminopeptidase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 15">Dipeptidyl peptidase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_006617</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.</text>
            <dbReference type="EC" id="3.4.14.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase M49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.4" evidence="4 15"/>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147225.1">
        <property type="nucleotide sequence ID" value="XM_037297515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F712"/>
    <dbReference type="GeneID" id="59335018"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000002">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005317">
        <property type="entry name" value="Dipeptidyl-peptase3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF11">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007828">
        <property type="entry name" value="Dipeptidyl-peptidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 17" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="16">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR007828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6217790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="703" mass="77790" checksum="FF75A99789C335D9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7J3</accession>
    <name>A0A8H6F7J3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147617.1">
        <property type="nucleotide sequence ID" value="XM_037297043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7J3"/>
    <dbReference type="GeneID" id="59334545"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="182" mass="19320" checksum="1C8AF0592A57D3DE" modified="2022-01-19" version="1">MDPKMSSDTKEGVRDMGAGRLTHDHLHSHQMQLSLSLLAPPLGGTNFTPGLSTGLGSGSGQQAPAPVLPASVSPSPRPATVSATGGTPGRRFYNIRFQTDQIQYLTGVDPLFSNASSNVNVRGGNTKFEFPYTTIRGSGFALSIAQSTTSMSGKYINPGTHTKSVEMVPSRLSKVQVRMVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7V8</accession>
    <name>A0A8H6F7V8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-methanol-choline oxidoreductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006321</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147344.1">
        <property type="nucleotide sequence ID" value="XM_037297219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7V8"/>
    <dbReference type="GeneID" id="59334722"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF138">
        <property type="entry name" value="DEHYDROGENASE PKFF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034825940" description="Glucose-methanol-choline oxidoreductase C-terminal domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase C-terminal" evidence="5">
        <location>
            <begin position="412"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="476"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="520"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="521"/>
            <end position="522"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6217909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="597" mass="63178" checksum="394951A0FA5F2836" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F844</accession>
    <name>A0A8H6F844_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147916.1">
        <property type="nucleotide sequence ID" value="XM_037296735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F844"/>
    <dbReference type="GeneID" id="59334235"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053178">
        <property type="entry name" value="Osmoadaptation_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="519" mass="58247" checksum="B8DC7CD0A80DD93F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8N0</accession>
    <name>A0A8H6F8N0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
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            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148695.1">
        <property type="nucleotide sequence ID" value="XM_037295997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8N0"/>
    <dbReference type="GeneID" id="59333491"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034690089" evidence="1">
        <location>
            <begin position="19"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="242" mass="25169" checksum="F1A91D39B52C90C9" modified="2022-01-19" version="1">MKTQSILSLALAVLGTAAQDSSPFSIFNQTGRLPDGCIDGYFAGSDTVLYTVPYTYSQVLSIIGSYKNLTWSGNPDETVSLNGTDNKVGTARTYEASGAHVVETITVYDKPAVGPYEEIHTLAPLSIPTYNVSFYADYDGTTATPTCNGAATAFNFTINFCATSVSTATAILHMLHMTDAQTVGVFLGNQNFTSCPAINSTSANDTARASTLSNITTGMGSGSTPIAFAGGAGALSASWVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8N6</accession>
    <name>A0A8H6F8N6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005381</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148273.1">
        <property type="nucleotide sequence ID" value="XM_037296291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8N6"/>
    <dbReference type="GeneID" id="59333787"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034323561" evidence="2">
        <location>
            <begin position="18"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="189" mass="20469" checksum="A195233C8A9FBEA3" modified="2022-01-19" version="1">MILLVILLLVPTHLSFAFLPPSNSSLPLLRLPSPPPPPSPPPPTTFTALGPPTTPLHGKPWPSPPYRRPIKDGLAITITAYGDSLAAKYTAYILKALLAIQRVIRNAGGPHDVLEETTTVGKFAGAVYAEVGFYALHAPAGIERVQAAEVLQKVWELVFEYSPAKEITSSTVVAEGRELVLFRLSFRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8S9</accession>
    <name>A0A8H6F8S9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148274.1">
        <property type="nucleotide sequence ID" value="XM_037296292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8S9"/>
    <dbReference type="GeneID" id="59333788"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="215" mass="24210" checksum="BC242CAD5C7A1DBC" modified="2022-01-19" version="1">MPGERTSCEMNLNDDGDMKKTLRALMLQGYIILKDATTVEECWDGAVQDLSNHRLVQEIMTANNEDGFVVLHHECYYMEAGQQPIQHNNSAARRAEPMFLAFHLGGQKGVTKWVITCDEGENGEMTEIYVPVEAVAGDIFLCNARHDRSDPAPCIPKDALCNLIRIQWKIKVTLKFGSLHTLNVTKITSSYVQRIDDIASTYVHQEVPAMHIIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8Z1</accession>
    <name>A0A8H6F8Z1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148558.1">
        <property type="nucleotide sequence ID" value="XM_037295860.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59333354"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="51021" checksum="B354A2AD41B0CABE" modified="2022-01-19" version="1">MYTFANISKSVFGKAQSATPSQPTEVTLTITLEHFDNPSLPKTFATGDEIGGYVSMGMPETTEFDAVSITLEGSRSKTNSRLTGTVTTFVRPPLLSPGGNNRDHKNSQTFLTLKEPVDRWELRRINTNAIGWFYQIPFRFEVPPDQRKSREWQAHLRESVRHAQLKIPPSHEKNSVPSTAPNIASISYCIKAKLLKYGPQDGPENPLGETSRVIRIVPVITEEPPLPICSTLGKESIAAATAYLEGNAWTGSQGRMEMEVAQPKSLCVPVSTGYDTWQKESVETRAEMKLRFEPIEVSGMPPKLTRITAKLRAATRVSSASMSDCPYRIGTRADGTNDGLSVETIPLVGGTVQEVKWHRDLTGHKRDAMSSDQAGGRRHLTTVLPLRLVLPLQKQYLPTFHSSLISHFYVVVLVLSFRMHGDSISPKKESMRLEVPIQVSSELFVNRDSHFSDDSDGEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F963</accession>
    <name>A0A8H6F963_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003747</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149357.1">
        <property type="nucleotide sequence ID" value="XM_037294669.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332158"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF10">
        <property type="entry name" value="OS12G0136300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="3">
        <location>
            <begin position="995"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="757"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1090"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1150"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1361"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1492"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1607"/>
            <end position="2085"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1459"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1546"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="449"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="604"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="645"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="690"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="716"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="789"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1380"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1607"/>
            <end position="1657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1677"/>
            <end position="1689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1761"/>
            <end position="1773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1787"/>
            <end position="1818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1828"/>
            <end position="1853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1854"/>
            <end position="1865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1883"/>
            <end position="1902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1931"/>
            <end position="1979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1980"/>
            <end position="1995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1996"/>
            <end position="2041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2068"/>
            <end position="2085"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2085" mass="236075" checksum="A88668E3BEEEDE98" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F997</accession>
    <name>A0A8H6F997_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148769.1">
        <property type="nucleotide sequence ID" value="XM_037296071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F997"/>
    <dbReference type="GeneID" id="59333565"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051468">
        <property type="entry name" value="Fungal_SecMetab_SDRs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544:SF32">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G01530)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="185" mass="19374" checksum="08FFF209AC4A08A6" modified="2022-01-19" version="1">MGCRSFSKGEAAASSFSNLAIEALELDISSDSSISKAFATVEGKFGHLDVLINNAGISQRALPEGLTTRQRYAQIMDTNAISAACLPETFIPLLCDTLNPASQYYGLDSPEYKASKAALNMIMATNAVELKGEGFKVNVCCPGLNATGLSGAHPSVGPLNASRLAKAGKDGEHGTFTNKEGSLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9F4</accession>
    <name>A0A8H6F9F4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149520.1">
        <property type="nucleotide sequence ID" value="XM_037294139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9F4"/>
    <dbReference type="GeneID" id="59331627"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="186" mass="20472" checksum="D4DBA1DE1EA5CF07" modified="2022-01-19" version="1">MDSGDLASTGDSTSPPDTFSAYIDSDLLTSPRPSDADITSPTTSSRKVYVLTYDAAMWVDKVTLLDGTPIFVAVNDDVSPTAVQPCGVLGTYLSEAKARRIGKAWIYDQLTDLGVHIDLPLPTQDGYVRTEPAWRKSEWRRTADGSWGYSISDYSPSWLALVVYITERVIDDSDGEEEYQDSDNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAB0</accession>
    <name>A0A8H6FAB0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002818</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150572.1">
        <property type="nucleotide sequence ID" value="XM_037293743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAB0"/>
    <dbReference type="GeneID" id="59331230"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000003">
        <property type="entry name" value="membrane-associated progesterone receptor component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF115">
        <property type="entry name" value="BINDING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G06240)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="3">
        <location>
            <begin position="29"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="125" mass="14181" checksum="DA6A10930675DD06" modified="2022-01-19" version="1">MAETGYIEEPKRFAPKDPPKLNPPRDEPIRVEDLAKCDGTDSSYPTYVAIMGTVFDVSSNTAYSPKNPYNVFAGRDASRALAQSSLKPEDCRPEWQDLPEKEKGVLKDWYTFFSKRYNIVGKVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAB9</accession>
    <name>A0A8H6FAB9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002741</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150495.1">
        <property type="nucleotide sequence ID" value="XM_037293666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAB9"/>
    <dbReference type="GeneID" id="59331153"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034840638" evidence="3">
        <location>
            <begin position="25"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="451" mass="50615" checksum="DF972BE0D2858E9B" modified="2022-01-19" version="1">MVIYRQTAHLCILWLIYLSSQSQSSPLPKVEVNQVPETMRLNTENTATSPLREQFKNPAEILGILLLIGGDTIRKAIAQLAGPNVAPVAFSFGWVAYSFGALLSVWGDGRLLPEPDVHSMVINHKSGNTKQNESWVLGRLIRDLEMHFSTQKSKTKEFGGKSFHIIKLEAMDVWKKKHHWSGKDRPDPPAHDLIWWSFFGCVVLQLGLAVAPIFIDHSGNWRILLVTGAGTMLAIIHGSLPQCKEEKYECEMTEKGKDATFILTRGNSHQYVFVIHSPPENRTLNMEHLAVSRRIGGKNVMKYITAVLAACWIVLLIAIGGLEQDTWFLLMVGGIGMFHNLWVAGHARTTSAHGIPLDIENAKPLTDPDKSVMEGLMAAEKIEPGVGLALLKVYFPGRLRPDEDKWWAKKTKRFVKRIENERKKKGKKPKSEEEEESEGSEEESEGADIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAZ9</accession>
    <name>A0A8H6FAZ9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150901.1">
        <property type="nucleotide sequence ID" value="XM_037293248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAZ9"/>
    <dbReference type="GeneID" id="59330735"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="539" mass="55002" checksum="23D48A064D7EB902" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBE0</accession>
    <name>A0A8H6FBE0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Duf895 domain membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151019.1">
        <property type="nucleotide sequence ID" value="XM_037292480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBE0"/>
    <dbReference type="GeneID" id="59329966"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF19">
        <property type="entry name" value="DUF895 DOMAIN MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="55086" checksum="1A9C2C71BE0C4E54" modified="2022-01-19" version="1">MTEILSDPKADPACEDEEKTSNTATLKGEQPSRQSKLSFSSGRNLHTCAVKWYRSTLYNALILGVCNFLAPGIWGGKTPSPEGETSRGLRADQGVISAAMNSLGAAGEEKPYLVNGANALTFCLMVVSCSFSSVIVRLVGIKWALIFGTMGYAPYAAGLYTNNRFGTEWFVLFGAALCGISAGVFWTAEAAIALSYPEPNNQGRFLGLWLSFRVGGQILGGAINLGVNAKNNQAGKVSYKVYLAFIALQALGPFVGLLVNKPSRVQRKDGVRVDMSITNGSLRELQLTAKLFFSKEFLFIVPLIGQGVYTEAVMFTYESLWFSVRARALGSFLSGIVAVVAGNLLGAFLDARKITLKTRARSSFVTVLGLQGAWWIWATVLVTDFHRTKPTYDWVDPGFGRAFALFLLWVVGFQLNYLYLYFVVGHIAKSPAEIVRIAGLLRGTESGWQAISYGVNSVAIMASVGGVYLNFGLWAVSLIPAWLVVGKIGVSKDEGEIENKGVDSASPVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBI3</accession>
    <name>A0A8H6FBI3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PH-response regulator protein palH/RIM21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001423</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the palH/RIM21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151772.1">
        <property type="nucleotide sequence ID" value="XM_037292353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBI3"/>
    <dbReference type="GeneID" id="59329839"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071467">
        <property type="term" value="P:cellular response to pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014844">
        <property type="entry name" value="PalH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALH/RIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779:SF1">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALH_RIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08733">
        <property type="entry name" value="PalH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="384"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="492"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="610"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="672" mass="73727" checksum="962572FB52F61DD9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBQ4</accession>
    <name>A0A8H6FBQ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001971</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6222003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151438.1">
        <property type="nucleotide sequence ID" value="XM_037292899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBQ4"/>
    <dbReference type="GeneID" id="59330385"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF152">
        <property type="entry name" value="OXIDASE (CODA), PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04090)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="536" mass="57774" checksum="88EE201701977D44" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBT9</accession>
    <name>A0A8H6FBT9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001467</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151816.1">
        <property type="nucleotide sequence ID" value="XM_037292397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBT9"/>
    <dbReference type="GeneID" id="59329883"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF5">
        <property type="entry name" value="BTB_POZ DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="264" mass="30127" checksum="F650ABA5B8AD3BA9" modified="2022-01-19" version="1">MTTSQTPSNLSLRELGKGIHRLYKDREHADWTLKTGPSTIERAHSFVLWARSTYFTAACNGKFKATTIRESDMTEYGPFMVHRMLQYCYLLSYDDNPLSLHGVNETYTSKLCTNVHMYNMGDYYDLQGMKLEALNKLKECLKLSEAELAEQDVASLLEVLPWIYSSTLDSDHGLRTVAASFGYRHWETLVARPDFEEVAAENLKWVIDIISQKRGQESKIGQSSEYQDPGPRPRHYTPTPPLPPLPPVPPGFMYPQPNLGFAFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDA6</accession>
    <name>A0A8H6FDA6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Filamentation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000482</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YPP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152856.1">
        <property type="nucleotide sequence ID" value="XM_037291421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDA6"/>
    <dbReference type="GeneID" id="59328901"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051722">
        <property type="entry name" value="Endocytosis_PI4K-reg_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083:SF464">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT DOMAIN 7, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN, TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="877"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="575"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="761"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="578"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="606"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="646"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1043" mass="113548" checksum="E9FB7E2898E6AC40" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDG5</accession>
    <name>A0A8H6FDG5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial resolvase Ydc2 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153106.1">
        <property type="nucleotide sequence ID" value="XM_037301472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDG5"/>
    <dbReference type="GeneID" id="59339010"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000402">
        <property type="term" value="F:crossed form four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16963">
        <property type="entry name" value="CCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039197">
        <property type="entry name" value="Mrs1/Cce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015242">
        <property type="entry name" value="Ydc2_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28072">
        <property type="entry name" value="CRUCIFORM CUTTING ENDONUCLEASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28072:SF1">
        <property type="entry name" value="CRUCIFORM CUTTING ENDONUCLEASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09159">
        <property type="entry name" value="Ydc2-catalyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial resolvase Ydc2 catalytic" evidence="1">
        <location>
            <begin position="100"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="382" mass="42595" checksum="E83DB4B2A050B382" modified="2022-01-19" version="1">MLGSRQVSVASQRNLWIVNFLPTRDWLVDRYTRTIKVWMASRLSWLGSCTNSQLGILARAVGVNSSGTKPILTAHLLDHLPKATVAPYKHRDTLQEPCRILSIDMGIRNLAYCCLVLPRTGSGIATDIGNPVVEDWTRIAISKDLDNSKQAEDLVPNKAIKELKRSGKIKISNPKEAFDPATYSQHAYDLITTLLEDLKPTHILIERQRFRSMGGSAVQEWTLRVNMFEAMVYAVLKTFAERGLWKGTVHPIAPSKVSSFWITNKQGAIKEGPGSKSAKTKTAKIELVAEWLDEGAHFELKGAAAQLGQAYLRKKRRKDSKSLVKQKKLSGSKAQGVAQDSVEKLDDLADCLLQAMAWIKWEKNRRLIMARGAAALGELEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDY8</accession>
    <name>A0A8H6FDY8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153707.1">
        <property type="nucleotide sequence ID" value="XM_037300890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDY8"/>
    <dbReference type="GeneID" id="59338426"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="13"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="335" mass="37767" checksum="C27D0D78EFD201DD" modified="2022-01-19" version="1">MASLHEIRITGDDLGLFDLPNEILIHMLDLLPTQSLLQTRSVCHRFQNLIIRIVHGRLLRAATLTDRKLILECYHPSAQYTEPYLYCDYLGTPGLSDEIEGQGPIYEVADDQAAKQGTLGRLYSHFRPTRKDPEPKIHRPHPAGDVPGSRTSEVANRIREPFERNTVTQNVSLEAHELFMQLRFLVAVVQTGPRRGFFLSVENIIDKTQRIFRNWLAEKAKATEKHMTGASDSVANELGREADNMLWVDQDKKVGLRVRVEERKGRKDSPILVYKDEDQAISYSLELEELIVSTTHLMLAVEKQLLATGGEGNGQAMVFGNFAIPNAQETPGDTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE16</accession>
    <name>A0A8H6FE16_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_011011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153494.1">
        <property type="nucleotide sequence ID" value="XM_037301860.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59339401"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF80">
        <property type="entry name" value="GRIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="522"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1802"/>
            <end position="1824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1880"/>
            <end position="1995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2025"/>
            <end position="2050"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1686"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1898"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1930"/>
            <end position="1946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1974"/>
            <end position="1995"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2050" mass="224114" checksum="4E996AE67168F7DC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEG8</accession>
    <name>A0A8H6FEG8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010063</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153736.1">
        <property type="nucleotide sequence ID" value="XM_037300919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEG8"/>
    <dbReference type="GeneID" id="59338455"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20353">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16630">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047546">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047544">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058758">
        <property type="entry name" value="UBA_RNF216"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF42">
        <property type="entry name" value="FINGER PROTEIN (ZIN), PUTATIVE (AFU_ORTHOLOGUE AFUA_4G03910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26191">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26112">
        <property type="entry name" value="UBA_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="302"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="674" mass="76192" checksum="12A68599886022AF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEP3</accession>
    <name>A0A8H6FEP3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153946.1">
        <property type="nucleotide sequence ID" value="XM_037301129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEP3"/>
    <dbReference type="GeneID" id="59338666"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="322" mass="35538" checksum="F60C1262CBD5D059" modified="2022-01-19" version="1">MAIPLPIPPLRLFEIDDQSWFPAPLRASVQAVLTFLWGYKAPILQPAPPASLVARTLQSVLGPSITDYTFVDFCSGAGGPTPYIEREVNKAFNKLSDGGNILVRNGHTGANGPVNGDGSGGRGGRVDFVMTDIHPHLPEWVRASNKSKDLHYVPSPVDAANAPHDLLSMAGTQEQDKKIFRLFSLAFHHFPNPLATRILHNTLTHSSGFAIFELQGRDLGSIITILGMFPLLWAGSWWWFWGDWGLLFWTYVVPVVPFVLVFDGVISCLRTRREGEVLGLIRECGASMEGWRFETGREVHTRTGGTMNYFIGIKQGGGKKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF87</accession>
    <name>A0A8H6FF87_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1765-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154402.1">
        <property type="nucleotide sequence ID" value="XM_037300554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF87"/>
    <dbReference type="GeneID" id="59338088"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013887">
        <property type="entry name" value="UPF0592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37988">
        <property type="entry name" value="UPF0592 MEMBRANE PROTEIN C7D4.03C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37988:SF1">
        <property type="entry name" value="UPF0592 MEMBRANE PROTEIN C7D4.03C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08578">
        <property type="entry name" value="DUF1765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="917"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1032"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="917"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="941"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="974"/>
            <end position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1127" mass="124110" checksum="D46652D9E9F9C1B6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFG5</accession>
    <name>A0A8H6FFG5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155105.1">
        <property type="nucleotide sequence ID" value="XM_037300279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFG5"/>
    <dbReference type="GeneID" id="59337813"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF156">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="264" mass="29526" checksum="E010110A057930CB" modified="2022-01-19" version="1">MPSISRTFRIPIDGQDPSDTLDIKLHEPSLTGDNLGHKTWVASYLLAKRLPTLLPRLFPGIKPSAEIDPLDPPPPTKYPNNPHRHPIPPPSPHPRTLELGAGTGLVGLAASALFSTHTLLTDLPSILPNLQRNVTANASLTASSAVAAGPLDWSDPDSYVRGDARFDLILAADSLYAPEHPSWIVRVMGTLLRWDEERRARVVVELPFRRERPPEHEDLRVRMREGGFVLVEEGEEVGYDDWEELSPSGGQLEVKCWWSVWRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFH1</accession>
    <name>A0A8H6FFH1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154968.1">
        <property type="nucleotide sequence ID" value="XM_037300142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFH1"/>
    <dbReference type="GeneID" id="59337676"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="539" mass="59882" checksum="E5F491C1C19DB249" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFU2</accession>
    <name>A0A8H6FFU2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155265.1">
        <property type="nucleotide sequence ID" value="XM_037299264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFU2"/>
    <dbReference type="GeneID" id="59336794"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034981521" evidence="2">
        <location>
            <begin position="28"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="26942" checksum="6897B1A1EA6917CD" modified="2022-01-19" version="1">MAFPSISNLPLWFKTLCLLSIARQSECSPVSIVGYNSLVNVIDTASMVNTNVTSSADKSATFTFSSTKLGAPIPNCATLPGYAEWFQPSEKFDNGDCPKALELFFTDYTLDHDGTKYEFLASGVTPVHGIATQRVPLKVAFGTCYVVIAMRNMFVEGELPGETPSKSAGSDISSFAELYQNALEIYAECSQPEVNQPGWYPAGDLNSIAVFIWQAVSEINERVQIPPPTSSDLTPTSGYGSIGDLFGDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFY4</accession>
    <name>A0A8H6FFY4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155189.1">
        <property type="nucleotide sequence ID" value="XM_037299188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFY4"/>
    <dbReference type="GeneID" id="59336718"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05120">
        <property type="entry name" value="APH_ChoK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF15">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="78"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="397" mass="44044" checksum="D695082A68DB1042" modified="2022-01-19" version="1">MYLCLASKVSLWLGIDADFANIQWSVNISDSATDTKSDVPTTRKCLSTRKALQGVLKNVLPAIEGNHNISSEDAAIEDVKVDFLAEGGYNYVWLVSFSSKPSEQDGTTSLHTAILREPNDDAFLPWQIENEVAHLTFIAKDHPSIPVPKVYAYDDGKTGNEPYITMEYIDGQPLDSVWTAYSEDEKLAAARQVAQTIIEMGTITFDRIGGLTLEHEIGPTVEASSPSTFLPEQPFTLVHNDLNGRNIMMRDKKIAAILDWEFAGSYPLSEMLEGRGVDLLETVDDETEEENSAWSLRMGELVAEAARARGWDEGRVELLVGRGNEELGLARIEMVPDFLAEDASDIGAGNDADNDVGGSRGWTALVVWIASFLGDVWRLSNSESLKFWPHQWRLRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFY6</accession>
    <name>A0A8H6FFY6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008533</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155400.1">
        <property type="nucleotide sequence ID" value="XM_037299399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFY6"/>
    <dbReference type="GeneID" id="59336929"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF52">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="269"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="686" mass="76061" checksum="3A55ADE489A5100F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG76</accession>
    <name>A0A8H6FG76_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008576</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155443.1">
        <property type="nucleotide sequence ID" value="XM_037299442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG76"/>
    <dbReference type="GeneID" id="59336972"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042238">
        <property type="entry name" value="Rad28/ERCC8/Ckn1/ATCSA-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202:SF1">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="62"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="204"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="272"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="49829" checksum="074E22EF7B50DB0E" modified="2022-01-19" version="1">MTSNLFGRALGSISPHAVQKAQTSRLLNSLQAAPGIRFDGSTGAALTKAQQHGDEEDELGEVWAHKAGVNCLAVDRFEGRYMLSGGAESSIILWDLESAECSLNRCTYNPSVKASKTSSAHKFGITHLSFYPFDSLAFLSTSYDHTLKIYATETLTPSASFDLNLVVYSHALSPIADHLLVACATQHPAVRLVDLRSGASAHSLAGHQGAVLSVAWSPHDEYIMASGGSDGTVRLWDIRRSAGSLGVLDMDDSVGVGGEDGRGKNARTRNHGKAHIGACNGVVWTEDGRHIVTAGHDERVRVWDVGVGANALSHFGPIIKNTHLSTLLPLLAPRQVSGSQVMLYPNEKEILMFDLFEGTLLNRLRAPGVAVAQASGSAGKRNIRNRVTSLGWRNGNVEVYSAHSDGLIRAWKPRTKADIQLEKDEAEQVDEDEDESRKRKRQALDDVFRDLTRQKITFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG93</accession>
    <name>A0A8H6FG93_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_008622</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the U5 snRNP complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155488.1">
        <property type="nucleotide sequence ID" value="XM_037299487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG93"/>
    <dbReference type="GeneID" id="59337017"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17945">
        <property type="entry name" value="DEADc_DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000322">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057479">
        <property type="entry name" value="PRP28/DDX23-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25430">
        <property type="entry name" value="DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="385"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="416"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="630"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="316"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="782"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="385"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="317"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="785"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="796"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6227180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="821" mass="89991" checksum="51A7B84262FA0356" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG98</accession>
    <name>A0A8H6FG98_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155324.1">
        <property type="nucleotide sequence ID" value="XM_037299323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG98"/>
    <dbReference type="GeneID" id="59336853"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="365" mass="42133" checksum="8C24399BCDD8B7B1" modified="2022-01-19" version="1">MLRPLGDNKLNVLLRPDDDTDPYRISAAKQQLGQWRQLKDGLSVLFDIWPSSFEARLHHDKIDFEKWHIYFRHVETFEILDYPQNWVAMLKWIWNYGYERQLTKVKSGSMLPLLDVETYNAIAQDNSDAGCSGIRRAKASFEDKNLALGVYYMLEGFQFRMPLLHRDTNLEKWHVDFKGTPFRSLRIQPTTDHGIVAMYAFDQKIGSFKILDFPARGPYVGDLVSRQRMIAWIWDYGNKRLEGRVKSKAAQRSQPKAPTKTHAKVRRTEKPLLHRVLHTASFKAQRGASSNIQGTRTVPKKGDITMEDVGAGAHRENVEPSARKAPLRVADPNAQLGMAWQTKGRGHFRRQQYLRSIISKLSDWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGA6</accession>
    <name>A0A8H6FGA6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155775.1">
        <property type="nucleotide sequence ID" value="XM_037299774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGA6"/>
    <dbReference type="GeneID" id="59337306"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="116" mass="13200" checksum="E66BB488BB575881" modified="2022-01-19" version="1">MTSSFASSSHGTRVYVTFNSDEAGKFEVATVELDTSDRTRHPGVVKTRMFETPERPASFTIKLDKPYHDYANVVARAAVTKAALQRGFVLNLRVYVIFNTSKDRFMKVDAVERRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHK8</accession>
    <name>A0A8H6FHK8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">KOW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006973</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156504.1">
        <property type="nucleotide sequence ID" value="XM_037297869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHK8"/>
    <dbReference type="GeneID" id="59335373"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000009">
        <property type="entry name" value="60S ribosomal protein L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6228862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="137" mass="15563" checksum="90CD791325567394" modified="2022-01-19" version="1">MTKINTSLHSSRRKSRKAHFSAPSSVRRVIMSAPLSKELREKHNVRAIPIRKDDEVTITRGSNKGRDGKVTSVYRLKYVIHVERVTKEKSNGTSVPMGIAPSKVMITKLKIDKDRENILERIKEGREAKAKRGSKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHV3</accession>
    <name>A0A8H6FHV3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_008184</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156388.1">
        <property type="nucleotide sequence ID" value="XM_037299052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHV3"/>
    <dbReference type="GeneID" id="59336581"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="246"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="452"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6228454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="634" mass="68193" checksum="F2497B658942D8E8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIG4</accession>
    <name>A0A8H6FIG4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable kinetochore protein NUF2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Probable kinetochore protein nuf2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_007276</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NUF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156802.1">
        <property type="nucleotide sequence ID" value="XM_037298167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIG4"/>
    <dbReference type="GeneID" id="59335675"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051383">
        <property type="term" value="P:kinetochore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.60:FF:000003">
        <property type="entry name" value="Probable kinetochore protein nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.60">
        <property type="entry name" value="Ncd80 complex, Nuf2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005549">
        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041112">
        <property type="entry name" value="Nuf2_DHR10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038275">
        <property type="entry name" value="Nuf2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18595">
        <property type="entry name" value="Nuf2_DHR10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="13" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="17">
        <location>
            <begin position="32"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Nuf2 DHR10-like" evidence="18">
        <location>
            <begin position="283"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="170"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="373"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF18595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6229160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="465" mass="54593" checksum="B45FB684F70CE7F0" modified="2022-01-19" version="1">MDYNSARMSQQFRSQSQPTGGRGKQQQIDPDVMMRLPDREIVGCISDIGVQFSLEDLQKPNPQQIQKIFEWFAELLMNTTRETVEPAMRAAAEDVCGEHMEIVPPDTRNLMGFYVSLRKLLVECGIADFSFNDLLKPTHDRLVKIFSYVINFVRFRESQTATIDEHFNKAERTKSRIETLYLENQDMESRLDEMRRERKSMDQQVKEKTKRNDELKQRLLELKKGQERVAERLERVRGEKGRLTGVLEEKTERCLGVRQESEKLRPYVLQSPAALQANLTELSENLGRDRSQIDSLEKRTRALQTSTDTFGVVTNDVVSCTKILEEISNELHKEDEENLKAAKRRDALSERGNNVREVERTEAVLQRHLKRWLERTETVRNGSREKAQSAKEKMEELRSVHRRLTEERVDKGREMEKRRVRIEQTEKKMADLKENIEDEIHSAHDQYLKMDSHVKLYMNEIEHVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIQ8</accession>
    <name>A0A8H6FIQ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007382</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156908.1">
        <property type="nucleotide sequence ID" value="XM_037298273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIQ8"/>
    <dbReference type="GeneID" id="59335781"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF17">
        <property type="entry name" value="DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="6">
        <location>
            <begin position="208"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="814" mass="91098" checksum="487BE22AD616667B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIV8</accession>
    <name>A0A8H6FIV8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157016.1">
        <property type="nucleotide sequence ID" value="XM_037298381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIV8"/>
    <dbReference type="GeneID" id="59335889"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="192" mass="21786" checksum="8FF44A2FF4CB1F74" modified="2022-01-19" version="1">MPTWWPEFAREAAINETSDTTLKSEELKKHTSLSIPRNSTILCSSDNNIPISLRVSPPLPTFTWPTENTFTALPTRSSPMYVSTSKTHTIESSERLWEVEEILQMRLVRGQGLRAKRAKRYLVRWTPSLLSAEKLHYAQKTWKIVKTSPGIHTDKGGAFYQKIKVQWAPSWVSSLDFGPAMGAWETASHLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKG1</accession>
    <name>A0A8H6FKG1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004518</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157436.1">
        <property type="nucleotide sequence ID" value="XM_037295435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKG1"/>
    <dbReference type="GeneID" id="59332926"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090324">
        <property type="term" value="P:negative regulation of oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052000">
        <property type="entry name" value="ETFRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024:SF0">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN REGULATORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE SOLUBLE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="71" mass="8391" checksum="2F3198DD978F4532" modified="2022-01-19" version="1">MWGRPRRTTAKLKPRRFAELLNLGKNYPQGYPYFQTHLHKAFASQTGLQDEKDIRKGIERAEYVKKEIEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKX8</accession>
    <name>A0A8H6FKX8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004768</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157683.1">
        <property type="nucleotide sequence ID" value="XM_037295682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKX8"/>
    <dbReference type="GeneID" id="59333174"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056599">
        <property type="entry name" value="AAA_lid_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054289">
        <property type="entry name" value="DUF7025"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411:SF2">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23232">
        <property type="entry name" value="AAA_lid_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22942">
        <property type="entry name" value="DUF7025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="752"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="990"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1104" mass="125902" checksum="F8E33E70E3ECBC24" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY73</accession>
    <name>A0A8H6KY73_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007089</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156618.1">
        <property type="nucleotide sequence ID" value="XM_037297983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY73"/>
    <dbReference type="GeneID" id="59335489"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034690048" evidence="3">
        <location>
            <begin position="28"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="371" mass="40794" checksum="144143478AA25522" modified="2022-01-19" version="1">MAAPLKHWRSWSPFRLDALGLLTMLGADEMSREIGSLSANTLVSWMSLLGGNVVPSDAFVAPLHGYTLYNISDAIQSTSLAGWFSRWLLAQHPSQNTSLFVWSTRSYPRHHGHRALAIGTIVNTCILALSLLVGDYYGLASALCMDFGIIIRAYLVAQNRDAIDRAAADTSIHSHDLVRTFIILANGKATTVIAPRNVVVNCFLSTPKIAHPTFYAIARAMGWFAFVGLIVTVGQAVLVVQIAIIIVTVSATVLTAFGIGTVGDGVGKSLQVARSEHPNRHGRRGEAYAMLHLNKEEEDTMIDWALFPQRRNQNWWNQYRTYKAIDSKIIDVFEDSATEEKTLKASSFRGSTLTEEKSAATTAKVQELKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYN9</accession>
    <name>A0A8H6KYN9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156902.1">
        <property type="nucleotide sequence ID" value="XM_037298267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYN9"/>
    <dbReference type="GeneID" id="59335775"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="564" mass="58255" checksum="0E7765F1CDE4A1F8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZH1</accession>
    <name>A0A8H6KZH1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable beta-glucosidase btgE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Beta-D-glucoside glucohydrolase btgE</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Cellobiase btgE</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Gentiobiase btgE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_004199</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Beta-glucosidases are one of a number of cellulolytic enzymes involved in the degradation of cellulosic biomass. Catalyzes the last step releasing glucose from the inhibitory cellobiose.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157119.1">
        <property type="nucleotide sequence ID" value="XM_037295118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZH1"/>
    <dbReference type="GeneID" id="59332608"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF24">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034503806" description="Probable beta-glucosidase btgE" evidence="14">
        <location>
            <begin position="20"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="64"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="65"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6229862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="373" mass="39060" checksum="34F75611876A7AFC" modified="2022-01-19" version="1">MKGIIYLGAAVALLRSVAAFLPTNHDDYDDQDQVILQPAKGLNKAASTVAESTEHIEAAIPAQRFASNSPRPHDTPSQNPGQSPSTHDTTLHPSQPQKSNFTIGSASSQWAIAYTPYTSTLSCLSASSIRSDVATIARKGFTSIRLYSIDCSALRHVGHAARTHGLKLIIGIPIDDGLADAETQLSELIAWAGTPADNQWSLIELIVVGNEAIFNTHATAPALATFITTSRATLRAAGYAGPVTTTEPLSILAQHRGTLCPALDLAAATIQPFFHPSVPASAAGAYVRSELARLAAVCPGLPAVCLETGWPSRGRPNGAAVPGYLEQWMAVAGVMAEGAGGRSVVLGFGDEGWRDEGEFGVEGSWGCGHVFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZP8</accession>
    <name>A0A8H6KZP8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cystinosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_004426</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cystine(out) + H(+)(out) = L-cystine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:66172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35491"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:66173"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystinosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157344.1">
        <property type="nucleotide sequence ID" value="XM_037295343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZP8"/>
    <dbReference type="GeneID" id="59332834"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000016">
        <property type="entry name" value="Cystinosin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6230087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="33055" checksum="F0F3E130DAE1F517" modified="2022-01-19" version="1">MPTSYWPEPGKLEALSSSSPSSVQFLRAISRLLGWIYTLSWSASFYPQPLLNIRRHSTYGTTPAFPTLNVLGFFCYTTSTVCFYYSPLIQSQYRTRNNGEENTVRANDVAFAVHALALCILTLSQFWHSLWGFESRKARPGMGVWGIVVGCIVGIGWVIGMVMGKGLDSGRDPKSWAWIDVVYAFGYVKLVVTVLKYIPQARANYQRKSTVGWSINQILLDVIGGVLSIAQLVIDSSLQSDWSGLTGNPVKLGLGNVSIIFDIVFMVQHYILYKGNEVEEDGMDWERRGLIANEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C519</accession>
    <name>A0A8H6C519_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146918.1">
        <property type="nucleotide sequence ID" value="XM_037297717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C519"/>
    <dbReference type="GeneID" id="59335221"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="254" mass="28434" checksum="33AC0E5A9E52E6D1" modified="2022-01-19" version="1">MTSDEKLTLVQAIIETTDKHDFVSPQLFADSASQRERQEVEAARTYILQQAFPTIQTLTPLCNTKALSYTIRLDTVGSFLKPQPHMLITQDPGGLHVAEVNFHTHGYDTTIVYKDVGISQRLALQNLQEQNFECLINGKPHLWHPLGPSKSVFELTEEAKKRVALFVYAEGMAQRTGSTSANGMPVQEQKIGEVHVMEDFLHEPVALQQTLFSAIVVVEQAKRRATSIASWVPPPASKTHFPGRRRSRQDGKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5H3</accession>
    <name>A0A8H6C5H3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146932.1">
        <property type="nucleotide sequence ID" value="XM_037297731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5H3"/>
    <dbReference type="GeneID" id="59335235"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="246" mass="26871" checksum="CD1720BBC11F9F0F" modified="2022-01-19" version="1">MIFSPAPQIQAGPRTATASHRDDRMVDGSAARRADRMTLLEHGAMAADLVDIVRASTTEIVVVVCVVPSSVPSRVTLARPAPLRLLARASRMHTSSIASNTLIASVKFNLGDGGEFDSGERSEDEELGGVFWRLKRLAKSCGWGRGGDQDDEHKDDENVGSSSKNKGKKRVPDNDDPETLIEMLALIAGEGDVTKVAEEHKTRGINCPGHLHRLELLQWSHEALGEDWQKKRNVEASKSKVKFTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6K9</accession>
    <name>A0A8H6C6K9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTP 3',8-cyclase</fullName>
            <ecNumber evidence="5">4.1.99.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_006362</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the MoaC family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147385.1">
        <property type="nucleotide sequence ID" value="XM_037297260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6K9"/>
    <dbReference type="GeneID" id="59334763"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013483">
        <property type="entry name" value="MoaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02666">
        <property type="entry name" value="moaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="87"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="44"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="507"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="712"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="519"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="755"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6217950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="789" mass="87299" checksum="4D3A5D7C1A1BB7E1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7E8</accession>
    <name>A0A8H6C7E8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147454.1">
        <property type="nucleotide sequence ID" value="XM_037297329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7E8"/>
    <dbReference type="GeneID" id="59334832"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="196"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="258" mass="28111" checksum="8E9900323468C8BC" modified="2022-01-19" version="1">MDFPMDDHFDFDAFINWPDNVSPAHDGSFSPIGWPMTQTPLGSATFGHAQPLAEAIPGYSANTANDDTDQVTAACIPPQDVVDTPSLGQESSSTNPSKECACMVCLGIGIFESNSPGPTPYLCRFAGCSWDPRYYTGVTEKLEYRAKHERPHYRRGPANSQTPFYCLVENCRFSSKRWSDLRRHTTAKHCNSPNTFACSVIGCKYHGEGNGFTRKDKLTAHYKNMHQGQKIPGQAARAIKPAPASSHAEASGSSSIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8H3</accession>
    <name>A0A8H6C8H3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005895</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147981.1">
        <property type="nucleotide sequence ID" value="XM_037296800.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59334300"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001433">
        <property type="entry name" value="ABC transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF119">
        <property type="entry name" value="ROA1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="502"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="533"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="630"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1118"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1144"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1175"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1259"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="63"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="700"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1314" mass="144480" checksum="C5D95DEBBF488324" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8P9</accession>
    <name>A0A8H6C8P9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005452</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Forms a heterodimer with SLX4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SLX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148344.1">
        <property type="nucleotide sequence ID" value="XM_037296362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8P9"/>
    <dbReference type="GeneID" id="59333858"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033557">
        <property type="term" value="C:Slx1-Slx4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03100">
        <property type="entry name" value="Endonuc_su_Slx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027520">
        <property type="entry name" value="Slx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048749">
        <property type="entry name" value="SLX1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050381">
        <property type="entry name" value="SLX1_endonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20208">
        <property type="entry name" value="STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20208:SF10">
        <property type="entry name" value="STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21202">
        <property type="entry name" value="SLX1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="10">
        <location>
            <begin position="40"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="399"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="402"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="451"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="462" mass="51977" checksum="AF13E799DE8D8209" modified="2022-01-19" version="1">MFGGKYPVVPVTRYPNQQFPALSPEYLPNLNAETDTLNHYEAVTFAIQQQRHSSLYVGSTPNPARRLAQHNGLSKGGAVRTSRNNLRPWEMTLCLMGFPSKIAALQFEWAWHNTHITRRIADEQRITTRETKVRTFTKTGPTRKRPARPRTSLIDKLSNLHLLLRVPSFSRWPLQVRFFCEDVYQVWQGWNERVDGSIRSGIKVLLDLKQPEETINDREPLMSTQAKGKRKREALGKGGIEGLDVGYSRLKNHVRKSTLFLTKDEDLKCAVCTKNLGPKTAMALLCPQEDCRTASHLTCLATKFIEDEKASAAVTPISGRCLGCKAELQWIDLIKDLSLRARGEKEVAQMMKKPKERKTKVPKTKNAGALHLEAHTVIEDVADPDKDFVEADMRALNASDESLPDDWQYQDDDDDDMMSVMSGHSALSDGVDAANATKRSLTAPKLPAVIEDSDWDDAEPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9F0</accession>
    <name>A0A8H6C9F0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat and J domain-containing co-chaperone DNJ1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005188</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148797.1">
        <property type="nucleotide sequence ID" value="XM_037296099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9F0"/>
    <dbReference type="GeneID" id="59333594"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000083">
        <property type="entry name" value="DnaJ and TPR domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000224">
        <property type="entry name" value="DnaJ and TPR domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051727">
        <property type="entry name" value="DnaJ_C3_Co-chaperones"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140:SF2">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034365723" description="Tetratricopeptide repeat and J domain-containing co-chaperone DNJ1" evidence="9">
        <location>
            <begin position="23"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="34"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="403"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="466"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="479"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="502"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6219362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="529" mass="57674" checksum="2BD8B4FF8422E626" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9J4</accession>
    <name>A0A8H6C9J4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003903</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148872.1">
        <property type="nucleotide sequence ID" value="XM_037294825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9J4"/>
    <dbReference type="GeneID" id="59332314"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF129">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="36"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="490" mass="53285" checksum="D77351E8543F6900" modified="2022-01-19" version="1">MASLPTQNSTASFWHSEPSHFLLGHRTTPDLPTEADIVIVGSGITGASAARFLAEDARAKRLRVVMLDAREACWCATGRNGGHCQPLLFDSTPDVAAFELRNYNAVKSYIKKHNVPCEWRSLTGCRTFWTEPLAKAAADDVRDLKRHAPELGKEVTVIDDEESLRRHRVNGAPGATLSACAGSLWPYKLIAFILEDLIKEGHLNLQTKTPVTKIEPCTDDPEGPAPTGPRHILHTPRGTIKARHVILATNAYTSHLLPEFADLIVPERGVMTALLPPAKSKPLEDSYGFVGALGGNPIHDDYLNQRPFSGVPNPAGHLMFGGGYVGKKLNMIGETDDSVLDEGSASYLREALLKLLILGGETGGLKELKMTHQWSGIWGTSKDHHPWVGAVPSRPGVWLAGGYSGHGMPNGTLCGKAVVEMLLAQEAGVAIDDVQNRLVTDGNLPQAYIITQDRIERCKHIDSVQVQDRKGIVGIRSLDAMIQAQWNTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Q6</accession>
    <name>A0A8H6C9Q6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148962.1">
        <property type="nucleotide sequence ID" value="XM_037294915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Q6"/>
    <dbReference type="GeneID" id="59332405"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="215" mass="23124" checksum="1B51986040AB4A8D" modified="2022-01-19" version="1">MVMECAERVVAIRSKITYQIHVSYGSETGAVSMLVDPVIVRRIDCLTALNGSDVDLLTSVERLSGVRMQIPANAELATDIRKAIEHNIRHLAVTASISFSVVGGEEPKDPNGSNNLAEKVAPKGYISLQEHHDAMPRASAIPRRHSTTSFTGNDSGTESCSTVGATPPSDEASDTFLMSKGRSSDLIRLNLQLATLTQLLILKRGVGRLTGRLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9U1</accession>
    <name>A0A8H6C9U1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149022.1">
        <property type="nucleotide sequence ID" value="XM_037294975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9U1"/>
    <dbReference type="GeneID" id="59332465"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179">
        <property type="entry name" value="PROTEIN CBG02620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179:SF2">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="226" mass="25146" checksum="663F9D886C0344DE" modified="2022-01-19" version="1">MATVRRSGTCIVSFQSLLKVALHHRIVRYRFRGLGLLVRSEVDAYLQERMKGPDSPKKANSGDLLNHADFVKTLSLGSRPTTPTETKEKPSSRVTVVQGGHNVPPAAVLELTTRSEPAKRTSDIEQKLADLWFSQTLYFIEAYYRSVGYILYNQLRLQQGKFEDIKAKSIGDQLTKWKTANAIGLRKLVTVLGGIVEEVKKAQGPCIVRSLILLAMLVQGIEVVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA86</accession>
    <name>A0A8H6CA86_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003602</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149212.1">
        <property type="nucleotide sequence ID" value="XM_037294524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA86"/>
    <dbReference type="GeneID" id="59332013"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="128"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="128"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="412" mass="44885" checksum="E498883F5EF152B3" modified="2022-01-19" version="1">MCDCQNPLLSPVAKLDIRDLIEESPSPRSRTTQPSAFSRSSSFTSPHTQYSHRPSSSASCSLSHYSAGTAPPNPFGRQPAHGSQQLPPQPPPPLSNTAQAVFQQNPGLNPAAASAKRPPPSGSHSSDSSRKNRGKWTPAEDAKIIVLRGRGMKWDDISVEIPGRSAISCRLHYQNYLEKRADWDEEQRNKLARVYDRLKSDLWNPIATELGVPWRACEAMHWALGEADMCRRAHVLPFPMATSRPEPASAPAPSAGRVAQRDRMRGSTRVFEGRDITTITSPAFHSVNGNGNAQGGHGPPYAGIPSPMSLPAIGTGHGFPGYEEEEEQDDDDNDDDDERRQEGPRVRRRRLGSATRLPGVAELDRTIAADLAASSEAGRRVKLEEPGRRVKHEHDRRGSGGSGGSGASRRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAF2</accession>
    <name>A0A8H6CAF2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149051.1">
        <property type="nucleotide sequence ID" value="XM_037295004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAF2"/>
    <dbReference type="GeneID" id="59332494"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="319" mass="35843" checksum="B4D932A5FC75282D" modified="2022-01-19" version="1">MAWVPAHEGYKEHVMYQPRRWYNGFHEGPDQRSEIGPGNPLVHFPGVEQKFNAMARYLDKVETLELVKVLQVPLSNMSLQDDIDGFWLRVRRAREAVGRAENELLFLKEPSLGDRFRKLIDELKRLLFEEAYTEESMIQATLAVEAALSAAADHKATSGEVPDNPGNSIADDNNEHTFDPDLHLRALSDTVRSAWFTDNLTLQLYRNPNISTSAYYAESRTPVLDSPNACSETQTPSPIPQSSVQSSTSQAAATKPQFQYTPIKKSTAYPSFSRLHYLNAQQQTLDSSLTLSKCRLSAPQFSQSAIQSQNPKSRPRIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBI4</accession>
    <name>A0A8H6CBI4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003297</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149601.1">
        <property type="nucleotide sequence ID" value="XM_037294220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBI4"/>
    <dbReference type="GeneID" id="59331708"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="131" mass="15051" checksum="3CEE2EAB21442539" modified="2022-01-19" version="1">MFNNYDKLAKNDALLHNPAFAMKKKEAFDKFYARFSATIAPLNYSESHKIAALRRLITLKLRSRIAGPQRSPYSEQLKDQLKKEGRCFKCLGYSHRPNQTEAYMASKALSFEEAKAIMAKEKAVEKAMEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBN9</accession>
    <name>A0A8H6CBN9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150040.1">
        <property type="nucleotide sequence ID" value="XM_037293962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBN9"/>
    <dbReference type="GeneID" id="59331450"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47098:SF1">
        <property type="entry name" value="PFKB FAMILY CARBOHYDRATE KINASE SUPERFAMILY (AFU_ORTHOLOGUE AFUA_4G09500)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47098">
        <property type="entry name" value="PROTEIN MAK32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="1">
        <location>
            <begin position="208"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="438" mass="49172" checksum="5F58D8594306841A" modified="2022-01-19" version="1">MPEKHLIPEKEQVNFTGEDEIETPIDFVTLGSCIIDDIYQESPPKLLYSNVLGGAGIYAALGARIFREIKGNGVDSKSVGMIVHEGCDFPVDAKVELQSWCLSSIFVRTPHRLTTRGKNVYSKEGVRDFEFLTPKIQVDHAMLSGRFLTSKSYHLICTPIRCIELVKGIMSRRKALKNEHPQWNSDQFQTPFIVWEPMEHCCRPSELQSIFEAMDFVDVFSPNDRELFALFNEENQGEGTIAQQDLQQYCKTLLAQGFGLKPSAVVVRMGEDGCFVASHTRNVALPAYHTPLKHVKQEDRATWKNRVTDPTGGGNAFLGGYCIGLLMDEWRDLGPGLTAFEGSALVGSISASFAIEQSGMPTLSYHEGKELWNGERSLDRLDVMRERVEKLDITKVSGELFDKFSPWEVSKRSAATGTTSQRLLHRRSTIKDARTRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBS0</accession>
    <name>A0A8H6CBS0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003513</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149816.1">
        <property type="nucleotide sequence ID" value="XM_037294435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBS0"/>
    <dbReference type="GeneID" id="59331924"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF157">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="453"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="489" mass="54401" checksum="40CB0477CBB3F188" modified="2022-01-19" version="1">MVDSSSYEKRKDPHNELNNHGLQLGNDGFVEWKLDEKQHPRNWIITKKAYNVGLVCFFEFWMTAIRSIFCSPISETFGRRTLYIVAITVYCIFSAIIAAVPSVIAVCFGRFFTGFASAIPATVAFGSFEDLYESETRIWIVYIYTLAGNMGLVLGPIYSAYVTTYAGWRWVFYISTIVSGIGIIASLFLHESRTRYLLELKVKAVQKRAGNNALRIAERQNITLRSFAQDCLFRPLKFLATEPIVFSCAALMAISFSLIYGLTEGLNVVYTELGFAESTTSSLSFVPILIGLLVNVLPRIYDQRVFTRCRKKSSLRPEAKVSSLVTACPALAVGLWLFAWTIPPLVPHVHWTVSMIGLVLVGYATNDLSYILFGYLTDSYGPYAASACSALSLSRTLMAAVFPLFTYSMYTSLGGNVATSIFAAVATMACVTPVVFIKYGRKLREMSHFAQADGCENQRDEETPSRKEEEKVERGQRDSSASTVILLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBS1</accession>
    <name>A0A8H6CBS1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SRR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149779.1">
        <property type="nucleotide sequence ID" value="XM_037294398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBS1"/>
    <dbReference type="GeneID" id="59331887"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080">
        <property type="entry name" value="SRR1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080:SF1">
        <property type="entry name" value="SRR1-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRR1-like" evidence="1">
        <location>
            <begin position="108"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="266" mass="30002" checksum="AA2C033A90F9C7A3" modified="2022-01-19" version="1">MMYVATWRMHHRIEQREGKLMVQGGHYVPETWGRKFGEVAGEDEWGMGEPQHLRHHDANPIPPTFEPLSLSELPSEYATWTQQFAPTPFAAALARTISHEMPPPPTITLTRCLCLGIGNFAQGAVYPFDDESDQRRYRSPHQLVFLAFLPTVLGERHRIRKREVYFQDPDFGAVEVEFLQRRLGYTVLSDPGAYAKMTPSTLLFAPCVPHWVRAGALEVALPALYVSVDVGRDLERMGPGYECSLTRVPCRVGKGMFGRSGLMCVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC30</accession>
    <name>A0A8H6CC30_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150177.1">
        <property type="nucleotide sequence ID" value="XM_037294099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC30"/>
    <dbReference type="GeneID" id="59331587"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="56"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="122" mass="13315" checksum="35D50B346EB2BAAE" modified="2022-01-19" version="1">MATPAAQRSSHNTDAEYNLPNDTVEHNRLEDQAAGFADLMHNRVIHAPLRNPHRLLDIGCGTNAVTHHLAATYPTASHIYGLDLSPVPHIHRPRAAPTANVTYIQGDVKKLSRTDERLAAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCK2</accession>
    <name>A0A8H6CCK2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002643</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150397.1">
        <property type="nucleotide sequence ID" value="XM_037293568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCK2"/>
    <dbReference type="GeneID" id="59331055"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="74" mass="8544" checksum="2F0320D17BCEBBFC" modified="2022-01-19" version="1">MSKYPEVQVVLGWEPADASTQPLEDYMYRWGESTPDPAFEDGKTAFCVYTYEQPNDLESVISQSFRILRTVLDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCW2</accession>
    <name>A0A8H6CCW2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcriptional regulatory protein RXT2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150485.1">
        <property type="nucleotide sequence ID" value="XM_037293656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCW2"/>
    <dbReference type="GeneID" id="59331143"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039602">
        <property type="entry name" value="Rxt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013904">
        <property type="entry name" value="RXT2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28232">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RXT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28232:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RXT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08595">
        <property type="entry name" value="RXT2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcriptional regulatory protein RXT2 N-terminal" evidence="2">
        <location>
            <begin position="38"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="349"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="560" mass="61440" checksum="388C9C5A62BADD17" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD25</accession>
    <name>A0A8H6CD25_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MAP kinase kinase kinase</fullName>
            <ecNumber evidence="11">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_002700</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Activates the PBS2 MAP kinase kinase by phosphorylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with by SSK1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150454.1">
        <property type="nucleotide sequence ID" value="XM_037293625.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331112"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051403">
        <property type="term" value="P:stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06626">
        <property type="entry name" value="STKc_MEKK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000482">
        <property type="entry name" value="MAP kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017240">
        <property type="entry name" value="MAPKKK_Ssk2/Ssk22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037579">
        <property type="entry name" value="MAPKKK_SSK22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1068"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="197"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1228"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1358"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1231"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="1097"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6221019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1390" mass="156318" checksum="C70F5F57E06DE504" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD53</accession>
    <name>A0A8H6CD53_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin reductase like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the flavoredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150588.1">
        <property type="nucleotide sequence ID" value="XM_037292935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD53"/>
    <dbReference type="GeneID" id="59330421"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798:SF5">
        <property type="entry name" value="FLAVIN REDUCTASE LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798">
        <property type="entry name" value="FLAVOPROTEIN OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="5">
        <location>
            <begin position="111"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="321" mass="35200" checksum="84A75DD0280E7357" modified="2022-01-19" version="1">MLEKEQMPEDHCCPWVRPKEEISPGKAIVSEHKAGSKTTFVSKHPDFKKVEATRPDFNHNAPIDVTKSPDPSWRYGQGVNDGGASLQKKHVEIDPFAEGRPMMSNYKLLVSGIVPRPIGFISTLSADGKTANLAPFSYFQVVDHDPPMFIVGFSSRPGPSKDTYRNLKETGECVINIVSENMLPAVNATSIDAPYGVSEWAVAGLHQAPSATVAPSRVQESVFSIEAKLTDMKEFENHAKPGMSIAALGIVEATRFWVREDAIDEDRSHVDLKVLRPVAQLGGISYARITETFELPRSNWDAAVKEESWLGELPGAKDASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDD8</accession>
    <name>A0A8H6CDD8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150738.1">
        <property type="nucleotide sequence ID" value="XM_037293085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDD8"/>
    <dbReference type="GeneID" id="59330572"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="122" mass="13765" checksum="F9519726A3BDD963" modified="2022-01-19" version="1">MHIPQPAHCEDESCDRVPPPAVVLNRCPLLTSRAITNGSLGFIVSYESSLVTPNDKILELDRWCFHGNAEEPHAESILEYGSACRRCHTFDNEEFDERIAVAEEKLAREDRPDRIPEFANTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDJ9</accession>
    <name>A0A8H6CDJ9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150894.1">
        <property type="nucleotide sequence ID" value="XM_037293241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDJ9"/>
    <dbReference type="GeneID" id="59330728"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034740363" evidence="1">
        <location>
            <begin position="18"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="316" mass="33193" checksum="BEB3A8FE1FA9998D" modified="2022-01-19" version="1">MLSIVLAIILPTTFASAATPTNETADCDFTNGTLNVYQQPNASSSYSIPAVVSIGSTVVNSTTKTWQIINTIGNVLDSSNVVDARAQQLSQYIFLDTSSTINGSSSSSPLPFAGCSFTFGLPSSYIGKTSSDGTCGEIFGQSCLNEIMQIAQDTTATIPPAVGLTTLIDACDTVSETINNYIATSSKACSKHGPSIEVGQGINFSYANNENCTNPPPTLPIQLFEQYEPFHQGNYTTYDEWTKTTTPILLALFSNDTTLNGAGGSYSQAYLTCTSPMNVTAGSHDPTSAADHSAVADVNSVVGFALAIIALVFLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDK5</accession>
    <name>A0A8H6CDK5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150890.1">
        <property type="nucleotide sequence ID" value="XM_037293237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDK5"/>
    <dbReference type="GeneID" id="59330724"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="211" mass="23118" checksum="621DD77FD8530D2C" modified="2022-01-19" version="1">MPKTNSPGASSPVMQTPSKRTSSPTGDPSAQLISELRASVEKPKSEPKDRIIRTPKSRGTNVHSGLAGISVTNAMMREESPPTDAAMGSRHHPWLHLTSYELLTLRKRMKKNAIWKPSETMVAAELIDLGRGFDNYLAAKKQAEAEGTEFVDEANIASTKPELFINPTTPGGRPINRGMVLNAAKKRKREGLSELVDDQKESLSKKVLQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDT6</accession>
    <name>A0A8H6CDT6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151077.1">
        <property type="nucleotide sequence ID" value="XM_037292538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDT6"/>
    <dbReference type="GeneID" id="59330024"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="54176" checksum="D0D1D0FA2A67DDF5" modified="2022-01-19" version="1">MASVPPELLHLIVSQAALSYVYCPKKGEVTKDLGTLRSLRLANRKLSKVASEYLFEELTLYSTEASHAKMMAVAQHPTYSAYVRSIGISQKAIFGPFLDRYAFGQWFHQARPLVMCDGLPKGYFYVPHRMAYLPTKEARVIDFHHAEYTSLYKKQEQLFGKAGDLLKTAIGCFSHLELVEPSVRTPRTTYPVPSTDDAFISDLWQDSACFYEYDLEHSAMILTALYQGKSLAATQINISELFYKMDTMVMDLPDPVASGEIQKLVADAKKVNLSIQTSDYTGLQKLLNTSKCEKFLGLMKNLESLACSTYELQGSPLPYPLLFDIFGHNTWEHLHCLDLGRFRTPAIKLAKLLGRHSSTLEKLTLQHILLSQGSWLDIFIKVRHGALRVVQLHHLGCGNDTDEFFDDVDALELSPIPSSHPLFAFLFREASWVPYMEKVLEGSELDSEGSRSDSQGSRPDSEGSRPDATDWEDMDSEDDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE88</accession>
    <name>A0A8H6CE88_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151026.1">
        <property type="nucleotide sequence ID" value="XM_037292487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE88"/>
    <dbReference type="GeneID" id="59329973"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="195" mass="21758" checksum="DD9DC78E06C2D68B" modified="2022-01-19" version="1">MAKNGRKCAVKQSDDSAKPAEMKLLTLRHDADLWYRIRVLLHDLSNIGNDPSAEKRLSSTMHELYISAPYFTESEAIKVRTTLIDNTTEATTIEDQKHPSLSPCEKATLEEAIHNRLANFFDKRKASGDSRPCGPHDMVPIYVSAFGIGKEELEDEKFLSRLRRSGLGDFKSPNSAASRQGESYMKGQKKGGKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEA5</accession>
    <name>A0A8H6CEA5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein rds1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001587</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151056.1">
        <property type="nucleotide sequence ID" value="XM_037292517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEA5"/>
    <dbReference type="GeneID" id="59330003"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039254">
        <property type="entry name" value="Rds1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38705">
        <property type="entry name" value="PROTEIN RDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38705:SF1">
        <property type="entry name" value="PROTEIN RDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13668">
        <property type="entry name" value="Ferritin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034758224" description="Protein rds1" evidence="2">
        <location>
            <begin position="20"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="40074" checksum="F30E3B40754E8213" modified="2022-01-19" version="1">MFTRSAIAAILAVAPAILAAPAPAPQVTEPASFGPDVQVSYQAQSTAGPDYPLAEELGDGYTSATYHGPYTGTPTTTGAANGPTTLAASIPPLPLNPTATYYNTGGKPINPLPAPYTPAGGLGTNGTLPRYMVESDFDYESIALGLYQEWIELDLFHDGLARFSEAEFLEAGLTSEYMSLIEFMADQETGHSTLLTNMLGETAPKQCVYDYPYNTVREWLDFMQRLTRFGESGVWGYINHLDSREVGQLLAQSIATEARQQQIFRQMSGLTPMDVWFENGWPQSWSWTMLAPYISYCPENQTRLAWQNFPVLHILNNANINRYSPNDTAQDGSEVTGSRITDPSISNLTDWTQQIRTPLLPFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEE0</accession>
    <name>A0A8H6CEE0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phenol hydroxylase-like C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001634</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151101.1">
        <property type="nucleotide sequence ID" value="XM_037292562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEE0"/>
    <dbReference type="GeneID" id="59330048"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012941">
        <property type="entry name" value="Phe_hydrox_C_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038220">
        <property type="entry name" value="PHOX_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07976">
        <property type="entry name" value="Phe_hydrox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phenol hydroxylase-like C-terminal dimerisation" evidence="3">
        <location>
            <begin position="82"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="289" mass="30853" checksum="BF6713765D9C992A" modified="2022-01-19" version="1">MHVKDFASSSCHHGVGHVDEGKHKSVDVRGPVREGLKHFSASLSKGFCNGYGGSELFNIVFVTMCLPFEIAERTTDTELRTLGDVATIVYDDRLLDEDAHYTWGIDHAKGAVVVVRPDLWVGISAFPDETARLKHYFGNFLLPQSEATEAEKAPNAMPADRTEADTDVGEPTNDHLVDGSVPDSTANGTTTGEEAKEATTSGSSAPWLLPCSWGKATKEAAVGGSEADSNARKEPMDELKDKVAMDAAHDGPAFEEAMIEIPVNEPGASTDGGKGEYFVTESASVNATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEQ0</accession>
    <name>A0A8H6CEQ0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151509.1">
        <property type="nucleotide sequence ID" value="XM_037292090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEQ0"/>
    <dbReference type="GeneID" id="59329576"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="172" mass="19058" checksum="880AB0880501601D" modified="2022-01-19" version="1">MAALNTKSRANYEEWLESIDDNELKSFVFANTAAEVEFGSDDFKLVYSGVSEGEPVDCHMIEARVNGKTKDKQLKKFVKTTGSLAKIRTCLAFALFPRATADYCLGNYVREALQASPVKDKVVVNKVHVQLSPSVEWEGDQPLAEVGTPPFVFEKIPFPGYKVDGTTRGCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEQ6</accession>
    <name>A0A8H6CEQ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001218</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151567.1">
        <property type="nucleotide sequence ID" value="XM_037292148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEQ6"/>
    <dbReference type="GeneID" id="59329634"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025016">
        <property type="entry name" value="DUF3955"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13127">
        <property type="entry name" value="DUF3955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3955" evidence="8">
        <location>
            <begin position="62"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="201"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="290"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="438"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6222132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="51835" checksum="93ABE2590AA9B791" modified="2022-01-19" version="1">MSQPLAPVPADDFVLPVPNSRSEDSQAGRRSTDSSWSQRSRMSSHKTPPPHQKPLRGVWRHTIGIILLLATVVLWTASNFLASTIFADDSYSKPYFVTYVNSSFFSLLLLLVAAKRLWATGGSIKGAIRGHDRSTHYLPIVEEEEQAFIKPDDDGGIQEASRSPRSRLLIEEPSKSSGVEGSPSERRLNVRETLKLSFEFCILWFLANYFVAACLEYTTVASSTILTSTSSVWTLIFGALLSVEKFTIKKLVGVLASLAGIILISSIDITGNQDKNRGSFPHKSHKEIAIGDVLAFTSAVMYGIYTTLMKKRIGDESRVDMPLFFGFVGLFNFITLLPGFFILHFTGIETFELPPTRRILAIVLINSATSLVSDLCWAYSMLLTSPLVVTVGLSLTIPLSLIGQMIEYSQTSSVSYWIGACIVLLSFVFVNHESTQNEEMGKPEEEDSLGKPSSLLQRTWGRVRPQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF82</accession>
    <name>A0A8H6CF82_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151809.1">
        <property type="nucleotide sequence ID" value="XM_037292390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF82"/>
    <dbReference type="GeneID" id="59329876"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="13036" checksum="8588BC7B0E0B7BA7" modified="2022-01-19" version="1">MPDASSKSPTTKLVAVRKFVEPQYLEPPFSTCQNELDAPMEVWRPIHLNCIQLADEVDPVGDGAMALYIYAVGAPFETILNNYMITSQAIHPPGVGSVSNGTLTGVTTNGSLELAWALQDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGR6</accession>
    <name>A0A8H6CGR6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152449.1">
        <property type="nucleotide sequence ID" value="XM_037291014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGR6"/>
    <dbReference type="GeneID" id="59328493"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="284" mass="31890" checksum="763D58E353CAC4C0" modified="2022-01-19" version="1">MLWYICLEEVILNEISSVNKKGPVRTSSLSLPQSSAAILNPRFGRIILVSPTSTMATSLENFDVCVPNDKASLYKFSREVRDEVYRYLVKGTYIIAVPPSLTSAARFTATGGVGYDPDGADFSILRVSKTLGQEAIGVLYAESIFEFYLNFSEDGPCTQYAIKVTGRMMRFKYQMFGLGAPFQSLFDLSRLSRPYLENMKDICDSTIDHFTSTDITRHSMQIILRVTRELDDTLSSPLFLAMKRLEGFRTLRVELRSSLPIFEKGPPARKRPLERGITVGRGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGV7</accession>
    <name>A0A8H6CGV7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152330.1">
        <property type="nucleotide sequence ID" value="XM_037291968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGV7"/>
    <dbReference type="GeneID" id="59329453"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF4">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="323" mass="36099" checksum="91BAD1E0956D552B" modified="2022-01-19" version="1">MTSSQKAITKVVLGAMSFGRKNVAPHCRVTDLDSGNSILDVFQKHGHTEIDTARRYGNGTPETMLGDLKRQERSLVISTKDLPAFHSNPETAIPEGWDFDQHYHPDMLRLTLQKSLAELRMNLLHVYCFGLDGSVPQSNIFCGINEVHQEGLSAKLGLTCLPTWEVAQICELCDREGWVKPSVYQGKYNVLHRTVEPELFPCLRHYGIKFYAFNPIAGGFLAGGESHERNKNGSYGNEKYFAAMELPDPVVEKHGSTLRECALRWLAHHSLLGVGKGDAVWMDASSAAHLESNLEKGPLPDVVVGALDRGWERVRGVRWKYWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH06</accession>
    <name>A0A8H6CH06_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001111</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152405.1">
        <property type="nucleotide sequence ID" value="XM_037292043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH06"/>
    <dbReference type="GeneID" id="59329529"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43669">
        <property type="entry name" value="5-KETO-D-GLUCONATE 5-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43669:SF4">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="219"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="541" mass="59447" checksum="D418BBCC9C915C93" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIJ8</accession>
    <name>A0A8H6CIJ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153192.1">
        <property type="nucleotide sequence ID" value="XM_037301558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIJ8"/>
    <dbReference type="GeneID" id="59339096"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013169">
        <property type="entry name" value="mRNA_splic_Cwf18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31551:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31551">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08315">
        <property type="entry name" value="cwf18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="23733" checksum="F70B55732DB15298" modified="2022-01-19" version="1">MASHASLDAAATERKARLAKLKSLKRKQPPTDSEDQDTSELGPSIPTEAPPTSTEDPTSQYLSGRNYDPETRGPKLGFESAPNAEQETLEDRAAAIALETREKEERERREEKPIDLFTLQPKKPNWDLKRDVARKMEVLNARTENAIARLVKERVEGQKRKEVETSNGDVGKGQDGESVGMGGSELVEGVHLREKEEAEEERREREEDAELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIQ4</accession>
    <name>A0A8H6CIQ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED4</name>
        <name evidence="10" type="ORF">HO133_010902</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIQ4"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019258">
        <property type="entry name" value="Mediator_Med4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13208">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13208:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10018">
        <property type="entry name" value="Med4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="203"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="273" mass="29574" checksum="A613F7511F67E776" modified="2022-01-19" version="1">MNNIIQAQLDRVETALNTLIESIASYNPSIPAANALLAADDDLNEGLQQLSTHQHNHARIQSLRLTIDQQNASITDTLTALASTRADLLSIPSSLPQKDARNVPYNELLDYAKRISRYTVPPAFRHALPPAQPAIPAAVNGDTVAVEEGAEDSKEEKEGMGTASLEDIERKWLDPLTQIPFVPWVSDETIKRGALAEIQSMVERGDDPENMGAGGSKEEKGQTMEIGESEDAAGKEGDISADGGMGMQRREEKPKVFGGLDLYDPDSPDVDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIR6</accession>
    <name>A0A8H6CIR6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_010917</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153400.1">
        <property type="nucleotide sequence ID" value="XM_037301766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIR6"/>
    <dbReference type="GeneID" id="59339307"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein arginine methyltransferase RmtB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein arginine N-methyltransferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
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    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
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    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6224340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="594" mass="67298" checksum="3BD10E9BE4A872C3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ16</accession>
    <name>A0A8H6CJ16_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037301686.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
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        <location>
            <begin position="285"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="339" mass="36759" checksum="81636C8A71706C87" modified="2022-01-19" version="1">MTSPKDEGERKLTKAGAGILGAQKHKELQQGIEAGEGLAPLSARPNKRRAEDSDTMSVGSSRASKPKAGLSKKRRLLDLDEPDASDSGSPRMLSENPSLKSASKGSKMTDVLSEANRPKVLPKHLPGLKSSDDQVHLPAVLSLSNGTTITLTYERTTAQASEEDEDFWEQVKQRVHQPQQVCSDEVDGTMLSSIKSLEFHNREIISRLLSMIPGTHIAAEEHNKIVRHIVASTNEAAELTRHELCSYGVSIATMAYQAFRDQSIIKTIGVHMLELLDPATAKQASTTPGKQKQEAAAHDKQQGIKQEPGEADFLGENNGGGDLLSDGVGKQEQQHYGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJE2</accession>
    <name>A0A8H6CJE2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_011002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153485.1">
        <property type="nucleotide sequence ID" value="XM_037301851.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59339392"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034765859" evidence="1">
        <location>
            <begin position="23"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="308" mass="32667" checksum="A1BD725604DFF3C3" modified="2022-01-19" version="1">MLTQHHLTAVAAIISFAHFINAIPTTYPPSPHAQSVLYSWSTEPVCTTTASETDCTTAYAALCARAELGVSDNTTVGDCTAFYWYDAGNTIPTAAECTAAYTQILAVSIGGALGYNVEKSRTNDPLYAIYPKDGNANCFKAAGDTSPVLAVDALPNGGGTLTTCPVSTSRRRRAIEVLEGRDQFEPENQNDGVIECAIEDGVWNALCTGVCLAAVTSTSWITGPFVVAGWLACLGGCGATASKEFNNCQKAKGNTDPDELLPLNPFGKRQEATESSNPCVNIKNWSYQCPTQETELLSVYSCQGTINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJL5</accession>
    <name>A0A8H6CJL5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009937</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153610.1">
        <property type="nucleotide sequence ID" value="XM_037300793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJL5"/>
    <dbReference type="GeneID" id="59338329"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF23">
        <property type="entry name" value="MOSC DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_7G05900)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="MOSC" evidence="2">
        <location>
            <begin position="263"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="445" mass="50155" checksum="1215DC0161D4D683" modified="2022-01-19" version="1">MSFQSLGFATHVPRIDPSLTTYIAISVACFVPVLLWGLLKASCLHSRRDKPQGCRKLGLGSTSYLADEFDERYKGAVAGQHKGSATWRVKSLWIYPVKSCRGVELETGTVIGTGMEYDRQFSFAQLSSKFPVSANTPREEKAKHTWKFITQRTLPPMATIKTEVWVPDSSSPTYSEWHPNIRSGGVLVIKFPHIQCEEGFMGKVKDLLALVSAQPEKEVHIPFNPTQEQISDKGYAVDDMEIWKDKPRSLIIASTEKDDRWIQELRSYLGITNHFALFRVSKDDQPREVYRCAPRKDKLGYQPTVGFQDAYPLHILNLASVRDVGDKLQKGAPPLSARNFRPNIVITGGGAYEEDCWKRIRIGGYQYYVSCRTVRCLLPNVNPITGKRHGSEPNRTLKDFRCIDAGDPKNACLGMQMVPASEDSRIRVGDEVNVLETGEHLYIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJP2</accession>
    <name>A0A8H6CJP2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009967</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153640.1">
        <property type="nucleotide sequence ID" value="XM_037300823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJP2"/>
    <dbReference type="GeneID" id="59338359"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="30"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="369" mass="40992" checksum="FC46B6BAB08BB149" modified="2022-01-19" version="1">MAVSPEPSRGPGLAALCIVMIVLSTLAVILRIWSRLTSKKQRFWWDDWCAIMSLPLVLATLSTILLLVPTGLGRHKSQFDPEKLSLALKYSYVHELLYQPAITMPKYSALLFYVRVFEIKWDSRLFRTNILVCVGLVTVWFLFSLLFDVFQCTPIQKVWAPLIPGHCINTSHWFLGLATVSVIIDVYIMLLPLPILWSLHAGRKRKMILTGFFFCAYCVITCSLGRVVAIVQLNKKSDGDLLLAELAISIVSICLPNMTQLLRRAHQHGVSALFTCREYPAGTGSRSKKGLAGFALLQGNKGGFQCVVGNDDTTLLANNDPLINKKDQSGLYRASASAQQSEEGAITALGQVRQRHDIDVRENGGWAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKF2</accession>
    <name>A0A8H6CKF2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010452</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasmic vesicle membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
            <orientation evidence="1 6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="6">Cytoplasmic face of coated pits and vesicles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the clathrin light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154122.1">
        <property type="nucleotide sequence ID" value="XM_037301305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKF2"/>
    <dbReference type="GeneID" id="59338843"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000996">
        <property type="entry name" value="Clathrin_L-chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639:SF7">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01086">
        <property type="entry name" value="Clathrin_lg_ch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="53"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="254" mass="26819" checksum="AB456871903B7FED" modified="2022-01-19" version="1">MADRFPPLEDFSAGDTGPTSNGASGHDGATAGDDFLTRERAALGDDAAQFASAGDNAATVEDGDDDLLGGGGSYGGAQAGSEEITAFESSFPAMDTRNENVGPGGTITGSSLPFQPAQPQPSYGGYGAPAEEETEPIRQWRERRDLALAEREKISASKKEDTIKAARQAIDDFYENYSQKTEKTVAKTRKDAEEFLANREDTSAGGTSWERIAKLVDLSGKGTKGGASGTGKEKFREMLIDLRKDEKAPGATGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKI6</accession>
    <name>A0A8H6CKI6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010451</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154121.1">
        <property type="nucleotide sequence ID" value="XM_037301304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKI6"/>
    <dbReference type="GeneID" id="59338842"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="163" mass="18047" checksum="844C43D2D33109F4" modified="2022-01-19" version="1">MTLHVLEKLIVAQRKSVSETRDHVRALILKLALTKARSEDPLLRILEALASLERAEAEDFRSRGQSITDLVYNLINVRNAQAAQRGADAAQQESRDLGRISWITFISGPLIAVSGIFGMNVDVLINKPPSIRWYFVAVIPFSALVLAVAFVSIRVNKRRGAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKR9</accession>
    <name>A0A8H6CKR9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010105</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153778.1">
        <property type="nucleotide sequence ID" value="XM_037300961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKR9"/>
    <dbReference type="GeneID" id="59338497"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003884">
        <property type="term" value="F:D-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="D-AMINO ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530:SF11">
        <property type="entry name" value="D-ASPARTATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="42"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="269" mass="28924" checksum="74769A5E0F64AF13" modified="2022-01-19" version="1">MYLEIVPSTLYWSSSASEISPQLWWRSIVENFSYLPANQFPEGIQSAVGFTTICINVPRHLNYLLSTFLAMGGKLVKARLPTDKGLPGALRFAASLAMSQEPHERLICVNASGLGAKALVPDDAMFPVRGQTVLVKGEAKYAKTMNENKYVIPRPGSGTTILGGTREVGNWSTEVSNKATNAILEGCKSLAPELLEKDGSFEVLSVQCGLRPAREGAPRVEAEIVDGIFKTVHSYGHAGAGYQNAIGSARKVVRLVKGLDLEASTSAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL08</accession>
    <name>A0A8H6CL08_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009463</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154172.1">
        <property type="nucleotide sequence ID" value="XM_037300324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL08"/>
    <dbReference type="GeneID" id="59337858"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050447">
        <property type="entry name" value="Erg6_SMT_methyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025774">
        <property type="entry name" value="PiNMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068:SF11">
        <property type="entry name" value="GERANYL DIPHOSPHATE 2-C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068">
        <property type="entry name" value="ZGC:194242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51581">
        <property type="entry name" value="SAM_GTMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAM motif I" evidence="4">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAM motif III" evidence="4">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="333" mass="36957" checksum="46F839DB3A191A4B" modified="2022-01-19" version="1">MEVHHEAPIAHTQSSSKDSEAQDIGDDYEIPPPEPSTSFAGLKDRIKRHYEICSDYYYSLWGEHIHHGYFLNPADTKEVAQTRLVDLLLERSQLPPGSQVLDVGCGIGGTSRYLAKNKGCSVTGITISGQQVKLAKDLSAKEAGRVQGEVETSSSIGLGNGTVRFVELDAEKMGDFFNESETKTAFDCVWISEAMSHLPNKELFFRNASMLLNEGGKLVVADWFKAEELTDAQMEADIKPIEDGMLLPGLNTQSEYLSYAKQAGLSVFSKPFDISKEVSKTWDISWSLVQNPALWAFAISQGRDGLAFLQAFRAMRRGYANGTFRYAVMVFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL64</accession>
    <name>A0A8H6CL64_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ysc84 actin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009488</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154197.1">
        <property type="nucleotide sequence ID" value="XM_037300349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL64"/>
    <dbReference type="GeneID" id="59337883"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11524">
        <property type="entry name" value="SYLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF8">
        <property type="entry name" value="DUF500 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G07310)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ysc84 actin-binding" evidence="2">
        <location>
            <begin position="218"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="683" mass="73813" checksum="073B46FEBA608438" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL90</accession>
    <name>A0A8H6CL90_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010363</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="4">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the annexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154033.1">
        <property type="nucleotide sequence ID" value="XM_037301216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL90"/>
    <dbReference type="GeneID" id="59338754"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0001786">
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="4"/>
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        <property type="entry name" value="Annexin_repeat_CS"/>
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        <property type="entry name" value="ANX14"/>
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        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF102">
        <property type="entry name" value="ANNEXIN B11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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    <dbReference type="PRINTS" id="PR01813">
        <property type="entry name" value="ANNEXINFUNGI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0041">Annexin</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
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        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="727" mass="79804" checksum="7BFEF2D9C90A511A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLU0</accession>
    <name>A0A8H6CLU0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">feruloyl esterase</fullName>
            <ecNumber evidence="2">3.1.1.73</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009624</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>feruloyl-polysaccharide + H2O = ferulate + polysaccharide.</text>
            <dbReference type="EC" id="3.1.1.73"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.73" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154333.1">
        <property type="nucleotide sequence ID" value="XM_037300485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLU0"/>
    <dbReference type="GeneID" id="59338019"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030600">
        <property type="term" value="F:feruloyl esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043595">
        <property type="entry name" value="FaeB/C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38050:SF2">
        <property type="entry name" value="FERULOYL ESTERASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5038889624" description="feruloyl esterase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6225624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="345" mass="36980" checksum="8306805AAAFCBC3A" modified="2022-01-19" version="1">MTPLNPVLLTLTLLLFSALALALSNLSPGCANPQPPDSPTPGTPSTHQTIDVPSAGGLRTYLLSLPLNPSHAPSPLILAFHGKGQTASQFETQTQLTDPEFRLRGSIVAFPQGVHGQWLGDPTSPPRSERDDIAFARALLKQLMQEWCVDLTRVYLVGFSNGGGLTHLLACDPEVSAQVAAAAIVSGAFYKDRSLKGDGPLFGTCNKGEAPLPILEMHGGADPVIHYDGKSTPDGETYPLTEWLGDWRERNGCAGAQSELKRDIHDGSVQKTEWWCGIGKQGAKDVVVHYYIRGFGHGWPSTKGQDDDGQRYGPVGWNGTSDIVGFLAGKWLGGGEVMEGTRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMN8</accession>
    <name>A0A8H6CMN8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009104</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154791.1">
        <property type="nucleotide sequence ID" value="XM_037299965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMN8"/>
    <dbReference type="GeneID" id="59337499"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF49">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="11">
        <location>
            <begin position="238"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="158"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="172"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6226238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="533" mass="54682" checksum="E52AE4DC984A7A94" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN76</accession>
    <name>A0A8H6CN76_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154869.1">
        <property type="nucleotide sequence ID" value="XM_037300043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN76"/>
    <dbReference type="GeneID" id="59337577"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="318" mass="36988" checksum="D637D8A9C6439624" modified="2022-01-19" version="1">MVELHETMRTAQPLATRKYSRQLRISEETWIRRRKQSTPSKENLDYRDKQLRGIASQFRYERIPERLWLREGVFFPFSERSYPAKWTEADCEAWFNYRISVLRGQCNKDNDIILNADILMQVALRRLLGMAQEIKGEPDPDHPGENLWAVRGRVGLELWPYDCTPCTASIAHKIHGQEMTTGYWQGDEYPDWRTWDAERCNILFESWCWNCEKFNFPAELEDEKIERHLSLPEGADSQINEIWEIRSPADVRSDPAFQTTCSFDVKDKVTEENVAVALTFLGCDVGSTIPDGIEAVWRLLDVEGGSRGKLEEYLLSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNV6</accession>
    <name>A0A8H6CNV6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-alpha-acetyltransferase 40</fullName>
            <ecNumber evidence="4">2.3.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_008298</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-seryl-[histone H2A] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H2A] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50600"/>
            <dbReference type="Rhea" id="RHEA-COMP:12742"/>
            <dbReference type="Rhea" id="RHEA-COMP:12744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-terminal L-seryl-[histone H4] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H4] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50596"/>
            <dbReference type="Rhea" id="RHEA-COMP:12740"/>
            <dbReference type="Rhea" id="RHEA-COMP:12743"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. NAA40 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.257" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155166.1">
        <property type="nucleotide sequence ID" value="XM_037299165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNV6"/>
    <dbReference type="GeneID" id="59336695"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043998">
        <property type="term" value="F:histone H2A acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039949">
        <property type="entry name" value="NAA40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="13">
        <location>
            <begin position="77"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6226857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="268" mass="29835" checksum="5C8A020BBF56B842" modified="2022-01-19" version="1">MYLPPHEPDSPTTPHTANAPIILPSTLPPPPAPAMPPTSRLATLNELPLPTFLAQLWPRDDPRVETPGPADGGPWRVRLRASDGLSEAELTACFRLVRDSSAADYGASASGWRPGRKREEMRLRDLRYMLLGRSAAGGEPEGEECLPVGGFLSFMLTWEDGIEVIYCYEIHLDESVRGRGVGKRLMGHLEEVGRKAGVEKAMLTVFKRNKAAMQFYERLGYEEDEYSPRPKKLRNGVVKELDYLIMSKSLIGGVKNHQEEGQRKRKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPL6</accession>
    <name>A0A8H6CPL6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein PBDC1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008447</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PBDC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155314.1">
        <property type="nucleotide sequence ID" value="XM_037299313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPL6"/>
    <dbReference type="GeneID" id="59336843"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3560.10:FF:000001">
        <property type="entry name" value="Protein PBDC1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3560.10">
        <property type="entry name" value="yst0336 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023139">
        <property type="entry name" value="PBDC1-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008476">
        <property type="entry name" value="PBDC1_metazoa/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021148">
        <property type="entry name" value="Polysacc_synth_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410:SF9">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04669">
        <property type="entry name" value="PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polysaccharide biosynthesis" evidence="5">
        <location>
            <begin position="21"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6227006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="158" mass="18870" checksum="1B0396431E1939DF" modified="2022-01-19" version="1">MSLGPTGASLNPEQAENFEDMEKQFAVKAVQHMTTYWAILEKMRGSKLRLTKMDDEIYEHFKNEFPEFDPKETIDEDKMKSKEGKERWRNFMMTYEKTVEDYNFGTMLRSNPKFEYGEKETIFAMRMQFYAIEIARNRKGLNDWVYEQAHPNEDSSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPV8</accession>
    <name>A0A8H6CPV8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155735.1">
        <property type="nucleotide sequence ID" value="XM_037299734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPV8"/>
    <dbReference type="GeneID" id="59337266"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021102">
        <property type="entry name" value="PNGase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056948">
        <property type="entry name" value="PNGaseA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31104">
        <property type="entry name" value="PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25156">
        <property type="entry name" value="PNGase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12222">
        <property type="entry name" value="PNGaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034394732" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="2">
        <location>
            <begin position="57"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="687" mass="73802" checksum="461883D637CA4F37" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPY7</accession>
    <name>A0A8H6CPY7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7708 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155701.1">
        <property type="nucleotide sequence ID" value="XM_037299700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPY7"/>
    <dbReference type="GeneID" id="59337232"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056125">
        <property type="entry name" value="DUF7708"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24809">
        <property type="entry name" value="DUF7708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7708" evidence="1">
        <location>
            <begin position="190"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="243" mass="27247" checksum="108D90E2DDC3A466" modified="2022-01-19" version="1">MGLEIKREKDASICSFDLGINKGHQKLVTWDLTVTGLVRLPMSSGEDEEDGLSLEGLLPRPPRPRRKVFLEKLAIGRRKSTGQEFLHSKHLPGGSPIIADPESQVRRVTRSHSNANVHGFKAQWGDALVSQEPIQTSLSQLRQTVVVYEKQASNEKWGELWRIRADVAELDTLARAALDKQKSRKTPFKGLERFSTVALESSKLLDVIMNQCPEYVALAWGVTKLLLVANINHSRLKQNVESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR00</accession>
    <name>A0A8H6CR00_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155917.1">
        <property type="nucleotide sequence ID" value="XM_037298581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR00"/>
    <dbReference type="GeneID" id="59336108"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034247206" evidence="1">
        <location>
            <begin position="21"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="209" mass="22539" checksum="B9BDFBA6854E1D08" modified="2022-01-19" version="1">MRGTIASICILLLNATLVKMAPATVGARIDSGTSIVQLSDVLPSVQNATSNLSSSFCSKNNKNSDLERHIISIPYTTTILIILYCPVVPIDRNSIRQTIAAASTHMNGQIRIHGDISLLPADDPYATPVVEDVNCIMSVTSQRTRTQSIPYRLTYEITLNALQGLNKFLYTDNHAASAVTEVLDPGLTGSMRRIGVISISPVQALVNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRD9</accession>
    <name>A0A8H6CRD9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">M-phase inducer phosphatase</fullName>
            <ecNumber evidence="3 13">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_008014</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the MPI phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3 13"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156218.1">
        <property type="nucleotide sequence ID" value="XM_037298882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRD9"/>
    <dbReference type="GeneID" id="59336411"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01530">
        <property type="entry name" value="Cdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11711">
        <property type="entry name" value="GINS_A_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21692">
        <property type="entry name" value="GINS_B_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000021">
        <property type="entry name" value="M-phase inducer phosphatase cdc-25.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031633">
        <property type="entry name" value="SLD5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038749">
        <property type="entry name" value="Sld5_GINS_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF17">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16922">
        <property type="entry name" value="SLD5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00716">
        <property type="entry name" value="MPIPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 13" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 13" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="15">
        <location>
            <begin position="370"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="171"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="242"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6228284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="736" mass="81696" checksum="67FD4F8CC6FA5BAC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRK4</accession>
    <name>A0A8H6CRK4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008023</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156227.1">
        <property type="nucleotide sequence ID" value="XM_037298891.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336420"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052575">
        <property type="entry name" value="SSU_processome_comp_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046523">
        <property type="entry name" value="UTP20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011430">
        <property type="entry name" value="UTP20_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695:SF11">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20416">
        <property type="entry name" value="UTP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07539">
        <property type="entry name" value="UTP20_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 N-terminal" evidence="2">
        <location>
            <begin position="866"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20" evidence="3">
        <location>
            <begin position="1691"/>
            <end position="1909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="842"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2423"/>
            <end position="2446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2578"/>
            <end position="2619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="842"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2429"/>
            <end position="2446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2594"/>
            <end position="2605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2606"/>
            <end position="2619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2619" mass="293279" checksum="D81201891A8EA480" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRQ8</accession>
    <name>A0A8H6CRQ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156317.1">
        <property type="nucleotide sequence ID" value="XM_037298981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRQ8"/>
    <dbReference type="GeneID" id="59336510"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="24"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="338" mass="36830" checksum="0F2D1FDA55BF4E75" modified="2022-01-19" version="1">MAAAFLSQLLRVENSVEPNSSERCIVCLTECGTLCDESGIVEWEMRLPCDHKVGSRCIATWLDPTGAANNSCPVCRYVFFPAQPRPYLEHGIIEEDHFDMSDSGEYDGPGSPNYNADNAWTFNPVNRDFGRLYYHGAEDDDDQGWGPDIGGGNIPGSPDYSPRSSDSAPRSPVSSNLEHRGNTMAEEEDDDDDEEVSEEDIHEGASVTSEVEYRSGTDLQTVKAMCETYSYRLNFTSSSKTIAVSQRFAEKIHVACALAGHAPPSVAAVAVFVASHLAGAPKTAHWVSLMSGVDADAIAGLYWFVCPARVRAELVDEEMLAMIDRGDRETVLGFLPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRZ6</accession>
    <name>A0A8H6CRZ6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156452.1">
        <property type="nucleotide sequence ID" value="XM_037299116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRZ6"/>
    <dbReference type="GeneID" id="59336645"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="13697" checksum="2D25329BC913BAC4" modified="2022-01-19" version="1">MTKNVEVGTFTKTAIVFDRVKLLDVDILLKDIGLLSTPFELRFYSDYLGEGMREAHEKGCRARKEAESVPSLIVEFAETIETLVVKSRDLWGFKVVLRVEFIDSDGDPELMELGRRASIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7T6</accession>
    <name>A0A8H6F7T6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147825.1">
        <property type="nucleotide sequence ID" value="XM_037296644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7T6"/>
    <dbReference type="GeneID" id="59334142"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="141" mass="15505" checksum="A58B6FB376DC22D1" modified="2022-01-19" version="1">MAEVAKDKDVVVIIHSDRVDVGYEALKGVRIKKPSRYHSPSGVSAEGVVKLIIIAGMNNFITIPDGRHRLYYDLPQPEAAELAQTLAQKCMVDNAGIKDVGRDENDHAPFADLEKSGEVARLVQDKIDKAFGGRVKRIPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7V1</accession>
    <name>A0A8H6F7V1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147646.1">
        <property type="nucleotide sequence ID" value="XM_037297072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7V1"/>
    <dbReference type="GeneID" id="59334574"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="211" mass="23277" checksum="8D43721B817C3E7C" modified="2022-01-19" version="1">MYDSPVMQDCSQALAALPQADRFYRYYIEQQLETAPPESDWQGWTDERPSSFRMKLVQIPKFWSYGSCNLALLSYVEGNSKTALSLSRWADIYLVGYSLVQACLNLHSQGGAGTVIGTYGQPVLTMFLWQNGAIFENTINKYTSASFSAGIDPTTPAILKVFGPVDGSVDTSNGTLPEGINLSHPASLRYPNFLNNISDRLGDEPRNGGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F894</accession>
    <name>A0A8H6F894_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005750</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPF27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147838.1">
        <property type="nucleotide sequence ID" value="XM_037296657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F894"/>
    <dbReference type="GeneID" id="59334155"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008409">
        <property type="entry name" value="SPF27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296">
        <property type="entry name" value="BCAS2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SPF27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05700">
        <property type="entry name" value="BCAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="118"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="200" mass="22149" checksum="6D1776C9BBCFA1DB" modified="2022-01-19" version="1">MPLTTESYDSLPYVDRALNDTETAAAHGLIASSLSPDAESVMHPLMLDAPATSFSPLIQSELDRVAANEPLKAIDLRRYEANTTSLTPDASALKAAYVNSSYLTLRLQALELLEQFGKNAWLVANDQLESLLKEVEKELVETREATTEVNRERKRAQEGRRGELEGGERVWRAGVGGLVEVQVATVDVERRWRERLRGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8I5</accession>
    <name>A0A8H6F8I5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">glutaryl-CoA dehydrogenase (ETF)</fullName>
            <ecNumber evidence="11">1.3.8.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_006039</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>glutaryl-CoA + oxidized [electron-transfer flavoprotein] + 2 H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13389"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid metabolism; lysine degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid metabolism; tryptophan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.6" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148123.1">
        <property type="nucleotide sequence ID" value="XM_037296942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8I5"/>
    <dbReference type="GeneID" id="59334444"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004361">
        <property type="term" value="F:glutaryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01151">
        <property type="entry name" value="GCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000003">
        <property type="entry name" value="glutaryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000006">
        <property type="entry name" value="Glutaryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000008">
        <property type="entry name" value="Glutaryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052033">
        <property type="entry name" value="Glutaryl-CoA_DH_mitochondrial"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807:SF1">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0274">FAD</keyword>
    <keyword evidence="4 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="17">
        <location>
            <begin position="43"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="16">
        <location>
            <begin position="158"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="15">
        <location>
            <begin position="273"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6218688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="423" mass="46599" checksum="CB8EF61AF77EE78A" modified="2022-01-19" version="1">MIRHALRRQLVNTARTPAVTKRYAHAPVAFNWEDPLDSTSLFTEEELAIEETAYSYCQERMLPRVLDAYRDEHYDRKILEEMGELGLLGATIKGYDCAGVSSVASGLITREVERVDSGYRSGMSVQSSLVMGGIDEFGTKEQKDRFLPGMAKGKMLGCFGLTEPNHGSDPGSMETTAKPHPSKEGYLSLSGSKTWITNSPIAEVMLVWAKLQSTGKIRGFLIERDQCPPGTLETPPLKNKNGLRASITGMIQLDECPVPEANMFPEVEGLKGPFSCLNSARYGIAWGVMGALEDCIARARTYALERRQFKNNPIAKYQLVQKKLSDAVTDASYGILAATQVGRLKDEGRLAPEMISMIKRQNCDRALMNARTLQEIFGGNAVSDEYGIGRHVANLFVTQTYEGQSDIHSLILGRAITGIQAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F901</accession>
    <name>A0A8H6F901_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149177.1">
        <property type="nucleotide sequence ID" value="XM_037294489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F901"/>
    <dbReference type="GeneID" id="59331978"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034203007" evidence="1">
        <location>
            <begin position="18"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="532" mass="57113" checksum="EAD7E9C53F76EE4B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9I8</accession>
    <name>A0A8H6F9I8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Structural maintenance of chromosomes protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004088</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. SMC5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149054.1">
        <property type="nucleotide sequence ID" value="XM_037295007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9I8"/>
    <dbReference type="GeneID" id="59332497"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="6">
        <location>
            <begin position="87"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="239"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="320"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="839"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="926"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1165" mass="131211" checksum="C8ECEB68566045A4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA78</accession>
    <name>A0A8H6FA78_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fungal N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003456</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149759.1">
        <property type="nucleotide sequence ID" value="XM_037294378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA78"/>
    <dbReference type="GeneID" id="59331867"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039327">
        <property type="entry name" value="CON7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36167">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36167:SF4">
        <property type="entry name" value="FUNGAL N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="563" mass="63131" checksum="F23CC751C7987213" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAH3</accession>
    <name>A0A8H6FAH3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150163.1">
        <property type="nucleotide sequence ID" value="XM_037294085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAH3"/>
    <dbReference type="GeneID" id="59331573"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="484" mass="53923" checksum="45258FE1EDD864EE" modified="2022-01-19" version="1">MMLPEVLAGSYYSSKKDTIICTTWLSKAAFACGYQTPKVMRQAKPEPKKQESQASASRLKGEVWKEAKAAAQASKRPATDAEPYQRPKTLCRMVSEDRYLFAIGTALQPFNYISLESWKKASSARHPSGPAEPSPPKAKDAPSTSEEPSRQDLANRFGALDVEDLDESLNVAASVSPSTLSGEPRKRLSAWDRTSPNDITKSNDYTIQHCSLLTPQVQNALENLYDVLPLAIRETHYGVTSAPYQAFFKNSYYETVVHGILSRVTIGVPLRLEFGKELLVPSLVCALKPDTVIVHKAGQEFDIYDICKGNPTWALMYYPKTSNIFICPLFFLMPTLPAVGNCPTVNEATNQFEGNFTAFWQSQMYMLLHEIAHFYIGVTVEDSVDETMDWNYAFSLSPMNATSNALNYVLFVASVDKGCRDYPQADQPQSFEGRKLGTMLDLLNTSSPDIVESQRLDSVRGRVSPYHTDATNLTFAPKNETSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB52</accession>
    <name>A0A8H6FB52_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150853.1">
        <property type="nucleotide sequence ID" value="XM_037293200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB52"/>
    <dbReference type="GeneID" id="59330687"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="87" mass="9627" checksum="32A5793647558546" modified="2022-01-19" version="1">MAFLCDLANGGPNDKASKFQDKVSRNAVDYSQPQAGKDLTSCIQLKVKRNSEDKKHRNTIVPALLGIEPNTNSISPPNKYLQQQILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBI4</accession>
    <name>A0A8H6FBI4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001152</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151501.1">
        <property type="nucleotide sequence ID" value="XM_037292082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBI4"/>
    <dbReference type="GeneID" id="59329568"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050888">
        <property type="entry name" value="ZnF_C2H2-type_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24406">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR CTCFL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24666">
        <property type="entry name" value="zf-C2H2_fungi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="221"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="484"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="370"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="86"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6222066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="601" mass="66322" checksum="E875CF6D4BB4DC43" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBR2</accession>
    <name>A0A8H6FBR2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151424.1">
        <property type="nucleotide sequence ID" value="XM_037292885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBR2"/>
    <dbReference type="GeneID" id="59330371"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF5">
        <property type="entry name" value="BTB_POZ DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="46"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="239" mass="27246" checksum="FA764043BA2E264E" modified="2022-01-19" version="1">MSFSYLKFDPPGKDIPSQGYSSDFVSKAMARLVKPIDPDDSEQYPADLTIICGSLKVEAHSHVLCQDSSYFKVVCKGGFLEEQTQKLELPAEEEFLIRRLLCYRSTTGYNDESYDDEKDPPPHIVAPTYVNRLYLNAQMYSTADKYDIPSLKQKAAEKFDAAIREIHAHEMMNPYVGVSLVDEIVEAIPHIYDSTPDGDRRLRDRAAQVAICKRRDFENHPCLQDLMTAVPEFLKDIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBU6</accession>
    <name>A0A8H6FBU6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DnaJ-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001209</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151558.1">
        <property type="nucleotide sequence ID" value="XM_037292139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBU6"/>
    <dbReference type="GeneID" id="59329625"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000003">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034354542" description="DnaJ-domain-containing protein" evidence="8">
        <location>
            <begin position="31"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="34"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="10">
        <location>
            <begin position="165"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="165"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="440"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="444"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="50963" checksum="8F4CD9853F334265" modified="2022-01-19" version="1">MVRLGTVITYQTFLIRVLGFLTLTEMDSSAEDTDLYEVLEVGRGASKSEIKKAYHKAALSSHPDKVAESERASAEIKFKSVSKAYEILYDDDKRHLYDTHGMSAFDSAHGNGMGAGPDLEEMLSQMFGMGGGMPPGFSGPGPRRSRKGDDEEHPYQVTLEDLYKGKTTKFASTKNVICNHCKGSGGKEKAKAKQCASCQGKGSKQGLRSIGPGLVTQETVVCASCKGKGSVYAQKDKCKKCKGERVTEARKVLEIYIPRGSKLEPSYSYSYLLSTLLIFCREGDKITLEGEADQVPDQEPGDIIFGLMQTEHETFKRAGADLLAEIEITLVESLCGFSRVIVKHLDGRGLHIQHSQPNARVLEPGQVIKVVGEGMPHKKSELKGDLYLVVKVKFPEHGWLEDKQVLAKLKELLPTPETPIQADVVDDVAYDETANLNEFGASAEGDEGWVDDEDEEEGEAQCAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBY1</accession>
    <name>A0A8H6FBY1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000820</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152118.1">
        <property type="nucleotide sequence ID" value="XM_037291756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBY1"/>
    <dbReference type="GeneID" id="59329238"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004493">
        <property type="term" value="F:methylmalonyl-CoA epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046491">
        <property type="term" value="P:L-methylmalonyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000034">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000039">
        <property type="entry name" value="Trihydroxytoluene oxygenase (AFU_orthologue AFUA_8G02470)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051785">
        <property type="entry name" value="MMCE/EMCE_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048">
        <property type="entry name" value="METHYLMALONYL-COA EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048:SF3">
        <property type="entry name" value="METHYLMALONYL-COA EPIMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="3">
        <location>
            <begin position="208"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="364" mass="40936" checksum="7171C330FE2A5EF6" modified="2022-01-19" version="1">MSPSVITPDASSGDSEKVVLVRIAHVYYTHANFEREHRFLTDFGLTEVSRLNVGKSNETIYYRGYGSEPFLYCSTKGEEDAFGGTAFVVESRKDLELATRAIPTASEIWEMKGAPGGGECVTVKDPVDGFMVHLVYGQVQREMKEQHEQKEFNFVSAFSDTDVDGEANREASPGEQIPTDAKRSAPAHPIPDATPSNITPTGPSQVHKLGHFGMAVTDFATSFNFWTGHFNLKPTDLLHNAEGRDITTFMHLDRGQEFVDHHSFFFFEGPKPHVHHSSFEVFDFDTQVLGHDWLIKKGYENCWGVGRHVLGSQIFDYWFDTSKFILEHYVDGDQVNGETPITRDLASPDSLYIWGPDVPPRFMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC25</accession>
    <name>A0A8H6FC25_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_000973</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152268.1">
        <property type="nucleotide sequence ID" value="XM_037291906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC25"/>
    <dbReference type="GeneID" id="59329391"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000005">
        <property type="entry name" value="Sui1 translation initiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005351">
        <property type="entry name" value="ASTER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03669">
        <property type="entry name" value="ASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="SUI1" evidence="10">
        <location>
            <begin position="135"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="220" mass="24829" checksum="62DF900C02FBAAA3" modified="2022-01-19" version="1">MASRKDMRREDLIIPYKEPSAEKPDDIANTMATTLPMVAMFTRNKMIGWASFVFAVQTWLAQTPEQAKKSGTPGYMGAGMALLAVGTTYLQLFMPPPAESPRAYEFMSIENLKTFDPFAEADEDTGETKQSQNYIHIRIQQRNGRKTLTTVQGLPKKFDQKKILKVIKKKFACNGTIVNDTEMGEVIQLQGDQRKDVQEFLVDKKEGLELDAKTIKVHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC91</accession>
    <name>A0A8H6FC91_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_000634</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151934.1">
        <property type="nucleotide sequence ID" value="XM_037291572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC91"/>
    <dbReference type="GeneID" id="59329052"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1340:FF:000002">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0010">Activator</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6222588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="132" mass="15222" checksum="4E9BFA6C95B5361F" modified="2022-01-19" version="1">MADATLTNEPSDLPDTQSYPPPFKPHPSDTLYGGYTRFELELEFVTMLSSPLYLNHLASLKLLQAPDFLAYLTYLQYWSRPEYIKYLNYPGPTLRALELLQEERFRTEILSPEVVGKLMQEGVRGSVEFQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCK8</accession>
    <name>A0A8H6FCK8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152602.1">
        <property type="nucleotide sequence ID" value="XM_037291167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCK8"/>
    <dbReference type="GeneID" id="59328646"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF5">
        <property type="entry name" value="HYPOTHETICAL ACETYLTRANSFERASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="76"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="240" mass="27865" checksum="6AAB80BE206EF038" modified="2022-01-19" version="1">MPLQLAEVTSDSDFDIIIPLLWLSYESPRIPFLPLLFPVEDESAEAREKAVQRTKEIMIHMHHADPSSHWFKVTDTDTDTIIAGARWHVHEVDPYSSAPEKPFVVTSWPEGDRRKFATMNIGQVILPRMKRYRRPHLNLQVCFTHPDHRRRGAGGLIMNWGVSKADDLGVQSFIEATEVGKELYEKYGFVLVSIDTVDTNIPDPSEDWKDMERRFKANPWYCMWRPVKGNYEKGKTNLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDI2</accession>
    <name>A0A8H6FDI2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="3">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear thiol peroxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_010665</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153151.1">
        <property type="nucleotide sequence ID" value="XM_037301517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDI2"/>
    <dbReference type="GeneID" id="59339055"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22849">
        <property type="entry name" value="NuzM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000157">
        <property type="entry name" value="DOT5p Nuclear thiol peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016813">
        <property type="entry name" value="NADH_Ub_cplx-1_21kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37325">
        <property type="entry name" value="OXIDOREDUCTASE 21 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G05910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37325:SF1">
        <property type="entry name" value="OXIDOREDUCTASE 21 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G05910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="15">
        <location>
            <begin position="49"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6224091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="468" mass="49637" checksum="56464EADFFDD8CC5" modified="2022-01-19" version="1">MVELRKRKAPPDPAPSRPPMKKANSVKSITSSKKGDSSANGSAATGRKVAVGDTITIDGFGGEVETNDGEKVTLKKLVDESKSGVVLFTYPKASTPGCTTQACLFRDEFTPLTATGYSIYGLSTDSPKSNTTFKTKQNLPYVLLCDPTATLIGAIGMKKSPSGTTRGVFVVNKDGKVKAVSLGGPAATVDVVREIVGGEEVGDRVEKVAEGEKLEKEIEEGRTSAQEVGQPQTADGDEVQAEVAAEVADSAQNLDGGLVDVKAVVKAFFFASMATNAGAGAARQLQSARTATAVWKKYTVQSHGVWERIRRALAIDPERSTGIPLNPQYRNPPPGANPPEAYDDPVTVPAGDIAENAYYKRDIRRNYPRLSVVKQADVVGLLSVGSKANPKEDVLQIGDAGAKQMVQLKQEGEQKGLSAFFEKDKNVTAGIFGPDGLPPFPSGMSRASPVGGRKYVMNADREQGYPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDV2</accession>
    <name>A0A8H6FDV2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153299.1">
        <property type="nucleotide sequence ID" value="XM_037301665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDV2"/>
    <dbReference type="GeneID" id="59339204"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="27620" checksum="FE3546155B882C51" modified="2022-01-19" version="1">MSISTTLLTLPLEIRHQIYSYMLLNENIIDMIAANVTRPQEYGLFLACRQLHFEAFDYYYNANTFQLSLCDPTYSPSEYLHGDRILVERLGSMRNLHVEIGPMERKDVGNTSQDVYVLRYEREQRWRRFVKQLADVHEGQAGWLLRSLVIVARYTPRVPIQTGCLNDQPVRPPTHPSVFEKRIAALAQLVKLLEDKVGNLTIEIRSGYVWTPVVPIGYQQKPDKLFLHGIFVAEQGRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEL1</accession>
    <name>A0A8H6FEL1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4185 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153885.1">
        <property type="nucleotide sequence ID" value="XM_037301068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEL1"/>
    <dbReference type="GeneID" id="59338605"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034542258" description="DUF4185 domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="57974" checksum="D10B4695617149CC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF44</accession>
    <name>A0A8H6FF44_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Alkaline phosphatase</fullName>
            <ecNumber evidence="3 15">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_009043</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="3 15"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154730.1">
        <property type="nucleotide sequence ID" value="XM_037299904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF44"/>
    <dbReference type="GeneID" id="59337438"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.40:FF:000002">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000063">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF5">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 13 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="12">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6226177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="595" mass="65488" checksum="2A8BD33861657F58" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF97</accession>
    <name>A0A8H6FF97_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7514 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009208</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154895.1">
        <property type="nucleotide sequence ID" value="XM_037300069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF97"/>
    <dbReference type="GeneID" id="59337603"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055936">
        <property type="entry name" value="DUF7514"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39611:SF2">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN DZ-HRGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39611">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN DZ-HRGP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24355">
        <property type="entry name" value="DUF7514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7514" evidence="2">
        <location>
            <begin position="13"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="460" mass="51362" checksum="07C412102E749B8C" modified="2022-01-19" version="1">MAGTAVAAQEYWGYLIQPDKSPTPVFEQLLLGVANYINRHIAPWDIQTLTPAKLAAFYRLVGGDYDPLFLETSQTSLSFIYQSLGCYHTLQPEKDPYTPPTIPSLTPAGFVRWQTVQLLLEPEEHVPFLQEAVKRFDLTNPADGAPFPSILPKESLPCQPDIEMVEWHETVAEKLMLEAQASAARAMPPRPNLALSDIDLESSRDTSSVDSYSGVDAAHGAGYFSHPRANNRGPLFVQVPYRVRPPPQYPNQEPWSPERRRSSLPDDRAYPSSWPRDGPTPTNYAPPPPLPPPNSHPQHHLTHPHHHPHVRPRHTRIPSDISITSSDTSDTSSMTTSSASMSPVRYHAKLHSPSGPSGPRRHSSTVPPQRPAQGYSPYQRQPSSPSPSKAKNVRWQDMDDVFDAPRHNIPRNRSMDLRGRGDPRNEYRVEDQRRGARSVGPVTGVGGRRYAPNGSAMDWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFQ7</accession>
    <name>A0A8H6FFQ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Folylpolyglutamate synthase</fullName>
            <ecNumber evidence="17">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_008293</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </cofactor>
        <text evidence="17">A monovalent cation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 17">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="17"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155161.1">
        <property type="nucleotide sequence ID" value="XM_037299160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFQ7"/>
    <dbReference type="GeneID" id="59336690"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000009">
        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023600">
        <property type="entry name" value="Folylpolyglutamate_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF5">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038895">
        <property type="entry name" value="FPGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="13 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 17" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="19">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6226852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="501" mass="55396" checksum="63B35C5B67F1A29E" modified="2022-01-19" version="1">MASRTYADAVAALNTLQTNFAIVDAIRNSGKGMNKNSIPEMVEWCRRIGYEPSDFNRLNAIHIAGTKGKGSTSAFISSMLGQYRFPSTRKPERPLLRKIGLYTSPHLRYVRERIQINNQPLSEEAFARYFFETWDRLEEAARAKGEPPDTTAKPGYFRFLTLMAYHTYMSEGVDTAITECGIGGEYDSTNVLVKPTVTGITSLGIDHTAMLGNTVEEIAWHKGGIMKASAPCFTAPQPPAAIEVLQKRADEVPTKLHVVGRNPLLEDVKLGLAADFQKINASLAVAIADAHLVALGQENRKRQDDMLPPEFIRGLEEVRWPGRCETRREKNIAWHIDGGHTLESIEVAGSWFASQIPPARSRKRTRILLFNQQTRDANALAKALHRTLASALQDEQPFTHAVFCTNMTFREEGYRPDLMSMNTSAAAAEKLDVQRGLAETWRGVDPQTIVEVKLSIEEAVGWCKDVAGVEKGDAEGEVMVLVTGSVHLVGGFLEVLETGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGF2</accession>
    <name>A0A8H6FGF2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155233.1">
        <property type="nucleotide sequence ID" value="XM_037299232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGF2"/>
    <dbReference type="GeneID" id="59336762"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034219291" evidence="1">
        <location>
            <begin position="21"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="203" mass="20152" checksum="BAD3F64E4D30A95E" modified="2022-01-19" version="1">MSSTLFFLLGLTALIPQALAISIPANPAVGLTNIISVDTPAIILTGPFGTFIRSPADALDCLAAAQTAIAATIAAVGDLPIPHPDWHFSSHNVQLIASAPFPTLGTYGQLSSALRGLLYLITRAGGPGARQLGWVLSDGAQGLGIVMRGSMAPRTITIVGAAPAGPTAGGAMGGGTVASGAAMATATATQSVSIVGVKELDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGJ9</accession>
    <name>A0A8H6FGJ9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008529</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155396.1">
        <property type="nucleotide sequence ID" value="XM_037299395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGJ9"/>
    <dbReference type="GeneID" id="59336925"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="84" mass="9183" checksum="B9B7E09DDD359CA3" modified="2022-01-19" version="1">MPSSQKIRPVQGTADARTKFTQDELSKTVDFCFQAFPHAPAEKRDAVLEKMKGITSDGRGSADEETKAAEKVLESALSADEIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH96</accession>
    <name>A0A8H6FH96_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156164.1">
        <property type="nucleotide sequence ID" value="XM_037298828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH96"/>
    <dbReference type="GeneID" id="59336357"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36142:SF5">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36142">
        <property type="entry name" value="METALLO-HYDROLASE/OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="402" mass="44039" checksum="C8FA053B3CB88802" modified="2022-01-19" version="1">MSLTVKHLNGDTTFLLTFSPNDNYPQTPLGSQFQQPPGTFSILVDPWLSGPTTMWHPKFLLSKHTTPSCIPHLSHIPEPNVVLISQDKPDHCHEATLRQLDPTSPITTILAEPTAAKKIRGMKFFHPDMVHSLRPFSERKPDSVIRFYIPPIIPGGTPGEATISFIPAKLDMAKVHNAIGVTYRPPSSSCIPSRPFSPISPNSQPPFHQSQYPPAPQNLPMTPPESPTWQSSTLRTDSNTLSSASTPSSHQSNSNHNLSISSRSSVTSTSRIHAEKALSFIYSPHGVNYSLIRAYASTHLVSIAALPLTLLLHSFDRVENPWYLGGNVAAGSPGGIEIAQNLMARCWISAHDEDKDNTGLSVMSVKTRKYTKEEVRKMLRQELGSNVNVANLAPGAEMVLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FID5</accession>
    <name>A0A8H6FID5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MoaB/Mog domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007202</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>adenylyl-molybdopterin + molybdate = Mo-molybdopterin + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>molybdopterin + ATP + H(+) = adenylyl-molybdopterin + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:31331"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58698"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MoeA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the MoeA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the MoaB/Mog family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156730.1">
        <property type="nucleotide sequence ID" value="XM_037298095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FID5"/>
    <dbReference type="GeneID" id="59335602"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061598">
        <property type="term" value="F:molybdopterin adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000002">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000011">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000013">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045515">
        <property type="entry name" value="Glp_gephyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF5">
        <property type="entry name" value="GEPHYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="4 5" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="7">
        <location>
            <begin position="9"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="MoaB/Mog" evidence="7">
        <location>
            <begin position="431"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="180"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6229088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="673" mass="71614" checksum="2D7A540582BF6710" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIJ3</accession>
    <name>A0A8H6FIJ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial division protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_007309</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in mitochondrial fission. Acts as an adapter protein required to form mitochondrial fission complexes. Formation of these complexes is required to promote constriction and fission of the mitochondrial compartment at a late step in mitochondrial division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the WD repeat MDV1/CAF4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156835.1">
        <property type="nucleotide sequence ID" value="XM_037298200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIJ3"/>
    <dbReference type="GeneID" id="59335708"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22881">
        <property type="entry name" value="Mdv1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000881">
        <property type="entry name" value="Mitochondrial division protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053299">
        <property type="entry name" value="ASTRA_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44156">
        <property type="entry name" value="SUPERNUMERARY LIMBS, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="315"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="357"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="433"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="540"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="394"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6229193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="651" mass="72674" checksum="DEAEFF18668C6623" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ02</accession>
    <name>A0A8H6FJ02_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CHAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157004.1">
        <property type="nucleotide sequence ID" value="XM_037298369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ02"/>
    <dbReference type="GeneID" id="59335877"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024983">
        <property type="entry name" value="CHAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12770">
        <property type="entry name" value="CHAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHAT" evidence="2">
        <location>
            <begin position="457"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="729" mass="80967" checksum="1A6EB17625809486" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJV7</accession>
    <name>A0A8H6FJV7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 8 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004237</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157157.1">
        <property type="nucleotide sequence ID" value="XM_037295156.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332646"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059070">
        <property type="entry name" value="TPR_VPS8_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25066">
        <property type="entry name" value="TPR_VPS8_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 8 central" evidence="2">
        <location>
            <begin position="709"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="domain" description="VPS8-like TPR-like repeats" evidence="3">
        <location>
            <begin position="1249"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="864"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1485"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1559"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="874"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1485"/>
            <end position="1516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6229900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1580" mass="174140" checksum="BD22B553263F6AC8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK73</accession>
    <name>A0A8H6FK73_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004384</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157303.1">
        <property type="nucleotide sequence ID" value="XM_037295302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK73"/>
    <dbReference type="GeneID" id="59332792"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034534338" evidence="2">
        <location>
            <begin position="30"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="217" mass="24038" checksum="2C50E575D59ABF01" modified="2022-01-19" version="1">MLVSSPKYLGTFLTLFTLILLFLTHRHAAENYLGQREMEYADETSDQTVIGGGMTELQETVAYDKLSGDTPSDIEANWSATIGGKRLLQKVQDFRHSTQASYNDASKESVRQTLEDAELELETLGRESFKTKKIVQGVRKLEVALKAAEEAIARGPEAKTMTSKSGEGDTADTQLEEAFQEAEADRKDKENTASLEKLQKAELDAVIAESKESPDFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL48</accession>
    <name>A0A8H6FL48_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004854</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157767.1">
        <property type="nucleotide sequence ID" value="XM_037295766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL48"/>
    <dbReference type="GeneID" id="59333260"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF69">
        <property type="entry name" value="PEROXISOMAL LONG-CHAIN FATTY ACID IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="123"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="461"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="682"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6230510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="715" mass="80507" checksum="BC2E258CC930C3C4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FLJ2</accession>
    <name>A0A8H6FLJ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157825.1">
        <property type="nucleotide sequence ID" value="XM_037290951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FLJ2"/>
    <dbReference type="GeneID" id="59328430"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="560" mass="63630" checksum="5FB1EC039E3ACADC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY68</accession>
    <name>A0A8H6KY68_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156646.1">
        <property type="nucleotide sequence ID" value="XM_037298011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY68"/>
    <dbReference type="GeneID" id="59335518"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="254" mass="29794" checksum="953C95A70EE26324" modified="2022-01-19" version="1">MPSRNYAPKISYNSREINKIHNLLNTHSKALTNHLLDFSPILLIDTIISNFAIKILHNVQPLTYKIYIEITVFPLNKTTILIIRRILFVFNKQSEIKSFGLEDTRILYEHNTILKLYDDIIDIVTTSEDEDDEDEDDADKDDEDKDNEDKDDADKDDEDKDNEDEDEDEDDGIRHEQLLNALEDVYSWVQSESRLAFSERLAFDRNTHIYSVISTAIRYGDHKAVENLIYSEINRRCNLFLRYRYGEDPENAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYA0</accession>
    <name>A0A8H6KYA0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156690.1">
        <property type="nucleotide sequence ID" value="XM_037298055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYA0"/>
    <dbReference type="GeneID" id="59335562"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="492" mass="56125" checksum="3A7DF1C0DA160A55" modified="2022-01-19" version="1">MDADQPIQLTPKQSHNSAEAIDTQSTSSSDLSSHEYCKQLLLNLTPYVLIPATPARQTSEFFDSINNLDPVKFSPPDSNQTHLKKAMSDASFAKLNPFYDERYTQMEDFYDSQAWKVGTGTGKSPQPPPKYGHHKGHKSPGMAHSQSEGNIDFRSRGTKDHNLRVGKTFEGAAGDYYTRSSEDTDASSSFSRGRTITRTPSPVKALEDIKEDQASNSPPSPTKRSRSPFKQMFGEKGWLGRSTSMNELPNEKYRKTGIKHWGGKIKQRVENLTEDVSKVSKFIPTTLYQHESPSKSPSKTKFYVSLSPPMQAKLYSEMELMICATANQYLNVQQKEGRMTVESLKKVTEWWASKNRPQVIEFMFDQATQRDLVLYNLKSFRFYGPNADNSFQMNAMMQSWKALSRDMSVRTFCTPDSVIRKHTHDCYKILEMLGAPLVTFMAFQEIQMKALKVMREEQKKRDEYEAIKFGVERKWEAPTEGAEEKELYNPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYP8</accession>
    <name>A0A8H6KYP8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_007288</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156814.1">
        <property type="nucleotide sequence ID" value="XM_037298179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYP8"/>
    <dbReference type="GeneID" id="59335687"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1070">
        <property type="entry name" value="Translation initiation factor eIF-2B, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042528">
        <property type="entry name" value="elF-2B_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="301"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6229172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="352" mass="37347" checksum="1A3D38B532617387" modified="2022-01-19" version="1">MLTPPPEENTAIPPSSDPNNGSPSFNLVSTYKNILSTDLLITKPIAAIESLISLLSASPPSTISETLSLLESATSELKDSVPNSIALSAGTDLFQRYLISTLQQGSAQSAKSKGKRSSSGGDGDFRAIRAHLLANGRLFAQRANESRGQIAKVARKFVRDGCTVLTCGRSRVVAAVLGAAAEDGAKFRVVYAQDSNDGLSKNSADIVTELWRKDIPVATITFAALAASLPYVTFALVGAESVVENGGIVSGMGTNQMGLLAKSVGKPFYVVAESHKFVRVHPLSGDDLPLGQKVLDFSTQSREEEKEHEEGKRKAGDEVVDFTPPNLITAIVTESGVQTPSAVSEELIKIWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYQ0</accession>
    <name>A0A8H6KYQ0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156888.1">
        <property type="nucleotide sequence ID" value="XM_037298253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYQ0"/>
    <dbReference type="GeneID" id="59335761"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="189" mass="21197" checksum="64CC986ABA5C9319" modified="2022-01-19" version="1">MNSYAARLASFTSWPHIYPSASTVARAGFHREFDPTFPSPDLTQKKKHRNQKTRQQYTRRSLQPVTQSATQQATQSSTQSATQQATQSDTLQEEELQQANAENTAENTAEKSSGSKAFSTKNTGLLFKIPRYMLSTIPRQITTQQAENDTTTWPVIPTALIGYITAMTSQEEDLLRRSQPIGPLQEEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYR8</accession>
    <name>A0A8H6KYR8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156976.1">
        <property type="nucleotide sequence ID" value="XM_037298341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYR8"/>
    <dbReference type="GeneID" id="59335849"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="217" mass="24548" checksum="25DDC91D72BEC1FB" modified="2022-01-19" version="1">MFDEFCQREDKYGLEQYSTQTEKEYLAWLEELAQNIKCNVEIIYGTAGKCSGGLGRIVVFAKHPETFCRDWRPSTAILQDRYCTAAAKLNCVENFPNFYQDRVRLEKVRIGDLAIDMLLCERMGNSVTFEDLSPHYQKFMLRRFSVRDYGELMIVYADVFGLTEDTPGDDYPGIASMAHSLVSRVATYKNAERGNNSSDDGGKELVSSLGSCASMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYW3</accession>
    <name>A0A8H6KYW3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157035.1">
        <property type="nucleotide sequence ID" value="XM_037298400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYW3"/>
    <dbReference type="GeneID" id="59335908"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="364"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="407"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="525"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="638"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="935"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1055"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1091"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1171"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1219"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="246"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="485"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="541"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="586"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="640"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="742"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="770"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1055"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1239"/>
            <end position="1250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1250" mass="136982" checksum="B4804046B967ED17" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZG2</accession>
    <name>A0A8H6KZG2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Endoplasmic oxidoreductin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_004241</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May function both as a monomer and a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157161.1">
        <property type="nucleotide sequence ID" value="XM_037295160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZG2"/>
    <dbReference type="GeneID" id="59332650"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF0">
        <property type="entry name" value="ERO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017205">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034501113" description="Endoplasmic oxidoreductin" evidence="20">
        <location>
            <begin position="22"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="478"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="478"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="16">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="100"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="402"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6229904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="65663" checksum="63822A8FE815FC07" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZK2</accession>
    <name>A0A8H6KZK2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="8">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004362</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Ubiquitin thioesterase component of the regulatory network controlling carbon source utilization through ubiquitination and deubiquitination involving creA, creB, creC, creD and acrB. Deubiquitinates the creA catabolic repressor and the quinate permease qutD. Also plays a role in response to carbon starvation and the control of extracellular proteases activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with creA, creC and qutD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157281.1">
        <property type="nucleotide sequence ID" value="XM_037295280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZK2"/>
    <dbReference type="GeneID" id="59332770"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02663">
        <property type="entry name" value="Peptidase_C19G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000075">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase creB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF733">
        <property type="entry name" value="RE52890P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="57"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="94"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="528"/>
            <end position="725"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
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            <end position="104"/>
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    </feature>
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            <begin position="147"/>
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        <location>
            <begin position="664"/>
            <end position="674"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="715"/>
            <end position="725"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6230024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="725" mass="80671" checksum="77C0CC810831A42F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L091</accession>
    <name>A0A8H6L091_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037290959.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="343" mass="38803" checksum="C32981ABCE2D13AA" modified="2022-01-19" version="1">MADQDSSSFFGGITLTSTPQSTFAGLRSHPSQRDSSASTSRSSIDNQIREDVLVNNTSNGMRWLFMAYKYRLLWGVALMLAVTPLLLRFALQPGPSNNARHPPSPTVDTCPRLASVINLSPVHVFDRSVYMSTVDEHTLLEMEKSWSDITLTVMDTSRPLGCLAYASEKSTRLSNQDQDKYHVFLDDVIAVQSQLEYTSLNISHLMDHYRLLRPQYHEHNSSAARALEVSERSGFKKGLIAGKFVTWAYLGVNFTKWYRDPWVKAHKTFKAAQLGVATFEGEMSKLESIAQVIQEVNHHLDRLAFVVFEDVVKGDCYSCMVRTRDILMLVRQLWGRLLAMGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C515</accession>
    <name>A0A8H6C515_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">HO133_006662</name>
    </gene>
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        <name type="scientific">Letharia lupina</name>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>Lecanorineae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037297559.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000593566">
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        <property type="entry name" value="DH_2_fung"/>
    </dbReference>
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        <property type="entry name" value="PH_23"/>
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        <property type="entry name" value="PH_24"/>
    </dbReference>
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        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24344">
        <property type="entry name" value="PH_23"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
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            <end position="982"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
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            <begin position="1404"/>
            <end position="1562"/>
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            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
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            <end position="306"/>
        </location>
    </feature>
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        <location>
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            <end position="365"/>
        </location>
    </feature>
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            <begin position="383"/>
            <end position="521"/>
        </location>
    </feature>
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            <end position="849"/>
        </location>
    </feature>
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        <location>
            <begin position="990"/>
            <end position="1054"/>
        </location>
    </feature>
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        <location>
            <begin position="1086"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1644"/>
            <end position="1666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
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        <location>
            <begin position="422"/>
            <end position="436"/>
        </location>
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        <location>
            <begin position="485"/>
            <end position="496"/>
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        <location>
            <begin position="827"/>
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        </location>
    </feature>
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        <location>
            <begin position="1006"/>
            <end position="1017"/>
        </location>
    </feature>
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        <location>
            <begin position="1126"/>
            <end position="1138"/>
        </location>
    </feature>
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        <location>
            <begin position="1155"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1320"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1346"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1380"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1645"/>
            <end position="1661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6217560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1814" mass="196933" checksum="F5375DF4C291FFEA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C536</accession>
    <name>A0A8H6C536_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146907.1">
        <property type="nucleotide sequence ID" value="XM_037297706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C536"/>
    <dbReference type="GeneID" id="59335210"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="217" mass="23049" checksum="0B7E378871077B58" modified="2022-01-19" version="1">MGSSPSKPPRKISRPIPQNPTPTFLPYPSLAPLPRRPAPSRPSPEEDLAGRYGRILAANPVYAGARREEPGPGPRAPFRFAPLSEIGAKAQPGVGVGGEMKKEKKKKKNGYGVFPAAEKGDYRGGAGGGAGAAPPTTTQHQHRQGQGQAQAQAQAQAQAQAQAQAQAHGQENAAAKAKKGARFGGTRQDRHGEWWVLVLADGRWYRSKEMGLRGPGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5V3</accession>
    <name>A0A8H6C5V3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147035.1">
        <property type="nucleotide sequence ID" value="XM_037297599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5V3"/>
    <dbReference type="GeneID" id="59335103"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008313">
        <property type="entry name" value="GH125"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047:SF1">
        <property type="entry name" value="DUF1237 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 157 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06824">
        <property type="entry name" value="Glyco_hydro_125"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028846">
        <property type="entry name" value="UCP028846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01149">
        <property type="entry name" value="DUF1237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034262225" evidence="1">
        <location>
            <begin position="20"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="469" mass="50791" checksum="295A1AD2F83889B3" modified="2022-01-19" version="1">MRPVILLFQWLCTSTLAAAQTCPDYADYAAAPHAPFSGGVYNLSYQRPAPSCRTFTSPAVESTIARLNSTITDPDLSRLFENSFPNTLDTAIKWKGYAADNADEELTFVITGDMYTPPSESGLPPSINSAATNDVVTPSYANTTVYECKYELDSLAAFLEISTDYYTATNDLAFFSKYSWVAAVQAVLNTAQSMTISTYAANGSVNTLPYTFQRLTTSAEDTLANKGTGNPVQNGTDLIRSAFRPSDDACIYQFFIPANMMFAHQLNTTSALLTAMGTANSTDLATELTALSASLSAAITRHGIFPDPVHGAVYAYEVDGYGGRNIMDDANIPSLLSAPLIGYLAQNDSVYQATRALLLSADTDPYFMRGPVIDAVGGPHDGPGFAWPMASIVRIFTSGDDGELAGSLKELVGSTDGLGGWSACDVFPFIFFFFFCERLYKTDQPTFHPTGLIHESVNTFDQSHYTRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6R2</accession>
    <name>A0A8H6C6R2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_006422</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147445.1">
        <property type="nucleotide sequence ID" value="XM_037297320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6R2"/>
    <dbReference type="GeneID" id="59334823"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000180">
        <property type="entry name" value="MFS monosaccharide transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF61">
        <property type="entry name" value="HIGH AFFINITY GLUCOSE TRANSPORTER RGT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="404"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="449"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="477"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="31"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6218010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="557" mass="60051" checksum="F90E2B7C6499A5EE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C784</accession>
    <name>A0A8H6C784_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptide transporter PTR2-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_006227</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147700.1">
        <property type="nucleotide sequence ID" value="XM_037297126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C784"/>
    <dbReference type="GeneID" id="59334628"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000085">
        <property type="entry name" value="MFS peptide transporter Ptr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="60050" checksum="525177EED6ACE71A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C802</accession>
    <name>A0A8H6C802_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3beta-hydroxysteroid 3-dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.270</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005928</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.270" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148012.1">
        <property type="nucleotide sequence ID" value="XM_037296831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C802"/>
    <dbReference type="GeneID" id="59334333"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000253">
        <property type="term" value="F:3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051593">
        <property type="entry name" value="Ergosterol_Biosynth_ERG27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647">
        <property type="entry name" value="DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647:SF4">
        <property type="entry name" value="KETOREDUCTASE (KR) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="315" mass="34215" checksum="9E4E3905C5865957" modified="2022-01-19" version="1">MKGTFVITGANWGLGSNLVSQFLRSSYAKDYKGLFAVRIPASADTLKKVLAGSSNANDHEIIAVDLSTLASVRAATKDLNQRVASGSIPHIRAVVLNAALQHNMLYSRHESTDDGLEASFAVNYLSHFLFTNLLLQSMDPKGRIVVVSSWTHDPSYYVNSGHITNEQHKTVIPEGGPQVLAKPPSKAAKGDEYKAGMRRYGMSKTLMVMFMYELQRRLAGTKYSDISVLAVDPGAMGDSGLFKESPAALRFVLHWMLAPLAVVTTILSPNGNFRTPKKSAKDLLNSSFEEGTLGKHPKAAYLNGSAKMKPSKEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8B4</accession>
    <name>A0A8H6C8B4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ceramide glucosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.80</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glucosylceramide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">UDP-glucose ceramide glucosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">UDP-glucose:N-acylsphingosine D-glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_005365</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.80" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148257.1">
        <property type="nucleotide sequence ID" value="XM_037296275.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333771"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006679">
        <property type="term" value="P:glucosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048800">
        <property type="entry name" value="Cac1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022043">
        <property type="entry name" value="CAF1A_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726:SF0">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21796">
        <property type="entry name" value="Cac1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12253">
        <property type="entry name" value="CAF1A_dimeriz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13506">
        <property type="entry name" value="Glyco_transf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034152748" description="Ceramide glucosyltransferase" evidence="16">
        <location>
            <begin position="20"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 subunit A dimerization" evidence="17">
        <location>
            <begin position="607"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 subunit Cac1-like C-terminal" evidence="18">
        <location>
            <begin position="842"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="252"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="297"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="374"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="447"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="645"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="374"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="448"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="471"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="649"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF21796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6218822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1361" mass="152800" checksum="57047A1B048E7DED" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8C0</accession>
    <name>A0A8H6C8C0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha-methylacyl-CoA racemase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoA-transferase III family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148268.1">
        <property type="nucleotide sequence ID" value="XM_037296286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8C0"/>
    <dbReference type="GeneID" id="59333782"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="entry name" value="formyl-coa transferase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050509">
        <property type="entry name" value="CoA-transferase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228:SF5">
        <property type="entry name" value="ALPHA-METHYLACYL-COA RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228">
        <property type="entry name" value="SUCCINYL-COA--D-CITRAMALATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="41620" checksum="3FB4F7AB65B84EB0" modified="2022-01-19" version="1">MATSPPPLTGLRVIELAGLAPGPFACLLLADYGASVLRIDRAHPQAHTLNPPPPTSDSLSRHKSSIALDLKSPNGLALLRSLLNHADILIDPFRPGVLESIGLHPKDLLIVNPRLIIARLTGFRRDGPYAPMAGHDINYLAVSGLLSQLGRANAPPSFPANIIADFAGGGLMCAFGILAALVSRTQTGKGQIVEANMVDGSAYLGTFSRFLTKTPLGDRPRGENMLDGGCPWYEVYECKNGGYMAVGALEDSFFGELLKGLNIEQELMTTRNDRTTWPTLRKTFRERFLENTRQEWELLFAGKDACCTPVLSQIELEEQGYEQRSAVGLMGSPGLPIPQTEAWSSPNLSPGAGGEEVLKGWTGWKRGKEYQVENGGLVKTEASKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Q1</accession>
    <name>A0A8H6C8Q1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AA9 family lytic polysaccharide monooxygenase</fullName>
            <ecNumber evidence="1">1.14.99.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endo-beta-1,4-glucanase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycosyl hydrolase 61 family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005251</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Lytic polysaccharide monooxygenase (LMPO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and/or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1-&gt;4)-beta-D-glucosyl]n-1 + [(1-&gt;4)-beta-D-glucosyl]m + acceptor + H2O.</text>
            <dbReference type="EC" id="1.14.99.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM).</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.56" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148144.1">
        <property type="nucleotide sequence ID" value="XM_037296162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8Q1"/>
    <dbReference type="GeneID" id="59333657"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182:SF2">
        <property type="entry name" value="LYTIC POLYSACCHARIDE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034981497" description="AA9 family lytic polysaccharide monooxygenase" evidence="3">
        <location>
            <begin position="18"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Auxiliary Activity family 9 catalytic" evidence="4">
        <location>
            <begin position="93"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="329"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="417" mass="41014" checksum="C60BC1AE6F9407E9" modified="2022-01-19" version="1">MSILSFSSALFAATTSAHMILGSPPPYGSPDNSPLVADGSNFPCKATSNAGAIVTEMAIGAPQPLWFIGSAVHGGGSCQISLTTDMPATKSSSWMVIHSIEGGCPAKGVSGNDAAGQSGGSNYTYTIPQGITPGSYTLAWTWFNKIGNREMYMNCASIKVSGGSKKRAATLNETLGIPELYERTTPSFPGMFKANIPTTDCETVDSADVLFPDPGASVEKDGTSALTAPTGAKCNVVAGGGSSSSNSSTAAAGSSSSGSSGSGSSGSGLSGSGSSAPASPGSSSAASAAPSMATSDAPAPAATSMAAAPSVSASPVVAAAPSVAAAAPSSAPSAITNSSSSSTTPSTSSTGTCSTAGQSVCSPDGKQIGECTPQLTVTWIPVADGTKCVGGYQVFAKRSNPKFQKGYVRRGEGLGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Q4</accession>
    <name>A0A8H6C8Q4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_005463</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148355.1">
        <property type="nucleotide sequence ID" value="XM_037296373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8Q4"/>
    <dbReference type="GeneID" id="59333869"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008024">
        <property type="term" value="C:cyclin/CDK positive transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07840">
        <property type="entry name" value="STKc_CDK9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000440">
        <property type="entry name" value="Serine/threonine-protein kinase bur1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000270">
        <property type="entry name" value="Serine/threonine-protein kinase bur1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF546">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="384"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="695"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="300"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="695"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6218920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="746" mass="84911" checksum="7AB1D994E2A0B27B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C920</accession>
    <name>A0A8H6C920_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Haloacid dehalogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily. S-2-haloalkanoic acid dehalogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148582.1">
        <property type="nucleotide sequence ID" value="XM_037295884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C920"/>
    <dbReference type="GeneID" id="59333378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019120">
        <property type="term" value="F:hydrolase activity, acting on acid halide bonds, in C-halide compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006328">
        <property type="entry name" value="2-HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051540">
        <property type="entry name" value="S-2-haloacid_dehalogenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01493">
        <property type="entry name" value="HAD-SF-IA-v2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01428">
        <property type="entry name" value="HAD_type_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316:SF3">
        <property type="entry name" value="HALOACID DEHALOGENASE, TYPE II (AFU_ORTHOLOGUE AFUA_2G07750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316">
        <property type="entry name" value="HYDROLASE, HALOACID DELAHOGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="26701" checksum="63A2AB0FF06D6E4B" modified="2022-01-19" version="1">MAVNTVIAFDLYGTLLSTESIAKELAAHFGQEKAASLAGLWRRYQLEYTWRLNSMNQYQPFSDITRNSLDHALADFDLSLDDKSTSQLMKAYDSLSTFPDVSPALKALADETGITAVVFSNGTNSMVTNSVQSSPDLGPHASVFKEIVTVEQVKCFKPDPRVYHHLAEKMGKGRTKEEMGQMWLVSGNPFDVVGARAVGMQAAWVDRGGAGWTDGLVRGETGRPTAVVKGLGEVVEAVKEYCRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C997</accession>
    <name>A0A8H6C997_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005112</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148722.1">
        <property type="nucleotide sequence ID" value="XM_037296024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C997"/>
    <dbReference type="GeneID" id="59333518"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051232">
        <property type="entry name" value="ARID/SWI1_ChromRemod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13964:SF27">
        <property type="entry name" value="HAT-TRICK, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13964">
        <property type="entry name" value="RBP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ARID" evidence="5">
        <location>
            <begin position="327"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="91"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1041" mass="114853" checksum="71BE454128D8322F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9C3</accession>
    <name>A0A8H6C9C3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148808.1">
        <property type="nucleotide sequence ID" value="XM_037296110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9C3"/>
    <dbReference type="GeneID" id="59333605"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="428" mass="46866" checksum="914FA05D40D2AE4C" modified="2022-01-19" version="1">MPPQRTIPPAKALKTERTHEENQERAYIAASRRSDRSLEARVESARRASEIHKRRTGRSLRVTEQDVVNEEMYEEEDDDLPMQYRRLTAHLQTGSADFNRRLSAYLTNHVAMRSALDQAITNSYAQQYPNAPQFAHNQNIYPSPFMAHKMPQQQSPSFGQPYPTPGTPGYRPSHQARSASGSHGTPTYSPNMPAPSPIQAVPQMDRRRTSASSKSSSHSPTGQPVTPQSMHAMPALPQTSSTSNVKQEGQTSQQMRAPQQPSPVQRQHSFGAMNGFSNMSPFTTSLPTEAQMLLGPALDPNDSFTSMLMAGSEALPQSYNYNTTPSTFQKPRSFNQSYDGMSATLAPSALDMSPRHQIYAQPSTTISASPASSPAFQLPYDASLHDFSKCQIYAGGNSASGSGTVTPAGIDGGWDAFINDTSWTENTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9E7</accession>
    <name>A0A8H6C9E7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005229</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148838.1">
        <property type="nucleotide sequence ID" value="XM_037296140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9E7"/>
    <dbReference type="GeneID" id="59333635"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039193">
        <property type="entry name" value="Ribosomal_uS17m_metazoa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24088">
        <property type="entry name" value="28S RIBOSOMAL PROTEIN S17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24088:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="133"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19028" checksum="9C5E90B7BA2028CF" modified="2022-01-19" version="1">MSPMAAALSLTLTYRHPRHTIIHLAYTAMFGPPPKVLCGVVVSAGKMMKAVKVRTAKQTYNKFLKKHFRSNNSYLVSDPNSSLRAGDVVKIAAERRISRHIKHVVTEIVAPWGPSIDERPPVLSEEERLRRLQEKTLRKKERRRERNAETGESAEDRVEGMVEGTAVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9F4</accession>
    <name>A0A8H6C9F4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Membrane fusion mating protein FIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148775.1">
        <property type="nucleotide sequence ID" value="XM_037296077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9F4"/>
    <dbReference type="GeneID" id="59333571"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033481">
        <property type="entry name" value="Dni1/Fig1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016509">
        <property type="entry name" value="Fig1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092:SF1">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12351">
        <property type="entry name" value="Fig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007138">
        <property type="entry name" value="FIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="28905" checksum="7856D7541261114D" modified="2022-01-19" version="1">MANMRFVMSFRRALPFLGYHHVLMILIAIAIILLSLLLAGCSSSSPLIPSIFLISLYYKSYPPSFDPSQVDPGVTTAIANIVGQAQLEVRVGYFGICINPDGGSFLCSNNASSLATQVSVDQDPLNLIWVANTFKDSIVFPYLIIVAIVLAFVCFLLLATFPGWHEEHDDNGSDREVKPFPSRPVSQVALALIFVASVFVLVSVLWQHTASVGASTIAQDLGNGSVKSGVGTAAMILGWFGFALLIIVTIGLLVMILSIRILDQLTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9G2</accession>
    <name>A0A8H6C9G2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148476.1">
        <property type="nucleotide sequence ID" value="XM_037296494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9G2"/>
    <dbReference type="GeneID" id="59333991"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="282" mass="32530" checksum="4467BF6D615DE002" modified="2022-01-19" version="1">MKIPLLDAFCSSFLSITNSLLPNPPFSNFQFTQKPEHNMAPGQLPRPTRQPNHPPFPDPTQHKGQERVHRPSSEAYQAFRLRMDMQHHSWLQRTKDHFHQLIMWAERQPSNGTVRKVERPNDEDRFAVAYGTGPDPDLRDLLEKIFAKHGEVSLQHDGRDSTGKHWIKCFGKMQPGSLERPTEKAIVCVSSKLEMATIPRPRTRVVPGKGKEKATRGGNEWPPQVPGVVKGAPENLFSLPWFKQLPWLEPGVAEVLREKMEAEEFEFEAERWEQLARRRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9P4</accession>
    <name>A0A8H6C9P4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004084</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149050.1">
        <property type="nucleotide sequence ID" value="XM_037295003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9P4"/>
    <dbReference type="GeneID" id="59332493"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF234">
        <property type="entry name" value="MFS-TYPE TRANSPORTER DBAD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="451" mass="49119" checksum="5B6468425E528D43" modified="2022-01-19" version="1">MPPYAGKVLAARKESAVSTAYGAPSAEGMVAIPATGYDDPPDDGLVAWLQVAGSFFLFFNSWGTVNAFGVFQTYYHIGFLSDKSPSNISWIGSIQSFLLLVIGVITGPLYDAGYFRALILTGTSLIVLGFMMTSLCTQYWQVILAQAICIGLGQGCLFIPSVAIIPQYFTNKKAFATGLASSGSSLGGVIYPIVFSQLQPRIGFSWATRVLGFISLTTCLVSISVMRVRQIPKQRRSLVEFAAFKETPYSLFCLAMFFCYAGAFGPIYYISSYAIDTRVMTARLAFYLLPILNAASVPGRIITGFLANTMGPVNLLIPTALTIGIIALCWIGIHSTGGLIVFAILYGFFSGGVVSLPSVGDEDGNVLCALQSGKSLRYTDLRRDSGWDWGLGRGGIVFWVYTVSYWSAFSLHTMGSGRDEFEDEGLRKAKERWTVRRARFEKVRAPNAQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9U5</accession>
    <name>A0A8H6C9U5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_005134</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4 12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the histone H2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148744.1">
        <property type="nucleotide sequence ID" value="XM_037296046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9U5"/>
    <dbReference type="GeneID" id="59333540"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000008">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="12"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="15">
        <location>
            <begin position="93"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6219309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="133" mass="14071" checksum="6A81A77B8870B569" modified="2022-01-19" version="1">MTGGKSGGKASGSKTAQSRSSKAGLAFPVGRVHRLLRKGNYAQRVGAGAPVYLAAVLEYLAAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGHVTIAQGGVLPNIHQNLLPKKTTKGGKTASQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9X8</accession>
    <name>A0A8H6C9X8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148974.1">
        <property type="nucleotide sequence ID" value="XM_037294927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9X8"/>
    <dbReference type="GeneID" id="59332417"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="90" mass="9389" checksum="F5D21CE5A1D3448E" modified="2022-01-19" version="1">MGAVVSCAETSLLNLNHRPQIPDFSPLTCHQSQIESLFQTLGSCLMAIVNGIGSVLQAIIAGIVSCFDIIISCLTCGRGGGRRRGGRSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Z8</accession>
    <name>A0A8H6C9Z8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148841.1">
        <property type="nucleotide sequence ID" value="XM_037296143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Z8"/>
    <dbReference type="GeneID" id="59333638"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037647">
        <property type="entry name" value="HIRIP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410:SF2">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="48401" checksum="C9F906A4EB7AABEE" modified="2022-01-19" version="1">MSESSLSDGAPSEVVLEQALRNAVREVYRSGDLDQLTVKRIRKFVETDLDLQDDFFKNDPAWKGKSRNLIQSEVDSQADANANGEQPSSIPSPRAPPKSKRAQSNLPKPQKPNRDFRGTKRASSEKAGPNKRQKKETLDPEGSDSEEKEAPRKKPIPKPKKRACSEKTGPKERHQEEISDLDGPGSEEQEVLSTKPVSKLGKSTSSPSPEPETIEKADQISNHISGNSKANGKKAEASESEMSEVLDEAPKRKGRKRKSGSAKPSASTNGAKADASDSEMSEVLDEARKAKGQKRKSDSAKPSAKKTETLKRKKSSEQSTDPGAEEIKRLQGWLIKCGIRKMWHKELAPYDNSKAKIVHLKKLLADAGMTGRYSVEKANQIKDERELRADLEAVQEGAKQWGKAEAEGEDETSGRPKRRLARGLQELAFLNDDDGEETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA26</accession>
    <name>A0A8H6CA26_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_003910</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148879.1">
        <property type="nucleotide sequence ID" value="XM_037294832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA26"/>
    <dbReference type="GeneID" id="59332321"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15874">
        <property type="entry name" value="R-SNARE_Snc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="13442" checksum="49733CEB5FB7CE18" modified="2022-01-19" version="1">MAGRELPYDPYIPSGGSGQGGSAGYEGGNTRTAAIQSQIDDTVNVMKKNLESVAQRGEHIDQLQDKSENLNQSAQGFRRGANRVRKQMWWKNMKMRIWIIVGIIVLLLIIIIPSVVASKHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA32</accession>
    <name>A0A8H6CA32_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003987</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148953.1">
        <property type="nucleotide sequence ID" value="XM_037294906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA32"/>
    <dbReference type="GeneID" id="59332396"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="586" mass="66044" checksum="368207F7EA0D25A2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA98</accession>
    <name>A0A8H6CA98_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003663</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149273.1">
        <property type="nucleotide sequence ID" value="XM_037294585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA98"/>
    <dbReference type="GeneID" id="59332074"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="83"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="18050" checksum="30317A09270563B7" modified="2022-01-19" version="1">MGDQAIQPTPESPEIIAFATRMYDAARQGDMPIFEQALPAGLPPNMTNDKGDSLLMLSTYHGHAPLSRLLLSHGADPNRLNDRGQSPLAGAVFKNEQEIMDILLEAGADVDLGRPSAWEAVELFKKTDEYGEKFSRQREKLKGEKGGGGKGEAEAKGGVGGTKGAGQMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAB9</accession>
    <name>A0A8H6CAB9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003693</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149303.1">
        <property type="nucleotide sequence ID" value="XM_037294615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAB9"/>
    <dbReference type="GeneID" id="59332104"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="355" mass="37738" checksum="40F2C9F8EB762E0C" modified="2022-01-19" version="1">MPSENALSAGLADSIRPEVQYIQKNIYALNDFFTTYYNALTAAQSDASTTIQGLINEVDPIKPATFNLITLLTVLSVGLAFLGAPSIAVTVLGLSSLAKSAAQVLTISLQQAPSTAKALWPTGTQNSQSVQIGNLEAELGNSTNQLADMVNNAVQLLMSDMPTFVKFVESGQYSGAESLSLPSKVEGLDYALRTYMTSEAFAQNGWVSTPMIGPFETVQDVENAVMGGGGGDCTMGNNSVCTNGQGSALFWSQPTGRVYAFANENDAWIGVDAYQLLQDTVNNNWAILQVLFDGSFNCTQEGNFGDSIVNFNWDGTLDIACISQLPTKVECGAACPVPLINNQCPFPPLNNDCDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAK9</accession>
    <name>A0A8H6CAK9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003554</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149164.1">
        <property type="nucleotide sequence ID" value="XM_037294476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAK9"/>
    <dbReference type="GeneID" id="59331965"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099:SF7">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="57506" checksum="CD297096679DB66E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAX8</accession>
    <name>A0A8H6CAX8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149321.1">
        <property type="nucleotide sequence ID" value="XM_037294633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAX8"/>
    <dbReference type="GeneID" id="59332122"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="211" mass="23619" checksum="DA262E03141363B0" modified="2022-01-19" version="1">MTDIVADSANTVPISAIAVLGFFVGISIILIIGKVIKGRRDPKRKKAMVADQQWLSDTGKDDLSPDHRARHEAGSPTQLHLSVNHQNPQAKHHRTNVFNLTYVLTYLQASTPTSYDSSREYSGVVPKDPSSKHTMLCLMAVTALALSHICDWRERRKRDQRQQDRLAALENGMMDLTGRLLPNPGYPLLDEDDDFKLPPPAYELHEGIPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB65</accession>
    <name>A0A8H6CB65_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Oxidoreductase acuF-like C2H2 type zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149753.1">
        <property type="nucleotide sequence ID" value="XM_037294372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB65"/>
    <dbReference type="GeneID" id="59331861"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058925">
        <property type="entry name" value="zf-C2H2_AcuF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35391:SF7">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35391">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26082">
        <property type="entry name" value="zf-C2H2_AcuF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase acuF-like C2H2 type zinc-finger" evidence="2">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="861" mass="97109" checksum="1479A8968B10ED6D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB78</accession>
    <name>A0A8H6CB78_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ubiquitin-conjugating enzyme E2 2</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Ubiquitin carrier protein UBC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Ubiquitin-protein ligase UBC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_003197</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149501.1">
        <property type="nucleotide sequence ID" value="XM_037294120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB78"/>
    <dbReference type="GeneID" id="59331608"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000007">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0749">Sporulation</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="22">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="20">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAF6220066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="166" mass="18517" checksum="19148206E2E82201" modified="2022-01-19" version="1">MTTPARRRLMRDFKRMQTDPPAGVSASPIADNVMTWNAVIIGPADTPFEDGTFRLVMHFEEQYPNKPPGVKFISQMFHPNVYGTGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNTSSPANVEASNLYKDNRREYTKRGSGNSGEELGRLGRIIISLDHTQACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBJ6</accession>
    <name>A0A8H6CBJ6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149631.1">
        <property type="nucleotide sequence ID" value="XM_037294250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBJ6"/>
    <dbReference type="GeneID" id="59331738"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="294" mass="30682" checksum="E60FA043ECC8B3D1" modified="2022-01-19" version="1">MSTEASPKGKGGRAPVNAGNDADSIMPLLFATIGNPAISFKKMAAMDELGRTESSLEHKFRKWRQKGREIAAENPENAGTLGTANAGGATTKKPRAPAKKGANGKDEGPVKQAGVEDEEDDIDEEAGAIKQESDEMSGINGEMLPPPTKGKATATKGTKKRAASEKSADAEHERTEAPVKKAKVVKKGTAKVVKKSNAKLYSGSEDEEAVSEDEEKVPTTKSKGKGKSNVAAAHGKGKAAAKPTGPAKAKKGKADMAAEAGQIPIHEDEDADVDVNSEDGHHDGMIDMQFDEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC93</accession>
    <name>A0A8H6CC93_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002598</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150353.1">
        <property type="nucleotide sequence ID" value="XM_037293524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC93"/>
    <dbReference type="GeneID" id="59331011"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="19"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="363" mass="38331" checksum="FF065D35CE1DA003" modified="2022-01-19" version="1">MPSAQFSLPAKQTAVVADGAGKLSVRRDASVPILGPDVAMIRTAAVAINPVDAKMLDYSPAPGAIHGYDFAGTIVALGKDTPAHLKVEDHVAGFVHGMNPLLPDVGAFAEYVAASADLLLKVPDGLSFEEASTIGLGLFTAGLGIFRELQVPASLDPPGTLDPFISEFVLIAGGSTATGTRAIQLLKLAGLRPIATCSRSNFGLCRRFGAEEVFDYSSPECSADIRAYTRNTLAYALDCVAAADTTQLCYSAIGRAGGRYVTVEPFHEAITSARPLTVEPSWLLALTVFGRKVNLDGEYGRDANPEDRKFGAKLTADVQALLDQGKIDTHPIQVMTGGWNGMIDGVNIIREQAPSGRKLVYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCZ7</accession>
    <name>A0A8H6CCZ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Meiotically up-regulated gene 190 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_002180</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150760.1">
        <property type="nucleotide sequence ID" value="XM_037293107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCZ7"/>
    <dbReference type="GeneID" id="59330594"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04041">
        <property type="entry name" value="C2A_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21676">
        <property type="entry name" value="SMP_Mug190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057349">
        <property type="entry name" value="C2_Mug190_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037767">
        <property type="entry name" value="C2A_Mug190-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47348">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 190 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47348:SF3">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 190 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25331">
        <property type="entry name" value="C2_Mug190_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="14">
        <location>
            <begin position="246"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="472"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="661"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="101"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="631"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="697"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1024"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="647"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="703"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1024"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1071"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1101"/>
            <end position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6221325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1207" mass="134119" checksum="F879DB39AE82E395" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD20</accession>
    <name>A0A8H6CD20_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED16</name>
        <name evidence="12" type="ORF">HO133_002685</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD20"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048338">
        <property type="entry name" value="Mediator_Med16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048339">
        <property type="entry name" value="Mediator_Med16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021665">
        <property type="entry name" value="Mediator_Med16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13224:SF6">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13224">
        <property type="entry name" value="THYROID HORMONE RECEPTOR-ASSOCIATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20719">
        <property type="entry name" value="Med16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11635">
        <property type="entry name" value="Med16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med16 N-terminal" evidence="10">
        <location>
            <begin position="57"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit 16 C-terminal" evidence="11">
        <location>
            <begin position="744"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6221004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="844" mass="94328" checksum="5D6576FF27FDD524" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDH7</accession>
    <name>A0A8H6CDH7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Extragenic suppressor of kinetochore protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002285</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150864.1">
        <property type="nucleotide sequence ID" value="XM_037293211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDH7"/>
    <dbReference type="GeneID" id="59330698"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="450"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="936"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="555"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="578"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="601"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="944"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="955"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="978"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="988"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1022"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1045"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1106"/>
            <end position="1129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1129" mass="124098" checksum="4578E5A4BFED1ECB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDW2</accession>
    <name>A0A8H6CDW2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001586</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151055.1">
        <property type="nucleotide sequence ID" value="XM_037292516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDW2"/>
    <dbReference type="GeneID" id="59330002"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="533" mass="57523" checksum="AC913B3DF19B31CA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDZ5</accession>
    <name>A0A8H6CDZ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-protein ligase E3A N-terminal zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001803</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151270.1">
        <property type="nucleotide sequence ID" value="XM_037292731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDZ5"/>
    <dbReference type="GeneID" id="59330217"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.130.10">
        <property type="entry name" value="Ubiquitin-protein ligase E3A, N-terminal zinc-binding domain (AZUL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032353">
        <property type="entry name" value="AZUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042556">
        <property type="entry name" value="AZUL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056012">
        <property type="entry name" value="DUF7590"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF15">
        <property type="entry name" value="FUSION DEGRADATION PROTEIN (UFD1), PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16558">
        <property type="entry name" value="AZUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24503">
        <property type="entry name" value="DUF7590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23580">
        <property type="entry name" value="Znf_XAF1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="4">
        <location>
            <begin position="171"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7590" evidence="3">
        <location>
            <begin position="272"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-protein ligase E3A N-terminal zinc-binding" evidence="2">
        <location>
            <begin position="652"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="788" mass="87597" checksum="838F719766B1E162" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEM2</accession>
    <name>A0A8H6CEM2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rhomboid-type serine protease</fullName>
            <ecNumber evidence="10">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_001158</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Serine protease involved in intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151507.1">
        <property type="nucleotide sequence ID" value="XM_037292088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEM2"/>
    <dbReference type="GeneID" id="59329574"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936:SF69">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="342"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="12">
        <location>
            <begin position="244"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6222072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="510" mass="56954" checksum="97C94A12ABA7BEBB" modified="2022-01-19" version="1">MAANEYYNPSPSPGNGQRYNAPPPRLPLDPYATYASHPTQPSPPQISSPYEDTSYRPYADQSQQSIPSPYYASGGGGRETEPNPYSDDIPLRQHPSKGSSEMLHDPLPHDPAIIDRPTQNAARRRRKQGFFGGGKIPWVVYTLTLIQVCVFIAELVRNGVLTKTPIEIHPSFNPMIGPSPYVLINMGARYVPCMRQTSEGLDTTIDWPCPNTTSTSLDDSSNQCQLNNLCGFHDKAYTKSNPPNQWFRFIIPMFLHAGFIHIAFNMLLQILLGKEIEILIGSIRFLIIYLSSGIFGFILGGNFAPQGIASTGASGSLFGIIAIMLLDLLYHWEERQSPWIDLAWLMFDIVISFALGLLPGLDNFSHIGGFLMGLVLGICLIHSPEILRQRVGADLPPYASVQQARKLADNGDIKAFVKEPVAFFKGRKPLWWAWWLVRAAALIGAFVVFIVLLKNFYKADPKTCHWCRYLSCIDIKNWCDIGNLQTTNGTTGSKRDLLSRPLVTKLEYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG71</accession>
    <name>A0A8H6CG71_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NIPSNAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001026</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NipSnap family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152321.1">
        <property type="nucleotide sequence ID" value="XM_037291959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG71"/>
    <dbReference type="GeneID" id="59329444"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000004">
        <property type="entry name" value="NIPSNAP family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000037">
        <property type="entry name" value="NIPSNAP family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051557">
        <property type="entry name" value="NipSnap_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017">
        <property type="entry name" value="NIPSNAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017:SF17">
        <property type="entry name" value="PROTEIN NIPSNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIPSNAP" evidence="3">
        <location>
            <begin position="123"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="NIPSNAP" evidence="3">
        <location>
            <begin position="228"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="327" mass="37771" checksum="8409D605A7DA35C0" modified="2022-01-19" version="1">MLTRRLFTSSSTPSTLLRTFTTSTAHQRSPALGDITPNGAAKFDARQTAFREEVREAQKAQEKQDRASRETSQQQESSKHKGALSSLIYGTHEGQQMDHEIEKSFSQVLARGKYVHSIVMHSVKPDKVDEYVDLVGDWYPKVASTPEHHVHLVGSWRTEVGDCDTFVHIWEYQRYQGYHSSLHSIQHHPDFPAFDHKLKSLITHKETSLMQEFSFWPTTPPRQLGGLFELRSYTLHPGNLLEWETHWRRGLKARREVMEGVGAWFVQIGDLNTVHHLWQFADLEERKARREMSWGVEGWGDTVHKTVPLIQKMKSRILIPMPWSPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH84</accession>
    <name>A0A8H6CH84_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="19">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HO133_000264</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="19"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152639.1">
        <property type="nucleotide sequence ID" value="XM_037291204.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59328683"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000067">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 18 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1154"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1203"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1231"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1257"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1287"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="177"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="21">
        <location>
            <begin position="318"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="23">
        <location>
            <begin position="1091"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="276"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="404"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="16">
        <location>
            <position position="649"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="649"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="649"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="650"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="651"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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            <name>Mg(2+)</name>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAF6223422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1327" mass="146180" checksum="68E5B8C6B180C47F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHI5</accession>
    <name>A0A8H6CHI5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 16">Elongator complex protein 3</fullName>
            <ecNumber evidence="16">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_000447</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="16 17">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ELP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="16"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152821.1">
        <property type="nucleotide sequence ID" value="XM_037291386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHI5"/>
    <dbReference type="GeneID" id="59328866"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000003">
        <property type="entry name" value="Elongator complex protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="14 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 16" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 16" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="19">
        <location>
            <begin position="103"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="18">
        <location>
            <begin position="417"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6223604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="583" mass="65774" checksum="9EAAF84749C21D70" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHM6</accession>
    <name>A0A8H6CHM6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000510</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152884.1">
        <property type="nucleotide sequence ID" value="XM_037291449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHM6"/>
    <dbReference type="GeneID" id="59328929"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000015">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="7">
        <location>
            <begin position="83"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="5">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="324" mass="36292" checksum="59AAA30530D7CA2D" modified="2022-01-19" version="1">MNHSPQKHIHPYHRPLPAQSQTTDKGRAEAEAYYNSDSQSNMSPSKLALTDLLPLHNSSVKIPRLGFGIYQSPTNVCINSCLTALKSGYRHIDSAQFYRNETEMGEAARQSGIPRNEVFLTTKIMSAGGSAEKTYKKCLDSVHKIDGEEGYVDLFLIHTPSGGSAAVKEMYQALERLESEGKAKSIGVSNFGIRHIEEMKEYARIWPPHVNQIELHPWNQQRTIVDYCNQQKVVIEAYCPLVRNLKAHDQTLVEMSKKHDKTTAQILIRYSLQKGWVSLPKSDTPSRIEANADVYGFSLTDEDMETLDSLDQGAKGAIVQAVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHV0</accession>
    <name>A0A8H6CHV0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-conjugating enzyme E2 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ubiquitin carrier protein UBC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Ubiquitin-protein ligase UBC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_000369</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152743.1">
        <property type="nucleotide sequence ID" value="XM_037291308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHV0"/>
    <dbReference type="GeneID" id="59328788"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23811">
        <property type="entry name" value="UBCc_ScCDC34-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000063">
        <property type="entry name" value="CDC34p Ubiquitin-conjugating enzyme (E2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="2"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="160"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="189"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6223526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="236" mass="26393" checksum="143DF011178D43AC" modified="2022-01-19" version="1">MAASKILQNEYKALSQEKWVNVELNNDDIFNWNVALIVINPDSLYYGGYFKAKMNFPHNYPYTPPSFQFNRALFHPNIYADGKLCISILHAPGDDEMSGESAGERWSPAQRVESILLSIISLLDDAECSSPANVDAGVMLRKNTEGFKKKAKADVEKGKEDIPAGFVMPTHESTARIPEKPVDDREFWVDSDDEDDFGGSDTDAEMTEDGNDGLPDDEDQEEGDDEDPENEDDETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ35</accession>
    <name>A0A8H6CJ35_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_010715</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153201.1">
        <property type="nucleotide sequence ID" value="XM_037301567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ35"/>
    <dbReference type="GeneID" id="59339105"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904679">
        <property type="term" value="P:myo-inositol import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000073">
        <property type="entry name" value="MFS myo-inositol transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="43"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6224141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="533" mass="57410" checksum="006A5B6B377051D7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ51</accession>
    <name>A0A8H6CJ51_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_011039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153522.1">
        <property type="nucleotide sequence ID" value="XM_037301888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ51"/>
    <dbReference type="GeneID" id="59339429"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="214" mass="23819" checksum="A8046FB2F29233A0" modified="2022-01-19" version="1">MDLYSVRGVTHRIDQELSAEEPPGAKYQTARSLRIHHNSYALHVRFGTEDKMAPRLDYQRGSHEARAEPVPLQIRALSIASKPPGWRLAREAHRVHITATCNDRLSHTASRLYPYRRVAHPPFNTIGAACFWIEIPPGSTPPQTEPLPVFPPLPLPSTCNTPARFLLASLPNNPNPLNQILASVTGARSVRCQKAARWVPLVGWVGIKLPISDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ80</accession>
    <name>A0A8H6CJ80_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010776</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153261.1">
        <property type="nucleotide sequence ID" value="XM_037301627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ80"/>
    <dbReference type="GeneID" id="59339166"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="6">
        <location>
            <begin position="327"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="7">
        <location>
            <begin position="396"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="233"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="446"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="511"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="465"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="532"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="573"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="598"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="608" mass="66946" checksum="DD8B38573EAC6753" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKF4</accession>
    <name>A0A8H6CKF4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Saccharopine dehydrogenase NADP binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010160</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153832.1">
        <property type="nucleotide sequence ID" value="XM_037301015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKF4"/>
    <dbReference type="GeneID" id="59338552"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="4">
        <location>
            <begin position="12"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="518"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="543"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="697" mass="75682" checksum="3E2B44C3E6881048" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKK1</accession>
    <name>A0A8H6CKK1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154025.1">
        <property type="nucleotide sequence ID" value="XM_037301208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKK1"/>
    <dbReference type="GeneID" id="59338746"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4100.10">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042183">
        <property type="entry name" value="MmgE/PrpD_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045336">
        <property type="entry name" value="MmgE_PrpD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943:SF8">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03972">
        <property type="entry name" value="MmgE_PrpD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MmgE/PrpD N-terminal" evidence="2">
        <location>
            <begin position="16"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="MmgE/PrpD C-terminal" evidence="3">
        <location>
            <begin position="292"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="492" mass="54016" checksum="79696D890348E9E9" modified="2022-01-19" version="1">MGAISGERPEAPGTTEHLCRWIHHLQFDDVPEDIRTRAKHLILDGLACAIVGAHMPWSEHAASSMQAFEPNGNATIFGHPYKLGPLAAALLNGAFIQACELDDYHHEAPLHSASVILPTLFAASESQSHARPPAPVTGSTFLLASIVGFEVGPRAGRALYGTELLSMGWHCGTVFGHPASAAAASKLFALSARQTEDAVGIACTQACGLMSAQYEGMIKRMQHGFAARNGLLGAFLAKGGYEGIRKVFERPYGGFLTMFSKGNSRTPAYVVEEVTKGLGTTWDTAEVRIKTHACVGGAYGLVECIEKMQKEKPEELKDLQAIKEIRLELSKPLIGHCGWTATRPMTATGAQMNANYIAAVQLVDQEVLLEQFEESKLDRDEVWELVEKTVCVHNERFDRLSYEMGAVVTIDFKDDRLSLQKVVEKPRGIDPPIANEEIVEKYRRLTANLLPRDRQIAIEKTVLNLEKLDNVITLTSLLSQPFKLDHPVRPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL29</accession>
    <name>A0A8H6CL29_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154233.1">
        <property type="nucleotide sequence ID" value="XM_037300385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL29"/>
    <dbReference type="GeneID" id="59337919"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="142" mass="16375" checksum="DCAD99A386D6AD5D" modified="2022-01-19" version="1">MDSRPQNCYPVVVTESSRFYRDPDDEETFVPLHKTTHIEEVTFSRNFANALAKEYFIRQHCESVSDSNEDDFGYPTPRARRWGIWVPENTTKCTISEVEKRCRIKVVLGSGSERTEFLVEVLRQNICDAVGPLAREDSDPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLB9</accession>
    <name>A0A8H6CLB9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009593</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154302.1">
        <property type="nucleotide sequence ID" value="XM_037300454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLB9"/>
    <dbReference type="GeneID" id="59337988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021838">
        <property type="entry name" value="DUF3431"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11913">
        <property type="entry name" value="DUF3431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="297" mass="33614" checksum="EE048E4B0D3FA36A" modified="2022-01-19" version="1">MRLALRFALSASVFLIILLLIVKIKLEDVWDQYGVSNYIAHSWKNTFQHGPVQNIPTFDGEASHKVVIMAKMEKENTDWVAENLPDWQAAIYTVNPSPNTTDTTLTTPMNKGRESMAYLSYVIDNYANLPSTLVFLHSHRSGFFSAWHTDTPLHDNVDALRSLQIPFVQKNGYVNLRCNWNPGCLEAHRHNAHVTPEVWKDVFAGTSKEEKAPSNTNGQAPTLVGAACCAQFAVSKDQVLARPLSDYERFREWIIDTEKSDAKSGRVLEFLWHVIFGKDAVYCPAEEKCYCDVYGHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLQ7</accession>
    <name>A0A8H6CLQ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ring-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154439.1">
        <property type="nucleotide sequence ID" value="XM_037300591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLQ7"/>
    <dbReference type="GeneID" id="59338126"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="49308" checksum="58272244176574E3" modified="2022-01-19" version="1">MMEYFTSKRARSKSPAPQTQVETPVLNEEDEAFLARIETEGTPPELPARPAPPLPTRPQDLPHVGETEGNNAQIALLDGSQNIALPDVPDTPDDVLTTAEPDDIDEGNAKGKQKEGKKSKWSFLRRDSRDVKRKNKEATATDLMSAAEGLKSADAQPNEDHTVSDTEEKKEEEEMSSVLEQLNLAAVNNRVFSVSKESQELLRKFTLVLKDLTNGVPTAYGDLESLLTNSEDQIQRTYTHLPPWLQKLVEQLPSKMTKTIGPEMLAAAAEKHGINSKYANMAASGASRAGLKVRVPSLKDLVTKPGAVAAMLKAIMNFLKLRFPAFLGMNVLYSLALFVLLFVFWYCHKRGKEVRLEKERLLTEAEIAALDDDAPTTSSNAPPTTTAPTGAPIEQVEAGMREAEAVEQQQGGRGQFAEDGSLAQELRREEREKAMLSHAARTRGTDHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLZ5</accession>
    <name>A0A8H6CLZ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phenylacetyl-CoA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009686</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154395.1">
        <property type="nucleotide sequence ID" value="XM_037300547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLZ5"/>
    <dbReference type="GeneID" id="59338081"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05911">
        <property type="entry name" value="Firefly_Luc_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.38.10">
        <property type="entry name" value="Luciferase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF422">
        <property type="entry name" value="BCDNA.GH02901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="55"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="486"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="64846" checksum="CE8069D38B06A25C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN44</accession>
    <name>A0A8H6CN44_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_009318</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155005.1">
        <property type="nucleotide sequence ID" value="XM_037300179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN44"/>
    <dbReference type="GeneID" id="59337713"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000692">
        <property type="entry name" value="Serine/threonine protein kinase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="17"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6226452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="550" mass="61803" checksum="F197C33B15A3593F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPC0</accession>
    <name>A0A8H6CPC0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mesaconyl-C4 CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155500.1">
        <property type="nucleotide sequence ID" value="XM_037299499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPC0"/>
    <dbReference type="GeneID" id="59337029"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000103">
        <property type="entry name" value="WGS project CABT00000000 data, contig 2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052741">
        <property type="entry name" value="Mitochondrial_HTD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152:SF2">
        <property type="entry name" value="N-TERMINAL OF MAOC-LIKE DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="37101" checksum="975952D51D3B0AAA" modified="2022-01-19" version="1">MLSRPFRRLASTAVSPRHKQLVEDFFYTASSVPSIVRKQFLDPNQIQRLSLTLNRPRLYRVGQILENEIPLNGTPLAPGYHLVYFTPAALPDHLGRDGTDISYSPAEPFTRRMWAGGRIDWVKENPLRIGDEAIETTKLVFAKPKVTRAGEEIVVVGVKKTFENAKGLALFDRRDWVFRTQLSGLPPSHDYSESTGSDDAPLPLPDTSQKSRDYLQTAVSLFRFSALTFNAHKIHYSRPWCRDVEGHRDIVVHGPLNLINMLDFWRDEQADDFMIPQSISYRATAPFYAGEKYRALLERETDTTSIKLWGHNGKGDVKVGMLGDVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPC3</accession>
    <name>A0A8H6CPC3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155416.1">
        <property type="nucleotide sequence ID" value="XM_037299415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPC3"/>
    <dbReference type="GeneID" id="59336945"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034410227" evidence="1">
        <location>
            <begin position="18"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="343" mass="36267" checksum="E49B0508A2BBF1AF" modified="2022-01-19" version="1">MFFCLILWALYSDTVLAASLSRRSESVGSLAAGPIPSSDLLSPANILVPSSNNSSSDNRLKIACDATRYGKNLKVSSCRNVFNFMKKDETTYTFAERDSGVPNDGPLPLRTLSNDGLCFVQPVLSAGAISGKASPTEIGQAALVTLQTCVIERGMGGMAFNIGGDNKVNVAIADYKPPNVKCDTTSTPGPAWNSCVAIVVNMRATKQARVFGYSGDPSVEEELPLVLEGSSIDIEGPATVMTWYELWEAVNAITSRRGNIRLELSGKKYDPGEKVGNASRVSLADAGVALQYFDTVSSQAGSTGFNRAIPFELLNLTGVMPDNGSSVTLPGDPWTNGSVMASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPJ3</accession>
    <name>A0A8H6CPJ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine dehydratase</fullName>
            <ecNumber evidence="12">4.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Threonine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_008317</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="12"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155185.1">
        <property type="nucleotide sequence ID" value="XM_037299184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPJ3"/>
    <dbReference type="GeneID" id="59336714"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00054"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04906">
        <property type="entry name" value="ACT_ThrD-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04907">
        <property type="entry name" value="ACT_ThrD-I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1020.10:FF:000001">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000008">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1020.10">
        <property type="entry name" value="Biosynthetic Threonine Deaminase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001721">
        <property type="entry name" value="TD_ACT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038110">
        <property type="entry name" value="TD_ACT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005787">
        <property type="entry name" value="Thr_deHydtase_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01124">
        <property type="entry name" value="ilvA_2Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006674">
        <property type="entry name" value="PRK09224.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF11">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00585">
        <property type="entry name" value="Thr_dehydrat_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51672">
        <property type="entry name" value="ACT_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT-like" evidence="13">
        <location>
            <begin position="374"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="ACT-like" evidence="13">
        <location>
            <begin position="470"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6226876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="551" mass="60492" checksum="4DF9A4360C9CD6C1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ25</accession>
    <name>A0A8H6CQ25_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008688</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155554.1">
        <property type="nucleotide sequence ID" value="XM_037299553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ25"/>
    <dbReference type="GeneID" id="59337083"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000008">
        <property type="entry name" value="Proteasome subunit beta type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6227246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="182" mass="20578" checksum="C80E51A8967FF3E5" modified="2022-01-19" version="1">MRGATILKAADDKTKQLNKHTLMAFSGEAGDTVQFAEYIQANIQLYSMRNDTDLGPSAVASFVRGELARSLRSRKPYTVNLLLGGMDPITNEPHLYWLDYLASLAPLPYAAHGYAQYYCLSILDKHHHPDIDFEQGLKILKMCTDELKRRLPIDFKGMLVKVIDKDGIREIDYDMSKEVLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ36</accession>
    <name>A0A8H6CQ36_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155776.1">
        <property type="nucleotide sequence ID" value="XM_037299775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ36"/>
    <dbReference type="GeneID" id="59337307"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="214" mass="24406" checksum="5EB68C2A8777834A" modified="2022-01-19" version="1">MAEMEGVEQVMREEEEEEEEEHEEEEEEQEGDIVSGPCYQVNSAVTLQQSDLCELYTCLHWRWKSEHTCWSYLEIKQMIGHASLKLDKNEYQHLDLNDVIMCLLDEEDDLTKAVKVVAEHSKDNFVKWIPSLYKRGVEAYAEETKLAKQQEDELTARQAAMGTPAAKKRRIGEFDSIATVLEGESSGSHRVKAEASRTRTGSVEDDEEGMVTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR41</accession>
    <name>A0A8H6CR41_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007843</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156049.1">
        <property type="nucleotide sequence ID" value="XM_037298713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR41"/>
    <dbReference type="GeneID" id="59336240"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000153">
        <property type="entry name" value="Salicylate hydroxylase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051104">
        <property type="entry name" value="FAD_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720:SF3">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01460)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="17"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6228115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="429" mass="46828" checksum="E5741C7BBE27825F" modified="2022-01-19" version="1">MTHPAPESAPGQKPFTIAICGGGIAGLALAIGLLRRKVPFHVYESAHAFSEIGAGVAFGPNSQRAMSLIDPKIKEGYGKRATSNGYEEKKAVWFDFHAGMDCGIGKAGEKVGTVIAADPGNGCIHRAHFLDELVALVPRENATFGKRVVEVEELEDGVRLHFADGETAEASAAVGCDGVKSNLRHAVLGKEDGAAHPDFTGKYAYRGLIPMEKAVSLLGDELARNSVMWLGNHGHVLTFPIEKGEIMNVVAFHAKKDGKWEDEKWVLPMDRQTMEADFQDWGESVKKILSLMEKPDLWALFDYPPAKTYFKGRTCLIGDAAHASTPHQGAGAGMAMEDAYVLSNLLGAVQRSSDIETAFRAYDAVRRPRTQKLVTTSRDAGELYELEKEGVWDDADKLRENLGTRYKWVWEKKLEEDLAEAMGILGGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRB0</accession>
    <name>A0A8H6CRB0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156169.1">
        <property type="nucleotide sequence ID" value="XM_037298833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRB0"/>
    <dbReference type="GeneID" id="59336362"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="86" mass="8578" checksum="62E5C6416A6038AF" modified="2022-01-19" version="1">MPHSKTPGGSGASEKFLKQQAEKHGEGASNAGGHTEGKAPGEAPVASDSGISDVGIEKGTNQPGEKGILGPKMDPVPDSSKDTRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRM4</accession>
    <name>A0A8H6CRM4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Protoporphyrinogen oxidase</fullName>
            <ecNumber evidence="4 11">1.3.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_008042</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2</text>
            <dbReference type="Rhea" id="RHEA:25576"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="EC" id="1.3.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.4" evidence="4 11"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156246.1">
        <property type="nucleotide sequence ID" value="XM_037298910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRM4"/>
    <dbReference type="GeneID" id="59336439"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004572">
        <property type="entry name" value="Protoporphyrinogen_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00562">
        <property type="entry name" value="proto_IX_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF3">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="12">
        <location>
            <begin position="8"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6228312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="465" mass="50541" checksum="5B19DA0A2B44B598" modified="2022-01-19" version="1">MPNGLITLDLVRNLGLEDRVLMTSKNSVAAQNRFIYYPDHLVRMPGPGSSLLQTILSVLSEPIFKGTISGALSEVAKPRRPEDLQDESIGSFFTRRFGSALADNVVSGVFHGIYAGDIYKLSARAILPSLWAIEWRNNSIMKGLLEEAIGGMRPIATSDLDLIKTLQSQPITSDKLEVAKKSSVYTFKGGIGELADKLEAKLDESDNVLILENTDVGQIRIVSTAAGEKIQINAKEASNRETTDYDFDHVVSTIPGNKLASIASRSPITSLSPLSRTSAVTVMVVNLFFSNPKILPVHGFGYLLPRSVPFEQNPERALGVVFDSDASIGQDDVPGTKVTVMLGGHWWDDFDAYPDEDEGASMAKALLKRHLGVNEEPRAIRVSLQRSCIPQYGVGHEARMEDASLRLDSFKGRLRVAGNSYTGVGLNDCIRAARDVVKGLVDGTGTTGLESFVGGKKWSWISETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRM8</accession>
    <name>A0A8H6CRM8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial intermediate peptidase</fullName>
            <ecNumber evidence="4">3.4.24.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Octapeptidyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_008053</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.</text>
            <dbReference type="EC" id="3.4.24.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase M3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.59" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156257.1">
        <property type="nucleotide sequence ID" value="XM_037298921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRM8"/>
    <dbReference type="GeneID" id="59336450"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06457">
        <property type="entry name" value="M3A_MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033851">
        <property type="entry name" value="M3A_MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF79">
        <property type="entry name" value="MITOCHONDRIAL INTERMEDIATE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 15" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="16">
        <location>
            <begin position="293"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6228323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="778" mass="87009" checksum="168147C80644C657" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS02</accession>
    <name>A0A8H6CS02_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156411.1">
        <property type="nucleotide sequence ID" value="XM_037299075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS02"/>
    <dbReference type="GeneID" id="59336604"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395:SF1">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="457"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="601" mass="65863" checksum="51E92AE1700F7414" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSP4</accession>
    <name>A0A8H6CSP4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006996</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RENT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156526.1">
        <property type="nucleotide sequence ID" value="XM_037297891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSP4"/>
    <dbReference type="GeneID" id="59335396"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12455">
        <property type="entry name" value="RRM_like_Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039722">
        <property type="entry name" value="Upf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112:SF0">
        <property type="entry name" value="FI21285P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112">
        <property type="entry name" value="UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="445"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="199"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="328"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="523"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="272"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="371"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="528"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="570"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="615"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="626" mass="64137" checksum="48ECCF8AA51E05F2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CTD2</accession>
    <name>A0A8H6CTD2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Altered inheritance of mitochondria protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156551.1">
        <property type="nucleotide sequence ID" value="XM_037297916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CTD2"/>
    <dbReference type="GeneID" id="59335421"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF5">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034905477" description="Altered inheritance of mitochondria protein 6" evidence="2">
        <location>
            <begin position="19"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="34541" checksum="6164A415037E593B" modified="2022-01-19" version="1">MRTSTIFSALAAAPYALAVAPDVSTALQQILNQAHQGPLYTYPTSLTQGIVPKLIHSHNDYWRDVPFYSAISVGAVSVEADVWLYNGTLHIGHEEGALTNARTFESLYINPILDTLQRQNPTNSSFLTAPTHNGVFDTNGAQTLYLFVDVKTPGNTTWPYVVKALEPLQSGGWLSTVNSSNQFTLGAVTVIGTGNTPLNQVQPVTPRYYFYDAPIPTLNSTFSNITSLVSPIASTDFEANFGPVPGTSLNDTQLALLRAQVATAHAKGIMLRYWDQPGWPISTRNGIWRQLKSEGVDLINVDDLEGGAGFSDESNYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7I5</accession>
    <name>A0A8H6F7I5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006063</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147540.1">
        <property type="nucleotide sequence ID" value="XM_037296966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7I5"/>
    <dbReference type="GeneID" id="59334468"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF1">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="58540" checksum="FBC580555352BA31" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7Q1</accession>
    <name>A0A8H6F7Q1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF4045 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006473</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="entry name" value="Villin/Gelsolin"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4045" evidence="2">
        <location>
            <begin position="10"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7904" evidence="3">
        <location>
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            <end position="1363"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="728"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="1182"/>
        </location>
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            <begin position="23"/>
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            <end position="93"/>
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        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
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        <location>
            <begin position="162"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
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        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
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        <location>
            <begin position="312"/>
            <end position="347"/>
        </location>
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        <location>
            <begin position="421"/>
            <end position="447"/>
        </location>
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            <begin position="502"/>
            <end position="513"/>
        </location>
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            <end position="628"/>
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        <location>
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            <end position="671"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="675"/>
            <end position="687"/>
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        <location>
            <begin position="688"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="768"/>
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        <location>
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        <location>
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    </feature>
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        <location>
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        <location>
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        <location>
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        <location>
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            <begin position="1166"/>
            <end position="1178"/>
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        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF13254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1653" mass="177265" checksum="3B685EB601190B37" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7X3</accession>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">HO133_006366</name>
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        <name type="scientific">Letharia lupina</name>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147389.1">
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11060">
        <property type="entry name" value="CYP57A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="p450"/>
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    </dbReference>
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        <property type="entry name" value="EP450I"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
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    </dbReference>
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        <property type="entry name" value="CYTOCHROME_P450"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="540"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
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        </ligandPart>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6217954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="599" mass="68125" checksum="A745D852128845BF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7X8</accession>
    <name>A0A8H6F7X8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor RSE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pre-mRNA-splicing factor rse1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_006455</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in pre-mRNA splicing and cell cycle control.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the RSE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037297353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7X8"/>
    <dbReference type="GeneID" id="59334856"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001143">
        <property type="entry name" value="Pre-mRNA-splicing factor rse-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000068">
        <property type="entry name" value="Pre-mRNA-splicing factor rse1, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="12">
        <location>
            <begin position="19"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="13">
        <location>
            <begin position="462"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="11">
        <location>
            <begin position="854"/>
            <end position="1175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6218043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1209" mass="133927" checksum="447F5DE59A115B11" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F851</accession>
    <name>A0A8H6F851_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147629.1">
        <property type="nucleotide sequence ID" value="XM_037297055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F851"/>
    <dbReference type="GeneID" id="59334557"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="330" mass="35893" checksum="DC6790275E7BE385" modified="2022-01-19" version="1">MHWSKTPIPGVSLDATGLIALADLKTIAKHTALNGTSSIVDFLVLCPGIHTQPNATELNGGELPPTAALTSGHVFRVENQATVFYLQKMGIPGQLVTMSVEGLPEPKRWKPDLKTIKSTLTPSFVCYITPVSLTLLALMFVSFLNDWWALAVLLILILARTLNAIVVRRRSAPGWKGVKEPGVKGDLLILLSQDRWVRLQGPVDALKAVTAGQWLRDATFLESSLTAIALLLIYINAAVTTNASQAGNMIILALLLLSVGLVAMSNQWQDSLYMHGCKVQVDGEPKQYARRLDLADELISSTGRDDWAIRLGLINEKAEAATNTVKRVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F884</accession>
    <name>A0A8H6F884_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_005967</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148051.1">
        <property type="nucleotide sequence ID" value="XM_037296870.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59334372"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000481">
        <property type="entry name" value="Structural maintenance of chromosomes 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000585">
        <property type="entry name" value="Structural maintenance of chromosomes 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF172">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="14">
        <location>
            <begin position="737"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="696"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1084"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="398"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1193"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="103"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="543"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6218616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1411" mass="157909" checksum="586A337E6BCE6088" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8M5</accession>
    <name>A0A8H6F8M5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148243.1">
        <property type="nucleotide sequence ID" value="XM_037296261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8M5"/>
    <dbReference type="GeneID" id="59333757"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="245" mass="27836" checksum="C9BD842F60FF5C3B" modified="2022-01-19" version="1">MFYKFHSKSPWLKRVLVPFWTFQLIFMVALISFFVWVLAYVGFHRNTNIVVLIISGVCMVLSVIEIILFVVIRLDPLTYLILQLVKTIMWLVLFALAAAESRRVQKATKVDEDQGYSSGITPEYLVLAGLGETLVLFVTFLAALTYASIVYHRHRRAKARFLRSVQGADLYPVIADEDASPVGSHRIFRALRNSIKRLSPEAEVPYPAELKEAVEVTVLRELGGDWDVYEPPGTRSVRSFKGQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8P8</accession>
    <name>A0A8H6F8P8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DHHA2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005411</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148303.1">
        <property type="nucleotide sequence ID" value="XM_037296321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8P8"/>
    <dbReference type="GeneID" id="59333817"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.20">
        <property type="entry name" value="DHHA2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004097">
        <property type="entry name" value="DHHA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038222">
        <property type="entry name" value="DHHA2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF39">
        <property type="entry name" value="EG:152A3.5 PROTEIN (FBGN0003116_PN PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01131">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA2" evidence="5">
        <location>
            <begin position="253"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="415" mass="45968" checksum="CB2D321F18E0D9B4" modified="2022-01-19" version="1">MTQHAKTNLVKAVHEEHNINIVIGNESADLDSLTSSLLYAYIKSIAPPRNNFSPIYVPLLNIPASGLRLRPEFSAVCAHANIAIDRLITLDDLPSQSRLLKSVRWILVDHNKLQGDLGDQFSSKVLGVIDHHEEENAVPQNTGSEPRVIEKCGSCTSLVVRECKSAWDSISSSSLSTGAAHGQGEVAVDDSAVTQVWDAQVAKMALASILVDTANLTAPGKVEHVDRDSVDYLEAKIQLSPKDAQSWNRDRYYKDIDEAKRDISSLSLTEVLIKDYKGWTESGTKLGISSVVKPLEFLVQKAGEENKSSFEDQLQHFMDERDLSMFAIMTTSTSSEGEFQRELLLQARSSSSSSAIKFAEQGASDFQLEILNIKGVLIQDKPGQHWKKVWLQKDVSKSRKQVAPLLRKVMTRPNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9Y8</accession>
    <name>A0A8H6F9Y8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rax2-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002848</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149851.1">
        <property type="nucleotide sequence ID" value="XM_037293773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9Y8"/>
    <dbReference type="GeneID" id="59331260"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902929">
        <property type="term" value="C:plasma membrane of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024982">
        <property type="entry name" value="Rax2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778:SF2">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12768">
        <property type="entry name" value="Rax2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034609651" description="Rax2-like C-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="844"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="domain" description="Rax2-like C-terminal" evidence="3">
        <location>
            <begin position="575"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="887" mass="92975" checksum="6409B4E01367481D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9Z0</accession>
    <name>A0A8H6F9Z0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sphingoid long-chain base transporter RSB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003180</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150182.1">
        <property type="nucleotide sequence ID" value="XM_037294104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9Z0"/>
    <dbReference type="GeneID" id="59331592"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF9">
        <property type="entry name" value="SPHINGOID LONG-CHAIN BASE TRANSPORTER RSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="33954" checksum="3075D38BAAAAA833" modified="2022-01-19" version="1">MLSLSLLLPRGQDCTNISADCPVSQSFYGYSPSLGPDITLLVLFSMALVGHGVQGWYYRAWGTLTAMLWGCICEVLGYIGRLMMHPNAFNLNGFLIQICCLTIAPAFFSAAIYLCLSRLVLLFGTEVSRLPPKYYAYIFISCDIVSLALQGAGGGLASVAAQNDTSLTVGDNFQLAGLAFQVASLTLFAVLSAEYAFRVYRSPQASARAPGIFRHNKGLMYFLVALATSWLFIEIRCIYRVIELAGGWGSSLMKKQTDFVVLEGAMIIIAVGVLNAFYPGKYLNRAVDPEKLDEKKASDTELATPTMSATVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAB2</accession>
    <name>A0A8H6FAB2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149968.1">
        <property type="nucleotide sequence ID" value="XM_037293890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAB2"/>
    <dbReference type="GeneID" id="59331378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034362523" evidence="2">
        <location>
            <begin position="22"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="298" mass="33016" checksum="4F8997F4A0731B13" modified="2022-01-19" version="1">MLFKPLSLLLFALALLHSVGAEDTVSSGNYLIHLCNSGQPDSEASKLQDLLPQVYNGLQKVIADLQLGTASTHGYSAFFKDDSSKAKILRVYQRMAVGASVALGGQGRNINPITIRHPTFVCANNVPETDLLYQHCMHAPNSPLMSWMQTELMPLCPLFWTIKKQARLSDCPLVVANTLTPNDDRLLKNQEALLVGNLVHLYHRVSGDMVVTITDASALNVSESLKHPPNYALYYACEHLTPMDASASDKRWAGSKLGERELSAKYGRNRRGGRREWSDQHKEKEDDQLSRGTIVSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAI0</accession>
    <name>A0A8H6FAI0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein/NADH dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002187</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150767.1">
        <property type="nucleotide sequence ID" value="XM_037293114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAI0"/>
    <dbReference type="GeneID" id="59330601"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040049">
        <property type="entry name" value="Ribosomal_mS25/mL61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="6">
        <location>
            <begin position="39"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="144"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="196" mass="21740" checksum="6C6D51D6EB644E4E" modified="2022-01-19" version="1">MNVVKRTKKLKALLDIRIGPGAAVLSNDVKRIHLDFAHKLNDGHFGARKVWRKFLPRLKYHNPAISMTVNRTLDQEGPATLTVFFTPPPRSSSATASSAPFSSTDPSTAPSGHAPGAKIETIDMKHKHESEILSRLLELTKGMPYEASPDELAELRDVDDEKRQSERDREAQARLNEIKRQEKAVLDLARGGVEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAL0</accession>
    <name>A0A8H6FAL0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002277</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150857.1">
        <property type="nucleotide sequence ID" value="XM_037293204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAL0"/>
    <dbReference type="GeneID" id="59330691"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050493">
        <property type="entry name" value="FAD-dep_Monooxygenase_BioMet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789:SF215">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="425" mass="46542" checksum="22958508EC8802A2" modified="2022-01-19" version="1">MPLKVIVVGAGIGGLCAATSLRQAGHDVEIFERSSFTGEVGAALVLTPNGSRILSSLGFSFERARACKMVQWDTILGDSMGRVASLDLSMAEQRFGAPLWSVHRVDLHNELLRLATSEDTPGSKPVTLRLGAQVVDASTDGSITLKDGSRHAADLIVGADGLHSVLRGTVLTHDTKASASGLSAFRFLMDTTMLEDDAKLAQVVEARGPGLSLIIDAKETISERHVVWYPCRDGEVQNFVGIHPTRPADHEETETEAMKESMLKEFGHFNPGIVRIISRSSHVKCWPLYVHEPLPTWYNGRVILIGDAAHPMMPFGGQGSNSAMEDAGALGHLFRSVHDPATIEKRLQLFWLVRKDRVSRTQIMASVRAGREKEVEQELKKYADPPGSAVPSTHQERTDHDYNYDVFAKCEEVLLEGQMGEIKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB23</accession>
    <name>A0A8H6FB23_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mid2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002397</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150976.1">
        <property type="nucleotide sequence ID" value="XM_037293323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB23"/>
    <dbReference type="GeneID" id="59330810"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028000">
        <property type="entry name" value="Pma1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14610">
        <property type="entry name" value="Psg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034047251" description="Mid2 domain-containing protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="36518" checksum="56DE7A08D814AFC4" modified="2022-01-19" version="1">MLFCIYMALSLGGFVSAASLNQRQADDFSAENPDDSDTSGLPTGVAASAPVPIANTTAASNGTVKASSLSSAAAVATTASSPLNNTNDPNMCHRTYTTDPEGATDIANPFCKPGDGQDVYPNIPYQVTWDPSLFEDGANNTVQVYNVDATGLNSPATADILASGNATNFEGSVFLNMSSVWLNGQPTANLTLSLIVDKGDDDPKVFGGPIFTLLSTSDSDSTHSAPSSASSSSNKLGEKVGLPVGLVFLIVAAIAVALFVCFRKRRGQGYGTGKSLRQRVGSGTARGHRRDASFHDEPTRGVELKDRNKGLTGESPEDNWDWGSPVSSPSNAGGAGNAFRDEIQRQRSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB80</accession>
    <name>A0A8H6FB80_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001958</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151425.1">
        <property type="nucleotide sequence ID" value="XM_037292886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB80"/>
    <dbReference type="GeneID" id="59330372"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="19265" checksum="5592672E80F1D028" modified="2022-01-19" version="1">MATNRTSDAGQVISEVSQQEGGTYKGSTAAQMQSQTTKQRNLEDAAAKVGSKLDTAPEAVTSEDASLLHSREARVMGGPTKGDLASQAQSVAAANEQGATIQTNPTQSSGTNANLEPAQQSQLDREANYGEAADKVGTKLATEPETVTKEDGDLLHSRETKAFGATKKGGIASQAQSQAAKNAGDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC43</accession>
    <name>A0A8H6FC43_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chitin synthase export chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_001450</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CHS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151799.1">
        <property type="nucleotide sequence ID" value="XM_037292380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC43"/>
    <dbReference type="GeneID" id="59329866"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022057">
        <property type="entry name" value="Chs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329:SF2">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12271">
        <property type="entry name" value="Chs7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6222364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="329" mass="36150" checksum="534E0C1EA52C2770" modified="2022-01-19" version="1">MGFGDFETICRKSALPVCSVVGPTNPISNSTTGIQATCYARNIELANTIIFEAATDFIHILALGMAAIMVLHVRSKFTAVGRKEITTFFYIYMALTMTSLILDAGVIPPGSSTFPYFVAVQNGFTSALCTCLLINGFVGFQLYEDGTRQSVWLLRGSSIGMFVISGAVSILTFKGRAGLGPTNTVGLFVVLYLINGICLLIYLAMQIILVTNTLQDRWPLGDISFGVFFFVIGQVILYVFSDTICDAVSHYLDGLFFATICNLLAVMMVYKYWDSITKEDLEFSVGTKQGNWEVKELLPEDDRRGTIYHDGTSEYASSTYPLRDSNYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCU2</accession>
    <name>A0A8H6FCU2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">GPI ethanolamine phosphate transferase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000183</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGG/PIGN/PIGO family. PIGO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152558.1">
        <property type="nucleotide sequence ID" value="XM_037291123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCU2"/>
    <dbReference type="GeneID" id="59328602"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16023">
        <property type="entry name" value="GPI_EPT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037675">
        <property type="entry name" value="PIG-O_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039524">
        <property type="entry name" value="PIGO/GPI13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071:SF1">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="501"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="548"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="574"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="631"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="706"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="736"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="776"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="834"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="858"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="928"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="971"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1001"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6223341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1039" mass="114408" checksum="E66BDED604820A7C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDS6</accession>
    <name>A0A8H6FDS6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153209.1">
        <property type="nucleotide sequence ID" value="XM_037301575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDS6"/>
    <dbReference type="GeneID" id="59339113"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="477" mass="49986" checksum="81385197CF1A392C" modified="2022-01-19" version="1">MGGQASNQLTVANALEIAKANQNGQIPPAVTQTLERNIGDIWRRIQTQPSTYVMRKEEFAVFTYYRSRYDNNAVAQQAVARFWNHYKGDAPRVDGARSSSSTPSASGGPSSPRGQAGTGFVPRDFASDEAIARRVCEPAAPDVSESSIIANVCNETLANDSPTCSNQMTVHMQQSAANTCGQYFDLFNGCLMANNNDHTACSDSLLGYQDCNNYTVQAYAYCGCYYTLPASLAECANEQLQPQAQYTAMVSPTNAPVTAISTGSDTVAAVASADQEPTPAVLAVSGKAPYPSSQSTVPTDPRTPSAPTLRITSLPLKWAMPSTDLGNAWTPSAARSTSTEPITVVVTYATTLTAAEYTTTVTLTSTSITTVYTCTDGGDVTLPAPVSAASALIGPAPIVSAPRGPETFAPHSAVEPAQSTGARIAEGRTEQTGDQVTSTFPSVAAPALSTGVGPPEATVGRNGSAVCARCRRRSKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDS7</accession>
    <name>A0A8H6FDS7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LsmAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010722</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153208.1">
        <property type="nucleotide sequence ID" value="XM_037301574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDS7"/>
    <dbReference type="GeneID" id="59339112"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045117">
        <property type="entry name" value="ATXN2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009604">
        <property type="entry name" value="LsmAD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025852">
        <property type="entry name" value="SM_dom_ATX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854">
        <property type="entry name" value="ATAXIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854:SF7">
        <property type="entry name" value="ATAXIN-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06741">
        <property type="entry name" value="LsmAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14438">
        <property type="entry name" value="SM-ATX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01272">
        <property type="entry name" value="LsmAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LsmAD" evidence="2">
        <location>
            <begin position="266"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="782"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="826"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1003"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="534"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="597"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="692"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="796"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="834"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="860"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1021"/>
            <end position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1075" mass="115769" checksum="1DE43AACFBFE6239" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDU3</accession>
    <name>A0A8H6FDU3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153269.1">
        <property type="nucleotide sequence ID" value="XM_037301635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDU3"/>
    <dbReference type="GeneID" id="59339174"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000046">
        <property type="entry name" value="JmjC domain-containing protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF101">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="1">
        <location>
            <begin position="275"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="442" mass="49680" checksum="179C73014D7D0667" modified="2022-01-19" version="1">MLPTPLLELLARTLETLYQPCPNDPIFDCPPSTRSILPSRPAALIQLAHAKIHSYPFSKVPVCWRRLFTDASIGQAIRVVKHGVEAIERAGSIEDADRNNVVNGRGEREDWVQEVVRLLDMAVIMAGAPEREEMIEELLSALQIYVEEKDRGPPRKKRRVEGDRFSTEGNNVPEIRKQIPIRAVLSMEAFEKHLNTGLPLVIQGAINHWPALHERPWSSPAYLMKKTFGGRRLIPVEIGRSYTDAEWGQSIITFKDFMSEYLLQDSSNAGDNGIGYLAQHDLFTQVPSLRNDISIPDYCYTNPPPPAPDTPLGVKELQPKLDEPLLNAWFGPAGTVSPLHTDPYHNILSQAVGKKYVRLYSPSESEKLYPKGMEGGGVDMSNTSEVDVEAEEGVLDEGFPLFRGAVYVETILDEGECLYIPVGWWHYVRSLTVSFSVSFWWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE86</accession>
    <name>A0A8H6FE86_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154007.1">
        <property type="nucleotide sequence ID" value="XM_037301190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE86"/>
    <dbReference type="GeneID" id="59338728"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="151" mass="15640" checksum="A2B60220221D79CE" modified="2022-01-19" version="1">MTKRRNATDVSKPPSKPKLSTEVTKIANAITGVAKNPLVEKRKPTTKPKPAKTQNNNNTTTADDVNAASTAVPTAPDVLAIEPPPPVKRTPRPATMKPAAATGAPSGGATGVAKGKKPILARTPPEQQHGGRAKIGRPEFRYGGGDRGAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEM1</accession>
    <name>A0A8H6FEM1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010443</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154113.1">
        <property type="nucleotide sequence ID" value="XM_037301296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEM1"/>
    <dbReference type="GeneID" id="59338834"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="410"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="491" mass="57718" checksum="68A37E6C9F4CA44B" modified="2022-01-19" version="1">MSPTSAPVPVDHHDWNEYDPEALPSFRRPYHYNDIEVPINCERLSISDPPDISDPINSEENDAEIRWCPRCFTGFGSTEALLEHRRTSALHITCEFCERAEDFANEEELWQHCCGHHFACWYREGATFCANVCKSAADLDRHFRIDHFSCRFCIYVQLFATLEDLKDHYRASHTTCPRCPDPLVFRYKEELREHCLREHFGCPCCDSVTWSKDEESLRTHIGLEHFPCCFCISQTVFASREDLLAHRRTAHGCFDCLLCKPQQPRIIWPAPWREHMEQCYYPNPCTDDFHQDGFDMISYCDLCEKTFKPQYRQIHLLFNHFCQDCARYGTIFKMHKEKHLSECSRQGASAGPPWWHRQEQQQQNESARESFRRQHEDFKTRANEQRAHPSASHPHPPPPTQKPQEPEPLDIYTILKISPQSPPEVMKQAVRVRRVETHPDKRKRQGLSQGEEDLIDEEAKLVGWAADILLDPEKRKKHGEQVHAWKMRYGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEQ5</accession>
    <name>A0A8H6FEQ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154496.1">
        <property type="nucleotide sequence ID" value="XM_037300648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEQ5"/>
    <dbReference type="GeneID" id="59338184"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581">
        <property type="entry name" value="D-ALANYL-D-ALANINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="46612" checksum="1242DA7BCD7D4EA9" modified="2022-01-19" version="1">MTSPQRLEGSSTSKRHSRNASYNRAPLARRMTKGPLDSDDPLAVQSPQTPSHVTIEAANIPIPTTSFSPERPFAQPAVTAPPPPPSKDFTYLLRPDIYHPLSQLDLPSQFRTPKRQPPPSTPTPTLLANHNFRSAATAAALRLCTPPAPPPNELFPLLYTRLACLTLISHLPFAAEESKALQDINSPFYRDEVTGKNILPWELRVLAVRLQGVGYGDGRRGVGGYYDLAREARAEVKKAVDPEETRKWQERLKELGLCVANALTEMGDAAAAVRHLESLRPKTGKDEVLEGRLALLYIHVGNVDAARQCLSDTSASDLRPLLSMADGRYADAVEEWNALPSSDLATQNLAVCLFYTGEVDKTLELLDQLVEKGRSFHALTFNLATVYELCSEKGSQKKEELVERVSKRIGETGGGERGVVDFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFM0</accession>
    <name>A0A8H6FFM0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009416</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155103.1">
        <property type="nucleotide sequence ID" value="XM_037300277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFM0"/>
    <dbReference type="GeneID" id="59337811"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38049:SF1">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38049">
        <property type="entry name" value="RICIN B LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034900026" evidence="2">
        <location>
            <begin position="21"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="31157" checksum="04B45F09B693E626" modified="2022-01-19" version="1">MVLGLLAIAAIPTTIGVAEGISEGRKENDPETEEERMRKFQLECFCGANSQKAKEIDGGHIVLRGEKLYIVPRHSSSTSHPFEGFYIEYPDPERPRPLPLGLVSTISKDPPMLNWIYVDKNTRDVKYGNRTQSRAHIVGSWGWDAGEEGGAGGLTLAGGEGAVAVEGDDGWEIRWEDEDGKAGVERRQKLTISLERKMLEPREEDKPDHARASEREDDQQTKRTNTNIELTNTTFQRSKRTTGEPERVDKAVKKGAKENHSAPKLEIRGTTVERPLARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFZ6</accession>
    <name>A0A8H6FFZ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TEA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009310</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154997.1">
        <property type="nucleotide sequence ID" value="XM_037300171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFZ6"/>
    <dbReference type="GeneID" id="59337705"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF0">
        <property type="entry name" value="PROTEIN SCALLOPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="8">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="6">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="110"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="452"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="717"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="454"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6226444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="737" mass="83047" checksum="D17BCEA570CB0442" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG24</accession>
    <name>A0A8H6FG24_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009400</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155087.1">
        <property type="nucleotide sequence ID" value="XM_037300261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG24"/>
    <dbReference type="GeneID" id="59337795"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021138">
        <property type="entry name" value="Ribosomal_eL20_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10052">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="4">
        <location>
            <begin position="68"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="238" mass="27935" checksum="2FEC46C17DF6E42B" modified="2022-01-19" version="1">MHLRRYEGAGKEKPYFQRESKNVYPIVTRRCHGNGYRTQDAGSANNALEDLLRSCAIWVLIEWGSGRLNEYQVIGRHLPTDTNATPKLYRMRIFAPNTVVAKSRFWYFLMKLRKVKKSNGEIITINLIHERRPTKVKNFGIWIRYDSRSGTHNMYKEYREMSRTEAVDALYQDMAARHRARFRSIHILKVVELEKTDDVKRPYIKQLLSKNLKFPLPHRVPKATSKALFAAHRPATFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG31</accession>
    <name>A0A8H6FG31_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155323.1">
        <property type="nucleotide sequence ID" value="XM_037299322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG31"/>
    <dbReference type="GeneID" id="59336852"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="196" mass="22075" checksum="8AF93A27AB7345FF" modified="2022-01-19" version="1">MTREPTLTGRVSSRADPEPDMSSDNEDQATGGRKPQPNQELQKQPPQTQQANRNQSAPSNQDQGLQQQNEALQQQLMALRQQQSQQPEVKGGRIAPVRQSRILDRAPSEGAKNSSLKIQIELDLEVELYTKPEQYIRIRSPKRVDETPFDKRRSGSLWSTPEINWLGILVQDDSPIDEVFSPTTKLSGKIVQYMAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGM5</accession>
    <name>A0A8H6FGM5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008605</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155471.1">
        <property type="nucleotide sequence ID" value="XM_037299470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGM5"/>
    <dbReference type="GeneID" id="59337000"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF6">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08740)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="58690" checksum="773C92AA24BDD59F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGM7</accession>
    <name>A0A8H6FGM7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155714.1">
        <property type="nucleotide sequence ID" value="XM_037299713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGM7"/>
    <dbReference type="GeneID" id="59337245"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="485" mass="53035" checksum="BE9DFF516344337C" modified="2022-01-19" version="1">MSQHLGHSTSRNMVSGRPRPPPELASQQQYLLDPLPDSGSSQSSAPFSQYSLSQPTSQYTMDGNPQEPALAPMQYPPFQQYTGTQAVYQNFRPPYHLTGTTPNGHPVSHQRYSERDCTTSFRGAGGPVFGDDLRNHNGGNSGWSSMGDSSRQNVRGSFIEPCYPNSDGDGYSQAQQHLTEHRTMDQTNALTGRDSCKGFPVQYRIDATSENFGMRVSPSFCSATDNHGAGGDIDNQNCNLATADNNIGHGSQWSDSQEMGNQANPSTIPPSGIDLSLIDGSFSAKYNSFLADLAETDPGGSDAQNEMLLCNPDHFDNSFLNGSPTSTFSGSGTVSRKARSRATSNTTPHSHRRGRGRDRGRGSAHPVSSEPLETRCKACDDDPDCEDKPVYRGTPESQKTSLGRHMREHHSDSKNWSYQCLLDKDGSACKKVIRKARGRRKHVETVHPTESRNLPPKDAAKRNSNVNTNAMLDEWFDKIPQGSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGP0</accession>
    <name>A0A8H6FGP0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_008650</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155516.1">
        <property type="nucleotide sequence ID" value="XM_037299515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGP0"/>
    <dbReference type="GeneID" id="59337045"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000141">
        <property type="entry name" value="C2H2 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000027">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF7">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G04110)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="488"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="370"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="581"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="392"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="448"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="581"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6227208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="620" mass="67317" checksum="6C8DC4FCA903B57B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHK6</accession>
    <name>A0A8H6FHK6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DUF21-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_007898</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156102.1">
        <property type="nucleotide sequence ID" value="XM_037298766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHK6"/>
    <dbReference type="GeneID" id="59336295"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000006">
        <property type="entry name" value="DUF21 and CBS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="11">
        <location>
            <begin position="64"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="329"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="457"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="598"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="460"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="535"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="625"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="705"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6228168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="731" mass="78687" checksum="280E34E699829296" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHQ4</accession>
    <name>A0A8H6FHQ4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156193.1">
        <property type="nucleotide sequence ID" value="XM_037298857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHQ4"/>
    <dbReference type="GeneID" id="59336386"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38846">
        <property type="entry name" value="C3H1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38846:SF1">
        <property type="entry name" value="C3H1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="309" mass="34187" checksum="120D5A9CC2BB9F8F" modified="2022-01-19" version="1">MSIPPTKSPKQLYRDAVPNAKADLGTPPRKISTPSHPIGPTLVNPGVKSKQRQKPSVPFSALYSPPSKPAIFVARQVGTQPARSGAVCKGWKDHEWRPDIEAVGADEFESNKAAASPQILVPNFASPYENSFPQLPALLIIPHLPVGTMAEQAYFGRFQTFAPDPQATVLENFAQLAISNGWGKKSQEYKDERKSYMIALADTHIESIERGGAAGTLAGLQGLCKELRVCPIPTSITQCKKKLRTVHVCIVDLIDNRRLGGTRVHIFPCHRELQQHIKKRKHYFPKYEAKEKEGGLLKVLLRKIKGYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKS3</accession>
    <name>A0A8H6FKS3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004662</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157579.1">
        <property type="nucleotide sequence ID" value="XM_037295578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKS3"/>
    <dbReference type="GeneID" id="59333069"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00322">
        <property type="entry name" value="FNR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052128">
        <property type="entry name" value="Oxidoreductase_NAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46505">
        <property type="entry name" value="OXIDOREDUCTASE NAD-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46505:SF1">
        <property type="entry name" value="OXIDOREDUCTASE NAD-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="3">
        <location>
            <begin position="45"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="327" mass="37146" checksum="7ED26C7E682D9403" modified="2022-01-19" version="1">MGSRLRALRSLSILARLPLQDITPRRAMTSKTSHEERTAADRREPGLHTVVLDKIEQVNMNIRLLKLVPRGEKPKFLPGQWLDVHVPRIPQAGGFTITSTPDDLKKDEPYLELAVQHSPNNPPAAWLWQREKKILGTELQIRVGGSFVWPPPGIDTNQVHKVVLVAGGVGINPLLSIITHLILHSDYHGQIELLYTSRKGTSGNISSILFVKRIHELFQRDLPQKRHHYTLFCTAPFALPSTSHSGKKRLNVIRERSGDMHYVGEHQSIEYRRFEQKDLETALGPVEERGNTVAYVCGPPRMTDWAVDVLNRSKGMDQARVLCEKWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKV7</accession>
    <name>A0A8H6FKV7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157652.1">
        <property type="nucleotide sequence ID" value="XM_037295651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKV7"/>
    <dbReference type="GeneID" id="59333143"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="126" mass="14077" checksum="3882E225BE8DF156" modified="2022-01-19" version="1">MHSGAVYPQDFPKSGFNDYELLDRTLETMVTRKRLNVEAPRRSPAAGPLGVAKKVDLVIEPAAWKKHGTVIVVDYEAWIMNLWLLRTDIAGKGSLDPDYEPVVLLPKQWVKPGYGGDVKSIVGNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL39</accession>
    <name>A0A8H6FL39_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157751.1">
        <property type="nucleotide sequence ID" value="XM_037295750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL39"/>
    <dbReference type="GeneID" id="59333244"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="167" mass="18814" checksum="A58794F26CD353AB" modified="2022-01-19" version="1">MPAILQLGSMAKQPFADAATEFAQPSVQSPSVGNQQLPEAKPEETPPSNIKKLVRKLTKRVKPSKEKGASLPAENESAEDKLVGFISRNPQLFVPAEEERKQASLQIPEIVVRARTLSRSQRQLERLENPLILDATPVEEADEQEKAKETMRQSRTARFRKRLEEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYK0</accession>
    <name>A0A8H6KYK0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial carrier protein pet8 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156813.1">
        <property type="nucleotide sequence ID" value="XM_037298178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYK0"/>
    <dbReference type="GeneID" id="59335686"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13191" checksum="CC87D463CCE44EA8" modified="2022-01-19" version="1">MSFITRIALRSRASPLRLAPISTRPLHVTSARAVLSESDHGGHHDAEDRKAKIDHHKDDQLNKQKDGKGHWKRELGSNSESAVKADREEITDAEHDVESLQKETSEAMEDDHEHAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZS6</accession>
    <name>A0A8H6KZS6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004516</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="3">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157434.1">
        <property type="nucleotide sequence ID" value="XM_037295433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZS6"/>
    <dbReference type="GeneID" id="59332924"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="5">
        <location>
            <begin position="292"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="165"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="654"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="880"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="731"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="755"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="768"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="820"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="850"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="887"/>
            <end position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1010" mass="110495" checksum="7B436B342E965488" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5I3</accession>
    <name>A0A8H6C5I3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Adenylyltransferase and sulfurtransferase uba4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Common component for nitrate reductase and xanthine dehydrogenase protein F</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin-like protein activator 4</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="14">Molybdopterin-synthase adenylyltransferase</fullName>
                <ecNumber evidence="14">2.7.7.80</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="14">Adenylyltransferase uba4</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="14">Sulfur carrier protein MOCS2A adenylyltransferase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="14">Molybdopterin-synthase sulfurtransferase</fullName>
                <ecNumber evidence="14">2.8.1.11</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="14">Sulfurtransferase uba4</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="14">Sulfur carrier protein MOCS2A sulfurtransferase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="14" type="primary">uba4</name>
        <name evidence="14" type="synonym">cnxF</name>
        <name evidence="17" type="ORF">HO133_006863</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and MOCS2A. Its N-terminus first activates urm1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and mocs2a. Its N-terminus first activates urm1 and mocs2a as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and mocs2a to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards urm1 and mocs2a. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:43616"/>
            <dbReference type="Rhea" id="RHEA-COMP:12159"/>
            <dbReference type="Rhea" id="RHEA-COMP:12202"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:90778"/>
            <dbReference type="EC" id="2.7.7.80"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48612"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12159"/>
            <dbReference type="Rhea" id="RHEA-COMP:19907"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:232372"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.80" evidence="14"/>
    <dbReference type="EC" id="2.8.1.11" evidence="14"/>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5I3"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061605">
        <property type="term" value="F:molybdopterin-synthase adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061604">
        <property type="term" value="F:molybdopterin-synthase sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042292">
        <property type="term" value="F:URM1 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
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            <begin position="425"/>
            <end position="534"/>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5W1</accession>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">WasteWater1</strain>
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        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="565" mass="63787" checksum="E35BDC86E36123E4" modified="2022-01-19" version="1"> </sequence>
</entry>
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        </lineage>
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    <reference evidence="2 3" key="1">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="144" mass="16788" checksum="9434EE8FA6D648E4" modified="2022-01-19" version="1">MGRKRSRKKPRRKNKVYDTPKKAKVQGAGIPEDPRNVFEFFDVEQRPGYKMIESDAPSRTFHNQGHNETRGRKHKMTGAQVAQVSQLLEDTDLGMKAKGMNWYAIIWELDLDIKSPKTVRDTMCDAFDYGKYKTTLKQFMDQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8H6C704_9LECA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="1" type="ORF">HO133_006479</name>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037297377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C704"/>
    <dbReference type="GeneID" id="59334880"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="140" mass="15191" checksum="188BF542D9449327" modified="2022-01-19" version="1">MLHAVVENTSFGGGSDAYQGKCGFEAFTHHRPAVPVPGWFECLCSFRYPPYHKKHMSKVDVKNPSLTRGETLEDQKVGSSTATTVSKLLATSAKWATVAVNFAMVETRIEMLRSAIGGLRNGLTRGLHPLLVREADGTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C708</accession>
    <name>A0A8H6C708_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Long-chain-alcohol oxidase</fullName>
            <ecNumber evidence="5 12">1.1.3.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_006370</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Long-chain fatty alcohol oxidase involved in the omega-oxidation pathway of lipid degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>a long-chain primary fatty alcohol + O2 = a long-chain fatty aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:22756"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17176"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="EC" id="1.1.3.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.20" evidence="5 12"/>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147393.1">
        <property type="nucleotide sequence ID" value="XM_037297268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C708"/>
    <dbReference type="GeneID" id="59334771"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046577">
        <property type="term" value="F:long-chain-alcohol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012400">
        <property type="entry name" value="Long_Oxdase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46056">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46056:SF12">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028937">
        <property type="entry name" value="Lg_Ch_AO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="14">
        <location>
            <begin position="270"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase C-terminal" evidence="15">
        <location>
            <begin position="590"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR028937-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6217958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="752" mass="81331" checksum="2E44EE1F28BFFDD7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7I2</accession>
    <name>A0A8H6C7I2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005734</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147822.1">
        <property type="nucleotide sequence ID" value="XM_037296641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7I2"/>
    <dbReference type="GeneID" id="59334139"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="3">
        <location>
            <begin position="427"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="427"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="364"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="485"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="512"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="543"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="624"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="679"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="708" mass="76732" checksum="5F406BCB05263379" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7Q4</accession>
    <name>A0A8H6C7Q4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006143</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147619.1">
        <property type="nucleotide sequence ID" value="XM_037297045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7Q4"/>
    <dbReference type="GeneID" id="59334547"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF1">
        <property type="entry name" value="INHIBITOR OF NUCLEAR FACTOR KAPPA-B KINASE EPSILON SUBUNIT HOMOLOG 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="47"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="657" mass="73697" checksum="EBDC80F0CB11DCEE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7S4</accession>
    <name>A0A8H6C7S4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147649.1">
        <property type="nucleotide sequence ID" value="XM_037297075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7S4"/>
    <dbReference type="GeneID" id="59334577"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="150" mass="16235" checksum="03AEE682829359C7" modified="2022-01-19" version="1">MSSGQYVSAVFGPAKRRAGSQKDEESSLPMSAGRAKACTACRQVKKELMDALSKLEEVTSELTSLKEAFKSQSTPRNPNPNQVGGSFPNSHSPEAGLLGLEATRPTSVEVQVPSFFGIEIFEQSAFEIGEQFLGDIRLSGMAIVQLFEQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8C3</accession>
    <name>A0A8H6C8C3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA methyltransferase 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005835</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147921.1">
        <property type="nucleotide sequence ID" value="XM_037296740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8C3"/>
    <dbReference type="GeneID" id="59334240"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18105">
        <property type="entry name" value="SpoU-like_MRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000035">
        <property type="entry name" value="TrmH family RNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047182">
        <property type="entry name" value="MRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047261">
        <property type="entry name" value="MRM1_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103:SF1">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="11">
        <location>
            <begin position="253"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="134"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6218486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="578" mass="64156" checksum="8663982E1F11A908" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8F5</accession>
    <name>A0A8H6C8F5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148302.1">
        <property type="nucleotide sequence ID" value="XM_037296320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8F5"/>
    <dbReference type="GeneID" id="59333816"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="289" mass="30129" checksum="D30618FF6BF117D3" modified="2022-01-19" version="1">MANSTKSAIGVDNRVSSTFTNGFFLGVATVVTTITGPSPMPVTTSSETGATSAAVSTAAYPCPDLDGTSYTDATGNNYKIQCSTNYPGNDLPAVHADTFEECLRVCDAYVPEPSAASEASCIGVSWGAGNPGGNCYPKYQITTINTNDGGLSSGYHVNYTLPKSAVTNQGNSLSILNAPATSSTHSATSTIMRTPTPSGSSDGHAAVGVSAGVGVLVPVGLAVFAGFFYLIRRRSKRRPEGLLDVHAVANEKNDNGLREQRAERRLDELPAENLPGELSVPHRPRAELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8G2</accession>
    <name>A0A8H6C8G2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_006044</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GTP-binding elongation factor family. LepA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148128.1">
        <property type="nucleotide sequence ID" value="XM_037296947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8G2"/>
    <dbReference type="GeneID" id="59334449"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03699">
        <property type="entry name" value="EF4_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01890">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000078">
        <property type="entry name" value="Elongation factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000015">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000007">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2570:FF:000001">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000004">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="entry name" value="Elongation factor 4, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512:SF7">
        <property type="entry name" value="TRANSLATION FACTOR GUF1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="TRANSLATION FACTOR GUF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="57"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="66"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="130"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="184"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="653" mass="72783" checksum="DFE2890C21EB0B4F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8T7</accession>
    <name>A0A8H6C8T7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148447.1">
        <property type="nucleotide sequence ID" value="XM_037296465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8T7"/>
    <dbReference type="GeneID" id="59333962"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="22848" checksum="0BC21CB1CE79CC0E" modified="2022-01-19" version="1">MASYSSSNTEPERWAKIVAGVDSGRHPEIMINEEGTLVAVSMPSAKGARIQSFLADVFKDGDNEWFSAVGDSVEASKGKAADYGQPESSKKKKVSLTQQRANRRQEKKDKGQVMQRAEETRESYSTAEWARIMGEASTERPQGVYIEGKKLVATDHTTLDASRKLELFIAHALRHDTTGRGTVVLLRSVGARLLNRSLDKEFSVGMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8W5</accession>
    <name>A0A8H6C8W5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Ribokinase</fullName>
            <shortName evidence="12">RK</shortName>
            <ecNumber evidence="2 12">2.7.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_005554</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose + ATP = D-ribose 5-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:47013"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="2 12"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148445.1">
        <property type="nucleotide sequence ID" value="XM_037296463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8W5"/>
    <dbReference type="GeneID" id="59333960"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004747">
        <property type="term" value="F:ribokinase activity"/>
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        <property type="term" value="P:D-ribose catabolic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7 12 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 13" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="5"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
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        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="13"/>
            <end position="15"/>
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            <begin position="41"/>
            <end position="45"/>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <begin position="234"/>
            <end position="239"/>
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            <dbReference type="Pfam" id="PF00294"/>
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        <source>
            <dbReference type="EMBL" id="KAF6219010.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="349" mass="37319" checksum="14E9AB5CF68D2C8C" modified="2022-01-19" version="1">MPTRNITIIGSLNTDLITRTSRVPAAGETLITSSYDTGCGGKGANQAVACARLSREKGSEVDEAGCTINMIGAVGDDMFGRDLINGLVSNGIGVDGVRVKDGEKSGVAVIIVEEESGENRILMSPNANYALRPSDFKSLPDPLPDLIILQLEIPLITVLHILKLAREKNVEVLLNPAPAVKLPREAYLAVNHLIVNETEASIITSTTGQKHSWALWGEHIQRLIDLGVRHVLVTLGAEGVIYLDTGIQRVLQAPGEKVTVIDSTGAGDTFVGAYAGMLAKSGRDSRDFTCMMELVDFANRAAAKTVQKGGVQSAIPWRNEVSLENTLAFAGSGISFDEWKKGVDPAFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8X2</accession>
    <name>A0A8H6C8X2_9LECA</name>
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        <recommendedName>
            <fullName evidence="7">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005329</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="94"/>
            <end position="126"/>
        </location>
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            <begin position="164"/>
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        </location>
    </feature>
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            <begin position="181"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="19781" checksum="321C7FDB08EED94D" modified="2022-01-19" version="1">MPTATLSTITTDQIDDLLYLARLGETTDLTAGVNLFAKSLSATPGIILTAAIDKDSGNGLLHMAAANGHTDTLKELLTLSASNHALSLNLQNASGNTPMHWAALNGHLPAVKLLIEAGADPTVINKAGKDAVYEAEANEKNELASWLLTEGKGLESAVGGSLNQIAPGVGGEGDAEGQLEEVMKEGGTEEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Z1</accession>
    <name>A0A8H6C8Z1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Telomeric single stranded DNA binding POT1/Cdc13 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148316.1">
        <property type="nucleotide sequence ID" value="XM_037296334.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333830"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04497">
        <property type="entry name" value="hPOT1_OB1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011564">
        <property type="entry name" value="Telomer_end-bd_POT1/Cdc13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02765">
        <property type="entry name" value="POT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00976">
        <property type="entry name" value="Telo_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomeric single stranded DNA binding POT1/Cdc13" evidence="2">
        <location>
            <begin position="1111"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="830"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="873"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1003"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1262"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1338"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="673"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="830"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="873"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="923"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1006"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1276"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1339"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1374"/>
            <end position="1386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1386" mass="151634" checksum="4A02E5BF366C6180" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9L6</accession>
    <name>A0A8H6C9L6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148852.1">
        <property type="nucleotide sequence ID" value="XM_037296154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9L6"/>
    <dbReference type="GeneID" id="59333649"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="31252" checksum="35838237A866B720" modified="2022-01-19" version="1">MSNRDDIIQSLSRDLPKNGIREISPAGPETLDLCDLYLPHAQAVYGNGQYLAKEDLSQADLVEKLSWYFKTRGSYHTAVALAEEAVRSRKEANYLMSVENLNECSRAGKSGIPTGKKPLGFDHQLTLRSLSNLTASLRFAGEWEAVREIHQRMLSTKEKTLGADHPSTESTGEILSSIEQNLGCYKKEPDGDKKVRGFSMFARYKTTRRKAWAPVMMFVTKKSMRNRVAVVKSLKRRAGPEHPRTVKAREALWSALRRSGAEQEAERELGSYSGNTKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9R9</accession>
    <name>A0A8H6C9R9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005016</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148626.1">
        <property type="nucleotide sequence ID" value="XM_037295928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9R9"/>
    <dbReference type="GeneID" id="59333422"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048685">
        <property type="entry name" value="COG3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048320">
        <property type="entry name" value="COG3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007265">
        <property type="entry name" value="COG_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302:SF8">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20671">
        <property type="entry name" value="COG3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04136">
        <property type="entry name" value="COG3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 N-terminal" evidence="10">
        <location>
            <begin position="139"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 C-terminal" evidence="11">
        <location>
            <begin position="305"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="514"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="856" mass="97387" checksum="380C6ABCA8A4F391" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAA5</accession>
    <name>A0A8H6CAA5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RlpA-like double-psi beta-barrel-protein domain-containing protein-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149199.1">
        <property type="nucleotide sequence ID" value="XM_037294511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAA5"/>
    <dbReference type="GeneID" id="59332000"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF28">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034833441" description="RlpA-like double-psi beta-barrel-protein domain-containing protein-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="15801" checksum="4B73DB8DE72B24C9" modified="2022-01-19" version="1">MLLSYTFVGLYLAAQSFAVPAASGEDASLERRVTHCGDATYYTQGGNAGSCGQTNPDSAYIIALSNYWQDNESPGPYCGRKIQITNQGGGDDNNGQGKVVVATVQDTCPSCDENHLDLSEGAFQALTGGSLDPPGEFNICWHFCNADGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAB4</accession>
    <name>A0A8H6CAB4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1348-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149029.1">
        <property type="nucleotide sequence ID" value="XM_037294982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAB4"/>
    <dbReference type="GeneID" id="59332472"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009783">
        <property type="entry name" value="DUF1348"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31757">
        <property type="entry name" value="SLL0781 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31757:SF0">
        <property type="entry name" value="SLL0781 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07080">
        <property type="entry name" value="DUF1348"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17990" checksum="9764E700136B4DFE" modified="2022-01-19" version="1">MAQHKPPFTLETAHQKVKAAQALWNTRDPSKVARAYTPTSIWRNRSTFLRGHAAIEDFLTKKWEKEKEYRLRKELFAQQDNRIGVQFWYEYRDAHDSMVWKRCYGIEHWTFAADGKMEKRMMSGNDVVIGEEERWFRDGVDVDSVALGEADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB19</accession>
    <name>A0A8H6CB19_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149678.1">
        <property type="nucleotide sequence ID" value="XM_037294297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB19"/>
    <dbReference type="GeneID" id="59331786"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06869">
        <property type="entry name" value="PX_UP2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047168">
        <property type="entry name" value="LEC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024554">
        <property type="entry name" value="LEC1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024555">
        <property type="entry name" value="PX-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12825">
        <property type="entry name" value="DUF3818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12828">
        <property type="entry name" value="PXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="221"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="825"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="867"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="900"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="937" mass="106311" checksum="862742CB8B1E0D14" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBK6</accession>
    <name>A0A8H6CBK6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002869</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149872.1">
        <property type="nucleotide sequence ID" value="XM_037293794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBK6"/>
    <dbReference type="GeneID" id="59331281"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904679">
        <property type="term" value="P:myo-inositol import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000073">
        <property type="entry name" value="MFS myo-inositol transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF22">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="316"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="42"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6220437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="532" mass="57212" checksum="75E55FD8AED33F9D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBN1</accession>
    <name>A0A8H6CBN1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM3</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_003023</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150025.1">
        <property type="nucleotide sequence ID" value="XM_037293947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBN1"/>
    <dbReference type="GeneID" id="59331435"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17754">
        <property type="entry name" value="MCM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000008">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008046">
        <property type="entry name" value="Mcm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056575">
        <property type="entry name" value="WH_MCM3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF46">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23191">
        <property type="entry name" value="WHD_MCM3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01659">
        <property type="entry name" value="MCMPROTEIN3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="302"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="673"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="692"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="708"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="736"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="753"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6220590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="873" mass="96247" checksum="8DD0E119A6175198" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCT5</accession>
    <name>A0A8H6CCT5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VPS37 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_002549</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150304.1">
        <property type="nucleotide sequence ID" value="XM_037293475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCT5"/>
    <dbReference type="GeneID" id="59330962"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678:SF2">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51314">
        <property type="entry name" value="VPS37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="8">
        <location>
            <begin position="158"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="247" mass="27836" checksum="BD35E472B2592D9D" modified="2022-01-19" version="1">MASSALTPLSSYTNPSSAPPPPPKPPQSIPPTPSYTPSYTPTTTGPPRPPQTPSHTTTTPAPPPQSSQPTNPPPPNPSEHWLPDILLDKPSQTLHAALQNEPLLRSLSLLHPSLTPSPLPPHLQQTQNLARHLTAQRAHLLALRQRTQSQLLRLHALERQWREKQALMDRELAPWSAKVLYQRLREGEREGEEWCEGLEGSWLDGEGEVGEREVGEFLRGYREGRVKVWRRKEARERWDEGRVGGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD00</accession>
    <name>A0A8H6CD00_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002707</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150461.1">
        <property type="nucleotide sequence ID" value="XM_037293632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD00"/>
    <dbReference type="GeneID" id="59331119"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF68">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="3">
        <location>
            <begin position="217"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="815" mass="91613" checksum="0A585B21EB9E3F90" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD06</accession>
    <name>A0A8H6CD06_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002627</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150381.1">
        <property type="nucleotide sequence ID" value="XM_037293552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD06"/>
    <dbReference type="GeneID" id="59331039"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40623">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40623:SF2">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="468" mass="52190" checksum="5D056E7AD6D412E3" modified="2022-01-19" version="1">MGKAFFTSWVLWEKMVFILGASILLVIFLGLIKLTYNKWRLRKYTAVAETKAAVRREMQHTASVKRGRSQRQGIPFGIRALESGIEVDGVWISGANTPSSMPGSPNIAAMKPKPARQDHSPEASSSTSEMSRIEIPQPVHGYPNNPRMNHSAGPSYKLPTPIDRPVASEQQHIKPPTSDHQSHGRPTYQPRRSSQLRYSDSLNPEDSEAFAALEGRTMAADRNGKRPEGSDSSGEEYRDSTSWPGHSKNSSGSENHSDANQPIQPKRSSNSYLHPASRQPASRQPPKIRTSNSYNPDRYKPVQLDPLASNTESHMDELGRLHVRNRESGMTVVYAPVEDSVSRRIASSQGPTHEVSDPFATPETSPKEDRIPVMVDAPPANQHSEMIGNGDIDYIQGHAQPLRPFDGNRQLRRSQVIRKVNSGFEILRPGTLVTPRRSSDIADVREDYDRKPKKLQRRSRASSFVEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDH6</accession>
    <name>A0A8H6CDH6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150701.1">
        <property type="nucleotide sequence ID" value="XM_037293048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDH6"/>
    <dbReference type="GeneID" id="59330535"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="149" mass="16563" checksum="32DEE6183F1D31FC" modified="2022-01-19" version="1">MRSLLANDGKLLKQETVDHMFEPQLDDDSRKALMEKLQVPAVNRVYGALPSTAQKNRAFAGLMNCEDIKDRRQAGAITCIGMLNVNWIHHLDKKLLVFVKLMATDVVRSGLTGQLVCVAYVLPNAFHPEMPNASGSVRRLREKCTADTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDI3</accession>
    <name>A0A8H6CDI3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal RNA-processing protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002265</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150845.1">
        <property type="nucleotide sequence ID" value="XM_037293192.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330679"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012978">
        <property type="entry name" value="HEAT_RRP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057860">
        <property type="entry name" value="HEAT_RRP12_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052087">
        <property type="entry name" value="RRP12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25772">
        <property type="entry name" value="HEAT_RRP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08161">
        <property type="entry name" value="RRP12_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRP12 N-terminal HEAT" evidence="6">
        <location>
            <begin position="40"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="RRP12 HEAT" evidence="5">
        <location>
            <begin position="359"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1018"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1172"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1037"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1102"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1178"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1221"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1244"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1271"/>
            <end position="1282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1282" mass="140353" checksum="0EE4978A3C7440E7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDK7</accession>
    <name>A0A8H6CDK7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centrosomin N-terminal motif 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002358</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150937.1">
        <property type="nucleotide sequence ID" value="XM_037293284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDK7"/>
    <dbReference type="GeneID" id="59330771"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012943">
        <property type="entry name" value="Cnn_1N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07989">
        <property type="entry name" value="Cnn_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosomin N-terminal motif 1" evidence="5">
        <location>
            <begin position="84"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="176"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="315"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="485"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="656"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="696"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="732"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="386"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="490"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="505"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="522"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="576"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="656"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="759" mass="83463" checksum="6B580E588CBAE09F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDU4</accession>
    <name>A0A8H6CDU4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002279</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ATP = O-phospho-L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23052"/>
            <dbReference type="Rhea" id="RHEA-COMP:13689"/>
            <dbReference type="Rhea" id="RHEA-COMP:13690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150859.1">
        <property type="nucleotide sequence ID" value="XM_037293206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDU4"/>
    <dbReference type="GeneID" id="59330693"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16929">
        <property type="entry name" value="HATPase_PDK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000065">
        <property type="entry name" value="[Pyruvate dehydrogenase (Acetyl-transferring)] kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF3">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="302"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="109"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="436" mass="49614" checksum="2E2610162EF910C1" modified="2022-01-19" version="1">MSWKKSERLMDTIKHYSGFPATGVSLRQMVQFGERPSTGTLFRASQFLSEELPIRLAHRVQELGDLPDGLNDIPSIQKVQDWYSQSFEDITTLPRPNLPSDVRERLLRPMKQNGRDSKILSQTTHNPSVKQGQYRSQQTNGNSNGGGKGSSRRYYASTDDEGDWPPELNDYNKRFSRTLEGIKRRHDSVVTTVAQGILEYKRKRQRMQIDNNIQSFLDRFYMSRIGIRMLIGQHIALTDQKQAIEPNYVGIICTKTNVRELAQEAIDNARFVCEDHYGLFDAPKVQLVCKADLHFMYVPGHLSHMLFETLKNSLRAVVETHGQDKQEFPVTKVIIAEGKEDITIKVSDEGGGIPRSSIPLVWTYMYTTVETTPNLDPDFNKSDFKAPMAGFGYGLPISRLYARYFGGDLKLISMEGYGTDVYLHLNRLSSSSEPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEM0</accession>
    <name>A0A8H6CEM0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001946</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151413.1">
        <property type="nucleotide sequence ID" value="XM_037292874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEM0"/>
    <dbReference type="GeneID" id="59330360"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="292" mass="32373" checksum="0351997264BBB041" modified="2022-01-19" version="1">MSDIVQFKIGKTRARLVAQWQEMIVLSSVNNPAHGNEGLRRTASLLFNPLLNFYTAAGSTRDVAASISSVLNTGGYVAGLSNTDTMEKKNTYIDNESLEVSLVQDPDIVRGCFDQGTLKTYVTLPPVALNMPSIVRLCPGFLLQEAFDWKLAPTTPVRKCQRLWDDPKGWLSSSKAKTVVDLAVAKDPYYEYERALLQTRPSNLNNGGVTRVPFFQKIVEVIKTPDTDGWKAWRKNLGSDLVVPAAVAALSTLMEQQRYCRQTPEADGTFQPSPGTQQGWITWVSDADPDWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEY3</accession>
    <name>A0A8H6CEY3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151644.1">
        <property type="nucleotide sequence ID" value="XM_037292225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEY3"/>
    <dbReference type="GeneID" id="59329711"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="420" mass="46494" checksum="AB4861E65FEB8A97" modified="2022-01-19" version="1">MASTFPMPSTDYGVMLQDVSNYPSRSQFLRRLSTHSSSTSGSNKRAPARITKQNSAGNSPHNVQRRRTTANTARTYPPLSQSSPYQTQEQSLRNYYSSRQAMPVERPMSWHPGSEMISTPATQVHLGEPGLGNTIAGLQNLAVCTRPASSVQQSIENAFAVGYGIPISRPLTMDEHSPTGIEDYASAPDSVYRPESLYHSEPIYNPYSYDAFQQLQHSYMPQSSNQDTYPPANYQIPQWPEAQPFFAHDFQDSQTSAGLCQWSSNPGQSLNIKAAPQVSKRANKVLSGIGLYDDKVPDSVSGTSGDPNRDSMGKGLKLEETWQPPKDEDENDDDDDDDDEGSCSTDEAEEMEEDLPIMTSAPQHAQTAFYPPYGDLSNQSFFFSNDDDAYTGDDQYTNYLAFGQAQSKPQDPVTGNFLWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEY8</accession>
    <name>A0A8H6CEY8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151765.1">
        <property type="nucleotide sequence ID" value="XM_037292346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEY8"/>
    <dbReference type="GeneID" id="59329832"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="497" mass="49923" checksum="A364A6D69B79F771" modified="2022-01-19" version="1">MPSTPNGLGRFENPLRYIAGKKRPDKADKPGGGAQEKNKEGEKLKPKPKQGGKGEGGRKVIVRSSRGYDVKRLPQKDGGCGGGREGGGGAGKGAKDRPRGSGRVGSGGRGEGGGAGSVRRGGGDGGGGDGGGDGRGGDAGGEGAGGGDAGGGDAGGEDAGGEDAGGGDTRAEDAGAEVAGGEDAGAQDTRAEVAGAEVAGAEVAGAEVAGGEDAGAQEAGGGDAGGEEADGQDAGGEDAGAQDNRGEDAGAQDDGGEDAGGGDAGEPDAGGQDNHGEDVDGQDAGGEDTHAEDTGGEDTDGENTLVGDDGGEDARGEISSLAMSTHVPLPIAAPSPPQPASGEDMNKQRRDRVVVIPGRRADTDSEEGNAKEESREGEDEEGEANEHHQEDCEDEKGEANEIDEEDEECDVTYNATCVVVVKSFDYRSACVKKASKTHLRHRFETLYEPESHISAKSEVMNVAAEAMVTMIEEQTAQNEYQPSYHTMFVDDLLGWCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFL6</accession>
    <name>A0A8H6CFL6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152019.1">
        <property type="nucleotide sequence ID" value="XM_037291657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFL6"/>
    <dbReference type="GeneID" id="59329138"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="300" mass="33674" checksum="3419A2E92D579B54" modified="2022-01-19" version="1">MFRYSDASGHRASYASYCGHFYIEWPLGAAAVVQFHPHDSHDTSTDVYPLTFRVRVSKCVQMMAGVITSADGTSFQCDFPGRKPPATWILEYQRKGFPGAHGSRHLYNLMGGKVYDADFLLARRMDEERPSGAIDLRLPSTEQNIQVSMLVPTMEQEILEHTLDCLPWSSLSWSIHRGLRDILVAYAKPTMDELRPNIASRLREFINEQPQRLEANGWSAEFVRGAMAELAASSVLAGSGNSGDSVRVVTDAALLLSDKDSSVLDETNFWRKLTQFEQRIGAINECNHRAYEVFCVGMEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGH1</accession>
    <name>A0A8H6CGH1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152152.1">
        <property type="nucleotide sequence ID" value="XM_037291790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGH1"/>
    <dbReference type="GeneID" id="59329273"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021842">
        <property type="entry name" value="DUF3435"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37535">
        <property type="entry name" value="FLUG DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37535:SF3">
        <property type="entry name" value="FLUG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11917">
        <property type="entry name" value="DUF3435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="578" mass="66234" checksum="336A3506A6EF9AF8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHL6</accession>
    <name>A0A8H6CHL6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Polyketide synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_000216</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152591.1">
        <property type="nucleotide sequence ID" value="XM_037291156.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59328635"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049492">
        <property type="entry name" value="BD-FAE-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041068">
        <property type="entry name" value="HTH_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF21">
        <property type="entry name" value="NON-REDUCING POLYKETIDE SYNTHASE AUSA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20434">
        <property type="entry name" value="BD-FAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22621">
        <property type="entry name" value="CurL-like_PKS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18558">
        <property type="entry name" value="HTH_51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="10">
        <location>
            <begin position="386"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="11">
        <location>
            <begin position="1298"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="9">
        <location>
            <begin position="1672"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="9">
        <location>
            <begin position="1782"/>
            <end position="1859"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="7">
        <location>
            <begin position="1298"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="7">
        <location>
            <begin position="1448"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1611"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1752"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1631"/>
            <end position="1660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1769"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="7">
        <location>
            <position position="1332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="7">
        <location>
            <position position="1513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6223374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2513" mass="273415" checksum="94EBA0CD986F7994" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHM4</accession>
    <name>A0A8H6CHM4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E3 ubiquitin ligase complex SCF subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000353</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Controls sulfur metabolite repression, probably by mediating the inactivation or degradation of the metR transcription factor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SKP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152727.1">
        <property type="nucleotide sequence ID" value="XM_037291292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHM4"/>
    <dbReference type="GeneID" id="59328772"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin ligase complex SCF subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="6">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="5">
        <location>
            <begin position="115"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="165" mass="19008" checksum="5D8E6DF4BE93142E" modified="2022-01-19" version="1">MAEMQKIKLMSSDSAEITVHRDVAERSVLIKNMMEDIGEQAMTEAIPIPNVNEAVLNKVIEWCVHHKGDPPATNDDDADSRKKTTDIEEWDQKFMQVDQEMLFEIILAANYLDIKALLDVGCKTVANMIKGKSPEEIRKTFNIQNDFTPEEEDQIRRENEWAEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI82</accession>
    <name>A0A8H6CI82_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_010506</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 12"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152992.1">
        <property type="nucleotide sequence ID" value="XM_037301358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI82"/>
    <dbReference type="GeneID" id="59338896"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.70.20:FF:000001">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="14">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="775"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="861"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6223932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="911" mass="101962" checksum="B0C46E11F1D01416" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIR4</accession>
    <name>A0A8H6CIR4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010900</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153383.1">
        <property type="nucleotide sequence ID" value="XM_037301749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIR4"/>
    <dbReference type="GeneID" id="59339290"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034520991" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="24"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="537"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="606"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="651" mass="73163" checksum="72DFAFFF6F33D89C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ44</accession>
    <name>A0A8H6CJ44_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153161.1">
        <property type="nucleotide sequence ID" value="XM_037301527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ44"/>
    <dbReference type="GeneID" id="59339065"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="592"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="676"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="733"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="777"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="786" mass="85775" checksum="C5866BF661D9EBB9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ58</accession>
    <name>A0A8H6CJ58_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_011051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153534.1">
        <property type="nucleotide sequence ID" value="XM_037301900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ58"/>
    <dbReference type="GeneID" id="59339441"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07730">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978:SF5">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="53"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="375" mass="41173" checksum="13221229441FE4B5" modified="2022-01-19" version="1">MAPTVKPPPQLNIPDSKSVVNVHIIDSTSRIKGIPFSAFMTPPYKGLDYLDCPAFCFLIENQSSGRKVLFDLGVRKDWENLPATIVKRIKDGGWTLTIKKEVAEILLEGGVAPKEVEAIIWSHYHWDHTGDPFTFPTSTDLIVGPGFKHAFVPGFPENLEAPISKSAYEGRNLREISFADSKLVLGRCKAVDFFGDGSFYLLDTPGHAIGHMCGLARTTVSPETTFILLGGDCAHHGGEYRPTQYLPLPETLSPSPIPRTHPGICPGAVFADIHHLHPSSSSSTEPFLLASEDAAHDIKAARDSVVKLGDFDAHENILTMIAHDDTMINVVGTFPNTLANDWKSKGWREKGMWKFLGDFAEAVEEKEGKEGGKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ88</accession>
    <name>A0A8H6CJ88_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_011090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153573.1">
        <property type="nucleotide sequence ID" value="XM_037301939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ88"/>
    <dbReference type="GeneID" id="59339480"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="463" mass="50750" checksum="A952AD19C268BAC6" modified="2022-01-19" version="1">MEGSVVRGMRPDPDRLPNDHFIEWLAEAKQLYHHELPPLAEKNQGSTRAGLRKPWLGPAEGFSKRTIAYGMWQKDPGDSEGSNIDSKSQHANPMQMLSNPTFVGSPANLDGRAQQQPEWQPSKLHLTNPLDPSSARPATLNLGYITPQQNAHYFLLNNPRRLMQAKGLDVQLSPPTAHHDSQHSVQAPTERAPFSGPIHDVSSPKGREEDSPNGPCEFVPVSPYQHLTSKPNEDSANGIQRQYYAPAERTDEGATSEQPHGNSADTPISHGPQNQGHESTDPSSLARPTKRRRQISTPNPLNPTSPQLPTAMRSPSTHSSPRPTPSASSLNPSRFPTPTSLNPEEPTDSPFQLPSHKQSRTTLFIAIPLSTDTVPLKLRSCMTLSSFLTAVLAICAQPGQHNHIISGIRVTFDWKQDKDVDRAILLKQDLPDTFEVLLEIVDGAPCWGEEGGRCGVAVEVVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJQ7</accession>
    <name>A0A8H6CJQ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009997</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153670.1">
        <property type="nucleotide sequence ID" value="XM_037300853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJQ7"/>
    <dbReference type="GeneID" id="59338389"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="139" mass="15378" checksum="C35B4BB3777F3DBC" modified="2022-01-19" version="1">MASTNYKEAFALFDKRGNGRVALESLGDLLRACGQNPTLAEIHDLEKGVGGDFDFDSFQKVLNRPGGFRDPGEPEEYCRGFQVFDKDMTGFIGVGQLRYILTNLGEKMSDDEVDELLKAVDTSSGEINYTDLVRTILAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKC7</accession>
    <name>A0A8H6CKC7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell division control protein 73 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC73 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153683.1">
        <property type="nucleotide sequence ID" value="XM_037300866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKC7"/>
    <dbReference type="GeneID" id="59338402"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11990:FF:000003">
        <property type="entry name" value="Pol II transcription elongation factor subunit Cdc73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11990">
        <property type="entry name" value="RNA polymerase II accessory factor, Cdc73 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007852">
        <property type="entry name" value="Cdc73/Parafibromin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031336">
        <property type="entry name" value="CDC73_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038103">
        <property type="entry name" value="CDC73_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466">
        <property type="entry name" value="CDC73 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466:SF8">
        <property type="entry name" value="PARAFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05179">
        <property type="entry name" value="CDC73_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Cell division control protein 73 C-terminal" evidence="6">
        <location>
            <begin position="244"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="423" mass="46416" checksum="92E50565EF741B8B" modified="2022-01-19" version="1">MANPPRDPLTSLRRSIASNNPPIPTTSPDAASSESVTHNLGVATHLHFTGPEPHSFSLDTPTRFISSDNPVDLRSIYFAWQQKDVNIPVYIASAQKLSDELGSTGRAGSKVQNLVFAERLDLITWLEGASDESEYIKGLESDSSAAKSAQVASGALGGVSTVPSGAAGARSGKQIDPRLQEIYNLERKMGDRNSILRGIKPTDFSHIRKHSEQFFKDRKKPAAAAPATATTTSLASRDKKPGRRPDPIILLSPSASSLLRMSNIKSFLESGTYIPPDSNLAGSSATANILHVSRLLPSIDNQRAFRFILVDTPEQFKPDYWNRVVAVFTTGQTWQFKSYKWQNAPDLFKNALGIYVGWRGEDVPGTVKGWGRGVTSAQVEKWNPQQGVSGRWRDREVVEGIWDRVEDSMRSRGWSKDGGPVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLY7</accession>
    <name>A0A8H6CLY7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154572.1">
        <property type="nucleotide sequence ID" value="XM_037300724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLY7"/>
    <dbReference type="GeneID" id="59338260"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="195" mass="20068" checksum="44D99CDA525664A5" modified="2022-01-19" version="1">MAAPVSTTLASLKLLPTKTEFTIYTDYCNSNATLVPLESCTSRSISTFSVDCPQLNGTSDTGYITSWGTSVPSTTSPSTSSVPVPPTSSSQQTLATDIPSTSFVPESQAPTSQPNPATGIPRPTQSSSTTFPPSPSSSQGQKGSGNDGGLSGPSTIAIGVVLPGVGVIVAIIIGIVGFKRHRRAPRALPTRRWST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMG1</accession>
    <name>A0A8H6CMG1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Structure-specific endonuclease subunit SLX4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SLX4</name>
        <name evidence="11" type="ORF">HO133_009083</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Regulatory subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a heterodimer with SLX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="9">Phosphorylated in response to DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SLX4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMG1"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033557">
        <property type="term" value="C:Slx1-Slx4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22999">
        <property type="entry name" value="SAP_SLX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03110">
        <property type="entry name" value="Endonuc_su_Slx4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027784">
        <property type="entry name" value="Slx4_ascomycetes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018574">
        <property type="entry name" value="Structure-sp_endonuc_su_Slx4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09494">
        <property type="entry name" value="Slx4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="113"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="641"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="141"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="700"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="734"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6226217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="847" mass="93105" checksum="30D4746292D50ED2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMG8</accession>
    <name>A0A8H6CMG8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154673.1">
        <property type="nucleotide sequence ID" value="XM_037299847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMG8"/>
    <dbReference type="GeneID" id="59337381"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="163" mass="18849" checksum="2842AA5930A35508" modified="2022-01-19" version="1">MLPNQTIHGIATRVYNFANRRKKQAYLYGSQTPPLMTKTEMTHKREQKEIQRCIYSSRYCRPVHILASTAADALATVLNDLDPRGFAETTSAISEFIHKAKTIREERKDNLITRREQLEKLRSLQVDLAIRFDRSHVRDGEVVLKRICDLATAAHKKRRRSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMH1</accession>
    <name>A0A8H6CMH1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009843</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154550.1">
        <property type="nucleotide sequence ID" value="XM_037300702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMH1"/>
    <dbReference type="GeneID" id="59338238"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36460">
        <property type="entry name" value="UPF0132 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G10255)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36460:SF1">
        <property type="entry name" value="UPF0132 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G10255)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="189" mass="21053" checksum="4BA52D2D9752A72B" modified="2022-01-19" version="1">MDFAPYQDTTPESTRTLSPPPNRISSPARNARSPPPTRPNTDPFGVSSLPEPSHFSDEPRENAGFGRGHIENGRLDVNLFETSLPLRLDYEAMLAYLLLPPAGGVFLLVVEHKSDYVRFHAWQSSLLFAAIFVLHLILSWSSILSWMLFVIDIGLIGFLAMHAYRDADGLDRFEVPFFGGLASSFVDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMU0</accession>
    <name>A0A8H6CMU0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Asl1-like glycosyl hydrolase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009166</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154853.1">
        <property type="nucleotide sequence ID" value="XM_037300027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMU0"/>
    <dbReference type="GeneID" id="59337561"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071966">
        <property type="term" value="P:fungal-type cell wall polysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053183">
        <property type="entry name" value="ASL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024655">
        <property type="entry name" value="Asl1_glyco_hydro_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154:SF3">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11790">
        <property type="entry name" value="Glyco_hydro_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034584651" description="Asl1-like glycosyl hydrolase catalytic domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Asl1-like glycosyl hydrolase catalytic" evidence="3">
        <location>
            <begin position="198"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="409" mass="43059" checksum="24A7609B181492E4" modified="2022-01-19" version="1">MMLSLVYASLLLAAGSNASPCKLHSGNWTSSSTTVAKAALHRDGHGSAAVNQNQKLGTQANAGLNLPLPYSQESPAPSSTSANNAGPSVAGSSDTSGTSDTSASLTPASGSCPQGFLNTVFNTNAGQSSGFPTATWNTLTEYGINNWSALRLQLQKTFLLTDKPFHLVGFSLDTLDKQADYDLSATQASQLSGAFNKAQIPIVMDHTYIQDAVGMLANNPPLYLELYNEPDFSFEGLTLTEEPAPSASDLKTLLDTPHPNTKFISPALMDANGPWLGEFFQACNGCIDDFHAIALHVYNPDPQGVLDQITQLYGTWNKTIWITELSPATAGCDLNTATMATYISTLIPEILKLGYVEKIFWNSGESDSTTINQAPAACSPSLTNSDGSFTPALQALGAACGINPGTATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP95</accession>
    <name>A0A8H6CP95_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155371.1">
        <property type="nucleotide sequence ID" value="XM_037299370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP95"/>
    <dbReference type="GeneID" id="59336900"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="205" mass="22795" checksum="558A1477AD601218" modified="2022-01-19" version="1">MHVQTCLPQQDLYRPSTSPSLVLSAFWIIVPSDPLNAAKRRLRTVVHSELSRSSEQVAHGTLQHQLNASKGWLRTVVHSELYRSNEQFAQGMLLLQRADSLVLQDRESELVSSARHRAFGAEKPVGESRSVSDNVEDKWYTSTGHLQSDNDIQAALSSKNVRPMPKSLTASNIADETVILLVRMLPDLHQLLEPTKALEPSGPCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPE7</accession>
    <name>A0A8H6CPE7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155448.1">
        <property type="nucleotide sequence ID" value="XM_037299447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPE7"/>
    <dbReference type="GeneID" id="59336977"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11062">
        <property type="entry name" value="CYP58-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF152">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="57811" checksum="102F436BD7113ECF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPX5</accession>
    <name>A0A8H6CPX5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008884</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155748.1">
        <property type="nucleotide sequence ID" value="XM_037299747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPX5"/>
    <dbReference type="GeneID" id="59337279"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056693">
        <property type="entry name" value="DUF7791"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF5">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25053">
        <property type="entry name" value="DUF7791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="2">
        <location>
            <begin position="257"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7791" evidence="3">
        <location>
            <begin position="535"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="951" mass="109326" checksum="55174208A21BB708" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPX6</accession>
    <name>A0A8H6CPX6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155686.1">
        <property type="nucleotide sequence ID" value="XM_037299685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPX6"/>
    <dbReference type="GeneID" id="59337217"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034412257" evidence="1">
        <location>
            <begin position="25"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="354" mass="37684" checksum="45A82111D6D0D7E9" modified="2022-01-19" version="1">MGINSTPSLAVLLSLLFKTLCVSAVEKSICSLSYFGLPDYHACYSLLFSGTKGRRRGINVIDGADHAFLLQDFAEASDFTKEQWKYKVYIPHVFKNEGCKILLAVQADPKGGFTHDSSHWPYIASDGRQILEDCLSPLHSGRPRSGGQEKSGSGRKLVMYLYSWGSSFDRDITLQSAAGNTIVASSDGAVGNVSVTAPFSKFFPIFPDKTPTPTNVTDFQYGQECSSNGDCRADGYVCVVSSIGESKSKLWAGTCELPYAEVSNGQKMNTSELTSAVSSNSSNTIELLEMVIPAACSCNCTYVSKSCCGSASGVVNEAAALKLGTLQAPSGQLCNPTTGRFQTVSASSDLSATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQY7</accession>
    <name>A0A8H6CQY7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007772</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155978.1">
        <property type="nucleotide sequence ID" value="XM_037298642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQY7"/>
    <dbReference type="GeneID" id="59336169"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000354">
        <property type="entry name" value="AGC/YANK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000469">
        <property type="entry name" value="Serine/threonine-protein kinase 32B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF30">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 32B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="47"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="412"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="467"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="434"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="576" mass="64145" checksum="52E64BFA1F69ADFF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRA0</accession>
    <name>A0A8H6CRA0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007846</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156052.1">
        <property type="nucleotide sequence ID" value="XM_037298716.1"/>
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    <dbReference type="GeneID" id="59336243"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF18">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE RASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="430"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="531"/>
            <end position="548"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="554"/>
            <end position="573"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="585"/>
            <end position="606"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="626"/>
            <end position="647"/>
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        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="662" mass="75087" checksum="EC68C6BDF84EF4BA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRA8</accession>
    <name>A0A8H6CRA8_9LECA</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">HO133_007782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="203"/>
        </location>
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            <begin position="228"/>
            <end position="291"/>
        </location>
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        <location>
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        </location>
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        <location>
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        </location>
    </feature>
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            <begin position="1"/>
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        </location>
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        </location>
    </feature>
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            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
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            <begin position="73"/>
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        <location>
            <begin position="96"/>
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        </location>
    </feature>
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            <end position="123"/>
        </location>
    </feature>
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            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1126" mass="125476" checksum="F1680BB511BEB9C1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRV6</accession>
    <name>A0A8H6CRV6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156454.1">
        <property type="nucleotide sequence ID" value="XM_037299118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRV6"/>
    <dbReference type="GeneID" id="59336647"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="132" mass="15173" checksum="67D105A3FD45508C" modified="2022-01-19" version="1">MGKAKNELEEFIANIPDSKLTGFPSIETTVYKTRNFRLDMQTVTTKDPRCYNLHVQINRGTLITSLKRFCREVALHRLGAGQRRGLDACGKRMYLKGRTPRDSTVIMKVAYREGHELQVPENYIKSVKTEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS11</accession>
    <name>A0A8H6CS11_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome c oxidase assembly protein COX15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_007911</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156115.1">
        <property type="nucleotide sequence ID" value="XM_037298779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS11"/>
    <dbReference type="GeneID" id="59336308"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120547">
        <property type="term" value="F:heme A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6228181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="44243" checksum="42F28401FC6E8461" modified="2022-01-19" version="1">MAGKVLIVQSLSITEWKPVTGSLPPATEEDWESEFNKYRSSPEFKMLNPNMTLSEFKSIYYMEWTHRLWGRFVGLSFAIPALYFIARRKVSGPMALRITGIAGLIGFQGVLGWWMVKSGLKDDLFATGSHPRVSQYRLVAHLGTAFLCYCAMFWNGLSILRTHKLLGNLQVAEASLRRLRNPRLRIFRLSVGALAVLVFITAMSGGLVAGLDAGLIYNEFPYMGLGLTPPKSELFSDFYSHTPDNSDIWWRNPLENPSLVQLDHRILATTTFTSVMALWAYSRFSSNVRTILPGPARKGVYGVVGFVFLQVVLGISTLIYLVPLPLASAHQAGSLALLSYVLILGSRSWLPNRRVSTLLAKRFATAKMTPKRSVQSKLRMRKQNNASEGAWKTAMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSJ2</accession>
    <name>A0A8H6CSJ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006950</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156481.1">
        <property type="nucleotide sequence ID" value="XM_037297846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSJ2"/>
    <dbReference type="GeneID" id="59335350"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="23"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="327" mass="37386" checksum="C7F479B7EE9F3BCE" modified="2022-01-19" version="1">MAQIFLDGLPQVNFETLPSESRCMICLEVYGTNLEDDGAVEVVVRLPCGHHVGAQCIRIWLSPNEEARNSCPACRRAFFPAQPRPYMEHGTIEQEEDDLEDEEDDWHGPSGRTNHPRRANLGVFFLDVLRMVGEAGQPPRPGQEVPQMEAMEEYTQDRVRGWWPEFFQTTTEQYQESIRRARAVITTPRVPPPGASVSHWSPYPYLQLSGSESINPEEGDPQHLDKVVQALATAFRTLSFRETIVYSILRDSGARDRFPSSSEDLLQPLSAEQEEALFREMERRGALGMDFGREYSGLNNSQRWQVHREDGETWNPYSGLWSPDWSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSN8</accession>
    <name>A0A8H6CSN8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_008211</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156415.1">
        <property type="nucleotide sequence ID" value="XM_037299079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSN8"/>
    <dbReference type="GeneID" id="59336608"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17974">
        <property type="entry name" value="DEXHc_DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000594">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF83">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="353"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="542"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="968"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="90"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="968"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6228481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="991" mass="112689" checksum="3D58270CDD50680A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSQ3</accession>
    <name>A0A8H6CSQ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006938</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156469.1">
        <property type="nucleotide sequence ID" value="XM_037297834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSQ3"/>
    <dbReference type="GeneID" id="59335338"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF134">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="27"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="229" mass="25752" checksum="3759144765CD9B5F" modified="2022-01-19" version="1">MAILETPGKMYALAAVLSVLAIVAAILRFYARRLLKSVRIEMDDYMIVLALVCHIFTLGTGLCMFIGAAFGDLGRHTRIDAQGMPIFTRRTQIFQQIAYASQLTQTLTFGFTKLAVLFFYRRIFTNGRVLYAIRTMFVLVTVWTVAFFFTNLLQCFPISVNWTGWGAAVDSCINTNEMFLAQAWSDVITDGNRPTAARARQNSNKLNSNDSLLTYSMRTLIPVLPSSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7U4</accession>
    <name>A0A8H6F7U4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147855.1">
        <property type="nucleotide sequence ID" value="XM_037296674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7U4"/>
    <dbReference type="GeneID" id="59334174"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="333" mass="38390" checksum="EED8854089DEED66" modified="2022-01-19" version="1">MPLITSLVSYESNLPPTAPLTSKCRTKQPIMPGLASTRFNDPCRYWDLWHHPEDLDSIIACLMWRDKHRLRAMDIAAALAFVQPAEYWGMSGEDVIKVYCYLQRHQNIPENPFADWQSMRDMRTHPHWRYYVIWASSCNVRRMQNLLDESLLPMHKQPDMSSYQQPPLNVSKDKPTDTEAFSQPVASTLSPKGCLPDTPYTLLLSEPPPSAFTMPSIHSIRPEDDVCGKWPGLWSQGHLMDSLICCMMFRDLHHMDEDSIAYVLGISYRGCAGMTGRDVVELYGFLQANPTLHSNPIKYWLDILNRNVEKPDGRRLVTLAILRFGRRSRPRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F814</accession>
    <name>A0A8H6F814_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147583.1">
        <property type="nucleotide sequence ID" value="XM_037297009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F814"/>
    <dbReference type="GeneID" id="59334511"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051704">
        <property type="entry name" value="FAD_aromatic-hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865:SF2">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="41212" checksum="F76DE771EC5B2CA8" modified="2022-01-19" version="1">MSKLRMLVSGAGIAGPTLAFWLARAGALVTIIERAPVLRAIGQNIDNCGAGIGVIQRMGLEDIICKNKTNEEGIAFVDAINRPKAQFEVYHSGKGKSFTSDTEISRETLAKILHDGTKEEADSTPQREFDLLVAGDGLGSPTRSLIFAHVGKICIRSLNQCTSYFSIPYQDPDGTLARWYNAPGRRCTLTRPNNAGKTIVFLSIISISEKLQSHSKLSQNEQKQSMHEIFPNAGWEASRMLAGMDTTDDFYMQSNAQVKLDSWSKGRMSVVGAAAFCPSPISGMGTTVAIVGAYILAGEISRCGTDYEKAFEAYESIMRPFVDTAQSLPPGAPAIANPETAWGNNLMNRLLDFVSWNGLESLLGRFGGPPADAIVLPGHEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F857</accession>
    <name>A0A8H6F857_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147718.1">
        <property type="nucleotide sequence ID" value="XM_037296537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F857"/>
    <dbReference type="GeneID" id="59334034"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034426593" evidence="1">
        <location>
            <begin position="17"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="180" mass="19227" checksum="7610BB06508FAB0D" modified="2022-01-19" version="1">MLFPLQTLTLISAISAAVLQPLQSINNQLLTTNGSEIFLFSNATNATASLPAEPGAAIARCSASNYGSNLNVKSCQNAFESVQSDDHYSSFGLRHHVAHYNEVLSFVWLSGGDGRLAVVMKPNSNANIQCEEAPIYPAPAGAVREWLRSCDDTLVDVSVTTTVKTFGPHEHPSVAVGLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8L2</accession>
    <name>A0A8H6F8L2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005306</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148198.1">
        <property type="nucleotide sequence ID" value="XM_037296216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8L2"/>
    <dbReference type="GeneID" id="59333712"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF11">
        <property type="entry name" value="AMP-DEPENDENT SYNTHETASE_LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="139"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="556" mass="60104" checksum="336AC86AAB583635" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8S8</accession>
    <name>A0A8H6F8S8_9LECA</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">HO133_005221</name>
    </gene>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex. Involved in pre-tRNA splicing, probably by affecting the interaction of pre-tRNA with splicing endonuclease.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148830.1">
        <property type="nucleotide sequence ID" value="XM_037296132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8S8"/>
    <dbReference type="GeneID" id="59333627"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042565">
        <property type="term" value="C:RNA nuclear export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Exportin-5_C"/>
    </dbReference>
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        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF3">
        <property type="entry name" value="EXPORTIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
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        <location>
            <begin position="115"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-5 C-terminal" evidence="4">
        <location>
            <begin position="337"/>
            <end position="1186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1230" mass="139097" checksum="D64CDC1E7D7F5BA8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8U1</accession>
    <name>A0A8H6F8U1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148982.1">
        <property type="nucleotide sequence ID" value="XM_037294935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8U1"/>
    <dbReference type="GeneID" id="59332425"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="674"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="861" mass="93816" checksum="F8F79F60E78B2D13" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F918</accession>
    <name>A0A8H6F918_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003612</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149222.1">
        <property type="nucleotide sequence ID" value="XM_037294534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F918"/>
    <dbReference type="GeneID" id="59332023"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="62574" checksum="D1563818513F7408" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9I1</accession>
    <name>A0A8H6F9I1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003399</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149702.1">
        <property type="nucleotide sequence ID" value="XM_037294321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9I1"/>
    <dbReference type="GeneID" id="59331810"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037830">
        <property type="entry name" value="ZZZ3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22705">
        <property type="entry name" value="ZINC FINGER, ZZ DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22705:SF0">
        <property type="entry name" value="ZZ-TYPE ZINC FINGER-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="253"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="307" mass="32187" checksum="1624F3566602C37F" modified="2022-01-19" version="1">MPPLSIPPRTPDPLHNDQQPDPRPPSPTAPPYSPITPTMSNSLPAPSTEAAGSSYTKPRIQHPPAPPPPAPLPSHAPAPQSSTTQPAPPPPPTPQMPALPPPVPVRECSNPDAIALRAAMSVLQLQRQQALRDMRTLERQKEAAVADPEAFARAVAGGKIRARGMRGILGPVGEVREGFDGADTTRAGGVDGGEGGGEVHGLGDGDDDDGATVGMGGGAAFGEIPSGQNVVRMPPVNWAKYHIVGASLDKLHEEQRSRPVAGQPLRDGDVKPRPRAPEHVVASPYDPWRDRLDGKDKGRTRSGRGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9P4</accession>
    <name>A0A8H6F9P4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytokinesis inhibitor byr4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037294620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9P4"/>
    <dbReference type="GeneID" id="59332109"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990334">
        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034586">
        <property type="entry name" value="Bfa1/Byr4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140:SF1">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="713"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="913"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="928"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1033" mass="111876" checksum="D1B8125A56EF906D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9S2</accession>
    <name>A0A8H6F9S2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003714</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149324.1">
        <property type="nucleotide sequence ID" value="XM_037294636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9S2"/>
    <dbReference type="GeneID" id="59332125"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="135" mass="14827" checksum="E148662CFB10D8EA" modified="2022-01-19" version="1">MPVHSALYAILPFISSKAAPAKADAVSLIAVTRKEPSPTTPSAFDADQFVAFDNALDSGEARCLVSNQLLCISKRHWSLHEQDEMNSIKSSAEQSARIMQEGHTDAWSVEEIGEDEEGHDESAERLQYAILRKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAC3</accession>
    <name>A0A8H6FAC3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002906</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149909.1">
        <property type="nucleotide sequence ID" value="XM_037293831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAC3"/>
    <dbReference type="GeneID" id="59331318"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15761">
        <property type="entry name" value="FYVE1_Vac1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15737">
        <property type="entry name" value="FYVE2_Vac1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.20">
        <property type="entry name" value="Rabenosyn, Rab binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052727">
        <property type="entry name" value="Rab4/Rab5_effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006642">
        <property type="entry name" value="Rad18_UBZ4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021565">
        <property type="entry name" value="Rbsn_Rab-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036531">
        <property type="entry name" value="Rbsn_Rab-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014010">
        <property type="entry name" value="REJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510">
        <property type="entry name" value="FYVE-FINGER-CONTAINING RAB5 EFFECTOR PROTEIN RABENOSYN-5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510:SF44">
        <property type="entry name" value="RABENOSYN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11464">
        <property type="entry name" value="Rbsn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00734">
        <property type="entry name" value="ZnF_Rad18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140125">
        <property type="entry name" value="Rabenosyn-5 Rab-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51111">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="REJ" evidence="10">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="9">
        <location>
            <begin position="328"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="269"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="566"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6220474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="647" mass="71854" checksum="A9DB0170F8E6A158" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAF8</accession>
    <name>A0A8H6FAF8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150118.1">
        <property type="nucleotide sequence ID" value="XM_037294040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAF8"/>
    <dbReference type="GeneID" id="59331528"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="521"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
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        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="592" mass="64993" checksum="1B6611B613C84807" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAG0</accession>
    <name>A0A8H6FAG0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Choline transport protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="ORF">HO133_002127</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150707.1">
        <property type="nucleotide sequence ID" value="XM_037293054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAG0"/>
    <dbReference type="GeneID" id="59330541"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF1">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="213"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="36969" checksum="4237CFE702C37D30" modified="2022-01-19" version="1">MTRGLITLTDPSYDPHPWRNVVLFWGVILFGVSPSHVSRTFINGENWPNDGLSFFVGLLGNVYAFFGADGAIRMSEEIQNAAAVVPKAIVFSIVLNGLLGFGIALALLFCIGDIDAALHTNTGYPFIEIFNQAVQNTTGAALMTSIVTTLSLCATVGIVASASRQLWAFPRDRAVPGREHLQRVNSNPAIPALAVFSTTAPACVLALIVLGSSTASNDIVSLSVVGLFGSYSLVAVLLLWRRLRGDIELYPSSGDGLTNLPGKEPTWGPWRVPGVLGIMTNSFAIVYLIIVFFFSLWPPVNRPTAATMNYGSLMFGGTMVFSVLYDLFRARNVHTGPVIQVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAG4</accession>
    <name>A0A8H6FAG4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7703 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003027</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150029.1">
        <property type="nucleotide sequence ID" value="XM_037293951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAG4"/>
    <dbReference type="GeneID" id="59331439"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056120">
        <property type="entry name" value="DUF7703"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37013:SF6">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37013">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_1G05950)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24802">
        <property type="entry name" value="DUF7703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7703" evidence="3">
        <location>
            <begin position="13"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="504" mass="56336" checksum="BEA914AA7D74216F" modified="2022-01-19" version="1">MAPPEGESGGPIPLNKGVYMAIAAFTAVAWYNVAELNVQVFLTFKRHRGLYFWSLLISSYGCVLHALGFLLKFFQLVSRNQSLNDYVAVTIITVGWYCMVTGQAVVLYSRLHLVVREQKILRAILAMIIVDAVCFHIPTTILTYGSNTMDSAKYIPAFNVMEKLQMTAFCSQEFIISGVYVWSTIRLLKPVYHGRTRKVMTQLIWINLIIIGMDVCLLAMEYSNIYEIEATLKAMVYSIKLKLEFAVLNQLMTLANSTVSSHQQLNIEEGSEKPTKPPTGRSRSTEYISNKYKKPSKKPKDNAKRSAPAIIHSSYSTSRVPHHSRSNSFGPNNKSRFQWIPTLDRNCVVETKHIENTEDCQLNVFTNPAACFKPGRQQPDGYPPHDGLGGIPNSCPDSPETSPTRDIDGSTLHSRRAPPPPHLDLDNSVMPNGLLRSTRPSVSEWGKGPDNESEASPNSDEIRLDPYENADYLPPRSPGSVRVGRDRAARSMGQLDFMTSALQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAP2</accession>
    <name>A0A8H6FAP2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002368</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150947.1">
        <property type="nucleotide sequence ID" value="XM_037293294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAP2"/>
    <dbReference type="GeneID" id="59330781"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034695178" evidence="3">
        <location>
            <begin position="18"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="390" mass="39957" checksum="4176BE9057FF1E69" modified="2022-01-19" version="1">MLPQIIVIATLAATAFSASTSGGGALAALPACAVCGNVNVTVSFSIGCPSETTTNSRGSLTTILTNRLGATVNDQGAFPTNYNNGSVEAGPSVATTGPVATFTGKPLLTGTCTIPQIATTTLNAGDILEYPWVGCSEEAPECCPFNINIGGKLIYTSALAGETPCYTTSSPAVQSPNATLSASGASVSMINTEVFTLRYALRQRQQSALSVGAKVGIAVGAACGGALALIALAIFIRKRRAQKRAQREEANMPKPESGNMYQGGFNGSQAAVSELPSPHTIRPPTPPLPDNAIWFNTPLGSHAPGRVMTPTELAGSTYINEHHPAYGVSPVTPNVNGFHDVPGPPEELAGDDLMRGTVSRETARTELVTRGEGAGSSPLSQESERAGGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAR4</accession>
    <name>A0A8H6FAR4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002664</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150418.1">
        <property type="nucleotide sequence ID" value="XM_037293589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAR4"/>
    <dbReference type="GeneID" id="59331076"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="178" mass="19858" checksum="E0DDFECACF962639" modified="2022-01-19" version="1">MSGYHSKVAIQDGQFAITGGFAMRVYGSTRQTFDVDMCVKPEPGYAPLLRAMLQSPRITLPSSKFIGAVLRCYVETGPSIGDNTQTDRAVKMDIMINGFLLPRFTHHTMKLTTNAQKICVLDRQCLLKNGIREYKHRLDQEDVEYFIDEGLVPTESQATVQQTKRILMLTGPPSPSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB72</accession>
    <name>A0A8H6FB72_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Steroid 5-alpha reductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150824.1">
        <property type="nucleotide sequence ID" value="XM_037293171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB72"/>
    <dbReference type="GeneID" id="59330658"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010721">
        <property type="entry name" value="UstE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251:SF17">
        <property type="entry name" value="STEROID 5-ALPHA REDUCTASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06966">
        <property type="entry name" value="DUF1295"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="35183" checksum="549B3DC167304908" modified="2022-01-19" version="1">MAVLSTLLHATTFSRPFLSTIVPSIALAYTLQAAVAVPSIIAQSERFYDLSGSLTYLSCTALSLYLPTIRARYAAAAAGAIKPAWPSVLGALRGASGAGLNWRQLVLSAAVSVWATRLGTYLFQRISADGSDSRFDDIRQSPPKFLGAFFAQATWVSLCLMPILAINSLPVSVFASLPAAVGITDVIGILLYVGGLSFEVVADRQKNGWVQEKKEKKHEEDFLTRGLWSKSRHPNYFGEATLWTGIATVAGGVLMSGPGQVGMGLAGWGLLGKAIAAGVCGVSPAFVSFLLLKVSGVPMSEGKYDKRYGDREDYKKWKRETPMLIPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBC1</accession>
    <name>A0A8H6FBC1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151545.1">
        <property type="nucleotide sequence ID" value="XM_037292126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBC1"/>
    <dbReference type="GeneID" id="59329612"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="166" mass="19239" checksum="5678C1D4FFBF541C" modified="2022-01-19" version="1">MASCVIPKAANEPPVFKEHSRNAVSHRTHYETARRTTTRISQPRTPRPVMTESPVYKPRMDLSNNLLFRLSLYYIFVSPETWQVASLLNQLHKIAPGIATQEVKSTYLHLHDTEHPMWVRAKDMAIQEPDELKRIMRDEIGLGGERWDVRVVKPVMAMAILRLIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBS4</accession>
    <name>A0A8H6FBS4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Condensation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000654</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151953.1">
        <property type="nucleotide sequence ID" value="XM_037291591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBS4"/>
    <dbReference type="GeneID" id="59329072"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043041">
        <property type="term" value="P:amino acid activation for nonribosomal peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45527:SF1">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45527">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="52213" checksum="0788D372EBC1412E" modified="2022-01-19" version="1">MDAGSPTTTNSRKEDTDKMIRACAEACGVGRQIIEKICPASEEQEHYMEHHISTGSSYMMQMVLHYEGDLNQNFLLRVLSAMRFKNHVLRTRLVKHEGHVYQVVLRDPIVFQQTGVDLHGFLAQNSRTRMDYGTPLSRYAFVREPHGEAFFVWTDLLAYSKLPPRPPYGKYAIWLASCVYEVGLTFWLTRHTTFDNLVHKFPVLGPGGTPSASTMKTKKLLHLPKVENPRFNLPTMGHAAWALTIAKFLTREYPPLVNEDISFISMSSARESDLPGIKRTMGPISARVPLLIRFLPDLSIENLMRDIETQLSSMIGFEHCAMKVLSREAGFQNMLKQAVFSWNPPDCDLSCKRIICYDKEAAPAVLDYREDLSVPFAHDYGLMFEVYEHGEHISIHATWDQNLVSGDLISRLLEDFGSFLALIIKTRGATVVELLSVNRVCQSGRTATFDPHFHLNTLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC54</accession>
    <name>A0A8H6FC54_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151829.1">
        <property type="nucleotide sequence ID" value="XM_037292410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC54"/>
    <dbReference type="GeneID" id="59329896"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="109" mass="11380" checksum="F164E38937D3B889" modified="2022-01-19" version="1">MYLTTGKFDPGGLIAGGGRQGHAFEGSEDESEPGVRANGLEALERLAEAKVANDVQSDVSVPVNLGSCALKAQGKKARFITLRIRAWSATLAVVTMMCGWPRGGGGEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCB8</accession>
    <name>A0A8H6FCB8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rRNA-processing protein EFG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">rRNA-processing protein efg1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_000740</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in rRNA processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EFG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152039.1">
        <property type="nucleotide sequence ID" value="XM_037291677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCB8"/>
    <dbReference type="GeneID" id="59329158"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019310">
        <property type="entry name" value="Efg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050786">
        <property type="entry name" value="EFG1_rRNA-proc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911:SF1">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10153">
        <property type="entry name" value="Efg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="264"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6222693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="312" mass="35423" checksum="E8EBA29390F40147" modified="2022-01-19" version="1">MQPHDVLGLVDRQEVNYEVVVFLFSVKYVTASPTKNSNLLQQIPFTWTQIIITSTDMGIKRPLHDDSMPSESASPRTPNHPSKRRKSNDFAIPAIKSASVNQLKSKIRDLTRALEHSDHLPAGVRIEKERALAGYKQDVEKIHEDKRKNELIKKYHMVRFFERQKATRAHKRLTKRLDASSPDAAEHKQLEREVHDAEIDVNYTIYYPLTEKYQSLFPRREDAGGVETAAKKLVTEKPAIWKVVERCTADGTLEALRDGKLATGVPVGNTKLALEKPRPEREGKKGRAESKSSKSAPLQEQDEESDGGFFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCF9</accession>
    <name>A0A8H6FCF9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable aspartate--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_001063</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152357.1">
        <property type="nucleotide sequence ID" value="XM_037291995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCF9"/>
    <dbReference type="GeneID" id="59329481"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04320">
        <property type="entry name" value="AspRS_cyto_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000038">
        <property type="entry name" value="Aspartate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="236"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6223011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="550" mass="61467" checksum="B3C8F5E4D453C4F8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCR8</accession>
    <name>A0A8H6FCR8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PH domain-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000545</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DCP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152919.1">
        <property type="nucleotide sequence ID" value="XM_037291484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCR8"/>
    <dbReference type="GeneID" id="59328964"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13182">
        <property type="entry name" value="EVH1-like_Dcp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF0">
        <property type="entry name" value="DECAPPING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="25939" checksum="ABF9ABE024DF54EF" modified="2022-01-19" version="1">MATTIYASHHPQPSDNELEYTAPANPTITNDVLNLSVLQRHLPSTVSIDFLAPYSVVYIFSTTTQAWEKSGIEGTLFVVKLRDEGHAVVVLNRRGLDNFILHLKSAEEVDVTDEYIILQGNSADNLGHGNADEQKVYGLWIFEEEQGSTKGVREACGRCIVERAERAGNEWGTRAVDGQSKGYAQQQSNVGAPNGPDLMALLNLSRGQGSNNMPAVQQPGSNQDQGVLGNLFRSAGANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDX5</accession>
    <name>A0A8H6FDX5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitobiosyldiphosphodolichol beta-mannosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.142</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_010875</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.142" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153358.1">
        <property type="nucleotide sequence ID" value="XM_037301724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDX5"/>
    <dbReference type="GeneID" id="59339265"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004578">
        <property type="term" value="F:chitobiosyldiphosphodolichol beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000162">
        <property type="entry name" value="Beta-1,4-mannosyltransferase (Alg1), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026051">
        <property type="entry name" value="ALG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036">
        <property type="entry name" value="BETA1,4 MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036:SF0">
        <property type="entry name" value="CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6224298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="469" mass="52505" checksum="770E75F371647BBB" modified="2022-01-19" version="1">MFDLESWLLGLVIILAISSLVLFNLPTQYGGIIGKDRSANRIQIIVLGDIGRSPRMQYHALSIAKHGAQVDLIGYRVSDIHPDILANSNAIRVHALHPTPACLQTKDSRLFLVYGPLKVLFQIWTLWLALGYRTKPSRWMLVQNPPSIPTLIVAYVVCFLRHTRLVIDWHNFGYSILALRLGSTHPLVQISRLYEQLFARSAEAHFCVTNAMARVLERDYGITTKILPLHDRPASHLQVLTEAQRSSFLATLPNLLSPDDDQRLQDYQRLVSEIRMSKSRLLVSSTSWTPDEDFSVLLEALTNYSDLAITTHPHLPEVLVVVTGNGPQKDAFLTRIQSLKAEEKLEMVTIETAFLSTSDYAELLGSADLGVSLHTSSSGVDLPMKVVDMFGTGLPVVGWGDFEAWPELLHEGVNGKSFSDAEGLQEVLMQLFGGDEELLRRLKKGAVEEGKLRWDEEWDRVAGTLFFPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDX7</accession>
    <name>A0A8H6FDX7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153677.1">
        <property type="nucleotide sequence ID" value="XM_037300860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDX7"/>
    <dbReference type="GeneID" id="59338396"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="64"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="150" mass="16002" checksum="882C345D8588FA89" modified="2022-01-19" version="1">MAEPKPTALTLAKDFVEALLAGSHDHGFDYELCKAMTVVSASLDPNPRAVTRLTVTLAMCNLSGTLHGGAISTLFDVCTTVALATARREGFWEMAGVTRTLSTTCLLPVFPGEEIEIVGEVVQIGKKLAHIIAKMTLVRNAAIFGRTSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEC6</accession>
    <name>A0A8H6FEC6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009958</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153631.1">
        <property type="nucleotide sequence ID" value="XM_037300814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEC6"/>
    <dbReference type="GeneID" id="59338350"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034721615" evidence="2">
        <location>
            <begin position="23"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="287" mass="28980" checksum="0D4AA363A34CCD11" modified="2022-01-19" version="1">MYLSFSFTTVFLLLASSIGITGLTSDLVGADSRSSGRNAAAIDARSIDGHRLGSKRSHDVSSRSSNHIAALIPREPQEGVPPPLPPVVKAPKPATAPESPTEPTAPEAPQAPAQKQEPEHKDDDDDDDDDDDDDHDGPEKPKEGSESGEGGDGGGDTDTGDSPEDGGNSTSSTSKCANPRGTASASCVSTATPLLGTMASAVPTPAMISSTSSSPTATMTTSATQEIAPASTAPAPTATSPTAAADGIGGRNPFAWVRGKSRGGGGIATTVGVYSVAYVACLVFFWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFB2</accession>
    <name>A0A8H6FFB2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Methylcrotonoyl-CoA carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_009756</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154463.1">
        <property type="nucleotide sequence ID" value="XM_037300615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFB2"/>
    <dbReference type="GeneID" id="59338151"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000028">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF33">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE SUBUNIT ALPHA, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0092">Biotin</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="12">
        <location>
            <begin position="42"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="158"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="10">
        <location>
            <begin position="638"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6225754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="720" mass="79125" checksum="1DCD8CEBAF317326" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFV3</accession>
    <name>A0A8H6FFV3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="9">HMG-CoA reductase</shortName>
            <ecNumber evidence="9">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009189</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154876.1">
        <property type="nucleotide sequence ID" value="XM_037300050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFV3"/>
    <dbReference type="GeneID" id="59337584"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3270.10:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.420:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.770.10:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="entry name" value="HMGR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025583">
        <property type="entry name" value="HMG-CoA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13323">
        <property type="entry name" value="HPIH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01192">
        <property type="entry name" value="HMG_COA_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0521">NADP</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="11">
        <location>
            <begin position="233"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1168"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1168"/>
            <end position="1184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6226323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1221" mass="130095" checksum="44D18FE7C88A0D6B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG42</accession>
    <name>A0A8H6FG42_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pescadillo homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Nucleolar protein 7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NOP7</name>
        <name evidence="7" type="ORF">HO133_008292</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pescadillo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG42"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17709">
        <property type="entry name" value="BRCT_pescadillo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03028">
        <property type="entry name" value="Pescadillo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010613">
        <property type="entry name" value="PES"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221">
        <property type="entry name" value="PESCADILLO - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221:SF6">
        <property type="entry name" value="PESCADILLO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06732">
        <property type="entry name" value="Pescadillo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BRCT" evidence="6">
        <location>
            <begin position="358"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="482"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="674"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="507"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="542"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="684"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="694" mass="77319" checksum="CA62CBA2FCBB577E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGZ8</accession>
    <name>A0A8H6FGZ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dopey N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_007947</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156151.1">
        <property type="nucleotide sequence ID" value="XM_037298815.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056458">
        <property type="entry name" value="TPR_DOP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF24">
        <property type="entry name" value="PROTEIN DOPEY-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24597">
        <property type="entry name" value="TPR_DOP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="8">
        <location>
            <begin position="26"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like middle TPR" evidence="9">
        <location>
            <begin position="390"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="10">
        <location>
            <begin position="1400"/>
            <end position="1868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1083"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="358"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1096"/>
            <end position="1105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6228217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1893" mass="210222" checksum="0C0ACE32BBE44658" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI47</accession>
    <name>A0A8H6FI47_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156593.1">
        <property type="nucleotide sequence ID" value="XM_037297958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI47"/>
    <dbReference type="GeneID" id="59335463"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="777" mass="85232" checksum="471EAB1C84A645CF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI60</accession>
    <name>A0A8H6FI60_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zinc finger ZPR1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007080</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156609.1">
        <property type="nucleotide sequence ID" value="XM_037297974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI60"/>
    <dbReference type="GeneID" id="59335480"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000003">
        <property type="entry name" value="Zinc finger protein zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="entry name" value="ZPR1, zinc finger domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="Zn_ribbon_ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="7">
        <location>
            <begin position="55"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="7">
        <location>
            <begin position="281"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="206"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="484"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="484"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="507"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="519" mass="57737" checksum="B8664ED1B4DC78B7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK52</accession>
    <name>A0A8H6FK52_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Golgi apparatus membrane protein TVP23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004321</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Golgi membrane protein involved in vesicular trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Golgi apparatus membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157240.1">
        <property type="nucleotide sequence ID" value="XM_037295239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK52"/>
    <dbReference type="GeneID" id="59332729"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6229983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="199" mass="22353" checksum="EB3E6FC37B0ADFB2" modified="2022-01-19" version="1">MENQAQQSKQGDLHWRLSSHPITLLFFLGFRMSSLLIYLFGLWFFTSSFVLVFIVTLLLLAADFYYLKNIAGRRLVGLRWWNEVDTTSGDSHWVFESQDRSGDSGGPVQNATDKRFFWLALYAQPGLWVGLAIFALVRLESPIWLSLNAIALILTITNTLAFSRCDKFSQASTLASSAMYSGGIAKTLAGGMFSRLFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK67</accession>
    <name>A0A8H6FK67_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase I-specific transcription initiation factor RRN3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRN3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157316.1">
        <property type="nucleotide sequence ID" value="XM_037295315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK67"/>
    <dbReference type="GeneID" id="59332806"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007991">
        <property type="entry name" value="RNA_pol_I_trans_ini_fac_RRN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790:SF0">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IA RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05327">
        <property type="entry name" value="RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="290"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="625"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="628"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="649"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="661" mass="74647" checksum="BF11EED7BF695D51" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKB6</accession>
    <name>A0A8H6FKB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAR-17a/AIG1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004429</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157347.1">
        <property type="nucleotide sequence ID" value="XM_037295346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKB6"/>
    <dbReference type="GeneID" id="59332837"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242:SF1">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242">
        <property type="entry name" value="OS02G0130600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="31505" checksum="7F411AA1226C6A1A" modified="2022-01-19" version="1">MAKIYPLLGIPQPFDPTCRFTTSWLLPPFLLASLRLLLSVYAFTTIFTVFGWDDAHHLAAAARQSFSYFTNLTYWGLAFYLLFGALHTFSYARTGQAWLRSWPRPLQAAHAVFYSTVVTFPFLVTLVFWAILYKDPWFPVVEEGWSNISQHALNSLFALVEVVLPRTSPPPPLHIVFLVILLACYLALAYITHATEGFYPYSFLDPSKGNGRVTGYCFGILIAICVIFGIVWGVIWLRNLATQKLGLEGKFAGQRRKADGLDVRERNVEVVELQEEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKJ6</accession>
    <name>A0A8H6FKJ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Mediator complex subunit 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED11</name>
        <name evidence="5" type="ORF">HO133_004571</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Mediator complex subunit 11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKJ6"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019404">
        <property type="entry name" value="Mediator_Med11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22890">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10280">
        <property type="entry name" value="Med11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6230231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="194" mass="20799" checksum="4D7ECCB447076D40" modified="2022-01-19" version="1">MSPDQPPIFSASDRIKHLNNIDKDVAKLLQSAGLAVKALTKPMAIAPAESLSSQPVPIEQQRESFTAATSQYFTLLSLVDVNLRRQIYALEEAGVLPAEAVTKEPPTSMAVPLAAQANPPNASSSRTAGGNKGVITGGGLGNLDVGWLNSRNDNVGKEMKAELWEEAERFVSKLEERKSGADRELDFLKGDGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZF7</accession>
    <name>A0A8H6KZF7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157115.1">
        <property type="nucleotide sequence ID" value="XM_037295114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZF7"/>
    <dbReference type="GeneID" id="59332604"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF2">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="235" mass="26936" checksum="D4DBE69945304211" modified="2022-01-19" version="1">MAPSTSPKTVTPAKRKFCEVSTVTLLVGKSKKPFHVHVNLLCDASPFFKAALAGKSKESSEKHMQLPEDDESTFELFVDWLYCQRYEMLPDDGDHEDDGGDNTDERFLQPFRLFVFADKYNVCKLKNLVIETLFVDGRGPLRGPGHRAIAYAYNHTTQGSGLRKLLADWYAWNIDLGWYEFQDIDAFLRQQPDFAPDVVLSFAKNLKWRHKQIHNPFAGDMPEEYKDKEGPGLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZH0</accession>
    <name>A0A8H6KZH0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein G</fullName>
            <shortName evidence="9">snRNP-G</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004243</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157163.1">
        <property type="nucleotide sequence ID" value="XM_037295162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZH0"/>
    <dbReference type="GeneID" id="59332652"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01719">
        <property type="entry name" value="Sm_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000019">
        <property type="entry name" value="Small nuclear ribonucleoprotein G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034098">
        <property type="entry name" value="Sm_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF2">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="3"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6229906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="83" mass="9212" checksum="19FC155F5461B728" modified="2022-01-19" version="1">MPQAQPELKKYLDKRLFVQLNGSRKVIGVLRGYDVFLNIVLDEAVEEKAGGEKVRIGMVVIRGNSVVMLEALERIGGGDRDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L004</accession>
    <name>A0A8H6L004_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004849</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157762.1">
        <property type="nucleotide sequence ID" value="XM_037295761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L004"/>
    <dbReference type="GeneID" id="59333255"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053178">
        <property type="entry name" value="Osmoadaptation_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="372" mass="41922" checksum="2D6FFF3013B770FF" modified="2022-01-19" version="1">MARLGWIHQDNTLVLNGRVLYGQALQEIQKALYDECTMWQDETLATGNILAFYEFLEPTNDSIAGWNSHTNGVTRLMLIRGPDRHRSSFGRALLEGIRLSAMIQALQNRKASPLGQYEWWQYPKRDLTQRLYDHGFALAALFEEIDKANLLGPEVETSLLTKYLRRCSALDATFNLWYHALTRQSSTPLYWLTPPNDGIHPSVASLTNRRPFSFPDLPAAHTVTTFWGCKLALSNTIAIVCASVLSPGRFNRHEDPRSFAALEQTARQLLIQHGDTGRLENATNIIRSMPYCLHDSMGLLGPQKSLFALRAALLSLRRTPGEELDWCLRMYQELAERKGLGYAREVGKVNAKDRVEAGKAMDAIRLAPGHGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L099</accession>
    <name>A0A8H6L099_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7924 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157819.1">
        <property type="nucleotide sequence ID" value="XM_037290945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L099"/>
    <dbReference type="GeneID" id="59328424"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057684">
        <property type="entry name" value="DUF7924"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42470">
        <property type="entry name" value="VAST DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42470:SF1">
        <property type="entry name" value="VAST DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25545">
        <property type="entry name" value="DUF7924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7924" evidence="2">
        <location>
            <begin position="198"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="414" mass="46421" checksum="5CE2024DB429E5C0" modified="2022-01-19" version="1">MSKSKQKVAELRSSTPVKSMPPPSTPYKPQSPYDRQAQSSQVELDGLEAATTATLASTIVSTESRKQTKNARSIRTSLEANGMWYNTTGRLESYPAFKNSIMATIDSQRGSSLRPESYKVIQEEMEENELSNEATYFHCLVEQVIKTGRSVDSGRKTANGTTHWNWKRFKEDGMSEPQKDQKMARGFLPGQLTDTANVDIGLTDPKPDWVFGITMTTKFAAGQAPSPEVRDLLELGFGLWHAFFVIEGKGNQQPITVARNQALRDGSALVNGRRAFNKLAHDTGVQDPLGPDYDSFCFSCTWDINIAELWVHWYELENTASPGVFHMHLLGRYVMIGRMTEVAKFRHDVHNILDWGILTNAAKRVETMAKIKAKETAQVSILNGRASPQKRQESPRKGRVPPTKRESSPRKRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5S1</accession>
    <name>A0A8H6C5S1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000029">
        <property type="protein sequence ID" value="KAF6217418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146853.1">
        <property type="nucleotide sequence ID" value="XM_037297830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5S1"/>
    <dbReference type="GeneID" id="59335334"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="109" mass="12691" checksum="82008DE6E32A98DE" modified="2022-01-19" version="1">MTGDLKLDISSPVSRQYVVDKFNNGFFQKILMHYESEMGESLSENSQFRPEPGRIFPEKEMKREDENLQNFLTQHNAYMEAKQENAARENGSQDEGEIDNEIQNIQLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C602</accession>
    <name>A0A8H6C602_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147128.1">
        <property type="nucleotide sequence ID" value="XM_037297418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C602"/>
    <dbReference type="GeneID" id="59334921"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="228" mass="24485" checksum="E58707DD89A4178B" modified="2022-01-19" version="1">MRFVVSFHTGNPDKTYYLQPPALWSAGELASAILCGCMPATAAFVNHVSPKLARTWSLYLRPSPDGTPRAGGAPRAPLPRSSWRAPRAWNESYLLRSGSDGEGEVGMRQLLLHKPLILIPPIGPLVQLPERRNISPTSSPVAQNSVDVAPSATESVPGMRPLDAGSRQRCVTCDVDDADTVLGTARGGRGPEDREQCFGKQEGGKVIGLPLSFEAVGRELEGDCHNLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6C4</accession>
    <name>A0A8H6C6C4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147155.1">
        <property type="nucleotide sequence ID" value="XM_037297445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6C4"/>
    <dbReference type="GeneID" id="59334948"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="622" mass="71360" checksum="490DA9AB2BD1EC95" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6I1</accession>
    <name>A0A8H6C6I1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Clp R domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_006678</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147011.1">
        <property type="nucleotide sequence ID" value="XM_037297575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6I1"/>
    <dbReference type="GeneID" id="59335079"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000120">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000010">
        <property type="entry name" value="Chaperone clpB 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="entry name" value="Clp, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF18">
        <property type="entry name" value="HEAT SHOCK PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="Double Clp-N motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="10">
        <location>
            <begin position="3"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="894"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="435"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="902"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6217576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="935" mass="104280" checksum="0A87353D1536C006" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Y8</accession>
    <name>A0A8H6C6Y8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147548.1">
        <property type="nucleotide sequence ID" value="XM_037296974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Y8"/>
    <dbReference type="GeneID" id="59334476"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="254" mass="27962" checksum="61EFD66303C901D9" modified="2022-01-19" version="1">MSDELPPYTPQALTDILPIYHPANTNHHISIYTLHQTAPDTLVLTLRDHPSESATAYTSTLPVCKIRTNKTGGFMNRKPHVIITTGECDESLNRPLAEGRFDIHGTGTTISYSHLPNIQMIQRLELEDSLSQRLRTTIWGHEHWWTPNPGNKGVLELTNEMDEIVARFVHTVPVSQMTGSGKRGSTGRKKEAREELGDLHIVDALAEGGREEVICSALVVVERAKRRANNISCTGIGKTAPAWSMSAGPPGGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C729</accession>
    <name>A0A8H6C729_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006134</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP domain-containing ribonucleoprotein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147610.1">
        <property type="nucleotide sequence ID" value="XM_037297036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C729"/>
    <dbReference type="GeneID" id="59334538"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052240">
        <property type="entry name" value="SAP_domain_ribonucleoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551:SF1">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="3">
        <location>
            <begin position="94"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="319" mass="36284" checksum="66020735A816DD6E" modified="2022-01-19" version="1">MPFRHQPVTKPIRTKYDKLKVDILRGLLALRVLPTTGKKADLTMRLFQDDNNNERLAALRALWQQPAVVKPSSDPDPGQGPSLCYPRQNHTSYYESISIAQLVYHLHERALSVHGDQEDLVKRLRDYDWDGSDSGYSESDDEEEEDHTASFYHRTLTHQSLYDGKGLHPSILIGLRIQGFFYDAKKGLQLHCDRGCFNLTFCGWPWPTSVGLPLCHTDRIRLDKVLWNGLRSVQQLDEQGLNNSIGLDKGEKGENLLIVSAVIAVRKGMGGDYRVLGLQCEGMSTMGYVYADDREACRVKCPGRFVRFFDVAVDRRKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7U8</accession>
    <name>A0A8H6C7U8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005917</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148002.1">
        <property type="nucleotide sequence ID" value="XM_037296821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7U8"/>
    <dbReference type="GeneID" id="59334322"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF1">
        <property type="entry name" value="INHIBITOR OF NUCLEAR FACTOR KAPPA-B KINASE EPSILON SUBUNIT HOMOLOG 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="132"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="456" mass="48907" checksum="452C50D32BD41CA4" modified="2022-01-19" version="1">MPEDTPQDTASKQRWLPLTSPPTSPVALPMRPALAELPALPLNQRPQLTASKTLPAEVPVVGANQPLTPPSTPPKPENIRASASADGIEAILSINVNLGAKPFGMPELKADQASKRKALGPDTAPKPYTGEYQLREELGRGAWSTVYRAVENVEPLKQLTNLPPTPPTSPTAGATSSSKKVLAVKKPSSRQAHKILEKEAKILTYIHSHANASTHLVPFHGFDSAQHSIMFDAVPLSLETHAKAAIRRPLSTKTMFDPVVGAEEWAGLAESLIDGLAFLHSVGCVHGDIKPANILLRPGSSRALTPLYCDFSSSHTISSTGEEQEVEEVSAVTTEYLSPELLEALSPRNTDRAVATYASDVFALGVTLLFAAIGDSPYAGARMASQKLGMAKEGLPFEFARRGEQASRVMKGRAVERALKGALAREAVKRAAVAKWRDEIREPLKSWEEGGWARGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C863</accession>
    <name>A0A8H6C863_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148074.1">
        <property type="nucleotide sequence ID" value="XM_037296893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C863"/>
    <dbReference type="GeneID" id="59334395"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="221" mass="23165" checksum="AF0EC5D632F914F7" modified="2022-01-19" version="1">MSSTTTNNTCIIYGTGCASPTNLPSYIDDQACLCGLLESRSSLPNNGEHIELWRCIGNASDNVEDGGNGKWYNTSLPSQELSGINQPQNWGENPPDLSQAYVLQDENGQAVYEELGSAGSINLIGSDKDCTGINDTSMSEQYYARGNGVPKSSSTSTSLMPTAQSATKTSATESTMQTASPSAAAAPPKGDNKSSAIHQVSLKSTFLLGMVLAPLLFGLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8J6</accession>
    <name>A0A8H6C8J6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc finger CHCC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148390.1">
        <property type="nucleotide sequence ID" value="XM_037296408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8J6"/>
    <dbReference type="GeneID" id="59333905"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.40:FF:000003">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.40">
        <property type="entry name" value="q5lls5 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019401">
        <property type="entry name" value="Znf_CHCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13156:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13156">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-A SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10276">
        <property type="entry name" value="zf-CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger CHCC-type" evidence="2">
        <location>
            <begin position="138"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="215" mass="24403" checksum="CE3E1BC24C22545D" modified="2022-01-19" version="1">MFAPIRGAWISSIHPRLHPRLLPLRSYSTSDRTSDRVPTNDPSPPKPVQNVSDSNAVPISPQGLRDSDRPLQEMPEDSERQRVMQAPNRKDVWSRSQQPRERAMSGPRFEQTIMEYQPQPEAAISLIHKQPVRWTKTRTVSCDGGGGPLGHPRIFINVDKPQICWCSYCGLPFAHEHHRKLLQSLPSTTYPLEPVGHPAEVPESQRVNDEPFGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8X5</accession>
    <name>A0A8H6C8X5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148249.1">
        <property type="nucleotide sequence ID" value="XM_037296267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8X5"/>
    <dbReference type="GeneID" id="59333763"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="197" mass="22173" checksum="B731A30C0C0EC344" modified="2022-01-19" version="1">MERSSIGKTRASKLLNRRCQSGSNARAGVVEMRLAVEPDQNSVRSAPTPLSFSDPVIDKKVLIASSSLSSSTEWDSFHLHPYTHRLTLRLPILHVYSHTLLLPRTDLFPQIYYNRHLSHSEPHSRLILLLATPPEPESQQPPDPAHHNAAIDVILDSMKLSHNEFLDKMAQMTRAERALGGLRRIFAGFNQAIEEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Z9</accession>
    <name>A0A8H6C8Z9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005476</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148368.1">
        <property type="nucleotide sequence ID" value="XM_037296386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8Z9"/>
    <dbReference type="GeneID" id="59333882"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12323:SF0">
        <property type="entry name" value="CALCIUM HOMEOSTASIS ENDOPLASMIC RETICULUM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12323">
        <property type="entry name" value="SR-RELATED CTD ASSOCIATED FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="25"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="588" mass="64337" checksum="96F456BA6FC3C460" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C919</accession>
    <name>A0A8H6C919_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148324.1">
        <property type="nucleotide sequence ID" value="XM_037296342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C919"/>
    <dbReference type="GeneID" id="59333838"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="143" mass="16442" checksum="20D6647CF6B9B172" modified="2022-01-19" version="1">MVAQNDLGFLNRPVYYVKVKGSKRMEIKVVIHEQTGTRYGLPGCFRDLLACPVLRHVTVKVGLFRAGLYYDTDAEWSYMSEDRVLDKTNSDGLDEILTLTSGMYRQLKEKIGEELKFETNYLRVGEKIKAWEERIGKAAVEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C976</accession>
    <name>A0A8H6C976_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Rad50/SbcC-type AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_005123</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148733.1">
        <property type="nucleotide sequence ID" value="XM_037296035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C976"/>
    <dbReference type="GeneID" id="59333529"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="14">
        <location>
            <begin position="80"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="449"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="838"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="696"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="842"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6219298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1004" mass="113970" checksum="0CC14EFEC63214D8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA01</accession>
    <name>A0A8H6CA01_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA2/NAM7 helicase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148908.1">
        <property type="nucleotide sequence ID" value="XM_037294861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA01"/>
    <dbReference type="GeneID" id="59332350"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="173" mass="19599" checksum="09017FAC786B4C7A" modified="2022-01-19" version="1">MEVLKSTPRSSHFGIACPYSDQRRETRKALLKAGLQNIRVGTIEFWQGEEIDLMIMDLVHAGNDHGALDFLGKKERLKCSSYSTDPASIRLWSKEKHATRKIRCAPEDVEKEAESEASQAARATLKSNLQQQINQELKDQLRKELRGEMEASIRSEIQQEVKAAYQAKLASLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAG3</accession>
    <name>A0A8H6CAG3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Spermatogenesis-associated protein 20-like TRX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149255.1">
        <property type="nucleotide sequence ID" value="XM_037294567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAG3"/>
    <dbReference type="GeneID" id="59332056"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006402">
        <property type="entry name" value="UCP006402_thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 20-like TRX" evidence="1">
        <location>
            <begin position="17"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="737" mass="82266" checksum="74FAA271129A20C4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC28</accession>
    <name>A0A8H6CC28_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150263.1">
        <property type="nucleotide sequence ID" value="XM_037293434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC28"/>
    <dbReference type="GeneID" id="59330921"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034106805" evidence="1">
        <location>
            <begin position="20"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="79" mass="8805" checksum="C9299981ECF66F3D" modified="2022-01-19" version="1">MKATIYMWGCLALSLACNALPVSRREGLLPRAESVDGDDRVVYTWSLPDEKSKRAESADGDDRVVYTWSLPDEKSKVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCB8</accession>
    <name>A0A8H6CCB8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150079.1">
        <property type="nucleotide sequence ID" value="XM_037294001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCB8"/>
    <dbReference type="GeneID" id="59331489"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF3">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="285"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="871"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="922"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="971" mass="107190" checksum="86B379C0C541EBB2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCF9</accession>
    <name>A0A8H6CCF9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CP-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_002691</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150445.1">
        <property type="nucleotide sequence ID" value="XM_037293616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCF9"/>
    <dbReference type="GeneID" id="59331103"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04178">
        <property type="entry name" value="Nucleostemin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000006">
        <property type="entry name" value="Nuclear GTP-binding protein NUG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000844">
        <property type="entry name" value="Nuclear GTP-binding protein NUG1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CP-type G" evidence="9">
        <location>
            <begin position="185"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="73"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="540" mass="58695" checksum="02E6D3FA72D1BBA0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCU4</accession>
    <name>A0A8H6CCU4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150306.1">
        <property type="nucleotide sequence ID" value="XM_037293477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCU4"/>
    <dbReference type="GeneID" id="59330964"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="306" mass="34359" checksum="4D6F491A6031C704" modified="2022-01-19" version="1">MASSSKSVPSLPSYGSEGSSNDVFPPKEEQAALPEADEPALPTITAIRIPADGTLPCLITLQLVKATSNYHYSLYDSNLLRHSNADDMRDINRHSCKFFNPMVDDAQIDLDKLYDSRDSLYEKATWSLEELENHPGLHAHSYVKGTDKFLDVPLAEKTEEPHLTFTHASLRLQPNVVDPFWGSGEAWNHRAFQRLYAVPREYADLEMMTGEYHIVYTQTLGMGLHPNKWARDRVFGDAFVLKMASGKNDEGDWYYENMPQKILHCSLGNQCLETLRSVRPIAWKFMVGERGGGPPGVISPGTGLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD46</accession>
    <name>A0A8H6CD46_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150697.1">
        <property type="nucleotide sequence ID" value="XM_037293044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD46"/>
    <dbReference type="GeneID" id="59330531"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059009">
        <property type="entry name" value="Znf_C2H2_17_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059095">
        <property type="entry name" value="Znf_C2H2_17_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26177">
        <property type="entry name" value="zf_C2H2_17_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26176">
        <property type="entry name" value="zf_C2H2_17_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="217" mass="24482" checksum="458B6C0ADF943D4F" modified="2022-01-19" version="1">MLFSKHLDSHLKPYRCKSNECSDTHFSSTACLLRHEREAHGMHGHGAKPFLCKFEGCERAHENHGFPRRWNLLDHMKRVHGYSGSEPSNNSDSPSPSDSSSHNSHKDPTNGQRKRRTPSPTENVVAKRAKLATSRADGQASTCSASGSGSVAMAIREQTGPHRSQQYPPEELLLRDHQVRLDSRLRNRDFSDPQALKQYHGDNAMFENLARKVYGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD78</accession>
    <name>A0A8H6CD78_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 6, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Complex III subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_002316</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UQCRH/QCR6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150895.1">
        <property type="nucleotide sequence ID" value="XM_037293242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD78"/>
    <dbReference type="GeneID" id="59330729"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.20:FF:000003">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.20">
        <property type="entry name" value="Ubiquinol-cytochrome C reductase hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003422">
        <property type="entry name" value="Cyt_b-c1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023184">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.8 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02320">
        <property type="entry name" value="UCR_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome C reductase hinge" evidence="12">
        <location>
            <begin position="81"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="17"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="42"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6221460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="150" mass="16908" checksum="661B3F324FAEA9FD" modified="2022-01-19" version="1">MGFSDIFSDFYNSMGFTELHAEAPPEEEKEDDEGGDDKGEEKSDDGDKEEGEEEGGEEEGGDDEGGDDEEEEEEEEEEPEDPKPKLEEQCARSAQCAPAKHHYEACAERVTKQHENPNHKGPKEDCVEEFFHLQHCATQCAAPKLFRQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDB8</accession>
    <name>A0A8H6CDB8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Scaffold protein Nfu/NifU N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NifU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150774.1">
        <property type="nucleotide sequence ID" value="XM_037293121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDB8"/>
    <dbReference type="GeneID" id="59330608"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000001">
        <property type="entry name" value="NFU1 iron-sulfur cluster scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.70:FF:000001">
        <property type="entry name" value="NifU-like protein 4, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="2">
        <location>
            <begin position="76"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="317" mass="34495" checksum="197282961624494A" modified="2022-01-19" version="1">MSTIFPRASLQSLGVLRPSATASRAVPRCSKALHQYTTSNTLTASSIRLRPRLDSLNSRSKFQPHALAGTIRTIFIQTENTPNADALKFLPNHTILPEGLASPFLEYLSPRSTLAPPHPSPLAARLLNVDGITSIFYGPDFITVTKASDAIWAHVKPEVFSLITEAVTSGEQIVNTIENKSAEAGQEGGEQDTLGYDENDSEVVGMIKELLETRIRPAIQEDGGDIEFRGFENGHVLLKLRGACRTCDSSTVTLKNGIESMLMHYIEEVKAVTQVLDQEEEIAMQEFAKFEAKLKEQKGPDAIASTVGKGSLDTAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDC1</accession>
    <name>A0A8H6CDC1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vanadium chloroperoxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150589.1">
        <property type="nucleotide sequence ID" value="XM_037292936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDC1"/>
    <dbReference type="GeneID" id="59330422"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03398">
        <property type="entry name" value="PAP2_haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.606.10">
        <property type="entry name" value="Vanadium-containing Chloroperoxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016119">
        <property type="entry name" value="Br/Cl_peroxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052559">
        <property type="entry name" value="V-haloperoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041067">
        <property type="entry name" value="VCPO_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34599">
        <property type="entry name" value="PEROXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34599:SF1">
        <property type="entry name" value="PHOSPHATIDIC ACID PHOSPHATASE TYPE 2_HALOPEROXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17897">
        <property type="entry name" value="VCPO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vanadium chloroperoxidase N-terminal" evidence="2">
        <location>
            <begin position="33"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="591"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="600"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="613" mass="67477" checksum="CD299BDE5237AB75" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDI7</accession>
    <name>A0A8H6CDI7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150621.1">
        <property type="nucleotide sequence ID" value="XM_037292968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDI7"/>
    <dbReference type="GeneID" id="59330455"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="263" mass="28992" checksum="23677A1926D8C3A6" modified="2022-01-19" version="1">MNLTSSLLALASQNLSSTLQDIGGYSYCQEYYGLFPNSRDCVKAIALLEKGNTDVSYAVHSGIGPHALPLSRSYGTCMIQVDVAGPRLPRTYDIIPDEIRHLADEVLNSCVTGPFEIGGFATTDLQVMNGWITAEETELDRPFPTSTAFLTVTLAQVVPDWLSPGNYDPVMAFHFSQVALRAIDRAFTLRERIDLRERAERLLRQGERMQPRGNRVPWWVNPDSGEEANGTSLETGNGTVSIDLPEPLASVADTATSKQRRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE03</accession>
    <name>A0A8H6CE03_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001756</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151223.1">
        <property type="nucleotide sequence ID" value="XM_037292684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE03"/>
    <dbReference type="GeneID" id="59330170"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16514">
        <property type="entry name" value="RING-HC_LONFs_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF42">
        <property type="entry name" value="LON PEPTIDASE N-TERMINAL DOMAIN AND RING FINGER PROTEIN C14F5.10C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="199"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Lon N-terminal" evidence="7">
        <location>
            <begin position="279"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="512"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="520"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="64780" checksum="C49491993C971689" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE21</accession>
    <name>A0A8H6CE21_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001682</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151149.1">
        <property type="nucleotide sequence ID" value="XM_037292610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE21"/>
    <dbReference type="GeneID" id="59330096"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47251">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04180)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47251:SF1">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04180)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="42"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="168" mass="18907" checksum="E261D427C6EDD8DC" modified="2022-01-19" version="1">MPPPWSTNPAPKTESAREARKSFYCELCQKGYSRINEFEAHEGSYDHQHKKRLKEMKQMSRDPLAAAKARKAERRADEKSGGITIKPVSLPSAEATKPTGFKKGGFKNAFGSADDDEEETDEKKQTKAEPGVEGWKVAEPVEMESDEEWGDYDPRRPTGCWEGCPGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEB4</accession>
    <name>A0A8H6CEB4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151327.1">
        <property type="nucleotide sequence ID" value="XM_037292788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEB4"/>
    <dbReference type="GeneID" id="59330274"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="241" mass="27232" checksum="DEA744092038E695" modified="2022-01-19" version="1">MPRGTNKRKEAEEGSSDETPPVRQNKTRRTSGNTVKFAHTVQAVSFGEYQQPKSILKTRSEPFPIAPGSPSTARSPSPEPSKEFEEEEFDREEVDTMRKLAEAAINLARKARKEQLEHDLAAVQKELAFTKNDLENVRFSEKIYMQSTEKVMQSRDKLVRALADADKRTETARAEVKEALLAQRKSENKSRALELQLKRADEQVEKLQQELAVMREKEETGKLLGKKTAMEKADGGNGDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEU3</accession>
    <name>A0A8H6CEU3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GDP/GTP exchange factor Sec2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001169</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151518.1">
        <property type="nucleotide sequence ID" value="XM_037292099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEU3"/>
    <dbReference type="GeneID" id="59329585"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009449">
        <property type="entry name" value="Sec2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430:SF4">
        <property type="entry name" value="GDP_GTP EXCHANGE FACTOR SEC2 N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430">
        <property type="entry name" value="RABIN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06428">
        <property type="entry name" value="Sec2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144284">
        <property type="entry name" value="Sec2 N-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDP/GTP exchange factor Sec2 N-terminal" evidence="4">
        <location>
            <begin position="105"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="152"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="234" mass="25966" checksum="46C476BD0862E13B" modified="2022-01-19" version="1">MSSVTTTILQSSPAAPIPITSFTKTCHKCGADLSHSHPPIAEDAQRRIAELETQVKLLTGKATAAVDKLADYEDELRQLKSSNKPTSDPNTNAQPPSRPTSAAGAQRNHLQSRISHLLPSNRRATSQPPSSTPADQTSHTNNRENAELLTMLTREQSLRKAAESRVSQTNEELEELSAQLFTEANEMVATERKARAKLEERVAVLEGRDKEKRTRLEVLESRLGRIERVREMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFH3</accession>
    <name>A0A8H6CFH3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001327</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151676.1">
        <property type="nucleotide sequence ID" value="XM_037292257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFH3"/>
    <dbReference type="GeneID" id="59329743"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034232576" description="Peptidase A1 domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="59"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="553" mass="59173" checksum="6C49FE39DB6EF52A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGE8</accession>
    <name>A0A8H6CGE8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Solute carrier family 40 member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001104</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in iron transport and iron homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152398.1">
        <property type="nucleotide sequence ID" value="XM_037292036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGE8"/>
    <dbReference type="GeneID" id="59329522"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009716">
        <property type="entry name" value="Ferroportin-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660:SF57">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06963">
        <property type="entry name" value="FPN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="521" mass="56385" checksum="48B26C6C81C30AF8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGE9</accession>
    <name>A0A8H6CGE9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="ORF">HO133_000959</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Forms DNA polymerase zeta with REV7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="28">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152254.1">
        <property type="nucleotide sequence ID" value="XM_037291892.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59329377"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05778">
        <property type="entry name" value="DNA_polB_zeta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05534">
        <property type="entry name" value="POLBc_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000007">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.342.10:FF:000018">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000002">
        <property type="entry name" value="DNA polymerase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000024">
        <property type="entry name" value="DNA polymerase zeta catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056447">
        <property type="entry name" value="REV3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24065">
        <property type="entry name" value="REV3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 20" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 20" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13 20" id="KW-0408">Iron</keyword>
    <keyword evidence="14 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase zeta catalytic subunit N-terminal" evidence="27">
        <location>
            <begin position="4"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="26">
        <location>
            <begin position="61"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="24">
        <location>
            <begin position="876"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="23">
        <location>
            <begin position="1138"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="25">
        <location>
            <begin position="1633"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="523"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="635"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="1651"/>
            <end position="1678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="421"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="551"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="640"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="660"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF24065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="KAF6222908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1735" mass="197208" checksum="BB56640303FB9A8E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGJ4</accession>
    <name>A0A8H6CGJ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000887</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152182.1">
        <property type="nucleotide sequence ID" value="XM_037291820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGJ4"/>
    <dbReference type="GeneID" id="59329305"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050815">
        <property type="entry name" value="TF_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338:SF27">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0843">Virulence</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="215"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="58"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="294"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="843"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="959"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="844"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="959"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="975"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6222836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1051" mass="116842" checksum="BF1502620DE4FCB0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGV2</accession>
    <name>A0A8H6CGV2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF28 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000177</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TACO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152552.1">
        <property type="nucleotide sequence ID" value="XM_037291117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGV2"/>
    <dbReference type="GeneID" id="59328596"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048300">
        <property type="entry name" value="TACO1_YebC-like_2nd/3rd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049083">
        <property type="entry name" value="TACO1_YebC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532:SF0">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20772">
        <property type="entry name" value="TACO1_YebC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="YebC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="TACO1/YebC-like N-terminal" evidence="5">
        <location>
            <begin position="7"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="TACO1/YebC-like second and third" evidence="4">
        <location>
            <begin position="69"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="43766" checksum="AA1D7B6A16B084BE" modified="2022-01-19" version="1">MTGASVTKQRSTLTQDLQLASKQYGADPQFNPRLATVITAAKKQGFPKASIENAIARGQGRSPTGAALESLTIEAMLPPSVAVIVECQTDSKARTLGDMKLAIKESGGSMTPTSHMFDRKGKIVFEGSKDIGEEEIFDHAIEAGAIDVQMEDEGKVVVYAESSQTTAVAENLVNSSGLKVESLDIVWDPKEDMMVDVASPEMLDSFLGGCNHIRPFNTPGSPWSMKLVLLGLGLALTVQGARTEQKDSHQERELNTKDLVAEETDQIEEKSDDDGPEKEEDADFMDSAVDEEENGEDKNEQHDWKDIEEDEDDIQNCGKESEVSALAIIVPSQMVREIPRITIDKETGRIFNHMPFAERLRILAALAFAVTIWIFVIGFALWTMVQASENIIVTVVFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGW3</accession>
    <name>A0A8H6CGW3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 13">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="11 13">3.2.1.106</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Glucosidase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_000160</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="14">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="11 13"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152535.1">
        <property type="nucleotide sequence ID" value="XM_037291100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGW3"/>
    <dbReference type="GeneID" id="59328579"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000027">
        <property type="entry name" value="Probable mannosyl-oligosaccharide glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.110">
        <property type="entry name" value="Glycosyl hydrolase family 63, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031335">
        <property type="entry name" value="Glyco_hydro_63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031631">
        <property type="entry name" value="Glyco_hydro_63N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038518">
        <property type="entry name" value="Glyco_hydro_63N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028095">
        <property type="entry name" value="Mso1_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF11">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03200">
        <property type="entry name" value="Glyco_hydro_63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16923">
        <property type="entry name" value="Glyco_hydro_63N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14475">
        <property type="entry name" value="Mso1_Sec1_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Mso1 N-terminal" evidence="17">
        <location>
            <begin position="20"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 N-terminal" evidence="18">
        <location>
            <begin position="234"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="16">
        <location>
            <begin position="463"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="56"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF14475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6223318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="975" mass="109458" checksum="371C74FF2D910CFF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIL2</accession>
    <name>A0A8H6CIL2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153219.1">
        <property type="nucleotide sequence ID" value="XM_037301585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIL2"/>
    <dbReference type="GeneID" id="59339123"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="37104" checksum="802164D772E3DC1A" modified="2022-01-19" version="1">MQITIQMLDGSITPFKARSSITIASLKAKFEPWQPMKGRALLHHDVQLDDRAILGSVPGVKDGTILRVDEQVSQVKDKATGKKRLMSGPARSAVDEDVVESIEQSGGDDEETPSEDEKRISSSKRRRPLRTPAGTVEDFIDDGNIANSPQGKALVLDDMSDDTVRTADTNLSDRAKALHIAKKEKGKNTAREPTDMLASKAQNIGHRLHALNTFAPPPENPVQNPTASAHMPKTTEAISSRQDVMEDDDATFLAGINAAAAATQRLPLHCEAGPEEDVSVHRCDACGRACRCGGDRSATSNSVVPVEETNRGREAASTNSNLHAHLIARRNPASQHAESSWTGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIP7</accession>
    <name>A0A8H6CIP7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153368.1">
        <property type="nucleotide sequence ID" value="XM_037301734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIP7"/>
    <dbReference type="GeneID" id="59339275"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="23214" checksum="321AC32E83E41C83" modified="2022-01-19" version="1">MPDLKLPEATWGRPPGRTAKRKRPDPDFDIAIDPDSQVDRFKRQNIGNDTIDLTPSHRIPDVLGSQETDPAYTTSTRESQRSQLPAPLLDLLSSQPLRKPSISPSYQSTTLPSESQSPDLPASAISELETLSADNQLDKPNDKGKAIQKWDPKVWDENEFDSDEENMSLAVTMKLATELSLLKSFLEIYRKFNIHSQQWDCGLVGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ23</accession>
    <name>A0A8H6CJ23_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amidoligase enzyme-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_011097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153580.1">
        <property type="nucleotide sequence ID" value="XM_037301946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ23"/>
    <dbReference type="GeneID" id="59339487"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022025">
        <property type="entry name" value="Amidoligase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36847">
        <property type="entry name" value="AMIDOLIGASE ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36847:SF1">
        <property type="entry name" value="AMIDOLIGASE ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12224">
        <property type="entry name" value="Amidoligase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="45868" checksum="F8ED2D873DFAD040" modified="2022-01-19" version="1">MPKPLNLTFGIEFECIVSFDPTTYKPALHAAEGILWEKKVSSLLHEESKLCILFREHLVHLLRANGFPTYDVTSAGGNQKWTVSHDTSIDIKDGPRSQDGFLECDIEITSPAMRFCPKALRRVQHVIRTLTKHLDVSVNPSCGLHVHVGNQKAGFPLQTLKHFCILTALFEYQLNSLHPAHRIGNCHAKGPSAAFKGHDLWDTVRAIQGCETEEELVLLYADKEDRPDRCFAYNLCPMVFGSNKTIEFRQHEGTLEWSEIMNWVQVAGGMVDAMHDISSFGLAQLIGTCAFDPRFSVSDLLRRLRLKALVPYYSGHLHMHQRPEPVRVRGGIEGNVEGVPHGRHAVSGWNELERRHKFEKLEELERLRMLAKRHELERRRERARQNEAIDARRATSEYEGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ26</accession>
    <name>A0A8H6CJ26_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_011050</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153533.1">
        <property type="nucleotide sequence ID" value="XM_037301899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ26"/>
    <dbReference type="GeneID" id="59339440"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000163">
        <property type="entry name" value="3-phosphoinositide-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000128">
        <property type="entry name" value="Serine/threonine-protein kinase ksg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="306"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="158"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="608"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="689"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="826"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="612"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="642"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="689"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="874"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6224473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="892" mass="97076" checksum="F6EA4CB77198F3E8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ36</accession>
    <name>A0A8H6CJ36_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_011000</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153483.1">
        <property type="nucleotide sequence ID" value="XM_037301849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ36"/>
    <dbReference type="GeneID" id="59339390"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000010">
        <property type="entry name" value="26S proteasome regulatory subunit RPN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040780">
        <property type="entry name" value="Rpn6_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040773">
        <property type="entry name" value="Rpn6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18503">
        <property type="entry name" value="RPN6_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18055">
        <property type="entry name" value="RPN6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="224"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="424" mass="47668" checksum="CDFE1BD364A84E4F" modified="2022-01-19" version="1">MAPAPTDSARVEEAKKLGKTDPLKAEAIYKEILSKKPGASEAALREYELALMGLGESYRDTRKTDELAELVRSSTSSLSSFTKAKTAKLVRQLLDLFSSIPNTVDIQISVTKSCIEWAISERRSFLRQNLETRLVSLYMQKQSYYDALTLINGLLKELKRLDDKLVLVEVQLLESRVYHALGNQPKARAALTSARTSAASVYTPPLLQAGLDMQSGMLHAEDKDFNTAFSYFIEALEGYHSQEETQKATSALQYMLLCKIMLNLADDVNNLMTSKQAMKYSGKNLEAMKAVARAHSNRSLEDYEKALANYRYELGSDVFIRNHLKRLYDSMLEQNLIKVIEPFSRVEIDHIAKMVGLDTQQVERKLSQMILDRVIIGVLDQGAGCLIVYDEAERDQGYDAALETIEKLSNVVDTLYTNQASMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKA2</accession>
    <name>A0A8H6CKA2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lon protease homolog, mitochondrial</fullName>
            <ecNumber evidence="11">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PIM1</name>
        <name evidence="17" type="ORF">HO133_009980</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer or homoheptamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12 13">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKA2"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141164">
        <property type="term" value="P:mitochondrial protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070407">
        <property type="term" value="P:oxidation-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000113">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000001">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000003">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000015">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03120">
        <property type="entry name" value="lonm_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027503">
        <property type="entry name" value="Lonm_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="16">
        <location>
            <begin position="173"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="15">
        <location>
            <begin position="882"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="62"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="283"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="814"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="814"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="974"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="1017"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="594"/>
            <end position="601"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6224786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1096" mass="120992" checksum="9F4B72BC1BBA2681" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKA3</accession>
    <name>A0A8H6CKA3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153757.1">
        <property type="nucleotide sequence ID" value="XM_037300940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKA3"/>
    <dbReference type="GeneID" id="59338476"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="132" mass="15280" checksum="1F7A0457E40191F9" modified="2022-01-19" version="1">MEAINNRLKQLEDIETEDRQLQLKRQEEHRDFLEALRERDQEEDVSTLRSPTRRSRVKGSAQELRRRRRILSRSSFGHLAVGSRDDESQASTDVENWPVTRSETKVKRSASKIVPSSSSGRKDYGLPTARDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKC5</accession>
    <name>A0A8H6CKC5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutaminase</fullName>
            <ecNumber evidence="2">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010422</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154092.1">
        <property type="nucleotide sequence ID" value="XM_037301275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKC5"/>
    <dbReference type="GeneID" id="59338813"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="291" mass="32489" checksum="BA7DC9EF7D09F77C" modified="2022-01-19" version="1">MMSDKMALDVHNHQMTRNIVVGVLALQGAFYEHLKLLQRAFREVAIGQDEGWEFVEVRTAQELERCDALIIPGGESTTVSLVAARSNLLEPLRDFVKVRRRPTWGTCAGLILLAESANRTKKGGQELIGGLDVRVNRNYFGRQTESFEAELALPFLGTKDGRGDVKPFNGIFIRAPVVEKILPVIRGEQASERAIDETVIAPSRSPEGEMNEQVLRPVEVMATLPGRSRTLKKHVAATGLEDEAGDIIAVKQGNVFGTSFHPELTEDARIHVWWLQQVKDMTQHEEKSRFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKM6</accession>
    <name>A0A8H6CKM6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153937.1">
        <property type="nucleotide sequence ID" value="XM_037301120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKM6"/>
    <dbReference type="GeneID" id="59338657"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16620">
        <property type="entry name" value="vRING-HC-C4C4_RBBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928:SF3">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="733"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="777"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="854"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="872"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1003"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1013" mass="110664" checksum="8815F0A269469B49" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL33</accession>
    <name>A0A8H6CL33_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PEBP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010358</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154028.1">
        <property type="nucleotide sequence ID" value="XM_037301211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL33"/>
    <dbReference type="GeneID" id="59338749"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF82">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034884270" description="PEBP-like protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="27950" checksum="19F11A434A30EE74" modified="2022-01-19" version="1">MQLNPFATHSLLFTTLLQPSLAQTPSNFAFSTTNPLIVQFNTTTVSPQGELLPEAAVALPPTLGTTAVLPGTYLAVMVDPGAGTATEVFEVLHYLLPGLTSPDAPATRNGTAFYPLTTAAQPLAPYLVPAPTPGDAHSYTLTLWKQPEGFAVPGAFLRYLPLNASDPTNRYPFNLTGLVAAAGLGQPLAAGYFDVQNTTASATTTAGHTTTSGGASSTTSSSGHSTSEGSSTATGKTVAATSSAALIGAEGCVSVWSVGCFAWALLALAGVAARM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLQ4</accession>
    <name>A0A8H6CLQ4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154245.1">
        <property type="nucleotide sequence ID" value="XM_037300397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLQ4"/>
    <dbReference type="GeneID" id="59337931"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="448" mass="50681" checksum="A664FF17AACCE5DB" modified="2022-01-19" version="1">MNRPIGTMSGEAQRRSNPRNFLSLPPELRHVIYRHLLIPSPIVKMKRKAYDLEPAILLVNKKLYQEALWVLYRENDWILLHVKAPFQTHLDGWHLENDFQRYPIVPLAEKSFSQKPCLEVEICETGAQVRGRAGGDPRRFLVSLDGLTKVCQILTAAKNTSSLEIILSFVEVKADTLNQIMDCLEEVCGYGNVVAKGLDAEATQKAINKVMMAEASTGKETLERTTKYEAGITEHIAHGRYIPAMKACQDGMAYIDFVYNNLGNRDLNPHSSLVPTLLKFTNFALKHARCCLACEDFQSAREIMNLLLGSDPHTSLYQPSRPYVSEAHFYYGQSMVAGGAENAAAFSFFRALDVVPSHEGANAELDAMEVRLALRGPDAETQRVRQNLQTLGAWRHRNEQDGRLPHAERTKRISEFLLSDDERIPFEKRLKAIIDRNEPPFSKFVNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM77</accession>
    <name>A0A8H6CM77_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154768.1">
        <property type="nucleotide sequence ID" value="XM_037299942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM77"/>
    <dbReference type="GeneID" id="59337476"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF112">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="338" mass="36815" checksum="A353FC955009BB46" modified="2022-01-19" version="1">MAFVLRRPMSITTCIKQLPKPSQSTSRAFHSSPKPSTFTTKSFTPITALTKSRNAFRQSFRRTYMQPGGQVVRPDTGSMAQRLLYGAAIVGGTVVATNFMFNRETREDGGMPPYERAFLNETFMHTGLGIGIIGVAANALHRSGWSYRLMTANPWVVMGVGLAASVGTMYGTFATSPDNYVQKYALWGGFNLCQAAILSPLMFMQPAILARAGLYTVAMMGSIAFVGATAKQEKYLYLGGPLLAGVAVVAVSGLAPLVLPVTAARTLMLTEKIWLYGGLAVFGGFTLYDTQKILHHARLANAGMVKKDVVNESISLELDFLNIFIRMVQILGMRNQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMZ2</accession>
    <name>A0A8H6CMZ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Actin-crosslinking protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009338</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FRG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155025.1">
        <property type="nucleotide sequence ID" value="XM_037300199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMZ2"/>
    <dbReference type="GeneID" id="59337733"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23339">
        <property type="entry name" value="beta-trefoil_FSCN_fungal_FRG1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010414">
        <property type="entry name" value="FRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928">
        <property type="entry name" value="FRG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928:SF0">
        <property type="entry name" value="FSHD REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06229">
        <property type="entry name" value="FRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="29084" checksum="BDC949F46A57B09D" modified="2022-01-19" version="1">MVKPLAFKGDRKSKKRKNPHPDSCLTLEDGDTKALTVQNLAAEAEEDDSWVTAEAATDVTGPIMFALPSTKPTCIGCDANGKVYTSELENIVHGDLATAEPHDVRQVWIASRVAGTDEISFKGHHGRIGVLSATREAISPEESFLCIPSPETPGMFSVQTAREKFITIVDESKVPEIRGDAESVSFNTTLRIRMQARFKPRLKANKESKTREKISRKELEEVVGRRLEEDEVRRLKKARVQGDYHEAILDVKVKGKHDKYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP68</accession>
    <name>A0A8H6CP68_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008601</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155467.1">
        <property type="nucleotide sequence ID" value="XM_037299466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP68"/>
    <dbReference type="GeneID" id="59336996"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034286650" evidence="2">
        <location>
            <begin position="25"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="359" mass="39896" checksum="B2CCCABA35022F45" modified="2022-01-19" version="1">MPFLSYSHFLWIFVLVAFAVTSNADVTPQYNEEFAARALVAPGITNVTNLNNRFADLSLDYISIRSEGFTIDQVALGFINAVKREAIKDCDQYIASYTFTDTEYADDPIRIQINAAAGGPEGQLVRCNLVYAIKTLAIDLMTQEILYGARFTESYRGQLLYVGVFDNKNDVPSLEQSSNSWADPSETLAQERRALSTQSLNATNSSTTLLAMTGSNDVEYRIEFDFRKNRVPKTAIFSAILEFMMTLAQLDNDDQIENVSQATSTDSSWIFVMHNSESSVSLEGFQLVAILEGIARHAVHWGRYVEMTFDFFVNREFVAGGCVTVPNRSRAWCQGLREGGPQSLVKNSSSSSGSDLIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPA1</accession>
    <name>A0A8H6CPA1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor G, mitochondrial</fullName>
            <shortName evidence="10">EF-Gmt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Elongation factor G 1, mitochondrial</fullName>
            <shortName evidence="10">mEF-G 1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Elongation factor G1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MEF1</name>
        <name evidence="12" type="ORF">HO133_008370</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPA1"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04097">
        <property type="entry name" value="mtEFG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04091">
        <property type="entry name" value="mtEFG1_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000003">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000001">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000022">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000015">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000558">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047872">
        <property type="entry name" value="EFG_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636:SF2">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
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        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="32"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="116"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="170"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6226929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="733" mass="81666" checksum="2955EA0DB2A50F4C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPE9</accession>
    <name>A0A8H6CPE9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155446.1">
        <property type="nucleotide sequence ID" value="XM_037299445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPE9"/>
    <dbReference type="GeneID" id="59336975"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="112" mass="12109" checksum="D5D1C344D8EEDA1A" modified="2022-01-19" version="1">MLMSSTTSVAALFIAPHVENEEFEHVVELACGHILCKKDDDKATGARCGLLGSNSLFSSLFSNLSIYEATRCPICESNLLANRNVGSEHEFGGVPKKHTTVAKSGELWGPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPQ9</accession>
    <name>A0A8H6CPQ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Beta-lactamase-like ARB-00930-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="59336818"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058664">
        <property type="entry name" value="ARB_00930-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26335">
        <property type="entry name" value="ARB_00930_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-like ARB-00930-like C-terminal" evidence="1">
        <location>
            <begin position="154"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="305" mass="32914" checksum="353BCF40B15D3EFD" modified="2022-01-19" version="1">MASVNTTSGPQLKSNQIQQTVSRAVDLVQCPHQSTASDPPAAATLEVPPIYDSSNLIRVPAQYLRVPTALPEPAALFSEAIFSLPLRPDDPNNRVTDLNTKEGNIGLYRSSLILSPDHNIGITVLVASTESGPERTMLKNSLIDTFLPAVEHEAQQQASNRFSRVYISPGNSSKTSFAIATDDRPGLGLFNATTINGTPLQLIAESQGLGGANKTLSVRLYPTGLKTTATTVDKKGIYTSRMSFRATFEALPQSSGMGWCQTWESIDGNTYGGVGTEEFVFGFSERGSVAYVEWRALRERLIKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ11</accession>
    <name>A0A8H6CQ11_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155439.1">
        <property type="nucleotide sequence ID" value="XM_037299438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ11"/>
    <dbReference type="GeneID" id="59336968"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="119" mass="12532" checksum="FA45C4A9ECDA37AE" modified="2022-01-19" version="1">MDNQQSQSFKPKTDFTMDNPKSQNPQADEQSSAGGRGGLGDKLSSVAGEGTGGSGDSQSLLDKGISYLQQSVTGQSGQTDEASAQGDTSHHAAVDEAHPEKISEFLRDQNRSMTKEEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQS4</accession>
    <name>A0A8H6CQS4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155839.1">
        <property type="nucleotide sequence ID" value="XM_037298503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQS4"/>
    <dbReference type="GeneID" id="59336030"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="691" mass="78399" checksum="51FAF2A1C8920010" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRN2</accession>
    <name>A0A8H6CRN2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Terpene synthase</fullName>
            <ecNumber evidence="4">4.2.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007979</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the terpene synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.-" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156183.1">
        <property type="nucleotide sequence ID" value="XM_037298847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRN2"/>
    <dbReference type="GeneID" id="59336376"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034686">
        <property type="entry name" value="Terpene_cyclase-like_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35201:SF4">
        <property type="entry name" value="BETA-PINACENE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35201">
        <property type="entry name" value="TERPENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19086">
        <property type="entry name" value="Terpene_syn_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="340" mass="39371" checksum="F16FD76B637AFCA8" modified="2022-01-19" version="1">MTQEQPISILTALHGQSIILPDLNAILDGWPREVNQNLDRLRHDVDEWLGSAMSHSPKLETLKAADFGYFGATWWPRATYERLRIVTFLAAWLFVWDDVVVFPEIDMDNGSLWDNFELSNPYRNETREYVQYCLSLTSEPDIGPVKTVNATIQAFETIGTAIRKVYNFEQRKRFMNYMEDFLEMSQQEQMLRLRGTLPSLEEFWLYRPGSSAVHVTIALNEFAWDNKSLPTSVMEDGDMKLLWDYTNVIISTVNDIVSLKKEIARGSISSMIPLLYARSRDAQVAVDQTAEFLVVNVKAFEETAQRLLKQEKRHNVEEARELRAFIKGCQFYCSGNLTWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7B2</accession>
    <name>A0A8H6F7B2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">endo-1,3(4)-beta-glucanase</fullName>
            <ecNumber evidence="3">3.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_006369</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;3)- or (1-&gt;4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.</text>
            <dbReference type="EC" id="3.2.1.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.6" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147392.1">
        <property type="nucleotide sequence ID" value="XM_037297267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7B2"/>
    <dbReference type="GeneID" id="59334770"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02181">
        <property type="entry name" value="GH16_fungal_Lam16A_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000114">
        <property type="entry name" value="Probable endo-1,3(4)-beta-glucanase NFIA_089530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF24">
        <property type="entry name" value="GLYCOSIDASE C21B10.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26113">
        <property type="entry name" value="GH16_XgeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034149606" description="endo-1,3(4)-beta-glucanase" evidence="7">
        <location>
            <begin position="19"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="8">
        <location>
            <begin position="29"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="341"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6217957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="371" mass="39571" checksum="635894C9EC521373" modified="2022-01-19" version="1">MYCSTFLVQAILLELCAAGYVLETSYTSANWFDQFSFFTSGDPTAGYVNYVDQSTATNNGYINTSNGLVYMGVDSTNVATGSGRDSVRITSNAAYNYGLFALDASHMPGGICGTWPAFWMVGPNWPNDGEIDIIEGVNDNANNAMTLHTSDGCSITDGAFTGTLTTSNCYVYAPNQSNNAGCDIQNQNTQSYGNNFNSNGGGVIVMEWTSSDINIWFFPRGTTPSDLEAGTPDPASWKEPVAQYQGDCDIPSHFADMQIVFNTDFCGDWAGAVWGTSSYCSSLADTCNDYVQNNPSAFANAFWLINSLDVYQSDGDVVAVSHVAVSPGGHQRNETAPLLSAPLAMGGGLPNPPGKRAMRRGRSTMGGNGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7H1</accession>
    <name>A0A8H6F7H1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein ERB1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic ribosome biogenesis protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ERB1</name>
        <name evidence="10" type="ORF">HO133_006633</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat BOP1/ERB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7H1"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000061">
        <property type="entry name" value="Ribosome biogenesis protein BOP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03027">
        <property type="entry name" value="BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012953">
        <property type="entry name" value="BOP1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08145">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01035">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
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        <property type="entry name" value="WD40 repeat-like"/>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BOP1 N-terminal" evidence="9">
        <location>
            <begin position="159"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="426"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="748"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="312"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6217806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="773" mass="86719" checksum="E1742960F06F0FC1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7P2</accession>
    <name>A0A8H6F7P2_9LECA</name>
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        <recommendedName>
            <fullName evidence="16">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="16">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_005679</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="16">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Associates in the nucleolus with the 60S and pre-60S ribosomal subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="16"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147767.1">
        <property type="nucleotide sequence ID" value="XM_037296586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7P2"/>
    <dbReference type="GeneID" id="59334084"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="DEADc_DDX18"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000460">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DDX18/Has1_DEADc"/>
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        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="159"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="190"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="380"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="607"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="159"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="611"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6218332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="637" mass="70437" checksum="AD68C334E0430667" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7R4</accession>
    <name>A0A8H6F7R4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147541.1">
        <property type="nucleotide sequence ID" value="XM_037296967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7R4"/>
    <dbReference type="GeneID" id="59334469"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="62" mass="6928" checksum="7C7A6C48D513255B" modified="2022-01-19" version="1">MDKVTETGLEVELPTGVQITPQPDENKYYMRGGESDHPGFVQSDQSNQSHADPEPFGWHQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7W1</accession>
    <name>A0A8H6F7W1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SRR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147900.1">
        <property type="nucleotide sequence ID" value="XM_037296719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7W1"/>
    <dbReference type="GeneID" id="59334219"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080">
        <property type="entry name" value="SRR1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080:SF1">
        <property type="entry name" value="SRR1-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRR1-like" evidence="2">
        <location>
            <begin position="98"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="327" mass="36509" checksum="FC7C89E9EA0A0634" modified="2022-01-19" version="1">MASLESQPDSHADRSESPSASARPSPSLDGSTSITEGMRPLSLARFHTSRPAAVWEPAPGTSIDTLLAKHERFTEAWKASEAYRELHEFFTDVLLKINTFQIKSCMCLCLGSITAGRPCLEDFDVPCTNSLSQLVAFESWVELLKSKHPIEQMYMQDPGFNDLDIEFLELRGFKVIESPASDDCMTPDTFLFTPGGEQDPVVAAIDAAHPALYIGNDLRDHPYITVNPSIFLGGYSPTFQEQLMNSLPAAEPLVRQALLPFMNKRILKPMPTSDLATWLEGEIMYYKPASDSELVPVAFNKSDEELYDDEQAEAYWREQRSHGFATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7W5</accession>
    <name>A0A8H6F7W5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Asl1-like glycosyl hydrolase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147691.1">
        <property type="nucleotide sequence ID" value="XM_037297117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7W5"/>
    <dbReference type="GeneID" id="59334619"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071966">
        <property type="term" value="P:fungal-type cell wall polysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053183">
        <property type="entry name" value="ASL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024655">
        <property type="entry name" value="Asl1_glyco_hydro_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154:SF3">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11790">
        <property type="entry name" value="Glyco_hydro_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034654278" description="Asl1-like glycosyl hydrolase catalytic domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Asl1-like glycosyl hydrolase catalytic" evidence="2">
        <location>
            <begin position="46"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="309" mass="33094" checksum="28910ECF98467DE2" modified="2022-01-19" version="1">MLFTSQSIFFAISALFSTLQAQPHGHIHHHGTSKRATGVVSGRGVVYADGNSGMAALGGKVSWSTDWTPWEDNPTNADLGNFAPQAWGLNNADGDPQKQFLTAFLNASSTFPEGATVLGFNEPEQVTQSWMTPQAAVDNWGGINDLKAQGHKICTPCPSNGNNNGTSGTGTVTLQYQSGIDQGTAWMSEFLAAAKGQYTFDCICGHWYGGQDNDLAADQSMIASQMGEMAALASEYGIEEIVIAEMQRVNGDQECEFVAWFQDTFLTDPQYAESGNKISAYSYNYDKFTLTSGDSLTTVGSAYIGEGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8R4</accession>
    <name>A0A8H6F8R4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005175</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148785.1">
        <property type="nucleotide sequence ID" value="XM_037296087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8R4"/>
    <dbReference type="GeneID" id="59333581"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF14">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034739758" evidence="5">
        <location>
            <begin position="20"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="60"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="66"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="110"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="407" mass="42017" checksum="99FFB2A450BF04FF" modified="2022-01-19" version="1">MRAGLLIIGCAALLRLSTAQLHDVVTDVVTDIEYVTYDSEDVIVYVNDANQPVSTTTVYKGLVPHTPAPTPSNPPAPPAESPSNTPAPPPESPSTPPAPPSKSPSSPLVAPKPKPSTPAPAPSPVSQPTEAMAPAPSPNPSQNPSPEESGSGGPGFSSAVAYTPYNADQSCKSASQVAADFQKISGYEVVRLYGTDCNQISNVIAATHSSVKLLLGIFDINSLQSEVQIISSAVNGDWSLVNSISVGNELVNTGQASASQVVAAIGTAKAALKAAGYNGPIATVDTMMAMKNNIELCTASDFCAINCHAFFDGNTLPEDAGQFVQNWVKQISEAAGGKTTIVTESGWPHQGDPNNKAIPSPENQAAAIASLKAAFGGGSNLVMYNSYNDLWKSDSADTFGSVFLAMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9B9</accession>
    <name>A0A8H6F9B9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149522.1">
        <property type="nucleotide sequence ID" value="XM_037294141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9B9"/>
    <dbReference type="GeneID" id="59331629"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="127" mass="13188" checksum="193C2988B5E7052F" modified="2022-01-19" version="1">MAEDNEELNIRQPEQTDQNEEATPDPSAPPEKKGSMFSAPGNFGNSAGDKIQGTLGKVGGPVGKGLETVAAPVGGLIDPLVGGLFRAPETLKNATQESEKVDQHNEELEKPIAGQEQTGGNPLGLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9D3</accession>
    <name>A0A8H6F9D3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fungal N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003920</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148889.1">
        <property type="nucleotide sequence ID" value="XM_037294842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9D3"/>
    <dbReference type="GeneID" id="59332331"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="474"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="752"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="779" mass="87459" checksum="9204CEBEB5DBC194" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9F7</accession>
    <name>A0A8H6F9F7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6604 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149341.1">
        <property type="nucleotide sequence ID" value="XM_037294653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9F7"/>
    <dbReference type="GeneID" id="59332142"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046539">
        <property type="entry name" value="DUF6604"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38795">
        <property type="entry name" value="DUF6604 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38795:SF1">
        <property type="entry name" value="DUF6604 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20253">
        <property type="entry name" value="DUF6604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6604" evidence="2">
        <location>
            <begin position="37"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="530" mass="60092" checksum="72B0F31FED075E30" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9I6</accession>
    <name>A0A8H6F9I6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">60S acidic ribosomal protein P2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003321</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">P1 and P2 exist as dimers at the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149625.1">
        <property type="nucleotide sequence ID" value="XM_037294244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9I6"/>
    <dbReference type="GeneID" id="59331732"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="62"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="11526" checksum="D5E78DB71A6134BD" modified="2022-01-19" version="1">MKHLAAYLLLGLGGNTSPSAADVKGVLESVGIDADEERLDKLIAELKGKDLQELIAEGATKLASVPSGGGGGGGAGGAGPAAGGAAPEAAEEKKEEKKEEEKEESDEDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9V5</accession>
    <name>A0A8H6F9V5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003458</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149761.1">
        <property type="nucleotide sequence ID" value="XM_037294380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9V5"/>
    <dbReference type="GeneID" id="59331869"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="274"/>
            <end position="356"/>
        </location>
    </feature>
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</entry>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBD3</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <begin position="356"/>
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    </feature>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A8H6FBF3</accession>
    <name>A0A8H6FBF3_9LECA</name>
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        <alternativeName>
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        </lineage>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBF3"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019313">
        <property type="entry name" value="Mediator_Med17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114:SF7">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10156">
        <property type="entry name" value="Med17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="37"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="603"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="610"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="626" mass="68912" checksum="D895908A7BCAD8B4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBL7</accession>
    <name>A0A8H6FBL7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151606.1">
        <property type="nucleotide sequence ID" value="XM_037292187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBL7"/>
    <dbReference type="GeneID" id="59329673"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="552" mass="64165" checksum="D1F874F37572EE8F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBY6</accession>
    <name>A0A8H6FBY6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152133.1">
        <property type="nucleotide sequence ID" value="XM_037291771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBY6"/>
    <dbReference type="GeneID" id="59329253"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034688101" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="8013" checksum="DF7CD571693E81B1" modified="2022-01-19" version="1">MIEAFQCCFALLFCVFQVYYSAHEPFRGNQNIPVIGTLTTSVVYLGAPLHDLDRLGCIYIEPRGGQLCRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC45</accession>
    <name>A0A8H6FC45_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cysteine dioxygenase</fullName>
            <ecNumber evidence="2">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_000728</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152027.1">
        <property type="nucleotide sequence ID" value="XM_037291665.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59329146"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10548">
        <property type="entry name" value="cupin_CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056485">
        <property type="entry name" value="ARM_KRIT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056015">
        <property type="entry name" value="DUF7593"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24521">
        <property type="entry name" value="Ank_KRIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24513">
        <property type="entry name" value="DUF7593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0883">Thioether bond</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034673742" description="cysteine dioxygenase" evidence="11">
        <location>
            <begin position="22"/>
            <end position="1787"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="840"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="873"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="domain" description="KRIT1 ARM-repeats" evidence="13">
        <location>
            <begin position="981"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7593" evidence="12">
        <location>
            <begin position="1588"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="526"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="902"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="931"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1126"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1426"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="529"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="561"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="589"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="627"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="637"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="705"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="728"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="931"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="957"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1130"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1148"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1197"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1273"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1297"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1382"/>
            <end position="1392"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1426"/>
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        </location>
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        <location>
            <begin position="1439"/>
            <end position="1466"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1467"/>
            <end position="1477"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1478"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1513"/>
            <end position="1588"/>
        </location>
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        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="338"/>
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        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
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        </ligand>
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        <location>
            <begin position="343"/>
            <end position="413"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF24521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6222681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1787" mass="198917" checksum="3FBD41F48F8125DA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCH6</accession>
    <name>A0A8H6FCH6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000909</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152204.1">
        <property type="nucleotide sequence ID" value="XM_037291842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCH6"/>
    <dbReference type="GeneID" id="59329327"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="365" mass="40210" checksum="69F345AB21467835" modified="2022-01-19" version="1">MCIGGSGFIASHILNTLLEHGQVTYHALNIQPVPDPNVNFSFGVVFTVRSESKGRRIIDVYPDTPSEKLSFVVVEDIAEEGAFDELSDFLKAVKSDPPFNFVIHTASPYHFNIQDSVKDFLEPAINGTTGIIKAVMAYAPTVKRVVLTSSFATIVNPWNHAKVYDETMWGPVTREQAMDPTGAYRGSKILAEKTAWELIETQKPQFDLVTIHPPMVYGPVPSFLNGLDSLNTSNHRIRAMIQGETKNTGLPPTAVYLFTDVRDVALAHVRALEVPQAGGQRYFVVGGHYSNKMIADTIRRSYPQLAERLPAVEDTEDDTPPNVYGYDNRKSREVLGLTYGSIEECVKDTVASMLALEAGQGSTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCI7</accession>
    <name>A0A8H6FCI7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000229</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152604.1">
        <property type="nucleotide sequence ID" value="XM_037291169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCI7"/>
    <dbReference type="GeneID" id="59328648"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF1">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="51383" checksum="C53B2B54D2C79773" modified="2022-01-19" version="1">MAKDSLETSDQFKMVEIRDDRQTRQQKSVEQDREDLARMGKKQVLKRQFGLMSMLGFGCIVLSTWEGVLALFVVGFTNGGPAGLVYGYLLTWIGTLSIFITLSELASMAPTSGGQYYWCAMLGPPQYGKVLSYITGWLTVFGWQASVISASYLTGTMIQGLLILNYADYDPKPWQAMLLMWACLLVAVLLNTLVSNFLPKIEGAILIFHVVGFFAILIPLVYLAPGHGPASEVFTTFLNEGGWGTQGLSFWLGVSSTVFAFLGADSVVHMSEEIQGASVNVPRAIIGSIAINGALGFGMLLAVLFTLGDVKAVLGTDYIYPFIQIFLQSNNSSGGTTAMTAVILALTFLSTIGLVATSSRMTWSFARDRGLPGWRYLSKNPLPSTEGSPELAWGPWRVPGFLGVVNNAYACIWMVIILFFSSWPKTTPTTASTMNYSIFITLFVAIVSGVYYVVWGRKTYTGPVVELALPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD48</accession>
    <name>A0A8H6FD48_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_010681</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.</text>
    </comment>
    <comment type="domain">
        <text evidence="12">The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the anamorsin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153167.1">
        <property type="nucleotide sequence ID" value="XM_037301533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD48"/>
    <dbReference type="GeneID" id="59339071"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000374">
        <property type="entry name" value="3-oxoacyl-(Acyl-carrier-protein) reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11000">
        <property type="entry name" value="Fe-S cluster assembly protein Dre2, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03115">
        <property type="entry name" value="Anamorsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007785">
        <property type="entry name" value="Anamorsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046408">
        <property type="entry name" value="CIAPIN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031838">
        <property type="entry name" value="Dre2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF111">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_1G10100)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05093">
        <property type="entry name" value="CIAPIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16803">
        <property type="entry name" value="DRE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="14">
        <location>
            <begin position="311"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="145"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fe-S binding site A" evidence="12">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6224107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="586" mass="62352" checksum="65FCA56F1CADD712" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDC1</accession>
    <name>A0A8H6FDC1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_000526</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152900.1">
        <property type="nucleotide sequence ID" value="XM_037291465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDC1"/>
    <dbReference type="GeneID" id="59328945"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22265">
        <property type="entry name" value="UDM1_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000139">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000705">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15413">
        <property type="entry name" value="PH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="84"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="CRIB" evidence="14">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="618"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="263"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="349"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="450"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="477"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6223683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="909" mass="99294" checksum="EC5240FF13D40BCF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDD2</accession>
    <name>A0A8H6FDD2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152931.1">
        <property type="nucleotide sequence ID" value="XM_037291496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDD2"/>
    <dbReference type="GeneID" id="59328976"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="522"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="587"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="730"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="974"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="373"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="534"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="600"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="730"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1012"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1035"/>
            <end position="1046"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1068" mass="116614" checksum="FDF8B95BE6B59B0E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDR7</accession>
    <name>A0A8H6FDR7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010708</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153194.1">
        <property type="nucleotide sequence ID" value="XM_037301560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDR7"/>
    <dbReference type="GeneID" id="59339098"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14474">
        <property type="entry name" value="SPX_YDR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="791"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="827"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="860"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="204"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="306"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="576"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="744"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="309"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="600"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="644"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="659"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="763"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="883" mass="97472" checksum="A5E422F04164A289" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE10</accession>
    <name>A0A8H6FE10_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010996</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153479.1">
        <property type="nucleotide sequence ID" value="XM_037301845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE10"/>
    <dbReference type="GeneID" id="59339386"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13437">
        <property type="entry name" value="SPFH_alloslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="3">
        <location>
            <begin position="87"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="314"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="357" mass="38581" checksum="733719154A57E782" modified="2022-01-19" version="1">MAAMDNGKAPLPANGTFRNGAPGGETNTRLINVQPPRREDLQPAYAQTLQGESEDAGSHGWYGSMINTLGSLVGCCGAIPCCIVCPNPYKPVSQGNVGLVTKFGRFYRAVDPGLVKINPLSEKLIQVDVKIQIVEVPKQVCMTKDNVNLQLTSVIYYHITSPHKAAFGISNIRQALVERTQTTLRHVVGARVLQDVIERREEIAQSIGEIIEDVASGWGVQVESMLIKDIIFSTELQDSLSMAAQSKRIGESKVIAARAEVESAKLMRQAADILSSAPAMQIRYLEAMQAMAKTANSKVIFLPATNQTVQQQLAQADTYGEGPSSQGQSSKSSTGPFMGDTNDGFQQAMNARVIENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEP6</accession>
    <name>A0A8H6FEP6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidylinositol 3-kinase VPS34</fullName>
            <ecNumber evidence="7">2.7.1.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009759</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154466.1">
        <property type="nucleotide sequence ID" value="XM_037300618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEP6"/>
    <dbReference type="GeneID" id="59338154"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08397">
        <property type="entry name" value="C2_PI3K_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00870">
        <property type="entry name" value="PI3Ka_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00896">
        <property type="entry name" value="PI3Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000009">
        <property type="entry name" value="Phosphatidylinositol 3-kinase VPS34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000238">
        <property type="entry name" value="Phosphatidylinositol 3-kinase VPS34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057756">
        <property type="entry name" value="PI3-kinase_type3/VPS34_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008290">
        <property type="entry name" value="PI3K_Vps34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000587">
        <property type="entry name" value="PI3K_Vps34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="11">
        <location>
            <begin position="32"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="10">
        <location>
            <begin position="365"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="9">
        <location>
            <begin position="632"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="157"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="230"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6225757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="915" mass="102990" checksum="7FCB6B06B44EC51B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFF8</accession>
    <name>A0A8H6FFF8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154714.1">
        <property type="nucleotide sequence ID" value="XM_037299888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFF8"/>
    <dbReference type="GeneID" id="59337422"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034179675" evidence="1">
        <location>
            <begin position="22"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="201" mass="22200" checksum="EAC3ECEA24B39C01" modified="2022-01-19" version="1">MTKYYRILLSLLLISSYLIDGAPTMAPISLPADDALSTYYCIDSPAWSEPSFLPKNCATAMSQFFVQEMLVHGDVVFEFHAVGAQPRSRYPSQHTPQKFTYEQLPGVPPATMFGLTDVASYSDAWNAVKRVKNNCISEYLATNETIHESGGGMNFRSLTGWSATGTLHLLRTLGESVADVKYVLQEAKALLVSSYGTQIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFN5</accession>
    <name>A0A8H6FFN5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Maintenance of telomere capping protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154682.1">
        <property type="nucleotide sequence ID" value="XM_037299856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFN5"/>
    <dbReference type="GeneID" id="59337390"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018814">
        <property type="entry name" value="DUF5427"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28265">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28265:SF1">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10310">
        <property type="entry name" value="DUF5427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="53520" checksum="6CAA86408A136658" modified="2022-01-19" version="1">MSKKEKLTDEELFAQFEGLEGDGTAVQAPKPATDGQQTDADLLEELGIPERPKPSSRPHTPRPQSTSTASGTRSSPKLTRAATPSSTDGARSSEEKARLRKSGDSTRSFHNSFTPASSGEGESEPEKSASFVPEAVQAQSSGGGWWGSVFSTASAAVKQAEALAKEIRQNEEAQRWAEQVKGNVGALRGLGGELRSRALPTFSNILHTLAPPISSHERLQIHITHDIVGYPSLDPLIYSTFSRVMAQVEGGDLMVIQRGQESTQRRGEDAGFTGSSSSGWSDGPWWRQATEARNLGAVKGLVEGTKLSKVSAESYATDYFAPRGGLEEAAKQATEVLSESNPVRSSDIFLAIQAISQTSPEDFFGGPTSQPKGEEGAVEPEEDDELISFAIYLHDPIHGITFNTVTQTLPQRWTEWLDASATSESAESGLPDEIAEIVNTGGVDPREWVAEWVEEVLSLGVGVVAQRYVARRMGVGLGGIGRGKARQEALESGGGEAARAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGD0</accession>
    <name>A0A8H6FGD0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acetyl-CoA synthetase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008319</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155187.1">
        <property type="nucleotide sequence ID" value="XM_037299186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGD0"/>
    <dbReference type="GeneID" id="59336716"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05911">
        <property type="entry name" value="Firefly_Luc_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF317">
        <property type="entry name" value="ADENYLATE-FORMING ENZYME AFEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="60"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="8">
        <location>
            <begin position="472"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="599"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="599"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6226878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="78967" checksum="81879A6ADA2E6529" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH56</accession>
    <name>A0A8H6FH56_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156044.1">
        <property type="nucleotide sequence ID" value="XM_037298708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH56"/>
    <dbReference type="GeneID" id="59336235"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="543" mass="60636" checksum="0074FAD9F548168C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH72</accession>
    <name>A0A8H6FH72_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="8">SDH assembly factor 3</shortName>
            <shortName evidence="8">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007885</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 8">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156089.1">
        <property type="nucleotide sequence ID" value="XM_037298753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH72"/>
    <dbReference type="GeneID" id="59336282"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="210" mass="23705" checksum="19EDC7B888E87378" modified="2022-01-19" version="1">MRVSIRLLAKPTAVGSTNGLKPAPLALLPPIPLYRRLLRAHRKYLDPQMRLLGDEYIKSEYRSHRNVENPMHIIGFLTEWQMYAQNIEGGSWKGEKIDKGKIDKMSDQQLGQLYELMQAIQKQAHEDGARNAAKYAQELAKEKTCPKCDKNDDYDGKKIRMVKATVHATKFGFGPNKSDCGINLGKKTRKYGPKKEPSTMLLAEGGCVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHJ6</accession>
    <name>A0A8H6FHJ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008261</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156464.1">
        <property type="nucleotide sequence ID" value="XM_037299128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHJ6"/>
    <dbReference type="GeneID" id="59336658"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000008">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF9">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT TAURINE DIOXYGENASE (AFU_ORTHOLOGUE AFUA_3G01010)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="87"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="376" mass="42216" checksum="F774F9E351789267" modified="2022-01-19" version="1">MAPTAIDPVILDVVQPKKDTLNLPTESRKRLEKAGIDLSSGYPYRPAKPLYLQDVQKIRDYHREHVDPGARADPAKRALFSAAEKVVDLTAHIGTEIVGLQLKDLTDQQKDELGLLIAERSVVFFRDQDISPQQQRSLGEWYGEVEVHPQVPQVPGVAGVTVIWPELQATEAKANFRNPGGASRWHSDLVHERQPAGVTHLHNDTVPTVGGDTLWASGYSAYEKLSPNFRKIIDGKKAVYRSAHAYLDRDDPTAGPKYVERVHPLVRVHPATGWKALWVNRAMTVRIVGLDKAESDLILGYLYDVYEKNVDIQVRFKWTPRTSALWDNRITIHNASWDYEGSQPRHGTRVTALAEAPYFQEDASSRREALGLAVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHK5</accession>
    <name>A0A8H6FHK5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156345.1">
        <property type="nucleotide sequence ID" value="XM_037299009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHK5"/>
    <dbReference type="GeneID" id="59336538"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000037">
        <property type="entry name" value="TBC1 domain family member 22A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000001">
        <property type="entry name" value="TBC1 domain family member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="284"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="603" mass="67933" checksum="F67A92B03586A59A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHR6</accession>
    <name>A0A8H6FHR6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sphingolipid long chain base-responsive protein LSP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008034</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156238.1">
        <property type="nucleotide sequence ID" value="XM_037298902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHR6"/>
    <dbReference type="GeneID" id="59336431"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:endocytosis"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
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        <property type="entry name" value="AH/BAR_dom_sf"/>
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    </dbReference>
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        <property type="entry name" value="PRIMARY COMPONENT OF EISOSOMES (EUROFUNG)"/>
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    <dbReference type="PANTHER" id="PTHR31962">
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    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
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    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="227"/>
            <end position="295"/>
        </location>
    </feature>
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        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="32525" checksum="3389BC5EF34565E6" modified="2022-01-19" version="1">MASLRGMQQPELSKKLFKIIKSENHAIGAYESASRERISIASQLSDWGEATGDDSVSDVSDKLGVLLSELGEQEDVFAQNLEDYRGILKQIRNTESSVQPSRIHKAKISDEIAKLKYKEPESTKIVQLEQELVRAEAQNLVAEAQLTNITRQKLKEAYDLHFAATIERAEKQIMLARHGRRLLNLLDDTPVVPGDTRPAFEHAHQARQVLNDAEEDLRDWAPNLEPIHSSATEMGSNLMPPQAGTADTSVSGQEEESMIRAGESSTGQLEAPAPPEHRGYGESSTVAQPEAASSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI75</accession>
    <name>A0A8H6FI75_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007128</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037298021.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59335528"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027040">
        <property type="entry name" value="PSMD4"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="F-BOX AND WD DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G11400)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034627195" description="F-box domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="2469"/>
        </location>
    </feature>
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        <location>
            <begin position="1466"/>
            <end position="1512"/>
        </location>
    </feature>
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        <location>
            <begin position="53"/>
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        </location>
    </feature>
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        <location>
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            <end position="1436"/>
        </location>
    </feature>
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        <location>
            <begin position="1571"/>
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        </location>
    </feature>
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        <location>
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        <location>
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            <end position="2375"/>
        </location>
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        <location>
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            <end position="2438"/>
        </location>
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        <location>
            <begin position="2285"/>
            <end position="2312"/>
        </location>
    </feature>
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        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
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        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
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        <location>
            <begin position="498"/>
            <end position="508"/>
        </location>
    </feature>
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        <location>
            <begin position="518"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="597"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="649"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="681"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1328"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1348"/>
            <end position="1361"/>
        </location>
    </feature>
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        <location>
            <begin position="1362"/>
            <end position="1375"/>
        </location>
    </feature>
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        <location>
            <begin position="1393"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1575"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2341"/>
            <end position="2350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2361"/>
            <end position="2372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2412"/>
            <end position="2428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2469" mass="258221" checksum="06C41144C89FB241" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKC8</accession>
    <name>A0A8H6FKC8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004444</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157362.1">
        <property type="nucleotide sequence ID" value="XM_037295361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKC8"/>
    <dbReference type="GeneID" id="59332852"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15670">
        <property type="entry name" value="ePHD_BRPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15492">
        <property type="entry name" value="PHD_BRPF_JADE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000008">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000007">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF107">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="423"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="477"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="388"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="937"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="975"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1082"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="938"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1009"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1038"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1084"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1119"/>
            <end position="1134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6230105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1134" mass="125938" checksum="36976BACD2983F6E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKH9</accession>
    <name>A0A8H6FKH9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA-binding protein VTS1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">RNA-binding protein vts1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_004548</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">RNA-binding protein involved in post-transcriptional regulation through transcript degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer. Binds to RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VTS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157466.1">
        <property type="nucleotide sequence ID" value="XM_037295465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKH9"/>
    <dbReference type="GeneID" id="59332956"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09556">
        <property type="entry name" value="SAM_VTS1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000033">
        <property type="entry name" value="Protein vts1, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050897">
        <property type="entry name" value="SMAUG/VTS1_RNA-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057327">
        <property type="entry name" value="Vts1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037635">
        <property type="entry name" value="VTS1_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515:SF5">
        <property type="entry name" value="PROTEIN SMAUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25479">
        <property type="entry name" value="Vts1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SAM" evidence="14">
        <location>
            <begin position="543"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="157"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="246"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="510"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="302"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6230209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="607" mass="65423" checksum="273DA9088097730A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKR2</accession>
    <name>A0A8H6FKR2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DSC E3 ubiquitin ligase complex subunit 3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004673</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157590.1">
        <property type="nucleotide sequence ID" value="XM_037295589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKR2"/>
    <dbReference type="GeneID" id="59333080"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044695">
        <property type="term" value="C:Dsc E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045226">
        <property type="entry name" value="Dsc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025390">
        <property type="entry name" value="Dsc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019413">
        <property type="entry name" value="Dsc3_ub-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28049:SF1">
        <property type="entry name" value="DSC E3 UBIQUITIN LIGASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28049">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN YOR223W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13373">
        <property type="entry name" value="Dsc3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10302">
        <property type="entry name" value="Dsc3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="DSC E3 ubiquitin ligase complex subunit 3 ubiquitin-like" evidence="3">
        <location>
            <begin position="14"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="DSC E3 ubiquitin ligase complex subunit 3 C-terminal" evidence="4">
        <location>
            <begin position="154"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6230333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="29171" checksum="1DA4CD4BC65BA7F1" modified="2022-01-19" version="1">MPPTPPPPPTPPLTLIIRFTTSLPDLPLPIVHPRATTPTTLATLIRPHLPPALAACPLRLIHAGALLPPTAPLHASLRLSAAGPPPARLYVHCSIATGARLSPAELDAEAESAAVALSQHDTAGRDDRASSSAPARTAADDDDDYRPTLPPPAQGFDRLLSAGLSAAEIAALRSQFLAVQAHTHTPDAMPSGAELRALEERWLDAGTPLDGGGGADGDEEGGGGGGLEDMLWGNVMGFFWAVGAIVWLVREEGVWTKRRQVGVVTGVLVNIAFCMLRVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKW5</accession>
    <name>A0A8H6FKW5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004715</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157630.1">
        <property type="nucleotide sequence ID" value="XM_037295629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKW5"/>
    <dbReference type="GeneID" id="59333121"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="188" mass="21481" checksum="4D9BCFA3A521B1F7" modified="2022-01-19" version="1">MNNPQERHNNVHFLAIADMATPTSASPQTLADSAPQRTQHNSIYTPRGLFNQVTLPNPSHLQPSLAKRRTKADQRLLSEFGLSKNTPMRRSSHNAMGKRMFFLLSGHHHMQKVVPPSYEHALQLDKKALLVSQYRLPSFREGFSDWIQDTSLMDPMQSEITVDRLSRGQENSGLHLPSIVTLLKREWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYA2</accession>
    <name>A0A8H6KYA2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156663.1">
        <property type="nucleotide sequence ID" value="XM_037298028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYA2"/>
    <dbReference type="GeneID" id="59335535"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="182"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="330" mass="35911" checksum="90F1ECCE69D0BBEB" modified="2022-01-19" version="1">MLQLPTIKVLDIEILTDWDFENVKAFGDSIPGFRSSSTFLTTLRSNLHLASDLSPLNSLVSTPTTPAFAISHLPQPSSLSITTTPSKNSLLKLDPKPEPILSTALALPGAPEDRSAALRLIADSVVQQRQLAAKELVLHPATLAVTVLVIAVLAQFCEWRPLFTAAAGLTVAALAAIYWITIDYATLAERINWDWLGGYTPPSTPTGERGSGLKKQSKCDDPVVLVSKWGEEVIGALIMKVVKRERKAYVRAWTVDASCRGKGIGVGLLEEGVRVAWGKGARAFEFERGHANSHRVLPPLFNGGFEKRDATARVVLADVVKEIRRERSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYE5</accession>
    <name>A0A8H6KYE5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156649.1">
        <property type="nucleotide sequence ID" value="XM_037298014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYE5"/>
    <dbReference type="GeneID" id="59335521"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052769">
        <property type="entry name" value="TPR_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46014">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46014:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="28999" checksum="D73BD1221F0CDCDA" modified="2022-01-19" version="1">MALPDPSPDLPDESEASLVPRFSQEEEAALLQESNAQKSTANKFFTSSQYSQAIGEYDKALSSCPNYLEYEIAVLKSNIAACHLKLEDWKAAVESATTALDALDRLLPKKKENKEDDAEKDGGVVEIEGEGAEAEEELKKLEMSDQRRDDILRIRSKALMRRAKANSEQGGWGNLQGAEDDYKALAQLPTLPPQDQKIVQAALRSLPPRINTAKEREMGDMMGKLKELGNGILKPFGLSTDNFKMDKDPNTGSYSMNFNQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZM8</accession>
    <name>A0A8H6KZM8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157356.1">
        <property type="nucleotide sequence ID" value="XM_037295355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZM8"/>
    <dbReference type="GeneID" id="59332846"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="245" mass="26442" checksum="DF26B44F6AD446A8" modified="2022-01-19" version="1">MRVPDIAVTYTVVKNSTLYVNGGRETFIDVKANGSSSEQFGNITGLQTGTAPPVLSRGALFYSSANDSNIYLWGGTTSYSNTSFPGYEAPIPQEYSLWLFDISTQGSVQYDLTLGSANRPSYGSFTNAEELGLGFYFNGELDSGCEIQTQIYGDGVKHLIGGMIVVDLIAGKQQNVANTTGIISPHIENLLPMDEIDIFDVASYYNDSTPHGTWYKQTTSGATPDPRIDLYLILASAPDGSSHNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZX2</accession>
    <name>A0A8H6KZX2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157610.1">
        <property type="nucleotide sequence ID" value="XM_037295609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZX2"/>
    <dbReference type="GeneID" id="59333101"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100">
        <property type="entry name" value="IMP2'P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100:SF4">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="311"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="537" mass="58492" checksum="1044158371D8A26A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L054</accession>
    <name>A0A8H6L054_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004896</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157809.1">
        <property type="nucleotide sequence ID" value="XM_037295808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L054"/>
    <dbReference type="GeneID" id="59333302"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051414">
        <property type="entry name" value="Adenylate-forming_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439:SF2">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439">
        <property type="entry name" value="PHENYLACETATE-COENZYME A LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="4">
        <location>
            <begin position="563"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="5">
        <location>
            <begin position="680"/>
            <end position="919"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1053" mass="117037" checksum="06EB6B164617E843" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6G6</accession>
    <name>A0A8H6C6G6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006884</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146981.1">
        <property type="nucleotide sequence ID" value="XM_037297780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6G6"/>
    <dbReference type="GeneID" id="59335284"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="397" mass="45456" checksum="AB5E66B26069EC1C" modified="2022-01-19" version="1">MADSKISSEAGSRFLGVPLEIRRQIYRILLLDPPESFLSTPCMPWYQQGVFQAKVKKDGTDNEMWHGTEYQGNFYEHNYNESDSDVDFPFCDSSSKTRIAEAPRLGESKLQNGHNSVQKDGADQEMNVQRFPAILRTNRQIYSEASPLLYRELNMRLQPGDVLCMKSSKDIVKASERVWRHNPLQDTGVLKPNGLTNYAEPELDGVMDPHVLARFKKITFDLDINWELETLDAETGRQTAGDTTLQDQTAPSLFVNDNMTVNSQDEARLLAFYRRSTILHQLVKILSNSPDVVRLQILFDIEVLARYDMSSDSDSEDEQEDKDPARKMIAANERAMELFLDSGLLAPLEKLSNVRSFEFEYAALNCDCEHYKPKPKYARMLSDLKQNIERNYAVKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6N9</accession>
    <name>A0A8H6C6N9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006282</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147305.1">
        <property type="nucleotide sequence ID" value="XM_037297180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6N9"/>
    <dbReference type="GeneID" id="59334683"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF52">
        <property type="entry name" value="ADIPONECTIN RECEPTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6217870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="334" mass="37909" checksum="03291F41EBE0D4B8" modified="2022-01-19" version="1">MARPRKMASSKEAPQTPITAKGARRLKSWHEIPSWQQDNEYILSGYRQATGSFKSCCESLGYVHNETVNIYSHVIGAATFLTAPIYYYRALYLRYPLATQADVFVSSTFFYGVSICFILSATYHIISNHSPEVQRFGNQLDYLGIVILMWGSTIPSIYYGFYCDSRLQKIYWANVSTLATLCVVATLHPRFRHPDIRPYRAAMYAGLGLSAVVFVMHGILLHGWTVQNRRMSLDWMGLVALFNLMGAVIYAARVPEKLRPLRYDIYGNSHQVLHVAVVLAGLAHMFGLSRAFDYLHTHGSFLDVSYSAKCTNSTNKDKYSIDLESSRRRTIFMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Q5</accession>
    <name>A0A8H6C6Q5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Luciferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147243.1">
        <property type="nucleotide sequence ID" value="XM_037297533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Q5"/>
    <dbReference type="GeneID" id="59335036"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048273">
        <property type="entry name" value="Luciferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040841">
        <property type="entry name" value="Luciferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38695">
        <property type="entry name" value="AMINO ACID PERMEASE_ SLC12A DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38695:SF1">
        <property type="entry name" value="AMINO ACID PERMEASE_ SLC12A DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17648">
        <property type="entry name" value="Luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Luciferase" evidence="2">
        <location>
            <begin position="192"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="283" mass="30794" checksum="5CE10232EB0E127F" modified="2022-01-19" version="1">MAAVLDQGVKNFTDILTNPNSPSKLSYLLYPGLLITILILPAYRFVLKDYHVFLALGPGGTPKTFPGYLRVSYLRLFTISDPFRPPSLAETVFPTNGYLRQLPQRSGPRPTVAGIAPHRQLDQKCTAELHHVLRDGLHKLAAAFPSLIRKGSSCFEKQGLALFLSACTQSAPPNDAAHPGFSHLNPTCQDTGEICHLHAMDSSMHLTLHPLDISIVLSAGWGERHPLAGKYVHGKELLPSGFVMVYAPRQESQVETVMEIVKAGAWWVGGVDLAKANGLGGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6U8</accession>
    <name>A0A8H6C6U8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006482</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147505.1">
        <property type="nucleotide sequence ID" value="XM_037297380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6U8"/>
    <dbReference type="GeneID" id="59334883"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14812" checksum="E6F157F8F631023F" modified="2022-01-19" version="1">MVKTSVLNDALNAINNAEKSGKRQVLIRPSSKVIVKFLHCMQKHGYIGEFEEVDDHRSGKIVIQLNGRINKTGVISPRYNVRITDFEKWVVKLLPSRQFGYIVLTTSAGIMDHEEARRKHVAGKIIGFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C702</accession>
    <name>A0A8H6C702_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dual-specificity kinase</fullName>
            <ecNumber evidence="1">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_006258</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147281.1">
        <property type="nucleotide sequence ID" value="XM_037297156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C702"/>
    <dbReference type="GeneID" id="59334659"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14134">
        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000612">
        <property type="entry name" value="Serine/threonine-protein kinase AFC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="218"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6217846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="593" mass="67646" checksum="0B33DAF78D16DB32" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C798</accession>
    <name>A0A8H6C798_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147702.1">
        <property type="nucleotide sequence ID" value="XM_037297128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C798"/>
    <dbReference type="GeneID" id="59334630"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22355" checksum="6013AA5409421842" modified="2022-01-19" version="1">MPFIVSEDIADEDFDSLFAIQYKAFSNQPALKACSPEGLDNPGRSENVARFINVLGWKESNVVAAKVVDDETGEICAFATMRVCDGNPFAGAKDSDIHCPQVDEGMRSAVEWTLNTKNDRCATLLVKWAARIIDEKGSRAMVEASKDATQYGLYSKQGFRIVDTYDYIDEQKFPGFEGMYVVPWSEMPKTDRNLVRLWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7W1</accession>
    <name>A0A8H6C7W1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148043.1">
        <property type="nucleotide sequence ID" value="XM_037296862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7W1"/>
    <dbReference type="GeneID" id="59334364"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="37893" checksum="B488685CC3D97725" modified="2022-01-19" version="1">MVGTLKPAPYWRQRLVLYRPPPIPSPSLTVLAQASTLLYSQFFVKPAIPAHSFSGQTIIVTGSNTGIGHEAAIHIVRLGQKGRAGVVVAWELHLARYAFVKAFVHRIKGLEGLDAALQYMGKSSHEFELAEIDEYVVRLPINDHQRPPAKESRPSITIKVVCTFLLLELRSSAQKFHTTPRISIVRSSVHNLIELPAKADPEMFNALNDRNSAVAKLSTRYIDGKPLEILCSRGLASTLASALSSSRKPVVILSLLNPGFCASASFQNPSTAFKLQVKALGRTAEEDSRAVVDAVASGKDSHGQHLSDSGIAKVSDFVLSRKGWETQERAWDGLNASLEKTRVLDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C832</accession>
    <name>A0A8H6C832_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148040.1">
        <property type="nucleotide sequence ID" value="XM_037296859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C832"/>
    <dbReference type="SMR" id="A0A8H6C832"/>
    <dbReference type="GeneID" id="59334361"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="203" mass="22922" checksum="F91BDCA42E0B65C1" modified="2022-01-19" version="1">MATPRAGFHREFDPTFPSPDLTVCSLCGLDPCDWDSDNPFDFHATRSPECPFVRATQQKKKHRNQKTRQQYTRRSLQPVTQSATQQATQSSTQSATQQATQSDTLQEVELQQANAENTAENTAEKSSGSKAFSIKNTAHLSTIPRHSNHYDIDAENDTAIWPLIPTALMWYITAMSTQEKDLLRRSQPIGPLQARVTYRTMHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8L5</accession>
    <name>A0A8H6C8L5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transglutaminase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148342.1">
        <property type="nucleotide sequence ID" value="XM_037296360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8L5"/>
    <dbReference type="GeneID" id="59333856"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140278">
        <property type="term" value="P:mitotic division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.620.30:FF:000005">
        <property type="entry name" value="SH3 domain protein (Cyk3), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052557">
        <property type="entry name" value="CAP/Cytokinesis_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056409">
        <property type="entry name" value="Ig_CYK3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46333">
        <property type="entry name" value="CYTOKINESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46333:SF2">
        <property type="entry name" value="CYTOKINESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24584">
        <property type="entry name" value="Ig_CYK3_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="2">
        <location>
            <begin position="709"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1046"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="530"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1046"/>
            <end position="1059"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1128" mass="122204" checksum="A9E22DF732207ED1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8P8</accession>
    <name>A0A8H6C8P8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Regulator of volume decrease after cellular swelling-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005288</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148181.1">
        <property type="nucleotide sequence ID" value="XM_037296199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8P8"/>
    <dbReference type="GeneID" id="59333694"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039924">
        <property type="entry name" value="ICln/Lot5/Saf5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21399">
        <property type="entry name" value="CHLORIDE CONDUCTANCE REGULATORY PROTEIN ICLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21399:SF0">
        <property type="entry name" value="METHYLOSOME SUBUNIT PICLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03517">
        <property type="entry name" value="Voldacs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="203"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="273"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="310"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="34266" checksum="E43D309BC1AACF4C" modified="2022-01-19" version="1">MEIIHQSPTSSSFTPLAVHQSQTPDSFYSGPAVLYHHSPSASLLLHASDLAAAPALAALANGAHRSANGTATVAVNGHDAAEDEDEDEEFEIAGVDVWVTSERFILYSPTLSTGLSIPYPSISLHAVQRPSHGNPASLFLQLLTEAPTFDDHDPDSTISMTIVPAAAAAATTSEDSPEQVGRVTPESESQARRLYAALSACANLHPDPASGSEGDGEDGEGGGGQRPAVMFEGDQDVGGVYPLSNGEGGGAGLPPPMPGSGVWITAENMGEFFDDEGNWRGNGGAGQEVGVSGGAGGLGEGAGTVRTREEDVDGEDAEGEGEGSEETKWRRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8W2</accession>
    <name>A0A8H6C8W2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005379</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148271.1">
        <property type="nucleotide sequence ID" value="XM_037296289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8W2"/>
    <dbReference type="GeneID" id="59333785"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF24">
        <property type="entry name" value="TRANSPORTER, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="39532" checksum="461B90C50412D060" modified="2022-01-19" version="1">MSSNPSPESVASGNTADPGIAPRQVVQRADGTDVELVSFPPGDPKNPRNWPTWRKWSIVIAITLIDLTVSFTASGFSPAAEKFAKDFGVSGEVGTLGLSLYYGRSPIYIISYGVFLFFVLGTALVHNLGGFLVLRFLSGWFSAVTIANFGGTIADIFEPHETGPAMSLFLWAATVGSPLGYFLFSCIAQYKGWRDIFWALLGICGGLWLIMTATLKETRHSVLLLRRAARERKQRGTDAIEVPDSMKQRGPRKLFKTALLRPFRFLFTEAIIFFGALYNGYLYGLSFLFNGAFSLVFGMGGHGFDVLGVGLTFLGIVVGISIGPITNIWQERYYQRRIAQVGGKNVPEARVQLGRIAGISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8X8</accession>
    <name>A0A8H6C8X8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148353.1">
        <property type="nucleotide sequence ID" value="XM_037296371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8X8"/>
    <dbReference type="GeneID" id="59333867"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="779"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="821"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="921"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="701"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="958"/>
            <end position="981"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1068" mass="117461" checksum="27B115149506D951" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C912</accession>
    <name>A0A8H6C912_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005492</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148383.1">
        <property type="nucleotide sequence ID" value="XM_037296401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C912"/>
    <dbReference type="GeneID" id="59333898"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11060">
        <property type="entry name" value="CYP57A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000050">
        <property type="entry name" value="Cytochrome P450 monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF232">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="50378" checksum="B9B08AACA8C2D838" modified="2022-01-19" version="1">MLSFADPRALKTIYGLNKGLTKSEFYPVQQAISNGDRLPSLFSTTDNQFHAELRRCVNSAFSMSSLVQYEPSVDIVTEMFLDQTEALFSSKNAICNFAEWLQFLAFDVIGQITYSKSHGFVDRGEDVDGMVGYLGKLFSYVAPVGQLPMLDRFFLKNPLLRFLDKHGIMSFTFPVVTFAKARINERLREIEALKAHGIDTVATVTGRRGDLLSMFLKAKEDRPAFFHDGRVLTMAVSMAFAGSETTAISLAAVFYYLLKNRPCYVRLMEEMDEAIHTGMVENRPNGLVTWTESQKLPYLDACIKEAFRLHPAAGLPLERVVPADGMEICGQLIPGGTIVGCSAWVIHRRPEIFGEDVETYRPERWLEANEESKKSMEGCMIQFGMGARTCIGKNISLLEIYKLVPSFLRRFEVDLANPNKEWRLHNAWFVKQLDFNTTFRPRSMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9A6</accession>
    <name>A0A8H6C9A6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">L29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005216</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148825.1">
        <property type="nucleotide sequence ID" value="XM_037296127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9A6"/>
    <dbReference type="GeneID" id="59333622"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="8">
        <location>
            <begin position="72"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00080124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="149" mass="16949" checksum="BE8BF38275BDF6EE" modified="2022-01-19" version="1">MPTRLTQTRKHRGHVSAGHGRVGKHRKHPGGRGMAGGQHHHRTNIDKYHPGYFGKVGMRYFHKLQNQFWKPVINLDKLWSLVPVEKRDEYLSKEKGDTAPVLDLLPLGYSKVLGKGRIPDIPMVVRARFFSREAERKIKEAGGVVELVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9B3</accession>
    <name>A0A8H6C9B3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005183</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148793.1">
        <property type="nucleotide sequence ID" value="XM_037296095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9B3"/>
    <dbReference type="GeneID" id="59333589"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000015">
        <property type="entry name" value="Aureobasidin resistance protein Aur1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF11">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE CATALYTIC SUBUNIT AUR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="213"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="385"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="431" mass="47863" checksum="A6E79EE815F4D60E" modified="2022-01-19" version="1">MRKSTFPSRNDFKKTYSKILLPFSWAPSIPTIIPHRIRRKLRSKVRSRQSPSTSLGHLETSYSPADTIESLRVHRWSFYDGQYLILIVLGIFSLCIIPSPGPLIKTALATLIIAALILPVTRQFLLPSLAIWSWLVFFYACGFIPSTWRPPIWVRVLPALENIFYGANLSNILSAHTSTFLSIMAWLPYGIIHFGAPAVCSIIMFVFAAPGTVPMFAKSFGWMNLIGVAIQLTFPCSPPWYENMYGLAPANYSIHGSPAGLAAIDKLFGIALYTPGFTGSPMVFGAFPSLHAGSAVMEALFMSHVFPRLTPLFALYTLWLWWATMYLSHHYAVDLVGGSLLSGVIFHVVKSKFLPRQQPNKFLRWDYDFVEVGDDPAEKGYISLDSGTEDADDWTVGSSSSVSSDSRSPIDESDSWEGATLASHSDSEAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Q2</accession>
    <name>A0A8H6C9Q2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148884.1">
        <property type="nucleotide sequence ID" value="XM_037294837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Q2"/>
    <dbReference type="GeneID" id="59332326"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="340" mass="38810" checksum="E8E317941073BAE8" modified="2022-01-19" version="1">MNFADLCINRFLPHCTNAIHNNAEAPLISLKLLLLLANKDRYCTLAQHHPLLKRDQTSMDSTRKNTIEDQPKTPCKDGIFQLVDMAGDRELTEKKRSRNLATAVREQTFAGLLEFTRKLAVIKLTFLTLGGNMEEYKARAGKIGTEEEQAHLAHLWQLYVELEESLPTKQEQHERAVEQENWAQREVEYHERLSTWRDTRMHALFADINSRLDDIVVYLTCKEEETSSEIPEGKAETKDDTSQGKTEAEDDTPEEKAETKDDTPHGDTEAEDDPPLDKAETEDDIRVKALFAEHDRVQAQIKSLGTYPEFKAQNDAFFKSHDEGKAQRLGDGLNEVQETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9X6</accession>
    <name>A0A8H6C9X6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Exonuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_005186</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148796.1">
        <property type="nucleotide sequence ID" value="XM_037296098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9X6"/>
    <dbReference type="GeneID" id="59333592"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09908">
        <property type="entry name" value="H3TH_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09857">
        <property type="entry name" value="PIN_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000011">
        <property type="entry name" value="exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000002">
        <property type="entry name" value="Exonuclease 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037315">
        <property type="entry name" value="EXO1_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044752">
        <property type="entry name" value="PIN-like_EXO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF65">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE PROTEIN DIN7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="15">
        <location>
            <begin position="138"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="481"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="523"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="629"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="723"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="535"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="581"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6219361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="759" mass="83548" checksum="4546E2F950DBCA2D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB33</accession>
    <name>A0A8H6CB33_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Translocator protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003379</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TspO/BZRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149682.1">
        <property type="nucleotide sequence ID" value="XM_037294301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB33"/>
    <dbReference type="GeneID" id="59331790"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033013">
        <property type="term" value="P:tetrapyrrole metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15904">
        <property type="entry name" value="TSPO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.100:FF:000001">
        <property type="entry name" value="translocator protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="entry name" value="TspO/MBR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004307">
        <property type="entry name" value="TspO_MBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057">
        <property type="entry name" value="PERIPHERAL-TYPE BENZODIAZEPINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057:SF0">
        <property type="entry name" value="TRANSLOCATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03073">
        <property type="entry name" value="TspO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="19497" checksum="0E09C57FC909ADDC" modified="2022-01-19" version="1">MALGTSIGFIVSPKESQNTYMALKQPPYRPPPQVFGPMWTALYGLMGYSAYRAWDTGMSSLNPNVVALTKQGATLFTIQLGLNLIWMPLFFRLKRPIEATADIVALTGVTGYLTYIWSKVDPVAAWGLAPYLGWLGFATYLSAGTGYLNGWDFSDKETDAPTDRGSGASTKYVNEKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBB1</accession>
    <name>A0A8H6CBB1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bacteriophage T5 Orf172 DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149709.1">
        <property type="nucleotide sequence ID" value="XM_037294328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBB1"/>
    <dbReference type="GeneID" id="59331817"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053006">
        <property type="entry name" value="Meiosis_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018306">
        <property type="entry name" value="Phage_T5_Orf172_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28094">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 113 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28094:SF1">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 113 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10544">
        <property type="entry name" value="T5orf172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00974">
        <property type="entry name" value="T5orf172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacteriophage T5 Orf172 DNA-binding" evidence="2">
        <location>
            <begin position="580"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="729"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="761" mass="85075" checksum="236069E19F4E3AF1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBT1</accession>
    <name>A0A8H6CBT1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150098.1">
        <property type="nucleotide sequence ID" value="XM_037294020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBT1"/>
    <dbReference type="GeneID" id="59331508"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="59" mass="6633" checksum="DFB9E512C204E30D" modified="2022-01-19" version="1">MVKYLEEQDAYKSFLVGGHLARRLNIEEAGSKAERARNTLGGDKDHVTKIDCMPEKAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBV6</accession>
    <name>A0A8H6CBV6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150024.1">
        <property type="nucleotide sequence ID" value="XM_037293946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBV6"/>
    <dbReference type="GeneID" id="59331434"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="82"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="233" mass="25176" checksum="E7B6AD257E32E4AF" modified="2022-01-19" version="1">MAPPRSLLAQTVIPILTRQPVLAAPPRFFRRHHHTHLLLRRPLPSPAPLTPRHRHHHSTEPSAPRPYTFEEVRALTADPSPSPSRTIIIDVREPAEVQESGRIPGAYSMPLTSNPDAFHLSDGDFFDRFGFGKPHAYKTSTSELPYSAEAKGREAGGGLGAEDGVEGADADGEAGDGGEDGGGTGVEEVVFYCKAGVRSRAAARMAREWVGVQAGDMKGGWIEWEGRGGEVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC13</accession>
    <name>A0A8H6CC13_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002964</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149966.1">
        <property type="nucleotide sequence ID" value="XM_037293888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC13"/>
    <dbReference type="GeneID" id="59331376"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF234">
        <property type="entry name" value="MFS-TYPE TRANSPORTER DBAD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="173"/>
            <end position="198"/>
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    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="210"/>
            <end position="229"/>
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    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
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        <location>
            <begin position="265"/>
            <end position="291"/>
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            <begin position="303"/>
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    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="38657" checksum="C8AA5562C739B543" modified="2022-01-19" version="1">MTDPKSCQNISLLNSFGVYQAYYSPAFLSNKSSSAISWVCTIEAFLLEIIGVIIGPIFDRGYFHTLVVLTQGGCVGLGSGLIFVPSVAVGTAAFSEKRAIAVGIASSASNLGGIIYPITFRQLQPRVGFAWATRTIAFIALRTFIISFAALRDHKVAGKEPRALIDVRAFRELPFMIYLLALFGLYAGYFVPLFYVATYASTHLYTTSDFAFYLTAVTTAGAFIGRLFPGLFLKPFAAIETLVLASAAAEISVLAWVGVYNLAGIIVFCVVFRLLSGIIITLTTVMVPVLSPGTLQGKVGTRLGMAYAGCGMGVLICSPIAGAASRTTVGDFRGAQIWGGATLLPGSALLVYPWSIVKRRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCB7</accession>
    <name>A0A8H6CCB7_9LECA</name>
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        <recommendedName>
            <fullName evidence="12">Pre-mRNA-processing protein prp40</fullName>
        </recommendedName>
    </protein>
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        <name evidence="10" type="ORF">HO133_002616</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
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            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037293541.1"/>
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    </dbReference>
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        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:RNA binding"/>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRP40 PRE-MRNA PROCESSING FACTOR 40 HOMOLOG A (YEAST)"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
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            <begin position="570"/>
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        </location>
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        <location>
            <begin position="344"/>
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            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
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            <end position="129"/>
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        </location>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="768" mass="89759" checksum="80631DDF7043E412" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCM7</accession>
    <name>A0A8H6CCM7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="GeneID" id="59331085"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="221"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="700"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="761" mass="87305" checksum="B655C23818A9657C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCS4</accession>
    <name>A0A8H6CCS4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
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            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150489.1">
        <property type="nucleotide sequence ID" value="XM_037293660.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59331147"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039712">
        <property type="entry name" value="Meu6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039483">
        <property type="entry name" value="Meu6_PH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42073">
        <property type="entry name" value="MEIOTIC EXPRESSION UP-REGULATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42073:SF1">
        <property type="entry name" value="MEIOTIC EXPRESSION UP-REGULATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15406">
        <property type="entry name" value="PH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="82"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="493"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="578"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="707"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="720" mass="74875" checksum="81A848831E0015FD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCT9</accession>
    <name>A0A8H6CCT9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Golgi SNAP receptor complex member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_002639</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of several multiprotein Golgi SNARE complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the GOSR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150393.1">
        <property type="nucleotide sequence ID" value="XM_037293564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCT9"/>
    <dbReference type="GeneID" id="59331051"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048219">
        <property type="term" value="P:inter-Golgi cisterna vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023601">
        <property type="entry name" value="Golgi_SNAP_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094:SF2">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094">
        <property type="entry name" value="GOS-28 SNARE- RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027109">
        <property type="entry name" value="Golgi_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="47"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR027109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6220958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="227" mass="25441" checksum="37FE689DD2B9436F" modified="2022-01-19" version="1">MSSSTGNGWAQLRQQTRTLESQTEGYFHKYSQFSQATAIPPKPTEDEKQTEATIQDLLEKREALITQLTRLLDSESPLTTSALKSNNLTRHREILLDHRRELVRLKASITDARQRANLLSNVRSDISAYRNSNPADAEADYMLDERNRIDRSHTVADGVLSQAYAINESFGTQRETLASVNRRIVGAASQVPGLNSLIGRIGAKKRRDGIILGSFIAFCCLLLFFLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDH4</accession>
    <name>A0A8H6CDH4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cobalamin-independent methionine synthase MetE C-terminal/archaeal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150606.1">
        <property type="nucleotide sequence ID" value="XM_037292953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDH4"/>
    <dbReference type="GeneID" id="59330439"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43844">
        <property type="entry name" value="METHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43844:SF2">
        <property type="entry name" value="SYNTHASE, VITAMIN-B12 INDEPENDENT, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12060)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE C-terminal/archaeal" evidence="1">
        <location>
            <begin position="242"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="465" mass="53082" checksum="4BB3D91B2F0B0C1F" modified="2022-01-19" version="1">MRGADPSKCRRLLFHIGTPHPTSLPVLLNIRPIVCLKGYHVLQILLLPFAICGPRIPVQSTLTMASNLHRDPPFRAEHLGSLLRPDNLLQTRADLDNGKTQQEQLTSIEDTSVKDIVDTQVKLGFHPISDGEYRRHMFWGTFFPGLEGFKEIKNPDVDIFRMYMPDIAAFTESGHKPGESVICTGKIKHVGSTYVDQWNYLKSIVPKERVHECKITLAAPEWYHMRYKEGRAYPKDVYPSDKEYFADIANAYQTELQILYDNGLRNVQIDDPNLAYFCSEKMLEGWKKDPLNTKTTDQTLDEYIDFYNDCISKRPADMHVGVHICRGNFVNSRHFSEGGYDRVAKKLFQDMHVNTYYLEYDTPRAGGFEPLKSLPKNKNVILGVITSKFPEMEDMEEMKKRVHEAADFVAEGNNQTRQEALQRLGVSPQCGFASHSAGNSVKKHDMVNKLTLVRELADSIWPGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDV4</accession>
    <name>A0A8H6CDV4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chromosome segregation in meiosis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001696</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays an important role in the control of DNA replication and the maintenance of replication fork stability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CSM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151163.1">
        <property type="nucleotide sequence ID" value="XM_037292624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDV4"/>
    <dbReference type="GeneID" id="59330110"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012923">
        <property type="entry name" value="Csm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040038">
        <property type="entry name" value="TIPIN/Csm3/Swi3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13220">
        <property type="entry name" value="TIMELESS INTERACTING-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13220:SF11">
        <property type="entry name" value="TIMELESS-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07962">
        <property type="entry name" value="Swi3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0236">DNA replication inhibitor</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome segregation in meiosis protein 3" evidence="9">
        <location>
            <begin position="70"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="150"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="266" mass="29970" checksum="F022E027F7F7920D" modified="2022-01-19" version="1">MATTDVAARNVPPVDDLDDSLFDYDVGDVFRDVDTNMDVPAARADEKENGAGLGIDEEIKVTKKRAPVPKLDENRLLSPAGIPKLRRIAKERLKFKGKGHEYSDMARLLNMYQLWLDELYPRAKFADGLAIIEKLGHTKRMQTMRREWINEGKPQDSLDSLGEGTEELAGEQRSSKAGQQQYWEVKSRERPRTPVAVVGDDNDIHAATPTFPRDDGKTERDGDTENLFLEDSTKTVPILGQESRSPTREENFADDEDALLALNDVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE94</accession>
    <name>A0A8H6CE94_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_001639</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151106.1">
        <property type="nucleotide sequence ID" value="XM_037292567.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59330053"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050265">
        <property type="term" value="F:RNA uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010605">
        <property type="term" value="P:negative regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF40">
        <property type="entry name" value="POLY(A) RNA POLYMERASE GLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="12">
        <location>
            <begin position="286"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="11">
        <location>
            <begin position="753"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="99"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="417"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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            <end position="526"/>
        </location>
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        <location>
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            <end position="593"/>
        </location>
    </feature>
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        <location>
            <begin position="886"/>
            <end position="948"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="114"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="192"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="220"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="253"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="486"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="928"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6221671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="948" mass="105331" checksum="840BACEAB005E380" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEU5</accession>
    <name>A0A8H6CEU5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151505.1">
        <property type="nucleotide sequence ID" value="XM_037292086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEU5"/>
    <dbReference type="GeneID" id="59329572"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="403" mass="44579" checksum="FC4544904DCECA38" modified="2022-01-19" version="1">MVCYPGKDPPLLAIVAQRFGGIVHENESSGEKPSSLRTLPSLRRGREAEERARRSSGAQYTTQVPLIQSVPKTWPQGSIPPIPASTSMNVNTFSRPPVQPLIRSEQGKTVSWSTKAPFTMSIDSGRKQIRFSHCPALTFPSMQYAYDYFHVQEKLHNQCAIQNGRPQRLQAAQRPLRAEQQGYLAEHLGLRAGQLANFAAQQACLAESLCPFEPVWQFDTPLKPIDAQDVLNLPTPPSHSVFDSQHQLATEPFAQSLWKESVPVHSSSGSPPPESPPQTLFAESTPHAREIQARPRLRLPVKGLQSWPFRSQVTNDNPFLQSSSIQPADQAMTLPGSYHQPSTCIGTVLPRTLTNEVDISDQPPTFHGLYHSPFSYADTVLPQTLNRKDRRVLGVSSSAGVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFN3</accession>
    <name>A0A8H6CFN3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000679</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151978.1">
        <property type="nucleotide sequence ID" value="XM_037291616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFN3"/>
    <dbReference type="GeneID" id="59329097"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000251">
        <property type="entry name" value="Ubiquitin ligase complex F-box protein GRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382:SF67">
        <property type="entry name" value="SCF E3 UBIQUITIN LIGASE COMPLEX F-BOX PROTEIN POF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="70"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="6">
        <location>
            <begin position="166"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="6">
        <location>
            <begin position="238"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="573"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="580"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="68100" checksum="FB50F0665F426BD4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGB6</accession>
    <name>A0A8H6CGB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152196.1">
        <property type="nucleotide sequence ID" value="XM_037291834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGB6"/>
    <dbReference type="GeneID" id="59329319"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010833">
        <property type="term" value="P:telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052833">
        <property type="entry name" value="Telomeric_DNA-bd_trans-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807:SF1">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="858"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="668"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="868"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="878" mass="96801" checksum="A73A00E783BC586C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHD5</accession>
    <name>A0A8H6CHD5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152471.1">
        <property type="nucleotide sequence ID" value="XM_037291036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHD5"/>
    <dbReference type="GeneID" id="59328515"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="277" mass="31405" checksum="13D47B204D67095F" modified="2022-01-19" version="1">MAFPISPQTILEFKLFKEKLHTLLDNVLDQAETLQTTAEEPVVLDRDWIIGKLKDQITTEKYVLEPGAVRVTEGLGNLLKLPRELRDLIYGYAIVDGQTAIVRASKQTHKEASELIFHHGVYRLVIGFGDDVINPPLSQSLVKNVKNLNLRVNGRGFFIDGVDQNLPILRMFDGSNIQRKDCVVTVVCDPFCGGMDASQVVRALDNLTGFERVILELDLEWWGEPWPDTIDETQEYRIWARINGAVHHQKKLLEPTLGTGSYSFDEKGLRLAFHPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHR1</accession>
    <name>A0A8H6CHR1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152929.1">
        <property type="nucleotide sequence ID" value="XM_037291494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHR1"/>
    <dbReference type="GeneID" id="59328974"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="292"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="413" mass="45818" checksum="A82DC7C3B725D190" modified="2022-01-19" version="1">MSLKGEAKAAEDGIAGYRQLLSRYKREVQSCNAKILEVEKALQTGTGLVPLAVLLRDREKTIAELTPHLTIRVEEVAHLHQMESKYENHVVEMGTDLQCMYDANDSLEQEVRKMKKEVKRVIRANDSLQQKVTLFESKTLLIEGHGGNKVDGGGANGRSHENPGASSEHERLDVENMPGDSCKDNNGGSQNYDDASMTTDSNDVISRQTSTSKSGSGKHSADVESSYAADIEKEPNQVSRVDRFEAEPFKILKPAGPTNPSIAGKRTQVSQPTSTGIFSFERKSPPLVTIQPKEKTAEDGVPKDENPEENMPKEKVPEEGAPREETREIDAPEDESPREKNLREKKLKKKILKEGSARKEETPKEAPAASEPSRSQKRAVKKQQQKGVKKAEREAHSANAQVSRRVQQAMMMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIC5</accession>
    <name>A0A8H6CIC5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mRNA stability protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010616</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an essential role in initiation of the G0 program by preventing the degradation of specific nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153102.1">
        <property type="nucleotide sequence ID" value="XM_037301468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIC5"/>
    <dbReference type="GeneID" id="59339006"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF6">
        <property type="entry name" value="ENDOSULFINE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="54"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="152" mass="16096" checksum="9FAB809A20A80A49" modified="2022-01-19" version="1">MNPHQKNKVDISSLNEEEQKLFRLYGKLPNKKDLLQNKLKERKYFDSGDYALSKAGKASEGGVTSIGSQHPLPENIPHLTSPGPNSGSNGNSNGASNGSIPGASSGSPVKESSFLQRETSVDEGSVLSPKSPERGSASSPPSKEGVPIRSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJG2</accession>
    <name>A0A8H6CJG2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153379.1">
        <property type="nucleotide sequence ID" value="XM_037301745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJG2"/>
    <dbReference type="GeneID" id="59339286"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="330" mass="35743" checksum="E1DB0AC29B2744C8" modified="2022-01-19" version="1">MAAPPTLPALLTTLTDSLASAAPSLPSTTNLAPPAEGISLLDTKNELLLSYLHNLVFLIILKLRNRSSPNAADESSLHDAVTHKLVELRIYIDKGVRPLESRLKYQLDKLLLAASEASTAPVANSAVETSRTAERRGAESDASASDSDAESAPRPAMPDLAHRPNPSAFARPSGTKAGRASASDGLYKPPRITPTALPTTDASSRKKEVKPRKSHTLDAFIREEMDDAPVAEPSIGTGSGLRGREKERDEERRGYEETRLVRLPGDKKKKDRRKGEGFGEDLAGGLGDFEFEGLKGRGGKRKRERDGGDEGRVGQAWEKRVKKGVGRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJG7</accession>
    <name>A0A8H6CJG7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153326.1">
        <property type="nucleotide sequence ID" value="XM_037301692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJG7"/>
    <dbReference type="GeneID" id="59339231"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="165" mass="18821" checksum="870B3334C4CB6A4F" modified="2022-01-19" version="1">MPSGDRLDTVRIPHDGSSCKEMSMPLASPGEGGISLEKCNNVEKQLLCFPQAWACEAKDAQRFSWEHRSLIRIKAESLSEFSPEWRADYFMYTCLDKTSGLPLNRYLDEVSDTKVYGDAIVFQMYKGQYRHLGRDFILAAEDEEYPRVVLQAILSQKNILTSQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJN6</accession>
    <name>A0A8H6CJN6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Iron-sulfur cluster biogenesis chaperone, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010044</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the assembly of iron-sulfur (Fe/S) clusters in mitochondria. Assisted by the DnaJ-like co-chaperone jac1 and the nucleotide exchange factor mge1, it mediates ATP-dependent Fe-S cluster transfer from the scaffold proteins isu1/isu2 to grx5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153717.1">
        <property type="nucleotide sequence ID" value="XM_037300900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJN6"/>
    <dbReference type="GeneID" id="59338436"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11733">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000020">
        <property type="entry name" value="Chaperone protein HscA homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000007">
        <property type="entry name" value="Heat shock protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0067">ATP-binding</keyword>
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            <begin position="706"/>
            <end position="773"/>
        </location>
    </feature>
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        <location>
            <begin position="736"/>
            <end position="745"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="773" mass="84160" checksum="0651CF32BBCD6E42" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKC9</accession>
    <name>A0A8H6CKC9_9LECA</name>
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        <recommendedName>
            <fullName evidence="4">Glycosyltransferase family 69 protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">HO133_010114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6224920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153787.1">
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    <dbReference type="GeneID" id="59338506"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR021047">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CAP59_mtransfer"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6224920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="54645" checksum="56D54DB47AD1C636" modified="2022-01-19" version="1">MPQRPVYSDEYELAGRDSYDSDDIYDEADFQSQLFFDLDEADFQSRGQTSPSYINRPTCYAFVGILLALVLFTALFSEPSYTKPPAHYQSLKARIEGSEDYGRANPENQKIFIAASIYDEGGHLLGGAWGEAVLKLINILGNRNVFLSIYENTSGEGGQVAQFKFERRVQCQHSMVLEDSPPLEELQRIRLPDGSERTKRIAYLAEVRNRALLPLVEHPEIKYDKLLYLNDVAFDPIAAAQLLLSTNMDEHGNTAYRAACAVDFINPFKFYDTFATRDLEGYSMGVPFFPWFTNAGSGSSRRDVLAGKDAVRVKSCWGGMVAFDAKPFQANPPLRFRATPDTYWDASECCLIHADLLDQSTFGADGDYVGVYMNPFIRVAYSNTTLPWIRKVQRVERLFTIPHNVINHLVGLPWFNPRRTEVAGSQVEEKVWVEDKSLKAGGSFQKESREATRGGYCGMRTLQLIKESQRDGEKNWETMPVPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLI6</accession>
    <name>A0A8H6CLI6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009597</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
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            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
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    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6225597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:tubulin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053005">
        <property type="entry name" value="Nuclear_Pos-Cytoskel_Interact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024774">
        <property type="entry name" value="PH_dom-Mcp5-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190:SF1">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12814">
        <property type="entry name" value="Mcp5_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1249"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="360"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="1037"/>
        </location>
    </feature>
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        <location>
            <begin position="1096"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1382"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1503"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1575"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="425"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="180"/>
        </location>
    </feature>
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        <location>
            <begin position="377"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="492"/>
            <end position="507"/>
        </location>
    </feature>
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        <location>
            <begin position="637"/>
            <end position="649"/>
        </location>
    </feature>
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        <location>
            <begin position="654"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="703"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="783"/>
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        </location>
    </feature>
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        <location>
            <begin position="805"/>
            <end position="823"/>
        </location>
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        <location>
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        </location>
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        <location>
            <begin position="855"/>
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        </location>
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        <location>
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            <end position="971"/>
        </location>
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            <end position="1166"/>
        </location>
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        </location>
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            <begin position="1397"/>
            <end position="1407"/>
        </location>
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        <location>
            <begin position="1408"/>
            <end position="1422"/>
        </location>
    </feature>
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        <location>
            <begin position="1425"/>
            <end position="1439"/>
        </location>
    </feature>
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        <location>
            <begin position="1470"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1601"/>
            <end position="1612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1612" mass="175341" checksum="42CFFC0DD4294A20" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNA3</accession>
    <name>A0A8H6CNA3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154927.1">
        <property type="nucleotide sequence ID" value="XM_037300101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNA3"/>
    <dbReference type="GeneID" id="59337635"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="183" mass="20080" checksum="847A2E8F9ED3724F" modified="2022-01-19" version="1">MSIRPKDPPTRNAKKPFSRAVIPHIPLCSHIKGRSKRYESFYEVSGLFLQDPQEPGTSTSSGSPKTAIPLHLLKDGQSGERVAINKAASIKSAGRVAINKAASIKSAGRSTISKSPSVHSAADSQRRESALPPKKYEDVESVYDENERDDSGRPTGKRLKDAPRPGGITVRWVEEESLREWYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNP5</accession>
    <name>A0A8H6CNP5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ER-derived vesicles protein ERV14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008466</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155333.1">
        <property type="nucleotide sequence ID" value="XM_037299332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNP5"/>
    <dbReference type="GeneID" id="59336862"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="13078" checksum="4B176397CB12F98F" modified="2022-01-19" version="1">MYSDLECDYINPIDLCNRLNTYIVPEAAVQAFLTVLFLINGYWIALVLNLPLLAYNAKKIFENQHLLDATEIFRKLNVHKKESFIKLGFHLIMFFFYLYSMIVALIRDESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPD1</accession>
    <name>A0A8H6CPD1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diphthamide biosynthesis protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_008649</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155515.1">
        <property type="nucleotide sequence ID" value="XM_037299514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPD1"/>
    <dbReference type="GeneID" id="59337044"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF46">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="J" evidence="13">
        <location>
            <begin position="9"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="DPH-type MB" evidence="14">
        <location>
            <begin position="101"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6227207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="168" mass="18707" checksum="6D34B1A84CFDF48F" modified="2022-01-19" version="1">MPERSYTQNHYEVLGLPSNPSTSKDLTDQDIRHAYRRALLLHHPDKSRPSPASPSTSKYTIDAITLAYKTLTDPSARSEHDLSLITSSSATRVPVQPSHPGLETVDLGDMGYDEKEQMWYRGCRCGKDGAYVITEEELELNAEYGETITPCQGCSLWLRVTFAVAEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPH4</accession>
    <name>A0A8H6CPH4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pre-mRNA-processing factor 39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008627</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PRP39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155493.1">
        <property type="nucleotide sequence ID" value="XM_037299492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPH4"/>
    <dbReference type="GeneID" id="59337022"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000451">
        <property type="entry name" value="mRNA splicing protein (Prp39), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000064">
        <property type="entry name" value="Putative pre-mrna-processing factor 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF5">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="555"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="579"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="600" mass="70778" checksum="72AB76AF5E5C7344" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPJ7</accession>
    <name>A0A8H6CPJ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphomannomutase</fullName>
            <ecNumber evidence="5 13">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_008783</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155647.1">
        <property type="nucleotide sequence ID" value="XM_037299646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPJ7"/>
    <dbReference type="GeneID" id="59337178"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAD-like_sf"/>
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    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
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        <property type="entry name" value="PMM_cap"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF0">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
        <property type="entry name" value="C2.B.3:_Phosphomannomutase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6227339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="269" mass="30337" checksum="8FFF2877CE6942C0" modified="2022-01-19" version="1">MYAEAASVYPPLEGRPIKDTICLFDVDNTLTLARQSVSPEMLQVLSVLRHKCAIGFVGGSNLVKQQEQLGTPSIPVIGLFDFCFAENGLTAYRLGEQLASNSFIGWLGQEKYKQLVNFCLRYIADIECPVKTGTFVEFRSGMINVSPVGRNASIQERNDYEKYDKQHQIRAKFVEALKEKFGDFGLTYSIGGQISFDVFPTGWDKTYCLQHLETEKSLSGVDYSTIHFFGDKTYQGGNDYEIFEDSRTVGHSVDNPDHTMRVLKELFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPQ5</accession>
    <name>A0A8H6CPQ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155707.1">
        <property type="nucleotide sequence ID" value="XM_037299706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPQ5"/>
    <dbReference type="GeneID" id="59337238"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF64">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="326"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="847" mass="97665" checksum="CC313937902A5008" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ05</accession>
    <name>A0A8H6CQ05_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA2/NAM7 helicase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155731.1">
        <property type="nucleotide sequence ID" value="XM_037299730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ05"/>
    <dbReference type="GeneID" id="59337262"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF495">
        <property type="entry name" value="HELICASE SENATAXIN ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="157" mass="17354" checksum="C6DC34F1D6B9AE6E" modified="2022-01-19" version="1">MATTDSAQGKEYDFVILDLVSPGGRQYALGFRTDPRRMCVALSKAKIGLLIIGNKNKANVAYPGAGTRAWKQLIDDHVTKDAVCEKDAWDFSGQVKALMREHYIPGVLYEEAPRRGYLIARYPYSTIAKTLANILLEGADISSILLSMDYGAIFSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQF9</accession>
    <name>A0A8H6CQF9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sphingolipid C(9)-methyltransferase</fullName>
            <ecNumber evidence="14">2.1.1.317</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_008815</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CFA/CMAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.317" evidence="14"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155679.1">
        <property type="nucleotide sequence ID" value="XM_037299678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQF9"/>
    <dbReference type="GeneID" id="59337210"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052290">
        <property type="entry name" value="Sphingo_C9-MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197:SF1">
        <property type="entry name" value="SPHINGOLIPID C9-METHYLTRANSFERASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197">
        <property type="entry name" value="SYNTHASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G04190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6227371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="527" mass="59710" checksum="2C4AFB35050E7606" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQL5</accession>
    <name>A0A8H6CQL5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007592</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155798.1">
        <property type="nucleotide sequence ID" value="XM_037298462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQL5"/>
    <dbReference type="GeneID" id="59335989"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="243"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6227864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="460" mass="52456" checksum="9DCB45888A78C117" modified="2022-01-19" version="1">MSSISLTHLPLGNVPKLVSTDPPSMAAWDSINAWYQKVVDNKGEKKILLTAASFIMMACRFINLRVQKMTMDESAPRLAAGLVIKKMQEVSGEFKKMQAVTDWLGQRSSLKASFDVGTVMYNTVYQQKHHYGATQPLSKGILLPIHRVWTLISLMIEIAPEPLIVKSRDVKDKLAWLQAQLDESSKRLGQDNEDDKKSKKVKTAIGEVLKRIEVIHPDDLEIIKRYDNNIGVVPTEGSVKNRSVVTAYDVSTLSQGQWVNDVAIDAHLTLVCHTFNGLFQGGDELPKSPKYHAWSVTMSVYLKGRVEAFEEKHLRREWPPARFPDAALEDVSCHIFPIHVRKNHWILMVLQKMGDGQWSLFHYSSLAGYDKDFEEPWQVISSWLLFKSKGTLNVTRTRVIVPKPQPTQDNDRDCGVFVCGIVRWSLADWDLSTLTPSIIPEYRRRMMLELENWRLSTNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR52</accession>
    <name>A0A8H6CR52_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007879</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156083.1">
        <property type="nucleotide sequence ID" value="XM_037298747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR52"/>
    <dbReference type="GeneID" id="59336276"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF30">
        <property type="entry name" value="TR-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="2">
        <location>
            <begin position="264"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="865" mass="92321" checksum="D1E99276A99FD036" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRZ9</accession>
    <name>A0A8H6CRZ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_007881</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156085.1">
        <property type="nucleotide sequence ID" value="XM_037298749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRZ9"/>
    <dbReference type="GeneID" id="59336278"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF14">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="465"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="56"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6228151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="605" mass="67060" checksum="A5ED4C0D7771D7BE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7W7</accession>
    <name>A0A8H6F7W7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RTA1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005829</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147915.1">
        <property type="nucleotide sequence ID" value="XM_037296734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7W7"/>
    <dbReference type="GeneID" id="59334234"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF11">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10770)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="32416" checksum="9C22CE3D9C8BB1F8" modified="2022-01-19" version="1">MALLDRSSTLAATLDNSQRGKGCVPYDTNATAPTYGYDPDLGAGIVFTVVFFLSMLAHIIQVTMSRKWWYSAFALGAFGECLGWAGRAGAHNCPYNKTLFSLQISILIISPCFFSAAIYYILGQMITQYGRRYSLISARTYLAIFVSFDIISIVIQAVGGASASKAGGEDPPGNTAPGTHIMMAGIIIQLVSMSAFGLLWLIFLWRARAVPISRVLLVVTTFSAFCIIVRNFYRAIELSQGWKGYLITHEVYFAVLDGALMALSVGVFNIFFPARYLTGEAELDIRDHEMLTTEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F858</accession>
    <name>A0A8H6F858_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Concanavalin A-like lectin/glucanase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006170</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147644.1">
        <property type="nucleotide sequence ID" value="XM_037297070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F858"/>
    <dbReference type="GeneID" id="59334572"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11618">
        <property type="entry name" value="ChtBD1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00413">
        <property type="entry name" value="Glyco_hydrolase_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38121:SF2">
        <property type="entry name" value="ACYLTRANSFERASE 3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38121">
        <property type="entry name" value="GH16 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034142039" description="Concanavalin A-like lectin/glucanase" evidence="4">
        <location>
            <begin position="17"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="5">
        <location>
            <begin position="35"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="6">
        <location>
            <begin position="122"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="36723" checksum="C4B78034905764C8" modified="2022-01-19" version="1">MYMSIDFLLLISAVSAIPYPNHDDASKHKRTLTPDNTCGGSNGYTCNPNDSYGGACCSAAGYCGNTDAYCGSGCQSTFGTCGSPTSPQPQASSLPQVSPLPPPTGPAGSSSPATKEGCLWTIDGSGSFTHILTVDFSNLTALPTDTLDVSTDHIPSIPYTQTYTIENVAVEDGTLQLKVPGGQTASPILGAEISTAEKNILYGSVRTMMQISSVSGTVSGAFFYKSDSQEADIEVITGGAYEGVHYTNQEASPTADQTTVALASPVDLTSEMHEYRLDWLPDRTDFYLDGVKQADLTGNVPSTAGAWLWNNWSNGDPKWSAGPPSMDNILKISKIEMYYNTTTSTGAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F898</accession>
    <name>A0A8H6F898_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005660</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147749.1">
        <property type="nucleotide sequence ID" value="XM_037296568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F898"/>
    <dbReference type="GeneID" id="59334065"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034536382" evidence="3">
        <location>
            <begin position="35"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="224" mass="24267" checksum="EFAD575CD1F1AFDE" modified="2022-01-19" version="1">MASTRPTTSIFSFSSKLLSSLLILLATQPCSLNAQPQQSPTSTTSSSAIPSLVNNPANGDTAPSSFTPSSLSSAIPSATDSAGYGSQDNNTTSSPHNGILNYYFLLLAVFVVAILVIYWSLARRRRKAAARLSRMQQRVLSEDVRSWTRHRQPGQGLEDEERGGTGRDEGLDEAGEAPPAYVKEPERAHLDRGEGVELRGMARFREMVGLEGKPPDYEEGLPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8A8</accession>
    <name>A0A8H6F8A8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005443</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148335.1">
        <property type="nucleotide sequence ID" value="XM_037296353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8A8"/>
    <dbReference type="GeneID" id="59333849"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07730">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978:SF5">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="55"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="363" mass="39488" checksum="A63769B42D105FD0" modified="2022-01-19" version="1">MDIWPIAPKPPPALHIPESTSAVDVRVIDTNTLVHLNPELFWQPKVDGFAGLDAPIYCFLISNGSQHVVFDLGVRSDWQNYAPKVVSLIKATTVITRGSDVTSVLDTDDSGLNIHSADIQAVIWSHNHFDHIGDVSKFPPSTDLVVGPGVCAASWPGWPRRPDAIVLDSDAEGRVVREISFEASSLKIGSFDAYDFFGDGSFYLLEAPGHAVGHLCGLARTTAHDDEPSFVFMGADACHHPGVLRPSDYLSLPSADTLQSTLATTRSSPEQCPGALFQSHLTSPNAPFFTIAPGPLFPDHDAAMLTVRKIQELDAADNVLVLMAHDLSLRDKIPLFPETINGWRVSGLKTKTRWLFCNDFLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F936</accession>
    <name>A0A8H6F936_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149160.1">
        <property type="nucleotide sequence ID" value="XM_037294472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F936"/>
    <dbReference type="GeneID" id="59331961"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="49"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="30354" checksum="760E91331BC4247D" modified="2022-01-19" version="1">MSVTDVEGWGAAAAAYAQVERVTTPPSQSLLNRVSAILPLNAPNTTAFDNGCGTGVLTTILKQQNPNVPLLAADYSDGMVDILKRRVQAQKLEEVTARVVDGRNLQGIKDGSFTHTFSTFMVCLAPEPDKIVAEMHRVTKQGGVLGLAVWGDPRFGPFSSPWEKACRRLISDYEPPMIMPAEWTLAENVKAGLEKAGFGDVDVWVENVAWRWDSVEALSGYYFEGGNPGNVKVLDSFKALGGKVDEARPIFERIIKEECGKEDGSVELRVPATLATARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9N4</accession>
    <name>A0A8H6F9N4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pirin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003232</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149536.1">
        <property type="nucleotide sequence ID" value="XM_037294155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9N4"/>
    <dbReference type="GeneID" id="59331643"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="3">
        <location>
            <begin position="100"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="4">
        <location>
            <begin position="255"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="41462" checksum="1CEBE4B29C978C01" modified="2022-01-19" version="1">MKLLISYSIISAVAILFYSSSFWSQLTSLLHQTPLASFLSTTQNPSNGPSRRASSNSTSFFGLPLVPPTFFAPNKPTNMTVPRAIRQAFLAIEQSEGAGARVRRSIGTPKLRNFSPFLMLDHFSVAPGAGFPDHPHRGQETITYLLNGAVDHEDFAGNAGTIEAGDLQFMTAGRGIMHAEMPRQNPDGSANVGMQLWVDLPEKLKTCEPRYRDLRAAEIPTIKVDDGNVTIKIISGQSHGTDSVQELAYTPVWIFDIKIAPGGKVTQPLPVGWNAFAYTLAGSTAFGVGKDQTTVGQYHNVVFEQKGDSVSAEVAKEANENGQFILVAGLPLDQKVVQYGPFVLNTQEEVYQAMLDYQSHQNGFERAKGWQSEIGKSMVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9T7</accession>
    <name>A0A8H6F9T7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149925.1">
        <property type="nucleotide sequence ID" value="XM_037293847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9T7"/>
    <dbReference type="GeneID" id="59331335"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="344" mass="38860" checksum="E14621F2D9FBD130" modified="2022-01-19" version="1">MPAEPPTPIETSIPNRSYLLKLDCISCPFLIRPSNRNQWEKDPRSNQLTLALYIDGPHPHLFLQGETLNPLYYERRAPSFYSQPHPFAPPFTANQIGVNETLEQEENNNGWGIPVRQFELAYEFDVVTYAPKDKVREWEVLIEFQITGLRHEEHVPKDIPFSGGVSSPETEGVVPGVTSVVRLVLGYTAFEEIRIDALELMPKETMAKVAGKQIEVLRKAAESKGEKSITTANWGGRLRDINGPDGKDIYTEHASFKPGEWNDCGRKDEWGRQIMCKVDDFKAWSPMIFVISLMLLAVVLIGIFVLVVFMAWLAIRFLGGQKIQEVVTAKGENDALLLFGDEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9Z4</accession>
    <name>A0A8H6F9Z4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002442</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150197.1">
        <property type="nucleotide sequence ID" value="XM_037293368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9Z4"/>
    <dbReference type="GeneID" id="59330855"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="142" mass="15101" checksum="6ED558C0862D0F65" modified="2022-01-19" version="1">MSGAVFGLSNNFWVLLAAATVGVISPSLIGMIGQALGTIVSGSVIEALESRDGWTTARAYHIIFFGYGVLGIVKTVLCLMLSQACEIDIKSVNLAHRENASVPPSENLRLLPDDGKKDKGRRGWQVASSTSKESLAALSSWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA39</accession>
    <name>A0A8H6FA39_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149728.1">
        <property type="nucleotide sequence ID" value="XM_037294347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA39"/>
    <dbReference type="GeneID" id="59331836"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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</entry>
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        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
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            <dbReference type="PubMed" id="32504651"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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            <dbReference type="PubMed" id="32504651"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
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    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="463"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="43"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="279"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="417"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="499"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="303"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="523" mass="56372" checksum="A87B173A04FD34D4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAV7</accession>
    <name>A0A8H6FAV7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein transport protein sec13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_002710</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. It also functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. SEC13 is required for efficient mRNA export from the nucleus to the cytoplasm and for correct nuclear pore biogenesis and distribution.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The COPII coat is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex, and the protein SAR1. Component of the nuclear pore complex (NPC). NPC constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SEC13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150464.1">
        <property type="nucleotide sequence ID" value="XM_037293635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAV7"/>
    <dbReference type="GeneID" id="59331122"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090114">
        <property type="term" value="P:COPII-coated vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032527">
        <property type="term" value="P:protein exit from endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000017">
        <property type="entry name" value="SEC13 homolog (S. cerevisiae)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF2">
        <property type="entry name" value="PROTEIN SEC13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0811">Translocation</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="212"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6221029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="307" mass="33679" checksum="3DA89B4EF2030453" modified="2022-01-19" version="1">MSQAQLITNSGHDDMIHDAVLDYYGRRLATCSSDKTIKIFEVEGENHKLTETLRGHEGAVWCVSWAHPKYGSILASSSYDGKVLIWRESPNSQWTIVANFALHTASVNIVSWSPHESGCLLACASSDGQVSVLEFQEGNWTHQIFAAHGQGVNAVSWAPATQPGSLVSSKPGVDGKGAGGGVRRFVTGGSDGVVKIWEYSTQDKTYLPIKELAGHTDWVRDVAWAPTILQKSYIASAAQDKTVRIWTSDPAHPNDWKPTVLNFDAVLWRVSWSLSGNVLAVSGGDNKVSLWKENLKGEWDCVKWIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAZ7</accession>
    <name>A0A8H6FAZ7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150673.1">
        <property type="nucleotide sequence ID" value="XM_037293020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAZ7"/>
    <dbReference type="GeneID" id="59330507"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="142" mass="14941" checksum="93D0B00CA871115A" modified="2022-01-19" version="1">MSGIGYSGSGSGSGSQGHLGAPAFVSSSSTGNIRELWIDCGTDGWKTLRACILISLPVFDLDPVRMLPMDDLVAPVLGDALPSGSGEEDGFLKIQLNRGRYVMADAPSMAAETSTTDQREGSTSAQVGSYEYRDNPRSCGMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBR0</accession>
    <name>A0A8H6FBR0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the avfA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151726.1">
        <property type="nucleotide sequence ID" value="XM_037292307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBR0"/>
    <dbReference type="GeneID" id="59329793"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051606">
        <property type="entry name" value="Polyketide_Oxido-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43355">
        <property type="entry name" value="FLAVIN REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43355:SF2">
        <property type="entry name" value="FLAVIN REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="7"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="180" mass="19919" checksum="66F03AFEA1665167" modified="2022-01-19" version="1">MKILIIGAAGRVGSAALAACLTKQHHVTAFLRNPSKLPPELHNHPRLRITQGDATSHSSLVEAIEDTDAIIQAAVYGSNTPFGTSDSEKVVRCIINAVKEVQASRPIRSRPIRLWVMSGQVLMDIPGYAGKIEGDVFPIHPEHYKNYDFLRNDAKDVDWSLLCPGRIDQGEVRVLLYNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBR9</accession>
    <name>A0A8H6FBR9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial import receptor subunit tom22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_001407</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151756.1">
        <property type="nucleotide sequence ID" value="XM_037292337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBR9"/>
    <dbReference type="GeneID" id="59329823"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22884">
        <property type="entry name" value="TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005683">
        <property type="entry name" value="Tom22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04281">
        <property type="entry name" value="Tom22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6222321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="15754" checksum="CD28801403DC9613" modified="2022-01-19" version="1">MVQLEEVEDAELDQAQPGPVGDDYDSDDFVDTDSELSDDEIISSGPPAESITDRLLALRDILPPRYRRALSSTLSSTLSATKTGAWWGGKGLWVLTTGAVMLSVPFAVCMVEEQQIMEQEREMKAQEGAREVLVPGVGAGPGAAGSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBW7</accession>
    <name>A0A8H6FBW7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000742</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152041.1">
        <property type="nucleotide sequence ID" value="XM_037291679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBW7"/>
    <dbReference type="GeneID" id="59329160"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14521">
        <property type="entry name" value="DSP_fungal_SDP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="388"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="457"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="266"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="555"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="742"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="833"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="877"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="151"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="564"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="606"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="642"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="673"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="747"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="833"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="925" mass="99072" checksum="2658E0EBCD9D2F99" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBZ8</accession>
    <name>A0A8H6FBZ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152146.1">
        <property type="nucleotide sequence ID" value="XM_037291784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBZ8"/>
    <dbReference type="GeneID" id="59329267"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1240">
        <property type="entry name" value="DOPA-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023389">
        <property type="entry name" value="DOPA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014980">
        <property type="entry name" value="DOPA_dioxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36423">
        <property type="entry name" value="AFR070WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36423:SF2">
        <property type="entry name" value="AFR070WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08883">
        <property type="entry name" value="DOPA_dioxygen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143410">
        <property type="entry name" value="DOPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="185" mass="21241" checksum="A50327C255779C97" modified="2022-01-19" version="1">MTASSEYVYPSPLKGYEHLPPLSDEKNDDNKSFVNPKTNDLSKAYEEFVNPIGRGFDVHIYFFQENKAQTTFAHELWTRIRHEFPELRIYRVWDRPLGPHPVGMFEVNVVTPAQFGAFVSWLVINRGPLSALVHPNTGDDERDHTQRATWLGDRLPLDVSLFKQMREASMKSENVGGGQIEEKHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCE1</accession>
    <name>A0A8H6FCE1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001002</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152297.1">
        <property type="nucleotide sequence ID" value="XM_037291935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCE1"/>
    <dbReference type="GeneID" id="59329420"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="610"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="688" mass="79450" checksum="02EFF84C9AD74FA8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCQ2</accession>
    <name>A0A8H6FCQ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152453.1">
        <property type="nucleotide sequence ID" value="XM_037291018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCQ2"/>
    <dbReference type="GeneID" id="59328497"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="134" mass="14869" checksum="AD486CE2954BFF76" modified="2022-01-19" version="1">MATCLTTIAYRIMMERLIKQLPPLWPQNGPTNPQSHCCDPWLVEDNLKTLAILDKLTGRGGGKDEVLPTMDPASMRHVDPHTFLKSHSLEHMRANASPQAEELQQEMNKLSERLEDKVGAIGVPEHPALPTAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD94</accession>
    <name>A0A8H6FD94_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000451</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152825.1">
        <property type="nucleotide sequence ID" value="XM_037291390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD94"/>
    <dbReference type="GeneID" id="59328870"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="142" mass="14756" checksum="93548B444389826A" modified="2022-01-19" version="1">MLPPSTPIDYFLYISLLISTTFAQGRIAQFLVLDCTEKSAVNPTVSLPLDTCLVTTGAFGVVVERLPACPPGSANATLQLYQDQSCAIPETSSDFDTNGCFDYGLVGIPAVMFICGSVADGDSDATSTTTCAAGVAMPEAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDH4</accession>
    <name>A0A8H6FDH4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010907</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153390.1">
        <property type="nucleotide sequence ID" value="XM_037301756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDH4"/>
    <dbReference type="GeneID" id="59339297"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="86"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="33"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="289"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="354" mass="38889" checksum="2A4E269C28B1AF40" modified="2022-01-19" version="1">MSSMNDSMQDTKGSLYDNAQSAANTIQNHPATQSVKDAMGNGPVADHVKDQHAKTTSEFRSLADSRTTPEQSTANGQPLTHYHSFFYNLLSWENPRATTLSFVSIVLSIFAARYLPALRWSFKALWMVLGITASAEIAGKLVLSQGLASSFRPKKYYTIPREALEASLEDIEQFINFFVIEFQRVLFAENVVHTVAGFVAALLSYWLIKVVPFWGLSLIAVSCIYMGPLIYMTNKELIDHHVKNASEVANAQANQLKDLAGHHTARAHETVKSYAGDYSAKAQEYMGNARGRSTSPEVNSSKPTSDAPVKSEPGSDLSASDFPHAPKQEPTSGVTSHQEQYKNSQFGGQAEPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDM3</accession>
    <name>A0A8H6FDM3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_011057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153540.1">
        <property type="nucleotide sequence ID" value="XM_037301906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDM3"/>
    <dbReference type="GeneID" id="59339447"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF37">
        <property type="entry name" value="CLAVALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="293" mass="31827" checksum="09B7C0B580A24495" modified="2022-01-19" version="1">MPPNPAAAGYNFTSTLRNDTYPAIDPTKLKLGGKYVFLSGASKGIGHAAAISFVKAGVAGIAIAARSDLSSLEREMHEAATAAGKPSPQIKSIRLDILDAQSIERAAQETSDTFGKLDILINNAGAMEEFKPTLDSDPEQWWQTWTVNIRGTYLLTRALLPLMLKGGDKQIIMLTSIGAFMPAPGGSGYQISKLALLRYAEYLNIEYGAQGVVAYGIHPGAIPTDIGLTMPSFLHHKLIDKPALAADTIVFLVSERREWLRGRYVSCTWDMLEFGEMEEEIVGGDKLKIRLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDR3</accession>
    <name>A0A8H6FDR3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">STF2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153179.1">
        <property type="nucleotide sequence ID" value="XM_037301545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDR3"/>
    <dbReference type="GeneID" id="59339083"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="17766" checksum="936E3A2CC19AD768" modified="2022-01-19" version="1">MCPVEERWSVSQLIISIHIATMTRSHKQNDRDHAGLADGTAEESQHLPRYFAKSGHVDADPKKVKKEGGGKGNWGTPGDESQDYGYNFTNARRRSNSSSHGHAIGDFKTKFETVEADPVFEEEYHGAKDDAGVDEHLGLEKGDSADSISGGSIEEEEHAKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE04</accession>
    <name>A0A8H6FE04_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoglycerate mutase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010981</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153464.1">
        <property type="nucleotide sequence ID" value="XM_037301830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE04"/>
    <dbReference type="GeneID" id="59339371"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052765">
        <property type="entry name" value="PGM-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46192">
        <property type="entry name" value="BROAD-RANGE ACID PHOSPHATASE DET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="278"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="50901" checksum="3C500F1ADDC191FE" modified="2022-01-19" version="1">MGKPRMIILIRHAQSEGNKNRDIHQTVPDHRVKLTQDGWKQADEAGQKLRSLLRPSDTLHFFTSPYRRTRETTEGILNALTSPAPDHKPSPFPRHTIKVYEEPRLREQDFGNFQPDSAEMSRMWQERADYGHFFYRIPNGESAADAYDRVSGFNESLWRSFGESDFASVCVLVTHGLMTRVFLMKWYHFSVEYFEDLRNMNHCEFVVMKLDEDNKKYILQNQLRTWSELKRERANSRTSITEPESPIPVRKKWGGCLQTDGDRGDDFARRQMARRRKDTKELFEDVEMEHGNKELGNGKKADGSESEYSRGREDRTQAPERENSQTDAANVLLATPGARTRIVSPNRLEILKGGRDGGGSRSGAASPSLSSLDDSETEAKERMRSLPLRRSLAMALNGELDGDGERVRADALGDQSDVEDEEEEEHRKEVAKMEAKDKSFEGAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEM8</accession>
    <name>A0A8H6FEM8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nucleoside transporter family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010458</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154128.1">
        <property type="nucleotide sequence ID" value="XM_037301311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEM8"/>
    <dbReference type="GeneID" id="59338849"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034257">
        <property type="term" value="F:nicotinamide riboside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF88">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="48581" checksum="1B02A3D9CF0EF241" modified="2022-01-19" version="1">MERIRNALKPRRSPAYEPLDDDSDSNEPRRARKPRFSWLEYGIFLLLGVSMLWAWNMFLAASPYFQHRFRSSHALLSTFSPALISVSTVTNLVSMLLLTKLQKGANYPNRIILSLILNIVSFTLLALSTVLFRGVSPGAYFGFLMAIVFTASLATGFCQNGVFAYVAGFGMAEYTQAIMTGQAVAGVLPCIAQIVSVLSVSAPTYDDGRDGATVPQESGKSAFAYFLTATAVSVAALLAFMLLLRRHPDDAMDASTPDRGAEAPEHASRKVVGLWTLFQKLRYLSSAVFLTFAITMFFPVFTQQIVSVRPADTAPRILQPACFIPLAFLFWNTGDLLGRLITAIPRLNLVLWPRTLLLMSVARFAFIPLYLLCNIQGGGAALASDAFYLVVVQFLFGLSNGYLGSTCMMGAGEWVDVDEREAAGGFMGLMLVGGLTVGSLLSFLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFB1</accession>
    <name>A0A8H6FFB1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sphingomyelin phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009253</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Converts sphingomyelin to ceramide.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acid sphingomyelinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154940.1">
        <property type="nucleotide sequence ID" value="XM_037300114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFB1"/>
    <dbReference type="GeneID" id="59337648"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006685">
        <property type="term" value="P:sphingomyelin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011160">
        <property type="entry name" value="Sphingomy_PDE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF27">
        <property type="entry name" value="ACL091CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000948">
        <property type="entry name" value="Sphingomy_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034661419" description="Sphingomyelin phosphodiesterase" evidence="6">
        <location>
            <begin position="22"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="7">
        <location>
            <begin position="234"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="122"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="255"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="261"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000948-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000948-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="687" mass="74665" checksum="FFE0C715DCA7F977" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFM3</accession>
    <name>A0A8H6FFM3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008949</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154637.1">
        <property type="nucleotide sequence ID" value="XM_037299811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFM3"/>
    <dbReference type="GeneID" id="59337344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="109" mass="12044" checksum="411CC0F096F85747" modified="2022-01-19" version="1">MEISHDMDASTTKGEVYTENQGDIEAEAKNKEETITNNKPITKDEADTKGESPKANISQNEATEDDAPADDNDASDRQHTDTKPMSPRPKHGRMVQNHLDEASPVSTEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFU6</accession>
    <name>A0A8H6FFU6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154862.1">
        <property type="nucleotide sequence ID" value="XM_037300036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFU6"/>
    <dbReference type="GeneID" id="59337570"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16992">
        <property type="entry name" value="ENTH_Ent3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF45">
        <property type="entry name" value="CLATHRIN INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="27"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="424"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="450"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="542" mass="57406" checksum="8B99DFCECA0FFA4E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFW0</accession>
    <name>A0A8H6FFW0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008458</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155325.1">
        <property type="nucleotide sequence ID" value="XM_037299324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFW0"/>
    <dbReference type="GeneID" id="59336854"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="16"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="151"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="289" mass="32665" checksum="90BEB376F9F4D0E1" modified="2022-01-19" version="1">MAWDGTSRHSGQFDPLNNRDLELTIEKDVYVSEIHYPITITAIKISILLFYLRLFGARKWFRYVIYITEAIVLSWCMATILPAVFQCKPIHIAWSIDAIEMMEHCNNLNAYLIATSVINVILDFWILALPLSIVWTLQMSRKSKASLSGSMVDPIIWSNVEISVGIICACLPVLRPLVQAVGRRLFGGSSTALWHSSQFSQTADEIYGFEQSPKVQSPILARRAAEYLVMPEKTLPTLPPAADFRSYSQRAGRRGLEDGLWESRHSEEVMTVKTVRVKEHGGEGDDIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFX4</accession>
    <name>A0A8H6FFX4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small COPII coat GTPase SAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Small COPII coat GTPase sar1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_008291</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPII-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155159.1">
        <property type="nucleotide sequence ID" value="XM_037299158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFX4"/>
    <dbReference type="GeneID" id="59336688"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00879">
        <property type="entry name" value="Sar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000161">
        <property type="entry name" value="Small COPII coat GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006687">
        <property type="entry name" value="Small_GTPase_SAR1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45684">
        <property type="entry name" value="RE74312P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10 22" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 22" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="13 22" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 20" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 19" id="KW-0547">Nucleotide-binding</keyword>
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        <ligand>
            <name>GTP</name>
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        <location>
            <position position="29"/>
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        <ligand>
            <name>Mg(2+)</name>
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            <position position="30"/>
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        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
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            <position position="32"/>
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        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
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            <position position="33"/>
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        <ligand>
            <name>GTP</name>
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            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
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        <location>
            <position position="34"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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        <location>
            <position position="35"/>
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            <name>GTP</name>
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        <ligand>
            <name>GTP</name>
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        <location>
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            <name>GTP</name>
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            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
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            <name>GTP</name>
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            <name>GTP</name>
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            <name>GTP</name>
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            <dbReference type="ARBA" id="ARBA00004299"/>
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            <dbReference type="ARBA" id="ARBA00004397"/>
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            <dbReference type="ARBA" id="ARBA00007507"/>
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            <dbReference type="ARBA" id="ARBA00019961"/>
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            <dbReference type="ARBA" id="ARBA00021124"/>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00023134"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00048548"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
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        <source>
            <dbReference type="RuleBase" id="RU003926"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6226850.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="189" mass="21592" checksum="B621AF4701AE0C05" modified="2022-01-19" version="1">MFIVNWFWDVLASLGLLNKHAKLLFLGLDNAGKTTLLHMLKNDRVAILQPTLHPTSEELAIGNCRFTTFDLGGHQQARRLWKDYFPEVSGIVFLVDAKDHERFAESKAELDALLAMEELSKVPFCILGNKIDHPDAISEEDLRHQLGMWQTTGKGKVPLEGVRPIEVFMCSVVMRQGYYEAFRWLSNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG91</accession>
    <name>A0A8H6FG91_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037299729.1"/>
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    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="303"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="653" mass="71860" checksum="C737BBA4C7DE6DCA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGQ7</accession>
    <name>A0A8H6FGQ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">60S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008666</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155532.1">
        <property type="nucleotide sequence ID" value="XM_037299531.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59337061"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_uL5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="6">
        <location>
            <begin position="17"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="7">
        <location>
            <begin position="74"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21332" checksum="391DB2FAA05C129E" modified="2022-01-19" version="1">MLICGHQAESQKDKSANPMRELRIEKLVLNISVGESGDRLTRAAKVLEQLSGQTPVYSKARYTVRTFGIRRNEKIAVHVTVRGPKAEEILERGLKVKEYELRKKNFSQTGNFGFGISEHIDLGIKYDPAIGIYGMDFYCCMTRPGERVAKRRRAQSKVGASHRINQQETVKWFKNRFEGIVRGDRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGZ1</accession>
    <name>A0A8H6FGZ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008908</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155772.1">
        <property type="nucleotide sequence ID" value="XM_037299771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGZ1"/>
    <dbReference type="GeneID" id="59337303"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002410">
        <property type="entry name" value="Peptidase_S33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF2">
        <property type="entry name" value="PROLYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00793">
        <property type="entry name" value="PROAMNOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="86"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="475" mass="53373" checksum="E8679A26AF62B2E8" modified="2022-01-19" version="1">MTRVEQTSLFAPGAGGVSLQANMSAAKLLGKRTHQMPGRLNVSELFFEVPRDYAKPQSGSLRLFARSVERFEKPVDASKKEVKQPPWFLYLQGGPGMSCKSPQSYAWTDYVLDKGYQILFLDQRGTGLSSTITARTLASEGGPAQQADLLKNFRADSIVKDCEAIRKTLTSDFPEEKKKWSIMGVSFGGFCAVNYLSRFPEGLQEVFIVGGLPPLVRDPYPVYNRLFRKVKQRNEIYYQKYPEDIHRVKDIVRHIQKEEDIKLPSGGTLSVLRLRQLGMHFGFHGSMDSVHDMITRFTSDLNQSGFFTRPTLAAFGALIPFDTAPLYALLHEPIYAQGTAPAWAADRCLAHHPDFARLPLDGPDPVLFTGEAVSRAMFADFDELRGLADVADRLARFPDWPPLYDGARLAANEVPVYAATFVEDMYVDFDFARETASRIGRCKTFVTNVLYHDALVNRTDELLKQLFALRDDTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI35</accession>
    <name>A0A8H6FI35_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007046</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156576.1">
        <property type="nucleotide sequence ID" value="XM_037297941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI35"/>
    <dbReference type="GeneID" id="59335446"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="8">
        <location>
            <begin position="94"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="365"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="398" mass="44087" checksum="789BB32F9391BBE9" modified="2022-01-19" version="1">MAPGIVDPPQTAPASDFAKASVGPKEAFIGGPQVFNKTAEEQGTEKQPPATHPKYLPVWDADTIYPPLEPFTHYEHGKDADPMSPEMFHEATSVTELTPSTGSEVRGIQLSALTKQGKDQLALFTAQRKVVVFRDQDLADLPIREALDFGSYFGRLHIHPTSGAPKGYPEVHLVHRGADDNTAMGLLKSRTNTVTWHSDVTYENQPPGTSFLYVLDTPTAGGDTLFSNQAEAYNRLSPEFQKRLHGLRALHSGHEQAQNSKSRGSIVRREPVTSIHPVIRTHPATGEKAIYVNPQFTRHIVGYKQEESDFLLKFLYDHIALGQDFQARVKWAPRTVVVWDNRVTAHSATLDWANGQRRHLARITPQAERPTETSFEAHPEKGSELTKNVVNGKNGVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI53</accession>
    <name>A0A8H6FI53_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156599.1">
        <property type="nucleotide sequence ID" value="XM_037297964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI53"/>
    <dbReference type="GeneID" id="59335469"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542">
        <property type="entry name" value="HELO DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542:SF3">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="148"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="599"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="953" mass="108887" checksum="0BAD851246CAE8B3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI82</accession>
    <name>A0A8H6FI82_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Ammonium-dependent carbamoyl phosphate synthetase</fullName>
            <ecNumber evidence="10">2.1.3.2</ecNumber>
            <ecNumber evidence="9">3.5.1.2</ecNumber>
            <ecNumber evidence="8">3.5.2.3</ecNumber>
            <ecNumber evidence="26">6.3.4.16</ecNumber>
            <ecNumber evidence="7">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Arginine-specific carbamoyl phosphate synthetase, ammonia chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Glutamine-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="36">Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="41" type="ORF">HO133_007110</name>
    </gene>
    <organism evidence="41 42">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="41 42" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="41">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="35">Multifunctional protein that encodes the first 2 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5) and aspartate transcarbamylase (ATCase; EC 2.1.3.2). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="25">Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="24">In the 2nd section; belongs to the CarB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21">In the 3rd section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="22">In the C-terminal section; belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23">In the N-terminal section; belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="41">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="10"/>
    <dbReference type="EC" id="3.5.1.2" evidence="9"/>
    <dbReference type="EC" id="3.5.2.3" evidence="8"/>
    <dbReference type="EC" id="6.3.4.16" evidence="26"/>
    <dbReference type="EC" id="6.3.5.5" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156638.1">
        <property type="nucleotide sequence ID" value="XM_037298003.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59335510"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01423">
        <property type="entry name" value="MGS_CPS_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000025">
        <property type="entry name" value="Bifunctional pyrimidine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000005">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000036">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000009">
        <property type="entry name" value="Carbamoyl-phosphate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF5">
        <property type="entry name" value="CAD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 37" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15 37" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="42" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ATP-grasp" evidence="39">
        <location>
            <begin position="610"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="39">
        <location>
            <begin position="1145"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="40">
        <location>
            <begin position="1402"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="38">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="38">
        <location>
            <begin position="1880"/>
            <end position="1973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="38">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="38">
        <location>
            <begin position="1907"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="38">
        <location>
            <begin position="1940"/>
            <end position="1965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00013008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00043979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00043998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00044031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00044249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00044318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00044334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00058513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00081752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="40">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="EMBL" id="KAF6228996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2294" mass="252226" checksum="409BCB90AAC5086F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIH7</accession>
    <name>A0A8H6FIH7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lon protease homolog 2, peroxisomal</fullName>
            <ecNumber evidence="8">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_007221</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9 12">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156749.1">
        <property type="nucleotide sequence ID" value="XM_037298114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIH7"/>
    <dbReference type="GeneID" id="59335621"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000002">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000091">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000039">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03121">
        <property type="entry name" value="lonp2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027501">
        <property type="entry name" value="Lonp2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001174">
        <property type="entry name" value="Lon_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="2 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="15">
        <location>
            <begin position="9"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="14">
        <location>
            <begin position="723"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="432"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Microbody targeting signal" evidence="8">
        <location>
            <begin position="924"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 10 12">
        <location>
            <position position="816"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 10 12">
        <location>
            <position position="859"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="480"/>
            <end position="487"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6229107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="926" mass="101560" checksum="82060BF3E46798F2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FII1</accession>
    <name>A0A8H6FII1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NECAP PHear domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156816.1">
        <property type="nucleotide sequence ID" value="XM_037298181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FII1"/>
    <dbReference type="GeneID" id="59335689"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13228">
        <property type="entry name" value="PHear_NECAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000465">
        <property type="entry name" value="Adaptin ear-binding coat-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012466">
        <property type="entry name" value="NECAP_PHear"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847">
        <property type="entry name" value="ATP-BINDING CASSETTE ABC TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847:SF9">
        <property type="entry name" value="NECAP-LIKE PROTEIN CG9132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07933">
        <property type="entry name" value="DUF1681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NECAP PHear" evidence="2">
        <location>
            <begin position="14"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="270" mass="28665" checksum="AE500C9F115751C0" modified="2022-01-19" version="1">MASSAPTPLPADAIQRVLFIASTVHIYNIPPLTSNKGYTASTWTADDNKRQIFTGRLRVIETAVPLPKDAGESLSTEILLEDPSTGELFAKAPYTDAGAVEAVLDSSRFFAIRVVGDGGMKATLGIGFEERGEAIDFGICLQESRKVLGLETPSEKKSAAKAKAEAEVKKDYGLKEGQTIVVDIGRKGRRKAGDDDAGAERSGGATTASFSTIPPPESLVNGDGGAMPMLAPPRSAQDVRAEKRRSRGFEPVEKTAAELGFDDGEFGEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIV2</accession>
    <name>A0A8H6FIV2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxyhypusine hydroxylase</fullName>
            <shortName evidence="9">DOHH</shortName>
            <ecNumber evidence="9">1.14.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LIA1</name>
        <name evidence="11" type="ORF">HO133_007443</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:14101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12592"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="ChEBI" id="CHEBI:91175"/>
            <dbReference type="EC" id="1.14.99.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the deoxyhypusine hydroxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.29" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIV2"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019135">
        <property type="term" value="F:deoxyhypusine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03101">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027517">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6229327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="356" mass="38723" checksum="E02720096340421A" modified="2022-01-19" version="1">MAPTAITPDFSKYPDLKIVPPSAPDQDPDSLPSEFEEPNPIAPLRAALVSEATPLAVRFRALFSLKHHASKHPPTLYTLPAIHAIAAALPSPSALLKHELAYCLGQTGNLAAVPFLRTVLEDRLEDAMCRHEAAEALGALGDWESLGLLRGLRDEAGEAEVVRETCEIAVGRIEWDHSAKRKSERLRTSDFTSIDPAPPLAQGTSQHTIEELQRQLLDTSFPLFGRYRAMFALRDLSSPPDLSTAVPAVKALASGFSDPSALFRHEIAFVFGQLSHPASVPSLVATLSNSKEESMVRHEAAEALGSLGDEEGVEDILRGFLNDPEQVVRESVVVALDMAEFEKNGEREYAVLPEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIX8</accession>
    <name>A0A8H6FIX8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alpha-1,2-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_007521</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157047.1">
        <property type="nucleotide sequence ID" value="XM_037298412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIX8"/>
    <dbReference type="GeneID" id="59335920"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6229405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="54555" checksum="6622A5B936055612" modified="2022-01-19" version="1">MISIRRSRTIVLPILLFSLIWLVFRGHLRWLHHPFTTAINGTSADEVPPSPFQSGLIEFWDTTSRALVEAKPDCSLPTDPIEAPLNEFASLKKGFDFRPDLLKISQQDVDKMRDAHARFVAQIPELAQKLPYATGTQGIVVAASGALLPVFLVSLKMLRRTGSTLPVEVFMESKNNYEKEICEIVLPPMNATCMIFSEILEAVPHRLKMSRYQLKALALGFSSFDDVLLLDADNLPVEQPENLLNSEPYLSKGFVSWPDYWANTASPKFYEISMQPVPPTSERAASEAGQLLLSKGKHASLLLLILYYNYYGPNHYYKLLSQSPAGERDRETDKETYLAAAMSLNASFHTVTTNIETLGYTQVHDNRFKGAGMVQHNPEDDYSKNILHNKEIKEVRPSFLHANTYRLDASEMHEIFNSEIRSQRMWGGTKGMIGKFGRDVEKQVWAETLYTGCHLEHAFSAWVNKTHVCKKIGRVYKFLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKA8</accession>
    <name>A0A8H6FKA8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U three protein 23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004397</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in rRNA-processing and ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UTP23/FCF1 family. UTP23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157315.1">
        <property type="nucleotide sequence ID" value="XM_037295314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKA8"/>
    <dbReference type="GeneID" id="59332805"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09865">
        <property type="entry name" value="PIN_ScUtp23p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000006">
        <property type="entry name" value="rRNA-processing protein UTP23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057776">
        <property type="entry name" value="UTP23_sensor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24779">
        <property type="entry name" value="UTP23_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP23 sensor motif region" evidence="9">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="187"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="311"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6230058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="320" mass="36260" checksum="2CB1F6CB509EAFD5" modified="2022-01-19" version="1">MRGKRSKQYRKLMAQFEMTFGFRGPYQVLIDAQIIQDTEKFKMDLVGGLERTLSAKVKPMITQCSMRHLYLLKHVPQPTKDALILTAQSMERRRCNHHVLDEPLSTLECYNSVIDPKNSHTNKNRYIVASQDDDVRRHCRGIKGVPLVFVKRSVMVMEPMAAGSVKAREGMEREKFRSGLKTRGIGLIGKRKRDEDDGDAVTGEGQEAKSMTESEERAGKKKKVKGPKGPNPLSVKRPKKATVSDHKEETRREGRSDEALSYPDAEERKSEIRVSAVGNLDGPFDETQDREAKRKRKRKHKSKQLEGLTAAINNGNEGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKE5</accession>
    <name>A0A8H6FKE5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157413.1">
        <property type="nucleotide sequence ID" value="XM_037295412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKE5"/>
    <dbReference type="GeneID" id="59332903"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="209" mass="22561" checksum="C508318E806C6CBE" modified="2022-01-19" version="1">MPVFEDSPSISKNLNNHLCLGLVTTQKPGSGHRISYIDSPEALGEARKEWMIDRSGPLSGYYLPQTNAYFQSESIVGSQELRELDITIQMALQADTKPSYETISPAAVVFTSTQGAGGSAGVKLESSDPTKLPKINPTLLSPPFDRGVALESVRETLEFLDKALLAKDQVHLATGPTGHNDGDISVPCLSFICIFSSHSGMRRQSGNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKU2</accession>
    <name>A0A8H6FKU2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004702</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157617.1">
        <property type="nucleotide sequence ID" value="XM_037295616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKU2"/>
    <dbReference type="GeneID" id="59333108"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34213">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2 (NTF2) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34213:SF2">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2 (NTF2) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="19713" checksum="29B33CE868D9E6BF" modified="2022-01-19" version="1">MSSGTSDLNIENTNIKTASGVSLDEHKKTLVGSVLDLFAGRPSLKKLQLWDDNGVFQDPITIAEGRKQYEAQWYGLQAAFSEIERLHHEVTSGGNPITMDLRTRYVVKGIGKEQTIDSKINIFHDKDTGKITKVEDKWDGKLPDSSLMNAFRKINAVTVPKVVGVPKSDEEDAKLGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL22</accession>
    <name>A0A8H6FL22_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004827</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037295739.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59333233"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056027">
        <property type="entry name" value="DUF7608"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF56">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF24581">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="750"/>
            <end position="884"/>
        </location>
    </feature>
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        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="93"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="181"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="425"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="666"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="996"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="448"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1029" mass="113993" checksum="DF2FBB326B49AA1C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FLK0</accession>
    <name>A0A8H6FLK0_9LECA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
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        <lineage>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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            <begin position="57"/>
            <end position="121"/>
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            <begin position="60"/>
            <end position="79"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="153" mass="16160" checksum="2D31D8F9AF13CDF2" modified="2022-01-19" version="1">MAYNSHISSPMPGFTAEQTTSLQAMIDASVKAAIEQAISQAMTAFETRQVVAQEQSIVEKTAEKPTEKTAEKSTEKTTENKSTGSSSPIQAGTLSTLPHQATTQNCGDSSARLNGAPPPTLASQLASQLASQLASLPGYIHYAEGFSEKIQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYQ8</accession>
    <name>A0A8H6KYQ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
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    </protein>
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    </gene>
    <organism evidence="5 6">
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        <lineage>
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            <taxon>Pezizomycotina</taxon>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229261.1"/>
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        <property type="term" value="C:CHRAC"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
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        <property type="entry name" value="Histone-fold"/>
    </dbReference>
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        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
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        <property type="entry name" value="CHROMATIN ACCESSIBILITY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="148"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="238"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
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            <begin position="245"/>
            <end position="258"/>
        </location>
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        <location>
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            <end position="324"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="330" mass="36624" checksum="0F67905178017F08" modified="2022-01-19" version="1">MANRILGFKSTRATSPQAVPPDSPLHARYLPRIFEEESLVPIRISRLSKKSYEVDTGTPPSKNFKAPSFETSQIFSHRWILLPPEELANAHTHPYSLDTQTPSSQTPYHPFQTLTLSDATYKHNRAQIATMPYNNTAIPPPEEITGVASLPLARVKKILHIDEEIIACSNNGAFAITIATEMFIRYLAEQALNVVKSEKKPRRNIQYKDMANAVARMDCLQFLEDVIPRTSTFREYKAKKASKATKGNQPLQNGQTTLDAGRSLPQRPIGTNEQNGAPVDDGKMSDGTISEADPEETRAPAPNTNGGLVFEHYEPNGSHRRDESGDVEMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYS6</accession>
    <name>A0A8H6KYS6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007449</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156975.1">
        <property type="nucleotide sequence ID" value="XM_037298340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYS6"/>
    <dbReference type="GeneID" id="59335848"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="141" mass="16019" checksum="9A47D0AF55D3A341" modified="2022-01-19" version="1">MTYRLSGNLDFPRPEDFLRSERLERLCQIKADLHHFETSEPQTLASIGEQAIEDTDSDLIEYYDEPFRTAVIAPKRPRADDEPWGLECAFSGFSIRAMATWGGGRPLMGFYAQLDFPPLISPKELIARRGNPLTSTRKAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZQ6</accession>
    <name>A0A8H6KZQ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Vps53 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004456</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VPS53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157374.1">
        <property type="nucleotide sequence ID" value="XM_037295373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZQ6"/>
    <dbReference type="GeneID" id="59332864"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.110">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 53, C-terminus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039766">
        <property type="entry name" value="Vps53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031745">
        <property type="entry name" value="Vps53_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038260">
        <property type="entry name" value="Vps53_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007234">
        <property type="entry name" value="Vps53_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16854">
        <property type="entry name" value="VPS53_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04100">
        <property type="entry name" value="Vps53_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034183631" description="Vps53 N-terminal domain-containing protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="domain" description="Vps53 N-terminal" evidence="9">
        <location>
            <begin position="227"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="Vps53 C-terminal" evidence="10">
        <location>
            <begin position="827"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1002"/>
            <end position="1044"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6230117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1076" mass="119979" checksum="0F6CB69A62B00D44" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5T3</accession>
    <name>A0A8H6C5T3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146904.1">
        <property type="nucleotide sequence ID" value="XM_037297703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5T3"/>
    <dbReference type="GeneID" id="59335207"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF8">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="1">
        <location>
            <begin position="70"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="309" mass="34587" checksum="31240B0A264C83B4" modified="2022-01-19" version="1">MATTAVTTRAYPKRKRAEISYHESSSDEVEVDDEYAASDEKLTARSRKKLKSSHTAVSTKTLPKRNIFPFTSLPAELKNQIYALSLIDPDGIFLISKTKQYRRTIQRSVPYTVNSSGYYRRSRAYRYQPQSLPLNTSPAATIPVLTPNVLLLNRAIYAEAQPILYAGNTFAVEDTTAMHAFLATIGPKNRATLTDLTIKGWGYTKAHKALNHPAFTLLAGAVNLTRLHIDCQIMWGGPKRIAKQLYRDGFHWLEAVGATKGRVDAAIELIEISEGILTTSYGYIRSQSDDVSAEEAMEEFRAALRRMLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6J2</accession>
    <name>A0A8H6C6J2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Killer toxin Kp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006581</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147189.1">
        <property type="nucleotide sequence ID" value="XM_037297479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6J2"/>
    <dbReference type="GeneID" id="59334982"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.10">
        <property type="entry name" value="Killer Toxin P4, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015131">
        <property type="entry name" value="Killer_tox_Kp4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011329">
        <property type="entry name" value="Killer_tox_Kp4/SMK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09044">
        <property type="entry name" value="Kp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55221">
        <property type="entry name" value="Yeast killer toxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034836681" description="Killer toxin Kp4 domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Killer toxin Kp4" evidence="3">
        <location>
            <begin position="218"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="366" mass="39525" checksum="9A48FCF17E4B758E" modified="2022-01-19" version="1">MSRSHQPRLSSAIVIALLCINSSIASPVANADRSHVSIIGASEPVHIILSEDLPPERSSLNGGSTMVNDGTTVTTSFKNRRNLAQRRDSLSIVSVSEPVHFIPIEDLPPERPFPNGGSTTVNDGTTVTTTFNDRRDMVRRRNSVSIASASEPVYVIPTEEIPPGHISNSGSSSTTNDGTTVTTTYTTPPNLLQTKSNVTGSVNLINVRNLTSPVSEIDMGLNCEGSVIMCIGSAQMGVMHTLRDYTYAIPTGYRYYAGQDIACMKHNVYPNPWITWGFYCIFMQGNIPAAGVDGALIQLKMQQMIEHHCLGCGSVPFSDDNDPRTLGILTVNYVRQSECEGLCYYRPPGIAANAAVKVPQGMTLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6V0</accession>
    <name>A0A8H6C6V0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147427.1">
        <property type="nucleotide sequence ID" value="XM_037297302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6V0"/>
    <dbReference type="GeneID" id="59334805"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="139" mass="15307" checksum="BF210470EEBCA12C" modified="2022-01-19" version="1">MTTTLYILYNADASVMGKLNYGYRKLTSPKDKPACSACDITHGGLHLEETAAWKEAKVTLVKEGHVEIRQMHRDELTSEIKSFVQEKKLSYPIILAGEGEGDLRVVMDRQELGECGGNAQQLAAKLREKGVVSSSHSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C768</accession>
    <name>A0A8H6C768_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147334.1">
        <property type="nucleotide sequence ID" value="XM_037297209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C768"/>
    <dbReference type="GeneID" id="59334712"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="472" mass="52586" checksum="A3E7D6D0B21CDA34" modified="2022-01-19" version="1">MNCPHPDSVIPPGTHCVNLEIRQCSDPKHFQLHYEPAANLRKIKQTPKGRGGLYGLGKHGEERYCVGCLEKMLEEHRRRLQRKEPQAVFLPGHPIFEIAYWIIGDQRVIPNRGGKFTLRSFKIYGIELWKARWLWNRCDANPDYVLTQTPGRTDMIQHLPGRYPRAIYLQPEDKEYRGLFDDNTVGEISSDEVADCSLTEVLGKLDACAVAHERLKAQLSAARKLVEKSAADARRAECHRKINERADRQRQWEEIEEASIIYKPRPRTVPIVRCSTPTPAPKSPRTAPVVGDDIIRKSSLVASDALLFSQNYEIGATLSAKSATAIPSTGQRHHDIQRIEMATDVPATNEHLARGIADGNRPLSIMTAWVTIHGTVRPPISKSARTSPKGNQKGDLVSDGVDDVVLEGRGKPGSKAIDPFEETDMGDSVDIRPREQSKGSGEYSEDDKKPFDTVTAGSTSLEKLLPATLENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7C0</accession>
    <name>A0A8H6C7C0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147713.1">
        <property type="nucleotide sequence ID" value="XM_037296532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7C0"/>
    <dbReference type="GeneID" id="59334029"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15488">
        <property type="entry name" value="Tm-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12030">
        <property type="entry name" value="Uncharacterised protein family UPF0261, NC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12020">
        <property type="entry name" value="Uncharacterised protein family UPF0261, NN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051353">
        <property type="entry name" value="Tobamovirus_resist_UPF0261"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008322">
        <property type="entry name" value="UPF0261"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056778">
        <property type="entry name" value="UPF0261_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044122">
        <property type="entry name" value="UPF0261_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002674">
        <property type="entry name" value="PRK02399.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31862">
        <property type="entry name" value="UPF0261 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G10120)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31862:SF1">
        <property type="entry name" value="UPF0261 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G10120)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06792">
        <property type="entry name" value="UPF0261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23189">
        <property type="entry name" value="UPF0261_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033271">
        <property type="entry name" value="UCP033271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0261" evidence="1">
        <location>
            <begin position="9"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="UPF0261" evidence="2">
        <location>
            <begin position="199"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="423" mass="45088" checksum="156976567F17D3F1" modified="2022-01-19" version="1">MAGTSSKPFVLLLGTCDTKLSELLYVRSHMLQHGVGVKLMDVGRNPVLHQDIDYTHAQVLSHLSVQRDTDSIERGQVIKTMTSAASSLVTELHSRKMIAAAICIGGSGGTSLGASVMRFALPIGFPKLIVSTVASGNVSNFVGETDITMMYSVVDVAGQNSILNPILENAAGMIAGAAKAFHDRSEKEKNDKVADEKRKKSVAITMFGVTTPAVEAARAQLESNGYEVFVFHATGVGGRAMEKLISQHRIDGVLDLTTSELADELVGGIMKGGPDRLTAAARAGIPQVVSLGGLDIVNFGPRASVPAQFKARLLYEHNPDTTLVRTSVDECRTLGKQIAEKLRTHVEKPDMARVFTPLGGISMLSTKGGPYYDKVADKALFDAVIQGLAGSGIAVVEDRRAFNDDGFSTDMAKVLTQFIEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7I3</accession>
    <name>A0A8H6C7I3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006491</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147514.1">
        <property type="nucleotide sequence ID" value="XM_037297389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7I3"/>
    <dbReference type="GeneID" id="59334892"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039751">
        <property type="entry name" value="HERPUD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943:SF27">
        <property type="entry name" value="HOMOCYSTEINE-INDUCED ENDOPLASMIC RETICULUM PROTEIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943">
        <property type="entry name" value="HOMOCYSTEINE-RESPONSIVE ENDOPLASMIC RETICULUM-RESIDENT UNIQUITIN-LIKE DOMAIN HERPUD PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="610"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="6"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="95"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="210"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="318"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="652"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="735"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="96"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="656"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="735"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="777"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="795" mass="84672" checksum="7E71F4B18E5816D5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7J2</accession>
    <name>A0A8H6C7J2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147848.1">
        <property type="nucleotide sequence ID" value="XM_037296667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7J2"/>
    <dbReference type="GeneID" id="59334165"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="538" mass="59288" checksum="F2E2B33D02B16A9D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7U0</accession>
    <name>A0A8H6C7U0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thiol methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005793</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147879.1">
        <property type="nucleotide sequence ID" value="XM_037296698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7U0"/>
    <dbReference type="GeneID" id="59334198"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008854">
        <property type="entry name" value="TPMT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32183">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32183:SF6">
        <property type="entry name" value="CYSTEINE SULFINATE DESULFINASE_CYSTEINE DESULFURASE AND RELATED ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05724">
        <property type="entry name" value="TPMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51585">
        <property type="entry name" value="SAM_MT_TPMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="30238" checksum="D84F306B9C7DCADC" modified="2022-01-19" version="1">MALQEDRARLMAHFAGDISKHQNKWSDLWDKGDFLPWDRGVPNPALVDTLTDQRDLLGTCFVEDGFGNKRRKRALVPGCGKGYDVLLLAGFGYDAFGLEVSETAVNRCYEEQKANGHKYPIRDESSGAGAVKFMHGDFFGTDWMTNIEGDGKVDLIYDYTFLSALPPSFRPDWALRMAQLLAFKGNLICIEFPSAKDPLIGGPPFALPPQVYTEHLAHPGKELPYDDKGHVKEGVSGPKSPVALTRVAHWRPMRTHDIGKGQDWVSIWRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8L0</accession>
    <name>A0A8H6C8L0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005957</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148041.1">
        <property type="nucleotide sequence ID" value="XM_037296860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8L0"/>
    <dbReference type="GeneID" id="59334362"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="144" mass="15953" checksum="47D41B481093B11C" modified="2022-01-19" version="1">MDAVDDYEARDKGWRMDLMARAWETQGKSTVLKAKKVEDYGKKTGQGEELGESCWHAGKIWSTSLGSRRDNAVKTSMRSGKTSDMVFLNELKPSDVFDCYHYTSAKSSLVEAYTDYGCLETQGKQWACISGLPAMGAMAIERGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C905</accession>
    <name>A0A8H6C905_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148587.1">
        <property type="nucleotide sequence ID" value="XM_037295889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C905"/>
    <dbReference type="GeneID" id="59333383"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025363">
        <property type="entry name" value="DUF4267"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14087">
        <property type="entry name" value="DUF4267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="142" mass="15250" checksum="5BBAE3BBEE47C589" modified="2022-01-19" version="1">MPNPWILRLAILFALYATFLSVYILVSPLPAIHLFGLEPYYSTPKSPSSLVPFITIFGGRNFALGLAMLALYWQGMYRAMGTIMICCTVSGAVDTVVMSLWGMAGKAMGHGVGCVLLGLTGWGLLESENAGGREKRSADAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C923</accession>
    <name>A0A8H6C923_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005469</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148361.1">
        <property type="nucleotide sequence ID" value="XM_037296379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C923"/>
    <dbReference type="GeneID" id="59333875"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034462362" evidence="2">
        <location>
            <begin position="26"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="152" mass="16380" checksum="065103283CEA460D" modified="2022-01-19" version="1">MRLINVCHVSALSAVLASLTVISSATPHTPLLTRKGDDVETKREQVSLSSDGTLGPLPLAKRVDINDLVSTELGQWTMHVIKYYALVPVESAAQALESFYSNIKRQASAMSEQSAAASLPARLNSKAGNLFTANSAFFQLKIWCGSSTQQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C956</accession>
    <name>A0A8H6C956_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148610.1">
        <property type="nucleotide sequence ID" value="XM_037295912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C956"/>
    <dbReference type="GeneID" id="59333406"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="222" mass="25239" checksum="B7F12BE0D0EEF8C2" modified="2022-01-19" version="1">MVYLPSSPNDRREDVGMAKAIFFKQTEYWIWCYRGKDKKPVPAASCWKGSGICRGRGSLRNIREIFSATGDAPVGWMRRGDLRMRGEATGALALSLSALATANMTIIAIVVDQQTRTKTLIARNDDQEKNFTHNELFTLQKKFLDNFIAPNNTIQAKSINSSLLADNVQGRVDITRTFDGRELNTEYLFYDPLPIADDVRSRVDITRTFDGVRWIQANPKWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9N7</accession>
    <name>A0A8H6C9N7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148869.1">
        <property type="nucleotide sequence ID" value="XM_037294822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9N7"/>
    <dbReference type="GeneID" id="59332311"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051531">
        <property type="entry name" value="N-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43792">
        <property type="entry name" value="GNAT FAMILY, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G00765)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43792:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="296" mass="32464" checksum="049D429190AD9FE7" modified="2022-01-19" version="1">MADTPSPNKSTRTPRLILREARKGDLAAIHKLYSNEERRSQPHDSVSRTATYLEAMVTSSTNGDLAFVLVLPPSATTITTSTTPAGTTIDEGKTIGHAGIWYADRNELVFMIDNAYWNRGYMTEVLAALVPIFWEKGLKKVYADANPDNGASLKVLRKCGFTQVGGNIVESLAGEYESLRMELANPRDGEEEGEFDFADEGAKWKSKNQALYRSSNPSTYHHTLHPYRPATHKRQAEPQSNKLIGKGKLSIAETHSAVPSEQDIGELLDEWGKQNAVRAAITGMGTVLGAWGVLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Y6</accession>
    <name>A0A8H6C9Y6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="4 10">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_003909</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="4 10"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148878.1">
        <property type="nucleotide sequence ID" value="XM_037294831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Y6"/>
    <dbReference type="GeneID" id="59332320"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000006">
        <property type="entry name" value="Mannan endo-1,6-alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF36">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016302">
        <property type="entry name" value="Man_a_manosd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034637035" description="Mannan endo-1,6-alpha-mannosidase" evidence="12">
        <location>
            <begin position="32"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR016302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6219443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="466" mass="50886" checksum="0412C616B6740857" modified="2022-01-19" version="1">MRLTPCSRKSCLSWLSTTALSLLAGSTLTTAITLDLQDPNSIKSAASTCAYNLLTFYTGNNTGDVPGNLPQPYYWWEAGAMFGILIDYYYYTNDSQYNDLVTQGMLWQVGPDNDYMTPNQTKTEGNDDQGFWGMAAMSAAEQKFPDPPSTKPQWLALAQAVFNDQASRWDNQYCDGGLRWQIFTFNTGYDYKNSISNGCFFNLGARLALYTGNDTYAQWAEKTFNWTQAVSLMDQNYHFYDGAHTTLNCSDINRVQWTYNAGVFLLGASVMYNYTNGSPLWQERVMGILNATSIFFATNPPDVMYEVACEPPNTCDTDNYSFKAYLARWLVASIKYAPFIQPTVMPWVQASATAAALQCSGGTNGQMCGMKWTQGSTWDGTQGVGQQMGALSIIQANLIDQVTVPVTNKTGGTSKGNAAAGGGGGISTSNPALSQNVITRGSKAGAGILTCLVLISVIGCSWWMIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAB0</accession>
    <name>A0A8H6CAB0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003975</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148941.1">
        <property type="nucleotide sequence ID" value="XM_037294894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAB0"/>
    <dbReference type="GeneID" id="59332384"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF158">
        <property type="entry name" value="MEMBRANE PROTEIN PTH11-LIKE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="50"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="371" mass="41162" checksum="B1BC9AB42E50A70E" modified="2022-01-19" version="1">MPDEMNYPDWPALAPPPGVLSNFNDPKTLSTSLLTDNAIFLSLMLIVVGIRLYSRAFLSHAVGWDDYMCLLAALCSAAHSFMTLWIIQYGFGRHLWDIRAITLLPSHLKQMGSMGIIGSASTLSIKISIFLFYLRLFRVNQRFRYWVHSGMLFCILYTATYWAFQTASIIECNTPQSLDIAICANSDITTVIVTVINVVADFYVLALPIGIVGRLNVKRTKKLGLIAVFLCGLIASLANVAKLIYATLHLHDQDSLWSAALTTEFAIVEMNLGIIAASMPALPQFFAKARIFHASTYSFLHRLLNRRDPSSLSWFGQSSKSLASGYPERQRGPAVRGESETEVQSLPDIRAYTRIDVSHDDIDLGGGLAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBB4</accession>
    <name>A0A8H6CBB4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149561.1">
        <property type="nucleotide sequence ID" value="XM_037294180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBB4"/>
    <dbReference type="GeneID" id="59331668"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="348"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="454"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="580"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="749" mass="83687" checksum="F1461E8769AFF5D7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBW8</accession>
    <name>A0A8H6CBW8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150160.1">
        <property type="nucleotide sequence ID" value="XM_037294082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBW8"/>
    <dbReference type="GeneID" id="59331570"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="175" mass="19429" checksum="542D3B64A3F76B06" modified="2022-01-19" version="1">MPGSCLAAASLTKALNLSFSSQDTKSLKDAKEDCRLGDNCTEKGARQSIKDDTREEEERLNPRLQKQRGVLQRTTDKTWGIGRSTSGLAKVDIIPRDVLTGQNTGGMEGAERTNEGESSSYGRQLTGLGKKKQEIFFWTDRGWGRLLRKPAMVSQTLLEGEALEKMKKEYAVEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCD9</accession>
    <name>A0A8H6CCD9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003107</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150109.1">
        <property type="nucleotide sequence ID" value="XM_037294031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCD9"/>
    <dbReference type="GeneID" id="59331519"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22453">
        <property type="entry name" value="KH-I_MUG60_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056553">
        <property type="entry name" value="KH_Mug60-KHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627:SF76">
        <property type="entry name" value="KH DOMAIN-CONTAINING PROTEIN YLL032C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627">
        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24563">
        <property type="entry name" value="KH_Mug60-KHD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="261"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="571"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="658"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="880"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="889"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="992" mass="110821" checksum="9319D662569FC967" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCV7</accession>
    <name>A0A8H6CCV7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyl hydrolase family 13 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150534.1">
        <property type="nucleotide sequence ID" value="XM_037293705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCV7"/>
    <dbReference type="GeneID" id="59331192"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033934">
        <property type="term" value="F:glucan 1,4-alpha-maltotriohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004574">
        <property type="term" value="F:oligo-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000025">
        <property type="term" value="P:maltose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11333">
        <property type="entry name" value="AmyAc_SI_OligoGlu_DGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000064">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF232">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 13 CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0462">Maltose metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="5">
        <location>
            <begin position="13"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="587" mass="67786" checksum="2494E4B950D479EA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCX2</accession>
    <name>A0A8H6CCX2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GCS light chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Gamma-ECS regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Gamma-glutamylcysteine synthetase regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Glutamate--cysteine ligase modifier subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002088</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a catalytic heavy chain and a regulatory light chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150668.1">
        <property type="nucleotide sequence ID" value="XM_037293015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCX2"/>
    <dbReference type="GeneID" id="59330502"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035226">
        <property type="term" value="F:glutamate-cysteine ligase catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000026">
        <property type="entry name" value="Gamma-cysteine synthetase regulatory subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032963">
        <property type="entry name" value="Gclm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13295">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13295:SF4">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="10">
        <location>
            <begin position="94"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="315" mass="34663" checksum="D06D03196324826C" modified="2022-01-19" version="1">MTKLILSTSNIMNGGPSIIRRPASEKSNVELINALRSNFLASMQAFAPATKGFSSSNGAERKTISQDVPYSEWTAREDGSLFVPSIDFAQSGLAEERSQYDITVKLFFLPHTSATERCRHSQEAIDLVLRELHMPSIDLLIVSFPGVSFDADDEDEEEPQWSQDGEGEDLSAMVETWQALENLHGKGTVKQIGLSEFGSERLEKFLPRTNVRPSVDQINVRDCCVVPKSLILYAKQEKIELLTHNDCTNILPSGTVRELLGNGDKGAGVLAGPEEGSKGLKGELEPQWVVKYTAVVRDRGVIENKGYFAMAEVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD03</accession>
    <name>A0A8H6CD03_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150637.1">
        <property type="nucleotide sequence ID" value="XM_037292984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD03"/>
    <dbReference type="GeneID" id="59330471"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="457"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="610" mass="65889" checksum="F646FBB4B93991F2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDF2</accession>
    <name>A0A8H6CDF2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002822</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150576.1">
        <property type="nucleotide sequence ID" value="XM_037293747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDF2"/>
    <dbReference type="GeneID" id="59331234"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47429">
        <property type="entry name" value="PROTEIN TWIN LOV 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47429:SF2">
        <property type="entry name" value="PROTEIN TWIN LOV 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0157">Chromophore</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="5">
        <location>
            <begin position="85"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="530" mass="59632" checksum="32470C04DE20D53F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDH5</accession>
    <name>A0A8H6CDH5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150798.1">
        <property type="nucleotide sequence ID" value="XM_037293145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDH5"/>
    <dbReference type="GeneID" id="59330632"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF283">
        <property type="entry name" value="RETINOL DEHYDROGENASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="21324" checksum="120354B0B0350C68" modified="2022-01-19" version="1">MARAIASKGPSLLVLIARSTSKAEAVSKQLGVDFLSVKTRVLSLDLSSQKSVRQAATELENITKAVDVLINNAGVMAVSERTLSKDGEDIQFATNYSPQSGARTVNITSAAHVLTHLRFSDHIFKGVPIPYEEQPNTAIASQMDMPELDPQGGYHPMIAYCHSNTVNMLFTTELAEMLKDKGIYPFTSAPGVVETEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDJ7</accession>
    <name>A0A8H6CDJ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Armadillo-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002343</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150922.1">
        <property type="nucleotide sequence ID" value="XM_037293269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDJ7"/>
    <dbReference type="GeneID" id="59330756"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039868">
        <property type="entry name" value="ARMD3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013636">
        <property type="entry name" value="ARMH3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13608">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13608:SF3">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08427">
        <property type="entry name" value="ARMH3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01158">
        <property type="entry name" value="DUF1741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Armadillo-like helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="643" mass="71750" checksum="BBAA20E29C9D3A69" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDT0</accession>
    <name>A0A8H6CDT0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150999.1">
        <property type="nucleotide sequence ID" value="XM_037293346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDT0"/>
    <dbReference type="GeneID" id="59330833"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="9"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="151" mass="16484" checksum="F4FA535B6456FD92" modified="2022-01-19" version="1">MENTYMDKIRGVEEIIGYQFSDPWTIWEALQAAGSPVQTIGNRRLVGGNRRLAVLGDRVLDLAIAEAWHEGMEARVRFDQIRQAVGGNLNLDRIGRVTGLVAFVNQNPSTLGVVSPVTMSATVEAVLGAVYLDSDMNTVKRVMNTLGLVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE12</accession>
    <name>A0A8H6CE12_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Prephenate dehydrogenase [NADP(+)]</fullName>
            <shortName evidence="9">PRDH</shortName>
            <ecNumber evidence="9">1.3.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001771</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>prephenate + NADP(+) = 3-(4-hydroxyphenyl)pyruvate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21640"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8 9">Amino-acid biosynthesis; L-tyrosine biosynthesis; (4-hydroxyphenyl)pyruvate from prephenate (NADP(+) route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the prephenate/arogenate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.13" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151238.1">
        <property type="nucleotide sequence ID" value="XM_037292699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE12"/>
    <dbReference type="GeneID" id="59330185"/>
    <dbReference type="UniPathway" id="UPA00122">
        <property type="reaction ID" value="UER00962"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3660.10:FF:000002">
        <property type="entry name" value="Prephenate dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3660.10:FF:000004">
        <property type="entry name" value="Prephenate dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000339">
        <property type="entry name" value="Prephenate dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3660.10">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046825">
        <property type="entry name" value="PDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050812">
        <property type="entry name" value="Preph/Arog_dehydrog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012385">
        <property type="entry name" value="Prephenate_DH_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20463">
        <property type="entry name" value="PDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036510">
        <property type="entry name" value="PDH_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0827">Tyrosine biosynthesis</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="423" mass="47512" checksum="F1C212D6A7D3E732" modified="2022-01-19" version="1">MESITVGIIGMGDMGKMYARRIGAAGWRVNACDKPEKYDSLNDEFADSSNVRIFHDGRLVSRSSDVILYSVEAEAIGRIVSQYGPSTKQGAIVGGQTSCKEIEIAAFEAYLPEDVQIISCHSLHGPKVDPKGQPLALIKHRASQQSLALVEKILSCLNSNVVYLTAQQHDQITADTQAVTHAAFLSMGTAWCSNNQFPWEISRYVGGIENIKCNIMLRIYSNKWHVYAGLAILNAEAKKQIRQYAESVTELFKLMLGGHREELEKRIKTAGAAVFGSGQKDLLLRDEVLDRFSLGKVPKERTPNNHLSLLAMVDCWWKLGIVPYDHMICSTPPFRLWLGVTEYLFRKPQLLEEVISTAIDDNTFRSDDLEFTFAARGWSDCVSFGDFASYQDRFERISKYFEPRFPEATRIGNEMTSVILEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEA8</accession>
    <name>A0A8H6CEA8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001983</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6222015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151450.1">
        <property type="nucleotide sequence ID" value="XM_037292911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEA8"/>
    <dbReference type="GeneID" id="59330397"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451">
        <property type="entry name" value="MARVEL DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451:SF1">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="6"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="170" mass="18060" checksum="33989069F212E702" modified="2022-01-19" version="1">MLDIILIALRAVQALFAIIVLGLTAHVASYGLTPSSDSFMVFASVWTLLILIYLALAPRFFSAVAHPMAMLALDALTMLFWFAGFIALAVYHHQAERVYLGDGLGDVYKGCTLAGGFCGEIEAAAIFGAFEWALFVATTALLALEFMRGRGGRSSKNAAGPSTFGGTTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEI8</accession>
    <name>A0A8H6CEI8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001888</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151355.1">
        <property type="nucleotide sequence ID" value="XM_037292816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEI8"/>
    <dbReference type="GeneID" id="59330302"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="268" mass="29411" checksum="6621B78D0FCEA0FD" modified="2022-01-19" version="1">MSPSTQNVTITVAQGTSNHGNPKLLCSLSKWTDIATFFLANSVAHAATVKSVPGEPALSSCLALVFVLLFPASDIRRGLSAIYQSAVLANTPLKTAATAKALCMVVRTIDWRPQTGDVVEVLDYMGPREVEKFIKHKSDFVFKTTKGKKFVDGDDWDWTRIDHVPAQILSRSEKLHFMLFKFYSYQKPRRKEKEQESSNVPALVARGVGVGPDSVTKGDFRKLSGLPRSTFGAKDRKVHGVWQLPEEYALCVVPSGAEGDEPPCGGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFU2</accession>
    <name>A0A8H6CFU2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151881.1">
        <property type="nucleotide sequence ID" value="XM_037292462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFU2"/>
    <dbReference type="GeneID" id="59329948"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="13657" checksum="5908ED7B8D62102A" modified="2022-01-19" version="1">MLAYSAYKGYKNHKDKKSSERLAAAAADESHGASPPPQQQQYLAPPQEQQQQQQHHHQQQHYAPPQQQQQQQQQYSAPIYAPRAPSPSEGKFSNAQPSLEARWQNLEARVSALEAREAARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG10</accession>
    <name>A0A8H6CG10_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sorting nexin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_000949</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for retention of late Golgi membrane proteins. Component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment. Binds phosphatidylinositol 3-phosphate (PtdIns(P3)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Prevacuolar compartment membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152244.1">
        <property type="nucleotide sequence ID" value="XM_037291882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG10"/>
    <dbReference type="GeneID" id="59329367"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07295">
        <property type="entry name" value="PX_Grd19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000030">
        <property type="entry name" value="Sorting nexin-3, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042138">
        <property type="entry name" value="PX_Grd19_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051074">
        <property type="entry name" value="Sorting_Nexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963:SF2">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="21"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6222898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="142" mass="16745" checksum="E49357C415AA01DF" modified="2022-01-19" version="1">MQAMPDARQQSFEEIYGPPENFLEIEVKNPQTHGTSRNMYTDYEIVCRTNIPAFKLKHSTVRRRYSDFEYFRDILERESARVTIPPLPGKVFTNRFSDDVIEHRREGLQRFLQIVVGHPLLQTGSKVLASFVQDPNWDRNSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG48</accession>
    <name>A0A8H6CG48_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152248.1">
        <property type="nucleotide sequence ID" value="XM_037291886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG48"/>
    <dbReference type="GeneID" id="59329371"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="268" mass="30235" checksum="FF331CC1553417C8" modified="2022-01-19" version="1">MNFSYTESEFPEHANNMHPFAQDTLRNEIWAYCLQFEDDVEIAVTAKRMMSCKGGLQNPGTEAPTALAPQLLRTNKQIRKEGCAIMCKRNNFSIVLNQASYLGYSSRIPLSISVAGGDLASIPSKWTSKIRHYVHHMRQLTLYVTLNPEHETFHTPSRMLLEDKKLEVAAIVGTALGELCEILSFSNNLKSLKVNFLALGTEQLTTGHEHKVLECLGQLKGLKHIEICGVPKDAKDYLENIMRLSKRSEKERKEFPSSRAGYSWGSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGC6</accession>
    <name>A0A8H6CGC6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Outer spore wall protein RRT8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001074</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152368.1">
        <property type="nucleotide sequence ID" value="XM_037292006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGC6"/>
    <dbReference type="GeneID" id="59329492"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059112">
        <property type="entry name" value="CysZ/EI24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF2">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07264">
        <property type="entry name" value="EI24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="30547" checksum="2E47EF42BD51E834" modified="2022-01-19" version="1">MADKVRDVAVGEADRIKTLTVQAARSAAYLYPVKGIYYFLSHRSLWKPLTTKLAPTMTLGLGVTTFMFLFTYVPQVAVMAFTSGPLAAVSAALLVLSESSTLFTILSKTFLIEDALTDTFDGVLVSKNTTNLVSEGRQIKGGSDIMGKLGKLTKKPFQKFTPTAIIRYFMYLPLNFIPVVGTVLFVVLQGRRAGPAAHSRYFQLKQWNSTQKQKHIEEYKAAYTSFGVAAVLLELVPIASILFAFTNTVGAALWAADIEKGNVTTEGTAPGLNEQARKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGG2</accession>
    <name>A0A8H6CGG2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152256.1">
        <property type="nucleotide sequence ID" value="XM_037291894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGG2"/>
    <dbReference type="GeneID" id="59329379"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="142" mass="15903" checksum="1C5988BB48C4D090" modified="2022-01-19" version="1">MDSAYSEAGHGPGPRTRQRRKLDSVLPSLGSGSKKRKLIVPKKDQDSINMLAGYVSELKDMLHGLGIEDNNDVFVTADEATVTNADIYTVASPNGKWLPDSILRFVTQHFHSQFDPKGDYISLICPAECQWISFVEFFVRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGL5</accession>
    <name>A0A8H6CGL5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152157.1">
        <property type="nucleotide sequence ID" value="XM_037291795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGL5"/>
    <dbReference type="GeneID" id="59329279"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="147" mass="16616" checksum="27D22653D1AB8B62" modified="2022-01-19" version="1">MAFQGREGTLLSFRPLDNNLCNLPSFRKQPGSVPHWTLEVEGMDPQRGSSFIRTIRNQPSVSSILSHYHSFPPFIPVSSVRISHHKLYHHHQRHSPLLERCTTTNRSSDTQRLSELRALFSTLTTATNSLASSVHSLSKKSFPGVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHJ5</accession>
    <name>A0A8H6CHJ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">25S rRNA adenine-N(1) methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000233</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of an adenine present in helix 65 in 25S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BMT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152608.1">
        <property type="nucleotide sequence ID" value="XM_037291173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHJ5"/>
    <dbReference type="GeneID" id="59328652"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016433">
        <property type="term" value="F:rRNA (adenine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03044">
        <property type="entry name" value="BMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021867">
        <property type="entry name" value="Bmt2/SAMTOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008:SF1">
        <property type="entry name" value="25S RRNA (ADENINE(2142)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SENSOR UPSTREAM OF MTORC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11968">
        <property type="entry name" value="Bmt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="289" mass="32238" checksum="A54C8028A6C3FFF0" modified="2022-01-19" version="1">MAAKKQRRPQLLSSTRPRNIKPTLSLSSRTTRSLIRSHHTLRKQVSAALAKGDTSEAQAIQAKIDASGGLPKYQEASKQGQSAQRGGDTSKILTEWLTMGEGEDKISVESGKRLRMLEVGALRPDNACSRSGLFDMERIDLHSQHPDIKQQDFIERPTCAAGDLDQEGFDIVSLSLVVNFVGDPVERGEMLRRVASFLRPYSGRKVGLFPGLFLVLPAPCVANSRYLDEERLNAIMESLGYKRAKRKLSAKLVYYLWKYEAGKAEGSKVFKKEEIRSGKSRNNFAIVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI05</accession>
    <name>A0A8H6CI05_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cytosolic iron-sulfur protein assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CIA1</name>
        <name evidence="6" type="ORF">HO133_010524</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat CIA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI05"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03037">
        <property type="entry name" value="ciao1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028608">
        <property type="entry name" value="CIAO1/Cia1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="60"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="192"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="464"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="393"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="481" mass="52973" checksum="8F4B3157F151DBF9" modified="2022-01-19" version="1">MPSASSLPQISRLATLEPSSPSRAWLTAPHPTLPLLATCSSDKTVRIYSLTSFTLLSTITGGHKRSIRTCAWKPNLKGESVLATGSFDASVGIWRRYDNDMNPQPKDVLSGLDFDARDKAIASGQMGDEHAEEDEEDDDEWRFAIVLDGHESEVKSVAWSSGGNLLATCSRDKSVWIWEEVGDDDYETIAVMQEHEGDVKCVAWHPEEELLASASYDDDVRLWREDVDDWGCCGVLRGHGSTVWCVGWEGVVPRVFGDVEDGQKDTKERWVKRREASGPRLMSCSDDLSIRVWRRRPKEKVQQSRLSIIRSGTSEEDWVEEGQLPKAHSRPIYAIAWSKSGRVVSTGGDGAVVIYEERWKKAGGNAESKVTEAHEGGMGGAEVIGESKLDGAEPMEEVTNDTGSTSEADGQQGKQAEMEETPDATEWVVIAQIEGAHGVFEVNHVAWAPRRDKNGGSNVEQEELIVTTGDDGTVKVWTLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI52</accession>
    <name>A0A8H6CI52_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152994.1">
        <property type="nucleotide sequence ID" value="XM_037301360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI52"/>
    <dbReference type="GeneID" id="59338898"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02231">
        <property type="entry name" value="cupin_BLL6423-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047142">
        <property type="entry name" value="OryJ/VirC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36156:SF2">
        <property type="entry name" value="CUPIN TYPE-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36156">
        <property type="entry name" value="SLR2101 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="2">
        <location>
            <begin position="95"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="201" mass="21820" checksum="B41A00D12247FDCE" modified="2022-01-19" version="1">MSDPRPRNDTQLSQGLPGLTHHITGHDASGLAIVESDRPATWTPRINDTMAFDVVYTTSTFPPNMNGNADLTAHDKLIQSDELGLVNPNGTVARMVDFGPGTEALMHRTQSLDYGIVIEGEVEMILDDGVKRIMRRGDVAVQRGTNHGWRNVSSTEWARMFFVLQDAQKIKIGDKELGADFSNAGSDTEGLAGGQDVGSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI93</accession>
    <name>A0A8H6CI93_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153054.1">
        <property type="nucleotide sequence ID" value="XM_037301420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI93"/>
    <dbReference type="GeneID" id="59338958"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025040">
        <property type="entry name" value="DUF3984"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13136">
        <property type="entry name" value="DUF3984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="310" mass="33504" checksum="2B6F86801A94845A" modified="2022-01-19" version="1">MDRPNRSHPTLGPLTTLPTSPHASPSYLYPSASIPTTPSLLSRPSSQTRLRPTRSTPTAVPAPARKKTRTVRNPDDTPDDWVTRTASALTLTRLEEKGQAWLAARSSSTSLTGGEVGDDEYDAHSPEWSPSRVQSARGSNAKLRGLSRAQSRVQSRRGSVGGGNGVRGEEEEEEEEEEEEVGPDFVNAEEGEEEGEEVDEGEMKRVVMGRVGGWVDWAVGWMEFRGEGEEEGDADEAAGAAGEEDDGGEGIVRGELDPVELQRRLRRKKRKDDEAETGGEGTNIMGSAPEQAGVWSDAKWLLGVATKVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI98</accession>
    <name>A0A8H6CI98_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_010674</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153160.1">
        <property type="nucleotide sequence ID" value="XM_037301526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI98"/>
    <dbReference type="GeneID" id="59339064"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000008">
        <property type="entry name" value="Translation initiation factor 2B, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051855">
        <property type="entry name" value="eIF2B_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="107"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6224100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="487" mass="52559" checksum="AC99D6BCD1ADFE74" modified="2022-01-19" version="1">MPSSAGVLTPALTTFSRSVRNRGTPIESSIENLISLLKRRQIRDSKPCAMATAYLLRRVVETFKIADVSGLIERVQQVGERLLAAQPRELAIGNIVRRVLGVIREEAEEDRDGETSGYSDADTDSRPHSAGENGSRLPEAPGAVSRALLSSPLRRGASDQPSLEPHEDGAFQRLPVLTAHASYASTNTGPVVTSMFSLLSHPRSNVALPTATPGSQSAESHPPLSNTALANLTAAKDLRAEIVEGIQEILEELNQADDQIAGYCLEHIHSNEVILTHTSSITVQKFLLKAAAKRKFTVIHAETFPNHHESTHATVIGKAKGESDDELRPESFQKSLTAAGITVILIPDSAVFALMSRVNKVILDTHIVLANGGLVAAAGAKVVAKAASMHRTPVIVLSGVYKLSPVYPFDIDSLMENGDPAGVIGYGDGDMVNKVEFENPLFDHIPPELVDLYITNLGGHAPTYLYRIIADHYRAEDTELDKLNAYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CID3</accession>
    <name>A0A8H6CID3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCZ1/INTU/HSP4 first Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010687</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCZ1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153173.1">
        <property type="nucleotide sequence ID" value="XM_037301539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CID3"/>
    <dbReference type="GeneID" id="59339077"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013176">
        <property type="entry name" value="Ccz1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056:SF0">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="3">
        <location>
            <begin position="14"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="368"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="398"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="798" mass="87909" checksum="D8034AF155992977" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIQ7</accession>
    <name>A0A8H6CIQ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AHC1-like C2H2 zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010684</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6224110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <source>
            <dbReference type="Pfam" id="PF25909"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
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            <begin position="193"/>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAF6224384.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
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    <sequence length="352" mass="41053" checksum="956C41272C3E9377" modified="2022-01-19" version="1">MPLHLLGKKSWNVYNADNIAKVKRDEAAAAAREEGEEQRMQEVDAERRIQILRGLPFEAPRAPIEEERNERSRENRPDRERKRKRIAGEDDTDRDIRFAQENNALVPAKAEMQMKTKKSSDAPITDVKGHINLFPVDGTRHNAPKNAEVEAEKAKKQKEFEDQYTMRFSNAAGFKQAIGQKPWYHKINAVEDERGEVEAPSKDVWGNEDPRRKEREKIRTVADDPLSAIQKGVAGVREVERERKKWKEEKGREIRELEEAERRRERRRRRRREADDVEGFRLDDGAKPDEPTRRHHGSKDDSSPHSHRRRSRSPDRSGHRSHHHRHRPVIVDNRTGWEVGPGGRYSSQFAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ95</accession>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">WasteWater1</strain>
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    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
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    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224231.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
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            <end position="80"/>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1011" mass="111203" checksum="A2FD4CA69E8AB7F4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJK9</accession>
    <name>A0A8H6CJK9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153439.1">
        <property type="nucleotide sequence ID" value="XM_037301805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJK9"/>
    <dbReference type="GeneID" id="59339346"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034302476" evidence="1">
        <location>
            <begin position="28"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="264" mass="28521" checksum="83E0A68EC0258C81" modified="2022-01-19" version="1">MPPFFKLFHQLPMLLAILNLRFLLIHATPTSHASQLSSLSNDLSPRTQPLYSAALTSHPKRAQVTKGTFYRGLWSLALTTATFLAQGPSSSVAPASDVEFFAQQILDKVATRIATGATEKTAFGWGLEGISIAFASWIDPVTNVAPGVPVRYTFRGNSSPFMLCYESSGMSQEFSAPKTCLVTQNSTRKSNADSEIIKWEVVQEFVTRFVLWRAQRGTVLAFSGNIWGPGGQCIKIVLEVAGTTGFAEVASNILDSASEIHGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKA0</accession>
    <name>A0A8H6CKA0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153875.1">
        <property type="nucleotide sequence ID" value="XM_037301058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKA0"/>
    <dbReference type="GeneID" id="59338595"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01228">
        <property type="entry name" value="EGGSHELL"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="427" mass="41627" checksum="A05E63079E9A8FD6" modified="2022-01-19" version="1">MSGYGDDQQSGGGYGGGDSGMSGGQGGQSGGDSGSGGYGGGQSGGDSGSGGYGGGQSGGMGGDSGSGGGYGGGQSGSMGGDSGSGGGYGGGQSGGMGGDSGSGGGYGGGQSGGMGGDSGSGGGYGGGQSGGDSGSGGYGGDSGSGNQSSGGSGNQSGGGSGGGLKGKLMGMAENKFGGGGGGGGSSNNGNQGGSNWRDFYDCPLHLTTTTSNPVCMDPNQCTSGALLDRASADVAQLLRRFENIIAFESVRVALLQQRVGRSRIYGRLTCGGVCRTASKIAMPLPWGPIRWRSRRRHWYDIYNLPIAPNGFKNDGAYMIVARFYGSRAPFSVRVGSVLTVFCPQIRAAEDILSLTRILKEAWLFGKLQTVGSSEAETRAEAAAVKVAAGLSRLRGGNGAEATGSNQEPTGSNGDGRGVGKEGEERTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKB2</accession>
    <name>A0A8H6CKB2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cell division control protein 42 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010099</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153772.1">
        <property type="nucleotide sequence ID" value="XM_037300955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKB2"/>
    <dbReference type="GeneID" id="59338491"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01874">
        <property type="entry name" value="Cdc42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000236">
        <property type="entry name" value="Cell division control protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037874">
        <property type="entry name" value="Cdc42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="22288" checksum="6DB53A27936ECEDB" modified="2022-01-19" version="1">MVVATIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGDEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVTSPASFENVREKWFPEVHHHCPGVPCLIVGTQTDLRDDPQVREKLAKQRMQPVRKEDGERMAKDLGAIKYVECSALTQYKLKDVFDEAIVAALEPPPTKRGGKDGKKHRGCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKF7</accession>
    <name>A0A8H6CKF7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010375</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154045.1">
        <property type="nucleotide sequence ID" value="XM_037301228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKF7"/>
    <dbReference type="GeneID" id="59338766"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="6"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="343" mass="37176" checksum="3DE233CF9A89DF83" modified="2022-01-19" version="1">MPTQHGSLPSRQASQTTGRKSRALHTTRSARYPFVLGGDLAGEIVEVGKGVTRFRIGDRVLGHALGMDGERNRSSEGAFQQYTVIRDNMAASIPESLSYENACVLPLTLSTAATGLFQKDCLALKYPSVPPKPNGEALLVWARSSSVGSNAIQLATAAGYEVFTTASPKNFAYVERLGASQVFDYRSPTIIRDIILALKDKTLAGVLAIGNSGAESCIAVLEKCKGRKFVAMASFPWPETLPEGSGQTLAIISLMFSVVRWQILMWLKSKIKGIPTKFFIGETLQNNEVSHVVYENFLPQALTEGTYVTAPEPFVVGHGLEYIQEAFDVQKKGVSAKKVVVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKX4</accession>
    <name>A0A8H6CKX4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010281</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153953.1">
        <property type="nucleotide sequence ID" value="XM_037301136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKX4"/>
    <dbReference type="GeneID" id="59338673"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF15">
        <property type="entry name" value="SPORULATION PROTEIN RMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="506"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="4">
        <location>
            <begin position="307"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="207"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="532" mass="58995" checksum="2D4155655C6BB60E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL81</accession>
    <name>A0A8H6CL81_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154242.1">
        <property type="nucleotide sequence ID" value="XM_037300394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL81"/>
    <dbReference type="GeneID" id="59337928"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034917426" evidence="1">
        <location>
            <begin position="17"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="232" mass="24389" checksum="2D204B5DB175877F" modified="2022-01-19" version="1">MMRLFLLSSLFSTALSIVLPAERDTVAFEVAGRDLVERAGSHSTSVTMDPTRLSYGNLKPADVMQHLSGPCNDQLTGCDYENTGSLNIPSEMYVDATTIPIAGTIKLNPKGDFTLGNGSQFVDCIVKSVPYGAQCKTQSWNQPTLNPKLPDPPNSVGTASFCTMSNFVECSRTDTASGDSAGYLQVGVSFTADAGSSFDFCSILSLFSGLTGVLGPFFGTAAWACDTGSGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLD7</accession>
    <name>A0A8H6CLD7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009522</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154231.1">
        <property type="nucleotide sequence ID" value="XM_037300383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLD7"/>
    <dbReference type="GeneID" id="59337917"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF4">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="417" mass="47645" checksum="8E6EF27FEB12A0E5" modified="2022-01-19" version="1">MDPEASSMENCNIQTAPFLKRLPIEIRTEVYRNLLSTQYTKHLSVSRQLPIHAKLWPGHAYHLEPAILQTSRQVHNEASTVLYHENLFVRVRSTKKNLGRLMNLKGIHVFLKGRKAETFRHCAMSVDFDRLNTALRIKSQEYEPCYVIASTDLQLLCQSLLIQHTVIGSIFKHYEFRVTIHRMFDDMLSKTPARTSSQRRLLEPFTVLHGVSHFAITGPANTEYCASIIEQVSRMAPAVEECFSSAIELRDKGHEYVLQNDLEGAVKVYELAFSQLITTCLRCRLAGTEWLALHPELPGAIVDTYLTLVVRLAFTSYRLDAFEDVHLWACDATRFDPNGRAMRRENSYAKLVYLKATASARLGRHPQAVEELCEGLKLVTKEAYKDKQLLAMRREMRYQIKGLGGIRLLKAMGIGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNQ3</accession>
    <name>A0A8H6CNQ3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155213.1">
        <property type="nucleotide sequence ID" value="XM_037299212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNQ3"/>
    <dbReference type="GeneID" id="59336742"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="401" mass="43887" checksum="60EFDF816F822034" modified="2022-01-19" version="1">MDDNTRSPLTKSPTINIPPEPSTVNYEAQDKKDDSEYQTYIERKPQVQVNTIVAAFIGAHDGPTTLLIVYYAGHGKPGSYYGSLVLHGLFEYLAATKEMATTQVPGEDSFTSALIYALEALVEEKGMSTTVELLRKIKSHAPNFPSDQIPVLSDRRDDAQAGRIMLHPLHRNQEDGSRTAISSEETSNLDALKRHTVTLHSDFAKKPPSTDIEILGRHLNQIFERSTLGVNRVRWGGMKRSAAARAVGSFQAGLQRSRRASMKQWPIFATSRDSPQALELAATGSVGVNPPVVSAISFPNSSSSNEESDDGHILVRRVRNKKQKSDTDGEEASRTTHRLSVGSHCFTVGSPAETLVKLSAPSSVDKIGLAPGCLCRRQSTQAVLGFHSSDTRCKRRKDTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPG2</accession>
    <name>A0A8H6CPG2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008645</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155511.1">
        <property type="nucleotide sequence ID" value="XM_037299510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPG2"/>
    <dbReference type="GeneID" id="59337040"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF134">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="64"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="176"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6227203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="246" mass="27603" checksum="BEBDB1AE78E5C4E6" modified="2022-01-19" version="1">MGETQGKMYPLATVLTLLAAVAVLLRLHAREIKNAVLAWDDYLILFALIYLMDVRKNKLLTKAARIFRGALFLALVWTMITIIVTWTVGFFFATMLHYYPISKNRTGFGGTADTYIDENMMYIGQAFSDAITDLMILVMPIPWEVNAYTLKDHCGKRYEAHVFLKIAVGITSSKKPLRDAGNLEDRDNSSSAVSSEGSIYAGEDHNGGQYLGRDQVYVLKKTLEKVVDVGTKRNAVEQRYHASGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPU9</accession>
    <name>A0A8H6CPU9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Presequence protease, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Pitrilysin metalloproteinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_008567</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Degrades mitochondrial transit peptides after their cleavage in the intermembrane space or in the matrix, and presequence peptides; clearance of these peptides is required to keep the presequence processing machinery running. Preferentially cleaves the N-terminal side of paired basic amino acid residues. Also degrades other unstructured peptides. May function as an ATP-dependent peptidase as opposed to a metalloendopeptidase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer and homodimer; homodimerization is induced by binding of the substrate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M16 family. PreP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155434.1">
        <property type="nucleotide sequence ID" value="XM_037299433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPU9"/>
    <dbReference type="GeneID" id="59336963"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000013">
        <property type="entry name" value="Mitochondrial presequence protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000009">
        <property type="entry name" value="Presequence protease, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000011">
        <property type="entry name" value="Presequence protease, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013578">
        <property type="entry name" value="Peptidase_M16C_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055130">
        <property type="entry name" value="PreP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016">
        <property type="entry name" value="PRESEQUENCE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016:SF13">
        <property type="entry name" value="PRESEQUENCE PROTEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08367">
        <property type="entry name" value="M16C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22516">
        <property type="entry name" value="PreP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01264">
        <property type="entry name" value="M16C_associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16C associated" evidence="17">
        <location>
            <begin position="462"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="984"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6227126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1004" mass="111683" checksum="49596E04ADBCEA75" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPV9</accession>
    <name>A0A8H6CPV9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155782.1">
        <property type="nucleotide sequence ID" value="XM_037299781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPV9"/>
    <dbReference type="GeneID" id="59337313"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034319845" evidence="1">
        <location>
            <begin position="29"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="213" mass="23729" checksum="95FCFEC6A7E3FB66" modified="2022-01-19" version="1">MELLRISCPASQLALLFSLIHLSALCTAITLPHDVHTVAFTKLVRHEEHPIQPSTQPFSTQPIAKALSTTNTTSPTPPLYIFHLLDLRSIVASPETEHKVFRAMERTCEVFAYCLLDLDLLDQQDLVLRLGDFTLDFHDMAEHLSILAVKAVVMKLMQMIIKGLFGFVQGEMVDAHAGVRMIFAFGVLGVDYENRALRKRRAGEKGMTPSSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQB3</accession>
    <name>A0A8H6CQB3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008825</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155689.1">
        <property type="nucleotide sequence ID" value="XM_037299688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQB3"/>
    <dbReference type="GeneID" id="59337220"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09864">
        <property type="entry name" value="PIN_Fcf1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000019">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037503">
        <property type="entry name" value="Fcf1_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="66"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="194" mass="22539" checksum="E77699679DD95B10" modified="2022-01-19" version="1">MGVTKKTRKFVQVKRIIGQRDARLKKNQDKAVIQSKRKSKDEIVREIPQVSSSLFFQYNTALVPPYSILVDTNFLSLTVQHKLEILPTMMDCLYAKCIPIITGCVMAELEKLGQKYRIALRIARDERWERLKCDHKGIYADDCLVERVQKHKIYIVATNDRDLKRRIRKIPGVPIMSVARGKYVIERLPDAPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR57</accession>
    <name>A0A8H6CR57_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155898.1">
        <property type="nucleotide sequence ID" value="XM_037298562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR57"/>
    <dbReference type="GeneID" id="59336089"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111:SF2">
        <property type="entry name" value="SPLICING FACTOR YJU2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="346" mass="39028" checksum="56F22DB8F3049B3C" modified="2022-01-19" version="1">MQGFNMGRYVPPEHEGTTSANKLAGKHALGSRARKANQGILTVRFEMPFPIWCTTCPKPTIIGQGVRFNAEKKKVGNYHTTPIFSFRMKHVACGGWIEIRTDPMNTAYVVTEGAKKRDTGEDKFQEGEIKITTEEERARLEGDAFARLEGKVEDTQRTVSDKSRIEELYNAKGRDWDDPGQANRRLRKVFRVGRKEREKNGKATESLKDRMSLGMDLLEETEDDRKRAGFVEFGEAGGEGAIAKAKSRPLFADIRTLDKPLAKKIKTTKTVLEAEKRRDILRKELADNTRVSLDPFLSMDKPNVQALGGVPLIQRKRKVEDRGTFSKSNDPVDVTRPPALVEYDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRM9</accession>
    <name>A0A8H6CRM9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156261.1">
        <property type="nucleotide sequence ID" value="XM_037298925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRM9"/>
    <dbReference type="GeneID" id="59336454"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022127">
        <property type="entry name" value="STIMATE/YPL162C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735:SF1">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12400">
        <property type="entry name" value="STIMATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="42652" checksum="2283C0C8188EE1DB" modified="2022-01-19" version="1">MSPDDVVLPTPAAHSLPAEMVEMVFSPTLSYIAASTFRHSSSLSTITPTSPMNLTSSGESRPGAENGECALLGPFALFIQGALGVLALLVLVFKRWRERPQRPIKIWAFDASKQVVGSALLHIANLLMSMLSSGQLTIKAGDYQANPCSFYLLNLAIDTTVGIPILVVLLRLLTHGFSLTPLGNPPESIQSGNYGRPPKTQWWLKQSFIYFLGLLGMKACVFFIFQICPWIIRVGDWALRWTEGNEMVQVFFVMLFFPVVMNALQYYIIDSFIKDQKPSDHDPIPHEDGDEDSIEEHGRTRRRRSHGEDYDGARSIVAGNETTKDGAEITVAEGKDKPKLKMEPRKLDEYDPAVDGEGSGSGGDLENPPDPSKEGKAKMQQGHAKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRU0</accession>
    <name>A0A8H6CRU0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156326.1">
        <property type="nucleotide sequence ID" value="XM_037298990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRU0"/>
    <dbReference type="GeneID" id="59336519"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="49403" checksum="08402DEFA6730780" modified="2022-01-19" version="1">MQTQQNRSKQPTTSFHPSNPTMDRNPSSRYGNPLTFNVAVMFLFYILSVSLCLSSFSTCICVIFNPVGTFRTITNYLFYALGLSILLTLLFVALTGVCFDNQALPPSRSIQETMGETLVPEEAPSSQDVSDTAPIQEAHHEVSAPQQTVDATPIQEAHREGSAPQGVLETTLTQKAYREVSVQPTPRLSDCAALHAHQAECERLRRAFRSPFIEAQATAIHRLTDADTCVANPAARNDGAPPFTSAASTSRQTALEDDHEKGQIAIAKTVDGVPRFCEEILASPEEMSDAGEQEENALEAKAANRTETAGKDKVAGDVITARESKKIEKWISQTNLANIRTVKACCGTLKGVDANDGDIDEQTPEIMVWRCKDGGALSETRSLSQDSNFQKALRGYMGKKLDHGGRRSRSGNSTPSLGLSEESEFQGWVREMFTLEKVGKRKAGEKRRARSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6R4</accession>
    <name>A0A8H6F6R4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SDA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_006714</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for 60S pre-ribosomal subunits export to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the SDA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147047.1">
        <property type="nucleotide sequence ID" value="XM_037297611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6R4"/>
    <dbReference type="GeneID" id="59335115"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027312">
        <property type="entry name" value="Sda1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048292">
        <property type="entry name" value="SDA1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007949">
        <property type="entry name" value="SDA1_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012977">
        <property type="entry name" value="SDA1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730">
        <property type="entry name" value="HSDA/SDA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730:SF0">
        <property type="entry name" value="PROTEIN SDA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21638">
        <property type="entry name" value="SDA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05285">
        <property type="entry name" value="SDA1_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08158">
        <property type="entry name" value="SDA1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SDA1 N-terminal" evidence="9">
        <location>
            <begin position="69"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 middle" evidence="8">
        <location>
            <begin position="547"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 C-terminal" evidence="10">
        <location>
            <begin position="702"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="504"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="675"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="504"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="564"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="675"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="696"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="746"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6217612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="755" mass="84470" checksum="4314E81F6BE0DE8E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6W0</accession>
    <name>A0A8H6F6W0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006527</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylthioribose kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147135.1">
        <property type="nucleotide sequence ID" value="XM_037297425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6W0"/>
    <dbReference type="GeneID" id="59334928"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34273">
        <property type="entry name" value="METHYLTHIORIBOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34273:SF2">
        <property type="entry name" value="METHYLTHIORIBOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6217700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="330" mass="36786" checksum="EA293E6FA81E204F" modified="2022-01-19" version="1">MTEITNTEQLKAYLGKLGNAKIDLTSLQLLAGGTANFVWRLLERSGKRIIIKHAEPYLKTAPGLSFSVDRMDFEHRAMTSVPPLLSPSSVVAVPEVYQYDSLSHVLMIEDGGARTLKEAYADPTLDTPTFGKALGRWLAGLHQRTKHTAIGDNQAAKSMYRFAYTHLAVALQKYDQDPSLAEQIDAQYGSLLQTDDECVCHGDFWPGNVLINDQGRLSIVDWEMVRRGCGATDVGQFAAEAHLLDRFRGGKGLRTTFLEGYRWTGELDNRFLKRVAIHMGVHLAFWPTRVSWGTETETKEIVKVGCELMKRALGQDESWLAGSLLNELQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7E1</accession>
    <name>A0A8H6F7E1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Conidiation-specific protein 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000029">
        <property type="protein sequence ID" value="KAF6217414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146849.1">
        <property type="nucleotide sequence ID" value="XM_037297826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7E1"/>
    <dbReference type="GeneID" id="59335330"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034431549" description="Conidiation-specific protein 13" evidence="1">
        <location>
            <begin position="19"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="30662" checksum="6851059F43C6B7FA" modified="2022-01-19" version="1">MRATFIGLAGSLVPLALTQRLDKPPLETGLDYLQKGLLEYLGPVKSSYKKWTPGWIPSDCKTMTVNANLSVAHVETFSVQYEDVGTSVNVAFSEISNQCPNDPWLLCRHIDSPDPLENMIDLFGRVPVRARQWVRHIVNLPDPTNFTSAYNWAGNIAMFESINRDLTLFIHETGHSLDLLGAYPDNPLSTSRHWHAQYAKDSHVPDPYSQSNIYEDLAQNTVVAAYDLNVPGGIGVVEPKWRDVLHQFDTIEVEQRKAGGFAGERGSVHEAFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7H7</accession>
    <name>A0A8H6F7H7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial phosphate carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_006680</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147013.1">
        <property type="nucleotide sequence ID" value="XM_037297577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7H7"/>
    <dbReference type="GeneID" id="59335081"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000024">
        <property type="entry name" value="MIR1p Mitochondrial phosphate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF12">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="29"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="127"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="225"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6217578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="33889" checksum="ECC71DEC7B55BD02" modified="2022-01-19" version="1">MAASTGSTKLDGLVQKASANTPEKLSGLALYSRFALAGAICCSVTHGGLTPVDVVKTRIQLDPVTYNKGMIGGFRQVIKNEGYGALATGFGPTAAGYFLQGAFKFGGYELFKQQCITYFGYETASNNRTAVYLASSALAEFFADIALCPLEATRIRLVSQPTFASGLLSGFGKIYKQEGVGAFYSGFGPILFKQVPYTMAKFVVYEKVAEAVYQRVDKSKTSDGMQTVINLGSGLMAGFAAALVSQPADTMLSKINKTKGLPGEGTTSRLIKIAKELGLRGSYTGIGARLFMVGTLTAGQFAIYGDIKKFLGATGGVEIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7P7</accession>
    <name>A0A8H6F7P7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006615</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147223.1">
        <property type="nucleotide sequence ID" value="XM_037297513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7P7"/>
    <dbReference type="GeneID" id="59335016"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF50">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="100"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="157"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6217788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="733" mass="82659" checksum="47E5657782E4294F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7Q0</accession>
    <name>A0A8H6F7Q0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA recombination and repair protein Rad51-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147782.1">
        <property type="nucleotide sequence ID" value="XM_037296601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7Q0"/>
    <dbReference type="GeneID" id="59334099"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19490">
        <property type="entry name" value="XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030547">
        <property type="entry name" value="XRCC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="42443" checksum="29B478860DD44745" modified="2022-01-19" version="1">MSVEGLGRDLLGEVKVECLDELLRALRELDASSGPSLGFPPLDRLLDVFQNPPPTRQQQHYQTQWSVQQEGPAPPPKEKPLPVIEIAAAAACSGKTQLLYQIILLSLLPVEHKDEPINGRGNAVVLLDLSGRFSVLRLHHLMHNHISSICSRSSLTLPEQEISSLISDSFTHLHIFHPQSSSSLLSTLAALPSFLLSSPSTHFSANRPLGLLAINDLSAFLWQDRLDADEGTGLPASNHAENANNSLFLERYHNLISSLRHVQHLFSCPIVATNWGLAPVTSVAGHAALRPHLPSLWNNFCTTKLVVVKDRVSKFGPDLSAEEAKREGAQRWEAVEKSGFSGWINWWGNEGWREEVREGIRELKGGGGFSFRVTEDGIMVHDEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7Z5</accession>
    <name>A0A8H6F7Z5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147781.1">
        <property type="nucleotide sequence ID" value="XM_037296600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7Z5"/>
    <dbReference type="GeneID" id="59334098"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF5">
        <property type="entry name" value="BTB_POZ DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="23276" checksum="5362CDD98D7033E1" modified="2022-01-19" version="1">MSSHIFPFVFPAAIQKAYEAGLYTDLKIICGNSEFMVHKIIVCSQSKVFHATCSNGFMESSTNTIDLTADGISLVSRMIHCLYHGTYADFDAIEDAQDWKSGYQLHAGMYALGDKYDLSVLKDTALVNFKLATKAKPKDRLGLIESIPIVYSSTPDSDRNLRDAALVKFKTYPSHFLHNDVKASFQKVLLEVPDFSWDLHQYWMSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F803</accession>
    <name>A0A8H6F803_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147553.1">
        <property type="nucleotide sequence ID" value="XM_037296979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F803"/>
    <dbReference type="GeneID" id="59334481"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034007808" evidence="1">
        <location>
            <begin position="24"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="373" mass="40612" checksum="B8B6D1D0121F47E8" modified="2022-01-19" version="1">MYSTYSHILLPILLSLTSRIVLAQEIAKPNYDQGWCYYCSDDDAPPLCNAQCTVAINSLCGQDLTQSQVVTEQNCTLQYKPPVNPDLNRNGAIPPSPTAYQCNITFNNILASCGRDAGSPNSATVNESYCTTSGGGGTYGWNDDGSVMTDGLGRYIVSTTGTDQCGQAEASWQQATSVILWNDSWIGPNDQVVLDTNPPPLTGTAAALATTLPTPNPECDTEVCDIYDNPYYATSPVGPWPEGGKNSLRHRIIYEGWSEAPNSQRLPNSIEDRCGVAGGNYAAYMNGTQNVADFDLPADLCWCVPDAIFDASVGIQLPDDTFCPAGTTLQPGQEVNRIELRRLMERGLKIRDPTRVVERDLTKEVKHLNGRHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F815</accession>
    <name>A0A8H6F815_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_006043</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148127.1">
        <property type="nucleotide sequence ID" value="XM_037296946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F815"/>
    <dbReference type="GeneID" id="59334448"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033597">
        <property type="term" value="C:mitotic checkpoint complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.900.10:FF:000002">
        <property type="entry name" value="Mitotic spindle assembly checkpoint protein MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF11">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="8">
        <location>
            <begin position="16"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="228" mass="25682" checksum="526A7B671E6CD14A" modified="2022-01-19" version="1">MSAEKDKSKVHKLSLKGSSKLIAEFFEYSINTILFQRGVYPAEDFTAVKKYGLNMLVTSDDQVKAYIKKIMSQLNKWMTGGKISKLVVVITSKETGEHVERWQFDVQIFNRPSKKAASKDTSKPPDKENAAPIDTPEPPPPEKTEKEIQLEIQSIFRQITASVTFLPQLDAGNCTFNVLVYADADSEVPMEWGDSDAKEIENGEKVQLRSFSTSNHRVDTLVSYRLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F886</accession>
    <name>A0A8H6F886_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147808.1">
        <property type="nucleotide sequence ID" value="XM_037296627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F886"/>
    <dbReference type="GeneID" id="59334125"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="232" mass="26286" checksum="5AC614209334457F" modified="2022-01-19" version="1">MSQIFTTLPLEVRNEIYSHVFHYDSITPKSNNTGKLLHLNTRPELDDTFLLYKTSDPQKFLALLCVNHQISDEAAAFFYGKTNFHGGWHEVAAFIKGIGAHRRDMIRSVEISHPAWLAFAIDKDENFELLSGLPNLRRVRIAASVRGFTHLQRALIRGGILELAGKFDIGVYNTCNKSNLSDATPPVPQFYTDRYVWSCARGTTQWTGGERIRTIIAQSQTKRQCWVLTGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8T1</accession>
    <name>A0A8H6F8T1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aromatic amino acid aminotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005502</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148393.1">
        <property type="nucleotide sequence ID" value="XM_037296411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8T1"/>
    <dbReference type="GeneID" id="59333908"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF1">
        <property type="entry name" value="AROMATIC AMINO ACID AMINOTRANSFERASE, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="66710" checksum="678808D6B5F1BC71" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8V7</accession>
    <name>A0A8H6F8V7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148920.1">
        <property type="nucleotide sequence ID" value="XM_037294873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8V7"/>
    <dbReference type="GeneID" id="59332362"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="162" mass="17772" checksum="975AF833B15F822D" modified="2022-01-19" version="1">MDQLPIQPATQPAIHSVTQSDILQEEEQYSIERIAETVSSSSDTTGLLSTISHQSTQSATQSATQPDTQQEKKSLQQANAENTTENTAENPAEKSSGSKAPIVKNIAQLSTIPRQIATQQAENTTTIWPIIPTALIWYITAMSTQEEDLLRRSQPSHYNHGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9H4</accession>
    <name>A0A8H6F9H4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Late embryogenesis abundant protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149098.1">
        <property type="nucleotide sequence ID" value="XM_037295051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9H4"/>
    <dbReference type="GeneID" id="59332541"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372">
        <property type="entry name" value="DAUER UP-REGULATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372:SF11">
        <property type="entry name" value="RE19971P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="23201" checksum="B838103A9DE4D652" modified="2022-01-19" version="1">MVKVLENRAVDLDIVPGSKDGGELKDQADAITGQATQNLPPEARQRVEGLTQGATGVGADALQTVGGGVKGIVDTAGNTVGVLGGGLSDTVSGVAGGLGKVASGAGQTAAAPFTSAETKEGGEGAADSAKGAADSAIDTAGEAGGKASETADEAGGKVSETAGEAGGKASETAGEAGGKVSETAGEAGGKASETAGEAGGKVSETAGEAGGKASETAGEAGGKASEAAGSAKDTVGDAAGEGQKKLGLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9I3</accession>
    <name>A0A8H6F9I3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149039.1">
        <property type="nucleotide sequence ID" value="XM_037294992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9I3"/>
    <dbReference type="GeneID" id="59332482"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="248" mass="27021" checksum="094C4B66BBDD1420" modified="2022-01-19" version="1">MPVLRSGSTVSFVNRRNARRHGFRVPVKPNPQQTRPKANSTGAQSRNRSTPVDLAALTRNIRYRQRARANGLATGDSADTLREKRRWVQTEFDASRINGKATPELHDLYNKRNIGKPQHQRKMAQSLSDAAKEAKRRLEEVFGQRSGLRAVAGAVGHSLGDDSGDAAIAQPSDGEAVHTTKRPAAHLTADNDAAARQLVLAKGYELADYSGGNQGIGAEADNTEEGEEEEGDEIEIKMEEDADEGLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9T5</accession>
    <name>A0A8H6F9T5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149701.1">
        <property type="nucleotide sequence ID" value="XM_037294320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9T5"/>
    <dbReference type="GeneID" id="59331809"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="188" mass="20783" checksum="35DD4A3AE6B2B8DD" modified="2022-01-19" version="1">MATFPHPAIPLRQESYTSAAEPSMGTEPRYFGRVKAVNTTPPSFTIIPEDRTSRPDYDLAFGLSNIQLVEFLNLFASGEMRVSYVLRPGPTGALLVQDVQSAETVIVKEEGSPSWSADGPGEGKVERGKDVTSQVGFLASQHDRITLEEEARRNAKGEFRNRMGGLPSHFRAMKWDEWNAEGGRARRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAM1</accession>
    <name>A0A8H6FAM1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002245</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150825.1">
        <property type="nucleotide sequence ID" value="XM_037293172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAM1"/>
    <dbReference type="GeneID" id="59330659"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1170:FF:000001">
        <property type="entry name" value="Endo-1,3-beta-glucanase Engl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000006">
        <property type="entry name" value="Endo-1,3-beta-glucanase Engl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="9">
        <location>
            <begin position="11"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="10">
        <location>
            <begin position="337"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="698" mass="76919" checksum="79628E2466D2B027" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBF5</accession>
    <name>A0A8H6FBF5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001686</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151153.1">
        <property type="nucleotide sequence ID" value="XM_037292614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBF5"/>
    <dbReference type="GeneID" id="59330100"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000051">
        <property type="entry name" value="Protein tyrosine phosphatase (Pyp1), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF561">
        <property type="entry name" value="PROTEIN TYROSINE PHOSPHATASE 36E, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="306"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="559"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="763"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="201"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="673"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="682"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="695"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="905" mass="99802" checksum="A65E94F12D1094B0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBK9</accession>
    <name>A0A8H6FBK9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II transcription factor B subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001821</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TFB2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151288.1">
        <property type="nucleotide sequence ID" value="XM_037292749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBK9"/>
    <dbReference type="GeneID" id="59330235"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2610:FF:000001">
        <property type="entry name" value="General transcription factor IIH subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040662">
        <property type="entry name" value="Tfb2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004598">
        <property type="entry name" value="TFIIH_p52/Tfb2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00625">
        <property type="entry name" value="tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152">
        <property type="entry name" value="TFIIH, POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03849">
        <property type="entry name" value="Tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18307">
        <property type="entry name" value="Tfb2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor Tfb2 C-terminal" evidence="10">
        <location>
            <begin position="390"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="462" mass="51367" checksum="4CF8FF112130AEA6" modified="2022-01-19" version="1">MSASSLRSFEYLEGLPGTTFNRLYKQPSTALAIFRRMLPHLAKSFVMALLYISHPMPVTDLEAWVRPDGRRERDNAVNLLERLHILAAATNPGKPRAYALTNPFAASLRLALTGGGNHHSFGVPYSTSEEDTMSITDLDEFARRQWEGVLGYMVGSTGMNIASEGVTLSQGVKILLASGRLVEARGKTVDITKDGFAFILQEVNAQVWTMLILYLQNAESLNMDEVDVLAFLFMLGSLELGLPYSKANLTQTQANMLEDLGDFGIVYQASPDSSYFYPTRLATTLTSDAGGLRSSSFASPADNNRGFIVIETNYRVYAYTSSNLQIAILQLFARLITRYPNMVAGKVTRESIRRAVSMGITSDQIISYLTTHAHPQMAKNQPVLPPTVVDQIRLWQLEGDRMKATVGFLFKDFNSQAEFQAPCRYAEEIGVLVWKHEGQRKFFVTRHEQVAAYLRNKSGKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBR5</accession>
    <name>A0A8H6FBR5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151468.1">
        <property type="nucleotide sequence ID" value="XM_037292049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBR5"/>
    <dbReference type="GeneID" id="59329535"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="183" mass="19632" checksum="7A597EDE46B78FC1" modified="2022-01-19" version="1">MTFVIQAAAVSKAIPAPAASTLSTQDGVNAVYAGLPDFLAAVKNQTRSGITNTKKYWDRGSWGYGDSKYSWTKTMNRNSYVMLAVDKLGNAALSHADLVYDVQLPLQMETVHSMIMQIKAGNAGISIPSKLIFVSHSSDSIVNADPAQTHPDHVDAAVLTSSLSGEANNKGDIPSCLYGKKWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCF5</accession>
    <name>A0A8H6FCF5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152529.1">
        <property type="nucleotide sequence ID" value="XM_037291094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCF5"/>
    <dbReference type="GeneID" id="59328573"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="136" mass="14935" checksum="2B3C500BD4B72F41" modified="2022-01-19" version="1">MGKLDSQRQGGSKDIASPAIHGLELILAAVYQGGFRRLVIDCDDDEVCGPEERLCGLEDQVGQGLEIAFGVEEDFREGEDKGRDAEEDVYDIDAIGKETALAERRCLNLFQDIAKMGESTESEGFDEQNGTWIGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCM2</accession>
    <name>A0A8H6FCM2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acyltransferase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152309.1">
        <property type="nucleotide sequence ID" value="XM_037291947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCM2"/>
    <dbReference type="GeneID" id="59329432"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050879">
        <property type="entry name" value="Acyltransferase_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23028">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23028:SF128">
        <property type="entry name" value="ACYLTRANSFERASE 3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Acyltransferase 3" evidence="2">
        <location>
            <begin position="20"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="460" mass="51441" checksum="BCB4F163DAD4A82F" modified="2022-01-19" version="1">MKRLLPWEAVPASTGQRNVLWAEGLRGIASLLVVTSHLSRSFAYTLITPAQDSTHTGSFIHLPLLRLPAQGAPWVSLFFVLTGYVNAMKPIKQARNGNGSQALSGLAFSAFRRTGRLVLPATIATIISWVICQLGLFEIGRTCEAVWIRDTSPKPGPGVTGSIRLLLANLFTTWTTGFNVYDKNQWTFPFLLKGSMLIFITLLATVRIHSNYRILVFLGLYAFNWAGGDPMLGMNIYFGALLAELSNRLPPPSMTPQRNPFIRAMTGVALLLGLHVSGYPEVSPEWTSWSQNLAQIGQFIFPGKDYWRYWTSIGAQLITLAVVISPRMQDMLSHPAFVWLGSVSLPLYLIHGPLVRSLLAWMLFGWQKPVSYYSQDVNGNLTDPFQRRSFPDGWVFCIVLPVFFLILLWASHLWTTWVEPWCASMTNRIEVMCGTETGEKGDLRRTNGCHGDKGERVTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD45</accession>
    <name>A0A8H6FD45_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010514</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153000.1">
        <property type="nucleotide sequence ID" value="XM_037301366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD45"/>
    <dbReference type="GeneID" id="59338904"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051615">
        <property type="entry name" value="Transcr_Regulatory_Elem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313:SF81">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="35"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6223940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="727" mass="81076" checksum="F78403443409F9D7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD81</accession>
    <name>A0A8H6FD81_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010772</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex. Involved in pre-tRNA splicing, probably by affecting the interaction of pre-tRNA with splicing endonuclease.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037301623.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59339162"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CRM1p Major karyopherin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="26"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1044"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1044"/>
            <end position="1056"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1073" mass="123070" checksum="98B61E1C1B002AC6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDC8</accession>
    <name>A0A8H6FDC8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sfi1 spindle body domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010924</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153407.1">
        <property type="nucleotide sequence ID" value="XM_037301773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDC8"/>
    <dbReference type="GeneID" id="59339314"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013665">
        <property type="entry name" value="Sfi1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08457">
        <property type="entry name" value="Sfi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sfi1 spindle body" evidence="2">
        <location>
            <begin position="367"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="936"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="957"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1030"/>
            <end position="1049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1067" mass="124883" checksum="766A04B7755AEBC2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDL0</accession>
    <name>A0A8H6FDL0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_011027</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153510.1">
        <property type="nucleotide sequence ID" value="XM_037301876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDL0"/>
    <dbReference type="GeneID" id="59339417"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF25">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01148">
        <property type="entry name" value="Xi_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="168"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="118"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="136"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="338" mass="39244" checksum="DBA60299B309BC58" modified="2022-01-19" version="1">MPEKASIHKHADADGQFRRKPSQFRDSISSSPDSEFPPEKDRYALYINQGCPWAHRANLVRSLKGLESIIQLIVCDFELTKEGWLFTGRDGSDEKDPLYGFTRMQDLYKKADPEYEGRFTVPALWDKKAETIVNNESSEIIRMFYSAFDELLPEHLREDGKPGGGFYPEKLQKEIDAMNDWVYNTVNNGVYKTGFASTQDAYEANLYPLFESLDRLEKHLGEPGHHPYLFGEYITEADIRLYTTMIRFDVAYYNIFMCNLKMIRHDYPRLSRWLRNLYWDGGKTLNGHAFKDTVNFWTYKYGYLRSKGRAEPDAKLVVPKGPVPDIQPLDDDKVTNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDS9</accession>
    <name>A0A8H6FDS9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HAUS augmin-like complex subunit 6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153664.1">
        <property type="nucleotide sequence ID" value="XM_037300847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDS9"/>
    <dbReference type="GeneID" id="59338383"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028163">
        <property type="entry name" value="HAUS_6_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14661">
        <property type="entry name" value="HAUS6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAUS augmin-like complex subunit 6 N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="699"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="779" mass="86670" checksum="C2B6E51D22AC9962" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE26</accession>
    <name>A0A8H6FE26_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="3 9">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_010262</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic component of the histone acetylase B (HAT-B) complex. Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. Involved in DNA double-strand break repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the HAT-B complex composed of at least HAT1 and HAT2. The HAT-B complex binds to histone H4 tail.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 9"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153934.1">
        <property type="nucleotide sequence ID" value="XM_037301117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE26"/>
    <dbReference type="GeneID" id="59338654"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013523">
        <property type="entry name" value="Hist_AcTrfase_HAT1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21184">
        <property type="entry name" value="HAT1_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038084">
        <property type="entry name" value="HAT-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="14">
        <location>
            <begin position="7"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="11">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="11">
        <location>
            <begin position="211"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="448"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="251"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with histone H4 N-terminus" evidence="12">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6225067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="490" mass="56058" checksum="BAB5B8A55480777C" modified="2022-01-19" version="1">MADQEEWSSNANEALEISFVQAAPGSLKTLSSFHPQFTYPIFGDEERIFGYQSLKLNLRFAAHDLRPNLEVLYDKKFKAVGDTKATDIEETLKEWVPEYTFEKLGAFTSHIQNDTSASSFKPPGELLETYQSKGRNFEIWSGELTDPTVQRLVERIQIMISFFIEGGTPLLLDDQDWTLARWRVYFVYEKLSNLPSPNTSPYSIVGYSTSYRFVTYVPTASSKSSTQDFTLPPPTPISPASLPSRARISQFLILPSHHSHGHGTHLYNAMVKTFRASHTCTEITVEDPNEAFDDLRDYCDYKHLLDNGTLARIFLRTDIDPKLSARKIGVRVPTSKLLDMPLLETLRKKNKIAPRQFARVVELYLLTKIAPHNRRAGTARLTQRARATDPNDRAFYYWRLLVKQRVYKKNKDVLIQLDRGERVDKVEQTVGEVAGDYERLLRGMEKWQGAEASGSLDKRDRAKRKVIDEDDEDEDEEMAGDAKRARSEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE52</accession>
    <name>A0A8H6FE52_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_011100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153583.1">
        <property type="nucleotide sequence ID" value="XM_037301949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE52"/>
    <dbReference type="GeneID" id="59339490"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034757883" evidence="2">
        <location>
            <begin position="19"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="183" mass="19863" checksum="2DF38E2362522C16" modified="2022-01-19" version="1">MLLRLATVVLCLSAISSGLPAAIRLREADAKAGSELGALMMKPQSLDYVKPREADAKAEPKPETIQMMSESLDYVKPREAAAEADLEPGSIQMMSESLDYVKPREAAAEADLEPGSIQMMSESLDYVKPREANAEAEAEPEHMSAMSQSLDYVKPREADAEAEAEPEHMSAMSQSLDFIKVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFD6</accession>
    <name>A0A8H6FFD6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154654.1">
        <property type="nucleotide sequence ID" value="XM_037299828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFD6"/>
    <dbReference type="GeneID" id="59337361"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="240" mass="26045" checksum="301F21A1E60AFB76" modified="2022-01-19" version="1">MARYYSLTLAFNTLHSLKIVLNNGSNTSVDSGLALAVLVTFAHLAFVQHLHGPYNTHYQRNLQRTLLPPSFTAEQTASLQAMITAAIDDAISQAMPAFETRLTAFETRLTAIEAKFRPAEYQVTAPQHRPWQQSIVEKTAEKTAEKSTEKTTENKSTGSSSPIQAGTLSIIPHQATMHYCGEFTIRLGNAPLLASLLASLLASFLASFLASLLASQLASQLASPLGYIRYAKDMEATGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFG2</accession>
    <name>A0A8H6FFG2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154729.1">
        <property type="nucleotide sequence ID" value="XM_037299903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFG2"/>
    <dbReference type="GeneID" id="59337437"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="503"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="524" mass="58502" checksum="EF81D6B6EB0C5A0D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGK7</accession>
    <name>A0A8H6FGK7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome component ECM29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008805</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155669.1">
        <property type="nucleotide sequence ID" value="XM_037299668.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337200"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024372">
        <property type="entry name" value="Ecm29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055443">
        <property type="entry name" value="HEAT_ECM29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF19">
        <property type="entry name" value="PROTEASOME ADAPTER AND SCAFFOLD PROTEIN ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23731">
        <property type="entry name" value="ARM_ECM29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13001">
        <property type="entry name" value="ECM29_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24492">
        <property type="entry name" value="HEAT_ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome component Ecm29 N-terminal" evidence="5">
        <location>
            <begin position="15"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome adapter and scaffold protein ECM29 HEAT-repeat" evidence="6">
        <location>
            <begin position="1326"/>
            <end position="1487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1867" mass="205657" checksum="49C53749B4813A64" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH84</accession>
    <name>A0A8H6FH84_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156421.1">
        <property type="nucleotide sequence ID" value="XM_037299085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH84"/>
    <dbReference type="GeneID" id="59336614"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14291" checksum="F5414E0758925AD5" modified="2022-01-19" version="1">MVANIIDLIRSMPEEEQVLMFVQFDNLMHNIVSAVNAEGIPNYGFTASSGRAVRNMVDDFQNRGPTKKILVLNWANSLAAGVTTVHIYDFLALQVTDVDLYQAQDRKTFVRQSVNGDSQWEMVDPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI12</accession>
    <name>A0A8H6FI12_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">serine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_006984</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156515.1">
        <property type="nucleotide sequence ID" value="XM_037297880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI12"/>
    <dbReference type="GeneID" id="59335384"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000026">
        <property type="entry name" value="Seryl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="142"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="56"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="272"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="288"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="359"/>
            <end position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="10">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6228873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="462" mass="52637" checksum="BCA8AA3225CE8A52" modified="2022-01-19" version="1">MLDITDFIAERGGDPERIRESQRRRGEPVEIVDEIIQMFEDHRKTNYGVTKIGSKVNDTQKEIGKKKKAKEDAEDLLKQKEALQKEKAAQEASAAEKLQALHLKAKTVGNYVHESVPISNDEANNKVEKEWAPEGFQKQKKDCLSHHEVLYRLDGYDPERGVKIVGHRGYCLTGYGLFLNLALVNYGLEYLFSKGFTPNQPPFFMNREAMAKTAQLSQFDEELYKVTDDGTEKTDKYLIATSEQPLSALHESEWLTEKDLPIKYAGYSTCFRKEAGSHGRDAWGLFRVHQFEKIEQFIFCKPENSWEHLEEMLANSEGFYQSLGLSYRVVAIVSGALNNAAAKKFDLESWFPFQSEYKELVSCSNCTDYQTRELEIRYGAKSQTSSKKTYAHALNATLCATERTLCCVLENYQTEHGINVPEVLRKYIPGQPEFLPYTKELPKDSISARGAKQSTSAELPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIT5</accession>
    <name>A0A8H6FIT5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Scavenger mRNA decapping enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HIT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156936.1">
        <property type="nucleotide sequence ID" value="XM_037298301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIT5"/>
    <dbReference type="GeneID" id="59335809"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000016">
        <property type="entry name" value="Scavenger mRNA decapping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.40">
        <property type="entry name" value="Scavenger mRNA decapping enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008594">
        <property type="entry name" value="DcpS/DCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011145">
        <property type="entry name" value="Scavenger_mRNA_decap_enz_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978:SF0">
        <property type="entry name" value="M7GPPPX DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05652">
        <property type="entry name" value="DcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028973">
        <property type="entry name" value="Scavenger_mRNA_decap_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102860">
        <property type="entry name" value="mRNA decapping enzyme DcpS N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR028973-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="36348" checksum="FDFDA39F8A4C42A8" modified="2022-01-19" version="1">MEAPTAADLVRHFRVDKLLNQDQGGRRVNLLGTINGSPALLTAERAAFPSNIDTLEAFHAAITSIDNLGENDIYRWYLASSGVTKEAPADLKINLIYPCTDKHIKKYSPQTVRMVTETPTIYQQHVRPYMQRMRDEGRLNWVFNIIEGRKEQEDILLRDPGQGSSREEAFLLTPDLNWDKKTLASLHLLVLVERRDFWSLRDLKKSHVDWIKHMRERILDATIELYPELEKDMLKLYIHYQPTYYHFHIHVIHVMCEATSTQAVGKAFGLENIISQLEAMAGGPEASMADVSLTYCLGDASDLWRNIFLPLKEGRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKA3</accession>
    <name>A0A8H6FKA3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157368.1">
        <property type="nucleotide sequence ID" value="XM_037295367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKA3"/>
    <dbReference type="GeneID" id="59332858"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="318" mass="35392" checksum="A6C61DC1092955CB" modified="2022-01-19" version="1">MPPKKAQTKDFVVDKSTEAGAKPAAESKEEPSTSKSNGTSKKRKAPAASGPNKVPRRSARSTPSAPVDVVKMLHFLLSPTSLDLCRPKDEIEDLQARGAETKTYSTSTFTPFEELVCAVILSRPIGHMLGLRSIRTIFNAPYEFTTPKKIREAGNEGCREALDKARTQHRQRTAEELVLLADAVVNSLGYGDEDVSLERVREESEHDYEREREMLRKNVKGLGKTGLDIFARRIQGVWPKVYPFVDQRTLAAAEKLGLPGSAEEMSDMLDEHWKKLDVKDIEAKDEEEKKRKAFVRILERAVGADLEGNIDSVKSEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKB1</accession>
    <name>A0A8H6FKB1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004391</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157309.1">
        <property type="nucleotide sequence ID" value="XM_037295308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKB1"/>
    <dbReference type="GeneID" id="59332799"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF15">
        <property type="entry name" value="UPF0136 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G03720)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="105" mass="10829" checksum="0EB112A5CC837159" modified="2022-01-19" version="1">MDDPATQAFLLALLTAGGGLTGYIRTGSIPSVAAGMTVGALYGLGGLRMRNRQPYGVEMALLASLVLAGSSVPRAMKTQKALPIGLSVVAAFGLYRFGTVWRAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKE2</accession>
    <name>A0A8H6FKE2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Origin recognition complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004490</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157408.1">
        <property type="nucleotide sequence ID" value="XM_037295407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKE2"/>
    <dbReference type="GeneID" id="59332898"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20704">
        <property type="entry name" value="Orc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020795">
        <property type="entry name" value="ORC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045667">
        <property type="entry name" value="ORC3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040855">
        <property type="entry name" value="ORC_WH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07034">
        <property type="entry name" value="ORC3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18137">
        <property type="entry name" value="WHD_ORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 3 N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 3 winged helix C-terminal" evidence="7">
        <location>
            <begin position="571"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6230151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="681" mass="76700" checksum="76A6974C97CE50F4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKF3</accession>
    <name>A0A8H6FKF3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004523</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157441.1">
        <property type="nucleotide sequence ID" value="XM_037295440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKF3"/>
    <dbReference type="GeneID" id="59332931"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="144" mass="16814" checksum="A00EB2765F04D3D9" modified="2022-01-19" version="1">MAEHDLFGPIADVPIAWSRSRALMTQKLLRVLPLLPALRFYLGKKVAFVYRAHREIRGTKIRVIWGKVTRSHGMLNRWVFYIVHLLNEVARQLWRCEGKLQKQSPTQIFRRLGQSDAVSVIDLAFQRVSKMDQKRRCGASCWPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKG9</accession>
    <name>A0A8H6FKG9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004553</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157471.1">
        <property type="nucleotide sequence ID" value="XM_037295470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKG9"/>
    <dbReference type="GeneID" id="59332961"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021838">
        <property type="entry name" value="DUF3431"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490:SF3">
        <property type="entry name" value="DUF3431 DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11913">
        <property type="entry name" value="DUF3431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034712909" evidence="2">
        <location>
            <begin position="29"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="404" mass="45972" checksum="648EFA04F3DB9055" modified="2022-01-19" version="1">MSALRKLIQLISTAAIIVLFSSLNKLNTEVSTLGKHRVGKHEELFLDASAWSLPGRNGSISETRDSLDSAATSTSSDFHPGVPKPLGSTYSRAMVIPRLQSDNISWVSDELPNVDAAVYTANDPLAVMHPPKNKGHEVMIYLSYIINHYDALPDILIFMHAHRWTHHNNALLAYDAAEMVRRLNSDYVTREGYVNMRCQWSPGCPEWLHPGNPRESLEKQEEVVLSKSWSELFPSDPLPAALGQTCCAQFALSKERVLAIPLSRYVYYRDWITRTPLSDYISGRIWEYSWQFLFTGQSIYCPAEHICYCGGFGLCFGGDSEFEEFKELRRKKRMFELETDELHERRALNGGTDMEIQSNNTSAVRYLYLNNQIDILEQELALRTKVALERGLNPRNRAEECGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKI8</accession>
    <name>A0A8H6FKI8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004526</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157444.1">
        <property type="nucleotide sequence ID" value="XM_037295443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKI8"/>
    <dbReference type="GeneID" id="59332934"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000014">
        <property type="entry name" value="SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="626"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="981"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="147"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="336"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="348"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="396"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6230187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1170" mass="129382" checksum="51C51B0110DCE77D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKR3</accession>
    <name>A0A8H6FKR3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004686</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157603.1">
        <property type="nucleotide sequence ID" value="XM_037295602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKR3"/>
    <dbReference type="GeneID" id="59333093"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF51">
        <property type="entry name" value="SNF2-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="467"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="372"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="667"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="693"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="802"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="852"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="874" mass="97907" checksum="F2BC4A6457BD70A5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZW9</accession>
    <name>A0A8H6KZW9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RuvB-like helicase</fullName>
            <ecNumber evidence="15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_004678</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA helicase participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RuvB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157595.1">
        <property type="nucleotide sequence ID" value="XM_037295594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZW9"/>
    <dbReference type="GeneID" id="59333085"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000001">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="63"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6230338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="456" mass="49709" checksum="54EB1295E8F4D6F7" modified="2022-01-19" version="1">MVTISEVKGNSREHRTAAHTHIKGLGLRSDGYAESASGGFVGQLAAREACGVVVDLIKSKKMSGRAVLLAGGPGTGKTALALAVSQELGTKVPFCPIVGSEVYSTEVKKTEALMENFRRAIGLRVRETKEVYEGEVTELTPEESDNPLGGYGKTISHLLIGLKSYKGTKKLRLDPSIYEAIQKERVVVGDVIYIEANTGACKRVGRSDAYATEFDLEAEEYVPIPKGEVHKKKEIVQDVTLHDLDIANARPQGGQDIMSMMGQLMKPKKTEITDKLREEINKVVSRYIDQGVAELVPGVLFIDEVHMLDIECFTYLNRALESPISPIVILASNRGKCTIRGTEDIVAAHGIPPDLLARILIVPTNTYTPGEVKLIVRLRTKTEGLAITEPALEKISEHGVKVSLRYALQLLTPANILARVNHKKEIDVDDVAECEDLFIDARRSASIVNAGDGFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5D6</accession>
    <name>A0A8H6C5D6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006866</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <begin position="68"/>
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        </source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="384" mass="42133" checksum="A61ED8040B1298FE" modified="2022-01-19" version="1">MSMGSSFHVENPHLGNKDRVEDWRIRGINPLTPPDLLQHEIAQTPRSKDTVLSGRNEVVSIVQNTDPNRRLLVIIGPCSIHDPAGALDYADRLMQLKEKYAGDLLIVMRSYLEKPRTTVGWKGLINDPDINNSFHINKGLRISRQLFVDLTEKGMPLASEMLDTISPQFLADLLSTGAIGARTTESQLHRELASGLSYPVGFKNGTDGTLDVAIDAIGAVMHPHHFLSVTKPGLVAIVGTMGNEDCFVILRGGKKGTNYDKKSIQEAKEALGKKGLRQRLMVDCSHGNSLKDHRNQPKVVNDLAGQIARGEEAIMGVMIESNINEGAQKVPKEGKEGLKYGVSITDACIGWDTTEEVLEILAKSVRKRREVLGMNGHQETNGHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6E8</accession>
    <name>A0A8H6C6E8_9LECA</name>
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        <submittedName>
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        </submittedName>
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        <name evidence="2" type="ORF">HO133_006577</name>
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        <name type="scientific">Letharia lupina</name>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="423" mass="47967" checksum="AB517D45CB07F4AF" modified="2022-01-19" version="1">MNSAFRSKYAALLNEDWVKLGYQSEHAEHGLGLTNQVHPIFQRTQALRPRGAEHIWPQYKSEGEYDAMCAEMGTVFQLASKMLESPKSLEFLYQVAYSPRKVSNVGFGNQGRPCKEFGWTDPPFTGRQMARNALRHLSRSLTFEIGDPEADPEVQGSFAVTAATLVHFGAGVAIDDVSRKTGMASRITLNGSWILKLKELDAQEGDTTSQKLSLQVKMAITLCHEIAHALAFAVNREYLEMSIRHYNAADQAHKEGRAYEYPVQELRTDEPYYRKETMAEIGNSWENQVFGGKINWSGNLSDPLFVSKWPSFLTSDDDYCPTRGKEKRTARKYVVSLHFIRNMHRQGFWDNVRSEHTNALYIKKTVGIEYGNPDTEAISVNSSEINWPHDEENSSRVSREKDGQTIPDPSDSRANETAEERRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7E0</accession>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6218340.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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            <end position="1034"/>
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        <location>
            <begin position="1108"/>
            <end position="1147"/>
        </location>
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            <begin position="1209"/>
            <end position="1221"/>
        </location>
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        <location>
            <begin position="1281"/>
            <end position="1296"/>
        </location>
    </feature>
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        <location>
            <begin position="1323"/>
            <end position="1340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6218340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1420" mass="153554" checksum="DB8E889A27640F11" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7E4</accession>
    <name>A0A8H6C7E4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
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            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147654.1">
        <property type="nucleotide sequence ID" value="XM_037297080.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59334582"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="512" mass="57343" checksum="7C348E57D09A5762" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7F5</accession>
    <name>A0A8H6C7F5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxymuconolactone decarboxylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006207</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147680.1">
        <property type="nucleotide sequence ID" value="XM_037297106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7F5"/>
    <dbReference type="GeneID" id="59334608"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052999">
        <property type="entry name" value="PTS1_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180">
        <property type="entry name" value="CONSERVED MITOCHONDRIAL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180:SF5">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="27269" checksum="EE324F3CCA6382CA" modified="2022-01-19" version="1">MSSSEEPTADEAKDLFEKLEQKFPSKTLGAERWYLVAIAALTGGGQPEFAGNLYAYLVERPHYATSEARQALVRRLREALVKSVSIIGVCRPLEAIFNIDAVQKPEDKDYSFSRENWQSGPENHARGVAWLDKIYKHNRTGTINSLAAHKDFAWISANITYGLYLSDRQILDDIDTEMVVLSGIMIQNLKRETGWHLRGTRRVGVSHEDVEVVQQCIEMVAAFARVRLNKIPRVADIEHEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7Q0</accession>
    <name>A0A8H6C7Q0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147641.1">
        <property type="nucleotide sequence ID" value="XM_037297067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7Q0"/>
    <dbReference type="GeneID" id="59334569"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="480" mass="52918" checksum="0E98C77B5C9049FE" modified="2022-01-19" version="1">MPRRFRQPFNNRNKHEMMDTFSPRSATLMLRPVQETFVNLALIVDGVGWPRHDGSNPDLTSCRALRWGLVSSSFFLESAKPSEDRSGVWALMPRNIEEIIIEFSSKGGLSHFADRLHTPNFGNPWYNETMFSDGSWLVEIAAQKTEHYPNLESIVMSDLDIVKGTVSKLRSLEDVQTGPYHIYNCGTRTPSVSVLINTLIATLQPVLDDVSRPFSSPAYTTFFKNIAFAPIVYDLLSNITTGAPTSPGPHALKDAPPELFGAPITPQFVCVTAYEQLTWSLEPGGQGGRQLDAYTACHQSPVHAFATVGTRFLKNYIVLCPAFWTYAAIPSSSKSTCLPVDPHFNRFRDGGGRLVQFQLWVILHELAHAYIYARSGLVTDVSTANNCMWLAASSAVNNAQNFVFYAANIRLGCTDFPRVARPSFGSVELLEIDANTTLSGGSEPQNMSMYGGQSIGNNSTRTNMDLPNIVAPAPYGQPSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8G5</accession>
    <name>A0A8H6C8G5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148235.1">
        <property type="nucleotide sequence ID" value="XM_037296253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8G5"/>
    <dbReference type="GeneID" id="59333749"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="45598" checksum="AAF78AC421FDFC08" modified="2022-01-19" version="1">MFLLDLPPELLALIADCLGASELRKSVAYLLLAKRWYQAVLPVYLSQLPLSDLYLASHHDLERLPPSDTALSNLIQTKAKRLSVRFVGHPSIRPSVVPWHDNGDLYHSDNLGLDGTKRLKNWHCDWMTVGPVERYSGRRRWHWHREQETLHQWGYRINKKLVELAAMMFNSKCLEEFSLEASSEHTDEHGPRWDYLHESTIRSLISSLPSSLNNLTLDLCGSGTITPDRGIPTAHLCPLIGQRLRDFRNVRLRLRCICPQVLRSSSLIPNVGSRLKTLIIKLSLPSFPEAADEVHNGYNEFDAKSCLVTAIPLYKRMIAAGVDSAKNSPELSLMRISYRSQSDTDDRSSYNLAVADCVRKQYMFEPTEKFCYEDDGSQWDAWEDSDTLRDGGPFEELLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8R1</accession>
    <name>A0A8H6C8R1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005478</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148370.1">
        <property type="nucleotide sequence ID" value="XM_037296388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8R1"/>
    <dbReference type="GeneID" id="59333884"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032156">
        <property type="term" value="C:septin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000238">
        <property type="entry name" value="Cell division control 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Septin-type G" evidence="8">
        <location>
            <begin position="24"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="425"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="467"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="314"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="672" mass="75336" checksum="A85CF24641879B46" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8S7</accession>
    <name>A0A8H6C8S7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-1,2-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005509</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148400.1">
        <property type="nucleotide sequence ID" value="XM_037296418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8S7"/>
    <dbReference type="GeneID" id="59333915"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF7">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034995915" description="Alpha-1,2-mannosyltransferase" evidence="5">
        <location>
            <begin position="26"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="46795" checksum="9773DF0FF2C866F9" modified="2022-01-19" version="1">MAIPRPLRALAIVSAFLLFYLLFQAFRTPPTIHGQGDLEHELPNEPTLQEYGEPPPPLWRADGYAADNPDSPRINATLLALVRNEELDGMLVSMRDLERTWNSKFNYPWTFFNDVPFSEDFKRLTQMETKAKCNYELIPKEHWAVPSWINSDLFDESAKMLEEQEVQYGPMVSYHQMCRWNSGMFYKHPALQDVRYYWRVEPKVHFFCDIDYDIFRWMNDHNKTYGFTINLYDAPQTIPTLWPETEKFMAAHPEYLHENNAREWVVDSKRRPEHNIKAHGYSTCHFWSNFEIADMGFWRSKPYEDYFEHLDRAGGFFYERWGDAPVHSIALGLFEDKSKIHWFRDIGYQHIPYFNCPKSPKCRGCVTGRFTDGERFLFKEDCRPNWFKYVGMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8T1</accession>
    <name>A0A8H6C8T1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">A-pheromone processing metallopeptidase Ste23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005591</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148482.1">
        <property type="nucleotide sequence ID" value="XM_037296500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8T1"/>
    <dbReference type="GeneID" id="59333997"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000003">
        <property type="entry name" value="Insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000005">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000004">
        <property type="entry name" value="Putative insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="7">
        <location>
            <begin position="33"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="8">
        <location>
            <begin position="210"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="9">
        <location>
            <begin position="395"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="10">
        <location>
            <begin position="790"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6219047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1095" mass="123977" checksum="4F94B978B3C24F36" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Y9</accession>
    <name>A0A8H6C9Y9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dihydrolipoyllysine-residue succinyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_005136</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148746.1">
        <property type="nucleotide sequence ID" value="XM_037296048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Y9"/>
    <dbReference type="GeneID" id="59333542"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000006">
        <property type="entry name" value="Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004309">
        <property type="entry name" value="PRK05704.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="14">
        <location>
            <begin position="23"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="96"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="168"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6219311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="432" mass="47479" checksum="9D00329FDA1345D5" modified="2022-01-19" version="1">MVDFVVVLTRCQTQYAEAKKASDKVVKVPEMAESISEGTLKQWSKQIGDYVEQDEEIATIETDKIDVAVNAPEPGTIKEFLANEEDTVTVGQDLLKLELGGPPKGGEKQQGSQKPKAPAPDEQSTSSDPEPQKDEGKSEQESPPPPPSSEKESEPSKQESKPSPPPKEPSKHKEPSPKPQESKKPEPKSKDTAPLGNREERRVKMNRMRLRISERLKQSQNTAASLTTFNEVDMSNLMQFRKLYKDEVLKKTGVKLGFMSAFSRACILAMRDIPAVNASIEGPGGGDTIVYRDYVDISVAVATEKGLVTPVVRNTETLDLVGIEKEIAELGKKARDNKLTIEDMAGGTFTISNGGVFGSLMGTPIINLPQTAVLGLHAIKEKPVAVDGKVEIRPMMYLALTYDHRLLDGREAVVFLVKVKEYIEDPRRMLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA47</accession>
    <name>A0A8H6CA47_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005227</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148836.1">
        <property type="nucleotide sequence ID" value="XM_037296138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA47"/>
    <dbReference type="GeneID" id="59333633"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000159">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF39">
        <property type="entry name" value="PEROXISOMAL MEMBRANE ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="2"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="157" mass="16410" checksum="E3F4D06445613B0C" modified="2022-01-19" version="1">MVKAGDSIPNVDLVEGSPGDKVNLSKELASGKGLIIGVPAAFSPSCSATHIPGYIGSDKLKNAGKVFVVSVNDPFVMKAWGATLDPTSKTDVRFLGDPSGQFTKDLDLSFESAAVFGQDRSKRYALVVEDGKVKSAHVEPDNTGVNESAAEKVLGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA89</accession>
    <name>A0A8H6CA89_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148961.1">
        <property type="nucleotide sequence ID" value="XM_037294914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA89"/>
    <dbReference type="GeneID" id="59332404"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="504" mass="55191" checksum="9872B01B90C18E3C" modified="2022-01-19" version="1">MAKREEDESFELQQNRWTELINLRQAMIDYKMDPAGVELAISNVFDPKKGRIIESMILDGQKLMATILRLEELQIDPSNVKMTLEKAFVPGMNGAYDSDLLRVKGMIEMREQMAAMGLPTGCVEVSISEVFKTVKSEEVSEASATKPFSPTAGIIQPVELDASPIAIFNQSTAEIGKPAELDASETAITMQSSSSTEAGKPVESPARLTEMRPVSKDTETDKPAEVPSQPTSMKHPSSDVEHIAIIGTSIKAMDLDSSPATTSSPLAKAPSVSTSPSTVRSAPSAKTASSLNRPSTPRPTLSAKRSAPTDTEIELGSAQNPICLGSSPAPPKRQRTTNDVASRADPKTRESWKIPIESVGIMGLHLKPRYDDADLSIAFDVVTQRVVFFKGQQSLTALYPGLEIVPEALLSVTRPLYTQSKQLRVRFMWLDTDAEESFLDITIATKQDCRVLIARLKNLTSFSEILLPEPRMNRLYEDATWVVNETPNAFGVSVTKFSENVWLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAL4</accession>
    <name>A0A8H6CAL4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149453.1">
        <property type="nucleotide sequence ID" value="XM_037294765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAL4"/>
    <dbReference type="GeneID" id="59332254"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="33188" checksum="DB2E1D1CBBC7CF42" modified="2022-01-19" version="1">MDPETSSFKSPIRQLTDNNYSEWLIDVKALLRGKKLWNYTQEAPDPDKSVTAQAKWRDSAMEAADLITPTISGPVKQKLQANAFDDGYLMMTSLVNIYAPKGDAEFMRLTREYYSLRYEDFDSMTHYLTQIKTLEERIRGTNVILDDDKQTLLCLGMTLPERFQYFTKIWAVTLGMTADKARSMLLEEERREKTLDTGGVGLVATTRHPGKRMPSGKAILNYEHEVKCSRCGKPHEDEVCWKLHPELAPEWLQERWTIEKRSRKRKWDELQQDQEAPGEKEAFINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAU1</accession>
    <name>A0A8H6CAU1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149558.1">
        <property type="nucleotide sequence ID" value="XM_037294177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAU1"/>
    <dbReference type="GeneID" id="59331665"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="262" mass="29712" checksum="3764F21998D34F59" modified="2022-01-19" version="1">MQQGSQTTPRVKSVSKTGLQGVKAWEVRCFQWRKAKRLPHWEGEDSKNNGLSKRREQQGHITEGERRREIPFKTGQRQRKDLFLRKLRESEEVEKGKGNNRKAQPTKAIGLRRRAFLNQEFSLTNSTPTPQDLVIDPNDVYQVPEERNRYQSQTLQRRRHQHSNGPTAPPPPPAAALEPTAHFSTSRSTRTAGKAAWTGSKGLVVGLHDEVRDENGDGFWDAKDARLSSGWRGEAGSEEWECLKLKVGGRGWVGSGWIGRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBN2</accession>
    <name>A0A8H6CBN2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_002867</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF2B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149870.1">
        <property type="nucleotide sequence ID" value="XM_037293792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBN2"/>
    <dbReference type="GeneID" id="59331279"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000002">
        <property type="entry name" value="Translation initiation factor 2, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6220435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="328" mass="36746" checksum="FEABE3E7B3FC8237" modified="2022-01-19" version="1">MSLTNCRFYENRYPDIDEFVMVNVKQIAEMGAYVKLLEYDNIDGMILLSELSRRRIRSIQKLIRVGRNEVVVVLRVDKEKGYIDLSKRRVSPEDIVKCEERFNKGKMVASIMRHVAEKTKTPMETLYQDIGWPLNKKYGHAVDAFKLSITNPDVWAEVTFPSDVVKTELQSYISKRLTPQPTKVRADVEVTCFGYEGIDAVKNALRAAEAQNTATHTPSKTADGLDIPRPNEATVKVKLVSPPLYVLTSQCLEKSTGIACLERAIKEIEGNIRANGGGCVTRMAPKAVTENDDAELQALMEKRERENAEVSGDESISESDEGVVAETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBR2</accession>
    <name>A0A8H6CBR2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002835</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149838.1">
        <property type="nucleotide sequence ID" value="XM_037293760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBR2"/>
    <dbReference type="GeneID" id="59331247"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF20">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="354"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="39"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="544"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="556"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6220403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="568" mass="61902" checksum="7B000589059761B5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBY3</accession>
    <name>A0A8H6CBY3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003033</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Microtubule binding protein that promotes the stabilization of dynamic microtubules. Required for mitotic spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLASP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150035.1">
        <property type="nucleotide sequence ID" value="XM_037293957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBY3"/>
    <dbReference type="GeneID" id="59331445"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060172">
        <property type="term" value="P:astral microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="9">
        <location>
            <begin position="2"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="9">
        <location>
            <begin position="280"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="559"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="736"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="595"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="754"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1167" mass="129427" checksum="5E320DC0368E640D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC71</accession>
    <name>A0A8H6CC71_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150041.1">
        <property type="nucleotide sequence ID" value="XM_037293963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC71"/>
    <dbReference type="GeneID" id="59331451"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34286">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34286:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="110" mass="12200" checksum="6E1A0568854DA5A6" modified="2022-01-19" version="1">MEKDHSIKSVESKGASISHSSGGKIPYPKHVWSPAGGWYAQPANWKANTAVMGVVVIGITAIAWNISAEREIRPRMPEKGRFFPSRYWSRQIIEHERAEAAKEAAAKGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC87</accession>
    <name>A0A8H6CC87_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002462</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150217.1">
        <property type="nucleotide sequence ID" value="XM_037293388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC87"/>
    <dbReference type="GeneID" id="59330875"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF11">
        <property type="entry name" value="OBG-LIKE ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="21"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="306"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="30"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="394" mass="44051" checksum="3985CCCB26E856EF" modified="2022-01-19" version="1">MPPKKKEEEKKVLLGRPGNSLKSGIVGLANVGKSTLFQAITKCSLGNPANFPYATIEPEEARVIVPDDRYDWLCEQYKPKSKVPANLTVYDIAGLTRGASTGAGLGNAFLSHIRAVDAIFQVVRCFDDAEIIHVEGDVDPVRDLTIISEELRIKDIEFVEKALENLKKQTRRGGQSLEMKKLKEEEATVEKILAWLKDGKDVRKENWTPKEVEVINPLFLLSAKPVVYLVNLSEKDYIRQKNKHLPKIAEWVKEHAPGDPILPLSVAFEERLTRFETEAEAEEECKKLGTKSALPKIITTMRKMLDLGSFFTTGTDEVRQWTIRNGTKAPQAAGVIHGDFEKTFIQCIVYNYEMLKEYKDETSLKGAGKVMTKGKDYVVADGDIILIKAGAAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCN9</accession>
    <name>A0A8H6CCN9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150259.1">
        <property type="nucleotide sequence ID" value="XM_037293430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCN9"/>
    <dbReference type="GeneID" id="59330917"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.10">
        <property type="entry name" value="Trypsin Inhibitor V, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021719">
        <property type="entry name" value="Prot_inh_I78"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39600">
        <property type="entry name" value="PEPTIDASE INHIBITOR I78 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39600:SF1">
        <property type="entry name" value="PEPTIDASE INHIBITOR I78 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11720">
        <property type="entry name" value="Inhibitor_I78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="83" mass="9255" checksum="B2C34A4B7F028B3F" modified="2022-01-19" version="1">MPLVVPGLMSSGGSNNQQEDWMNKLVGKKLSDSTSDTTSFAKQDLPKEHRVIEHGSMVTQDFKPDRLNVHLAEDGTVRQVDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD62</accession>
    <name>A0A8H6CD62_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Charged multivesicular body protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002286</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150865.1">
        <property type="nucleotide sequence ID" value="XM_037293212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD62"/>
    <dbReference type="GeneID" id="59330699"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF12">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="178"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24048" checksum="C591FF0CB4CFE676" modified="2022-01-19" version="1">MNRLFGAKNPGPKPTLSSAISTTDSHVESLDVKLAKLNAELTTYQQRMSKMRDGPGKTALKQKALKILQRRKMYEGQRDQMQQQSWNMEQASMMTDNLKNVMTTVDVMKSTNKSLRKQYGKVDIDKIERMQDEMQDLMEVGNEIQESMSRSYDVPEDVDEAELDAELEALGEEAEFEGGLGMGQGVGEMPGFMQDEVPSFIDEPPEGAKVKEAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD98</accession>
    <name>A0A8H6CD98_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase SSN3</fullName>
            <ecNumber evidence="6">2.7.11.22</ecNumber>
            <ecNumber evidence="5">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Cyclin-dependent kinase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Serine/threonine-protein kinase ssn3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">HO133_002149</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the SRB8-11 complex, a regulatory module of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="6"/>
    <dbReference type="EC" id="2.7.11.23" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150729.1">
        <property type="nucleotide sequence ID" value="XM_037293076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD98"/>
    <dbReference type="GeneID" id="59330563"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07842">
        <property type="entry name" value="STKc_CDK8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000408">
        <property type="entry name" value="Serine/threonine-protein kinase SSN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000426">
        <property type="entry name" value="Serine/threonine-protein kinase ssn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF495">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0010">Activator</keyword>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="90"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="437"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KAF6221294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="477" mass="53718" checksum="C1860683DDFD0FD9" modified="2022-01-19" version="1">MSPCSNNPSIPPTFLRNHFDNGITPNHDDPISNSPEPDTPESDTTQPAYLDPYYLEPYSHNFVLPTESSEARKMPPTNGYTSKVRVLDRYKVVGFISSGTYGRVYKAVGHNGMAGEFAIKKFKPDKEGEMIQYTGISQSACREMALCSELSHPNMIHLVEIILEDKCIFMVFEYAEHDLLQIIQHHSQPTRHPIPASAVRSILFQLLNGLLYLHSNWVLHRDLKPANIMVTSKGEVRIGDLGLARLFYKPLHSLFSGDKVVVTIWYRAPELLLGSKHYTPAVDLWAVGCIFAELLSLRPIFKGEEAKMDSKKTVPFQKNQMQKIVEILGMPTKDKWPGLIHQGEHSQLQTLSSYVREGNNGKSGLESWYWNTLRSTGYQDSPPSVTPGQQGLDLLSKLLEYDPMKRLSAEQALQHPYFTANGEKVSANCFEGMSTQYPSRRVSQDDNDIRTSSLPGTKRNGFPDDSLAGRPTKRMKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDF7</accession>
    <name>A0A8H6CDF7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002254</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150834.1">
        <property type="nucleotide sequence ID" value="XM_037293181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDF7"/>
    <dbReference type="GeneID" id="59330668"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="137" mass="14832" checksum="1E66EB6F81D9934E" modified="2022-01-19" version="1">MVALGGNPPITANVGSNDPTSGGKASRASQTCDHKCAVTTLSIAIPILSLFIIGFLVFGLSHRRRKMRKNKAKDQETEIAIRKLGPESETSSVTGFGDFPAVTNVEDGAEAVRVSEKPSSSWKSRKLFLKTPGWWRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDH1</accession>
    <name>A0A8H6CDH1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Polyadenylate-binding protein</fullName>
            <shortName evidence="14">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_002250</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation. In the nucleus, involved in both mRNA cleavage and polyadenylation. Is also required for efficient mRNA export to the cytoplasm. Acts in concert with a poly(A)-specific nuclease (PAN) to affect poly(A) tail shortening, which may occur concomitantly with either nucleocytoplasmic mRNA transport or translational initiation. In the cytoplasm, stimulates translation initiation and regulates mRNA decay through translation termination-coupled poly(A) shortening, probably mediated by PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150830.1">
        <property type="nucleotide sequence ID" value="XM_037293177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDH1"/>
    <dbReference type="GeneID" id="59330664"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000355">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000384">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
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        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 13 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="66"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="154"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="247"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="351"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="17">
        <location>
            <begin position="705"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="381"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="643"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="783"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="382"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="670"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="783"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6221395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="810" mass="87140" checksum="0062CA388B321A2A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDJ3</accession>
    <name>A0A8H6CDJ3_9LECA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150998.1">
        <property type="nucleotide sequence ID" value="XM_037293345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDJ3"/>
    <dbReference type="GeneID" id="59330832"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="160" mass="16970" checksum="5DFD254CBF7CF77E" modified="2022-01-19" version="1">MTDNGTFPDGNKSLTLIGGFAIGLALAENVKSGAESAEDLETIVRRFGSDNLPRYIGEKVGLVQVLVSDYMCSAKVAEAEEVTMIMFVRTILDALYLDTGMSGVKSSLGKCDGVLGKRSKSEPGADAAMGYLDVFDEPYGEDEGEGKRTRADIVEICGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEF8</accession>
    <name>A0A8H6CEF8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037292863.1"/>
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    <dbReference type="GeneID" id="59330349"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="539"/>
        </location>
    </feature>
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        <location>
            <begin position="655"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="683" mass="72696" checksum="524AFEA55EA918ED" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEW5</accession>
    <name>A0A8H6CEW5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protection of telomeres protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001186</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the telombin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151535.1">
        <property type="nucleotide sequence ID" value="XM_037292116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEW5"/>
    <dbReference type="GeneID" id="59329602"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000783">
        <property type="term" value="C:nuclear telomere cap complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098505">
        <property type="term" value="F:G-rich strand telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032210">
        <property type="term" value="P:regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04497">
        <property type="entry name" value="hPOT1_OB1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000303">
        <property type="entry name" value="Protection of telomeres protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028389">
        <property type="entry name" value="POT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032042">
        <property type="entry name" value="POT1PC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011564">
        <property type="entry name" value="Telomer_end-bd_POT1/Cdc13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513">
        <property type="entry name" value="PROTECTION OF TELOMERES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513:SF0">
        <property type="entry name" value="PROTECTION OF TELOMERES PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02765">
        <property type="entry name" value="POT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16686">
        <property type="entry name" value="POT1PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00976">
        <property type="entry name" value="Telo_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomeric single stranded DNA binding POT1/Cdc13" evidence="10">
        <location>
            <begin position="9"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="359"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="633"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="639"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="649"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="683" mass="77172" checksum="823E14F88CDF0A42" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF84</accession>
    <name>A0A8H6CF84_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GS catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151549.1">
        <property type="nucleotide sequence ID" value="XM_037292130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF84"/>
    <dbReference type="GeneID" id="59329616"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43383:SF2">
        <property type="entry name" value="AMIDOHYDROLASE 2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43383">
        <property type="entry name" value="NODULIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS catalytic" evidence="3">
        <location>
            <begin position="524"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="849" mass="95444" checksum="D76197840CAEF9BE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFR5</accession>
    <name>A0A8H6CFR5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151819.1">
        <property type="nucleotide sequence ID" value="XM_037292400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFR5"/>
    <dbReference type="GeneID" id="59329886"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043041">
        <property type="term" value="P:amino acid activation for nonribosomal peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45527:SF1">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45527">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="321" mass="36363" checksum="EECF90E4E12A2C73" modified="2022-01-19" version="1">MLQTVFEMRVSFNQKLLRQTRQQQQNKNQILRTRHVKHKDRILRVVVNDTIQRESGYNLAKYKASDMSKRVGSGDPLFRYAIVSEGDRSFLVWTCHQGGFDGYKALGCSQFSRTIEIKKAGGTNSFVTLPTLGHAAWALAVGSLWSVDDVLFGSVKMGRQMARDSPLCRVESGKGPMMAVQDELLSTIQYKHEGWASTMDCLGPQTIRPGMIDWDPLGGEVFSRTLKHRAPKGELGYLKPRRDLAVHFTINASMLVDIYEHEHHLNLRVSYDANMWEEKLVTKPVDTFTNLLTKMLFSRDGKVGELLLGGAEGMTRLRDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFX7</accession>
    <name>A0A8H6CFX7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trichothecene 3-O-acetyltransferase-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000904</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152199.1">
        <property type="nucleotide sequence ID" value="XM_037291837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFX7"/>
    <dbReference type="GeneID" id="59329322"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054710">
        <property type="entry name" value="Tri101-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF64">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22664">
        <property type="entry name" value="TRI-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Trichothecene 3-O-acetyltransferase-like N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="453" mass="50575" checksum="BEA29056E7CED792" modified="2022-01-19" version="1">MEKYEDLDRYQDILGQLPMLQCYSHILYMFPMPEGVTREEVIRDLEEAVTKVRDKVPWMGSRVINVGKKPGNSGLYRVIACPRPENPIDVKEVSNALPPYAIIKERKAPISVIASELLTPVSAFPQMFEDSDEDPAHVVRLQASFIEGGLILDFALQHNMGDAGGHFGFVKLVAMAMRGEEFPDSLLEQANRDRRNLFLLLSPDEPMLDHSHHKRPPIAADAPLVRPEPARYHILRFTAAKMGKLKELASSPEGLDPDVPFISTDDALSAFCWQRYTTVRSHRFPPETKSRFSRAIDGRRLVGVAPDYMGDVIHNASTWLSFQELTESPLSTIASHLRKRLNGTNSAYHVRSFATFIAREADKSTITYGGPFNPDTDVGCSSVRGRTDLYPGFGTLGKPDFIRRPPTVPFPSLLVFFPGDSGAYCDASACLTDADLEALNADPEWNKYVEYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG23</accession>
    <name>A0A8H6CG23_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MAT auxiliary protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000964</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152259.1">
        <property type="nucleotide sequence ID" value="XM_037291897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG23"/>
    <dbReference type="GeneID" id="59329382"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="40342" checksum="53B36087EE4DE302" modified="2022-01-19" version="1">MSFRNNLADRAFQRSTTDDNGIESSMSSFGGARIRISNEPELRRRIKELDEGGWEWRFLAYAEEDKGLLESSSQAGSHCSYAYLEAIRHNLGIAKMACQLEMDAEYELIEDVIMATKSELSRLQEHPELFPSQDIREKFWHSVRLKCNESVKFVISEQEARDHAHEMFQAVSVEVEENTRTPGRDMQRTPWEQFLHNRAPGAEADLSMFAEEGSVQIIPMAAARILMATLEEHAAIIRDIEAQREDLKPYAPSSSSHLHFCGLKLSPDGHLRLRKVSNDLCKTTPAGPLDVGRHEDVNSLETLNLDFAALNQRRPPLRPRMWNCVTVVVHDRPNYGVDSEQRDETVEEMTSMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGD8</accession>
    <name>A0A8H6CGD8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001086</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152380.1">
        <property type="nucleotide sequence ID" value="XM_037292018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGD8"/>
    <dbReference type="GeneID" id="59329504"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="408"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="167"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="359"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="379"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="784" mass="89129" checksum="211C9A93B66D2824" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGF8</accession>
    <name>A0A8H6CGF8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glyoxal oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000792</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152090.1">
        <property type="nucleotide sequence ID" value="XM_037291728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGF8"/>
    <dbReference type="GeneID" id="59329210"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02851">
        <property type="entry name" value="E_set_GO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
        <property type="entry name" value="Galactose oxidase/kelch, beta-propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009880">
        <property type="entry name" value="Glyoxal_oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015202">
        <property type="entry name" value="GO-like_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208:SF21">
        <property type="entry name" value="LOW QUALITY PROTEIN: ALDEHYDE OXIDASE GLOX-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07250">
        <property type="entry name" value="Glyoxal_oxid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09118">
        <property type="entry name" value="GO-like_E_set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034536370" description="Glyoxal oxidase" evidence="4">
        <location>
            <begin position="19"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Glyoxal oxidase N-terminal" evidence="5">
        <location>
            <begin position="262"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose oxidase-like Early set" evidence="6">
        <location>
            <begin position="634"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="737" mass="77802" checksum="B339EADAE0E443A9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGT4</accession>
    <name>A0A8H6CGT4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Checkpoint protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000104</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the HUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152479.1">
        <property type="nucleotide sequence ID" value="XM_037291044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGT4"/>
    <dbReference type="GeneID" id="59328523"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044778">
        <property type="term" value="P:meiotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016580">
        <property type="entry name" value="HUS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007150">
        <property type="entry name" value="HUS1/Mec3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900:SF0">
        <property type="entry name" value="CHECKPOINT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900">
        <property type="entry name" value="MITOTIC AND DNA DAMAGE CHECKPOINT PROTEIN HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04005">
        <property type="entry name" value="Hus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011312">
        <property type="entry name" value="Cell_cycle_HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR011312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="355" mass="38946" checksum="5D4356BC7CB110DF" modified="2022-01-19" version="1">MRFKASIRNIHTFTRLTASLSLLGKDVWLQLNNEQLRFTVVPEQGSQVWAVLAIDTIFETYTIQSAVENNTINLRIPLASLHRALRSALTASSASIRLTKKDNIPLLSLTIITNNLNSSRPPTSITTTTLNSDNAPFPVDDAYNNNESLGDPNPGFFPHDRETTITQDIPVMVLAAATVASIHEPQCPQPDVHIMLPSLLQLKAISERFTKLALSTSTSATNRGRASDALSSQSRLTLSANAHGVLRIGVETPSLKIESRWEGLTNPELDPEQVEGGEEGVRGHASTRMKEREGDEAWSVVRVEGRDWGRVLGVGRLGGRVIACFCHEHALILYVYLSGDDTESVLTYYIASYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGZ7</accession>
    <name>A0A8H6CGZ7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152612.1">
        <property type="nucleotide sequence ID" value="XM_037291177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGZ7"/>
    <dbReference type="GeneID" id="59328656"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="229" mass="26948" checksum="E32D05A2E8FF0B4F" modified="2022-01-19" version="1">MTYETVVKMKWLRRTWESEGWSSRAYELDLEKNIELEIRELIGKDVVNFDDFIENNLAIKNDVYNHGDSISAVHHRVSYKHGRLPTLRAARQLQRASGSSRFEFDRVFEAFERARFSKFSFNFEHHTDFKTFSDVVGAAEAKQSFRFQVHPFEPTWSIVQHKTRPQSRRKWIGAFAWLPVMHKFRSILLHNSPVTHQHSLHNLQTAPKASQPLAVTHPPQHFHFNNQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGZ8</accession>
    <name>A0A8H6CGZ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152390.1">
        <property type="nucleotide sequence ID" value="XM_037292028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGZ8"/>
    <dbReference type="GeneID" id="59329514"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="154" mass="16316" checksum="475F1F971CBEFACB" modified="2022-01-19" version="1">MLPSQRADGLRAGKTKRSPSPSPPPGRGDESPFISHGPVLPTENRAAASSEPQNTVYGGTRAPETIPSTTVTCSPRPQPQPIGVVPTEGIHHGTQPAGDNDAYPESTIPAYYRAPHRGPRRTRSGQSSRPGVDFIVRRKPLDPVNSTVGPSNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIC1</accession>
    <name>A0A8H6CIC1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010598</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153084.1">
        <property type="nucleotide sequence ID" value="XM_037301450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIC1"/>
    <dbReference type="GeneID" id="59338988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF31">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN YCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="6"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="233" mass="25125" checksum="CC89558EB2520013" modified="2022-01-19" version="1">MAEDVEKILPALENVTDFILAGHGMGAKIAQIVASGEPKGLIGLALINPVPASPWILSSPKMERLYGSCEAKTEALDFANGVLSAHPGSLTVSDLDKISAHAIKQDTALTIAWLREGSEGKEGDWSGAVDKIKVPAMAIAGKSDRLISVQTIKDEVYGKIYGCIMVEVEDAGHLLPLERPKKLLEELQAFVDACVDVRKNDGLRSGSLLYQGFCVARESNYNNFMRTQGLESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIG2</accession>
    <name>A0A8H6CIG2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mur ligase central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010661</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153147.1">
        <property type="nucleotide sequence ID" value="XM_037301513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIG2"/>
    <dbReference type="GeneID" id="59339051"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000010">
        <property type="entry name" value="Dihydrofolate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase central" evidence="7">
        <location>
            <begin position="25"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="416" mass="45082" checksum="4E89BF6B112B124F" modified="2022-01-19" version="1">MIELGLARITRLLKNTPIPWRAIHVAGTNGKGSVCAYASAMLNAGNINCGRFTSPHLIDRWDCITINEKSVDEDFFREVEAAVKTKDQSEDIQASEFEILTATAFEIFNREKIEIGVVEVGLGGRNDATNVLEHPAVTVVTKIGKDHQSFLGSTFEEIAMHKAGIMKAGVPCIADGSNTTGVLEVLKSNAVEVGAASLLLVPQDADPAGQVWNVVAKEELMDHQQMNICLAFEAVKEVLSHCYPSLDPLRLLPSIPNAVWPGRLQNLTIKSLAGNERQMLLDGAHNNQSVEVLGTYVDQKLREKHRPVTWMIAMSKGKDIQELLYFLLRSGDNLIAVEFGPVEGMPWVCPEEADSILGAARGLGISDSSQILSGDLAQALQVAVRIADGGPLIAAGSLYLVSDILRLLKQAELLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIW3</accession>
    <name>A0A8H6CIW3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010837</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153322.1">
        <property type="nucleotide sequence ID" value="XM_037301688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIW3"/>
    <dbReference type="GeneID" id="59339227"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="167" mass="19239" checksum="A22818C3E433E61C" modified="2022-01-19" version="1">MRIPWDGSSMRVMEVALSDRPPSIFTDGRTEELEADLVHLPKLWDCVSRHSFRWEFRTLLALLTHTDLRTITQSMRDRMVTPDNFLYTCLEAGSGLRLNGYLSHLCGVRVYGDAFIFRWDPQQYGPDGRATLIDTEEIFIESEYNGTFLPKLLCNFFKPGEGKGIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIW6</accession>
    <name>A0A8H6CIW6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Conidiophore development protein hymA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mo25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153399.1">
        <property type="nucleotide sequence ID" value="XM_037301765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIW6"/>
    <dbReference type="GeneID" id="59339306"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000257">
        <property type="entry name" value="Conidiophore development protein hymA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013878">
        <property type="entry name" value="Mo25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182:SF3">
        <property type="entry name" value="PROTEIN MO25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08569">
        <property type="entry name" value="Mo25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="42399" checksum="8886ED3B1E43BCE4" modified="2022-01-19" version="1">MLESSPMAFFWGRNKPKSDLVKPAKDLLNRLRQPPKLQKTEEEVAKLLAQMKLVLTGTQETDSNPESVLQLITSMIQEDFLYVLACSIHLLSFESRKDSQAIFSYVLRFRPPGSTAEDSPALSYVINDRPEVVIELCRGYNNKESAMPCGAVLREVLKHELIVSIILYDQSLENQLDTKIDDIAYLDVPQSGDGVFWKFFDWIDGGAFEVSADAFTTFRDILTKHKPQVSQYLMTNFDLFFKRYNTILVESSSYVTKRQSIKLLGEILLDRANYNVMTAYVDRGDHLKLCMNLLRDDRKMVQYEGFHVFKVFVANPHKSDAVKRILTQNRERLLRFLPGFLADRTEDEQFTDEKSFLIRQIETLPPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK38</accession>
    <name>A0A8H6CK38_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Peroxisomal hydratase-dehydrogenase-epimerase</fullName>
            <ecNumber evidence="5">1.1.1.n12</ecNumber>
            <ecNumber evidence="6">4.2.1.119</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Multifunctional beta-oxidation protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_010056</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Second trifunctional enzyme acting on the beta-oxidation pathway for fatty acids, possessing hydratase-dehydrogenase-epimerase activities. Converts trans-2-enoyl-CoA via D-3-hydroxyacyl-CoA to 3-ketoacyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a (3R)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32711"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57319"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.n12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a (3R)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:26526"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57319"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="EC" id="4.2.1.119"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.n12" evidence="5"/>
    <dbReference type="EC" id="4.2.1.119" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153729.1">
        <property type="nucleotide sequence ID" value="XM_037300912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK38"/>
    <dbReference type="GeneID" id="59338448"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018812">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03448">
        <property type="entry name" value="HDE_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05353">
        <property type="entry name" value="hydroxyacyl-CoA-like_DH_SDR_c-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000410">
        <property type="entry name" value="Peroxisomal multifunctional beta-oxidation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000013">
        <property type="entry name" value="Peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000185">
        <property type="entry name" value="peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054357">
        <property type="entry name" value="MFE-2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051687">
        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22622">
        <property type="entry name" value="MFE-2_hydrat-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ketoreductase" evidence="22">
        <location>
            <begin position="314"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="758"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAF6224862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="903" mass="97569" checksum="DDE7850EB6222473" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKB9</accession>
    <name>A0A8H6CKB9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein-S-isoprenylcysteine O-methyltransferase</fullName>
            <ecNumber evidence="3 10">2.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_010233</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="2.1.1.100"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="3 10"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153905.1">
        <property type="nucleotide sequence ID" value="XM_037301088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKB9"/>
    <dbReference type="GeneID" id="59338625"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025770">
        <property type="entry name" value="PPMT_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF9">
        <property type="entry name" value="PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51564">
        <property type="entry name" value="SAM_ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6225038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="148" mass="16940" checksum="B6693CC4D71E1683" modified="2022-01-19" version="1">MAFVECFLHYHYIPKLTRSIFPPAVHTAWLVLGAAMLAIGQGTRTTAMVHAGSNFNHLIQSKKKEGHVLVTGGVYRYLRHPSYFGFFWWGLGTQVVLGNVVCLVGYAVVLCRFFRRRIEKEETLLVGFFGIDYVRYRDRTRVGIPFIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKD4</accession>
    <name>A0A8H6CKD4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153864.1">
        <property type="nucleotide sequence ID" value="XM_037301047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKD4"/>
    <dbReference type="GeneID" id="59338584"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034979822" evidence="2">
        <location>
            <begin position="30"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="396" mass="43184" checksum="4575CF369CD76B38" modified="2022-01-19" version="1">MHVPFLGRCLWQALSISYLLTTYARGVHAYPQTNLTSPNSNTSLALSSPILLPSRFSSLTLTYGELSPDRTYTPEPTFTIRPKTRVARYDPGAKCPPALLHLCLPTLASCLGEKALTPEFRVGFTPADIALAFVAALAQQAVEPFDARLSYFEYADAETPVRIVLRSAPHAPTTLTVRSNVMWALKQLPINLFHDPSIWGLDFKVRLRGEDLYLGSLSNSNRPGGVLGAGGEQGGGNGSSDGSEEKQKRALLITQPSNTTAQNLKNPGSSPTDIQYDLRFQLTGLPLPDIGIFSAILEFLLGLGAGDPYQAVESAKLALDTLPVWIYISYNAQPSSTQRLQVYRVVAILQAIARYCVGQRIYQELIFNLLVDDLLLASGCVVRGVGEREWCRGLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKI4</accession>
    <name>A0A8H6CKI4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial phosphate carrier protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_010267</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transport of phosphate groups from the cytosol to the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153939.1">
        <property type="nucleotide sequence ID" value="XM_037301122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKI4"/>
    <dbReference type="GeneID" id="59338659"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035434">
        <property type="term" value="P:copper ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000076">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000131">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF13">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PIC2, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12080)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="27"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="121"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="222"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6225072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="34029" checksum="2E2813215C4D5FF9" modified="2022-01-19" version="1">MATEKAATKMPGRSEKGKGGIELYSPTYFGACALGGIISCGPTHTSVTPLDLVKCRRQVDSKIYSSNVSAWRTIYKAEGLRGVFFGWSPTAVGYSFQGAGKYGFYEVFKYTYGDKLFPNANKTVVYLAASASAEFLADLALCPFEAIKVRMQTSIPPFASTLREGWGKVTAKEGTGGLYKGLYPLWGRQIPYTMVKFATFETTVNYIYATLGKPKETYNSLQQTGVSFLGGYIAGIGCAVISHPADVMVSKLNADRQAGEGAGKAMSRIYGNIGFKGLWNGLPVRIAMIGTLTAFQWLIYDSFKVVLGLPTTGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKU1</accession>
    <name>A0A8H6CKU1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010236</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153908.1">
        <property type="nucleotide sequence ID" value="XM_037301091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKU1"/>
    <dbReference type="GeneID" id="59338628"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="215" mass="23448" checksum="E24286028E6D34BE" modified="2022-01-19" version="1">MDEKVPENSVQSEYTQVPRERLAPGTVANFPGSLVANHLGGTLKATTYPQDKGRLANWAAYFREGWDDPGIWKSAFVEAFASFLLCYLSGMIDKTITNFGTSQAPAYAGVVNIFLLTLFIMAFAPGSGGQFEQSGPHQSHHHILNNDRGFDGFLTRYFLIFHTRHRKERLPSASAILYMSGQLLGAGLAGGVLRGAFGADRSIESVMSKSSAPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKV2</accession>
    <name>A0A8H6CKV2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153981.1">
        <property type="nucleotide sequence ID" value="XM_037301164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKV2"/>
    <dbReference type="GeneID" id="59338701"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="223" mass="24445" checksum="4FE77BC2452EA85B" modified="2022-01-19" version="1">MPSIKSPSTEASVPTLYDETQAPDMSSTKLPSNADSLSTLYDETHRGIWHINPHIASFGNSPCLIAHLEHHGPTTDGSFAVCVAGGSGNFINKKLYESIPQEHRPELDTCPHRVGLMVGEDQTALGTAFIPIILKNADTDKPFRIVLHAYVLPNMLMGMFISDPAWVEVQRFRPSGREYGCDFGDGKISAVRVLFLCVARVLQNVALTDAKYIGVLKDFESKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CME4</accession>
    <name>A0A8H6CME4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA excision repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009186</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154873.1">
        <property type="nucleotide sequence ID" value="XM_037300047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CME4"/>
    <dbReference type="GeneID" id="59337581"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000019">
        <property type="entry name" value="DNA excision repair protein ERCC-6-like 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029256">
        <property type="entry name" value="Heliccase-ass-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057931">
        <property type="entry name" value="RHH_ERCC6L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25806">
        <property type="entry name" value="RHH_ERCC6L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14773">
        <property type="entry name" value="VIGSSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="232"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="602"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="34"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="807"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="897"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="922"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6226320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1053" mass="117763" checksum="12BBB72D3A0121DC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMF9</accession>
    <name>A0A8H6CMF9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DUF221-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009828</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154535.1">
        <property type="nucleotide sequence ID" value="XM_037300687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMF9"/>
    <dbReference type="GeneID" id="59338223"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF149">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G11660)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="441"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="486"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="602"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="656"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="681"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="40"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="215"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="392"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="850"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="865"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6225826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="968" mass="108916" checksum="9B23CF0D2FC84AA2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMT1</accession>
    <name>A0A8H6CMT1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008971</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154659.1">
        <property type="nucleotide sequence ID" value="XM_037299833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMT1"/>
    <dbReference type="GeneID" id="59337366"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="175" mass="18880" checksum="2941AA4BC11FE7CF" modified="2022-01-19" version="1">MEMALPLMGRVFSHQVAEQQVNREESSMEPGVSMSKGLVFNGSTLLWSSMLEDTAGLRSAIIPGEATTPDDDIFEYEENIGASLILGSAVPLPLQKVLFQYLPPRQEADRLTAAYFGILVTGANTGMGFEMVRALCNSEVAYEVPIGGSSLVKAGQAANAVMKEFPSTRSRVFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNL2</accession>
    <name>A0A8H6CNL2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_009405</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterohexamer; composed of 3 copies of TIM9 and 3 copies of TIM10, named soluble 70 kDa complex. Associates directly with the TIM22 complex, whose core is composed of TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Peripheral membrane protein</topology>
            <orientation evidence="1 15">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155092.1">
        <property type="nucleotide sequence ID" value="XM_037300266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNL2"/>
    <dbReference type="GeneID" id="59337800"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038:SF16">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="16">
        <location>
            <begin position="17"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6226539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="98" mass="10462" checksum="C78E1ACF1ABD7E50" modified="2022-01-19" version="1">MSFLFGGRPQLSSAEKIAAAETEVEMVSDMFNRLSQSCTAKCIPPEYREPDLNKGESVCLDRCVSKFFEVNLKVSEKMQGEAAQKGGAGGGGMGGFGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNS1</accession>
    <name>A0A8H6CNS1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155378.1">
        <property type="nucleotide sequence ID" value="XM_037299377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNS1"/>
    <dbReference type="GeneID" id="59336907"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="331" mass="36775" checksum="D802E89E3992E853" modified="2022-01-19" version="1">MSNLIDPKTGSNGTPSKGLEQNRSTGEELRMDANVASDGSEEGEIQDDHATNAVGTKRKRSPSTEETGPSKKTKGPDPTFLSFKTMRHPTETIPCVISINEREPFGSLYLTNHKLTTRLFLDPGRFGPPSVALTFTKTGSETGRGIAKNVWDMSSNSRVEWAMNDVQHGYATSDGPDLRMSNRTVLADCSRRDIQNLMYLRFKSWSQVSGFHKKEEFSQESAEIQKSIKTMFRRQKAYQLELWFIAPFDAPDFRRHCLSTSGFSFGLEAPTSIPSLISSPRPGFVPDFTLGPLDIEDEESDSDSEYDSESGSHSDWERKHEGQRGGDGSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP97</accession>
    <name>A0A8H6CP97_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SMP-30/Gluconolactonase/LRE-like region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008491</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155358.1">
        <property type="nucleotide sequence ID" value="XM_037299357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP97"/>
    <dbReference type="GeneID" id="59336887"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="4">
        <location>
            <begin position="20"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6227050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="308" mass="34645" checksum="EE6F227FEA71F3D4" modified="2022-01-19" version="1">MAEMQRWTVTEPYIKMSCGLGEAPYYESSRHTLRFVDIVKEKLHIVDLNKGPSSLESFDLGTPVSTTADIEGSDDEIIVGAKHGYAIMNRNTKKLEYIKKVWEERDGLGKDERMRFNDGVVDHEGRYWASTMNDPKVKAPPTDEGVLFRLDPDMTLHRMIEKVSIPNGICFSPDAKIMYFIDSPTSNVFKFEYDRKTGDISNREVFYHVEGDAVPDGMTIDVNGCLWIAICGGGKVLKVSPEAKVVGEILLPYARMITCPAFAEEDLFITSAEEEEPDKYPDSVKYGGSLFKVHVGVNGLGVHNFKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPH1</accession>
    <name>A0A8H6CPH1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155140.1">
        <property type="nucleotide sequence ID" value="XM_037299139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPH1"/>
    <dbReference type="GeneID" id="59336669"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021822">
        <property type="entry name" value="DUF3405"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36205">
        <property type="entry name" value="CHROMOSOME 19, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36205:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11885">
        <property type="entry name" value="DUF3405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034970473" evidence="1">
        <location>
            <begin position="33"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="658" mass="75472" checksum="D0870F085ADE1D82" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPN3</accession>
    <name>A0A8H6CPN3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155344.1">
        <property type="nucleotide sequence ID" value="XM_037299343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPN3"/>
    <dbReference type="GeneID" id="59336873"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02208">
        <property type="entry name" value="cupin_RmlC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="2">
        <location>
            <begin position="40"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="127" mass="13682" checksum="8897AE904CD8C6D8" modified="2022-01-19" version="1">MAAPVQVTKASELHTNTGQTEGMVRQGAIIDKSDSISASVMIAKPRSSSAIHHHGPQDTVVYCVRGRGAVVSEGGKKKQVLEKGDFALIPAHAEHQEINDEDEEVEWIITRSGRVPVVENLEGWGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPR9</accession>
    <name>A0A8H6CPR9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SprT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155304.1">
        <property type="nucleotide sequence ID" value="XM_037299303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPR9"/>
    <dbReference type="GeneID" id="59336833"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006640">
        <property type="entry name" value="SprT-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10263">
        <property type="entry name" value="SprT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SprT-like" evidence="1">
        <location>
            <begin position="135"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="268" mass="30743" checksum="A3F5B2245984707C" modified="2022-01-19" version="1">MAVVTHRDRDDLYDEAFEDVHTFSGTPYPCTQDELDVSGGVNRQEPISTLTIFEALRRNGPIEEHLYPGLRDLWFDHRNLGPFISARTAAREVHDHNNLSRGPPGAERQAIDRLDVAIDDAYTHKWGPDLIIKAFCDLDTVFFRGRLRGHVCVAWKPDHLMGQSKYGVTGYLGHGKCTINLNAAEILRYQSSSKAFEKMWSTMLHEMCHAYAQVRVREHEWRDDERLHGKNFGTKIHAVARRADYLFGLFVDINQFGSDVPFPRYDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ38</accession>
    <name>A0A8H6CQ38_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA2/NAM7 helicase helicase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008868</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155732.1">
        <property type="nucleotide sequence ID" value="XM_037299731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ38"/>
    <dbReference type="GeneID" id="59337263"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF16">
        <property type="entry name" value="HELICASE WITH ZINC FINGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="7">
        <location>
            <begin position="267"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="42391" checksum="8B354153AA435876" modified="2022-01-19" version="1">MTDKKYAMALKETKLNEEPLTGAKQGPPGTGKTRLIGFCVLNCVQQQQRWLLTAETHYAVQVCADRIYANLQAVDGNYNGIWLIERADLDSAAFTVSSSDFPDDQAKISELTRTKIIQRLRGMRSPNGSLLPLTSQIASDFSETNKECEPTRLQFLEISMYHNWPSTTLVVKRMSFDKVWEDTQEYYIRTQALGIIVTAATAMTKMLRVFRPQQIIVNEASQLTEFATVAVVSRFFENLRKVLIVGDPCQKKPFVLNQNSEFVNTTETSLMVRLSNSGVPITRLREQFRMHPHIAQPVSSLSYEATLINSPSVLSRPKDRIWQTFHGQTLQECAKRHSIFVHVKNYVPYRLKKTQSMLKPSRLAIVRALIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQG8</accession>
    <name>A0A8H6CQG8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155919.1">
        <property type="nucleotide sequence ID" value="XM_037298583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQG8"/>
    <dbReference type="GeneID" id="59336110"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="175" mass="19567" checksum="6C6D5BBFFFA7099A" modified="2022-01-19" version="1">MRVAVELPLFEAVCATDGDSITAREIAKSQDVKEDLVGTLAPLRHEKRERMPKICSLYNARPHGSGCLRRAGLCNYSPNDVTKEFTSRGLVDGVKCLSLKRTGPRVPKPPEETALAYADKATLFEWMKKAPEQRKYFDSFMTGRRKGIQTPKKGPGSPPRGDQKNAILRHFEYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQY0</accession>
    <name>A0A8H6CQY0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155793.1">
        <property type="nucleotide sequence ID" value="XM_037298457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQY0"/>
    <dbReference type="GeneID" id="59335984"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="140" mass="16038" checksum="D1D2A41D33FC904F" modified="2022-01-19" version="1">MDGWVNSVLNTGVYKTGFATEQGNHEKACIEVFEALDQVGDILANSKGEYLFGEHFTEADVRLYPTIVRFDTAYYTLFKCNLKIIRHDYPKIQRWLVNLYRQKGFGDTTDFEHINKGYALTPRNNLVLLGPAPDMLLAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR94</accession>
    <name>A0A8H6CR94_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007841</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156047.1">
        <property type="nucleotide sequence ID" value="XM_037298711.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336238"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.130.10">
        <property type="entry name" value="Ubiquitin-protein ligase E3A, N-terminal zinc-binding domain (AZUL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032353">
        <property type="entry name" value="AZUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042556">
        <property type="entry name" value="AZUL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF8">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16558">
        <property type="entry name" value="AZUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="7">
        <location>
            <begin position="883"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="116"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="361"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1153"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="375"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="1312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1344" mass="150641" checksum="998FCC09F7D32EB6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRK1</accession>
    <name>A0A8H6CRK1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155875.1">
        <property type="nucleotide sequence ID" value="XM_037298539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRK1"/>
    <dbReference type="GeneID" id="59336066"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021838">
        <property type="entry name" value="DUF3431"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490:SF1">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11913">
        <property type="entry name" value="DUF3431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="314" mass="35478" checksum="9AF97F01BD07EADA" modified="2022-01-19" version="1">MSLDLSKTASPAPASATLFTPESSSPTSSPTDISPALTKASSSTTLSSPSIEFVVAHYSEKLYWLKDVIAYCKIYSKGGPKNAPDFAFEELDNNGREGQTYLYYICKYYDELKDITVFTQGKAFDHTGMTALEMMDNALGTKVGQITTFGTQRDLELFDLWDGIPWKDYPCWQKWLGKNLEFIGKKAPQTPAQYLKDFFDNYGHAGPPMSVGFQPGAIFAVRRETIHRHPKKTYQKMLADLFEGDMVHNDPETGHFMERLWLATFCPEEYCCWTEAEVSMVERNQQGQLAKGRWHRTPPGVQVDEGHTNLSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS96</accession>
    <name>A0A8H6CS96_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156350.1">
        <property type="nucleotide sequence ID" value="XM_037299014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS96"/>
    <dbReference type="GeneID" id="59336543"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="303" mass="33787" checksum="273030EA2A1B7DE5" modified="2022-01-19" version="1">MRRFAATIAAAVVPPSDGINQTGFHYNLTNAARLGDIDPRFTTDYRQGQARLSATSCLMNAANAMMELALENFTESIRPRDYTDPGYPEVVVVPMTMAPGQGIEARYLLWGVWKGIRWMISHQSFRDLVIGAYWDGILMCRIWMKGRQLSIAGSNDTLGLTARSEKMSIRNATVEFTQDLSKLDVRNPLNDQHLTVSVTQVGEMLGITEVFVAIFATLEYIAQFPSTDRVVGFQISPDDEDTTIGFLGHPRAPAVRTPSLEYQWVILSVGQIPGYLLQQRRFTEVIIEIAVDGVPLGEGFMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSM1</accession>
    <name>A0A8H6CSM1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006946</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156477.1">
        <property type="nucleotide sequence ID" value="XM_037297842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSM1"/>
    <dbReference type="GeneID" id="59335346"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="144" mass="15987" checksum="11968E519413B63F" modified="2022-01-19" version="1">MSFGFSTGDILAVSKLAHDIWVRGLYIVLDDAAQSLPGPKLDQKQMSRLASLVQACQGVHDDTNAVLEEFESLGVKSSSLKVMTQKARKKITWDEEGTEVLRGRIISNTMFLNAFSKMDFSIEFLLAEKCNPGSTTRRNREMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CT04</accession>
    <name>A0A8H6CT04_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156476.1">
        <property type="nucleotide sequence ID" value="XM_037297841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CT04"/>
    <dbReference type="GeneID" id="59335345"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="331" mass="37421" checksum="A9876724743C9A95" modified="2022-01-19" version="1">MDALAEIWSRSGQRSEDLYKGTTDEIIKELKAWIVARNVRNETNMRLLWDFFDTNDAISGLQNQSEYLDDLRIAAFKAKHVKSVERVKEFKEERDKLTLRASYLLSTLSHRGLKWTGMPCTPAWTEMDSEVCAFLKTKDRRFDAEKQLEILEMEHEIAEWRLEHARREAVEAARLVGVGNTNDQNATPRAHRVALVGSGGQEDGPRAPASYSLPALVDVTGLLPRPRPRAEDNSENPLLPGPMDTIEEELNEEETAEAIAREIDNMETTEEPAKVGKKSKAKIRDARRKKARVRAMETAKAERASKDDVVDKELSDQMSKASVSSPKNCDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F766</accession>
    <name>A0A8H6F766_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006277</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147300.1">
        <property type="nucleotide sequence ID" value="XM_037297175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F766"/>
    <dbReference type="GeneID" id="59334678"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000006">
        <property type="entry name" value="NOL1/NOP2/Sun domain family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049561">
        <property type="entry name" value="NSUN5_7_fdxn-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048889">
        <property type="entry name" value="NSUN5_RCM1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF4">
        <property type="entry name" value="28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21148">
        <property type="entry name" value="NSUN5_fdxn-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21153">
        <property type="entry name" value="NSUN5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="6">
        <location>
            <begin position="152"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="261"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6217865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="550" mass="60756" checksum="C74B9729AB2DD11C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F825</accession>
    <name>A0A8H6F825_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148157.1">
        <property type="nucleotide sequence ID" value="XM_037296175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F825"/>
    <dbReference type="GeneID" id="59333670"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="158" mass="18145" checksum="6BF97EFD3B7D45E2" modified="2022-01-19" version="1">MYEPHRPLEQRSKGQERQKQKGKWWSHPNEREEQERKQARQPVEHTDDVYDVIDEETERSNTNYPKAKAKPKMPGAAAEEKPSRHWPRISVTNMKSTGEYSKPSTVEGKDMRAEGKQFLRKVKSAVNLFGEETAASSAETAPEVPRIPTSSKNPPKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8B7</accession>
    <name>A0A8H6F8B7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UDP-N-acetylglucosamine transferase subunit ALG13</fullName>
            <ecNumber evidence="2 7">2.4.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">Asparagine-linked glycosylation protein 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ALG13</name>
        <name evidence="10" type="ORF">HO133_005827</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 7">Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UDP-N-acetyl-alpha-D-glucosamine = an N,N'-diacetylchitobiosyl-diphospho-di-trans,poly-cis-dolichol + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23380"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19510"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57269"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.4.1.141"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 7">Heterodimer with ALG14 to form a functional enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.141" evidence="2 7"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8B7"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043541">
        <property type="term" value="C:UDP-N-acetylglucosamine transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004577">
        <property type="term" value="F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052474">
        <property type="entry name" value="UDP-GlcNAc_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47043">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE TRANSFERASE SUBUNIT ALG13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47043:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE TRANSFERASE SUBUNIT ALG13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="9">
        <location>
            <begin position="11"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6218478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="201" mass="21770" checksum="698E9402FF7F4EED" modified="2022-01-19" version="1">MPRKKRKSQKVCFVTVGATASFDSLVKAALSPQFLEALETHDYTDLRLQHGKDGQKLLEGFRRSSYAMSEEVQDLSISGFDFNKQGLGSEMRAAKGQGNGVEGVVISHAGSGSILDALRIAVPIIVVPNSDLLDNHQEELAEELAIQGYVVHGNLHDLPAAIRQSESLRKTQNPWPPADTGEDPNGKGLVGVMDDEMGFVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8F2</accession>
    <name>A0A8H6F8F2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148246.1">
        <property type="nucleotide sequence ID" value="XM_037296264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8F2"/>
    <dbReference type="GeneID" id="59333760"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="827" mass="90491" checksum="277BD87003C6F672" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8F8</accession>
    <name>A0A8H6F8F8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148562.1">
        <property type="nucleotide sequence ID" value="XM_037295864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8F8"/>
    <dbReference type="GeneID" id="59333358"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="80" mass="8993" checksum="4E89C43F9EC78AB6" modified="2022-01-19" version="1">MPISKKDRINREHKKADAAGTRAPVKANGNPVKAPTPMSKCAYCMKEIVASNQTQKKVHAETHDQKVWTKEKCWPDDTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8I3</accession>
    <name>A0A8H6F8I3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_005027</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148637.1">
        <property type="nucleotide sequence ID" value="XM_037295939.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333433"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009403">
        <property type="term" value="P:toxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05930">
        <property type="entry name" value="A_NRPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19532">
        <property type="entry name" value="C_PKS-NRPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF20">
        <property type="entry name" value="HYBRID PKS-NRPS SYNTHETASE APDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="11">
        <location>
            <begin position="10"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="12">
        <location>
            <begin position="1015"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="10">
        <location>
            <begin position="2490"/>
            <end position="2565"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="10">
        <location>
            <begin position="3659"/>
            <end position="3738"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="8">
        <location>
            <begin position="1015"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="8">
        <location>
            <begin position="1168"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2573"/>
            <end position="2642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2574"/>
            <end position="2587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="2588"/>
            <end position="2627"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="8">
        <location>
            <position position="1047"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="8">
        <location>
            <position position="1231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6219202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="4092" mass="447765" checksum="06347C092E9337DF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8T5</accession>
    <name>A0A8H6F8T5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148289.1">
        <property type="nucleotide sequence ID" value="XM_037296307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8T5"/>
    <dbReference type="GeneID" id="59333803"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="72"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="231" mass="26763" checksum="D70AFD4C1D6AB22B" modified="2022-01-19" version="1">MASKSPVPLDSDGFPVVTSPITGNRRGYTEIVHPWCGGTTLREVLLQGGSALEDARKLAKETQLVKKILEARRRSEIRAATETSEQRQERLRRHALVQNPLADEQRGEDSAKQSEYYRNKPRVKPVTEKQRPRKRETASDRKRRETASSTKERKRKQNEYMRKRYAANPKTDEEYYRKRLAAKPRTKEQRREDSARAKKKRAAKMLLLHDSMVPAFRRHHDTNAMSPSIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F915</accession>
    <name>A0A8H6F915_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148529.1">
        <property type="nucleotide sequence ID" value="XM_037295831.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59333325"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
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        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="612" mass="66721" checksum="5608499E0F1EE90A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F998</accession>
    <name>A0A8H6F998_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003919</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148888.1">
        <property type="nucleotide sequence ID" value="XM_037294841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F998"/>
    <dbReference type="GeneID" id="59332330"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="99" mass="10955" checksum="0C0FF7AE6E89086D" modified="2022-01-19" version="1">MATRPFCIRLLHVALYDRGVQDANATLPTLHDTTDPITSGTTPHNLNTGFAKTDKDLWRFESKSIPQTGPSLLLVRITIIEVVTKVSYSIFGGWGTLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9C8</accession>
    <name>A0A8H6F9C8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149552.1">
        <property type="nucleotide sequence ID" value="XM_037294171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9C8"/>
    <dbReference type="GeneID" id="59331659"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="140" mass="15063" checksum="3B9F48DBD63AE860" modified="2022-01-19" version="1">MWVNGDDSFTASTDAALPDNAFPFARLASVTLADQSTTFLYHQINGTTFAEEQWDASPGAWIAAIYLPRLQHPNAVSDDANQLPTAGLFDNVEPNMMLQDDDELEAGCSRASEKDDESSLGDYVESQPSSDIQANAGTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9N0</accession>
    <name>A0A8H6F9N0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HD/PDEase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003579</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149189.1">
        <property type="nucleotide sequence ID" value="XM_037294501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9N0"/>
    <dbReference type="GeneID" id="59331990"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.50">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594:SF1">
        <property type="entry name" value="HD_PDEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G03035)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="2">
        <location>
            <begin position="187"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="480" mass="52976" checksum="13963B03D2C06094" modified="2022-01-19" version="1">MPRALSTALRARIKADLAAGHDIKTIMATHAISDKKARAMKKLYDNCGEVWLPKKKGARTGRPPKITPDHEERLRAYLADRPDAYIKDMCKFLEGACGMLVDESTMWRTVRRLGWQAQRVVKPRDARGFWVQTLPRDESGNVVTNAQRRKPTKRREPSMDPSRAMVKQLMAKTRGFVRDCMARAPFDVAHDYAHVRRVLALSVEIQRVEQKKYRHITFDATVTELVALMHDIDDHKFRQPPPTQNHASLPSPPSNLDPQYPNPSPIDPNLHLPPPPLQPPIPPTPAVESHLINLGWPPHIAITVSIICASISYTTETQHPDIHLSALRRFPELAIVQDADRLDALGAIGIGRTFAYGGARGRDLGDTIRHFEEKLVKLEGMMKTGEGKRMARAKSERISMFKEWWGEEMGMVGIGVPGGGVGVGDDGIAFSGAEMGDGGMGGETARRASDEDPGRQLMEANDGSDDDGESSGEGTSSDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9Q8</accession>
    <name>A0A8H6F9Q8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003669</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149279.1">
        <property type="nucleotide sequence ID" value="XM_037294591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9Q8"/>
    <dbReference type="GeneID" id="59332080"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021863">
        <property type="entry name" value="FAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11960">
        <property type="entry name" value="DUF3474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase N-terminal" evidence="9">
        <location>
            <begin position="97"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="8">
        <location>
            <begin position="161"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="505" mass="55493" checksum="F6C4DAC156F412A1" modified="2022-01-19" version="1">MSTVAFSRKSVLQTPVVESLPSSSSNSISATPDRTPSQSHSSTSLSSLASDSEVDVQKSAGNLVNNKQSSDLVDTKLSGQSVTSEAQYGRLVDTYGNEFQVPEFTINDVRAAIPKHCFERSAARGLMYVARDIFSLATTFALFYLLWTPANVPSDSVRAAGWALYTFIQGCFGTGLWVMAHECGHQSFSPSKTLNDTVGFICHSALLVPYFSWKISHGKHHKATGHLERDMVFVPKTREQYASRIGRAAHELAELAEETPIVTAGSLVGQQLAGWFLYVSINRTGHNSHTRQKEGRGQGKKNGWGGNVNHFDPSSPLYEAKDSKLILASDLGLAAAAGILILIGKNFGWANLAVWYFLPYLWVNHWLVAITYLQHTDPSLPHYEDAAWNFARGAVATVDREFGFIGRTLMHGIVETHVLHHFVSTIPFYNADEASEAIKPILGRHYRSNVEGGPWGFVKAMWTSARTCQWVEPSEGAEGLGKSILFFRNRNGNGLPPAKMTPLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAT6</accession>
    <name>A0A8H6FAT6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase II beta 2 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150404.1">
        <property type="nucleotide sequence ID" value="XM_037293575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAT6"/>
    <dbReference type="GeneID" id="59331062"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038816">
        <property type="entry name" value="Stationary_phase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42342">
        <property type="entry name" value="STATIONARY PHASE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42342:SF1">
        <property type="entry name" value="STATIONARY PHASE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="51573" checksum="07C9398B37C62B61" modified="2022-01-19" version="1">MAPSSGHLLVPKVLRIVKFAIDKTSSIIRAKLPQSRIANNASLQPIRIYAQNQPVHPLAYLRQSRSQNSNRWFSTTAHQFTTSAAKARTGVKYDRSTFPISKVSKAISQRGSAPFASTLRPNLTGGALPRCVGGYSLGGAGKGARHFSHTTGVHAQVVHNVSAGIRAFFVGGGKARFDGVNPVTGEKRFRSVSQTEDAVYSQWESPMSSSMKGTNLEFKFSPTITALCPSFSSEKSNLDVTSLNDIDLLSTLTSDFARAIQDLSLILTDLRALSALGDLPISLIHTAAGPILSVRFPGCDADLVSRLCDEVGVRRGVVVEDEGWHTAPVGQAGQAEKDVEMALLFPFAPNATPSLPSEGGEYYFTNATQRQRQPEQVDWRNMLSPSQQSQYTAESLHDNHSTEDRTLKSPDRHAHSPISGYESLGDSDFASEDPYFHTASPVKAATTHGTRAESGEYEGLEGIYKFLQVCEEAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB51</accession>
    <name>A0A8H6FB51_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151066.1">
        <property type="nucleotide sequence ID" value="XM_037292527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB51"/>
    <dbReference type="GeneID" id="59330013"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="422" mass="45750" checksum="0DDA6C48A2133C08" modified="2022-01-19" version="1">MSPNAMVSVDTSEKRAALNPNHANIEPNTSSLDPFSTQEMGVQEPESSNSSGPPPSTRNTPLENPFRDGDAPVGYFYPNSSGMQEGLNPATATQMDPAPANLPPVLTANADPFADADASAAEMTLDESLLPHPSLPARPSGLSRGLQIPSRISLITWGFGFPQILAEQGVTKSQWKLFMRELKAFAELSVGQWVTVVACTHGVSLLFGAIISFDVVNVESGHIAGHKMMKHRQHENFLLAYKGGAMKVFEHRWNNDYFHPLGLQIRIEPPGIGKVDGMDITSSKLFRYQQKMGTSSPAPGIASKQGDKKEYKYQSKEGRYRMKAARKGRIIVLPLNMVKPVPSQPRTTGSESHNGQTEGSATSARPSVQDFACSSQRQSGSEGLDRTPTTAQRLATSHQRQSSSDNAPSPARAPTTHWGGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCT9</accession>
    <name>A0A8H6FCT9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000468</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152842.1">
        <property type="nucleotide sequence ID" value="XM_037291407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCT9"/>
    <dbReference type="GeneID" id="59328887"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002070">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR002070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="112" mass="12349" checksum="94C607E1819E5EA2" modified="2022-01-19" version="1">MIIVGRLADAPEIVATSRGQDMIRYALATNHGPKDNRQTSWWKVASFLPEGGARDLLMALGKGSLVYVEGDCSMNKFQNKDGIASSALNIVQRNFEILDRRDPRAEGQIEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD86</accession>
    <name>A0A8H6FD86_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Low temperature viability protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152796.1">
        <property type="nucleotide sequence ID" value="XM_037291361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD86"/>
    <dbReference type="GeneID" id="59328841"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04180">
        <property type="entry name" value="LTV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="310"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="362"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="410"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="376"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="49026" checksum="AA52417BAB1CC692" modified="2022-01-19" version="1">MPRKRWIDKNSSTTFQLVNRPQNDPKIHDASSSSMLFQELAPSQAHKIKNRNDLEAELFGPTESSILSGVRDNEGEAAEHGIFYDDTEYDYMQHIRDLNAGSGSGESYFVEAATKKKDGKGKGKMSLEDALRETSLQEERSESVASQSGGSQLLDDELLPSKDLRRSTYQDQQDVPDALAGFQPDMDPRLREVLEALEDEAYVDDEEDFFGEIAEDGREVSLEEFEEAVFEDAGDEAGWETDETEKPSKEYETGDMPPPPVPQSTDTMMEDGPDHGDGDWMKEFSKFKQTDKAAKKAQLQVSNAELQSSIMTGTSMTGGRRKKRKGAMTSSTGYSMTSSSIFRTEGQTLLDARFDKIEEAYAEDEMDMDDGGASIMTGSSMASSQAPSLVQRADFDSIMDDFLGGYSMSGKKRVKKGGYQSGMEQLDEVRKGLGPARVKPQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDU5</accession>
    <name>A0A8H6FDU5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Non-repetitive nucleoporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_010036</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the non-repetitive/WGA-negative nucleoporin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153709.1">
        <property type="nucleotide sequence ID" value="XM_037300892.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338428"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051292">
        <property type="term" value="P:nuclear pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1780:FF:000003">
        <property type="entry name" value="Non-repetitive nucleoporin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.440:FF:000001">
        <property type="entry name" value="Nuclear pore complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.450:FF:000002">
        <property type="entry name" value="Putative non-repetitive nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1880">
        <property type="entry name" value="Nucleoporin, helical C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.440">
        <property type="entry name" value="Nucleoporin, helical domain, central subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.450">
        <property type="entry name" value="Nucleoporin, helical domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007187">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014908">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004870">
        <property type="entry name" value="Nucleoporin_Nup155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042533">
        <property type="entry name" value="Nucleoporin_Nup155_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042537">
        <property type="entry name" value="Nucleoporin_Nup155_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042538">
        <property type="entry name" value="Nucleoporin_Nup155_C_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03177">
        <property type="entry name" value="Nucleoporin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08801">
        <property type="entry name" value="Nucleoporin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like N-terminal" evidence="14">
        <location>
            <begin position="117"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like C-terminal" evidence="13">
        <location>
            <begin position="683"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="34"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="437"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6224842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1374" mass="151810" checksum="C89E6C007AD82F4D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDV7</accession>
    <name>A0A8H6FDV7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetraspanin Tsp3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010829</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153314.1">
        <property type="nucleotide sequence ID" value="XM_037301680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDV7"/>
    <dbReference type="GeneID" id="59339219"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="32204" checksum="0D46248FB2F728E2" modified="2022-01-19" version="1">MPSSSQALSFLVPVLLVGLVVTGGIALYRIRAFSLPISTVTATATIILPVITGVTLRGAQRLRIRENGSNARAKMSFSWPALIVLVLLIIYETVIATLALTHVAPPSELICGLERQWGALFSSKNADAIRRIQEQFQCCGFRNVQDRAWPFPDRSHTARACVEAFGRGSSCLGGWRQMEQVTGGLILLVAVVAFLLKLLMLVLYRTQSPFLSSAWTARSSLTDAEGEDNGLSSGNGDGDNIRGRIVDAYHDEPPPEIEGQAASSQPEERDTSASDRRGLLVQPSGIQNERNEWREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE56</accession>
    <name>A0A8H6FE56_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153902.1">
        <property type="nucleotide sequence ID" value="XM_037301085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE56"/>
    <dbReference type="GeneID" id="59338622"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140570">
        <property type="term" value="P:extraction of mislocalized protein from mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000538">
        <property type="entry name" value="ATPase family AAA domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF3">
        <property type="entry name" value="FI08533P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="134"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="367"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="389" mass="42912" checksum="3E4CA178DA374E6B" modified="2022-01-19" version="1">MPERTLGVVQDLVLISAASFSIYYLVNHFLSRLDTEGKEQTSRKLAAAAILRRLDPQEKDGESNEKGRRSRKEDLVLTQYEQMIAMDVVAPEDIPVTFEDIGGLGDIIEELKESVIYPLTMPQLYAHTSSLLSAPSGVLLYGPPGCGKTMMAKALAHESGATFINLHISTLTEKWYGDSNKLVSAVFSLARKLEPSIVFIDEIDAVLGNRRSGEHEASGMVKAEFMTHWDGLTSANTSGEPQRILILGATNRMQDIDDAILRRMPKKFAVSLPSAPQRLRILGLILKDTRIDKDHFDLEYLVRVMAGMSGSDIKEACRDAAMVPVKEFIRQKRASGAPMDAVDPREVRGLRTADFFGSAGGIQVLQRPSKQKVLTDSETSEANTDEETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEG2</accession>
    <name>A0A8H6FEG2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3 10">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_010048</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="3 10"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153721.1">
        <property type="nucleotide sequence ID" value="XM_037300904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEG2"/>
    <dbReference type="GeneID" id="59338440"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000001">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000004">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="762"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="801"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6224854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="822" mass="90487" checksum="E2552EC0CF28C3D2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEL2</accession>
    <name>A0A8H6FEL2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Inositolphosphotransferase Aur1/Ipt1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010199</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153871.1">
        <property type="nucleotide sequence ID" value="XM_037301054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEL2"/>
    <dbReference type="GeneID" id="59338591"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF10">
        <property type="entry name" value="INOSITOLPHOSPHOTRANSFERASE AUR1_IPT1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="358"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="6">
        <location>
            <begin position="184"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="409" mass="46878" checksum="6A3EB93DCDAB3CAB" modified="2022-01-19" version="1">MTTNLTILEVPPEWKSQPIQTPPANNASKIVLSILGGAMFLTRRRNFQILGKRKEGYHDSYLDSDSSNSTEDLLWYDSDHDDDSQDPLSASKHLSKRRTCCGLVISTPNSSRFSEHIHSRILQKFPFLVEMFYWIITYLFYRLTKFFSQLIFTKSIIEVAQAHGLTILEFEQFSWASFLFPIKEHNVQHWFMQGHQDALTVLNRAYALIHIPGTVGFIAWYYYIAPSHSTFATIRRTLTLTNLFAFLTFTLYPCMPPRLLPPEYGFLDSVRHDKATSIWMSGNFVNSLAAMPSMHFGYAFVIGCTMIYHAGIFRRSLEKGETKKSCFWKSIYCLIGLGYPSMILVTIVATANHYWMDALMATIVACAAYLCNGVFLTMLPLEDLLLWVLRLEKPIPSTGDTFRQRGGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGJ0</accession>
    <name>A0A8H6FGJ0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155337.1">
        <property type="nucleotide sequence ID" value="XM_037299336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGJ0"/>
    <dbReference type="GeneID" id="59336866"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="233" mass="25898" checksum="1C0CE2A88C565BC4" modified="2022-01-19" version="1">MSTTTTDQKQQRMSELEAMTQRIILSMTTTVTTTVATHMAAMEERIVTRIAEARSRPSSPTIPLSHSTTQSTTQPSTKPVTQPVTQPSTKPVTQPVTQPAHQSTQSATLPEHSVKQEEEQCGTENTAESTAEKASGNKATIIKNTAQLSMIPRQVATQQAENDTAIWPIIPTVLMWYITAMTTQEKDLLRRSQPNHYGYSNWTSADKDHIGRCIFGRIETLSLRQEISRDAHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGZ7</accession>
    <name>A0A8H6FGZ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008923</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155787.1">
        <property type="nucleotide sequence ID" value="XM_037299786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGZ7"/>
    <dbReference type="GeneID" id="59337318"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="8"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6227479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="205" mass="23480" checksum="F18C50D3F7F479C0" modified="2022-01-19" version="1">MDPSQKACARCHNLQADIPLKRCAKCQNQWYCSRDCQKADWKAHKAFCASSQQSNAQREPHATTNFDAMPKVFGNFFKGICPDNYLHQFPKKDAFRQLIDCYRMRVEDDYNFAGDTRGLYNGDDPLADFQEFLDMAETRGGILPSWWSDGKRKECEESAVGTSNWSDLNAAVEKQDVIEHYGDPTMPMKLRLLAEKIYGKKIEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHH6</accession>
    <name>A0A8H6FHH6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">COP9 signalosome complex subunit 3 N-terminal helical repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156404.1">
        <property type="nucleotide sequence ID" value="XM_037299068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHH6"/>
    <dbReference type="GeneID" id="59336597"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055089">
        <property type="entry name" value="COP9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF1">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22788">
        <property type="entry name" value="COP9_hel_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COP9 signalosome complex subunit 3 N-terminal helical repeats" evidence="2">
        <location>
            <begin position="53"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="512" mass="57229" checksum="3EF9A3BB04303FAD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI74</accession>
    <name>A0A8H6FI74_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007124</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156652.1">
        <property type="nucleotide sequence ID" value="XM_037298017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI74"/>
    <dbReference type="GeneID" id="59335524"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042448">
        <property type="entry name" value="CCNB1IP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14305">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CCNB1IP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14305:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CCNB1IP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="12"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="213"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="357"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="130"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="401" mass="44281" checksum="3ABC880EC1454C93" modified="2022-01-19" version="1">MDFTLRCNALNCRVQLVERAVVTTCSHVFCNPCSDELGLTAPAGANRVCPACRTLLSQPDDAVSTSLHPTEDYKTSVLSGLSPSTIMECAGRGLAFWSYQSTQEIVYQEYTVKAMTEKHNTLNVQIDKILNDANSELNILNQKLNNMQIDQDRLKLENKNLVTAFREKSRKHQQTQELYDRLKRKEMTAATQSAAFESVDEVLGNVPGRQGFVSSQRTSVAPRSHAQQDFQSPQRNRNGIEQLHARRKSGSNEIQGNGAMMPPPLHRPGGTGSNTFGFGEPKPWYATHAADGRLANPMPTPSNHRTQLGPTAQSASRLGTLGYRHDANMFNKDLQNHTPGQRQSLASLNMNSVNRNGLSGYGMSAGSRPSEMTHSSHGQAGAPFGHEFQAQQFQRDEGVYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIB8</accession>
    <name>A0A8H6FIB8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_007203</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156731.1">
        <property type="nucleotide sequence ID" value="XM_037298096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIB8"/>
    <dbReference type="GeneID" id="59335603"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20339">
        <property type="entry name" value="BRcat_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16630">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047545">
        <property type="entry name" value="BRcat_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047544">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF47">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="277"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="488"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="548"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="668"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="742"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="802"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="227"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="496"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="553"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="677"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="755"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="811"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6229089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="862" mass="98055" checksum="F5F8BF8F294E52D7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIL1</accession>
    <name>A0A8H6FIL1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156809.1">
        <property type="nucleotide sequence ID" value="XM_037298174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIL1"/>
    <dbReference type="GeneID" id="59335682"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="477" mass="52520" checksum="DF9F745CCA5D78B1" modified="2022-01-19" version="1">MPVNIASKLTSEAGSSNNRQAAAGGGQEPLGIGENRGTVDWYSFIGSDGVGQDQPYLTWPNGKKEQLPILPAIPMTSRYETFLKIRLAPRVVKLTTLLHIFSMYVCHTWYESFGRQLVREEVVGIMRMVFYGTRSNARFLSLERFDDPADELTIALRSMTDTVKDTVKEEWCRHVDGWLQQLRAVDPAAKVRAEDLVGFIGRPPLVLDSTLRAYQVNANSIEGYQACAHYGVVNWGTNRDNEKLREQDTRKVFSHYYNWRAVRAIRLSVIFADPNLQDTQNVALVANDPKYKPFFKALSEQWISKYEVEVLGLKARPNTKIASQSGLVPAMTSSLGALSLTATASSSSQRTAPGPSQLIDIEGSYGFELMTARALLSASPSAPPSESSSKASSGKGKGKETIPETPDYIGQFTDDDDYARSESAGSPPPPGETTSDDPANKGKGKARRRESSSNSPPKTEKGKGKGKEKVAKRQRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ34</accession>
    <name>A0A8H6FJ34_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007562</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157088.1">
        <property type="nucleotide sequence ID" value="XM_037298453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ34"/>
    <dbReference type="GeneID" id="59335961"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF64">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="2">
        <location>
            <begin position="67"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="664" mass="74744" checksum="DD973B1891729A83" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKI0</accession>
    <name>A0A8H6FKI0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUS-like FMN-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_004511</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs. Catalyzes the synthesis of dihydrouridine in some mRNAs, thereby affecting their translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157429.1">
        <property type="nucleotide sequence ID" value="XM_037295428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKI0"/>
    <dbReference type="GeneID" id="59332919"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052582">
        <property type="entry name" value="tRNA-DUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="11">
        <location>
            <begin position="23"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="362"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6230172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="412" mass="45007" checksum="62277A62A9E20B33" modified="2022-01-19" version="1">MASTSKRVPIPKNGVDYRGKVVLAPMVRSGELPSRLLALKYGADLVWGPETVDRSMIGTTKRFNPTTSTIDFTRLPSNSSRLQPSVQKESLIYRLHPTREHGKLIFQIGTASPETAVEAAKLVAEDVAGIDVNAGCPKPFSTTGGMGAALLQTPDLLASILAALVKEVGEVYEIGISVKIRLLETVEKTEALVRKLCKTGITGLTVHCRTTPMRPRERAIREQLRMIAEICREEGVACLMNGDVTDKDEALQLAQDYGVDGGMIATAAEANSSCFRTKEEGGLLPWREVVTEYMKTALEVENKWGNTKFLLSQLMPGKAELFRKAAACMSYRQVCEVLELRDMVDLAKDVDERLGINEQKETKAMKKAKHQNKSAQAAVGNMGKQRSIDKESRSPLSERGVGAMPAMPAMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKV5</accession>
    <name>A0A8H6FKV5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157615.1">
        <property type="nucleotide sequence ID" value="XM_037295614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKV5"/>
    <dbReference type="GeneID" id="59333106"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="81" mass="9121" checksum="391CEAE6DE1D8A89" modified="2022-01-19" version="1">MVFAGEEHIDLDETRALWEPHSKGTPYDLSKAATEKLVPEANCGDLRTLVVSRRVRTHTYNIDKARQALGYNPVPELENGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY64</accession>
    <name>A0A8H6KY64_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007117</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156645.1">
        <property type="nucleotide sequence ID" value="XM_037298010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY64"/>
    <dbReference type="GeneID" id="59335517"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024882">
        <property type="entry name" value="NUP58/p45/49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13437">
        <property type="entry name" value="NUCLEOPORIN P58/P45 NUCLEOPORIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13437:SF2">
        <property type="entry name" value="NUCLEOPORIN P58_P45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21121">
        <property type="entry name" value="Nup49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="173"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6229003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="498" mass="53479" checksum="4D56EE2B2EE1ADFB" modified="2022-01-19" version="1">MFGRSQSGPGGLSINTSSANALPDQSSLPPPSGGKPATSLFGSFNTSQPQQNASAAQAQTSSLFGNTTSQPAQTSSLFGDTTSQPAQTSSLFGNNSQPAQTSSLFGNTTSQPAQTSSLFGNTTSQPQQGSSLFGGNQNTQSGGGFFGSQQANQQTTGGLFGSTNAQQPQPQQTNLFGSVGQNQAQQPQNSLFGGTSDQNKTSSLFGNMPGPAQNQQSTMQPPPARKLFPSQIGQYTQQQQTVPGVRILDVNQLRSTTRFNDLHEDLQKVIENVDNFISGQIRCQEECAQHTEDINQKCHQIPPDVEYCTKALDTTQQALENNAESIAFAKGLVKVDVADAKLSFQTIQNLKMPQQFQQSGMWNTTGISQSGGSSFPEEDLEVGASRTLVEYFSKQADVMSKTLDAYKRSIGQVEAYLKDVESNAMQQMQQMMFDRGQDGVEKSAEEQVRQLAAVLRDFEHGITGVASRVGVTRDKIQEVVLGPDDMVGELSTKRYGMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY82</accession>
    <name>A0A8H6KY82_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_007149</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156677.1">
        <property type="nucleotide sequence ID" value="XM_037298042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY82"/>
    <dbReference type="GeneID" id="59335549"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000595">
        <property type="entry name" value="Protein kinase, putative (AFU_orthologue AFUA_5G11840)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF137">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="433"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="101"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="652"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="679"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="693"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6229035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="799" mass="86146" checksum="45846029A89B5681" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZJ0</accession>
    <name>A0A8H6KZJ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Condensation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004304</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the trichothecene O-acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157223.1">
        <property type="nucleotide sequence ID" value="XM_037295222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZJ0"/>
    <dbReference type="GeneID" id="59332712"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009992">
        <property type="entry name" value="Tri3/Sat12/Sat16/Mac1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42034">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42034:SF1">
        <property type="entry name" value="CONDENSATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07428">
        <property type="entry name" value="Tri3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="56041" checksum="A19751F656E8DCAA" modified="2022-01-19" version="1">MSLLPLIEPSLYNWRPSTKTPNLYLRRALGAETRWARQAPGPRQMFLSGRFTLTGPCSTLSLEAFGNAAEHAWLRVRCEFPEVVLGPSSEQGEDGSILLELKIPGSDGEAREWMRRSLFFGTCEEGNSAEEEMRQAVITHPVCVRLTARVDQEGKVAGAEFAFRVDHMTADGVGAYILAGCYFEFLAHAVSGREATLDWEALKGKLPMPWVGMMNSEQRTEGKEFEEGVKNLTNLVMEASKSEWGMEVVSKDGYMPKAIHKRFSVEESDAILLGVKNKLGKACSVTHLGHAAMVMTMLKFKPVSERPTHGDHLVSPLFINGRRYLDQGVPGSHSHISLCRAFSAIEFRDVESYILSDNASREEVQGKLRLACAEAFRSYQAVRDQKSVLTESLSVAEHMARAKTMKAFKGKWTADAFFLSDGLIETHISNSYCDQTRTHKVLEVDDVQFTASPDGPPLVIRMSTFRQAINLSAEWNGAQHVDEDIRRFLENVAELMLSLIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZN9</accession>
    <name>A0A8H6KZN9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004455</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157373.1">
        <property type="nucleotide sequence ID" value="XM_037295372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZN9"/>
    <dbReference type="GeneID" id="59332863"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="3">
        <location>
            <begin position="5"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="277" mass="28848" checksum="A076ACB398CB4284" modified="2022-01-19" version="1">MAPRIAIIYYSMYGHIGKIAEAEKSGIEAAGGKADVYQVKETLPEEVLSKMHAPGQSSSIPFITPDELEKYDAFLLGIPTRYGNFPTQWKAFWDQTGKQWQTGGYYGKYVGVFISTASQGGGQESTALAAMSTFTHHGMIYVPLGYATAFATLANLEEVRGGSPWGAGTFAGADGSRMPTEQELSLAKTHDWSGGVVANTAAAPSSNQQEPVAAVGTTATSGTTGAGNGASDGTKATTVGTNNPDHVNKSEGAPAAEPVAEKKESGPCGLPFKCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L011</accession>
    <name>A0A8H6L011_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004759</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157674.1">
        <property type="nucleotide sequence ID" value="XM_037295673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L011"/>
    <dbReference type="GeneID" id="59333165"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="210"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="321"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="419"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="490"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6230417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="582" mass="61976" checksum="A547042F48549A0D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L014</accession>
    <name>A0A8H6L014_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157748.1">
        <property type="nucleotide sequence ID" value="XM_037295747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L014"/>
    <dbReference type="GeneID" id="59333241"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="260" mass="27910" checksum="04ED1D7EA22735B5" modified="2022-01-19" version="1">MSPKYVLHTPPPANPHESQPTSTFMPPIFIEMGDEGGEFLEAKIKDETGETVRADVDWTPLSGTRSIDTSVVGGFMFQSDAGSECSIQVVEDGKLPVQILIDRDQDFGQFLVAIQRHEEWKTSGDFHLRFLRRVPILDKRCTAYIKDGPFAGPVPSLSDSGTSITEAPGSSTADAITVEDKEEEQKAADRATGTRKSFPGPTSSDLSFKDAYIVPLAGPTGTSEKTEESTDEEDHLGIGAAALKGTGGGIIPPGMFPPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5U9</accession>
    <name>A0A8H6C5U9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147083.1">
        <property type="nucleotide sequence ID" value="XM_037297647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5U9"/>
    <dbReference type="GeneID" id="59335151"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.280.10">
        <property type="entry name" value="SRA-YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036987">
        <property type="entry name" value="SRA-YDG_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="510" mass="56931" checksum="8B2A99FB9F582215" modified="2022-01-19" version="1">MRLSSRKGASLREELRLHSYYIRDTLVPRLAKEPKLNQSDFLCLQSIFKTLDAVPMTLDLLRYSRIEKALMLMAATRNVCWPMDVLVHAGDLITKWENELGPLKYLRADLYGPGGRMEGVRKMSWKDGRVPDDLGLLREHRSHLEHTPSVTSALRSASTSSLTMIPMKPLPDILNSWWLKPAAAYRDGIVDETSHGITANENGAYAIVLTGSCEIDLGRDGEIRYKAAVSDPGVFRLTKTMLRADPEEKVVRVLRSWKLRSKIAPKAGLRYDGLHVSRPSKGSPTNTRDCHSYRVLSFSVHLSQPNTWQTAFTLSRLPTQLSLLHALHIPTSDQLDDWKDYQRLKLVDRGEDFEMLRDMLAQRGGAGEEVGDGGGLNDERHGSVDSGYFSRRSSKAISIGGGGRRDSILIEEMGETVKGGDNKEDDEGAEEADDEGVGLEDLEEVEEEEEEEENDRKGKVLNSNGDEDGAKNERTAHPNATRTPTPEHLSHAFPQPRIGTRFVSPRKPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6B8</accession>
    <name>A0A8H6C6B8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147280.1">
        <property type="nucleotide sequence ID" value="XM_037297155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6B8"/>
    <dbReference type="GeneID" id="59334658"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="152" mass="17101" checksum="74A5A84D3E356C4B" modified="2022-01-19" version="1">MIQPCLIRVLNHGPPYLDVSSSEEDDSIISHADVDGHEFDYRKILPALVPEGWANDPNDLEPVNRALATDDYSLPPFVLPILASREDGQAGRYLFSIFGRFYLWTPASSEFLSQDTKKLKDIKELVDGHEYGLHFLFRSLSTMGDMAVQSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6F8</accession>
    <name>A0A8H6C6F8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase C14 caspase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C14B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146914.1">
        <property type="nucleotide sequence ID" value="XM_037297713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6F8"/>
    <dbReference type="GeneID" id="59335217"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12660">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050452">
        <property type="entry name" value="Metacaspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104:SF30">
        <property type="entry name" value="METACASPASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104">
        <property type="entry name" value="METACASPASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C14 caspase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="327" mass="36215" checksum="792843863594F512" modified="2022-01-19" version="1">MSQYNAQGYQNSYGPRTTHRKSLLIGINYEGQQSALRGCRQDVRNMSQFLISRGYPTDERSMVILTDDRQGPFYASGHNILAAMDWLVSEPNTQCFLHYSGHGGQVKDPDGDRESGYDDTIVPVDYQTKGQLDSDTLHRHLVSKLHPTSSLFVVFDCCHSGSAIELPFVYRTDSDGNVTLVDSVKEGISLLAHAQGLLQGGFSASKINDAKALFAGAQSFFSGLKHRGEHQEEGLGEEHFQEDWAHEGRSVFMFSGCRDDQTSADANIAGSHVGAMSWALLETLYSRPGQSYVEVLQNTRQLLLGKYKQIPQLSVGSQVDLNQPLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6R6</accession>
    <name>A0A8H6C6R6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147131.1">
        <property type="nucleotide sequence ID" value="XM_037297421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6R6"/>
    <dbReference type="GeneID" id="59334924"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="664" mass="73620" checksum="AC943A9065F587DE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6V9</accession>
    <name>A0A8H6C6V9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_006419</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147442.1">
        <property type="nucleotide sequence ID" value="XM_037297317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6V9"/>
    <dbReference type="GeneID" id="59334820"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="28"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="222"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="311" mass="34505" checksum="3C64EC4316B05FB1" modified="2022-01-19" version="1">METNNSGRDQVVRVTVACGILATLAVCLRFVARWRSKATFGADDWWMAASLIPSYCMLAVGSIMITSGKGGHHADTLTESQMATFLKTLDASLITYTLTITMVKISILLLYRRIFTTPSFKRATTVVGIACISWLIAALFCLAFQCHTDAERYNADSIFSSQCIDLKAYWAATTGSNMALDLVILCMPLYMVWNLKLATHQKIWLSGTFALGTFWTSRRTASSSSAKNNGRRSDLGIDPESDQKIEKKPMRWPSERRKSDMELSGFEQLANARIPGGISFLEKAESERTEHNLQDDGGSTMARAKSEESFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7A6</accession>
    <name>A0A8H6C7A6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005641</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147730.1">
        <property type="nucleotide sequence ID" value="XM_037296549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7A6"/>
    <dbReference type="GeneID" id="59334046"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF1">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="29259" checksum="E9F39E2B008EDE63" modified="2022-01-19" version="1">MDKKMSGISKPDTDIPKLSGESKHTEALAIAPETVQLVWNYSKMSVTEIPDHWMDVSPVYKKMKARMLKGEKLLELGCGFGQNMRKLCVKGVAAEDLFGFYTDRILIEAGYDLFVDRSTQMTFFTGDIVAGTFEFERFHDKFNIVFASMFFHLFDWTEQVAIAKQVVNFLRKQPGSMIFGQQIGHIKPGLYQVGRESAGYAHNTATLQQMWDFVGSETKSTWHVQGIMEVNENIAALTQDDNTRCLRFWIRRLWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7L7</accession>
    <name>A0A8H6C7L7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147596.1">
        <property type="nucleotide sequence ID" value="XM_037297022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7L7"/>
    <dbReference type="GeneID" id="59334524"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="326" mass="36286" checksum="3D0048BA90CCA362" modified="2022-01-19" version="1">MAKIPLKIRMDLRDHWEKEDCPARKSIAALKDLLGLPVTIQLEPLILWSELQKFYSDQSTFVPCITTVIKAWLDCLTKRLADDANAAWTEQLLEHVNQSGRNLKARVETRPGTQVKTELAKTGATFTIGIPESLPPYKNVSACFMTEFEKLFTKSSKQTGSAQAQGAEDEDWADVTMPSKTRGQTTDILSTTMPSLDELPRPEMLFAAQTPYHLIVKVVHNGMVVQGSHQGSLELLTGYLQKYTRDIENISVKLPILKSELQASHFGYGALFDSLTITPGDSRAGWASINPILILSFIESVLGYSPVASGSTESQWYFKRDVGFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7S6</accession>
    <name>A0A8H6C7S6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ZZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005778</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147864.1">
        <property type="nucleotide sequence ID" value="XM_037296683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7S6"/>
    <dbReference type="GeneID" id="59334183"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14947">
        <property type="entry name" value="NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02249">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02340">
        <property type="entry name" value="ZZ_NBR1_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02341">
        <property type="entry name" value="ZZ_ZZZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032350">
        <property type="entry name" value="Nbr1_FW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041981">
        <property type="entry name" value="ZZZ3_ZZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF0">
        <property type="entry name" value="PROTEIN ILRUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
        <property type="entry name" value="N_BRCA1_IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="6">
        <location>
            <begin position="436"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="561"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="775"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="581"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="809"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="828"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="869" mass="96031" checksum="5B70B3E907DB987D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C801</accession>
    <name>A0A8H6C801_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="2 6">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the DTD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="2 6"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147969.1">
        <property type="nucleotide sequence ID" value="XM_037296788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C801"/>
    <dbReference type="GeneID" id="59334288"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051500">
        <property type="term" value="F:D-tyrosyl-tRNA(Tyr) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.80.10:FF:000001">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00256">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472:SF5">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="D-TYROSYL-TRNA TYR DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="DTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="148"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="193" mass="20920" checksum="432071BC335587F5" modified="2022-01-19" version="1">MKAVLQRVASASVTVDSNLVSSIGKGVLVFAAVAKDDTRKEVESMAAKVLKLKMWPDESGGTWKQNVQDIKGEVLCVSQFTLLASTKKGNKPDFHGAAGGEEARELYDQFFAKVQELYEPAKVKNGVFQAMMEVDIQNSGPVTLEIDTNPHKGQDGNKKPGNEQGEHQQSRASADEGKGKVAESFQLPVELLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C828</accession>
    <name>A0A8H6C828_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148122.1">
        <property type="nucleotide sequence ID" value="XM_037296941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C828"/>
    <dbReference type="GeneID" id="59334443"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="246" mass="27049" checksum="59231A9F20DDF725" modified="2022-01-19" version="1">MRYSHVCTLASALASITILTATTVLASHLQLEKEPLLGPPALGPTSGYDDPRDPSSIKSGSPRSRDLDQAHTQDLMSLRQPTSTASKRRPNIYHSHLVWSTSRSKAGYSLSVSERSLLTDLTMIGFRLVWDYMDIAYTSYIAFHQTTELWANITANARGKWKAEKEVMTLDIIYGSLKLSIAGLVDAISWELLADFAAEMLVLSRNIVVVAFSVIIFANWAAMVITLVIAVQLLGTIKPQQLITGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8K0</accession>
    <name>A0A8H6C8K0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005527</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148418.1">
        <property type="nucleotide sequence ID" value="XM_037296436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8K0"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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            <end position="205"/>
        </location>
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        <location>
            <begin position="629"/>
            <end position="730"/>
        </location>
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            <end position="28"/>
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            <end position="41"/>
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        </source>
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    </evidence>
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            <dbReference type="EMBL" id="KAF6218983.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9D1</accession>
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            <fullName evidence="4">DUF6594 domain-containing protein</fullName>
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        <name evidence="5" type="ORF">HO133_004938</name>
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        <name type="scientific">Letharia lupina</name>
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            <taxon>Lecanorales</taxon>
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        </lineage>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6219113.1"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="552" mass="61248" checksum="0DBDFD4013E86ABB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9M2</accession>
    <name>A0A8H6C9M2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VanZ-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">HO133_005088</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
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            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037296000.1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="VanZ-like"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VanZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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        <location>
            <begin position="25"/>
            <end position="175"/>
        </location>
    </feature>
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        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
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        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
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        <location>
            <begin position="32"/>
            <end position="109"/>
        </location>
    </feature>
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        <location>
            <begin position="121"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="175" mass="19046" checksum="E4AFAC2FEBD33845" modified="2022-01-19" version="1">MRVRINFACAFVLVLLGAAYLGLGSTQIPQVNDKVLHFLTFFLLTTAFYWILDTTRRRVLNFTLLVVTFGLGLGSEALQGLLPNGRQFDPLDIAANVVGSLLALGLCTLYHKRMLDRRRRAKGYSTVPQDGEGQDLELGGQETGTVEEDARESSPNGEGRLTPSSGAEEGIEGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA75</accession>
    <name>A0A8H6CA75_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">HO133_003964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148931.1">
        <property type="nucleotide sequence ID" value="XM_037294884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA75"/>
    <dbReference type="GeneID" id="59332374"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="516" mass="56850" checksum="60B9F756C549D06D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAS1</accession>
    <name>A0A8H6CAS1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EDC4-like protein pdc1 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003718</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat EDC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149328.1">
        <property type="nucleotide sequence ID" value="XM_037294640.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332129"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000817">
        <property type="entry name" value="WGS project CABT00000000 data, contig 2.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.100">
        <property type="entry name" value="conserved c-terminal region of ge- 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055393">
        <property type="entry name" value="Beta-prop_EDC4L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045152">
        <property type="entry name" value="EDC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044938">
        <property type="entry name" value="EDC4_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598:SF5">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24106">
        <property type="entry name" value="Beta-prop_EDC4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EDC4-like protein pdc1 beta-propeller" evidence="7">
        <location>
            <begin position="533"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="233"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="302"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="986"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1364"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="242"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="986"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1015"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1050"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1072"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1088"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1107"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1377"/>
            <end position="1386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1559" mass="168924" checksum="CDF0878B9146F368" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBA3</accession>
    <name>A0A8H6CBA3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002827</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149830.1">
        <property type="nucleotide sequence ID" value="XM_037293752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBA3"/>
    <dbReference type="GeneID" id="59331239"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="48175" checksum="966A9C1C4EF95B21" modified="2022-01-19" version="1">MSGHSALELLLPEVQQQILLRLDFVDLQALIHASPRMYQVFRLNKRTILSTVTRQRFDPATTRAAQAIERLYSIEQPPFSRDTLLRMFSTLDELDDSLDIVLPLPVSTTLGRLDGTIRFFADDYTRNTFPILGQLGQSGFVTIETEYEQNPHGPRSDISRSESNRLHRAFSQFETYRQFFSQCSSDFNHGPRQCFGEPSLTVYEQAKMFCQDMPAYQAAEIACVRDYLYRRLRGVHDQVEDEVVQKLQAGCPDPRDKYEGLDWDWDNGGRHQYLADDEHIFGYSGKYMQNSHIEYLLSLGLPYIRRILESTGDKRQDLLLHTESRCYAHHQTNFITAALGLDPLDSNDDQYGWYERDIDSCLDENGKLDLPPGWLWAHTGDYYHGLVDVTAKGLRDWGYVFWDLERLQKTGILDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBX6</accession>
    <name>A0A8H6CBX6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150037.1">
        <property type="nucleotide sequence ID" value="XM_037293959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBX6"/>
    <dbReference type="GeneID" id="59331447"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007541">
        <property type="entry name" value="Uncharacterised_BSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33321">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33321:SF12">
        <property type="entry name" value="PLANT BASIC SECRETORY PROTEIN (BSP) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04450">
        <property type="entry name" value="BSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="330" mass="36806" checksum="6EE9EC49C41A23E5" modified="2022-01-19" version="1">MTRTVRTTPKLLPTPLAMSAAPNEPTPSPLPIRPKEEAHLFKEAGRKKDGREEEVTSRPHPKPTFPTPKLRLSTLDLDHPGSNVFFSNTNPSKALSEAVDAVLSILYQPTKTNDHIPACRSVTLCLHAMGGVAYTTGSDLDGDHKEIHFSLDYINGIPSARQKDEVQGVLVHEMVHCWQWNAVGTAPGGLIEGIADFVRLKAGLSPPHWKKEGGGQWDAGYQHTGYFLEWIESTCGRGSVRKINHALKDKKYAEDDFWEQLFGKNVSLLWKEYEGSLDEEQKMTKKVESSAKSHEKDRKDNAEMKEKGRKDGRSEGDESVEKDKPNDNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC72</accession>
    <name>A0A8H6CC72_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150187.1">
        <property type="nucleotide sequence ID" value="XM_037294109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC72"/>
    <dbReference type="GeneID" id="59331597"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="278" mass="31448" checksum="0A8392150A286111" modified="2022-01-19" version="1">MALSSKIELASLGEAGNETPVSQSQETLTAAPFGTLTLVPPEIREQIYLDLIAAGSVRFLESSKAFHNEALDILHQKGVCRLEFNIFKPRDIFPSQQFSKPILNLEIRLDLTDFVLRVPQPSNTCINKFNEWLTQSMDCVDTSRGLLPPTHPTPRKCCKILLDCNPGFSGWIPKFTFDFMKSLTGFKALVLAVANDYPGERKRRMVERMYDPRPNPQRDMESAMHSKENEDTLHVYETAMKALEPTLGPAEWVSDEKGAHLEFHPRAHNFSTDDGRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC74</accession>
    <name>A0A8H6CC74_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_002555</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="12"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150310.1">
        <property type="nucleotide sequence ID" value="XM_037293481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC74"/>
    <dbReference type="GeneID" id="59330968"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000004">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000040">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="343"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6220875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="400" mass="45102" checksum="FD2F33B910644C5F" modified="2022-01-19" version="1">MAEQLSREEKLKLIYQNLQEVLKPEIIEQVVLKEDRPLKIYWGTATTGRPHCAYFVPMVKLAHFLRAGCKVKILLADIHAFLDALKAPIELVKERAKYYRFVITSLLKAINVPIDQLEFVMGSDYQTSSNYTMDLFKLSSVVTEHDAKKAGAEVVKQMDNAPLSGLIYPLMQALDEQYLDVDAQFGGVDQRKIFTLAAEQLPRINYKERAHLMNSMVPGLAGGKMSASDPESKIDILDTPDAVKKKLKKAYAVPKEVTDNGLISFVEYVLLPVSALKTEGKGKFVVERREGEPLVYDSIETLKDDYTADTLTPQLLKSGVTDALNALLEPIQAEFKASKEWQEVEQKAYPPPEVKKKPKKEKKLGDRFPGVIRDQVKPDDKVEEEQRKHEENLVTGLSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCA2</accession>
    <name>A0A8H6CCA2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003047</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150049.1">
        <property type="nucleotide sequence ID" value="XM_037293971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCA2"/>
    <dbReference type="GeneID" id="59331459"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF158">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 12-LIKE CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="51503" checksum="6A2A3B4F7A3DB2A8" modified="2022-01-19" version="1">MSGNRLRTARVLSVIAATLISLACGTNYAYSAWAPAFAEKLKLSSTQSNLIGTFGNLGMYSAGIPIGLLVDTRGPRPGALLGAVSLGVGYFSLYRAYDGGPGSISLPWLCFFAFLTGVGGCASFAGAVKTSALNWPDHRGAATAFPLSAFGLSAFVFASVSSLAFPDNTLDLLLLLAIATFTIPFIGSFLLRIVPVPQSYDPLPGSLERSGSDASTLLQRSKSEDSRHIVRHLSHEPDIRGLAMLSHTKFYLLWVLMGLLTGLGIMTINNIGSDARALWHHYDDSASSEFVQERQLMHVEILSFMSFCGRLTSGIGSDIIVRKLGMSRFWCLFISSTIFLFAQVCGTQIDNPHLLGFLSGLTGLAYGFLFGVYPALVAETFGVNGFSQNWGSMTLAPIVFGNIFNLVYGHIYDRHSIILPGGQRDCKDGLYCYRNAYFVTLGASLVGVGISLWSIHHDWIANLKKRKERERAREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCK7</accession>
    <name>A0A8H6CCK7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002496</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150251.1">
        <property type="nucleotide sequence ID" value="XM_037293422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCK7"/>
    <dbReference type="GeneID" id="59330909"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056693">
        <property type="entry name" value="DUF7791"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF5">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25053">
        <property type="entry name" value="DUF7791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="3">
        <location>
            <begin position="361"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7791" evidence="4">
        <location>
            <begin position="579"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="662"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="662"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="675"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1153" mass="131034" checksum="E092DDF1CB1B2E41" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCU2</accession>
    <name>A0A8H6CCU2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ecp2 effector protein-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150597.1">
        <property type="nucleotide sequence ID" value="XM_037292944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCU2"/>
    <dbReference type="GeneID" id="59330430"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029226">
        <property type="entry name" value="Ecp2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14856">
        <property type="entry name" value="Hce2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034730385" description="Ecp2 effector protein-like domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Ecp2 effector protein-like" evidence="2">
        <location>
            <begin position="55"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="185" mass="19748" checksum="BCAD8807E51EA4A9" modified="2022-01-19" version="1">MALLGENIRGNVLHILTLAAAFSLVLTSPATAPTEPSPGVIANTTILPGPGDKYCDTDGSDFENGSSSASPLIEDCGNLSSIMTSAPNDDNPYGWWEVQHCTPNCKWWLAKYQTCEFGVSADGPFGAYKLGITRVDIAAILDLSIEKFSWYKQVGASGDMQCNIMNGADEPTGEQHGMSWSIAHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCW9</accession>
    <name>A0A8H6CCW9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Smr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150700.1">
        <property type="nucleotide sequence ID" value="XM_037293047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCW9"/>
    <dbReference type="GeneID" id="59330534"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053020">
        <property type="entry name" value="Smr_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417:SF1">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="2">
        <location>
            <begin position="99"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="274" mass="29981" checksum="53292B48A5C1945B" modified="2022-01-19" version="1">MSYPMTQLGGSAFNHQPPSSSTEAEYDRLRDLARQEAGKRSSCLAKSHQAFERHDGGAAHALSEEGKRHGAMMDQYNKQASDYIFRENNAVGRVDGDTIDLHGQFVEEAEQILEQRIRYARSTGQNHLHVIVGKGNHSPGHIQKIKPKVEEICQELGLQYATEENAGRIYVNLQGGPAVMPEHLKAHHGGGYHQGYGGPGYGQQQQQQQGYQGGNTYPQMNYQQGGGGQQYQGGGGGGGGGQQQQDPNAAIEAEVKKYLPQVLRMCQKQCCTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD21</accession>
    <name>A0A8H6CD21_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_002642</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-X family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150396.1">
        <property type="nucleotide sequence ID" value="XM_037293567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD21"/>
    <dbReference type="GeneID" id="59331054"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00141">
        <property type="entry name" value="NT_POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000010">
        <property type="entry name" value="DNA polymerase lambda"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.210.10:FF:000001">
        <property type="entry name" value="DNA polymerase lambda"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.110:FF:000005">
        <property type="entry name" value="DNA polymerase POL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.210.10">
        <property type="entry name" value="DNA polymerase, thumb domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002054">
        <property type="entry name" value="DNA-dir_DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019843">
        <property type="entry name" value="DNA_pol-X_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028207">
        <property type="entry name" value="DNA_pol_B_palm_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018944">
        <property type="entry name" value="DNA_pol_lambd_fingers_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037160">
        <property type="entry name" value="DNA_Pol_thumb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022312">
        <property type="entry name" value="DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002008">
        <property type="entry name" value="DNA_pol_X_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276:SF28">
        <property type="entry name" value="DNA POLYMERASE LAMBDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276">
        <property type="entry name" value="DNA POLYMERASE TYPE-X FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14792">
        <property type="entry name" value="DNA_pol_B_palm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10391">
        <property type="entry name" value="DNA_pol_lambd_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00869">
        <property type="entry name" value="DNAPOLX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00870">
        <property type="entry name" value="DNAPOLXBETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00483">
        <property type="entry name" value="POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81585">
        <property type="entry name" value="PsbU/PolX domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00522">
        <property type="entry name" value="DNA_POLYMERASE_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase X" evidence="18">
        <location>
            <begin position="401"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="16"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="259"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="286"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; Schiff-base intermediate with DNA; for 5'-dRP lyase activity" evidence="16">
        <location>
            <position position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR622312-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6220961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="728" mass="82369" checksum="174FC105F9C2C019" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD56</accession>
    <name>A0A8H6CD56_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CsbD-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150712.1">
        <property type="nucleotide sequence ID" value="XM_037293059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD56"/>
    <dbReference type="GeneID" id="59330546"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036629">
        <property type="entry name" value="YjbJ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40460">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40460:SF1">
        <property type="entry name" value="CSBD-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69047">
        <property type="entry name" value="Hypothetical protein YjbJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="18141" checksum="8E8CF31B1AD14FBC" modified="2022-01-19" version="1">MSDNQTSSLKSYIDSATGTVQSTLGSLTGNTADQAKGDVTKDHAAAEKDASHTIGKIGPYAVSSSGGISSDDPRRTEGSYDQTMGSAKEAVGGLFGAEGLKKEGQQQNAQGKGTEAEGQLSDYGHGLKDRVGGTVGGIAAGITGDSEKQQEQQLKHDDAKTKQRSAEHDIQKQADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD64</accession>
    <name>A0A8H6CD64_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Multifunctional tryptophan biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="16">Anthranilate synthase component 2</fullName>
                <shortName evidence="16">AS</shortName>
                <ecNumber evidence="16">4.1.3.27</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="16">Anthranilate synthase, glutamine amidotransferase component</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">Indole-3-glycerol phosphate synthase</fullName>
                <shortName evidence="16">IGPS</shortName>
                <ecNumber evidence="16">4.1.1.48</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
                <shortName evidence="16">PRAI</shortName>
                <ecNumber evidence="16">5.3.1.24</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_002147</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 16">Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 16">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 16">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 16">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="16"/>
    <dbReference type="EC" id="4.1.1.48" evidence="16"/>
    <dbReference type="EC" id="5.3.1.24" evidence="16"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150727.1">
        <property type="nucleotide sequence ID" value="XM_037293074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD64"/>
    <dbReference type="GeneID" id="59330561"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000136">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000031">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016302">
        <property type="entry name" value="Anthranilate_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001382">
        <property type="entry name" value="TrpG-trpC-trpF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 16" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8 16" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="18">
        <location>
            <begin position="27"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="19">
        <location>
            <begin position="268"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="20">
        <location>
            <begin position="616"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR001382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6221292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="796" mass="86443" checksum="9FAD65B0B092AAA7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDA2</accession>
    <name>A0A8H6CDA2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002284</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150863.1">
        <property type="nucleotide sequence ID" value="XM_037293210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDA2"/>
    <dbReference type="GeneID" id="59330697"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="374" mass="41856" checksum="345DBEE414756101" modified="2022-01-19" version="1">MKTPPIRSNSNPVAASKRTAFTVILAIALFLWMISPFSASPRQLDRVPFSQNSVEDHFSHFEGHGDCGIRSTQLYVPPVAPDDDAPKHTLYCNNRATLLEAMSGGGRHGFEKPYFPAGCHYRWYSTAEICMILERFDAIVFIGDDSLKHIYAAFNMLLRENMAMGSLKQWELNESQRDACRCDNQFTKSECWSHMIMESQSVKEKEDGVGHRNPFFCDRTPHVFLPVTDSPAPEELHASFTSLISGDPDAYKPIPVIHSLSLATSLSWSKAVDSMDEWVTLADASGRNIPILWVGPNTAGHLKPPGQILTQGNNALWHYTLETMKEAKARQLDALGMYNLTLQANSWDGSNYGQRVGLVQAMMVINWLSRVEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDI0</accession>
    <name>A0A8H6CDI0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150983.1">
        <property type="nucleotide sequence ID" value="XM_037293330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDI0"/>
    <dbReference type="GeneID" id="59330817"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="53558" checksum="4503508C6B468008" modified="2022-01-19" version="1">MDNASAQLAIALQLHDLDELEVSGTADKLVIRLQRQQLEIDSGFDAVTFEASRRLALSMAKAVEDDSALLARSTPLPQIDDTTFDRLALLNHAPSTVSNHSKLAPANSANQTLPSKATSQKRARSLTPEGDPSSVSVNPKEAELRCPSHAALDHVGHSHKKVKCQQITNGDHPRIQVEGSGIYSVQDPQPSTAEASGAQVVKETAAPTTAFETSPTTGDCASCSDHLAVDELVKASCKHYYCKDCFGHFIEASLQTHDGFPPKCCKIPIAFVTVADNVSAVVFSRYSSRQAEIKNATALYCGIQGCGVRIEKGRIEGVRATCVTCWRDTCTQCRAEFPQNVNGRNVGHVCKKDKAHEEVLALAKKEGWQTCYQCGHMTSVQLAASPEVFLLIYALASSSWQSSSRDCRSTVTTTTSTTREANLNKIQGAVRSVSILPGYTSFAANIADSRPAEIVTNFFGWSRGENLWGLRFGARNYDDDTDDEPVSEDQSIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDN5</accession>
    <name>A0A8H6CDN5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150987.1">
        <property type="nucleotide sequence ID" value="XM_037293334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDN5"/>
    <dbReference type="GeneID" id="59330821"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="152" mass="15754" checksum="E9D30699929827DA" modified="2022-01-19" version="1">MSANTSKEMPAKVAQTSKASTPRSTSSNAASTKATSGTPGTTLRHQPAAQDPNQALSQRLTGPSGNTLLRGIGAWADPPGPNAGATTDSAIGGDISHQRNARELGPQSQPRSSNDQGLTPMERFRKEGRHDQPSGLLDGGKPQSEHGKGKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDS4</accession>
    <name>A0A8H6CDS4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150756.1">
        <property type="nucleotide sequence ID" value="XM_037293103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDS4"/>
    <dbReference type="GeneID" id="59330590"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="133" mass="14106" checksum="CE0C62CBB94F96F4" modified="2022-01-19" version="1">MQQNFLDGTVCGGGGKCNNGQCKGSTAAKEIGSWIHDNRNLVIGICCGIGGLLLLSILSCIVRRCRRPRQPRRTQPMMPPMNQGWGGPMPQGAWMSGAQQGQGRGGSGHGYGGYKGGPQYPPLGYVQPSVRYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDV2</accession>
    <name>A0A8H6CDV2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001571</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151040.1">
        <property type="nucleotide sequence ID" value="XM_037292501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDV2"/>
    <dbReference type="GeneID" id="59329987"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054293">
        <property type="entry name" value="DUF7029"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055647">
        <property type="entry name" value="DUF7223"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22974">
        <property type="entry name" value="DUF7029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23865">
        <property type="entry name" value="DUF7223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034248153" evidence="2">
        <location>
            <begin position="21"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7029" evidence="3">
        <location>
            <begin position="92"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7223" evidence="4">
        <location>
            <begin position="260"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="597"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="690"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="736"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="769"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="804"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="813" mass="85494" checksum="8BBF46CBA95A04DE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEG1</accession>
    <name>A0A8H6CEG1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151478.1">
        <property type="nucleotide sequence ID" value="XM_037292059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEG1"/>
    <dbReference type="GeneID" id="59329545"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="244" mass="28218" checksum="51D242F58025EDDC" modified="2022-01-19" version="1">MVHFYDLPYEIRQMIYAECLVVGEIHPYSLRDTSLRDRHSAWAKTGCDLPTVALLQVSKTIRTEAEPALYERNMVQLGSAEFSQRFFERCLNTSERKLWLKSVSMWLDDGEMTEADQRAVQDTLFPERAYTKVPWVKKVQDAYKQYIGETVWPRKVLPLLDSCKLNTLVMDFSEALCQADCCTMRSHAVQSFRKGFAMGLPARFVIQGLGTGAKASRKLIQRWTSRRITDLDRKLVLDIFSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CET7</accession>
    <name>A0A8H6CET7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7719 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151627.1">
        <property type="nucleotide sequence ID" value="XM_037292208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CET7"/>
    <dbReference type="GeneID" id="59329694"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056136">
        <property type="entry name" value="DUF7719"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37846:SF1">
        <property type="entry name" value="DEACETYLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37846">
        <property type="entry name" value="YALI0B21296P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24841">
        <property type="entry name" value="DUF7719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7719" evidence="3">
        <location>
            <begin position="149"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="218" mass="23994" checksum="7B5331450530FF87" modified="2022-01-19" version="1">MSNRRERRVQARANPATKNETPLHQPSRKAPTHKTLFDIASERQLLATTSQSSPPSITTTRINPDGTLSTTGSASNSPDATMPYLDVFLYTATLTMLHFTLTVLVHHQYATTPPSLTSILYTSTVISPTPVLILVLVSILHPQSSHLLTQILFACLSVAAGAWLVHASNEDPYMAVMKKAPPLGTLWVWAIVEMRWEWAVGCLGVVAGWGWWKGYSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFR8</accession>
    <name>A0A8H6CFR8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dynamin GTPase</fullName>
            <ecNumber evidence="3">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_001477</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151826.1">
        <property type="nucleotide sequence ID" value="XM_037292407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFR8"/>
    <dbReference type="GeneID" id="59329893"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000741">
        <property type="entry name" value="Putative mitochondrial dynamin GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056495">
        <property type="entry name" value="LIS_MGM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF212">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24550">
        <property type="entry name" value="LIS_MGM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13 17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="20">
        <location>
            <begin position="242"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="19">
        <location>
            <begin position="808"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="904"/>
            <end position="925"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6222391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="925" mass="101781" checksum="46BBF016B5448DAE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGB9</accession>
    <name>A0A8H6CGB9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000914</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152209.1">
        <property type="nucleotide sequence ID" value="XM_037291847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGB9"/>
    <dbReference type="GeneID" id="59329332"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="10"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="326" mass="35873" checksum="7FD8A3C50DD2CA6D" modified="2022-01-19" version="1">MVKVLLTVQELIADDRHDVVTTVRSKAKGDKILEAYSNTLKEKLSYVIVEDVAQDGAFDQAVRTDPPCECVIHTASPFHFKVKDPVKDILDPAVKGTSGILKSIKAYAPTVKRVVVTSSFSAMFGLNAHPKVYDETSWNPVTWEGAVKNPRLTYHGSKTFAEKAAWDFVEKEKPNFDVVTINPPLVFGPVTHYVSSLDGLNTSNERVRDIIQGNFLDKALPHTGIYLWADVRDVALAHIRAIEVPEAGGNRFLVTAGHFSNKIVVDMIRESHPELSSKLPENPTDDIPADIYGYDSSKASRILGITFRSLKDSIGDTVTSLVKLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGG6</accession>
    <name>A0A8H6CGG6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001053</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152348.1">
        <property type="nucleotide sequence ID" value="XM_037291986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGG6"/>
    <dbReference type="GeneID" id="59329471"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF146">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="31"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="331"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="334"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="454" mass="49839" checksum="0B965ED8CCFFF1EB" modified="2022-01-19" version="1">MSHSFCDENQGTIGLAFTWILLSFGILTVSLRIYVRSGVNGKIGWDDYTAVASLVSTPSYLRFAFTSVVARFPNRMKDKWLTLALIQAVGILAGGFFTKMITSGMGRHVSCLPPQSVIAMLEFSAISEALNVFGIGVVKISVCLALLRVVERARRGITLFLRSLLVFVAVTHLALALLFFLHCRPLAALWNPKVQGSCLSTHTTVLAGYVGFAVDVVTDLVCAGIPVLVIHRLQMSFRTKVALCVLMGLGVFTAGCAVAKAITLRGVFADDYTFGFTKPATWAAVEQFVGIIITSIPALRPLLRSFLEQSHNRSGIRRYIFWGSKTGSFGFGKKGSPQQQEQQQQQYQQQKQQQVSRSESSVQESVRTKNTFLPWHRDSRGLPFSSSDSNQATAVYKESSKGGTGQVDPEKVLLGDRFGDRFGDVVGRDYLKGWSLPDIADRRSAMIAIPVFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHG5</accession>
    <name>A0A8H6CHG5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152794.1">
        <property type="nucleotide sequence ID" value="XM_037291359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHG5"/>
    <dbReference type="GeneID" id="59328839"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="492" mass="55118" checksum="DF27D7FF1CE95FFE" modified="2022-01-19" version="1">MTLTRLPTELLEIIITHVLPEGFESVAVTCRKIYALCIPFIQRHNALRSRFHHFTYHENPNDPSPTIATAADLIRRIAVEPVVARYVRYADFKRDSPRSYIIPRGFVADADCRDDVIRLFANCPYLEQAGLDWKEYVDKIEEEFNPDELPRYSQHAAAFLLTLLPNVERLILPQYWKPLDATNKLIHAVVHKTKQVHSLYDRPSLAQITRFEPYVSLVPQDRFDLGLASPFLALPRIRSFRGPSCVIMDDNHKSIIPKDPHYGFGETLESVSLVACCVDEVGIADFLKNTKRLKSLRYSHSTKYNVSPQCWDICKFVAAIERQVGSHLVELSVSIGKPFGSIAPGKASMRGFPCLRQLEFPLEIVMCNITAAACLDSTPNDSLVGGSSDHKLNCVALFVGDLVPASVSVLSLISHGTDHHDKALDVMFQDFAARKEITLPALTDIHLICPNGADDAYKDRGTRLLVETQKAGVVLHLEPWAHSGALTWDGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHI4</accession>
    <name>A0A8H6CHI4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Swi5-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000366</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWI5/SAE3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152740.1">
        <property type="nucleotide sequence ID" value="XM_037291305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHI4"/>
    <dbReference type="GeneID" id="59328785"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032798">
        <property type="term" value="C:Swi5-Sfr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034974">
        <property type="term" value="C:Swi5-Swi2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010772">
        <property type="term" value="P:meiotic DNA recombinase assembly involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000709">
        <property type="term" value="P:meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010760">
        <property type="entry name" value="DNA-repair_Swi5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07061">
        <property type="entry name" value="Swi5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12676" checksum="77F079F08106B5CF" modified="2022-01-19" version="1">MSSSPTSTQEVPKPGPLTASPSSPAQADPLEAKKIAALEAKIAALESQIRETEDKRREVDGQLKTKDPQATVKAHIKLLHDYNEIRDIGQGLMGIIADNRGVPVREVYQDFNVGEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ12</accession>
    <name>A0A8H6CJ12_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153438.1">
        <property type="nucleotide sequence ID" value="XM_037301804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ12"/>
    <dbReference type="GeneID" id="59339345"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038213">
        <property type="entry name" value="IFI6/IFI27-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="114" mass="12232" checksum="9F7640732AA9FF42" modified="2022-01-19" version="1">MTNYVNSLTLSARRNGLVVGGPLPAQALTKLMSHGKRLIGYLKQPRSLAAAFQSVVYGGFVPAGGLFASLTSMGMLGTLAPLFLGVAALVATAVTVMVWGFRVRRWRNMGRQHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK50</accession>
    <name>A0A8H6CK50_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010224</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153896.1">
        <property type="nucleotide sequence ID" value="XM_037301079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK50"/>
    <dbReference type="GeneID" id="59338616"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF1">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="31795" checksum="E2D68846FCF07112" modified="2022-01-19" version="1">MATEIQTTAPTGTGQSKDVADYIHDADLPDLPPAVRTLFEEYSKIPAAEVQPHVLQLRERAWQIFPYPCIGRLRFLDFSLSTMPSYDKILSSLSSKEDPKTLMDVGCCFGQDLRKLLLDGAPGSQLVGVELKPEFIDMGYDLFMDRKTYQGRFVTGDFFEDTTGSAMKSLDGTIDILHIASFLHIFGWDEQVRAAVRLMGFLKNKPGTIILGRQLGSSKPGEYRHPAGVVGVTYSHDEETFQKLWTEVEKKTTTKWKIEANLKKVPTKGSTLELLYFEATRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK71</accession>
    <name>A0A8H6CK71_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153830.1">
        <property type="nucleotide sequence ID" value="XM_037301013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK71"/>
    <dbReference type="GeneID" id="59338550"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="86" mass="9063" checksum="0B1788357EFD78B2" modified="2022-01-19" version="1">MTEDWADKGGAVLATRPDGAIVMQRAEMEIIVHSGPGLRFWGVGSGEGGGGRWRGRGGGEDEDEEEEEENGEGKEVGASGNEAWDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKB6</accession>
    <name>A0A8H6CKB6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010407</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154077.1">
        <property type="nucleotide sequence ID" value="XM_037301260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKB6"/>
    <dbReference type="GeneID" id="59338798"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034765728" evidence="2">
        <location>
            <begin position="22"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="144" mass="15035" checksum="59912F5528F94107" modified="2022-01-19" version="1">MKLSSAAITVAAISLLSTTHAAPLAFKNGEITGHLHHRRGSDISDISSASSASEAIDDLDIGFEPGATLLLPRIFNFFKRQSSSSAAISTSVSPSAQSTDDEATSGTTDTDLHTEDMTPDYESYGFASEGGDVSALAPVREVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKD8</accession>
    <name>A0A8H6CKD8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="5">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010263</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153935.1">
        <property type="nucleotide sequence ID" value="XM_037301118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKD8"/>
    <dbReference type="GeneID" id="59338655"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="6">
        <location>
            <begin position="85"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="430" mass="48166" checksum="9CA1EABABD0F7A54" modified="2022-01-19" version="1">MLSRALRGGHRALTLKRNVNVYVPLAKRTVTTDAASSHAEKEHVPEEDDKPFQVTLSNESFETYELDPPPFTLETTKKELKRMYYDMVSIRRMEMAADRLYKEKKIRGFCHLSTGQEAVAAGIEHAITKEDHVITAYRCHGFALMRGGTVKRIIGELLGRREGIAYGKGGSMHMFAKGFYGGNGIVGAQVPVGTGIAFANQYKDNTNTTICLYGDGASNQGQVFEAFNMAKLWNLPILFGCENNKYGMGTAANRAAALTEYYKRGQYIPGLKVNGMDILAVKAAVQYGKQYTTEGHGPLVLEYVTYRYGGHSMSDPGTTYRTREEIQRMRSTQDPIAGLKQKLLDWEVTTEEELKGIDKEARSSVDKEVEIAEKMAVPEATPKILYEDIYVRGSEPEFMRGRIPDENYYYEQSVREGGNLGRYEGERQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKE9</accession>
    <name>A0A8H6CKE9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010360</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154030.1">
        <property type="nucleotide sequence ID" value="XM_037301213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKE9"/>
    <dbReference type="GeneID" id="59338751"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF39">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="7">
        <location>
            <begin position="67"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="533"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="557"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="568" mass="62277" checksum="B6FB49A28F1B289B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKH5</accession>
    <name>A0A8H6CKH5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rad4-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010085</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153758.1">
        <property type="nucleotide sequence ID" value="XM_037300941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKH5"/>
    <dbReference type="GeneID" id="59338477"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2460:FF:000001">
        <property type="entry name" value="DNA repair protein Rad4 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.110">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2460">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018327">
        <property type="entry name" value="BHD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018325">
        <property type="entry name" value="Rad4/PNGase_transGLS-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018326">
        <property type="entry name" value="Rad4_beta-hairpin_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018328">
        <property type="entry name" value="Rad4_beta-hairpin_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042488">
        <property type="entry name" value="Rad4_BHD3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10403">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10404">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10405">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03835">
        <property type="entry name" value="Rad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01030">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01031">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01032">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="7">
        <location>
            <begin position="615"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="8">
        <location>
            <begin position="673"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="9">
        <location>
            <begin position="742"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="212"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="358"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="861"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="393"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="432"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="984"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1020"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1032"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1055"/>
            <end position="1066"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1089" mass="120507" checksum="6771D8D24DB1E6E1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKI8</accession>
    <name>A0A8H6CKI8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">W2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010205</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153877.1">
        <property type="nucleotide sequence ID" value="XM_037301060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKI8"/>
    <dbReference type="GeneID" id="59338597"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11561">
        <property type="entry name" value="W2_eIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000031">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="7">
        <location>
            <begin position="260"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="145"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="145"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="426" mass="47141" checksum="FAF9B7917CF6EDCE" modified="2022-01-19" version="1">MATLNIRRDVADPFYRYKMERLQSKIEGKGNGIKTVIVNLSSVAQSLARPPSYVIKYFGFELGAQTNTNPADDRWIINGAHEASKLQDYLDGFINKFVLCKMCKNPETEVKIKDGHITLDCKACGRRSDVDLRLKLSSFILKTQPRKGKKDKAEKKARRAMAKENGDADQNGNGHASPDGSNSDNADAEDGDLALEAGSDDELTRRINAEAKDLNGSDTKDDQWTVDVSEAAVKARAQELPDDFKQTLTLDGDDEEGDGEGGGNSAYDQLGSWVISTAQSKTGGVKDVEDVDIYIKAKELGIETKHKTLTVLAQTIFDDSIVEQIPTRASMLKKMITSERHEKAFLGGTERFVGNEHPKLVPQVPKILLAFYQQDLISEGVLKNWGSKASKKYVDLATSKKVRKSAEPFLEWLNTAESDEEESEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKM9</accession>
    <name>A0A8H6CKM9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2415 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153718.1">
        <property type="nucleotide sequence ID" value="XM_037300901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKM9"/>
    <dbReference type="GeneID" id="59338437"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF9">
        <property type="entry name" value="DUF2415 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="464" mass="51503" checksum="8CA260D9DCFAF19A" modified="2022-01-19" version="1">MNHASISPDGKLLLAVGDRHTAFFCKRVRLPSALSDGVSSYASYEWHEIAGLKLSLAESKDACFSTAFSPSGHICAVASQNGVITIFDTALIHDDMDAEEAVIESEDQGHVCVVDLRNAFQSRQTIDLHTDSPGLIRAEIEEYDTTSEQRQLEIERRFVERHREALEAQDQLAAVIRTANNVELGAERRRIERDAAAAQASRERQLIDSIGLRRLQESNSVSSGNLPTAPIRVNYNPSSTGRTESREWSGIPSPSSNPQTRSSASIHDFIRQRERTRLNDRSYQPRRRSSVVMSNSNSSSNTSSPHTSSLTPDTATPTLSASPSRLSASTSDAIATNTPFNPTDPWQTITDAMSSADIPPDTLRSLQSRNLERRVQATAVQQATLDRPNDLMMVEHADALEARGNRARDNVRSLRQMRAAHSRADVVYDEIDRELLLRRYQRSPRREAGLITMGIGWSEDGRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKN8</accession>
    <name>A0A8H6CKN8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010485</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154154.1">
        <property type="nucleotide sequence ID" value="XM_037301337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKN8"/>
    <dbReference type="GeneID" id="59338875"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052069">
        <property type="entry name" value="Ca-reg_mRNA-binding_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12962">
        <property type="entry name" value="CALCIUM-REGULATED HEAT STABLE PROTEIN CRHSP-24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12962:SF1">
        <property type="entry name" value="COLD SHOCK DOMAIN-CONTAINING PROTEIN CG9705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="4"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="80"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="161"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="239"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="316"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="470"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="519" mass="57372" checksum="65999ECC8DFA7CF8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKT0</accession>
    <name>A0A8H6CKT0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 15">Aurora kinase</fullName>
            <ecNumber evidence="1 15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_010221</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1 15"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153893.1">
        <property type="nucleotide sequence ID" value="XM_037301076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKT0"/>
    <dbReference type="GeneID" id="59338613"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044779">
        <property type="term" value="P:meiotic spindle checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902115">
        <property type="term" value="P:regulation of organelle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072479">
        <property type="term" value="P:response to mitotic cell cycle spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="141"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="88"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="219"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="268"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="12">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6225026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="432" mass="48494" checksum="00CA47FB63EF2587" modified="2022-01-19" version="1">MAIRAIESRFEQLSVTDENEPVNGGGTFHKSKGSFSSAMSISGLGSTTQLTSNPNRSNLLKIALQNNNDANTKAATITTVNVASQAAHWRGSTIPAPSSPPRKGASSSSSARPSDESAIQRLSNPPIYEQPQPKKFHLGMFEIGKPLGKGKFGRVYLAKERSTGFVCALKVLHKSELQQGKVEKQVRREIEIHSNLRHPNILRFYGHFHDSKRVFLILEFAGKGELYKHLRKENKFPEWKAAQYIAQMAAALKYLHKKHVMHRDIKPENILVGIHGEIKISDFGWSVHAPNNRRNTMCGTLDYLPPEMIKPGSQENFYNEKVDLWSLGVLTYEFLVGEAPFEDTPVMTQRRIARGEMTVPKFVSPEARDLIERLLVLDPEKRIPLVEVQKHPWIVKHCIKGERATQRNSASKETKLAIPAIIQAVNRTGLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKU8</accession>
    <name>A0A8H6CKU8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153923.1">
        <property type="nucleotide sequence ID" value="XM_037301106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKU8"/>
    <dbReference type="GeneID" id="59338643"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="487" mass="55217" checksum="EB01FF463A59E646" modified="2022-01-19" version="1">MASQNQPPILYDCYAVQPVDIVHEPPILQKLVVSQDPTPTEKLLISLQNIINQYSLRLNEVTKVDVSDNFDTLQDLCLSVERLQTEAQSSLDKRKADVKTVNRQHAACVTVLHDTIKSFRPASDVSPITRNIADCSKEIQAHLSTLTYAITDLKDHVDQLNSDLDRLRSREQQACLHRNRNIELTDKVVRLGLEAAELKGTKSDLYLQLQTSRNSGTVAPAAATRSKKAVKRVTPASKVTKKTSNAANKANKAPKAKKAPKATKAKTPPPREDILVHDTSSGTMSPDQVDETPPPTIKVDLQELIAQLSKVQEERNNRHPHEGRVHKSRRHHRRHSRSHRHRHSNTSNSDLFDSKSDWEERRGVPFRYIEDKKPFLSIAKRFRSVNVKYFKQIFFGTFKTKNFAKLAHIYTTPAKEDKDVNGHNHMMHCFHVYSVAAGRFAHVSVKEGLNEALHAYAIRLLRLPITYRFNSILAYHLAFVTARIQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLH2</accession>
    <name>A0A8H6CLH2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154291.1">
        <property type="nucleotide sequence ID" value="XM_037300443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLH2"/>
    <dbReference type="GeneID" id="59337977"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034333257" evidence="1">
        <location>
            <begin position="21"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="85" mass="9085" checksum="C3E796398902F435" modified="2022-01-19" version="1">MRATTLMSALLLLLISPAFAAPLVKRQGGVSAILDAAQQPDTEACTNGRRSLIPFWPRQYKAVNDCSFEIGSGSAAGATWRFDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLM5</accession>
    <name>A0A8H6CLM5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009795</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154502.1">
        <property type="nucleotide sequence ID" value="XM_037300654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLM5"/>
    <dbReference type="GeneID" id="59338190"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="14934" checksum="206078FF53782A15" modified="2022-01-19" version="1">MTSGIDLQALLSLTNSQYRINHSSPAGHPYDKIIVGARAANEKFFPNVFPIPGGHVADTDTEIFQTLVREVYKETALSFNPVKASTKPFAYATEKTVKQGEEKVVLQKSSLQLNFVCEVRKGTLRVNPEEHSEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLR2</accession>
    <name>A0A8H6CLR2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Seipin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009841</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154548.1">
        <property type="nucleotide sequence ID" value="XM_037300700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLR2"/>
    <dbReference type="GeneID" id="59338236"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23995">
        <property type="entry name" value="Seipin_BSCL2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009617">
        <property type="entry name" value="Seipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212">
        <property type="entry name" value="BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212:SF0">
        <property type="entry name" value="SEIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06775">
        <property type="entry name" value="Seipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="330"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="382"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="382"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="49733" checksum="93889852FF00E254" modified="2022-01-19" version="1">MTGLLMTGSSRPPERRHNPKKKIRERQTRLSNTSQNNDIHALKSMEDPKPRGRSGLIARAVDTALLPVHAAASKPAQRAYLGTFLFAATSILLFCVSSVAYTLFYYNYVPQNSVGRVVHLQFGDGHPHGTAHLSTSLTSLQPYDITLSLHLPRTPSNLAAGNFMLDLSLLGPSTTGTPSSILPSSLSSTTNTTTTIARSRRPAILTYTSAVVETANTLSGLPWYILGWKSESEVLSVSMFEGVEFAKGWANVPQSVRVVVEADEKMQFYEVGVRIIAKLGGLRWVLYHHRVLSFLFFTTAFWSSSMISMALAWFAISTYLGSPTTVKTEPETNGTIFKRERSDSDAFDPTSMEDLSDTSRTFPTLGRQIPLHFTGRKDVKKEEEIKREEDEVLRSTAVQPLAAEADDEDDDMSQETSGFRDSGIGTGLDDERLASVQRRRRALMNGKDSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMS2</accession>
    <name>A0A8H6CMS2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155083.1">
        <property type="nucleotide sequence ID" value="XM_037300257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMS2"/>
    <dbReference type="GeneID" id="59337791"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000043">
        <property type="entry name" value="General stress response phosphoprotein phosphatase Psr1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="439"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="613" mass="65315" checksum="519E776696A5B4C2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN96</accession>
    <name>A0A8H6CN96_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7624 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155095.1">
        <property type="nucleotide sequence ID" value="XM_037300269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN96"/>
    <dbReference type="GeneID" id="59337803"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056041">
        <property type="entry name" value="DUF7624"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24616">
        <property type="entry name" value="DUF7624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7624" evidence="2">
        <location>
            <begin position="608"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="563"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="886" mass="99057" checksum="337EF174C1D60C94" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPH2</accession>
    <name>A0A8H6CPH2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008660</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155526.1">
        <property type="nucleotide sequence ID" value="XM_037299525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPH2"/>
    <dbReference type="GeneID" id="59337055"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6227218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13672" checksum="535F6AF23F4DFACB" modified="2022-01-19" version="1">MVFILGNNFSEQRLVLKTLQGFYGIGPQISRHLLARLHIHPTARLSSLNSQTVLDINAELASMKIENDLRRELRENITRLRDMGTYRGRRHAMGMPVRGQRTRTQTATASKFNRVERLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPK6</accession>
    <name>A0A8H6CPK6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADP-ribosylation factor GTPase-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008735</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase-activating protein for the ADP ribosylation factor family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155600.1">
        <property type="nucleotide sequence ID" value="XM_037299599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPK6"/>
    <dbReference type="GeneID" id="59337130"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07608">
        <property type="entry name" value="BAR_ArfGAP_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000051">
        <property type="entry name" value="ARF GTPase activator (Csx2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000252">
        <property type="entry name" value="ARF GTPase activator (Csx2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000017">
        <property type="entry name" value="ARF GTPase activator (Csx2), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF160">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN EFFECTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="641"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="8">
        <location>
            <begin position="827"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="560"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="777"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1009"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1087"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="560"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="600"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1016"/>
            <end position="1028"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6227292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1119" mass="123901" checksum="00B2362F5EF6C800" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPY2</accession>
    <name>A0A8H6CPY2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Mevalonate kinase</fullName>
            <shortName evidence="19">MK</shortName>
            <ecNumber evidence="3 19">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_008628</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Mevalonate kinase; part of the second module of ergosterol biosynthesis pathway that includes the middle steps of the pathway. The second module is carried out in the vacuole and involves the formation of farnesyl diphosphate, which is also an important intermediate in the biosynthesis of ubiquinone, dolichol, heme and prenylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:17066"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="18 19">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 19"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155494.1">
        <property type="nucleotide sequence ID" value="XM_037299493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPY2"/>
    <dbReference type="GeneID" id="59337023"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000027">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000003">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 19" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 19" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="16 19" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13 19" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="15 19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="21">
        <location>
            <begin position="174"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="22">
        <location>
            <begin position="338"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU363087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAF6227186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="481" mass="51720" checksum="14818225D59D3A44" modified="2022-01-19" version="1">MASSNTKGTNATNGTLKGQSKSSKPRMERKISSPMNPAFMTSAPGKVIVYGEHAVVHGKAAIAAAISLRSYLLVTTLSKSQRTISLRFPDIGLSHTWDIDALPWSTFSHPSKKKMYYDLVTTLDPDLVKAMQPHLSDVSPEATGEHKKIRQAAASAFLYLFLSLGNQTFPGSIYALRSTLPIGAGLGSSASISVCLSSALLLQIRTLAGPHPDQPSEEAELQIERINRWAFVAEMCIHGNPSGVDNTVSSGGKAVLFKREDYTRAPKVTPLRKFPELPMLLVNTKQARSTATEVAKVGALKGSLPVITEAILNTIDAVTEAAYKLITSATFNQKDPHTAETLGKLMGVNHGQLVSLGVSHPRLERIRELVDLVGVGWTKLTGAGGGGSTITLLRPDASPKALKELEGKLDAEDFERFETTLGGDGVGVLWPAVLRDEGEEEGGVEIDQQKFLEAEGETGVESLVGVGAGREKREGWKFWRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPZ3</accession>
    <name>A0A8H6CPZ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155778.1">
        <property type="nucleotide sequence ID" value="XM_037299777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPZ3"/>
    <dbReference type="GeneID" id="59337309"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="128"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="407" mass="45348" checksum="848EA91F8392C47D" modified="2022-01-19" version="1">MGSWGWKLPGDESPELVLTERKSHDNVLGRHARLFHNHSSGALMLHLAKQSFAVVDGKKVSDSTTIWSDLTHITFGALSYQLQLDGANDSAHRGRLTRYQKIHGLTVNDYPVNLLSTPAKSDYIHKDYVIKNAIGHGSSSTVFAGEHIKSGNAVAIKRMTRTRQNAGPIEREIQMTAFLGRHHSLCHLIDGMSPNGDRRGQYFRNGAFDEVYLIYSPLVTTTWFPLCEPGPTSDAYYTICTKIFWQCLEGIDYIHSKGVMHRDIRPGNLAVVSWNPPQCRVINFGSAQMGINSNDVNVGTTPYCAPEMWRLSRSGKTSGLGYNEKVDMFAVGVSAYQIFCKQPLWWGHEADEYVLDVMKADLKSQGGAPLDVQDLIRSLLADDAELRPCAKDARKSLDNWHGPENQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQS6</accession>
    <name>A0A8H6CQS6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155826.1">
        <property type="nucleotide sequence ID" value="XM_037298490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQS6"/>
    <dbReference type="GeneID" id="59336017"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="683" mass="69290" checksum="A387483847FAD30B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQT1</accession>
    <name>A0A8H6CQT1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007682</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155888.1">
        <property type="nucleotide sequence ID" value="XM_037298552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQT1"/>
    <dbReference type="GeneID" id="59336079"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF7">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="234" mass="24867" checksum="7ADFBA3906B32314" modified="2022-01-19" version="1">MAHENRIHQLEDIANRSPQHEGKWDALAAGEMSVSSSHSGTTYLVPVPVTLKAQLGSPTALAIGAFSTTLTTLALALMEFRGLTITNVFIGNFFFVAGIGMVISAQWELVLGNTYAYTVLSAFGFFYGGFGAIITPLFGVQAAYGTDTAGYNNALGFFVLMQMAFTLVAASYFATADGHAGTALGLKKTAGAFAFLSGLLGYYTVANLMCQEAMFLSFPMGDTSRFFSAKKTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQT2</accession>
    <name>A0A8H6CQT2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007635</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155841.1">
        <property type="nucleotide sequence ID" value="XM_037298505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQT2"/>
    <dbReference type="GeneID" id="59336032"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05235">
        <property type="entry name" value="SDR_e1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010080">
        <property type="entry name" value="Thioester_reductase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01746">
        <property type="entry name" value="Thioester-redct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845:SF1">
        <property type="entry name" value="L-2-AMINOADIPATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="3">
        <location>
            <begin position="554"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1081" mass="120130" checksum="95033E90A3991408" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRF1</accession>
    <name>A0A8H6CRF1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Vacuolar protein sorting-associated protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_007948</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Component of the ESCRT-0 complex composed of HSE1 and VPS27.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Endosome membrane</location>
            <topology evidence="2 12">Peripheral membrane protein</topology>
            <orientation evidence="2 12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the VPS27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156152.1">
        <property type="nucleotide sequence ID" value="XM_037298816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRF1"/>
    <dbReference type="GeneID" id="59336345"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15735">
        <property type="entry name" value="FYVE_spVPS27p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21385">
        <property type="entry name" value="GAT_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16979">
        <property type="entry name" value="VHS_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1940:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000031">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000161">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017073">
        <property type="entry name" value="HGS/VPS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049425">
        <property type="entry name" value="Vps27_GAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21356">
        <property type="entry name" value="Vps27_GAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036956">
        <property type="entry name" value="Hrs_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0967">Endosome</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VHS" evidence="16">
        <location>
            <begin position="24"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="15">
        <location>
            <begin position="166"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="281"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="472"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="503"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="525"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="570"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="588"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="616"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="636"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR036956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6228218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="709" mass="79106" checksum="5F501EBA51711FD8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRJ8</accession>
    <name>A0A8H6CRJ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007997</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156201.1">
        <property type="nucleotide sequence ID" value="XM_037298865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRJ8"/>
    <dbReference type="GeneID" id="59336394"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="29"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="344" mass="38252" checksum="AA7570D5C96E4E61" modified="2022-01-19" version="1">MAEQQENRTPTIVATVTALWVLSYIAVGARFWGRFVGHSKLWWDDWLILLAAVMLVPALTADLIECHLGAGKHVQAVVQDHPGNIVNFLKSIYAGEIFYPPIIVCIKCSILTLYYRLFGIRKGIARVCHVLLGMTVAWGIATLLPAVFQCKPIHVAWDPTSTRNECFKLRPYLVGTNVPNVLLDCAILVTPLYPVWQLQLPRAKKVLISGIFVLGACETAFSIVRLVELTSLDISDVTWDYLPPIIWSTVEICVGMFCACLPVMAPLVPKFMRGRSGPKEPYDYSSFGPTNTARVTNGFAKQEFDRLEDDTAGLVLGRDHQLSIRQTTNIDVHEEGSIALHTMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRR2</accession>
    <name>A0A8H6CRR2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008087</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat UTP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156291.1">
        <property type="nucleotide sequence ID" value="XM_037298955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRR2"/>
    <dbReference type="GeneID" id="59336484"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000549">
        <property type="entry name" value="Small nucleolar ribonucleoprotein complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045161">
        <property type="entry name" value="Utp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359">
        <property type="entry name" value="WD-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="566"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="594" mass="65383" checksum="475143661F7ED7FC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F757</accession>
    <name>A0A8H6F757_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147001.1">
        <property type="nucleotide sequence ID" value="XM_037297565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F757"/>
    <dbReference type="GeneID" id="59335069"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="202" mass="22359" checksum="717EE66ACA955606" modified="2022-01-19" version="1">MKRALKMKDDDGSDLTTTDTTKSTKSKKARKEKLPDNVYKPGEAMPRPKYRGPYNQAHQDKLSAFSFGDAWKRRKSSGTVGSDLSPMGSRIMSRRNSAWSKKSKLGSRQNSAFAGVEESGEGDDDVANVGLSRQHTAENRRPRTNDITTDMAGLEFSKTITNGDVHGLDTQKTITNGRLFTEDELAKAMTQSTLKPLRQQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F769</accession>
    <name>A0A8H6F769_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006294</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147317.1">
        <property type="nucleotide sequence ID" value="XM_037297192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F769"/>
    <dbReference type="GeneID" id="59334695"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00845">
        <property type="entry name" value="TetX_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043683">
        <property type="entry name" value="TetX_monooxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46972:SF1">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46972">
        <property type="entry name" value="MONOOXYGENASE ASQM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="16"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6217882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="414" mass="46009" checksum="76553D659DB2F4C5" modified="2022-01-19" version="1">MASSSSTTTTTTTTLRIAIVGAGPGGLVLARLLHLASIPFVLYEAEPSRNSREQGGSLDLHEESGQLALQAAGLYSEWKEIARSEGEDMRVADKHGKLFMEEVDIEGRNRPEVDRIQLRGLLLDSLPEGAIQWGHKVRSISPSSNGNGKHEIHLRTTSNTRTEIFDLVVGADGAWSKVRPLLSDTKPHYSTISCLDTRIRSVDTLHPAISKLVGQGVYLAFSDKKGLVGQRNGDGSIRTYIMLQKPETWLKDVGVDWSDAIATKKYMLEEEYKDWDDELKSLITHANPDIVARPFYMLPTDFSWTSRQGLTLLGDAAHLMTPFAGEGVNLAMVDALDLSKAIVKASAEDEKTELFDAIKHFETTMLERSHEKMEETWRNLELFFKPDAPREFVQAFEEMMAAHGPPPEVPKGYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F870</accession>
    <name>A0A8H6F870_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-dependent RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005323</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RdRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148215.1">
        <property type="nucleotide sequence ID" value="XM_037296233.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333729"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.790">
        <property type="entry name" value="RNA-dependent RNA polymerase, slab domain, helical subdomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF14">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RDRP core" evidence="3">
        <location>
            <begin position="593"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="358"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1481" mass="166820" checksum="FE385DE61FDA9955" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F876</accession>
    <name>A0A8H6F876_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148021.1">
        <property type="nucleotide sequence ID" value="XM_037296840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F876"/>
    <dbReference type="GeneID" id="59334342"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF77">
        <property type="entry name" value="FI19380P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAS1" evidence="1">
        <location>
            <begin position="186"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="309" mass="32968" checksum="850FAABDB9D841E8" modified="2022-01-19" version="1">MYRYVALSLAMAATSMAQIPTNLTFHELTTQYLPFISLNTAYIFNDTRASQQLANAVDNPVTIFLSLNTPFQQFEATPPPSGAAGQIEAASVTSAALNSDLFQYLQLDGLHRSTSFNGTSFMRTRFNDTQYEHVTAGQNIEVFRNETAFYVGTGIGFKSNVLIPDLDFAGGIIHIVDHPMAPPYSLGNTGTVLSIDAIAGIIQASEKGPSPSFLPNIQNSGDVTVFAPQNSALNNISIASPQIAGNFVVSGPVLYSTALTAGTNFLTTGKTRLFVTTNANGAIFVNGSRIIESDILTANGVVHVIESPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8L6</accession>
    <name>A0A8H6F8L6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148650.1">
        <property type="nucleotide sequence ID" value="XM_037295952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8L6"/>
    <dbReference type="GeneID" id="59333446"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033988333" description="Expansin-like EG45 domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="2">
        <location>
            <begin position="59"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="185" mass="19490" checksum="A454D065FB304A26" modified="2022-01-19" version="1">MSSLALFTTLTLGGLKTLTMANPIAQQITPTTQPDGYQPGYSTQDWEPYGQQSACTINGGVCSAPTPLFPTTSGPDQLPYGVYAAAMVGLGPDDCTVCGTCYSIINSGTPYCDPNGPACPTGIYSGNEEQDGPSQIKILITNHCPDCAMINVTGQVQGHFDINLAPGGWDNAKIYYMQLDDSECT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8T8</accession>
    <name>A0A8H6F8T8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148967.1">
        <property type="nucleotide sequence ID" value="XM_037294920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8T8"/>
    <dbReference type="GeneID" id="59332410"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="564" mass="64866" checksum="B8BFF62A1A96E02A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F929</accession>
    <name>A0A8H6F929_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148951.1">
        <property type="nucleotide sequence ID" value="XM_037294904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F929"/>
    <dbReference type="GeneID" id="59332394"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="2">
        <location>
            <begin position="28"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="177" mass="19932" checksum="1CA30A204BCE0BB3" modified="2022-01-19" version="1">MVLMGHRGEDLQNLVAVEPSFWSAGAGLVAYFNFTNLQSDSHVGGAAFRAVIAQMLQARRSDPDFIDIASLLHDNGKGQNKALEQDILDIIRLFLKCQGPSFGVFDRLDECSDVEDLFLKFNDVPLKSSCKILILTRPIMDISGFIDHQRFHRVTLQNQKNSGDIEIYLRMRCGWVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA47</accession>
    <name>A0A8H6FA47_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150270.1">
        <property type="nucleotide sequence ID" value="XM_037293441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA47"/>
    <dbReference type="GeneID" id="59330928"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="73" mass="8635" checksum="E716ADE89D026D76" modified="2022-01-19" version="1">MNFTAWSVILHCPVSRGGPDIDPSEQKKIRHWIYRDYYLADEPESELVEWRAHIHGGRKMELPHQKHTSDLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA85</accession>
    <name>A0A8H6FA85_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003486</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149789.1">
        <property type="nucleotide sequence ID" value="XM_037294408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA85"/>
    <dbReference type="GeneID" id="59331897"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="482"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="510"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="97"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="594"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="649" mass="73308" checksum="5F83CD9C01ED0935" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAR7</accession>
    <name>A0A8H6FAR7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DnaJ homologue subfamily C member 28 conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150945.1">
        <property type="nucleotide sequence ID" value="XM_037293292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAR7"/>
    <dbReference type="GeneID" id="59330779"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018961">
        <property type="entry name" value="DnaJ_homolog_subfam-C_membr-28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394:SF1">
        <property type="entry name" value="DNAJ HOMOLOGUE SUBFAMILY C MEMBER 28 CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394">
        <property type="entry name" value="YALI0E31793P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09350">
        <property type="entry name" value="DJC28_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaJ homologue subfamily C member 28 conserved" evidence="2">
        <location>
            <begin position="250"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="565" mass="62753" checksum="8A0690BF9DFE2FB6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB68</accession>
    <name>A0A8H6FB68_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150809.1">
        <property type="nucleotide sequence ID" value="XM_037293156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB68"/>
    <dbReference type="GeneID" id="59330643"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="289" mass="32323" checksum="343BDAE346AE1BE2" modified="2022-01-19" version="1">MTSNSKEQSMSYVSRRGTGRNPSNPAIQSYLQDHEGSRMPQTIAQRSTLPRSQELQASSYPGPPPSDVGTSRLPARSDFTQDDSLPGLTHANSSRPSQSVSDGMGERQMNRALRIMIPDPDGALEPTTLPPLRRPDQGPVLECPFTFLKCFRQFAVSNEREWIQHSLEHFRIIDRRRSSRVDPPKNNSCCFCFQTFQASSGVDSWRDRMDHVKFHHALLGHRLAAARHDFALVEYLWQNGVLSLADYRELKPNAAAASRSTQNIPTPPDSPPGTGAVAVIHPNQRREGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBB4</accession>
    <name>A0A8H6FBB4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151048.1">
        <property type="nucleotide sequence ID" value="XM_037292509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBB4"/>
    <dbReference type="GeneID" id="59329995"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="149" mass="14682" checksum="6438AAFB8FB37E2B" modified="2022-01-19" version="1">MANSEQASCICFNTDGSWNGTVWDNAATTCYQAISSAGSANASALSAYDSGVVGFCTKFVDAGILSSAGVSSSTGVSSSAGASSGGGVVASTGQPSSTTGGGSGAVTGSSVSNPTRSSTQKSEVGHLKSQRSTVRYDHLKMRADVTDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBP4</accession>
    <name>A0A8H6FBP4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Galactose oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001941</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151408.1">
        <property type="nucleotide sequence ID" value="XM_037292869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBP4"/>
    <dbReference type="GeneID" id="59330355"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019760">
        <property type="term" value="P:glucosinolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47435">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47435:SF4">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="49519" checksum="8D43934A3F737AF0" modified="2022-01-19" version="1">MEPGIAAAAYGVETAVEGAAGAAVAIAKSTMPLKATWKRIPTDTILPRSSHSLSIIKSKAYIFGGEQQPREPVDNHVHVFTLQSANNDDVDYQVIRAKATTDEGAVPCPRVGHTASVVDDRIYVFGGRGGKAMKPLEENGRVWVFDTKMNQWSYLDPAKGSPYPAARSYHASTSTEHPVSPFQANTVQDPPPFNAAQEHTNIDQTVNPFGTRASGFDDHGTILIHGGCPTSGRVSDVWAFDIASRLWSQFPDAPGPARGGPSLTLSWERLYRFGGFDGEKELGGTIQFLRLTKSTFDVKGGKGELAVAPLTGQWESVEPPNGTPMPGDRSVAGLQPVTTGQGRHYLLLLLGERDPSSSGHEAAGRFWDDVWSFQLRPEGMTAASFKDATRQLFGAKTAEGTWARVDVAESSMSGNKEHPGQRGWFASAPAQDLDPGSVMLWGGVLSDNSRAGDGWILTVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBU1</accession>
    <name>A0A8H6FBU1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA biogenesis protein RRP5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ribosomal RNA-processing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_001165</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Involved in the biogenesis of rRNA. Required for the formation of 18S and 5.8S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151514.1">
        <property type="nucleotide sequence ID" value="XM_037292095.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59329581"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05702">
        <property type="entry name" value="S1_Rrp5_repeat_hs11_sc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05703">
        <property type="entry name" value="S1_Rrp5_repeat_hs12_sc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05693">
        <property type="entry name" value="S1_Rrp5_repeat_hs1_sc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05697">
        <property type="entry name" value="S1_Rrp5_repeat_hs5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05698">
        <property type="entry name" value="S1_Rrp5_repeat_hs6_sc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05706">
        <property type="entry name" value="S1_Rrp5_repeat_sc10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000103">
        <property type="entry name" value="protein RRP5 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000467">
        <property type="entry name" value="Putative rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000155">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000159">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000196">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000266">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000278">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000279">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057301">
        <property type="entry name" value="Rrp5_OB_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057302">
        <property type="entry name" value="Rrp5_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048058">
        <property type="entry name" value="Rrp5_S1_rpt_hs11_sc8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048059">
        <property type="entry name" value="Rrp5_S1_rpt_hs1_sc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24685">
        <property type="entry name" value="OB_RRP5_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23459">
        <property type="entry name" value="S1_RRP5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="136"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="249"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="445"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="536"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="630"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="718"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="814"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="923"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="1025"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="1117"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="1211"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="1300"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="62"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1385"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1425"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1458"/>
            <end position="1468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6222079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1798" mass="197412" checksum="002879B5766A0DAD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD27</accession>
    <name>A0A8H6FD27_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Imidazoleglycerol-phosphate dehydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152630.1">
        <property type="nucleotide sequence ID" value="XM_037291195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD27"/>
    <dbReference type="GeneID" id="59328674"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038882">
        <property type="entry name" value="Rcf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39153">
        <property type="entry name" value="AGR244WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39153:SF1">
        <property type="entry name" value="AGR244WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13302" checksum="BC82C9A551D580A0" modified="2022-01-19" version="1">MPRRTEEEVSAAGAEAYRGALVGAAKWGLIAGGLGAVGYFVSPIYRGLTVQFKVYIQLSGMTFGGWIEADRRLRAYEFRTRREKKRQNDEAVWKRWEGMIEQQERREQEQKQEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDG1</accession>
    <name>A0A8H6FDG1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_011029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153512.1">
        <property type="nucleotide sequence ID" value="XM_037301878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDG1"/>
    <dbReference type="GeneID" id="59339419"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="280" mass="32159" checksum="E55C86807186A878" modified="2022-01-19" version="1">MAAATQASPLLALPFELRTKVYEQLLRPDPDRVHTLYHDRHGREASFDFDPTILRVNKQIYSEAVSILYDTTNVRIHLATPVIMQCTGGNYPDHIVDPPDLFRKDAKGAVEPANRLDWRTTPSIVKELEIEGRLESTAHGFIYPHCFQRLHKIQLVTSRHAIWGDGECRSYFSHTGKTVLRILKLLAEEQATKPSMTKRLRFTIQPDWRTVESELLMRNGKMDNKTKVIVGLLKALERKTDAEIEVEEGGFKKTLRELKMEEVDVDEWEKVLLADADIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDX3</accession>
    <name>A0A8H6FDX3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_011088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153571.1">
        <property type="nucleotide sequence ID" value="XM_037301937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDX3"/>
    <dbReference type="GeneID" id="59339478"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="337" mass="37416" checksum="937A8085E8FFE392" modified="2022-01-19" version="1">MMQPTLNSLGGVSAIAQTLVQNLPLSPTQQEIRGLLFLFFGITFQANHQLNPATDNVNAYNNIINTFGMVNQVFTNPATNPYNIKPRYRCLEDYAVYYTHMTRSNGQQDFSLPLIGEYFDSVTEHGLTRPPPYNDIWPGLMYVNNQLDGTSAWHMPNLVSPSKGHLSQRSADLEDLHELNHLVTMVNYSTPPQVVPPDSNPPSTDAYFTNRDWPSWTFSTLYQSYGYFFAAFLGLTRLNADNWAIFGLCVSFPELDCLGSFRDDVRTGRSVPGGLSPGAVEKIREGGFDLTGDTPMDLDLERGPGGGGERAEGFDLEFAEKGESRFRDADVPAAIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEH1</accession>
    <name>A0A8H6FEH1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154226.1">
        <property type="nucleotide sequence ID" value="XM_037300378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEH1"/>
    <dbReference type="GeneID" id="59337912"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="850"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="386"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="480"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="515"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="649"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="690"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="760"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="773"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="793"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="858"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="893" mass="99843" checksum="E08CB0EBD920A311" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFB3</accession>
    <name>A0A8H6FFB3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009101</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154788.1">
        <property type="nucleotide sequence ID" value="XM_037299962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFB3"/>
    <dbReference type="GeneID" id="59337496"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17325">
        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF23">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="29"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6226235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="503" mass="53309" checksum="6D4931817D89DF18" modified="2022-01-19" version="1">MTRDILAWRSRDASKAPPLLKYRSSYSFILGTVSIAVFTDIFLYGIIVPVIPFALTTRVGVAEADVQHWVSVLIAVYGAALLGASPICGYVADHTSSRRLPLLIGLLALLGSTLLLCLGTTLALLIIGRLLQGFSAAVVWTVGLALLADTVDHEMIGQAMGYVSMSMSVAVLVAPLLGGVVYARAGYYSVFFMAFGLIVLDIILRVVLVEKKIAKQWVPAEPETSPPSPSKTLSEKATGPQTTMDQPEHTPSTASAPESSAPPLTIFPTKRQLPPVFTLLGSRRLLAALFCSLIQSTLLTAWDAVLPLYVHRLFGWGSTGGGLIFLPLILPSFVAPLIGYWSDKRGPRVPTTLGFLLAVPFLVAMRAVNHGGIDQIVLLCALLSCLGVALSMAMTPLLAEITYIINVKEKSHPGVFGRRGAYATAYGLFNTAFAGGMLVGPIWGGFVTQGSGWKTMGWTLAVLALVGAGVAALLIGGWVGRKSAMEVEAVDGGSDGRKEEGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFF2</accession>
    <name>A0A8H6FFF2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009373</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155060.1">
        <property type="nucleotide sequence ID" value="XM_037300234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFF2"/>
    <dbReference type="GeneID" id="59337768"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="527"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="270"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="717"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="735"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="750" mass="82452" checksum="317FEAA544203475" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFP1</accession>
    <name>A0A8H6FFP1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154697.1">
        <property type="nucleotide sequence ID" value="XM_037299871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFP1"/>
    <dbReference type="GeneID" id="59337405"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="74" mass="8603" checksum="4F290C6288D81B68" modified="2022-01-19" version="1">MATGTDPAKRLRSDLFTEHVDNTHSCQQVLKDNMHALDFLQFYRRTMARRLGKEKNSENLAGTQARYGQYLQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGB7</accession>
    <name>A0A8H6FGB7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bola-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155142.1">
        <property type="nucleotide sequence ID" value="XM_037299141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGB7"/>
    <dbReference type="GeneID" id="59336671"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044572">
        <property type="term" value="P:[4Fe-4S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230:SF7">
        <property type="entry name" value="BOLA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12183" checksum="D4890974D14DA943" modified="2022-01-19" version="1">MSTTPVEDAMRLKITAALQPTTLEIHNDSSMHAHHQAMQGSVSKETHFRVLITAEAFKSKMQPARHRIVYALLKEELERAGGIHALQLRTRTPEEEERQVAREKAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGC1</accession>
    <name>A0A8H6FGC1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155157.1">
        <property type="nucleotide sequence ID" value="XM_037299156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGC1"/>
    <dbReference type="GeneID" id="59336686"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="590"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="117"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="703" mass="79345" checksum="EC0EC4E12A51F88D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGG1</accession>
    <name>A0A8H6FGG1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008409</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155276.1">
        <property type="nucleotide sequence ID" value="XM_037299275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGG1"/>
    <dbReference type="GeneID" id="59336805"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="318" mass="33993" checksum="45F85D41C3012930" modified="2022-01-19" version="1">MRLGSASHSYTTLTSSNIPQGQSPPNSNSRGDYVTTRYTTQHPTYTLPLGFTNTEDFGPSGVVLVGPTTWATQDVLPVVEVANDLTTTSSTPQPWDANGFLDKNETVVASFISYGRQPACTSDFFSYAAESVDYCDSQMGYVFYLDQWPDYSGCKTSAGFDSTSTNAGTAARNQSVYATDSDGYIYTSPSIYVKFPTVSASDACGLIGSAATSITLAFSARELSTFVLNDGTGTNYQTFTSALDTAELPCGPWNGTDHFLPEAWSNYSSYQPLIVLPSKLIAMVPAWKSCTADMFEGQDPPRTLSPAAAMAPAPTNVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHU3</accession>
    <name>A0A8H6FHU3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">PRP1 splicing factor N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008139</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156343.1">
        <property type="nucleotide sequence ID" value="XM_037299007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHU3"/>
    <dbReference type="GeneID" id="59336536"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001164">
        <property type="entry name" value="mRNA splicing factor (Prp1/Zer1), putative (AFU_orthologue AFUA_2G06070)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000256">
        <property type="entry name" value="Probable pre-mRNA splicing factor prp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010491">
        <property type="entry name" value="PRP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06424">
        <property type="entry name" value="PRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="PRP1 splicing factor N-terminal" evidence="10">
        <location>
            <begin position="12"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="11">
        <location>
            <begin position="766"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6228409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="947" mass="105904" checksum="F092ACB130CC4F8C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKS4</accession>
    <name>A0A8H6FKS4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SNF2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004687</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157604.1">
        <property type="nucleotide sequence ID" value="XM_037295603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKS4"/>
    <dbReference type="GeneID" id="59333094"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052583">
        <property type="entry name" value="ATP-helicase/E3_Ub-Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SNF2 N-terminal" evidence="4">
        <location>
            <begin position="276"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6230347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="561" mass="62427" checksum="75316220A08C0877" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKS9</accession>
    <name>A0A8H6FKS9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Polyketide synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004691</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157608.1">
        <property type="nucleotide sequence ID" value="XM_037295607.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333098"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030918">
        <property type="entry name" value="PT_fungal_PKS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04532">
        <property type="entry name" value="PT_fungal_PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF37">
        <property type="entry name" value="SI:DKEY-61P9.11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22621">
        <property type="entry name" value="CurL-like_PKS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="6">
        <location>
            <begin position="8"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="7">
        <location>
            <begin position="921"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="1255"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="1373"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="4">
        <location>
            <begin position="921"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="4">
        <location>
            <begin position="1080"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="4">
        <location>
            <position position="953"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="4">
        <location>
            <position position="1141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6230351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1743" mass="191742" checksum="23D4DFC34D23B3C7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL14</accession>
    <name>A0A8H6FL14_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004828</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157741.1">
        <property type="nucleotide sequence ID" value="XM_037295740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL14"/>
    <dbReference type="GeneID" id="59333234"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF123">
        <property type="entry name" value="GMC OXIDOREDUCTASE (AFU_ORTHOLOGUE AFUA_2G01770)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="257"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="584" mass="62835" checksum="FC093596350AB6AA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYD3</accession>
    <name>A0A8H6KYD3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007180</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156708.1">
        <property type="nucleotide sequence ID" value="XM_037298073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYD3"/>
    <dbReference type="GeneID" id="59335580"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051609">
        <property type="entry name" value="NmrA/Isoflavone_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706:SF7">
        <property type="entry name" value="CIPA-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706">
        <property type="entry name" value="NMRA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="9"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6229066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="321" mass="35097" checksum="E9EC80A8A2359946" modified="2022-01-19" version="1">MATSNHIIKVAIVGAGGNSGSFMTEALLKTGKHTITAITRVDSQNKLPEGVIFKKVDYNKPETLVEALRGQDALVITLSNSVPKETEMQLINAAGEAGVAWILPNEWSPDTANEAMVKDVFLLQPKVDTRKAIADLGKSSYIAVSTGFWYEWGLAIPAGFGIDFANRIVTLFDEGETKISTSTRPQVGRAVGALLSLPIKPEGSNKEACLENFKNKVVYVKSFTVSQKDMLESALRVTGTKENDWTITKEPAQERYSSGIKEMQEGKRIGFAKMMITRVFYPDGCGDFEHNKGTLNSLLDLPQEDIDEATKAAIERSKTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYL9</accession>
    <name>A0A8H6KYL9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156872.1">
        <property type="nucleotide sequence ID" value="XM_037298237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYL9"/>
    <dbReference type="GeneID" id="59335745"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12108">
        <property type="entry name" value="Hr-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053206">
        <property type="entry name" value="Dimeric_xanthone_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38048">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38048:SF1">
        <property type="entry name" value="HEMERYTHRIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="1">
        <location>
            <begin position="27"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="386" mass="44718" checksum="58154CD6FAFC3447" modified="2022-01-19" version="1">MAGALPPLSPTDRDTYARMGTTMEWFHNNFRNTWKVLYGACSSGKRPANMSIRQFLNTGSEFAHHLTMHHTIEEQHIFPVLAQKMPAFKKELELLTQHKQIHNGLDKFEAYIDDCKAGKRDMRMNELKEIMDSFGTVLWAHLQDEVDQLSVDNMRKYWSLDEAKRFRNMWRKNGVGRLREDIVRDHNQDRVTLEREWETYNEHVRSMTADPEKRSADMEEEKSTLQRALQTPRALERDVEEMKLAKMALNIERDANAKNIVSQFRIERNQMVELQAKLDTKTAELLQEGKEATGKNSPDVSHGDLYAILSHHRRSQEPQELASVVEEGAEDDGEDLAGMLKRLLTSHARAVTEARIAKDRQRVLQQANKTIASGSLMRRHRTQLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYW0</accession>
    <name>A0A8H6KYW0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157007.1">
        <property type="nucleotide sequence ID" value="XM_037298372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYW0"/>
    <dbReference type="GeneID" id="59335880"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="1">
        <location>
            <begin position="74"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="173" mass="20140" checksum="8715E082A4EF274D" modified="2022-01-19" version="1">MKKVDQLARLYHVDFNLIIRKNEYDELSVDSLRRKEFSRRHYIWKTFTWDEKTQNLFAVAKKDEEKDRTVVTEDAIADVVEFVHESNRHGGWDATWKDISSSYYGILRSDVIRLLKECQTCALNPSKRPKGSAATMQSSQPIDHEFLDFLSTGDLQDDIPAWDVQEDGEHSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZJ9</accession>
    <name>A0A8H6KZJ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004363</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157282.1">
        <property type="nucleotide sequence ID" value="XM_037295281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZJ9"/>
    <dbReference type="GeneID" id="59332771"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000064">
        <property type="entry name" value="Lysine-specific histone demethylase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000249">
        <property type="entry name" value="Lysine-specific histone demethylase Aof2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF386">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="6">
        <location>
            <begin position="349"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="1118"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="1118"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="134"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="216"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1056"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1237"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1265" mass="139150" checksum="8C4503B4EE069714" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZM9</accession>
    <name>A0A8H6KZM9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004422</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157340.1">
        <property type="nucleotide sequence ID" value="XM_037295339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZM9"/>
    <dbReference type="GeneID" id="59332830"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22965">
        <property type="entry name" value="DD_DPY30_SDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049629">
        <property type="entry name" value="DPY30_SDC1_DD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6230083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="160" mass="16964" checksum="3D56C537A7B5F1FB" modified="2022-01-19" version="1">MAEETPKSPADAPDASTPIPPIDNIHSITDLPPPEPVLPPNAALSEPNLDTEMPEAAPPLPQMNYAPIPPTPIPATQRTATPTRHVNGTAAAEVAPPMPSKAASHGAPARRYLNEKVTGVLLEGMKRLAADQPENPLQVLGEFLLQRSRDLEGVKQESTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZU0</accession>
    <name>A0A8H6KZU0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">H/ACA ribonucleoprotein complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Nucleolar protein family A member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004606</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157523.1">
        <property type="nucleotide sequence ID" value="XM_037295522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZU0"/>
    <dbReference type="GeneID" id="59333013"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="108"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="242" mass="25926" checksum="3DAFF27399F6A98B" modified="2022-01-19" version="1">MAKEKPTDPEKEAKKLAKAEKKAKRSETNGVHKSKSDKKEKKEKKTAAATSDAEVTTKLLNTVEETKPGSVAVKEENGDVKVKVKMEPLLGALVPFANPLADEKAGKKVLKSVKKATKNKSLKRGVKEVVKSLRKSSTAAATAGSGDPPAIVILAADISPMDVISHIPVLCEDHGVPYIYVTSRAELGAAASTSRPTSVVMLTQEAVGKKKGGEEKGGEEEFKEVYRDLVKIVLKASRDVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZZ5</accession>
    <name>A0A8H6KZZ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Ribokinase</fullName>
            <shortName evidence="12">RK</shortName>
            <ecNumber evidence="2 12">2.7.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_004774</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose + ATP = D-ribose 5-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:47013"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="2 12"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157688.1">
        <property type="nucleotide sequence ID" value="XM_037295687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZZ5"/>
    <dbReference type="GeneID" id="59333180"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004747">
        <property type="term" value="F:ribokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01174">
        <property type="entry name" value="ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01987">
        <property type="entry name" value="Ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011877">
        <property type="entry name" value="Ribokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="14">
        <location>
            <begin position="5"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="41"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="241"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="273"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6230431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="341" mass="36605" checksum="3043F4CABBCAA343" modified="2022-01-19" version="1">MAVHTIAIIGGLVTDLLTIADRVPDRGETITSTRFSTHHGGKGANSAVAAYRLSHNNPKNSGHTPVMGGETQVRMIGAVGADEFGPPLKEKLTASGVNTDGIRVVKDQPTGVGVVLVEEDFGENRILFHPGANYDLLPSEFTTLASLAGGVKPDLLISQLELRRETIEQVIETASREGIDVLLNPAPASYLMPRIYRMVTHLIVNETEAVMLSPSDIDRQDGWANVGNYFLRLGVKNVVVTLGAQGAYYLNESGGGYVEAEKQCIVVDTTGAGDTFVGAFAAQYIQQMNTQGWDIKLAVEYACKASARTIEHVGCLDPIPWADEVNIPRASLDADKTKDMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5X5</accession>
    <name>A0A8H6C5X5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147057.1">
        <property type="nucleotide sequence ID" value="XM_037297621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5X5"/>
    <dbReference type="GeneID" id="59335125"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032157">
        <property type="entry name" value="PAC4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16093">
        <property type="entry name" value="PAC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="157" mass="16247" checksum="6BC93DB1E9E0C8C9" modified="2022-01-19" version="1">MSASSDAPSLPQPPSEPTATAATSASPTLHPQQLSVPLPLSPHTTLHVQITPLQTSTMVFLTTTDPSSTSSLSALGSFVYSMPNRLQPSEPLSTPLYAVPGSIDFATRVAKILARRTGLPAYVGCSAVFGGSVIEEEMAAVRLAVEGVMGVLGEGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6C3</accession>
    <name>A0A8H6C6C3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147177.1">
        <property type="nucleotide sequence ID" value="XM_037297467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6C3"/>
    <dbReference type="GeneID" id="59334970"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="213" mass="21273" checksum="D192DFFE55597758" modified="2022-01-19" version="1">MTVLAAPLKVVAGAVVEEVEEVEEEEEEEDEEDVVDAMEAVAGTVVELPTGKGAGVTVVEVAGGATGSGVVEVEKVEVVLVLVLLEVLEVDEVVEVVPAKEESIAIQRQGTVGADGEARGGRPLDWVVELELVVGDDFTGPALLIGEDTIFEGDDGVGGADGFALGGNGLGDVVGSDLEGAGVIFIAATGPLSGDGGGQEGEKGDGGEGLHGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6I9</accession>
    <name>A0A8H6C6I9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006332</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147355.1">
        <property type="nucleotide sequence ID" value="XM_037297230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6I9"/>
    <dbReference type="GeneID" id="59334733"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF39">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="52"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6217920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="489" mass="53457" checksum="ACF3E9C07154A03F" modified="2022-01-19" version="1">MSKREDEVSQGHTSPDALASLLRELKETLDYSIILTPDSEGYSDSIKRWSDEVEKRAGVVVYVGSAQDIASTILLSRKHSISFAVAGGKHSSSGAASTDGGLVIDLAKMRKVEIDTSKKIAKVQGGCIWQDVDEAAANHGLAMVGGTVNHTGVGGLTLGGGYGWLSGRYGLTIDSLLQVQIVLGDGSINTASENENPDLFWAVRGAGHCFGVVSEFVFETHEQKNNIWSGQMIWPASTKLGGVIDFANDLLTKSDPDSAMVLGVTAPPFMTEPAVVATVFHNGSQGAAERVFKPLLDLQPLKNSAQERPYREMNGMMNHAVPYGGRKLSKGACFVPPLSADYVRSLIVDMQKLHQTVPGSRRSIVLFEYLHPEAWCKIPNDATAFANRGRHQNMMIGPFWDNAEDDQKIRLWATEIAKKAEMELERVKEEAGSPAWMEHIGEYGNYDGLLTNARRIFGSNFDKLRSLKIRYDPDNVFDKSHSLLSAREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6V6</accession>
    <name>A0A8H6C6V6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006516</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147124.1">
        <property type="nucleotide sequence ID" value="XM_037297414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6V6"/>
    <dbReference type="GeneID" id="59334917"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034014937" evidence="2">
        <location>
            <begin position="20"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="262" mass="27919" checksum="FC0457C24A8B350C" modified="2022-01-19" version="1">MRSVYSTLLFLAAATPLLANEKSDCLAQASPTNGVIYWTGLEVPLDKADSYGNIMPGSNSTTKGCMQIGQWDGQIRIGKQPHPVKDTRTILETNPVGQWASYDVSYIPGFSYALACLDTVQQTLTGDNTDLFDVAGASCEDKNGYTCVGPVGAWGVRASEMSETSCWNCNPPNAFFAPVAGAAYVFPDDNGSVIAASSNAIHCCVGKHCPWKNPKAGTTKQGTCSCNSGADKRDATPETKPNTKRHLHSHGKHKVRGLGEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C724</accession>
    <name>A0A8H6C724_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sulfite oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006451</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147474.1">
        <property type="nucleotide sequence ID" value="XM_037297349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C724"/>
    <dbReference type="GeneID" id="59334852"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008482">
        <property type="term" value="F:sulfite oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02110">
        <property type="entry name" value="SO_family_Moco_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.420.10:FF:000002">
        <property type="entry name" value="sulfite oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005066">
        <property type="entry name" value="MoCF_OxRdtse_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008335">
        <property type="entry name" value="Mopterin_OxRdtase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372:SF6">
        <property type="entry name" value="SULFITE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372">
        <property type="entry name" value="SULFITE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03404">
        <property type="entry name" value="Mo-co_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00407">
        <property type="entry name" value="EUMOPTERIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase molybdopterin-binding" evidence="6">
        <location>
            <begin position="150"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Moybdenum cofactor oxidoreductase dimerisation" evidence="7">
        <location>
            <begin position="357"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="512"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="521"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="60733" checksum="C36BB4E61E0FAEAF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7E6</accession>
    <name>A0A8H6C7E6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Betaine lipid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147669.1">
        <property type="nucleotide sequence ID" value="XM_037297095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7E6"/>
    <dbReference type="GeneID" id="59334597"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021829">
        <property type="entry name" value="DUF3419"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47473">
        <property type="entry name" value="BTA1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47473:SF1">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11899">
        <property type="entry name" value="DUF3419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="851" mass="96140" checksum="ED0543AFBDAF1E53" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7W3</accession>
    <name>A0A8H6C7W3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcofluor white hypersensitive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147952.1">
        <property type="nucleotide sequence ID" value="XM_037296771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7W3"/>
    <dbReference type="GeneID" id="59334271"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="16576" checksum="681D28040C5B176B" modified="2022-01-19" version="1">MAGRRIVYVGLPVAAIGGYYFYAAGGEPKVAQKKAEHDAESAKASLKGQNISSAEAKKQGEEWAQIAGSKIDHTVDDVRNKVHDADKTISAKVSEAEAKFEKLKNDTASQFDKSKNDTKKEFNQSIDNFDKTVERKTAEAKSGISSWFGFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7W5</accession>
    <name>A0A8H6C7W5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147681.1">
        <property type="nucleotide sequence ID" value="XM_037297107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7W5"/>
    <dbReference type="GeneID" id="59334609"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034884263" description="Small secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="18591" checksum="26E374D4DD2FC1DF" modified="2022-01-19" version="1">MRLTAVTLANLSAFIVSASTLPKTPPAPSRLNITTIAAANDKSTLECWQLSAPFVQSSQAGTSGAVIAQLGETGATSYSLIPSQFDGGFHNAPVVQYVAFTSGLAIVSLPNSTRTATIPGGRNGLILATDTTNVSTLGHKTVYPSKEVTVGIQIPTRNNEIPLHTVLHAGPCTMGEMDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C853</accession>
    <name>A0A8H6C853_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006003</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148087.1">
        <property type="nucleotide sequence ID" value="XM_037296906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C853"/>
    <dbReference type="GeneID" id="59334408"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07981">
        <property type="entry name" value="HFD_TAF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000037">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037794">
        <property type="entry name" value="TAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003228">
        <property type="entry name" value="TFIID_TAF12_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264:SF21">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03847">
        <property type="entry name" value="TFIID_20kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 12" evidence="7">
        <location>
            <begin position="484"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="237"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="281"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="393"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="600" mass="65761" checksum="C2186CA3B2120F93" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Q9</accession>
    <name>A0A8H6C8Q9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148196.1">
        <property type="nucleotide sequence ID" value="XM_037296214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8Q9"/>
    <dbReference type="GeneID" id="59333709"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034246257" evidence="1">
        <location>
            <begin position="22"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="226" mass="23638" checksum="68E79F69C2D42CBA" modified="2022-01-19" version="1">MHLWSLSALVLTPTIRSVTFATPIVGPQSLPRNHVQCNAANELCQVQQTCDNAPSIVAGTVPKPWPSSVATLVNPVPALETLANGASGVSASRKLETRLQNTVQWCALGTEVYILLVLQGTQGAAAIKSTLGTAIRQVETQMDTQTANESETGIAAGSAPVYSLAGAVGSNAKITATNAAGVNMTWVELELGLLMIVDWMDNNSYGWGSASVWDGADEVGLVYITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C994</accession>
    <name>A0A8H6C994_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005153</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148763.1">
        <property type="nucleotide sequence ID" value="XM_037296065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C994"/>
    <dbReference type="GeneID" id="59333559"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="300" mass="34776" checksum="2C513AE4359179C2" modified="2022-01-19" version="1">MTSDRALGLDLSQRPRYSHPRKLAFRCFATVRSDIHHTGSPQDPFWPDLPLPNAVPTPYQIFRLKKDSPYSKRRFYELVKVYHPDKNDHESGSSDTSDLPGSIRMERYRLVVTAHEILSDPVKRSAYDKFGAGWNGQPDHGAPKYHWGQNNDKRWSGFDTNDSPFRNATWEDWEKWYQRDKAKQEPVYFSNGGFLMLVLAAVFLGGFGQSIRVGENMFQRQVEKVHDDASKAIRQRRTESQGFGNRDERLQSFLRTRDPHGYGVTDPLEESYRKLLQQPEICMSDVINQRGHDHGQESKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA20</accession>
    <name>A0A8H6CA20_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148791.1">
        <property type="nucleotide sequence ID" value="XM_037296093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA20"/>
    <dbReference type="GeneID" id="59333587"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="253" mass="28348" checksum="B2DC2DE367080C4F" modified="2022-01-19" version="1">MEGSFPSVNYPLHGGSPEASSNPPQASYPPMKTYQSLHLKQISSKAQILTFDQPHPAHPTYSIETTSTSFLGHKPDLAISREHNNISQKVAEGNFEKHNPVSKIKYRDLKKVEELRLEDHQTQRTIGTIDGMPWGWWQPSQVDNLLVEIVAPSNELIARFVYSNDHNFSDKVIKFGTVLGELQVDEQYASNQTVLDQVVCMTVTLVEREKRRQHKLRGEEGTAPVASLKVGITHPSNLEVDGPALPYDDGVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA35</accession>
    <name>A0A8H6CA35_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7703 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003925</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148894.1">
        <property type="nucleotide sequence ID" value="XM_037294847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA35"/>
    <dbReference type="GeneID" id="59332336"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056120">
        <property type="entry name" value="DUF7703"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37013:SF5">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37013">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_1G05950)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24802">
        <property type="entry name" value="DUF7703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7703" evidence="3">
        <location>
            <begin position="27"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="394" mass="44122" checksum="829F6F9A92E913FF" modified="2022-01-19" version="1">MPSPSAYDLAESASAKWRPGEELAASGFVAIVFYLFVDNNLSIYRIFKRKKGLYYWCMLLGTWGCLIDSIGVVLKYFVPNPEPLWPLYTLFLLGGWTIYAPAQLLVLYSRLHLVNQNRKLQRWVLIMIIAVASLTIIPTWPLVWQAFNPYKPRLSAIYSPREAIVDRFTQLGFTFAECILNGIYIWSLARVLNLMSGLRQRRVMTDLICVNVIAVCLDILTVVLVFLNQTGISHPIQTFSYILKLKLEFLVLNQLMAVAGGGPQKGIGADRHYHHPSTSVEKGASAASSKTSKWRLKSLLERAKAGSDEELMVPSPTLSQVQGRPDGTGSQITHDHADGGRWPVKAKVKERGAKGAVGIAEEENEEEENIGVHMWENRGKLAMEVSEFETECHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB16</accession>
    <name>A0A8H6CB16_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">uS12 prolyl 3,4-dihydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_003392</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3,4-dihydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54160"/>
            <dbReference type="Rhea" id="RHEA-COMP:13817"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
            <dbReference type="ChEBI" id="CHEBI:138052"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[ribosomal protein uS12]-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54156"/>
            <dbReference type="Rhea" id="RHEA-COMP:13816"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149695.1">
        <property type="nucleotide sequence ID" value="XM_037294314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB16"/>
    <dbReference type="GeneID" id="59331803"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031543">
        <property type="term" value="F:peptidyl-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009896">
        <property type="term" value="P:positive regulation of catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000014">
        <property type="entry name" value="Prolyl 3,4-dihydroxylase TPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.20">
        <property type="entry name" value="Oxoglutarate/iron-dependent oxygenase, C-terminal degradation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019601">
        <property type="entry name" value="Oxoglutarate/Fe-dep_Oase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043044">
        <property type="entry name" value="TPA1/Ofd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039558">
        <property type="entry name" value="TPA1/OFD1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051842">
        <property type="entry name" value="uS12_prolyl_hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117:SF0">
        <property type="entry name" value="PROLYL 3-HYDROXYLASE OGFOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13661">
        <property type="entry name" value="2OG-FeII_Oxy_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10637">
        <property type="entry name" value="Ofd1_CTDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="138"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="537"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="640"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="640"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="666"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6220260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="688" mass="76986" checksum="08D60DA934834FFB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB17</accession>
    <name>A0A8H6CB17_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149600.1">
        <property type="nucleotide sequence ID" value="XM_037294219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB17"/>
    <dbReference type="GeneID" id="59331707"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22997">
        <property type="entry name" value="GT_LH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36587">
        <property type="entry name" value="EXPRESSION SITE-ASSOCIATED GENE 3 (ESAG3)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EXPRESSION SITE-ASSOCIATED GENE 3 (ESAG3)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="537" mass="61476" checksum="1BCD9ACD74E86EBC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB61</accession>
    <name>A0A8H6CB61_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003861</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="59332272"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033986772" description="Peptidase A1 domain-containing protein" evidence="3">
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            <begin position="20"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="28"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="617" mass="65558" checksum="DB5BB1CBCEC67F04" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBW0</accession>
    <name>A0A8H6CBW0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150145.1">
        <property type="nucleotide sequence ID" value="XM_037294067.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59331555"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052897">
        <property type="entry name" value="Sec-Metab_Biosynth_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017:SF11">
        <property type="entry name" value="ESTERASE_LIPASE_THIOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="8"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="293" mass="30684" checksum="722EDA0223DC868A" modified="2022-01-19" version="1">MAATKPTILLIPGAWFHPSTYDAFLSRLQHLSFPTAYASYPSLNPSHPASADAAADTETVLKRSLLPLIETEGKDVVIVMHSYGGVPGSGAARGLSKVQRSGEGKKGGVVGLVHISGFVLPGGASVADGQGGELPGWVKQNEVPSTLARYHHHPSPPSRRLSARNPHSRSPSQPSPGLTMPANPLAILSADIDPPVAAANASRLVPHALLAFKSPAPAPAWTGPGFERRLAYLVCTEDEAIPKFGQEAMMQGTGVEWAVREMAGSHNAPFLKKADETAGAVDAFVEGFLKLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC08</accession>
    <name>A0A8H6CC08_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Membrane protein TMS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003048</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150050.1">
        <property type="nucleotide sequence ID" value="XM_037293972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC08"/>
    <dbReference type="GeneID" id="59331460"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="48233" checksum="C14C4A2827569A3F" modified="2022-01-19" version="1">MATRIAYAVILLLNSIISWLMLTPWALKKLEHLTLDYMAITCQGKQCYGFVAVQRVNFALGLFHFALAILLLGVKSSRDGRAALQNGYWGPKIIIWLAFVVMTFFIPEAFFQVWGKYFALAGAMMFVLLGLILLVDLAHTWAEFCLEKIENYDSKAWRGVLIGSTLGMYTMSFAMTIVMYIFFANSGCAMNQAAITVNLIVLLIISAISVHPVIQEYNPQAGLAQSAMVAVYCTYLTMSAVSMEPDDHHCNPLIRASGTRTASIVIGAIVTMLTIAYTTTRAATQGIALGSQTSHSYSKLGSEEAAEHGLVDTQPSASRREMRAEALRAAVEAGSLPANALDDDDEDEESNDGHGGKDDERRGTQYNYSLFHVIFLLATCWVATLLTQNIDFENAGDFAAVGRTYWASWVKIVSAWVAYGIYTWSLVAPVTMPDRFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCI3</accession>
    <name>A0A8H6CCI3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150206.1">
        <property type="nucleotide sequence ID" value="XM_037293377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCI3"/>
    <dbReference type="GeneID" id="59330864"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="82"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="221" mass="24674" checksum="6B40EB73ADB3ACA4" modified="2022-01-19" version="1">MESTLTLRLANEGDIDAMTTITTTAYPLDAQWRYRFPKLNVYSDDHHDFLRSRQAAHIANAMRGTQVVMLAEAPSNQNPSVKKVVALSVWQLPGTYMKEGNGSPKVQNQRRDADIPRMQAFKKSLGEAKEILLDRIYGENQLFLLSLATHPDHHRRGAGTMLCRWGMAKAREEGSTVTLFASVIGKMLYEKLGFKQVGMAHVQVDGEEEGLDIPAMVAGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCJ2</accession>
    <name>A0A8H6CCJ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150382.1">
        <property type="nucleotide sequence ID" value="XM_037293553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCJ2"/>
    <dbReference type="GeneID" id="59331040"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="251" mass="27546" checksum="B3EDF4D76C43D76E" modified="2022-01-19" version="1">MPPFRNPFNKKPPVVNGVPTIADENVRPSLGVRGDHASEKSSYTGSRASSSLSIKPRKEETAEYKLSVVNDSGVYLPPSPPEKKSFWQKSPTSTMSSNHRSMLNENEPFSISRESFDSYRRSFDISARSPVYSHESTAARQSLDSTMSRPSRSAVKSDSFSRPEPTTEDEEGFEDVGLNDEGKQPPKKKSIFARFGDNSETPVTGDAPRPTSSHRGFHLPGRKRGQSVGQGAELGNMDRPIANGNDDGVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCU7</accession>
    <name>A0A8H6CCU7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TATA element modulatory factor 1 TATA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002765</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150519.1">
        <property type="nucleotide sequence ID" value="XM_037293690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCU7"/>
    <dbReference type="GeneID" id="59331177"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052602">
        <property type="entry name" value="Growth_transcription_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022092">
        <property type="entry name" value="TMF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022091">
        <property type="entry name" value="TMF_TATA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515:SF1">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR TMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12329">
        <property type="entry name" value="TMF_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12325">
        <property type="entry name" value="TMF_TATA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA element modulatory factor 1 TATA binding" evidence="6">
        <location>
            <begin position="780"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="36"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="643"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="348"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="516"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="792"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="643"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="653"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="725"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="737"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="895" mass="99904" checksum="02B3A4E08DAA52C5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDE4</accession>
    <name>A0A8H6CDE4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Formin GTPase-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002288</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150867.1">
        <property type="nucleotide sequence ID" value="XM_037293214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDE4"/>
    <dbReference type="GeneID" id="59330701"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formin GTPase-binding" evidence="2">
        <location>
            <begin position="404"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="873"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="533"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="878"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="896"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="910"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="924" mass="102512" checksum="9D79DFF02107294A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDJ1</accession>
    <name>A0A8H6CDJ1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151060.1">
        <property type="nucleotide sequence ID" value="XM_037292521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDJ1"/>
    <dbReference type="GeneID" id="59330007"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="547" mass="61195" checksum="FCE80814A30D8A41" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDK8</accession>
    <name>A0A8H6CDK8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150909.1">
        <property type="nucleotide sequence ID" value="XM_037293256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDK8"/>
    <dbReference type="GeneID" id="59330743"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="172" mass="18608" checksum="EEDA298AE87FC005" modified="2022-01-19" version="1">MKGPPAGVNRPKESAEGLAEQQRRRPRDRATAEQSATSTAEPSRSQLGLRMGPDGLLRVDLLRVQPQSPGCDGQSAWDRPEQDRLADFKSQMAIPTGRPYAGMSYPEQRGSHTATLTSASVLALAETPKDKLFTELWPACSEKVYPGALNMDAIDSSVLCALATALDDRIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE10</accession>
    <name>A0A8H6CE10_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_001648</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151115.1">
        <property type="nucleotide sequence ID" value="XM_037292576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE10"/>
    <dbReference type="GeneID" id="59330062"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000024">
        <property type="entry name" value="MIP aquaporin (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF199">
        <property type="entry name" value="MIP17260P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="275"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="340"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6221680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="46566" checksum="B4605154C4296968" modified="2022-01-19" version="1">MNEGMEQPHVTRGFVLAGKTKATLAKGASLPFLGKPGNPTRNHFIAMCGEFAGTFLFLFFAFTGTQVANSQTQGSVSTTIAQGSNPAQLLYISLCFGFSLGVNAWVFFRISGGLFNPAVTFGMCLVGAMPWVRGGLIFISQIIGGITAAGIVEVMYPGQLNVRTSLGAGTSKAQGVFIEMFLTTMLVFTIFMLAAEKSKATFVAPVGIGLSLFIAELAGVYYTGGSLNPARSFGPDVILGTFEDYHWIYWIGPLLGALLASGFYKLMKGLEYETANPGQDFDENETKLFDPERDIERPIVNVVTGGSSSEPSRPISGDTLSGYKREDTSPQQDQPRDVAQGNSRHQAQSSAGATRSPRRLSSPLPPIDAEDEKSIAPPDNRDHSNTNGVSGEDIIHGGPHYSQTANDLDPRVRREIDAVGTYQAGPDVEAGQSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE38</accession>
    <name>A0A8H6CE38_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151345.1">
        <property type="nucleotide sequence ID" value="XM_037292806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE38"/>
    <dbReference type="GeneID" id="59330292"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="247" mass="26843" checksum="2F0AFF7A995B9AE7" modified="2022-01-19" version="1">MSHSTYQQRDFATVKANWLAEELSDGRISWTDYLSRTVPVMVGVQSQGKTVECLPVVIALSEFFEVLAHDLRNMFLSDSNDTNVQHEQAILKVKWGGSGSQQFDGYDTMLLSSKNLEPTIRLLKQRQGTDMILLELNGWYSAGQVDPIGGQKVLRREGRKKQGGRIAPEGESGEKRNSGSHSDVSMEGTSPPKDVPMMEGRADIQPDSGIQQSAGRSRDWVKESSSQAARGKGGGQGGGGKSGKSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEL5</accession>
    <name>A0A8H6CEL5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001933</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151400.1">
        <property type="nucleotide sequence ID" value="XM_037292861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEL5"/>
    <dbReference type="GeneID" id="59330347"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="340" mass="37287" checksum="ACB2AA82AA44046A" modified="2022-01-19" version="1">MSRVLLTGGSGFIAAHVLDILLERGHSVVTTVRSTEKGQKILDNHPKVSKDKLDFVIVEDIAKPGAFDKAVQSNPPFEVIIHTASPFHFNATDTKKELLDPAVIGTTGILESTKNHAPTVKRVVITSSFASIIDPSKGTRPGYTYSEKDWNPITEEEAVQNPSNGYRASKTFAERASWEFLEKEKPNFTISTMCPPLVLGPIVHYLQSLDNLNTSNQRIAALMTGKAKEELPPTGTFVWTDVRDLALAHVKAAELPEAANKRFFVTTGYFSNEEIADIMRDNFPELKKQLPAKGTKGGGYPEGGLYQYDNSRVKEVLGIEFISLKESIVDAVKSLQAVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CER7</accession>
    <name>A0A8H6CER7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001238</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151587.1">
        <property type="nucleotide sequence ID" value="XM_037292168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CER7"/>
    <dbReference type="GeneID" id="59329654"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF2">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="508" mass="58296" checksum="81E87E06A565BFEA" modified="2022-01-19" version="1">MGFVRSLIKRIPSTPALPSLEDLSNEKDRFAPRLSFFKRRIRLKGNSKISVPLGAVLLFPCIVIILIVVLIVRHPSSPGGLMMPAGAPPAIRKISEQYDKVFVTGCLEPQTNGPRANAVLVILARNQELEGVVQSIKSMERHFNRWFHYPYVFLNDGDFNTTFKETILNHTSAPVEFGKIDPTMWGYPDWVNIDTAREGIRKQGDAAIMYGGMESYHHMCRFYSGFFYKHELLQKYDWYWRIEPEIKYFCDITYDPFIHMERANKTYGFTIAVKELKETVPNIFRYASAYKRVNNLTSQGLWEMFAEPGSAAEDISLPEDNPKYKKPLPEEILSSEPGQGGVPDVDPEAMEGETYNMCHFWSNFEIARLDWFRSKEYNDFFDMMDRSGGFWMERWGDAPIHSLAAGALLAPQNIHYFRDIGYRHTTIQHCPANAPGNRQLARPPFLEVTTKDEKKRKEEDEYWETWDPVKENGVGCRCKCDTDIVDVEGKQGSCLDEWVNVAGGWASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEW2</accession>
    <name>A0A8H6CEW2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151735.1">
        <property type="nucleotide sequence ID" value="XM_037292316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEW2"/>
    <dbReference type="GeneID" id="59329802"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="282" mass="31728" checksum="18ECCD440E6EC2F6" modified="2022-01-19" version="1">MTKPRYPNEDATQGPFSFGASGFRINDTFRESGKCLKRLLFPESLKSEKARKSARNDASMIDRPWIKAQLQHYGIDFNLNIDPLKAKALLLISVAYGLCDAVPPQVLKIKSTLKEQYKSIIEDYRDRIKANAENTDDESDSEDMSGDKEDSDDERAAGRSKDSDYNTGGEPDNPIDTALDAPVKRQKIESSHPRRLYFQWRGYNTMLGAVQFDPQGRNTGYLDFANDDATIFEGNILMDAMGGEILFQGYGVPGLSGPLTMNWNALSHLAAERAKVQYYMWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFD8</accession>
    <name>A0A8H6CFD8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="2">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_001441</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151790.1">
        <property type="nucleotide sequence ID" value="XM_037292371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFD8"/>
    <dbReference type="GeneID" id="59329857"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="120"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="12">
        <location>
            <begin position="516"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6222355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="584" mass="65830" checksum="707A24D096C2D020" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFW3</accession>
    <name>A0A8H6CFW3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaminase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000672</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151971.1">
        <property type="nucleotide sequence ID" value="XM_037291609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFW3"/>
    <dbReference type="GeneID" id="59329090"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052743">
        <property type="entry name" value="Glutaminase_GtaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032514">
        <property type="entry name" value="GtaA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033433">
        <property type="entry name" value="GtaA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987">
        <property type="entry name" value="GLUTAMINASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987:SF12">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17168">
        <property type="entry name" value="DUF5127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16335">
        <property type="entry name" value="GtaA_6_Hairpin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminase A N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminase A central" evidence="1">
        <location>
            <begin position="243"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminase A central" evidence="1">
        <location>
            <begin position="421"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminase A central" evidence="1">
        <location>
            <begin position="575"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="717" mass="79988" checksum="F1CC5B45CD6CB6AA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGK0</accession>
    <name>A0A8H6CGK0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152142.1">
        <property type="nucleotide sequence ID" value="XM_037291780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGK0"/>
    <dbReference type="GeneID" id="59329263"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="277" mass="31397" checksum="6148AD42B76E24F3" modified="2022-01-19" version="1">MSGWERRSSLPVNSNGPMITENEHARRRNITEVICISEEPQADLQREGFRKAPDLHSIIFTLKSDTEGDLLSIFGKVCDAIDRTLWEGAGVLVWDVGGGVAALAAYRKYSITCAPTSSTVHVIDRTTATVMKKHELCLPEAMTTITRARLMRPKPTLLPSQRIIDLLHNDVYQGQLEMWQACEYDTNENTIVNPTEPREKWIVEGVGGSPKKKRVNVQSTLRRLDVVQSVLKRPSDVQSVLRRLDERLPHTSEHLHRLSTIEEVEEEARRLSIILEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH30</accession>
    <name>A0A8H6CH30_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alcohol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000250</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152625.1">
        <property type="nucleotide sequence ID" value="XM_037291190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH30"/>
    <dbReference type="GeneID" id="59328669"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05188">
        <property type="entry name" value="MDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF4">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="3">
        <location>
            <begin position="33"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="2">
        <location>
            <begin position="197"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="39379" checksum="83EB424E97BA3568" modified="2022-01-19" version="1">MTTQTLPQAHRALILNSTSSPPAIQTIPTPQPGPGSAVVRILAANVISYMRQIYNGERQYPFPTPLVPGTSAIGRVAAVGPDATLLKPGMLVLVDCTIRGRDDATAVFLSGIHEGYSEGSKTLMRGEWRDSTYAEYAKVPLETCEVLDEGKLCGRMGYEIADLAYISALLVPYGGLRDIDLKAGETVIVSPATGAFGGAAVLVALAMGARVIAMGRNVETLKKIKKRCERVEIVPITGDVLKDSEALKSFGAVDAFFDISPPEAGKSTHIKSGILALKHSGRVSLMGGIKDVSIPHSVVIHRNLTLKGKWMFEREDIKGMIKMVENGVLKLGESSGSRIVGKFGLEDWDKAFTAAEKNAGMGESTLITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI26</accession>
    <name>A0A8H6CI26_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">U-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_010554</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ubiquitin conjugation factor E4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153040.1">
        <property type="nucleotide sequence ID" value="XM_037301406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI26"/>
    <dbReference type="GeneID" id="59338944"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16657">
        <property type="entry name" value="RING-Ubox_UBE4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000055">
        <property type="entry name" value="Ubiquitin conjugation factor e4 a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="12">
        <location>
            <begin position="997"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6223980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1084" mass="122605" checksum="42DF6FA2960A7AA4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI39</accession>
    <name>A0A8H6CI39_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152979.1">
        <property type="nucleotide sequence ID" value="XM_037301345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI39"/>
    <dbReference type="GeneID" id="59338883"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="700"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="732" mass="80788" checksum="931CC8FB1251186E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CID7</accession>
    <name>A0A8H6CID7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HotDog ACOT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010592</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153078.1">
        <property type="nucleotide sequence ID" value="XM_037301444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CID7"/>
    <dbReference type="GeneID" id="59338982"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000032">
        <property type="entry name" value="Acyl-CoA thioester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000038">
        <property type="entry name" value="Acyl-CoA thioester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="6">
        <location>
            <begin position="48"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="6">
        <location>
            <begin position="245"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="430" mass="48356" checksum="356113AF549FD410" modified="2022-01-19" version="1">MRVRTPWIEALEKQRKEGFDPTKPSSTPATPPDRDLTPKKMSDSFHRVVIPLAQDPWLLDNYLNSNGHIRLGTIFMDLDALSGVIAYKHTGESVTTVTAAVDRITINHPLKELCDLELSGQVTYATGRSSMEISLQVAKAPEKGAKVHKEDVLMTCAFTMVSLDPSTKKPVAISPIKLETEEEKRLFELGQKNSTGKKALAKRTLRKQAPNEEESELIHAMWLKQLEYHDPNISTRKPENVIYMDKTRQQSSQIMQPQYRNRHNFMIFGGFLLKQTFELAFCCAAACSHSRPTFVSLDPSTFENPVPVGSVLYLTAIVAYTDPPLVTTNIAGDGVEDTAEGPGERYTRVQIRIDSKVRNVEHGETKPTGQFNYTFLVERNIRVMPSTYSEFMIWVDARRRAESVKASIEGLEPGRLRNGTDDGRSLRVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIJ5</accession>
    <name>A0A8H6CIJ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010594</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153080.1">
        <property type="nucleotide sequence ID" value="XM_037301446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIJ5"/>
    <dbReference type="GeneID" id="59338984"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848:SF4">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="234" mass="25854" checksum="197231110CA6B4D1" modified="2022-01-19" version="1">MGKSRSTALLIAYLLSQNPQLNPQSALDLIRQSRDFAEPNSGFMEQLSLYHNMGCPATQEALDNHPLYQRWLYQRTVEASVAAGVAPEVSELRFGDEGNAMPMNLAAEEGPANLKSSGYPSEPTTSYRCRRCRTPLATSVYLVPHNPKRPATSQPCAHLHLTPLSWMRPELEQGKLEGRLECPNQKCGQSVGRYAWQGMKCSCGEWVVPGMTLGRGRIDEVRERPRAGIGGGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIN2</accession>
    <name>A0A8H6CIN2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010488</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152974.1">
        <property type="nucleotide sequence ID" value="XM_037301340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIN2"/>
    <dbReference type="GeneID" id="59338878"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="106"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25839" checksum="325A007F4F9BC80A" modified="2022-01-19" version="1">MSTSNLKPLILHAHNTGPNPYKVAIVLSALKVPYTVKLWEFGDAPNGVKGPNFLKINENGRVPALEDPNTGVVSWESGAVIHYLLRVYDKSNKLGPSGKSPGSEPTNQDHVDFNKWEFFLVSSLGPMMGQVNWYRHYNPTKNEDALSRYEAQTYRTFDVLEGQLRKSGGESVLPGGFNAVDAHFYPWVNQYTYAELKLDKYPKIQKWLEVIGKIPAVLEAYERVPNGEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ17</accession>
    <name>A0A8H6CJ17_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010991</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153474.1">
        <property type="nucleotide sequence ID" value="XM_037301840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ17"/>
    <dbReference type="GeneID" id="59339381"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="502" mass="55255" checksum="7A40E14CE49487CE" modified="2022-01-19" version="1">MNATDHAELYVKYKSFLGIDNHDSSVSQLLARDSAQNGVLSSGHGLFCPAEAIIRGSSTQFEDSILEVAVFENPADCADDRKRQHGAESILKDELHESMKKHLRPFEDTVSESLSNMNPLIKNIPEIYRPSDSSNAPDGSTSSIVFFGLHLFVDSYKSVTDKISSSNNNEHGDCFNQATPHRGLYPAREQDCVNAAKELFYFRDPFRPTTFARRNNVGFRLPKVVRNGTCVISIDVMNDADKDKFKPWLVYTTAMDIAHRCTQAAFGFGGRSMAGPKKVVDVLVFGRGLPLKNGAVGPAVSESAVIVAREQLARDDSSLLNETSLNLIKPSAVDTTSLDDRLGVNAPQLGGALVCADPPLPRERAWPINFKDCEMATNAVFGDRQRDQRYTFSREPVATKFYYPLPAKVRYRSCVILLDMNNNNDQDTVRLSIVEATAWVLAHKCSGEERSVDQFGGRATVGVGAKDLINIWVYGSLWPTPDGATNVTGLVLAQPARLIDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ50</accession>
    <name>A0A8H6CJ50_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010974</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153457.1">
        <property type="nucleotide sequence ID" value="XM_037301823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ50"/>
    <dbReference type="GeneID" id="59339364"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000055">
        <property type="entry name" value="TBC domain-containing protein C1778.09"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000023">
        <property type="entry name" value="TBC domain-containing protein C1778.09"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="515"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="339"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="785" mass="87209" checksum="91553BB30B15C533" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJB0</accession>
    <name>A0A8H6CJB0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153304.1">
        <property type="nucleotide sequence ID" value="XM_037301670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJB0"/>
    <dbReference type="GeneID" id="59339209"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886:SF1">
        <property type="entry name" value="NACHT-NTPASE AND P-LOOP NTPASES N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886">
        <property type="entry name" value="SESA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="140" mass="15044" checksum="07E5DBC2ED7A308D" modified="2022-01-19" version="1">MTPAFGFSVGDLISAIGLVKRVTKALKETGGAPTDYQLVVIELKGLKSILRHLETLKPTKDNVTHINAIGGMALACQLPLRDFMIASSDGEEVERLRAVVAAKPISLNLLLAMNTSQTLTPMDSPDQEKHSDLMSMAAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKL6</accession>
    <name>A0A8H6CKL6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153922.1">
        <property type="nucleotide sequence ID" value="XM_037301105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKL6"/>
    <dbReference type="GeneID" id="59338642"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF14">
        <property type="entry name" value="RETROTRANSPOSON COPIA-LIKE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="33050" checksum="3D8EFB505C1DE8DE" modified="2022-01-19" version="1">MDPETSSFKSPIRQLTDNNYSEWLIDIKALLRGKKLWNYTQEAPDSDKSVTAQAKWRNSAMEAADLITPTISGPVKQKLQANAFDDGYLMMNSLANIYAPKGDAEFMRLTREYYSLRYEDFDSMTHYLTHIKTLEERIRGTNVVLDDDKQTLLCLGMTLPEGFQYFTKIWAMTPGMTADKARNMLLEEERRQKTLDIGTVGTGGVGLVAITRHPSKRTPSGKAIVNDVVCPRCGKGHEADICWKLHPELAPEWLQERWTIEKRSRKRKWDELQQQDQEAPGEEGAFINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL37</accession>
    <name>A0A8H6CL37_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154086.1">
        <property type="nucleotide sequence ID" value="XM_037301269.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338807"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23242">
        <property type="entry name" value="TRANSCRIPTION FACTOR HOXA13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23242:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR HOXA13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="521"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="617"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="678"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1519" mass="159004" checksum="AB39590F99836631" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL44</accession>
    <name>A0A8H6CL44_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154167.1">
        <property type="nucleotide sequence ID" value="XM_037300319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL44"/>
    <dbReference type="GeneID" id="59337853"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="96" mass="9955" checksum="B356092D5283EE20" modified="2022-01-19" version="1">MLRKRPLHIRVAEIVPPPPLEHGLFVPAPGFDENRVEAAEELPGGPGHDGEDGGVHGGEPFLDGAVGEDEFGVGVGAHEFVDHAGGGEVHDGLVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL95</accession>
    <name>A0A8H6CL95_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009602</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154311.1">
        <property type="nucleotide sequence ID" value="XM_037300463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL95"/>
    <dbReference type="GeneID" id="59337997"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF47">
        <property type="entry name" value="ENDOPEPTIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01220)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="270"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="8">
        <location>
            <begin position="468"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="484"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="833" mass="89962" checksum="823466B9D0F873B3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLD6</accession>
    <name>A0A8H6CLD6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GST N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154382.1">
        <property type="nucleotide sequence ID" value="XM_037300534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLD6"/>
    <dbReference type="GeneID" id="59338068"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058268">
        <property type="entry name" value="DUF7962"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25907">
        <property type="entry name" value="DUF7962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="10"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7962" evidence="2">
        <location>
            <begin position="113"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="36604" checksum="11DCEFE087B56098" modified="2022-01-19" version="1">MAPHNDIVFFHYSFSPFARKVIWYLTLRGIEYGQCDQIQPVYLPREDINALGVKYRRIPIMSIGRDIYCDTRLILHKLEEKFPSGALGAPQPDQKAIEKLLESWTIDGGIFVRAAQAIPTDMPLLNDPKFTKDREDYTGRSWDKKNIEAMRPEALTHIRDAFVFLETGLLADGRQWVLKTEKPSLADIEGLGALPHSLISKEKFPKVYAWIGRFSKAVSTAKSSAPKPITLKGAEAVKHVTQAEFYEPEGDVDASDPLGLKKGQDVESWPIDSGFKHHDRGQLVSLTSKEVVLEAQSKVGGRDVRIHHPRWNYRTRAVSGGEAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM19</accession>
    <name>A0A8H6CM19_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009717</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154425.1">
        <property type="nucleotide sequence ID" value="XM_037300577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM19"/>
    <dbReference type="GeneID" id="59338112"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051122">
        <property type="entry name" value="SDR_DHRS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057571">
        <property type="entry name" value="SDR_PhqE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477:SF1">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23441">
        <property type="entry name" value="SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="27940" checksum="0A164F9E2D84823F" modified="2022-01-19" version="1">MADSKKYSNKLAGARVLVIGGSSGIGYCVAEACLEFGASVTISSSQQSRIDSSIDQLLTTYPSAKGRIAGYACDLSSPGVEANIEKLFERCGGPKFDHIVHTAGDQLAMLKLEDATLESIQKAAMVRFNSPLLLAKHAPKHLNPGPASSITLTTGSVSRKPNKDWSIVASYATGLHGMMRSLALDLAPVRVNLISPGAVLTPLWDGMAKEKMESFMEHIKGKCTTGEIGRPEDVAESYLYVMRDWNVSGSVIDTNGGFLLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMD7</accession>
    <name>A0A8H6CMD7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154628.1">
        <property type="nucleotide sequence ID" value="XM_037299802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMD7"/>
    <dbReference type="GeneID" id="59337334"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="216" mass="23359" checksum="27A3BBEA292EC46D" modified="2022-01-19" version="1">MSLNDGTSGSITILSGQIDLEAMVSSLAMLGIGCTLLASSLALPPNRMIATSVPKPAGRSVSSRAGNLDPLTLLDLHLNTSITTPRPSESPVGSWAIECGRDNTPPGWAVPPETHQIARPTDCRSAISRVIRGGEPWEPQIWTSQANWSFRSCGVFLAPGWSYARVNFPRIDMAEIAQEIARMCVTQEHDFMGGWVAIGGYFILLLTGREAPERQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMJ7</accession>
    <name>A0A8H6CMJ7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154703.1">
        <property type="nucleotide sequence ID" value="XM_037299877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMJ7"/>
    <dbReference type="GeneID" id="59337411"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="286" mass="34311" checksum="D2F0DF550EBF639A" modified="2022-01-19" version="1">MEVSSASHQQRPFPFMSLPLELRRKVYYFAVSRTRPIKLRINYHGLATTDSLGPGRVRMYTNQDFRMLETNIEFRKELSDALYAKSTFDLSLWWQETEKGTRLFQIDPRRIRICRLFIHNLANTIYTPHRDVWGGAFPLYWHHHLRAFVATLVFNGHQIESMLVECEEQDSNWLLECLRPMAMLRNFNLVHFRSCRREVHPYFRFLEAYMMSDRDVPFRDWQGFRKQTEPWRPHFLSEEARRVPPPRSTDMTDEGVTKSKEEMEATARTLYAILQIEDEMRPQQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMX2</accession>
    <name>A0A8H6CMX2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154898.1">
        <property type="nucleotide sequence ID" value="XM_037300072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMX2"/>
    <dbReference type="GeneID" id="59337606"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF102">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13501" checksum="6FE60FE6BEF38534" modified="2022-01-19" version="1">MPEIEDPTQRFVSVVKFYLSGWHIKPPGVKKPLNPILGETFTCYWDYPDHTRGYYISEQTSHHPPKSSYFFMAPEHNIRIDGALKPRSKFLGNSAASLMEGVGVLRFMNRGRTQGGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMX6</accession>
    <name>A0A8H6CMX6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase ROK1</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP-dependent RNA helicase rok1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_009350</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the U3 snoRNA and is associated with the 90S and 40S pre-ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155037.1">
        <property type="nucleotide sequence ID" value="XM_037300211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMX6"/>
    <dbReference type="GeneID" id="59337745"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17957">
        <property type="entry name" value="DEADc_DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044764">
        <property type="entry name" value="DDX52/Rok1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF15">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="233"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="495"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="143"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="665"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="672"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="700"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="730"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6226484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="739" mass="80782" checksum="068C754172105E7C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNK1</accession>
    <name>A0A8H6CNK1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155138.1">
        <property type="nucleotide sequence ID" value="XM_037299137.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336667"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05274">
        <property type="entry name" value="KR_FAS_SDR_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF37">
        <property type="entry name" value="SI:DKEY-61P9.11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="6">
        <location>
            <begin position="6"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="7">
        <location>
            <begin position="835"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="5">
        <location>
            <begin position="835"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="5">
        <location>
            <begin position="982"/>
            <end position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2110" mass="230370" checksum="A1962FE269B137CE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR60</accession>
    <name>A0A8H6CR60_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156098.1">
        <property type="nucleotide sequence ID" value="XM_037298762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR60"/>
    <dbReference type="GeneID" id="59336291"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034937971" evidence="1">
        <location>
            <begin position="19"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="179" mass="19836" checksum="387B91ABE27F35AC" modified="2022-01-19" version="1">MLFSLTPLTLGFVVHVLGQGCPIPNTFDYHNFTTQTIRSFQGVYGSNFNETITFCDLEIKSFELNDWRIIPVLAQSADSGQSQYNEKGAMLTGGTYLAPNGSVVWKGFGFSANGPYDCAFALNGNGSEADGYYTFLDQFNASTPAGETGPWLLYYEREPTREECSLVYRDYKCGAAWTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRB1</accession>
    <name>A0A8H6CRB1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007877</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3YL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156081.1">
        <property type="nucleotide sequence ID" value="XM_037298745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRB1"/>
    <dbReference type="GeneID" id="59336274"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11525">
        <property type="entry name" value="SYLF_SH3YL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000100">
        <property type="entry name" value="SH3 domain-containing YSC84-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033643">
        <property type="entry name" value="SYLF_SH3YL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF2">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING YSC84-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="332"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="280"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6228147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="409" mass="43364" checksum="BC307AC9BC81B2F8" modified="2022-01-19" version="1">MPFGINNPFPTSVASECKKAGKVLASFIDPRQSFGPDKVIPPQILANAKGLAIITVLKAGFLGSGRFGSGIVIARLSDGTWSAPSAIATGGGGFGGQIGFELTDFVFILNDAAAVRTFSQKGSLTLGGNVSIAAGPIGRNAEAAGAASLKGVAGVFSYSKTKGLFAGVSLEGSVLIERRDANEKLYNSRITASQLLEGGVRPPPAAEPLMRVLNSRVFAGVSPSSSDAMYNDIPIYDDSHDNVVWQGGSGRGYNEGVRTNRTGSMANDYEYNEAPKRASTWNDDVYDRPPNSGGRSRTNTVGEDYSYSDSPKPGRPTAPKPKFTPKPMAGAMGKDQAIALFTFDADQAGDLAFKKGEIITITKRTENASDWWTGTIGNRTGIFPRWVTCSILSHEYTKLTFDSNYVEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRT0</accession>
    <name>A0A8H6CRT0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156095.1">
        <property type="nucleotide sequence ID" value="XM_037298759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRT0"/>
    <dbReference type="GeneID" id="59336288"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033990704" evidence="2">
        <location>
            <begin position="22"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="541"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="673" mass="74538" checksum="6495C589EC82925F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRT8</accession>
    <name>A0A8H6CRT8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156322.1">
        <property type="nucleotide sequence ID" value="XM_037298986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRT8"/>
    <dbReference type="GeneID" id="59336515"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017125">
        <property type="term" value="F:deoxycytidyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="145"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="298" mass="32062" checksum="79886A7B4F05CFB0" modified="2022-01-19" version="1">MPSPHPHIPPAPEPLRKTFDPWNSSSTGHQRADNRLAGSTSWRASRTLKLGHQFRAGEGGGQRVFDTVGAGSKDFGRDGRTENGGWEKGAPGLRPKGWRDVGLMVSQGSQAELGTQEREKRKRKREDPESLLDEPEEPTTPEKEKERGIFTGLCIYINGSTAPAIGDHKLKHLLAEHGAHISIALGRRSVTHVIVGKPNDGRGGGAGGGLSGSKIQKEIQRVRGKGVKFVAVEWVTESVKAGKRLAEARFQGVSIAPRGVGSVYGLFKKKEEEDAENADTVNEGTKTEVGDEGKEKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS18</accession>
    <name>A0A8H6CS18_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WIBG Mago-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156245.1">
        <property type="nucleotide sequence ID" value="XM_037298909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS18"/>
    <dbReference type="GeneID" id="59336438"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903259">
        <property type="term" value="P:exon-exon junction complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039333">
        <property type="entry name" value="PYM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015362">
        <property type="entry name" value="WIBG_mago-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036348">
        <property type="entry name" value="WIBG_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959:SF0">
        <property type="entry name" value="PARTNER OF Y14 AND MAGO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959">
        <property type="entry name" value="PYM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09282">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01273">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101931">
        <property type="entry name" value="Pym (Within the bgcn gene intron protein, WIBG), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WIBG Mago-binding" evidence="2">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="234" mass="26112" checksum="87BCBCC63F3C39FD" modified="2022-01-19" version="1">MPTSTSPFPSQFPIPRSGILTTPTGDRHIPPSTRADGSLRKEIKIRPGYRPPEDVEVYKNRTAEAWKTRGSGGVPGAEGLKDLEDGKSSTGKNAKRREARKKAKVAEGEEENESLVNAKAKVECYRQSSKDSNPDKDSNPEWKDNGRAEGPPPSQEPEPEPEPEPEVEPEAEKEKQARNLKKKLRQARQLREKKDKGESLLPEQFEKVIKIQELIRQLDSLGFDSDGAKKAHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS39</accession>
    <name>A0A8H6CS39_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_006981</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156512.1">
        <property type="nucleotide sequence ID" value="XM_037297877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS39"/>
    <dbReference type="GeneID" id="59335381"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="9">
        <location>
            <begin position="18"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6228870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="352" mass="37345" checksum="E712AF8124F95B38" modified="2022-01-19" version="1">MSHDIPEKQWAQVIEKTGGPVDYKQIPVQKPGPDEVLVNIKYSGVCHTDLHAVNGDWPLPTKLPLVGGHEGAGVIVAKGELVQDVEVGDHAGVKWLNGSCLACDFCQTGDEPLCAKALLSGYTVDGTFQQYAIAKAAHVARIPKDVPLDAIAPILCAGITVYKGLKESNARPGQTVAIVGAGGGLGSLACQYAKAMGLQVIGIDTGDEKEAMVKKLGAHSFVDFAKSSNVVKDVQAATEDGLGPHAVILVAVNEKPFQQATEYLRPRGTVIAIGLPAGAYLRAPVFETVIGVKTIKGSYVGNRKDTSEAIDFFRRGLINAPFKVIGMSELQKVYDMMHEGKIAGRYVVDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7B3</accession>
    <name>A0A8H6F7B3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000029">
        <property type="protein sequence ID" value="KAF6217413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146848.1">
        <property type="nucleotide sequence ID" value="XM_037297825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7B3"/>
    <dbReference type="GeneID" id="59335329"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="286" mass="31415" checksum="F3BD17F0E9A05328" modified="2022-01-19" version="1">MSLLLPSNFSLATNASAYHGGLSVFPAPIAPDVVRCSSFLSEERPLDGDKCRSTWDLLPRGSNQSPWFTNPESSPAGKFQSQLPIKRGDEKCSIKVSASGKPTEEVNFAVYISGNTIRDMVGRIIEECVGAREESQQTPWGGVGTRSIRQTLDWIVQPGTGFPESLDIPTRTTFITAMVYSNERLTDYEPGAQSKVISRVLLDWIDAAAGRLPHGSPFWGVLANRFFYVWKANEDQESGQGGKGEFPHWWDDVQVPPNLERGRANGTKGAGGAVRSTLRDLRKTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8B4</accession>
    <name>A0A8H6F8B4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thymidylate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148427.1">
        <property type="nucleotide sequence ID" value="XM_037296445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8B4"/>
    <dbReference type="GeneID" id="59333942"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="42430" checksum="CE3BEC6D0A868F64" modified="2022-01-19" version="1">MTTLTTMASPARLPFASLGGARLRNMTNIKNKQNALVPTAASALKRHAPAEFDDVDSENIDPFTFSSPSKKGRAFDFDLTKSNDTPLFAPAPVQPAQYVERAQAVGQKRKAEDTVTPAAGIKRRAEPSSAPAPAGRSPKNKRIGILSRRRMTTSPFTRVNPPSSSTTESTNGLPFSIDAALAGTVPKYKSKSTHGKGWQFDIHEDTPEDEMANLMEHSTCTLEISDDEGSSSKGDKDNKENIPPIDGPAAVASATQVTATRRDMMTDETREPLGDLEAKDFYAEGCDASSCIIIAAENSSERVLDKLSTTINTQEPSSSLRSDAGAATEAQSGWEDVIARFAAKTSTSAADADFGIEKEGAKEATEIQIWESESAKGDDDADAQGPETDGVKPQSLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8D4</accession>
    <name>A0A8H6F8D4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005887</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147973.1">
        <property type="nucleotide sequence ID" value="XM_037296792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8D4"/>
    <dbReference type="GeneID" id="59334292"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="70"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="707"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="753" mass="84604" checksum="E94D519081B5565F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8R8</accession>
    <name>A0A8H6F8R8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin thioesterase OTU</fullName>
            <ecNumber evidence="11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_005191</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148800.1">
        <property type="nucleotide sequence ID" value="XM_037296102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8R8"/>
    <dbReference type="GeneID" id="59333597"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22745">
        <property type="entry name" value="OTU_OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000016">
        <property type="entry name" value="Putative ubiquitin thioesterase otu1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000096">
        <property type="entry name" value="Ubiquitin thioesterase OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048857">
        <property type="entry name" value="OTU1_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057766">
        <property type="entry name" value="Znf-C2H2_OTU1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312">
        <property type="entry name" value="HIV-INDUCED PROTEIN-7-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312:SF0">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21403">
        <property type="entry name" value="OTU1_UBXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24560">
        <property type="entry name" value="zf-C2H2_OTU1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OTU" evidence="13">
        <location>
            <begin position="159"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="80"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6219365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="362" mass="39363" checksum="46AB438FCACC7654" modified="2022-01-19" version="1">MRVRIRGPAGQAAISLSETATVEDLRTQITERTSISKFDLKHGYPPTPLSLGEFAKTAKLSDIGINLDGEQLIVSETTQAPSVNGLPKPLDPSPSQVFADKALNPSSADTPSSFSFADVGTAPPSSTQKTNKPLSLTRKPTSASLDAPELPLPSHASTLVLRIMPDDNSCLFRAFNTAFFGAMDNMTELRSIIAQSIQVNPETYSAVVLDQQPDDYCRWIQTEDAWGGAIELDILSKHFDVEISSIDVQTLRTDRFNEGRPERCILVYSGIHYDAIALSPSDAPHKTAYAPPELDTKIFDAQDLVVLEGALELCRVLQERHYYTDTAAFTVRCNICGKVSVGEKGATEHASETGHYDFGEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8S5</accession>
    <name>A0A8H6F8S5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148636.1">
        <property type="nucleotide sequence ID" value="XM_037295938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8S5"/>
    <dbReference type="GeneID" id="59333432"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="117" mass="13337" checksum="E9415CB3FA47EB77" modified="2022-01-19" version="1">MTHQTQQKRSPRPQQLKRSEHMNYTSFKDLGLTSPEQDEFENHGNQGTGLHQEMAEQAGKAEGSGGRETEIVKGECTRQRSGSMRLMEWFMRLRNQGFPYIYQNALLTIVGHLGGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F933</accession>
    <name>A0A8H6F933_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003657</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149267.1">
        <property type="nucleotide sequence ID" value="XM_037294579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F933"/>
    <dbReference type="GeneID" id="59332068"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF0">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="6">
        <location>
            <begin position="159"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="423" mass="46340" checksum="CE91806C8769EBC3" modified="2022-01-19" version="1">MGKKRSHDSGGGFLESSQKKQKHTASSSDKSLIPKPASSHVGQSLSQVSESQKNTRKLVSEPLSDSDREAHDHPQEASKSTQAQVQDPRLSGYALHVKQSNANGDSISLGKALNFPVFQQSSSVSTQHRPSSTSPKESVKFPPYNGRPSTLPPLPPILDKALQSVAFTHPGSLSCDTASKVNISYDRLEFLGDAYIELMATRVIFPRFPRLAAGRLSQQREMLVKNETLAEYALAYGFDEKAKLPSTYNTPGKDSRKLWLKTLGDIFEAFVAAVIISDPEQGFQVAEAWLAALWELKLGSQKREDTEIVDPKAKMQLAAKIMGKGIKIHYRDEAPPVEIRQEGKLIFQVGVYLTGWGWEDQHLGSGKGLNKQEAGQKAAADAMVNPLTAQVASVKRDFDAQVALERSFQDEADKEKQGGVGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9K0</accession>
    <name>A0A8H6F9K0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003563</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149173.1">
        <property type="nucleotide sequence ID" value="XM_037294485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9K0"/>
    <dbReference type="GeneID" id="59331974"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001425">
        <property type="entry name" value="Dynamin GTPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF215">
        <property type="entry name" value="DYNAMIN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="5">
        <location>
            <begin position="30"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="4">
        <location>
            <begin position="634"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="418"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="721" mass="81383" checksum="5947F587A28CEB69" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9K4</accession>
    <name>A0A8H6F9K4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chitinase</fullName>
            <ecNumber evidence="3">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_004148</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 18 family. Chitinase class V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149114.1">
        <property type="nucleotide sequence ID" value="XM_037295067.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332557"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029476">
        <property type="entry name" value="DNase_NucA_NucB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF402">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14040">
        <property type="entry name" value="DNase_NucA_NucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="11">
        <location>
            <begin position="77"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="944"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="952"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1691" mass="186936" checksum="45063A6C67DE4994" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9V7</accession>
    <name>A0A8H6F9V7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149488.1">
        <property type="nucleotide sequence ID" value="XM_037294800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9V7"/>
    <dbReference type="GeneID" id="59332289"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="80" mass="8307" checksum="8612E8BA2EE8A8B5" modified="2022-01-19" version="1">MAAKDSTIPKSLAVTRLGIGEAIRRNVNVAVNSPSGDKERVQRHEQIANKGVAEIDQGRAVRGSGEAYPGPGLGSGKVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAK8</accession>
    <name>A0A8H6FAK8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Maintenance of ploidy protein mob1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150780.1">
        <property type="nucleotide sequence ID" value="XM_037293127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAK8"/>
    <dbReference type="GeneID" id="59330614"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="30952" checksum="951CF08547BACF89" modified="2022-01-19" version="1">MFVSPPFPDISPKPLPAVSTPVQIAPFRTSLAIHATLYAAATKQPKQAHENEDLSLSYDVARNARTRGQFKPKHANKGTSSYQLRQFAEATLGSGSLRKAVKLPEGEDLSEWLAVNIVDFYNQINLLYGSITEFCSPQSCPEMKATDEFEYLWQDSENFKRPTKMPAPEYIEHLMAWVQSNIDNESMFPSRIGVPFPKSFPVLIRQIFKRLYRVYAHIYCHHYPVVVQLGLEPHLNTSFKHYVLFIDEHSLASGKDFWGPLGDLVDSMLRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBF7</accession>
    <name>A0A8H6FBF7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="12">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 12">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_001301</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the snRNP core protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151650.1">
        <property type="nucleotide sequence ID" value="XM_037292231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBF7"/>
    <dbReference type="GeneID" id="59329717"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01720">
        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000018">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="35"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6222215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="117" mass="13440" checksum="0B9CB6D621DA68FA" modified="2022-01-19" version="1">MADAKIQDLLNKPRMELTEYEISVLEEHEFTSGPLSILQTAVRSHTQVLISCRNNRKLLARVKAFDRHCNMVLENVKEMWTETPRISGGKKGRPVNKDRFISKMFLRGDSVILVLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBG1</accession>
    <name>A0A8H6FBG1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151665.1">
        <property type="nucleotide sequence ID" value="XM_037292246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBG1"/>
    <dbReference type="GeneID" id="59329732"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="223" mass="25099" checksum="A90D5F1DAAF9B43D" modified="2022-01-19" version="1">MFGQRRLLAWIRKTDPRRLHQQLQNSDQLAASRHQQLRQQSPRVCTSLAEGNARLGSNTNTDKVHRRWVPFTATRSLKLIEVIGTNFVCTITVVDMDIVPQRYVRPARYRITDVATFYDIRNALSEIWMHCLYGPPERQVGYAVTGTSPGAHNSDLRASDYCWLTLWIGLGNSLGVFVYGQHASIAFGMPRTPISMPLPAFAIGDGEQLDPSGRVLKDYLPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBK1</accession>
    <name>A0A8H6FBK1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001747</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151214.1">
        <property type="nucleotide sequence ID" value="XM_037292675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBK1"/>
    <dbReference type="GeneID" id="59330161"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12710:FF:000004">
        <property type="entry name" value="Protein arginine methyltransferase NDUFAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF7">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="240"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="504" mass="54119" checksum="640A79057B47F02E" modified="2022-01-19" version="1">MIIRPGVKIIASSRPLARGCSGLKVDKRIRWASSPARQWSTPLAKTIAEAIATTGPISIAAYMRQCLTSPDGGYYTSQTQGKDQFGQKGDFITSPEISQIFGELLGIWLVTEWMSQGRVSHGVEVIEVGPGRGTLMDDMLRTLSNFKPLAASIESVYLVEASPALRDTQRQLLCGDVPLEEIDIGFRSTSKYSRVPVTWCEDIRFVPNDSSKTPFIFAHEFFDALPIHAFQSVAPNSTETSQFLTTPTGPLPLTKPASQSKTPQWRELVVTPTPSASNITTTTSPSTPKSQPDFQLSLAKASTPPSLLFPTLSQRYKALLPTPGSVVEISPESHSYAADFARRIGGSSAKASSNCTSSTMVKNRPSGAALILDYGPADTIPTNTLRGIRSHQHVSPLTDPGLVDISADVDFMALAETALAASPAVEVHGPVEQGGWLQSMGIRERADIICKGLGEDEGGKKRVRGAIQRLVERGGGAMGKLYKVLAIVPERDGRRPVGFGGGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBL6</accession>
    <name>A0A8H6FBL6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001513</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151862.1">
        <property type="nucleotide sequence ID" value="XM_037292443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBL6"/>
    <dbReference type="GeneID" id="59329929"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF194">
        <property type="entry name" value="INVERSIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="233"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="306"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="340"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="396" mass="43330" checksum="9018C3DC0E066888" modified="2022-01-19" version="1">MSDINPSVEIYRSCLPVLSIVFLGAPHKGLHTTALETLVKSRPTEDMIQGLKAESPTLTDLNDKVRYVAREINILTCYELYPTKTAVQLSWPREESISCHVDHSQIAKLERGESSVYPSVSCAIESAIEEQTIFGLSRFQVSDAIASEDSGTIHYNDLKPGPSRSSGHAGNITIDKGRDYQPPLRLNLQIFYRQNSSAPPSGRKAQELLARGCSLHSVDSRGSGIPPEAYPKKGKDPYLLAAYLRQGKVLKLLLEHGADSSRAESDGVTALHAFIFGVFKTETPPTESIVALLLQHRTPLEQVYWMGWTPLMYSVDLGFLLVLTKTLLDHGANVNIFNQSGAIILHYAARGENPDIVALLLHKGADVDRTSPDGQTALHWACSGSASSSAQIVRML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBM0</accession>
    <name>A0A8H6FBM0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001866</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151333.1">
        <property type="nucleotide sequence ID" value="XM_037292794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBM0"/>
    <dbReference type="GeneID" id="59330280"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42044:SF2">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42044">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="68647" checksum="0933F228323CFC91" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBU3</accession>
    <name>A0A8H6FBU3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151951.1">
        <property type="nucleotide sequence ID" value="XM_037291589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBU3"/>
    <dbReference type="GeneID" id="59329070"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="1">
        <location>
            <begin position="167"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="419" mass="48089" checksum="88FEA12B2B4C0219" modified="2022-01-19" version="1">MAPTRAHESLRPPLIHQFKFDDFNIYRLMAENAMHDGDYRAQLERSAYGHDKPIELSKLNGEIENGMAALGQKHINDEEEAWIQFCRQVFKAGERRGTAVSRQTSAMNGQPSMAGLPETNGRDWGNMLWARDREWMFTGTASSSARRALTMLAANQRTEADSRPFQLMKLPTELREQVYRELLIGEGRRIVVSADSVSSMWSRSCKMEGPYVYGKEITTALLRTNRRIHDEALPVLYQSHAFDFGIDVHYVAPFFDQISTAARQNVGCIHMELIRYGIGRPLRSVPERDVNDNCLDWSDACAYIAGHLRVKEFSFNINFTIPQDFQRFRWVKDLIQVRGVSAVFHHNSANKHGLKSENAWIQLANQMSREDWGADEYRWETLLEYLNSEMLHKLSNEMTASVEPQWRGPGLGVSFSLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCH8</accession>
    <name>A0A8H6FCH8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000924</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152219.1">
        <property type="nucleotide sequence ID" value="XM_037291857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCH8"/>
    <dbReference type="GeneID" id="59329342"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05288">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF19">
        <property type="entry name" value="ENOYL REDUCTASE (ER) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="21"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="318" mass="34564" checksum="74B48B7BBAE5111E" modified="2022-01-19" version="1">MSSPTQWVLTNKPTDLPELSGPNQTFTLETASLPSLNDDQVLVRSLYISNDPAQLPQDGIMRARSIAQVVESKSSNYSRGDKVLATTGWAEYSVQDAVECQACEDIPGLSATHYLGAFGLTGLTAYYGTKIIARVGREDTVVVSGAAGATGSMVVQIAKKMLGCKKVVDIAGSDDKAKWVESLGADVCLNYKKFCFKQDLVKATDGFVEVYFDNVGGEILDLMLMRMKQGGRIAACGAITDYNRNEKQTTLKNWFEDFWAEGKGPQAVKEMVTAAKEGKIKVGEENETVVDNKFEDVPKTWMSLLEGANTGKLVTKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD56</accession>
    <name>A0A8H6FD56_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000559</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152933.1">
        <property type="nucleotide sequence ID" value="XM_037291498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD56"/>
    <dbReference type="GeneID" id="59328978"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20524">
        <property type="entry name" value="CYCLIN_CCNH_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20525">
        <property type="entry name" value="CYCLIN_CCNH_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000095">
        <property type="entry name" value="Cyclin Ccl1, putative (AFU_orthologue AFUA_5G07030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031658">
        <property type="entry name" value="Cyclin_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16899">
        <property type="entry name" value="Cyclin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="87"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="395" mass="43726" checksum="217DA8D14AF6A655" modified="2022-01-19" version="1">MTEDDIYRTSTQYRLWSFTRESLASLRSTTNASAADGVRAAIRSQHVGQAGSDPAREGSNDKPSDPAKEVDCLTVEEEQKLVGFYCVKAMQFADFCEFPTNVKATAVQYLKRFYLSNSPMTYHPKEMMAAALFLSTKTENHYTSLRSFASKLPKTTPESVVAPEFLLTQGLRFTFDVRHPHRGLEGGIMELLGLAQGKGQAGPGIKQSSEQLQKEMVDIEPAKESSKKARSADEVVRRIQVAHGKAKEVGKSSALLTDAYFLYTPSQIWLSSLFLADEPLARFYINTKIPTTSTLTPKLISTLQSCAELLRSSASANPGEAEIKALTRIDKKLFKCRNPDKVDLVGINKAQKRDSKGAGENGLDERVIKKRKLEREKSEKEAEGIFGPTLAKETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD87</accession>
    <name>A0A8H6FD87_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peroxisomal-coenzyme A synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010649</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153135.1">
        <property type="nucleotide sequence ID" value="XM_037301501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD87"/>
    <dbReference type="GeneID" id="59339039"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05926">
        <property type="entry name" value="FACL_fum10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045310">
        <property type="entry name" value="Pcs60-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF5">
        <property type="entry name" value="MEDIUM-CHAIN ACYL-COA LIGASE ACSF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="14"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="419"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="56200" checksum="6128C2BA15FBAD96" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDF3</accession>
    <name>A0A8H6FDF3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153330.1">
        <property type="nucleotide sequence ID" value="XM_037301696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDF3"/>
    <dbReference type="GeneID" id="59339235"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07040">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF27">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="595"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="686" mass="75644" checksum="7459E213E304C68D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDN6</accession>
    <name>A0A8H6FDN6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010602</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153088.1">
        <property type="nucleotide sequence ID" value="XM_037301454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDN6"/>
    <dbReference type="GeneID" id="59338992"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008318">
        <property type="term" value="F:protein prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF3">
        <property type="entry name" value="PROTEIN PRENYLTRANSFERASE ALPHA SUBUNIT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="325" mass="37092" checksum="5FE07D9A06B1B993" modified="2022-01-19" version="1">MPSPPSPPHDDVYAQLIAALHPSPPVVLEVEILPSSFPPQLLHESAAVGVPKSLLVPLYLKARKTFFDHVSSRDSDSYHAALQATAVILLFDPNHLTAANFRKRHIQRVSGKRSENDYNDRDLGNLAEAVRHELNFLEILVTSPLSKHAKSSTLWAQRLWTGQNFFPLDLQEHAADEANQVMITGNGIKSFWDEELVIVMKAGERHPSNYYAWQYARQLFTSIRFERPGYKDRRHGVRVLLYDSIGLVHGWCLMHPRDISGWAFLVFLLEQLRDEGCDEGDKTKVSEDEILISASETKEFAKKYEWKGESIEWFLKAMKTLDIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDX6</accession>
    <name>A0A8H6FDX6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_010891</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the second and fifth step in the 'de novo' purine biosynthesis pathway; contains phosphoribosylamine--glycine ligase (GARS) and phosphoribosylformylglycinamidine cyclo-ligase (AIRS) activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">In the C-terminal section; belongs to the AIR synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the GARS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153374.1">
        <property type="nucleotide sequence ID" value="XM_037301740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDX6"/>
    <dbReference type="GeneID" id="59339281"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000006">
        <property type="entry name" value="Phosphoribosylamine--glycine ligase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000007">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="119"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6224314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="805" mass="85785" checksum="AD9696045DAD49D9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE40</accession>
    <name>A0A8H6FE40_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pinin/SDK/MemA protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153857.1">
        <property type="nucleotide sequence ID" value="XM_037301040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE40"/>
    <dbReference type="GeneID" id="59338577"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006786">
        <property type="entry name" value="Pinin_SDK_MemA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04696">
        <property type="entry name" value="Pinin_SDK_memA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pinin/SDK/MemA protein" evidence="2">
        <location>
            <begin position="71"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="148" mass="16676" checksum="C1D6B76992D66978" modified="2022-01-19" version="1">MAEAPVAVASAVILPESEQTPSPPAALKRRQSSFSDDHSKRPRLGHYDTNGSRPDTMTNPQSERRRSGQMEERKRGQRLFGALLGTLSQSSSSTAQKRRTDIEKKQQAKLKLQAEEQDEKKKQKLEALLVVRRGEQKKFDKQSVSFAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEA5</accession>
    <name>A0A8H6FEA5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154067.1">
        <property type="nucleotide sequence ID" value="XM_037301250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEA5"/>
    <dbReference type="GeneID" id="59338788"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034757937" evidence="2">
        <location>
            <begin position="23"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="539" mass="58493" checksum="CDCBFDEBA9A1E86F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEH8</accession>
    <name>A0A8H6FEH8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153978.1">
        <property type="nucleotide sequence ID" value="XM_037301161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEH8"/>
    <dbReference type="GeneID" id="59338698"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="372" mass="40657" checksum="259709E3B721E69B" modified="2022-01-19" version="1">MEEDRKRDDPMDLDKPEDAPPAYTALGSPSGLTDWTEKEDKVKTDHDEAEEEEEEEKTKEEKGARRKTKGKAKASPPPPDDNDDNNNNKDQPPRVARPTPNPSPPRTALNSDTHTDMDTYRALEAHKALEPAHVRGTHFADADATMNDTLSSAPPSPNTENTAPGPRNGPTGSFDSAELRGKLETLRQRQQQRGDGGRGQGEGWGFAWQETMTREKVEKEGWGGDDDDDAFPSPSAQAVREEAGWEVYDEAAWETAAGRAAKQERRVVALKVPRASFSVRGESGTAGRRGELVRAVRGYEAILSGTFLTYQRDDVMRVVHRDGDGKVFTLLPLKAGAVAARAHTQDFVPASSAAGSEASDEGSWERLERKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF89</accession>
    <name>A0A8H6FF89_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154388.1">
        <property type="nucleotide sequence ID" value="XM_037300540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF89"/>
    <dbReference type="GeneID" id="59338074"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39609">
        <property type="entry name" value="RFEG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39609:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR RFEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="379" mass="42962" checksum="A23065290FEF5A26" modified="2022-01-19" version="1">MSRRDRDTRDDRDRRRGDRGGREEPVPAPQRERPRPSKQRQDALNEFFVSGEGIHREVLQREICKYLGPEAYSRPSTYNGNNGYVITAVRPFTSKMLDDLIELSNLYAAENREMRKRGQDISYGESETSRRQEMRTGAEEPPYPERFPSQPTYAVPPPQPAYSTGAGSAAYPGSVYPSGSSYQPGPSFPATSGYLTPGHLASIRPSTNDSNYTYGDEYTNPGPSYRQATTYPGTGGYRDPREEPRAYSRADPRDTRMDPRDPRLDPRDPRLDPRDLRMDPRDPRMDPRDPRLDTRPDPRTIPSYPSYGVNSPGDVSMHGAVDDPRAYDYVSTIPPLQSGRGGSFTPSGVVPRGYDPRDSPQMRDGYRTEPIREERRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFC0</accession>
    <name>A0A8H6FFC0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009785</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the VPS35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154492.1">
        <property type="nucleotide sequence ID" value="XM_037300644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFC0"/>
    <dbReference type="GeneID" id="59338180"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.660:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="332"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="884" mass="99856" checksum="86033BD3218CC61A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFF6</accession>
    <name>A0A8H6FFF6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CorA-like transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009236</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154923.1">
        <property type="nucleotide sequence ID" value="XM_037300097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFF6"/>
    <dbReference type="GeneID" id="59337631"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058257">
        <property type="entry name" value="CorA-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26616">
        <property type="entry name" value="CorA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="531"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="CorA-like transporter" evidence="2">
        <location>
            <begin position="6"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="CorA-like transporter" evidence="2">
        <location>
            <begin position="89"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="573" mass="65643" checksum="C039D497F42CCEBA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGD9</accession>
    <name>A0A8H6FGD9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fucose-specific lectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155217.1">
        <property type="nucleotide sequence ID" value="XM_037299216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGD9"/>
    <dbReference type="GeneID" id="59336746"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.70">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="31999" checksum="64E1509D19FE271E" modified="2022-01-19" version="1">MALTNLGYGRTVTSCEGMEVDLSAARPHELPDSLGIEATPGEFYPTCTPQPVKAPEKELAHDRIPPGSPSSLKPKRFLPLLVAAIIVAVIVLALAIPLALRLKKPFNPSSPVNSKSTARVQDELPTSGAFNGTGLAFIALDLTDVPDVSLFYQDYDARIRRLHSIELSPCVGGFPIVASNARNTTPLTTLTYPASNGELNLGQDGNSPGIRLYYGAPDNKVHELALFPNISSQYFSHSVIPGTNGNAGIASGWSDDSGLGNLYVFDKNNEFQIWSNNFNTTHDATQNASYDNWIEGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGN0</accession>
    <name>A0A8H6FGN0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NWD NACHT-NTPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155486.1">
        <property type="nucleotide sequence ID" value="XM_037299485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGN0"/>
    <dbReference type="GeneID" id="59337015"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20324" checksum="964D24F37A4053B7" modified="2022-01-19" version="1">MRLKDVFSRKHGNIVKASSWREADSTRNKNPQDGSAASLAGGMGSLNLDGPDVGLSDVERVSQHETPAKASKFCKKSYWAIAAQQLQRAQPALAEALLKIQSAALLEGQNLAESIQALKDMGSQIAGLDPLHAGLPWAGFCLLMQTALSESDQYAAMVAGVEEVAKLISRYTHVEFICQKRQEVTLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI71</accession>
    <name>A0A8H6FI71_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SAM-dependent methyltransferase TRM5/TYW2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_007095</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the type 2 lipid phosphate phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156623.1">
        <property type="nucleotide sequence ID" value="XM_037297988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI71"/>
    <dbReference type="GeneID" id="59335495"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03388">
        <property type="entry name" value="PAP2_SPPase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="10">
        <location>
            <begin position="873"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="409"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="486"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="502"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6228981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1178" mass="130743" checksum="52C37A08FC39B0EE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI79</accession>
    <name>A0A8H6FI79_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAM50A/XAP5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156651.1">
        <property type="nucleotide sequence ID" value="XM_037298016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI79"/>
    <dbReference type="GeneID" id="59335523"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048337">
        <property type="entry name" value="FAM50A/XAP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007005">
        <property type="entry name" value="XAP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722:SF0">
        <property type="entry name" value="PROTEIN FAM50A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722">
        <property type="entry name" value="XAP-5 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04921">
        <property type="entry name" value="XAP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM50A/XAP5 C-terminal" evidence="2">
        <location>
            <begin position="205"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="402" mass="44312" checksum="E2794B3EDFABD61B" modified="2022-01-19" version="1">MASNPSSNDTSRVPTPNPRFTTQASTAADLLASQTVGLVHLSDFRKRRAEALEQKEREAQGTAVSFGRFAPASSSASGNSVSDVNNSIINGVSTSHGQPPKKKRKHVAKGKLSFGVDDEELDRVERIESTDLTPRDGTPDEDGPTRASPASSRNDFSTERKRFGANANLSVAPKAMTKSALLKEAQTREQLRKEFLLMQEAVKATEIIIPFVFYDGTNIPGGACKVKKGDYVWLFLDRSRKVGAELGVGGGEKANSRREWARVGVDDLMLVRGEIIIPHHYELYYFIVNKTVGPNSTPLFDYAAATVPPSDGSESPDQISDDYDPISLPSKTKQKNNGQVTPADRHLEGVNADPNFTKVVDRRWYERNKHIFPASVWEEFDPTKDYTKGVRKDAEGNAFFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIQ3</accession>
    <name>A0A8H6FIQ3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156894.1">
        <property type="nucleotide sequence ID" value="XM_037298259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIQ3"/>
    <dbReference type="GeneID" id="59335767"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="326" mass="35694" checksum="7E7C9A5E21B8578E" modified="2022-01-19" version="1">MKFTKSKKETKKSPEEEYVERISTLTESELRDLHKDLKIDHYVNSTAAVINTASGVGLLSMRSTTRRYRTERMQIEAMEARMNEMGWTAHPNRYRDAVPIALGVAAGTTAGMSFGLINVEKNAKRTTDYVKKKDLFKTKELVETKEIDAETETEEAEVAEEVEVAESSPERTQTHEKTTEPVIVSHSIARKPVPAPAPTNTIAAPSQPENLNTAVEITHDTRVVSIEPTATEPPAPSVHACPAAEPKPTKKEALFSQVSVMNKKMRSFGFKRDGEEKETTVSVAEIACDARSPAMDAEAGAVVGHGVVEKLDGPPLYTAEAEECVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKV6</accession>
    <name>A0A8H6FKV6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157632.1">
        <property type="nucleotide sequence ID" value="XM_037295631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKV6"/>
    <dbReference type="GeneID" id="59333123"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="255" mass="27951" checksum="B772435672D12658" modified="2022-01-19" version="1">MSALVDDLAMRIPPSSCLVCQFKKLRASCSNTENKTTVLVFDPSTGVYTLKKIEDIDAEKQNFIYRKGLPTSAQSIKVQLPGETKLLAEVRVDSARTRQGLKHQRSLVDGFYIMPGRPGTQMTMNPKRTREGQLAELPAPGFFNQTRHPVELPADETSPVPALPSSPTPLLLPPRTPPKNARKAPQLLAPPPREVFSEKRTSYGSSLGSASIHIAYLPSHQDDVERDGIPDPCNVSPSIPHPGLRVSILRAFIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL32</accession>
    <name>A0A8H6FL32_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157750.1">
        <property type="nucleotide sequence ID" value="XM_037295749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL32"/>
    <dbReference type="GeneID" id="59333243"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="507" mass="56399" checksum="39519FA3E4DB1F59" modified="2022-01-19" version="1">MHAENVWKWLDAVDQDNLDPLDQWGVPPLRCPHQDTPPPSNSVSDGGDMETYQQYNNKRNAEVASFLESIPPAGILLRNGSIRVTSQNPKVFACDVENDNHPQSLLPGFPTSFARVVENKDHSQSFIPGFATSFEATGQQTDASPARQLQVSGREGNSESTNPKRLPTFSERARVGGPSGALYSTPIIHVEVKDSVAINCQGAKVVGEYQQNGLRGLPSMAEKSRRNSTFGEGVLHRNNATRRLSNVRAGPKNLISLFTRHSETTGPYILGLRRASFPLQHLLVSYLHQRRKSDSRIHTLQTSRSSHNISRSSANTMSLSTSSSQQVTASQTTMSMTQKLRGVASKIFQKMKLPKSKEDRNFEELGSTEIEVARFATFIRPTAAPQIVDLQAIRQNNRPQPARIYTHDSIVPRSQADLNNPELHLQRSFETMHSPTETGTPLRRNPQQVFVFEATSDHSATAALDDIVDYADYEKLDDDDSDLDWSLLDLPENVYGRLANESRNSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY46</accession>
    <name>A0A8H6KY46_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156617.1">
        <property type="nucleotide sequence ID" value="XM_037297982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY46"/>
    <dbReference type="GeneID" id="59335488"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF10">
        <property type="entry name" value="TOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="9"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="462" mass="52575" checksum="36D031E136C1D34F" modified="2022-01-19" version="1">MRKKVLEPAISKTFKEAMLVVKALGERYIWIDSLCIIQDSPNRSDWFEESTKMHAIYANAFCNISATGAVDGRDGCFFDRSPLLVRPLRIKIAGGEDLPPGTYLCVDCDIWSGNIDNAPLTRRAWVVQERLLSRRVIHFSQRQIFWECRERQTCEIFPHGVPPQLQQSHEAMFKGIDPDIEGARARERRGRVSHPELNVYELWNSIVEAYTQASLTMEDDKFVAIMGLAQRIQELLERRYFAGLWEKYLADQLLWSPARSRGMVKPQKYIAPSWSWASFVVPITSPCLIRDTTDDNTLIQIVDLDVQTINDHPLSQVTSGSLRLRGSLVRGRIWKEISDGGRLGELTTVKYDIYDNGKLEQVDYIPNLDVSDPPGSVYVLPVKSITYETNGGLRARGLLLQLTGQCIREFRRVGTFQVRDLRVQNAHTQYVPLVDGDPLSADDIWLDGFKLDGKTLDTLTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZP6</accession>
    <name>A0A8H6KZP6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1754-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157416.1">
        <property type="nucleotide sequence ID" value="XM_037295415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZP6"/>
    <dbReference type="GeneID" id="59332906"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013865">
        <property type="entry name" value="FAM32A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13282">
        <property type="entry name" value="PROTEIN FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13282:SF6">
        <property type="entry name" value="PROTEIN FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08555">
        <property type="entry name" value="FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15288" checksum="5C89044C9DBDA2AC" modified="2022-01-19" version="1">MPADDYTAAVSGGLKLKSVASSSKVSKSHKKKRPKPSQPESSADANAEKSKDGATDHGDDVKDEHDDATVDKSQQASEEAEALSPVRAGKTEAELRHEERRRKRLDERLKREGTKTHKERVEELNRYLSNLSEHHDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZS5</accession>
    <name>A0A8H6KZS5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_004561</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157478.1">
        <property type="nucleotide sequence ID" value="XM_037295477.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332968"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="term" value="F:histone binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="ATPase_AAA_CS"/>
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        <property type="entry name" value="Bromodomain"/>
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    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR23069:SF0">
        <property type="entry name" value="TAT-BINDING HOMOLOG 7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="12">
        <location>
            <begin position="1197"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="268"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1116"/>
            <end position="1139"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1188"/>
            <end position="1219"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1356"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1480"/>
            <end position="1566"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="55"/>
            <end position="67"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="74"/>
            <end position="83"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="237"/>
            <end position="253"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="283"/>
            <end position="296"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="11">
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            <begin position="328"/>
            <end position="344"/>
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        <location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="417"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="444"/>
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            <begin position="1117"/>
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        <location>
            <begin position="1194"/>
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            <begin position="1360"/>
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        <location>
            <begin position="1426"/>
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        <location>
            <begin position="1480"/>
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        <location>
            <begin position="1551"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6230221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1677" mass="183771" checksum="BFD6F0656C8991C4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZT7</accession>
    <name>A0A8H6KZT7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HO133_004546</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 16">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Cytoplasmic vesicle</location>
            <location evidence="1 16">COPII-coated vesicle membrane</location>
            <topology evidence="1 16">Peripheral membrane protein</topology>
            <orientation evidence="1 16">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum membrane</location>
            <topology evidence="2 16">Peripheral membrane protein</topology>
            <orientation evidence="2 16">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Golgi apparatus membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
            <orientation evidence="16">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157464.1">
        <property type="nucleotide sequence ID" value="XM_037295463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZT7"/>
    <dbReference type="GeneID" id="59332954"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01478">
        <property type="entry name" value="Sec23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000006">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000008">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0813">Transport</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="18">
        <location>
            <begin position="60"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="19">
        <location>
            <begin position="127"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="21">
        <location>
            <begin position="405"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="20">
        <location>
            <begin position="523"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="17">
        <location>
            <begin position="638"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF6230207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="772" mass="85990" checksum="C19ADB82861D58CF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZU2</accession>
    <name>A0A8H6KZU2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7729 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157567.1">
        <property type="nucleotide sequence ID" value="XM_037295566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZU2"/>
    <dbReference type="GeneID" id="59333057"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056146">
        <property type="entry name" value="DUF7729"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39460:SF1">
        <property type="entry name" value="C6 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39460">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24855">
        <property type="entry name" value="DUF7729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034168962" description="DUF7729 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7729" evidence="2">
        <location>
            <begin position="85"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="350" mass="35564" checksum="873CAFA7D2D0499B" modified="2022-01-19" version="1">MFLFMAMIFSVLCMPTEAAEHPGSQSRAVKGPFVDGRILFDHEAAPQPALHRRDDSVSTDTASTTTAAEVAVTSSSSDPSTTPTSLPRAFDGGFGTNFTQPSCPTFLRSMVSNDTFISCVPFSLLLQNSMSFFDATRSLSTITAVLDASCSVFFPTCSALMSSLALTLRSPSACQADYGSENPQVRQAYAGLLAYDVSYGASCLKDPPAAADNHPNNNDNDNSAYCFANAVTNASAPTGSYVYYVPLGIGLPSGSLPTCDACLAATMAVYAAAAPNRTQPLNLDYVASATLVNDMCGPAFVNASVPAAAGGAGGGGGGGGGTRSAASRAGAAWGAWAGVLVGAAAVVAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZU7</accession>
    <name>A0A8H6KZU7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004577</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157494.1">
        <property type="nucleotide sequence ID" value="XM_037295493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZU7"/>
    <dbReference type="GeneID" id="59332984"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098771">
        <property type="term" value="P:inorganic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000013">
        <property type="entry name" value="Cation efflux family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1350:FF:000010">
        <property type="entry name" value="Cation efflux family protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF15">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="9">
        <location>
            <begin position="40"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="370"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="383"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6230237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="412" mass="44301" checksum="AF11D563AB8DF428" modified="2022-01-19" version="1">MTSTTLARPHSGHSHQHHHHDNTYLVSTNKKDAGVRITRIGLYVNFCMAIGKGLGGYFLNSQSLIADAFHSLTDLVSDFLTLATVSWSLKPPTSRFPSGYGKIESLGSLGVSSLLLAGGIGLGWHSCELLYSQFFLDAASSMAVHGHSHGGGVFGHSHSHNPADMGIPNVHAAWIAGGSIIVKEYLYRATMKIAKERKSSVLASNAVHHRIDSLTSIVALVAIVGSHVLDNVSWLDPIGGLVVSMMVIRAGWGNTGSALLELADVGVAEEIKVSVRTTASKALGEDRFGSGRVMGNQIEVRDVQGVKGGQNYLMNVELAVPGEWTVQETRLVEDAVRERVGSKVRGVRRIRVRFVARSDEAPDFADEFIGADVSSSSPEPDDLDHTDSHSHNHTIDHTHDKALSNGDAKKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L023</accession>
    <name>A0A8H6L023_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7053 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157778.1">
        <property type="nucleotide sequence ID" value="XM_037295777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L023"/>
    <dbReference type="GeneID" id="59333271"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055481">
        <property type="entry name" value="DUF7053"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38117">
        <property type="entry name" value="NACHT AND WD40 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38117:SF2">
        <property type="entry name" value="NACHT AND WD40 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23155">
        <property type="entry name" value="DUF7053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7053" evidence="2">
        <location>
            <begin position="2"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="307" mass="33600" checksum="64CEAA67F3E1261E" modified="2022-01-19" version="1">MSKKSHFTTVTALPAGITRETVLETLHNHVAMIDLNPLVTERHPIKPPSEATPEEYHCQWYSVTDKVNYLPGGLYSGSVTFNACFHDLPDGLQSHIYAPMGLNMKGRWTLGGSLPGEPRQAVEIGLGVPKTGLWLREDVEMKVNVVMGGFVKKTTKKAHSTLVRRLVEKAHIIDAETHNTGLAEQTFQDSYPPGYHSGGTPAAGMPYQDRRSMRSGSGTCSDDQSVVSGGSHDRYSYASNPQIANPSNSSYSDYSQRPDAKTSTSYPGVAPLFSQQSHPASFAVELPSQRESSPMELDSTQRYPSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6X4</accession>
    <name>A0A8H6C6X4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147457.1">
        <property type="nucleotide sequence ID" value="XM_037297332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6X4"/>
    <dbReference type="GeneID" id="59334835"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="881" mass="98801" checksum="CABEDDBBE5A014D3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C714</accession>
    <name>A0A8H6C714_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147296.1">
        <property type="nucleotide sequence ID" value="XM_037297171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C714"/>
    <dbReference type="GeneID" id="59334674"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="28882" checksum="ABB72DB8D5833F01" modified="2022-01-19" version="1">MNATKEGNELHDLEDANLIGDLDRSHTRYSTWFARQRPAPAQELSAKTTYTLLPIALLPMINQPPPNIEPSCLPDSRRLVVHDESLIRWLLAHGADPNASSRRMGFYAALAPLEVINLLFERGGSTANGRLLNRASDPILQCSPDRGVPINDTLLEHRPGLAHRANAGAAATPFHKCGCGRKCRVGEVFSRTVAPLARIKLPFDTAVGCEQTVTAKILAQGPPLPAPRSGIRAARGWRYAFCTSGGKRSGGSPLGFRVGRLWEIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C732</accession>
    <name>A0A8H6C732_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147326.1">
        <property type="nucleotide sequence ID" value="XM_037297201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C732"/>
    <dbReference type="GeneID" id="59334704"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="178" mass="19181" checksum="747FA23FD2474C31" modified="2022-01-19" version="1">MEAEERKRKHSESSGDINNINFNISLGKDFQGLLGGVNPSDSQSPAKKAKTGPSKPTKRKTEEFGSSKPSKKATELGFEEPLSQARKIPAKVYDPFSLAEGDLPYPSNTNIGAAEEISLAGQKPILARAKRKLKVEGTTQTGKEVGVGKELNARKEARVKKELGAKPLSKIKHGLDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7P7</accession>
    <name>A0A8H6C7P7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147862.1">
        <property type="nucleotide sequence ID" value="XM_037296681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7P7"/>
    <dbReference type="GeneID" id="59334181"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47381">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47381:SF3">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="39286" checksum="3D6C261C30CD41F1" modified="2022-01-19" version="1">MATPNPMANTYPIPPLPVSKKTVCIAGIITTVYGLDELDAKVQDVACLWLLHPRLQTQECMAPVAASTVNAWREEKQKPKKGKALGLICVSFDQRNHGSREVDALSNEAWRSGNKTHAQDMFASYHGTAIDTFHLMNYITSYVFPTSQHVIVNHLVLGVSLGGHAGWHCITHDPRITTAVVIIGCPDYVRLMSDRARLSKLATWTDSDPPGSSFLGSTDFPKGLVEAVERYDPAGLLLGEIVSRDDDVYDRVPTASEQQRLVPLMKRSLQGKRILNMAGGDDKLVPYKCGEPFLRWLKKATASDGWFNEGGVVFEDIVFDGVSHDMSPSMVREAHRFIIEALEQTPTEIAGRASKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C881</accession>
    <name>A0A8H6C881_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006016</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148100.1">
        <property type="nucleotide sequence ID" value="XM_037296919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C881"/>
    <dbReference type="GeneID" id="59334421"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061685">
        <property type="term" value="F:diphthine methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052415">
        <property type="entry name" value="Diphthine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042:SF1">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="406" mass="44896" checksum="1552F986E534371B" modified="2022-01-19" version="1">MDGSDTADDAPPISSDYSIILDLPPSCVEFSPSHPDCFVVGTYYLKPESGASHTTSIQQEDDQIGPPAQDRRGSLMLFNLTDERLALRHNVHLDYAILDLHFTPFRKGIFAIATSLGTVCLCTTNIQGTGPIEIVNSYEIFPISSLVLSLAWNRELSHSDTIAASSSDGRIAIFDSKYQPPMTYTKTEAHSLEAWTLAWTSKGAEDDLRPELYSGGDDSVLCRHGLSVRPVSGSLDEGNIPSHRYDFQAPFRDTKTHMAGVTAILPLQPVFHGEQILLTGSYDEYVRVLMPIDGYSRPKILAEERLGGGVWRLKILDFEAPDRGRGPVFRVLASCMHAGAKILEVRRSDGGKWLISVLAKFVEHESMNYASDGRPRYGVRGVEGFIVVSTSFYDKRLCVWKTKVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8E7</accession>
    <name>A0A8H6C8E7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147951.1">
        <property type="nucleotide sequence ID" value="XM_037296770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8E7"/>
    <dbReference type="GeneID" id="59334270"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="143" mass="16920" checksum="CA53174A5073C0EA" modified="2022-01-19" version="1">MERTWEQKKADEHEKAKEHERLDGEYQVLARETADLRSFLTHINRLLDDPDNDASQLEMQQDIKVTHERMISRNEDLMAEKMRFMKWLRKPDEDYMKWEDAPKAGSADESSAANKYESEMYDEGRSSFKVPEKNGGTEESSWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8G7</accession>
    <name>A0A8H6C8G7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148143.1">
        <property type="nucleotide sequence ID" value="XM_037296161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8G7"/>
    <dbReference type="GeneID" id="59333656"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Rhodopsin" evidence="6">
        <location>
            <begin position="32"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="143" mass="15766" checksum="539716A4C886D47A" modified="2022-01-19" version="1">MPDKELTGLFRDQFWLGKTQLSSGSESLQNSACIASIVRLVVLLDVDSEDLMYTSTLAQLWSCVEVSAGVISVCLPSLTTLFLLLLGKRPSNSTHHIISTDRHRDGKIENAECSRTLHATDETLWLQSLELMWASLLKSEGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8N9</accession>
    <name>A0A8H6C8N9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148478.1">
        <property type="nucleotide sequence ID" value="XM_037296496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8N9"/>
    <dbReference type="GeneID" id="59333993"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="64" mass="6116" checksum="6A2C0A4BFF8CF99B" modified="2022-01-19" version="1">MDKVGIGGLGNVLGDTLKGTTGTLGDATKGVGNVAKDTTGGVGDTARSATGAKQDAQNPLGLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8R4</accession>
    <name>A0A8H6C8R4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fumarylacetoacetate hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005604</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148495.1">
        <property type="nucleotide sequence ID" value="XM_037296513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8R4"/>
    <dbReference type="GeneID" id="59334010"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01299">
        <property type="entry name" value="Met_dep_hydrolase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000002">
        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057744">
        <property type="entry name" value="OTAase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="6">
        <location>
            <begin position="110"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="5">
        <location>
            <begin position="551"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="814"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1060" mass="114560" checksum="BB36A4539CCFB090" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8X0</accession>
    <name>A0A8H6C8X0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148464.1">
        <property type="nucleotide sequence ID" value="XM_037296482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8X0"/>
    <dbReference type="GeneID" id="59333979"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="69" mass="8267" checksum="B3EC4E77CB09E80A" modified="2022-01-19" version="1">MAAETYSTPIFVSCGYEPKRRTPAELLKRAKANRYKQEGHQEEDSKRDNTKYMKKTLRDRDIALRRYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C969</accession>
    <name>A0A8H6C969_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148458.1">
        <property type="nucleotide sequence ID" value="XM_037296476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C969"/>
    <dbReference type="GeneID" id="59333973"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="458" mass="50190" checksum="B68C234BE95C6D16" modified="2022-01-19" version="1">MSHYPPPPTPHLRRRGNDASGYGQASPNLHQAFMRTDPRQASSDRSKSSFDGRSSGRQASEQAVDEHDRRPRLTIQLPTPKSSPEPEVLQSPRDLARLSPVDAETALVDRFRKPVSKVSHPACLTPGQRSTTQDAEEAHPNEEQVNNANGPLATRARISDGDSPTLDLAARQLFQQAVPPGFNTNTDFGDFGRGLQPSAAAVAESSRRNRATPAHQRPHWVPTSEDYPSVPSNQGIGSRYPAEARRVPASRRANGRADAVQDESKGALGTEGDVAMRVEVILHGDGYRLRDSRHHPDIFQEIIPATPAVSNTADISETHLGYGRQAHRSRKTPSHHSESNADIASISSTECQPSTTASPPSFSPAFSPPPRVSCDIVARNPIPATSQRYFGPENGGRADIDDGEEATVVDIGENAGADARKRRLSLQMQEWRVWSKEPERPEKWSIDFDEVQHRGDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAR7</accession>
    <name>A0A8H6CAR7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003198</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149502.1">
        <property type="nucleotide sequence ID" value="XM_037294121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAR7"/>
    <dbReference type="GeneID" id="59331609"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF2">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="8">
        <location>
            <begin position="99"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="202" mass="22497" checksum="BD52EBEFBFE11CD4" modified="2022-01-19" version="1">MAGWTRAPFRRKHSEERAGNEISGQNQASYRLPTTIELEERVTSQVQEGRAEARPGRPRNATESPTTGIDTSHIKRLQHWWSHNVRLNLEHGAPGGDPRDYLALERTFLGWFRTSVALVSLGVVITQLFVLKDVHPRKGRTLGAILSCGGIVVVLLGCVRYLKQQRLLTQGKALSGGWHHQVLIITLLIVLITLFVVVVADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAV1</accession>
    <name>A0A8H6CAV1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003666</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149276.1">
        <property type="nucleotide sequence ID" value="XM_037294588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAV1"/>
    <dbReference type="GeneID" id="59332077"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="156" mass="17282" checksum="F431371769FC1673" modified="2022-01-19" version="1">MRTADGLLFLGCIEYHVPNTITTLYFHLGFPCKEAGVHNTINSARIYCEQKLEQGGDGPLPKSEDPFHEDLGYGAAINVVPSRPDYRLTWGILKGAMDGLWEFLVIEGRYVESEFDICHGALGLVGRGTIREAPETGLARQSRMREATNSFGTLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAX2</accession>
    <name>A0A8H6CAX2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_003331</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the methyltransferase superfamily. METTL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149635.1">
        <property type="nucleotide sequence ID" value="XM_037294254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAX2"/>
    <dbReference type="GeneID" id="59331742"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6220200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="359" mass="38966" checksum="CBA702AA52770A1B" modified="2022-01-19" version="1">MSQSFSFGFGSDDIEENGGEMADVGPLSLHDTRENKPVAVTEPKLHKLQDLLSALPSKISYRTVQIRSPAGILNVPRRELFDIRAQLMAEDEISDQSSPVVGLSTDDITPNVYEGGFKTWECAVDLAGHLFGSLSKGWELGGRVVHVVEVGAGTALPILVFFDFFLKQLSPSWRAVHLSVADYNLSVLANATLPNLLLTWYFVQSPAIPEPAGDLEITPDLLSRFVKDLSDKVIHISGISGTWNEAFTDLLLPVEDPQRRSRIETIFLASETIYSPNSIHAFTQVLLKALKSAEETHGHGRALVAAKKIYFGVGGGVDEFLKVLNELGGKGATAWESKDEGVGRVIVEVQSAGGNAAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB70</accession>
    <name>A0A8H6CB70_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149436.1">
        <property type="nucleotide sequence ID" value="XM_037294748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB70"/>
    <dbReference type="GeneID" id="59332237"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="86"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="368" mass="40100" checksum="20BE60EA4AA27DB4" modified="2022-01-19" version="1">MANGKSALDVCTPSLTPVTNAKLDNGKRDKGSLNEGVENVEYTLKPTTRAYASTARLNLQHYLWKDQTGYLLHPSIPLSGIENLKIADVGTGTGIWLLDLARTLPPTAQLDGFDIDISDCPPEQWLPRNVTMRHLDALGEIPEHLAGKYDIVQLRLFQVVVKDNDPGPLLRNMVKMLKPGGHLQWGEYDMTTHTTIKASPSLSTTALDAIPAFVQGFKKRDGRVGVQNWIPHLPHTFRTHSLTHTTSTRHATAPPYLPSQLDVYLLTYEELAAKTFDPMPGGHGDRLRELVAAAGEEGRRGVGWGMDRLVVVGRKAGGGGEDGGGSLVGEGGRDGETVVGGGWWNGGKGKRGGRRESLMRMWKRMSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBM2</accession>
    <name>A0A8H6CBM2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149778.1">
        <property type="nucleotide sequence ID" value="XM_037294397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBM2"/>
    <dbReference type="GeneID" id="59331886"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="947" mass="104252" checksum="CDA72A423937DB40" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBQ0</accession>
    <name>A0A8H6CBQ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="7">1.1.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003053</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:21252"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:16782"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the L2HGDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.2" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150055.1">
        <property type="nucleotide sequence ID" value="XM_037293977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBQ0"/>
    <dbReference type="GeneID" id="59331465"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF4">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="9">
        <location>
            <begin position="55"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="389" mass="42472" checksum="96FE7287291B847B" modified="2022-01-19" version="1">MFARALCRVLRMQCDLRPFSTSSTPRADFTHVVGAMLTATNSSRGETKTFQVVGAGAVIHAGLYYGPTSLKTHLCLKGKKMMYDLCEKQNIPYRNAKKWILSQNAQQHEELVKTHDFAKSIGVPIHFIPLSEAHAREPDVRVEEAVLESPTTGIVDSHTYMSYLESDFQSKGGDLACYTSVESVEPLASGSQGWKIQTKCPSSSKTTWITADTLINSAGLAAVSLSNSILPHARHVKPYYAKGSYYSYSAPMPRPKTLVYPAPTTGAAGLGTHLTIDMTGAIRFGPDVEWVDDPNDLSVNESRLPAALEEIETYLPRIQKEKVGLDYCGIRPKLGKGGAVWKGNGDGFLDFVIRKEEGFEGFVNLLGIESPGLTSSLAIAEMVDGLLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBY5</accession>
    <name>A0A8H6CBY5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149943.1">
        <property type="nucleotide sequence ID" value="XM_037293865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBY5"/>
    <dbReference type="GeneID" id="59331353"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="332" mass="37400" checksum="4B36A3B35D94C59D" modified="2022-01-19" version="1">MHIQSILDAWRLCSKRQLSKDLIQNHKPIVRKHLIQQETESCLGTHALHNPEQSTDKNGNPVEPLLRARALQGLPHQASGGSKIRPASFTPCRFYSNNRHKSFALTASEQLATSQSNCSKGQPESATQDSRGPETLTRRSQASHSHPNVQEPSSQAPHHAVSTSRITFSVVPKKISQVDFPAGRSAPTPPPPGPSPKRTAEDRRRSEAVLKTPIPLLRKYVSTKVIEKIKKSPYYVHDEDTIVLRCHQHQKQERNESLCNGQLFRLLENVEREIGYRESVGQIKDSQTVIVSENETEAMAWNLTKSFNIKMRQRKRKAKKAESLKALESPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBZ9</accession>
    <name>A0A8H6CBZ9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149856.1">
        <property type="nucleotide sequence ID" value="XM_037293778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBZ9"/>
    <dbReference type="GeneID" id="59331265"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="66" mass="6871" checksum="C469C9B0B8A61CAB" modified="2022-01-19" version="1">MTDVNARGGYNGDALLAASVSGHLQTTPAQLREPWGENDAADAPELEALEEEALEEESAGASSEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCQ2</accession>
    <name>A0A8H6CCQ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">DNA replication complex GINS protein PSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_002733</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the GINS1/PSF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150487.1">
        <property type="nucleotide sequence ID" value="XM_037293658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCQ2"/>
    <dbReference type="GeneID" id="59331145"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11710">
        <property type="entry name" value="GINS_A_psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1030:FF:000003">
        <property type="entry name" value="DNA replication complex GINS protein PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005339">
        <property type="entry name" value="GINS_Psf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914:SF2">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914">
        <property type="entry name" value="PARTNER OF SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="9">
        <location>
            <begin position="57"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="208" mass="23474" checksum="2A0FF22C23E182AE" modified="2022-01-19" version="1">MYGDDAFKLVQHAKRTQALVHLPHYQTELVRETTREVRDLDKDVSNILAPFEGSFNPSSEPATACSVLVNHLCMRRNKRCLLAYHRVRSDKLEEMCWSGKDILDQEQQAGRQGNGEGSGMASEAGNQSSLSPEEEEYIRQYGDMLAAYKGQWTDIDLTGSLEPPRDLFIDVRVLKDAGEIQTEYGAITLTKNSQFFRQAPSTYVKVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCU3</accession>
    <name>A0A8H6CCU3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150319.1">
        <property type="nucleotide sequence ID" value="XM_037293490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCU3"/>
    <dbReference type="GeneID" id="59330977"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="549"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="562" mass="57419" checksum="774B60BF2A433D11" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDZ9</accession>
    <name>A0A8H6CDZ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial</fullName>
            <shortName evidence="7">Glu-AdT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001680</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151147.1">
        <property type="nucleotide sequence ID" value="XM_037292608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDZ9"/>
    <dbReference type="GeneID" id="59330094"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="67"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="508" mass="54222" checksum="96EF3D879102FECA" modified="2022-01-19" version="1">MSLLRRAETCFANQQVNKLLNAFVCPRDEGTLISEHTSNLRDADKRRDRGTSIKESLGKAYLIALIGTPKSAIDGCLIAVKDNICTSEEPTTCASAILKGFHSPYPATVVEKLKHAGALIAGKTNLDEFGMGYTQDGGSLSAGGSSGGSAVAVATGQCDAALGTDTGGSVRLPAAYTGIIGFKPSYGMVSRWGIVAYANSLDTIGILAKDSSMARNIFDAVEGFDSRDPTSIPVSTRSRIAQQLLERKAKQGWGDTPSVRIGVPREYNTAELQPEVRRAWLETLQGLRNQGHTIHMLSLATTKMALSAYYVIAPAEASSNLAKYDGVRYGNKNSASERADNVLFASTRGAGLGEEVKRRILLGSYSLSAAAIDNYFKTAQKVRRLVQRDFNNAFALSHPLLKRQDGSGSEPKVDVILTPTAQSLPPRHSSLKNKASIDSYSVDFLTVPASLAGIPALSLPVSIAGQDGIDQNAPTSVGIQVIAQYGDEEMVFAMARSIESLRDIKQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE92</accession>
    <name>A0A8H6CE92_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001802</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151269.1">
        <property type="nucleotide sequence ID" value="XM_037292730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE92"/>
    <dbReference type="GeneID" id="59330216"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="116"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="309" mass="32452" checksum="2AF08B6DDB807E98" modified="2022-01-19" version="1">MAQYYPQQQPPYNPQNLQFYQSTYPAPVSGHSTPSQAHYGYGQPSAPQPYPSSGGFGAAPSFGGQGVSGRMGEQGGLRTGWIAAFGTEGYDGEPPLLEELGVNFGHIKGKTLTVLNPLHPISQHIMDDSDLAGPILFFLLFGTFLLFSGKVHFGYIYGLALVGSLSLHTIFSLMSPPPEGNSPTSTPSYAQPTHDHMSGAGGMGGGGQLSSTLTFPRSASVLGYCLLPLVLTSFVGVVLPMDTAAGYVISSLAICWCTYSSSKMFCAVGRMRSMRGLVAYPLGLFYVGFGIMGIFSSKGTGGLGRVATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CED4</accession>
    <name>A0A8H6CED4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151280.1">
        <property type="nucleotide sequence ID" value="XM_037292741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CED4"/>
    <dbReference type="GeneID" id="59330227"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021861">
        <property type="entry name" value="THO_THOC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265:SF0">
        <property type="entry name" value="HPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265">
        <property type="entry name" value="THO COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11957">
        <property type="entry name" value="efThoc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="639" mass="71991" checksum="CF02A6154816C662" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEE5</accession>
    <name>A0A8H6CEE5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Epoxide hydrolase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001981</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6222013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151448.1">
        <property type="nucleotide sequence ID" value="XM_037292909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEE5"/>
    <dbReference type="GeneID" id="59330395"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF35">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="411" mass="46503" checksum="37F16C1BB1F9B488" modified="2022-01-19" version="1">MASIEPYTISVPEEQRQQLTQKLEATTFPDELDQAGWDYGAPLADVKRLVKYWREKFDWRKQEAKLNELPNFKTAIQVDGFESLDIHFVYQKSDVEGAIPLLFSHGWPGSFIEVTKILPLLANGGKEAPAFHIVAPSLPNYGFSSGTKKKGFGLPQYAETCHKLMLKLGYDKYVTQGGDWGCMITRIMGLLYPQHVMASHINMVRGHAPEFLSHPILATQHALAPYTQREKDGHARSKWFLEEGSGYRLEQSTKPQTLGYALADTPVGLLAWIYEKLHDWTDSYPWTEDEILTWVSIYWFSTAGVAANLRIYYEAMHDTQFGRERTEKWIAHVKLGLAHFPKELTVVPKSWGRTMGPVMYESQNESGGHFPAWEKPEAIARDLQEMFGKGGGAFGVVKGKVGYLSPRTAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEG8</accession>
    <name>A0A8H6CEG8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001694</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151161.1">
        <property type="nucleotide sequence ID" value="XM_037292622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEG8"/>
    <dbReference type="GeneID" id="59330108"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000244">
        <property type="entry name" value="Nonsense-mediated mRNA decay protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="24"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="897"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="955"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="907"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="962"/>
            <end position="973"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1046" mass="118981" checksum="18AF660507259434" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CES8</accession>
    <name>A0A8H6CES8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial distribution and morphology protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mitochondrial inheritance component MDM10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MDM10</name>
        <name evidence="7" type="ORF">HO133_001253</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis and may function in phospholipid exchange. MDM10 is involved in the late assembly steps of the general translocase of the mitochondrial outer membrane (TOM complex). Functions in the TOM40-specific route of the assembly of outer membrane beta-barrel proteins, including the association of TOM40 with the receptor TOM22 and small TOM proteins. Can associate with the SAM(core) complex as well as the MDM12-MMM1 complex, both involved in late steps of the major beta-barrel assembly pathway, that is responsible for biogenesis of all outer membrane beta-barrel proteins. May act as a switch that shuttles between both complexes and channels precursor proteins into the TOM40-specific pathway. Plays a role in mitochondrial morphology and in the inheritance of mitochondria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. Associates with the mitochondrial outer membrane sorting assembly machinery SAM(core) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion outer membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="6">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="domain">
        <text evidence="6">Lacks alpha-helical transmembrane segments, suggesting that it resides in the membrane via beta-sheet conformations similar to those predicted for other outer membrane proteins and porin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MDM10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CES8"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051654">
        <property type="term" value="P:establishment of mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03102">
        <property type="entry name" value="Mdm10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027539">
        <property type="entry name" value="Mdm10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28035">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28035:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12519">
        <property type="entry name" value="MDM10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1 6" id="KW-1134">Transmembrane beta strand</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="416" mass="46327" checksum="96E1E82458981F7F" modified="2022-01-19" version="1">MLGFMDYVQNAFYEASRWDRDNSYGTLTATSRNILDFSTPEGLRFHVSSLSSPNFATSYTLGSIGLVDGSLSYLYSSLPLPRLAAKSSAIDLHHLVPGYRQLQDLRQPDEPWWWEIWHQGKRIDRREALLYGRLFLPRSTLEALYLRRLSPKTQLKVSCVSDSRLPNGGTILAHLQHDAAKHNTEYIYSTDSALFGVRGLYNFGPDPAPTRPLFEASPDSTISTANEAAAFTTAQTPSQPHGRFSAGGEIYYGLLNKSGGISTGLRFATLPSHTGFPYTMTLTLNPLMGNLSSTYSVLASPLLALSSRFDFNVYSYESELQVGTELWRRKEKPLIEDLNWARLKMGQKVLNDPSKDLDNPNGMAGCFKARVDQRGGLGLLWEGRVKELLYSCGATIDLRGGENMFRGFGVEVQYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFE3</accession>
    <name>A0A8H6CFE3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001456</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151805.1">
        <property type="nucleotide sequence ID" value="XM_037292386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFE3"/>
    <dbReference type="GeneID" id="59329872"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF93">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 25D9-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033993175" description="Integral membrane protein" evidence="8">
        <location>
            <begin position="19"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="538"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="544"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6222370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="62898" checksum="4C280F2E85F7EF24" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFS7</accession>
    <name>A0A8H6CFS7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001517</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151866.1">
        <property type="nucleotide sequence ID" value="XM_037292447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFS7"/>
    <dbReference type="GeneID" id="59329933"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01786">
        <property type="entry name" value="RA_STE50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09533">
        <property type="entry name" value="SAM_Ste50-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051569">
        <property type="entry name" value="SHANK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135:SF28">
        <property type="entry name" value="LD13733P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135">
        <property type="entry name" value="SH3 AND MULTIPLE ANKYRIN REPEAT DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="67"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="4">
        <location>
            <begin position="431"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="252"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="390"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="554" mass="60748" checksum="3FF04603BFE05538" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG34</accession>
    <name>A0A8H6CG34_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SURF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000981</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probably involved in the biogenesis of the COX complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SURF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152276.1">
        <property type="nucleotide sequence ID" value="XM_037291914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG34"/>
    <dbReference type="GeneID" id="59329399"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06662">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002994">
        <property type="entry name" value="Surf1/Shy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045214">
        <property type="entry name" value="Surf1/Surf4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427:SF2">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02104">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50895">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="314" mass="35990" checksum="769897EA38B956E4" modified="2022-01-19" version="1">MKPSLFASPFSKSTRLTSLRTALRPRICPKCEQPSPFQRRYAHTPADDPYFQSIVDNPAVPVRSGQKHGPGLIVLILIPAIAFGLGTWQVFRLRWKTDLIARFEDRILKPPLPLPPRIDPAAIKDFDYRRVVTTGLLRHDQEMLIGPRVHDGNDGYLVVTPLQREGIASTILVNRGWIPKKFKRQLDRQAGLPIGEVTVEGLLREPWKKNMFTPDNKPELGEFYFPDVEQMAKFTGSDPVWIEETMVPDLLKAYDREAVGIPIGRPAEVNLRNNHTQYIFTWYALSAATAIMFWMVVRKPPSDSIRRVRQNKEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGS5</accession>
    <name>A0A8H6CGS5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152461.1">
        <property type="nucleotide sequence ID" value="XM_037291026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGS5"/>
    <dbReference type="GeneID" id="59328505"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="276" mass="31422" checksum="BF0D055967ECAC3B" modified="2022-01-19" version="1">MAGNSWSKVPREIRDQIYINMVQGKKYVLVDAEGSPDKPLSAFYYRELDYSPPAYAGLLRASKTISNELQEVLYKECVFRAYLSKHRYRLVTPPEPREFARIQKMEIVLDNAIYLANPITLPGTDVRGFDSYYGEWFEALGGSGADHEFCRIRIINIAPETFYLTPVCRQLLYALKSFTGFGTVIVELGDLPDSQAIQLPASHGANPQVKDEPPEEPMYAGARFEALKRTIKIELEPYLGNCIYYDRGLLRCVEFRPRTDDPIPPGCAPYIEAWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGT3</accession>
    <name>A0A8H6CGT3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152247.1">
        <property type="nucleotide sequence ID" value="XM_037291885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGT3"/>
    <dbReference type="GeneID" id="59329370"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034772">
        <property type="entry name" value="CPSF6/7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23204">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="42638" checksum="2D7AE04EEBD76DBD" modified="2022-01-19" version="1">MAAEEDNFDIDIYGDGGEDDQNQEPEDGMKAEESSDQTLQPPEPASNAADHSSANDQRVNSDVKIEHEGNDVAQKIASTDESGSDPVQLPKQAPQTQGLKRKDGVDDRYVDPGATTALFVSELHWWVNDDDVRGWANQSQCEDELEDMTFSEHKVNGKSKGQAYVLFKTPQAATAAKHKIESFGEGQQYTKKFTVSYTNPYTNPFRTLPKDGPARNNAPVNNNRSTSGSYGGAGAGGTGGTPQSFNNGSGGYRGNRGGGYNNRGGGMNNMSGYSRGGFQQPVTGSFQGSGMGGFQGSPMGGMQSYGGFQTRGAMMGGMRGGPMGMRGGRGGINPSGIMGMPMTGMGMSAMGGMGMSMPQMNGGMGMQAHYNPAFFTQQQQGQQGGSSGDANWNPHGAKRTRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH07</accession>
    <name>A0A8H6CH07_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152455.1">
        <property type="nucleotide sequence ID" value="XM_037291020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH07"/>
    <dbReference type="GeneID" id="59328499"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034739743" evidence="1">
        <location>
            <begin position="23"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="257" mass="27151" checksum="0FC6535191F1C640" modified="2022-01-19" version="1">MLSLRIAFLHLVAFSAITTTNAAPEATATNTVNLRIEANTSTLYEGPITSGPRNITVGSGNNKVTFICDGAGAMPSPPPPGNTPTDALDAAAKARGFTYDGGFDGEFNDFYFTRIGTSTEYDNGTYFQSWGSLVNYQVSKFGEGLTLSGCQQLVKAGDEVLWAFITVKGTDSSNTDPSVLFLKLAPTAVTVKKGKGTTVTVTDGRSGVVVQNASVAGVKTDANGKATLYFFETGFFQYKAHKTGDVRSNVMNVTVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH62</accession>
    <name>A0A8H6CH62_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152502.1">
        <property type="nucleotide sequence ID" value="XM_037291067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH62"/>
    <dbReference type="GeneID" id="59328546"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF39">
        <property type="entry name" value="AMINOTRANSFERASE CLASS I_CLASSII DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="2">
        <location>
            <begin position="81"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="483" mass="53275" checksum="5389DCD966A53DBC" modified="2022-01-19" version="1">MEPEIPNAEALTPELSKPSMARLSNRGVANMVPDAIKAIMKAGSSNPHHPQHNADGIVSLGVAENRLLHAGIAQHVSSHMHVSQRMLTYGDGSVGSDVLRQAIADFINDRFQPVEPLGYSSVTVLNGVSAVLDSLAWCICDEGDSILIGKPFYVGFVSDFMNRAKVDLVAVTFGEVDPMSLAAVAHYERAMKEAVTRGKKLKALVLCSPHNPLGRCYTPEVLEAYLRLCSKYWIHMISDEVYAMSVFPNGDIPNPVPFRSVLSLEYRKLIDPSLLHVLYGMSKDFCANGLRIGAFISTTNPELHAAMRAISKFAWSSSLADLAWSSILSDGTFLQEYLIKITARLTEAYNLCTSILRGMNVPYLPANSGPFLWINLSGFLEHKTIESERDLAWQMIKAGVWLATGEAYRSEEPGWFRLTFAVSETEIRMGLERYVEQLVGYGGIASWLIWRLQTCACSGTGQHCNSLKPDMREPLGARDPESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHW9</accession>
    <name>A0A8H6CHW9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-dehydroquinate synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000576</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152950.1">
        <property type="nucleotide sequence ID" value="XM_037291515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHW9"/>
    <dbReference type="GeneID" id="59328995"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017000">
        <property type="term" value="P:antibiotic biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08199">
        <property type="entry name" value="EEVS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1090.10:FF:000015">
        <property type="entry name" value="3-dehydroquinate synthase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000018">
        <property type="entry name" value="Related to 2-epi-5-epi-valiolone synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050071">
        <property type="entry name" value="Dehydroquinate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035872">
        <property type="entry name" value="EEVS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43622:SF3">
        <property type="entry name" value="2-EPI-5-EPI-VALIOLONE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43622">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24621">
        <property type="entry name" value="DHQS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-dehydroquinate synthase N-terminal" evidence="6">
        <location>
            <begin position="84"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="3-dehydroquinate synthase C-terminal" evidence="7">
        <location>
            <begin position="195"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="52485" checksum="31A3D8B8BBA74D10" modified="2022-01-19" version="1">MSDFTASVVETKNGFHVDGYEKISYDFTFLDGVFNPENPQLAECYKRWGRVLAVTDLNVYNVYGEQIERYFEHYGLELKLHKTKIGEKAKTIPTFLSIVDSMTEFGIYRKEPVLVVGGGLVTDVAGFACAAYRRNTNFIRVPTTVIGLIDASVSIKVAVNYGNYKNRLGAYHAPVHTFLDFTFLRTLPTAQIRNGFAELIKISTCSHLDTFDLLDKYCEQLIDTGFGRTDGAPEEVRVAADKINKEGIYEMLKLETPNLHEIGLDRVIAYGHTWSPMHELVPETPLRHGHAISIDMAYSATLANSRKLISDAEHRRILNLFSRAGLSMDHPQFDEEILDKGTAAILKTRDGKLRAAVPNPIGSCTFLNDVDAEELNAALRRHKELMREYPRNGEGLEAFVDASDTGYTENNKSEVKAVEAAAAKAGSLNGNGAIHGGIVRNGHMNGTNGVKEKLANGSGAVDGLTNGINGYTNGSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ92</accession>
    <name>A0A8H6CJ92_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010942</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153425.1">
        <property type="nucleotide sequence ID" value="XM_037301791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ92"/>
    <dbReference type="GeneID" id="59339332"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24162">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013881">
        <property type="entry name" value="Pre-mRNA_splic_Prp3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027104">
        <property type="entry name" value="Prp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212">
        <property type="entry name" value="U4/U6-ASSOCIATED RNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08572">
        <property type="entry name" value="PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06544">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor 3" evidence="7">
        <location>
            <begin position="186"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Small nuclear ribonucleoprotein Prp3 C-terminal" evidence="6">
        <location>
            <begin position="425"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="144"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="152"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="568" mass="62562" checksum="56BA58C37D254DCB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJI8</accession>
    <name>A0A8H6CJI8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Exonuclease V</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010926</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EXO5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153409.1">
        <property type="nucleotide sequence ID" value="XM_037301775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJI8"/>
    <dbReference type="GeneID" id="59339316"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045145">
        <property type="term" value="F:single-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019190">
        <property type="entry name" value="EXOV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14464">
        <property type="entry name" value="EXONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14464:SF4">
        <property type="entry name" value="EXONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09810">
        <property type="entry name" value="Exo5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="58764" checksum="012024BED6B81F38" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJM3</accession>
    <name>A0A8H6CJM3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Triosephosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009922</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153596.1">
        <property type="nucleotide sequence ID" value="XM_037300779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJM3"/>
    <dbReference type="GeneID" id="59338315"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF2">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="259" mass="27650" checksum="3CBF39037AD2E019" modified="2022-01-19" version="1">MKPPTLPERLVGVNLKMYFDAPSTTAYITAIARFVAPPASISTGIFILPAYPCLASASDLLDMTPQVILGAQNCHAEDSGAYTGEVSPLMLKQLGCRVVCLGHAERRRAPFNETDEVVATKAEAVVRNGMIPLVCIGEKSRSGIMSEGVGIAVRECSPQVMSVLRAVPGDAPIIFAYEPVWAIGAQEPASADHVLAVVKSLKQLIGTVEERADVRILYGGSAKPGTWGTLKEGVDGLFLGRFAHDVGNFERVVREVEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJU3</accession>
    <name>A0A8H6CJU3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NOT2/NOT3/NOT5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009977</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153650.1">
        <property type="nucleotide sequence ID" value="XM_037300833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJU3"/>
    <dbReference type="GeneID" id="59338369"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="558"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="467"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="242"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="392"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="420"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="691" mass="76071" checksum="08806BD5505D04C2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK34</accession>
    <name>A0A8H6CK34_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Xaa-Pro aminopeptidase</fullName>
            <ecNumber evidence="5">3.4.11.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aminoacylproline aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_010193</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.</text>
            <dbReference type="EC" id="3.4.11.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153865.1">
        <property type="nucleotide sequence ID" value="XM_037301048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK34"/>
    <dbReference type="GeneID" id="59338585"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF1">
        <property type="entry name" value="XAA-PRO DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="12">
        <location>
            <begin position="12"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6224998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="463" mass="52611" checksum="945AF55E6F66C550" modified="2022-01-19" version="1">MKHHTSILKGKYPAKAHCAKVAAHLKETVKDDSPARIYIQGQKTRMIEDNDEPQPFRQRRHFFYLSGCNLPDCHLVYDIPTSTLTLFIPPLDPSSVIWSGLPLSPDQALHKYDVDQVRPSSELYDHLAPTRRPGSSAPLTFATKSHLPCDQYEQSYFVPYNLDALHTAVDECRVTKDVYEVAMLQKANEVTTAAHIAVLKNLKHKRNEQELEAVFTERRIALGCTEQAYHGIFGSGHNAATLHYVANDQHLQGRWNLLVDAAAEYDCYCADVTRTMPLNGTFNEESEGIYRIVQKMQEECLGMLKAAVTWEDVHVKAHEVAIEGLLDLGLLKGDKKDIFDSRTSVAFFPHGLGHYLGMDTHDTGGHPDYEDKDKMFQYLRVRGTLPEGSVITVEPGVYFCRFIIEPYLKDEKHKEFIDEKVLERYWNVGGVRIEDDILITKDGYENLTTAPKDIDEMKKIINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKB5</accession>
    <name>A0A8H6CKB5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153668.1">
        <property type="nucleotide sequence ID" value="XM_037300851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKB5"/>
    <dbReference type="GeneID" id="59338387"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="265" mass="29545" checksum="F9E7817FFB5099B7" modified="2022-01-19" version="1">MSSQYLKKVKSQGMEGKLSEAIDGTMNTSEDNLNDSLDDVLDPDRTSTNGEITDLYHLRKKAKVAHAPSSSFEASSNKTDSADSNTLNSSIYSNDYNAANILQSSQSRICPEKLSSGRQSTTAPEEYLAKTVHSISEYEASDDELLSPPASETFHAKYRKSSPDLMTLHMFTGKMNGKTPIYVPVYKRNNFGKLLKLMERWSGHWEYMELKKLTEEELDAGLKTLEKPKAEVVVQSSEPKKAVGGRKLADFSLRETKSNGGRDWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLD4</accession>
    <name>A0A8H6CLD4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009507</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154216.1">
        <property type="nucleotide sequence ID" value="XM_037300368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLD4"/>
    <dbReference type="GeneID" id="59337902"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015244">
        <property type="term" value="F:fluconazole transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF23">
        <property type="entry name" value="FLUCONAZOLE RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="488" mass="55033" checksum="4103D1A027D9A490" modified="2022-01-19" version="1">MADLFREAPFGQLVRIPSGNKFFRYPEEREDFHCPHCYHADASLPSEKIVEQSSNSEVNGVGSFKATRTSTLDTKLDSERSATSSDPDVEDDQEQEPARALGLQRTQKLPYTNERLEIGKKLEVERTKFRPIRFGVNESVASLGLALYVLGYGTGPLVKLQLSAETYLTFSRFWQGFFGSPCLANGGATMQDMYSLLYLPFFVSVWVSAILAAPALGPILSGFAVVVENWRWSLWEILWLAGPVFVLWFFCLPETSASNILLRQSMRLRKLSQNPNISSQSQTDRRGLTASAIALDALIKAFEIMFKDPAVLFTNVYTALIYGIYYSFFEVFPLVYGPLYGFNIGETGVVITCIIVACVLAMGIYFYYLQYMLIPDTSKNGLRAQESRLRLALIFVWGPLIGPFILVNSFLRTPRLEVAHQRLLLGWSANKNVHWIVSVIGITIYALTVYIISQCLFVYIPNGFYDLPTSRPRSDRNPHGAPALFLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLQ1</accession>
    <name>A0A8H6CLQ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_009716</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154424.1">
        <property type="nucleotide sequence ID" value="XM_037300576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLQ1"/>
    <dbReference type="GeneID" id="59338111"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058545">
        <property type="entry name" value="Beta-prop_EMC1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25293">
        <property type="entry name" value="Beta-prop_EMC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034491597" description="ER membrane protein complex subunit 1" evidence="13">
        <location>
            <begin position="23"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="953"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="domain" description="EMC1 first beta-propeller" evidence="15">
        <location>
            <begin position="22"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="14">
        <location>
            <begin position="745"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="784"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="794"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6225715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="984" mass="105756" checksum="9BA81FF65A2D6D72" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN61</accession>
    <name>A0A8H6CN61_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154724.1">
        <property type="nucleotide sequence ID" value="XM_037299898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN61"/>
    <dbReference type="GeneID" id="59337432"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF5">
        <property type="entry name" value="BTB_POZ DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="211"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="407" mass="44473" checksum="0738BD63EF96DA01" modified="2022-01-19" version="1">MSSQPNRGRPVRPQPPTTAPPMIQAQQPQSYFTPNPYGPHPTQLQQPFQPPPGPPPQQLLAPQPQPPPQAGSPPSRKRKAPGSAPLAAMPPMPSTSLGEPNDGGQQVEEANEASSSMPPPKKSRTNTPWTPAEEQRLKIMRDAGNSWGEIAKDMHYADFAEDDSAALLAAIKEYEASKWKVIGQKVGKPAKVDRLSHSASPFILWKSKEGFDLTLACGFITLKVHSFLLKPRSTFFAATREGDFLEATSKPIALPDDNVESVAQMVQYCYTMDCPDEKPEKILDPGHPTTPYISRAQTNAQVHAPAEKYDIPGLKFLAGMKFGAAMAVEVGPGFRSGAPAMMHLIAIISLVYDSAPGTDRVFRDAVIGYMWMYWKQLSPKPEFLVPIAAKPEFMIEAVNKTFGPQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP90</accession>
    <name>A0A8H6CP90_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155497.1">
        <property type="nucleotide sequence ID" value="XM_037299496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP90"/>
    <dbReference type="GeneID" id="59337026"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="831" mass="89157" checksum="11F236E52AD6FE6A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPZ0</accession>
    <name>A0A8H6CPZ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Diacylglycerol O-acyltransferase</fullName>
            <ecNumber evidence="5 16">2.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_008793</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Endoplasmic reticulum membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.20" evidence="5 16"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155657.1">
        <property type="nucleotide sequence ID" value="XM_037299656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPZ0"/>
    <dbReference type="GeneID" id="59337188"/>
    <dbReference type="UniPathway" id="UPA00282"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF0">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6227349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="473" mass="52804" checksum="F7C6940D62B2A337" modified="2022-01-19" version="1">MATAATLNQEPFLPAEPKQQHLSPNSYVDAAGGNVDTFSQNGRPPPELYSGQGEDATPRSPRRTSHKKSTSLKTNGISKEKKDGQVVVERYEDKDGEHLVSIRQAWDRGKGKPMAARRNSELVSGRKAGAGWEQSHIHFAPLSVPLKRRLQTLVVLGHTLSIALLLGTFFLLCAIPLFWPILVPYLIYVLFSNAGHSGTLSHRSNFLRSLPIWSLFASYFPARLHRSALLAPTRKYIFGYHPHGIISHGAFAAFATEALGFSQLFPGITNTLLTLDSNFRVPLYREYTLSMGLASVSRESCENLLSRGGANGEGMGRGITIVVGGARESLDAQPHSLRLVLKRRKGFVKLAIRTGADLVPVLAFGENELYDQVQPEQHPLIHKIQLVIKKVLGFTVPLFHARGVFNYDVGMMPYRRPLNVVVGRPIKVQQASKPEETYVDELHDVYVKELERIWEEWKDEFARERTGELQIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQE0</accession>
    <name>A0A8H6CQE0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D-arabinitol 2-dehydrogenase [ribulose-forming]</fullName>
            <ecNumber evidence="5">1.1.1.250</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007668</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; D-arabinitol metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.250" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155874.1">
        <property type="nucleotide sequence ID" value="XM_037298538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQE0"/>
    <dbReference type="GeneID" id="59336065"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047038">
        <property type="term" value="F:D-arabinitol 2-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000240">
        <property type="entry name" value="SDR family oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF4">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="303" mass="31864" checksum="1A62AACDF674080A" modified="2022-01-19" version="1">MPSPSNTTQDDLRARSIPHMTLSTGDTSSMTGPPPPMPHYLTSEGRAIKRFAVEGNAIFTGGAGTLALVNARALLEHGLSGLALFDLDHSKSAQEIQALKADFPNARVLSIKVDATNAEQLGSAVKLTVSELGSVDILCCFAGVVGCTHAIDMAPSEWKSRLEINTTGSFLSAQAAARQMIAQNTGGSILFLASISAHRTNYPQPQAAYNASKAALLSLKYSLAAEWAVHGIRVNSVSPGYMDTVLNEGSGLEEARRTWGERNPMGRMGVPEELTGVVVLLCSQAGGYVNGADLVVDGGQCCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQZ5</accession>
    <name>A0A8H6CQZ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-oxoisovalerate dehydrogenase subunit alpha</fullName>
            <ecNumber evidence="9">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_007696</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the BCKDHA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155902.1">
        <property type="nucleotide sequence ID" value="XM_037298566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQZ5"/>
    <dbReference type="GeneID" id="59336093"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000015">
        <property type="entry name" value="2-oxoisovalerate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="10">
        <location>
            <begin position="91"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6227968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="444" mass="49909" checksum="A7EBCB564604F1E5" modified="2022-01-19" version="1">MLLRNPLRAQILQRVPRRQTRWSSLSQRPGSDRVHFPGAVSSKFTTKLSFEHPSTHPAMPTYRVMDSDGVVVDESRKPSGISNEEIVTWYRNMLTVSIMDLIMFDAQRQGRLSFYMVSAGEEGIAIGSASALVPEDVIFCQYRETGVFKQRGFALSDFMNQLFANCKDAGKGRNMPVHYGSGKLNIHTISSPLATQIPQAAGAAYALKIQALQNPDVPPRVVACYFGEGAASEGDFHAALNIAATRSCPVVFICRNNGYAISTSTLEQYRGDGIASRGIGYGIDTIRVDGNDIFAVREVTKEARRMALEDGGKPVLIEAMSYRVSHHSTSDDSFAYRAKVEVEDWKRRDNPITRLRKWMQHQGMWDEEKEKEARSQIRKDVLKAFAEAEKEKKAPLRDMFTDVYEDLTEESRGHMKELGRLLDDYPEEYDVNEFEGGRKGLSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR95</accession>
    <name>A0A8H6CR95_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007954</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156158.1">
        <property type="nucleotide sequence ID" value="XM_037298822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR95"/>
    <dbReference type="GeneID" id="59336351"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="276" mass="30028" checksum="523C023FF2A50490" modified="2022-01-19" version="1">MSVTKPQILLLQLGEAYEKEIFDDLYSGLCTKIEEHYTVIKTSSLTTEHLAHSEAIVVTDGGLSRKKHRTTIQVRLSEYAKAGGTVILACLFSSFVSGPEFAGMCRSMNLPWDWGDYHRTVFALNPAFANVFDNEAFKTLEQSYSMKAVHLGNVHAADKVYVPTDHSRVQSAVFPPDRVDTAQTPAVWQKHGQGHIAYIGDVNNESGSQALIMAMLGIAAKGGPRQAVAEENADLPALVSGCEMCEGGLEISQGALPESSIINRALIVEYMCTMGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRN4</accession>
    <name>A0A8H6CRN4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PXA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008164</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156368.1">
        <property type="nucleotide sequence ID" value="XM_037299032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRN4"/>
    <dbReference type="GeneID" id="59336561"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000065">
        <property type="entry name" value="PX domain protein (AFU_orthologue AFUA_2G07450)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF47">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 122 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PXA" evidence="3">
        <location>
            <begin position="189"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="400"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="454"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="659"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="720"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="827"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="455"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="724"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="747"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="847"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="889"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1070" mass="115522" checksum="E5296321EBCFF96C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRP7</accession>
    <name>A0A8H6CRP7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nab2-like CCCH zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008043</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156247.1">
        <property type="nucleotide sequence ID" value="XM_037298911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRP7"/>
    <dbReference type="GeneID" id="59336440"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.40:FF:000001">
        <property type="entry name" value="Nuclear polyadenylated RNA-binding protein nab2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.40:FF:000002">
        <property type="entry name" value="Nuclear polyadenylated RNA-binding protein Nab2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.40">
        <property type="entry name" value="Nuclear abundant poly(A) RNA-bind protein 2, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055046">
        <property type="entry name" value="Nab2-like_Znf-CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040366">
        <property type="entry name" value="Nab2/ZC3H14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043094">
        <property type="entry name" value="Nab2/ZC3H14_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738:SF29">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22683">
        <property type="entry name" value="Nab2-like_zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nab2-like CCCH zinc finger" evidence="9">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="315"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="541"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="316"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6228313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="564" mass="61772" checksum="37CA5482AAE9FDC4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6X3</accession>
    <name>A0A8H6F6X3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PKS/mFAS DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006916</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000028">
        <property type="protein sequence ID" value="KAF6217446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146881.1">
        <property type="nucleotide sequence ID" value="XM_037297812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6X3"/>
    <dbReference type="GeneID" id="59335316"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.250">
        <property type="entry name" value="Malonyl-CoA ACP transacylase, ACP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF29">
        <property type="entry name" value="ASPERFURANONE POLYKETIDE SYNTHASE AFOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="3">
        <location>
            <begin position="300"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="380" mass="42207" checksum="6CE5368A3BA99E0D" modified="2022-01-19" version="1">MIAISMLVLFGTDGCSYNYDHRAIGYRGGGHMIFLKPLEVLQSNDQNGLINLANINQPATTAIQIALVSLLASWNINPAAVVGHSSGEIAAAYAVGAVSLFSCTAVAYHRGGFASKRAARDAVDSLQKLATDADVFVRRLKVDIAYHSHHRRDIEAEYPITLGGIRPNRALSQAHVFFLTERQASSLGDLEAEYWCQNSTSLGLFSQITTKMCWGRNDDVSESNIDLIMGIGPHSSLKAPGDPQLQKSYSGSSPRYLSTLLRDSDAAPSLLGTAGALHCTEFPLEFRDLKNNRFRRFPGHELFGVLVEDFEESEPRWRRVIRLSEIPWLSHHLTQFNVVFLFAAYVSMACEVVFQRATMRDHKVGESHSYNFRVFSVKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F715</accession>
    <name>A0A8H6F715_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146941.1">
        <property type="nucleotide sequence ID" value="XM_037297740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F715"/>
    <dbReference type="GeneID" id="59335244"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="2">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="292" mass="31944" checksum="2D8358AA964B76BB" modified="2022-01-19" version="1">MIVPPKLPSKHDAAVKLLPSEANSTRKNPATDLNHVLDKLHHTTELQHVSQAPKIEQPQKNVSDAAMTKVHRDPKSLQVAEPKTLLRAAGQSVSGHKAELLDRLMGVGDDDDYFSIDEDGNRLPPRDMNPQLDPASLVGHRIQRFQISADPGSVLPTDQGPVAITFPGVAFSKYADSAADAAFAGGTRVRRASDRNVVPRLARAERETREKKEKRSADRKRGGRRLEGRVRTSGRTGSWGSGARGCGRWGVPCSRRMTCGMFGLLRAGAQVRGCDVAEAWTGRGVRKPEWER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7F7</accession>
    <name>A0A8H6F7F7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006856</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146953.1">
        <property type="nucleotide sequence ID" value="XM_037297752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7F7"/>
    <dbReference type="GeneID" id="59335256"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000025">
        <property type="entry name" value="40S ribosomal protein S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7684" checksum="7E4955881D8267A5" modified="2022-01-19" version="1">MDSGKIPVKLVKVTRVLGRTGSRGGVTQVRVEFMDDTTRSIIRNVKGAVREDDILCLLESEREARRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F822</accession>
    <name>A0A8H6F822_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006062</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147539.1">
        <property type="nucleotide sequence ID" value="XM_037296965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F822"/>
    <dbReference type="GeneID" id="59334467"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="148" mass="16001" checksum="D54362DF907953ED" modified="2022-01-19" version="1">MFQQGWTTGALLFEKAAVCNGPASLAEKLSAIPKWERPASPLTDYTDSWFGRLLSIRPLNGTASDDIVVSDDANAYIESRPGGLSHPRNTLFNNSVGELLAAKPTSRAYRGCICKQKKILEGSGKSSWLGGRQQLDGQMALSFGVRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8A1</accession>
    <name>A0A8H6F8A1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148382.1">
        <property type="nucleotide sequence ID" value="XM_037296400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8A1"/>
    <dbReference type="GeneID" id="59333896"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000036">
        <property type="entry name" value="Inositol polyphosphate phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF240">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE 5-PHOSPHATASE C9G1.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="2">
        <location>
            <begin position="640"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1027" mass="114856" checksum="6375731D89F0BBFC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8E2</accession>
    <name>A0A8H6F8E2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SGS-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005324</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SGT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148216.1">
        <property type="nucleotide sequence ID" value="XM_037296234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8E2"/>
    <dbReference type="GeneID" id="59333730"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06466">
        <property type="entry name" value="p23_CS_SGT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007699">
        <property type="entry name" value="SGS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044563">
        <property type="entry name" value="Sgt1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45862">
        <property type="entry name" value="PROTEIN SGT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05002">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51048">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="4">
        <location>
            <begin position="195"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="SGS" evidence="3">
        <location>
            <begin position="319"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="311"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="46207" checksum="E100003F55DE89C0" modified="2022-01-19" version="1">MDQAGRGASAIDGGNFKLAVTCYTEAIAQNPQAVSYYIQRSKAYTRLSPPDHQAAFHDAELAVSLATKRAKRELIADSQLRRAVTLFNLERYGDAKQCLWWVKKLNEKEKTLAIWEMKAETKLKGLEDGDERGKVTVEETPNVEVPKAVVGKKSAKEAEKTAEASASTSTTTNGTKEAAKESTAAVQSEGVQTPPSKIRHEWFQTNDTVVVSLFCKGIPKDKTTVEIKQDSLDVSFPLPTGSDFNLSLDPLFAAIDASASSYKIMSAKAEFTLKKSTPGQQWANIEGAHPASTEETSADGDDTVKRAVLSDKSSNAGPVYPTSSKSGPKNWDKVVNDLSKKPKKDVKEGEEGASEDAGIEDEEGDPTTNFFKMLYKGADPDTQKAMMKSYQESNGTALSTNWADVSKGRVETSPPDGMEAKKWDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8W0</accession>
    <name>A0A8H6F8W0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5'-3' exoribonuclease 1</fullName>
            <ecNumber evidence="5">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005472</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Multifunctional protein that exhibits several independent functions at different levels of the cellular processes. 5'-3' exonuclease component of the nonsense-mediated mRNA decay (NMD) which is a highly conserved mRNA degradation pathway, an RNA surveillance system whose role is to identify and rid cells of mRNA with premature termination codons and thus prevents accumulation of potentially harmful truncated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the 5'-3' exonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148364.1">
        <property type="nucleotide sequence ID" value="XM_037296382.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333878"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016494">
        <property type="entry name" value="5_3_exoribonuclease_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041385">
        <property type="entry name" value="SH3_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040992">
        <property type="entry name" value="XRN1_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047007">
        <property type="entry name" value="XRN1_D1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041106">
        <property type="entry name" value="XRN1_D2_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047008">
        <property type="entry name" value="XRN1_SH3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF7">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18129">
        <property type="entry name" value="SH3_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18332">
        <property type="entry name" value="XRN1_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18334">
        <property type="entry name" value="XRN1_D2_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006743">
        <property type="entry name" value="Exonuclease_Xnr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="1 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 D1" evidence="10">
        <location>
            <begin position="727"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease Xrn1 D2/D3" evidence="11">
        <location>
            <begin position="920"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 SH3-like" evidence="9">
        <location>
            <begin position="1161"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1241"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1375"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1247"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1297"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1320"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1340"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1375"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1394"/>
            <end position="1405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6218929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1431" mass="161505" checksum="55A985C8CEAC0412" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F922</accession>
    <name>A0A8H6F922_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Metal homeostatis protein bsd2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148648.1">
        <property type="nucleotide sequence ID" value="XM_037295950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F922"/>
    <dbReference type="GeneID" id="59333444"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22212">
        <property type="entry name" value="NDFIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019325">
        <property type="entry name" value="NEDD4/Bsd2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396:SF5">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10176">
        <property type="entry name" value="NEDD4_Bsd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="262"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="40488" checksum="0A50401B98446960" modified="2022-01-19" version="1">MSSQKYERVSANDEEYCQDTSQRPSNTEATPSSPPPSFRSRASSPSSRHLLSEDPITTEAERTLADTFDDGSDSDDDGHNEGDDRQRLMRASTHQSQLEQRAVHDGNGPNLSGAVTRLPVVVHPVSSNVPARPYTGATPYSTFSHSNDGVFANLNAKPERGEKLEEQPPSYEAAAADATPPYWETTILAPGHLASSDEVYVDGLPVGSLFSFVWNAMISVSFTLVGFLLTYLLHTTHAAKNGSKAGLGITLVQYGFMMRSGRSTVPPPTNGQDPAFTAPQDPNNHDFDPNAVSDSAAAAAAAAIAESSQQAAASGANSVANSDWLAYALMIVGWFILIRAVSDFFKARRHEQLVLQSPDRGLNVPVVADTERPEEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F939</accession>
    <name>A0A8H6F939_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149175.1">
        <property type="nucleotide sequence ID" value="XM_037294487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F939"/>
    <dbReference type="GeneID" id="59331976"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11608">
        <property type="entry name" value="eIF2D_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000008">
        <property type="entry name" value="eukaryotic translation initiation factor 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039757">
        <property type="entry name" value="EIF2D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039759">
        <property type="entry name" value="eIF2D_SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048248">
        <property type="entry name" value="PUA_eIF2d-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058886">
        <property type="entry name" value="SWIB_eIF2D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057429">
        <property type="entry name" value="WH_eIF2D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26292">
        <property type="entry name" value="PUA_elF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26291">
        <property type="entry name" value="SWIB_eIF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25304">
        <property type="entry name" value="WHD_eIF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="2">
        <location>
            <begin position="594"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="687" mass="75212" checksum="2786B2D47ED0DDB9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F958</accession>
    <name>A0A8H6F958_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucosamine 6-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004075</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149041.1">
        <property type="nucleotide sequence ID" value="XM_037294994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F958"/>
    <dbReference type="GeneID" id="59332484"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000048">
        <property type="entry name" value="Glucosamine 6-phosphate N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="9">
        <location>
            <begin position="40"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="184" mass="20760" checksum="7C7744EA8F8EAF19" modified="2022-01-19" version="1">MWQSPVAITGAENMSHSTHGPLFSPSLISPDVTSALPEKYTMRPLQRSDYKAGVLDVLRVLTTVGEISEQAWIERYDWMARRGDEYYILVVCDGEEKVVGTGCVVVERKFIHNLGLVGHIEDIVVNKDQQGKKLGLRIIEALDYIAEKVGCYKTILDCSETNEGFYVKCGYKRVGLEMAQYYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9A1</accession>
    <name>A0A8H6F9A1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003852</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149462.1">
        <property type="nucleotide sequence ID" value="XM_037294774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9A1"/>
    <dbReference type="GeneID" id="59332263"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034517615" evidence="2">
        <location>
            <begin position="27"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="472" mass="53315" checksum="031394FE0CA5B358" modified="2022-01-19" version="1">MFTFVQKQRQPCRLTVSLLLVTVVQALTLPINYCGDGQILSNTSTTSDHKTATNCAPNVPTINLPTNTTPNGFSSQLSLPPEPFLYRIPTTPYIIKYTNYGSPIPFHHAIQLLVTAQEEMMYEMSLLPDHVDVQFSRTRVWAEETAHPKRSGLERLAAFLCSLYQPATRYKTGGRVPTIANVWFDDIVHDAPAIARGVFQFLYALSSALFQVQQSILYNSLVAVAEDEQMKALQDCSVSCTTAFNTAHTKVDSSHLAASDQERSVEYVKDAKVGFTSKKSHEMQSEQLGPEKDGQNIISRNGQSLERLKKERIMNKQADHTDHHYQKQRDLKASEANGILRSIPPPAIYWAVDPNGETLYFHNPTALMDAYGTDVVEKIYDDIKFFLSFHPPNLPDPLRHFNHTSDSRPSAATTEGFLKQLLRNDCDHITRVIALLFGIASPEMRNEYRLVVENIADYRRMDTIEEELIYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9M6</accession>
    <name>A0A8H6F9M6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149521.1">
        <property type="nucleotide sequence ID" value="XM_037294140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9M6"/>
    <dbReference type="GeneID" id="59331628"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="109" mass="12203" checksum="BC590AA9C8D8FE80" modified="2022-01-19" version="1">MATIERSRYVTIPGETAPNNKIDWEKGIQSLKISYEAHTTLLRNAYRRQDEASADLSAHKAGLIVLQEKLDLLHGEGAGTPIKLKEQQNIFEAIEPNAEFNKNEAGDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9P6</accession>
    <name>A0A8H6F9P6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149897.1">
        <property type="nucleotide sequence ID" value="XM_037293819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9P6"/>
    <dbReference type="GeneID" id="59331306"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="244" mass="27718" checksum="6729D32C3DED0722" modified="2022-01-19" version="1">MAPAATTRSKNAAKRVTPASKVIKKTSKAAKKAPKAKKATKAKTPPPREEIVVYKTSSSTISPDEIEETPPSTVKVDLQELIAQLSKVQEERNHRHRHEGRVYKSRRHHHRHRRSHRHRHSNTSDSNSSDSESDWEERRGVPFRHAEDKKPSLSIAETFRSVDVEYFKQIFFGTFKTKNFAKLAHMHTTPAKEDKDVNGHNHMQTESRISLSVTPPSSTQGPPSGTRRQDDSSPTQNEYDTKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9Q1</accession>
    <name>A0A8H6F9Q1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002909</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149912.1">
        <property type="nucleotide sequence ID" value="XM_037293834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9Q1"/>
    <dbReference type="GeneID" id="59331321"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="429" mass="48711" checksum="F7C3BC9DA4934718" modified="2022-01-19" version="1">MADSRELCPFLSLPLEIRNPIYSYLLSTKYTREDMIQKITISDSKYKGSHYRPAHKYHFQPAILRCNHQVNQEATAVFHNTNLFVSVSHNYSSGEISAFGEKFDLLESQCDGRGFPVLARGKKARLFDGYAMEIYLGRANRRDARGRWWNSPKPQKWEIRPKFMIASDDLLQFCKIFLLLSMRAAKTDAESDSMLHLKKAMLLVDVLHKTDQHNRANEGPRPAIRRLLEPLAYFHNIGLVIIEGPVPTLYSEAIRQRIQRRAPSAQDLVHAVSSLVEQGDAAFLKRDFHSAVFVYKSALEKLHAGSQKRPKLERMVEGTYTGMVIGGVKNLLQHKVYLGLSKASLQLKRFEMAHHWTLAVARGLRDSREILSQMWFCRALASKGLGELEQALKEMEMARLIQPNDLGILAEIAILSSLLGPGKQQAEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA33</accession>
    <name>A0A8H6FA33_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149669.1">
        <property type="nucleotide sequence ID" value="XM_037294288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA33"/>
    <dbReference type="GeneID" id="59331777"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="189" mass="21388" checksum="BE300251B3C0130E" modified="2022-01-19" version="1">MNSYAARLASFTSWPHVCPSASTVARAGFRREYDPTFPSPDLTRKKHRNQKTRQQYTRRSLQITQPATQSITQPATQPAHQSTQLATQPEHSVKQEEELQQANAENTAEKASGSKAISTKNTAHFSMIPRQVATQQAENDTAIWPIIPTVSVWYITAMSTQEKDLLRRSQPIGPLQARITYRTMHIWTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAN8</accession>
    <name>A0A8H6FAN8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial 2-oxodicarboxylate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_002588</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150343.1">
        <property type="nucleotide sequence ID" value="XM_037293514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAN8"/>
    <dbReference type="GeneID" id="59331001"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005310">
        <property type="term" value="F:dicarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000034">
        <property type="entry name" value="Mitochondrial 2-oxodicarboxylate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="107"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="202"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6220908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="32749" checksum="B9255746107B27E0" modified="2022-01-19" version="1">MSQDKPLPFIYQFAAGAVAGVSEILVMYPLDVVKTRVQLQQGKGVGGEGYNGMIDCFQKIIRNEGFSRLYRGITAPILMEAPKRATKFAANDAWGKYYRGIFGMSKMNQQLSILTGASAGATEAFVVVPFELVKIRLQDRASAGKYNGMIDCVAKIVKEEGPLAMYNGLESTLWRHMLWNSGYFGVIFQVKGWLPKAEGKGQQIRNDLVAGAAGGTIGTVLNTPMDVVKSRIQNSPKIKGSVPKYNWAWPALGTVMKEEGFAALYKGFLPKVLRLGPGGGILLVVFTGVMDFFKKMRGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAQ2</accession>
    <name>A0A8H6FAQ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Srp40 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150977.1">
        <property type="nucleotide sequence ID" value="XM_037293324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAQ2"/>
    <dbReference type="GeneID" id="59330811"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039191">
        <property type="entry name" value="Nopp140-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007718">
        <property type="entry name" value="Srp40_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216:SF1">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05022">
        <property type="entry name" value="SRP40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Srp40 C-terminal" evidence="2">
        <location>
            <begin position="528"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="401"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="441"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="473"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="597" mass="63119" checksum="0EB740564331BB8C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAT5</accession>
    <name>A0A8H6FAT5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_002126</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150706.1">
        <property type="nucleotide sequence ID" value="XM_037293053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAT5"/>
    <dbReference type="GeneID" id="59330540"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000051">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002975">
        <property type="entry name" value="Fungi_Gprotein_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF302">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-5 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01241">
        <property type="entry name" value="GPROTEINAFNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="150"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="175"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="269"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="41087" checksum="A302BCE245A938BE" modified="2022-01-19" version="1">MGCGMSTEEKEGKERNEQIENQLKRDKLSQRNEIKMLLLGAGESGKSTILKQMKLIHEGSYSRDERESFKEIIYSNTVQSMRVILEAMESLELPLDDQRTEYHVQTIFMQPAQIEGESLPPEVGSAIDALWKDAGVQECFKRSREYQLNDSARYYFDSIGRIAQPDYLPNDQDVLRSRVKTTGITETTFIIGELTYRMFDVGGQRSERKKWIHCFENVTTILFLVAISEYDQLLFEDETVNRMQEALTLFDSICNSRWFVKTSIILFLNKIDRFKEKLPVSPMKNYFPDYEGGPDYAAACDYILNRFVSLNQHETKQIYTHFTCATDTTQIRFVMAAVNDIIIQENLRDCGLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAY3</accession>
    <name>A0A8H6FAY3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150856.1">
        <property type="nucleotide sequence ID" value="XM_037293203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAY3"/>
    <dbReference type="GeneID" id="59330690"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="311" mass="34971" checksum="CEC3D2AAF81F7FFD" modified="2022-01-19" version="1">MADNQADNPEPREGTSNSEVVSVRSRASSVISTGTKFSISTLPQGHFQIDDVLEGNAARTTSTRPGSMYSIRSIAPSLPPYEDQAPRPAPIQTSAPSGNDSEQPPTPSTADPENALSIHYGRVVRTIDENHARQLARIYEAHDQQLKTHVQELAATREAVDQAYRKEWKANNREIERIREDAAASVAEMQKTLDRDLERGREGCAAKVAALEERIQSLSITHTKSLARIQQDATDQVIMLHEEYQATIDKARNAIEDLWEGRWNDRTRLAAEEAQRRDLERDAEWLRLIENTHPELLDEMRTAMEASRAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBG3</accession>
    <name>A0A8H6FBG3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Subtilisin-like serine protease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151680.1">
        <property type="nucleotide sequence ID" value="XM_037292261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBG3"/>
    <dbReference type="GeneID" id="59329747"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046536">
        <property type="entry name" value="DUF6601"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34414">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34414:SF1">
        <property type="entry name" value="SUBTILISIN-LIKE SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20246">
        <property type="entry name" value="DUF6601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="37535" checksum="D9A06AF1EC2AB324" modified="2022-01-19" version="1">MSPPFAEEHQLDDMHPPYPQYDDRLPSQPLISITSEPGQLFDFLDKDLLTLDLDRMAPKLWLMATQSGSHISPLHHQRIKGRKIVITEDPRLHLVWIEDRIFVKPLPLYLQSYTFWAKYLYHSTTEPNEKAPDTMFPDWSIRKEPISRAALGLLRSYAFLIRHSSDLDIAIHHRLLPPDKTFLSICLFTSQLLSIPDSEVSPRYGYGELRLTRLNFWSKIFLNRWNYETVHRQYSQYFQRFYAPLLFAFGFFSVVLSALQVEMAVESVWTSHQWQAFWLFSRVFSIVSLAVVATPALGLLLLFLGKNMMELEYALTHRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBN8</accession>
    <name>A0A8H6FBN8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151349.1">
        <property type="nucleotide sequence ID" value="XM_037292810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBN8"/>
    <dbReference type="GeneID" id="59330296"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="23692" checksum="277763AD36199C2A" modified="2022-01-19" version="1">MSFVWSDPFKPQAEVRSPKVPSISTVLKDTKAQPQPIYSRGLIVYDKSDVDSRGEPTRKKLWIWEMKYDPTYESASSHRERKKALSYSVKDGPGEKAKDMGLVSEEDLRETEPEPPTLFEPGAIVFDKRDRDSEKKSTHEKLWVWEVTYHPPPYPRVKSHLLRDGPGGQAKEMGSENGHREGGKEFDWLSIKGEINPRTHQREGGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCC4</accession>
    <name>A0A8H6FCC4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151965.1">
        <property type="nucleotide sequence ID" value="XM_037291603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCC4"/>
    <dbReference type="GeneID" id="59329084"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="660" mass="71192" checksum="A202B1BB89F3A54F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCM5</accession>
    <name>A0A8H6FCM5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RAVE subunit 2/Rogdi</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152325.1">
        <property type="nucleotide sequence ID" value="XM_037291963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCM5"/>
    <dbReference type="GeneID" id="59329448"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028241">
        <property type="entry name" value="RAVE2/Rogdi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618">
        <property type="entry name" value="LEUCINE ZIPPER CONTAINING TRANSCRIPTION FACTOR LZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618:SF1">
        <property type="entry name" value="PROTEIN ROGDI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10259">
        <property type="entry name" value="Rogdi_lz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="32373" checksum="15FB53E24B6016B7" modified="2022-01-19" version="1">MSATVWPPISHEQLIKEEEKSLACELEWLLNLLQESLASLKSGLQECVALLAPKEPGSTLALSSLRSESVKGFVTRIGTRIIKGDINLRLTTLPPPRSLSSYPVHLQYPVPLPQLTALLSLLNQALDIIDISRWTGDPHNGAFISGQLRLLADTIEEAQQTLKGGEDVVGGKWWEDALTEDTSSPSVPPSLSLHLSIADAALLLHVRTLAPPSTPESSLTGLSLRTRLGLGPRPPVHDELDQVFHYRGEEVIVKEKVRVESQDPSLMAVLAKLSALGHAVGGWRLKVGVVMGEDLEGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCM7</accession>
    <name>A0A8H6FCM7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000288</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152662.1">
        <property type="nucleotide sequence ID" value="XM_037291227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCM7"/>
    <dbReference type="GeneID" id="59328707"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015215">
        <property type="term" value="F:nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000102">
        <property type="entry name" value="Folate carrier protein Flx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="9"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="109"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="212"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6223445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="33897" checksum="55F8A5A70895432E" modified="2022-01-19" version="1">MSKEYRQLPRGLIEAFAGFAAGIASTLVAHPLDVIKTRLQVDRDSPSQFGGSLRLVKRIVRNEGTISALYRGLTPNLVGNSLSWALYFVWYDRTKEGIRSYRGFENGGLSYYDFFIASGTAGVLTAVATNPIWVIKTRMLSTSSSHPGAYKSISDGTRQIYRSEGLLGFYRGLVPSLVGVSHGALQFMAYEQLKIYRGHSLDGGQKDLGAADFLVLSGLAKMFAGSATYPYQVVRARLQMYDADRTYKSARDVVAQVWRKEGLAGFYKGLGPNLLRVMPSTWVTFLVYEKTKVLLPRMLDQRSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCP3</accession>
    <name>A0A8H6FCP3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">37S ribosomal protein S9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000333</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152707.1">
        <property type="nucleotide sequence ID" value="XM_037291272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCP3"/>
    <dbReference type="GeneID" id="59328752"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000001">
        <property type="entry name" value="30S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_uS9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001099">
        <property type="entry name" value="PRK00132.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US9M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="297" mass="32897" checksum="09FD774F423D603B" modified="2022-01-19" version="1">MASKLSWPLSSRWTIQCPKCHGEILRKRTFISTGSSRAIAAPPVNLTQNSPSNPQNARVVPASPSYFTGRPDSTDNYLNLLALLRRYQTLPTVAPAQAPRVAWRTVTQYRLLVGEPIRASKYHKVVEVLQRLNRIHPAVMPAPLKEAMEIYKRDIDPYAVTKRPNIIDADGRAYGVGRRKSSSAKVYLVQGEGEVLVNGKSINSAFGRIHDRESALWALKATGRMDKYNVWALVSGGGSTGQAEAITLGAAKALMVFEPALKPALRRAGCVTRDPRRVERKKPGHVKARKMPAWVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCR3</accession>
    <name>A0A8H6FCR3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152483.1">
        <property type="nucleotide sequence ID" value="XM_037291048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCR3"/>
    <dbReference type="GeneID" id="59328527"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="285" mass="32340" checksum="527B36B0CD1219C3" modified="2022-01-19" version="1">MMNPLSCNILDENFIQARKESATIPTSTFPFLSLPQELRDRVYDSHLSATYNHCLPDSSKLWVFPFIRKTPHFQGLQERHFGGLAILLVSKSVHEEAKLILYKCGTFFFNVPQAIAALRHSLLSTADLQNIIIDLDFDIIFSSLHSQDATDLVKHFAQQNSTLTCRSCEVQFRFVSETDFLLGEMGEVKALKDAIDSVKRESESLSNEKSLAFARNEYVKFNRCWHVVENLDLGAGEGNTTDRDSEESLLLEKEINIFHLPALKFQAYAIVSVFAPDWTATHQTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCW7</accession>
    <name>A0A8H6FCW7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152466.1">
        <property type="nucleotide sequence ID" value="XM_037291031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCW7"/>
    <dbReference type="GeneID" id="59328510"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="65960" checksum="33741F26810F74E8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD15</accession>
    <name>A0A8H6FD15_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ustYa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152601.1">
        <property type="nucleotide sequence ID" value="XM_037291166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD15"/>
    <dbReference type="GeneID" id="59328645"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021765">
        <property type="entry name" value="UstYa-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33365:SF6">
        <property type="entry name" value="OXIDASE USTYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33365">
        <property type="entry name" value="YALI0B05434P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11807">
        <property type="entry name" value="UstYa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00035112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="213" mass="24701" checksum="25AAFB507FB51C7D" modified="2022-01-19" version="1">MAVSYHDRSRYANLEVDTTLTWQEVTEYSSDNMTLTDERWKALSADDGIVAVDREWAAREGLPPSENVFPWDSSKRIYLLNGYHGIHCLAQLHLTLREYRDGLPQSHSFEHSNHCLDWLRGDIMCRADDTPLYTTRSMVSANGIGQSRQCRDWGKLESWARTHHSCYRYGDFVVEDKLPSQMGRFKYCPNDSPYLPAVRKYFGKGDDWLPVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDK6</accession>
    <name>A0A8H6FDK6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153226.1">
        <property type="nucleotide sequence ID" value="XM_037301592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDK6"/>
    <dbReference type="GeneID" id="59339130"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="638" mass="69946" checksum="7C3F18AAB359FF0C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDU8</accession>
    <name>A0A8H6FDU8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_011013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153496.1">
        <property type="nucleotide sequence ID" value="XM_037301862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDU8"/>
    <dbReference type="GeneID" id="59339403"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="110" mass="11831" checksum="547A9DFE3BE8C380" modified="2022-01-19" version="1">MATPTMAAPTMATPTILPWLPTMATTTMAATTMAATTMATTTIATYHGYLPWLPTMAAPTMAAPTMATPTMATYYGYLPWLPLPWLPLPYLPSKSTTTIATTIMATTIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE42</accession>
    <name>A0A8H6FE42_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009914</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153588.1">
        <property type="nucleotide sequence ID" value="XM_037300771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE42"/>
    <dbReference type="GeneID" id="59338307"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000023">
        <property type="entry name" value="Sensor histidine kinase/response regulator, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF72">
        <property type="entry name" value="HISTIDINE KINASE_RESPONSE REGULATOR, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G06140)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="474"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="973"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="171"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="251"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="951"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="171"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="268"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="1025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1121" mass="124546" checksum="529461CE5B6C7C10" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEB9</accession>
    <name>A0A8H6FEB9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">ER membrane protein complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_010125</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153798.1">
        <property type="nucleotide sequence ID" value="XM_037300981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEB9"/>
    <dbReference type="GeneID" id="59338517"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009445">
        <property type="entry name" value="TMEM85/Emc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19315">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06417">
        <property type="entry name" value="EMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017207">
        <property type="entry name" value="UCP017207_TM-p85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6224931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="190" mass="21405" checksum="B4941940FB2030A7" modified="2022-01-19" version="1">MAQESQPKHLPPPPPKWVINLNSPPRRKANNTHVTNPPGYTEASSGKRNAASSKTPARKPPSTAETDTLKLKKSWELALAPAKALPMNAIMMYMSGNSLQIFSIMMVFMLFKNPITGIFTTNEVFRRFESEGTRKQLWVVKACYVLMNCVALALGVWKVNGMGLLPTTRSDWLAWETERQPLERVIFAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEV6</accession>
    <name>A0A8H6FEV6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum chaperone BiP</fullName>
            <ecNumber evidence="4">3.6.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Immunoglobulin heavy chain-binding protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_010471</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154141.1">
        <property type="nucleotide sequence ID" value="XM_037301324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEV6"/>
    <dbReference type="GeneID" id="59338862"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10241">
        <property type="entry name" value="ASKHA_NBD_HSP70_BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000009">
        <property type="entry name" value="DnaK-type molecular chaperone BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000153">
        <property type="entry name" value="Endoplasmic reticulum chaperone BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042050">
        <property type="entry name" value="BIP_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0346">Stress response</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034229276" description="Endoplasmic reticulum chaperone BiP" evidence="15">
        <location>
            <begin position="37"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="661"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="672"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6225274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="684" mass="74752" checksum="C85BB25276DFE275" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEZ0</accession>
    <name>A0A8H6FEZ0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009877</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154584.1">
        <property type="nucleotide sequence ID" value="XM_037300736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEZ0"/>
    <dbReference type="GeneID" id="59338272"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="119" mass="12606" checksum="FCD2B8A0A7A18D3B" modified="2022-01-19" version="1">MGAIEPAAANRSLSTIRTELEFLRESGLLNPMQFQSIMSQLPQPGGIPSSYIDPRYAQGANNYVNMPQLAQAAQDPNHPANPQHPKHGAWAKKLGEKLGNAAIFGAGATAGSDLVNSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF94</accession>
    <name>A0A8H6FF94_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154728.1">
        <property type="nucleotide sequence ID" value="XM_037299902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF94"/>
    <dbReference type="GeneID" id="59337436"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="131" mass="14865" checksum="873DA4FE052D13DB" modified="2022-01-19" version="1">MCYYNIFYFECGCESQSPAFQFPCRPARAGQRSVYHDFDHHHGPPQACPLFIVHRERITPGKCASHSLQAQLEKRIKALKDEIKRLKDDIAEHQDLIDQEKKVRRARNHGRPGYGGPGGHGEHDKCGGGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFA7</accession>
    <name>A0A8H6FFA7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isovaleryl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009755</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154462.1">
        <property type="nucleotide sequence ID" value="XM_037300614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFA7"/>
    <dbReference type="GeneID" id="59338150"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:3-methylbutanoyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000021">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase IvdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000003">
        <property type="entry name" value="isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000004">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF18">
        <property type="entry name" value="ISOVALERYL-COENZYME A DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="8">
        <location>
            <begin position="51"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="7">
        <location>
            <begin position="165"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="6">
        <location>
            <begin position="272"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="47175" checksum="6BDB35A170C0B7AA" modified="2022-01-19" version="1">MSTPYIARTITSTIRQVGRSSPRPRPIHVTVQRPASTKHPRGFVPPTVDDLAELRERVQEFTRREIPEKVAARTDRDNDFPSDIWRKFGEAGFLGITADEDYGGLAMGYQAHCIVMEEISKASGSIGLSYAAHSQLCINQLSLNGNNDQKQRFLPGLISGQKVGALAMSENSAGSDVISMKTTARAVNGGWVLNGSKMWITNGPDADHIVVYAKTEPDKGSKGITAFILDSTAQGFSCARKLDKLGMRGSNTGELVFEDVFVPQENMLGQVNHGVRVLMEGLDIERLVLSAGPLGLMSSALALVLPYTHSRIQFSTPIAHNQLIQAKLADMYTKYRASSSYTYSVANALDAAHSTPNNTPNTKDCAGAILYAAERATECALDAIQLMGGMGYVNEVPAGRLLRDAKLYEIGAGTSEIRRIVIGRAFNKEYDESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFD4</accession>
    <name>A0A8H6FFD4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154848.1">
        <property type="nucleotide sequence ID" value="XM_037300022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFD4"/>
    <dbReference type="GeneID" id="59337556"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="253" mass="27786" checksum="5995F4B2D2582B74" modified="2022-01-19" version="1">MGKRLAEDFILMSHLADKCGLQEETSRSFASSSVTRSHVTQELGLSANRDRADLPLALVALISQSLPWTVKLISGGEPHSMHHALFVYCRSRSVLHVHTSATIQQKLNACHENTSEKPGSNPTDVAKTRADAGQPSRIDEKAVDPEDRSDRAGAASPDPEPHPSRTMTVRRILGMYDGLLTRPRDAGGRRGRFRRVVVESTRAVVRCVTSSSGNARPSRRDGSTPPAPARTFVFSRSEEWVREAPVLAWRSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH41</accession>
    <name>A0A8H6FH41_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2',3'-cyclic-nucleotide 3'-phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156286.1">
        <property type="nucleotide sequence ID" value="XM_037298950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH41"/>
    <dbReference type="GeneID" id="59336479"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009187">
        <property type="term" value="P:cyclic nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012386">
        <property type="entry name" value="Cyclic-nucl_3Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141:SF1">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07823">
        <property type="entry name" value="CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22685" checksum="895E4C03FEAD5C4E" modified="2022-01-19" version="1">MPGCSLWLLPPSDSPIQLVLSDLVTTKIPSLFPELHNVVFEPHMTLTSEITLPPDISGNKRAQRWLDDLPLPKKVNVDVCLESLDVGAQYFKKLILSVSKGPLETFGAHVRSTAVENGGMTAATSWVKETWAPHVSLMYADIEVTAEKRQEILQVVAEAGIRIEKQNVLLEDGKGDYSGWTGGRIALVETWKELRDWEVVAARAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHJ0</accession>
    <name>A0A8H6FHJ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008096</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156300.1">
        <property type="nucleotide sequence ID" value="XM_037298964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHJ0"/>
    <dbReference type="GeneID" id="59336493"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12398">
        <property type="entry name" value="RRM_CSTF2_RNA15_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.70">
        <property type="entry name" value="Transcription termination and cleavage factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026896">
        <property type="entry name" value="CSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038192">
        <property type="entry name" value="CSTF_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14304">
        <property type="entry name" value="CSTF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="205"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="207"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6228366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="308" mass="33292" checksum="375EEA006EF22683" modified="2022-01-19" version="1">MADKAGRVVFIGNIPYGLTEEQIIHIFGQVGQVLNFRLVYDNDTGKPKGFGFAEFADTDAAASAVRNLNNHEIMGRELRVDFSHVGGKDDGAPSGYNAQHPPQNPPPNGYQPPVPGGLPPLPPGIDLQPNITCPDAISKTLSTLPAPQLLDILSQMKGLVMGDPAKATELLKQAPQLAYAIFQALLLMGLVDTSVLGSVVEQASHPVQPPAHPVPPRQAPNYQPPPVQQPYPQYPPISGQMHVPTPPVHNQLYQPPPAPPPPLPPQQQPANQEELLQQILNMPQQQIDQLAPADRAQIMAVRQQLRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHP1</accession>
    <name>A0A8H6FHP1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008262</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156465.1">
        <property type="nucleotide sequence ID" value="XM_037299129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHP1"/>
    <dbReference type="GeneID" id="59336659"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000386">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF39">
        <property type="entry name" value="TRANSPORTER LIZ1_SEO1, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G00980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="484"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6228531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="57525" checksum="68D059D479820695" modified="2022-01-19" version="1">MHLNEDQDRREPCDKADLIIQENVQRKWQSYLWDTLDKSPEERTFLFKLDFALLTFASLGYFIKYLDQANINNAFVSGMKEQLGLYGNELNYMQSCWTAGYVIGQIPSNILLTRIRPSIWIPSMEIAWTVLTFCLCRCTNARQIYAVRFFVGLAESSFYPGMQYVIGSWYRKDELAKRSCIFHVSSAIATMCSGYLMAAVYHLGGRGGFAGWQWLFMVDGIISLPIALAGYLFIPDVPETSRAFYLSKNDRAFAKKRMQLEGRKPRAPYTKAKIRKILTSWHIYALTALYVFFNNGGAGAVPVFAQFLKDSKHPKYSIAQINDYPTTTYAVQIITTLLYAWTSDSILSGDRLPPIVFGGAVNIICYISLAIWAIPEGWRWTCYILSGAGFGLSGLCMAWAHEICADDNEERAIVVASMNEMAYVLQAWLPLLVWQQIDAPQYRKGFITVTVLSASLIVTGFITKALHKNQIRRQSYNVQARDHQVDGETVRSSGEDVTSENVQMAAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI26</accession>
    <name>A0A8H6FI26_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156567.1">
        <property type="nucleotide sequence ID" value="XM_037297932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI26"/>
    <dbReference type="GeneID" id="59335437"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="390" mass="43622" checksum="55FADAC405C313A9" modified="2022-01-19" version="1">MSGLYSIQGCGTNASRIATILSSLSPFLQPAITDSEKTFTSGAYNTFFKDVHNTPFVRKILQNIAFGTPVLPGLAQSGFGRVTSPVIACIPDPPIVNPDPKLVGLTPWVQAFAKQDCHDRGDRVYGIFVGGTNIVALCPGWFIFNRPDEPKEKRPCIAVDRATNRFRGLGNPLAQNSRAWLLHELAHVYLVAATNKRVPYIEVYSINDCFRLNAGSQKYMPNNYVYYAGSVFYTCTNFPTVSFRDRELSTSSSNATDDVVDYDREERREIQGDKSEARDTAFKLLYLILLRDFKDEMQDSATVFNAVLYLVSCGDFQMEDEERSSGCVRGAIRNLNKADSRVGQVEKDTAELTVEDAGDVTTEEEKSDCYYTSDHSDYIAKRSWMIGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIB6</accession>
    <name>A0A8H6FIB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007183</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156711.1">
        <property type="nucleotide sequence ID" value="XM_037298076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIB6"/>
    <dbReference type="GeneID" id="59335583"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17871">
        <property type="entry name" value="GPN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000338">
        <property type="entry name" value="GPN-loop GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030231">
        <property type="entry name" value="Gpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF3">
        <property type="entry name" value="GPN-LOOP GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="295"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="345" mass="38553" checksum="0CE803945096DB36" modified="2022-01-19" version="1">MPFAQLIIGPPGSGKSTYCDGMQQFLSAIGRKCSVVNLDPANDRTSYPCALDVRDLVTLEEVMAEEELGPNGGVLYALEELEHNGDWLDEGLRGLGEDYVLFDCPGQVELFTHHSSLRNLFFRIQKLGYRLVALHLTDSYCLTLPSLYISTLLLSLRAMLQMDLPHLNVMTKIDKLASYGPLPFNLDFYTEVQDLSYLLPHLANENPVMRHPKFEGLNNAVVELVEDFGLVGFETLAVEDKKSMMTLLQAIDRAGGYAFGGAEGANDTVWQVAMREGMTGMTIHDVQERWIDRKEEFDELEAKEREEEARAQETPPLTEDGGEEDSDVEEMGPVPDSGVKVLRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIG7</accession>
    <name>A0A8H6FIG7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_007235</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156763.1">
        <property type="nucleotide sequence ID" value="XM_037298128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIG7"/>
    <dbReference type="GeneID" id="59335635"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000040">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="114"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6229121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="320" mass="36426" checksum="56E1D085E8B2ACA0" modified="2022-01-19" version="1">MPGLPASVDLDECIERLYKKELLADSVIEAICAKTKELLMKESNVVHIAAPVTVVGDIHGQFFDMIEIFKIGGFCPDTNYLFLGDYVDRGLFSVETISLLVCLKLRYPSRVHLIRGNHESRGVTQSYGFYTECNRKYGNANVWHYFTDMFDFLTLSVVINNQIFCVHGGLSPSIHSIDQIKIIDRFREIPHEGPMADLVWSDPDPERDEFSLSPRGAGYTFGAQVVKKFLDVNKMNHILRAHQLCQEGYQVLYEDRLSTVWSAPNYCYRCGNMASVLEVSDQGERYFNVFDAAPENDLHRGEQQNQPNQDGQGVVVEYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ21</accession>
    <name>A0A8H6FJ21_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007547</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157073.1">
        <property type="nucleotide sequence ID" value="XM_037298438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ21"/>
    <dbReference type="GeneID" id="59335946"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="6">
        <location>
            <begin position="271"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="446" mass="50252" checksum="B5A557CB8F1C4F6D" modified="2022-01-19" version="1">MKSLAEVAEFYEHLTHHIVPGPNSYSYFVPFLLLPLALLTPPSVLSRSQLAFLFLPAIFACQIHSWYAAGIDVISVDLALWSFVLLVCRDPRKTHRRIWISTPSDSGENRPSDTVEDIVVEEAYPEKLAKRIPWVFTLLVSLRLTGWKIGDPSHDKTQPPARLSRSAFLRHAVKTVVQSYLILDAAAFYAWTDPYFTTSGMEVGQPFPPPNAQMATWLVTLRLLPPRFLRSCVLAGQIYAMVTGMFYVPLIPMVGLNAVGILPYEWSPHTWPLPFGRFSAIPERGLRGLWGDWWHGVNRQFTAPPGRWLAQALGIPTKSITGFALLMISAFFFSGVMHMGLIPPEPETSLLSARWMRLHIAGFFWAQIPAFGLELAVSKLVARFIPQALDWSVTKALVIAWTAAWLCLTLPLLAVPFRELGYWHYYPVPVSLLQGLSGKGLWTWTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJS5</accession>
    <name>A0A8H6FJS5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA sliding clamp PCNA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004208</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="7">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processivity during elongation of the leading strand.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157128.1">
        <property type="nucleotide sequence ID" value="XM_037295127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJS5"/>
    <dbReference type="GeneID" id="59332617"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000006">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000008">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000001">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.150.10">
        <property type="entry name" value="DNA Polymerase III, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00293">
        <property type="entry name" value="PCNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="9">
        <location>
            <begin position="21"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="10">
        <location>
            <begin position="147"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6229871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="30579" checksum="71AB60B59658D6FB" modified="2022-01-19" version="1">MSMTVGECLLCGLQEPYKAVLLEARLEQANLLKKVVDAIKDLVQDCNFDCNDSGVALQAMDNSHVALVSMMLKAESFSPFRCDRNIALGINLTSLMKVLRCAQNEDILTLKAEDAPDVVNLVFESSESDRLSEYDIKLMDIDQEHLGIPETEYAATITMPSAEFQRICRDLMALSESVAIEATKDGVKFSCSGDIGNGAVTLRSHTNVDKPDLNVEIALSEPVALTFSLKYLVNFCKASGLSSTVKLCLSNEVPLLVEYGLASNSYLRFYLAPKIGDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKJ1</accession>
    <name>A0A8H6FKJ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004564</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157481.1">
        <property type="nucleotide sequence ID" value="XM_037295480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKJ1"/>
    <dbReference type="GeneID" id="59332971"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000025">
        <property type="entry name" value="FIZZY-related 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF1">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="10">
        <location>
            <begin position="262"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="306"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="389"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="431"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="519"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="140"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="199"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="205"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6230224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="577" mass="63863" checksum="28B34D35419E947F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYM4</accession>
    <name>A0A8H6KYM4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aspartate kinase FUB3</fullName>
            <ecNumber evidence="3">2.7.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Fusaric acid biosynthesis protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_007317</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156843.1">
        <property type="nucleotide sequence ID" value="XM_037298208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYM4"/>
    <dbReference type="GeneID" id="59335716"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2130.10:FF:000001">
        <property type="entry name" value="Bifunctional aspartokinase/homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.10">
        <property type="entry name" value="VC0802-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054352">
        <property type="entry name" value="ACT_Aspartokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499">
        <property type="entry name" value="ASPARTATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499:SF59">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22468">
        <property type="entry name" value="ACT_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="10">
        <location>
            <begin position="162"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6229201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="240" mass="25894" checksum="3E390A7B9B497285" modified="2022-01-19" version="1">MEHIQKAKIPLRLKNVKDPSGAGTIIFPSNHLASDVSAEPNEIPSAARSGISTPTSSFLLANGYHGDMQQRRRPTAVTVKDSILIINVLCHRNNKSHGFLAEVFRRLDQHKVVVDLITTTEKSISLAVSAATEGAREIKNATSEIEKLGTVSVTTGMSIISVVGHKMRNTVGIAGEIFSTLAGAKINIYLISQGASEINISFVVKESDALLAMNAVHTKVLQIPAHSEQENSFSKGPWLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYU6</accession>
    <name>A0A8H6KYU6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156977.1">
        <property type="nucleotide sequence ID" value="XM_037298342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYU6"/>
    <dbReference type="GeneID" id="59335850"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="263" mass="28849" checksum="7917E2B7E82710FF" modified="2022-01-19" version="1">MSYELTKPDKRTTPQLGSPFLAESVAGPRDDSSTSYQTLIWGNRPKIQIIAGQHRKLALKIHEPTNFTWLANVYDLDAMPPTTLIELGGNATTVAHAASMGEVFRDIAAIAPHIASSETVIVRPYLTPQCAPTLEEYLGACRHGDKAKLQKTFMNALTVCSQQKSIDKLIIVLASPLRGELSRFVNSGAGIKLFSQDGMRDMFRKPGKYRESVLNAHGSRDKRVGISRMASVNGHAHHDLGSRLPQDLRQSRTGSESEVSVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L000</accession>
    <name>A0A8H6L000_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157703.1">
        <property type="nucleotide sequence ID" value="XM_037295702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L000"/>
    <dbReference type="GeneID" id="59333195"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="480" mass="53415" checksum="4B722107A9465FFC" modified="2022-01-19" version="1">MSPTDKVDSWFASTPAGPSQSSTSRPNTRDTALTSLAGDQSQLSNQNTGDAFFDPTATGQRTRDLFLDSFVDDPQLPTAQRDASTRIPGSLPTNQSKPSTSRLSTRDPFFDSVPKGPSQVSTSRWNARAPAFNVWNSARKQAPASPWSKTEPSVSPVERVQENSRPRLTLRIPDTSIGSDNFRSAPQMATHSEIPDLQADARRTTDSLVPAARPSNPGLQGSQSPASWRGEMLNPAARPFQSKFSGPQAPASRNNHTVVQPTEASHTNSRVWSSKLIVEKERWKRVEESLGRMNLLRSSVVPKTLKDWLAHQRGRADAKKTREQQKLKALVQSPRDPRLPPPSRLVKIGPAFGGKSFEHKNFCAILVFPSVWVRTYEPPAERPDPLWPCVEEMKEEGDERNTSGFRRFPGLPRVPGNDTVQYKLKAIQPFLPFDAIWELPTKESIEAANEKYSREETEKMEGYLGRSLLDALDCITEDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C637</accession>
    <name>A0A8H6C637_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_006627</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147235.1">
        <property type="nucleotide sequence ID" value="XM_037297525.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59335028"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF15">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE APDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="614"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="10">
        <location>
            <begin position="970"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="9">
        <location>
            <begin position="1104"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="1212"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="80"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="236"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="471"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="792"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="344"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="803"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6217800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1389" mass="152332" checksum="C81C93E897E010F0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6S8</accession>
    <name>A0A8H6C6S8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_006374</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147397.1">
        <property type="nucleotide sequence ID" value="XM_037297272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6S8"/>
    <dbReference type="GeneID" id="59334775"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF39">
        <property type="entry name" value="MONOSACCHARIDE TRANSPORTER, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="445"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="52"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6217962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="550" mass="60250" checksum="3082E44F0579EB21" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C701</accession>
    <name>A0A8H6C701_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147552.1">
        <property type="nucleotide sequence ID" value="XM_037296978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C701"/>
    <dbReference type="GeneID" id="59334480"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034534278" evidence="1">
        <location>
            <begin position="24"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="175" mass="18230" checksum="6D2CF906951E0E2F" modified="2022-01-19" version="1">MRSLCLLQTAILHISATITSISAATIPHPTPHLAPLNNATAPPTLAVWPTLPWWTKLRNRNLDPPDIVSVADHAATPQQTYILQELVLLPLARRIHGGAGPANGSEAISEGGVGAVFVPVRGRAPGREVAATAVAALAMAEWSFGARGLRAGVRVGEVRVAGLEVWVGGEVGRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7S8</accession>
    <name>A0A8H6C7S8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147983.1">
        <property type="nucleotide sequence ID" value="XM_037296802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7S8"/>
    <dbReference type="GeneID" id="59334303"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="202" mass="22688" checksum="3E59A7EE766224AD" modified="2022-01-19" version="1">MTRRLVYGGALIAFLAAFALTLASIIIPRWISWDSETPSGAKIHYTYGLHRRCSSLTNACEYFPHEEDCHGDRYFCSMWRSVGFLTSFTVVLEGMTLITYIVILVGGKQKRESGWKILAGLHLLCGAVECAGMAIVAYLYENDDRFFPGWKLDISWIFLTISWSIMMLLGAGIIGSALLLPSEEGYELIPGEDWRRVGDQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C889</accession>
    <name>A0A8H6C889_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005320</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148212.1">
        <property type="nucleotide sequence ID" value="XM_037296230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C889"/>
    <dbReference type="GeneID" id="59333726"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF32">
        <property type="entry name" value="ARGININE METABOLISM REGULATION PROTEIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="596"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
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        <location>
            <begin position="305"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="315"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="630" mass="71194" checksum="D59D61610F421D91" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8A1</accession>
    <name>A0A8H6C8A1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148023.1">
        <property type="nucleotide sequence ID" value="XM_037296842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8A1"/>
    <dbReference type="GeneID" id="59334344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10938">
        <property type="entry name" value="CE4_HpPgdA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037950">
        <property type="entry name" value="PgdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561:SF1">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="29"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="316" mass="36196" checksum="FEFD37EAF99C7793" modified="2022-01-19" version="1">MAQKQALCCISVDIDAVAGWLGSYGGEDSTSDISRGLFAGTVGVRRLLKLFAKYEIKTTWFIPGHSLETFPEECAMIRDAGHEIGLHGYSHENPVKMNLEQQHAILDKTYKDLTKFCGKPPRGNVAPWWEASQEGAELMLKYGVEYDHSFSHHDCLPYYLRTGDKWTPIDYSKHPDHWMKPLEKGPDTGLVEIPANWYLDDLPPMMFIKKAPNSHGWVNPKDVEDLWRDHFDFFYREYDDFCFPVTIHPDVSGHPHVIFMLERLIEYISSKPGVKWVKMEDICDDFKSKNKPPKGAMLPAEHGAILDDPNIQLQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8J4</accession>
    <name>A0A8H6C8J4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005970</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148054.1">
        <property type="nucleotide sequence ID" value="XM_037296873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8J4"/>
    <dbReference type="GeneID" id="59334375"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23954">
        <property type="entry name" value="AMO1_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21099">
        <property type="entry name" value="RAD201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21099:SF2">
        <property type="entry name" value="SI:CH211-113E8.11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="4">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="343"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="345"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="398"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="494"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="574"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="608"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="713" mass="76415" checksum="28F47FE10164AD77" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8L1</accession>
    <name>A0A8H6C8L1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148433.1">
        <property type="nucleotide sequence ID" value="XM_037296451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8L1"/>
    <dbReference type="GeneID" id="59333948"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="95"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="536" mass="60312" checksum="21D671FE8A9DF56D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8M9</accession>
    <name>A0A8H6C8M9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005326</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148218.1">
        <property type="nucleotide sequence ID" value="XM_037296236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8M9"/>
    <dbReference type="GeneID" id="59333732"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF63">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G00630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="3">
        <location>
            <begin position="46"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="436" mass="48446" checksum="1EDD0CD1C1A09BD1" modified="2022-01-19" version="1">MDNVPNVATRTLSRRGHTYATGAKKTLENVINNLWDPEDNPDGFVSLGVAENALMHEELASYVKDSIQDLQTHAFTYGDSFYGSKRLRSAIAQFVNHRFQPNQAVKQEHVVATAGLTNSLEQLAYALGDPGDGFLIGRPYYTSLPKDFGSRAGIQSIGVAFGDVDPFSMDAILKYEEALIEARKDGVPTKALFLCSPHNPLGRCYPREVLVGLMKFCQQHKIHLISDEIYALSVWQNPEVPDAVTFESVLSIDTSGIIDPGLVHVLWGMSKDFGANGLRIGCIISQHNHPLLEAVKANSLFTYPPSLSDHITSTLLEDTSYTETYIRTNQLRLAENYAFATSFLKHHNIPYSAKVNAAFFLWVDLKPLFSNVATEEERSKQGKELSGAIMERLMAKKVFVANGEAFAGEEPGWFRVVFSQPKAYVEEGLRRLMSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C929</accession>
    <name>A0A8H6C929_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148565.1">
        <property type="nucleotide sequence ID" value="XM_037295867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C929"/>
    <dbReference type="GeneID" id="59333361"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="253" mass="26876" checksum="4FAEE17ECE1F9AE5" modified="2022-01-19" version="1">MAQAEMVIQGVEGATQAVVGVADAAAHIMDVARDEAAKCKEAARNDNNEFNIRKNFLDNAVNSIKTATCGQYNIVIATDQQKDDFQNLKGQILPMDLLDVEVSQGKIVNFEVYVFDTGNYLRHGKYEQDSWWYSGESKKWYDPMAMHVDFNNAQKKLDPTAIKKQQDDKAASDKTAADAAAAAKAQQDRASASNAAALHTDAAGKAQAAQATGGTAGSTPAPSAPAAPGFTDHLMSAAAGPMMSMFVGKAVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9B6</accession>
    <name>A0A8H6C9B6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005105</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148715.1">
        <property type="nucleotide sequence ID" value="XM_037296017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9B6"/>
    <dbReference type="GeneID" id="59333511"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="33"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="215" mass="24254" checksum="E455FAFBE39E762C" modified="2022-01-19" version="1">MPTATEQPPKVSQASLLAATWSLFAIAVLFFLARIAIRLKISRRLYFDDAWASLALLILLGLALVLTVAIPSMYQVLNVGAGLAEPDTDFMKNATFYLKLQFALTYLYWSCLWAVKACFLSFYHRLTKNLKYCRQVWWATVIISVLSYIGAIITRRKMNSSDDEMSPKGPRLSTHPTAIQLDERSREIAVHQNGTIELAGRGDSTERILRGYEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9G4</accession>
    <name>A0A8H6C9G4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148749.1">
        <property type="nucleotide sequence ID" value="XM_037296051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9G4"/>
    <dbReference type="GeneID" id="59333545"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05132">
        <property type="entry name" value="RasGAP_GAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000593">
        <property type="entry name" value="RasGAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149:SF17">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN WITH IQ MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03836">
        <property type="entry name" value="RasGAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143885">
        <property type="entry name" value="RGC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="1">
        <location>
            <begin position="134"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="728" mass="83810" checksum="1AF9EB3DFD21DDF8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Y7</accession>
    <name>A0A8H6C9Y7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149030.1">
        <property type="nucleotide sequence ID" value="XM_037294983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Y7"/>
    <dbReference type="GeneID" id="59332473"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025444">
        <property type="entry name" value="Monooxy_af470"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13826">
        <property type="entry name" value="Monooxy_af470-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="260" mass="28560" checksum="1E66CCE3C15E7635" modified="2022-01-19" version="1">MADLLLANFSISTWLAAGGCAQSILFLTLPRPVALLPAVILLLLRLAKGALITLGYIHNTYTDGAFLYKSTAPIPDEDGSIPAKAADKGLVVFIVGANTNHPMGLFAPGFANVGDYFQNMWKDAEADREKWGYLGKANTMQMATAGGNALVTISYWKDIEHLHAFAHGPTHRLGWDWWAAQNKKYPHIGIMHETYAVPKGAWENIYQNCHPIGMAQIKYPIRESKDAPLKLVSTITQAKGGKWRTMTSRMGGKEDEGEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA54</accession>
    <name>A0A8H6CA54_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEY1</name>
        <name evidence="12" type="ORF">HO133_003562</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">Enriched in the cortical ER. Concentrated in punctae along the ER tubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA54"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000727">
        <property type="entry name" value="Protein SEY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="767"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="771"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="791"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="11">
        <location>
            <begin position="49"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="664"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="685"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="59"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="855" mass="96577" checksum="0B15E4FC8424E91F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAM5</accession>
    <name>A0A8H6CAM5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heat shock 70 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003784</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149394.1">
        <property type="nucleotide sequence ID" value="XM_037294706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAM5"/>
    <dbReference type="GeneID" id="59332195"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000058">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000028">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000016">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="632"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="632"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="661" mass="71078" checksum="43FA62F71A3E9F70" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAN1</accession>
    <name>A0A8H6CAN1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149515.1">
        <property type="nucleotide sequence ID" value="XM_037294134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAN1"/>
    <dbReference type="GeneID" id="59331622"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="118" mass="12174" checksum="AE409CB7A0BC8C07" modified="2022-01-19" version="1">MPSHTQTPASYGSSSKKPVCYAGSTEVTQPESSGTSDNIILALFKGLSGEEFGPVFVVTALNFLAFWAGLIDSGKIAVLLKFFVGLIDGGDVGITIPGVLLDCFGNFSTGQIVGALVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAT4</accession>
    <name>A0A8H6CAT4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003674</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family. ADAT3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149284.1">
        <property type="nucleotide sequence ID" value="XM_037294596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAT4"/>
    <dbReference type="GeneID" id="59332085"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF156">
        <property type="entry name" value="INACTIVE TRNA-SPECIFIC ADENOSINE DEAMINASE-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="226"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="448" mass="48833" checksum="32D50D7821C078A2" modified="2022-01-19" version="1">MGSPSSSTTKPSQPVNALRGAYDGEGGHLVSLPTKVELQSSCQTIEVFVATIPLKSASTVLKLVQAAIQDAAAANLQHLRKLVKPEHLPPCLRRLGEISESHNPATEPAGLPRFPRNYNGSVPNGYQTATFIAVNGHAEASQQPALHYLVCPTSVVAVEDLHTIFSSMEPSLGVDFEPHLRIIPVPLYPPSSEKQAKDWSQEYWPTVYKGGNPFGPHPALVSRALEDVHSRTGRCMSLARRAGEEVSLAAKGEPIGAVIVDRSGGEDASIIAVAGDARWHHMDKAEEPGSGNVTAHAVMRAIGMVARKRRAPLHKGLAAELYSEESDLDADRPLTPLESDVYTASTVARDGYLCLDLELYITHEPCVMCSMAILHSRFSRVIFGQRMLRTGGLTAVSQNNSGFDIKNDDQGLGYGLFWRQELNWRHLAWQWLTDDDLQTCISSQDVHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB96</accession>
    <name>A0A8H6CB96_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149692.1">
        <property type="nucleotide sequence ID" value="XM_037294311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB96"/>
    <dbReference type="GeneID" id="59331800"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="342" mass="38090" checksum="FD0F6BFC74ECA147" modified="2022-01-19" version="1">MDHVLPALLKRLPILQSFSYVGPLSAKVLAAIVQVDSLRVLQVRNGDDVLKGPATPTPIVIIPWTDITLDWSALASLKGLQALEIGRLIRREARGLAKGVASLNLRRLRLSFWGWEYANINSSITMRSTAHTSALVMFLDTLTTLDLGGGQICRGLPSTLEHLVLIDKYRTWIPYLHQFIATAILPCENLEIPSTTINVSGRCHDTLTKMGLPAFHKIVGVSSWQQLSYDEEMEIPSGEVLQTNPYPKPLRNIVKTLDRVIAAAEGPGNYRMSMESVRERQFRSDAIIVRPCEEEHGPSAAERGPHAQDASVMDLAADFAALSLDETWWAHVLRSWGIWLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBM9</accession>
    <name>A0A8H6CBM9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002899</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149902.1">
        <property type="nucleotide sequence ID" value="XM_037293824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBM9"/>
    <dbReference type="GeneID" id="59331311"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF8">
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    </dbReference>
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        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="6">
        <location>
            <begin position="7"/>
            <end position="392"/>
        </location>
    </feature>
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        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="168"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="404"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="481"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="410"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="210"/>
        </location>
    </feature>
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        <location>
            <position position="218"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <position position="221"/>
        </location>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <position position="243"/>
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        </ligand>
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        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBT9</accession>
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        <recommendedName>
            <fullName evidence="4">MIF4G domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="5" type="ORF">HO133_002975</name>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000593566">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="Upf2/Nmd2_C"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="486"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="694"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="175"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="283"/>
        </location>
    </feature>
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        <location>
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            <end position="1057"/>
        </location>
    </feature>
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            <end position="1142"/>
        </location>
    </feature>
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            <begin position="1159"/>
            <end position="1243"/>
        </location>
    </feature>
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            <end position="951"/>
        </location>
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            <end position="1023"/>
        </location>
    </feature>
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        <location>
            <begin position="1111"/>
            <end position="1123"/>
        </location>
    </feature>
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        <location>
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            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1233"/>
            <end position="1243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1243" mass="140036" checksum="727DDBDDC0D8AB02" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCE1</accession>
    <name>A0A8H6CCE1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002554</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150309.1">
        <property type="nucleotide sequence ID" value="XM_037293480.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59330967"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000002">
        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein Prp31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000003">
        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027105">
        <property type="entry name" value="Prp31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019175">
        <property type="entry name" value="Prp31_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09785">
        <property type="entry name" value="Prp31_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="274"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="388"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="579"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6220874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="604" mass="64738" checksum="97C2E5C43CBC2585" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCN8</accession>
    <name>A0A8H6CCN8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150296.1">
        <property type="nucleotide sequence ID" value="XM_037293467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCN8"/>
    <dbReference type="GeneID" id="59330954"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="81" mass="8489" checksum="C3CB1FC2272DA461" modified="2022-01-19" version="1">MSQSSGSGGKGYDVTSSGTNSQGNRYDSRDYTPSTGGSQANDNTYHYSNNDGSYYYSNSNGSTYHNDGNGGSKYTAPGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCP1</accession>
    <name>A0A8H6CCP1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002476</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150231.1">
        <property type="nucleotide sequence ID" value="XM_037293402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCP1"/>
    <dbReference type="GeneID" id="59330889"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033994654" evidence="2">
        <location>
            <begin position="21"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="588" mass="58648" checksum="3863F7A88C496E60" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCQ5</accession>
    <name>A0A8H6CCQ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="8">Delta-1-pyrroline-5-carboxylate dehydrogenase</fullName>
                <shortName evidence="8">P5C dehydrogenase</shortName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="7">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
                <ecNumber evidence="7">1.2.1.88</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_002705</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.88" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150459.1">
        <property type="nucleotide sequence ID" value="XM_037293630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCQ5"/>
    <dbReference type="GeneID" id="59331117"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00374"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07123">
        <property type="entry name" value="ALDH_F4-17_P5CDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000006">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000005">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005931">
        <property type="entry name" value="P5CDH/ALDH4A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050485">
        <property type="entry name" value="Proline_metab_enzyme"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01236">
        <property type="entry name" value="D1pyr5carbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862:SF1">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 7" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="63"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="549" mass="59568" checksum="23B19F3E49D05B84" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDG6</accession>
    <name>A0A8H6CDG6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150815.1">
        <property type="nucleotide sequence ID" value="XM_037293162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDG6"/>
    <dbReference type="GeneID" id="59330649"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="180" mass="19284" checksum="20363FEED5AF601D" modified="2022-01-19" version="1">MRHPEAMFTHQELPSLLSSSEQGGSLIHTVNIAPLSLLLASMDPAPTANPHAELVTECFQECPALETPARNRSPTTPPDLELLKGSGWGGVIEPAVDKEEDHSQEGKVADVLLDEGCEEGNVPLGSQGWLPGLAGHGGDRISRGPQTSAVEDLSRLKKVRGCTLPSLKELGLDMYVKKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE90</accession>
    <name>A0A8H6CE90_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL13m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001753</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151220.1">
        <property type="nucleotide sequence ID" value="XM_037292681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE90"/>
    <dbReference type="GeneID" id="59330167"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000007">
        <property type="entry name" value="50S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="165" mass="18809" checksum="18CB2ACD57A72ED6" modified="2022-01-19" version="1">MSQTIGKTRLAYSRAWHHVSLSTPDTPPLGRLASQIAIILMGKHKPIWDPSTDCGDYVVATGCTDVYATGRKMQQKLYYKQTTRPGALKSLNMEGMMRKWGGGEILKRAVSGMLPKNKLRKPRLERLKVFEGATHPYKENLIKFDGRLRREEPEVKREILKKKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEG5</accession>
    <name>A0A8H6CEG5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151374.1">
        <property type="nucleotide sequence ID" value="XM_037292835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEG5"/>
    <dbReference type="GeneID" id="59330321"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="169" mass="18600" checksum="860512D8D3CC06F0" modified="2022-01-19" version="1">MNGLELDRRNESPISYQKPDESEKYAYYRQPSGAQTSQIPMASEPATNATESTLPTSDCDNLSKTYVSTIGTHTEFNVYCNSDLDGADFLGVFVSFFEDCMKTCASFNYYQTSNGTQTANAACYGMAFYLDYHENPQDLGRSCFLNDASGITATQRDHTSAASLIQSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CET1</accession>
    <name>A0A8H6CET1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbonic anhydrase</fullName>
            <ecNumber evidence="5">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001894</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 5">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151361.1">
        <property type="nucleotide sequence ID" value="XM_037292822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CET1"/>
    <dbReference type="GeneID" id="59330308"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03379">
        <property type="entry name" value="beta_CA_cladeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175:SF3">
        <property type="entry name" value="CARBON DISULFIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="169" mass="18221" checksum="9BC7DFDF142B9BBF" modified="2022-01-19" version="1">MATPIQANLKNKNARYASNFKEGQLALPPAKKYAVVTCMDARIDPAAAYGINLGDAHVIRNAGGNARDALRSLVISEQLLGTNEILLIKHTGCGMLTFKNEDAHAVVEKNLGAAGQQELSGLDFQPFPDLEKAVEDDVKYLKASKAIPDSIIISGWVYEVETGKVNQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF31</accession>
    <name>A0A8H6CF31_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151719.1">
        <property type="nucleotide sequence ID" value="XM_037292300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF31"/>
    <dbReference type="GeneID" id="59329786"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF40">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="3">
        <location>
            <begin position="155"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="396" mass="44585" checksum="E981CFE412856505" modified="2022-01-19" version="1">MNLSLKLQSPEDYKIFAAGFPYADKCPNGFLGPTAYSAIFLEHQGKLGMDLLDPCKSYHRHLAASALSTPSHDISKCHSVHIHTPNEAAIRLALNILNALPSQDVCDRLLDRYVVFEDLVTHKPTLTFAHKSLWDTYGPYLTAPKDPKKLSFVSEKMCRNAFCYLLWKRHGTLVSSVTALGLHRVVDDGIPDTFMITELHKRQFARVFVREKTVASLTGRPPLLGRRHSTCQLPLDLSDEQLMADEPELSNIKSKLDANGWNTSGELYASTWARALLLMSLVREEVLEISLSISDDCSELLPEYAYPEPKLSKTGQHCSQTMRRCEELYARMPEGIRFKAGKIPTSTHVLPRQIGLHLVYLHSLFLLERLSISRTYASGQRLVDLAIQILDDVLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFD4</accession>
    <name>A0A8H6CFD4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151899.1">
        <property type="nucleotide sequence ID" value="XM_037291537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFD4"/>
    <dbReference type="GeneID" id="59329017"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="86" mass="10055" checksum="372533C661C98A93" modified="2022-01-19" version="1">MEKTLDHYKSNGSAWDFVGRDLSRVRPEPGRIFPEKEIKREDENLQNFLTQHNAYMEAKQENAARENGSQDEGEMDNEIQNIQLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFF7</accession>
    <name>A0A8H6CFF7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151639.1">
        <property type="nucleotide sequence ID" value="XM_037292220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFF7"/>
    <dbReference type="GeneID" id="59329706"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034817507" evidence="1">
        <location>
            <begin position="28"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="156" mass="16661" checksum="D5FC41B89D81B76A" modified="2022-01-19" version="1">MQSAILNLSSILTLLFLPLQILNVGSALPSDQDVPNGQERVGECDSIGNSLMPYISPTTHGGAIPPSPGNAVDGNMTSNLRIVDYTMSMFVPSADATHSQPRMYKQMYATAVNLSTSVVPSVRITFTYGAFTLTLTTLARVVPLDVVIAVLQFQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFL4</accession>
    <name>A0A8H6CFL4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TATA-box-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001380</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151729.1">
        <property type="nucleotide sequence ID" value="XM_037292310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFL4"/>
    <dbReference type="GeneID" id="59329796"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04516">
        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000002">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="26408" checksum="1D443480E499C28A" modified="2022-01-19" version="1">METLTTHPSNAQQATAFTSPASLSFPGGAVAMTIPGQQEGNGVDPGASGGAPITPFSLPDTAGQGVSGIVPTLHLRLKDVRSRNIVATVNLDCRLDLKTIALHARNAGIREPKTTALIFASGKMVVTGAKSEDDSRLASRKYARIIQKLGFNARFTDFKIQNIVGSCDIKFPIRLEGLASRHHHFSSYEPELFPGLIYRMIKPKIVLLIFVSGKIVLTGAKVREEIYTAFEMIYPVLNDFRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG17</accession>
    <name>A0A8H6CG17_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phytoene desaturase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phytoene desaturase (3,4-didehydrolycopene-forming)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_000842</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Carotenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the carotenoid/retinoid oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152140.1">
        <property type="nucleotide sequence ID" value="XM_037291778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG17"/>
    <dbReference type="GeneID" id="59329260"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016166">
        <property type="term" value="F:phytoene dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000171">
        <property type="entry name" value="zeta-carotene-forming phytoene desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014105">
        <property type="entry name" value="Carotenoid/retinoid_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02734">
        <property type="entry name" value="crtI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734:SF1">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="9">
        <location>
            <begin position="18"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6222794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="571" mass="63636" checksum="78474E8E607F20C8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG85</accession>
    <name>A0A8H6CG85_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001057</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152351.1">
        <property type="nucleotide sequence ID" value="XM_037291989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG85"/>
    <dbReference type="GeneID" id="59329475"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF382">
        <property type="entry name" value="LOW-AFFINITY METHIONINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="483"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="612" mass="68133" checksum="0A0BC2F77DB046B5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH08</accession>
    <name>A0A8H6CH08_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hypercellular protein HypA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000191</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152566.1">
        <property type="nucleotide sequence ID" value="XM_037291131.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59328610"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058565">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058567">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058568">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058564">
        <property type="entry name" value="TPR_TRAPPC9_Trs120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26254">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26280">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26282">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26283">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26251">
        <property type="entry name" value="TPR_TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 TPR region" evidence="5">
        <location>
            <begin position="401"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="6">
        <location>
            <begin position="736"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 third Ig-like" evidence="7">
        <location>
            <begin position="1130"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 fourth Ig-like" evidence="8">
        <location>
            <begin position="1290"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="181"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="360"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="611"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="894"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1089"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1388"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1429"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="618"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1394"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1432"/>
            <end position="1452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1452" mass="157464" checksum="7BF4A638189EBD16" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHW2</accession>
    <name>A0A8H6CHW2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000473</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152847.1">
        <property type="nucleotide sequence ID" value="XM_037291412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHW2"/>
    <dbReference type="GeneID" id="59328892"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="242"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="35"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="495"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="508"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="535"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="555"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="588"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="663"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6223630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="693" mass="77059" checksum="AB6C3D117DCEB36E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ11</accession>
    <name>A0A8H6CJ11_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Allantoicase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010979</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the allantoicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153462.1">
        <property type="nucleotide sequence ID" value="XM_037301828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ11"/>
    <dbReference type="GeneID" id="59339369"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004037">
        <property type="term" value="F:allantoicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000059">
        <property type="entry name" value="Probable allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00813">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005164">
        <property type="entry name" value="Allantoicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015908">
        <property type="entry name" value="Allantoicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02961">
        <property type="entry name" value="allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12045">
        <property type="entry name" value="ALLANTOICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12045:SF3">
        <property type="entry name" value="INACTIVE ALLANTOICASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03561">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016516">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Allantoicase" evidence="5">
        <location>
            <begin position="80"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Allantoicase" evidence="5">
        <location>
            <begin position="253"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="403" mass="44127" checksum="46878BE4F8E691BF" modified="2022-01-19" version="1">MEIQALSPEIIQDIQSTPTDFIPPDQIDSTFGTTCIGKAPSSPSPGAPPLSDSLSDSPVHTGCLEPSLTITDLVSAPLTSRILSFSDEFFASASNLLNPANPIRRPGHFVESGAWYDGWETRRHNRSPPDWVVIRLGPAAGMVRGVEIDTAFFDGNHAEGVEVWGTYETGEGADERVIEAGYRAWRPLLGKRGCGASRRQAWTVNERTGEVTHVKLCMLPDGGIARFRLYGVAVPVWPKDESEEVELSSALMGGRVVGFSDEHFGKAGNLLMPGRGVDMGDGWETKRSRESGHSDWVVVKLGARGRVRSLMIDTMHFRGNFPQGVRVEGLDAEGEEVVSGDDERWVEVVGMQGCEKDKEHVFADGLLKSVEGRAFTHMKMTIIPDGGVKRFRVFGVRAQERHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ90</accession>
    <name>A0A8H6CJ90_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_011099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153582.1">
        <property type="nucleotide sequence ID" value="XM_037301948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ90"/>
    <dbReference type="GeneID" id="59339489"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42069">
        <property type="entry name" value="HYPHAL ANASTAMOSIS-8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42069:SF1">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="352" mass="39512" checksum="47FD912B28675402" modified="2022-01-19" version="1">MAAASYYQLDSFPNSDPTAGSDLYSGHDYNNHPVHPSEPSRPDLHTVHSNGNSYNCNDDHMRPTEPSRPDLYTLHSNSNSYNSPPQHPMNNMHSSPPPQLSIDSWNARPPHPTDPPTKPLVHSSHVAVPQPLKIQTPNSRLRQRRFQTLKRYLRIGKIVTKVITILFSTVMFAIMLFTVIKYQSTKGEFRGGRTAWPKQPKLWPTFMLLAGAGLTLLLSSITLLSYCCAFDKARRSWKLTVVKYVIHIGAWLVISALYRYEKSTHGVSNDLWGWSCSTEATALQSEFNGVVHFSSLCSVQSKSWEVSLAEVVAKIVFAVGHYVIYRKTRDDEKQHLANGVGGAGTDFLEDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJE0</accession>
    <name>A0A8H6CJE0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010864</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153349.1">
        <property type="nucleotide sequence ID" value="XM_037301715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJE0"/>
    <dbReference type="GeneID" id="59339254"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015227">
        <property type="term" value="F:O-acyl-L-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902603">
        <property type="term" value="P:carnitine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000060">
        <property type="entry name" value="Mitochondrial carnitine carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF4">
        <property type="entry name" value="CONGESTED-LIKE TRACHEA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="109"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="216"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="305" mass="32085" checksum="7B0E6CE39B5D12FC" modified="2022-01-19" version="1">MSSSSSSSTGPSIPSAEEVRTEVVEASKQTLSSFRSLIAGGVGGICAVIVGHPFDLVKVRMQTAEKGVYSGALDVVRKTVAREGLRKGYDVGQKLVRAFSPQTNSDAHFTIGQTSTAGFFSAIPMTIITAPFERVKVLLQIQGQKQLAPGEKPRYSGGMDVVRQLYKEGGIRSVYRGSAMTLARDGPGSATYFASYEYVKRKMTPKDADGNPSGELSLTAVMTAGGAAGVAMWIPVFPIDTIKSRLQSAEGRPTIGGTIRGLYASGGFKAFFPGMGPAMARAVPANAVTFLGVELAHKAMNNAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK77</accession>
    <name>A0A8H6CK77_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009950</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153623.1">
        <property type="nucleotide sequence ID" value="XM_037300806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK77"/>
    <dbReference type="GeneID" id="59338342"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052250">
        <property type="entry name" value="PDI_TMX3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46426">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE TMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46426:SF1">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE TMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034526147" description="Thioredoxin domain-containing protein" evidence="7">
        <location>
            <begin position="21"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="730"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="80"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="308"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="233"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="781" mass="87289" checksum="C2D56FD09EEA72F4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKD9</accession>
    <name>A0A8H6CKD9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010025</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153698.1">
        <property type="nucleotide sequence ID" value="XM_037300881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKD9"/>
    <dbReference type="GeneID" id="59338417"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004059">
        <property type="term" value="F:aralkylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051635">
        <property type="entry name" value="SNAT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10908">
        <property type="entry name" value="SEROTONIN N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10908:SF0">
        <property type="entry name" value="SEROTONIN N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="129"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="275" mass="30164" checksum="4EDA91D01AFE8ECA" modified="2022-01-19" version="1">MMPQDLFSDSLVASPESQTPRLDVSEGSTATGNDGYNDFTIHRQEGKRSSAQQLRTNRAKVNERNDLHPYVQTLSLSNLESCVALENAVFSELERCAREKFIYRLTTCPELCLGLFSSTPPNSPNASIPTFSTARPADSASPDLKSVLIAMVISTKTMSPTVTDESMDVAPNWKINLSNTSIHGHREDGRTIAIHSLSVLPAFQGRGLGKTIMKAYQQRMETSGIADRMALLAHDHLEEMYEGMGFENKGKSDVRFAGGGWNSLTYEFAEHGPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKG9</accession>
    <name>A0A8H6CKG9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Autophagy-related protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010379</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Autophagy-specific protein that functions in response to autophagy-inducing signals as a scaffold to recruit other ATG proteins to organize preautophagosomal structure (PAS) formation. Modulates the timing and magnitude of the autophagy response, such as the size of the sequestering vesicles. Plays particularly a role in pexophagy and nucleophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Preautophagosomal structure membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ATG17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154049.1">
        <property type="nucleotide sequence ID" value="XM_037301232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKG9"/>
    <dbReference type="GeneID" id="59338770"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007240">
        <property type="entry name" value="Atg17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28005">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28005:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="8">
        <location>
            <begin position="41"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="494" mass="55056" checksum="0747E8B164912979" modified="2022-01-19" version="1">MASSTSSLQPPSPPPGPFSAAGTASSPLETLIPHLLASKRSLSSINHVWRANELCTSTRTSLERSTITTARTSFLRSGITSQLAVLQQVHRNTEATAKQGRAEFEAVIQVLDEADERLRDTLGKLRSTIVEKGLRPGDEERRSLLDFMNEEGVEGLVGVIKESLDGAGQGMREFEDGNRGFREEVENVRKSLYGDVMKGGSGLVGSASQGRVVQSQERSPIPQILHEMEDRAREMATGLESLVSHYDLCVTAIKQTEGGGDAASKIAGDLPEGVDIGQDAGGAPPEPMGDQERMEMMRVLEEDAGQVEDVVMEIRDHIAEMESLHSRVEMHTSHLATEHVNMTAAFRMLEDLGQKLPGHITRTQIFLMRWDEDKAAIDERLEELEGLTELYDGFLRAYDNLLIEIGRRKDQELEVEQVVQEARTRLEKLYEDEVEEREVFKKEQGDFLPVDIWPGLRDRPLRYSLSSVDDQASRVPDISKSVIYRAIQRVHGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKL8</accession>
    <name>A0A8H6CKL8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH TFE/IIEalpha-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010174</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIE alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153846.1">
        <property type="nucleotide sequence ID" value="XM_037301029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKL8"/>
    <dbReference type="GeneID" id="59338566"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="327"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="361"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="460" mass="51215" checksum="FE8837C0B43A5710" modified="2022-01-19" version="1">MDLAKTLIRSVVRAFYDTPFVLVIDALMVHSALPNEDLAYLLQIPSKELRKLCGKLKEDRLLSVHSRLETREGQQRPINKDYYYVDFHATIDAIKYRIFQLTQKVESMYKPSEEKKDFHCPRCKAQWTELEVLDRPVPEGFECHRCGGLLERKEQLDAGDSTGHAKQSRLMDQLFGLLKMLQQVDSEDIPSNDFDTAFSHAVLVERNIVVNPGQQRVTFNAPEPPPAAVKGMMPQLVAAQLDVSVTTGSERTTAEQAAEAQRKANIAAQNILPVWHTTSTVTGETTVAGKKDGEQQVNGRALLKEEEDEKKISTEDEQMAAYYAEMRREEDMEQERKRVAREQEAASEDEDEGDFEDVLSASRNGTPSSSMSENWKGPPVSQTNGSVKRKGSESGSSAPATNTSTPIGSGQAIEEGDGPVLKKVKFEDRKNGASAVKSEAEEQVDNDSDEDEEANFEDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKM7</accession>
    <name>A0A8H6CKM7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010400</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154070.1">
        <property type="nucleotide sequence ID" value="XM_037301253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKM7"/>
    <dbReference type="GeneID" id="59338791"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF60">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="71"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="516" mass="56234" checksum="A10798D610E25236" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLB6</accession>
    <name>A0A8H6CLB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_010408</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154078.1">
        <property type="nucleotide sequence ID" value="XM_037301261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLB6"/>
    <dbReference type="GeneID" id="59338799"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17961">
        <property type="entry name" value="DEADc_DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF21">
        <property type="entry name" value="DEAD-BOX HELICASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="24"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="55"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="250"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="345"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="607"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="24"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="383"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="607"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6225211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="637" mass="70914" checksum="F8EB147497BDDFA3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLJ5</accession>
    <name>A0A8H6CLJ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_009683</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. PFA5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154392.1">
        <property type="nucleotide sequence ID" value="XM_037300544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLJ5"/>
    <dbReference type="GeneID" id="59338078"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF23">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="18"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="178"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="101"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="419"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="425"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="451"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6225683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="483" mass="53904" checksum="E319C8629A141522" modified="2022-01-19" version="1">MAPNASINKATNLWVSRVIPLILIGLVGYVSWVEIKLLCVDYLLRPSTTLHTPRPGPAIGILVIYSVLLLLFVFTYARLLFTVTVTPGYVPRCSQWRALRESKTKAKGRRNQRHRKRSSQSSNGSTGENAGGESAENGRISGHNYGGGASTAPTTTEAAPGLQDFYSRDAFVCQTDGRPNWCSTCLNWKPDRAHHCREIERCVRKMDHFCPWVGGVVSETSFKFFIQFVGWSAIYCVFNLTVAAYFLAEYRRETHSLHVHWLVVVCLGALFGLFNLGMTGSSLQFALINTTTIENLSRRTLVWTLAIHMPKPPETSIGFRTVSFSTATLTTESDAPAEEQASGAIKTFAILHTKPGENPFDLGPYQNFKTVMGDHWYDWLLPIRFSPCCNHDRRDGQFAMGPVVQRMRAAAGIASVEEISDEKMHRKPKRRRRQSHRARAAVAAPETDSNQDEKKGRDTGHNRDEADDIDLEAGLGHTNGHVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLZ6</accession>
    <name>A0A8H6CLZ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154408.1">
        <property type="nucleotide sequence ID" value="XM_037300560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLZ6"/>
    <dbReference type="GeneID" id="59338094"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="653" mass="73982" checksum="8BBFE40A64840A35" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMJ2</accession>
    <name>A0A8H6CMJ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154948.1">
        <property type="nucleotide sequence ID" value="XM_037300122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMJ2"/>
    <dbReference type="GeneID" id="59337656"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="365" mass="41307" checksum="17BD7541A6AC7524" modified="2022-01-19" version="1">MDRFIATQRIPRGSRLSGWLHGSSVRDNSGRRPPIHTRKTSHTSTYCFAAAERGHQRHAPGIRSYPSTRGLIDPVHSPTYGPRQPTYGSDSRHEVYLERLCAELGSAPRERCSKRRHQQKSRRAPAKNERLCFPQIKDSKMRQKIIGCLFFGTLLTIVLTTYLALAISESLRGATFHGLFIFFILVLTIFFCHYLIRLCMLAFEPRMRFARARKCQSPLNGDEEAGFAQPRQPIPVVLARDEELGLHDDNRRVEEEDIDEALPPPPPAYGLWRSSVRADPNLIHWQAVRDNNDVQQEAREMIFAGTGHRPPSYTSHDRTEGVFDDRVEGVLEPEPVAAPLPLLAERRAAGTVLSRGGTVRMARSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMQ1</accession>
    <name>A0A8H6CMQ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell wall mannoprotein PIR1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008938</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154627.1">
        <property type="nucleotide sequence ID" value="XM_037299801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMQ1"/>
    <dbReference type="GeneID" id="59337333"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF2">
        <property type="entry name" value="COVALENTLY-LINKED CELL WALL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034873188" description="Cell wall mannoprotein PIR1-like C-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="4">
        <location>
            <begin position="70"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="276" mass="27540" checksum="D98BA89DBEBDF582" modified="2022-01-19" version="1">MKYAVALSALAAAATCVSAQTPDGCSSDYTGYFEFNPVPISGASKRDVAPLHKRQTQTCTSEPYSTLKGGVLTDQCERTGEIVANAQFQYDLHLQNNAINTGGFSICTNNTLAIAGSAVFYHCLSGTFYNLYDASLGAQCSPVYLEIIGCTPPADAGSDCPAGTPTSVSSSTSPSSISSTSVASVPSSTSGTSPAITPAAVAAPANAVLVVSMPYTYTSSNATASATATGTGVVTPGSTGTAKTSPIAFHGGAASLAMGGKLLVSAVAIAALAFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMU3</accession>
    <name>A0A8H6CMU3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">STI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009168</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Part of a larger complex that includes HSP70, HSP90, and immunophilins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154855.1">
        <property type="nucleotide sequence ID" value="XM_037300029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMU3"/>
    <dbReference type="GeneID" id="59337563"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000004">
        <property type="entry name" value="Putative stress-induced-phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000010">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000020">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000002">
        <property type="entry name" value="Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000027">
        <property type="entry name" value="stress-induced-phosphoprotein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041243">
        <property type="entry name" value="STI1/HOP_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17830">
        <property type="entry name" value="STI1-HOP_DP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="8">
        <location>
            <begin position="139"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="332"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="8">
        <location>
            <begin position="528"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="198"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00064323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6226302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="579" mass="63783" checksum="0D9C89C8DAC08F6C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNQ9</accession>
    <name>A0A8H6CNQ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">mitochondrial processing peptidase</fullName>
            <ecNumber evidence="5">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_009322</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins. Preferentially, cleaves after an arginine at position P2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155009.1">
        <property type="nucleotide sequence ID" value="XM_037300183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNQ9"/>
    <dbReference type="GeneID" id="59337717"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000002">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="16">
        <location>
            <begin position="50"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="17">
        <location>
            <begin position="202"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6226456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="477" mass="52908" checksum="059002635DE0F7BB" modified="2022-01-19" version="1">MASRRLALNLNQALRSRAALKSIQPIRGFATPIHQGINTESTTLSNGLTIATEHSPWAQTSTVGVWIDAGSRAETDKTNGTAHFLEHLAFKGTKNRTQQQLELEIENMGGHLNAYTSRENTVYYAKSFNSDVSATVNILSDILQNSKLEPQAIERERDVILREQEEVDKQLEEVVFDHLHATAFQGQPLGRTILGPKENIASIQRDDLVGYITKNYTADRMVLVGAGGVPHKQLVELAEKYFSNIPMEPPTRAAASSAAAQKQIPDFIGSEVRVRDDTIPTANIAIAVEGVSWKDDDYFTALVTQAIVGNWDRAMGNSPYLGSKLSTFIHENKLANSFMSFSTSYSDTGLWGIYLVTENKTQIDDLVHFTLREWSRLSYNVTEAETERAKAQLKASILLSLDGTTAVAEDIGRQIVTTGRRMDPEEVERVVGSISEKDVMSFARRKLWDQDVAISAVGSIEGLLDYNRIRNDMSRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPM3</accession>
    <name>A0A8H6CPM3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">homoisocitrate dehydrogenase</fullName>
            <ecNumber evidence="12">1.1.1.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_008462</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R,3S)-homoisocitrate + NAD(+) = 2-oxoadipate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:11900"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57499"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.87" evidence="12"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155329.1">
        <property type="nucleotide sequence ID" value="XM_037299328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPM3"/>
    <dbReference type="GeneID" id="59336858"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047046">
        <property type="term" value="F:homoisocitrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009085">
        <property type="term" value="P:lysine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000012">
        <property type="entry name" value="Homoisocitrate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF48">
        <property type="entry name" value="HOMOISOCITRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="13">
        <location>
            <begin position="7"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6227021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="358" mass="38251" checksum="A1074061C443E80A" modified="2022-01-19" version="1">MASGSLRIALIPGDGIGREVIPAGRRVLEAIASTFKLDLSFLDLPAGYETFQQTGNALPDRTVETLKKECNGALFGAVSSPTTKVAGYSSPIVALRKKMDLYANVRPVKTTASSKMPIDLVIVRENTEDLYVKEERTYEASDGSGKVAEAIKRISERASHRIASMAGEIALRRQKIRDTGVQSVHSSPLITITHKSNVLSQTDGLFRETARAALADPKYKSVDVEEQIVDSMVYKLFRQPSSYDVIVAPNLYGDILSDGAAALVGSLGLVPSANVGDGFVIGEPCHGSAPDIMGKGIANPIATIRSTALMLEFLGHEEAAAKVYAAVDANLDEGKLLTPDMGGKAGTVEVIEDVMRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPR0</accession>
    <name>A0A8H6CPR0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Potassium channel tetramerisation-type BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008801</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155665.1">
        <property type="nucleotide sequence ID" value="XM_037299664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPR0"/>
    <dbReference type="GeneID" id="59337196"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18316">
        <property type="entry name" value="BTB_POZ_KCTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF136">
        <property type="entry name" value="GH08630P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="2">
        <location>
            <begin position="23"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="220" mass="25101" checksum="59BC4EA6C20F7541" modified="2022-01-19" version="1">MTTPTPQTASASAPAPTPSNTMTLQVGECRFVTRPATLTRGSDFFARQLSGRWPDIQPDGSYFIDADGDVFQHVLRYLRHEIMPIFYDNVKGHDYALYLAVLEQARYFGVEPLQKWLEEQKYLQAVKIVHSARVLDGIEDVCGPTGSHVKIEHHVTWKSRKVYVCRRAITGHMGNRDACGRLCRNARGDADDEYEDEEVLKILEVRKTTMLDAKMRVEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ51</accession>
    <name>A0A8H6CQ51_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008633</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155499.1">
        <property type="nucleotide sequence ID" value="XM_037299498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ51"/>
    <dbReference type="GeneID" id="59337028"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="33"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="327" mass="37002" checksum="27226F67C5CD105F" modified="2022-01-19" version="1">MVARVPTGVPSRANEIIATAIACSILSTVFVAIRIYTRSRIIRAIGWEDYAALITLPFCIAYGTLLGISTRYGMGLHAWDFPGDLREQYQKWIFIASCTYLPSLLGYKMSILFLYLRIFNVDRIFRYCTWAAMFVTFGYLFSNFWTLIFGCQPIAKYWDPETPGHCIFTLKADYGYGSLNFITDLLIFVLPLPMVWRIQLSRKERIGVSLIFMIGSVNWAVAIVRFVCAVRDLTSEDRPWLAAETFLWSIIEVNTGLICACVPVLKPFFMHVVPKSVTDRSWSFGRGKVSPTGDALEDERKYSDRLWDDLERGKAGSLGTSASSSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ84</accession>
    <name>A0A8H6CQ84_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155544.1">
        <property type="nucleotide sequence ID" value="XM_037299543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ84"/>
    <dbReference type="GeneID" id="59337073"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051531">
        <property type="entry name" value="N-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43792">
        <property type="entry name" value="GNAT FAMILY, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G00765)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43792:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="195" mass="22224" checksum="18A5AB305EBD6491" modified="2022-01-19" version="1">MSSPTIHRPSDTITTERLLLRALSPTDREGVFAIRSEPRVLYWTEPDTLEKSDEWLRIRLESEKSMTYAVSLLPSTDNRCPQIIGVTGAHSLPEIGYIFHPSSWGHGYATEALKAWIHMYWQRYPDGHPVLHESERAYLKAVTGPGGEGSRGVLRKCGFRWFREDEVCDERKGAKEKGLSVVLQEFRLARPGVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR01</accession>
    <name>A0A8H6CR01_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155931.1">
        <property type="nucleotide sequence ID" value="XM_037298595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR01"/>
    <dbReference type="GeneID" id="59336122"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF15">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="2">
        <location>
            <begin position="61"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="326" mass="36645" checksum="40B426510DAD8BE1" modified="2022-01-19" version="1">MGNALLLFVIKNSGRSKSTKAGVDLLNRHQNEEHLHTVAEWLSTSDYPSQQTDLFNQCQEGAGQWLFHLKEFQAWLDGTESTLFCPDIPGAGKTMITSIMINNLQQRFQGDKKVGIAYVYCNYKRQTEQTIHHFMASLLKKPVQQQPNLPESTDKSIVHGLARAFIVMDALDECTDTNKPRSILLEEIANLQVQLNTHFFAISRFIPDITAKFRDVATLEIRATDADVQAYLENKISETPVRVSRNRALQEKTRAEEGKPAAGMLSDKVTAKDVKIALRDVLKGSGALEITYTQALERIKGQKPGFRRLAEAVLSWTVCAQRQLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR12</accession>
    <name>A0A8H6CR12_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apurinic-apyrimidinic endonuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_008030</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156234.1">
        <property type="nucleotide sequence ID" value="XM_037298898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR12"/>
    <dbReference type="GeneID" id="59336427"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="10">
        <location>
            <begin position="255"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="544"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="544"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6228300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="595" mass="65991" checksum="EA2AD954A94785F1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRH0</accession>
    <name>A0A8H6CRH0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type Ca(2+) transporter</fullName>
            <ecNumber evidence="2">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_008059</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156263.1">
        <property type="nucleotide sequence ID" value="XM_037298927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRH0"/>
    <dbReference type="GeneID" id="59336456"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000031">
        <property type="entry name" value="Calcium-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000040">
        <property type="entry name" value="Calcium-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000028">
        <property type="entry name" value="Calcium-transporting P-type ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="345"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="815"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="888"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="959"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="989"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="605"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="605"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6228329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1032" mass="110530" checksum="AD258A3E4709B8B5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRH5</accession>
    <name>A0A8H6CRH5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007889</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156093.1">
        <property type="nucleotide sequence ID" value="XM_037298757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRH5"/>
    <dbReference type="GeneID" id="59336286"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF43">
        <property type="entry name" value="ASPARAGINASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="210"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="5">
        <location>
            <begin position="210"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="377"/>
            <end position="380"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="465"/>
            <end position="468"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="4">
        <location>
            <begin position="348"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="565" mass="62139" checksum="C327EBA2CB8B3D2D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRS5</accession>
    <name>A0A8H6CRS5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156428.1">
        <property type="nucleotide sequence ID" value="XM_037299092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRS5"/>
    <dbReference type="GeneID" id="59336621"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141673">
        <property type="entry name" value="MOSC N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="230"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="412" mass="45687" checksum="D942AB60686AA24A" modified="2022-01-19" version="1">MAIIFDRKESGMIKLFLDGKLLTNESISLVASPKIGQELWFNALDWDNLDTGFRGSLNRFTMYAGALTPETISLRSVPLTTAIASPHGFPYDRRFMLVKVHDGKTFSPRSKSHANGNLENMTITYYPELALFLQSINSNGTFTVTYDPPDRARRSLEIQLEPEVAHLEEMDVRLHQSPAKGYKMPERVNAWFSECFGYDVMLSYLGPNQRPVLGNLSPNAAQDGGAASKSWLSSITTSIPSLLASKDVEGEGISFADVAAYLVITEESLRDVDSRLQDGTRMDVTKFRPNIVLSGAAAAYEEDYWGSITINPNKKQEKNNVTEIVLTQNCARCVSINVDYSTGKSGTGEANILKKLMKDRRVDKGNKYSPIFGRYGFLKGAAAGQVIDVGDEVTVSKTNKERTTFRRSSVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F779</accession>
    <name>A0A8H6F779_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006713</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147046.1">
        <property type="nucleotide sequence ID" value="XM_037297610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F779"/>
    <dbReference type="GeneID" id="59335114"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF1">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6217611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="34260" checksum="D3D8DECC476560F8" modified="2022-01-19" version="1">MSLERKGHHGPSTFDGKSQIDSTSRPAWYAERVESIPDAGRQLLEVYSGIPAERVLPHVLAIVRTPLYRDKAFDIWSYACIGQVRFLDFTLPKLPYWPRILPRLRAGASFVDAGCCFGQEIRFLVFKEGIAATQLYGFDLEPAFLDLGYELFLDRDELHAQMLSGDVLADSMAPDGKGLKAVEGKMDIVHAASLLHSWGWDDMVTATKRLVSLTRKLPGSMIIGNQMGSLDAGQYPMPTGTGLNYRHNVESMERFWAQVGKETGSSWKVESDMFLPPVVKENMAHSWARSDPNLRMIWFCAERIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F873</accession>
    <name>A0A8H6F873_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006216</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147689.1">
        <property type="nucleotide sequence ID" value="XM_037297115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F873"/>
    <dbReference type="GeneID" id="59334617"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051609">
        <property type="entry name" value="NmrA/Isoflavone_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706:SF7">
        <property type="entry name" value="CIPA-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706">
        <property type="entry name" value="NMRA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="4">
        <location>
            <begin position="24"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="349" mass="38069" checksum="D00DB5E48950BE8A" modified="2022-01-19" version="1">MASKYARDMPAGFTNRIERVAIVGASGQMGEHIAQALVKSGKHNVTALTRHGSKNEVPSGVKAIPVDYSDESTLVAALKGQQFLVITLAAMAPPDVHSKIVQAAAKAGVPHIMPNLFGLDYSNEALINDTLLSADFKSMLPEIEGAGCSWTSMICSLWYEYSLAMGPIWFGFDFANKKLTLYDDGTTKINLTTWEQCARAVAALLSLKELPEDANDKSLTVNTWRNKPLVISSFLVSQRDIFESWKRVSGDKDSDWTIEKEHSKERYQKGIEAMKSAQDPMSTRMGAAMASFVRAFFPNGGGDYESTRGLDNAKLGLPKEDFDERTAVAKGMVEQGYAAKVYAKATSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8B6</accession>
    <name>A0A8H6F8B6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148141.1">
        <property type="nucleotide sequence ID" value="XM_037296159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8B6"/>
    <dbReference type="GeneID" id="59333654"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF96">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="57"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="276" mass="31672" checksum="73D2E7CC3B27AB8F" modified="2022-01-19" version="1">MSNSNTSSEDPYMLGRNYYASARLAMLHLLIHAQFGFLLHPSIKLPSAIKNQEQLRIADVGTGTGIWIGELTRELPQAHIDGYDISDEQYPPQEWYGPNVSLSKLDIFKPLPKELEGKYDVVHLRFFMTIASDDNVQAVVNNLKGMLKPGGCLQWVERDWMSKFPEKAEKSDDPHTQITCYHRSLFPKVRWINSLPAQFEQCGLEVLDYVTQAPLPVYRKPWNEDCLIGWAAIGDLIKNQEDKKWYRERLAEAAENAKKGWYVDWELVVCVGRKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8R1</accession>
    <name>A0A8H6F8R1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004981</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148591.1">
        <property type="nucleotide sequence ID" value="XM_037295893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8R1"/>
    <dbReference type="GeneID" id="59333387"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034009892" evidence="2">
        <location>
            <begin position="24"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="401" mass="40416" checksum="8BE7ECB07D053E20" modified="2022-01-19" version="1">MRVTKSVVFGLVSLVCTINAIEATGKPVLAKRQAVVTVTNDGCTAICWDLSSSDLGTGTDCDVYCSEGFSTTMPATSTSTSVVISTVTSSSKTALNQPTVVSSTGGDCSAVCTGNLDEGDIDCGFDCGGSFYTAMPTTSTSKSIPTSEVALNRRTVISATSGDCTAICWDLMPGTECDVQCKSDFRTEMPVPSKAARVGLEARENVQAHQVREARLARKFGGLVGRDDSMTVTVTSIPEYCTAGPESSISTTVAGIPTILPMQVPQAAGAFDTSAALSQASAAISAVQQVMPSAASSALSQASAALSSMTAAAPISANATIATPASTGSAPTVSATTTATSTSSETGTPSTTTTGSLVVSEVGSTTRTIVPANGALPEYTVPTGLVLSLLVALAANVAFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9T4</accession>
    <name>A0A8H6F9T4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Signal recognition particle subunit SRP68</fullName>
            <shortName evidence="10">SRP68</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_003015</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). The SRP complex interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the SRP68 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150017.1">
        <property type="nucleotide sequence ID" value="XM_037293939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9T4"/>
    <dbReference type="GeneID" id="59331427"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15481">
        <property type="entry name" value="SRP68-RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.40">
        <property type="entry name" value="Signal recognition particle, SRP68 subunit, RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026258">
        <property type="entry name" value="SRP68"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034652">
        <property type="entry name" value="SRP68-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038253">
        <property type="entry name" value="SRP68_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 68 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16969">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038995">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6220582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="617" mass="68739" checksum="7A770E865987F324" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9T9</accession>
    <name>A0A8H6F9T9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150032.1">
        <property type="nucleotide sequence ID" value="XM_037293954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9T9"/>
    <dbReference type="GeneID" id="59331442"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034501030" evidence="2">
        <location>
            <begin position="23"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="347" mass="37296" checksum="F23B44CE3A89A03B" modified="2022-01-19" version="1">MPAPLAKGLILTISLICAAGLAIYENPQVRQWIDESRRKAAIALYSLGDEVAPQSESRNTSPDASTREDEDPEAVERRRRARHEILERGRMIEERRRSKQAARGKARSFDDLVDRDGALKVEETTATSSAAEAQPEEPGLRLRHIESRAAALGSAYADPFADEMGVPIMDAPPLPQEQEPDTISGSSTPTHPASSASPPILRDSPASPPVPPKPAAYQPQRLLIDTDEVSNHPSEQLLDLTPTTSASSAAADLAELSNEPQPTRSNFWSVNEWAQNSAPAFYSPPQSEAAGSIEEKMENHTGGSHAGTGEHASQVGSEDMDVMSDDEALVSTPGSWTEVGSQVSEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9V3</accession>
    <name>A0A8H6F9V3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Short-chain dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003443</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149746.1">
        <property type="nucleotide sequence ID" value="XM_037294365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9V3"/>
    <dbReference type="GeneID" id="59331854"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF236">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="35161" checksum="F56CBA3AAF1903AE" modified="2022-01-19" version="1">MSQISQLFPPAPKFTEKDLADQAGKVFLVTGGAAGIGLELAKILYAHNAKVWIAARSEQKASKAIASIEASFPKSKGELVFLHLDLNDLMVVKKSAETFLAQETRLDVLWNNAGVMIPPQGSKTKQNYEAQLGTNCLSPFLFTKLLTPLLVETAKTAPRGSVRVVWLGSSAAEMMSPKGGGVDMTNLDYKEDKSAWYKYGVSKAGNVLYSKELAKRHQADGVLSVALNPGYLKTELQRHMSGVGASIFNLFLHPPMYGAYTELFAGLSPKVTLKNSGAWIIPWGRFSSIRKDIEAAGKSKSEGGTGRAAEFWAWSEKQVQPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA05</accession>
    <name>A0A8H6FA05_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Methylenetetrahydrofolate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="12">1.5.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_003290</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes oxidation of cytoplasmic one-carbon units for purine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.15" evidence="12"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149594.1">
        <property type="nucleotide sequence ID" value="XM_037294213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA05"/>
    <dbReference type="GeneID" id="59331701"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01079">
        <property type="entry name" value="NAD_bind_m-THF_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000255">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000012">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035812">
        <property type="entry name" value="m-THF_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="14">
        <location>
            <begin position="20"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6220159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="332" mass="36895" checksum="5E159F1C57BA317B" modified="2022-01-19" version="1">MAAADKKADPMSCQVVLAGAVAKDLLTEVSEGLSKLGKSPLLVGFLANTDPAARKYADWTDRTCRENGFKFEVREVDREDLEANVLAANEDDGVDGIIVYYPIFGNRQDQYLQQVTDVSKDVEGLSHRYIFKLYHNERFLDKNMSQKSLLPCTPLAVLKILDFLRVYNSILPYGNRLFGRSITVVNRSETVGRPLAALLANDGACVYSVDVTGVQQFTRGEGIRKSRHEVVEKEGWGLETCVPLSDVVICGVPGDSFKLPTKLLREGAVCINFSTEKNFEGDVKDKASIYVPAIGKVTIVMLLRNLLRLVQNREVTMDSKEQALKTILSQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAD0</accession>
    <name>A0A8H6FAD0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002771</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150525.1">
        <property type="nucleotide sequence ID" value="XM_037293696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAD0"/>
    <dbReference type="GeneID" id="59331183"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46266">
        <property type="entry name" value="TRANSCRIPTION FACTOR TT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46266:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR TT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="128"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="348" mass="37447" checksum="12E7339A238DDD7A" modified="2022-01-19" version="1">MPRTSLPPTPAASTEIQGKDGSPISATEKSFNLPPPAIESQRNNGTSTAQKQTQSALSPCFSLPPPPTRSRTIIQMKPKPQCSEDSVRSPMQQGLKGSNKKTSTSSSATATAGKKQPSSTSVAGKKIARKTAHSVIERRRRSKMNEEFGTLKAMIPACTDQEMHKLAILQASIDYLRYLEKCIADLKAANNSLSTPLTQPQAPPPRFDAVTPKENDEDDDEDQDDEDVTMGDSGSNCISPSIMAIASKAYAYTSPQSISASPAIEAQSNHHHSSYASSVSTLPSPAFEPQHGHRGPQSQFSLSASTSPTIMPSREQDQEATAALLMLNKDRRYPKGGRAMSVRDLLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAK9</accession>
    <name>A0A8H6FAK9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150164.1">
        <property type="nucleotide sequence ID" value="XM_037294086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAK9"/>
    <dbReference type="GeneID" id="59331574"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47668:SF1">
        <property type="entry name" value="DIENELACTONE HYDROLASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47668">
        <property type="entry name" value="DIENELACTONE HYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G01940)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="246" mass="26491" checksum="072D0B85B34CF84F" modified="2022-01-19" version="1">MSLPSKACCNIPPVEVDYTPKGSDIKIQGMKTYKTGSPSATTAILLIGDVFGNVAQVLQGADLLAYGGESSHQYQVFVPDFLRGQYASHTWFPPDTEEKQKAIGAYFGGHANFGTAKEKIPAVIKEIEQETKGTITKWAAVGLCWGGKVATLAAGSDTPFVAAVSAHPALVDPKDGPSVTIPYCLLPSMHEDKDKVKAFADAINVEKYVETFDDMPHGWMAARADLNDPTAKEGYKRGYETALNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAW0</accession>
    <name>A0A8H6FAW0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Lysophospholipase NTE1</fullName>
            <ecNumber evidence="3 16">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Intracellular phospholipase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_001596</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the NTE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3 16"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151065.1">
        <property type="nucleotide sequence ID" value="XM_037292526.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330012"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000062">
        <property type="entry name" value="Lysophospholipase NTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000007">
        <property type="entry name" value="Lysophospholipase NTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000018">
        <property type="entry name" value="Lysophospholipase NTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 15 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15 16" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11 15 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="695"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="837"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="19">
        <location>
            <begin position="1215"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="275"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="512"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1500"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="15">
        <location>
            <begin position="1219"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="15">
        <location>
            <begin position="1246"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="15">
        <location>
            <begin position="1366"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1507"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="1248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="1366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6221630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1519" mass="168088" checksum="AE78EA08A01457FB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBB1</accession>
    <name>A0A8H6FBB1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150929.1">
        <property type="nucleotide sequence ID" value="XM_037293276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBB1"/>
    <dbReference type="GeneID" id="59330763"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="163" mass="18027" checksum="D0AA13394091DCF7" modified="2022-01-19" version="1">MVYFFLGHYSSPGAPELPAFVNASKTLCPTVMEICAERSRGEIYGSTAAMQTSCSQQLSDGVGDRPKLADEDKAFWSLVEHPWLLLTQFIGTALLTHLICDIVRGRRALKVGRNGGWELTKVTQEQVERFKMEAETLVQDTKEALKELAAAVDKPHGKRGDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBB7</accession>
    <name>A0A8H6FBB7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151592.1">
        <property type="nucleotide sequence ID" value="XM_037292173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBB7"/>
    <dbReference type="GeneID" id="59329659"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000050">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="6">
        <location>
            <begin position="48"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="551"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="561"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="576"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="152"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="631" mass="71109" checksum="28ED8F1E1A6D4A99" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBX5</accession>
    <name>A0A8H6FBX5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_000805</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152103.1">
        <property type="nucleotide sequence ID" value="XM_037291741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBX5"/>
    <dbReference type="GeneID" id="59329223"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="9">
        <location>
            <begin position="296"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6222757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="461" mass="51249" checksum="4413CB46AAA3E35B" modified="2022-01-19" version="1">MAILPSLSTLLPPPRSDLKPLSGTTTLTLLPALAPPLTYYLALLLLPPFPNTSSEPTARVIPFLRNSLALLSGILFFRLPLKYHVPFSVGLTYQLALVGLYGGCRVLDAFFISPYLFQHIPRRVKYHHAPRTLAPSTPLGADAKSCSSLHFLTPGDGEAVTETATTDEGWPHGWKDRISWALELELSMRGAGFTWSSADVRHTKSTWRPSIRDRTHSILVHVLPILVVSWAVIRDIYSLNLANGEPFDDLPIGLQLVLTAALGAFLMSAFSLGHSTFAIMLAPLAPHPLSYFPPLYTARVWELTSVRDFWSYGWHRLFSRLFLVYGVWPGEWLERKLTGKPDEEAADMGKVLGGFVSSAFVHSFAAHTVMGGWEGAFGEAWFFAGNGVAVVVEEGVRMAVVRYRRWQKRQGAGDGKDEVGLERWYDGVIGRVWWVCVLLYMGRNFARGWVRAGLVREMAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC23</accession>
    <name>A0A8H6FC23_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151739.1">
        <property type="nucleotide sequence ID" value="XM_037292320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC23"/>
    <dbReference type="GeneID" id="59329806"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="248" mass="28110" checksum="B508A00B34985798" modified="2022-01-19" version="1">MYNLASQIRSPKLRTIRTDIDIYKKVDGEIKSEYIKMRKRAELQGIEQIVLQSRKLLTESRDEEADLEDKCLIQRLQKANHARRQLFECWRRSKQQSIQAASKAIETVPSSQHHDERLTRVPKHHIPSSVQLSEISRLLLSSVPALPKDYILRGNNKYTYSGTSPGLTVHGLSGEKTSRKLNAGLDRTSSMTFDPMSVHTMVVPNQMCYTIAGKSGHAMSNARISKVYGDVRSTLSMNTLSLLRTKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCV2</accession>
    <name>A0A8H6FCV2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152420.1">
        <property type="nucleotide sequence ID" value="XM_037290985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCV2"/>
    <dbReference type="GeneID" id="59328464"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="209" mass="22963" checksum="F6E309A122271AAC" modified="2022-01-19" version="1">MPPSLAHMESSVTLQGSEHEDDGEDETEGSKREESTPAGPVSQEDGRTPEALKEADSWGFVVYRTTFKQKLSRHVERGRDAIVDARGDPDGVIFDFIEDEPELDGATAEQVRLRHDPSYVSPAVDPEAAEDQVVPSERDDGLCGAVFLMIDRDAMESVIRHPARGNPYVVAVDAINNHYEEGVFEGTMKIHADDFLTELSAEYQGQCAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCW1</accession>
    <name>A0A8H6FCW1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000076</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152451.1">
        <property type="nucleotide sequence ID" value="XM_037291016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCW1"/>
    <dbReference type="GeneID" id="59328495"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11060">
        <property type="entry name" value="CYP57A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF229">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="4">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="555" mass="62833" checksum="A4BE848CC9D0E4FB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDI3</accession>
    <name>A0A8H6FDI3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADPH-dependent FMN reductase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010937</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153420.1">
        <property type="nucleotide sequence ID" value="XM_037301786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDI3"/>
    <dbReference type="GeneID" id="59339327"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050712">
        <property type="entry name" value="NAD(P)H-dep_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30543">
        <property type="entry name" value="CHROMATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30543:SF21">
        <property type="entry name" value="NAD(P)H-DEPENDENT FMN REDUCTASE LOT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADPH-dependent FMN reductase-like" evidence="2">
        <location>
            <begin position="8"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="217" mass="23933" checksum="B75B1EB909C3C999" modified="2022-01-19" version="1">MSSPTPPSIGVILCSSRQPRVCPQIAAFVTETIETRTSPSPPDPHAAPPPPPPPPAHLHTIDLAAWNLPLFDEPNIPSQITAPSGYSRPHTRAWSQEVRKHAAFVFVLPQYNWGYPAVVKNAIDYLFHEWRGKPAMIVSYGGHGGGKAAGQLRQVLQGVRMHVAETMPALAFPSERFVLRASKGEELPLRGDEGIWRSERAVVREAFEELMALVSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE17</accession>
    <name>A0A8H6FE17_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153782.1">
        <property type="nucleotide sequence ID" value="XM_037300965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE17"/>
    <dbReference type="GeneID" id="59338501"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="796"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="852"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="823"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="859"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="882" mass="98053" checksum="A97224CEA49DF33F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEN1</accession>
    <name>A0A8H6FEN1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BAG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010473</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154143.1">
        <property type="nucleotide sequence ID" value="XM_037301326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEN1"/>
    <dbReference type="GeneID" id="59338864"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="2">
        <location>
            <begin position="881"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="638"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="950"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="638"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="668"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="733"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="757"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="950"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="981"/>
            <end position="991"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="991" mass="111874" checksum="EA39F6012E408791" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FES8</accession>
    <name>A0A8H6FES8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010381</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154051.1">
        <property type="nucleotide sequence ID" value="XM_037301234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FES8"/>
    <dbReference type="GeneID" id="59338772"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03272">
        <property type="entry name" value="ABC_SMC3_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000424">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041741">
        <property type="entry name" value="SMC3_ABC_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="11">
        <location>
            <begin position="540"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="733"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="849"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1065"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1125"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="278"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="431"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="805"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="745"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="774"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="859"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6225184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1255" mass="143075" checksum="48224338FA30BB98" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF13</accession>
    <name>A0A8H6FF13_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_009468</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154177.1">
        <property type="nucleotide sequence ID" value="XM_037300329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF13"/>
    <dbReference type="GeneID" id="59337863"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="168"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6225468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="391" mass="42982" checksum="2823E139053CEA00" modified="2022-01-19" version="1">MEYLRDVLRTNRGVSLPLTALLALSTVIGIAVLFYLALFIVAPIPRAPLRSEKTYRTIEPGEYSGEAKPLPCWQDKYNAQGEAARRRGDSTETDLQVEEPEVFMSLVVPAFNEEERLGVMLTEAVEYLQHAYGNEAPKMQANGNRPKRKDATQAVSNGYAASNSGRLPGWEILVVSDGSTDKTIDTALQFARDLGGRSASCIRVISLHENRGKGGAVTHGMRHVRGQYAIFADADGASKFEDLGKLVRESKKIEDAEGRGVAVGSRAHLVGSEAVVKRSFLRNLLMHSFHLLLRLLTPPATAAIRDTQCGFKLFSRPSLPYIIPYMHSEGWIFDVEMLMLAESAGIPVAEVPIGWQEVKGSKLNVLWDSLGMAWGLAVLRAAWGLGVYRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF66</accession>
    <name>A0A8H6FF66_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF914-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009619</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC35F solute transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154328.1">
        <property type="nucleotide sequence ID" value="XM_037300480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF66"/>
    <dbReference type="GeneID" id="59338014"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009262">
        <property type="entry name" value="SLC35_F1/F2/F6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052221">
        <property type="entry name" value="SLC35F_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14233">
        <property type="entry name" value="DUF914-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14233:SF4">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06027">
        <property type="entry name" value="SLC35F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="42239" checksum="0F8FC82F52AF36E6" modified="2022-01-19" version="1">MHTHNGKSSAIDQVDPVTGDTKDLPSSSSSPVHAAPEVIGINDRAPDERTGENADEIQASQGGWFAYLRTRNFYLVLLLGQVLALCITATNTFSSLLAAQPFSIPAFQTLWNYILLTLVYGSYTIYRYGWKGWIKLMLKDGWKYIILAFLDVEGNYFTVLAYRYTSLLAQVIIGILICIGGVGILFGSDHITQGSGFGGVTGGNQLKGDLFALVGATFYGMSNVAEEFLVSKRPLYEVVGQLGFWGMFIDGAMTGIFDRPSYRQSVWNDGKVGGYLTGYTLCLLLFYSGAPIMFRLASAAFFNISLLTGNFWGVVIGVKVFHERIHWMYPIAFVCIIGGLIIYFVGKSILGEALKPWLGKDQERGVNGLGTARRRVERGDELVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFW6</accession>
    <name>A0A8H6FFW6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009234</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154921.1">
        <property type="nucleotide sequence ID" value="XM_037300095.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337629"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04043">
        <property type="entry name" value="C2_Munc13_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052811">
        <property type="entry name" value="Glucose_resp_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010439">
        <property type="entry name" value="MUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47263">
        <property type="entry name" value="ADENYLATE CYCLASE ACTIVATION PROTEIN GIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47263:SF1">
        <property type="entry name" value="C2 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_7G02350)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06292">
        <property type="entry name" value="MUN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD1" evidence="3">
        <location>
            <begin position="699"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="905"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="4">
        <location>
            <begin position="1117"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1368" mass="156728" checksum="D101D55D7A24CDDB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG33</accession>
    <name>A0A8H6FG33_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009429</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155116.1">
        <property type="nucleotide sequence ID" value="XM_037300290.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337824"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056669">
        <property type="entry name" value="DUF7767"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057940">
        <property type="entry name" value="Tri-helical_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF147">
        <property type="entry name" value="SEL1L ADAPTOR SUBUNIT OF ERAD E3 UBIQUITIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24962">
        <property type="entry name" value="DUF7767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24465">
        <property type="entry name" value="Tri-helical"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1394"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="domain" description="Tri-helical" evidence="4">
        <location>
            <begin position="242"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Tri-helical" evidence="4">
        <location>
            <begin position="337"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7767" evidence="5">
        <location>
            <begin position="551"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="415"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="657"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1421"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1474" mass="164343" checksum="0B7108E7723B346C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGE3</accession>
    <name>A0A8H6FGE3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008758</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155623.1">
        <property type="nucleotide sequence ID" value="XM_037299622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGE3"/>
    <dbReference type="GeneID" id="59337153"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="139" mass="15834" checksum="E304E2B94CDC4441" modified="2022-01-19" version="1">MTWKPTTEWEGKLKDITWSFGNEDVRFRHEQWRKVFEEQLKSTPFSIQAAEPLFSLPLGEESVEFTQWLSSDAIWDRYHTLSQFAVLEGQELADTKRRVLEATGAADVGKNENGELELHGRTYFAWSTAVPGAPLKSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHF7</accession>
    <name>A0A8H6FHF7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007657</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155863.1">
        <property type="nucleotide sequence ID" value="XM_037298527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHF7"/>
    <dbReference type="GeneID" id="59336054"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="348" mass="38123" checksum="6E97B270295FABF7" modified="2022-01-19" version="1">MAHLRDVGPRLLPGTKEPIKTVYTGVPAIDHQLTVLALFFWENVDGSNPAGSLFCFHFATQVACGWGLLMIEGLRHGNRWTIISFIGTWGIFVQNAAYAVVIPYWCIAYLSTSRIVSSRRVSEYLVDVPNLAGIAISMVLGYVLPTILMSLPAPSIIDYDLKQWLMTFWQFFPVWVSVVQGVVPYLLPKPGEASRVSDIHMLMSMRILYAGLITTAGIGQASTMTFLATSKFFPELFTPEFVGVFNPSNVFLPATISPSTKVPSIGAGAFLLLQYDHLIGSTSMALWSTVLFVNTYRNGATYQSVGLMIVGGIIMMALTGPLGYATACIWARDELIIAEAEADGKKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI39</accession>
    <name>A0A8H6FI39_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156575.1">
        <property type="nucleotide sequence ID" value="XM_037297940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI39"/>
    <dbReference type="GeneID" id="59335445"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="50487" checksum="49AF6B9FDD2289DD" modified="2022-01-19" version="1">MDIGYNAARILYLPFELWGSVCQWLKKDDIRSTRLACRLFNEVASPFLLQRVWISSSSEDQKTLTAISLHQVFSKYVKEIYYDSTIYDPDLLTIFNDDRSYGKYIRRFLKNRSRTIWPKNALRYAISQYKVRCKEQIELQAYEGAHLTRSSDAPPNFGSLLTAAKGSEEVPHRLDVDTFDRFWYRGFYVLTQAASMLNLKQLRSFVTDSAKQGISYTVLDMASTELMHTCNAFRHLATISLQIDTQNGNSQWNWIKFLGGGNVALMLTAAQGLKHLDLGFDMPYTEIRKLAKLLGHLAWPCLQHVGLSNMILEEDELVAFLKRHISTLESISLDYMILFKHHPQDRIFGDWTPKSWKGAFRSMSVLALNNLCISIPLSDGEPCSMRGYWHGDDPAKVNRFLASGGYTGFAENELQYDQEQQRQWLQAQWEYENLDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI52</accession>
    <name>A0A8H6FI52_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156612.1">
        <property type="nucleotide sequence ID" value="XM_037297977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI52"/>
    <dbReference type="GeneID" id="59335483"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053060">
        <property type="entry name" value="Cytokinesis_Signaling_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36419">
        <property type="entry name" value="ARRESTIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36419:SF1">
        <property type="entry name" value="RHO1 GEF LOCALIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="1">
        <location>
            <begin position="185"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="401" mass="44630" checksum="B0B82579317E9057" modified="2022-01-19" version="1">MTAFVRVCGPPNSAYLVGYPGISATLPRVEGKVEIRPDKGYSAPVAISLVTIYLQRRETIHPSADSVTKKHLAAPRKELVDIVGKEMLLFRCPAGKESENVYSMDLPFVLFIPFGRGGEETARRVPPASLQLGSRTAETYYEMVVMVQQGHTEQRKYAFPVPISRYDTLSTFGMYNRPESAERITDHLVTLGISLPRWSYGPLDPVSVYIKLSPNPDWLNKAKRVTINKITIGIDEEIIYNHEGDEPQRKVKTLAKQTQTVGVKMPEAGYFTNLGLVFPAKDLRDNDGVIPRGKQAFPTYSVSGFTTTGSLYKIEYYLTVKAHMTSAKDILLRQPIVVCPLDHTACKEEMEAIEQAAKDAGNIRPDNPMLPAPTIIRVNDYNALHTLGMTMVGSTRKPLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIC4</accession>
    <name>A0A8H6FIC4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156699.1">
        <property type="nucleotide sequence ID" value="XM_037298064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIC4"/>
    <dbReference type="GeneID" id="59335571"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="245" mass="28108" checksum="0C0665A6C9854986" modified="2022-01-19" version="1">MCIRHEADGRLVDQTDMDPQERLPRIERLSLECYRFDSDMRGLQFHLSSFLCKRHDFEELELESLGVSRAIIGVIVKRNGSTIRKLHIHNLEQAVLLWRTDQPVPQCESSIPSHVILSIWSYCPHLRSPELDLTKTDITGHSDINTQLSALAIDSNNPYMAVVRVRDLACEIWSYFPETFRVVTRSLARNGTQLGGNCSWLATRNFDDLDIDGWDVEIVDEVAAAAERKLRVWARAVEVPFEPTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FID9</accession>
    <name>A0A8H6FID9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007214</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156742.1">
        <property type="nucleotide sequence ID" value="XM_037298107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FID9"/>
    <dbReference type="GeneID" id="59335614"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF14">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="4">
        <location>
            <begin position="326"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="807"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="841"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="875"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="909"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="1002"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="1035"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1112"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1117"/>
            <end position="1129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1143" mass="127331" checksum="6F6E4BB674D51E78" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIM6</accession>
    <name>A0A8H6FIM6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phenylalanine ammonia-lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PAL/histidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156867.1">
        <property type="nucleotide sequence ID" value="XM_037298232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIM6"/>
    <dbReference type="GeneID" id="59335740"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="653" mass="69818" checksum="CB729E4C74F6A2E6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIT8</accession>
    <name>A0A8H6FIT8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156973.1">
        <property type="nucleotide sequence ID" value="XM_037298338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIT8"/>
    <dbReference type="GeneID" id="59335846"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046784">
        <property type="entry name" value="Eap1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20566">
        <property type="entry name" value="Eap1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="638"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="679"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="704"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="728" mass="80486" checksum="B8C2356929C57F5E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIV5</accession>
    <name>A0A8H6FIV5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157017.1">
        <property type="nucleotide sequence ID" value="XM_037298382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIV5"/>
    <dbReference type="GeneID" id="59335890"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="168" mass="19196" checksum="4E9B46899BFD5BA5" modified="2022-01-19" version="1">MSDQLLSHPQKLYSTIETTLYDDVSGPRGYSEPILTPFFIPQSESAHGCLRVNVLAGTDLETTLGVGQTPFLYAIGLLHKAKRLGTIVGSQEGLLFTIFDIYSRPALYDTINPLDPLLFARATASLDEQRKFYNRVWRLYMHSDRYILVFAIGQRRKLRLVMPDFIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ01</accession>
    <name>A0A8H6FJ01_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DUF221-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_007518</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157044.1">
        <property type="nucleotide sequence ID" value="XM_037298409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ01"/>
    <dbReference type="GeneID" id="59335917"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF26">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G10920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="461"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="510"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="558"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="605"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="629"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="678"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="12">
        <location>
            <begin position="33"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="13">
        <location>
            <begin position="205"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="10">
        <location>
            <begin position="410"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="11">
        <location>
            <begin position="791"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="744"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="758"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6229402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="891" mass="99346" checksum="FE3FA3C33056EC46" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK31</accession>
    <name>A0A8H6FK31_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Valine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="4">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Valyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_004342</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157261.1">
        <property type="nucleotide sequence ID" value="XM_037295260.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332750"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000009">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000020">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000078">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000005">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.40">
        <property type="entry name" value="Autophagy protein 6/Beclin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038274">
        <property type="entry name" value="Atg6/Beclin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041691">
        <property type="entry name" value="Atg6/beclin_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040455">
        <property type="entry name" value="Atg6_BARA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004349">
        <property type="entry name" value="PRK05729.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF109">
        <property type="entry name" value="VALINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04111">
        <property type="entry name" value="APG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17675">
        <property type="entry name" value="APG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atg6/beclin coiled-coil" evidence="19">
        <location>
            <begin position="167"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Atg6 BARA" evidence="17">
        <location>
            <begin position="298"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="16">
        <location>
            <begin position="665"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="18">
        <location>
            <begin position="1333"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="109"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="374"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="562"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1587"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="579"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6230004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1617" mass="181052" checksum="AD821E8983C0CEC2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKJ0</accession>
    <name>A0A8H6FKJ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4</fullName>
            <ecNumber evidence="6">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Carbon catabolite repressor protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Cytoplasmic deadenylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Glucose-repressible alcohol dehydrogenase transcriptional effector</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">HO133_004517</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Acts as a catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. Ccr4 has 3'-5' RNase activity with a strong preference for polyadenylated substrates and also low exonuclease activity towards single-stranded DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157435.1">
        <property type="nucleotide sequence ID" value="XM_037295434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKJ0"/>
    <dbReference type="GeneID" id="59332925"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09097">
        <property type="entry name" value="Deadenylase_CCR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000037">
        <property type="entry name" value="Glucose-repressible alcohol dehydrogenase transcriptional effector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000447">
        <property type="entry name" value="Glucose-repressible alcohol dehydrogenase transcriptional effector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF100">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="25">
        <location>
            <begin position="471"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="68"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="170"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="672"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="214"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAF6230178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="847" mass="95501" checksum="FDA07C57A3ACA604" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKR5</accession>
    <name>A0A8H6FKR5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004647</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157564.1">
        <property type="nucleotide sequence ID" value="XM_037295563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKR5"/>
    <dbReference type="GeneID" id="59333054"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="689"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="Septin-type G" evidence="4">
        <location>
            <begin position="225"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="137"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6230307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="732" mass="79849" checksum="9C1500097F667D77" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY85</accession>
    <name>A0A8H6KY85_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GED domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156633.1">
        <property type="nucleotide sequence ID" value="XM_037297998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY85"/>
    <dbReference type="GeneID" id="59335505"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF215">
        <property type="entry name" value="DYNAMIN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GED" evidence="1">
        <location>
            <begin position="241"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="329" mass="36410" checksum="8ECA932C3B73C572" modified="2022-01-19" version="1">MGAAHVSPRRLSSVEPRIRVSQSQLSRRVRRIQKLKERLQAFKIHHMTSLSGEELLQVLSKACIVMSIPALGHSLAPGRSSFSDDTMKIELSGPSRQHLSVVDVPGLFRTPTEGVSTKEDMILVRRRVKSYIKDSWTIILAVIPAPVDIATQEILSLAEEADPLGQRTLGVLTKPDLLDKGGEELVMDLVRGTKNKLNLGYCMVRIRGHQDKAPSGTDRHQTFKMNAVVDSIIMGNIQHTVEDIHAILKSYYRVARKRFVDVVCMQAADHLFVTGPGSPLTPFTPSFVHHLSLDSLILVAGEGVISKRTRSGLIHGIECLRAGKRMLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZZ8</accession>
    <name>A0A8H6KZZ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004802</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157716.1">
        <property type="nucleotide sequence ID" value="XM_037295715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZZ8"/>
    <dbReference type="GeneID" id="59333208"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6230459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="30289" checksum="954689B6E3203000" modified="2022-01-19" version="1">MKLARTADLAGMEAMFEAGQASPTDVAARTNSVQLIEYLLSKRLNASTTDDEGETPLHLAIAHSSDYNVSRLLIDKGADINSSSIDGGSPLHTFFNETVSRVILCHGKSMEANTKDSRGRTPLHYLAWSSRSTVKDINACPVDESCFAAQDEEGRTPLHFAARRGNVELAKYMINLLPSPSINQTDRNGQTAFHCATESKRVEIIDVMKDGGLDVRGRDHRRRSPLHHAALHGNLDAVRKVVALAGRQELEQRDLDGRTPFQLAERYGGGREPEAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6R3</accession>
    <name>A0A8H6C6R3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006394</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147417.1">
        <property type="nucleotide sequence ID" value="XM_037297292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6R3"/>
    <dbReference type="GeneID" id="59334795"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="3">
        <location>
            <begin position="325"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="714"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1013"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1015"/>
            <end position="1028"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1036" mass="116674" checksum="CEC1AE786399574A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6T8</accession>
    <name>A0A8H6C6T8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147380.1">
        <property type="nucleotide sequence ID" value="XM_037297255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6T8"/>
    <dbReference type="GeneID" id="59334758"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="15"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="548" mass="60452" checksum="82F5013CD7863C27" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Z2</accession>
    <name>A0A8H6C6Z2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CoA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006593</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147201.1">
        <property type="nucleotide sequence ID" value="XM_037297491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Z2"/>
    <dbReference type="GeneID" id="59334994"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000017">
        <property type="entry name" value="Succinyl-CoA synthetase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000340">
        <property type="entry name" value="Succinyl-CoA synthetase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF6">
        <property type="entry name" value="SYNTHETASE SUBUNIT ALPHA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G10830)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="3">
        <location>
            <begin position="51"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="709" mass="76531" checksum="A62F1DEC9786F36F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C735</accession>
    <name>A0A8H6C735_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147597.1">
        <property type="nucleotide sequence ID" value="XM_037297023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C735"/>
    <dbReference type="GeneID" id="59334525"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="381" mass="41722" checksum="596043C324160E88" modified="2022-01-19" version="1">MDFLKKAKQTMKELEGDLNKATAQLGFGDKSQNGAQVPVAVEPASSNSTPATSTINTPSTSVAPSTAGGDAPKTKLPLAIRKNVRDEFETKIPELEANLSSVLGQPWKISFDPGHLYTLAEDRFSKEKPGQMFTQYCQGATRGISDYIEQFGADGKSELNTLATSHTMTLEQTTNPKISYSGCEISGGMLRLVFRDKYLGSNINTVCAELATAVNNAGMSSNPEGGAALDFNAKNGIKKDYEPEIGNVKAKIQSILGLPMLELHPNFERNFAAIAAYTASGKQPTTYPREWQKKLGFHTFKYFESLVSSLEEQGFAKDDMLQEGYQETADKNEIGLRIVDKLQKGTYNECVFENGVMYIQTTPQYWTSNIRDAGAKIVDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7C8</accession>
    <name>A0A8H6C7C8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147728.1">
        <property type="nucleotide sequence ID" value="XM_037296547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7C8"/>
    <dbReference type="GeneID" id="59334044"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054208">
        <property type="entry name" value="DUF6914"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21858">
        <property type="entry name" value="DUF6914"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="356" mass="39347" checksum="8C7F16B0DCCC19C1" modified="2022-01-19" version="1">MSKDRNTTPTFDARTTANPDSPFVGIPLLFIGIYSNTDVNEEDYRWSFIVYPAKKEGASEEQEYLLLWRLGHGRGNRCRFEAHQGRLRDQENMLGWLVIAEVKNLKELDDTLLSSSPSTTVPENRKSRSLFWVKAMLERLVDAECLKGKAKAFESIETEGREIAQRHTQLGKTMYVPQDNALLDRRPSQGAETGKEAETVGRGKEKNAILQSQQIHSAPSTDDRTSHVRSNHPATSSTGKTVTNLVPEYNTDSDSDAEDNSKDAVEVHRPSSSVGQPSVPSPSTAPPSTPPTSMGPPSKPASSMAPPKLPVSATRGKRRHPAGRDAGTEAPAPGRKYFTMDDLPLVRYDKKGHRVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C819</accession>
    <name>A0A8H6C819_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005958</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148042.1">
        <property type="nucleotide sequence ID" value="XM_037296861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C819"/>
    <dbReference type="GeneID" id="59334363"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="165" mass="18492" checksum="1D225F560DE567F5" modified="2022-01-19" version="1">MSHPTPLTSLQEQMTQMMEMIRSMPAQQSTQSATQPATQQVTQPPHQSTQSATQLATQSDTLQEEEQEEELQQANAENTAEKPSGNKATIVKNTAHVSTIPRQVITQQMENTTTIWPAISACLGNITLWYITAMSTQEKDLLRRSQPIGPLQARVTYRTMHIWTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8I0</accession>
    <name>A0A8H6C8I0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cAMP-independent regulatory protein pac2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147996.1">
        <property type="nucleotide sequence ID" value="XM_037296815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8I0"/>
    <dbReference type="GeneID" id="59334316"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018608">
        <property type="entry name" value="Gti1/Pac2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027:SF1">
        <property type="entry name" value="CAMP INDEPENDENT REGULATORY PROTEIN (AFU_ORTHOLOGUE AFUA_3G09640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09729">
        <property type="entry name" value="Gti1_Pac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="57962" checksum="BBC562C1C686720B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8W1</accession>
    <name>A0A8H6C8W1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004917</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148527.1">
        <property type="nucleotide sequence ID" value="XM_037295829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8W1"/>
    <dbReference type="GeneID" id="59333323"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF34">
        <property type="entry name" value="FAD BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G02770)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="92"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="523" mass="56048" checksum="0DE6ECA7A06FC23F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9C1</accession>
    <name>A0A8H6C9C1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148489.1">
        <property type="nucleotide sequence ID" value="XM_037296507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9C1"/>
    <dbReference type="GeneID" id="59334004"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="80" mass="10392" checksum="81978213E8051A78" modified="2022-01-19" version="1">MDEEVDEGRRREKEKEKEKEKEKEKEKGRRRRRRRRRRRRREKEKEEKEKEKEKEKEKEKEKEEEEEEEEEEEVFEGWGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9T9</accession>
    <name>A0A8H6C9T9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149110.1">
        <property type="nucleotide sequence ID" value="XM_037295063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9T9"/>
    <dbReference type="GeneID" id="59332553"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="29489" checksum="D99700474567F1DF" modified="2022-01-19" version="1">MSNILRRVLSFGSPSRATSTSTLEDEDTMFDVQEPELEETDPIPNFAEEGITDHTEVLIELMQGPSVSVHVGKATKPITISKRLLMYHSPVAARLLAKQTTPDTLALPGHDPFAFTHIYRWMHQDKLDILPYCTRTGTSPTAIQDACTLLCRIFAAARYLDITPIHGLVFQELRAAFAITRDAGKSTPVAPGTVLEVWRDGGEGCGLWKVVLEEMCVAFTRKPMAVFAVYDECFERIEPFRLAVGEAMTGRIFWGSEVGGVKVEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Z7</accession>
    <name>A0A8H6C9Z7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Argininosuccinate synthase</fullName>
            <ecNumber evidence="3">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_005225</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148834.1">
        <property type="nucleotide sequence ID" value="XM_037296136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Z7"/>
    <dbReference type="GeneID" id="59333631"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000019">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1260.10:FF:000003">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048268">
        <property type="entry name" value="Arginosuc_syn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048267">
        <property type="entry name" value="Arginosuc_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001770">
        <property type="entry name" value="PRK00509.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20979">
        <property type="entry name" value="Arginosuc_syn_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="Argininosuccinate synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginosuccinate synthase-like N-terminal" evidence="12">
        <location>
            <begin position="6"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Arginosuccinate synthase C-terminal" evidence="13">
        <location>
            <begin position="179"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6219399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="415" mass="46852" checksum="FEFA47A290FD9E9D" modified="2022-01-19" version="1">MAPKQKICLAYSGGLDTSCILKYLLEAQYTVVCFLGNVGQEEDWAAVREKAMKIGAEKLIIRDVRKEFIEEFCFRAIQCNAQYEGRYLLGTSLARPVISRAQIQVAKEEGCDIVSHGCTGKGNDQVRFELAFHTLAPGIKIIAPWRDPEFFERFQGRNDLLDYAEKMGIPVTSTKAKPYSMDDNIAHCSYESGQLEDPSRPPPDGMWTRTDDPLTAPNNPQEVTVRFEKGVPVKVSSEGKEWTDSLDLFNALNTIGKKHGIGRIDIVENRFIGIKSRGCYDSPAMTILRWAHLDLEGLVLDGRVRSLRDQFVTHTWTEILYNGLYFSPEREFVENSILFSQQRVNGEVKVRLYKGNCDIMGRSSETEKLYDESESSMDVLNFDPVDTTGFININAIRLKKYGLAKLEEGNDLAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA71</accession>
    <name>A0A8H6CA71_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_003592</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ATG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149202.1">
        <property type="nucleotide sequence ID" value="XM_037294514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA71"/>
    <dbReference type="GeneID" id="59332003"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16128">
        <property type="entry name" value="Ubl_ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000010">
        <property type="entry name" value="Autophagy-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="9">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6219767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="119" mass="13793" checksum="ACE1E7907255E147" modified="2022-01-19" version="1">MRSKFKDEHPFEKRKAEAERIRQKYADRIPVICEKVEKSDIATIDKKKYLVPADLTVGQFVYVIRKRIKLSPEKAIFIFVDEVLPPTAALMSSIYEEHKDEDGFLYITYSGENTFGSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAZ9</accession>
    <name>A0A8H6CAZ9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149648.1">
        <property type="nucleotide sequence ID" value="XM_037294267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAZ9"/>
    <dbReference type="GeneID" id="59331755"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="179" mass="19938" checksum="162BD58A7CCB3342" modified="2022-01-19" version="1">MPHPTPLASLQEQMTQMMEMMRVMSTRLEALEARSRPTQPATQPTTHSVAQSGISQEEDIAEKSAETPVQEKSLQQANAENTAENTAEKPSDSKAPSTKNTAQLSKIPRHLSTIPRQIATQQAEDDTTIWPAISACLGNITLWYITAMSTQEKDLLRRSQPIGPLQARVTYRTMHIWTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBD6</accession>
    <name>A0A8H6CBD6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SRR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003464</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149767.1">
        <property type="nucleotide sequence ID" value="XM_037294386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBD6"/>
    <dbReference type="GeneID" id="59331875"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRR1-like" evidence="2">
        <location>
            <begin position="67"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="274" mass="30673" checksum="95997376524310C1" modified="2022-01-19" version="1">MENKTKVAKTRAFPTPRPPAAAPKAPRPAAPRHKPLTPKEVKAGIETSTQKFRNSTRFHSLTAFFEQGQKTNITQCICLGLGPFMNFESKSARGRKRNGDFNTSLHQPAMLESLPNGLTKDEETKIYFQDPVFTRSERDHLQSRDLKAKNISRALTDTFPALYIGTDPDAAIADIGKDNAKKIPGGKEKYIRPLQEFGRDSAETLRLPIFAHGVRDGHDVWAEASVHWRVPDGGGEKVGKRLGGGGMARARVEIGARHYHLVRRERKMERERNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBQ8</accession>
    <name>A0A8H6CBQ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DEAD/DEAH box helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003068</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150070.1">
        <property type="nucleotide sequence ID" value="XM_037293992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBQ8"/>
    <dbReference type="GeneID" id="59331480"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036121">
        <property type="term" value="F:double-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061749">
        <property type="term" value="F:forked DNA-dependent helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032042">
        <property type="term" value="P:mitochondrial DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18799">
        <property type="entry name" value="SF2_C_EcoAI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050742">
        <property type="entry name" value="Helicase_Restrict-Modif_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE IRC3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME ECOKI R PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="37"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="261"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="619"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="619"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="647" mass="71879" checksum="3E3B266DB2F3F5E0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBR3</accession>
    <name>A0A8H6CBR3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149801.1">
        <property type="nucleotide sequence ID" value="XM_037294420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBR3"/>
    <dbReference type="GeneID" id="59331909"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF2">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="37504" checksum="FC171A568FDC9E11" modified="2022-01-19" version="1">MAHMLDGPVPDGARSVSWPWYHHVLTDELTVDARALFEQYAKVPPKDVENHIYRIRDKAWGIFPWPCIGEFWFLSFGLAKHPLYHNLVLPRLKTGDSLLDLGSCMGQDLRKLVFDGAPSEKIYASDVFPQYEGLSYELWRDRDTFPHHFLADDVLADNEDFMKGALMTKLGPGQTDIIAINMFLHLFDYENQLKVATRIIRLLSHRPGSLILGSQAGVRPPMEQPLKPPFDKTEDGETRTIYRHSPDSFIDLWKRAGSFAGVPIRVWADFQIMEKKSEQYPAGVDTGLEAVASKKPFTADDLFSTSSAKRVLFSTEPEARRLYFSIIRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBU0</accession>
    <name>A0A8H6CBU0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150115.1">
        <property type="nucleotide sequence ID" value="XM_037294037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBU0"/>
    <dbReference type="GeneID" id="59331525"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="354" mass="40125" checksum="2116E609E335A410" modified="2022-01-19" version="1">MTSLPGMALPIITIFQDATVGLISLMFKPYLPFDLAGVNSMVRIAVALNDKENGPTQADGRAPSLVAFNEKEWYIGASEWVANDHVKSGSYVDVVVHQEDDGAGQEPTYMQVLGHDDAVCVAYIGQTWPDGQQRGWLGDVGRACGKRWFFSNVKVGEQKYMPDCTWLDRDHAKDGTDALENIEAAAMQIHMQDFTNLTEPYPTDPSYYCTYPATLFVRDYDYEFRLEWFEHKIPSRSRIAFWKDHGFRSKRRSAHPHRRRPQPSKRSLALRSKLVSSTHEKHSAKALCGSDTSHGPDFVSHSEGLFCDMGTKRVWPLCKTTEEVEVGCYDWESHGLVMREGKEGRDYADVEEWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC00</accession>
    <name>A0A8H6CC00_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149899.1">
        <property type="nucleotide sequence ID" value="XM_037293821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC00"/>
    <dbReference type="GeneID" id="59331308"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="558" mass="63401" checksum="2F52C91339EC2365" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC26</accession>
    <name>A0A8H6CC26_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002979</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149981.1">
        <property type="nucleotide sequence ID" value="XM_037293903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC26"/>
    <dbReference type="GeneID" id="59331391"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000036">
        <property type="entry name" value="Putative sulfate transporter YPR003C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="594"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="652"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="802" mass="86513" checksum="2B7272FA0E0C130F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC80</accession>
    <name>A0A8H6CC80_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003017</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150019.1">
        <property type="nucleotide sequence ID" value="XM_037293941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC80"/>
    <dbReference type="GeneID" id="59331429"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1900:FF:000013">
        <property type="entry name" value="Spindle pole body component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF70">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="7">
        <location>
            <begin position="177"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="6">
        <location>
            <begin position="577"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="934" mass="105249" checksum="A2C4E6036F5BD9D8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC88</accession>
    <name>A0A8H6CC88_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003168</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150170.1">
        <property type="nucleotide sequence ID" value="XM_037294092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC88"/>
    <dbReference type="GeneID" id="59331580"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052649">
        <property type="entry name" value="NCE102-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28165:SF2">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28165">
        <property type="entry name" value="NON-CLASSICAL EXPORT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="6">
        <location>
            <begin position="5"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="163" mass="16519" checksum="56F392D076747995" modified="2022-01-19" version="1">MSSIPNMALRGLEIFFGILVLGLSGHLVASQAYGGAPSATNYNVFLGIWLLVVAAVGLAASFVSALGGVVMAALDALSVLFAFAGGVAYAAKLGAHSCSRASYTAHNVITNGGVDHAGNTAPGVNLGNRCREAQADTAFIWFAFAAFAASAALSLLAWRRGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDD2</accession>
    <name>A0A8H6CDD2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150641.1">
        <property type="nucleotide sequence ID" value="XM_037292988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDD2"/>
    <dbReference type="GeneID" id="59330475"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43669">
        <property type="entry name" value="5-KETO-D-GLUCONATE 5-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43669:SF4">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="232" mass="24520" checksum="18E356A599E9DE47" modified="2022-01-19" version="1">MPPPVILLLGAGANIGHGIAQRFSSSGYKVAIASRSGKSAQGTDYLSVKADLTQPQDVKSVFDTVKSEMGIPSVVVYIAYGLTMAAESDPLSPSLEKLTSDLAINTVSAFAAAQEAVKGFEELGSSAPKTFIQIGNMLNKMVYPRLITMGIGKQAAAHTIACCAEAYGGKGWHFYYVDERTENGQPAMSAIDGPAHGEFCFKLAQGKGQGPWDATFVKGVGYKDFESVTTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDH3</accession>
    <name>A0A8H6CDH3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150892.1">
        <property type="nucleotide sequence ID" value="XM_037293239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDH3"/>
    <dbReference type="GeneID" id="59330726"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="419" mass="45918" checksum="5B9382DAD1B8844C" modified="2022-01-19" version="1">MGAASDARAPLESTTNEARTPTPSPPSGTAKTGPILRRKSATQALGAYLDSSESYPSPHNSIYEAPFATSQEHLDSSARNDWDNAGLGIINHLTMRSNHDESPAWLNITLDASGSPVPASFLRSTETASPEKLMHTPHVLYPITEQSSLATLRPLSIVAHSSAATLRARASGLKRKSFSTSDLPPSPNPTFQQRSRPSSPVLTPIQPPHPPPTRSPTPPGLPSFNKPEASNYRLPPPPARFRDKFRSPTAAEREWLKQTVGLPKGVVMRGEDGVLVRGKFTPIRSGHLPPQRQVHGVYGMPTTGSGQGQRTMTGVLTDDQPPRVVRFEDRDRTANRNIKGREGHTEHGAKPKKKTEREERAKKWLKNAYWLIFCCGLCEEWDLAADSHQHGVQNIVVRGGRGRHGGCLGRLYPGELRNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDQ0</accession>
    <name>A0A8H6CDQ0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002433</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151012.1">
        <property type="nucleotide sequence ID" value="XM_037293359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDQ0"/>
    <dbReference type="GeneID" id="59330846"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058846">
        <property type="entry name" value="PAS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF30">
        <property type="entry name" value="TWO-COMPONENT SYSTEM RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26131">
        <property type="entry name" value="PAS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="520"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="678"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="1025"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="131"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="1084"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1158" mass="128950" checksum="65D861BFA331AFD8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEA6</accession>
    <name>A0A8H6CEA6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001906</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151373.1">
        <property type="nucleotide sequence ID" value="XM_037292834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEA6"/>
    <dbReference type="GeneID" id="59330320"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35041:SF3">
        <property type="entry name" value="FORMYLMETHIONINE DEFORMYLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35041">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="578" mass="64242" checksum="1BEC3DFD44D3C285" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEF1</accession>
    <name>A0A8H6CEF1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151387.1">
        <property type="nucleotide sequence ID" value="XM_037292848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEF1"/>
    <dbReference type="GeneID" id="59330334"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="152" mass="18178" checksum="4C7F3ED734FE3B09" modified="2022-01-19" version="1">MIWSNAMKKNTRNNNMIDLNFGCNHMTCVRKAEFCYVGDKVWKNCNCPQFHDDYHEEFRDDADGLADRVAVGYLPGYRRRTVFRAERAVERYAKHLRQTWGRPDPRSREEQFQEVRDEVENGCDNGYWQLIRMIKDEPGHCKLCRREEPQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEV7</accession>
    <name>A0A8H6CEV7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151599.1">
        <property type="nucleotide sequence ID" value="XM_037292180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEV7"/>
    <dbReference type="GeneID" id="59329666"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044669">
        <property type="entry name" value="YneE/VCCN1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281:SF16">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281">
        <property type="entry name" value="UPF0187 PROTEIN YNEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25539">
        <property type="entry name" value="Bestrophin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6222164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="369" mass="41497" checksum="338ADBD7C5380ED6" modified="2022-01-19" version="1">MVNHINGNDEGRHHQRQPTIPFARSTRQKPRRWPLALRFIKGAIHGAIILPVALHGLFAALVVFLDQHLDGRLGLPASIIPSLSIVVGLMLVFRNQTCYNRFWDGRNYLTVIITSVRNLTRSFLACSAITSNTNQTLADRADTERVVRILVAILYATKNHLRAEWGAEIIPGTAISVNTGSATHIPEYSELLPQGLTGLDGRGVGLPLQLTFFVEQYIKKFFDKGMFHGPQASQMQVQLNTLTDAYGRMETIRLTSIPVAHLIHQKQVLALFGCVLPFALVEDMSWWAVPIVVLVMFTLYGIDGIGMQLEDPFGYDRNDIKMDAIVEDSRSEIMVLLEEWKRVSQTGREMFMGKQGLQRRSFRDGIQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG63</accession>
    <name>A0A8H6CG63_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHCH domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152278.1">
        <property type="nucleotide sequence ID" value="XM_037291916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG63"/>
    <dbReference type="GeneID" id="59329401"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF2">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="15635" checksum="9329AD5B295EDEAA" modified="2022-01-19" version="1">MPRSRGGSGGRGRPSAPSRPAAAPARPAATPSQQTSRGASTAAHPPAHAQQSGAPTQQGKSPGLFGQMASTAAGVAVGSSIGHAIGGMFGGSSAPTEQQQADNAVASQANESSSQSIAWGPRSCETDAKSFTKCLDENQGNMQICGWYLDQLVIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG99</accession>
    <name>A0A8H6CG99_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Alpha box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000963</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MATALPHA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152258.1">
        <property type="nucleotide sequence ID" value="XM_037291896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG99"/>
    <dbReference type="GeneID" id="59329381"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045895">
        <property type="term" value="P:positive regulation of mating-type specific transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006856">
        <property type="entry name" value="MATalpha_HMGbox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04769">
        <property type="entry name" value="MATalpha_HMGbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51325">
        <property type="entry name" value="ALPHA_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Alpha box" evidence="7">
        <location>
            <begin position="56"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="254"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="404" mass="43745" checksum="71E5A4A7F9262851" modified="2022-01-19" version="1">MSLTPAMKAALRKMTPDQIAAALQDAGQPRSTLKKERTAHGKIQKKDLVLVAAVTKAIRPLNSWIAYRSYYSTIFLSFQQKEISGFLTILWQNDPFKAKWSILAKSYSVIRDSQGKANAPLEQFLAINGPLIGVIEPTQYLEALSWEVAVDEDGQTVMRRNGNSIDEQLFTSNVSVNDVIRNSYDEGYFTGDLSKVLLANNEAAMTMAVSVQPTSNAHQSTTGHLESHDADFAATENFNGSSKEKKQVPTTVVIDDDGSNSAMATTANEAGPEESMAEDNDDIPGDSTALATENALPTGETSAVMTATVTSPVFDPHNLGPGSFLNIDAGMTEIQSHKNASANDPDVSSLSASAFHLDGEYPFNTEFDPDFSGATFNPFMGNQFNVFDMSDASWDDFIDFDASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGF0</accession>
    <name>A0A8H6CGF0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MYB transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000946</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152241.1">
        <property type="nucleotide sequence ID" value="XM_037291879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGF0"/>
    <dbReference type="GeneID" id="59329364"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052450">
        <property type="entry name" value="TRBD-Containing_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46734:SF1">
        <property type="entry name" value="TELOMERIC REPEAT-BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46734">
        <property type="entry name" value="TELOMERIC REPEAT-BINDING FACTOR 1 TERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="206"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="3">
        <location>
            <begin position="206"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="352"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="355"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="369"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="52254" checksum="30A8AD807F3BDF8D" modified="2022-01-19" version="1">MFDDMFDDFQTRPSLRIPPLQDPFPPLSIISRRPSPLEPNARVNIDNEANKLTGRQRALAQAKPLGAPEHNELEASITAKPAALPEAQETEHPKERPRKKQKLYDHEQIADFVQLPRPQTKLREDKPRPFQPISVLNELHEPPPSAALFPPITPNAVQEEHDKYHSGAGQFPNQTKDPGRPPARKRKPRVRSTGSCTAKRIYTRERTAWTEEETDQLVKGVAIYGMGRWKSILNHPELHFREGRTNMDLKDRFRVVFPPNARHKWTRAMPEPPDDDQEAPSGYKFATKGTAKKSPLRAPKRLWTEQEDTELEKGFQLYGFQWNLMLKDPALNFDQRSCGQIRDRFRLRFPGLYKQQDSTASPKENQTSPKKKKHAKSSTRTRKTVDDPNEPANEESDGGARDKTGSKTVPASHTEEEDHRLSNSILHNDWDWDENLTLAPLAWEDMATRPMFPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH91</accession>
    <name>A0A8H6CH91_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450 monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000215</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152590.1">
        <property type="nucleotide sequence ID" value="XM_037291155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH91"/>
    <dbReference type="GeneID" id="59328634"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11058">
        <property type="entry name" value="CYP60B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF210">
        <property type="entry name" value="CYTOCHROME P450 MONOOXYGENASE ASQL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="56973" checksum="33D8390A513F0776" modified="2022-01-19" version="1">MSSPLAISASQFMSTTGAILVLYYVVSAIYNIYFHPLSKYPGPKMTAISRLPLIYSTITGNTWRFTGKLHAQYGTIIRIAPDEIATTSSGAWKDIYTSKPLLPKDPFSQTPPMNGAESLFTAAGETHQRIRKTFINAFSDRALKDQTPIIDSYANLLMTRLHREIGKSPEGKVDMAKFYGYVTLDIIADLTFGESFYGLEGDNEHSWILGFFLGAKFGSVRNSLSRFHPIDKIFGWIFLRLTAKNRARSWQVATDKISRRLDMGDLGPSRSDFITPVVGRLDETQQKGITRKELNTNALAVVIAGCQLTTVALATSTYLLMRYPETLKQLTKEVRESFESEAGITVPSTMNLPYLTAVINETLRIHHPTPINLPRVIPPEGQTIDGHWIPGGNIIGVNLQNIQNDPNNWVEPRVFHPERFLPETDSRYQKRFDKDDKQAFQPFSVGGRNCMGGKVFLAEARMILSKMVWNFDLEMADPSDGNWMNQKAYLVFEPKALMVKLKAKALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHB4</accession>
    <name>A0A8H6CHB4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED6</name>
        <name evidence="10" type="ORF">HO133_000066</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHB4"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.580">
        <property type="entry name" value="Mediator complex, subunit Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007018">
        <property type="entry name" value="Mediator_Med6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038566">
        <property type="entry name" value="Mediator_Med6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13104">
        <property type="entry name" value="MED-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04934">
        <property type="entry name" value="Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="173"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="255"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="262"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6223224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="362" mass="38965" checksum="29617F8D62078ABE" modified="2022-01-19" version="1">MAQEPPLDEIQWRSPKAQEMGGIHTNTVLPYFAESPFFDATSNNAILTTQALYNPNMFYIIQTREAFEGRLRTMQGLEFMVTHDPSENDSKQDHSGVWVIRKQNRRKRQGAPDEVTGISSYYVVGENVYLAPSVGNILGSRLLSTVASLTKLLSTASSLPSFTPSLGHTYLPPVSKIPTPGSSFQATQASKEGTPMPAGGTPLPATQETTTSAKTSKVTSDMGLQGVHMLAYSYDLSDRYANEYMDENPIVGEPGSFKLSKSHNTSLTTSMNTSKTSSQAQEVSKPSSQSLEVATPAKSAAPTPQLKTEDLPAPLRKSTKSSEKSPTTPGTKEKKSRRKSKAAGDKGATTPKATTPKPTTPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHI8</accession>
    <name>A0A8H6CHI8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152897.1">
        <property type="nucleotide sequence ID" value="XM_037291462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHI8"/>
    <dbReference type="GeneID" id="59328942"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="230" mass="25287" checksum="6BFF9D04117C7FF1" modified="2022-01-19" version="1">MVSAGHICLRPRELKPMPAPVCTFLRTTDQGFNTGAELETLEDNAGVTQRRIELRGRDAIGAGRGGGGKAKRVKLQTVPLVQPITRVRFPKEHPTAQTDVPRHQPVRVRFPKGQSTYLATNQFASGFRKGNRRTSPPTSPHTEDSLSCEASEREHYHTKRTTNLASPKASKRADAVAIFGEESEDEAVVDANAVVTAEDSDYAEVHMPSRKAPFHRVETKETGVHSTPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI25</accession>
    <name>A0A8H6CI25_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000419</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. TCR/Tet family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152793.1">
        <property type="nucleotide sequence ID" value="XM_037291358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI25"/>
    <dbReference type="GeneID" id="59328838"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF102">
        <property type="entry name" value="DRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G08530)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="141" mass="15050" checksum="816DF3ECA47674A0" modified="2022-01-19" version="1">MAIPVFVVDAFTGWVISAMGMYTWFIRGGFHIILGAGIDPNFQALTIAFQSVVKSSDVGTATSTFSFVRNLATSTGLILGEVVFQNVMRGQYNKLSGLSGPRLTGTLANGGAASSVFTVNKLPADQITAARDAYYMEIRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIF8</accession>
    <name>A0A8H6CIF8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153144.1">
        <property type="nucleotide sequence ID" value="XM_037301510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIF8"/>
    <dbReference type="GeneID" id="59339048"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF19">
        <property type="entry name" value="AARF DOMAIN-CONTAINING PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="1">
        <location>
            <begin position="98"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="415" mass="47475" checksum="B45733BEA3537571" modified="2022-01-19" version="1">MDLLCAESLSPQTKFIRERRIERLHVGKTYVNYQCTLRSMAQDGHEWQAVLKACHKRCAERTLEVLEQNGSIFIKLGQHLSSMNYLLPEEWTKTFVPLQDHCPVSSYDSIAAMVLKDTGSPLDEHFDHFAKTPIGAASLAQVHLATMKATGQTVAVKLQHPALEEWVPLDLFLTRTTFSIVKFFFPEYDLEWLSNEMESSLPLELDFALEGNNALHTKEYFYKMHDSPLVVPDVIWGMKRILVMENVSGHRTDDLRYLDSNGIDRDEVSAALARIFNEMIFGDTPLHCDPHGGNIAIRKNNDRRKPNFDIILYDHGLYRDIPKQLRRDYAKLWLAVIDADEIGMRKYAYDVAGITDDQFPLFASAITGRDYINATNSVVSKRLDQEKEEISGAMGEGMLQEVIPWWTSVDEEYHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIK9</accession>
    <name>A0A8H6CIK9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010686</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153172.1">
        <property type="nucleotide sequence ID" value="XM_037301538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIK9"/>
    <dbReference type="GeneID" id="59339076"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12306">
        <property type="entry name" value="RRM_II_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="67"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="184" mass="20524" checksum="864EDC526D93F6D9" modified="2022-01-19" version="1">MSDNGVAAEDERSQIKDEGGDDEEEISAMKRRVAEMEAEAAKLREMQASLDQQDNDLREDKEDIDARSIFVGNVDYGASPEEIQAHFQSCGSINRVTILLDKFTGHPKGYAYVEFTEPNLVAQALVLNESMFRGRNLKDLLVVEVDSGEGVMIEEEDMIVDMPLEADIVEDIEVDLGATLHTER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIN0</accession>
    <name>A0A8H6CIN0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Haloacid dehalogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily. S-2-haloalkanoic acid dehalogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152976.1">
        <property type="nucleotide sequence ID" value="XM_037301342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIN0"/>
    <dbReference type="GeneID" id="59338880"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019120">
        <property type="term" value="F:hydrolase activity, acting on acid halide bonds, in C-halide compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006328">
        <property type="entry name" value="2-HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051540">
        <property type="entry name" value="S-2-haloacid_dehalogenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01493">
        <property type="entry name" value="HAD-SF-IA-v2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01428">
        <property type="entry name" value="HAD_type_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316:SF3">
        <property type="entry name" value="HALOACID DEHALOGENASE, TYPE II (AFU_ORTHOLOGUE AFUA_2G07750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316">
        <property type="entry name" value="HYDROLASE, HALOACID DELAHOGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27913" checksum="117B79B5744A93A9" modified="2022-01-19" version="1">MSPVSTSPAVPKAIVFDLMGTCVDWYSSILPALESAPSIPALPSESLPQLAADWRAGFFKETRTRRDASQLVEDIDVTHRRVLNWLLLARGVTPAQWDDGVRKKLVGAWHEQRGWPDVASALERLKQKYFIVVLANGSTRLQLDLMKASNLPFHMLFSSQLLGKTKPDAEIYTKALELMQVAPSEAIMVAAHAYDLRAAKTVGMKTIYVQRTTEDFTENIDQVRDEVDFFIDGTSGDEKCGFGELADILQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJR5</accession>
    <name>A0A8H6CJR5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010012</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153685.1">
        <property type="nucleotide sequence ID" value="XM_037300868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJR5"/>
    <dbReference type="GeneID" id="59338404"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08831">
        <property type="entry name" value="ArfGap_ArfGap2_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000013">
        <property type="entry name" value="Putative Arf GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="11"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="281"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="490" mass="52696" checksum="831FF07BF2CE7510" modified="2022-01-19" version="1">MSSSATKAQSQKIFEKLKTKPANKVCFDCGTKNPTWSSVPFGIYLCLDCSSNHRNLGVHISFVRSTNLDVWQWDQLRIMKVGGNESATKYFQSHGGTAALANKDPKTKYTSNAATKYKDELKKRATADAREYPEEVVVTDIVAATPTDGTSIPSEPGDDFFSSWDKPSIKRPSNPPSRTQTPPVISRTASPFLNPGANSNGAPRSKSPLPGSESESSLAPSRAVPSAVIRKTTATGNARKTNVLGAKKTQKLGAKKLGGAEAVDFEAAEKKAKEEAERIEKLGYDPEAEEAAVQKTVRSASISEKTKITAPTPVNPVKGYGVPKHERTKSEMERLGMGMGRLGFGQVGGSKPAPAAPKKMGFGSVNASRAVEEANDEKYARQKFGTQKGISSDEFFGRNNFDPSTQTEAKQRLQGFEGASSISSNAYFGRPEDDIPQADEYGNYGDLEGAARDFVRKFGVTAGDDLENLTNVLGEGATKLQGAIRNYLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK23</accession>
    <name>A0A8H6CK23_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Signal recognition particle subunit SRP72</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010178</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SRP72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153850.1">
        <property type="nucleotide sequence ID" value="XM_037301033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK23"/>
    <dbReference type="GeneID" id="59338570"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000512">
        <property type="entry name" value="Signal recognition particle subunit SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013699">
        <property type="entry name" value="Signal_recog_part_SRP72_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026270">
        <property type="entry name" value="SRP72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031545">
        <property type="entry name" value="SRP72_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094:SF9">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08492">
        <property type="entry name" value="SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17004">
        <property type="entry name" value="SRP_TPR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038922">
        <property type="entry name" value="SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="Signal recognition particle SRP72 subunit RNA-binding" evidence="11">
        <location>
            <begin position="545"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="537"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="559"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="569"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="593"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="649" mass="70330" checksum="563205FBF8DD0226" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK32</accession>
    <name>A0A8H6CK32_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vacuolar calcium ion transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010209</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Has a role in promoting intracellular calcium ion sequestration via the exchange of calcium ions for hydrogen ions across the vacuolar membrane. Involved also in manganese ion homeostasis via its uptake into the vacuole.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153881.1">
        <property type="nucleotide sequence ID" value="XM_037301064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK32"/>
    <dbReference type="GeneID" id="59338601"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000026">
        <property type="entry name" value="Vacuolar calcium ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004798">
        <property type="entry name" value="CAX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00378">
        <property type="entry name" value="cax"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF14">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 10" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="107"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="307"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6225014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="473" mass="52053" checksum="3D983E58D3F3B570" modified="2022-01-19" version="1">MLSRFRGKPEPNLQAGVREEAVGLPESAHVQHEKPQHHMHMPGFNHNGHKITKGIHPDGESGRGGVHPWHFLRICFRSSSHLSLVVNILWPFVPAAIAIHFARPDLHLWIFILNYVAMVPTANLVGFAGQELARKLPKVLGLILETFLGGIVEIVLFMVLLHNSVNNSLIPVIRSAILGSILANLLLCLGFCFFAGGLRRNEQEFDEVISEVGNGQLLMAGFGLLIPSAFYSSLRGSIEDSVLDANVLHISRTTSVILLIAFLIYVWFQMHTHHGLYADSLEKDEERDADRHIDLAKDKLTLTECIVATLIALTCVSLHAVFLVEEIEHVVHEHGVSDAFMGLILVPLVEKAAEHLTAVDEAWDNQMNFALAHCMGATIQTALFNSSLVVIVGWGLNKAMDLNFEIFNIVVLIMAIIVVGNFLRDQKSNYLEGALCILIYLIIAIASWYYPNPTPGTTNTADGSGIASNGTIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK60</accession>
    <name>A0A8H6CK60_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010142</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153815.1">
        <property type="nucleotide sequence ID" value="XM_037300998.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338534"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039295">
        <property type="entry name" value="MSB2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778:SF1">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034157244" evidence="3">
        <location>
            <begin position="22"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1240"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="969"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1306"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="689"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="970"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="984"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1003"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1049"/>
            <end position="1060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1344" mass="129662" checksum="193FB35C6161641C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK65</accession>
    <name>A0A8H6CK65_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010039</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153712.1">
        <property type="nucleotide sequence ID" value="XM_037300895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK65"/>
    <dbReference type="GeneID" id="59338431"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF17">
        <property type="entry name" value="PROTEIN SIMILAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="229"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="469"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="425"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="525"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="621" mass="68438" checksum="3A8C49D8FEC0D29C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK79</accession>
    <name>A0A8H6CK79_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit beta'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_009993</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasmic vesicle</location>
            <location evidence="1 13">COPI-coated vesicle membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="13">The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153666.1">
        <property type="nucleotide sequence ID" value="XM_037300849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK79"/>
    <dbReference type="GeneID" id="59338385"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22947">
        <property type="entry name" value="Coatomer_WDAD_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000016">
        <property type="entry name" value="Coatomer alpha subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000001">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016453">
        <property type="entry name" value="COPB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF2">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005567">
        <property type="entry name" value="Coatomer_beta'_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="5 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="180"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="16">
        <location>
            <begin position="318"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="17">
        <location>
            <begin position="594"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="817"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6224799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="854" mass="95114" checksum="74F229C116E61510" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKH3</accession>
    <name>A0A8H6CKH3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154152.1">
        <property type="nucleotide sequence ID" value="XM_037301335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKH3"/>
    <dbReference type="GeneID" id="59338873"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="167" mass="18909" checksum="E3159F7B38F6B05A" modified="2022-01-19" version="1">MADQDPKITLLRSKLALLMGREECPVYDSIKAQHPHALEEPWSEDMSGPHKRVVFNNKTFAMHQVLTQEEAYKRTFQPHAINCGENIKLHLDDNLFRDLEQAETMDLSTLKRSEAITRRAVKSKWFYALTGWVNEINGGPGKMGPSPSDNITIEGKEDASMSSRKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKX6</accession>
    <name>A0A8H6CKX6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009449</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154158.1">
        <property type="nucleotide sequence ID" value="XM_037300310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKX6"/>
    <dbReference type="GeneID" id="59337844"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000085">
        <property type="entry name" value="MFS peptide transporter Ptr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="533"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="563"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="616" mass="68152" checksum="E9DF7AE620EE85C4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL10</accession>
    <name>A0A8H6CL10_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009497</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ATG33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154206.1">
        <property type="nucleotide sequence ID" value="XM_037300358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL10"/>
    <dbReference type="GeneID" id="59337892"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051668">
        <property type="entry name" value="ATG33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37278">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37278:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="179" mass="19244" checksum="7F47900D91AB74E6" modified="2022-01-19" version="1">MSRTTATICKFIGTISLGLLTGVSYTTTTTTLPPIQSLPSAQPASTTFQHLRALAHRHQLLLSTLSSTSLLLAYALSPPRSRHPYLLWATLAIGLGYTKEIYAGVVGKVKGKGDESRARSRTPDSSDEEWEVEGASTGSLNGEAVREGMEKWRGKMVGRTVVWGVGWLVTVVGLWGDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL46</accession>
    <name>A0A8H6CL46_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PLC-like phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154101.1">
        <property type="nucleotide sequence ID" value="XM_037301284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL46"/>
    <dbReference type="GeneID" id="59338822"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF80">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26146">
        <property type="entry name" value="PI-PLC_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034303307" description="PLC-like phosphodiesterase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="38236" checksum="654A3AEDF7FC6B32" modified="2022-01-19" version="1">MQSILCWSLIIQAALGAILTARNATIACNNSPQLCSRAYSNITQLGAHDSPFLNNASASNSITFSDAGNQNVNSVAQLAAGVRLLTAQVHNNNGAWHLCHTSCSLLDAGTLSAWLSTIKTWMDDNPNDVVTILLVNSDDASAEDLNAEFEAANITSYGYQPPSSTTALATWPTLQEMISNNSRLVTFVADLDPSTNTVAPYLLDEFTFVFENPYNVTSSSNFSCVADRPPAVFEQTATAISSGRLPLVNHFLDTNEGLGIDIPDTANVTTTNGKTGAGSLGAAASDCSAAYGKAPAFLLVDFFDEGFAISVVNGLNGISTTAVGQTQPSVTPTSSSGASDISNLGFGSYGWLVVLFVTWLAHQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLA6</accession>
    <name>A0A8H6CLA6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154169.1">
        <property type="nucleotide sequence ID" value="XM_037300321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLA6"/>
    <dbReference type="GeneID" id="59337855"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="340" mass="37649" checksum="3E471F1D10D6DE02" modified="2022-01-19" version="1">MELNVQGPRPVNTAPTTTLDIDGESASSTKSTNREAHAINGSLKPHPPKPSLNPQTPASTTPTPLGTLSVLPRELRDEIYSHVCEREYDFGRFSDRLHRKWHSDGNTDLPMLTLSKTIREELLAVLHAEAVFSIRSARYFQNKAWTRNDIPFIDHIQRVGLSTAVCALSDEHCNESMLDPREANEQLSKMRAEPTSFFAGTEVPRKTCVIEFILCTPKTMLIRQSPLFDAIKHLTGFKTVTLKISSIREHWGPQDALIYIGEDPSFMDHAVGFAAIIDAIGSALEPSLGPNVISEAIVLQGIGWKITFHPRDYLSKQEKPEAGSGAQLEEAADTLTALEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM09</accession>
    <name>A0A8H6CM09_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154602.1">
        <property type="nucleotide sequence ID" value="XM_037300754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM09"/>
    <dbReference type="GeneID" id="59338290"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="104" mass="11252" checksum="6FE3C433C0B0AC10" modified="2022-01-19" version="1">MSQNFKTIDLQPQPEKPMGAHLRDGDSKDIISEQPSSRKMSMQAEPQVSMRGGGVIGDWYVARHSQLSISDVGERAASSPTPSPASHAAELRFQSSIAPHLRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CME8</accession>
    <name>A0A8H6CME8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="5">HMG-CoA synthase</shortName>
            <ecNumber evidence="5">2.3.3.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl coenzyme A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009813</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154520.1">
        <property type="nucleotide sequence ID" value="XM_037300672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CME8"/>
    <dbReference type="GeneID" id="59338208"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000590">
        <property type="entry name" value="HMG_CoA_synt_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01226">
        <property type="entry name" value="HMG_COA_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="7">
        <location>
            <begin position="179"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="459" mass="50978" checksum="A0FAAA6BA938F439" modified="2022-01-19" version="1">MASRPQNIGIKAIEIYFPSQCVDQAELEKFDGVSQGKYTIGLGQTKMSFCDDREDIYSFALTTVSTLLRKYSIDPKSIGRLEVGTETLLDKSKSVKSVLMQLFQDVGNTNIEGVDTVNACYGGTNALFNAINWVESSAWDGRDAIVCAGDIALYKKGNARPTGGAGCVAMLVGPDAPLAFEPGKRGTYMQHAYDFYKPDLTSEYPVVDGHFSVSCYTEAVDACYKAYNEREASLQKSGIANGVHNTPEDVTPLDRFDHVLFHSPTCKLVAKSYARLLYNDYRVNPENPTFESVPKELRDLSYEESLTDKTVEKTFMALSKKKFQTRVQPGVQVPTMCGNMYCASVYGSLVALLSNAPREGFTGRRVGIFSYGSGLASSFFSLKVVGDISPMVKQLNLHERLDARRTVPPETYDEMCLLREKAHLQKGYTPKGNPETITKGTYYLTEVDEMFKRKYEIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMG9</accession>
    <name>A0A8H6CMG9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">O-methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154736.1">
        <property type="nucleotide sequence ID" value="XM_037299910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMG9"/>
    <dbReference type="GeneID" id="59337444"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF17">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="5">
        <location>
            <begin position="67"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="182"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="44522" checksum="1EED08A7D3B46855" modified="2022-01-19" version="1">MFSPAAEQVDRASLLIKDLSSLQTSLLETGDSTRQEMIAKARALISTLEAPMESILWMGWAEPARTATIRISIDIGLFEKLAADDGKAKNSQQLADATKTDSALIGRMLKHLASMFIIRETLQDTYAPTPLSNALTDQIFYDGVIFTFDAQLPSHARLPAYLRSTNHIIPTDGSVFKYSHKSELGIFEWIAADPRMQTAFQNHMAGYSMGRPHWMDDGFYPVTERLRGVGEGEVLIVDVGGATGHDLAEFHDKAPTISGRLILQDQAQAISSIREGDRPGIECTVHDFFTPQPVRGAKAYYMHSVLHDWSDDNARKILTQLKEALKPGHSKILINENVVSDQDADWKITSLDWTMMVCLGSCERTETQWRDLVVSVGLKISGIWKGEGATESLIEIVLADDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMK3</accession>
    <name>A0A8H6CMK3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154963.1">
        <property type="nucleotide sequence ID" value="XM_037300137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMK3"/>
    <dbReference type="GeneID" id="59337671"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08241">
        <property type="entry name" value="QOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF4">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="12"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="336" mass="36067" checksum="E2B3D735E30F46C7" modified="2022-01-19" version="1">MRAIQVKEYLKGPQDLTVSSLPNPTPSADQYLIAIHATATNFFDLLQIRGKYQHQPPFPWISGSEFSGVVLSVPSSNPSPAFKKGDRVFGAAQGGYSTHVCAAETALRPVPKGWGFVDAAGLMVTAPTSYGALVTRAGVKKGDYVLIHAAAGGVGLAAVQVAKAFGATVIATAGSARKLEVAKGFGADYAIDYNTKGWEEEVKKLTPRGRGVDIVYDPVGMVNTSLKCTAWNGRILVIGFAAGDIEKVAMNRVLLKNVSLVGLHWGMYTREEPETVEEVWRGLFRLMDEGKFRGTVYSDRIFSGLESVGEALGMLGRRETWGKVVVSVPQDGQSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN63</accession>
    <name>A0A8H6CN63_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154867.1">
        <property type="nucleotide sequence ID" value="XM_037300041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN63"/>
    <dbReference type="GeneID" id="59337575"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="184" mass="20320" checksum="658F0E7B42A136C6" modified="2022-01-19" version="1">MADKQRSQAQTTTKNTTHYNPEHEDQGNEEQASYTTTQQDPGSLSSAQPNSTPAMADKQHSQAQTTTENTLHHNPEHEDKGSKEQASYTTTTQQDPDSVNSTQPNSTTRKLSAAQKKSRGSQLLAPVAEKAQRSRNLTTHKVIFDEMAVTEEGDLIDIGLTQSTQPAQAVRKMYRAALGRPWEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNM5</accession>
    <name>A0A8H6CNM5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008436</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155303.1">
        <property type="nucleotide sequence ID" value="XM_037299302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNM5"/>
    <dbReference type="GeneID" id="59336832"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="116" mass="13628" checksum="AB41FB1082FC9E2A" modified="2022-01-19" version="1">MAPFSDPQMHQPMDQYWKHDHDPQFLYRGSQEDIVKELEAWVVHRKEVYKERLRSRGLPPRYPQVPTHHLAGTPVTARGRRRRLLFPGHLGRSDATLAVLEAREKEERLRANAAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP22</accession>
    <name>A0A8H6CP22_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008449</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155316.1">
        <property type="nucleotide sequence ID" value="XM_037299315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP22"/>
    <dbReference type="GeneID" id="59336845"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="441" mass="47941" checksum="AFAA4DD2E5A6EDD1" modified="2022-01-19" version="1">MPVAPLAARATNTSKPTGLQSGSTNPVVIGIAIAVALTFILIAVYVYVAKRRPPNYSTSPKKDPRWRSWTNKAGRLLPFKKKSGARSIQDQETSYVATNTSTRESTMHSLAEIPPSDGVNRNTSIRSIMTLPPYHPTPHPHERLIAREGERGGVDTVVEFPETAEEEEERREQDMESLYQIRQTRRQEINERNDRRRERQEAREAGDWARLEQLRLQSEARARARAGSSASAGSSTTSLPIAAASASSMIADHQARASSNDRRVSSVSYAELGLARHDGSRLRADSVESDHRPLLDSAASMGGDQRNASSSRRSSLFHLPTHRFHSHARAASAESVMTTDSELADMTPQSSSEDRSGSGSDPTMRTPSVGTSSEPSPPMGEPPEYEDAPPYTSPVAGRGEGVPRLPSLRVGERLPAIQITGSTPAATPVVGTPAEHSDQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP40</accession>
    <name>A0A8H6CP40_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7580 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155148.1">
        <property type="nucleotide sequence ID" value="XM_037299147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP40"/>
    <dbReference type="GeneID" id="59336677"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056002">
        <property type="entry name" value="DUF7580"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186:SF4">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24476">
        <property type="entry name" value="DUF7580"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7580" evidence="2">
        <location>
            <begin position="52"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="312" mass="34111" checksum="13CC8487B56CB3CB" modified="2022-01-19" version="1">MSLVVPRNSIPQPPVIRTSFKSSMRIPSLSPLPSRPKVSWTNPTPPAADSTQQTTGPTVITDLCSTVATCDPASNIFGLLEGDESSYILQRGAKTKPPGQAYQTVSPVSLESLSNRSSGFLLDRRQRYRIAYTLASSHLQLYPSPWLHSHWSKKDIVFNIDPQDAHSVQLDQPYMLHAVSTQAAPSPSSSYASSDRSLPTLGILLIELCFGTALEDHAMRQEFHTSGGQQTANPDLAAALDLAVALEWSQSVCGEAGETYADAVNWCLRGRFAGAKDDGWREELFANVVRPLRFCHEQLHAMGGEDWLKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP64</accession>
    <name>A0A8H6CP64_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Nucleolar GTP-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008446</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Nucleus</location>
            <location evidence="2 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class YlqF/YawG GTPase family. NOG2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155313.1">
        <property type="nucleotide sequence ID" value="XM_037299312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP64"/>
    <dbReference type="GeneID" id="59336842"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01858">
        <property type="entry name" value="NGP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000559">
        <property type="entry name" value="Nuclear/nucleolar GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024929">
        <property type="entry name" value="GNL2_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012971">
        <property type="entry name" value="NOG2_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF9">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08153">
        <property type="entry name" value="NGP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="9">
        <location>
            <begin position="224"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="508"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="518"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6227005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="582" mass="64103" checksum="73EC16FB3F140854" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQD3</accession>
    <name>A0A8H6CQD3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008769</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the EMC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155634.1">
        <property type="nucleotide sequence ID" value="XM_037299633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQD3"/>
    <dbReference type="GeneID" id="59337164"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055217">
        <property type="entry name" value="TPR_EMC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22890">
        <property type="entry name" value="TPR_EMC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="EMC2 TPR-like" evidence="5">
        <location>
            <begin position="111"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6227326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="321" mass="35786" checksum="3FD9DDD36EB740B5" modified="2022-01-19" version="1">MSLSSLLRQSNAEALSLSQQTAQYLRKHASTGISTINIPFLSYPESTDVWLMYEKLLLSCLRTGDDKAAFNCLEKLIDRFGATNERVMGLRGLYQEAVAKDDAALERVLQEYDEVLAEDTVNTPVAKRRIALLRSLSRTTEAIDALTELLESSPTDIEAWTELSDLYLSQNLFPQAVFCLEEVLLVAPNAWNIHAQLGEVLYISAMNGNESSEGRSLAEAARRFCRSVELCDDYLRGYYGLKLTSDRMLSTNPNDTRSTGQVATVDNGELPMLPTAVVRRLNEQATFKLAEITRTSNSSCNVANINAAKDLLNRSTQRKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQQ8</accession>
    <name>A0A8H6CQQ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nitrate/nitrite transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_007848</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156054.1">
        <property type="nucleotide sequence ID" value="XM_037298718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQQ8"/>
    <dbReference type="GeneID" id="59336245"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015113">
        <property type="term" value="F:nitrite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000382">
        <property type="entry name" value="Nitrate transporter CrnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004737">
        <property type="entry name" value="NO3_transporter_NarK/NarU-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00886">
        <property type="entry name" value="2A0108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="HIGH-AFFINITY NITRATE TRANSPORTER 2.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="487"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="35"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="254"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6228120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="515" mass="55446" checksum="297EA60FFC2C470D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR28</accession>
    <name>A0A8H6CR28_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007736</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155942.1">
        <property type="nucleotide sequence ID" value="XM_037298606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR28"/>
    <dbReference type="GeneID" id="59336133"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034898000" evidence="4">
        <location>
            <begin position="25"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="353"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="386"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="421"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="457"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="490"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="571" mass="62866" checksum="5FFEE48A02495483" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRF8</accession>
    <name>A0A8H6CRF8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008044</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156248.1">
        <property type="nucleotide sequence ID" value="XM_037298912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRF8"/>
    <dbReference type="GeneID" id="59336441"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03761">
        <property type="entry name" value="proteasome_beta_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000013">
        <property type="entry name" value="Proteasome subunit beta type-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF3">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6228314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="282" mass="30846" checksum="7F42F2B44AEB53DB" modified="2022-01-19" version="1">MDALVATYSRPAFEDEGYSSDEHQELIQAKPSLSLNFALPPMRNPSAFLRALTDDHANPSCPIKLAHGTTTLAFRFQGGIIVATDSRASAGDWIASQTVKKVIEINNCLLGTMAGGAADCQYWLGYLGMQCRLHELRHKRRISVAAASKILSNLVYSYKGMGLSMGTMCAGVTPQEGPALYYIDSEGTRLAGNLFCVGSGQTFAYGVLDAEYHYNLSEEEALELGSRSILAATHRDAFSGGFINLYHVKEDGWVKHGFRDTNPIFWKTKLEKGEFSNVTANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRL7</accession>
    <name>A0A8H6CRL7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Integral membrane bound transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008134</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156338.1">
        <property type="nucleotide sequence ID" value="XM_037299002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRL7"/>
    <dbReference type="GeneID" id="59336531"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023244">
        <property type="entry name" value="Brefeldin_A-sensitivity_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052430">
        <property type="entry name" value="IVT-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049453">
        <property type="entry name" value="Memb_transporter_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804:SF1">
        <property type="entry name" value="DUF2421 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13515">
        <property type="entry name" value="FUSC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02047">
        <property type="entry name" value="BREFELDNASP4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="660"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="691"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="737"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="Integral membrane bound transporter" evidence="7">
        <location>
            <begin position="683"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1043" mass="115553" checksum="A6BB9C38CFED6204" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRU9</accession>
    <name>A0A8H6CRU9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156140.1">
        <property type="nucleotide sequence ID" value="XM_037298804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRU9"/>
    <dbReference type="GeneID" id="59336333"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="649" mass="71147" checksum="A37A00BE53CEE216" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSB8</accession>
    <name>A0A8H6CSB8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156380.1">
        <property type="nucleotide sequence ID" value="XM_037299044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSB8"/>
    <dbReference type="GeneID" id="59336573"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="104" mass="11613" checksum="DD83B15A8C7762FE" modified="2022-01-19" version="1">MDSWLQVVEKDKTRIMHMRLVDHQGQFKYLLVTHYNEKILEPDVASDSSSAIRRDKGNDATEPPTSFISDVGASDSDTTISSTPSSSAESRSRNLAKAKARVDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CST5</accession>
    <name>A0A8H6CST5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006983</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156514.1">
        <property type="nucleotide sequence ID" value="XM_037297879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CST5"/>
    <dbReference type="GeneID" id="59335383"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF8">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="8840" checksum="9C4C69299BFF6C15" modified="2022-01-19" version="1">MKPTKALLARSVWKGPNIVPLPIIKSKPGERTPPIKTQARAATILPNFVGLKFQVHNGKIYHDVTITEEMVGHKLGEFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CU17</accession>
    <name>A0A8H6CU17_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006998</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156528.1">
        <property type="nucleotide sequence ID" value="XM_037297893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CU17"/>
    <dbReference type="GeneID" id="59335398"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39336">
        <property type="entry name" value="PYRIDOXAMINE PHOSPHATE OXIDASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G11440)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39336:SF1">
        <property type="entry name" value="PYRIDOXAMINE PHOSPHATE OXIDASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G11440)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="2">
        <location>
            <begin position="9"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="252" mass="27913" checksum="42968A202280B8CB" modified="2022-01-19" version="1">MVQFWDSIPDNLRDWALDQQVFFTASAPLTGKYVNISPKGLPSSTFSIFDPNHAAYVDATGSGSESISHVYENGRITIMFCSFAKSPRILRFFCTGKVVEWDDPKFEALLQRMGKERIEGARAVISLDVFKVQTSCGFGVPLLTTAPTSEGTTSGETEPILLDRDTMGHWSHRKVETNTLLDYQAEYNSSSLDGCPGMRSAMRDRGDRVWVTLLKARIRRIGTQMEAVLVGIVLGVALLAFAQVTLRSLGFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7W2</accession>
    <name>A0A8H6F7W2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prion-inhibition and propagation HeLo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147962.1">
        <property type="nucleotide sequence ID" value="XM_037296781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7W2"/>
    <dbReference type="GeneID" id="59334281"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1020">
        <property type="entry name" value="Prion-inhibition and propagation, HeLo domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056002">
        <property type="entry name" value="DUF7580"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029498">
        <property type="entry name" value="HeLo_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038305">
        <property type="entry name" value="HeLo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542">
        <property type="entry name" value="HELO DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542:SF1">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24476">
        <property type="entry name" value="DUF7580"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14479">
        <property type="entry name" value="HeLo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prion-inhibition and propagation HeLo" evidence="1">
        <location>
            <begin position="6"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7580" evidence="2">
        <location>
            <begin position="401"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="622" mass="69718" checksum="B078D50EE3D05001" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F802</accession>
    <name>A0A8H6F802_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148020.1">
        <property type="nucleotide sequence ID" value="XM_037296839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F802"/>
    <dbReference type="GeneID" id="59334341"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="256" mass="28553" checksum="3039C0BC26611A81" modified="2022-01-19" version="1">MTTQNRAALMLSSLKKTIIEVSGSYSNRGSGYYDAPPVEDKTPLVQDLEEMIVSPLTTKDEKVVLIDTLMVVRVLQRLKEEEWSDRLKEPATSNMDGKESLTQKSRRSEAAHVAIAMLKAAIIQACYENHWRDGAFWSIRHSSSDVDNDLERLEGSMKTTEAEKAIFKDTRSKVADMRAYLEGRPTNLSSVDDLDWVKRLKSVFESQVGEVLEAGEVSTVQKNGPNRKPALSRKGDEDDGFESQKVYGGSGGSPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F846</accession>
    <name>A0A8H6F846_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006138</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147614.1">
        <property type="nucleotide sequence ID" value="XM_037297040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F846"/>
    <dbReference type="GeneID" id="59334542"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF120">
        <property type="entry name" value="HIGH-AFFINITY COPPER TRANSPORTER CTRA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="172" mass="18828" checksum="0F554BB0ED04774E" modified="2022-01-19" version="1">MSMSTMQMVFYTSTTTPLYSTSWTPSTTGQYAGTCIFLIVLATFFRALFALRAVQERRWQQMESRRRYIIAGADEKENDSRSSADGSKMSVSSEQSNEGSMAVLKGNVSAVRPWRTTQDVPRACLDVVIAGVGYLLMVAVMSLNVGYYMSVLGGTFLGNLLAGRFSAAPTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F872</accession>
    <name>A0A8H6F872_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable aspartic-type endopeptidase OPSB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Probable aspartic-type endopeptidase opsB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005675</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147763.1">
        <property type="nucleotide sequence ID" value="XM_037296582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F872"/>
    <dbReference type="GeneID" id="59334080"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000011">
        <property type="entry name" value="Aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13668">
        <property type="entry name" value="Ferritin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034462612" description="Probable aspartic-type endopeptidase OPSB" evidence="9">
        <location>
            <begin position="28"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="10">
        <location>
            <begin position="76"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="786" mass="82397" checksum="97E8CFF14F8A32E1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8W7</accession>
    <name>A0A8H6F8W7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149072.1">
        <property type="nucleotide sequence ID" value="XM_037295025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8W7"/>
    <dbReference type="GeneID" id="59332515"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053175">
        <property type="entry name" value="DHMBA_Reg_Transcription_Factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38791:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR DBAG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38791">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="472" mass="52532" checksum="2163DEC8FFFB4F51" modified="2022-01-19" version="1">MTVAAAARYRAEQDAVFRNETSKVVAKAQTGTKWEISMPVSPIRPIELTGDGDVRNPAIDFSAATVQMVERTKVTIRTLSVPLEDEATYYFFHNFVPADPASLTTYSDVLPTVYRQDSSFNALPKIIEAIGLAGISNVKQAPELMVAAGQKYARVLRAITASIQDSKETVTCSNPESMRSWTNHVNGATALVRLRGTDQLRTKVGRKLFDNLVVQILIDCLQRRQMIPPDVIEWAEIAAEGETKKAVPERQLIQIIGRLCALRAAVDRPETNDIGVVTLANFIDSDLEDWKNSLPPAFSYSIKQSPNTENVFSNTYHVYKGTWALSVWNVYRCARILTQQVITTWLSRNSMPNPALDQSQRRQSKVLLANLAYDICASAPFILGASNSSVYSSRLPRAAAGASLLWPFYLVATMDQCLTGIRAWIITRLALIGRLMGIKQAESLANVLRTKREITAWDKFETARADEVLDEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9V2</accession>
    <name>A0A8H6F9V2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PIG-P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003863</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149473.1">
        <property type="nucleotide sequence ID" value="XM_037294785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9V2"/>
    <dbReference type="GeneID" id="59332274"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="7">
        <location>
            <begin position="180"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="117"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="322" mass="34755" checksum="4353CC98E4F12614" modified="2022-01-19" version="1">MAPQSRGRAVSLSLQSFDLAQPVTPAEETPRALGMDHAFASSSSSAYGDEEKDEEDEEEEEDSGTQSQYTLDDASSDESSGAESGNGAGGPGHPPFLHHSNSSFLMSNAFAPPYYNRPPTPLPASPSLTSLLRPPFSTTTSRPTTPDSSENDTPNDTEAAVAKSARIATTVPRASPKVPTYEYYGFVLYLLSSLAFLMYLLWSYLPSPFLHQLGIYYYPNRWWSLAIPSFLVMTLVYIYVALASYNTGYLTLPMSSIENIVDDAAHVAVLDSHGLIQRKAGQAGAAIGINDAKEVDWGTLWNEGTDAVMDVPVGGVCEILYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA09</accession>
    <name>A0A8H6FA09_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150257.1">
        <property type="nucleotide sequence ID" value="XM_037293428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA09"/>
    <dbReference type="GeneID" id="59330915"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000026">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="6"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="139" mass="15720" checksum="1B0EE13418BCBE1F" modified="2022-01-19" version="1">MGAKVPRNFRLLEELEKGEKGLGAEACSYGLSDGDDLLMSNWNGTILGPPHSAHENRIYSLNIHCGETYPDTPPTVQFVSKINLPCVDQRTGKVDPQRLPCLYQWKRENTMETILIELRRFMALPQHKKLPQPPEGSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAF6</accession>
    <name>A0A8H6FAF6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150615.1">
        <property type="nucleotide sequence ID" value="XM_037292962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAF6"/>
    <dbReference type="GeneID" id="59330448"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="29349" checksum="EAB6FAC238F6B6DB" modified="2022-01-19" version="1">MTNLVNLDSYTPPIPIPPVPPNFVFCNGAYGTGLPRREALQAGSLLPQGTLPVTYSVAEPEIDGLAANVSVLRPAYELPFLEFYGSVLISVDVASPVSISRINVVPNDIRGMAAYVANECLGRYGVGGFVTKKIQGLVDFVTDPTSNIDARMYPDSTAFLTLTVSNRQTAHAFPGDYDPQLAFFLRNAEIDALSRVQSRYHFVIANRIEKFAIAESRMRRLETEVPWWDGWLVQGNRTAIAGFQVVNVTTEGIATARRRKRVSGPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAK3</accession>
    <name>A0A8H6FAK3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150765.1">
        <property type="nucleotide sequence ID" value="XM_037293112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAK3"/>
    <dbReference type="GeneID" id="59330599"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48037">
        <property type="entry name" value="ATPASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48037:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="9"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="153" mass="16486" checksum="182C21B23F3BF4BC" modified="2022-01-19" version="1">MTDSSRLKSTIYVAGLDNAVNDQILADVFLPFGEILEVTIPKSELPSSTDPHRGFGYVEFEILGDAKEAIDNMDQSELFGRVIKVAAAKPQKDSNEGLGSKTAVWESEGYLAKHAVSEEDRIATGPGREELEAIPEDPMQGLEGLDVAGPQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB19</accession>
    <name>A0A8H6FB19_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150659.1">
        <property type="nucleotide sequence ID" value="XM_037293006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB19"/>
    <dbReference type="GeneID" id="59330493"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033745">
        <property type="term" value="F:L-methionine-(R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000614">
        <property type="entry name" value="FRMsr_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF15">
        <property type="entry name" value="FREE METHIONINE-R-SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01320">
        <property type="entry name" value="UPF0067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="10343" checksum="E184964F68EB3E4B" modified="2022-01-19" version="1">MTGLQPGQQLLPPLARLQIPPVTLVPLRVEDVHDFPGHIACDGDSRSEIVVPILAKDGKIVAIIDVDCTEVGGFDKADEEGLGSLAQLLGESCDWEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB50</accession>
    <name>A0A8H6FB50_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001868</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151335.1">
        <property type="nucleotide sequence ID" value="XM_037292796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB50"/>
    <dbReference type="GeneID" id="59330282"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058650">
        <property type="entry name" value="Msy1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323:SF14">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25886">
        <property type="entry name" value="Msy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="527"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="440"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="748"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="844"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="230"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="787"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="816"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="844"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="890"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="912"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="936"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="953"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="971"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1017"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1078"/>
            <end position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1094" mass="119244" checksum="BA4209F99A85A3AC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBE3</accession>
    <name>A0A8H6FBE3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001656</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151123.1">
        <property type="nucleotide sequence ID" value="XM_037292584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBE3"/>
    <dbReference type="GeneID" id="59330070"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF30">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="4">
        <location>
            <begin position="115"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="365" mass="40880" checksum="FC9FA8640FE9C67F" modified="2022-01-19" version="1">MGSSLQPFRPSAGEVASRQPNTTMIVLAVLPLVFTFLKACTGAVLPQYPPLPPFIALEEHFTIAEIAVQNTATPPWIVQKLLDLDGLRLEEMDQGRVTKQFLSIGAATVNLSQSQDANNVLAAAINKHPSRFGGWATIPISDPAAAADELTRSVKKLGFVGALINNHDQGKFYDNATYWPFFARAQELNVPIYLHPANPAREWASRFQGNYPQSIASSLGLSGWDWHQEVGFHILRLFNSGFFDEFPRIKIIIGHMGEMLTFQLDRVIDVGETKRGWPARQRDLRSVWNSNIWITTSGMFSLAPFACLLRQMPVDRILYSVDWPYSNNTQGLEFMEKVRRSGLVTEEQFQGIAYKNAQSVLQVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBK4</accession>
    <name>A0A8H6FBK4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001806</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151273.1">
        <property type="nucleotide sequence ID" value="XM_037292734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBK4"/>
    <dbReference type="GeneID" id="59330220"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000058">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="3">
        <location>
            <begin position="5"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="280" mass="30250" checksum="3BAE044FE4E62D18" modified="2022-01-19" version="1">MATRAANKRLTREYATIQANPPPYIVAHPSENNILEWHYILTGPPNTPYVNGQYWGTLLFPPTYPFAPPSIRMHTPSGRFQPSTRLCLSISDFHPKSFNPAWEVSTILIGLMSFMTSDEMTTGSLGASDAERKWLAARSRWWNSTGGGSVKTPVKGNTATVKGVGAVKAGDGGVKFRAEWPELDEENWNWMKEHRIDAATGKILANPEGENNTTSVACSPEVAALKRGPGGTTGLGDAGRAAREAGQSWVRRNKLMVVGIVVIGYVLMARLVGEGGAASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBT3</accession>
    <name>A0A8H6FBT3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000621</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151921.1">
        <property type="nucleotide sequence ID" value="XM_037291559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBT3"/>
    <dbReference type="GeneID" id="59329039"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012404">
        <property type="entry name" value="UCP036436"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036436">
        <property type="entry name" value="UCP036436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="48851" checksum="9D32026EAC78993E" modified="2022-01-19" version="1">MANQVMVPFLIAMMLLTGVCNTLLSKYQDLQCVANCDSSDPSKIRRFSMPVIQTAQMFIGEAACWLVVLGHYLYKRYISRPSSPLFAGGYTAVNGHDSGIDSDEEDNNEELGSSFSALEAANPVAKSLVPNADDRRELVGWRVILLALPACCDIAGTTLMNVGLLFVAASIYQMTRGALVLFVGLFSVIFLHRKLYLYQWSALFIVVLGVAVVGLAGAIAPNPQAQPEPRSTMEAVVLFARATVEDIKVTARSTEALWTIIGVLMIALAQIFTATQFVLEEWILEQYALEPLKVVGWEGLFGFIVTVIAMIILHLVVGRTEQGRNGYFDAEEGWREMTHNKAIAVSSVLIMISIGGFNFFGLSVTRTVSATARSTIDTCRTLFIWIVSLGLGWETFKWLQILGFALLVYGTFLFNDIVRPPLKSCIKKRKEEALLAEEPIEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC46</accession>
    <name>A0A8H6FC46_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152296.1">
        <property type="nucleotide sequence ID" value="XM_037291934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC46"/>
    <dbReference type="GeneID" id="59329419"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="837"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="889" mass="95409" checksum="0F1299B734295A1A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC96</accession>
    <name>A0A8H6FC96_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000650</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151949.1">
        <property type="nucleotide sequence ID" value="XM_037291587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC96"/>
    <dbReference type="GeneID" id="59329068"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000211">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="10 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="5"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6222603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="459" mass="49935" checksum="C7934FA611F63EBD" modified="2022-01-19" version="1">MGKEKMHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYYVTVIDAPGHRDFIKNMITGTSQADCAILIIAAGTGEFEAGISKDGQTREHALLAFTLGVRQLIVAINKMDTAKWSGDRYNEIIKETSNFIKKVGYNPKTVPFVPISGFNGDNMIDESPNCPWYKGWEKETKTKTTGKTLLEAIDAIDPPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKSGMVVTFAPANVTTEVKSVEMHHEQLVEGLPGDNVGFNVKNVSVKEIRRGNVAGDSKNDPPKGAESFNAQVIVLNHPGQVGAGYAPVLDCHTAHIACKFAELLEKIDRRTGKSIENAPKFVKSGDAAIVKMVPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVVKAEKSGGKVTKAAQKATGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDF0</accession>
    <name>A0A8H6FDF0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010575</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ELP1/IKA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153061.1">
        <property type="nucleotide sequence ID" value="XM_037301427.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338965"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056167">
        <property type="entry name" value="A-sol_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056164">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056165">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006849">
        <property type="entry name" value="Elp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056169">
        <property type="entry name" value="HB_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056166">
        <property type="entry name" value="TPR_ELP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23925">
        <property type="entry name" value="A-sol_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04762">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23797">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23936">
        <property type="entry name" value="HB_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23878">
        <property type="entry name" value="TPR_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017233">
        <property type="entry name" value="IKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="ELP1 first N-terminal beta-propeller" evidence="7">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 N-terminal second beta-propeller" evidence="8">
        <location>
            <begin position="399"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 alpha-solenoid" evidence="10">
        <location>
            <begin position="718"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 TPR" evidence="9">
        <location>
            <begin position="930"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 three-helical bundle" evidence="11">
        <location>
            <begin position="1098"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1186"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1288"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1288"/>
            <end position="1298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1329" mass="148937" checksum="FC5D248098D97035" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDK0</accession>
    <name>A0A8H6FDK0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010513</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152999.1">
        <property type="nucleotide sequence ID" value="XM_037301365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDK0"/>
    <dbReference type="GeneID" id="59338903"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050375">
        <property type="entry name" value="MFS_TsgA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43702">
        <property type="entry name" value="L-FUCOSE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43702:SF13">
        <property type="entry name" value="MONOSACCHARIDE TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G06630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="475"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="619"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="670"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="695"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="719"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="753"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="779"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="409"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="844"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="895" mass="96808" checksum="6A6ED567B76AF1BB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE37</accession>
    <name>A0A8H6FE37_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_011055</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153538.1">
        <property type="nucleotide sequence ID" value="XM_037301904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE37"/>
    <dbReference type="GeneID" id="59339445"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045122">
        <property type="term" value="P:aflatoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013700">
        <property type="entry name" value="AflR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF31">
        <property type="entry name" value="MONODICTYPHENONE CLUSTER TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08493">
        <property type="entry name" value="AflR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="45"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="368" mass="40825" checksum="F0B9A4A5EEE357BA" modified="2022-01-19" version="1">MDTTKPHTKYRASCDGCYLAKLKCTKERPVCPRCKKLGLTCTYSPSQRTGKRRAPKSQTSSSGSTTTTTTPTSPAPSSSGVLEWQPAASSQASTMVPTETLAQPFEKALFAPNNDPQHLPMDATMSNWPSPANTLHDFDSNFVAPWPGCLPSSDKDDPLFETSDDFVDDFISMTSLPSHHQSFNATDGALSHFLQTCNCFDSITQTLHKMHNQSRRPSNPNLEATLSCSKEVTARGEALLNCTCTEDSTLVMLFAALVAKYLSLYAAHIDLTSSSWTFLADISAASSRVTIGRYTMDAEDGERLRIEIILMELQKLNTLLMKFQGKFSSSEMGYESHTYETMVAFLNMRLREAMDKLQRQKQRYQPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEG0</accession>
    <name>A0A8H6FEG0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="15">PARP</shortName>
            <ecNumber evidence="15">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_009487</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ARTD/PARP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154196.1">
        <property type="nucleotide sequence ID" value="XM_037300348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEG0"/>
    <dbReference type="GeneID" id="59337882"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01437">
        <property type="entry name" value="parp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07997">
        <property type="entry name" value="WGR_PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.142.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.140.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.140.10">
        <property type="entry name" value="WGR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF60">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BRCT" evidence="17">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="WGR" evidence="20">
        <location>
            <begin position="179"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="PARP alpha-helical" evidence="19">
        <location>
            <begin position="325"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="18">
        <location>
            <begin position="460"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="89"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="286"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="287"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6225487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="701" mass="77649" checksum="ED9D3C035ACA34E8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEL0</accession>
    <name>A0A8H6FEL0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154331.1">
        <property type="nucleotide sequence ID" value="XM_037300483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEL0"/>
    <dbReference type="GeneID" id="59338017"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034503493" evidence="1">
        <location>
            <begin position="21"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="263" mass="26653" checksum="3E85E798D61AF30D" modified="2022-01-19" version="1">MQSKLVISVALFSAVAFAQSNDFNIDTAEETKVIGELEGFVTTLEANPTFQADVAALMTALPSSVVQEAEQDPEALIDQIGSSSEVPAWVSAIPTPVVESLETLIAKPIKAVVDVEGYIEQLVEEPEVASAVSVLMTAVPTSVQQAFESNPAGFLENIVTATALPSWVTDIPAPLQSDLGSIVNEALSIIDKDLEGGANATGTAGGFMPSSGYAKATGTGGVFGYNGTNGGPSGSPIAYTGAAAPMKTAAAGMAALMVGAGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF16</accession>
    <name>A0A8H6FF16_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154178.1">
        <property type="nucleotide sequence ID" value="XM_037300330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF16"/>
    <dbReference type="GeneID" id="59337864"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="43"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="332" mass="36905" checksum="935F4FA13C51E588" modified="2022-01-19" version="1">MAVPLQAVAIPKEITATTVILPHKPTAAIACSYVSGTDPAVLVVFLNGLMTDKSSWTPVISGIIHQRKSLASPEFPSMLAYDRYGVGMTEVRDPLDQGREQGHGHDCKDAAEDLHYLIQHFSQGLKLLSGEKLRVVLVANSIGCAIARLYAERYLVAAVLFLDSIMANSDFDFWPDPDAPGFDKRELPDDVTVEVLREQRAKFAAIFRPDVVNREGLSRRSLARLLPHSDGPMLGNQSGKPWVTVVGHDFEAFANESLRTMGTPIGLSMRYMNPMWHKYNQGLVHVTDREKSKGPIVAKGCGHFIQRDDPDFVIRETLDLVDKVRMQQSVVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFJ6</accession>
    <name>A0A8H6FFJ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PLC-like phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154834.1">
        <property type="nucleotide sequence ID" value="XM_037300008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFJ6"/>
    <dbReference type="GeneID" id="59337542"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF146">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26146">
        <property type="entry name" value="PI-PLC_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034308247" description="PLC-like phosphodiesterase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="34720" checksum="9C112D61D9D6BFB8" modified="2022-01-19" version="1">MRYPLSPISFIFSLFPLSAYAQALCNGHAELCSRQYSNITQIGTHDSAFVGTMPTDNQEVNITAQLDAGIRFLQAQSHLDGSGSLNLCHTTCMEKDAGPLSDYLTDLKIWLDANPNEVVTLLLTNGDNVNISLFDTAFTISNLNKYTYTPPTSPLGLSAWPTLNELIGNNTRLIAFLDTGAAPSVHYILDEFTYFFETPFDVTDPKFAECTLDRPKGATADGRMYIVNHFLDLSIGSVDIPDDSADDTTNAATGVGSIGAQVGICEGLYGRAPVGVLVDNFDRGNVFAAQDALNGLGGPIKGGGGGGDDGGVGGVISKGINDLKDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG39</accession>
    <name>A0A8H6FG39_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155565.1">
        <property type="nucleotide sequence ID" value="XM_037299564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG39"/>
    <dbReference type="GeneID" id="59337095"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47800">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47800:SF5">
        <property type="entry name" value="FER-1-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="47"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="569" mass="64148" checksum="B80B1A38E2EADBD7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGF8</accession>
    <name>A0A8H6FGF8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ABC transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_008380</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155247.1">
        <property type="nucleotide sequence ID" value="XM_037299246.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336776"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18596">
        <property type="entry name" value="ABC_6TM_VMR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18604">
        <property type="entry name" value="ABC_6TM_VMR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000825">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000013">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000610">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034060915" description="ABC transporter" evidence="13">
        <location>
            <begin position="27"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="849"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="922"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1001"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1024"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1113"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="158"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="523"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="862"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="1197"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="276"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1318"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1318"/>
            <end position="1332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6226939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1484" mass="163360" checksum="9CD6CC94B77ABDDE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI96</accession>
    <name>A0A8H6FI96_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156672.1">
        <property type="nucleotide sequence ID" value="XM_037298037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI96"/>
    <dbReference type="GeneID" id="59335544"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051150">
        <property type="entry name" value="SWT21/TCAB1_mRNA_Telomere"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13211">
        <property type="entry name" value="TELOMERASE CAJAL BODY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13211:SF0">
        <property type="entry name" value="TELOMERASE CAJAL BODY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="442" mass="47350" checksum="0C22814CC1C59593" modified="2022-01-19" version="1">MTSAADENHPSQPRLVGTTADLYQGSITETALKEATLLSEYPEAGLSPTKRSGTQNECNYFKSAQWSPDGTTILTSSADNTLRSFVLPADLLTPPHPQKLRPYALHLSPEPLYAATFHPSYSLHEPSTCLTLASPRALPIRLFSPFTADRILASYPLVSPTTEAWIAPHSLLFSPQEASTFFAGSESCVSVFDVNRAGDEPMTRMHTTPSRRGGAAGRGQGMKGIVSALGMSSEGMLAAGTYSRWVGLYDGFGRGGDAGVFSAGAEDEVERAGGITQVIWSVCGRYLCVVERASDGVGIWDIRGTGKRLAWLRGRNARTQQRLSVEVMGGEVWAGALDGVVRVWEGLGMAEGVRDPCWEFKAHEDTVSSTTLHPSGSVLATCSGQRHSFPSTREVEDFSDIEFDSDSSSPRSSSSASSLSSTSASSQRPPQATDNSLNLWAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIG3</accession>
    <name>A0A8H6FIG3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156758.1">
        <property type="nucleotide sequence ID" value="XM_037298123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIG3"/>
    <dbReference type="GeneID" id="59335630"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034551074" evidence="2">
        <location>
            <begin position="23"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="338" mass="35359" checksum="8DAE941E346EF9AC" modified="2022-01-19" version="1">MHPSPQLLALAASALLSSTTSASPLQPSATATYTSSSPLRTKSVDCSNVSTGLAPACWFTLNMTTYLNNWVIMSPYGGELATGETPGSTNPSPASSSAQASGDPSADDVTTGTNSSSYFRKRIVARTAAAAAPASGQCEGGKPFSTCFLRLQQGYGSRKFNCSKINTGGANGTCPAPRPAYYANNPQAFYAVWNFYAINAYISTFHRALHTLARSSPAVISTAATEAAPKISYFQSANQTLSIDVALLDLLVRSTGGHELPGGQNTPFLTLLEGDPSKVTYGEATPPAALAVLMQGRLEHVLREVMRDERVFVEVAGNGAFSTRGLLSVEGLVRVLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ11</accession>
    <name>A0A8H6FJ11_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_007493</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM U1 A/B'' family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157019.1">
        <property type="nucleotide sequence ID" value="XM_037298384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ11"/>
    <dbReference type="GeneID" id="59335892"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12246">
        <property type="entry name" value="RRM1_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12247">
        <property type="entry name" value="RRM2_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000039">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000029">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="15"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="180"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="119"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6229377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="255" mass="27829" checksum="7851F7F3FD752322" modified="2022-01-19" version="1">MAAVAAHAGVTNPSNPTCVKNLEERIKLDQLKEALTEIFSEYGNVIELVAKTNLKAKGQAFVVFDETEAAERAIKEVQGFELFDKPMLLDYARTKSDATVQREGPPEAFEAHRRRRLAEKERKQAYESAEAQKKLKRPAAGAPPEAGTARPVKASRGAGLKSSNPAAGAVIPEEYLPPNKILFVQNLPEDYGVDGLTAIFGRFEGFREVRLVPGRRGIAFVEYEAEAGAISAKENTAGMALGEEGKVVKVVYQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKH3</accession>
    <name>A0A8H6FKH3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyridoxal kinase</fullName>
            <ecNumber evidence="3">2.7.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_004537</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157455.1">
        <property type="nucleotide sequence ID" value="XM_037295454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKH3"/>
    <dbReference type="GeneID" id="59332945"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026859">
        <property type="entry name" value="Myosin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12632">
        <property type="entry name" value="Vezatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin-binding" evidence="14">
        <location>
            <begin position="162"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="13">
        <location>
            <begin position="727"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="498"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="956"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="505"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="541"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="982"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1000"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1017"/>
            <end position="1026"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6230198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1026" mass="113933" checksum="9BC2D8E640EF1EA5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKM2</accession>
    <name>A0A8H6FKM2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit TIM9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 15">mitochondrial import inner membrane translocase subunit Tim9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_004613</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="14">Heterohexamer; composed of 3 copies of TIM9 and 3 copies of TIM10, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157530.1">
        <property type="nucleotide sequence ID" value="XM_037295529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKM2"/>
    <dbReference type="GeneID" id="59333020"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000008">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="16">
        <location>
            <begin position="86"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6230273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="160" mass="18293" checksum="A654541873B541A1" modified="2022-01-19" version="1">MAQGTAPAVEREEGSRAFGWYSQVKLRGDLQLTGANAQGHLVETTEWMGEYRNGKYYKVSKSTGHLTDIALSTRLNAQEQRELQGRMEKKQMKEFMTMYSRLVQRCFDDCVNDFTTKSLQSKEEGCVMKCVDKYLKGSERLGQRFQEQNVAMQSGQLPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL71</accession>
    <name>A0A8H6FL71_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Laccase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004884</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157797.1">
        <property type="nucleotide sequence ID" value="XM_037295796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL71"/>
    <dbReference type="GeneID" id="59333290"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13877">
        <property type="entry name" value="CuRO_2_Fet3p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13899">
        <property type="entry name" value="CuRO_3_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000071">
        <property type="entry name" value="Conidial pigment biosynthesis oxidase Abr1/brown 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044130">
        <property type="entry name" value="CuRO_2_Fet3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF361">
        <property type="entry name" value="IRON TRANSPORT MULTICOPPER OXIDASE FET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034441538" description="Laccase" evidence="6">
        <location>
            <begin position="18"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="9">
        <location>
            <begin position="26"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="150"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="370"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6230540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="62382" checksum="4A2444CC851F0DE0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYC1</accession>
    <name>A0A8H6KYC1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007209</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156737.1">
        <property type="nucleotide sequence ID" value="XM_037298102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYC1"/>
    <dbReference type="GeneID" id="59335609"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="487"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="523"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="585" mass="64379" checksum="72B558E0F3799C66" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYD1</accession>
    <name>A0A8H6KYD1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156752.1">
        <property type="nucleotide sequence ID" value="XM_037298117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYD1"/>
    <dbReference type="GeneID" id="59335624"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="436"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="436"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAF6229110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="52579" checksum="C7A35E7D01135495" modified="2022-01-19" version="1">MAVTATPSQVNAMMITERVTSIFSLLGILFILATFLLGRGFDKPINRLFFFASFSNLGMNIAALIAEDGVSAGQNSSLCQFQAFAIQWYVETKVAMLFVAPNVQCRFLGVDTLWALCMGFNVYLALFRGWTSERMRRQEWKYFLACYGCSFIPALVYLFVNTKSRGKIYGPALFREAIVDAMLSSGVGCPRNGTFSAWQPSTASCGKSDSPQPTHAPLISPQPRVALLFAWIIYLMAFRKVWNNRHQLQGFFNPFNENPFEGTVTTEVDVTTYARRDSALKPTGHTTTATDADPAIPGTEPAQTAGFDPYSVNVEAGPPAPPHRHHRSDPSSASAAPDLLRVRALTRTAALRETNPEAWLYARVAFLFFGALLLSWVPSSANRLYALAHPARYSFGLNYAEALVLPLQGFCNALVYAVTSRSACRDLWRSVAGTGGGGGGAVGGKAASGGGKGADTRLERFAGRRTSRRLESDESSVASLRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZG3</accession>
    <name>A0A8H6KZG3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157130.1">
        <property type="nucleotide sequence ID" value="XM_037295129.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59332619"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12163">
        <property type="entry name" value="2-Hacid_dh_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43333">
        <property type="entry name" value="2-HACID_DH_C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43333:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE NAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="3">
        <location>
            <begin position="126"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="3">
        <location>
            <begin position="227"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6229873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="379" mass="41634" checksum="C82B76EDBA69DAD8" modified="2022-01-19" version="1">MGGGPDSKPEHLLVILPFKEPTEIFNRIRKNHPNIKITFKSLLFTSTPWMGEEDIPKEIYKDVTILVTLSALPPSPEACPNLRLIHFFSAGTNHIANSPIYTSTSIPLTTSSGIHGPQIAEWVILTALAHSHHYSFLHTLQKNREWDRAKNIKALQSLKDKAGQRLGVLGYGSIGRQVARVARGMGMDVIAYTASPRTTPESKHDKGFIVPGTGDPDGSIPEAWFSGLDKVSLRNFLAQDIDHLLISVPLTKETTHFIGAEEFAILGKKKNAFISNISRGQIIVQSDLIASLKAYDDNDPLDGGDGGGGLRGAALDVTDPEPLPKDDPLWEAPNCIITPHISGLGEAYVDRAFQVLEVNLDRREKGERMINVVDRKRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C621</accession>
    <name>A0A8H6C621_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006861</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146958.1">
        <property type="nucleotide sequence ID" value="XM_037297757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C621"/>
    <dbReference type="GeneID" id="59335261"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="22"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="435" mass="48764" checksum="9CFD4BF2B2848FAA" modified="2022-01-19" version="1">MPDLTVQTKMSSTGPKKRILYILVMGLTGAGKSTFISVVTGNEDIPIGAPSDLDGVTQEVQDYILYYHHKGCRYEIHLIDSPGFDDGTLNDAQVLSEIATYINTSYKLKQRLAGVLYLHDITKAKVGGVGKINLRMLENLIGMEKCNNCTLVTTKWGCTNLQDEEKREKTLSVEKKFFGAMLQNAQQAKMKRFEPKTRKSALDIIIPYLENEFTPHISRQMVDPRGPKLPLGETEAGKVVADNLEKLSQTKQEQKMVQHAQEILSQSYDESLFEEFKQKRKVLRRKIHMQQSGRWIMRTAIVGGAIVATVLTLGPGASAFALEPVYEKAVRGQRKAEKKAKVDLKADFIEKSKNANQLRQTNPDWLWDNKVKRLQDLEKSYSLKSGSSDSDISKVVRLGEKVGFARSEDSEAPLHAADWECSDSSDSSESDAEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6H6</accession>
    <name>A0A8H6C6H6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147159.1">
        <property type="nucleotide sequence ID" value="XM_037297449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6H6"/>
    <dbReference type="GeneID" id="59334952"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="197" mass="22167" checksum="BD74D194A37E9EEA" modified="2022-01-19" version="1">MAPKTRSMALQNVATIPKVRDSHRNIKLGTPKGSNSNGYQGSGTQPNPWCMSEEQIQRVCDHFANVGKAKSPWEYVRNHCVQQPALLPVEMDYLVNLLASVAKAQGGRERYKGYSHLDWDDVADKFNARFSADDMARRCEPRKVTVLKCAMRGNYQKVRIALLRERCREAHEGADNAGIYGSLTAEQLEHCLIHGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6M3</accession>
    <name>A0A8H6C6M3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006319</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147342.1">
        <property type="nucleotide sequence ID" value="XM_037297217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6M3"/>
    <dbReference type="GeneID" id="59334720"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF129">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="29"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6217907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="264" mass="30018" checksum="BDFCBC4053E69E54" modified="2022-01-19" version="1">MIVALRLYTRVFITRNFWLDDAAIALTQFFIALATCKISICLFLLRLSHFNRLKHILYGLIGFLVVTHLTLFFLTVFQCSPVDKVLNPADPGNCFSEQMVMNILIAQRVFSFITDFICAAFPIVLLRNLKIKKQSKIALCCLMGLGIITGGIAIVRTATAWQIKSEDLSWVGVPNAMTRIFEVNIGNIAACVPRLKTFSRYVHARITGRDPHEMLRRKASPSSSHPNWYSKRFWLRRPSPAYTKSERELRQVPAEARRALPCVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6W5</accession>
    <name>A0A8H6C6W5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147425.1">
        <property type="nucleotide sequence ID" value="XM_037297300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6W5"/>
    <dbReference type="GeneID" id="59334803"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034809530" evidence="1">
        <location>
            <begin position="22"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="286" mass="31494" checksum="2FD67DE80D8E8E1E" modified="2022-01-19" version="1">MVFISFLHVSLFFISLKDSFAMNRIHNPNTTFLTSNLTLSSLPITPFGANTSLTLPTVHCDGVTYKSNLLSASCADAYRTIPRTTKLLTFGDREDGDFEVPLPYRWVSSDGLCIFEIAKIADTPVAYATLRDFERAAFMLLTTCFADGRTEGGIAGNLGPGVLSLGLIMTSNRQIVQCTGPTGPPRDSCYSIIATMPALKEQQLFSRSPGGHVNVRIPDSFESQDGRCIVTLDTIGREDIATWYEVWAAVMAVDDMCMRFGRVGKAMEIGAFRRLFLEINFHVGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C769</accession>
    <name>A0A8H6C769_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_006057</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147534.1">
        <property type="nucleotide sequence ID" value="XM_037296960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C769"/>
    <dbReference type="GeneID" id="59334462"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF155">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="37"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="382"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="407" mass="45190" checksum="4DDB93058834EC3D" modified="2022-01-19" version="1">MSSSGPPPPGGDYNRAGEVHAFTWTLEILSLMFVVGRMYSRTKLTRNVWWDDWCICFALVLDFVVAIMWSVYAARGYARHLYYLTPAQISNALELNTISRSLCMFSIAVGKISVAFLIERIAGPNDWRKWLLRGISISVFISAVITFTLFYVQCQPARALWDKGMIKEGTGSCWNPIPVNTWDLVIASYWAFLDFALAFIPVDIVWKLHLSTQKKLLLALLLGMGVFAGVCAAVKTSKVPITVKAKTDITWQTIELLMWNGIEMNVIIIAACIPTLRPIFLILLKRPGADNFRASVRERGRSSYYYRTSDSEESKKTTTGSSAPSKAFDKRASRAITGSTEAINKKSSNDGGVVLQLESREVGGRDEEIEEGDWGHAHGFGIPMTGIGSDQDAAEMDSGRLREDSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7J0</accession>
    <name>A0A8H6C7J0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">cAMP-dependent protein kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_005749</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8 9">Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147837.1">
        <property type="nucleotide sequence ID" value="XM_037296656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7J0"/>
    <dbReference type="GeneID" id="59334154"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000039">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 9 10" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="135"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="267"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6218402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="396" mass="42556" checksum="762AC39913DD0980" modified="2022-01-19" version="1">MAESTFPGGSGPFGGFNSGRSPSMANVHEDEESDTITSPTNTASAAAAPGSPFANSSNFSFNASSGHSQPPSAFRPPGPDDQGFPNNYNMTRRTSVSAESLTPTHHQDSNWKAPYHQKTEDQLSRLRASVSSNFLFSHLDDDSANLVLGALVEKPIPAKDIKVITQGDAGDYFYVVERGAFDIYVNPAGHVTPGPDGMGQKVNTIGPGGSFGELALMYNAPRAATVVSTEKESTLWSLDRVTFRRILMDSAFQRRRMYESFLEEVPLLSTLTLYERSKIADALDTQKFLPGATIIREGDPGEAFFILESGEAEVYKSDSGDTVVKRYKKGDYFGELALLDDKPRAASVVAAKHGGEVKVAYLGKQGFQRLLGPVEGIMRRNDYSGGGGEGSAPATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7K6</accession>
    <name>A0A8H6C7K6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147544.1">
        <property type="nucleotide sequence ID" value="XM_037296970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7K6"/>
    <dbReference type="GeneID" id="59334472"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="205" mass="22624" checksum="A68EC2724362C136" modified="2022-01-19" version="1">MSKLLSVHRCFLFIMLLIVGARLATMIPFLLLLVILPLVMCDPLQLDLLSTSNTSWTASHNASTLNATRLTTDNDCFDDILGKIFPLTKSDCEHALDTLVKGKSLVEPHYFGYQSRGVTDRLPLKAEYGTCSITFMMFDLDKRIALTYAEIYAELLGPDGVLKECLGPSVPAEDALGGQTVLGPRNLLIVDVEGRPYNAEDQKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7S3</accession>
    <name>A0A8H6C7S3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147686.1">
        <property type="nucleotide sequence ID" value="XM_037297112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7S3"/>
    <dbReference type="GeneID" id="59334614"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034987474" evidence="1">
        <location>
            <begin position="20"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="274" mass="29678" checksum="1A2A41F41E799FB7" modified="2022-01-19" version="1">MFVILALAYVLIALPSTLASCGPIDDVTLTFYGWPDNSPPGSDNAFDCGRGKNADGNPMAGGSGTYDDPISFATATNNRNLPECAVVYVPLLHKYFRNEDDCAQCLTDWDSKGEYHIDLWTGSNTAGGGQDQINCEDNLPGGSQTIILDPPKSLPVDTTPLYDQPSNQCHTTTYSISDSSSLCAGGSKGGSPAPKTTKRTPAIQKSVKRVEMETAGSTLGADETICEEPEQPPLYFVLFFLRVIATTFYRREIKDQESKAYDEYHIILPSPIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C858</accession>
    <name>A0A8H6C858_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the Golgi-associated retrograde protein (GARP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS51 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148102.1">
        <property type="nucleotide sequence ID" value="XM_037296921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C858"/>
    <dbReference type="GeneID" id="59334423"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014812">
        <property type="entry name" value="Vps51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU368010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="248" mass="27204" checksum="93BCC65847A991A2" modified="2022-01-19" version="1">MATVTSPRPSNPSGSLASTPTTSRTTSPAPTRRQHRAALRDYYNLKTAAPNEAPSKPHVSEPDSPSSELDASDFDADAYVKSVLAGHGLEGVLRVEAGLVNEIKGLDGERKALVYDNYSKLITATDTIRRMRDNMDPLTPTTSTLSPAVAHIAETARGLVARAGNGNGGKEADAGEERREVEKVRSRQRDTVRWVLGTPRRLGLMIDDGRVEDAKRDWEEIQGILRRWRDVAGVEELRDECEKTMSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8C4</accession>
    <name>A0A8H6C8C4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_005282</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148175.1">
        <property type="nucleotide sequence ID" value="XM_037296193.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333688"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001010">
        <property type="entry name" value="ABC multidrug transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="547"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="582"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="622"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="660"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="689"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="795"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1213"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1242"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1292"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1321"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1348"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1465"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1527"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="178"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="872"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="82"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1580"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1658"/>
            <end position="1691"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1611"/>
            <end position="1625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1661"/>
            <end position="1680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6218740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1705" mass="189118" checksum="DF8AF806AF1F9250" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8W0</accession>
    <name>A0A8H6C8W0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mid2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148434.1">
        <property type="nucleotide sequence ID" value="XM_037296452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8W0"/>
    <dbReference type="GeneID" id="59333949"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="33330" checksum="3132330D602DFD3F" modified="2022-01-19" version="1">MRAPNRIYSYSRTSRIAKSTALIATLLTRTCAATLPAPPSPADKWLYPDVNTLPTYNYLDTVNASWTSNFVAPYLLLRCQHPNDTAHYAYPYNQSVPATGSALVPLDDGNAWVCHFEVSDLSASSATPPTFISNDFNIVLGVGQRPVLWTNAQSRGETAASSATSNPTAASPSSSRISTSSTTTSSVATTHTPLSSATSTPTTAPTTSSTIASSSATTPTPTQPSTPPTPSSTTSGLSKSKKIAIIVGSTMGALLLLVVAVAVLFILRTRRSERNAVVYRSGPGNGYGSKEVPGLEAPRERWKGLEIPSVQPQGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9M9</accession>
    <name>A0A8H6C9M9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Thioredoxin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004054</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative mitochondrial redox protein which could be involved in the reduction of small toxic molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMP46 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149020.1">
        <property type="nucleotide sequence ID" value="XM_037294973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9M9"/>
    <dbReference type="GeneID" id="59332463"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012882">
        <property type="entry name" value="Fmp46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28071">
        <property type="entry name" value="REDOX PROTEIN FMP46, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28071:SF1">
        <property type="entry name" value="REDOX PROTEIN FMP46, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07955">
        <property type="entry name" value="DUF1687"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="39"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="16150" checksum="C939E88A1BFF7F94" modified="2022-01-19" version="1">MFRFHKSLDVITLFHKPSVPASLRVLTLLKQISAHASETATEDQATDHTHQNKLQRTEFELNITEDPPTGDQMRTILEYLGAWNAGRLVEGAKDSGDAIRKLGENPKTFKPPVTVDWNNGNVVVGDDESEILRMISQLPKETGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9N1</accession>
    <name>A0A8H6C9N1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prion-inhibition and propagation HeLo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003898</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148867.1">
        <property type="nucleotide sequence ID" value="XM_037294820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9N1"/>
    <dbReference type="GeneID" id="59332309"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1020">
        <property type="entry name" value="Prion-inhibition and propagation, HeLo domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029498">
        <property type="entry name" value="HeLo_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038305">
        <property type="entry name" value="HeLo_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542">
        <property type="entry name" value="HELO DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14479">
        <property type="entry name" value="HeLo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prion-inhibition and propagation HeLo" evidence="2">
        <location>
            <begin position="11"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="549" mass="62308" checksum="EEC54029C36BA653" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9X4</accession>
    <name>A0A8H6C9X4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004081</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149047.1">
        <property type="nucleotide sequence ID" value="XM_037295000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9X4"/>
    <dbReference type="GeneID" id="59332490"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23813">
        <property type="entry name" value="UBCc_UBE2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000020">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="3"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="150" mass="17041" checksum="BD7432E338934497" modified="2022-01-19" version="1">MAALPKRIIKETERLMAEPVPGISAVPHEDNLRYFDVKIHGPSQSPYEAGIFKLELFLPDDYPMTPPKIRFLTKIYHPNIDKLGRICLDVLKSNWSPALQIRTILLSIQALLGAPNPDDPLANDVAQRWKEDEPAAIQTAREWTRQYAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB91</accession>
    <name>A0A8H6CB91_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003376</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149679.1">
        <property type="nucleotide sequence ID" value="XM_037294298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB91"/>
    <dbReference type="GeneID" id="59331787"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="119" mass="13480" checksum="1C1903A7DB98E56D" modified="2022-01-19" version="1">MWRDNTDVAVAITVRRAIAKLAKPIQVSVLRLLPGTEGGEIKCCITMFDIENYEITSQHGKELLKQIEDEISRIKPRNQDSYGNREYELSELLDEKVRALLVNLQLSVREGVISIIPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBB0</accession>
    <name>A0A8H6CBB0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003404</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149707.1">
        <property type="nucleotide sequence ID" value="XM_037294326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBB0"/>
    <dbReference type="GeneID" id="59331815"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050197">
        <property type="entry name" value="Aldolase_class_II_sugar_metab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22789:SF0">
        <property type="entry name" value="3-OXO-TETRONATE 4-PHOSPHATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22789">
        <property type="entry name" value="FUCULOSE PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="3">
        <location>
            <begin position="25"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="288" mass="31671" checksum="08BDFEC3C8A9AC67" modified="2022-01-19" version="1">MSPDSELPSTPIARMVAVNLTQFLSTLITANHILDYNQIVDGLGHVSVRNPNNNATFFMTGDPPPALVQSLADLNEYYISDGSAVTPSADHAAQSPYSERFIHQGILKRYPSQISVVHSHDLKVIPFGISEVPFKPTYHMAGFVGEEVPVFDIANYYLPNDTQNMLISSTRFGDALADTFSTLANNVTKNNTEPDYTLVLQRGHGFATVGTSIEESVYRAVYTAWNAETQASALEIQNAYGASAETLKYLTPREAADCVPMNQGSYTKAWPLWQAQVEADPLYKNDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBZ2</accession>
    <name>A0A8H6CBZ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150190.1">
        <property type="nucleotide sequence ID" value="XM_037294112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBZ2"/>
    <dbReference type="GeneID" id="59331600"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF107">
        <property type="entry name" value="GLYCOSYL TRANSFERASE CAP10 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="1">
        <location>
            <begin position="73"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="340" mass="39099" checksum="8B1CB5FD24FF675C" modified="2022-01-19" version="1">MVARHTSNPIRADSLDAEMTGDHAVTGPVKAMIFKGELFVIEDKGMTDYYGTRLFATLDAIHRALVSFPDRAQLPSCEFIISWGDKPARGAPVWGYTKNDTPEYYDTWLMPDFGFFSWPEPKTGAYTEIRRAIRRVEKETPFEKKVPKLVWRGAPLIEDRVKFLDYTQQKAWADVRVINWEDGNDLEKNYLTLAEHCRYMFIAHVSGLSWSGQGKYTRNCHSVLISHELEWREIYDSALVYSGPEQNAVRVADDWSDLDATMETFLANTTAAKTIADNAKDSLHDRYLTPAAEACYWRRLIQGYASVSFDPELYEADEQTLRGVPYESFTLLRKVHWDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCP2</accession>
    <name>A0A8H6CCP2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002703</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150457.1">
        <property type="nucleotide sequence ID" value="XM_037293628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCP2"/>
    <dbReference type="GeneID" id="59331115"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000552">
        <property type="entry name" value="GPN-loop GTPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="300" mass="33183" checksum="258F2BDA918C4ECC" modified="2022-01-19" version="1">MSKFGVVVMGPAGAGKTTFCSALIQHLRLNKRSCFYVNLDPAAEDFSYAPDLDIKELISLEDAMEEMGLGPNGGLIYCFEFLLDNLDFLTEAIDPLTDEYLIIIDMPGQIELYTHIPIVPTLIKHLTRGGALNVNLCAAYLLESTFVIDRAKFFAGTLSAMSAMIMLELPHVNVLSKMDLVKDSVSRKELKRFIDPDNSLLDDDPTDSKAALISSQNKSADPSSADSVMAGGSFKRLNRAVAQLIDDFSMVSFLQLDVQNEDSVGAILSYIDDAIQFHEAQEPREPNDEVEYDYQDAEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD26</accession>
    <name>A0A8H6CD26_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EKC/KEOPS complex subunit BUD32</fullName>
            <ecNumber evidence="7">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25 26">Atypical Serine/threonine protein kinase BUD32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">EKC/KEOPS complex subunit bud32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">HO133_002087</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. BUD32 has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit KAE1. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="31">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150667.1">
        <property type="nucleotide sequence ID" value="XM_037293014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD26"/>
    <dbReference type="GeneID" id="59330501"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000603">
        <property type="entry name" value="EKC/KEOPS complex subunit bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000845">
        <property type="entry name" value="Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06293">
        <property type="entry name" value="Kdo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="22" id="KW-0010">Activator</keyword>
    <keyword evidence="19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="20" id="KW-0779">Telomere</keyword>
    <keyword evidence="23" id="KW-0804">Transcription</keyword>
    <keyword evidence="21" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="30">
        <location>
            <begin position="22"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="171"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="29">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00019973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00030980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00033194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="KAF6221232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="283" mass="30887" checksum="4044102AFBFEA902" modified="2022-01-19" version="1">MEITNNDPPQPHPLPPPFTHTAPAPTLLTQGAEALLYRTHFLLPSHPCALKHRPSKPYRHPVLDKRLTKHRILSEARTLVKCRREGVSVPGVLALDAEGCGDAGSGGWMMMEWVEGRTVKEVVVEAGKRIKERGGGGVWEEGLRALMGKIGRALGRMHEVGVVHGDLTTSNMMLRPSPTSLQNGTTFTNGAEGDGDDAKSAGESLEGEIVLIDFGLAAQSLQDEDKAVDLYVLERAFGSTHPEAEEGFQEVLRVYGESYKGAKVVLKRLEDVRLRGRKRSMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDL3</accession>
    <name>A0A8H6CDL3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_001623</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151090.1">
        <property type="nucleotide sequence ID" value="XM_037292551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDL3"/>
    <dbReference type="GeneID" id="59330037"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000005">
        <property type="entry name" value="TfdA family taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF10">
        <property type="entry name" value="TAUD_TFDA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="14">
        <location>
            <begin position="77"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="15">
        <location>
            <begin position="527"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="452"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="846"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="682"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="733"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="832"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="529"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="584"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
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        </ligand>
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        <location>
            <position position="733"/>
        </location>
        <ligand>
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    <feature type="binding site" evidence="11">
        <location>
            <position position="735"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
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        </ligand>
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        <location>
            <position position="831"/>
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        <ligand>
            <name>Mg(2+)</name>
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            <position position="832"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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            <position position="801"/>
        </location>
    </feature>
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            <position position="832"/>
        </location>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
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        <source>
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        </source>
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        <source>
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        </source>
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        </source>
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        </source>
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        </source>
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        <source>
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        </source>
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        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6221655.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="869" mass="96526" checksum="16EC0B41362F9E68" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDQ1</accession>
    <name>A0A8H6CDQ1_9LECA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">HO133_002354</name>
    </gene>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6221498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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            <begin position="1"/>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="796"/>
            <end position="854"/>
        </location>
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        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
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        <location>
            <begin position="265"/>
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        </location>
    </feature>
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        <location>
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            <end position="564"/>
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        <location>
            <begin position="722"/>
            <end position="738"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6221498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8H6CDT5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="1 2">
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            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150844.1">
        <property type="nucleotide sequence ID" value="XM_037293191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDT5"/>
    <dbReference type="GeneID" id="59330678"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="60" mass="6131" checksum="2E381ED80BE21A34" modified="2022-01-19" version="1">MSDMSNTGSSENSAQTRFAAAAKKKCLASIVSFAGGDLCSQVAISRTRCPVEAGCIGESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE42</accession>
    <name>A0A8H6CE42_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-hydroxyacyl-CoA dehydrogenase NAD binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001816</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151283.1">
        <property type="nucleotide sequence ID" value="XM_037292744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE42"/>
    <dbReference type="GeneID" id="59330230"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="543"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="593"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="629"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="656"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="728"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="788"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="820"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="7">
        <location>
            <begin position="38"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="6">
        <location>
            <begin position="228"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="885"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="897"/>
            <end position="910"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="910" mass="97975" checksum="06F8FA4F9C0D78B1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE95</accession>
    <name>A0A8H6CE95_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151358.1">
        <property type="nucleotide sequence ID" value="XM_037292819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE95"/>
    <dbReference type="GeneID" id="59330305"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="223" mass="24441" checksum="6AC12BAE769F434A" modified="2022-01-19" version="1">MPLEAARGTHPTTFVRDITQPEEFKVYTDYDAYAHSTGEDENKVNLAEGEADSITVSAGNDQNPPSMHPAASVLEYDQANTYNYLSGRLPQTADSPLISPTPPTPTSGPSTHAPAQLPLPQFQPASMQVGNPTQMPATPAPIHPLPIPWHPLHETIPGMTLGWTTCNTDHRRHKRCDHGLDGRIDPVLAAMPHKVMANRKSWRTKKSKCTKMGRSEGTRQKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEK5</accession>
    <name>A0A8H6CEK5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF833-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151256.1">
        <property type="nucleotide sequence ID" value="XM_037292717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEK5"/>
    <dbReference type="GeneID" id="59330203"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008551">
        <property type="entry name" value="TANGO2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985">
        <property type="entry name" value="SER/THR-RICH PROTEIN T10 IN DGCR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985:SF8">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05742">
        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="36009" checksum="5D2FC43A35DA0834" modified="2022-01-19" version="1">MCIALFSTAHPDYALILINNRDEFLARPTAPANWWASPNENVLGGRDLQRKEQGTWLGITKQGRVAVLTNFREEGAIKPEARSRGAMVNAFLTQPQSLDGTERFIHDLVEDEGLKGVGGFSLVCGKVGEPLAVISNRTPNVEDTVWIAKDKDETIGLSNAAFGNRSWPKVVRGEALLFSAIKQNLSEQRSQASFTEHLFRILGEDILPRKPKDYPWDSYVQELRKSIFIPAIGGEGAEESRAEDLAAAKNDQHVNVQNVTKAAEQEHEFDGVYGTQKQTVVLVDRRGRVNFVERTLYDATGKATVAPDRDRAFQFDIEGWKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEL4</accession>
    <name>A0A8H6CEL4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151271.1">
        <property type="nucleotide sequence ID" value="XM_037292732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEL4"/>
    <dbReference type="GeneID" id="59330218"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="850" mass="95049" checksum="6C4FA9026202E32C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEV9</accession>
    <name>A0A8H6CEV9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151356.1">
        <property type="nucleotide sequence ID" value="XM_037292817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEV9"/>
    <dbReference type="GeneID" id="59330303"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="160" mass="17833" checksum="51F407AC6C9960AD" modified="2022-01-19" version="1">MAGGDVYAGAASRKKFLRRLEVLQSASIEGKGPPQLKSFEETDSDQQHRTEGEIDPSNHTAVLSSTKIFIRTTTKRRCGEEHGTIQFPYQPDAFREHLTAGIEVICSELLDDRQLKPRKTGIVDSAAQGTQLYPITYVAHEEIDRAEPQLGSDNDPHKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF25</accession>
    <name>A0A8H6CF25_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151640.1">
        <property type="nucleotide sequence ID" value="XM_037292221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF25"/>
    <dbReference type="GeneID" id="59329707"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="305" mass="35181" checksum="82B74F0D1FBBDAD9" modified="2022-01-19" version="1">MTNPPPTLLTLPVEIRSSIFYLALLNTPEVCAPEPPYRHSLDLSLLSTNRQVYYETRTIPLALHHFGNQYDPQVNFLSSLRLRPFQIAALKILVIESLFPGHLTHFLALGNDNGYLFGEEALGLDLLVIYAEDWIGKGARRWRYTASPEDVHYNLPMSSRWLRALCGLKGWNRLEIVLGTRDLVNEYWDYDRFMQTLFDDFRSRLGDLDEGFTIWHEGRDVPSEKIVVLRTRELGRYKQTEWWRADVGRLMDGSECVLRGSMDINEDDGGGRPGFHVQERCGVPENRKNHCTRCQPGCGCYTTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFL9</accession>
    <name>A0A8H6CFL9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001402</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151751.1">
        <property type="nucleotide sequence ID" value="XM_037292332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFL9"/>
    <dbReference type="GeneID" id="59329818"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF18">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_5G12480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="282" mass="29498" checksum="35CE9CC7325047AE" modified="2022-01-19" version="1">MSSLEAANLFNVKDLVAVITGGGSGIGLMMTKTLALNGAHKVYIIGRRKEVLDAAAKESPHGNIIPLVGDVTSKDALQSIVSHIEKDVGYINVLIANSGIGGPQSSSITPETSLEDYQSALWNQSFDEYTKTFAVNTSAVFFCTVAFLKLLDAGNETGNVEQTSQVIATGSIAGFNRRVPGGYAYGQSKAATTLLMKAMATNLVPYKIRANVVAPGLFPSDLAAGIIGDGVFPKETIPAERVGTPEDMGGAILYLTSRAGAYCNGNVVLLDGGRLSLMPGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG56</accession>
    <name>A0A8H6CG56_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH:ubiquinone oxidoreductase intermediate-associated protein 30 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000968</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CIA30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152263.1">
        <property type="nucleotide sequence ID" value="XM_037291901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG56"/>
    <dbReference type="GeneID" id="59329386"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010257">
        <property type="term" value="P:NADH dehydrogenase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013857">
        <property type="entry name" value="NADH-UbQ_OxRdtase-assoc_prot30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039131">
        <property type="entry name" value="NDUFAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194:SF18">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08547">
        <property type="entry name" value="CIA30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="5">
        <location>
            <begin position="44"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="243" mass="27353" checksum="9CE32264389E1931" modified="2022-01-19" version="1">MRASSRLLAPGFTRRSLDELKRFTKIALTQEGIRVPTTPFSLLDFSDPDTVPACKTMSDADIGGFSNASLDQIPRTSSQPAHARFHGSISTDLPRNRPQVQRTGYAGWRNRDRPPTLFGKSLWDVDPYTYLALRVKSDGRKYFVNVQTESIVHTDLHQHRLHARKPGEWETVLIEINAFVRTNHGEVVEPQTEMMRQKVRTLGVSLTDRVPGPFDLAISRVWASNANEKDDELVDTGGLEEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGX7</accession>
    <name>A0A8H6CGX7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Altered inheritance of mitochondria protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152524.1">
        <property type="nucleotide sequence ID" value="XM_037291089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGX7"/>
    <dbReference type="GeneID" id="59328568"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03062">
        <property type="entry name" value="TRX_Fd_Sucrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF7">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="375" mass="40839" checksum="C29D280121C94C0D" modified="2022-01-19" version="1">MSSPLMSTHHDPQRISLIIRRLRPSFARRALTTSPPASAPPIPVPIGSQASYPIPTTPTCPPSGCACAAMPSGLDIDYGRPLDGTMPPYVQHVVVRTGRDDWGSRIEDEDGSVEGIGMNFARSLRGLVGRGGKFFDPQRPILITNSSLPAKAGPEYTSVHVFPQHVHVPKIPHTPEAHEAFVSQFLISKGAMAEKMWTKREIQKSEGDDVDQTGFPIPEEDLVAYKSRLNYAKNVSPTILICGHNSRDTRCGILGPLLRAEFNSYIGGRTQLPADDERSMGFRFSGLQPVSNVGKSRVALTSHLGGHAFAGNVVVYLPKRFKLASGKVSPLAGMGIWYGRVEPRHVWGIMEETVQRGCVVKELLRGIHEPGVIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHH7</accession>
    <name>A0A8H6CHH7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000351</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAPP small subunits family. BET5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152725.1">
        <property type="nucleotide sequence ID" value="XM_037291290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHH7"/>
    <dbReference type="GeneID" id="59328770"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14855">
        <property type="entry name" value="TRAPPC1_MUM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000013">
        <property type="entry name" value="TRAPP complex subunit, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF16">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="160" mass="18128" checksum="C83687BED07A034D" modified="2022-01-19" version="1">MVVYSFYVFDRHAECIYKKRWAPRPGSSSKAPQANGVLDVQSPFKGLSAEDDAKLVFGTIFSLRNMVRKLGGPDDNFISYRTGHYKLHYYETPTSLKFVMVTDTKTNNLRIVLHQIYVSLYVEYVIKNPLSPVEHPGGIGVNCELFELGVEQFVTASLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHQ4</accession>
    <name>A0A8H6CHQ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_000277</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat ELP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152651.1">
        <property type="nucleotide sequence ID" value="XM_037291216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHQ4"/>
    <dbReference type="GeneID" id="59328696"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000400">
        <property type="entry name" value="Elongator acetyltransferase complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037289">
        <property type="entry name" value="Elp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50960">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="54"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="203"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="286"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="392"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="657"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6223434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="755" mass="82827" checksum="C3A52F569065D19C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIS7</accession>
    <name>A0A8H6CIS7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010565</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153051.1">
        <property type="nucleotide sequence ID" value="XM_037301417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIS7"/>
    <dbReference type="GeneID" id="59338955"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="129" mass="14381" checksum="8BCA6AFD8D19C11A" modified="2022-01-19" version="1">MVYNTPLSIVPIQHAGLLADRSLGFFLQHALGNLRAIELLAAELYLPEPLLILTPIFHKPKVPGIVRIMEDLEEGTFWLRSHSIEADLKGGEELVDVLRSDSDVNMKADAMKCVRVLDVGRHDARMPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ59</accession>
    <name>A0A8H6CJ59_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_011095</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153578.1">
        <property type="nucleotide sequence ID" value="XM_037301944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ59"/>
    <dbReference type="GeneID" id="59339485"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_uS15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15, BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321:SF26">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="38600" checksum="03B6480CB147EAD4" modified="2022-01-19" version="1">MASRLPLRLQTRTAVESLWCHTPPSFLAPILASLHLHFSTTSSLGWNIRQLKKRKQVHRDEYTLRQVKARRDANLSRQAVLKEQKAKELGDPIRGIPTPFVESFDTEVPQEIKQDPSAVSLEAVKANPDTPPPSPAYASEEKHLDHGIKHEELARSLEFSRYLTRPLPPPSSTISDPDQEAQAHERWEKRDASATEAVQRIVSLTNASSKHRTKKNTERIIETFGRHNTDHILKPKGPAVVKHNPLIPPQVPTPRIGPDTGSSEVQIGILTAKIRVLADRYEGPNRNDKVNKRNLRLLLHRRQKLLAYMERKERGSERWQRMISTLGLTPACWKGEIAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJC9</accession>
    <name>A0A8H6CJC9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153334.1">
        <property type="nucleotide sequence ID" value="XM_037301700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJC9"/>
    <dbReference type="GeneID" id="59339239"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="122" mass="14718" checksum="8A1C0E8438F5EFB0" modified="2022-01-19" version="1">MSGNPSENTGDPSGHNAPPGVKKPPQWLIDVNWMTNHLAVEERDREWEKQKEAEEKRRKEEMEAERQEREKEQKRDAKWRRRNAEKEAERQDQERKQKKEAEEKRRNEKKEDGASIKQEEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK99</accession>
    <name>A0A8H6CK99_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153681.1">
        <property type="nucleotide sequence ID" value="XM_037300864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK99"/>
    <dbReference type="GeneID" id="59338400"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="124" mass="13934" checksum="36F3D5D96D5E5638" modified="2022-01-19" version="1">MENTAENTMENTAEKPSGNKATIVKNTAQLSKIPRHMLSTVPHQIITQQAENTTATWPVIPTALIWYITAITWASAGGISNDAYLDELKPSDVYSYFIIRKISLRQEILQLLRINPPHSIYLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKJ7</accession>
    <name>A0A8H6CKJ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.256</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Asparagine-linked glycosylation protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_010000</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the third and last glucose residue from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide intermediate Glc(2)Man(9)GlcNAc(2)-PP-Dol to produce Glc(3)Man(9)GlcNAc(2)-PP-Dol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate = a alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29543"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="Rhea" id="RHEA-COMP:19512"/>
            <dbReference type="Rhea" id="RHEA-COMP:19522"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:132522"/>
            <dbReference type="ChEBI" id="CHEBI:132523"/>
            <dbReference type="EC" id="2.4.1.256"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:29544"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALG10 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.256" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153673.1">
        <property type="nucleotide sequence ID" value="XM_037300856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKJ7"/>
    <dbReference type="GeneID" id="59338392"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106073">
        <property type="term" value="F:dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016900">
        <property type="entry name" value="Alg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989">
        <property type="entry name" value="ALPHA-1,2-GLUCOSYLTRANSFERASE ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989:SF10">
        <property type="entry name" value="DOL-P-GLC:GLC(2)MAN(9)GLCNAC(2)-PP-DOL ALPHA-1,2-GLUCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04922">
        <property type="entry name" value="DIE2_ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028810">
        <property type="entry name" value="Alpha1_2_glucosyltferase_Alg10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="543"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="381"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6224806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="575" mass="66481" checksum="874DD6F401C1EF39" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKS4</accession>
    <name>A0A8H6CKS4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154159.1">
        <property type="nucleotide sequence ID" value="XM_037300311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKS4"/>
    <dbReference type="GeneID" id="59337845"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="118" mass="12848" checksum="80579C1A846B2026" modified="2022-01-19" version="1">MVQTDETWTHEKGGYVNLSITGGATCDYVRINRAVGDADLGEHIAEPEDSASALNIPAVNGLSARVCDSMKVEGEYGRVSRQNKAVDTNCDDANELSEEPEPKVLAPKRGLRFWRSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKY5</accession>
    <name>A0A8H6CKY5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PABS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009555</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154264.1">
        <property type="nucleotide sequence ID" value="XM_037300416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKY5"/>
    <dbReference type="GeneID" id="59337950"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010487">
        <property type="term" value="F:thermospermine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037959">
        <property type="entry name" value="MFS_SpdSyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43317">
        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43317:SF1">
        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="359"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="601" mass="66976" checksum="0DA8A042AF524545" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLJ2</accession>
    <name>A0A8H6CLJ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ferrochelatase</fullName>
            <ecNumber evidence="12">4.98.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_009595</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>heme b + 2 H(+) = protoporphyrin IX + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.98.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ferrochelatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.98.1.1" evidence="12"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154304.1">
        <property type="nucleotide sequence ID" value="XM_037300456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLJ2"/>
    <dbReference type="GeneID" id="59337990"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1400:FF:000003">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF1">
        <property type="entry name" value="FERROCHELATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 12" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6225595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="388" mass="42730" checksum="13740000CEBE8BF8" modified="2022-01-19" version="1">MATVVPPVTQDATSSKGPTAMVFMNMGGPSTTDEVGDFLSRLFADADLIPLGRLQSYIGPLISSRRTPKIKKQYAAIGGGSPIRKWSEYQSSEMCKLLDQISPETAPHKPYVAFRYANPLTEEMYTNLLKDGFGGGKGGRAVAFTQYPQYSCSTTGSSLNELWKWRNRLEGKGATGLDSAGSITWSVIDRWPVHPGLVEAFAQNVETQLATYPEESRKDVVLLFSAHSLPMSVVNRGDTYPAEVGATVYAVMQRLGFSNPYRLCWQSQVGPSAWLGAQTSDTVQQYVKKGQTDLILIPIAFTSDHIETLFELDQEVIHEAGHPGVKRAESLNDSRVFIEALADVAVNHLRSGESCSRQMGLRCQGCKSERCLEQKKFFVSQENLLSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMN1</accession>
    <name>A0A8H6CMN1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">26S proteasome non-ATPase regulatory subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009139</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit p27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154826.1">
        <property type="nucleotide sequence ID" value="XM_037300000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMN1"/>
    <dbReference type="GeneID" id="59337534"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040815">
        <property type="entry name" value="Nas2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035269">
        <property type="entry name" value="PSMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18265">
        <property type="entry name" value="Nas2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nas2 N-terminal" evidence="5">
        <location>
            <begin position="35"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="149"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="23734" checksum="86049CB648325E89" modified="2022-01-19" version="1">MGLYMDDIHTPTVPSGPTTGQRAADGVGKDKLSLMDLIAEKTRVEEELTALGSVLDSHGVNMNTTLTTFDGYPRDDLDIAQIRTTRARIVYLRNDYKTLMSKIELGLHEHHASYQASNPSLASGSSQGPLRSGSAATDQDLIDTPFAKVNSVVPGSPADDAGLKAGDRIRKFGDVNWLNHEKLGKVAEIVQRNQGRNVVEQLGGPRSAWLPPAASLMAWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMX3</accession>
    <name>A0A8H6CMX3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009308</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures. Also a component of the nuclear pore complex.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Cytoplasmic dynein consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits which present intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the dynein light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154995.1">
        <property type="nucleotide sequence ID" value="XM_037300169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMX3"/>
    <dbReference type="GeneID" id="59337703"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043935">
        <property type="term" value="P:sexual sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21452">
        <property type="entry name" value="DLC-like_DYNLL1_DYNLL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000001">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019763">
        <property type="entry name" value="Dynein_light_1/2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01239">
        <property type="entry name" value="DYNEIN_LIGHT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 11" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6226442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="94" mass="10881" checksum="C41B55F4597839A1" modified="2022-01-19" version="1">MATEKKEKLDAQVKSADMSEEMQRQAVEVAEEAMSKFQVEKDIAQYIKKEFDSRAGATWHCIVGRNFGSFVTHETKHFIYFYLGHCAILLFKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMY5</accession>
    <name>A0A8H6CMY5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009365</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155052.1">
        <property type="nucleotide sequence ID" value="XM_037300226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMY5"/>
    <dbReference type="GeneID" id="59337760"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052374">
        <property type="entry name" value="SERAC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182:SF2">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="21354" checksum="4FE67BCF4EF783D5" modified="2022-01-19" version="1">MPSWGLKLLHPSPAESTLDSFYVDIVAVHGLGGHQTGTWTDPGTGVIWLRVLLPFTILTARVWSFGYEANASTFFGDGSADRIQQHAHTLIAELEAVRALSSAAERPIIFVCHGLGEILVKKALAHSATQTSKNVDHRYSIYISTYGIIFMGTPHSGIEHATWQTIAQEARTTGGAPREMLKALTKNSETLQNITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN23</accession>
    <name>A0A8H6CN23_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009286</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="402"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="439"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="472"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="65858" checksum="6F7C5854B0774753" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQH6</accession>
    <name>A0A8H6CQH6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008915</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155779.1">
        <property type="nucleotide sequence ID" value="XM_037299778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQH6"/>
    <dbReference type="GeneID" id="59337310"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05911">
        <property type="entry name" value="Firefly_Luc_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF149">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="22"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="452"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6227471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="554" mass="61444" checksum="96D59CEDA05EE4D1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQI7</accession>
    <name>A0A8H6CQI7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007743</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155949.1">
        <property type="nucleotide sequence ID" value="XM_037298613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQI7"/>
    <dbReference type="GeneID" id="59336140"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="357"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="472" mass="53023" checksum="8972408E13F6A234" modified="2022-01-19" version="1">MNQFQESSLLHQAFSSLPVQSQQHLALIPQGQQQVSISDMSNASQTTSVISALGTIIGYIGSEAATEDVFERLLWPQRFFNAFSWQDLLEIGLLHPMGGPMHKAALNTLDKFHRAGLFRGRNLGNMLGTAFFHDTGLKYKMHDPPPGSKGKEYVRNGLWVQAVARISLVVQCQKEQNPESGVKPPRLVRARSVVNFLELSYVDGTTDPRKTVRYDTGSTSCRTLLAIVWSEITGLVVGGFAVGYWRSYFSFLWFLPLALKLLSAIFTIQREALLTKPTEKEADGEETKRFEVNTHGHGFLVIEGKESAVLQFFRHYGHPIRNRAREIAQISIVVAFGLAFPIGLMCSLIWMPVGLQYVWLGYQLYATLAMYIHRFTKAHQWARTEARLAQKFATGNNEERVAYLQDERGNTVMGKLTRINVGNYGEGQQVLKDFLHGKPKEIRKGKSDHDLHESESSDTLKSTPEDSDDSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQR4</accession>
    <name>A0A8H6CQR4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_007610</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat NOL10/ENP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155816.1">
        <property type="nucleotide sequence ID" value="XM_037298480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQR4"/>
    <dbReference type="GeneID" id="59336007"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056551">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040382">
        <property type="entry name" value="NOL10/Enp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056550">
        <property type="entry name" value="NOL10_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23098">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23097">
        <property type="entry name" value="NOL10_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Nucleolar protein 10-like N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleolar protein 10-like second" evidence="11">
        <location>
            <begin position="413"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="10">
        <location>
            <begin position="549"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="506"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="567"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="625"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="653"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="678"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6227882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="942" mass="105219" checksum="5BA91A07DBC8876F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRC2</accession>
    <name>A0A8H6CRC2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155840.1">
        <property type="nucleotide sequence ID" value="XM_037298504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRC2"/>
    <dbReference type="GeneID" id="59336031"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="118" mass="13175" checksum="D6424422781A7C81" modified="2022-01-19" version="1">MGYHVDSAFLPVLFSFGDEPHLAESGAINVATESLGAYALDAKNQYTVTETNKGMEIVTRKTNFLTEKTAEFAEKSTMDNTVIKWITIVSLFYLPGSFVTVMEWSFFWSSRLNEDGIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS28</accession>
    <name>A0A8H6CS28_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008237</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156441.1">
        <property type="nucleotide sequence ID" value="XM_037299105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS28"/>
    <dbReference type="GeneID" id="59336634"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0915">Sodium</keyword>
    <keyword evidence="5" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="368" mass="38966" checksum="1D671F1EA09112EB" modified="2022-01-19" version="1">MAAASYAGTSNLFAVYNAGDVEKSERIEVTQSILRGSVQQAAHNQSSVTTNESRNAPDDRTDRGEQLDVPGIAITQAIQEPDGRNRKTVQAQGSLSGVSIYGKFIEQPVARVLKPSFFASANNFGSVWGLKADCMCQASIGFSIPATRMFTGAVIWRGIIFTILMLIGKLVTGIWLIRIAAPSGTMIAKIKAFLSHLNGPQWSCLGLAKSAKCSKSEARNTANDPGSKPMPDVRIALATDSGQGPRQSSDRPSQSASIPQLGSVIPISPKATISTTKPRSLYPGSIVGTAMMARGEIGFLIAALAESTGIFASHDDSAAREDSGSTIYLVVIWAVVLCTIIGPVIGRLISQKSHEAATATATARAWWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7I6</accession>
    <name>A0A8H6F7I6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006263</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147286.1">
        <property type="nucleotide sequence ID" value="XM_037297161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7I6"/>
    <dbReference type="GeneID" id="59334664"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056125">
        <property type="entry name" value="DUF7708"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24809">
        <property type="entry name" value="DUF7708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="857"/>
            <end position="999"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1045" mass="118385" checksum="8069F2A20594A258" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7T0</accession>
    <name>A0A8H6F7T0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147857.1">
        <property type="nucleotide sequence ID" value="XM_037296676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7T0"/>
    <dbReference type="GeneID" id="59334176"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="254" mass="26809" checksum="F27FACB2AF7DD977" modified="2022-01-19" version="1">MVASSWASGVESLLHCAHKSPKNGPHPRVWSLSGRKNHVVPNDRDHQSGHGFQSDPDGPSDQGVDEGAPVDGGKMTCEGEVIDVVEGAGKEDVVEGAGKEDVVEGAGREDVVEGTGREDVVEGAGKEDVVEGTGKEDVEMTDVSDEKADLADDEDNHERGVLRLVGGGTEIDEEVPELPHNGYGCVYECGHHNEYDGDTRKSAVGKPRLGPSCPGCPSAGLLSGRPWLGCSAESEAYAAVSTKREMIEWNLQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7U6</accession>
    <name>A0A8H6F7U6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147631.1">
        <property type="nucleotide sequence ID" value="XM_037297057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7U6"/>
    <dbReference type="GeneID" id="59334559"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034347235" evidence="2">
        <location>
            <begin position="20"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="254" mass="27765" checksum="8E31E09C621AD88C" modified="2022-01-19" version="1">MRISTLFTAASLFLIGSYSLPRPENLEPSRTIEDREAHVAPGAPFASHIGGAEWWKDRKSGGETPASNGTKLKEREAHHERPISEPSGLPEFFEHRVGGSGTPSTNETKIKEREAHHERPISEPSGLPESFKHRVGGSGTPSTNDTKIKEREAHHERPTSEPSGLPEFFEHRVGGSNTPSTNGTKIKEREAPPDGMTWMEFMKHKKHKGGSPSHGAFVPLSTGTPSKELSRLSTSGTETGGTNSTKVRMMKFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F862</accession>
    <name>A0A8H6F862_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fungal N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147733.1">
        <property type="nucleotide sequence ID" value="XM_037296552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F862"/>
    <dbReference type="GeneID" id="59334049"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="567"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="64423" checksum="D160EEA133ADBD7B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8C8</accession>
    <name>A0A8H6F8C8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148472.1">
        <property type="nucleotide sequence ID" value="XM_037296490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8C8"/>
    <dbReference type="GeneID" id="59333987"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="138" mass="15501" checksum="4B5EDBE168B0FFCC" modified="2022-01-19" version="1">MGQNPPLPPPYLEDNLKLAAELKVPRRPSGHVSMKSHHCGKCHGVFKQTCIDAKHMVLCLKHKTYSKWEGQCPPCEREWKAQEKEIKEAEAALKADKEQEEAQATKISKKTKKTGKDGKKLGSKCVPGWVEAKKGDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8K5</accession>
    <name>A0A8H6F8K5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148064.1">
        <property type="nucleotide sequence ID" value="XM_037296883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8K5"/>
    <dbReference type="GeneID" id="59334385"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47431:SF1">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47431">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="2">
        <location>
            <begin position="145"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="503" mass="55951" checksum="EC4F7C314B8B53B1" modified="2022-01-19" version="1">MVEGLNVRLSPLRSPAPEYMGDGAGIQPKFATDFAPAMDLGADVEMPNPRFEDLAVISFQPQVYKSSSLEERPSIDTVYTSNFFSSFWPSHPFLPSSRHIEDYLRGSEGTSLIHAINYIGSLYACAGNSQLQTMTFPQKLHEYPQNIFAVQSLILLAISSHMFDNPTQAQAFLHTAIEIALAIRLHRQDFDTRLGKGSCAMAESWRRTWWELYILDIMFAGLNQTSNMWLKDAHPGARSLAEFNCRFFAEEEIEYSSFSYRIAAARALSGIISVSIAPSLQAPPSYTEAELVLENLVLHLPKHMQSFVNIDGSINEILFQTHMMIHAPRSILAASGLIHQITCAPNAATWHSESILHHTKKTVDAADGICQMISAPPSLFSRTPFFVCAVALQAIVHLSAYGIAGWSQKRPLMQQQIQMSIGALKKLSEIWEVASIVLSQIKCVARAVLDPPRNVRSPDDRRDTTSAHHSLKEAGIAETLPANFEDLVQGNDESWFNDFLAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F938</accession>
    <name>A0A8H6F938_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149282.1">
        <property type="nucleotide sequence ID" value="XM_037294594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F938"/>
    <dbReference type="GeneID" id="59332083"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050983">
        <property type="entry name" value="GST_Omega/HSP26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968:SF13">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE OMEGA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="41"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="216" mass="25143" checksum="1AED3AA7BEC38437" modified="2022-01-19" version="1">MPPPPDADLYEYGEATGPAKTLVNKHNEPQPVKLYAGWFCPFGSFHYTPWREVRKMSLVQRFWTVLEEKRIPSQYIEVNPYHKPESLLELNARGLVPPLQYDNKPLYESSVILEFLEDTYPEHGPKLLPADPYSRARARIWIDFVTTRGLELPNEGEGGVDERSWNRWKKWLAAIEEKRIIKETTSENQYYLLIYQRYADNTAQSEKATRAGKGVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9G7</accession>
    <name>A0A8H6F9G7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Translation machinery-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003354</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 4">Interacts with the 40S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The SUI1 domain may be involved in RNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DENR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149657.1">
        <property type="nucleotide sequence ID" value="XM_037294276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9G7"/>
    <dbReference type="GeneID" id="59331765"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11607">
        <property type="entry name" value="DENR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046447">
        <property type="entry name" value="DENR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005873">
        <property type="entry name" value="DENR_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048517">
        <property type="entry name" value="DENR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01159">
        <property type="entry name" value="DRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21023">
        <property type="entry name" value="DENR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="104"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="194" mass="21126" checksum="FDAF0D5ABCC19092" modified="2022-01-19" version="1">MAEAAPTDAAPTEPQARHIVYCGVCSLPPEYCEFGGTAKKCKEWLEQNHPDMHEKLYSEEAITASLSTLSVDAQKRAQKDSKKKEAKAAAAEAREAEAKKEAMVYIKRVERNKRKYVTAVSGLEAHGMDLKKVAKEFGKKFATGSSVTKTASGGEEIVVQGDNSDDIFDWVVDVGGVPDENVECIEDKKKKSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9H7</accession>
    <name>A0A8H6F9H7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003384</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149687.1">
        <property type="nucleotide sequence ID" value="XM_037294306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9H7"/>
    <dbReference type="GeneID" id="59331795"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09391">
        <property type="entry name" value="LIM1_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09392">
        <property type="entry name" value="LIM2_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04397">
        <property type="entry name" value="RhoGAP_fLRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000033">
        <property type="entry name" value="Rho GTPase activator (Lrg11)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000112">
        <property type="entry name" value="Rho GTPase activator (Lrg11)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000058">
        <property type="entry name" value="Rho GTPase activator Lrg11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF10">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="81"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="145"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="453"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="9">
        <location>
            <begin position="826"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="551"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1063"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="599"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1088"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1131"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1143"/>
            <end position="1153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1197" mass="133666" checksum="A440C12BAA4501A2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA77</accession>
    <name>A0A8H6FA77_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002845</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">COPI-coated vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF375">
        <property type="entry name" value="LD29171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="804"/>
            <end position="994"/>
        </location>
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            <end position="479"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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            <end position="1784"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2006"/>
            <end position="2037"/>
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        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
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            <end position="152"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
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            <end position="384"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
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            <end position="1773"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2013"/>
            <end position="2025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
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            <dbReference type="EMBL" id="KAF6220413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2037" mass="228498" checksum="2AFA2FEFDFBB2690" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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    </gene>
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        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
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            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="278"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
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            <begin position="15"/>
            <end position="24"/>
        </location>
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            <end position="41"/>
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            <end position="130"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="278" mass="31176" checksum="1720483501F0E0C6" modified="2022-01-19" version="1">MTKNYPSSSETLRNPHPHRSRAHRFGSESDETSNTADSNAHVTRDDVTHNYPFLRNPRRSHAQRSRSDDNRNASNRNPRLPRGEASDGSSEEGLGEFVTSRDRESDSDATSSDGAHASASSRHSNRGGSSRRADEATSVDSRARQARRDVLMSRLQRREDAGRQARYAEMDRNDRLHHETDQDCRAFRLSLNRDRREDAHGSSASSDTNSDEPMARREYQGRGIGARRDDYDDRDPGPSTPRGYATRAISARREEYEDDSDGPSAPQGYGGRSGVRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAC0</accession>
    <name>A0A8H6FAC0_9LECA</name>
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        <recommendedName>
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        </recommendedName>
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    <reference evidence="9 10" key="1">
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the small GTPase superfamily. Arf family.</text>
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    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Arf6"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ADP-ribosylation factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041838">
        <property type="entry name" value="Arf6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
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        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="3 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20942" checksum="539F1B80A7C38A60" modified="2022-01-19" version="1">MGGSVSKIMGKIFGSKEMRLLMLGLDAAGKTTILYKLKLNSDVTTIPTVGFNVETVTYKNVKFNVWDVGGQDKIRPLWRHYFSGTQGLIFVIDSNDRSRIDEAGKELHKIILDREMKEALLLVFANKQDIPGAMTPQEVQERLKLSRLKDRIWYVVPSCATTGEGLFEGLGWLSNNVKTPPQRQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAP6</accession>
    <name>A0A8H6FAP6_9LECA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">HO133_002383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="59330796"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="184" mass="20854" checksum="7919C42A9BAF50D9" modified="2022-01-19" version="1">MAGPTDLKRLWRRYREYRVTKKQKSSQASSESSSRDSKESTALEEATIMVVGLGLDTKPEEEHIEYARDDDHRFINNPHGIGCSRTYEVPYDLATNSLLSTSPGSMRPRVPRGVNVDKSDALFSFPELGAWKTESIDTVKGKRKGRMPGDSADDRYKFLLDGPATRAKFSAKSFTMVRQRAWTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAS5</accession>
    <name>A0A8H6FAS5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">M protein repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150676.1">
        <property type="nucleotide sequence ID" value="XM_037293023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAS5"/>
    <dbReference type="GeneID" id="59330510"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23313:SF0">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23313">
        <property type="entry name" value="TSEC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
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        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="282"/>
        </location>
    </feature>
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        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="67266" checksum="BD7A39E1D4E022CF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAW5</accession>
    <name>A0A8H6FAW5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002725</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150479.1">
        <property type="nucleotide sequence ID" value="XM_037293650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAW5"/>
    <dbReference type="GeneID" id="59331137"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031213">
        <property type="term" value="C:RSF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028938">
        <property type="entry name" value="Rsf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14296:SF3">
        <property type="entry name" value="DIKAR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14296">
        <property type="entry name" value="REMODELING AND SPACING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="434"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="182"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="295"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="495"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="295"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="355"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="580"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="610"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="647"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="692"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="722"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="754"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="781"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="832"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="851" mass="95557" checksum="2B821818B4868537" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB28</accession>
    <name>A0A8H6FB28_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151352.1">
        <property type="nucleotide sequence ID" value="XM_037292813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB28"/>
    <dbReference type="GeneID" id="59330299"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="42827" checksum="AFC2E809B822FFFB" modified="2022-01-19" version="1">MTPGEEHLGGRFWLMSQLYAYFNPYTPIYPVNEELLVRRRGWLTLKIVRSDPESFSLLRIPPPVFAQPSAYPRTIIIYTIRTSHSVFAFVSETKTKFLLWLCEICDPHSQSKNASGNRTLYIKGTKTSLPLINPSPKAPASVTKSMTTMNTSASYLQVPARGRGLALGFRIVILVLDFVTLVALSISLGLYHKWIPSTSYPTTALKPSKHTDWTDPIVLAAVLVSFMWTSFITIRPAWTSKALHLGYYVAFEVICLVWLLACTIPALTLRESGFKDLVAISNTCDMGSVTLMNGDKVPWVCMPHLNTLKKLQVATYSVACAVAVLHFTLFALACHSKTHLPQASAPSHSIDNTPNQQTIAHETQDPTTRGSESDSTSPPRTVAVAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBC4</accession>
    <name>A0A8H6FBC4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD dependent epimerase/dehydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150974.1">
        <property type="nucleotide sequence ID" value="XM_037293321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBC4"/>
    <dbReference type="GeneID" id="59330808"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040632">
        <property type="entry name" value="Sulfotransfer_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36978:SF4">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36978">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOTIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17784">
        <property type="entry name" value="Sulfotransfer_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27028" checksum="BFB752F7DC260FC8" modified="2022-01-19" version="1">MTIALQDLGYGEVYHMRSAYASERDVELWIQALEAKYEGKGSPLGREQWDSILGHCEATTDIPGAFFATELVEAYPEAKVILTTRTPESWHASMMQTTWAVYQDRVRWIAAKLDGPTSRRRRMSDLFFQHFFGHNFPRTGKEVFIEHNVAVRRLVPAEHFLEHRATDGWEPLCKFLGKAVPQDKAYPNTNDVKSFRSRFQAMNKGIFIQVLTSLAPKAVPIVAITSILCWMFWASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBK2</accession>
    <name>A0A8H6FBK2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001451</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151800.1">
        <property type="nucleotide sequence ID" value="XM_037292381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBK2"/>
    <dbReference type="GeneID" id="59329867"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="63" mass="7064" checksum="004D94A4877E88DB" modified="2022-01-19" version="1">MCEYTRNYYVYSNCTDPAMHFTRTSTEGNAKKKCAKAPHERFIVVSGSQSPPFRKEGAGDVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCB0</accession>
    <name>A0A8H6FCB0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000049</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152424.1">
        <property type="nucleotide sequence ID" value="XM_037290989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCB0"/>
    <dbReference type="GeneID" id="59328468"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19496">
        <property type="entry name" value="Elp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019519">
        <property type="entry name" value="Elp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10483">
        <property type="entry name" value="Elong_Iki1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="330" mass="36434" checksum="62BD3494058E2E29" modified="2022-01-19" version="1">MSDRALQHRRVHNLLLIQKLLASRDASSPFTLVLDSVEQSAGPLVRHYIHHAKASKMDVVFVSYESLKPPADVTALIKARGKDPSAVRKEIERCVSSSRRTLLILDSLYPLTTHPSLPLSSFLSSLPSPTTSLLAIYHTDVPLPSPSLSLTTTTTSSIYTPSPLTLLRYLATTVLTTHSFSQMLARKRAKEKSRPDPLFGTDEGKEGVLVGVGANDKRACVVEMEHRRKSGRGVEEWFFLPEVNGSGGVVLLEDQELYRRSDDREGRSDGVEARVELATFELGLTAKQRRDREGVVLPYFDAQKGAGGGGGRILYDMGVEDDFDEEEDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCG5</accession>
    <name>A0A8H6FCG5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152115.1">
        <property type="nucleotide sequence ID" value="XM_037291753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCG5"/>
    <dbReference type="GeneID" id="59329235"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="257" mass="29320" checksum="56F89F5DF0C015C6" modified="2022-01-19" version="1">MESVLIAGDIYIRPAKRNRHAQQFWKSLSSRPKRFVDAMAILFRHLSGQSQRLVTITTDAHLKGQSTIQQPGIQLLATCKQAYNDGHYMFFAMNTFHWPPGPVNTKGWYVNLRPEHQKMIKTICIDFHLADLTYGDMNAVEVSARRGGGGRPSNYDANAWSSEAMLELAVRMWNIILCSYSRSTPQNLRRFVGLERVIFQSSIGRCVLNSQLGTDENELVSLELFVAAVSHHFGTILQLHVTKRGWKNTKKWLERLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD42</accession>
    <name>A0A8H6FD42_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS10m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">37S ribosomal protein S10, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Mitochondrial ribosomal small subunit protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000302</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152676.1">
        <property type="nucleotide sequence ID" value="XM_037291241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD42"/>
    <dbReference type="GeneID" id="59328721"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000003">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="8">
        <location>
            <begin position="77"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00078476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="254" mass="28845" checksum="D6729A30390B488A" modified="2022-01-19" version="1">MPAPFQTPPSKPANASTGLLGEGYNTDAQMAQTTPGGPPQEYIPPEQRDDPRLPRSVQAIYLKPLKRRAQYGVPTCDLQLRSYNVRNVEFFADFALRAAYYLHLPAAGPIPLPRITERWTVPRSNFIFKKSQENFERITLRRLIQIQDGHSETVEIWLAFLRKHAYYGVGMKANVWQHEKLGRCCALSPMVPMLKSILGVGKTMDVSLENLKQAMEPKWAHFGRRKTLETSERAFEMMKSEAFQRTGIPLPSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD78</accession>
    <name>A0A8H6FD78_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="10">2.7.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000407</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152781.1">
        <property type="nucleotide sequence ID" value="XM_037291346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD78"/>
    <dbReference type="GeneID" id="59328826"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09135">
        <property type="entry name" value="PLDc_PGS1_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016270">
        <property type="entry name" value="PGS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000850">
        <property type="entry name" value="Phospholipase_D_PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="432"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="432"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6223564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="539" mass="59825" checksum="19077378AA4C0100" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDC7</accession>
    <name>A0A8H6FDC7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inner centromere protein ARK-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010909</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INCENP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153392.1">
        <property type="nucleotide sequence ID" value="XM_037301758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDC7"/>
    <dbReference type="GeneID" id="59339299"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005635">
        <property type="entry name" value="Inner_centromere_prot_ARK-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13142">
        <property type="entry name" value="INNER CENTROMERE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13142:SF1">
        <property type="entry name" value="INNER CENTROMERE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03941">
        <property type="entry name" value="INCENP_ARK-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inner centromere protein ARK-binding" evidence="9">
        <location>
            <begin position="1153"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="167"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="440"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="612"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="355"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="458"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="511"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="548"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="613"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="649"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="746"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="789"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="818"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="848"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1075"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1153"/>
            <end position="1166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1260" mass="139150" checksum="6C4C8187844D7254" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDW5</accession>
    <name>A0A8H6FDW5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IgE-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010843</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153328.1">
        <property type="nucleotide sequence ID" value="XM_037301694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDW5"/>
    <dbReference type="GeneID" id="59339233"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052820">
        <property type="entry name" value="PhiA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42047">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42047:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="21397" checksum="E637290EDA569F16" modified="2022-01-19" version="1">MHFTKTLLASGIPLLAFCNHLKLKPLTISPPFHLLAIHSTSPIHLQPINARLGGFYIGERPETYCPLAPCPIVNQTGFIVANGKSFLDVSVAGGQNLYVDPHGALSFTRAGSPGAIPEGAATANFTYTPAIDDDDGPGSFMFSGPGADGFLACPSDGPTGIPYSVFANVTGLTDAIVPGGDVSACVGIAAAAFAYTYEDNVVAYGYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE14</accession>
    <name>A0A8H6FE14_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153478.1">
        <property type="nucleotide sequence ID" value="XM_037301844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE14"/>
    <dbReference type="GeneID" id="59339385"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47877">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT DOMAIN-CONTAINING PROTEIN / LEA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47877:SF3">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT DOMAIN-CONTAINING PROTEIN _ LEA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17316">
        <property type="entry name" value="Perilipin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="272" mass="29549" checksum="17E64A24F4F6D267" modified="2022-01-19" version="1">MSSQTMANGDNSSQTIDHLTSYPVVSDAISTFKSNPYGKKSLDLSNTGYEKLVAPFVPYAKRPYGYVAPYVAKADSLGSDALSRVDHTFPIVKENTEKIKGTVLDYAYFPFRLVGDSKNYVLDTYSSEYKKCGGDGYVAGSKAMITTGLVVTSDTLSWLSTFLGQKKEEGTDYAVKKYGTASNYAHDKSGQAMQYAEAKSEQAKNLAYQKKEQGKDMAANAKDMAFQKTDEGKDMAANAKDTTQAKGNEAKDTAQSKGKEAKDTAKEKSGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEH3</accession>
    <name>A0A8H6FEH3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010292</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153963.1">
        <property type="nucleotide sequence ID" value="XM_037301146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEH3"/>
    <dbReference type="GeneID" id="59338683"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L7/L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_bL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_bL12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_bL12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_bL12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL12M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="Ribosomal protein L7/12, oligomerisation (N-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL12 oligomerization" evidence="6">
        <location>
            <begin position="57"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein bL12 C-terminal" evidence="5">
        <location>
            <begin position="120"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="15"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="187" mass="19843" checksum="F0E14A04F1BA3590" modified="2022-01-19" version="1">MSLSSTSTARCCARALRNSSRPRPPCPASSVASQRQRRVPSRRWESTAAAASTNPKVSSIVDQISQLTLLETADLVSNLKSRLNIPDMPMGGFSAAAAPAAAAPVEEEEAAPAAQEKTLFALTLKSFDAAAKPKVIKEIKSMLGLSLVDSKKFVESAPKQMKEGVPKDESEKIIETMKGLGAVVTMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFP2</accession>
    <name>A0A8H6FFP2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009009</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154696.1">
        <property type="nucleotide sequence ID" value="XM_037299870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFP2"/>
    <dbReference type="GeneID" id="59337404"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF158">
        <property type="entry name" value="MEMBRANE PROTEIN PTH11-LIKE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="63"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="382" mass="41411" checksum="5E55DE29044C1DE0" modified="2022-01-19" version="1">MSIPAGVPLSEIPSLQPPPGVTPNFINPPTIAPALIAVNGVFLALMLVAAGTIVSGPTVQAGSIAHTGVIIEQTKHGYGKHLWDVPLSVLTKSDVHVLLAENVTGQPTTFLIKLSIMLLYFRIFAVNQTMRYWILGGIGLQVVVYTATTACAIALESVCTAESAGTNSFCVNQYKNTVFQAVFSFTTDLYVLVLPIKSVLNLQLSPRRRIGVMILFGTGLIACVASLVRLISTIQQEHLRDSTYQGAYISIFTAVEMNTGIIAGSLTTLPTFLKKSGIASFNLSTLESLRTQLFTRSKDPLDDGSTKSNTFSKNPFKKSDMHLHGDNYIKLGKIKVTSLDKPQHNASQNDVETDHSNEGIFRTDEFDVAYSGNGRMKENNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG65</accession>
    <name>A0A8H6FG65_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155640.1">
        <property type="nucleotide sequence ID" value="XM_037299639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG65"/>
    <dbReference type="GeneID" id="59337170"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="255" mass="29703" checksum="C371F7880394D164" modified="2022-01-19" version="1">MSLNNVEGDDEEELTKIDTLCDILSSFDEGRTPSEYKRDVVDNQQPSMSFDDIFVPGDISRTIYRLAIYDEAFYQYLRQVVPPDVCARQYYNKQLPEQWRDMDVPKCARTLRLLVHQLCEDRERRTTNAPLGIGAVSLLAETLTDLVEEVCFHNRDIYEGSSWNRNQEPNEPERERNLYAYLIGNPPFNPSLLLWMRDHLIIARLRAFPADVGRSLSERLTTIKENIDEWDADNMPASRAYAGRIEDMLHEYDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH48</accession>
    <name>A0A8H6FH48_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">CCR4-NOT transcription complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_007659</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155865.1">
        <property type="nucleotide sequence ID" value="XM_037298529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH48"/>
    <dbReference type="GeneID" id="59336056"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051254">
        <property type="term" value="P:positive regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16618">
        <property type="entry name" value="mRING-HC-C4C4_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12438">
        <property type="entry name" value="RRM_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000257">
        <property type="entry name" value="CCR4-NOT core complex subunit Not4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000006">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034261">
        <property type="entry name" value="CNOT4_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039515">
        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="18"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="120"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="247"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="450"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="522"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="635"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="747"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="306"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="542"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="755"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6227931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="825" mass="89353" checksum="063FE8D9AEEAAF0B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHU6</accession>
    <name>A0A8H6FHU6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006986</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156517.1">
        <property type="nucleotide sequence ID" value="XM_037297882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHU6"/>
    <dbReference type="GeneID" id="59335386"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF16">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="3">
        <location>
            <begin position="237"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="612"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="774" mass="88265" checksum="D4FB44896EB390C9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIP0</accession>
    <name>A0A8H6FIP0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Terpene synthase</fullName>
            <ecNumber evidence="4">4.2.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007372</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the terpene synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.-" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156898.1">
        <property type="nucleotide sequence ID" value="XM_037298263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIP0"/>
    <dbReference type="GeneID" id="59335771"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034686">
        <property type="entry name" value="Terpene_cyclase-like_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35201:SF4">
        <property type="entry name" value="BETA-PINACENE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35201">
        <property type="entry name" value="TERPENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19086">
        <property type="entry name" value="Terpene_syn_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01020">
        <property type="entry name" value="Terpene_Cyclase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6229256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="361" mass="41324" checksum="89A753FD09DF7007" modified="2022-01-19" version="1">MGSTETMIAHESHSKDQAPAREMMLRNSEASCLGGSTPQKLIHEVVVKYKASVPREEPHQANASSHEEEVPSPADKAYGENGTVRQQMVYLPDMPDEFRNVCDWLNWAFDFDDPFDGGELRDRPEEAKKLVDNLLVTLEATSLEGVVVSENILVEVFRSVWLRLARDSSKETQGRFRGSMTDSCNGLIQQTEACIDPNNFDLNGYLATRCQTIAAYPLYAMVEYCYGIAISDEVIACKSIQTIQRLSTELTVLQNDVLSYHKEKRLGFEHTLVSIYIRQGKTQTEAYDCVDQLMKDRYRDWYLALAELPMWGEDVGKQVQVYIQGCQNMVVANLNWSFKTERYFGREHWNVRRTRIVPFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIU9</accession>
    <name>A0A8H6FIU9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007456</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156982.1">
        <property type="nucleotide sequence ID" value="XM_037298347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIU9"/>
    <dbReference type="GeneID" id="59335855"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF285">
        <property type="entry name" value="RETINOL DEHYDROGENASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="309"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="357" mass="39841" checksum="59106F0D5FCCB82F" modified="2022-01-19" version="1">MITGGTSGIGAAVVQHLATRGAQIILLTHHMPSDPFLVDYIDDLRASTNNELIYAEQVDLSSLHSIRVFATKWVDNAPPRRLDMIILSADSLIPRFGITSKTKDALDPVWGINYLANFHLLSILSPAIRAQPPDRDFRIVFATCSSYVGGDLKPLKDTRDPLPPGRKYETSKLACMIFAQAFQKHLEAYQRPDKQPNNARVIVVDPGFTRTPGMRRWLTMGSLWGLLFYLVTWPFWWLVLKSPVQGAQGFLRAAMEAELGRGSAGRLLRECKEVDYLRKEIRDENAQKRLWTFSEKQVEALEKDGAIKRSLAKKENEEMEKAKATAPGQNGKGVAAEKEKPANASKAPGSRRSRKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ36</accession>
    <name>A0A8H6FJ36_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157071.1">
        <property type="nucleotide sequence ID" value="XM_037298436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ36"/>
    <dbReference type="GeneID" id="59335944"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034156628" evidence="1">
        <location>
            <begin position="24"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="163" mass="17677" checksum="869DC628A1258C0B" modified="2022-01-19" version="1">MKSSILIGVAITGWMRALPSVAAQCSTIEDVRLTFYSRPDNGPPPGPDNTFDCGRGKGLDGKPIAGDPITFATATDNNNLPRCGIVYVPLLRKYFRNEDDCADCNLWTSLNQESRGNHQISCENNLPEGSQTIIKNPPGNLAVDSMLSPPAEYSLLPYADPLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJX5</accession>
    <name>A0A8H6FJX5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tyrosinase</fullName>
            <ecNumber evidence="3">1.14.18.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_004204</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2 L-dopa + O2 = 2 L-dopaquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:34287"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57504"/>
            <dbReference type="ChEBI" id="CHEBI:57924"/>
            <dbReference type="EC" id="1.14.18.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosine + O2 = L-dopaquinone + H2O</text>
            <dbReference type="Rhea" id="RHEA:18117"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57924"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="EC" id="1.14.18.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.18.1" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157124.1">
        <property type="nucleotide sequence ID" value="XM_037295123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJX5"/>
    <dbReference type="GeneID" id="59332613"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004503">
        <property type="term" value="F:tyrosinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042438">
        <property type="term" value="P:melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041640">
        <property type="entry name" value="Tyrosinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF76">
        <property type="entry name" value="SHKT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18132">
        <property type="entry name" value="Tyrosinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00498">
        <property type="entry name" value="TYROSINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-0470">Melanin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034167640" description="tyrosinase" evidence="11">
        <location>
            <begin position="21"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="12">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6229867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="542" mass="59363" checksum="1D52DD5B56679044" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJZ7</accession>
    <name>A0A8H6FJZ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable lysosomal cobalamin transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004230</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Probable lysosomal cobalamin transporter. Required to export cobalamin from lysosomes allowing its conversion to cofactors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family. LMBRD1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157150.1">
        <property type="nucleotide sequence ID" value="XM_037295149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJZ7"/>
    <dbReference type="GeneID" id="59332639"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072665">
        <property type="term" value="P:protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050854">
        <property type="entry name" value="LMBD1_LysCbl_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16130:SF2">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORT ESCORT PROTEIN LMBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16130">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="10" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="515"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="572"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6229893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="595" mass="66742" checksum="17CCA86D9D321D18" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK58</accession>
    <name>A0A8H6FK58_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157306.1">
        <property type="nucleotide sequence ID" value="XM_037295305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK58"/>
    <dbReference type="GeneID" id="59332795"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="259" mass="28442" checksum="B082B3B9B53A80CB" modified="2022-01-19" version="1">MELARALTSRKRNISDSSRFEPKRAASMRRTGTIKRSEISAPLQLLSTTNVLAFNAPNIYGSSDTESEGSMTFSNSTRATTPDNDPPSPVEPNHLSSYFADANRSVSRRSKDSSDCDSPAIPQRMPSHTKKSHQLAARKRSIQQTVPPPVEIHSTTAARNSIEMFSHKPEADHPFGAELTKVNELAEEMGARDVLILDEEEQYLMSNGFCKFGAEDYLDEIMGLFGCSYNNPFSKDCPAMHLRASTSVGTGFPMASQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK87</accession>
    <name>A0A8H6FK87_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157287.1">
        <property type="nucleotide sequence ID" value="XM_037295286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK87"/>
    <dbReference type="GeneID" id="59332776"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="97" mass="10003" checksum="2DEE5A3C268E8FC9" modified="2022-01-19" version="1">MGNELLLIIGFTIFSLVVTMAWVGGYLDTYQHTLQNVLLDKTGENRASYGLKTVLTGQKTGDQDLDAVQDGVGSGVGGLVGKGGVGESIGSTLSKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKL5</accession>
    <name>A0A8H6FKL5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004595</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157512.1">
        <property type="nucleotide sequence ID" value="XM_037295511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKL5"/>
    <dbReference type="GeneID" id="59333002"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15485">
        <property type="entry name" value="ZIP_Cat8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000007">
        <property type="entry name" value="C6 transcription factor FacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF12">
        <property type="entry name" value="REGULATORY PROTEIN CAT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="612"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="641"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="680"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="693"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="724"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6230255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="845" mass="92969" checksum="9A0E2E3670681BC9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKY7</accession>
    <name>A0A8H6FKY7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004798</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 7">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peroxin-14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157712.1">
        <property type="nucleotide sequence ID" value="XM_037295711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKY7"/>
    <dbReference type="GeneID" id="59333204"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF5">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0811">Translocation</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="9">
        <location>
            <begin position="44"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="220"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="222"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="254"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6230455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="365" mass="39221" checksum="DE582243CC5280ED" modified="2022-01-19" version="1">MSDSEGPKSKEIPSWQRQEPSSLPKQSDDSPSQEPAEREPPESRAPLIEKASKFLEDDSIRDAPIARKRLFLESKGLTETEMDDLLEIQHTPEAAVMEDYGIEREGISQSTPPTAPQIPSTSSVPQEPPPAAPSKDNPPIITYPEFLLHAQKPAPLITANRLLTSLYIASGAAATIYGTSKYIVEPMVESLTSARHSLFEGAGANIDALNEKLKSAVSKVPEIPHHSKEDDSDKDSLSSDGARFFNRSAGTQTSPHLSRSNSSTSSDPDETLSPAQDHTSALLGIHSKLSDLLPADGKPTNPLGDSISELRSYLEKLPQANSLHLGGKARKMGEIDAVANVKAEIRGVKGVLLSARNFPSGVAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FLM6</accession>
    <name>A0A8H6FLM6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157838.1">
        <property type="nucleotide sequence ID" value="XM_037290964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FLM6"/>
    <dbReference type="GeneID" id="59328443"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="359"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="720"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="758" mass="84212" checksum="2658DF0F89262E77" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYE8</accession>
    <name>A0A8H6KYE8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH APSES-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007237</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156765.1">
        <property type="nucleotide sequence ID" value="XM_037298130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYE8"/>
    <dbReference type="GeneID" id="59335637"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990862">
        <property type="term" value="C:nuclear membrane complex Bqt3-Bqt4"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048315">
        <property type="term" value="P:conidium formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070197">
        <property type="term" value="P:meiotic attachment of telomere to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044820">
        <property type="term" value="P:mitotic telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037548">
        <property type="entry name" value="Bqt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38044">
        <property type="entry name" value="BOUQUET FORMATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38044:SF1">
        <property type="entry name" value="BOUQUET FORMATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0183">Conidiation</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0749">Sporulation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="HTH APSES-type" evidence="5">
        <location>
            <begin position="73"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="180"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="340"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6229123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="414" mass="44455" checksum="ACF2BE84D7789A6C" modified="2022-01-19" version="1">MSNVQINGTSERVLPSKRNPLLAPEKTQPHSELIAKRRLGQTNLAVKPGQVGTSNATKPENLGPFDYAHLRAPLPRSLKGSEIFAPHQNQPTPEVYFLMRRSTDGYVSATGMFKAAFPWAKHAEENAERDYVKSLPSTAQDEVAGNVWVSELYAIELAQDYGIVPWIHALLDEAPISQGTDDPATKAISPPPKFTFTANDRTLLPPPNGTTARASTPKARGRPRGTSPAKNASPGKPSTKKPRASKAAKEADAASAREASASLQATLDHTAPDGTSTAEPESADGEKVKIRVESSVEIKGDTERGETTTVNIEMPTGFPELPLPERPEEMIETARAMVEEARKADGEGSSSKAKRKAEEMDEESDGSADNELQPAKKARLLGEELKKEKVRTRALLGVAATLVIGAIIPLVLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYK5</accession>
    <name>A0A8H6KYK5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156871.1">
        <property type="nucleotide sequence ID" value="XM_037298236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYK5"/>
    <dbReference type="GeneID" id="59335744"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="208" mass="22233" checksum="BE0B929702066565" modified="2022-01-19" version="1">MCHPIVREWQACPPSTPVPAPTPAPTPPPSLPEILQIITFITHTTNNASSTLTSAAFQGLSTVVSGEAVINGTLTMTQFVFLAMMKNLLNGTKSVAAPTSLPTNFGAINCNQEGWGLCLSCTPAGVNPKIRKGTTITITRLPEPPDISLSRCLFSFLCANPRPGTSAQTVEGVEDEIQVNQLTVDVDGRNPGTFSDTNYKDTARHHRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYL1</accession>
    <name>A0A8H6KYL1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156828.1">
        <property type="nucleotide sequence ID" value="XM_037298193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYL1"/>
    <dbReference type="GeneID" id="59335701"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="232" mass="25448" checksum="76C9A9C0B6071E1F" modified="2022-01-19" version="1">MANNFRGQRDWPPKFGNLVDPNNEPHSMSFTGDADFTRYSGFGGGVAFRQRFPDRTMRDLTESSRREVVTQYPQGKGWPGAVGMMQAPMTISNGRNDGRARRSGHGIDASTSTKGKRMPMPKNVEDAMPHNGNELASHRSQYKCVEQGVLSNHGDVHQDYDYRASAASSPSFYTSSTEEYKHEPPCTRHTRGVGCADRLENIGGRDVGPPCGTQPGVSGGMRRLPCLQAPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYN2</accession>
    <name>A0A8H6KYN2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156799.1">
        <property type="nucleotide sequence ID" value="XM_037298164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYN2"/>
    <dbReference type="GeneID" id="59335671"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="98" mass="10273" checksum="A52A2AFABBC39BF0" modified="2022-01-19" version="1">MYNNDGSRNPAEDSSRVGFSPIGIIMALSLAVTVTIALVALGLLRRYPGGKQAVPITSTHSGVISVASHRPPGDTEAHLLPVQWGAVISEDGLYCQTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYN5</accession>
    <name>A0A8H6KYN5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007332</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tryptophan dimethylallyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156858.1">
        <property type="nucleotide sequence ID" value="XM_037298223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYN5"/>
    <dbReference type="GeneID" id="59335731"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009820">
        <property type="term" value="P:alkaloid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017795">
        <property type="entry name" value="ABBA_NscD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40627">
        <property type="entry name" value="INDOLE PRENYLTRANSFERASE TDIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40627:SF4">
        <property type="entry name" value="PRENYLTRANSFERASE ASQH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11991">
        <property type="entry name" value="Trp_DMAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="200" mass="22694" checksum="207BED35301263A8" modified="2022-01-19" version="1">MSYLSTFEGQEDAPLAWLLSNDCVADTPGMRLKVYILSEADSLTKLNEMFNLGGRLKDAHITASFEGIRELWYHLFGLSGSDLTANDKVYVDKMKCVFVYEMRSTHGSEPDLDVKLHIPMWQLDKSDGQLSEVMASWFESHGHPDLAARYKSDLNKAFPKHGITENMGVGTHTWMSITHTPKTGLYMTMYLSPKLPEVYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZH6</accession>
    <name>A0A8H6KZH6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157222.1">
        <property type="nucleotide sequence ID" value="XM_037295221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZH6"/>
    <dbReference type="GeneID" id="59332711"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="182" mass="20241" checksum="421FAED99100CDDF" modified="2022-01-19" version="1">MPSIIQTAFFFEALVNVPAIVSLIFYPESTLRPALTTNASFSAAELNRTATFIARCAGVLILALTPQLLLALPDSKDCVGKRKQVYWTLGLGEFGLIPLFLWEAFRASDQDKLDWRGGLTKRAALLCAGMLIPPLAWRVFVFGWREHWFGPEGGRGRENGTSINQADADDLVRSWNLGRKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZH9</accession>
    <name>A0A8H6KZH9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157175.1">
        <property type="nucleotide sequence ID" value="XM_037295174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZH9"/>
    <dbReference type="GeneID" id="59332664"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="501" mass="57002" checksum="35AB5ED7C5C56F8C" modified="2022-01-19" version="1">MVAAKQLFRQNDYELAKSIKELKLSGYLPYRGVSSYSLTSDSLIQSGGFRAMRPETLKINLGWDHWDLNILHAMSVTNLKLLKLPVRLPSDCVRLGQALTVMPKLASLVITDIPDREEFLIELGYLGKGIMSCAPTLRELDIEMTNFNRPLSWAGDERFVEPQDDGFFFRKLFPCPPKEELLGLCERHSRHDTDPMTEAPLSLTKLRLKHLSLPWYSFGIIFNAKAIRQLHLPYSSADRQVWGFLETYAQLDSLTEISYDMLSAVFLGFLEQQSSLKELTFARPQDQCDGTDVIFYGTSAHMILRVSREAPRLGPDAGAEYPNLDNFLSSLEDMTMLKHLVLPADMYTITSGCLCSIATSLTSLEHLELGFDYNDLELQRIFASCFLCGTRTLKKLTFLPLKQPKPLPDFNPAQFHSLVVSLADEVSQNLKLVRYMPLDQIYGHKYCGDIDVYYHRETPVDGKWWMCLPPHESEHIFAEGRTPSIPVDDWDGGFEAEQDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZJ3</accession>
    <name>A0A8H6KZJ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peroxisomal membrane protein PEX13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Peroxin-13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_004332</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts (via SH3 domain) with PEX14 (via SH3-binding motif); forming the PEX13-PEX14 docking complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxin-13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157251.1">
        <property type="nucleotide sequence ID" value="XM_037295250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZJ3"/>
    <dbReference type="GeneID" id="59332740"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11771">
        <property type="entry name" value="SH3_Pex13p_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000128">
        <property type="entry name" value="Peroxisomal membrane protein (Pex13)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007223">
        <property type="entry name" value="Peroxin-13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035463">
        <property type="entry name" value="Pex13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04088">
        <property type="entry name" value="Peroxin-13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="318"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="393"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="408"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6229994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="452" mass="47958" checksum="2FC818141081B66D" modified="2022-01-19" version="1">MAAVSPPKPWERANAAATGASSTSTANPHTTTPITSSSSPPALPDKPSSLASVVNRNSSTYSPVNQNRYSSSSYSGVGGYSSPYSRFGGMGNSMYGGGYGSMYGGMGGMGGMGMGGYGGGMYGQPGMMGNGVSNDPNSLTNTFSQSTQATFQIIESLVGAFGGFAQMLESTYMATHSSFFAMVQVAEQFSTLRTTLGSILGIFTVLRWLRTLFAKVTGRPPPADATSLTPSAFASFQGLSPSGPSLLPNGQPTPSKKPFLIFLAAVIGLPYLMTKIIRSLARQNEEQQNLYNQEGRMILGPDGQPMQEQQPHQPIDPSKLDFCRLLYDYTPESMTQGGSPTEGIDLEVKKGDLVAVLSKSDPMGNPSDWWRCRARDGRVGYLPGLYLEGIQRKQQPQIDDGGRVNTMSSLAAAGSGEGSRSNSLKVEAKGPKVESKAADISAESFQRSQFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6B0</accession>
    <name>A0A8H6C6B0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147157.1">
        <property type="nucleotide sequence ID" value="XM_037297447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6B0"/>
    <dbReference type="GeneID" id="59334950"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="115" mass="13312" checksum="28CDC071E4C08AF7" modified="2022-01-19" version="1">MSRTKPHSTHCTHHPVLLRHQAVYLISLLIKHAQQIQFDITRHNINDWNRVTDEFNARFEGKPCTTGSGEAQMKKTVAMLYCALMENEEEYKRLFAEAEQAFRAEKRGAKKARVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6B7</accession>
    <name>A0A8H6C6B7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_006706</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147039.1">
        <property type="nucleotide sequence ID" value="XM_037297603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6B7"/>
    <dbReference type="GeneID" id="59335107"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23165">
        <property type="entry name" value="Prefoldin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000005">
        <property type="entry name" value="Prefoldin subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF74">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="397"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="428"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="495"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="540"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="582"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="607"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="640"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="675"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="271"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="718"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6217604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="747" mass="83464" checksum="206F59B110D402FB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6D3</accession>
    <name>A0A8H6C6D3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006736</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147069.1">
        <property type="nucleotide sequence ID" value="XM_037297633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6D3"/>
    <dbReference type="GeneID" id="59335137"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19146">
        <property type="entry name" value="AKR_AKR9A1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF7">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="33"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6217634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="384" mass="42552" checksum="C430A077CEB723A9" modified="2022-01-19" version="1">MAATLAHQPAPKPKSLLGYQRILAPTAGVRVSPLCLGAMNFGEAWSGFLGECNKETSFGMLDYFFENGGNFIDTANNYQGEETEKWLGEWMESRRNRDQIVLATKFTSPYPSEPVTIKSNYLGNSSKSLRVSLEASLKKLRTSYIDLLYIHWWDFTTSIPEIMKALHHVVASGKVLYLGISDTPAWIVSKANEYARQQGLTQFSVYQGLWNAAVRDFERDIIPMCESEGMALAPWGSLGRGAFMSPEDYEKKDEGRKVGQSENGKKVSAALDKLAKEKGTLITSIALAYVRHKSPYVFPIVGGRKIEHLKSNIEALGIELSDEEIDEIDGAAPFDHGFPLNFVFEYGGGPKYNSRMGPGDIFFVKSSGGLDTVPRSKAPKPHKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6L8</accession>
    <name>A0A8H6C6L8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin efflux carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006656</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146989.1">
        <property type="nucleotide sequence ID" value="XM_037297553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6L8"/>
    <dbReference type="GeneID" id="59335057"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794:SF2">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="158"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6217554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="55290" checksum="10BDCBF5D2A0F4B9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C730</accession>
    <name>A0A8H6C730_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147423.1">
        <property type="nucleotide sequence ID" value="XM_037297298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C730"/>
    <dbReference type="GeneID" id="59334801"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="463" mass="48778" checksum="8AA261A1E4F40A72" modified="2022-01-19" version="1">MSHNINITNLSGHDQDFEVHGWNSNQNMTVPAGQTFVISAADGSSGAIIALHFGFEGEQAEITKDGFGGKPRRDEYRLFILLNPRIGNDFIDMSNIMGAGGNMTVQQVNNPSTRKGDPIFMQNLNTAWQAADGPTKQSLQPCVQLDGNGNVVRIAAIKDYPQLETFVRSFADGQTYIGNGAWGGSSGNPSDNNQSSAAQGDTDIQVTYNDGDATPPTTAVSQFKKPGSKLVQTHQRDASPSGPGIILNNHTSSQQTYAFYNNYWNGNDTAGANFDHQDPTVQVDANGSLFVSLPTSFKGRVQRGTQLPATWVEFQIAASNDNAAHGDISLEQGCDGAATISATDGSGQSNGFTNDVLSGAPAAAIQDKPDGTPALATTVGNWMSGPNQAAIDWENQAVGQSKAYIQGGTGVPDVASTNQSAKGWDGLIFQLFEDALAPGVVSVTEAGQTITGGEVLERRGWGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7N1</accession>
    <name>A0A8H6C7N1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147611.1">
        <property type="nucleotide sequence ID" value="XM_037297037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7N1"/>
    <dbReference type="GeneID" id="59334539"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034487297" evidence="1">
        <location>
            <begin position="22"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="350" mass="38560" checksum="5A4C88A3F523EAE3" modified="2022-01-19" version="1">MFGIWLLLLFLQFYVFPLAQAASLPLPSQSDPITLTETTKLTRANHASISLTKPSNNISVFFGNPGDPIPAAELRHTLSVARASVQAYLPRYANEPISDSFFKTNISFPETGNSVCISVYAYGFGLSWLQLSQVLVILEGYMRGRGPGHPHTHYQQLEFYVQLRAGIEVAHGVVEFAPGARAVAKRTLVTTTLLQLPHANISSLGTVALPITFHIPRTNLDLNITALGLPIPQDTMLTTIESAYTEVILDHTDIDSPMPANPPFSFTATSGKRPHQFKTEILIIPYIGKQFSWALLCIVMYGLRDFLHQTSHFNSVSFQIIDASLGKMGSGDVLYWPVNEMASTKGLKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7V7</accession>
    <name>A0A8H6C7V7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005931</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148015.1">
        <property type="nucleotide sequence ID" value="XM_037296834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7V7"/>
    <dbReference type="GeneID" id="59334336"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045178">
        <property type="entry name" value="Fhl1/FHA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712:SF29">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="5">
        <location>
            <begin position="342"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="633"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="3">
        <location>
            <begin position="633"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="435"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="716"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="946"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="457"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="543"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="561"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="572"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="722"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="747"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="789"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="804"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="819"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="947"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="962"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="977"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1012"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1047" mass="112689" checksum="4B185729984EF76D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7W9</accession>
    <name>A0A8H6C7W9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carboxypeptidase</fullName>
            <ecNumber evidence="7">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005772</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147858.1">
        <property type="nucleotide sequence ID" value="XM_037296677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7W9"/>
    <dbReference type="GeneID" id="59334177"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000118">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF479">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034923139" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="19"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="542" mass="59723" checksum="2E9575E3248546B8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7Y8</accession>
    <name>A0A8H6C7Y8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147739.1">
        <property type="nucleotide sequence ID" value="XM_037296558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7Y8"/>
    <dbReference type="GeneID" id="59334055"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="58" mass="6399" checksum="F502A79FB5B6834D" modified="2022-01-19" version="1">MEEKVPTVGQLSSGLVGEDPEAGVETVDIERIEKVYRKLDHRIIPGMYPPEPPSPTPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C896</accession>
    <name>A0A8H6C896_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fungal N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147876.1">
        <property type="nucleotide sequence ID" value="XM_037296695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C896"/>
    <dbReference type="GeneID" id="59334195"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886:SF1">
        <property type="entry name" value="NACHT-NTPASE AND P-LOOP NTPASES N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886">
        <property type="entry name" value="SESA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="64916" checksum="5A893601D147E50E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8B2</accession>
    <name>A0A8H6C8B2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005954</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148038.1">
        <property type="nucleotide sequence ID" value="XM_037296857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8B2"/>
    <dbReference type="GeneID" id="59334359"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF236">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="331" mass="35545" checksum="DFD6CF9A4DE912AB" modified="2022-01-19" version="1">MPSAFSQAFPPAPTFTEKNVGSLKGKVIIVTGSASGVGYELAKLLYGQGGNIYIAARSSEKVDRAIESIKASVPSSKGHLAPLILDLADFPTIKNAVDGFLQKENRLDVLVHNAGLMTPPAGSKSKLGYDLEMATNCMGPFILNHLLESILTRTAHAESIPDHVRVVWVSSMIAAFVPPGGIQFDEKSGSPKVLANTMHNYMQSKVGNVFLASEAAKRLGGDGVISMSVNPGLMKTELQRHMPRVQSIIMGILFKPPLFGAYSELFAALSPQVKAEHNGGFVVPWGRFGDIPDHIEEGLKPRAEGVLNSKRLDAAGFYSVTRVNLVLVPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9R1</accession>
    <name>A0A8H6C9R1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148706.1">
        <property type="nucleotide sequence ID" value="XM_037296008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9R1"/>
    <dbReference type="GeneID" id="59333502"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="107" mass="12335" checksum="A67C93EC119E6198" modified="2022-01-19" version="1">MESKCGNQAFTVVNGDTESWQHMWPKLAKPFRCHTPRNQFTVDVGKDTDSVILLAEKPPMAERDTERDLEGNFTQGKMEQKIDSIKWSQREDVKKAMSKARKYGWTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAM2</accession>
    <name>A0A8H6CAM2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149422.1">
        <property type="nucleotide sequence ID" value="XM_037294734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAM2"/>
    <dbReference type="GeneID" id="59332223"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008402">
        <property type="entry name" value="APC_su15/mnd2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05841">
        <property type="entry name" value="Apc15p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="274" mass="29114" checksum="723447F552544CDF" modified="2022-01-19" version="1">MLSSLPTLHPPSPHTLWYASLSAGPPSQPHPTPTTTDPHQPHHAHAHRTNNNNNNPHQRTHLSILREHETAVRHRKANIRRFGAGWLRPPGVGKTLQGMADERAEREEGEVARAREYALAEAQAAAAAEAEAVEVVPGEEGEGERDLDEEVPDADADADVEGEGGWSDAEEDEEEGLEGAEQTLLEEGEGRGDGDGDLDEEVPEAEEGSYEHTDTEVEDESSDDGGLVRGGFGNGGRGLVLGANGGGVLGSSVFGSSPVRTGRRSSGRGRGREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAP1</accession>
    <name>A0A8H6CAP1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RTA1 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004155</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149121.1">
        <property type="nucleotide sequence ID" value="XM_037295074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAP1"/>
    <dbReference type="GeneID" id="59332564"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF9">
        <property type="entry name" value="SPHINGOID LONG-CHAIN BASE TRANSPORTER RSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="27764" checksum="585A2DF069B1C162" modified="2022-01-19" version="1">MSAEQAAYPNATLINNVNLSTVQTRPLAWATVDYIPSLAGNPFYLAVPYAVLVIQSILGIHYRSLVALFLGVLGEIVGYVAQVQMAQNPFLRTPFLMYIACLTLAPAFLTAGIYLCLSRITFGYGEHLARFAPRTYAIIFITSDILALVLQAVGGALADTAGTPKETQTGVNIMSRANPANLANDQSAFMVLGGGRMVVVVEVALTVADPGAVFEGAWEGKEMRLEGRCGGEGRRGEVEDWEMRVEDVGIGDRVVLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBP3</accession>
    <name>A0A8H6CBP3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003468</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149771.1">
        <property type="nucleotide sequence ID" value="XM_037294390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBP3"/>
    <dbReference type="GeneID" id="59331879"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013870">
        <property type="entry name" value="Ribosomal_mL54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L54, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08561">
        <property type="entry name" value="Ribosomal_L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="50"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="61"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="239" mass="25778" checksum="903EB1C0CA9BE799" modified="2022-01-19" version="1">MPAQKVLQPNLDIIFSPSPIYSLYTMICQRCARLLSPSFLRPATLRPLSTTSRSLRPESPPAATSTSAAQPFSTPFTPSPSKTPDIPSSPSTTPKAASKVPAHPPSSVAAGTPLRGLGYIKGQEGPVAKEDSEYPDWLWELLEAKKGDMTEEGVVGDAFAKSKKQRRLASKAARALKTGSHGTSHTVKVPLEEQSIDLPAGMGEAEGRKALEAREGLTSAMRTARRRKIKENNFLRGMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBS6</accession>
    <name>A0A8H6CBS6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149831.1">
        <property type="nucleotide sequence ID" value="XM_037293753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBS6"/>
    <dbReference type="GeneID" id="59331240"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="260" mass="30125" checksum="3500DAAB6EAE9785" modified="2022-01-19" version="1">MLEVAVSMKRDRRSGEGGHHQRGPDLPTRTQDWASFRQNIPYPSDSEESFARHHAERYGDEELLVDGQHTGSPHFPRRSERARIHAEYTTSNRQRDRTREAYGDTLKPATEEYESLSYNQREYYDSTSGGMLPRHHATLLPLARDREGPNYHNATNRERLLTMEYGYHSAEAQQRHWKSADKAYQEHEQVQADHARHRRFRSDPRDDDTVDCYSNSPVQSTEPHTRSRNQGLPPPGYYCDRLDTPPEEDDDGAGEAYGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC14</accession>
    <name>A0A8H6CC14_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thiamine pyrophosphokinase</fullName>
            <ecNumber evidence="7">2.7.6.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_002986</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>thiamine + ATP = thiamine diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11576"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18385"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the thiamine pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.2" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149988.1">
        <property type="nucleotide sequence ID" value="XM_037293910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC14"/>
    <dbReference type="GeneID" id="59331398"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00597"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016966">
        <property type="entry name" value="Thiamin_pyrophosphokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036371">
        <property type="entry name" value="TPK_B1-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01378">
        <property type="entry name" value="thi_PPkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031057">
        <property type="entry name" value="Thiamin_pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63862">
        <property type="entry name" value="Thiamin pyrophosphokinase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamin pyrophosphokinase thiamin-binding" evidence="8">
        <location>
            <begin position="198"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR031057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="277" mass="31269" checksum="39D4BEA02C0512B5" modified="2022-01-19" version="1">MVQDQNPKLWHPARFTHGHIHGSGDGPFGPDEVKQHTVIILNNPLENRNLLVDVCIEARCIVCADGGANRLHDLHLAGKEKTICFPNAICGDLDSLRLDVEEHYRSQDVKIIKDPDQYSTDLTKCLKYIRDQIVETEDEYESPRRGVDVAIFGSLGGRADQAFSQLHHLYTSSQDFPSMIGDLYLITAESVMFLLEKGKNRIHAPVGPRHFTENVGVIPIGRPSKVTMHGFEWDVTDWRTEFGTQMSTSNHIRAEIVEVETSERVLFTLETARKPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC43</accession>
    <name>A0A8H6CC43_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Isopropylmalate dehydrogenase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003142</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150144.1">
        <property type="nucleotide sequence ID" value="XM_037294066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC43"/>
    <dbReference type="GeneID" id="59331554"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF1">
        <property type="entry name" value="ISOCHORISMATASE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="4">
        <location>
            <begin position="9"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="601" mass="66008" checksum="54E9A3A051A0961B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCQ3</accession>
    <name>A0A8H6CCQ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sphingoid long-chain base transporter RSB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002014</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150595.1">
        <property type="nucleotide sequence ID" value="XM_037292942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCQ3"/>
    <dbReference type="GeneID" id="59330428"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF7">
        <property type="entry name" value="SPHINGOID LONG-CHAIN BASE TRANSPORTER RSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="34832" checksum="B3FE60A4C453E608" modified="2022-01-19" version="1">MANAGHNPNGLISYGPNENCTLTPGPQYCPASVGVYEYRPSLAANSVFIALFAIALVIHLGLGIKYRTWAFVFAMFWGCASELIGYGGRVLMWENPFSFPGFLIQIICITLGPAFYSAAIYLTLSKIIIFLGPQHARFSPKLYYWIFIPCDILSLILQAAGGALSSVSSGSSKIAVDVAIAGLSFQVFTLCVFISLCIEFTIRYIRAQRDQQRKTPLPTRFKVFVACLSLAIICILIRCAYRIDELSDGYDGPLIHNEPLFIGLEGVMIVLAVYSLVIAHPGPIFGRSDANTYETDVTATSDAFTEKAEPGMSTTQQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCW6</accession>
    <name>A0A8H6CCW6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL2m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002581</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150336.1">
        <property type="nucleotide sequence ID" value="XM_037293507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCW6"/>
    <dbReference type="GeneID" id="59330994"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000128">
        <property type="entry name" value="50S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_uL2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="10">
        <location>
            <begin position="102"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="9">
        <location>
            <begin position="208"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="85"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="366"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6220901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="380" mass="42583" checksum="D650B72D7C13DA6E" modified="2022-01-19" version="1">MLLLRLLRQPVQRCQSLHTFSRRLYPTEILPPPDPLIARNETLGVQSRPQYTPEERGVPILRTYKPRTPGLRHLKRPINDHLYKGRPYLPLTHPRRGQHIGGRNSHTGQITVRHRGGGHKRRIRTVDFQRLEPGKHLVERIEHDPGRSAHIALLTRTVGQTKSYSYIVAPQGMRAGEEVESFRQGIPERLIRELGGVHDAGMIAAKTAFRGNCMPLHMVPPGSIVYNVGLRKGGKAQLCRSAGTYAQVISKNEGDPKKALYVDVKLQSGEIRKIHRDCCATIGTASNVNFHRRQLGKAGRKRWLGIRPTVRGVAMNAADHPHGGGRGKSKGNVHPVSIWNQLTKSGYKTRTKSNVNRFVVLPRPRSQGKKAKAKKKKKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCZ2</accession>
    <name>A0A8H6CCZ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">inositol-3-phosphate synthase</fullName>
            <ecNumber evidence="6">5.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_002042</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10716"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="5.5.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.5.1.4" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150622.1">
        <property type="nucleotide sequence ID" value="XM_037292969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCZ2"/>
    <dbReference type="GeneID" id="59330456"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00787"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000069">
        <property type="entry name" value="Inositol-3-phosphate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000204">
        <property type="entry name" value="Inositol-3-phosphate synthase 1-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11510">
        <property type="entry name" value="MYO-INOSITOL-1 PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0398">Inositol biosynthesis</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="15">
        <location>
            <begin position="333"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6221187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="540" mass="58913" checksum="6CC528FA5EFE0368" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDL1</accession>
    <name>A0A8H6CDL1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150873.1">
        <property type="nucleotide sequence ID" value="XM_037293220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDL1"/>
    <dbReference type="GeneID" id="59330707"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="372" mass="40751" checksum="086908D944FB4480" modified="2022-01-19" version="1">MTSSSGVDAATEALIAQLMAEDLGESYYRHSTPIGASYHDYEEPLSSYERQCLDAENNPDEEGGEGSGWGPEDSDEVNGAVAPHEGLYPTGPTNEGTWDSTNENWDSRFVDEDGQVQEHTASGQSPQAVGANEDDNHSDSNSCSNPTNEELTGAPARVVSPAAYDVPNNPADETHNISAPMTLPTLSTPGVPDGHLDPSDHSSHREPFIPSFHLAPNEWLPASSALEVEGQWDDGLDCSSSKGKGKAVRAYDEFKRGLRDGEKESWDPNIADLEHRREEDADSDSEEEGEVRDEDLPFISIPWPCAENDELLSRREDAEVVEIRVGDDETLESILRDISLRDERRQKGKGVEGRIECADEDVSGRKDVAAWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDM9</accession>
    <name>A0A8H6CDM9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150754.1">
        <property type="nucleotide sequence ID" value="XM_037293101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDM9"/>
    <dbReference type="GeneID" id="59330588"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="28042" checksum="36E7FBCB68E27F0D" modified="2022-01-19" version="1">MSRLVAECGPLRPPAATYLVRGEIESRDTSAPIRINDSTNARPSSHDPEKERFRRHTRPSRSSSYQHSRVICADDGDDVDDNESKEHAFWILIYLSALSPLLALSIAPYTLLMGTLLLLLFPLCFCLKQIPVCAHCRHFLSPLLVFQLKLVYSSCDRDDTVHSETSGIMVLVLVSMLSPLYAMAIAVAAWVAGVFWFYTAMLGNPDGREDRDDGRETVLAVRGWWERWLIQCLEPKRSSTLRDGGPESGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CED1</accession>
    <name>A0A8H6CED1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001951</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151418.1">
        <property type="nucleotide sequence ID" value="XM_037292879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CED1"/>
    <dbReference type="GeneID" id="59330365"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="268" mass="30530" checksum="511C8820A6E47D9D" modified="2022-01-19" version="1">MPALMLAVCAHEASIGREEDADDKIFPYLFKPLKCEFIELVRGDELGGLAVIALSTADNFKTLKCQSGLRGQDRLRTFTQVLSVEHSYECFEYLPDRSLYHFNLSAPALDFLSRTLPLLSRLVIDLGDFIGYSPSWDQAYKKATKNNARYSHAPLSCVDGLNIVCAIARFQNLRHLTLHYRLQQDQIALMHPTPGCEAVRELFESTQRRKRGQAIVRLDVIFYTDSINVFGCVDRSWQIDPPTVSTTITILHLRQYTLWEGDRAPEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEK9</accession>
    <name>A0A8H6CEK9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6222014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151449.1">
        <property type="nucleotide sequence ID" value="XM_037292910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEK9"/>
    <dbReference type="GeneID" id="59330396"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="131" mass="14065" checksum="33BC78DF822D8FC3" modified="2022-01-19" version="1">MATTSTSPPAAKLLPSDKPSDIRYDPRTHSYTWPTFSDHRSGNAKWHLPRTRQYGNCVIDVRLNRGVRSEVSSWGFVARAASNVITECVQWGGGRGGKGATGETESIEVTVYSVVADGAGVLNGTLVVASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEM3</accession>
    <name>A0A8H6CEM3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001769</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151236.1">
        <property type="nucleotide sequence ID" value="XM_037292697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEM3"/>
    <dbReference type="GeneID" id="59330183"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13778">
        <property type="entry name" value="Aar2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13777">
        <property type="entry name" value="Aar2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.550">
        <property type="entry name" value="Aar2, C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007946">
        <property type="entry name" value="AAR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033648">
        <property type="entry name" value="AAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038514">
        <property type="entry name" value="AAR2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033647">
        <property type="entry name" value="Aar2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038516">
        <property type="entry name" value="AAR2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689">
        <property type="entry name" value="A1 CISTRON SPLICING FACTOR AAR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689:SF4">
        <property type="entry name" value="PROTEIN AAR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05282">
        <property type="entry name" value="AAR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20981">
        <property type="entry name" value="AAR2_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAR2 N-terminal" evidence="4">
        <location>
            <begin position="7"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="AAR2 C-terminal" evidence="3">
        <location>
            <begin position="180"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="390" mass="44279" checksum="F241DF3448321F9A" modified="2022-01-19" version="1">MDTTLTAAVLLLGLPPGILCGIDLLSFTTTAQFRGLKDLPLGWHFVFTSETTSLSVRDGFWFHVPTTVTGSIPLIIRKWDTPGGSLLPSDPDSFRVRLPLLWEKNLSPYRQSADKETKAETGDWASLTEHVSPRLLGHLTQNEDWKITTASCAKEDRDEIPGLNAEDFGDEERELGMLGIDLKRTWRKGAIGRERTEAALDSSWALNDVVERWQPRDQKDKGEWGSVVLGQMEACFLMVLTVANYSCLEEWKRCVGLVLTCKRAVREKEEWFGTFLVLLRRQMERCEDVEGGLFDLRDEGGAYLKSLLKGFKMTLGKVFEDAEGEQVRKEMEELETFMKSEYGWELGDEFVRRGMLELEDGEQVEMEMEEMDGEDERGEYAPVVVDLADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFH2</accession>
    <name>A0A8H6CFH2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151959.1">
        <property type="nucleotide sequence ID" value="XM_037291597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFH2"/>
    <dbReference type="GeneID" id="59329078"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034485405" evidence="2">
        <location>
            <begin position="23"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="163" mass="17831" checksum="478E00586149677A" modified="2022-01-19" version="1">MHMSWGFYIDSVLLFLSVLAQASVLPPGDQHSSPDKSQSPTIQPQHPSSQGSYKSHHVPLRSAGSMIKRDDEQSDDLRIRTITIVTGSIPIAAAAQSLEMFYNAILFNALAPWCSQPPQQALVMTMGPLQLTMVVVFNSGVPRGIPWAFKYAGDDSTGIYRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFR0</accession>
    <name>A0A8H6CFR0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chalcone isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000689</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151988.1">
        <property type="nucleotide sequence ID" value="XM_037291626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFR0"/>
    <dbReference type="GeneID" id="59329107"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016872">
        <property type="term" value="F:intramolecular lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016087">
        <property type="entry name" value="Chalcone_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016088">
        <property type="entry name" value="Chalcone_isomerase_3-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036298">
        <property type="entry name" value="Chalcone_isomerase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47284">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47284:SF3">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16035">
        <property type="entry name" value="Chalcone_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54626">
        <property type="entry name" value="Chalcone isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chalcone isomerase" evidence="2">
        <location>
            <begin position="129"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="359" mass="38856" checksum="D7B27AE1D7B991C1" modified="2022-01-19" version="1">MPPSLARQLSSRSHAYSTQPTNPQARNRPNYRIAIICGLTAVAATWAVTYRKPERLDAPPNNRRDALTSGARVEVTGQDHGPDTDQVPTGTSTVPYFPRSIWLPRSGGASEEGKSPALPAGIGAAQEEEEYQLLGLGIRKVSFLRIQVYVMGLYVAKGDMGRLQKELLRASVGSGASTLVQGEKEDLKKTLLDGEGSTEVWSQVLKEGGVRSAVRIVPTRDTSFSHMRDGWVRGIDLRGKGKEFEGEDFTVAVDQFKGMLGGRGSVGKGKVLLLGRGANGALRAWVEEDAGRADSKTLTRKGGRMTLLGTVKDERISRLVWMGYLAGQNIASEDARRSVVDGLIEIVERPLGTVETQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFS6</accession>
    <name>A0A8H6CFS6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152053.1">
        <property type="nucleotide sequence ID" value="XM_037291691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFS6"/>
    <dbReference type="GeneID" id="59329172"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42084:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PPK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42084">
        <property type="entry name" value="YALI0E26631P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="456" mass="50463" checksum="90D751853A390245" modified="2022-01-19" version="1">MSSDLQKEFGYPEVNPWRGAASQSPANKASADEDDFGDFEDPENEENVTHTPRNSSPPYHTVRYPVGSEWSGTQRLSMDQEYLQSFIHSSTTEVQEVPSNHDDWGDFSQQSVMFDADMEATRQKQEADRTSVDQRRRLEAPTNTQLHPKTSTKAFTPPSPIPAITGANISQRSQGSGKARHQSSTDDSKRRVETTKATNLEHPTIDDEPWVDFEAAEAAKSGPKPSNGPISRGFGATEASNLGPPPSNIPPPSILLSAIATIFGSLATNLKKSTSYQPLDQPSTLSQLRALLSTLRAAARILAGRKLRWKRDNLLSQSMKIGPAHSGKAGGMKLAGVDKAESRREDQEAAEALQVWKQQAGPLRSMIATANGQMSEIERFKIPEIADNMLIRQGKPSEGIVTAPKCCFLCGIKRDERVVKVDFDVEDSFGEWWVEHWGHFDCVAFWENLKAFLQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFX3</accession>
    <name>A0A8H6CFX3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152184.1">
        <property type="nucleotide sequence ID" value="XM_037291822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFX3"/>
    <dbReference type="GeneID" id="59329307"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="256" mass="26722" checksum="7BE5FF7C3A89DE57" modified="2022-01-19" version="1">MSGIPIHTEDPISPAEASGVTPQTAYNSNAITPSQPAKTTAPSPYAYAPARPGAAAPTPTRSTPQSSSHGPPAPQPGAAPVPPPPTTTAQPSLPPPPKAGEKASSSEHYAPVRPTPAQPQPYPPQMSQPTVDPPFQALPPASTTSTSMRPSFDPSAPPGSVSSPPDMPIRASLEHPPGYVQNPFASDMTPDQRFAADENRSGTLPSLGYTDNAQVPRPGSEEDRTAWGTAKKWAMEKGERASKLGEEVWEKFGAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGX4</accession>
    <name>A0A8H6CGX4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152392.1">
        <property type="nucleotide sequence ID" value="XM_037292030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGX4"/>
    <dbReference type="GeneID" id="59329516"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="466" mass="51011" checksum="810A4FFC46B9CA52" modified="2022-01-19" version="1">MPESKQDSLPDMLKLMTDVEIKNDVQVMLGPDGPALYTWGDVDTYMKKNADSLEPDVVRSVMQKLRDAYKMRKFNEELTRALEAVQIGTIAGGETDTTDNDDNDTDDTDNQILTPLSRNSDVENAGRDENPMDHGLRATTVGQELEHLLPEAPPVRDYRDANGFDHGFAMREATPASATYVILPNDKKTSNKDTSPNEGGSGAAAACKTSKKDQKPGLMVKGTPPPPLISNQQSPYLIRAEPGQRTVLEQKQTANSQPLSFPLHTTLLSNISIHNSFCQHSSHVTWFVVGRGKNVVVVSKTEMFAEQAGGKEREGVSAILAKSSTQWALQLLKEKNFYGIRSSAPLETLTKIILVNAVARNARGSTTAPAMHEGRESLTSSEVDEDDSGYEADADEPKEDSKGAGEADNADEPDSSTDGTDTDTDTSHNWYVSDADYLASCLESLDYEREGEYQDGDDVVDYRYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH40</accession>
    <name>A0A8H6CH40_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152640.1">
        <property type="nucleotide sequence ID" value="XM_037291205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH40"/>
    <dbReference type="GeneID" id="59328685"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40434:SF1">
        <property type="entry name" value="CUPIN TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40434">
        <property type="entry name" value="CUPIN_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="94" mass="10606" checksum="47F31767E5396543" modified="2022-01-19" version="1">MAPPSRKESEAQVRSWGFSHVFSWADGPNAHYPPHSHHCLTTHLIRRGTLTITYPKDETPTKETFGVGSRVDVEAGRVHEVWMSDEGCEYIIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHL8</accession>
    <name>A0A8H6CHL8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152866.1">
        <property type="nucleotide sequence ID" value="XM_037291431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHL8"/>
    <dbReference type="GeneID" id="59328911"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="385" mass="43188" checksum="6489CDF41B50C29E" modified="2022-01-19" version="1">MAARGSIQQNPSQVAGTFQPAPPVASGVIPGPSAPLQMGQAGTVPWVNVMYASDSRWENPSTWEKVDLLQEALKPSIRSFTRVTSRSPPSVPGSPWQSYASQLDILQRESDRFWSADRRPGAPPKLAGLAAWRGGVLMVREAGFHITEEMTEEFMHAKLRGYRHRDGSPGDKFIEQTHNQFQNVLLAADTRRGRIRAQAVERRGQQGVSENTTAILDNIVARDPERFLAWLSTMGYLYFTMKDHNPYAFSWEDWCFWKAPRSFEVCCKQASAVKRPLLGPGAIDRAKYGPRGDRPTITLAEAKKGNKKALDFQDTADGIEVSLKEKAQYYIIGGEATSREAAAHYHAIRGEDLWLFEPMNSVKEAYNDLSRQREAEVEPSFRCLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHN1</accession>
    <name>A0A8H6CHN1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL23 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000231</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152606.1">
        <property type="nucleotide sequence ID" value="XM_037291171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHN1"/>
    <dbReference type="GeneID" id="59328650"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000035">
        <property type="entry name" value="60S ribosomal protein L23a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005633">
        <property type="entry name" value="Ribosomal_uL23_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03939">
        <property type="entry name" value="Ribosomal_L23eN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL23 N-terminal" evidence="7">
        <location>
            <begin position="17"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="156" mass="17236" checksum="EDF725C2F4E74CC8" modified="2022-01-19" version="1">MAPKAASKKAPIGGKHNAKASLAAKSALKGAHSKKARKVRLTTSFHRPKTLQLSRSPKYPRKSVPHEARLDHHKVIINPLNTESAMKKIEENNTLVFICDVKANKRQIKEALKKLYDVDCVKINTLVRPDGSKKAYARLTPDVDALDIAATKLNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIT2</accession>
    <name>A0A8H6CIT2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010792</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153277.1">
        <property type="nucleotide sequence ID" value="XM_037301643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIT2"/>
    <dbReference type="GeneID" id="59339182"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23814">
        <property type="entry name" value="UEV_AKTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="12"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="320" mass="35295" checksum="048F7EC671247246" modified="2022-01-19" version="1">MTSLHQLPWHHNIQQHALLEYASLKQACPEGIYLSLKPGASTQWSGVLFVRKGPYAPAILRFLVSFPPDYPTLPPVITFISDIFHPLVAPLTTYTYTTGSSSSETVSATDEERLPPGGFSLRHGFPHWFGRSQKSAKFSATSSRNVSDAHPGAANQREDVYKPPPPEDLSSRESSPRSATFRMSHVRTRPRRSIQPSQQTKITEVLEYMKSAFDDEIALDTLPLEAAGNSGAWKAWRAYRRSNEADSKLVDSAPSLHKQDEWSWDGVWEERVRKGVDASIAESTLYGGTSGGDDLIRFVDVGNDLTEAVRAGVVSHVGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ66</accession>
    <name>A0A8H6CJ66_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase III subunit RPC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_011004</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC9 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153487.1">
        <property type="nucleotide sequence ID" value="XM_037301853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ66"/>
    <dbReference type="GeneID" id="59339394"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038846">
        <property type="entry name" value="RPC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561">
        <property type="entry name" value="CALCITONIN GENE-RELATED PEPTIDE-RECEPTOR COMPONENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="129"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="157" mass="16920" checksum="1F06E663257D57C0" modified="2022-01-19" version="1">MKILESQSAVLSNYEVYAHLTAMRATPRTAPRKPSNVDTVLKELTDYLSPPPASRSRVPRYQNPPYGAAALRALVAGLRPYDLTKSEVLMIANLRPEGLGLLDCVVEECDERFTAERQDEIVKIVGDVLGREGDGDGVDGDGVDGVDGEGGHEDGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJT6</accession>
    <name>A0A8H6CJT6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_010042</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Regulates also the sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153715.1">
        <property type="nucleotide sequence ID" value="XM_037300898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJT6"/>
    <dbReference type="GeneID" id="59338434"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058348">
        <property type="entry name" value="DUF8035"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26118">
        <property type="entry name" value="DUF8035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DUF8035" evidence="13">
        <location>
            <begin position="228"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="328"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="567"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="746"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="502"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="338"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF26118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6224848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="893" mass="99873" checksum="3DE529BC60993E92" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJV2</accession>
    <name>A0A8H6CJV2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_010119</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-X family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153792.1">
        <property type="nucleotide sequence ID" value="XM_037300975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJV2"/>
    <dbReference type="GeneID" id="59338511"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00141">
        <property type="entry name" value="NT_POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.210.10:FF:000005">
        <property type="entry name" value="DNA polymerase IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000010">
        <property type="entry name" value="DNA polymerase lambda"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.110:FF:000005">
        <property type="entry name" value="DNA polymerase POL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.210.10">
        <property type="entry name" value="DNA polymerase, thumb domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002054">
        <property type="entry name" value="DNA-dir_DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028207">
        <property type="entry name" value="DNA_pol_B_palm_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018944">
        <property type="entry name" value="DNA_pol_lambd_fingers_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037160">
        <property type="entry name" value="DNA_Pol_thumb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022312">
        <property type="entry name" value="DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002008">
        <property type="entry name" value="DNA_pol_X_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276">
        <property type="entry name" value="DNA POLYMERASE TYPE-X FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276:SF29">
        <property type="entry name" value="DNA POLYMERASE TYPE-X FAMILY PROTEIN POL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14792">
        <property type="entry name" value="DNA_pol_B_palm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10391">
        <property type="entry name" value="DNA_pol_lambd_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00869">
        <property type="entry name" value="DNAPOLX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00870">
        <property type="entry name" value="DNAPOLXBETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00483">
        <property type="entry name" value="POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81585">
        <property type="entry name" value="PsbU/PolX domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRCT" evidence="13">
        <location>
            <begin position="185"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="78"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6224925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="723" mass="80638" checksum="5AD2619EAC7AA17B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKH6</accession>
    <name>A0A8H6CKH6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nuclear pore complex protein An-Nup82</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010420</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154090.1">
        <property type="nucleotide sequence ID" value="XM_037301273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKH6"/>
    <dbReference type="GeneID" id="59338811"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037700">
        <property type="entry name" value="NUP88/NUP82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257">
        <property type="entry name" value="NUCLEOPORIN NUP84-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="16"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="804"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="847"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="758"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="817"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6225223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="905" mass="100173" checksum="FAF0F96DD11775A8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKJ8</accession>
    <name>A0A8H6CKJ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153954.1">
        <property type="nucleotide sequence ID" value="XM_037301137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKJ8"/>
    <dbReference type="GeneID" id="59338674"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013268">
        <property type="entry name" value="UTP16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08297">
        <property type="entry name" value="U3_snoRNA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="578" mass="61654" checksum="CA1F437629B3A2F7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKS2</accession>
    <name>A0A8H6CKS2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6225054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037301104.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="130"/>
        </location>
    </feature>
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        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
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            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
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            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
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            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="461" mass="52913" checksum="324DAD9001BE108F" modified="2022-01-19" version="1">MRDKLEKQFPEIIEQLTNNQKTTVEHYEKELRLALDHRFEFLQTSVVDLNARIATKDLENFQLKNQLDQASTVAEKLRTSEEEYKQQTLEVRNQLSTFEEQSNHKVLQLRNQLSALAEQSTQERTKLRNQVTQDSSDAHELRICNAELHHQLSQYSIAAESFSSFNNELLNQLDQTITVLSELKTERLELRKQQSHDAELSTMLSARLQQASEDTELLRVRNTELDARLQQTSKDGELLKVRNTDLDTRFQQAFEDIELLRARNTQLSINLQQSAHDAMQLSTRDDDLSIQRFQYSNEPNSPPPFPGHTTTPARRTSLAAFSPDNLVSKKRVIGKRASKGQGSRASIDSDSTQSRSPSEVSLVSEPSLQRPMVVTTHASYPRDATTIGCVRIDAQDVEQGRWAPGVSGDLQELIKDYLKWAEGRKKSHLRPKVDQLKLSDIQCHWHELARHDRQRLITLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLB1</accession>
    <name>A0A8H6CLB1_9LECA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="59338027"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="256" mass="29464" checksum="B3838CF95901718C" modified="2022-01-19" version="1">MAELLQTLHEQYRTEHYGPFHTRECYPWEYDAEAQQCLERGNKPSYFVTEEDCKFFQQQAKILQDRAAYIRQEWARQEATLQPSTKPVTQPVTQSTTQLATQQATQPEHSVEQEEELQQANAENTAEKPSDNEATIIKNMALLSTISHQSNQPVQEEEPILQANAENTAEKPSDNKATIIKNTAQLSKIPRHMLSTVPRQIITQQAENTTATWPVIPTALIWYITAMTSQEEDLLRRLQPIGPLQEEHRTMHIWTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLZ7</accession>
    <name>A0A8H6CLZ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009809</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037300668.1"/>
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF0">
        <property type="entry name" value="U2 SNRNP-ASSOCIATED SURP MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="SURP motif" evidence="3">
        <location>
            <begin position="337"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="CID" evidence="4">
        <location>
            <begin position="477"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
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        </location>
    </feature>
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        <location>
            <begin position="285"/>
            <end position="326"/>
        </location>
    </feature>
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        <location>
            <begin position="434"/>
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        </location>
    </feature>
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        <location>
            <begin position="535"/>
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        </location>
    </feature>
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        <location>
            <begin position="678"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
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        <location>
            <begin position="434"/>
            <end position="447"/>
        </location>
    </feature>
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        <location>
            <begin position="678"/>
            <end position="714"/>
        </location>
    </feature>
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        <location>
            <begin position="715"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="763"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="792"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="822" mass="89330" checksum="FA6B0AAF5845C6B2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM49</accession>
    <name>A0A8H6CM49_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009661</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000007">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037300522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM49"/>
    <dbReference type="GeneID" id="59338056"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000060">
        <property type="entry name" value="Ubiquitin conjugating enzyme (UbcB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="6">
        <location>
            <begin position="3"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="154" mass="17370" checksum="3007A4B785F3509C" modified="2022-01-19" version="1">MATAQKRITKEYADIQKKPLDGMRISLPSEADIHQWQILIDGPKGSPYEGGVFNLHLSLPMEYPFKPPTLSFKTKIYHPNVTNDDKGSMCLGMLRADEWKPSSKIDAVLSFAQGLLSEPQPDDAVEGGIAREYKENKKEFVKNAKAWTKQYAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMD1</accession>
    <name>A0A8H6CMD1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008989</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154676.1">
        <property type="nucleotide sequence ID" value="XM_037299850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMD1"/>
    <dbReference type="GeneID" id="59337384"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000001">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056263">
        <property type="entry name" value="RPN11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23594">
        <property type="entry name" value="RPN11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="9">
        <location>
            <begin position="33"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="313"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6226123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="336" mass="37435" checksum="E1633F17F03F0E35" modified="2022-01-19" version="1">MDRLNSLMRGSMMGGSSAPPGADSNLVDNSETVYISSLALLKMLRHGRAGVPMEVMGLMLGEFVDDYTVRVVDVFAMPQSGTGVSVEAVDPVFQTKMMDMLRQTGRPETVVGWYHSHPGFGCWLSSVDINTQQSFEQLTPRAVAVVVDPIQSVKGKVVIDAFRLINPQTLMMGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSIGINYRKTGLEENMLMNLHKHVWTEALQMDDFREEGGKNQDKLKRLVGLAEGYEKRVKEETELTKDQLKTRYVGKVDPKKHLEDVGQQLIEDNIVSVSRQMIDKEASVAKTNGANGHQDGHQTGSMDVDEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMM4</accession>
    <name>A0A8H6CMM4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154610.1">
        <property type="nucleotide sequence ID" value="XM_037300762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMM4"/>
    <dbReference type="GeneID" id="59338298"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034334442" evidence="1">
        <location>
            <begin position="19"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="176" mass="19246" checksum="53DF486C80C54854" modified="2022-01-19" version="1">MPWILLLEVLILPAFALCAVVPQPSDTYDLLQGPVISPALYFPENLSFATNDTSFDEVLKLRCDPVRYGRNLNVGSCRKVFNFITQDDTQTVFAERSTVQPHDLNLPFRATSSDGLCYVQPILAEGAVTARASPREVGNAAYTLFQECVVKRGMGGIAGDIGQLNPVEILGHLGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMM5</accession>
    <name>A0A8H6CMM5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009173</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154860.1">
        <property type="nucleotide sequence ID" value="XM_037300034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMM5"/>
    <dbReference type="GeneID" id="59337568"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF32">
        <property type="entry name" value="PROTEIN (MNN4), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G03790)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034149896" description="LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein" evidence="6">
        <location>
            <begin position="28"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="7">
        <location>
            <begin position="134"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="7">
        <location>
            <begin position="224"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="314" mass="35998" checksum="A6B63984F7140AD8" modified="2022-01-19" version="1">MILRSPCWLSLALAFTVLLLQYGLVKGDADIESVRIHHGYEDMSGRGGDPKAKYWHESVFHPHYDGRFTDHTLPYEEQKANLSLLMQSYLSSMSDIGAETWIIHGTLLGWWWNQKVCSPNHIAISSPDSTTQIMPWDSDIDVQMTNTTVHMLASYYNMTIHTFDIGDDEKRDYMLEVNPKYTDPSYDDTLNVIDARWIDVETGLFIDITAVRPHQSKKGIICSKDQHEEKVQDLFPLRDSLFEGQAVKIPYNYARLLTQEYGNAALTKAEYSDHRFNSTSMEWEKFMTQADEDGDGVKYPDGSVEEEDGDEEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMS1</accession>
    <name>A0A8H6CMS1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154825.1">
        <property type="nucleotide sequence ID" value="XM_037299999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMS1"/>
    <dbReference type="GeneID" id="59337533"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="131"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="214" mass="23795" checksum="450E40E6AEB10EA1" modified="2022-01-19" version="1">MVLEKSAPEERDLKGSDHGKIKNQKSPIGIPCMSGRPPWANQRCSGPKRLGGVANHSSGIADPISDIIAPILKFKPTWLQIQRQLGNGMQLSDTTAGRRIAQDLSGKIRKYQESFDSTLAEIQQSHEHKIRAALQEQANKFKQDLAEAKHDREMLKADFEKRVKADNERRRKLAEEQRSPGDWQSGLKNAAVAMGMASLAGFAAYKYLNGTTMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNB5</accession>
    <name>A0A8H6CNB5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009438</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155125.1">
        <property type="nucleotide sequence ID" value="XM_037300299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNB5"/>
    <dbReference type="GeneID" id="59337833"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107">
        <property type="entry name" value="NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107:SF7">
        <property type="entry name" value="RE15974P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="269" mass="28170" checksum="EA87939C676ED946" modified="2022-01-19" version="1">MATTIPAPLIGKVAIITGASRGIGAAIAIELARKGAAAIAITYMGNLPAAENTLAKIRATSPQTTCIAIKADLLSPTFGEDVVKAALSDLKTPHLDIVINNAAISDMRYQEPFEEMTYEGFERMMRGNGWGTIQLIRAALPHIRPGGRIINISSTSSKRPNLDPVMAYGMSKAALDSITRSLAVKYAAEKKITINSVSPGPTETDLLAQTLKGDEKLAASVAQGSTAEKRTGTPEDVAGIVGFLASDEARWVNGNHVPASGGRAGIMDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNP8</accession>
    <name>A0A8H6CNP8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Urea active transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009307</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154994.1">
        <property type="nucleotide sequence ID" value="XM_037300168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNP8"/>
    <dbReference type="GeneID" id="59337702"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015489">
        <property type="term" value="F:putrescine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000006">
        <property type="entry name" value="Urea active transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="571"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="635"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="650"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6226441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="666" mass="71134" checksum="2D9FB7E985592CE7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP13</accession>
    <name>A0A8H6CP13_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kynurenine 3-monooxygenase</fullName>
            <ecNumber evidence="11">1.14.13.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Biosynthesis of nicotinic acid protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Kynurenine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">BNA4</name>
        <name evidence="14" type="ORF">HO133_008434</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-kynurenine + NADPH + O2 + H(+) = 3-hydroxy-L-kynurenine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:20545"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the aromatic-ring hydroxylase family. KMO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.9" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP13"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00328"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004502">
        <property type="term" value="F:kynurenine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070189">
        <property type="term" value="P:kynurenine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000129">
        <property type="entry name" value="Kynurenine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01971">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027545">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028:SF2">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0274">FAD</keyword>
    <keyword evidence="2 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 11" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 11" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="13">
        <location>
            <begin position="9"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6226993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="511" mass="57699" checksum="B2908F325B7F0321" modified="2022-01-19" version="1">MAEVHKAQKVVVVGAGPVGALAALYAANRGDDVHVYELRADLRDPSTTPLNFTKSINLALSERGINSMRQSQSVGLLEEVLKETIPMHARMIHGRRKGGELFQESQAYDANGRYIQAVDRASLNKRLLEALEKLPNVTLHFNHKLTGADFDRKTAWFEQHNNAKKTDHAQNPTQNDALWDDQKRATEISVNFDLIIGADGAHSATRYHMMKFARLDYQQEYIDTLWCEFHIAPRSPDSSFAIDPNHLHIWPGGSFMFIAIPSLDKSFTCTLFAPASHFSSVSENPEQKLVKFFHQNFPGVCPELISTSDLQKQFRENPHLPLISIKCKPYHYKSSVVILGDAAHAMVPFYGQGMNAGLEDVRVLFDILDKHGIYDAGTSDHRSRNEAREAALDEYTSQRAPDAAAINDLALRNYEEMRSGVQSPLYKMRKWMEETVNVYFPSLGWRTQYTRVSFENQRYSEVEKAVHRQGRILMGVVGLSLVGIGGLTAFGGVRRDYWKNLGTTWLSQVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPC6</accession>
    <name>A0A8H6CPC6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155451.1">
        <property type="nucleotide sequence ID" value="XM_037299450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPC6"/>
    <dbReference type="GeneID" id="59336980"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="26299" checksum="38B96B6F16894814" modified="2022-01-19" version="1">MDRNPQPGLEVTSHDGPEIVLQPDGNKYYTGSSELNEFALSQPDKPIHSVSKTSRNKAFWALAVIAIVCLAVALGAGLGAGLSTKRKSSTSSSPIITVTQSGNIFTATQAPAITSTAPSTSTTTSPVCPAANGSSYIATNKPSSSLGPEYVIPDTSLTYQILCNTNFRTNNITAAVDMQIINNVTSLKECLDACALYSFQTPPNNFPALGCSGAVWNHNTLVPDCWLKRNIILSESDEESGIEAAVLLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPU1</accession>
    <name>A0A8H6CPU1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008846</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155710.1">
        <property type="nucleotide sequence ID" value="XM_037299709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPU1"/>
    <dbReference type="GeneID" id="59337241"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002054">
        <property type="entry name" value="DNA mismatch repair protein MSH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011184">
        <property type="entry name" value="DNA_mismatch_repair_Msh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF21">
        <property type="entry name" value="MUTS PROTEIN HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005813">
        <property type="entry name" value="MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0469">Meiosis</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="7">
        <location>
            <begin position="652"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="826"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="831"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="845"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="886"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="895" mass="99727" checksum="7B86E561E651C3E7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPW6</accession>
    <name>A0A8H6CPW6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155733.1">
        <property type="nucleotide sequence ID" value="XM_037299732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPW6"/>
    <dbReference type="GeneID" id="59337264"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="183" mass="20521" checksum="6C59A123D6533980" modified="2022-01-19" version="1">MSTAPQFLPVWNEESAKAADDRKVEGEAKQTRQAWNDWGELLREGTQRRPAKRSDAPHFRLKLTHKPSGRSVMLCFMGWTRNQPPNAAERPVYQYPETPSGSPHSFSMHLIHVTWGYVHKSPDIVDAEKSWSSTEGSYPLGVNWKVDESPTGKNLNVDTSRMSTAAAAKFATLRIIKTNATAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPW8</accession>
    <name>A0A8H6CPW8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155750.1">
        <property type="nucleotide sequence ID" value="XM_037299749.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337281"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034455716" evidence="1">
        <location>
            <begin position="23"/>
            <end position="1582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1582" mass="163714" checksum="010D383F802D03E0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQT0</accession>
    <name>A0A8H6CQT0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156084.1">
        <property type="nucleotide sequence ID" value="XM_037298748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQT0"/>
    <dbReference type="GeneID" id="59336277"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="409" mass="44477" checksum="BCA5CD148BC970AE" modified="2022-01-19" version="1">MSRSHRRPSDYSRGSSPRIDRNLAVTEASFQNLGLNETAGAQYQSGPSSYLLPDQRSRRPSYPRSELSSGYLQPDYVSSSYTRAASPASFSALENSTSFGTTLLDPSDYQLFSDTGTFSTRASPFSTSVLGRTDMGSYPYPSDTMTSYRDTSSYGPSRSPSQRGYTNPSTEASLSRSMSGPSGPSILPSSAYGGFRGLEREVARQRQRKLHESRRQSKLLLRRTMLPSTPYARPGEADTESGSIDYLLAHSGSSYDSHSAYGRTPSTSSAQGSYLTGVSSSPYDNPYTTAYGTSGVGSHTMYSSSSQNPSLSTTSSAIYGYRGDRRGSGVPSNMEPVVIETPKKPQCWDHGCNGRQFSTFSNLLRHQREKSGTSSKSVCPRCGAEFTRKTARDGHLAHDKCKARSSSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRQ6</accession>
    <name>A0A8H6CRQ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007759</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155965.1">
        <property type="nucleotide sequence ID" value="XM_037298629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRQ6"/>
    <dbReference type="GeneID" id="59336156"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000266">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034923143" description="Carboxylic ester hydrolase" evidence="3">
        <location>
            <begin position="23"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="88"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="632" mass="68342" checksum="DCC0A89DD7D2E45A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRU6</accession>
    <name>A0A8H6CRU6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156439.1">
        <property type="nucleotide sequence ID" value="XM_037299103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRU6"/>
    <dbReference type="GeneID" id="59336632"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="235" mass="24846" checksum="D1452390294053A4" modified="2022-01-19" version="1">MEELRKIGTAAEEDGIKYRSGMELALVALAVQLSMVLVALDMSIIAIAIPRFLWPRLGPLVLLSATVFPIGGAVIVTLILLCQTPAHVKSVLATHSEPFSMPDTPGIITIGGAQLCYIPTSLPWSGEPASPSLGDAANVVDALVGSYKASLTVLFVASRGASDHRSETPSTKQRTGAARDASIFFTIARPYKLPSSFQTVNGVSPFESGMRTLGTVPSLSSPSKKWLILESLLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRZ4</accession>
    <name>A0A8H6CRZ4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008011</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156215.1">
        <property type="nucleotide sequence ID" value="XM_037298879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRZ4"/>
    <dbReference type="GeneID" id="59336408"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010961">
        <property type="term" value="P:intracellular magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12829">
        <property type="entry name" value="Alr1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000008">
        <property type="entry name" value="CorA family metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000014">
        <property type="entry name" value="CorA family metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044089">
        <property type="entry name" value="Alr1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535">
        <property type="entry name" value="MAGNESIUM AND COBALT TRANSPORT PROTEIN/MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535:SF51">
        <property type="entry name" value="MANGANESE RESISTANCE PROTEIN MNR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="812"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="847"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="459"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="689"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="191"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="480"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="511"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="689"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="875" mass="97984" checksum="1AA89F55C1186D4B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7F0</accession>
    <name>A0A8H6F7F0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_006899</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000028">
        <property type="protein sequence ID" value="KAF6217429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146864.1">
        <property type="nucleotide sequence ID" value="XM_037297795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7F0"/>
    <dbReference type="GeneID" id="59335299"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF461">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="544"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="112"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6217429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="587" mass="64901" checksum="2A3C63897635E846" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F804</accession>
    <name>A0A8H6F804_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006456</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147479.1">
        <property type="nucleotide sequence ID" value="XM_037297354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F804"/>
    <dbReference type="GeneID" id="59334857"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046824">
        <property type="entry name" value="Mss51-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032717">
        <property type="entry name" value="Mss51_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069">
        <property type="entry name" value="GH20023P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069:SF1">
        <property type="entry name" value="PROTEIN MSS51, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20179">
        <property type="entry name" value="MSS51_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13824">
        <property type="entry name" value="zf-Mss51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial splicing suppressor 51 zinc-finger" evidence="1">
        <location>
            <begin position="18"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial splicing suppressor 51-like C-terminal" evidence="2">
        <location>
            <begin position="169"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="376" mass="43403" checksum="1C097BFF89DC1641" modified="2022-01-19" version="1">MPLSPHDPEARKGAQSTQPPAFVRFECPDCGTPVSCSEEHWADDYESHTEICDVLREINEDDHDLRSGRFFPEFEYPGPQMEEALVNMTNWDTYLYTREYRAINEERPLRQVTKLLTYPVTIGSILSELSPYNIRRNGRITTEGLKSLSALRYTLHPPRTGADGTIKGLRLNPPPVRIFILGARAESSLPREVWQQLSFIFPRVAFHLIFIGPESMTNRDAEFPLPERTPGNPFGAVVEDRISHEMKISTFVEYYHTLHQAGLFHPYDPYFDCFVLFHPGLGHPASSHEWADTLPQLLETKVPIISTGYTEWDMERDRKWVEDKVGGEMDILMEPGENKFRSLRWDLNEFDPTDVSCGNWGVWAFRGKRYETTMKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F817</accession>
    <name>A0A8H6F817_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_006501</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10 11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10 11">Cytoplasmic vesicle</location>
            <location evidence="10 11">COPI-coated vesicle membrane</location>
            <topology evidence="10 11">Peripheral membrane protein</topology>
            <orientation evidence="10 11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10 11">Golgi apparatus membrane</location>
            <topology evidence="10 11">Peripheral membrane protein</topology>
            <orientation evidence="10 11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147524.1">
        <property type="nucleotide sequence ID" value="XM_037297399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F817"/>
    <dbReference type="GeneID" id="59334902"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051645">
        <property type="term" value="P:Golgi localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09254">
        <property type="entry name" value="AP_delta-COPI_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14830">
        <property type="entry name" value="Delta_COP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000003">
        <property type="entry name" value="Coatomer subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027059">
        <property type="entry name" value="Coatomer_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121:SF0">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="13">
        <location>
            <begin position="276"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="154"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="243"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6218089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="515" mass="57043" checksum="6166A0737DE30FEF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F855</accession>
    <name>A0A8H6F855_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148170.1">
        <property type="nucleotide sequence ID" value="XM_037296188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F855"/>
    <dbReference type="GeneID" id="59333683"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034588220" evidence="1">
        <location>
            <begin position="25"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="224" mass="24782" checksum="B0458F6984D79127" modified="2022-01-19" version="1">MPSLHSYPSIAALLSSMLFQVIQCAPATSPLPSQPIYSLPPLRPSLPLNASDNGNCASTTKYPSWNSNDWVIEDCYIAVQQLYFREVLEHPDVAYEFVALGVSPTRFSLESQRTPRKYIFRSCVLTIMMLDWFQPGQLPAGTKYNRAQTDVSTYRDIWSAARSIETACIVSKSAGWLMTGKNSAMGVFVWSTNSQLNEITGGFPVHSLGLNNSDIMILDGVQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8K6</accession>
    <name>A0A8H6F8K6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148183.1">
        <property type="nucleotide sequence ID" value="XM_037296201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8K6"/>
    <dbReference type="GeneID" id="59333696"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_bL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="Ribosomal_bL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680:SF9">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27013" checksum="4D289D102C374BBA" modified="2022-01-19" version="1">MASTAPTLRQSSCWKRLLRPSRQQKTCVRCLSTALATTPSQFLPPPPPRLRSPFRILNDTTHTLSKKIKTYPPPPSHRAKHPFAVADWQAYHQKQLDPTGSRTRLFDRNNPDVPRVGDILLVAFKTGDPFAGVCLSIKMRGVDTGILLRNKLHMVGVEMWVKIYSPNVMAIEVVKRAEKRARRGKLYYMRKPKHDKGSVEKEVEAYLKRRRLIRSGALGVKDPSLSAKNPASARSGVGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8L9</accession>
    <name>A0A8H6F8L9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine protease</fullName>
            <ecNumber evidence="11">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_005132</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase C54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148742.1">
        <property type="nucleotide sequence ID" value="XM_037296044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8L9"/>
    <dbReference type="GeneID" id="59333538"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="13">
        <location>
            <begin position="101"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="44"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="403"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="420"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6219307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="442" mass="49276" checksum="48CA5A68B73A51B0" modified="2022-01-19" version="1">MSSVDFGRYKRIVQAFWDPEPKNDNASVSCIWCLGREYVTRCTPNGSRDPESEAKRKEPALDGQDCHQDAKRVKLAATDRTSAGEPEAQSHDSEADRGWPSEFLDDCESRLWFTYRSSFTAIKKSSDASMTLSVRLRSLGDQGGFTSDTGWGCMIRSGQSLLANALVMVRLGRDWRRGRRQDEERQLLSLFSDDPNAPFSIHRFVEHGASACGKHPGEWFGPSATARCIQALSHEYQGTGLKVYVNGDGADVYEDSFFKLAKASDGTFNPTLILVGIRLGIDRVTPAYWEALKASLQLPQSVGIAGGRPSSSHYFFGIQGDQFFYLDPHVTRPALPFHKNPTDFTEEEIGSCHTRRLRRLPLKDMDPSMLIAFLIKDEEDWKSWRRAIGTASGKPVVHVADEEPSLHGHGHGAVRKSAVDDVETLDDEDEGDDGEMVERPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8R0</accession>
    <name>A0A8H6F8R0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005441</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148333.1">
        <property type="nucleotide sequence ID" value="XM_037296351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8R0"/>
    <dbReference type="GeneID" id="59333847"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF17">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="6">
        <location>
            <begin position="65"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="217"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="44853" checksum="145E3659F20D873F" modified="2022-01-19" version="1">MNGTNGTDDHKFEELMQTLNGINADTFSNDGKRIQTLLAAYALVSRLETPWDFVLRLCMGQPALGAALKVSKDLQLFDKWHERGDGAMTREQLAAMVSCDPNLLFRLLRHLAANHVLQETSIGVFKPTKLSISFTQSVFGEWINHLYDATIPCFFKMPMYLAQNGYQNPLDPSNGVFQYAKGWKGDMFHYYESHPVEGESFNHVMGGVMANQAGWVDIFPHNAILESDSDSESPLVVDVGGNIGHDMEKFRQLHPETASRLYLEDRPEVVKHSLCPEPVNKIGYDFFMPQPIKGARAYYMHGVLHDWSDEPARKILEMQRGAMKPGYSKLLIHDHIAPEALAHPHTTAYDLTMMVMVAGVERSEAHWSTLLKSAGYKFLRVWTSPLAVQGIIEAELEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8Z6</accession>
    <name>A0A8H6F8Z6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005578</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148469.1">
        <property type="nucleotide sequence ID" value="XM_037296487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8Z6"/>
    <dbReference type="GeneID" id="59333984"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="178" mass="19994" checksum="C4EC9BCE55620CF2" modified="2022-01-19" version="1">MYQFKTSEGHTTNLNPIIQELLAWAYIVLITVPTAAKTQCHEVYCLDKEGKTCVLCEEGERRFEMNQLKAKRSVRDAQVAAALAARADTMATGKVRWDDKLPTVDKLAKTAAKKTQQALKTGNSQNMEYGKAEKIWKGPKGKSPAKVQHTEAIKADETFQAEQYKAKVKPKLRIQRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F983</accession>
    <name>A0A8H6F983_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004150</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149116.1">
        <property type="nucleotide sequence ID" value="XM_037295069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F983"/>
    <dbReference type="GeneID" id="59332559"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF14">
        <property type="entry name" value="GABA PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="339" mass="37033" checksum="65FF7876F8DD86C7" modified="2022-01-19" version="1">MELSEPNPIDEKSNAQTEVTSTSDVDFSTESVQEQKDGASHDVYDMSRMGKRRELRRIFRFISIVGSVMKPLGYLVGWSSAFGWVTGCPSAAQLTATLVRGLVLLRNPNASVDALWQTTLFIMLFLVAAAALNIFCARQLPVTEGIFVVVHVVGFFVFMIVLWVTSDHAPAKQVFTQFQDNGGWGNTGLSALVGITTPLWCFLGPDAGAHMSEELKDASRVLPSAMMWGSFFNALLGLLMLITLCFCLGPDWQDNVLGLTNPTQTGISIIQVLYNSTDSMIAATTFMTTILILLSMVGTITCIASSSRQVWAFARDKGFPFSSYIEYGQVGIYPSTPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9G1</accession>
    <name>A0A8H6F9G1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003246</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149550.1">
        <property type="nucleotide sequence ID" value="XM_037294169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9G1"/>
    <dbReference type="GeneID" id="59331657"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="49"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="353" mass="38243" checksum="DAD92772CDFBAEDA" modified="2022-01-19" version="1">MSTSSTPSHSPSPFTPRSTPTFSYRIGASFSAKGRRFDPKTDVFTFNTQTQITSEQDVFTGRPNSGQDAFFVSKAGNSSNVAFGVADGVGGWADQGIDSADFSHGLCQGMARIAGELHSPDKKDLWPQYILGNAYQEILREGKIGGGGSTACLATGDEDGNLKVANLGDSGFIQFRLNAVHHFSNPQTHAFNTPFQLSVIPPKILALSRLFGGKQLSDSPEDSNDTSHEVRHGDVLMFATDGVWDNLSTRELLKIVSRQMTGCHAWETGEKGTTVSEMIHDLTQDGGIPKQYENNLQALLAVNVVGEAKAVSMNTKRDGPFAKEVQKFYPHENYHGGKVDDICVIVAIVVRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA11</accession>
    <name>A0A8H6FA11_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149653.1">
        <property type="nucleotide sequence ID" value="XM_037294272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA11"/>
    <dbReference type="GeneID" id="59331761"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prion-inhibition and propagation, HeLo domain"/>
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        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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        <property type="entry name" value="GPIID_WHD"/>
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        <property type="entry name" value="HeLo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="AMELOGENIN"/>
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        <property type="entry name" value="FUNGAL STAND N-TERMINAL GOODBYE DOMAIN-CONTAINING PROTEIN-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14479">
        <property type="entry name" value="HeLo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22939">
        <property type="entry name" value="WHD_GPIID"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ANK_REPEAT"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034047242" evidence="3">
        <location>
            <begin position="24"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="domain" description="Prion-inhibition and propagation HeLo" evidence="4">
        <location>
            <begin position="5"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="6">
        <location>
            <begin position="235"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase winged helix" evidence="5">
        <location>
            <begin position="526"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="824"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="878" mass="97991" checksum="19AA9C194F5E4082" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA69</accession>
    <name>A0A8H6FA69_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150076.1">
        <property type="nucleotide sequence ID" value="XM_037293998.1"/>
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    <dbReference type="GeneID" id="59331486"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="214" mass="24238" checksum="F2BE03EC645ED802" modified="2022-01-19" version="1">MDTARAQVSKSSERSIGQTHGEPGTKVTLPTGENISKYQQLRRVHIPGFPATTERLPEGIETEDIIKQWPNHLWGPLLLQVIAHWSPKQISNLSGGRLKPNSIVKRVKAARIQAGDTQGQAKSPKKGGENPRKRKRDTDAPPRIREEALNTEQLDVESEASRQFRISQMEIEDAVKAKDPNYELRQIGLKKMPRLDRILIDQAFEERKVDVKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAK7</accession>
    <name>A0A8H6FAK7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_002498</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150253.1">
        <property type="nucleotide sequence ID" value="XM_037293424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAK7"/>
    <dbReference type="GeneID" id="59330911"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046970">
        <property type="term" value="F:histone H4K16 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF9">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="9">
        <location>
            <begin position="208"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="379"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="539" mass="59708" checksum="B46F391073BC2433" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBE9</accession>
    <name>A0A8H6FBE9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151620.1">
        <property type="nucleotide sequence ID" value="XM_037292201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBE9"/>
    <dbReference type="GeneID" id="59329687"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="276" mass="32615" checksum="C74AA7D22FB9884D" modified="2022-01-19" version="1">MAKPLTAMQAALQDPKHLRGCAEEYHYEAQRRSWLRPFIIQNISPCSEMLEVPNQSHKRVAKTIAQLDPDDCRQFCWKDCPIYCNTSVNAIVHVEILEFVYREWFPHRDRERDISFDSFFGKFMRFYPPVDMAEVVSFHQRLCERLAMLESDVLAPEYTKPHPKHGHVRERGLFKLRETFCNLFIVADHKDWRERGVLLVCKDEETATAHGINETKEEDLSREKSLEVEDMRSACVFRMSVKRAMQAVVFRDGDRGRRRREYNEAFEEYLGSEDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBZ0</accession>
    <name>A0A8H6FBZ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Dihydroxyacetone kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_001300</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glyceraldehyde + ATP = D-glyceraldehyde 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13941"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dihydroxyacetone + ATP = dihydroxyacetone phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15773"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dihydroxyacetone kinase (DAK) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151649.1">
        <property type="nucleotide sequence ID" value="XM_037292230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBZ0"/>
    <dbReference type="GeneID" id="59329716"/>
    <dbReference type="UniPathway" id="UPA00617">
        <property type="reaction ID" value="UER00669"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050354">
        <property type="term" value="F:triokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019588">
        <property type="term" value="P:anaerobic glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.340:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1180.20:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10440:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase, DhaK subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10440">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.20">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012734">
        <property type="entry name" value="DhaK_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004006">
        <property type="entry name" value="DhaK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050861">
        <property type="entry name" value="Dihydroxyacetone_Kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02361">
        <property type="entry name" value="dak_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629:SF14">
        <property type="entry name" value="DIHYDROXYACETONE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629">
        <property type="entry name" value="TRIOKINASE/FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02733">
        <property type="entry name" value="Dak1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82549">
        <property type="entry name" value="DAK1/DegV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="DhaL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51481">
        <property type="entry name" value="DHAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DhaK" evidence="15">
        <location>
            <begin position="10"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="DhaL" evidence="14">
        <location>
            <begin position="392"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-hemiaminal-histidine intermediate" evidence="11">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="58"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR612734-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR612734-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6222214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="592" mass="62247" checksum="4845B5A571069F58" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC34</accession>
    <name>A0A8H6FC34_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Na+/H+ antiporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_001479</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fungal Na(+)/H(+) exchanger family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151828.1">
        <property type="nucleotide sequence ID" value="XM_037292409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC34"/>
    <dbReference type="GeneID" id="59329895"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000015">
        <property type="entry name" value="Na(+)/H(+) antiporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013928">
        <property type="entry name" value="Cation/H_antiporter_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08619">
        <property type="entry name" value="Nha1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="411"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="26"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Alkali metal cation/H+ antiporter Nha1 C-terminal" evidence="14">
        <location>
            <begin position="459"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="468"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="767"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="861"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="476"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="511"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="541"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="571"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="589"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="613"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="634"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="691"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="892"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="983"/>
            <end position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6222393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1106" mass="121786" checksum="DFE4315AF321E3E7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCF2</accession>
    <name>A0A8H6FCF2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152114.1">
        <property type="nucleotide sequence ID" value="XM_037291752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCF2"/>
    <dbReference type="GeneID" id="59329234"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF112">
        <property type="entry name" value="DUF821 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_2G14740)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="2">
        <location>
            <begin position="244"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="508" mass="57905" checksum="6C604C7D151EEB87" modified="2022-01-19" version="1">MAVEHLRSIEQDPRNGALNGVCVAKSVINRGQPAQNGQNAYLVLAVRENRVSSQRVPRRKSCRTEQSPGSEEAVKKKSYSEKAELQAVFRELTRLVTNTNNRAPPVKSNSKWAPGVPSKESNTATLHQTAKASPAADKQETTTTEWTFNATRDGDNLALSEEQCNIAFPKQYTDIHNNVANLTSNPITLSDLDEQDQHDQRIRAIIYDGELYIISRHGNMWVQNGVATAHALQRALSAFPDRKSLPNIEFFVFTFDIVGENKAVWTYTKPAHDTEFKNQWLMPDFGYWAWPHAHIPSYNHIRNEIRRVEEQVAFEDKTPQLVWRGNKNTAPERPRFLEATDGKDWANVSASGSAYLTLWDHCRFKMLMDISGRSWSGKGKYIQNCESVYVTHTPKWLKTTTYALEADGAEQNFVQVKDDWSDLEDKVKEVLETPRTAERIAKKGVEDMRDRYLTPAAEACYWRALIRGYGRVSFEPQLYEKDGSTLRGVPFETVAISPSIDLEKWWDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCX2</accession>
    <name>A0A8H6FCX2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152932.1">
        <property type="nucleotide sequence ID" value="XM_037291497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCX2"/>
    <dbReference type="GeneID" id="59328977"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054289">
        <property type="entry name" value="DUF7025"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411:SF3">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22942">
        <property type="entry name" value="DUF7025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="466"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="677" mass="76097" checksum="E1CA0DB608751A59" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE13</accession>
    <name>A0A8H6FE13_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153904.1">
        <property type="nucleotide sequence ID" value="XM_037301087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE13"/>
    <dbReference type="GeneID" id="59338624"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="563" mass="58535" checksum="8041DFEFBA6CE51D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE39</accession>
    <name>A0A8H6FE39_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_011070</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Siderophore biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NRP synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153553.1">
        <property type="nucleotide sequence ID" value="XM_037301919.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59339460"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043041">
        <property type="term" value="P:amino acid activation for nonribosomal peptide biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031169">
        <property type="term" value="P:ferrichrome biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="A_NRPS_SidN3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19542">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Nonribosomal peptide synthase SidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000033">
        <property type="entry name" value="Nonribosomal siderophore peptide synthase SidC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.30:FF:000014">
        <property type="entry name" value="Nonribosomal siderophore peptide synthase SidC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000024">
        <property type="entry name" value="Nonribosomal siderophore peptide synthase SidC"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="5"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45527:SF2">
        <property type="entry name" value="FERRICROCIN SYNTHETASE (NONRIBOSOMAL PEPTIDE SIDEROPHORE SYNTHASE ) (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45527">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="7">
        <location>
            <begin position="846"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="7">
        <location>
            <begin position="1974"/>
            <end position="2050"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="7">
        <location>
            <begin position="2531"/>
            <end position="2607"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="7">
        <location>
            <begin position="3087"/>
            <end position="3160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1961"/>
            <end position="1980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1970"/>
            <end position="1980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="3657" mass="402826" checksum="427B9E65D6EEB711" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEF8</accession>
    <name>A0A8H6FEF8_9LECA</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010246</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153918.1">
        <property type="nucleotide sequence ID" value="XM_037301101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEF8"/>
    <dbReference type="GeneID" id="59338638"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF52">
        <property type="entry name" value="SINGLE-STRANDED DNA-DEPENDENT ATPASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="374"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="785"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="949" mass="106332" checksum="993FDCFAA8516A41" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEL9</accession>
    <name>A0A8H6FEL9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154361.1">
        <property type="nucleotide sequence ID" value="XM_037300513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEL9"/>
    <dbReference type="GeneID" id="59338047"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF7">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="47"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="30852" checksum="9153100C88630636" modified="2022-01-19" version="1">MFSFRSRNRVSKTVKPLKRSVGERSKLKTKQKLDSPMVGSTMISPIITLVVGPDLRLFAAYEHALLKSPYFAKRLRDQFYETSSKRLKLPDMLPEIFACVLEYLYTDDYYPRLCKSKHHHSWELEDAQDTLNSGGRGRVPSTIYHPGVDGNYLLTDTVVYCAAEKLGLEELKRLALIKQGLRFCALDDVLIRSARYAYANTLDTDHRLRPFFIEMIIQGRKRFIRSPTMQMEMGLGGSKFFFDLFVAMCRHVDDFVERTNKSSPRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEU9</accession>
    <name>A0A8H6FEU9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010441</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154111.1">
        <property type="nucleotide sequence ID" value="XM_037301294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEU9"/>
    <dbReference type="GeneID" id="59338832"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="169" mass="19080" checksum="A6DA5C51C007CF0D" modified="2022-01-19" version="1">MLLIPEGEPFGELAPVLDESVIGFPSDIRPARPGVKEMLEPARFELGQTGIPVPKLVVFAQSLLDTNNGVDLEDLVNGMNLSMEWGEEYLELDGSTDVAWANWKAEALEREGKSLHGWDGSPEKRREIWQSTVSADGEKWGQGWKYNAACETRFWRRGQRHPRRRKGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFE7</accession>
    <name>A0A8H6FFE7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_008997</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154684.1">
        <property type="nucleotide sequence ID" value="XM_037299858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFE7"/>
    <dbReference type="GeneID" id="59337392"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000003">
        <property type="entry name" value="C6 transcription factor (Leu3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF21">
        <property type="entry name" value="REGULATORY PROTEIN LEU3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="69"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="627"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="633"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6226131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="708" mass="78917" checksum="59AA5B14677C0EA4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG38</accession>
    <name>A0A8H6FG38_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009444</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155131.1">
        <property type="nucleotide sequence ID" value="XM_037300305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG38"/>
    <dbReference type="GeneID" id="59337839"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF56">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="2">
        <location>
            <begin position="136"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="478" mass="54128" checksum="3FA27F3196E39E3F" modified="2022-01-19" version="1">MASKSTENDQDIRSDPPTMTALTLQEMKDGVPAPSENDSIHSSSAHSDTSTLNHDQESYDLFKLRVERLCQILWPPQKSIRQALSNSKTATRMRASKFFRLIIRSQKAPLIERLRGGDYNRIVGITLPLSKGEESRNRNLILRVPRWGQGPTERVVATLDYLRRKSTIPIATTIAKDFSKENPLESPYIIQNRIPGSNLEVLWTELNHSQRCTVACEVGRVIKSLLALESPVTGHVEAYSNGMGSTEPHTIVPFDLKSAGGDQFEEPEQQASTIKGNPRESQTTLDFFKCQIGRWRAVDVARESPMLDRTVGLWDGVLKVVEEMNDLGLFIPRSHCLCHVDLHPRNIMASIKPSGCIQITGILDWDEAVIAPKFVNCEPPGWLWGFDPDDLPHDDLPTWPYERPGANEVPSTLQSQELKRIFEHHAGSEYVSLAYHEHYRMSRGLFRLAVFGLTSSENYSTAERIICDWERLRQSLMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGI3</accession>
    <name>A0A8H6FGI3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008484</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155351.1">
        <property type="nucleotide sequence ID" value="XM_037299350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGI3"/>
    <dbReference type="GeneID" id="59336880"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="571" mass="63964" checksum="694349DC15233BA4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGV1</accession>
    <name>A0A8H6FGV1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kinesin-like protein KIP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_008788</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for assembly of the mitotic spindle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155652.1">
        <property type="nucleotide sequence ID" value="XM_037299651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGV1"/>
    <dbReference type="GeneID" id="59337183"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="16">
        <location>
            <begin position="251"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="739"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="887"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="632"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="220"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="739"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="905"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="933"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="951"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="330"/>
            <end position="337"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6227344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1026" mass="111481" checksum="1533384560F329C9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH32</accession>
    <name>A0A8H6FH32_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155820.1">
        <property type="nucleotide sequence ID" value="XM_037298484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH32"/>
    <dbReference type="GeneID" id="59336011"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16577" checksum="183CF30F7AB8E5B9" modified="2022-01-19" version="1">MGRTKGKTDLSGPKKGVLVALSNLNNASNRQIAKAYKCDEKTVRNTKKRVSEAEKENIDPLSSEVHQRRPQSGRPLAINERLLRRLIRHATKNRFQRRKPWVTIAREVGCMASATAINAAFSRAGYGRYPPRYKPPLSPEQKQKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH64</accession>
    <name>A0A8H6FH64_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156361.1">
        <property type="nucleotide sequence ID" value="XM_037299025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH64"/>
    <dbReference type="GeneID" id="59336554"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140053">
        <property type="term" value="P:mitochondrial gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF39">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="762" mass="87474" checksum="B38D7A5A5817D879" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHD5</accession>
    <name>A0A8H6FHD5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007597</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155803.1">
        <property type="nucleotide sequence ID" value="XM_037298467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHD5"/>
    <dbReference type="GeneID" id="59335994"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="36"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="401" mass="44462" checksum="02D4B94DCB195DA2" modified="2022-01-19" version="1">MSGNGSNSNAYDQTRVPGLYAATITLAVLQTIAVVARFAARSISAATFWWDDYTIVLALRQAPGLTVPQILDFGLCVCYWVPIRICNFGRHTQAYGGPVTEGDLIIFFKNFTEILHLTFLPEKSQLALQILYFATACAIKTSLILLYYRLFGVVRWFRLILAVMWLIVLAYFVADTLVAVFECSPIAYYWDKAIKGGSCIDQDAFYRWNGVANLLIDFSILVLPMMMVWRLNLDTRSKIILSGVFLLGTLACVASIVRVWAFNLANFDDFEYTITTAVIWTVVEQSLGIICACLPTLRPLLSRFGLFTIKSTYVFNSKNTNSRSGAAIGLSHLSSRPGFSPSTDVSNAAGFARLDEENGTLEHGSLTTHAVRMPSGDVPQVSRGIPKGQTVEQHYDEADNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI90</accession>
    <name>A0A8H6FI90_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007116</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156644.1">
        <property type="nucleotide sequence ID" value="XM_037298009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI90"/>
    <dbReference type="GeneID" id="59335516"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050335">
        <property type="entry name" value="ERT1_acuK_gluconeogen_tf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659:SF4">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="170"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="34"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="226"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="455"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="512"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="455"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="527"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="585"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6229002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="616" mass="67707" checksum="42A7CD70AB068BA4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI98</accession>
    <name>A0A8H6FI98_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AA9 family lytic polysaccharide monooxygenase</fullName>
            <ecNumber evidence="5">1.14.99.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Endo-beta-1,4-glucanase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycosyl hydrolase 61 family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007137</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Lytic polysaccharide monooxygenase (LMPO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and/or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[(1-&gt;4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1-&gt;4)-beta-D-glucosyl]n-1 + [(1-&gt;4)-beta-D-glucosyl]m + acceptor + H2O.</text>
            <dbReference type="EC" id="1.14.99.56"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM).</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.56" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156665.1">
        <property type="nucleotide sequence ID" value="XM_037298030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI98"/>
    <dbReference type="GeneID" id="59335537"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21175">
        <property type="entry name" value="LPMO_AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049892">
        <property type="entry name" value="AA9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353:SF32">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034833489" description="AA9 family lytic polysaccharide monooxygenase" evidence="6">
        <location>
            <begin position="22"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Auxiliary Activity family 9 catalytic" evidence="7">
        <location>
            <begin position="22"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6229023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="465" mass="48752" checksum="FBB474A7204BC407" modified="2022-01-19" version="1">MLVSHVKIVILVAGAAQLARAHTAFTNFFVDGVDQGDGTAVRMSNNIQQATFPLSSVTSPDMACGVNGETGVSRVASVNAGDTLAFEWRDWPDASQPGSIDPSHKGPCAVYMKKVDNSAASNNAAGDGWFKIMEDGYDSTAGKWCTEKLIPNDGHLAATIPHDLAGGYYLVRPELLALQQADKTPADPQFYVGCAQIFLSSSGSADPLNTVSIPGYINMSTPAMTFNIYDQPMKLPFPDFGPPLYTAGSSKRDLEARAMVQTIGLKPDACVMENVNWCGFIPSSYTNETGCYAASQNCSVQATDCYNSAGPTGSKNCFNWENFCTQIRNGCGAGNYNGPPSISSFMPAPLPKLDLAAVVAASGPMAISNSAPIEASSTVSMEAYDSATTAMPAPAAMAVGPARQGCVVLLMDTAVRLRITVARGVSLNSATALEPQSASAGTCDSTDTSFVVADFISEVGKIQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIH3</accession>
    <name>A0A8H6FIH3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007291</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156817.1">
        <property type="nucleotide sequence ID" value="XM_037298182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIH3"/>
    <dbReference type="GeneID" id="59335690"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="32"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6229175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="417" mass="48384" checksum="F4AE40831FAB7461" modified="2022-01-19" version="1">MAAGTSRILRYVLFAFFGLMILYFISSSSIPKPEMPGGSNFERWKSKKDKSNASVGKSPAGPPSSGEKDLAAESGSSKLTGKLETSSGLSTGAKMNATFVSLTRNSDLWDIADSIRNVEDRFNRNYHYDWVFLNDQPFDETFKKVTTAMVSGTARYGLIGKEHWSFPDFIDQDKAKAVREDMAERKIIYGDSISYRHMCRFESGFFFRQPLMNEYEWYWRVEPSIKLFCDIGFDPFVFMAENKKKYSFVISLFEYAETIPTIWDSAKKFMKLHPEHIAEGNAVDWLSDDGGESYNHCHFWSNFEIGNLNWLRSKAYLEFFEVLDQDGGFFYERWGDAPVHSIAAGLLLKKEEIHFFDDIGYWHVPFTHCPTSEQKRLENKCHCNPKDNFDWKGYSCTTKWFEKNELKYPEGWEDQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIN8</accession>
    <name>A0A8H6FIN8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="4">5.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_007384</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:23285"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156910.1">
        <property type="nucleotide sequence ID" value="XM_037298275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIN8"/>
    <dbReference type="GeneID" id="59335783"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000012">
        <property type="entry name" value="Isopentenyl-diphosphate Delta-isomerase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018427">
        <property type="entry name" value="Isopntndiph_ism"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="13">
        <location>
            <begin position="83"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6229268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="263" mass="30073" checksum="F873FAE1F0435945" modified="2022-01-19" version="1">MGSTTTETQTITAENVIRLFPDVDTHLATSHRVPTSDADLQGYDEEQIRLMEEVCIVLDENDVPIGSASKKVCHLMTNINEGLLHRAFSVFLFDSKNRLLLQQRASEKITFPDMWTNTCCSHPLGVPGETGDELEAAVQGVRRAAQRKLDQELGIGVKGVPLDKFRFLTRIHYKAPSDGKWGEHEIDYILFIKADVDHTPNPNEVKDTRYVSEAELKAMFKNDSLKFTPWFKLICESMLFEWWEHLDQGLEKYTGESQIRRML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK16</accession>
    <name>A0A8H6FK16_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004263</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157183.1">
        <property type="nucleotide sequence ID" value="XM_037295182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK16"/>
    <dbReference type="GeneID" id="59332672"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6229926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="455" mass="51478" checksum="E5159BC03B484EC7" modified="2022-01-19" version="1">MGSTPQQAEELRIAGESCKKRFHDLLEASKRRDGYILNVLSERRTLIHSQRYGMWTANVDILETGPHSLDFRLNEKKILRDYVLVITIATTPRASITRKLYAETSDSSPDVVEEDFKSQDPPLAVKTIYNALADEEEICDEIIRDRLNSLFEIASEIPSNEASTPIEHATCAVQNVQNALTPGDEQPNSSTTGGIYPTPLNSARTAVSGVTTQPGYSVEKPTASKKDRFSMKRLFNTSNRHERVPHRRPRRRRLHRIAAQFVQNAWIETHDRTIEDSYRKQTEVDGRQVILEILDTAGEEQSSMRQRNPALAAKRELYIKSGQGFLLVFNTTSLSSLDKLSSLHDRIVRIKNDPLTPLVVVGNKCDLKEDRAVSQAKALAVAQSWNAPYHETSARGRTNVDEAFVDLCRQVIRRDVAAEQTSVMETEDRKWMGRLHRGAKEMKKKGSVRRDCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK43</accession>
    <name>A0A8H6FK43_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157227.1">
        <property type="nucleotide sequence ID" value="XM_037295226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK43"/>
    <dbReference type="GeneID" id="59332716"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055530">
        <property type="entry name" value="DUF7104"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23397">
        <property type="entry name" value="DUF7104"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="46059" checksum="7D9649D9EE9523BA" modified="2022-01-19" version="1">MSQTLVNKAAANGTASTLRLLFDRGGVTTGPVLRCAAGNNPEGAKMMSLLLAEAENWMIMGEMTEMMKIAAQSAFEGPSIMKRLLEHAVDLLITEDVLVTAARIGPRNELFELFSGRDWEMTEEVLEAMMSHLASEKTLQLLLDRLEGLEITGKVLLAAASNRSFGDQLVGLLLDRVNLLDIVNPLLVEAAGNDTFGLELILLLQRRVGKVDVTQEVMERAARKGPMRTMTFISDHTSAPITQAVVVGALMSDRLEMVQFLLDRAIDLPVTRTMVHAAAQHSRAECLALIWERAWRAEVTEDIFKDLAQAAAENVVWPGKNLRFLLDVVEDIVIGPEALISIIRTDFDSVPLLDFLIDRGIDLQITHDVLQAAAGNFRYHRSLMTFLLERSDKAMLNDKLFRAAAGSGRVWVLQVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK62</accession>
    <name>A0A8H6FK62_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cysteine synthase 2</fullName>
            <ecNumber evidence="4">2.5.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cysteine synthase-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">O-acetylserine (thiol)-lyase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">O-acetylserine sulfhydrylase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_004340</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157259.1">
        <property type="nucleotide sequence ID" value="XM_037295258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK62"/>
    <dbReference type="GeneID" id="59332748"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000096">
        <property type="entry name" value="Related to cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="16">
        <location>
            <begin position="76"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6230002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="425" mass="45806" checksum="DAD825C4CF8772D3" modified="2022-01-19" version="1">MENRPKLYAITAFVAGILLTLGYKDFYPDLEWWFWRRRKANRTVAGAGLQDGGQINLEDHEKNGPTTMDVPEGIEACIGNTPLFKIKSLSSETGCDILAKAEFLNGGGGSPKDRVALGIINLAEEQGLLTPHSGDTIYEGTVGSTGISLATVCRARGYLAYICMPSDQATEKSSLLLKLGAVVERVPPAPIVDPMHFVNRARTLAAEHTADSNRLGRGYFADQFENEANWQAHYDGTGPEIFRQCGGNLDAFVAGAGTGGTIAGVARYLKPRLPDVKIVLADPQGSGLYNRIKYGVMFATTEKEGTRRRSQVDTIVEGIGINRVTANFEAGRELIDDAVKADDEQALAMARWLVEKDGIFVGSSSAVNCIAAVKIAQQLGPGHRIVTILCDSGTRHISKFWTRAGTIGGKTDSKLEDVIGTQSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKC0</accession>
    <name>A0A8H6FKC0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">P/Homo B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_004462</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157380.1">
        <property type="nucleotide sequence ID" value="XM_037295379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKC0"/>
    <dbReference type="GeneID" id="59332870"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007323">
        <property type="term" value="P:peptide pheromone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000026">
        <property type="entry name" value="proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000005">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF14">
        <property type="entry name" value="NEUROENDOCRINE CONVERTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 14" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="741"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="17">
        <location>
            <begin position="485"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="656"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="810"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="688"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="811"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="827"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6230123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="846" mass="93090" checksum="8F438A2344C1386F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYH4</accession>
    <name>A0A8H6KYH4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcium channel YVC1-like C-terminal transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007166</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156694.1">
        <property type="nucleotide sequence ID" value="XM_037298059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYH4"/>
    <dbReference type="GeneID" id="59335566"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052971">
        <property type="entry name" value="TRP_calcium_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056336">
        <property type="entry name" value="YVC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35859:SF5">
        <property type="entry name" value="ION TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35859">
        <property type="entry name" value="NONSELECTIVE CATION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23317">
        <property type="entry name" value="YVC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="289"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium channel YVC1-like C-terminal transmembrane" evidence="3">
        <location>
            <begin position="270"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6229052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="378" mass="41916" checksum="0C031D1BC1951CC5" modified="2022-01-19" version="1">MAAAMPQTPSSGGPRTGQTDWQLDPPRIDQDEPLGEIVRQLSVYFIDAIEAPHTFEQLRTASVGHILKPLIKSLSESCHHPDIVSALLILKCHFASGDQDDHGINDTRGHACEIVAWRFLTHLSAAELIDYLLHELPPVSFDPESYDGVGTHPDNGDAAEHAALLSGRDPSATSTRGNTRTSSIAEEEPMASFVGLNALEIAAVAGAKKFLSQRVVQKTVDDIWNGHIIFWESLSVHSKKRAKFYNKARADPYCRLRVPKYQKSFEALFFALFLGLYYAVLVERNPHKITPVEALLYIWIAAFAYDEFGEFRDAGISFYVADFWSLWDIGIIGIGVAYMVSSESLYCLMSSLSDEQVMPASRDEQGEAARQTPPLSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZL9</accession>
    <name>A0A8H6KZL9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004335</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157254.1">
        <property type="nucleotide sequence ID" value="XM_037295253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZL9"/>
    <dbReference type="GeneID" id="59332743"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="237"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="328" mass="35949" checksum="F7454B7B1BDA08BF" modified="2022-01-19" version="1">MYYDLNVPYIANHGELQRTLAFLSELGYNTIALDFAVTGKVPAEISNQIPTPLPFTIPNSLHILRRCTLHLSDPSQNYRLQALNSAYDVLAIRPDNEKSLQQACQALECDLISLDLSTRFPFYFKHKTMANALQRGVKFEICYGPGILNSDGGASRRNLISNATQLIRATRGRGIVISSEAKRALACRGPADVVNLAVLWGLGQEKGIEAIDREARSAVVHAEMKRRSFRGVIDVVHGGEKPEREPERFAKGKQEKGKRKASVLEGDEAGAGNPTPKPISKREQKRQAKKARLDTGKISENKPKEASKGSIISPEDAPASGIPNGNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZR5</accession>
    <name>A0A8H6KZR5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157448.1">
        <property type="nucleotide sequence ID" value="XM_037295447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZR5"/>
    <dbReference type="GeneID" id="59332938"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="416" mass="44933" checksum="CC3C0BCA6F6739F0" modified="2022-01-19" version="1">MPSSPPTPTRRRKSSSIAIHLPFISASPQSYACSSIRPSNSRKSSHCSVASPSTPQQISSHNRSGYFESSNGLDDLVDPGSANGLGNLADELAEAFEEEDENARELGDEVSEGLYDEAEAIHHDHLKENERPMFRNGYQKGHATSIPPVRQATSDRALSPAKQSLRSRPTRENSQYDGSDYGDDSDLDGTHSISASLEARLAAVENLARRGTEANGSDTDEIVQRVTDSLKDLGSQAGVEDGAARLITAHTALTTHLSHQTRTLLTLTHPLVSPLSAPPDPGFIDDLLPLLTSLVLALPTPTSPTLASLRNLHASTADLTSILTYLSDTLHMMRQTTSLASRRLRAARELVIEMRRETEARETGIHWVEKGNWEARLAGRECARVCGEVVGGFEEVCASWRKRLAGGMAGVEVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5K7</accession>
    <name>A0A8H6C5K7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146967.1">
        <property type="nucleotide sequence ID" value="XM_037297766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5K7"/>
    <dbReference type="GeneID" id="59335270"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="346" mass="38664" checksum="3107860F11922C8B" modified="2022-01-19" version="1">MPSHEILALSREAKHDYSKGAPSSWYADSLQEGLVYGSHRARTAPTNASFLRTCRLINTEATPIFYGANKIIVYAEDNNDIFYWLLDIGERNRRAIRHLEIGWAYGVSIESGRGNIHGILEKIEDMGESEDDEIREHRQQLIDMVKRLEKKTIRLIVRTLHLLVTNQGLQSLSVYLPGVDGGDIWDLPNPNFYFAEELFSNSTTSVHACIPEALARMIGIKTLTIGYTKDIELAEEIARATGAKQLLIETMPEGNTLMLSAAEQAEWASRGWSLEGRTAKKALKPNTVEDYSGNGTEEERKNEVQKGYISEGYAEGGRLKASMLHDMGSEESSEVIDDSSSGQDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6F3</accession>
    <name>A0A8H6C6F3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147222.1">
        <property type="nucleotide sequence ID" value="XM_037297512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6F3"/>
    <dbReference type="GeneID" id="59335015"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="114" mass="12422" checksum="A9A98E393CB1383B" modified="2022-01-19" version="1">MATPSDLTSATQLATQSDTLQEEGTLQQANAENTAEKPSGSKAPSTKNTGLLSTLPCQIATQQAENTTAIWPVIPTALIWYIIAMTSQEEDLLRRSQPIGPLQEEVRTMHIWTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Z3</accession>
    <name>A0A8H6C6Z3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_006447</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147470.1">
        <property type="nucleotide sequence ID" value="XM_037297345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Z3"/>
    <dbReference type="GeneID" id="59334848"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01729">
        <property type="entry name" value="LSm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017132">
        <property type="entry name" value="Lsm7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037188">
        <property type="entry name" value="U6_snRNA_Lsm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="9">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6218035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="76" mass="8369" checksum="3B4D1583A0ECB51D" modified="2022-01-19" version="1">MVKFNGGREIQGTLKGHDQLMNLVLDDVQEMLRDDEGNTSTRSLGLIVARGTLLVLISPLDGSEEIANPFNNQEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C792</accession>
    <name>A0A8H6C792_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peroxin 22-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006343</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147366.1">
        <property type="nucleotide sequence ID" value="XM_037297241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C792"/>
    <dbReference type="GeneID" id="59334744"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="48093" checksum="15433B8A0816F01D" modified="2022-01-19" version="1">MSYSYERQRRQTYGASTRRTAWGYWLPLAVTVTVATAGLVAWVWNERRDDDDDDLAYRPPGQAGPPPSGPDYTNAPPPSGYGPPPSDYGNGPSPGFQGPPPGFEGPPAAQTPFAPGSDGRGAEESSIAARMSGALRRTPSPQQIFDGARRNVVAGVTAAGAAVGGALSSITEEGKGGYEDHSRWSEEAESQHGEPSRKGPELRDLEGSTSSRQTTLSTIKASGKRKTVAIVVSAETEYEHSEDAEYHQEHASILSHLPEHVDPDSRIFVLIYAPDLKQHPLANRQSGQPDVSLASSYSNIGQEDAQSQGEKSPDPESAGGRSHMFNTLYTQAQALVNKETEIMPFTTPTGYIQILKHLAPETVYLQETLSESGDAIAQIKDWVGHDIVIVVGESGHGGLVDSEDEHEEKEKWWHNDPQIGLGKGVEIVDGLRVGEDWRRRIGGHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7J1</accession>
    <name>A0A8H6C7J1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147850.1">
        <property type="nucleotide sequence ID" value="XM_037296669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7J1"/>
    <dbReference type="GeneID" id="59334168"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="321" mass="36216" checksum="9C982A8CA414830B" modified="2022-01-19" version="1">MLLDRLRPSSKLPRNTRRNPSDSAVLYEAQSSTNQSCRNKRFRVPRKNPQQARNSTAQARAILKFQTQISMASPAIALSSTPNPIIVSGEAGHELLSTENDMILSSLITNDRNAPKSRKGRSDRVATSVLSDDSYLPVSEESDKRCKRKRGADGDLFQKRAKENPAEDIPRMESNLKSQTELAEAKAIDSETETKRLGTTHFGPHNKQESTTSLVLSNLVQIDDMDCATRQARLKILITIIERGDELTEVEKAQLAELEAKGTRVYGRDLSRINVANMDIGMRKNWRNVLRKKVDRYNVLNEAEMKQVKDFGMLLYMKRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7Q3</accession>
    <name>A0A8H6C7Q3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147834.1">
        <property type="nucleotide sequence ID" value="XM_037296653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7Q3"/>
    <dbReference type="GeneID" id="59334151"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="76" mass="8123" checksum="0DD508A6840F3AD3" modified="2022-01-19" version="1">MPHGPKDWLDSFFSSHSQQSTSTAASTAYKTSTLTVPSSTSSMPPSNLRRVGMGRMALHGDLYAKAALDSVNGTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C808</accession>
    <name>A0A8H6C808_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005910</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147995.1">
        <property type="nucleotide sequence ID" value="XM_037296814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C808"/>
    <dbReference type="GeneID" id="59334315"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04318">
        <property type="entry name" value="EcAsnRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="8">
        <location>
            <begin position="153"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="514" mass="57514" checksum="6FAB60A20FBEA2D1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8D6</accession>
    <name>A0A8H6C8D6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005916</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148001.1">
        <property type="nucleotide sequence ID" value="XM_037296820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8D6"/>
    <dbReference type="GeneID" id="59334321"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107">
        <property type="entry name" value="NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107:SF7">
        <property type="entry name" value="RE15974P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="28201" checksum="C9407BD70A3DA304" modified="2022-01-19" version="1">MLQKLQNLSLLSKNCHPAEKMSGPLAGKTALITGGSKGIGAATSLLLAKSGANVAINYSSDSSAAEDIAKQIGHEQSLLIQGDAGSIPDIERMVSETVNKFGKIDIVIPCAGIMLLKNLEATTEADFDKSYHLNVKGPYFLCQKAAPHMPEGSHIVLISTTQCTATTVQAPYLLYNSTKGAIEQMTRVMCKDLAPKGISVNAVSPGPTGTELFYRGKTEQMLKMIAGFSPMGRIGKPEEIAEAIVWLSSGSSSWISGQILRANGGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C915</accession>
    <name>A0A8H6C915_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="12">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_005507</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="12"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148398.1">
        <property type="nucleotide sequence ID" value="XM_037296416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C915"/>
    <dbReference type="GeneID" id="59333913"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF134">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6218963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="356" mass="40159" checksum="D86918282B373643" modified="2022-01-19" version="1">MSSNRLDSIPLPTLDRPFIVELWPQFDKLYSHVVGHSANKFVFVPGATSLSTFTATVTMLITYYVTVFAGREFMKNREPFKLNGLFMVHNLALTIISGVLLALFTEQLLPTIARNGVFYAICDHRGGWTAPLVTLYYLNYLTKYVELLDTVFMVLKKKPLTFLHTYHHGATALLCYTQLVGLTAVSWVPITLNLTVHVVMYWYYFQAARGVRISWKKWITRLQIAQFVIDLGFVYFASYTYFSSTYFPNLPTAGKCAGEEFAAFAGMGIISSYLLLFISFYFATYKKDNAKGGRGRKKSRRLSQTATRAAIDMSRMEVPDVQRMEDAVHLGGHSNGSAKGKENGGITPRVTRSRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C921</accession>
    <name>A0A8H6C921_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148690.1">
        <property type="nucleotide sequence ID" value="XM_037295992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C921"/>
    <dbReference type="GeneID" id="59333486"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="235" mass="26811" checksum="0711AF68E4BBA003" modified="2022-01-19" version="1">MQHRDTHSSPEDEETSTRKRMLEQQQDKLVNALQELYDRNLNRRAWPGPPLQKTAKGIPLTHDILDRLGLLKLDDQGIYETFEEDTDVLRQKMTIKEEEHAYPTPTTTKSEFSPATPNFEPFVSRPFAGEKAHPARRFQPTPPMHSPEEDRCMTFPESSLNLGPSMNLDPASLQTPSQTWMQSASRYVTDMDYNYDVALSYNGMDLMQQKANPCLPMSAWNDDDIGPLGLDPILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9F9</accession>
    <name>A0A8H6C9F9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004939</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148549.1">
        <property type="nucleotide sequence ID" value="XM_037295851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9F9"/>
    <dbReference type="GeneID" id="59333345"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056687">
        <property type="entry name" value="DUF7785"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057199">
        <property type="entry name" value="DUF7877"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25009">
        <property type="entry name" value="DUF7785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25289">
        <property type="entry name" value="DUF7877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7877" evidence="3">
        <location>
            <begin position="33"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7785" evidence="2">
        <location>
            <begin position="453"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="565"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="591"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="657"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="696"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="772"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="796"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="837"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="857" mass="93112" checksum="E77C52E04203EB53" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9I7</accession>
    <name>A0A8H6C9I7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148898.1">
        <property type="nucleotide sequence ID" value="XM_037294851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9I7"/>
    <dbReference type="GeneID" id="59332340"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40788:SF2">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40788">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="693"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="878" mass="100445" checksum="5D1131B01BAAF3CC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9U7</accession>
    <name>A0A8H6C9U7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA(Phe) 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4)]-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004071</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TYW3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149037.1">
        <property type="nucleotide sequence ID" value="XM_037294990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9U7"/>
    <dbReference type="GeneID" id="59332480"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="10">
        <location>
            <begin position="14"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="289"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6219602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="340" mass="37209" checksum="F47A28B0126F51EC" modified="2022-01-19" version="1">MRQKAQVSPSFVAKKNAILALLSTPESTYTDLSPKGSVDAAIKPLIDRINALDGVVTTSSCAGRVTVFLEGRKQGKGYEERGPARNDGRFGKESEQAAVPGGKGMGGKWLFVSHEPVGIFKKDEGNENLTELLGLRSSGAQNEALALSGEANEMRLARFQFEPMILHIMTASLHRAQPILAAAINAGFRESGIQSLKNLDDRTAFPMVAIRTAGLAFESLVGLIPNRTQDREDGAEIEEHVTCIVSEEYLELLVNIANERFKTNAERIQRFEQDLFKREEGLGQAWEDSNCRQERKRAEGLEQQQTLRKAQVTQERGGPSPSDIDEGIDNAGLFSSSPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA50</accession>
    <name>A0A8H6CA50_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003665</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149275.1">
        <property type="nucleotide sequence ID" value="XM_037294587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA50"/>
    <dbReference type="GeneID" id="59332076"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="30640" checksum="268ACDD8DABAEA4F" modified="2022-01-19" version="1">MPRVFLITGTSTGFGANLVQEVLDRGDFAVATARKPDSLSFKGTSFHNYLAVKLDVTDPSNIQSAFAAALDKFGRIDVVVNNAGYGLAGPFEELSDTQIKTQMDVNFFGLIAVTRKAMEVMRDQKPSGGLIQQVTSIGGQRGVPNFSIYCASKWAVEGFTEAISQEVKPDWGIKFTCIEPGGFRTDWAGRSMAFPEKRHPAYDHIDVQKAMGARHGTQAGDPIKGAKAMYELAVMKDPPLRVVVGTDAYKAIMGKIDSYGENYKKYEKISNSTDVEGYKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA72</accession>
    <name>A0A8H6CA72_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149013.1">
        <property type="nucleotide sequence ID" value="XM_037294966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA72"/>
    <dbReference type="GeneID" id="59332456"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007250">
        <property type="entry name" value="HSP9_HSP12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04119">
        <property type="entry name" value="HSP9_HSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002590">
        <property type="entry name" value="HSP9/HSP12_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="100" mass="10846" checksum="9454F56F8BADB57B" modified="2022-01-19" version="1">MSDLGRKDFGDKAEEKFTPDSQKSLFDKTKEGVTDAGDKVAGSVQPSDDKSITQNLSDSTSTKPGEESYLDTAKKTLEDAVEYVEKTAGDVYNKVVDSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBE9</accession>
    <name>A0A8H6CBE9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149614.1">
        <property type="nucleotide sequence ID" value="XM_037294233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBE9"/>
    <dbReference type="GeneID" id="59331721"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039136">
        <property type="entry name" value="NUFIP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019496">
        <property type="entry name" value="NUFIP1_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13309:SF0">
        <property type="entry name" value="FMR1-INTERACTING PROTEIN NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13309">
        <property type="entry name" value="NUCLEAR FRAGILE X MENTAL RETARDATION PROTEIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10453">
        <property type="entry name" value="NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="560"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="560"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="122"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="306"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="375"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="499"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="655" mass="72295" checksum="5083768E5D8A3ED9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBS8</accession>
    <name>A0A8H6CBS8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149945.1">
        <property type="nucleotide sequence ID" value="XM_037293867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBS8"/>
    <dbReference type="GeneID" id="59331355"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034457774" evidence="1">
        <location>
            <begin position="17"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="237" mass="23516" checksum="5329BBC088ED8A43" modified="2022-01-19" version="1">MKHIITLSILCSAAHSAFTNISITFQSTLSQDITPAPQYPTATAGATTVTVIPTQVVIGDATLTRNAPGTLIAGTPVSFGSTALVIGTTTMPFATSGVAPIYTVAGQVIAAGPAGVAIAGTTTSNNGPAVTVSGTPVSLGSFGLVVNAASTFTIPTVQPLQSVFAVGGQSVTEQYGRIIFPSTQAVANTSTAATGTAAGPMPTGSTQSFVGAAVKARNNFKTAVVVLCAFVLVVPWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBW9</accession>
    <name>A0A8H6CBW9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003070</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150072.1">
        <property type="nucleotide sequence ID" value="XM_037293994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBW9"/>
    <dbReference type="GeneID" id="59331482"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000014">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF41">
        <property type="entry name" value="BRAHMA ASSOCIATED PROTEIN 155 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="7">
        <location>
            <begin position="161"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="438"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="439"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="556"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="714"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="560"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="741" mass="82143" checksum="C203AC3275783B47" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCQ9</accession>
    <name>A0A8H6CCQ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002519</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150274.1">
        <property type="nucleotide sequence ID" value="XM_037293445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCQ9"/>
    <dbReference type="GeneID" id="59330932"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08829">
        <property type="entry name" value="SPFH_paraslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050710">
        <property type="entry name" value="Band7/mec-2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032435">
        <property type="entry name" value="STML2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF10">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16200">
        <property type="entry name" value="Band_7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="4">
        <location>
            <begin position="84"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="425" mass="45760" checksum="A47A5D10AF998D73" modified="2022-01-19" version="1">MALREASTSSLTRTATALLQPSRPHFYSRLPTTGSTCLNSTVFPSRQILQPLSRRSASTSPFSYASTSAAPSSYFQRPSLPANTIIRFVPQQTAFIVERMGKFKRILNPGVAFLLPIVDRIAYVKSLKESAMEIPQQSAITADNVTLELDGVLYTRVVDAYKASYGVEDADYAISQLAQTTMRSEIGRLTLDHVLRERAALNTNITEAINEAARDWGVVCLRYEIRDIHAPDPVVKAMHRQVTAERSKRAEILESEGQRQSAINIAEGRKQSVILASEALKSEQINMATGEAEAILVRANATARGIDAVAGSLNQGQEAARGAVSLSVAEKYVEAFGRLAKEGTAVVVPGNVGDMGGMIASAMAVYGKISEGQAKSMAKGMIQAPESPESTAKSAADASVDAAMGKTDEVAKSVLQGFEDTRWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD42</accession>
    <name>A0A8H6CD42_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002672</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150426.1">
        <property type="nucleotide sequence ID" value="XM_037293597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD42"/>
    <dbReference type="GeneID" id="59331084"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189:SF50">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="159"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="903"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1023"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1066"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="431"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="491"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="565"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="708"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1153"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="496"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="517"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1153"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1185"/>
            <end position="1198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1209" mass="134515" checksum="361FEC6B86E6B92A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDE7</accession>
    <name>A0A8H6CDE7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2EXR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002421</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151000.1">
        <property type="nucleotide sequence ID" value="XM_037293347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDE7"/>
    <dbReference type="GeneID" id="59330834"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045518">
        <property type="entry name" value="2EXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20150">
        <property type="entry name" value="2EXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2EXR" evidence="1">
        <location>
            <begin position="39"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="313" mass="35349" checksum="41BEA434B0362707" modified="2022-01-19" version="1">MQDTKRLEHKSMLALQRIHNRIPTTETLPLRPAIPPRSRFESLPFELRLQIYSLLLDGKPQCCHLEKAVGGRSYSTPKLYPAILAVNRSISAEAYPVLYGENTFLFLGTTQIADHLNINHQFLRRLVGLPKIHDPSLLPKTSRHLIKHVAAAPLELARNDWVGRLLGLAPAIKTIEFDFWVGFASIPTLDASIPAIKSLIGASTQTFRIGTRDFDAYHASGFRFSRRRRVELPGMILAVVDLKGLDHVQEKSRLVHKALRFIIEIMGQRPPMPNNTIFPGQALVSVEYRVFLHKDFAGFWSSDEFTVEAETQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDR1</accession>
    <name>A0A8H6CDR1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynactin subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002219</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynactin subunits 5/6 family. Dynactin subunit 6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150799.1">
        <property type="nucleotide sequence ID" value="XM_037293146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDR1"/>
    <dbReference type="GeneID" id="59330633"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027777">
        <property type="entry name" value="DCTN6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13072">
        <property type="entry name" value="DYNACTIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13072:SF0">
        <property type="entry name" value="DYNACTIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="182" mass="19031" checksum="A4D691B662C850F1" modified="2022-01-19" version="1">MTARPSLTNRSSTSKLPTPPLSSTPTTLISASASCTGTHLIKIGSKSIVQLRARLTSNYGPISVGDGCIISERASIGLLSSPQNEKEAKGVTLSSGVLIETCAVVQGASIGAYTIIEAGAKIGKGTVVGVEIGEGMVVDDNTVVYGSGGERRKEKKGLGLDRARKVWVDLQEDTLRRMWTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEK0</accession>
    <name>A0A8H6CEK0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151434.1">
        <property type="nucleotide sequence ID" value="XM_037292895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEK0"/>
    <dbReference type="GeneID" id="59330381"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034431524" evidence="1">
        <location>
            <begin position="20"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="182" mass="20282" checksum="31961563198A1AD3" modified="2022-01-19" version="1">MGFLCAAWIVVHFCTVTLAEPPLSPLTLSLLNGLNVNVNLTAPSSLTQLFSSNRLPQDPLLYPVPNSDETVCFYGYGTPLRRRDVLYVFVKATREVAAHGSSNEPIPDRMIRYGSGNAFILLHHSGRMTWKVWEMALQGIADFVERYEYVDMDFDVGKTGSERFFGTGVLGMQKREGINASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFF3</accession>
    <name>A0A8H6CFF3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001471</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151820.1">
        <property type="nucleotide sequence ID" value="XM_037292401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFF3"/>
    <dbReference type="GeneID" id="59329887"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043041">
        <property type="term" value="P:amino acid activation for nonribosomal peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45527:SF1">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45527">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="3">
        <location>
            <begin position="74"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="175" mass="19176" checksum="ADA3F9AD9FDB7FAF" modified="2022-01-19" version="1">MLSKSPHPAGLVFSPPLLTASLIRPVRKRHPSLPQRPKSPVRKKPSAAKAATFAILSSRLPAYNARGTDADLAFGLEESDISNDSNFWTEGGDSVTAMRLITAARERNIALDTETIFNHLVLMDMANSCRPTEPDKDSEVDPRPEELDRNVVQACAEACGVQQDLIEDIFFAANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFP4</accession>
    <name>A0A8H6CFP4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000676</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151975.1">
        <property type="nucleotide sequence ID" value="XM_037291613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFP4"/>
    <dbReference type="GeneID" id="59329094"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="3">
        <location>
            <begin position="277"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="482"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="514"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="535"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="614" mass="67762" checksum="D9E25A7DCBBCB3E1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFT2</accession>
    <name>A0A8H6CFT2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151939.1">
        <property type="nucleotide sequence ID" value="XM_037291577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFT2"/>
    <dbReference type="GeneID" id="59329058"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034068103" evidence="2">
        <location>
            <begin position="24"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="320" mass="34519" checksum="A324219D33073581" modified="2022-01-19" version="1">MRFTLRHALLVAAAGGLFFRASSRPLESELSSLSLERRVDIDPIDPDVSMTDAPATEEASGTQGDDAPEDPTSADPAPAPAAGAASGPKPPLTVPELQARGDTAIENLNNAISQNTPDKNAANFKNVYKVTDVKAMRAAPDLGPILRDPALSGGSKNPRSGTYTEYTVINKVGGDPKNPILDLEVSGDQGTMINEVSYANKDNLPQGQRIHWSDQAFQAWNLEEGNPRLDLVIQRNIRNDDTNNAIQQFHTESKLRNNQPGVWLPGSAQYKALMGTDNGRPVARMLQDHHQAFGNKRVTKITTYATGGSRTKSLMSLQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGL8</accession>
    <name>A0A8H6CGL8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NTF2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000932</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152227.1">
        <property type="nucleotide sequence ID" value="XM_037291865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGL8"/>
    <dbReference type="GeneID" id="59329350"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058645">
        <property type="entry name" value="NTF2-like_dom_7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26534">
        <property type="entry name" value="NTF2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034931002" description="NTF2-like domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="NTF2-like" evidence="2">
        <location>
            <begin position="34"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="139" mass="15199" checksum="A39116F1C11A65D5" modified="2022-01-19" version="1">MKLSYIQTLLLCFLSTLISAHSSPPSTEGSPCGTCIQDNEVEGIAQRWLNAFATGGLPTLDSAVTENIMIYDEGANNGTTSAYVHNRTELYNTISEGPYGGNGVTNVTYDVVFTFHTCDRIALRWQQNEYTTADIGYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGU6</accession>
    <name>A0A8H6CGU6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152347.1">
        <property type="nucleotide sequence ID" value="XM_037291985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGU6"/>
    <dbReference type="GeneID" id="59329470"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035426">
        <property type="entry name" value="Gemin2/Brr1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04938">
        <property type="entry name" value="SIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="514" mass="56346" checksum="53A736C664384B8E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHD6</accession>
    <name>A0A8H6CHD6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_000291</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152665.1">
        <property type="nucleotide sequence ID" value="XM_037291230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHD6"/>
    <dbReference type="GeneID" id="59328710"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF16">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="148"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="8"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6223448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="331" mass="34557" checksum="4B96141730CEB48E" modified="2022-01-19" version="1">MVKAVVLGASGGIGQPLSLLLKASPLVDELSLYDVVNTPGVTADLSHISSIAKITGYLPADDGLKKALTGADVVLIPAGIPRKPGMTRDDLFKINAGIVRDLVKGIGEYSPEAFILVISNPVNSTVPIAAEVMKQAGKFDPKRLFGVTTLDVVRAETFVQDITGEKDPSKTVIPVIGGHSGETIVPLFSLAKPAVKIPAEKLAPLIKRVQFGGDEVVEAKGGAGSATLSMAYAGFRFAEKVIKAAKGEKGIVEPTFIYLPGVTGGDAIAKETGVDYFSVPVELGPSGAEKANNILPQVNDDEKNLLEACKTGLKGSIEKGVEFVHNPPPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHQ5</accession>
    <name>A0A8H6CHQ5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152894.1">
        <property type="nucleotide sequence ID" value="XM_037291459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHQ5"/>
    <dbReference type="GeneID" id="59328939"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="80" mass="8732" checksum="76248B37C6D60F7F" modified="2022-01-19" version="1">MNNDSSSKYSPIRKNAHSPTPYLPSKTPNGDEPSKATNEKRLIAQRFKLDLEAAHADEAASQSLSPLGLVPGYPDSKRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHX1</accession>
    <name>A0A8H6CHX1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable Xaa-Pro aminopeptidase P</fullName>
            <ecNumber evidence="5">3.4.11.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Aminoacylproline aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Prolidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_000399</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.</text>
            <dbReference type="EC" id="3.4.11.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152773.1">
        <property type="nucleotide sequence ID" value="XM_037291338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHX1"/>
    <dbReference type="GeneID" id="59328818"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000010">
        <property type="entry name" value="Probable Xaa-Pro aminopeptidase P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000007">
        <property type="entry name" value="Xaa-Pro aminopeptidase P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="16">
        <location>
            <begin position="266"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24 C-terminal" evidence="17">
        <location>
            <begin position="496"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6223556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="558" mass="62316" checksum="1092242C77DE25F0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI32</accession>
    <name>A0A8H6CI32_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000593</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152967.1">
        <property type="nucleotide sequence ID" value="XM_037291532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI32"/>
    <dbReference type="GeneID" id="59329012"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00625">
        <property type="entry name" value="RCC1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="88"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="5">
        <location>
            <begin position="90"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="145"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="225"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="278"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="332"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="390"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="454"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="507" mass="53145" checksum="95B5F58CE11EF574" modified="2022-01-19" version="1">MAAPKNLSGTKKTATTASKTASRASFASQTAGSENKNTRTSKRKAEELGSSSAVTKSRKFNSSKAASKSTTRAPKPKLILNHRATDTLNVYVFGSGDSGVLGLGPKCSTDDVVRPRLNPNLSTSFVGVVQMATGGMHCVALTNENKILTWGVNDHGALGRDTEWGGGWKDMDGGDHDQDSDNSESELNPKESSPTAIDSSCFPANTVFTQVAAGDNATFALTEEGLVYGWGTFKTSDGALHFSADMAVQNRPTLISGLRNITKIAAGSNHALALDHKGCVFAWGFGEQCQLGRRINARFQQSGLVPATFGLPKDVMDIGAGADHSFAIHRNGTVYGWGANNYCQTGIDERDGEDFANILRPQSIDGLTGHGKVTSISGGNHHSVAVTDGGACLTWGRVDTNALGLSVDSLAEHDIIRDAQKKPRILKNATQVPGINAVHAAAGSDHCVAVTHEGKAYSWGFGVSGQLGLGGTDDVGLATLIENKAVKDKKLVWAGAGGQYSVLTGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI59</accession>
    <name>A0A8H6CI59_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BRO1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010567</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the palA/RIM20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153053.1">
        <property type="nucleotide sequence ID" value="XM_037301419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI59"/>
    <dbReference type="GeneID" id="59338957"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09241">
        <property type="entry name" value="BRO1_ScRim20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09236">
        <property type="entry name" value="V_AnPalA_UmRIM20_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF39">
        <property type="entry name" value="PROGRAMMED CELL DEATH 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="4">
        <location>
            <begin position="4"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="647"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="738"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="796"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="553"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="798"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="833" mass="93587" checksum="D2C4A2D2C3C7A8C3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIA0</accession>
    <name>A0A8H6CIA0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">SUMO-conjugating enzyme UBC9</fullName>
            <ecNumber evidence="4">6.3.5.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Ubiquitin carrier protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Ubiquitin-conjugating enzyme E2-18 kDa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_010642</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153128.1">
        <property type="nucleotide sequence ID" value="XM_037301494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIA0"/>
    <dbReference type="GeneID" id="59339032"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019789">
        <property type="term" value="F:SUMO transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000031">
        <property type="entry name" value="Asparagine synthase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000050">
        <property type="entry name" value="Asparagine synthetase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000035">
        <property type="entry name" value="SUMO-conjugating enzyme ubc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="19">
        <location>
            <begin position="3"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="20">
        <location>
            <begin position="159"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="18">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6224068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="735" mass="83391" checksum="FDF24352832889A1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIU3</accession>
    <name>A0A8H6CIU3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153369.1">
        <property type="nucleotide sequence ID" value="XM_037301735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIU3"/>
    <dbReference type="GeneID" id="59339276"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="479" mass="55643" checksum="E603BD540B7DEA55" modified="2022-01-19" version="1">MTTDTEETFLDIVSGPRHKGFLFLIFSTVTRLESHTTHLRALRNEKLRGSPTPSAMELIAEKIFRKSRTRRDYLWDIMEEKVDMTNVVERAIWVKKKKAVLGDVMGGLVEYLEELGDEEGWKEREVREMGERWEKRWRVYGAMDRADLKKAGWILDVGKRDGRDPSDFNNDGHRTLAVRPRYSERTKSSAGYDSGIRGGVIYCPPTRPGLRQGPMSGSYIGDPFAGLDELPRRAEVYEVPVSYTSDLDGMRSRSSTQTSRPSSRGSEYVYTEYEDDEERERKRFLGTELYGYVMAMRLKDRLLEVRQVLQLYKDFDRASGSIFVIETQRDFIELIRQAIEATREVEKPLRRWAEAGKSKTERGYFTHKLLETASEVIRLWQKRFDWIRTRSDEMLYAHGAKGSSNRPTYTTRREKCLSSVDEASMLLRAREMDEIMEGVQELGDRLKGEWKRDGKLKPDEMMKRKGLKWGTLVGTGRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJN7</accession>
    <name>A0A8H6CJN7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucose-methanol-choline oxidoreductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009968</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153641.1">
        <property type="nucleotide sequence ID" value="XM_037300824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJN7"/>
    <dbReference type="GeneID" id="59338360"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF201">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase C-terminal" evidence="6">
        <location>
            <begin position="243"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="416" mass="45325" checksum="38BF04714C23BA76" modified="2022-01-19" version="1">MTTHIDPFSSAATHFDYLIAGGGTAGLTLAARLTEDPDITVGVFEVGLDRTDDPNVLTPGFTPTILTATIALLTIRVESQLEGSSAISFSSWTHTSQQDIDDWGQLGNPGRFWKELFPYFLKSENYNAPPTSVPEQVDTTFIGSHLHGENSPVQDFFSSFYGEVTFESLRNETFVANALADQILVALNKGPIPQSLLHAPSRSKISGQPKKDALILQKLLDANEEGLQEPGNHFSMLTDLETPFPRGSVHIVSADPTVYPSIDPNYPSHPLDLIVISQAMLHLQQVAGTAPLPTHLKEGGRVFQPGFHELDEQNVKAFVRNSFSSEYHPIGTCAMRPREEGGVVSERMVVHGTRDLSVLDASVFPLQGMRGLRRTLVVLGVAKIGRRRRSGRIIGGWLMFGEGGGGGSDGEVGRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJP7</accession>
    <name>A0A8H6CJP7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009971</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153644.1">
        <property type="nucleotide sequence ID" value="XM_037300827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJP7"/>
    <dbReference type="GeneID" id="59338363"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="234"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="413"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="429"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="504" mass="55885" checksum="E0F41FD7A9B49707" modified="2022-01-19" version="1">MVLGKRGREPEQSDWGAQGRLRSRPKPNPPNVQLPSEQPKQRGKPRRLRLIPPTLQNQTGPSRAESTDPGSSHSETSTCYSQDVTNYDVDRGVEAAGTPQAGDIECWGKQKGAAEFIRSQSGLETSWVGIRPLGKGGCGIAGLWELRGDDGQRYGRFRQHLPEPFLWDTFYHLVEAATAMRNGSKRDKWDFEIVHRDLKPGNVFLGEENEDKGFPFYPVAKTGDFGLAVMTSAKDPSNPSRYRGPGTQGYLAPEQVNDDTPKEEQDKLSSYTNVWAIGATMLELLTLYAHLDYINDPEYNDAGRIRVIETNKEPEYSQDLRDLIKACIEPKPLERIKLEDLRARIKSYHDCIIREYRQSSNDWRARYESDSLLYYARNEINNMPTGEWEPYKAISPSKPEDVKFPDHAWPVVFPRFDDGPEAGGKKGGDGNGDAGKEGGGIKGLKGGLPDGAPDGGTDGSSGDSLSPPPSDPQPPTPFHFPAPQAPAGQPAPRMLRNGRIFGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJS2</accession>
    <name>A0A8H6CJS2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153620.1">
        <property type="nucleotide sequence ID" value="XM_037300803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJS2"/>
    <dbReference type="GeneID" id="59338339"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42095">
        <property type="entry name" value="YALI0C12166P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42095:SF1">
        <property type="entry name" value="YALI0C12166P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="198" mass="20789" checksum="AC448B3CB158BD29" modified="2022-01-19" version="1">MYNNPLLSPITPQQPPPNTQHPTPKPSPPPPPPTTMPTNRPFFFNFLAAFRAQSAISKSSSNPSSSPTTSTSTPTASSTYAPPTLSRAIATKSPSPGATTAAVQAANPSFSHARPASTSPSASMSPPPRSPQAMSPPGGGFRRARRGSDTSSEGGFRDLGEKWYVGGKTAGGEERYLRLGAVRKERSGDRLSLDRLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJZ1</accession>
    <name>A0A8H6CJZ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009996</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153669.1">
        <property type="nucleotide sequence ID" value="XM_037300852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJZ1"/>
    <dbReference type="GeneID" id="59338388"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053187">
        <property type="entry name" value="Notoamide_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47256:SF3">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47256">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="73"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="695"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="696"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="773" mass="86434" checksum="AEE2DDAD840BBCC9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKU2</accession>
    <name>A0A8H6CKU2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154189.1">
        <property type="nucleotide sequence ID" value="XM_037300341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKU2"/>
    <dbReference type="GeneID" id="59337875"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="607" mass="66136" checksum="D41469E7F49142EF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL01</accession>
    <name>A0A8H6CL01_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">beta-glucosidase</fullName>
            <ecNumber evidence="10">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010226</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153898.1">
        <property type="nucleotide sequence ID" value="XM_037301081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL01"/>
    <dbReference type="GeneID" id="59338618"/>
    <dbReference type="UniPathway" id="UPA00696"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000006">
        <property type="entry name" value="Beta-glucosidase H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF27">
        <property type="entry name" value="BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="11">
        <location>
            <begin position="393"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6225031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="829" mass="90704" checksum="FBB03B2B8FF11980" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNS7</accession>
    <name>A0A8H6CNS7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate dehydrogenase substrate binding N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155393.1">
        <property type="nucleotide sequence ID" value="XM_037299392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNS7"/>
    <dbReference type="GeneID" id="59336922"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089:SF1">
        <property type="entry name" value="BIFUNCTIONAL 3-DEHYDROQUINATE DEHYDRATASE_SHIKIMATE DEHYDROGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="SHIKIMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="1">
        <location>
            <begin position="39"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="337" mass="36936" checksum="7F6D3A40767BF512" modified="2022-01-19" version="1">MAAVEPNTLEPNTLLDSYRYGFSALNPQSVASKSFKTYLFGYPIAHSLAPLVQSTLFKELSVPWTYTLFESLSAADFLSKLKAADCIGSAITMPHKVTFVQHVDDLTHEARAIGAINTVFLRRGADGSTRYIGTNTDCIGVREAFLQNIPGIRAQSEGRPALIVGGGGACRAAVYSLWRWMGSSRIYLVNRLESEVHAIIAHFGTVPGFAGDLVHVPTIEAAAELETPMLVVGCVPDLPPKERGETLARDVALTFLRRPEKGHVLEMAYHPNPITAFYLLARQNGWNVLPGTEAMIYQGIAQQSLWMEKPSDEFPVPEAREVIKQALKVTHENTDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPM7</accession>
    <name>A0A8H6CPM7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like-conjugating enzyme ATG10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Autophagy-related protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008736</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155601.1">
        <property type="nucleotide sequence ID" value="XM_037299600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPM7"/>
    <dbReference type="GeneID" id="59337131"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061651">
        <property type="term" value="F:Atg12 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14957">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14957:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6227293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="223" mass="24577" checksum="56675A7FB2805982" modified="2022-01-19" version="1">MSLNRPEEAGFISRKAFDEACQALALCSGLWSSDMSLETRRHGEHQYLKIIRLLSTNVNPPAPSDSAKLFSAAQDSHESSNIEEEDEDVAIYHSPPSLTVSSVEYHILPSTSYRVPVLYFFLHNLPPSILQSIDFVYEKLVPKHLQSGVRNAGVMGGISMTNHPLTDIPCFWVHPCNSAEAMQNILGGIKGEVSPVKYMILWIGIVGSVVGLSLPKEIFSARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPP9</accession>
    <name>A0A8H6CPP9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008763</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155628.1">
        <property type="nucleotide sequence ID" value="XM_037299627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPP9"/>
    <dbReference type="GeneID" id="59337158"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000434">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="39"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="386"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="527"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="713"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="416"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="543"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="614"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="633"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="649"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="730"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="748"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="7">
        <location>
            <begin position="160"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6227320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="793" mass="88121" checksum="4306B42D45C0E4AF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ59</accession>
    <name>A0A8H6CQ59_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Meiotically up-regulated gene 190 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_008648</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155514.1">
        <property type="nucleotide sequence ID" value="XM_037299513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ59"/>
    <dbReference type="GeneID" id="59337043"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04041">
        <property type="entry name" value="C2A_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21676">
        <property type="entry name" value="SMP_Mug190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057349">
        <property type="entry name" value="C2_Mug190_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037767">
        <property type="entry name" value="C2A_Mug190-like"/>
    </dbReference>
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        <property type="entry name" value="C2B_Tricalbin"/>
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        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
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        <property type="entry name" value="MEIOTICALLY UP-REGULATED 190 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47348">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 190 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="C2_Mug190_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="271"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="283"/>
            <end position="305"/>
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    </feature>
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        <location>
            <begin position="337"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="599"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="815"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
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            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="83"/>
            <end position="223"/>
        </location>
    </feature>
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        <location>
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            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="785"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1157"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="450"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1157"/>
            <end position="1173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6227206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1269" mass="142047" checksum="FC1DC041C48B6CCE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRB8</accession>
    <name>A0A8H6CRB8_9LECA</name>
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        <recommendedName>
            <fullName evidence="8">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007865</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037298735.1"/>
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    <dbReference type="GeneID" id="59336262"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF379">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="8">
        <location>
            <begin position="142"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="586"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="835"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="886"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="586"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="622"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="742"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="763"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="795"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="840"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="913" mass="102671" checksum="285679E0546E8F43" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRN1</accession>
    <name>A0A8H6CRN1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 14">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_008028</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10 14">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10 14">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 14">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="10 14"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156232.1">
        <property type="nucleotide sequence ID" value="XM_037298896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRN1"/>
    <dbReference type="GeneID" id="59336425"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="6PGD_dom2"/>
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        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006184">
        <property type="entry name" value="6PGdom_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
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    </dbReference>
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        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK09287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00461">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="5 10 14" id="KW-0521">NADP</keyword>
    <keyword evidence="6 10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10 14" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="201"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="482"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="30"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="151"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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        <location>
            <begin position="208"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>substrate</name>
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        </ligand>
    </feature>
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        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>substrate</name>
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        </ligand>
    </feature>
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        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00002526"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004874"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022857"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00048640"/>
        </source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6228298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="510" mass="56209" checksum="E72163A4EE7F3289" modified="2022-01-19" version="1">MTDKAIARLAGINVGGPAPITPSGDFGLIGLAVMGQNLLLNVADHKEFNVVAFNRTVAKVDRFMDNEAKDYKNIEGAHSMEEFVLKLKKPRRIMLLVMAGKPVDDFITALLPFVEKGDIIIDGGNSHFPDTNRRTRSLAEKGIRFVGTGVSGGEEGARYGPSLMPGGNEEAWPYIKDIFQSISAKSDGEPCCDWVGDQGAGHYVKMVHNGIEYGDMQLICEAYDIMKRGLGLSAKEMADVFAEWNKGVLDSFLIEITRDVLRYDDDDGTPLVEKILDSAGQKGTGKWTAINALDLGMPVTLIGEAVFARCLSSLKQERGRAAGVLAGPTPKFDGDKKKFLADLEQALYASKIISYAQGFMLIQNAAQEYKWKLNKPSIALMWRGGCIIRSVFLKDITSAYRKNPDLENLLFDDFFNKAIHKAQDGWRDIVSKGALWGIPTPAFSTALSWYDGYRTKDLPANLLQAQRDYFGAHTFRIKPDHASEKYPEGKDNHVNWTGRGGDVSASTYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRS4</accession>
    <name>A0A8H6CRS4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription factor TFIIIC triple barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037299073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRS4"/>
    <dbReference type="GeneID" id="59336602"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019481">
        <property type="entry name" value="TFIIIC_triple_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10419">
        <property type="entry name" value="TFIIIC_sub6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor TFIIIC triple barrel" evidence="2">
        <location>
            <begin position="22"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="458"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="485" mass="51524" checksum="E31E4BF734D6C054" modified="2022-01-19" version="1">MAGIHFAAEEDDSDWDYEYHETETESFYVTLDISSAADYNKLRTRKRKPETSPPKSPQPATSIAPTTPVAQDNAGPSEPPEIPIPETPAPMDIDPTLGQPKEYPSHFEPKDRIQILDLHTQNPLISYQNQMYSCDWTSTLGTDLLLTAPMPDSSHPVLREKPNVSVLATSSIKLMGRPAQIASRHSAEQGGQSSTPAPESSIASANTASSEKATPVKISLGATPSRARQNQANFLERLIAIKARKGEKDSVTIHAQQVNQGTGWRSQRKASEAIGDGNDEITPTQSQRGRDTGGRPRGSKRTRGPRTAKGGLFRDYRPQLWDTPGADIRAGPSSTPDNWDQLEGGASDGFQTAASRIADASPLPADQPAQAANRSTRSLSASASANASPIPSFRPVQSIHGDPSSSYALAAASPSLALALGLQPAGLMEKSVIPASGVPDTEPLQQNQAQDAIAEQGTPAPETATAALETSGMAAADDVEMTDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS59</accession>
    <name>A0A8H6CS59_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156542.1">
        <property type="nucleotide sequence ID" value="XM_037297907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS59"/>
    <dbReference type="GeneID" id="59335412"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="114" mass="13113" checksum="F2F50FEE6E8CFE19" modified="2022-01-19" version="1">MEKPERLPFREKSVKDPGKGVDHRKKKERHSAKVEEDKLQEELEEETELFRACDDKTIELNGLKKFSKNFKFDRPISEDLSPVLAKDGTRQLEATSKASGENGQKNEQREPKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSS5</accession>
    <name>A0A8H6CSS5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein arginine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_006968</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156499.1">
        <property type="nucleotide sequence ID" value="XM_037297864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSS5"/>
    <dbReference type="GeneID" id="59335368"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007857">
        <property type="entry name" value="Arg_MeTrfase_PRMT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035075">
        <property type="entry name" value="PRMT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035248">
        <property type="entry name" value="PRMT5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035247">
        <property type="entry name" value="PRMT5_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738:SF0">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05185">
        <property type="entry name" value="PRMT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17286">
        <property type="entry name" value="PRMT5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17285">
        <property type="entry name" value="PRMT5_TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015894">
        <property type="entry name" value="Skb1_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRMT5 TIM barrel" evidence="11">
        <location>
            <begin position="36"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 arginine-N-methyltransferase" evidence="10">
        <location>
            <begin position="407"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 oligomerisation" evidence="12">
        <location>
            <begin position="625"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="201"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="338"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="343"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="592"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="601"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="442"/>
            <end position="443"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="531"/>
            <end position="532"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for specifying symmetric addition of methyl groups" evidence="7">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR015894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6228857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="840" mass="92701" checksum="37D049A3C7EC1C5E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F704</accession>
    <name>A0A8H6F704_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006604</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147212.1">
        <property type="nucleotide sequence ID" value="XM_037297502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F704"/>
    <dbReference type="GeneID" id="59335005"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034742072" evidence="2">
        <location>
            <begin position="22"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="159" mass="15238" checksum="652CB2E7E44F028E" modified="2022-01-19" version="1">MRYSIILPAFAAAALAQSANGKSYTETNSLGVVTAQPGVVTTQPAAVTTQPAVATAQSAAAVVPAGANSTIVAVPPGSTGNGSSGTNGTVNSASLKSASTKTSSTSHSSSDTSSTSTSHSGSSTTSSGPSTTSKKASAADIVQPILGLGFAGLIFAAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7V4</accession>
    <name>A0A8H6F7V4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrapyrrole biosynthesis uroporphyrinogen III synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147885.1">
        <property type="nucleotide sequence ID" value="XM_037296704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7V4"/>
    <dbReference type="GeneID" id="59334204"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390">
        <property type="entry name" value="UROPORPHYRINOGEN III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390:SF0">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="1">
        <location>
            <begin position="78"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="327" mass="35566" checksum="95B1CB4B45A6479A" modified="2022-01-19" version="1">MSTTANSPTPPIPILLLKSPSPTPASDAYTLHLTPPFRPTFVPVLSHTLLPDPLIKLLLTHFTPNPSPGHLPDPNPFPYGALIFTSQRSVAAFASALNAPQVVKIREEVADGLGGGGVRLYVVGPATGRALRAVRDERCAGCGIHGGEEAGSGEVLARIMLGEGEGKEAYSLREREGGSMKPALFLVGEKRRDIIPRMLMDPKLPEGERIQVDEMEVYKTGELEEFEFHFTQTLTRTEPETVRWVVVFSPTAGEGMLRGLGWLDKASERVKACIGDPGRRTFVACIGPTTRNYLVQEFGFEPDVIAQKPSPQGVKEGIESFMKERAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F878</accession>
    <name>A0A8H6F878_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative gamma-glutamylcyclotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005430</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148322.1">
        <property type="nucleotide sequence ID" value="XM_037296340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F878"/>
    <dbReference type="GeneID" id="59333836"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045038">
        <property type="entry name" value="AIG2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31544">
        <property type="entry name" value="AIG2-LIKE PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31544:SF2">
        <property type="entry name" value="AIG2-LIKE PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="4">
        <location>
            <begin position="25"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="195" mass="22365" checksum="39E347B8F7A387E0" modified="2022-01-19" version="1">MAPQVLYRVVYSTTNPTPSQINRLVIEPATLPQYTRRRVRDCDYPAIVPSSEPGACVRGTYVRGLTRDDQWRLDLFEGSQYERIRLRLHLRDRKRNEGEEMEAETYVWAEDTRGLEDGEWDFEEFSREKMGRWIGNDEEYEGELRPSIAANVITVEVDEAVRKKDPTGGRGVNGIIGGKLEVKGKSEKEVLESAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8C5</accession>
    <name>A0A8H6F8C5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005736</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147824.1">
        <property type="nucleotide sequence ID" value="XM_037296643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8C5"/>
    <dbReference type="GeneID" id="59334141"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="68" mass="7640" checksum="6E59BA7E2A11D03F" modified="2022-01-19" version="1">MAGLRTVALDEADEPQRLNLKDDNQWASGSSIPGLNALILQPSLREYNLLLRYTRPEQKTLTLENSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8K3</accession>
    <name>A0A8H6F8K3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005087</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148697.1">
        <property type="nucleotide sequence ID" value="XM_037295999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8K3"/>
    <dbReference type="GeneID" id="59333493"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052355">
        <property type="entry name" value="CENP-V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620:SF1">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620">
        <property type="entry name" value="CENTROMERE PROTEIN V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="4">
        <location>
            <begin position="242"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="365" mass="40375" checksum="1BEF1E42CDF10A50" modified="2022-01-19" version="1">MRIFLLGGSGRTGSLTLAEALSRNHTVTALVRRPDALPSQPNVSIITGTPLNQADISNSFASAPRSDPIRVVISTLNTGRTSDNPWAKTTSPSNLMAESVRNTLAVMKEHGVKKMVVLGTNGVGSSRANGGWLFNWIVDHSNLKVTFDDHYEVQKLLEAEAEKDVDFKWVDVRATGLDNGEKKEVKEFGNEGKGVGWFISRKSVAGFLLDAVEGSQWDRQTPLYYMEWQLTVCAIMNTEVLHKGSCHCKKVNLEVLHSPDIELSECNCSICSMKGFLGFVIEKSALKSLQGEENLEVYKFGTGTAQHMFCKCCGISPLTVPRSYPEGYNINYRCLARDNVRSINVTPRDGQNWEQEMAKDIVGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9E1</accession>
    <name>A0A8H6F9E1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003671</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149281.1">
        <property type="nucleotide sequence ID" value="XM_037294593.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332082"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057962">
        <property type="entry name" value="SPT23_MGA2_DBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25603">
        <property type="entry name" value="SPT23_MGA2_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1303"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="6">
        <location>
            <begin position="755"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="941"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="974"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="70"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="185"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="367"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="589"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1016"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1055"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="604"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="647"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1016"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1075"/>
            <end position="1084"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1350" mass="146312" checksum="A7DFDC788D5618F7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9K7</accession>
    <name>A0A8H6F9K7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 34 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003382</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149685.1">
        <property type="nucleotide sequence ID" value="XM_037294304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9K7"/>
    <dbReference type="GeneID" id="59331793"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000117">
        <property type="entry name" value="Glycosyltransferase family 34 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF5">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="54634" checksum="00B0F35E56337217" modified="2022-01-19" version="1">MSLSRSPSPRRGGGWSSPGLTSPYDNVSGNASPRKTYGDTQTNGSSSGDNVTWAKAKARSEEVKGYPSFSTRNNGFFSRHARKISTSLPTFRMTGRRDFSDKERLGRGRWAQMGGSRTGRFLTYLGRTAWRLRLRLGIFLAFVLAIILFYVTPMHRAYRRASFLGGGSKFVVILAANQGGGVMEWKGAREWAIERDSVRNKKKYTKKWGYDLEIVDMSTKKRYAHEWRESWEKVDTIRNCMRKYPKAEWFWWLDLNTFIMEPSYPLQKHIFNDLLGNTYRDINVYNPLNISHPFQSPHLDLPARSANGDGHANSIDLIIPQDCGGFNLGSFFIHRSVWTDRLLDIWWDPVGYEQKHMEWEHKEQDAMEHLYINQPWIRPHTAFIPQRKINSFPMGACGENGEDSRIHYSERDRDFVVNMAGCEWGRDCWGEMYNYRELSNKLNRTPWEKFKDGISDRVKTLRGKKEEGTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA50</accession>
    <name>A0A8H6FA50_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150285.1">
        <property type="nucleotide sequence ID" value="XM_037293456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA50"/>
    <dbReference type="GeneID" id="59330943"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="233" mass="26933" checksum="366EEDF7322D0563" modified="2022-01-19" version="1">MTAQSVGHAELKTRARERDEDNLKLKAENEALGQKRDRDAQITAAVNRDWTAVQPHLDNVLRETNVVLVQQRDVLMKDLDQAHRKNDDLQKLTQGLMEKQEDSESSNVAKLEKEQQELVQLRVRVSALGKAAEQADIQRKRLEHVRRAENRGRELKFEPGNDKLRAKAKALELTCNDMKPKIAKLQAVVQRARRTREAFVHRWDEIKRLTYSTHKEQQLLAALAEIVNEELLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA91</accession>
    <name>A0A8H6FA91_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150136.1">
        <property type="nucleotide sequence ID" value="XM_037294058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA91"/>
    <dbReference type="GeneID" id="59331546"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="279"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="365"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="400" mass="44027" checksum="CFECF64BBF0A4FD1" modified="2022-01-19" version="1">MSSVQALANIDTSLPALEKTAQILQDQSKRFGEQQRLKGKQGDKQVVEQKDTAGDLEAQASADTGELRKEVSKGQRAEADAFTIQSLRERIIHLEEEEKTRLEAEKEIVCESIQLWSIEHEMRLSLESTANVDAAHLIRNQNGLLKDAKANIHALECELQAYKDRYGSLGRPLFRGPSWGPSNWLQHTPPSTPSSLHLHQHQFRHIRPKVRLPTIGAGQGTSFVTTPGQSQVVSESPAPTPHLGGGPLQPSSSFVPTLSHPRVVNEAPASADRLGQNVFHPHSSIVPSPSQSPVVDKTATSTAPSGHGVFEPNLPRNPVSQRLRMQDQSQRRATVLPHLRHLQKAPQPAGTTHIQPGLPRPQPDTPSGSRDTQDTESSVQTQVNHTFYNWIDGMWQAEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAA4</accession>
    <name>A0A8H6FAA4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150557.1">
        <property type="nucleotide sequence ID" value="XM_037293728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAA4"/>
    <dbReference type="GeneID" id="59331215"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="161" mass="17705" checksum="BCDB132C0D86BAE6" modified="2022-01-19" version="1">MPDEPADPVLRDNEPITPAVKTTGPPPVNNPSLPIVNDALDILRGPLLNYKGMQIVESEVPAFWHGNVLIVANPLECLGIRSQMGQSGCSLPNDIDEPENVRKGSTSNPPEVRHASGNSVVKHYSDSVKEFWRFAVDFALKDHKARWQYALTNERPSGSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAC6</accession>
    <name>A0A8H6FAC6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 11">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_002036</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 11"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150617.1">
        <property type="nucleotide sequence ID" value="XM_037292964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAC6"/>
    <dbReference type="GeneID" id="59330450"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.960:FF:000002">
        <property type="entry name" value="Glutamate-cysteine ligase Gcs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000001">
        <property type="entry name" value="Glutamate-cysteine ligase Gcs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000004">
        <property type="entry name" value="Glutamate-cysteine ligase Gcs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="590"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="599"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6221182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="748" mass="85470" checksum="53A7A20011A98FD8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAD3</accession>
    <name>A0A8H6FAD3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_002486</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150241.1">
        <property type="nucleotide sequence ID" value="XM_037293412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAD3"/>
    <dbReference type="GeneID" id="59330899"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF154">
        <property type="entry name" value="PAP2 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G09730)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="110"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="396" mass="43303" checksum="29CFF1894F6E17F3" modified="2022-01-19" version="1">MASLTHTPKRLALSYVVDWIVIIGIAAVGAGFWKITPNHRPFSPVDPDISFPYVEHEKISTGLLVTIALVIPALVTAFVTLVFTPGPGVSRGTPKSLMWRMKLWEWNTAWMGLALALATTFFFTEGLKNVIGRPRPDLLSRCNLDPATVQQYALGGEGSQLPLWNLLVSSTACRQPNASKLNDGFASFPSGHSSCKSVLCLLSLGWLTVLCLVSWAGMFYLTLFLCSKFSVTIPYLLPYTFESSTQDAKRYDQGLIDGDSDNFKNDAPASTKSRSVPLRKQAAAPPTYLLILPLICISIPAYVASTRFSDFRHHGFDIIFGSLMGTVISYIGFRMYHLPIRRGAGWSWGPRSVSRAWGIGVGPQGYTDGNGAMTKKRDLEAANEPRNSALVSSGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAN9</accession>
    <name>A0A8H6FAN9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_002291</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150870.1">
        <property type="nucleotide sequence ID" value="XM_037293217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAN9"/>
    <dbReference type="GeneID" id="59330704"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="305"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="556"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="560"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="576"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6221435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="682" mass="74541" checksum="A0D2B78DEFCC072F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAU2</accession>
    <name>A0A8H6FAU2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_002156</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150736.1">
        <property type="nucleotide sequence ID" value="XM_037293083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAU2"/>
    <dbReference type="GeneID" id="59330570"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF6">
        <property type="entry name" value="MANNAN POLYMERASE COMPLEXES SUBUNIT MNN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="375" mass="41517" checksum="3EBB4299D1A9BBF4" modified="2022-01-19" version="1">MALPRSIRRTSPITLILAAVLSFGFLVFLFAPSSNSGAVSTAKRKIANAAAHPLSPPTAPFVKPDASLNGLSKPPPVVHYHLNNVTTTADPIGNQETVLILTPLARFYQGYWNNLLALSYPHSLIALGFIIPKTKEGHTATAALQAAIATTQQGPEGKRFASVTILRQDFDPPISSQDESERHKKENQKARRAAMSRARNSLLFTTLGPSTSWVLWLDADIVETPPTLIQDLAFHDKPVLVPNCFQRFTNEENKPDTRPYDYNSWQDSQVAADMARDMGPEDIIFEGYADIATYRTLMAMTADRSETRNQQAIIPLDGVGGTALLVKAEVHRDGAMFPPFPFYHLIETEGFAKMARRLGWGCFGLPNYFVYHYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAX8</accession>
    <name>A0A8H6FAX8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AhpC/TSA antioxidant enzyme-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150613.1">
        <property type="nucleotide sequence ID" value="XM_037292960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAX8"/>
    <dbReference type="GeneID" id="59330446"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02970">
        <property type="entry name" value="PRX_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000404">
        <property type="entry name" value="WGS project CABT00000000 data, contig 2.14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032801">
        <property type="entry name" value="PXL2A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630:SF3">
        <property type="entry name" value="PEROXIREDOXIN-LIKE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13911">
        <property type="entry name" value="AhpC-TSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="36697" checksum="D7739AFACDBAB05C" modified="2022-01-19" version="1">MPGKDPSADFKGNIKVNNNPPTKEDLEKIADLPVLDKDGKKCTFKSLYADNENGPRRVLIIFIRHFFCGNCQEYLRTFASSITPESLSSLSPPTQIVVIGCGQPHLIPMYIQETGCTFPIYADPTRKLYHLLRMTSTLTLGKTPEYMQRSILSMAVRSFIQELKSGRNMLSGGDFRQVGGEFFFQNGKVTWCRRMRSTRDHAEIPVVRQQLGLDDTPAPRRKRWSAAGIGDGITRRLSNRRPMSWAGGPRSKSGNRLERPASGMDFLREEDGEKLINGNGVASGHDVGANGAATNRVATNGATTNGTANGATVNGSGNGGTTNGRAILERINFDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB67</accession>
    <name>A0A8H6FB67_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001644</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151111.1">
        <property type="nucleotide sequence ID" value="XM_037292572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB67"/>
    <dbReference type="GeneID" id="59330058"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="28"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="422"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="630" mass="71573" checksum="3CF97AEBA0D948BE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB73</accession>
    <name>A0A8H6FB73_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002334</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150913.1">
        <property type="nucleotide sequence ID" value="XM_037293260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB73"/>
    <dbReference type="GeneID" id="59330747"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="426"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="463"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="550"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="580"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="640"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="787"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="857" mass="97405" checksum="9AC4122819CC8ED6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBS1</accession>
    <name>A0A8H6FBS1_9LECA</name>
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        <recommendedName>
            <fullName evidence="9">Catalase</fullName>
            <ecNumber evidence="9">1.11.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_001134</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the catalase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.6" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000012">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037292064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBS1"/>
    <dbReference type="GeneID" id="59329550"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="catalase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF62">
        <property type="entry name" value="CATALASE T"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7 9" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="37"/>
            <end position="425"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="576"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6222048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="592" mass="66166" checksum="C90207D30FF90F22" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC17</accession>
    <name>A0A8H6FC17_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151768.1">
        <property type="nucleotide sequence ID" value="XM_037292349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC17"/>
    <dbReference type="GeneID" id="59329835"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="166" mass="18457" checksum="1C32162DCEF3EEB8" modified="2022-01-19" version="1">MSISQHPQEGWFENSPTPNSPNETQRARQRIPNPPTPSIRAHTKSPTAVQTPINPASESALPAFATLHVENGYFERVKGRAGESHRPRSLNRRSKKARERPGSQAADFASLDTQLWNDAVGKDIRTAQATVNETRGDERSNPTRMYVKQRKDIADDHPAQSGIAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCG7</accession>
    <name>A0A8H6FCG7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152174.1">
        <property type="nucleotide sequence ID" value="XM_037291812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCG7"/>
    <dbReference type="GeneID" id="59329297"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="78" mass="8723" checksum="B4FDB390E737B403" modified="2022-01-19" version="1">MPNQASRRGISELRIDIIRFIIEGVEIIDLSYARNNTLYNALSLYGNTSALKSSIQRDDEHQTRSGFSIGVVGLPKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCI4</accession>
    <name>A0A8H6FCI4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152527.1">
        <property type="nucleotide sequence ID" value="XM_037291092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCI4"/>
    <dbReference type="GeneID" id="59328571"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="235" mass="25355" checksum="EF73232B647B69DF" modified="2022-01-19" version="1">MSFGFGVGDFLAASNIAKGVYEACKDGPEEYQRDIERDDGARAQQRFVSVASTTSLLSQIETHEDDVWMIFKTELLAEDVSMAHIMSNKEEIIEHIKSLVVGGLPNGGYEVTPGQIYGSIANSSASSASEPLAPNRGHIRSPDNCGKSFGSQSSPSGSADVQLVLGTSENAQLTATSIASSLDNIQLAWSRIKKWSQAYESDGGVDDDGFVERLRQLLKALEMIIKTLGNTLEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD40</accession>
    <name>A0A8H6FD40_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000286</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0357 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152660.1">
        <property type="nucleotide sequence ID" value="XM_037291225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD40"/>
    <dbReference type="GeneID" id="59328705"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018559">
        <property type="entry name" value="DUF2015"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28023">
        <property type="entry name" value="UPF0357 PROTEIN YCL012C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28023:SF1">
        <property type="entry name" value="UPF0357 PROTEIN YCL012C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09435">
        <property type="entry name" value="DUF2015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="123" mass="13998" checksum="3AA37757BA0CAA50" modified="2022-01-19" version="1">MGYILYSLTFSSFVLATALYLTRNRWNHLVPIPDYIYTRLPSSFASDIESGFTSTDFDLSTNVAAGDSRAGLDRQAKREVQKIMKSRRIGFDEARRMYIEARFAKNNIGPDGLPRDPKFVSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDD3</accession>
    <name>A0A8H6FDD3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010939</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153422.1">
        <property type="nucleotide sequence ID" value="XM_037301788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDD3"/>
    <dbReference type="GeneID" id="59339329"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46972:SF1">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46972">
        <property type="entry name" value="MONOOXYGENASE ASQM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="14"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="91"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="137" mass="15166" checksum="B611FCBD7272D7D5" modified="2022-01-19" version="1">MLPVGSKWSPRPGVTLLGDAARLMTPFAGVGVNAALADALDLSRVLLKRKVNFEADLPDNLADALQEYEGPMFERARENMEKTWGGLQHHFSANGVDDRGRAKQREAAKLGEEAELREKAKRWEQGTLPSLSLQQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDU7</accession>
    <name>A0A8H6FDU7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-CoA synthetase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010799</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153284.1">
        <property type="nucleotide sequence ID" value="XM_037301650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDU7"/>
    <dbReference type="GeneID" id="59339189"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF422">
        <property type="entry name" value="BCDNA.GH02901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="46"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="2">
        <location>
            <begin position="433"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="58491" checksum="145A4F3F5A2A3BBE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDX4</accession>
    <name>A0A8H6FDX4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_010111</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153784.1">
        <property type="nucleotide sequence ID" value="XM_037300967.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338503"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028942">
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    </dbReference>
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        <property type="entry name" value="WSTF_Acf1_Cbp146"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10537">
        <property type="entry name" value="WAC_Acf1_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="118"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
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    <feature type="domain" description="WAC" evidence="17">
        <location>
            <begin position="344"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="DDT" evidence="16">
        <location>
            <begin position="714"/>
            <end position="777"/>
        </location>
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        <location>
            <begin position="789"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="983"/>
            <end position="1075"/>
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    </feature>
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        <location>
            <begin position="1251"/>
            <end position="1271"/>
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    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1320"/>
            <end position="1346"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1011"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1038"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
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            <begin position="1260"/>
            <end position="1271"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1327"/>
            <end position="1346"/>
        </location>
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        <source>
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        <source>
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        </source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1346" mass="155284" checksum="A63BF0B0F54BA018" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEQ8</accession>
    <name>A0A8H6FEQ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010320</name>
    </gene>
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        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="348" mass="38735" checksum="7A029439D4DD1FC1" modified="2022-01-19" version="1">MSETLKFLYRQFFLTPPKANVEKSNLRGQTGIVTGSNIGLGFEASRQLLELGLSHLILAVRSKEKGNNARIELAREAPGAKIEVWDLNMSNYESISQFTQRCATLPRLDFAILNAGIFKPELEINKVTGHEEVVQVNYLSTALLAIQLLPVMDKKNSTRSRPGTITIVGSETAEWAMFKEQKQDPIFPAFNNPKYFDMQDRYYTSKLLQEILLIEMCRTLPSSRGIVNVVNPGFCYGSGLHAEVTTRYRALGIVFTAIKRMIGRSTSVGARTLVDAAVAQGPASHGQYLSDCARYHFPPQLSGQKGDALSKRVWNETLKELSFANVEGILGSMKEKEMTGKSKGDYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FET8</accession>
    <name>A0A8H6FET8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010440</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154110.1">
        <property type="nucleotide sequence ID" value="XM_037301293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FET8"/>
    <dbReference type="GeneID" id="59338831"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF25">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14390)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="9">
        <location>
            <begin position="12"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6225243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="380" mass="40713" checksum="077B84B7C37C9854" modified="2022-01-19" version="1">MGKLCRASVLYGPKDLRLEERGLESPDPTELQIAIRATGLCGSDLHYYQHYRNGDIQVTEPLSLGHESAGVVVVVGSQVDDFKVGDRVALEVGLPCGECERCREGRYNICQHLRFRSSAKASPHFQGTLQDRINHPASWCHKLPDHVSYDVGAILEPLGVAIQASRRAQIAQESSTLVLGAGAVGLLCAAMAKVVGSSHVVIADIQQERVKFATQNGFAHEGFTVPAKRGQNIDEKLQIAKETAASATEKIRNGTGSTGGFDVVLECTGVEACTQAAIYATRPAGKVVIVGMGNPVQTLPISAAALREVDILGTFRYANTYPQAIELVSGHNPLLADLGKLVTHRFLGLDHVERAFEMAARANDADGNLVLKVVVESDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF58</accession>
    <name>A0A8H6FF58_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154312.1">
        <property type="nucleotide sequence ID" value="XM_037300464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF58"/>
    <dbReference type="GeneID" id="59337998"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="1">
        <location>
            <begin position="75"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="235" mass="26398" checksum="C02AD2F86773375C" modified="2022-01-19" version="1">MDFKDAKLAQFRLNNSKIDIYAAQLAKVIHGGVAAETHYWAGSRNVLAVVYDPEAAGVTYIETGFFIGMSPEQASKQLRMLRDFARFYAACWLHPQLIMQNLRENIRATVSNKHTLLESTLPPRFTAIVSCALRELGELFHPHYPQVLTHGDLCPVNLLVSPSTGRVTGIIEWAEVELLPFGLALYGLDVLLGYLGLGGWSYLEIRDELEQNFGNISRTASLMLRGHQKTEYEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG07</accession>
    <name>A0A8H6FG07_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009355</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155042.1">
        <property type="nucleotide sequence ID" value="XM_037300216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG07"/>
    <dbReference type="GeneID" id="59337750"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd12910">
        <property type="entry name" value="SPRY_SSH4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035780">
        <property type="entry name" value="SPRY_Ssh4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SPRY" evidence="6">
        <location>
            <begin position="211"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="373" mass="40889" checksum="1E9A423BF86688B2" modified="2022-01-19" version="1">MGFFKPLKGDHSTAHEEAYPSHPGPPPVREPTYQAPPGPPPSHQFHNSSAHGPSPGGNEYAPPPGPPPGWQKTPSQTPSAPPNTDEEPPPYHDWTVVPDNSLLPPPPVLGHRKSPSSNANLSDADRAHEWCKINPMIRPHQPTHLQHVAVLKGDVRLLKPPEYKGDLLMPNTGFWKGSTRAGSKDSCLLTSSPIYFAYADSPLRTRNAKTIYFEVKVRSLGLGRGADESSIALGYCAIPYPTWRLPGWERGSLAVHGDDGRRYINNTWGGKDFTSPIKTGDTVGLGMTFAVAASVPGYEAQPKCEPTVKVEVFLTRNGQRNESWDLHEELDKDQDLGVDGLDGQLDLYCAVGTFGGVDFDVSFNNRNWLWQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG53</accession>
    <name>A0A8H6FG53_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008745</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155610.1">
        <property type="nucleotide sequence ID" value="XM_037299609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG53"/>
    <dbReference type="GeneID" id="59337140"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000100">
        <property type="entry name" value="Sensor histidine kinase/response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF2">
        <property type="entry name" value="HISTIDINE KINASE M7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="527"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="753"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="376"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="642"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="830"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="711"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="830"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6227302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="905" mass="100732" checksum="D4EC917F6178A28E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG81</accession>
    <name>A0A8H6FG81_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008836</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155700.1">
        <property type="nucleotide sequence ID" value="XM_037299699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG81"/>
    <dbReference type="GeneID" id="59337231"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054289">
        <property type="entry name" value="DUF7025"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411:SF2">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22942">
        <property type="entry name" value="DUF7025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="532"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="685" mass="79013" checksum="D7AB22FE9EDFEED7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGB8</accession>
    <name>A0A8H6FGB8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8004 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155384.1">
        <property type="nucleotide sequence ID" value="XM_037299383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGB8"/>
    <dbReference type="GeneID" id="59336913"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058317">
        <property type="entry name" value="DUF8004"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39601">
        <property type="entry name" value="CHORIOGENIN HMINOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39601:SF2">
        <property type="entry name" value="CHORIOGENIN HMINOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26013">
        <property type="entry name" value="DUF8004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8004" evidence="2">
        <location>
            <begin position="396"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="772"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="800"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="838"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="880" mass="99695" checksum="D2528618A8E078D9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH54</accession>
    <name>A0A8H6FH54_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156331.1">
        <property type="nucleotide sequence ID" value="XM_037298995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH54"/>
    <dbReference type="GeneID" id="59336524"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="629" mass="69065" checksum="380C90D1CBE80FFE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHJ8</accession>
    <name>A0A8H6FHJ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="3 6">2.1.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007777</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PanB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="3 6"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155983.1">
        <property type="nucleotide sequence ID" value="XM_037298647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHJ8"/>
    <dbReference type="GeneID" id="59336174"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00003"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000003">
        <property type="entry name" value="3-methyl-2-oxobutanoate hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001452">
        <property type="entry name" value="PRK00311.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881:SF0">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="338" mass="36284" checksum="1BAA9F9AEA993C00" modified="2022-01-19" version="1">MAQRIMRPHTLQKTLKSTFLPVTVAPTRPGAISLCQTRRSSHSPLGAPPAAPRKKVTINTLQALYKKDEPITMMTAHDFPSAHVADHAGMDIILVGDSLAMVALGMEDTSEVVLEEMILHCRSVARATKAAFTIGDLPMGSYEIAPEQALESAIRVVKEGRVQGIKLEGGLEMAPTIERITRAGIPVLGHIGLTPQRQNALGGFRVQGKTTLGALKVLEDAEAVQAAGCFAVVLEAVPAEVASLVTKKLKIPTIGIGAGNGCSGQVLVQVDMSGNFPPGRFLPKFVKQYGDVWGESMKAIQAYKTDVKSRAYPAPEHTYPMPAKEFAEFENLVKSRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHQ0</accession>
    <name>A0A8H6FHQ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007974</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156178.1">
        <property type="nucleotide sequence ID" value="XM_037298842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHQ0"/>
    <dbReference type="GeneID" id="59336371"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF1">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="33446" checksum="83C9FAFA86225C5B" modified="2022-01-19" version="1">MTALQVPQPDKERPSKDVAWYNPDVESNIRPEMRDLLLNYSKIPAEDLVGHITKVRDAAWDICPYPTVGLLLFLDFGIKCTDLPEPRAQLHTSILHTLMQGGKLLDVGCMFGQDLRKMVYEGAPVSSVYGTDLRGDYFPLGYALFKDETIIPQDHFIAADIMDQASPLCRFDGDLDVISTKDVFHCFDYATQIELAMRFVALLKPRPGSRAVGQHVGHVSAGVYPTAFKDAGLANMFLHNDESFARMWEEVGKKTGSRWRVESRMWKIKPHWEPVQVPGQPRGPDNRTLLFAVERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHV4</accession>
    <name>A0A8H6FHV4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Fructose-bisphosphate aldolase</fullName>
            <shortName evidence="13">FBP aldolase</shortName>
            <ecNumber evidence="5 13">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_007017</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12 13">Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the class II fructose-bisphosphate aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="5 13"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156547.1">
        <property type="nucleotide sequence ID" value="XM_037297912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHV4"/>
    <dbReference type="GeneID" id="59335417"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00946">
        <property type="entry name" value="FBP_aldolase_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000013">
        <property type="entry name" value="Class II fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000771">
        <property type="entry name" value="FBA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006411">
        <property type="entry name" value="Fruct_bisP_bact"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00167">
        <property type="entry name" value="cbbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01520">
        <property type="entry name" value="FruBisAldo_II_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006628">
        <property type="entry name" value="PRK09197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30559:SF0">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30559">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01116">
        <property type="entry name" value="F_bP_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001359">
        <property type="entry name" value="F_bP_aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00602">
        <property type="entry name" value="ALDOLASE_CLASS_II_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00806">
        <property type="entry name" value="ALDOLASE_CLASS_II_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12 13" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="267"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="288"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6228905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="360" mass="40208" checksum="88DA805DCB98AE05" modified="2022-01-19" version="1">MGVEEVLERKTGVIVGDDVYNLFKYAQKHEFAIPAINVTSSSTVVAALEAARDEKSPIILQMSQGGAAYFCGKGVDNKAQKASIMGAVAGAHYIRSVAPAYDIPVILHTDHCAKKLLPWLDGMLEHDEKHFKAHGEPLFSSHMIDLSEEPVDWNIETTAKYLKRAAPMKQFLEMEIGITGGEEDGVNNEDVDNNSLYTQPEDIHNIYKTLSAISPYFSIAAGFGNVHGVYKPGNVRLHPELLKKHQTYVKEKETTKDDKPVFLVFHGGSGSSKEEYKEAISYGVVKVNLDTDLQYAYLTGIRDYVLKHKDYLLHQIGNPEGADKPNKKYYDPRIWVREGEKTMTKRVEEGLKDFNTAGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI93</accession>
    <name>A0A8H6FI93_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156653.1">
        <property type="nucleotide sequence ID" value="XM_037298018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI93"/>
    <dbReference type="GeneID" id="59335525"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052228">
        <property type="entry name" value="Sec_Metab_Biosynth_Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47534:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE-LIKE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47534">
        <property type="entry name" value="YALI0E05731P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="339" mass="36346" checksum="27A109C7DE5CE1F8" modified="2022-01-19" version="1">MVSLIDVQSSNSRIASALPPRLVAVFVGATSGVGEVSLKQFAKHARQPRVYFVGRSQEAGDRIAAECKTLNSEGEYIFIKADTSLIRTVDDICSDIKCKEKAVNLLFLSTGSLVSAETSEGLNFVTALVTYSRNRFIVNLLPLLQQATALRRVVTAFAAGKEGPVSINDFQGWKVSALSARGHVSSLVTVSLEALAKKAPDVTFIHNFPGPLKSNLFRGGQGATIFVINIVFKAISPMMTWYSNQECGERHLFLATSARYPAASHGNITSGVPLSGEVAIARGTNGESGSGVYSVDLAGESSGPKVEELLTNFKKGGVGEKFWRHTEEEFERITGRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIA8</accession>
    <name>A0A8H6FIA8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450 alkane hydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007188</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156716.1">
        <property type="nucleotide sequence ID" value="XM_037298081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIA8"/>
    <dbReference type="GeneID" id="59335588"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF17">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6229074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="22563" checksum="8CC501C1D00FC233" modified="2022-01-19" version="1">MAASETTAFLISNAFFQLSRNPPVLQRLRDEVNSLGPSKAVELDFERLSSMKYLRDVLNETLRLHPIFSQMFRVALRDTKLPLGGGPNGTSPVYVREGTVLATSFYTLHRLPSIWGSDAADFKPERWYTFKPNPWEYVPFGGGSRACVGQQKALMEASYVIVRVLQEYSHIESRDGREWEGHVQMTSKSANGCKLSLTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIF8</accession>
    <name>A0A8H6FIF8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7708 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156743.1">
        <property type="nucleotide sequence ID" value="XM_037298108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIF8"/>
    <dbReference type="GeneID" id="59335615"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056125">
        <property type="entry name" value="DUF7708"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35205">
        <property type="entry name" value="NB-ARC AND TPR DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35205:SF1">
        <property type="entry name" value="ZU5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24809">
        <property type="entry name" value="DUF7708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7708" evidence="1">
        <location>
            <begin position="90"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="971" mass="109715" checksum="42F59726385A0F40" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIP7</accession>
    <name>A0A8H6FIP7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156907.1">
        <property type="nucleotide sequence ID" value="XM_037298272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIP7"/>
    <dbReference type="GeneID" id="59335780"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="413" mass="46805" checksum="A9F4AFBE09661B2A" modified="2022-01-19" version="1">MAFASVFAKTSGLISSSPQRSTSSYSSTTHTELEQQSESGSMSPTKRRTEKWLQEHTPKKHGSSHDLRKVKDGRIVRENGRDGRKKRASFWNLALWFSGYGKSQSNDREEDDLEGDTMIDDDASTAAPGYDNDPTLVVDDDNTGIKGDETAHALQKYNDRYLDYDDPRIQDWTEEERWLFAKLTNRGYEPLLHDTWILDYPTFPDPLFTNDENQVYINNIHSSIGRACRSLTDLILAGSRVRDQILSQAATEKPLFHAINKYYKWSIADGKVQHKDHIPVIAIASAKGQETVESVVGRITDQLHSLGRQYRDKWRHPSSHESDEHQEFTHELPTLTGFVIKYSIVAIVTCDIRVAGNPIRTLGTFDFKVIGQDVWHALAISIAMIRGRNYLMQLDEEGELGPEVIDYGSDPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIX5</accession>
    <name>A0A8H6FIX5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156981.1">
        <property type="nucleotide sequence ID" value="XM_037298346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIX5"/>
    <dbReference type="GeneID" id="59335854"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039146">
        <property type="entry name" value="GPANK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20923">
        <property type="entry name" value="BAT4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20923:SF1">
        <property type="entry name" value="G PATCH DOMAIN AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="136"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="233" mass="25800" checksum="DFD5C1563D8BDBDB" modified="2022-01-19" version="1">MSDKEDCSPWEQQRTFGAGVKRRRIDFVAAAAAPTQAIQLARRTTPSISPGDRYLNIVFKNGASTEDHDSTSTEVFKPGRSATQLQPDLKGPFCEICNLPIDAIGDPTSTVSTNHESTIAHMVCLTHSHPPSHLDRSRQGLKYLSSYGWDPDSRQGLGATGKGVRAPIKAKVKNDTVGLGAKTKGNKKPQGAKVERMDAKQVRKMDMEDRRKRERLREMFYRNDDVEKYLGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ18</accession>
    <name>A0A8H6FJ18_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157072.1">
        <property type="nucleotide sequence ID" value="XM_037298437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ18"/>
    <dbReference type="GeneID" id="59335945"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="331" mass="31729" checksum="1065EEDDD715DD6D" modified="2022-01-19" version="1">MADKEQEGASSAGGYLASAKSYIPGMGQVSDAASGGAGKAQEGLGAAGSATKDVTGYVPVVGSVVEGVGNTPVAQKIGGKEEEKGYLGSAYDYAGSALRTVYDAAGNVIGTVEEKAGTYLHGSENAAKAEGEEGDPTAKGEARQKDQEVIAKSKAEGATQETADKASETADGTAATAKETAGSLGQTASDTTDKVKEGHRGVEDNVESGAKDAVGQVRNATGTTDATEDANKGLEDTVGATKGTARGVEDAASETGGKVVGGVGKANTEGATDAAGGVKDGVTGTVGSVGEGAKGVASGGTDTVKGGVGALGSGAKSAGGALGGMTGLGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKI3</accession>
    <name>A0A8H6FKI3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157470.1">
        <property type="nucleotide sequence ID" value="XM_037295469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKI3"/>
    <dbReference type="GeneID" id="59332960"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="368" mass="39275" checksum="DC8AE5A7F9692F01" modified="2022-01-19" version="1">MLQSQENTDYNTLHSTEPGKEAIVARLQKHDSTVQTPKSGTDYENNSQKQKQAEKTGASERHQLMSSARKYRQPSAATIGVESSRAYWPSSPLQHGMELIDCDAFSAESNGEGHRLLISASDAFWGLEDDIASHLAWFTLRLKCQPGDIISVLSELLRSTQKGLQSGLTPSTSGTLHPIIPTASGKVVSPGKGQILSGGSKKLIGSNRSLSIQSIRALGHRRGFSFFPGDDSTNPILFNTVGGQYTDCETISIRPLDWQDTKQGEHNGSGASAKGGLSGGMAVGSRSQQPLAKSIVSLTAGSDVSRSPQPHGSGKSDLTTIIDGSSRSSSLSQRESLNSNVENDTVRKCFIRRGNSHLAVAAARAART</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKL7</accession>
    <name>A0A8H6FKL7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPI anchored protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157528.1">
        <property type="nucleotide sequence ID" value="XM_037295527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKL7"/>
    <dbReference type="GeneID" id="59333018"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034554316" description="GPI anchored protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="34091" checksum="593FB172E1A95C4B" modified="2022-01-19" version="1">MHSIRDILSMSLLLTLFSSFVHAHRLHQSPQNQEEESGFAGLQKLLDQVDAPSLHAALHDLSPKKFKHGMFQDDRTAVEAIHKDEPSLATSVVNIAKRNVMEDIKKDLAKRAQEISNGTTTTTTPAASVSESSHTGLATPVPQGGGLSSSTTSSPSTDTSTTIAGAGPSMPSTATSTSTSSNGAIVAGSDSGSTTSASTSAGESPSTTATSSASLTHGEVVTSTNSVGLTIVSTVGGGVHTISPSPGSSTPSSSMTQSSITSTVVHTSTLPNGSQSVVTAVTIVGAGAAAAGTPTGTAGVATAGPSSGSPRLQTGEAPMMRGWCKEMLLVVGGAVGVAMMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKX3</accession>
    <name>A0A8H6FKX3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157682.1">
        <property type="nucleotide sequence ID" value="XM_037295681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKX3"/>
    <dbReference type="GeneID" id="59333173"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034954595" evidence="1">
        <location>
            <begin position="20"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="184" mass="19988" checksum="A0CCCE307D8C8968" modified="2022-01-19" version="1">MRTAYCLMRLLQSLGASLAATVPATSLLTLYNGANINLTLPPNSALLSNPSLPSELVVPAPAAPYYIEEGRAFLKFYDYGAALPPAPAQELLRTALDAITHGHGVDSYRKVGREVIYSQTLDAGAGVLWLSVEPEANMTWGEFETAVMGAGRFLRRWDNVEFAVDVEMRMEAGGKVGTVYLSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL30</accession>
    <name>A0A8H6FL30_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004815</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157729.1">
        <property type="nucleotide sequence ID" value="XM_037295728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL30"/>
    <dbReference type="GeneID" id="59333221"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000024">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF20">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="35"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="424" mass="47524" checksum="683FDD2A72E86B2C" modified="2022-01-19" version="1">MVRSDPFQPAKRVASQKQDVWTIVNEAATASPVQPIINMGQGFFGYNPPQFVTDAAKEALDRVECNQYSPTKGRPRLKKAIADAYSPFFGKKINPDTEVTITTGANEGILSAFMGFVEPGDEVIIFEPFFDQYIPNIEMAGGKVVYASLQPPKEGATKTTSASEWAIDFSELEKSINDKTRMIVLNSPHNPIGKVFSKKELEHIGTLCVRHNIIILSDEVYDRLYYVPFTRVATLSPEIAKLTLTVGSAGKNFYATGWRVGWLIGPEHLIKYVSAAHTRICYSSVSPLQEAAAVGFERADELGFWEQSKREMKSKISRFCEVFDELGLPYSEPEGGYFVLANLSKVKLPEGYDFPPHVAERPRDFKLSWFCIMELGVAAIPPTEFYTDENAHLVEDWLRFAVCKDDEVLDGAKERLRGLKKYMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL51</accession>
    <name>A0A8H6FL51_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Delta(24)-sterol reductase</fullName>
            <ecNumber evidence="2">1.3.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004872</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.72" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157785.1">
        <property type="nucleotide sequence ID" value="XM_037295784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL51"/>
    <dbReference type="GeneID" id="59333278"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000246">
        <property type="term" value="F:Delta24(24-1) sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050614">
        <property type="term" value="F:Delta24-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000031">
        <property type="entry name" value="FAD binding domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040165">
        <property type="entry name" value="Diminuto-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801">
        <property type="entry name" value="24-DEHYDROCHOLESTEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801:SF10">
        <property type="entry name" value="FAD BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G14300)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="41"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="555" mass="63022" checksum="DBC7845FA93E0699" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYY4</accession>
    <name>A0A8H6KYY4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157080.1">
        <property type="nucleotide sequence ID" value="XM_037298445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYY4"/>
    <dbReference type="GeneID" id="59335953"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034542191" evidence="2">
        <location>
            <begin position="23"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="669" mass="69564" checksum="064ECCECC8D41193" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZH2</accession>
    <name>A0A8H6KZH2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_004271</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157191.1">
        <property type="nucleotide sequence ID" value="XM_037295190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZH2"/>
    <dbReference type="GeneID" id="59332680"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000007">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000037">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029184">
        <property type="entry name" value="Sas4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF4">
        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15460">
        <property type="entry name" value="SAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Something about silencing protein 4" evidence="13">
        <location>
            <begin position="252"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="11">
        <location>
            <begin position="659"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="12">
        <location>
            <begin position="1008"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="14">
        <location>
            <begin position="1122"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="342"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="577"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="831"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1208"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="259"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="609"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="834"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF15460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6229934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1273" mass="143085" checksum="E2108B00FC5C58AA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L0C0</accession>
    <name>A0A8H6L0C0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157820.1">
        <property type="nucleotide sequence ID" value="XM_037290946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L0C0"/>
    <dbReference type="GeneID" id="59328425"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="388" mass="44082" checksum="FF06DD196BDA4C48" modified="2022-01-19" version="1">MLKLRESDSSIVPDEALQDLERLIPLVSDLHTQLDNFSSTFCMERDIMSHAFRGWVDTYSKIVADSDFVEFSLVSLFLFPQNHGLRFFNHFFLVPFLPKPRIYNVQVKAEIQFQRERFLADTKEALGNIDGSLLSIQSSGNSSNLTELLGAATRGMMAYQQNLDQNMNALKDKVLFGGLIRTIFGESQEIKKLKQYHALVSHILVRLGSINATVARCEVDSRSLKVGLSQLRRSFEYSPNFEFSPAGLISIPPSLSAQLKSPPFTLLKQSSGFDNENGSKETKPFPSYDKWEYDLDKVISTNVLTRRTAIADWCQDGGNYVMRSGFLYTVCNIWSQAKLMSTLDDDLLSGVQRSWIMEREKAIHRYEKTIGLDFANIIHDVLHVYDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5S6</accession>
    <name>A0A8H6C5S6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006679</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147012.1">
        <property type="nucleotide sequence ID" value="XM_037297576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5S6"/>
    <dbReference type="GeneID" id="59335080"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF15">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6217577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="636" mass="70887" checksum="F3AFC9103589914F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5X2</accession>
    <name>A0A8H6C5X2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Osmotin, thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147052.1">
        <property type="nucleotide sequence ID" value="XM_037297616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5X2"/>
    <dbReference type="GeneID" id="59335120"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="24"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="98"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="207"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="35462" checksum="CB224E4EF75C1954" modified="2022-01-19" version="1">MKRPPLAYVSRRAETVPLVVRSLCSETIYPGIVTQAGTPPSSGGFELQTGQQMNLTVGADWQGRVWGRTNCSFNSQGTGPANAGGNNGGGSACGTGDCGGIVGCKATGETPVTLAEFTLATSSGQTFYDISLVDGYNLPLAIVSLYPESDNSSLTKIPPNLTNPICIGTAALLTAQGDTSDSNSGTNSSFPIPLDESVSVSDVASWCPWDLQVNIPTVPFDGVYSYPDTNIQRPGFDPCFSACAKYSQAADCCTGSYDSPNVCKPSSYSTAAKSVCPDAYSYAFDDQTSTFIIPSGGGFEVTFCPTGRSSNILKVYKQQLAELSETGSVSESSLEAMQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6E7</accession>
    <name>A0A8H6C6E7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147263.1">
        <property type="nucleotide sequence ID" value="XM_037297138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6E7"/>
    <dbReference type="GeneID" id="59334640"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.30">
        <property type="entry name" value="Microbial ribonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016191">
        <property type="entry name" value="Ribonuclease/ribotoxin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53933">
        <property type="entry name" value="Microbial ribonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034278494" evidence="3">
        <location>
            <begin position="22"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="177" mass="19283" checksum="8C69B27FCAF8C53A" modified="2022-01-19" version="1">MFYASVICISWAFASLPLTYCIPVGGAEPLQVVDVVGAPTDDVSCPVHTNQYFTAGYVTGNIADIVRNGKKGSYPKPFNPDSGKADQFNWDLNDCGTRQTNGETILELPMFIDSTNNDEETYWSQNPASNNNKPGPYREYYIVKSNGASSQTENTATYCGAYAHKDLSSNGDFVACT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6W3</accession>
    <name>A0A8H6C6W3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147206.1">
        <property type="nucleotide sequence ID" value="XM_037297496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6W3"/>
    <dbReference type="GeneID" id="59334999"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046536">
        <property type="entry name" value="DUF6601"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34414">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34414:SF1">
        <property type="entry name" value="SUBTILISIN-LIKE SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20246">
        <property type="entry name" value="DUF6601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="132" mass="15133" checksum="3956A726ABFE3BA7" modified="2022-01-19" version="1">MSPPFAEAHIRLTRYTHGISTMLFDFLNNDILTLDLDRMAPNLWLMDTPSGSHISPLHNQKIKSRKIVITEDPGLHLVSSDNRVFIKPFPPYLQFLYLLGKILDRAEFNILPVHIPTSQDIRIGSITTLELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C766</accession>
    <name>A0A8H6C766_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147670.1">
        <property type="nucleotide sequence ID" value="XM_037297096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C766"/>
    <dbReference type="GeneID" id="59334598"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="685" mass="75533" checksum="86CAAA0AC1F080F3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C791</accession>
    <name>A0A8H6C791_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006341</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147364.1">
        <property type="nucleotide sequence ID" value="XM_037297239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C791"/>
    <dbReference type="GeneID" id="59334742"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="344"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="319"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="585" mass="65384" checksum="44F16F2483F07AD3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C816</accession>
    <name>A0A8H6C816_9LECA</name>
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        <recommendedName>
            <fullName evidence="2">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">HO133_005748</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147836.1">
        <property type="nucleotide sequence ID" value="XM_037296655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C816"/>
    <dbReference type="GeneID" id="59334153"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010605">
        <property type="term" value="P:negative regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="203"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="676"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="767" mass="86210" checksum="9681B751AB6F1B12" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C821</accession>
    <name>A0A8H6C821_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005914</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147999.1">
        <property type="nucleotide sequence ID" value="XM_037296818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C821"/>
    <dbReference type="GeneID" id="59334319"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051701">
        <property type="term" value="P:biological process involved in interaction with host"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000606">
        <property type="entry name" value="C2H2 transcription factor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000758">
        <property type="entry name" value="C2H2 transcription factor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="141"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="204"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="477"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="532"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="644"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="307"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="537"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="604"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="645"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6218564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="709" mass="75987" checksum="8D76BABA7921FEF2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C851</accession>
    <name>A0A8H6C851_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005986</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148070.1">
        <property type="nucleotide sequence ID" value="XM_037296889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C851"/>
    <dbReference type="GeneID" id="59334391"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="5"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="306" mass="34523" checksum="93253E53A1581C16" modified="2022-01-19" version="1">MPRNISNIFKHVWLCMVFVGPTFTFIKLSLLFFYRRLFLVNQKWLRVAWWANMVYVILWFFGATGFYIFQCWPVQWYWTRYYQRYHVGPPIAMKGQCKATTVAHVAMPLIFSLISDVALLLLPLTAISKLQISLKKKLGLASIFSVGALACLLELARIVELEVDTDDQSDPSYGVVVFLILSVAEEVCAIICGSLPVVIPQLFREYKSSRERSSQKKDRSYSMDLGAITQSRSMVRGFEKLGEGSGDRAYESRNLAGERIDWACGSIPANTVVAETLPHTEDPGDAQIVVQRNYEVTVGGSVPQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C855</accession>
    <name>A0A8H6C855_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005728</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147816.1">
        <property type="nucleotide sequence ID" value="XM_037296635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C855"/>
    <dbReference type="GeneID" id="59334133"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="271" mass="31953" checksum="F459369CF106C9A4" modified="2022-01-19" version="1">MPPPKQLSYQVQYWKKPMGINDHHNFPISDTAAAEMRQNATQNLSRLPSQLHQLAVGSTWERSADQSMREYQLLDYAAQNIDILKAMNIRDRDRDQEEASRKKKEEAPGVLNAIKYWVCPQEMKKTRNDMRELRERGRYARCVKDCMPEILDGTDMRWSGTPDFVYVMKNVEWIMEKARATATKVQTDNPDGHWAIELLNFYSDSWIVWLDDFNENFQTNWYDLDKKIEGAKQRAEVAEEIVEEIEKERRMRMPVGLAWGLASENFRERDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8I5</accession>
    <name>A0A8H6C8I5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005362</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148254.1">
        <property type="nucleotide sequence ID" value="XM_037296272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8I5"/>
    <dbReference type="GeneID" id="59333768"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03192">
        <property type="entry name" value="GST_C_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000051">
        <property type="entry name" value="Glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF263">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="115"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="29729" checksum="67B947EA293860BE" modified="2022-01-19" version="1">MTSSPASKRQKHSSASPSPPYELLYWPTIPGRGEHIRLCFEETQTPYTDTCNADPQTGPQTLRSLISGANDGDATNPPPLAPPALRHGDLLLSQTPNILLYLGPKLGLAGPGGGDGDAVYHVNQLALTALDGLSDEAHDTHHPVATGAYYEDQKEEAKRKAADYISNRLPKFLGYFERVLRGEASGGGEWLYGGQLTYADLVLWQCVDGVSFAFPVAVKQMREGGKYGRVFGLYERVKGRENIAAYLKSGRRQKYSMGIYRHYPELDAEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8J8</accession>
    <name>A0A8H6C8J8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005388</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148280.1">
        <property type="nucleotide sequence ID" value="XM_037296298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8J8"/>
    <dbReference type="GeneID" id="59333794"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031468">
        <property type="term" value="P:nuclear membrane reassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01770">
        <property type="entry name" value="UBX_UBXN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000179">
        <property type="entry name" value="Plant UBX domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.210:FF:000002">
        <property type="entry name" value="UBX domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.210">
        <property type="entry name" value="SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036241">
        <property type="entry name" value="NSFL1C_SEP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012989">
        <property type="entry name" value="SEP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333:SF20">
        <property type="entry name" value="NSFL1 COFACTOR P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333">
        <property type="entry name" value="UBX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08059">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00553">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102848">
        <property type="entry name" value="NSFL1 (p97 ATPase) cofactor p47, SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51399">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SEP" evidence="4">
        <location>
            <begin position="208"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="3">
        <location>
            <begin position="327"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="276"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="408" mass="43218" checksum="E3E14B9582EA485E" modified="2022-01-19" version="1">MAEGQPDHDALISEFVGLAGVTPQVAQEYLANSSWELGSAVTEYFNRSEGTEEDDQAPEAPAVTSSAPQPIPASSSMPPPPSATQPPKKKFATLGDLSGGGGPSHAGHGHGHDDEDDDDDFEPSDDDNPDLYAGGEKSGLAVENPGTAEKLRKKIIKKAQKNTPRPGGDDPRGPSSRFTGTAHTLGGDDAPSQTIEDPNASRPQHAPPVERHLHFWADGFSVDDGPLYRSDDPANAAILAQIKTGRAPLAILNVEMAQEVDVKLEQHSEKYVQPKKKYVPFGGGGQRLGSPTPGISNRTAEPAPAPAPAAAPASSATTQAVVVDVNESQPTLSLQIRLGDGTRLPARFNTTHTIGDVYSFITASNPQSRTRSWVLMTTFPSKELSDKSQVLGDLSEFKRGGVMVQKWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C972</accession>
    <name>A0A8H6C972_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Altered inheritance of mitochondria protein 24, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005108</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AIM24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148718.1">
        <property type="nucleotide sequence ID" value="XM_037296020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C972"/>
    <dbReference type="GeneID" id="59333514"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959:SF2">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="TRAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="378" mass="40935" checksum="EEF96FF1DF880C47" modified="2022-01-19" version="1">MRASLRGSEIIKSRCSRAPSVWHSSPAIWRRDLRISTPSADALDIVPSEPVPSTSGATSDAKFEVIGSPFSLLSVSLSASQNLYTRKGSLVGVGGKAENAVSTLSVLAPFRRAFLQIPFLYQRISSTTPITALISTKASRTSMTTLNLDGTLDWMVTGKALLAWTGQTLSITPSVNRNMSFAHWGNSQVTGRGLLALAGQGSISQLVLQAGESYVVHPSNVVAYSMNANPPLPHRLRSSSLRFQIPEIGVSNLLPDTRFIRAMRDSRTWRTFTTILFNVRTWARATIWGDRLFLQFHGPTTILLQTRAARILDVLTSENVNEIADTQPGVVQPVFTLPTERSTTDSAKKSKAGSAVTMKAPRMSTASIGTDGKVTFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9J9</accession>
    <name>A0A8H6C9J9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mediator of RNA polymerase II transcription subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mediator complex subunit 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED9</name>
        <name evidence="12" type="ORF">HO133_005058</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9J9"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.10">
        <property type="entry name" value="Mediator complex, subunit Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011425">
        <property type="entry name" value="Med9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07544">
        <property type="entry name" value="Med9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="135" mass="14266" checksum="B5C76D6B43EBB910" modified="2022-01-19" version="1">MPTATTPPPSNTLPPPQLFDILPPLHSLLSRLLLPPSADGLTPSPDTITPVSPKDLATAASSITNKIQKARVAVRELPGVGMGIEEQEDVIAELEEESRRLRGVEEGIRKAARNRLEGAASEVEAGIADDGAMET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA66</accession>
    <name>A0A8H6CA66_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149124.1">
        <property type="nucleotide sequence ID" value="XM_037295077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA66"/>
    <dbReference type="GeneID" id="59332567"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="466" mass="52605" checksum="104A64AA010F76F3" modified="2022-01-19" version="1">MSSTSQTPSNCPFLLLPLELRDRIYTQLLSIKHTRTPKDDESCLRSTTFYNWNLHPTIFRVNRQISQEAMAVMARENEFVVIERAKELEQGPEDVNKEDTTIMVYSVALWPGKATKKPVVPGERMRMKLERAEDSEMDMDVHVILAEELHDVCVGLSTFQDPKGKYRTSGLSCSIQVHPPPYAETPTAIDIRERALVEALTKFRHFKDVSIQGTRPAIDHQISRQLTRHSFDRGLVLVTIDELITSGDQASSNGDHGIATARYQRAHEYFHHSVSHERHVFTNPADVSAFEFKIMQHRALNWIEAGNFSDALEAAQIALYVANQLFRLNAPMSTGPPTRSGSRISAGAARKQTCDCIKEGAARYRQRIKAEDIGRCYFYKSISEHVVHGDGATQQADDDKFTAIGCCVVSETMTENVPTELLQLEIRTMEKLLGGGGEEVEEEEGDEEGWVDEEWEDDEGENDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA74</accession>
    <name>A0A8H6CA74_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Heme haloperoxidase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003529</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the chloroperoxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149139.1">
        <property type="nucleotide sequence ID" value="XM_037294451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA74"/>
    <dbReference type="GeneID" id="59331940"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.489.10">
        <property type="entry name" value="Chloroperoxidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000028">
        <property type="entry name" value="Chloroperoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036851">
        <property type="entry name" value="Chloroperoxidase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33577:SF15">
        <property type="entry name" value="HEME HALOPEROXIDASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33577">
        <property type="entry name" value="STERIGMATOCYSTIN BIOSYNTHESIS PEROXIDASE STCC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01328">
        <property type="entry name" value="Peroxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47571">
        <property type="entry name" value="Cloroperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51405">
        <property type="entry name" value="HEME_HALOPEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034303301" description="Heme haloperoxidase family profile domain-containing protein" evidence="8">
        <location>
            <begin position="19"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Heme haloperoxidase family profile" evidence="9">
        <location>
            <begin position="119"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="488" mass="52409" checksum="C22F3A98EFC545A3" modified="2022-01-19" version="1">MKTAYFALGAGLVARVFAACPFEMLKRSGVLTEADLAKFEAVKRDPKAAEVLFQAHKREAAPDPSPAGIIGPIVNGVLDLPYGGGLLNGALQELTGELQAVDVPTPQIITLEQIPGNDPAHQFQAPGPTDVRGICPTLNALANHGYISRDGITSFAEAANAVQIGYGFDYSLSVFLSALGLMAGGDLVSGKYTIGGADARVPNTIGPAGGLDKHGVFEIDGSITRQDVYFGNNANFLLQRWQEYVNETSQTGGLFNQALNAIDNGYRYDISRETNPDFLAGVIWFLVSHAERVFVYEGLANGTTGIPDYNSVAPFFLNETFPENWYRRGVPFTLVGAMTQAIEMFLMNPREIGGNEGLGNFVPLGFFNVTNQTPQELGCFILENLLDVAPDQLAPELYDNFELATGFIKGTNRPLSFALPPRRNLTPECLSGTIAPFFINDGYFNCNAALNFVKPGQSAGNSDGSVSSSGSPVRFSAFPWYCLPAADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAF0</accession>
    <name>A0A8H6CAF0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148986.1">
        <property type="nucleotide sequence ID" value="XM_037294939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAF0"/>
    <dbReference type="GeneID" id="59332429"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="63"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="248" mass="27424" checksum="A33810F4973E1A16" modified="2022-01-19" version="1">MCDGINDDVTLLFYENVDVVDYEETRKLFMAWTGCRDKRGLTICLFDMKYLTSNLGSFKKSCDFSSIAQPTPTGLPSAVTLRAVAVFEGMVRFVWPLCSRIRKRPNRVIVTGAPSYLPRLWIWAKDWIDPLTASKLMMPTEDEVLPILATYIDAANIPKKYEGEYGFEHGRQPDLDPAIDEIIDWLAPCSSSFPAGSMKLVFSSIGMRLAAAAGSIDGTQRTLLAGIVHPDRLVTTQSDDAAGGANKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAF6</accession>
    <name>A0A8H6CAF6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149425.1">
        <property type="nucleotide sequence ID" value="XM_037294737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAF6"/>
    <dbReference type="GeneID" id="59332226"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="114" mass="12595" checksum="6078901043F1FE60" modified="2022-01-19" version="1">MARAWKTQGKSTVKLPDKKKNSKNLAGTQARYNYISGISEGQYRYSTVKTSIWPGNTSDMVFLNELKPSDIRLLPLHLGKSSPIKACKDYECLETQGKQWACISGLPAIGAMAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAT0</accession>
    <name>A0A8H6CAT0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003733</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149343.1">
        <property type="nucleotide sequence ID" value="XM_037294655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAT0"/>
    <dbReference type="GeneID" id="59332144"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000138">
        <property type="entry name" value="ATP-dependent Clp protease ATP-binding subunit ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050052">
        <property type="entry name" value="ATP-dep_Clp_protease_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102:SF7">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="172"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="6">
        <location>
            <begin position="453"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="31"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="284"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="565"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="567"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="602" mass="64563" checksum="9337D76F94F2A842" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAT8</accession>
    <name>A0A8H6CAT8_9LECA</name>
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            <fullName evidence="3">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
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        <name evidence="10" type="ORF">HO133_003651</name>
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    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037294573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAT8"/>
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    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0436">Ligase</keyword>
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    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="112"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="168" mass="18602" checksum="33EF4D2C847EC622" modified="2022-01-19" version="1">MISQLQRRSMVVKRLKVFQIESIVRGYITGSAWSSYQENGTVCGRGLPPGLQESEKLQQPLWTPCTKAEVGGKDENISPAEAARIVGQAYADQIEQLSLELYKEANTYAAERAAAFGVLKDWLVKNGMKGKEMVEMPDDVALKSIDRYKRAYRSIVGKGWDAAEEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAU8</accession>
    <name>A0A8H6CAU8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003269</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149573.1">
        <property type="nucleotide sequence ID" value="XM_037294192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAU8"/>
    <dbReference type="GeneID" id="59331680"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="531"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="708"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="585"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="719"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="813" mass="90139" checksum="74584CA95B929E78" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-06-18" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAV0</accession>
    <name>A0A8H6CAV0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7892 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003301</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149605.1">
        <property type="nucleotide sequence ID" value="XM_037294224.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331712"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="DUF7892"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="880"/>
            <end position="1031"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
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        <location>
            <begin position="363"/>
            <end position="403"/>
        </location>
    </feature>
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        <location>
            <begin position="729"/>
            <end position="779"/>
        </location>
    </feature>
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        <location>
            <begin position="1044"/>
            <end position="1122"/>
        </location>
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        <location>
            <begin position="1198"/>
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        <location>
            <begin position="1292"/>
            <end position="1323"/>
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            <begin position="1348"/>
            <end position="1412"/>
        </location>
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            <begin position="1426"/>
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        <location>
            <begin position="1574"/>
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        </location>
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        <location>
            <begin position="499"/>
            <end position="532"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="20"/>
        </location>
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        <location>
            <begin position="372"/>
            <end position="384"/>
        </location>
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            <begin position="390"/>
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        <location>
            <begin position="732"/>
            <end position="757"/>
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            <begin position="1294"/>
            <end position="1309"/>
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            <begin position="1368"/>
            <end position="1380"/>
        </location>
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            <begin position="1436"/>
            <end position="1455"/>
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        <location>
            <begin position="1469"/>
            <end position="1478"/>
        </location>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF25422"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6220170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1598" mass="178589" checksum="492161382FCECE11" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB13</accession>
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        <recommendedName>
            <fullName evidence="5">Striatin N-terminal domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="6" type="ORF">HO133_003868</name>
    </gene>
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        <name type="scientific">Letharia lupina</name>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6220043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="7"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="1282"/>
            <end position="1308"/>
        </location>
    </feature>
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        <location>
            <begin position="35"/>
            <end position="175"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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        </location>
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        </location>
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        <location>
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        <location>
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        <location>
            <begin position="1252"/>
            <end position="1262"/>
        </location>
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        <location>
            <begin position="1263"/>
            <end position="1273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08232"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6220043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1326" mass="141424" checksum="03D7C73FD029CF2B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB51</accession>
    <name>A0A8H6CB51_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037294357.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59331846"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="371" mass="42372" checksum="44BB2510897A6AE9" modified="2022-01-19" version="1">MPVRFYSARDQSQQNQHILTEFAARFSKAIRKAEFHTIGVGWDDDSPNQIHPLLGLLDFLEDQKSKLPPRIMDINPRSYHLEDDKSYLSEQQEEAEIERIIERHGDRITAMVSETHLKILSNASTTEMTAWNDGIREGEPAATVILLLALYPHFESLRFYSLEYWREINFETIITSLTTAATDSRTNKLGVISELSEFTLNHWTETSGVKSKVELSGPSMALPKMRSIEAYNVHRRNNQWSWGTGFSHVTDVKLEVCDIDTTTLTNYIGAAKALETFTINTEIPHVERGSEEEPVEKICGAMFKDLPKLRKACLPNLEEICFEYVEDDDLVEEEGHRELRSRCKEAGVKVKSIGWVKGREYTAGRFNIVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBD7</accession>
    <name>A0A8H6CBD7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_003295</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149599.1">
        <property type="nucleotide sequence ID" value="XM_037294218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBD7"/>
    <dbReference type="GeneID" id="59331706"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009582">
        <property type="entry name" value="Spc2/SPCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 25 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06703">
        <property type="entry name" value="SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="180"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6220164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="231" mass="25608" checksum="6E11765600F28F66" modified="2022-01-19" version="1">MAATKIAVYSISDLKNTTDDALPNYLTSLKFKQSHFLTDVRLALGYSAVVIAAITFYADYKLGWDKTKYWTFWAVLVYFTLNGALTFWIWGVEKGKVFAGQIDHPKVGRKSPLSIASSVAKHTPIYNITVEYSNSKGETKTLKLSSPFTRWFDVNGFFVAKPFQQWLALEIPLIGQVDPKNKDDGAKSAASANGESEVDGPVEDEKYDTPKKSTSSDAAPTSTRSRRGKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBP5</accession>
    <name>A0A8H6CBP5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002932</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149934.1">
        <property type="nucleotide sequence ID" value="XM_037293856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBP5"/>
    <dbReference type="GeneID" id="59331344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056599">
        <property type="entry name" value="AAA_lid_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054289">
        <property type="entry name" value="DUF7025"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411:SF1">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05752)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23232">
        <property type="entry name" value="AAA_lid_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22942">
        <property type="entry name" value="DUF7025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="787"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1010" mass="113557" checksum="07E2912F1F1EA1A2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBV3</accession>
    <name>A0A8H6CBV3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150128.1">
        <property type="nucleotide sequence ID" value="XM_037294050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBV3"/>
    <dbReference type="GeneID" id="59331538"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="159" mass="17805" checksum="C818E6127021E0CD" modified="2022-01-19" version="1">MTLDVMNRAVTDSCALNFQTSDASPTKAIAVGPSWPGLDYSAQIYQDNWGNFVSLWANFRVDRASNREPYLLYYQPCISLLERAEIGNGTESHGGMKDCTNTTGNGLLRMEDGELRILELSLRSLSVHLAFIVYRTGRDLEVADFSHWKEKGGKEHGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBV8</accession>
    <name>A0A8H6CBV8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Palmitoyl-protein thioesterase 1</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyl-protein hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003055</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the palmitoyl-protein thioesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150057.1">
        <property type="nucleotide sequence ID" value="XM_037293979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBV8"/>
    <dbReference type="GeneID" id="59331467"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000107">
        <property type="entry name" value="Palmitoyl-protein thioesterase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002472">
        <property type="entry name" value="Palm_thioest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF8">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02089">
        <property type="entry name" value="Palm_thioest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00414">
        <property type="entry name" value="PPTHIESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034686973" description="Palmitoyl-protein thioesterase 1" evidence="9">
        <location>
            <begin position="28"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="333" mass="37655" checksum="66EE90613394719A" modified="2022-01-19" version="1">MGMGIDMGMTFLYLWALLPIAWRFVTALPPPQQPILPTESTPIASSAESANLPLVVWHGLGDNYKAEGLQSIGELLNDTIGNTTTYYIRLDGDPSADRTATFLGNVTLQVQKVCDDLASHPVLSKAPAINALGFSQGGQFLRAYVERCNSPRVENLVTFGSQHNGISEFQECATNDWLCQTWRGVLKGNAWGSFSQSRLVPAQYYRDPEDLDNYFEYSNFLADINNERKVKNDTYKENMKKLGRFAMFMFANDTTVVPKESAFFSEVNTTSEEVTKLQERDIYQEDWIGLRFLDEKRRLDFRIAEGGHMQLSDNILTDAFNRYFKSRNKGGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC53</accession>
    <name>A0A8H6CC53_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003157</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150159.1">
        <property type="nucleotide sequence ID" value="XM_037294081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC53"/>
    <dbReference type="GeneID" id="59331569"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF184">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SYNOVIOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="28"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="244" mass="27028" checksum="C0C68F1C793512FB" modified="2022-01-19" version="1">MPPSCIVENFLSSLPVVNLEDLWAHRQCDICQESFFKWAEKPLKLPCGHIFGSKCIAEWLSPTAVDLKPKNTCPICREVLFSQSGRRFWDSTSLVPGTRGVRLPVPLLTSAARGGRQDAIIHPGLLARLAKAQNRGSAGGRNALGPAAEALALLRVIIRELSGNKAGRETAESIPVSVIDDAVHSLAAQMGRLYEHHEPRIDEETTSISWDSEGPEVSLLRDPVWARWCEWQLQELVYMELNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC67</accession>
    <name>A0A8H6CC67_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150155.1">
        <property type="nucleotide sequence ID" value="XM_037294077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC67"/>
    <dbReference type="GeneID" id="59331565"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="516"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="704" mass="77478" checksum="B80FC04495052D4C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCD1</accession>
    <name>A0A8H6CCD1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150116.1">
        <property type="nucleotide sequence ID" value="XM_037294038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCD1"/>
    <dbReference type="GeneID" id="59331526"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="412" mass="46223" checksum="66C83F4D06F1533A" modified="2022-01-19" version="1">MTDLAELAMEGVPLVTEHYDKVYDPLKDKTKQGIQKVKKMRNKSNTQGGGYESETDEEIEYDAPPRRNYTEPTRRRSPRGDDRRRSRRDYDDVVEERYVYKGPNKDRAKSMGRDGWTGGRGDGRRGGRRDYSDSESSVSPRPRERRKSLGEKALMALGLGGAAGAAAGSNRDKRRSRSRRRRSPSTSSSSSDDGYYRRRVNRNRGPSGDYYDDRTPARYKPAGYLQNGERDGGSERGGGQQVARRDNRSEVGSRKGGEGKKDSSSSESSDVCSSSEDERRTKKMKGKEYLTAGLAAVATIHAAHSVYASMEARDKRHVEVAKGELSPEEARKKKNKARIQDAAAIGIAALGIKGAYSEWQEVQENRHELAEELEERKKRHEKRVKRQEKQAKGGSGGGRSRSEAPNRRRDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCN0</accession>
    <name>A0A8H6CCN0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDP-mannose transporter</fullName>
            <shortName evidence="7">GMT</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_002753</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in the import of GDP-mannose from the cytoplasm into the Golgi lumen.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasmic vesicle membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TPT transporter family. SLC35D subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150507.1">
        <property type="nucleotide sequence ID" value="XM_037293678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCN0"/>
    <dbReference type="GeneID" id="59331165"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="389" mass="42766" checksum="61E025113225F9EE" modified="2022-01-19" version="1">MDALNDDNEKFRLVSSNTREEAQRPRSESALSDRTLLNMSDSETEEKRRSGDLEKEGLVEQLPKVVTKEHATKDPYARAKILMWMFVNTLATVTIVFCNKAIFSDKSFSQCQAAFASFHFFVTAATLYLLSRPAVGMFEPRKIPIMNMLPLAIAMAMNIVGMNVSLQVSTITFYQITRVLLTPIVAIINFFFYKKTIPRMAVYALIPMCFGVGLISYYDQKSAPVPGAPIKAVGALSVLLAFGSVTVSGIYTIWIGTYQRKYEVNGFQLLYNQAPLGGVLLLYVVPWSDKFPALDQTPAVMWGLILLSGFLAALINLSQFFIIGGSGPLESTVVGHLKTCLIVVIGAFSGGKVITDKAAFGILLALISIFSYSAIMMRHTNLASTVKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEG4</accession>
    <name>A0A8H6CEG4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Chromosome condensation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001176</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Fluoride channel required for the rapid expulsion of cytoplasmic fluoride.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:76160"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151525.1">
        <property type="nucleotide sequence ID" value="XM_037292106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEG4"/>
    <dbReference type="GeneID" id="59329592"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="FluC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259:SF1">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="317"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="490"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="57640" checksum="E5971AC07EEB09E6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEJ7</accession>
    <name>A0A8H6CEJ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit L</fullName>
            <shortName evidence="4">eIF3l</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001774</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151241.1">
        <property type="nucleotide sequence ID" value="XM_037292702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEJ7"/>
    <dbReference type="GeneID" id="59330188"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03011">
        <property type="entry name" value="eIF3l"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019382">
        <property type="entry name" value="eIF3l"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10255">
        <property type="entry name" value="Paf67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="483" mass="55734" checksum="1EC4CA0B45EA9A6C" modified="2022-01-19" version="1">MALYQRAPYETANRQDLVEDESDREEEALVEDYKEQVHFDDGMSELDRTASFGGAAAGGGPQDMQAQLAAAATPLEYGAALDTKFASYDNYCNLFHYILNSEGPVDLEVPTYYWAWDVIDEFIYQFESFCRYRNRIARTESTSEEAQLLRDNPNTWGCYSVLNVLYSLIQRSQINEQLDAMKRNEDPNQVAGEYGNRPLYRMLGYFSIIGLLRVHCLLGDFSLALKTLDDIELNKKAMFARVMAAHFTTYYYVGFSYMMMRRYADAIRMFSHILVYVSRTKNFQKNAQYDSVSKKNDQMYGLIAICVAFNPTKLDDTIHTALREKYGDQLVRLQRGGPEALPLFEDLFRSACPKFISPTPPDFDTPDLNVDPVDHHCKIFMEEVKNTMWSPTVKSYLKLYTTMDLKKLAGFLDVEPEKLRSWLLVNKQRSRQVRWSEGALLDGEVVNGSDLDYAMQDDLIHISEAKVGRRLVDWYLRNLARTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFM0</accession>
    <name>A0A8H6CFM0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000735</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152034.1">
        <property type="nucleotide sequence ID" value="XM_037291672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFM0"/>
    <dbReference type="GeneID" id="59329153"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141571">
        <property type="entry name" value="Pentapeptide repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="493" mass="54783" checksum="2248CB184E156C5E" modified="2022-01-19" version="1">MLWEKLLDGRLLDGRLLDKKLLDSTVFDGTVLDGTVLDGTVLDGTVLDGTVLDGTLFDGTLLDSTDGRLLDSTLLDSTLLDERLLDGRLLDGRLFDGRLFDRTLLDSTLLDSTLLDRTLLDERLLDGRLLDSTLLDSTLLDERLLNGRLLDSTLLDRTLLDERLLDGRLLDSTLLDSTLLDSTLLDRTLLDSTLLDSMLLDRTLLDERLLDEKLLDSMLFDGTLLDSTLLDSILLDRTLLDERLLDGKLLDSMLFDGTLLDRTLLDSTLLDSILLDRTLLDERLLDEKLLDSMLFDGTLLDERLLDGRLLDSTLLDDKEIVGDGVLELIEELIEELIAELIEELIEEIGDELGGLLEDVLEERIDAATEYVLITVLEDVVKEVLEEMTVNTLDETIDGDDDKLEEPDDRVDSDDELVITEEGIEDEGVAVVELDVPELDVPELAGVLELITVGPLGDSVDEGVTMVELEVPELEVPELEVPEFAGVLELTDVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFU9</accession>
    <name>A0A8H6CFU9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-threo-3-deoxy-hexylosonate aldolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000858</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152154.1">
        <property type="nucleotide sequence ID" value="XM_037291792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFU9"/>
    <dbReference type="GeneID" id="59329276"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00408">
        <property type="entry name" value="DHDPS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF24">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHETASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_3G11920)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="2">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="34053" checksum="DC7C81BC98FE37A0" modified="2022-01-19" version="1">MATKSRTPLRPGIYCPTLTFFDPTTEDLDLPTIAKHSVRLARAGLVGLVTLGSNGEAVHLSRSEKSAVTRTTRQALDEAGYRDVPVICGAAEQSVRGTLELCREGAEAGADYVLLVPPSYYRAAMNEGQLEEYYNAVADESPVPVLLYNYPGAVAGIDMDSDFIIRVAQHSNIVGTKFTCGNTGKLTRVASATDACTPKKKGSGFMAFGGMADFTVQTWVSGGSGIVSGAANVMPKIVVEAWNLWTEGKTEDAMKLQEVVSRGDWLLGKPGVPGTKAALQRFFGYGGYARKPLRRVGGAEVDSLAEKMKEVMDIENTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG49</accession>
    <name>A0A8H6CG49_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152183.1">
        <property type="nucleotide sequence ID" value="XM_037291821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG49"/>
    <dbReference type="GeneID" id="59329306"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="448" mass="49955" checksum="38EC4B5632806114" modified="2022-01-19" version="1">MAISRRFSGFLAISAATLFLVGFFLFGSNRNRQFSYKSLLFSETIVSDDTNSSDTSFATNDGGAVKSPLSDVSQKPLSIAIVETTGYHDEVVAAFVHSFGSQKRSNLSLFQQNQRYGIEKIMKDFKLPKPVPAAQPPDMLLADDATKLDILVLTTCEYDRIKIASQLEILLKEGKTQLLCVVHHADHWANEDHQNFMAPWIDKGLVDFITLSQHTATFLQETGMRTWKTKPPIRPFAPVFPVNLPKLPTAVDADKGELGFGLQGLYESTRRNYETIFAHLQKFIDSRSSERVGTMNSNVTLHLLGQGIHPEVPSGLISHVSFDESLDYDEYYSILSRTFALLPAFANDQYLDRKASSTVPAGLIGGTPLVATKEIVAAYSYLPEDAVYLQDKDETEFDVIGRVLKDAAKHRIKKMQMVRNKCAELVKGNSGLVEGWIKEILQKEHVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG55</accession>
    <name>A0A8H6CG55_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001009</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152304.1">
        <property type="nucleotide sequence ID" value="XM_037291942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG55"/>
    <dbReference type="GeneID" id="59329427"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22749">
        <property type="entry name" value="Otubain_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.60">
        <property type="entry name" value="Peptidase C65 Otubain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.20">
        <property type="entry name" value="Peptidase C65 Otubain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042468">
        <property type="entry name" value="Peptidase_C65_otubain_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042467">
        <property type="entry name" value="Peptidase_C65_otubain_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF15">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="8">
        <location>
            <begin position="49"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="350"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="351"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="425"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="457"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6222958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="472" mass="52311" checksum="41BECD2024B73051" modified="2022-01-19" version="1">MERLSNDWRPDAPGPLVGRQQSTSALIAEYAQADPVYVNKTKALPQIYSHYRTLKGDGNCGWRALAFGYFETLLRLGDASRMLEEAARFRAFEALMHAVGIPDLYEDFIEPTADLLEQQAATLPNHDGGAAVLAIFNEDAPSSQLTYSFKLITSAWMKSNAESYAGFLPDMDVHTYCARNIEPFSIELDHVGLQALATAIINEAGMAIEVLYLDRSAGEEVTPHRLPVLDADGNEKKGGPTIRLLYRPGHYDLLYKTEDVAEMSRHTLSHPEIRLVSAPQIAVSNHAQFSHIGDMDILADIPGFSSSMFQMPFTPTSTEPYQSKVETFSSAPCTMTPTPPLYPSYQSKAETFSSTPRTATPATPIYPSVDPAVKEEYSPKAATASPISPAPKELPIQADFKPRTKQQRKYDRRFKAQGEQIQSDVLKDCSPDRSKKEDPAHFTNQAFQPQIWQPGRVYEKAGEPEASRRASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGR3</accession>
    <name>A0A8H6CGR3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152302.1">
        <property type="nucleotide sequence ID" value="XM_037291940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGR3"/>
    <dbReference type="GeneID" id="59329425"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="345"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="499" mass="54225" checksum="9C3BD5642A50F06D" modified="2022-01-19" version="1">MSDPTTIPDNLHNPNDFTAANLATTFPTSPGRPTPNSAKKRRRMPDLSRPTATPSRGAQMSMIFRDAATSLQGSRSHTYPSWSNIKRSRLPLSQARSIKFGSTCQDDTIVSSGLGRSPKISQYLGESCEPGWASSEVSRHWASGIGPHSPTPTPFTKAGCATPETLTNQCLGEAIDVGGMCLDDALRVSSASLPSQLEEEGKEPISSGFATPIAPTPSFVENPEEVKYPILENWPSLRSSSDACSPGSDRDDLHSTHDVPFILPFPHSDAEETQRCHIDTWLNRIVEATKSGHSRSPKQCCSTEDLLMDDAPFSQSIAPNLSSPKRPRVSVSNLKQDLQSPLGASSNKENISPSKSSSSPTHPPAQHPQALTPSHSRQTHSQATLQHTKALHFAHPSTLQGHLSLPPKRKRARVDGIASSRAETEMSTARRDFTIHEDQLAGALAQLSPDVDRHRRGRGPKRERCISYWDEDILQPGSQCVPTDVNGETLEKGKQVLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH41</accession>
    <name>A0A8H6CH41_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000219</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152594.1">
        <property type="nucleotide sequence ID" value="XM_037291159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH41"/>
    <dbReference type="GeneID" id="59328638"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF9">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="11"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="474" mass="51692" checksum="20B71E92F8193730" modified="2022-01-19" version="1">MSKKLSVYSTQASHSGQMIQTDAANNFWEQIEYAYHQNISFLGTGGGHGYSATLDTVENGIEIDLGHFNTVSIDKDANTMTVGGSVHFANITGLLYDAGKEFRLFTEVGSCSCVGLVGASLGGGVGPYGGLHGLQLDALQSVRMVTGTGSLVNVSATSHPDLWWGMRGAGFNFGIVTSATYQVYTFTNNGQAMSADFRFHASQNASLYEFARSYTGKLPDAFSIDIAIAYNEAFGGTYLFANFIYAGPLEEGMALIQPIIELGPFDQNITMIPWKDIETSSKFGIDALACIKGNFHSVWGLNLYQIDVPTLIDAVSYMDTVYAQYPDFREAFLAIDMYASRVIESVPDDATAYPYRNAVARLWVTFFLTPPIPTKELTTVSPNLSLLDVGFPNSSFATAASQVGRDARSLFVPTSGASTSNIEVYVHYGHGDEGQLGWYTERKLPRLNALKNEYDPNKLFSHYNALEATYDVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHK3</accession>
    <name>A0A8H6CHK3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LAA1-like C-terminal TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000178</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEATR5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152553.1">
        <property type="nucleotide sequence ID" value="XM_037291118.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59328597"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040108">
        <property type="entry name" value="Laa1/Sip1/HEATR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046837">
        <property type="entry name" value="Laa1/Sip1/HEATR5-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057981">
        <property type="entry name" value="TPR_LAA1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663:SF0">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663">
        <property type="entry name" value="HYPOTHETICAL HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25468">
        <property type="entry name" value="HEAT_HEATR5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20210">
        <property type="entry name" value="Laa1_Sip1_HTR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25808">
        <property type="entry name" value="TPR_LAA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LAA1-like C-terminal TPR repeats" evidence="3">
        <location>
            <begin position="1861"/>
            <end position="2019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1250"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2011"/>
            <end position="2030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2042" mass="221278" checksum="69F6C34542EA3A84" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJD1</accession>
    <name>A0A8H6CJD1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010851</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153336.1">
        <property type="nucleotide sequence ID" value="XM_037301702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJD1"/>
    <dbReference type="GeneID" id="59339241"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="176" mass="19038" checksum="342B4FAB4F1F55A8" modified="2022-01-19" version="1">MTTKADAGCSRSAYAPKYIYPSLRPALRWSQSTNETIPSSPANATNAGLGAWPDTPFNRHLGWDTGIEIISCTPASPSDPTSEMGVLEGISIIGAKARSQSRLALIENFHEDSGPVTFDFHATEDLFRGSDVGKILDVLWEMTNQYGKGGVYGCLVVEDIHTAYFNLALKRVAVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJK5</accession>
    <name>A0A8H6CJK5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">DNA replication complex GINS protein PSF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009999</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The GINS complex plays an essential role in the initiation of DNA replication. Has a role in chromosome segregation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GINS2/PSF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153672.1">
        <property type="nucleotide sequence ID" value="XM_037300855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJK5"/>
    <dbReference type="GeneID" id="59338391"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11712">
        <property type="entry name" value="GINS_A_psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1020:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.50:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007257">
        <property type="entry name" value="GINS_Psf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056784">
        <property type="entry name" value="PSF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25005">
        <property type="entry name" value="PSF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028998">
        <property type="entry name" value="GINS_Psf2_subgr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF2 N-terminal" evidence="11">
        <location>
            <begin position="10"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="10">
        <location>
            <begin position="77"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="203"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR028998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="233" mass="25528" checksum="EF0762E0D44A272B" modified="2022-01-19" version="1">MALPLPPGLTPPETAFLCEMEMVTVIPRQRLEGLELLGGPIPPLNPPYRTSLPLWLALLLKRQRRANVLPPPWLSPPSLHSILDFEKSTPSFSPPLPLPPHTTTSPPFLPSATANAPPDALPYHWLELGEMLLEAASDDIPEPDNVRRLMRDLREVRMAKMRAGVGVLEGGREVKMNGVGGMEVAEGRAFIGGVVDGLRKIGASKEMSRREREAEERENGIGGGEDDEDDMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKU0</accession>
    <name>A0A8H6CKU0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153808.1">
        <property type="nucleotide sequence ID" value="XM_037300991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKU0"/>
    <dbReference type="GeneID" id="59338527"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="68" mass="7514" checksum="0B693D6C22ECD432" modified="2022-01-19" version="1">MAGKGDHQESRRAAKAAVGTGLAAMERKHSAERAKLEQERVEMLRLLDEDIAADEDGLRFEAKFEEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLA8</accession>
    <name>A0A8H6CLA8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010393</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154063.1">
        <property type="nucleotide sequence ID" value="XM_037301246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLA8"/>
    <dbReference type="GeneID" id="59338784"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF162">
        <property type="entry name" value="SATRATOXIN BIOSYNTHESIS SC1 CLUSTER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="33"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="283"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="304"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="451" mass="50038" checksum="F53FE5320992FE09" modified="2022-01-19" version="1">MSQAGNASQVPIDVNKGVTGALFGLAIIAFIIRSYICIRIHKKVHVEDFILLFAVMSLCAATGLAYATLTAQYALLQLVLHGFEGDIVFRLLGEIPSISKEENAATNLWWLVIFSVKMAFLFFFRRLISRLRSLNVWWWFVTALTIIAGLVSVAASWLTCPYFTIGGLLSCAGTSADHRTIRDTAITTVMDIMSDLLVLSLPVSILWKVRISVRQKIGLAFSLCLSCVMVVVTIVRVAGMRQRGSGSVDIVWLAFWQQQECSIAVLMCSVSAFRSLFVPSPARTPIPRQQRDSPSEKRRRFPHRRPDPDLYDTHETNGLPQIPSAKLAGIATEIRGGRQSEETASLDQRFNSHLPPSEHDHPTEIATTNSSARDAELGLSIESMLPHTCPETSNARQSKSSGTRWWKAFLQPETAPTASTTRTGYWEVISLFRTGHSESIVQSKDRTEGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLC0</accession>
    <name>A0A8H6CLC0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009643</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154352.1">
        <property type="nucleotide sequence ID" value="XM_037300504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLC0"/>
    <dbReference type="GeneID" id="59338038"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15502">
        <property type="entry name" value="PHD_Phf1p_Phf2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12628:SF10">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12628">
        <property type="entry name" value="POLYCOMB-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="346"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="144"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="312"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="553" mass="58925" checksum="25B3631796876F87" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLS3</accession>
    <name>A0A8H6CLS3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fungal N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154551.1">
        <property type="nucleotide sequence ID" value="XM_037300703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLS3"/>
    <dbReference type="GeneID" id="59338239"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039327">
        <property type="entry name" value="CON7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36167">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36167:SF3">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="55808" checksum="8D3898BD3C1BBC5D" modified="2022-01-19" version="1">MDGVSIAASIVGIATAGVQVSIKLVTLASQISTASDRISSIGNDISLTSGVLHQLGELINRKTTDDGISILNQDGLKTTKTSAAMCERIFQEVEKEMKRASEQLRRYKPGQGRMSGEKIELSMTEKAKWLFLQPSIDRWRADLRDAKSTLMLMLQVASLALSKRMADASMSITEHQDFVRAIVALELQRREERTTSFQQQKVLRSSSSDDTTKENADTTPAGEGTLNSDRNHQIPEPVVQAGEKRTAGVIKFRKLLSDLPRTDDTSDVPEYYTMDSSHISFSSDSDSGTQTNHNTELQLFLLKPIVEDLFDRIELRWSVENTNLRPLAIRQHLAENKKNDLPSVVEMLQHLHAYEQAMVDSETTKGSGGSVLSLERTKTDIQSRDMLFQAVPGLQFVVQRRVRQPPRLFSTPQFEYRHPSSSTPTQRSMSVNFHKGAMSRRRPTSTRRQPRPSTSKILGSRRAAVPTAASVILDERDQGDDEADAVVTGLLRKYTTLFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMU4</accession>
    <name>A0A8H6CMU4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154977.1">
        <property type="nucleotide sequence ID" value="XM_037300151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMU4"/>
    <dbReference type="GeneID" id="59337685"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="153" mass="16491" checksum="EB9AFEEEE7AEA1FB" modified="2022-01-19" version="1">MPAPVKATTTRSLPIDQSILLYGAVLVSASVTIPFWLYTDSMWASFTPKLNAGGGPTQEDKEIECFLAAVEEAESHNARLHSARRGQHHGDSDEDEYGSDSEDNDDDVDESDNETEGGSTLNITSCGPRNCIELDDESSGSDSEGSPARMLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNN5</accession>
    <name>A0A8H6CNN5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155183.1">
        <property type="nucleotide sequence ID" value="XM_037299182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNN5"/>
    <dbReference type="GeneID" id="59336712"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00251">
        <property type="entry name" value="Ribosomal_bL36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_bL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_bL36_sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052010">
        <property type="entry name" value="Ribosomal_LSU_bL36"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18804">
        <property type="entry name" value="RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18804:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="Ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00828">
        <property type="entry name" value="RIBOSOMAL_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="134" mass="14661" checksum="6F59BE4CE35088D8" modified="2022-01-19" version="1">MLQLRANAAMTALRNFSALPYLQHTSRTTSSRTSLLICASCTARAFSAGSGGSLGRSTHTGLLQGKWRSLAGLLGKLEKRPGLELVMHEKPEVSRGMKVRSSVKKLCDGCKSVRRKGYVYIICSKNPKHKQRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNP9</accession>
    <name>A0A8H6CNP9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SNF2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009437</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155124.1">
        <property type="nucleotide sequence ID" value="XM_037300298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNP9"/>
    <dbReference type="GeneID" id="59337832"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034292700" description="SNF2 N-terminal domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="SNF2 N-terminal" evidence="5">
        <location>
            <begin position="172"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="236"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="332" mass="34457" checksum="5B8D12BCE61AE0D2" modified="2022-01-19" version="1">MSPITFTLFIIYALSAGVLIASSPTPGPSIRAGVNITVFSDTACGHSPQNHSFSYGFMWPTPFTTQSYTLSRALSSEERLDWSNPYPAGGRAPNPGEIPQACGTYLQTTNPDSANPHSGSTLHAGTCYLMVGGAATEDIQRSCVVNAKFMTPKGYGPQGAKPLEMRARPHDYQLQGLQWLSLPNPVGKILANDMGLGKTLQALATAAALPNRPNGARQVLVVVPCQVLRNRFNETNKAGLPSLNRPPPGPPASPSVGSGEATSSSSTDNDGGSGDEHVSGASSARTPSGSPPPPSCGDSLNGHGPPPPSSEDEDEEIEDAAAATWAGGHETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP76</accession>
    <name>A0A8H6CP76_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155135.1">
        <property type="nucleotide sequence ID" value="XM_037299134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP76"/>
    <dbReference type="GeneID" id="59336664"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="17"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="168" mass="18254" checksum="A384CA7B7DABB88F" modified="2022-01-19" version="1">MASDRFHGIDGKTQTELLTIESCISHTFKNPVLLLEALQFPRSIVGWHGYDDNTGLQVVGSNAISLARAVVEYNYTGLANSRVLNETHNTMWLEDSVSVCGRKIGLLDLIERSGYGGDQNEATAILRLTIKALMGAVYIDGGMEAVVVVLDEYDDHLIRASLGREGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPD4</accession>
    <name>A0A8H6CPD4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155418.1">
        <property type="nucleotide sequence ID" value="XM_037299417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPD4"/>
    <dbReference type="GeneID" id="59336947"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="405" mass="46502" checksum="08814A7E9CD18915" modified="2022-01-19" version="1">MSHREGYHSDQWARPGHDREDLAPSRHPHEASSRSPHKPYPDDHGIAPFASRPVYTDAYSTASPSSLYGFSEERASLDPRAVGALQQIIPPYRDEIMDRNRVLAMPPPTRRPGSYEPPDRLDTLSQAAPQLSRIPTSFQHPYSHPEAGMGFNAVRSPPGLTRRMSCQDVNQRPPSSAGNNPWETEYTIPQDRAEYLDERVASREAAILPEPKGYERQPRRFRGGRDEFAEGQVQHLPKIQVQQARERDLDYEPISLDLQEQNDILSRVNRRLSQCAFDFIAMYQFPIPLEAGKPMVRVATDRAWTEWVYLLKRLATKRRIPSKAIYDGQIKELTTLLDNSVEMRHSTKPQSRPLKDDRHVLQLISAGIQVGRMLKDATTMDYLDRLYKDTEALVQQRKSMPRYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ19</accession>
    <name>A0A8H6CQ19_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008838</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155702.1">
        <property type="nucleotide sequence ID" value="XM_037299701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ19"/>
    <dbReference type="GeneID" id="59337233"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40619">
        <property type="entry name" value="FUNGAL STAND N-TERMINAL GOODBYE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40619:SF3">
        <property type="entry name" value="FUNGAL STAND N-TERMINAL GOODBYE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="3">
        <location>
            <begin position="112"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="423"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="490"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="510" mass="56896" checksum="E18DA6E8DAD9BA61" modified="2022-01-19" version="1">MKIREEIKREIDGLLQDFDTRWVQRFDELLLQQTALFDGRNDTGRPDLNDSNAQLALPVFTPQVYLADFVSDPQTLLEFRNEAFPQLQRFDQRESYIRAGSRLLTAYDWQHCVALLRHPSMRSWMSTEKSGILWVDTYQSHKLDWASVFSTRLADDCARLDYSMALAHFCQGHSTGNAITTAAILIQSLISKSISLHHEQFTVRTVELTQQHFQDAQNDVEELWALFLEILEVAAKGKCVWIVVDHVDILQQETNRKGLENALALLRNLNALADDPAITVKILIAARIRDAARLSTKIAEARILASRHAIITVPRGHHRNEATLLKSSKKISRLPEPNVSLNFLRASHTAKRKPETATAPKEVDDDTGESDSASLFDPFASSDDSEPTTRSKKAAQHSCSSEDSADEYFSQTRPFGDFAKEIGWESTDDEDHLRKQAISPLTPKIVVAFSEHSPRSGASRSGSDKDGVNAGANEMQAQPDLSTPKAADSGSMSQIHNSLPNDSDSDGAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQV1</accession>
    <name>A0A8H6CQV1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tudor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007678</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155884.1">
        <property type="nucleotide sequence ID" value="XM_037298548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQV1"/>
    <dbReference type="GeneID" id="59336075"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd20446">
        <property type="entry name" value="Tudor_SpSPF30-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041297">
        <property type="entry name" value="Crb2_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46297:SF2">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46297">
        <property type="entry name" value="ZINC FINGER CCCH-TYPE WITH G PATCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18115">
        <property type="entry name" value="Tudor_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="4">
        <location>
            <begin position="106"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="56"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="155"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6227950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="296" mass="31952" checksum="90D02E8A21EA7783" modified="2022-01-19" version="1">MSNEVASLEAEIKEYKLQLETVQSGLQADPDNADLQGLKIELEEVISLTESAIAELKPASAPAPTKKPSSPPIKEKWSKENHPAFQAGYRKPAAPQSPVEEPQAPTTFSVNDNILAKWKSGDKAFYPARITSITGSSSKPVYIVTFKNYTTTETLSAHDIKPISSDSKKRKADGPPGNPTTPSIPTSSNVISAAANIDPTLASQAKKEPSKVSDGPVRPAKVPRKVKANKELEAGKNKWQDFTTKGKTGKVAKKESMFRTPEGVNARVGFTGSGQQMRKDPGRSRHIYQQAGDEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRD3</accession>
    <name>A0A8H6CRD3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongation factor 1-alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ERF-3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">ERF2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic peptide chain release factor GTP-binding subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Polypeptide release factor 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Translation release factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_007649</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155855.1">
        <property type="nucleotide sequence ID" value="XM_037298519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRD3"/>
    <dbReference type="GeneID" id="59336046"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018444">
        <property type="term" value="C:translation release factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000017">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000503">
        <property type="entry name" value="Peptide chain release factor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000061">
        <property type="entry name" value="Translation release factor eRF3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003285">
        <property type="entry name" value="Sup35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01343">
        <property type="entry name" value="YEASTERF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Tr-type G" evidence="16">
        <location>
            <begin position="264"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="83"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="83"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="234"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6227921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="699" mass="75863" checksum="24B1C01E53D4F805" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRR1</accession>
    <name>A0A8H6CRR1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008073</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156277.1">
        <property type="nucleotide sequence ID" value="XM_037298941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRR1"/>
    <dbReference type="GeneID" id="59336470"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="73" mass="7793" checksum="93B5294154E956EE" modified="2022-01-19" version="1">MGVDCAEFWSGLRLRDVEASVIWQIIRLIVIALPPPESDAKSNADLAPICGFTTYTLKSAAAQDRGSDVTSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRX8</accession>
    <name>A0A8H6CRX8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA ligase/cyclic nucleotide phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156422.1">
        <property type="nucleotide sequence ID" value="XM_037299086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRX8"/>
    <dbReference type="GeneID" id="59336615"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="35714" checksum="8A7B82A56B5E8FB1" modified="2022-01-19" version="1">MSRIADGNVFADLSGTSNSTTENPYDALLEACKQEPLRYQTHRETRNDQQKSKLTSPDFSGFIIDEVLHKLVNPEINPGYVDPRNCLVFWARPPKRIRQLVETLQKRLLGYAPSLWLMPVGCLHMTVLEITHSKTEAEIEHLVNTMESKVPELTDFTYDHRARLVKPMLSYDAAAIALSFLPAAGEGLPAEKAANADAYTYHHLRRDMYGLCRSAGVEVASRYVVPSSHLTIARFVTQKDISSKDGNAVDRGKIEGLVRTLEEINGELRAKYWSEDSLTSEGSWTVGEEKGLDYRKGTLWYGGGHSHHEGKGFNFDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSQ8</accession>
    <name>A0A8H6CSQ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156445.1">
        <property type="nucleotide sequence ID" value="XM_037299109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSQ8"/>
    <dbReference type="GeneID" id="59336638"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35585">
        <property type="entry name" value="HHE DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_4G00730)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35585:SF1">
        <property type="entry name" value="HHE DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_4G00730)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="1">
        <location>
            <begin position="26"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="221" mass="25004" checksum="B5ADFC83E152C18E" modified="2022-01-19" version="1">MSALEPVVAKLAGDPAHDENQRTISEAIGADHGAIDVYSDNIKTASTLQEKIEWRNQFTWALARHAVSEELTLYPVMEKKLGQKGKEMADVDREQHMAVKKDLYELQDMSPDNKDFFPLLDILMTDLHKHIEHESNEDMPRLEDILTREESKSLANYFEKTKLILPTRSHPSAPNKPYFENFAAMLAAPIDKFMDLLRAFPEEGKLGDVSASEPPAVVQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6D7</accession>
    <name>A0A8H6F6D7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyrroline-5-carboxylate reductase dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000028">
        <property type="protein sequence ID" value="KAF6217430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146865.1">
        <property type="nucleotide sequence ID" value="XM_037297796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6D7"/>
    <dbReference type="GeneID" id="59335300"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="3">
        <location>
            <begin position="389"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="462" mass="48800" checksum="E4F23364ED443220" modified="2022-01-19" version="1">MVSLGVAENLPSRIETSMGQSRSLRNTALWYPEIPNPALYLNEDSFAGEDASSGRYQPLCWTLFGGPSGTFLRSLTEISVTFFGSLCGIEFHYNTEDVPVERRKLGRRKSTEYAQVMHFPIDGPGGEIIETVEVNFVDLGSLYGEPAKSFYGHEKLRLRYADPDLPTKLTPPTTLTLHPGKLSSAILQGILDSLTNSKPTDFRPPSPIPGTTDTQSPPSNPAIITPTSLTACVHRPSSAALLSAKFSHDPLVTVLADQNVLGAQRGDVVILGVEPHAYRPVLAAPGMAAALRGKTLVSVVGGVPAAKLYGAIYHGTGEEEEGEGEGEGRRCHVVRVTAGTSAAVRGSVSLIGEEPGRGHPPAALNAVYSLFMRVGQVKMWPEHLQAVGATMTAGGLAFLSVAVEGLVEGAVREGVERGEALQMAASCMVGLSKLIASGEAPGEVRNKVATPGGESSALRHDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7N6</accession>
    <name>A0A8H6F7N6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006526</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147134.1">
        <property type="nucleotide sequence ID" value="XM_037297424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7N6"/>
    <dbReference type="GeneID" id="59334927"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="266" mass="29397" checksum="E5C6DFFE6BA4841E" modified="2022-01-19" version="1">MASQFFKKMRSLFYQQSTEQEKANSFHTINGVFGDWATSAVEDVPPPYTDSPEAKTHFQDEKDILPPPFAGPCLQICPHEALSFEDLKNTISSTAIKNNGEIIDALTLGCHEPRSQFDLAAVDPKHVCVSSPSSLRGFGTYASHVGKEPARAPGIMLSFQWDLGWLDGVRAQVETAAELQQFLNADGIWLCPHKRINDSDIVNAIYGFVKRSLGREILTGCDCCDTEITILARSEGNDETCRVKTRRYLGAVETPDDPIWLAQCGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7W0</accession>
    <name>A0A8H6F7W0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147418.1">
        <property type="nucleotide sequence ID" value="XM_037297293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7W0"/>
    <dbReference type="GeneID" id="59334796"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="248" mass="26472" checksum="F9296836B9B44F2E" modified="2022-01-19" version="1">MSPQFPSKSAENLKVANVCGYCQRQGMLSLPAHHPGTNGSVTGPSAQSAANKLRDGIVHLTITQLEESKDDGHHPTSEDVARLWEELISAACERDCGCAQVLRRHVHCDELRSFWAATSVEHSEGEPLSRRQKKSKKKNAEKAEAKDDQPPAPLGKLRSENRRYKKTKARAKQAAVPTPTASTAQSSLPGQAAATGSSIYDIEGLQQALPSVLSAAPPAVSPANSGQTILGLPVTFVATLAVHQKMAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7X5</accession>
    <name>A0A8H6F7X5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147945.1">
        <property type="nucleotide sequence ID" value="XM_037296764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7X5"/>
    <dbReference type="GeneID" id="59334264"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="53615" checksum="0855734F04548061" modified="2022-01-19" version="1">MEEPNARPHTASDTAQLRLTSLTPELKCAVFANLPDVTSTKSLALVSSSFYYTFLDAQTLILSQVLQNEIPTDLMHGAFAAYNASRIPVWTKQAVQDFLDEYFGNSVPHKAQKWNLSEALRMSKFHSCVEFFATEFASSALSKNPSTRGSNAAPSFTEILRIKRTLYRFELYCNLFRKPSYHRMVRGVRNCLIQPRPFGASEQREIFFDLFSPWENEQLGCIYDYLVEEITVPFNDVAEHDVDWGELSIPWVDTFGSSEIWYKERYLLVGLDFIFQLSTASTYDDRHRLLKSDQVSGESFLSDAMIPPRLLQHDGVPLEEDSDEEKEPWSALLLMTTMIAVPPKHGDRFYFLEDHRRFRQRGYVMWDLTRLLEWEFFALPVQALAAERLPGYSGCLEQQAEMDRQKVSWKERSRIWGKGGTGWWAPGDESHIQWPRRYQNPQVPSTQKYTLALPKILKAAPKSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7Y0</accession>
    <name>A0A8H6F7Y0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147404.1">
        <property type="nucleotide sequence ID" value="XM_037297279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7Y0"/>
    <dbReference type="GeneID" id="59334782"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF13">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="241" mass="27623" checksum="58BF7C86714376EB" modified="2022-01-19" version="1">MAESAVGPKLEIVTALAALLGQLRDYKFDRVGNLYFKGRERRPDPEKQSKAASLAHSLSKGHSELHVSGWQWLQMSWVKTGPMFKASTNHADFDDDDWGSDCDGEDPDMEKLSSDYQDVLPWVFPAAKNVSPYVLYHHNMSLAKISVNPHTYEITGIINWETIQVVPERKSSRFPEFLTDQKDFEEIDDDEPRVLTPAEYDEDGDDYNATVVERKDRWDNGILRQHYDVAFKLGCLTSYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7Z1</accession>
    <name>A0A8H6F7Z1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147766.1">
        <property type="nucleotide sequence ID" value="XM_037296585.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59334083"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42105">
        <property type="entry name" value="DIM2-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42105:SF1">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="703"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="881"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1245"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1316"/>
            <end position="1757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="496"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="703"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="883"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1397"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1428"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1457"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1470"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1507"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1583"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1679"/>
            <end position="1697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1757" mass="193894" checksum="59BC0D87127272D3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9P2</accession>
    <name>A0A8H6F9P2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003502</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149805.1">
        <property type="nucleotide sequence ID" value="XM_037294424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9P2"/>
    <dbReference type="GeneID" id="59331913"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="85" mass="9400" checksum="4E7BD70C6A59780F" modified="2022-01-19" version="1">MFLRYEGANGGKYTSLVHTSLQVLDKSNTPSSGVSKTAKIVKNLMIANGLSLVPDKSWPPFGPLAYRLSYVENSQLEQRFADYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9Q7</accession>
    <name>A0A8H6F9Q7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002925</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149927.1">
        <property type="nucleotide sequence ID" value="XM_037293849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9Q7"/>
    <dbReference type="GeneID" id="59331337"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF194">
        <property type="entry name" value="INVERSIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="453"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="585"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="70367" checksum="3AC1B95878819476" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9V6</accession>
    <name>A0A8H6F9V6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HO133_003090</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:16638"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the C-terminal section; belongs to the chitin synthase family. Class V subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the N-terminal section; belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150092.1">
        <property type="nucleotide sequence ID" value="XM_037294014.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331502"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14879">
        <property type="entry name" value="MYSc_Myo17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000012">
        <property type="entry name" value="Chitin synthase ChsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000017">
        <property type="entry name" value="Chitin synthase ChsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000019">
        <property type="entry name" value="Chitin synthase ChsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000055">
        <property type="entry name" value="Chitin synthase ChsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036037">
        <property type="entry name" value="MYSc_Myo17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF45">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 18" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 18" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10 18" id="KW-0518">Myosin</keyword>
    <keyword evidence="7 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="913"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="952"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1218"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1616"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1644"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1671"/>
            <end position="1694"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="22">
        <location>
            <begin position="1"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="21">
        <location>
            <begin position="976"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="domain" description="DEK-C" evidence="23">
        <location>
            <begin position="1814"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="604"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1889"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1954"/>
            <end position="2023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="1959"/>
            <end position="1989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="2002"/>
            <end position="2013"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="102"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAF6220657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2085" mass="231943" checksum="85706BA9F43F90D4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB17</accession>
    <name>A0A8H6FB17_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002153</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <topology evidence="1 9">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150733.1">
        <property type="nucleotide sequence ID" value="XM_037293080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB17"/>
    <dbReference type="GeneID" id="59330567"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF5">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 9" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034905020" description="1,3-beta-glucanosyltransferase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="406"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="452" mass="47714" checksum="1436113222CE250B" modified="2022-01-19" version="1">MKGINAIALALTATTASASLVPHYFKRASSLASAAPPVLLTQLLTRAPAHETSPSKQKNYLMFEALLIRGNRFQKLKINTVRIYTVDNSQNHDACMSALATAGIYLALDVNTPKYSINQDDPAPSYNADYLQSVFSTIDAFHTYSNTLLFFSGNEDINSVATTSSAPYVKAVTRDMKNYISARKYRSIPVGYSAADVDTLIYQTADFMNCGDDAMARSDFFAYNDYSWCDPSSFEGSTWQAKATYFANFSIPLFLSEYGCVKTTRQFQEIATLYGPQMTPSYSGGLAYEYSKEGTAPPADLYGLVEINGNSITELTDFGTLASAFSNTPSPTGSGGYRPSGSPAICPTQDSNWNVTTGSELPVFPSAAATYLKNGAGTAPGLSGSGSQNSGPASVAFASGSSVAATGTSTSTSTSTSGSKKSAADGRVIDSAPVMACGLVVAMSTLLGMALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB89</accession>
    <name>A0A8H6FB89_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150869.1">
        <property type="nucleotide sequence ID" value="XM_037293216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB89"/>
    <dbReference type="GeneID" id="59330703"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="688"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="781" mass="86821" checksum="7A9FC400A96CE798" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBV0</accession>
    <name>A0A8H6FBV0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peptide hydrolase</fullName>
            <ecNumber evidence="15">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_000682</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in vacuolar sorting and osmoregulation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Vacuole membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151981.1">
        <property type="nucleotide sequence ID" value="XM_037291619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBV0"/>
    <dbReference type="GeneID" id="59329100"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03875">
        <property type="entry name" value="M28_Fxna_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000057">
        <property type="entry name" value="Vacuolar membrane protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048024">
        <property type="entry name" value="Fxna-like_M28_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053975">
        <property type="entry name" value="PFF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053976">
        <property type="entry name" value="PFF1_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF58">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22250">
        <property type="entry name" value="PFF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22251">
        <property type="entry name" value="PFF1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 15" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <keyword evidence="10 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="544"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="682"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="720"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="18">
        <location>
            <begin position="171"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane protease transmembrane" evidence="20">
        <location>
            <begin position="452"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane protease C-terminal" evidence="19">
        <location>
            <begin position="775"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="614"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF22251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6222635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="997" mass="109667" checksum="421CD5E593EF5AF8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBY0</accession>
    <name>A0A8H6FBY0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000788</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152086.1">
        <property type="nucleotide sequence ID" value="XM_037291724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBY0"/>
    <dbReference type="GeneID" id="59329206"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="99" mass="11214" checksum="EED8BC8BC1D26C26" modified="2022-01-19" version="1">MSLKKLNKVTFLEPERQTTEEHQGPSKSLKETLEVVQTIKTALRDVEHHVDQTAASEAGVKRQLEEQVMKTKQEVVRLERMLAEELEAAPDVFEAEEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCH4</accession>
    <name>A0A8H6FCH4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152497.1">
        <property type="nucleotide sequence ID" value="XM_037291062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCH4"/>
    <dbReference type="GeneID" id="59328541"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF150">
        <property type="entry name" value="OXIDOREDUCTASE TDA3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="417" mass="44156" checksum="BCFC68E80707D711" modified="2022-01-19" version="1">MTVNSEQRNIVIIGGGIIGCTSAYFLTRHPSYDPSKHKITLLEASKIAGGASGKAGGLLALWAYPSSLVPLSYRLHEELAASHSGAERWGYRKMHSGSLTAKGRTIGQQSAANGNHASGDSVSLQKLKDKVSRTLAAAGIPKDLGWVAADSARSYYEMGTPDNTAQVHPYQFTTAMAQLAEDAGAEFILGSVTSIDRTGGAVKSVTYNDKATSEPHTIAATDVIICAGPWTRSIYPPAPISALRAHSVTIRPSKPISAYALFTEISLPPNFGRTDTFQKSNRAHSKQVTPEIYARPNNEAYACGEGDTMVPLPKTTQDVVTDDSRCQDIIDYVSSISDELRDGEVTARQACYLPNVQAASGGPLIGETGVKGLLMAAGHSCWGIQNGPGTGKLISEFVFDGKAKSASIGSLDPRKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDP1</accession>
    <name>A0A8H6FDP1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF431 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010617</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153103.1">
        <property type="nucleotide sequence ID" value="XM_037301469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDP1"/>
    <dbReference type="GeneID" id="59339007"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18090">
        <property type="entry name" value="Arginine_MT_Sfm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007364">
        <property type="entry name" value="SFM1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35517">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE SFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35517:SF1">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE SFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04252">
        <property type="entry name" value="SFM1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="23593" checksum="FE04924930F0C090" modified="2022-01-19" version="1">MGSISGKKIRSYIVEHLDPELGPWSTLEYRSIAQDSAKAGVRFFLSSVPTVMSLPPELRDLESLTIEHRSVEKLFQEDKGRVCLLDPAATVELSPEDGTKFDVFLFGGILGDDPPRDRTSELRKKGFSGRRLGPIQMTTDTAVRVTRMVVEQKVPLKDIPYVDYPELHIDENESTEMPFRYVKDADGSPCMPEGMVDLIKADSEKGFGDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDQ8</accession>
    <name>A0A8H6FDQ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010662</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153148.1">
        <property type="nucleotide sequence ID" value="XM_037301514.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59339052"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06627">
        <property type="entry name" value="STKc_Cdc7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000176">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000583">
        <property type="entry name" value="MAP3K epsilon protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000246">
        <property type="entry name" value="Putative Serine-threonine kinase SepH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053235">
        <property type="entry name" value="Ser_Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361">
        <property type="entry name" value="MITOGEN-ACTIVATED KINASE KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361:SF433">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="64"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="347"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="465"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1216"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="391"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="486"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1230"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1253"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1308"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1358" mass="150948" checksum="BC684CB72520FB2D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE27</accession>
    <name>A0A8H6FE27_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_011025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153508.1">
        <property type="nucleotide sequence ID" value="XM_037301874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE27"/>
    <dbReference type="GeneID" id="59339415"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="49474" checksum="2D138D30D448A2A7" modified="2022-01-19" version="1">MTHFPQLPNEMISEFWGYFQEPRDVENFAIVSKDIYAIGRPFVEEHNKLKREYSIFETGPGTRASAPAFLLKDVLLRPRFALYVTHLSIGRFRTNGRTQMEMVMIIYHTQKTLFIEAIKIASFVPQNDIRRWIKKVRAGGEDPILALLSLLLPELATITLTSIADISNKFPETIQRIARAEDRTFLTHLTRVNLFIEFPIDDYFLDWLWLKIFTSLPLVQPIHVKDLIVTQDHDDLVNEGQRLMSGSSCITEVTFVKSGVGAKTLFQLLQSIKGLKEFTYVEPIQEFKKFEPFWMRAALLAHAKQSLESLRITSSQTEEFGLLGSFRGFPNLRKLETNVHFLSPRAPFDELADLLPASIEEIYLHTGDHRCCDSVSPLIETFKKAKSQLLPNLRVLKLTSEPEMGTEQGGKDLIKTLEKTCRNVGIELTVISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEH9</accession>
    <name>A0A8H6FEH9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010093</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153766.1">
        <property type="nucleotide sequence ID" value="XM_037300949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEH9"/>
    <dbReference type="GeneID" id="59338485"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12520">
        <property type="entry name" value="RRM1_MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12523">
        <property type="entry name" value="RRM2_MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12521">
        <property type="entry name" value="RRM3_MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12522">
        <property type="entry name" value="RRM4_MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000047">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000064">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000130">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000120">
        <property type="entry name" value="Negative regulator of differentiation 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034195">
        <property type="entry name" value="Mrn1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018835">
        <property type="entry name" value="RNA-binding_domain_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF8">
        <property type="entry name" value="RNA-BINDING PROTEIN MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10378">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="200"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="291"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="425"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="516"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="24"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="588"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="737"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="795"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="593"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="615"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="745"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="798"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="855" mass="92216" checksum="B7F9085B8EE80EA4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEM4</accession>
    <name>A0A8H6FEM4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153930.1">
        <property type="nucleotide sequence ID" value="XM_037301113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEM4"/>
    <dbReference type="GeneID" id="59338650"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="22264" checksum="495FA6F37E2BA6B7" modified="2022-01-19" version="1">MRAQEGNRPTRVELSNNITMNILPECILRILLTTFLVANSINASPINNALDLLSLAATTTPPSNLTLPIPILGDPYSCFDAYLFNDRRAKVSDCTRAAAFLPNRHEPGTFHRGDSDADPFALPYVETYHSCSIKVDLKFGRPDEGSWLSVNIAVKKIIEACMVGQYPIGQTGGVTTAGTEGRIAVTVGKTQAVLGLGTNETGLRGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEN2</accession>
    <name>A0A8H6FEN2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear speckle splicing regulatory protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010244</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153916.1">
        <property type="nucleotide sequence ID" value="XM_037301099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEN2"/>
    <dbReference type="GeneID" id="59338636"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053246">
        <property type="entry name" value="NS_splicing_regulatory_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018612">
        <property type="entry name" value="NSRP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47845">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47845:SF1">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09745">
        <property type="entry name" value="NSRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear speckle splicing regulatory protein 1 N-terminal" evidence="4">
        <location>
            <begin position="94"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="312"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="362" mass="39685" checksum="9752F16BA9F5FC62" modified="2022-01-19" version="1">MMSNLSYGLNITKKPPLGQRPPPAKRKTIFDDDSGPEDGPDDEDAPESISTLGGLQPSSKPKSSISTSTAPPSRPSKPSKISQYGDLSTNHSTNKRSKSAQALDPNIYDYDAVYDSLHAKPASSTSNADVEKKPKYMGNLLAAAEVRKRDQLRAKEKMLAKERELEGDEFADKEKFVTGAYKRQQVEMRRLEEEEVQKEKDAEERKRREGGGMKALYKNLLERDEMKHKEVMKAVEEGRGQEGQADGEEKEAKKGKSEAELAKEKGAVINEEGQVVDKRQLLSAGLNAVSAPKPKPPPDPRSRGSGPGGGRGGRESARERETRNFEEQLLGKHGADDSSDDGADRASKSRKLEDELLGLGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEY0</accession>
    <name>A0A8H6FEY0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Azaphilone pigments biosynthesis cluster protein L N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009847</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154554.1">
        <property type="nucleotide sequence ID" value="XM_037300706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEY0"/>
    <dbReference type="GeneID" id="59338242"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031348">
        <property type="entry name" value="PigL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17111">
        <property type="entry name" value="PigL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Azaphilone pigments biosynthesis cluster protein L N-terminal" evidence="5">
        <location>
            <begin position="298"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="525"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="558"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="602"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="635"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="668"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="788"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="848"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="228"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="848"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="883"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="893" mass="98445" checksum="F262115F675E56BB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEZ7</accession>
    <name>A0A8H6FEZ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nitrogen regulatory protein areA GATA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154375.1">
        <property type="nucleotide sequence ID" value="XM_037300527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEZ7"/>
    <dbReference type="GeneID" id="59338061"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="54993" checksum="B9CE8FB519C7794F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG49</accession>
    <name>A0A8H6FG49_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isoleucine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_008307</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155175.1">
        <property type="nucleotide sequence ID" value="XM_037299174.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336704"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000111">
        <property type="entry name" value="Mitochondrial isoleucyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050081">
        <property type="entry name" value="Ile-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="14">
        <location>
            <begin position="509"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="1209"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="283"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="463"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6226866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1483" mass="166568" checksum="0B61AEA98E4A6A15" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGU0</accession>
    <name>A0A8H6FGU0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008757</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155622.1">
        <property type="nucleotide sequence ID" value="XM_037299621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGU0"/>
    <dbReference type="GeneID" id="59337152"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="4">
        <location>
            <begin position="48"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6227314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="130" mass="14116" checksum="F8F3CFEEC7748CEA" modified="2022-01-19" version="1">MASSIPSASTNGFQNASSYDKHRPSYPPEAVTRFLERLQVNGVKRARIVDLAAGTGKFTELLVGRNEDFEILAVEPHNDMRKELEKKGLMGVKVLEGEASSMAGVESQSVDAVIAAQVRPLTSTTRPTWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHD9</accession>
    <name>A0A8H6FHD9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Piwi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155818.1">
        <property type="nucleotide sequence ID" value="XM_037298482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHD9"/>
    <dbReference type="GeneID" id="59336009"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="1">
        <location>
            <begin position="582"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="925" mass="102159" checksum="7E85AAA4B136104E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHJ7</accession>
    <name>A0A8H6FHJ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="13">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_008111</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 13">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="13"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156315.1">
        <property type="nucleotide sequence ID" value="XM_037298979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHJ7"/>
    <dbReference type="GeneID" id="59336508"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF57">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 12 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="14">
        <location>
            <begin position="38"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6228381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="517" mass="57191" checksum="2D97775A18A65CBF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHM6</accession>
    <name>A0A8H6FHM6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamate-5-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008201</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156405.1">
        <property type="nucleotide sequence ID" value="XM_037299069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHM6"/>
    <dbReference type="GeneID" id="59336598"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000006">
        <property type="entry name" value="Gamma-glutamyl phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6228471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="404" mass="43044" checksum="256DE7A9BB710C66" modified="2022-01-19" version="1">MSLTDSSPLEVAKAASISSRNLAILSTEARNDALTAIHGALQETKDIILAANAKDLELATKAAEDGELSQSVLKRLDLGRKGKWEDMLQGVLDVRDLEDPGGKETTLSFRAISTTISNALTNTLVPNASIQLVTARDVIASLLDLDKHIDLVIPRGSNELVRYIKDNTRIPVLGHADGICSTYIHKDADPEMAVKILVDAKLGYPAACNAVETLLVDEAALSTILPAVASALLEKGVSLRCDAESKSALAASLPKNESTILQDATEEDYTTEFLAPILAIKTIPTPPDASASTSCSLAIAHINAHSSKHTDAILTSSPEIANQFQSGVDSACVFWNTSTRMADGMRFGFGTEVGISTNKIHARGPVGLEGLMIYKYRINGCGQVAGDYGEGGKRWRHERLPLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHV6</accession>
    <name>A0A8H6FHV6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sphingoid long-chain base transporter RSB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008228</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156432.1">
        <property type="nucleotide sequence ID" value="XM_037299096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHV6"/>
    <dbReference type="GeneID" id="59336625"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF8">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G14140)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="35972" checksum="B00EA9238DDF355A" modified="2022-01-19" version="1">MSNPPQPTILPATKDSVQNCTNVSEYCPVDLTIYGYYPNLGANAFFCAFFALCLGIQLVLGIRYKTWTYLVALGFGCLGEAIGYVGRIMLHSNPWSSNGFEIQICCLIISPAFITAGVYLTLKHIALELGANFSRIRPKFYTWIFCLADLFSLIIQGAGGGIAATANNGSSLQNVGNDMMMAGIVWQVFTLLIFGGMVGDYASRVYARRSELEHSAVKLMNATRFKLFIGGLVLAYLTIITRCVFRIGEMANGWGNPIMQDQGDFIGLDSVMITIATFCLTAFHPALVFPEMQMHGTTSPMGEFTTADAEKVHDAESPPNGDRKGYFGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHX5</accession>
    <name>A0A8H6FHX5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156503.1">
        <property type="nucleotide sequence ID" value="XM_037297868.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59335372"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="1321"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="714"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="803"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1084"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1170"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1411"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="573"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="601"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="715"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="815"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1087"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1106"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1170"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1246"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1411"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1449"/>
            <end position="1459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1459" mass="157687" checksum="E6863DB5E2B366E9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI83</accession>
    <name>A0A8H6FI83_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007107</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156635.1">
        <property type="nucleotide sequence ID" value="XM_037298000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI83"/>
    <dbReference type="GeneID" id="59335507"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF23">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="317" mass="33758" checksum="863A0842708B17DB" modified="2022-01-19" version="1">MLLYPVRWGPDAKLQDEYIYGLIVPVLPSWLPTRTGIPQEEVQFWTSAMLAAFGFANLLGSPFLGWLANRSPSRKTFLLLGLLLLGAATVMLSLGTDIYVLVVSWFFQDLSAAIIYTGGLALVKDTVGLDEIGKWMGFVLSFANAGFLILPTLGGLIYGKLGANAIFLTMGINVMLDIILRLGMVEKAGPRSNHLPENGDRQPIALLCVLLTVLGFAFSLTIPPLAADLASAVEHIIGENPEIGGEAGAYGQAFYLLNCGIPAGVLAGPSPAGFFHDTFGWRVMGWTLAVLSASAVLPIAAFTGNQESGSREDLNVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FID2</accession>
    <name>A0A8H6FID2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156761.1">
        <property type="nucleotide sequence ID" value="XM_037298126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FID2"/>
    <dbReference type="GeneID" id="59335633"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006886">
        <property type="entry name" value="RNA_pol_III_Rpc5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES III 80 KDA POLYPEPTIDE RNA POLYMERASE III SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04801">
        <property type="entry name" value="RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="567" mass="64218" checksum="3B0E2940A74ADB86" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIY2</accession>
    <name>A0A8H6FIY2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157014.1">
        <property type="nucleotide sequence ID" value="XM_037298379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIY2"/>
    <dbReference type="GeneID" id="59335887"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43040">
        <property type="entry name" value="RIBONUCLEASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43040:SF1">
        <property type="entry name" value="RIBONUCLEASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="1">
        <location>
            <begin position="6"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="284" mass="31082" checksum="34C1D21F3F93AD57" modified="2022-01-19" version="1">MASSIVDSPATIASLLDTLAALPTSPPSLYLDLEGTNLSRHGSISIIELLVLPQNHVYLIDVHVLGEKAFSTQSSDGRTLKVVLESPDIPKVFFDVRNDSDALYSHFNISLAGVQDIQLLENAARSFNRRCVNGLAKCIEKDATMTYPQRSDWKTVKEQGRKLFAPEQGGSYEVFNTRPLAEEIRKYCIQDVLFLPGLWSTYSAKVSAAWAEKLQWTGEAYGVGSMALKPSLMVTMAKDEAIELGKDVGLLAGIKASDLQQNVRAAPLMIAVIVDTVRLEPPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ03</accession>
    <name>A0A8H6FJ03_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007502</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037298393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ03"/>
    <dbReference type="GeneID" id="59335901"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="92" mass="10020" checksum="0158D9C030807AD6" modified="2022-01-19" version="1">MVAPLIRQLIEEEGAGEERGGVGYAEIEFDSPTLGDVAGRRQEAQLQTRLTNVEEMKDKDFLRLWIEDEAIRGESGGKGGGLFGGLFGLPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJV8</accession>
    <name>A0A8H6FJV8_9LECA</name>
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        <recommendedName>
            <fullName evidence="3">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">HO133_004201</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037295120.1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ARID_dom"/>
    </dbReference>
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        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF12">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
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    </dbReference>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
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        <location>
            <begin position="600"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="3">
        <location>
            <begin position="762"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6229864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="963" mass="107543" checksum="F1BE45D8670D22E4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJY4</accession>
    <name>A0A8H6FJY4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_004202</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
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            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157122.1">
        <property type="nucleotide sequence ID" value="XM_037295121.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59332611"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054295">
        <property type="entry name" value="CHS4-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF16">
        <property type="entry name" value="CHITIN SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22997">
        <property type="entry name" value="CHS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="496"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1072"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1097"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1123"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase 4-like" evidence="12">
        <location>
            <begin position="404"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="124"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="538"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1192"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="600"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1197"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1215"/>
            <end position="1224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6229865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1231" mass="138780" checksum="39D067F0D97BB7E4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL05</accession>
    <name>A0A8H6FL05_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamyl-tRNA amidotransferase complex subunit Gta3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004812</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157726.1">
        <property type="nucleotide sequence ID" value="XM_037295725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL05"/>
    <dbReference type="GeneID" id="59333218"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049545">
        <property type="entry name" value="Gta3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20978">
        <property type="entry name" value="Gta3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033994360" description="Glutamyl-tRNA amidotransferase complex subunit Gta3 domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl-tRNA amidotransferase complex subunit Gta3" evidence="3">
        <location>
            <begin position="657"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="791" mass="86251" checksum="FB861673C462DECD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FLK9</accession>
    <name>A0A8H6FLK9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7924 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000023</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157837.1">
        <property type="nucleotide sequence ID" value="XM_037290963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FLK9"/>
    <dbReference type="GeneID" id="59328442"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057684">
        <property type="entry name" value="DUF7924"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25545">
        <property type="entry name" value="DUF7924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7924" evidence="2">
        <location>
            <begin position="363"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="540" mass="59827" checksum="4DEC861F3CE15411" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYM2</accession>
    <name>A0A8H6KYM2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYM2"/>
    <dbReference type="GeneID" id="59335774"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027915">
        <property type="entry name" value="DUF4452"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39615:SF1">
        <property type="entry name" value="YALI0E17897P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14618">
        <property type="entry name" value="DUF4452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="200" mass="21929" checksum="92CFCA235C4DAD8D" modified="2022-01-19" version="1">MSAYYYGPQHHQPSSSSSHSSSSHNHHGGRSRRAPRLSASQNSHRPFRGVRSMKELTESPPTSAFRVRFEAGRSFDLDDDLEFCPGLLTEDDLQSIHSSSSDGSLSSGSPHSSPLQPQIQPQQQATPSFSLSSAASNNSYNPITGFQSNNSQQMKIHQPSALRTRNAIPIVNPSTGMRVPSPPSSISPAMMQQQTYVRHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZG9</accession>
    <name>A0A8H6KZG9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157145.1">
        <property type="nucleotide sequence ID" value="XM_037295144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZG9"/>
    <dbReference type="GeneID" id="59332634"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034150871" evidence="1">
        <location>
            <begin position="25"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="182" mass="19955" checksum="2ABF39CD1AA2D659" modified="2022-01-19" version="1">MTPKRLETLCLTIYIQLLWTCAVAAPAPLLKASPLASEGFGAGRNLLSPLPDSYNTTSPPPPSLTLLRSPLHLLFYDPTPPIPKAEAETLCNEALNEAGRNIARKFGDLHMRAEEYYAQGHVELVVEPGEEMTWLMEWEVPGALRDWLQGSGFRGTRFWVLWEGVEVGRGRIVGGQGGLDGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZI5</accession>
    <name>A0A8H6KZI5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004289</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CKS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157208.1">
        <property type="nucleotide sequence ID" value="XM_037295207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZI5"/>
    <dbReference type="GeneID" id="59332697"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000002">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00945">
        <property type="entry name" value="CKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6229951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="115" mass="13906" checksum="48A38CF1B9AAE1E3" modified="2022-01-19" version="1">MAALDHDRRNKQPRLLTEAERDRLEEFVEAIHYSSRYSDDENEYRHVQLPKDMIKKIPKDYFDPSKGTLKLLWEEEWRGLGITQSLGWEHYEVHEPEPHILLFKRPINYQPLVPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZN3</accession>
    <name>A0A8H6KZN3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed DNA polymerase theta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004454</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157372.1">
        <property type="nucleotide sequence ID" value="XM_037295371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZN3"/>
    <dbReference type="GeneID" id="59332862"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18026">
        <property type="entry name" value="DEXHc_POLQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.20:FF:000005">
        <property type="entry name" value="DNA-directed DNA polymerase theta, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000968">
        <property type="entry name" value="Helicase and polymerase-containing protein TEBICHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057220">
        <property type="entry name" value="DUF7898"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046931">
        <property type="entry name" value="HTH_61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048960">
        <property type="entry name" value="POLQ-like_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF6">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25453">
        <property type="entry name" value="DUF7898"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20470">
        <property type="entry name" value="HTH_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21099">
        <property type="entry name" value="POLQ_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="149"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="401"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6230115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="931" mass="102847" checksum="4AF73C45AE6A189F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZR2</accession>
    <name>A0A8H6KZR2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157389.1">
        <property type="nucleotide sequence ID" value="XM_037295388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZR2"/>
    <dbReference type="GeneID" id="59332879"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF2">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="26"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="236" mass="26808" checksum="85C2E594F70ADE5F" modified="2022-01-19" version="1">MAPYTADKYDPKTPKIFAHKPTMDAFDSIVLIKVGPGKQVFHIHKKLLCDNSTYFHAAINGSFKEAKDQSIEMPEDDPGVFRHFQYWTYTGNIVPEPQGAQEAKTIQWNILTSLYAFAEARDIPLLQNAAIDLAILRVQAEHRMPVDLLLDIYENTSENSRLRKFLVDWAAHKGDLKKKGWFGGRCPIEFFIDLAVAQHARTSGSVSEIANFWESRANYHVEVPVALSNKEKSEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6I4</accession>
    <name>A0A8H6C6I4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006566</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147174.1">
        <property type="nucleotide sequence ID" value="XM_037297464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6I4"/>
    <dbReference type="GeneID" id="59334967"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF16">
        <property type="entry name" value="LYSOCARDIOLIPIN ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="5">
        <location>
            <begin position="134"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6217739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="406" mass="46961" checksum="13E4E691F603F1D2" modified="2022-01-19" version="1">MPAKSVHQNGLATTNGSTVPILPQVKEAILQRVPVPEPTLPTRILRVVILLVYFWTSSIIIGTTQYIGTPLYLINKDHYYRFMAFTKQCMTLLVLSMTQWWTTTTIHVSGDESVQDQIHRLPDGHLQCNFASRMVLISNHQLYNDWIYLWWTAYINRPFNHGHVYIVLKESIMYIPLVGLGLRLAGFVFMSRNMKTDTPRLTRRLGQLTRRSNGGKGALQPMWLMMFPEGTNLSKNGRANSVKWAERQGERDLKYTLIPRSTGTYFCLKALNGTLEWVYDCTLAYDGVPEGQYGQDIFTLRSTYFERHPQAPIHMHWRRFKVSEIPLETLEDFHAWLIERWNEKETLLQEHAKTGQFPSSINQGKSITTEVKLDRWWEIWQLGAVLAPLLAGVFAVSRIFGQVRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6M8</accession>
    <name>A0A8H6C6M8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147051.1">
        <property type="nucleotide sequence ID" value="XM_037297615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6M8"/>
    <dbReference type="GeneID" id="59335119"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="419" mass="47387" checksum="5F3675983B8B356A" modified="2022-01-19" version="1">MGQPSLPQWRPRRTLIDLYNEKDQTLWYWRAAATLSALLIMIGFLIFPSAFKQNASLTISSKSATITAIILLVLGYVLSFVTALICRSWIFQLDIIFVPCLSSSVLGLINILYSLSTHEKSLQWTSPSIVALVLSTISTLTYTTIALLTFRKIHIVRARDAMHRYHSESTDAMMPESELQRQQLLRLLLQQEDAKQQNPDNGQSTFKIDWPGNADRRSTVVTLRNLPRAVRSSYGNRNSDLYGDQDANPPPLPPMDSVTEEASVDPRFGSSDGLLPLGERTYIPSNNSHIDGDIPGIVNTRYDSPIRSSLSNLPQLQANGYPIEKPEVQDLIVQHPTQRPEYHIVDEHARGRDQYRMVNQQGQQSNDAPRSRSRESRRIEIELADRGKARDGGRRRQELEGVEVIGSIRRVETDGWGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Q3</accession>
    <name>A0A8H6C6Q3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147402.1">
        <property type="nucleotide sequence ID" value="XM_037297277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Q3"/>
    <dbReference type="GeneID" id="59334780"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="197" mass="21757" checksum="DC7451797C4D9FCE" modified="2022-01-19" version="1">MLHVATSAATAGGQHGNEMHPPQARSFPAPTPFGTLIIIPIELRLKIYRLLIERGQSAVLRTSRATNEEAFDKSCEHAVCRLAFNCYLPFDICPSRVPWGIIQNLELRVQTVKYHDTSCIGYLNTWLPRFSASMTNLKTCKIRLDFGPAYHYEPEIITAIKTLVALQKMTLAIAVNADQDAHNDDGLHGSIIAGSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6S3</accession>
    <name>A0A8H6C6S3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006359</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147382.1">
        <property type="nucleotide sequence ID" value="XM_037297257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6S3"/>
    <dbReference type="GeneID" id="59334760"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="7">
        <location>
            <begin position="208"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="364"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="452"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6217947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="496" mass="55528" checksum="99A58B36022A881B" modified="2022-01-19" version="1">MATASAMIDFSNLPPLPEYHLTPLPSLLPPIPDKLLTLLLPIFAYWGLSMFFHWIDTKDYFSQYRLHTPAEVSKRNRVSRWEVIRDVLIQQVVQTAVGWVLGMTEPDDFFGKEQYDVAVWARRIRIAEKSIPRLLATVGLDARGIAKNLGASHPMLAGALQGGQYPSLENLSVVGSASKMGTPGFAGWELMLASAIYWVLVPALQFGLAILVVDTWQYFLHRAMHMNKWLYTTFHSRHHRLYVPYAYGALYNHPFEGFLLDTLGASIAYKLAGMNTRQGMVFFAGSTIKTVDDHCGYALPWDPLQHITSNNAGYHDVHHQSWGIKTNFSQPFFTFWDRLLGTVWTGGDVSARYERSRLAAQKAFDTDKEKTTVTHSNPADSGTQDPKPYENDLRDATDLPSIPIKNRQPEPPAGKAELQAAGSRQQILDDKKDGGPGVLVDEAYEEKEARGFLRRSPRKRAPSTLSSKDGFKGLRDRVPTSMHGRARGIIGVDGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C728</accession>
    <name>A0A8H6C728_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL6 alpha-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006653</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147261.1">
        <property type="nucleotide sequence ID" value="XM_037297551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C728"/>
    <dbReference type="GeneID" id="59335054"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000003">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="97"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6217826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="193" mass="21890" checksum="6BAB478DD6E3A02E" modified="2022-01-19" version="1">MRYIHSEETLNIPANVKVQIRSRIVSVDGPRGKLVKDMSHLSVCFSHPSRNIITIELHHGARKNVATLRTVKSLINNLIIGVTKGFKYKMRYVYAHFPINVNIEKNSETGLYDVEIRNFIGEKIIRRVTMLPGVDVEASKNQKDELQLTGNSLENVSQSAADIQQICRVKNKDIRKFLDGLYVSERGNIEDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7A5</accession>
    <name>A0A8H6C7A5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006118</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147594.1">
        <property type="nucleotide sequence ID" value="XM_037297020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7A5"/>
    <dbReference type="GeneID" id="59334522"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF16">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="4">
        <location>
            <begin position="203"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="802"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="837"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="873"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="571"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="579"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="601"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="967" mass="108100" checksum="8824F65CE0E951C2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7N3</accession>
    <name>A0A8H6C7N3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147589.1">
        <property type="nucleotide sequence ID" value="XM_037297015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7N3"/>
    <dbReference type="GeneID" id="59334517"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="394" mass="45608" checksum="5045E8968C8339B2" modified="2022-01-19" version="1">MGCAQSRPSANYYDEYFRPAQPPHRRYRSHRIANVDGYKPGPDYREHRQQYEQDVAADRYEDAYDRLTARSKLSTAHGLQEYYEDRGCHVKWWAQQKSACGYQAGLAAPGRIKAILPFKRHTLQLITTNHPPIHHAHNPIQQPHNPPPKMGATLSCLANDVSIADHPHEAPLPARPVSQQQLSRRQIQVPRRQPLQLEYSSEHGDEVPSYRRQVAQRPRRESLPVDYVDGHEEDVARFPGVRSQRQRQRPQRLRLDYQYGHDEEIPRLPYRSSPQRGALQREASYDEDGDDEGEASVSPPQFPPHQLPRRSSLPPPHSYSYEYDSDILRHERRPRRQSTRPRYEIEALPAPVYGDGLPERRMRRDQGLRGRESGSGDAPPSYEDHVFDREVGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C846</accession>
    <name>A0A8H6C846_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Multiprotein-bridging factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005862</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional coactivator that stimulates GCN4-dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters. Involved in induction of the ribosome quality control (RQC) pathway; a pathway that degrades nascent peptide chains during problematic translation. Required to prevent stalled ribosomes from frameshifting.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MBF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147948.1">
        <property type="nucleotide sequence ID" value="XM_037296767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C846"/>
    <dbReference type="GeneID" id="59334267"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013729">
        <property type="entry name" value="MBF1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245:SF15">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08523">
        <property type="entry name" value="MBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Multiprotein bridging factor 1 N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="150" mass="16008" checksum="08C1B7362F56657A" modified="2022-01-19" version="1">MSDDWDTVTKIGSKTGGGSSQRETVVRGKGAINQAQRSGAVIGTEKKFATGNPANKSGSEGQRLTKVDRADDIIAPNKVPAEIGQNIRNRRSKEGPYKMTQAQLAQKVNAPAKDIQTLESGDAIKNQALLNKVARVLKISPKTGLPLDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8V9</accession>
    <name>A0A8H6C8V9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Translation initiation factor IF-2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_004975</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148585.1">
        <property type="nucleotide sequence ID" value="XM_037295887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8V9"/>
    <dbReference type="GeneID" id="59333381"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03692">
        <property type="entry name" value="mtIF2_IVc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000007">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000008">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000019">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF20">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="16">
        <location>
            <begin position="293"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Tr-type G" evidence="17">
        <location>
            <begin position="728"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="41"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="103"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="201"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="443"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1019"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="452"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="471"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="550"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1019"/>
            <end position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6219150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1279" mass="141305" checksum="3392A6C7E68CFCFE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C918</accession>
    <name>A0A8H6C918_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fucose-specific lectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004992</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148602.1">
        <property type="nucleotide sequence ID" value="XM_037295904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C918"/>
    <dbReference type="GeneID" id="59333398"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.70">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89372">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="61800" checksum="B01AAFDD210E441E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C989</accession>
    <name>A0A8H6C989_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148670.1">
        <property type="nucleotide sequence ID" value="XM_037295972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C989"/>
    <dbReference type="GeneID" id="59333466"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013240">
        <property type="entry name" value="DNA-dir_RNA_pol1_su_RPA34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053263">
        <property type="entry name" value="Euk_RPA34_RNAP_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28155">
        <property type="entry name" value="ACR243WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28155:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA34.5-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08208">
        <property type="entry name" value="RNA_polI_A34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="647" mass="70223" checksum="A36948E3860ABE8B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9K2</accession>
    <name>A0A8H6C9K2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004926</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148536.1">
        <property type="nucleotide sequence ID" value="XM_037295838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9K2"/>
    <dbReference type="GeneID" id="59333332"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="186"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="353" mass="39968" checksum="F3EB77F8AC66FD3D" modified="2022-01-19" version="1">MAAETSSIPVVDFAAWSSDASLEQRKTIAHKLIEACQNVGFVYITNHHVSPERLAQAFEWSKKLFDLKLEQKMLAPHPSGFTVHRGYSWPGLEKVSNVMGDEDNTADLRKNLRQISDVKESYEIGSEDNKDQPNQWLPEDVLPGFREFMLNFYWECHKTAMSMMSAMALGVGLDDENHFVPAHPGHNNQLRLLHYPPVPAANIESQTSTRMGAHSDWGSITMLFQDDCGGLQIENPNKSGEFIDVPPLKDAVVMNVGDLMMRWSNDTLKSSVHRVTLPPKQDHFTGDERMTRARYSIPYFVGPEGPTIVECLPSCIDESHPVKYEPIRWNDYMLMRASMQYEAPPEAAAVRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA62</accession>
    <name>A0A8H6CA62_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148998.1">
        <property type="nucleotide sequence ID" value="XM_037294951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA62"/>
    <dbReference type="GeneID" id="59332441"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="201" mass="23375" checksum="31216BCAEF200A92" modified="2022-01-19" version="1">MAPKPYRVHVHQHAGSTAQEIQDKPEWSNAHQHRIGYCNRHDRRPGLTHTGDEEEEDFEEQAEDDRQELRRKVQNGDLVYFKDIVKREEVHLFASDKAILTTQKVINEVRVGFHLGRPRDHPTGWSYVMLFLLSVLDEWKHDSKVEANAKHNDAYATDETDQDGEKPQANGHTKESNTKYQKLRGKYSPQEIALLCSLQHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAF1</accession>
    <name>A0A8H6CAF1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tripeptidyl-peptidase II</fullName>
            <ecNumber evidence="4">3.4.14.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_004123</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Secreted tripeptidyl-peptidase which degrades proteins at acidic pHs and is involved in virulence.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal tripeptide from a polypeptide.</text>
            <dbReference type="EC" id="3.4.14.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.10" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149089.1">
        <property type="nucleotide sequence ID" value="XM_037295042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAF1"/>
    <dbReference type="GeneID" id="59332532"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
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    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
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    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000015">
        <property type="entry name" value="Tripeptidyl peptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF34">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE SED4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0843">Virulence</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033994542" description="tripeptidyl-peptidase II" evidence="16">
        <location>
            <begin position="23"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="17">
        <location>
            <begin position="213"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="508"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="550"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="551"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="586"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="588"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6219654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="608" mass="64368" checksum="BD6D17A88214F8D5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAJ8</accession>
    <name>A0A8H6CAJ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_003690</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 14"/>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149300.1">
        <property type="nucleotide sequence ID" value="XM_037294612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAJ8"/>
    <dbReference type="GeneID" id="59332101"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23284">
        <property type="entry name" value="beta-trefoil_MIR_PMT2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000012">
        <property type="entry name" value="Protein O-mannosyl-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF46">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="605"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="647"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="673"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="339"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="405"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="469"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="434"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6219865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="748" mass="85753" checksum="ED1830DFBD2617C0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAQ6</accession>
    <name>A0A8H6CAQ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-ribulosamine 3-kinase</fullName>
            <ecNumber evidence="1">2.7.1.172</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003629</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>N(6)-D-ribulosyl-L-lysyl-[protein] + ATP = N(6)-(3-O-phospho-D-ribulosyl)-L-lysyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48432"/>
            <dbReference type="Rhea" id="RHEA-COMP:12103"/>
            <dbReference type="Rhea" id="RHEA-COMP:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:90418"/>
            <dbReference type="ChEBI" id="CHEBI:90420"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.172"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:48433"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.172" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149239.1">
        <property type="nucleotide sequence ID" value="XM_037294551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAQ6"/>
    <dbReference type="GeneID" id="59332040"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102193">
        <property type="term" value="F:protein-ribulosamine 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1200.10:FF:000018">
        <property type="entry name" value="Fructosamine-3-kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016477">
        <property type="entry name" value="Fructo-/Ketosamine-3-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12149">
        <property type="entry name" value="FRUCTOSAMINE 3 KINASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12149:SF8">
        <property type="entry name" value="PROTEIN-RIBULOSAMINE 3-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03881">
        <property type="entry name" value="Fructosamin_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="486" mass="53625" checksum="A8B32C3DA0FEDE31" modified="2022-01-19" version="1">MKIDSAVIKALSLDPVRTTVARHGGSGFSTTAKITTTLKDGTEKHFFMKTGKGKDAEVMFAGEHASLNAIHMVPSLCPASFAHGPLEDAAGGAFLVTDFLDMSGTSLGSEKGSGMSLAAKLAKLHTTAAPIPEGFQKPVFGFPVPTCCGDTEQPNDYTESWADFYAQHRLLAILKKSERTNGKDPTLRSLVEKTVAETVPRLIGDKHLNSGKGINPVVVHGDLWSGNKGRGRIGGKGAVEDVVFDPSACYAHSEYELGIMRMFGGFGGSFLKEYHQICPKTEPVDEYEDRVNLYELYHHLNHHAIFGGGYKSGAVSIMRALHRKFSGKTLADQRLLRNLARAKRVLESYAHQLLYFLSQIEEPIFIDLTGAWDPSEQHWRDFIPSPADQNLLNLRKDQGAVGYLFHIDIPRSSLPLHAPAISIVRQSVNPGQREIYNMMNHSAKRLLGVVTQQKATSHGINHVWRIEKEDEGKREEVVRFAGWTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBU1</accession>
    <name>A0A8H6CBU1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peroxisomal ATPase PEX6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Peroxin-6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_003478</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with PEX1; forming the PEX1-PEX6 AAA ATPase complex, which is composed of a heterohexamer formed by a trimer of PEX1-PEX6 dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Peroxisome membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149781.1">
        <property type="nucleotide sequence ID" value="XM_037294400.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331889"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19527">
        <property type="entry name" value="RecA-like_PEX6_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000109">
        <property type="entry name" value="Peroxisomal biogenesis factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000039">
        <property type="entry name" value="peroxisome biogenesis factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056995">
        <property type="entry name" value="PEX6_4th_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047533">
        <property type="entry name" value="RecA-like_PEX6_r2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF9">
        <property type="entry name" value="PEROXISOMAL ATPASE PEX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23315">
        <property type="entry name" value="PEX6_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23120">
        <property type="entry name" value="PEX6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="1062"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="304"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1324"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="331"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1387"/>
            <end position="1396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6220346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1430" mass="154306" checksum="CC5D497FA8277F8E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBV5</accession>
    <name>A0A8H6CBV5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149913.1">
        <property type="nucleotide sequence ID" value="XM_037293835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBV5"/>
    <dbReference type="GeneID" id="59331322"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="457" mass="52347" checksum="15AF6481DBE2413B" modified="2022-01-19" version="1">METPPFLRLAAETRDQIYAYLLSTKYTKHYPREEDPEWEYNYDLTKDFDCTPRPTHVYRFHPGILGANRQINREASCVLYDQNMFVEVTSDLTDWNVDFDWLGVPIVAEGAIASGFVYSVLHIKVELEHLIHYQDKHPFQRIFAGADLPAFCTALLLGGESSKAIYESTLSVNLHCNTTAETSPNRASFTIPQVKSLLEPLRILHSFQSTNITGSLDEQYKTELCLDICKKAPSLQERSFKVRDKIREGDDAFNARNTKPKDRFSQAIRLYEAAMTDLKNSCSRFDQPVRVLAANPISFSTAGSDFHHVYTNIVFSLRIKLAAAHAQLEQYEQCRKWSGRAVRTIDQDGGAPDIDHEADRRYLRAYELHARANNKLGRWGEAVSSMQKAVDIAPHLEEGLKLFEDDERKHKERQLMTVREVLALAGKGLDRDGRKKRYGQPFVMRYPADFDRVKKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCG9</accession>
    <name>A0A8H6CCG9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002520</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150275.1">
        <property type="nucleotide sequence ID" value="XM_037293446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCG9"/>
    <dbReference type="GeneID" id="59330933"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040023">
        <property type="entry name" value="WBP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173:SF10">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="5">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="48"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="218"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="308" mass="34293" checksum="ED50319A2F57677B" modified="2022-01-19" version="1">MSEYWKSTPKYWCKHCKTFVRDTKLEKTNHEATPKHQGNLKRFLRDLHKGHERDERDKQRAKDEVDRLNGVVSASGSQSGGAPWERRSAILPPSVAGNQQATPADRKRQLTQLAEMGVAVPEGFRREMAMAGDWQITSEKVVYDNDFLKKEEESEDVKLGGLNVGIRKRKFEGQEEEEEAGETVIRRGWGSTIRTYPGAGADEDDLATLLKSTKRVISEGEGLQPLGSGRSSRSQQADSHDATKEDQADLDSPAIKKEESAGPGKVLDTILTQTAVVDAPPESLKQEENSPDSGVVFKKRKAKPIRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDZ8</accession>
    <name>A0A8H6CDZ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10 13">Catalase</fullName>
            <ecNumber evidence="3 10 13">1.11.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_001700</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 13">Belongs to the catalase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.6" evidence="3 10 13"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151167.1">
        <property type="nucleotide sequence ID" value="XM_037292628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDZ8"/>
    <dbReference type="GeneID" id="59330114"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03132">
        <property type="entry name" value="GATase1_catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.180.10:FF:000003">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000050">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024708">
        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024712">
        <property type="entry name" value="Catalase_clade2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043156">
        <property type="entry name" value="Catalase_clade2_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041399">
        <property type="entry name" value="Catalase_large_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42821">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42821:SF1">
        <property type="entry name" value="CATALASE-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038927">
        <property type="entry name" value="Catalase_clade2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 10 13" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="8 10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="6 10 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 10 13" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="15">
        <location>
            <begin position="45"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038927-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038927-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6221732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="744" mass="82838" checksum="9FDD94C624069A8B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFL5</accession>
    <name>A0A8H6CFL5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein EFR3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000649</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151948.1">
        <property type="nucleotide sequence ID" value="XM_037291586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFL5"/>
    <dbReference type="GeneID" id="59329067"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039786">
        <property type="entry name" value="EFR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049150">
        <property type="entry name" value="EFR3_HEAT-like_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47766">
        <property type="entry name" value="PROTEIN EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47766:SF1">
        <property type="entry name" value="PROTEIN EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21072">
        <property type="entry name" value="EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="440"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="631"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="830"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="895"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="956"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1006"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="850"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="895"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="934"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="962"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1006"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1020"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1065"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1076"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1098"/>
            <end position="1111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1225" mass="134275" checksum="CDAA02C0093785C4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFT7</accession>
    <name>A0A8H6CFT7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Catechol 1,2-dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_000655</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151954.1">
        <property type="nucleotide sequence ID" value="XM_037291592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFT7"/>
    <dbReference type="GeneID" id="59329073"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018576">
        <property type="term" value="F:catechol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009712">
        <property type="term" value="P:catechol-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03461">
        <property type="entry name" value="1_2-HQD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039390">
        <property type="entry name" value="1_2-HQD/HQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007535">
        <property type="entry name" value="Catechol_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050770">
        <property type="entry name" value="Intradiol_RC_Dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711">
        <property type="entry name" value="DIOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711:SF7">
        <property type="entry name" value="INTRADIOL RING-CLEAVAGE DIOXYGENASES DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04444">
        <property type="entry name" value="Dioxygenase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catechol dioxygenase N-terminal" evidence="9">
        <location>
            <begin position="68"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Intradiol ring-cleavage dioxygenases" evidence="8">
        <location>
            <begin position="150"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6222608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="39879" checksum="158FB17301D5E752" modified="2022-01-19" version="1">MPETEEPLTLAPEEKNSQVNTNDNRIDHNQSHTNGITRGESATAPQTHRFDPSFTQSLINATGPKASPRMRKVMASLIRHVHDFARENEITVDEYMAGVEMINEAGRMSTDKRNEGQLLSGVIGLESLVDEITYKLATDATDAPTSSAILGPFWRANAPARKMGESIVHNITDGDDTFMHGKITDYLTGEPIEGAELDVWHTAPNGLYEQQDEDQVDFNLRGRFTTGIDGRYNFYCLRPTSYPIPYDGPAGKLLQLLDRHPMRPAHIHFIVKAPNYKPIITQLFDRRDKHITDDAVFAVKESLVVDFLPREGDPKARFELPYDFRLATYEEAKKNSLVGTTEESASFSANVVQGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFU7</accession>
    <name>A0A8H6CFU7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MED10</name>
        <name evidence="7" type="ORF">HO133_000670</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFU7"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019145">
        <property type="entry name" value="Mediator_Med10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09748">
        <property type="entry name" value="Med10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="146" mass="16282" checksum="D29E75E48AAF63EF" modified="2022-01-19" version="1">MAPMPENTVDDRLRDVIQNLFEIQSAVHGYLGPDTQQQLVRKIHQLTTSLSTLSTSSSSLPIGLPPEIIDYIDQGRNPDIYTRELVEAVQRSNQYLKGKSEAFAGFRDMLAEEIVKGIPECKEDVQRILEGNKAVVGEVTRQNGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGE7</accession>
    <name>A0A8H6CGE7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_000809</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152107.1">
        <property type="nucleotide sequence ID" value="XM_037291745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGE7"/>
    <dbReference type="GeneID" id="59329227"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22701">
        <property type="entry name" value="FHA_FKH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881:SF1">
        <property type="entry name" value="FORK HEAD PROTEIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="8">
        <location>
            <begin position="134"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="9">
        <location>
            <begin position="358"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="358"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="257"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="448"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="628"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="480"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6222761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="695" mass="75654" checksum="7C40CB75429B3199" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH01</accession>
    <name>A0A8H6CH01_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152519.1">
        <property type="nucleotide sequence ID" value="XM_037291084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH01"/>
    <dbReference type="SMR" id="A0A8H6CH01"/>
    <dbReference type="GeneID" id="59328563"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="226" mass="25887" checksum="E00351A46E799304" modified="2022-01-19" version="1">MNSYAARLASFTSWPHVYPSASDVARAGFHREFDPTFPSPDLTVCSLYDLDPCDWDSDNPFDFHATRSPKCPFVRATQQKKKDRNQKTRRQQYKRRSLQATQPAHQSTQLATQQEHSVEQEEELQQANAEKTAEKTMENTAEKPSDNKATIVKNTAHLSTIPRQIATQQAENDTTIWPIIPTALIWYITAMSTQEKDLLRRSQPNHYGHGNWAPAGGYRTMHIWTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHE3</accession>
    <name>A0A8H6CHE3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152486.1">
        <property type="nucleotide sequence ID" value="XM_037291051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHE3"/>
    <dbReference type="GeneID" id="59328530"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="297" mass="33090" checksum="9E7E92E7403CA7FA" modified="2022-01-19" version="1">MAYSSSFQPLASTNPQEFTTETSVTSLISGLQSQYDTSSSTLSTSSTHAVTSGSRAVLSSVGELPKSCGFLTLAGELRNIIYGYLITSGQIEILRVSRQLHDEANGLLYKQGICRLRFELASIKHQPKSLSQIRIPSNNKIQNFNLLISPLTTWRPLANRGFVHSVQGSGDCHIILVWEDSWSFALDRSIAHFNFIEFLSTFKLLTLRIHFSHTLHPMDRRNRTVVERRHTQILQSVAASLPSALGSPEWKTETCPSACYQTSTCARAQPSMNPFPNAQYLEFHPRKGREIAGGVHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHG2</accession>
    <name>A0A8H6CHG2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152516.1">
        <property type="nucleotide sequence ID" value="XM_037291081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHG2"/>
    <dbReference type="GeneID" id="59328560"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="328" mass="36404" checksum="9CA56A65ED849C49" modified="2022-01-19" version="1">MDRFIATSTAPRPDWPLPLLPSHSLINVPKERKRHCRLLPADMVYLSLKQMADAADHEEQKKLRNQRKKLDFKEVEFMEQEFQKWADECQKLQKKKKSKMHKQTQKKIAVVEPNSFDEMIDSVDTLLSSLTPPTTETSSPTKTSNTAATTHPSPPTASTIQIAELDACETAITKPSLPTDTPRAKVHVPTTTAIHTPPPPPITFTTALHSNPPTRTDLAFARNLSTALHSDQDQRTIATKPQPNAPTLIAVTHHGRKTNVFKAAIGAMTRCRGGKKRAVIEAAVDVVARQRGKGAAEKKKGRKERMGLSFSEAVDKGFRGVFGEERGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHK5</accession>
    <name>A0A8H6CHK5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TM7S3/TM198-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000465</name>
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    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="entry name" value="DUF4203 DOMAIN-CONTAINING PROTEIN"/>
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            <begin position="1"/>
            <end position="24"/>
        </location>
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    <feature type="chain" id="PRO_5034901620" description="TM7S3/TM198-like domain-containing protein" evidence="7">
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            <begin position="25"/>
            <end position="1114"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="117"/>
            <end position="138"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="145"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="178"/>
            <end position="197"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="204"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="229"/>
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        </location>
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            <begin position="255"/>
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            <begin position="127"/>
            <end position="329"/>
        </location>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1114" mass="121522" checksum="B3FE4C7338AB6C34" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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                <person name="Walston R.F."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <evidence type="ECO:0000313" key="4">
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8H6CIB9_9LECA</name>
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        </submittedName>
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    <reference evidence="2 3" key="1">
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                <person name="Walston R.F."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="171" mass="19352" checksum="0B147F9FA305E279" modified="2022-01-19" version="1">MDDAIKEKLRLATLHNGELLRIGKTQGQEREHIARTQAQAREHRKLTHDAIHDGAIARQKALNEENYEHRERLLKLERQYASHETGVEPRESVPESENVPEDFERSGGGGTEDNESVEAFVLPQDDLRVECATDGKVSRDFGSKTAAMFETASSVGLFWTIPLRGERKYDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIC7</accession>
    <name>A0A8H6CIC7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FMN hydroxy acid dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010581</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153067.1">
        <property type="nucleotide sequence ID" value="XM_037301433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIC7"/>
    <dbReference type="GeneID" id="59338971"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000132">
        <property type="entry name" value="FMN dependent dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF75">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE (AFU_ORTHOLOGUE AFUA_4G07050)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 7" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="8">
        <location>
            <begin position="32"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="336"/>
            <end position="340"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="359"/>
            <end position="360"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="421" mass="46574" checksum="698E660CC7CA4001" modified="2022-01-19" version="1">MSDPKNPNIDKRSTPQYALYQRENFWKTNTGEVPPFNTEPGKLEELAKAKLTEGGWYYASSNAGQSYTHLANRQAFFRHRIIPRQLVDTNLRDTTTTIFGHKVSAPIGFAPIGINVIYNPLGELPVAKVAKELNLPYALSTAGSNPIEKVAEANGQGPRFFQLYMPHDDELTVSLLTRAFNSGFDVCILTTDTWQLGWRHDDVATGNYAFYHGIGADLGLSDPVFQKRLKEAGIDPVKQPNEAGAMWIDNVWHGRAWSWEKIPWLIETWKKISGGRPFCLKGIQSVEDAKKAVEVGCDGIVVSNHAGRQVDGAIASLDALENIVAAVGDKTYIMYDSGIRGGSDVLKALALGAKFVWVGRLWVWGLSIMGEAGVRHVMKSLLADFDILMNVGGFQNIEQIDRTALESYPKAYAMIAERSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIR0</accession>
    <name>A0A8H6CIR0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153288.1">
        <property type="nucleotide sequence ID" value="XM_037301654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIR0"/>
    <dbReference type="GeneID" id="59339193"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF41">
        <property type="entry name" value="CLASS II ALDOLASE_ADDUCIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G01330)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="2">
        <location>
            <begin position="58"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="295" mass="31979" checksum="090A8F94F070A1CD" modified="2022-01-19" version="1">MAPPSATTTQSETNGSYPSTKAPLEPGLSESRARGQMAHFPGPPTFTDKYEEREYLKGRLAAAFRIFGKYGFDEGVAGHITLRDPVDPTTFWVNPWGVAFSLIKRSELIQVDHSGKIIGGGSVKLLNTAAFMIHGAVHKARPDVMCAAHSHSIYGRSFCTLGQKLDTITQDSCAFHDDHVLYSTFNGIVLAEKEGEEIAHALGDKKAALLQNHGLLTVGGTIEAAVFWFMSLEKCCHAQLMADAAAGGRGHETVKINDEDAAFTYKTIGTPRGGWFSAKPLFDVIEKECGADYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ04</accession>
    <name>A0A8H6CJ04_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153305.1">
        <property type="nucleotide sequence ID" value="XM_037301671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ04"/>
    <dbReference type="GeneID" id="59339210"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="128" mass="13659" checksum="792FE36BAEA95A35" modified="2022-01-19" version="1">MDLLSPSDEPPAANVPIEKAPPSKPAARFDDTPSPMRADGNSPNFKPLISPEKDIRPGLTDLTREVPRGPITAPRHVRSGTTVPGQTASRDTVNPTQDVRSGAGASPKAGVHVKPTNLKNRQRKTLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJV8</accession>
    <name>A0A8H6CJV8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010061</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153734.1">
        <property type="nucleotide sequence ID" value="XM_037300917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJV8"/>
    <dbReference type="GeneID" id="59338453"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="RanBD1" evidence="8">
        <location>
            <begin position="846"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="308"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="810"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="336"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="378"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="456"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="543"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="566"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="826"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="838"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="986" mass="105894" checksum="C2981E6DA93E1B34" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLM3</accession>
    <name>A0A8H6CLM3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154228.1">
        <property type="nucleotide sequence ID" value="XM_037300380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLM3"/>
    <dbReference type="GeneID" id="59337914"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="425" mass="46799" checksum="18CAD858D7C0A27E" modified="2022-01-19" version="1">MASSFSSLTTKTSILSPAILAQSPSLPAVVKLLNYSYNQSLISGHNSRLLPPDPTTRLQSLTQLSSELEPDGFTILMFSSQISQDEDIEGLMTNRANGMTLIATVSAKPYVPTKPEGMGQRAHLLFKRPPPDASKPETNNERGKSGDDDEERWPKWEILANAVHPSLQVVVDPSESYHAAHNESLQTALGETLDPVPESLNSRTGSTTASTAEHNFADGDEDSIQGSQPDMFSTVSHQHLRRLSPSTSSTTTPKLITNLTGSEADRCRERVIDEVSSWDGCSLNKESIRKRLIFKVDGNGIPHNRPPRGDIDEERKRLEYYSVTRKTEVTGEIKSRASSSTSPTPPAQHHRNSVNGDGDPETENTREEAVGKGKVMLMLSTMLEINESYYLKRGFVTTAVRRFEPGTMGSRDGFSVAEMVRWVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLS6</accession>
    <name>A0A8H6CLS6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Stress response protein NST1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009790</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">May act as a negative regulator of salt tolerance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the NST1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154497.1">
        <property type="nucleotide sequence ID" value="XM_037300649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLS6"/>
    <dbReference type="GeneID" id="59338185"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051195">
        <property type="entry name" value="Fungal_stress_NST1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025279">
        <property type="entry name" value="NST1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31780:SF10">
        <property type="entry name" value="LD36051P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31780">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NST1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13945">
        <property type="entry name" value="NST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="143"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="349"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="460"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="558"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="958"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="382"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="558"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="591"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="784"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="797"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="832"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="961"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="983"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1005"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1068"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1087"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1121"/>
            <end position="1137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU049441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6225788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1265" mass="138944" checksum="4F0B51D9985EA0ED" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CME9</accession>
    <name>A0A8H6CME9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008968</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154656.1">
        <property type="nucleotide sequence ID" value="XM_037299830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CME9"/>
    <dbReference type="GeneID" id="59337363"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF16">
        <property type="entry name" value="ANKYRIN-1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="248"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="43570" checksum="9868F00E91907F78" modified="2022-01-19" version="1">MIQIDYLFSPWMLMAITSTTIVVPSLHSWNITLRVCRVVPDSAIIFRFALDGNLQGMKMLFDAGSASAHDVDASTGTSPMDVACGRILGGTASPEITTALTEDFDIEEHLEERQLPVLHKIVLGLAEADLETQLRLSTMVKIDALDYQGRTALSWAAIRGDSASLNLLLKYGADPNVVAYNGDAPLRYATRAPDPRCVRPLLESGADVNAVNAWKGNPLAYTTAFRDDLRYLKPLLEKSVDMEHKDRHGRSALMRAVLNNRPNLVKCLLGNGAKLAEADAWGFRPLMVAVERKFHAVAHIILQSGTSELGVEPLSSVLEIALVSGDRQTVEMLEGLQSRVLNAGEEDSEVVGAATNDLKDLGTQNDTTQLQQAARALTTGFQASKPAVPVLVFRKEKVKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMH8</accession>
    <name>A0A8H6CMH8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154688.1">
        <property type="nucleotide sequence ID" value="XM_037299862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMH8"/>
    <dbReference type="GeneID" id="59337396"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14096">
        <property type="entry name" value="STKc_RCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="139"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="507"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="535"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="571"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="586"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="638"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="651" mass="72254" checksum="28F67E8144CE8DD7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMM8</accession>
    <name>A0A8H6CMM8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009089</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154776.1">
        <property type="nucleotide sequence ID" value="XM_037299950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMM8"/>
    <dbReference type="GeneID" id="59337484"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01370">
        <property type="entry name" value="KISc_KIP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000053">
        <property type="entry name" value="Kinesin family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF13">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF19 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="9"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="645"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="771"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="799"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="882"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="983"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="704"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="737"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="812"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="902"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="986"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="135"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6226223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1010" mass="110013" checksum="B981B323529BEF1B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMR3</accession>
    <name>A0A8H6CMR3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008956</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154644.1">
        <property type="nucleotide sequence ID" value="XM_037299818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMR3"/>
    <dbReference type="GeneID" id="59337351"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12324">
        <property type="entry name" value="RRM_RBM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008111">
        <property type="entry name" value="RNA-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033744">
        <property type="entry name" value="RRM_RBM8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45894">
        <property type="entry name" value="RNA-BINDING PROTEIN 8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01738">
        <property type="entry name" value="RNABINDINGM8"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="39"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="117"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6226091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="154" mass="16485" checksum="045F2F8CF99AFE45" modified="2022-01-19" version="1">MASNDMEIDSSPPRNGSKPSTTDQHTSSGAVAVRSIEGWIILVTNVHEEASEEDLQELFGEYGDIKNLHMNLDRRTGYVKGYVLVEYPTVANAKAAIEGANGTKLLDQTIGVDFAFVRPPPKEGKQGQGRGGGKGGRGARNKSRSPEGDGVKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMZ3</accession>
    <name>A0A8H6CMZ3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154762.1">
        <property type="nucleotide sequence ID" value="XM_037299936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMZ3"/>
    <dbReference type="GeneID" id="59337470"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="195" mass="21986" checksum="699664F5C6FDA832" modified="2022-01-19" version="1">MATENDTLPLLPPPANDDPPSFDSHPEFYAPAARQRTRLQSAPNLPPTDSSPRGSPTNPFRPPQNNLNQQSQDTDAYTPAKYRGHATEEEYLAALRAWAEEKLYTQPADKSTLVGFYGAETMKERIARQPPPLKMRIGRKRKESRADESAVRERQGAQEDEMVDGEIARERGNGEGEGRRRRSSLGNWLSRKRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN97</accession>
    <name>A0A8H6CN97_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009391</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155078.1">
        <property type="nucleotide sequence ID" value="XM_037300252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN97"/>
    <dbReference type="GeneID" id="59337786"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010856">
        <property type="entry name" value="Gig2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30613:SF1">
        <property type="entry name" value="DUF1479 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G09280)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30613">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBIU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07350">
        <property type="entry name" value="Gig2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="462"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="880"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="911"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="923"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="933" mass="103989" checksum="6D89F7D9AEEAFB93" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNL6</accession>
    <name>A0A8H6CNL6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008421</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155288.1">
        <property type="nucleotide sequence ID" value="XM_037299287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNL6"/>
    <dbReference type="GeneID" id="59336817"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF84">
        <property type="entry name" value="VACUOLAR MEMBRANE AMINO ACID UPTAKE TRANSPORTER FNX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="77"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="539" mass="57685" checksum="428782935B6DD489" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNT2</accession>
    <name>A0A8H6CNT2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tetrahydrofolate synthase</fullName>
            <ecNumber evidence="3">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_008294</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155162.1">
        <property type="nucleotide sequence ID" value="XM_037299161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNT2"/>
    <dbReference type="GeneID" id="59336691"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11061">
        <property type="entry name" value="CYP67-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF5">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase central" evidence="14">
        <location>
            <begin position="474"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6226853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="903" mass="100990" checksum="94610A617FA152CE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQR5</accession>
    <name>A0A8H6CQR5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007618</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155824.1">
        <property type="nucleotide sequence ID" value="XM_037298488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQR5"/>
    <dbReference type="GeneID" id="59336015"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07216">
        <property type="entry name" value="Pat17_PNPLA8_PNPLA9_like3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="6">
        <location>
            <begin position="13"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1123"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="4">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="62"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="198"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1146" mass="129097" checksum="D2AB0B21055E35B2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR04</accession>
    <name>A0A8H6CR04_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155897.1">
        <property type="nucleotide sequence ID" value="XM_037298561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR04"/>
    <dbReference type="GeneID" id="59336088"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="442" mass="50398" checksum="9F1BA8A28275D278" modified="2022-01-19" version="1">MPSSAAHNLLSLRNSSLASAPLNITNPALKAPEEITNLTYSPWPEQPFGITLLGPGLLFLNVYLLIGAGRKFQSSPMVSVTELRHFLQDFVDNIKREHPVPGCVPRHATQSTIDISSYTKWLIAINEGPFRGRLPTAVALVAFDALGKLLGRYGPSEISWGIKEVGTRTPWAYGSLTIESIEGFTVNKSSSIENGGFRTAHPYWYRKLAKLPRDAQKPSFRLLETCLLTEDNKKSSTDEEIRIILARWKGFSLLRGLYVDDKVSEEFDRFRAAFASGLTREKGVTIVPPSLDDASIKTSHVNRVQFIQNSRFVGDFKAMFHRIKTGHIENRSWAGGEPNEGMYARRHNMFKRGAHNELGFDWTFDFERSAMVKALCTTSQDRLMNAEDTYWWDHVRLRLGSPFVEDAIHEYWDEIHEMRKERVKEERRTAERTKMKGDRMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR15</accession>
    <name>A0A8H6CR15_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_007632</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSE2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155838.1">
        <property type="nucleotide sequence ID" value="XM_037298502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR15"/>
    <dbReference type="GeneID" id="59336029"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16651">
        <property type="entry name" value="SPL-RING_NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026846">
        <property type="entry name" value="Nse2(Mms21)"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330:SF1">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11789">
        <property type="entry name" value="zf-Nse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="12">
        <location>
            <begin position="278"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="151"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="373"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="457"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6227904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="469" mass="51317" checksum="8DC9FB5F5613B33D" modified="2022-01-19" version="1">MASSARSRPSATSNRQRSGPTPLPTYQPPQHPLNENAIRAVQDLPRNHKLDSLKARLNAANNHLAGAAVDINDRLVAQIALHEKSRARREKQGSQEDNEEEEMAMDEKRQQTIELTGRLESGVRKIIDAKFEVEGVEKALRELETNVANNRGAIAPTQSTLGASQFRPNRRRRGGDADAEDSEDDTESAGENGGALDFIKRKVAEQNSTYQNETMSFRYAQNNDYVGFKKLVYDAQFPGDDAPLMPHSSTWFSDQSPNMSDRNTRGAAAAPGSQTAGNDDDLAVASERISIKCPITLLPMKDPVTSTKCPHSFEREAILSMIDASDVRVDGSGRRGAGGKKAMQCPVCTVMLTADDVAANPVLVRKIQRIQAAENAQIQDDSDDDDVRAPTSGRLRPEEIGSSPARTRTTQVKNERRSQALRNKSREVSMVPNSQVQGLVNEGQGRPSSSAATQVVDLEEDDEDEDAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR23</accession>
    <name>A0A8H6CR23_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007741</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155947.1">
        <property type="nucleotide sequence ID" value="XM_037298611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR23"/>
    <dbReference type="GeneID" id="59336138"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF434">
        <property type="entry name" value="GB|AAD18119.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="24"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="228" mass="25353" checksum="348DF5FF5D9A9489" modified="2022-01-19" version="1">MAEEFLAQLRYVRRGVLEKGDQRCFICMEDYGTTPSENGTIERAVILPCNHIMGSECISTWLSPRAQGGAGNNTCPVCRHVLFRRVQRPPIPQPNDAEHMRIHTVLVNRCVGLSAQLGLNHMPEIAGMARWIANHIHDIFAFEMIERENDDRFVRSVAAASIYMASHLLGDGRSLEMIESCALSGDDPIRSAYALLYLHRYDIINTQLLARVGMGLGSIGDVLPPALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRE6</accession>
    <name>A0A8H6CRE6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156126.1">
        <property type="nucleotide sequence ID" value="XM_037298790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRE6"/>
    <dbReference type="GeneID" id="59336319"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="394" mass="43138" checksum="A7FF945BF3E76A01" modified="2022-01-19" version="1">MPPKNSFTSSFSASDVNNEVEKRYRSMQEHIRRAHPEHYIAKLPATEESFQLMINTPPSERPQPPPPPPPPPPPPPQPFGNSVYGHDRDAYIMRSSPAPPRTLEDQYPAAATAAVALAQLHSHQPHSEWGSEADYASENEASQPPMRPSIELPPIQTHLNQQLIHPFHSPRPRELLPSMMAHSPPGRSSTLPPLQRKDSIQRKEKANRPRKSSITQNSRKPKHEKTKSKDYSRRLSIEGRKAFSAEPPGAAAAAAMGKRWEDLIDAATSATEADSDRDLTPIPQSPSSVKRASMPPFSSVSHNNQSYKASPLHRALTPPSSHSFTGPRLFPSVETSLDSSTSTQSSGRNFHIPSAGLSNSSDTTSPTFYSQPVQIYSYGQTKALSEVPGAGATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS21</accession>
    <name>A0A8H6CS21_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008253</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156457.1">
        <property type="nucleotide sequence ID" value="XM_037299121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS21"/>
    <dbReference type="GeneID" id="59336650"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000007">
        <property type="entry name" value="Mitochondrial enolase superfamily member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018110">
        <property type="entry name" value="Mandel_Rmase/mucon_lact_enz_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046945">
        <property type="entry name" value="RHMD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794">
        <property type="entry name" value="ENOLASE SUPERFAMILY, MANDELATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794:SF58">
        <property type="entry name" value="MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00909">
        <property type="entry name" value="MR_MLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="6">
        <location>
            <begin position="765"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="294"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="294"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1045" mass="118287" checksum="4834A519C4BB885B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSE5</accession>
    <name>A0A8H6CSE5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein TDA6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156410.1">
        <property type="nucleotide sequence ID" value="XM_037299074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSE5"/>
    <dbReference type="GeneID" id="59336603"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009291">
        <property type="entry name" value="Vps62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48172:SF2">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING PROTEIN 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06101">
        <property type="entry name" value="Vps62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="456"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="55249" checksum="22F17B127E3E4DC6" modified="2022-01-19" version="1">MGRSPRSLDGGLGDGENVSREAVLMILEKEGDGVEKAPQNYSSPCRRSHDVAMKNRRTSVKLPILLGFILVAFIFTTPHTVPVIPTRLRGQVGAWLKMCGSHLGWQDPSVIDIRTRPEDLAKDPRVMHEIPQYVLDYAPLVHLYSEEQFWPCDIKEHLFHVTPELNYTPIQGLPQLNLSNLDQLNEFEQGRHVFLTSNDNVEDRPDWLGGQINIPDDFSDDSKTDCSDSRATSARFRKQSHGGHSDAPAVLLTINKGDGIVDAFWFFFYSYNLGNIVFNIRFGNHIGDWEHTLIRFEHGKPKVVFVSEHFFGSAYSYSAVEKIGNRPVVYSAVGTHAMYATPGTHPYILPLGILHDETDRGPLWDPALNSLTYTYNHVTDTLHPSNRTPDAPTQWFYFSGKWGDKFYPLNDNRQYGFAGEYHYVNGPLGPRFKHLGRRKVCQGRYSDPCVIKDQLDPDEVREWPGLGEGDDWPDPEMRPPGLPEGGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CTH0</accession>
    <name>A0A8H6CTH0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006947</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156478.1">
        <property type="nucleotide sequence ID" value="XM_037297843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CTH0"/>
    <dbReference type="GeneID" id="59335347"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF15">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="204" mass="23321" checksum="1DD19035455B3590" modified="2022-01-19" version="1">MEDDWLPGKTVLASIAIEHLVNTFRSQTQNEQTLLNLLTSLLKQLLLHKSAVPEGIRSLYEQHNNGSKTAQPILDEVFESLQLIAASFSQECIVVDALDEVQQHGKLLQSLLPKVFELQATKPFNLMLTSREIPRLTEEVHSHFTLKVRASDDDVRRHVDGRMDELPRFVSRDPKMQQTIKDAIASAVDGKYVPSRIWSHWFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6L7</accession>
    <name>A0A8H6F6L7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha/beta-hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006875</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146972.1">
        <property type="nucleotide sequence ID" value="XM_037297771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6L7"/>
    <dbReference type="GeneID" id="59335275"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049492">
        <property type="entry name" value="BD-FAE-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF3">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20434">
        <property type="entry name" value="BD-FAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BD-FAE-like" evidence="3">
        <location>
            <begin position="16"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="260"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="36671" checksum="75EE481744755778" modified="2022-01-19" version="1">METITYSRVGSLDIKLDLYIPPEPKQGLVPALVHFHGGGMIAGCRKTLFFQHWLKDAMLEHGILFISADHRLLYPSTAFDIIADVKSLFAFLASPSFSETHLPSGVTLDASRIAVSGESGGGYATRAAGIVAEPKPKALLLQYAQGGQLLDDHWLAVKDRDVPDPEGGIVTKESVAHLLNDTQEPVSDDPMPFLGDDAPNGDSGRTKLLLWWWRTGGFVDVVLGVNGMSKFLRGLTLAQREGFLPEALRKVILQTQLTEKFPPTFLLHGKDDKLVLPAESQLTYNRLTELGVEVELHMLEGAGHTLIDEKNPPNLAAGAKDAQQKAVEFLVHKLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7R6</accession>
    <name>A0A8H6F7R6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TBP-associated factor 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_006260</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147283.1">
        <property type="nucleotide sequence ID" value="XM_037297158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7R6"/>
    <dbReference type="GeneID" id="59334661"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007900">
        <property type="entry name" value="TAF4_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05236">
        <property type="entry name" value="TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID component TAF4 C-terminal" evidence="10">
        <location>
            <begin position="446"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="72"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="556"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="632"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="145"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="191"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="238"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="496"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6217848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="728" mass="78223" checksum="3682767D874F7A83" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7U2</accession>
    <name>A0A8H6F7U2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147299.1">
        <property type="nucleotide sequence ID" value="XM_037297174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7U2"/>
    <dbReference type="GeneID" id="59334677"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="194" mass="20297" checksum="B5A389602E155783" modified="2022-01-19" version="1">MSASRNADAVSNQPGQFGSHIERDEPMREPKHKPGVLVGNDAAPEFSAKTLPPGSAPADRTFKPNPISEVPGQADNSSVERDHGKESTKTTASSTLGGATSGDVHTGMGHPGQGQTSNELRHDGQHTNKKHGGGLAGVGASVGSSNQMVDKDTQPKERAIEKEEGKFAGTRGDKGEEYPQPPAQNREPESAESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7X1</accession>
    <name>A0A8H6F7X1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005907</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147992.1">
        <property type="nucleotide sequence ID" value="XM_037296811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7X1"/>
    <dbReference type="GeneID" id="59334312"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055795">
        <property type="entry name" value="DUF7371"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24086">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034519991" description="DUF7371 domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7371" evidence="3">
        <location>
            <begin position="353"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="547" mass="56422" checksum="3C73D9D903E5478C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7X4</accession>
    <name>A0A8H6F7X4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147463.1">
        <property type="nucleotide sequence ID" value="XM_037297338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7X4"/>
    <dbReference type="GeneID" id="59334841"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="70" mass="7751" checksum="0A30FEB0F0B9EC71" modified="2022-01-19" version="1">MTPKIFEMDNDMPDWTDDFDFGAFLIIPPDDVDQGDIGPGMHGDQTGQVANTYFYPATHLDSLSDDEPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F889</accession>
    <name>A0A8H6F889_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147734.1">
        <property type="nucleotide sequence ID" value="XM_037296553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F889"/>
    <dbReference type="GeneID" id="59334050"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="341" mass="37638" checksum="528563B3FC31B1A6" modified="2022-01-19" version="1">MSTSMAARVRRLKNSLQFWADDPTGYYIGEHLGGSSSNDQEDSVTLAFIQVAAKIQLFCFKSQEMKCYVAMAFLWVLQLLVSHSHASAISGRLLNGAVFLGILNSARRRSDYSIKTPKYTLPRILQGTPRGEGFNAEAWSTAPHAATGFLQTYLWSDKHWSPGNPSPTSTVSAHVTSQASAVRAVWTHDLQTINNETSKLSLPQLREYETWIEGYEHSGPYEDIILNQPLFAMTNISSLTTIWIAPKSNMNSVTTGLVVIGPIEEKTGQRYGLGCSIDARWNKAQHIMTQSSSYSLENTGKYIGATISSARSSADIVDKALTIIDSDGNWRHISNQDGLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F896</accession>
    <name>A0A8H6F896_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148367.1">
        <property type="nucleotide sequence ID" value="XM_037296385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F896"/>
    <dbReference type="GeneID" id="59333881"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031833">
        <property type="entry name" value="DUF4748"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41800">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41800:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15932">
        <property type="entry name" value="DUF4748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="110" mass="12343" checksum="347D8DC105924E3E" modified="2022-01-19" version="1">MDWLGCLDPGGGAYVFAKRSVNADKKARHEEEMKRRRLKDALDAEFYAKPPSPKQGHKRPHDHANSPSTEASHDPAPTEHPLENGGTKDQEEARYVASKPYKSKKGDRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8V9</accession>
    <name>A0A8H6F8V9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein SQS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005131</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SQS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148741.1">
        <property type="nucleotide sequence ID" value="XM_037296043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8V9"/>
    <dbReference type="GeneID" id="59333537"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02646">
        <property type="entry name" value="R3H_G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034082">
        <property type="entry name" value="R3H_G-patch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051189">
        <property type="entry name" value="Splicing_assoc_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14195">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="11">
        <location>
            <begin position="665"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="G-patch" evidence="10">
        <location>
            <begin position="793"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="190"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="272"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="481"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="590"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="275"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="481"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="598"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="837" mass="91773" checksum="3AF8F1DBAD976CBB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F961</accession>
    <name>A0A8H6F961_9LECA</name>
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        <recommendedName>
            <fullName evidence="6">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="6">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003625</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149235.1">
        <property type="nucleotide sequence ID" value="XM_037294547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F961"/>
    <dbReference type="GeneID" id="59332036"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF687">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="402"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="77"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="531"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="805"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="230"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="536"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="818"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="875" mass="95567" checksum="40C4C5C203E17F9E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9G0</accession>
    <name>A0A8H6F9G0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004087</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149053.1">
        <property type="nucleotide sequence ID" value="XM_037295006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9G0"/>
    <dbReference type="GeneID" id="59332496"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="42"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="252" mass="27765" checksum="43D7C93F76C2F0F5" modified="2022-01-19" version="1">MPSLLSSTEIEYELAHIHDNRAPNVVISAAICISLAITAVLLRLLARRLSKARILADDYMILFALIICMGQVAACLYSVHLGAGKHVILLKDPAAYAKTIIAMEVLYCIGTAAFKYSALLLFHRIFGSVPRFTAFLWCFAFVILANNIAEVFLSIFQCTPVHKAWDLNVEGSCVNILLAACIPGSLNVISDVVTFLLPIPLIWNLHMERNRKIQLVGIFLLSGLYVPHQRSLSLQSSQRHHADPANLTKASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9V9</accession>
    <name>A0A8H6F9V9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein transport protein BOS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_002983</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the BOS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149985.1">
        <property type="nucleotide sequence ID" value="XM_037293907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9V9"/>
    <dbReference type="GeneID" id="59331395"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15863">
        <property type="entry name" value="SNARE_GS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000054">
        <property type="entry name" value="Protein transport protein BOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF1">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="34"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6220550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="189" mass="21778" checksum="1CFF124A14CFA583" modified="2022-01-19" version="1">MAKKELIPSKQEKAHERLKNFRIELADYRQSFDRLRKDRDDSQTAQNRTELLGRRPHHTATPENPYSQSSLAQNSTFAPPKPQSLSFGAAPQDYTRETHALREQTFMSSTNAALDEYLERGRAVLGDLGDQREMLKGTQKKLYSVANTLGISGDTIRMVERRSRQDRWIFGAGAIIFFAFCWLVIHYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAB7</accession>
    <name>A0A8H6FAB7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002981</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149983.1">
        <property type="nucleotide sequence ID" value="XM_037293905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAB7"/>
    <dbReference type="GeneID" id="59331393"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032156">
        <property type="term" value="C:septin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000196">
        <property type="entry name" value="Cell division control 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="5">
        <location>
            <begin position="93"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="440"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="472" mass="53688" checksum="4874EF1BBAEF6C0A" modified="2022-01-19" version="1">MATNGPLSQAPRQSPAPQSGPPVPNGKPTSPYNERTNPMSPTQQIASQTVSSKDPKAAAQAASDMRNVVRRKLTGYVGFANLPNQWHRKSVRKGFNFNVMVVGESGLGKSTLVNTLFNTSLYPPKERKGPSLDIIPKTVSIQSISADIEENGVRLRLTVVDTPGFGDFVNNDDSWRPIVENIEQRFDAYLDAENKVNRMNIVDNRVHACVYFIQPTGHSLKPLDIEVMRRLHTKVNLIPVIAKSDTLTDEEIMSFKARILTDIHHHSIQIFEGPRYELDDDETIAENQEIMSKVPFAVVGANSEVTSPEGRKVRGRRYPWGVIEVDNEDHCDFVKLRQMLIRTHMEELKEHTNNALYENYRSDRLTQMGVAQDPSVFKEVNPAVKQEEERTLHEQKLAKMESEMKMVFQQKVAEKESKLKQSEEELYARHREMKDQLERQRAELDEKKSRVESGRPLDEKTKKKGFSLGGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAD2</accession>
    <name>A0A8H6FAD2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">O-methyltransferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002052</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150632.1">
        <property type="nucleotide sequence ID" value="XM_037292979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAD2"/>
    <dbReference type="GeneID" id="59330466"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF1">
        <property type="entry name" value="HYPOTHETICAL O-METHYLTRANSFERASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="137" mass="15205" checksum="7E7B2F2BECFCB209" modified="2022-01-19" version="1">MTRCLFVDVGGGRGKSSRTFRNDHPELNGRVVIEDLPQVAEGQEGVEVIARDFFAPQPVKAPGANIYFFRFVFHNWPDSACRETLLHTIRAMTPTSPHCRQDSSSATSSSQTPTRPLSKPLTDLQVIAFGGMERTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAN6</accession>
    <name>A0A8H6FAN6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150254.1">
        <property type="nucleotide sequence ID" value="XM_037293425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAN6"/>
    <dbReference type="GeneID" id="59330912"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="208" mass="23407" checksum="15035E0EF021070F" modified="2022-01-19" version="1">MSTNSEFVLHETSSLYRRKDHQARANRIAEQKLLAAWPEPAQNSTSKNQWPNCQSDKHESEWRQQCSKRVIKNAQSGAESYDKSHDEPEKTPLPAPPIAQNTILDGFQQAEARKDTVNDDCFVRRDTTKELLELMVLGTPPVDNVRGRDAEAKKRQTKIDDHGPLAQRERASGEAQGASDTGDDAQTEREHDGQDHEGNGDLPEERIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAR1</accession>
    <name>A0A8H6FAR1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein transport protein SFT2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_002649</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Nonessential protein required for the fusion of transport vesicles derived from the endocytic pathway with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150403.1">
        <property type="nucleotide sequence ID" value="XM_037293574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAR1"/>
    <dbReference type="GeneID" id="59331061"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="216" mass="23823" checksum="223252DAADA9CEFB" modified="2022-01-19" version="1">MASTSFRDSMNSMGWSRRDPDIPVTTSSSTPILSKLQSINPFSNAGYVRLPTQNEGPGAPLPAPNRREEEEGFFALSRWDRLLVFGACNVAALACFVLCFALFPILWPVPRKFAILWSFGSVLFLASWAVLMGPATYAKHLISGPRLPFTAAYFGSIGLTLFFAIKLHNTVLTLLSIIVQLVSLVWYLVSYFPMGGTGLRFAARFGTNRVAAWMTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBL5</accession>
    <name>A0A8H6FBL5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001807</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM50/omp85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151274.1">
        <property type="nucleotide sequence ID" value="XM_037292735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBL5"/>
    <dbReference type="GeneID" id="59330221"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.50:FF:000008">
        <property type="entry name" value="Mitochondrial outer membrane beta-barrel protein Tob55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF18">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="7">
        <location>
            <begin position="172"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="514" mass="55838" checksum="E933A8B734F2290F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC58</accession>
    <name>A0A8H6FC58_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152373.1">
        <property type="nucleotide sequence ID" value="XM_037292011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC58"/>
    <dbReference type="GeneID" id="59329497"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="658" mass="72562" checksum="F4E6A89F70F1F31C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC77</accession>
    <name>A0A8H6FC77_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000819</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152117.1">
        <property type="nucleotide sequence ID" value="XM_037291755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC77"/>
    <dbReference type="GeneID" id="59329237"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF112">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_1G02370)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="4">
        <location>
            <begin position="353"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="90"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="476" mass="50445" checksum="251FA10449DB0C4C" modified="2022-01-19" version="1">MTGAGGSIGRACVLQFAHHGRTRIAGLAATASALSSLPAATAVEFLPSTVDQASDADVDRADRDTVARFGRIDYAVNNAAIAAPFRPTGASLTQDSDRVAGRSTCADPLPAASASASSSDGPGGGGGGGNGDHRGRSRGSILEVCSSLGLVAMPADGLYIISKHGVLGLVKTDALDYGKRGIRVSAVCPGFVDTTLLTPEYRVLLDWSIRKNPGILFLITADMPILPHDIHLGQVDPKTYPDLGVASVEKKRIEANLITGSIFDGLVTEKTMTVAQLLPPISADETPIIRCLGLKYRDHAREAKMPIPSHPVLFIKPRAALCGPSPRKISVPKHCQDGTSDYEAELTVVIGKTGRDIPQKKAVGWSFSKGFDSSAPIGPVLVAQEQISDPHDLRIKAIHNRNVVQDSSTEEMIFGVSEMIAFLSQRTTLERGSLIMTGTPPGIGAMRDPKVVLNHGDDMRVEIEGIGMLINEIYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCE9</accession>
    <name>A0A8H6FCE9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000047</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152422.1">
        <property type="nucleotide sequence ID" value="XM_037290987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCE9"/>
    <dbReference type="GeneID" id="59328466"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF27">
        <property type="entry name" value="ACL091CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="3">
        <location>
            <begin position="211"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="667" mass="73748" checksum="A06CDA12EAD201CE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCF3</accession>
    <name>A0A8H6FCF3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein transport protein SEC22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_000062</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152437.1">
        <property type="nucleotide sequence ID" value="XM_037291002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCF3"/>
    <dbReference type="GeneID" id="59328481"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15866">
        <property type="entry name" value="R-SNARE_SEC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000007">
        <property type="entry name" value="SNARE complex subunit SEC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000014">
        <property type="entry name" value="Transport protein sec22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044565">
        <property type="entry name" value="Sec22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45837">
        <property type="entry name" value="VESICLE-TRAFFICKING PROTEIN SEC22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="16">
        <location>
            <begin position="6"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="17">
        <location>
            <begin position="134"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6223220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="215" mass="25002" checksum="73AD9F9D97EA0317" modified="2022-01-19" version="1">MVKSTQIARIDGLLLAATVDDEQAETELADPKANAKMIIRRLNRNSEPQASIEAGKYTLHYLIQDQLCFLTICDHSYPRKLAFTYLSDLATEFTTTYSSSQYLSPTCRPYAFVEFDTFMQRTKKTYQDSRASQNLDKLNDELRDVTKVMTKNIEDLLYRGDSLERMGEMSGRLKEDSKKYRRAAVRINWELLLKQYGPFVGVGVMMIVLIWWRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD09</accession>
    <name>A0A8H6FD09_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nucleoside transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010561</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153047.1">
        <property type="nucleotide sequence ID" value="XM_037301413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD09"/>
    <dbReference type="GeneID" id="59338951"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015851">
        <property type="term" value="P:nucleobase transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000002">
        <property type="entry name" value="Purine-cytosine permease fcyB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF8">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G03000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6223987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="58289" checksum="51C564B36D5DC079" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD79</accession>
    <name>A0A8H6FD79_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152780.1">
        <property type="nucleotide sequence ID" value="XM_037291345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD79"/>
    <dbReference type="GeneID" id="59328825"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="64" mass="6509" checksum="EF0087E3CFE7BFF2" modified="2022-01-19" version="1">MGCAGMLRNSVTVTGPPVAEGAAATVSEELEESVEEAEAAAAAVEAGEVEEEDMEDAAVIRKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEC3</accession>
    <name>A0A8H6FEC3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SMP-30/Gluconolactonase/LRE-like region domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">HO133_010140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153813.1">
        <property type="nucleotide sequence ID" value="XM_037300996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEC3"/>
    <dbReference type="GeneID" id="59338532"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052988">
        <property type="entry name" value="Oryzine_lactonohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47064">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08990)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47064:SF2">
        <property type="entry name" value="SMP-30_GLUCONOLACTONASE_LRE-LIKE REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="2">
        <location>
            <begin position="205"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="437" mass="48785" checksum="508B289F710F7CA4" modified="2022-01-19" version="1">MSHLWHRPKTSDGNLLTPGHKVSFSDSSKSSNKRDSGRPSTASNSRPSSPAAPQPRAASQQRRSSSTRASSQQIWGDNRNFQRINRQSEALWQSTLVSPTAPQPKPFLIYHDSFLPLLGPNPTLQLLIHDPRAPYFHEAGVFHPPTNTLFITSNQLEDSSPDAVTTRNKTIVITKIEFFSPTDFTRDKVRCPERNYMANGGTNYRDGLIICAQGSLREPSGLIYMEAKRPHKTTTLLNNYHSRPFNSPNDVVVHSDGSIWFTDPIYGYEQGFRPKPQLPQLVYRFDPSTGDIRVVADGFGRPNGICFCPDETTVYITDTDLIHGDGNTDYRRAATIYAYSVLWEHDSPYLTNRRVFAYADTGAPDGIKCDVFGNVYSGCGDGINIWNPAGMLIGKIQIEGGCANFCFGRNGEMWCFGEFKLWRVKMGDETRGALLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEJ4</accession>
    <name>A0A8H6FEJ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nonsense-mediated mRNA decay factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009485</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in nonsense-mediated mRNA decay.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154194.1">
        <property type="nucleotide sequence ID" value="XM_037300346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEJ4"/>
    <dbReference type="GeneID" id="59337880"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000202">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.7, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF0">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN EST1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="773"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="888"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="967"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="262"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="912"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="937"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1030" mass="114804" checksum="22CD4779230DF68E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEU0</accession>
    <name>A0A8H6FEU0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154601.1">
        <property type="nucleotide sequence ID" value="XM_037300753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEU0"/>
    <dbReference type="GeneID" id="59338289"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="456"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="621" mass="69744" checksum="C1344B7F3BCBBE29" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF55</accession>
    <name>A0A8H6FF55_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154623.1">
        <property type="nucleotide sequence ID" value="XM_037299797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF55"/>
    <dbReference type="GeneID" id="59337329"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="154" mass="17081" checksum="E93759A60E265D29" modified="2022-01-19" version="1">MMEESCIEAFVPHVSQVPTIDDSRGMVEQSNRIAAARKRKYPGWDARAVHGDLIDRCSRGQTIDPRITDPNGEYQSFDLSRTLQVPLDQRCSQLLSTSSSAVSNTIVDIEKFFGCTNPASEALLEGLLDDMKETGCGSREVVAALKEIEMKTWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF71</accession>
    <name>A0A8H6FF71_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154357.1">
        <property type="nucleotide sequence ID" value="XM_037300509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF71"/>
    <dbReference type="GeneID" id="59338043"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1009"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1009" mass="112822" checksum="6B4FD6BF5D5ADC62" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFC8</accession>
    <name>A0A8H6FFC8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154624.1">
        <property type="nucleotide sequence ID" value="XM_037299798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFC8"/>
    <dbReference type="GeneID" id="59337330"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="246" mass="28211" checksum="4220B6E2E31EFB31" modified="2022-01-19" version="1">MTADSEWLYGGPIENKPALEISPPIKKHLQTTFHEINFKFTEVKNIVDEMATSGVVFKIPTVFKMQLDKVKEAVDELHKVFAMDVYEDSTSTAHKSNLVHVTTIPFRCIRKLFLAPTPEKEYTYITSLFAFRTSFRTFFTDSCKFSPKPKLQNTMSRSDNPSLGDTDPEGIRSAEQDEVQRGREICGALSKADFELRKAEDLYYAYKDLGLSYSTMHASDVKKEFEFAKDYMYLLIQKLFGNSERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG00</accession>
    <name>A0A8H6FG00_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription initiation factor TFIID subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009325</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155012.1">
        <property type="nucleotide sequence ID" value="XM_037300186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG00"/>
    <dbReference type="GeneID" id="59337720"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07979">
        <property type="entry name" value="HFD_TAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000069">
        <property type="entry name" value="Transcription initiation factor TFIID subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003162">
        <property type="entry name" value="TFIID-31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051431">
        <property type="entry name" value="TFIID_subunit_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068">
        <property type="entry name" value="TAF9 RNA POLYMERASE II, TATA BOX-BINDING PROTEIN (TBP)-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068:SF4">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02291">
        <property type="entry name" value="TFIID-31kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="185"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="26139" checksum="D277451A8AD9A60C" modified="2022-01-19" version="1">MAEANGAVPDANPAANQSSSQDEPTAADLINPSFTTNSPVDNGQSKRPRDARLIHLVLSNYGVNAYQERVPLQLMDFAYRYTSSTLQDALHFVSEGYGTSGSGTGSGKAAAQNELSSVTLSALRLSIASRTHYQLNPTLPKEFYNEIAQEKNRVALPPVSKDWGMRLPPEQYCLTGAAWNLREEWDEEDDEMEDAIKEERMEDVDGEGAEGDDGGDEEGGGRMEDIFGDGNNAEAGGDMEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHC3</accession>
    <name>A0A8H6FHC3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_007901</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156105.1">
        <property type="nucleotide sequence ID" value="XM_037298769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHC3"/>
    <dbReference type="GeneID" id="59336298"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000145">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF118">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="141"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="425"/>
            <end position="601"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="96"/>
            <end position="125"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="332"/>
            <end position="391"/>
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            <begin position="1"/>
            <end position="10"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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            <begin position="41"/>
            <end position="50"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6228171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="857" mass="94581" checksum="FD4F470B5A3EE3E7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHJ2</accession>
    <name>A0A8H6FHJ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037298721.1"/>
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    <dbReference type="GeneID" id="59336248"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="113"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="205" mass="23223" checksum="92ED0A45D516A014" modified="2022-01-19" version="1">MSNKLFEQEEDRLLSTEDRRTTASIKPEPIDWEPQYTEGPSRIVSQFDGTKDCLALLLTEEVVQNIKKINRQNRAAERTGRVVDAAQTRTLVLAKRLDGKEIILDGAEDRGEADQIRLEVEQLRSELNEAEEQRDDFKNELESLKLTLECPRKDLQVTLKQKGSKSTDSGHNSIISNSSKSIDPGSEELLRLGAEGNMRQLPDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHR7</accession>
    <name>A0A8H6FHR7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008093</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156297.1">
        <property type="nucleotide sequence ID" value="XM_037298961.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336490"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004470">
        <property type="term" value="F:malic enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000007">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000182">
        <property type="entry name" value="NAD-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053268">
        <property type="entry name" value="Woronin_anchor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40641:SF2">
        <property type="entry name" value="INVOLUCRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40641">
        <property type="entry name" value="INVOLUCRIN REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_2G08060)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="8">
        <location>
            <begin position="66"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="7">
        <location>
            <begin position="254"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="534"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="678"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="941"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1084"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1156"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1344"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1407"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1546"/>
            <end position="1708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1721"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1874"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2017"/>
            <end position="2601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2665"/>
            <end position="2689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2719"/>
            <end position="2795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2809"/>
            <end position="3005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3017"/>
            <end position="3036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3046"/>
            <end position="3341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3364"/>
            <end position="3660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3703"/>
            <end position="3963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3994"/>
            <end position="4017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="4050"/>
            <end position="4362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="4391"/>
            <end position="4413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="4426"/>
            <end position="4787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="4802"/>
            <end position="4881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="4925"/>
            <end position="5283"/>
        </location>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="354" mass="37930" checksum="A6780E091F2E976D" modified="2022-01-19" version="1">MFGALNRFISRLDSDAPSQTSRDSYGAFGFQVLRNKNNDIPTEPWYDFVVGINGRQIEEPDTNSFAVEIRNCAGSNVALTIWSAKGQRARTLQVAVPTPSPTLGLTLQWTPLSSTEDVWHVLDVIPDSPADIAGLLPYGDYIVGTPEGNMRGEAGLGELVEDYLSRTLRLWVYNHEYAVTRLVTITPSRSWGGSGALGCVLGFGALHRLPAPLNEPPAGPGETLFETARFSNEENRPPSAKLRSYPSGSSLYKTSSNQQSSDFLVPATLTTPPPPPPTGATTTGPPRAARKARKAVSPNSAFDEYFKEGEQKSKEEDSTPAAKSAPPPPPKMGGPPPAISPEPQAEAQADKAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHU2</accession>
    <name>A0A8H6FHU2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Man(5)GlcNAc(2)-PP-dolichol translocation protein RFT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008183</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Intramembrane glycolipid transporter that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. RFT1 could mediate the translocation of the cytosolically oriented intermediate DolPP-GlcNAc2Man5, produced by ALG11, into the ER lumen where dolichol-linked oligosaccharides assembly continues. However, the intramembrane lipid transporter activity could not be confirmed in vitro.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the RFT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156387.1">
        <property type="nucleotide sequence ID" value="XM_037299051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHU2"/>
    <dbReference type="GeneID" id="59336580"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034203">
        <property type="term" value="P:glycolipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007594">
        <property type="entry name" value="RFT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MULTISPAN TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117:SF5">
        <property type="entry name" value="PROTEIN RFT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04506">
        <property type="entry name" value="Rft-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="448"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6228453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="595" mass="64781" checksum="CA63C4144A995EE5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI44</accession>
    <name>A0A8H6FI44_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156591.1">
        <property type="nucleotide sequence ID" value="XM_037297956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI44"/>
    <dbReference type="GeneID" id="59335461"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180:SF66">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="338" mass="35612" checksum="4E146AC39E020873" modified="2022-01-19" version="1">MSSDKDQQLLNALKNPSMTSVSKTRLQALSEQLVVKPADPGTFEDIPRIRRIAGDSAGPRVKDKVVIITGTNSPLGIGRASAHQFAHNGARAIYICDFSDQHLSVHVRELQSLYPAVEVHARQFDAGDEAAVAAVVDECLSRYGRLDIFFANAGAVGTNKHFGEVGGEEFMKTMRTNVLSVFVAIKYAAAAMMRTTTSEEGEGGKKKFPCGSIIATSSVAGLRSNAGSTDYSASKAAVRSLVQTTAYQLSGTGIRVNAIMPGLIETGMTAPLFTMARARGTEGKVGQLNPLRRGAVADEVARVALFLGSDEASYVNGQGWAVDGGLSAGHPVVPGKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI73</accession>
    <name>A0A8H6FI73_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Ubiquitin-activating enzyme E1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_007100</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 9">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156628.1">
        <property type="nucleotide sequence ID" value="XM_037297993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI73"/>
    <dbReference type="GeneID" id="59335500"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01489">
        <property type="entry name" value="Uba2_SUMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000618">
        <property type="entry name" value="SUMO-activating enzyme subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.520:FF:000003">
        <property type="entry name" value="Ubiquitin-activating enzyme E1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.20:FF:000005">
        <property type="entry name" value="Ubiquitin-activating enzyme E1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000004">
        <property type="entry name" value="Ubiquitin-activating enzyme E1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.20">
        <property type="entry name" value="Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
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        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
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        <property type="entry name" value="UAE_UbL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030661">
        <property type="entry name" value="Uba2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF5">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14732">
        <property type="entry name" value="UAE_UbL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039133">
        <property type="entry name" value="SUMO_E1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6 9 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="15">
        <location>
            <begin position="17"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-activating enzyme SCCH" evidence="16">
        <location>
            <begin position="182"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-activating enzyme SCCH" evidence="16">
        <location>
            <begin position="311"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin/SUMO-activating enzyme ubiquitin-like" evidence="17">
        <location>
            <begin position="443"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="543"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10 13">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="27"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="120"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR039133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF10585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF14732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6228986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="619" mass="68573" checksum="FCD339CB8683D411" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FII3</accession>
    <name>A0A8H6FII3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156806.1">
        <property type="nucleotide sequence ID" value="XM_037298171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FII3"/>
    <dbReference type="GeneID" id="59335679"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="191" mass="22098" checksum="2D676061D98979A2" modified="2022-01-19" version="1">MSLESPPDEILLQVLKDLNPPGAWDDLAAYSLADETLHPPHSYQTPGWKPSRCVSYTEFNRSCQYWAALRKADLVMQGISASSHHMREVSWDILVLRAMGTSLLSSTTQASAGCLTWKLISRAVDEREEFYGRLLEPIFEDEEEEMRKRARGELWWGFLRDPRITGWLTSGRKMTRKETMASISYLNHRFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ49</accession>
    <name>A0A8H6FJ49_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007559</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157085.1">
        <property type="nucleotide sequence ID" value="XM_037298450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ49"/>
    <dbReference type="GeneID" id="59335958"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189:SF50">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="367"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="396"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="429"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="650"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="650"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="686" mass="75565" checksum="6D9AD922B75BDFB5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK59</accession>
    <name>A0A8H6FK59_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004339</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157258.1">
        <property type="nucleotide sequence ID" value="XM_037295257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK59"/>
    <dbReference type="GeneID" id="59332747"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12710:FF:000002">
        <property type="entry name" value="Protein arginine methyltransferase NDUFAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF5">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6230001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="499" mass="58475" checksum="1FCC92DB83A4CF14" modified="2022-01-19" version="1">MDSPLASKIFQRLLSHQTCSRLRFYPPTSRRLLLNAPGRRYYRSSKEDDGDDDSRQTSDWQMRTDIFPPDKLRDYEKYPMVTSDMLRSRRERPKRVKMLARDFIEDSLYNPHYGYFPKQVVIFSPSQPFDFNKMANEPEFLRQLGHRYTVFEDELDDEVPNESRQLWHTPTELFRPYYGEAIARYLVTNYKLMHYPLDDLIIYELGAGNGTLMLNMLDYIRDFDPDVYARTKFKVIEISSSLAALQSTQLTRNASSRGHTEHVEIINKSIFSWATYVPAPCYFLALEVFDNFAHDSIRYDPFTEQPLQGTVLVDNSGDFYEFYTKQIDPVASRFLQVRDAACSRPFDHPLRESRFTRSLKSRLPFAPNLTLPEYIPTRLMQFFDVLRDYFPKHELLTSDFHALPDAVKGINAPVVQTRYQRRVVPVTTPYVQQGYFDILFPTDFATIEDIYRAITGKLTKVMSHSDFLSRWAYVQDTATKSGENPMLSWYKNSSVMIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK74</accession>
    <name>A0A8H6FK74_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157249.1">
        <property type="nucleotide sequence ID" value="XM_037295248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK74"/>
    <dbReference type="GeneID" id="59332738"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019863">
        <property type="term" value="F:IgE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038903">
        <property type="entry name" value="Allergen_Asp_f_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42039">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_3G02940)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42039:SF1">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_3G02940)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25312">
        <property type="entry name" value="Allergen_Asp_f_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034142045" evidence="2">
        <location>
            <begin position="21"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="379" mass="38832" checksum="FFABD574836DC35C" modified="2022-01-19" version="1">MQLSYQALVLAALSLECVLAHPTHHQLHKHRRDLADLLHQKRTDFSDPATYAPGTFQDLDWASICAGGKCDPSSKSSSPAPANPAPANPAPANPAPANPAPASVPETGSGTVGGSTSSSTPASSPPASTSSASSSGSGSGSDSGSGSGICNDLSSVWTKGDTSRSNAVYMGDGSKCPGGCTSADSTKYSTYGAATLSANSGDAYRGNVGEPYGQNLLPLSDCNTDGHDYSITFAAKSPVTIALWNKVGDDWQTAGRTMTGASRNAWFLFDLAANENAVIGVAANSQVSFSQVCDRNENGQFNCPWGEADFSDTNNGAWSGYDYSVEANNYVNSGGISVTVDGFDVSDSNGCGFYKPGQEDTGCNTNPPAGSMHVKVSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL28</accession>
    <name>A0A8H6FL28_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Linoleate diol synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004842</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157755.1">
        <property type="nucleotide sequence ID" value="XM_037295754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL28"/>
    <dbReference type="GeneID" id="59333248"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20612">
        <property type="entry name" value="CYP_LDS-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09817">
        <property type="entry name" value="linoleate_diol_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050783">
        <property type="entry name" value="Oxylipin_biosynth_metab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034812">
        <property type="entry name" value="Ppo-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903">
        <property type="entry name" value="PROSTAGLANDIN G/H SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903:SF37">
        <property type="entry name" value="PSI-PRODUCING OXYGENASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1 6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1090" mass="121713" checksum="0B6C27B8D6C4539C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYD2</accession>
    <name>A0A8H6KYD2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007223</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156751.1">
        <property type="nucleotide sequence ID" value="XM_037298116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYD2"/>
    <dbReference type="GeneID" id="59335623"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF8">
        <property type="entry name" value="KLAROID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="7">
        <location>
            <begin position="328"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="91"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6229109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="557" mass="60795" checksum="7CBD7DA2A349ACFB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYH3</accession>
    <name>A0A8H6KYH3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156811.1">
        <property type="nucleotide sequence ID" value="XM_037298176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYH3"/>
    <dbReference type="GeneID" id="59335684"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="108" mass="11378" checksum="DB4068E4D57D4ED0" modified="2022-01-19" version="1">MNASVNTSAAAPGNASVAEFLTMAAHQAHGNYGNLTLLWFNATVGAIFIDPSITNVSVLNPAIGDLFAPDIFSVPQACAYPISGQYGFLNRLLYYCLILFAVFAASNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYY0</accession>
    <name>A0A8H6KYY0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA exonuclease 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_007438</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Exoribonuclease involved in ribosome biosynthesis. Involved in the processing of ITS1, the internal transcribed spacer localized between the 18S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156964.1">
        <property type="nucleotide sequence ID" value="XM_037298329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYY0"/>
    <dbReference type="GeneID" id="59335837"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06144">
        <property type="entry name" value="REX4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037431">
        <property type="entry name" value="REX4_DEDDh_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF45">
        <property type="entry name" value="RNA EXONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="11">
        <location>
            <begin position="138"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6229322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="338" mass="37376" checksum="AA714B00417BEF83" modified="2022-01-19" version="1">MINGSGPVMELKDLSTNWKKLQQTLKADTIKSPKRKASESVLQPHRIGVKYRKIESDSIPSRNEKREKSKGMGSRWSSTEDDKPSASLALWAEDHDIPAKDLAAAYGSSLKGTFIPDKNNKTEDTINEGLSPVAEAGKFIAIDCEMVGVGPTPDTDSALARVSIVNYHGHQLYDSFVQPRETVTDYRTFVSGITPQLLQSARTFEAVQADVAKLLDGRILVGHAIKHDLEALLLGHSKRDIRDTSRHPAFKQLAGGKTPGLKKLAREVLGVEIQGGEHSSIEDARATMLLFRREKEGFEKEHIKKFGPDHKRLNGLAEDGGQKGNHSKTKKKKKKTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZQ0</accession>
    <name>A0A8H6KZQ0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157403.1">
        <property type="nucleotide sequence ID" value="XM_037295402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZQ0"/>
    <dbReference type="GeneID" id="59332893"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034134355" evidence="1">
        <location>
            <begin position="27"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="220" mass="23803" checksum="FD3E9D5A06624A33" modified="2022-01-19" version="1">MFIPHHSFSSTTLILTLLQYLHPASSQTNLPSSPTTTSSLPTTSTPVDSPYYNFTYPTFPDDYDSGIQVSYKDTIDVSWIANGVQNTPVLQIQCWTRNDSNYQSRPSYTHNITSSPSPEYPLPLARYRQYSPCELRLLDPHQTSFTNGSESATSVAFFISEDTNASTAGVTWSEDNAAPKVAVDAGVATAASGSAGVYHKGVMALMWAFVGALAAATVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZS9</accession>
    <name>A0A8H6KZS9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004589</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157506.1">
        <property type="nucleotide sequence ID" value="XM_037295505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZS9"/>
    <dbReference type="GeneID" id="59332996"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051799">
        <property type="entry name" value="NADH_flavin_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43656">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G08260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43656:SF2">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G08260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="5">
        <location>
            <begin position="16"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="477" mass="52155" checksum="8CC652DE1DBA636A" modified="2022-01-19" version="1">MGDDSPKPTAIELISQPITLPCGLTLPNRLVKCPMQETLATPPVFDPPIDKFQNLYKQWAHAKYGLIITGQVQIDIRYLSIKGDVVCHKDSFKEPHFSKWKQWATISQEAGIPCIVQLAHPGRMSPAGAGERPPGMAAICPSSVPVELGEGWLNKKALNAVLGTPREATLDDIDAVVAAWVNGARLAKEAGFAGCQLHGAHGFLLSQFLSPHTNRRTDDYGGSPERRLALLKRLVKEIRECCPRPYCLSVKLNSADYMATGLGLSTDEGLEQVRWLVECGEVDLVEISGGNAENKTSKLHNSFGTKSLEKAPKRESTRIREAYFTDFAESVQGLNSRVPIQLSGGFRSRTGMADAIDSGICSLIGLGRAVVLQPDLPVTVLLNPCVSDDEALAVPHIVKGQWFANMIPVKVVGSGLAIQFFYHNMRRLGHGLKSDPDASIPYIVFQGVLETFRSGLFQTVERMLQSFPWQSKASKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L029</accession>
    <name>A0A8H6L029_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004821</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157734.1">
        <property type="nucleotide sequence ID" value="XM_037295733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L029"/>
    <dbReference type="GeneID" id="59333227"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="245" mass="27544" checksum="A84A096AA96C8802" modified="2022-01-19" version="1">MSSNTPASVPVRIVRVSKTSPTDDEDLNRLISLCTATSRSNAFICCLLRQPDSDTSHKQYAHFHYLSVLEDNNSRVFIAHGTIVVGNMDKQRRAAMKRDFYGWLTEKRGNNPVIAGLDVNPAHPKQDQIHKRLVAFAVAEIRKDEPRSDIMAQVATDLEALDQVCPEAEGWKGVEGVLGLSPLILRSLGKQQRTLRVLVNWGLDTILPSTTMSKPILPKKFLDQLYAPDENPVEQFQKMQDLQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C452</accession>
    <name>A0A8H6C452_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000028">
        <property type="protein sequence ID" value="KAF6217440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146875.1">
        <property type="nucleotide sequence ID" value="XM_037297806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C452"/>
    <dbReference type="GeneID" id="59335310"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="201" mass="22384" checksum="CA09DF00AA87386C" modified="2022-01-19" version="1">MAATPPSSGSTTSGTGQLNLLTPQNLSTISLGRATYCKHDDDDPFHFTSSLNNTFPSTPAIPESYLESAGLTTNQETYPFDCFLELPGGVVVFEIKGELDSELTLVFQVWISVLVFNYDEGIYRCNLLEVDKHSCSFTIDKKYINGQAGLFPRDDPDPNFVDLCFEVHGTVKIPYTRVNRRIDFGKCFFKFPKPKTIDADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5T0</accession>
    <name>A0A8H6C5T0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147145.1">
        <property type="nucleotide sequence ID" value="XM_037297435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5T0"/>
    <dbReference type="GeneID" id="59334938"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="29456" checksum="ADCD6222195B96ED" modified="2022-01-19" version="1">MGESSKYHRRLIVQENRQDPARTEPRRVQALERRGPRGHASGREGRTKGVHLVAWEGLPVWASLGFEALERRSRGRRGGVRGRWRRRGRGRGNGASAGDVAETSVNQGGKWVEAEDAGLEDDDGDDVAGARGSGTIGEEDDSAFGTWRANAKRKRARSRRKRERREQFPEPTPRLAGSVYGHVGTGRWPRSGQKGPEKSAGLRATVERGADKVCGSRGAKGKGGLRLRNWKCRAREHDHVRGRIAPTAKGRISRGFMRRPDIIHGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C641</accession>
    <name>A0A8H6C641_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147158.1">
        <property type="nucleotide sequence ID" value="XM_037297448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C641"/>
    <dbReference type="GeneID" id="59334951"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="110" mass="12882" checksum="826D55A629469777" modified="2022-01-19" version="1">MAPSTPNRKAHCADHPIPSQREIDRIIKSVIKAAKRHKFDATKDWRRRFYKAIGKKEDAEFARRPELTFWMACCAMNYDEDPGSRRHWSLSVTALPQIIHKQLAIHDQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6I3</accession>
    <name>A0A8H6C6I3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146976.1">
        <property type="nucleotide sequence ID" value="XM_037297775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6I3"/>
    <dbReference type="GeneID" id="59335279"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="29006" checksum="7F555D98C73FCE9A" modified="2022-01-19" version="1">MPRQSTDDIELGLIINAASHANEASPTSESDPIDPCRLLTLPLELKTQIYGFVLIRSNKVTWQGEYMTEPSPSHAKAQRYICNEPQDPDHLDADGNISALLLTCQTINREASAPFYGDNDFVFKCRTPSPRPRPRLANFPPSSSSNRSHTLASGLPLAFANHVFRANAEHVTFVCAKPDHPLHSCGLEGAMRDDRAGEIGRSCPALKGLVMHEPPVTFWMRSRAERWGLGFVVAVVLALMCGAVGVAVWILVMAVSGGHGASSPTHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8M1</accession>
    <name>A0A8H6C8M1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005557</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148448.1">
        <property type="nucleotide sequence ID" value="XM_037296466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8M1"/>
    <dbReference type="GeneID" id="59333963"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="210" mass="24136" checksum="BF6336E8A8800946" modified="2022-01-19" version="1">MLYVQNVVSQQAWEPIFRLRARPVWVQVIVCDLLRQLKVLRTLTTTLPSLDSVGLPPGRQFRVAFGDMDALCDILEFIHKWYGALQVAYGGPDDRGTLWIKFYKLSAKSRTWETHMQFIIWIDSRRPSALTLEGSNNQFCGPTTVQMPPIDFRVANYQRKIPASQTRRARAGAPGWLGEEEATMVYTRVIHYLPHLPLSECLLITVFSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8X1</accession>
    <name>A0A8H6C8X1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148507.1">
        <property type="nucleotide sequence ID" value="XM_037296525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8X1"/>
    <dbReference type="GeneID" id="59334022"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="615"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="700" mass="77492" checksum="310D06FD736D70DC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C916</accession>
    <name>A0A8H6C916_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">endo-1,3(4)-beta-glucanase</fullName>
            <ecNumber evidence="3">3.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004925</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;3)- or (1-&gt;4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.</text>
            <dbReference type="EC" id="3.2.1.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.6" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148535.1">
        <property type="nucleotide sequence ID" value="XM_037295837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C916"/>
    <dbReference type="GeneID" id="59333331"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02181">
        <property type="entry name" value="GH16_fungal_Lam16A_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000114">
        <property type="entry name" value="Probable endo-1,3(4)-beta-glucanase NFIA_089530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF24">
        <property type="entry name" value="GLYCOSIDASE C21B10.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26113">
        <property type="entry name" value="GH16_XgeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034652732" description="endo-1,3(4)-beta-glucanase" evidence="6">
        <location>
            <begin position="22"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="7">
        <location>
            <begin position="32"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="333" mass="35974" checksum="CE5CA20350B5A401" modified="2022-01-19" version="1">MHSFDLTSLFALTTLLRVSIAGYVLTDNYERDNFFDAFTPFTEADPTAGFVQYVDYAAAMENKMIGAVGNYGNATYMGVDYSSTSDTGRQSVRITSKKAFNKGLFLADIAHMPGGICGVWPAFWLVGPNWPTMGEIDIIEGVNSATTNTMTLHTSSGCSIQKSGMSGTANYTDCDTNAPEQPKNQGCGVLSSDTSNFGAGFNANGGGVYATEWTSDAISIWFFPRTSIPADITASAPDTSKWGTPLANFAGNSCNINAHFQNMNIVFDTTFCGAWAGQVWTQDCKEKADTCEAYVQENASVFVDAYWLVNSVRVYQEGNGGSRKRGLKREVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C977</accession>
    <name>A0A8H6C977_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Plasma membrane proteolipid 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005464</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148356.1">
        <property type="nucleotide sequence ID" value="XM_037296374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C977"/>
    <dbReference type="GeneID" id="59333870"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF42">
        <property type="entry name" value="UPF0057 MEMBRANE PROTEIN ZK632.10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13399" checksum="017FEDCC3C9BFBF4" modified="2022-01-19" version="1">MPNPVNAILVVIVTIFSKRSPFPHRLCPQKPESESLSLTSSSPVVPPVGVFLIAGCGADLLINICLTCLGYFPGHIHAFYLEYVYFNRRDQVRLGNTALKHAPGVYSENIQNGGHTSYGTVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9C4</accession>
    <name>A0A8H6C9C4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148732.1">
        <property type="nucleotide sequence ID" value="XM_037296034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9C4"/>
    <dbReference type="GeneID" id="59333528"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016150">
        <property type="term" value="F:translation release factor activity, codon nonspecific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070126">
        <property type="term" value="P:mitochondrial translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052104">
        <property type="entry name" value="Mito_Release_Factor_mL62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075:SF54">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="104" mass="11733" checksum="C1CD90DB1A4DFB19" modified="2022-01-19" version="1">MHLVPKILHPDIEASRYYAKNSKSLVIQGDGSRDRTSNIQECYKKLHALIIAAGRSSVRGETSPAQVEKVKNLQRKENDIRLLSKKTHGDKKAARKGFSKKSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9M0</accession>
    <name>A0A8H6C9M0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar import and degradation protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005230</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148839.1">
        <property type="nucleotide sequence ID" value="XM_037296141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9M0"/>
    <dbReference type="GeneID" id="59333636"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040979">
        <property type="entry name" value="Vid27_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040768">
        <property type="entry name" value="Vid27_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17748">
        <property type="entry name" value="VID27_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17747">
        <property type="entry name" value="VID27_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vid27 N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Vid27 PH-like" evidence="3">
        <location>
            <begin position="249"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="2">
        <location>
            <begin position="424"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="765"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="788"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="797" mass="89803" checksum="9F85D6F9B33854C0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9R3</accession>
    <name>A0A8H6C9R3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003974</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148940.1">
        <property type="nucleotide sequence ID" value="XM_037294893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9R3"/>
    <dbReference type="GeneID" id="59332383"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37048">
        <property type="entry name" value="QUESTIONABLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37048:SF2">
        <property type="entry name" value="QUESTIONABLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="299" mass="33643" checksum="03B6E5DD45E1F2CA" modified="2022-01-19" version="1">MPTAINVEAEVFIPGQLSYLPKRRDDEPFSLSGSEIPDGCSDHPVVILATDQRERKAVVLILTSFGGKSLNEHSSIPRIRASHLPIHPSPVHPDNGILLFLDQDIELRKMSYVNTESPRQIEWALLQRHYREQRTGERFRLRPDSFAKLLDYIGSEAPLGRGTSVAPQSPLHPSTTYSPGWDLLESMKANSLAQYGSINRPTVPPRRISEPARYLVRAPTATSTLQAVARSREGAAAEVDRYLAQRREARRHETRAILPTHRPSPRSDSSYDPMSILRKLFVAVLVAVGLFFAIAWWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA70</accession>
    <name>A0A8H6CA70_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149305.1">
        <property type="nucleotide sequence ID" value="XM_037294617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA70"/>
    <dbReference type="GeneID" id="59332106"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033925">
        <property type="term" value="F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032979">
        <property type="entry name" value="ENGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005201">
        <property type="entry name" value="TIM_ENGase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246:SF1">
        <property type="entry name" value="CYTOSOLIC ENDO-BETA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246">
        <property type="entry name" value="ENDO BETA N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03644">
        <property type="entry name" value="Glyco_hydro_85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel" evidence="1">
        <location>
            <begin position="116"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="716" mass="80062" checksum="D4337D25313F9DA0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAE7</accession>
    <name>A0A8H6CAE7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003664</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149274.1">
        <property type="nucleotide sequence ID" value="XM_037294586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAE7"/>
    <dbReference type="GeneID" id="59332075"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034695176" evidence="2">
        <location>
            <begin position="16"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="13138" checksum="3255C6D486AF372F" modified="2022-01-19" version="1">MSSFIFLLLWTVVQCLCAATAPSPSYTTFQDQSSPPSPTLNARNSPICVRVAQNPDWAGAIDAKHCADAIGRLWDRTLPQPNSSQMLHPARHGKSPKDQSTVSSMISFGKPRPSSNERDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB76</accession>
    <name>A0A8H6CB76_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149494.1">
        <property type="nucleotide sequence ID" value="XM_037294806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB76"/>
    <dbReference type="GeneID" id="59332295"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034819741" evidence="1">
        <location>
            <begin position="21"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="317" mass="34254" checksum="C792E3F684E798F1" modified="2022-01-19" version="1">MPGISSLVVLLAHCVIVALALFIPLQLPANVNIPSLGLPVNATDLKGQPAVPGFDAVLSWVPRVSINPEDAYLCAIDFMYGLASSGWDEIIPSGYEASSKDVNGIIIAYGSLAHPEDKNQLQNKHLVLGLLKTMNTLASRKSFCITRAVLYMYNKPVGQLAIARQVSPTLTEINGTNFSLGNLDPMTNSMASRNLTVVRDIVDPEDSNFVISFEILEDSIPCQSLLNAAVNGLAISAPAKNDDVCTEFAGVSSGGEVTYMIQRNSPGTTRFLLTYNLVRRSLKLLPARMYKDDLCGEVKFDFIYGDEELGGGTMFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBB6</accession>
    <name>A0A8H6CBB6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149628.1">
        <property type="nucleotide sequence ID" value="XM_037294247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBB6"/>
    <dbReference type="GeneID" id="59331735"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034444">
        <property type="entry name" value="Nuo17.8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42100">
        <property type="entry name" value="OXIDOREDUCTASE 178 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04320)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42100:SF1">
        <property type="entry name" value="OXIDOREDUCTASE 178 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04320)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="126" mass="14715" checksum="03A7A6DA8B824104" modified="2022-01-19" version="1">MAYYNYWQDEYARRNTMHTKMVEQAGADRNLFQGSPWSHHIDLKFPEIFNTGSPYNIPAGHSADLGALRAHYEKKNVEVEEKRIAKLKEMAGEREILQRRKEVEEALLADKKAPSILQWMGLQRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBX3</accession>
    <name>A0A8H6CBX3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150173.1">
        <property type="nucleotide sequence ID" value="XM_037294095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBX3"/>
    <dbReference type="GeneID" id="59331583"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="31764" checksum="73308AAB0768D4FB" modified="2022-01-19" version="1">MIRAMQASRNEPHASHALSKVVTISFYNTQGGRADFTLLTQWAYVHSFQDAEDPVDEDLLARKSNVIALEVTESMINDNDLDTLLCAIKDLHTFKSEYGFPNNSEGFCRASRLGYEEWNIRAVIDILFTHARHSLAVLDSTMTDNQKYLTGIASLAESLLEKLRADTMVFVESEVEKLFITYVLTYLTDEPFVRIMNEAEGYPPAHVRQFVDILPASLEELVLFQTMSAYADAERISDGMLKHKREELPALAKTFWEGLEPQTLGEMWYCKGLDMDVSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC22</accession>
    <name>A0A8H6CC22_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150080.1">
        <property type="nucleotide sequence ID" value="XM_037294002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC22"/>
    <dbReference type="GeneID" id="59331490"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="421" mass="45687" checksum="6F8B45EF6D581CEF" modified="2022-01-19" version="1">MSDLIELLSDLALLESNPLDMDQRAVSLIFNIKREIDEFLANLTMSANLAATSPHNDEYSTVSTSKYTQSTLGTSRLIWDHHRDAALYDGFGSLSQSTDSSTDSMHAPSLQANPGLGVTALYKSSESRFEAMNANCMQVNSYENETHSESRFGIMNGDLMVINALQEGLLIPQLTDTALKRPAATGPYDLSVSHSSASSPTSTGLFPIPFPTRGTTTQNKRVFPGALVASGRTSGANLVPDSATATNFTGPSNSDSALRGNATQMKRALSEASSRIAAGGAQKKAKGDMDPENHEIYRLRQENKQFDEIAEIINNVRSSHGKPSNLTGNAIYGRYKRNATLIAAAKGEVYKPCALDFQAGTNLKVITQPVPAGFDDNEDRLLVEAYKYVMENAWVLVSKRLEDTSGRVHDPVDCANRFAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCG2</accession>
    <name>A0A8H6CCG2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002584</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150339.1">
        <property type="nucleotide sequence ID" value="XM_037293510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCG2"/>
    <dbReference type="GeneID" id="59330997"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013893">
        <property type="entry name" value="RNase_P_Rpp40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15396">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15396:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08584">
        <property type="entry name" value="Ribonuc_P_40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="415" mass="46655" checksum="C04E7EF969500D79" modified="2022-01-19" version="1">MFSFLESKTSASKAYVSHGNLPPFVDPEQPPSTKKPFSTILGQPFTHTVQLILPQELYDVVWKEVEAKISNIQYSRVIMPLSGLLEGDFFNRYIKIGNILMLSEGRPGVDDRYSLKDGKLVPERVLRLELAKDKYERCGLVGQPVRDAGRKHLKTRYAVDINLRLPSMLHGKKGFERIVWALKNVLNHSVTWLFHDLNSNSEPSKAIASAKPDDNCVTECSIAATDAPIIEHHPVTKDCSPHQKTTERVSAPNFIPPPHSDSNDDFEDWALETYEWLSLVAMESPRILSEDTIDPYLSRYQIPDGDSAKVLNMITLTWTGFIPALWARHLFICLSSAKTTIREGASSEDFFALSAYSFKTAVTDQKDAYTILCVSDEIVNSQSAVAVAGKTKESGHSRVYERRGRKYVLWEQSCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDM7</accession>
    <name>A0A8H6CDM7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="1">3.2.1.106</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glucosidase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150776.1">
        <property type="nucleotide sequence ID" value="XM_037293123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDM7"/>
    <dbReference type="GeneID" id="59330610"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031335">
        <property type="entry name" value="Glyco_hydro_63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054491">
        <property type="entry name" value="MGH1-like_GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF10">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 63 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03200">
        <property type="entry name" value="Glyco_hydro_63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22422">
        <property type="entry name" value="MGH1-like_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mannosylglycerate hydrolase MGH1-like glycoside hydrolase" evidence="4">
        <location>
            <begin position="444"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="3">
        <location>
            <begin position="723"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="939"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="973"/>
            <end position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1018" mass="118006" checksum="07AD924D9D8D1B24" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDX2</accession>
    <name>A0A8H6CDX2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001590</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151059.1">
        <property type="nucleotide sequence ID" value="XM_037292520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDX2"/>
    <dbReference type="GeneID" id="59330006"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079:SF6">
        <property type="entry name" value="GH26 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034644839" description="GH26 domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="6">
        <location>
            <begin position="198"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="371"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="642"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="374"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="392"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="436"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="462"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="489"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="534"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="561"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="643"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="678"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="699"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="731" mass="77530" checksum="0110DF6EB7827934" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE78</accession>
    <name>A0A8H6CE78_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151011.1">
        <property type="nucleotide sequence ID" value="XM_037293358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE78"/>
    <dbReference type="GeneID" id="59330845"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021986">
        <property type="entry name" value="Spherulin4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35040:SF9">
        <property type="entry name" value="4-LIKE CELL SURFACE PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14080)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12138">
        <property type="entry name" value="Spherulin4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="261" mass="27884" checksum="6295775A2E586FB0" modified="2022-01-19" version="1">MKSLLLLTPLLLALPTTSLFILLPLYVYPGTSASAWNNATAAIAAYPKVQWQVIVNPNSGPGTTGYPTDQNFITGIAKLNSYPNVQTLGYVDTANTNRAYSALTSDISVYAKWTSYTKANISIAGIFFDDVNNTASSAVNTYMHNASAYAYATVPSDVTPVVFNPGALAPTQLFSYCDTMVEFENPFSSYKNDTTIATIPSAYRDQSAILVYSTPATANVKSLVHTMALDGIEAVYFGADCCYDSFPAAFLTQLAAAVQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CET4</accession>
    <name>A0A8H6CET4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001141</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037292071.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59329557"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057827">
        <property type="entry name" value="WW_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23518">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="550"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="627" mass="67609" checksum="84F9A38F49848993" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF00</accession>
    <name>A0A8H6CF00_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151595.1">
        <property type="nucleotide sequence ID" value="XM_037292176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF00"/>
    <dbReference type="GeneID" id="59329662"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="502" mass="54099" checksum="F0B1F0A27C4AE2BE" modified="2022-01-19" version="1">MAASPPDGDFVYPDPSLFGINMPPPPHSHPYPLTLSSGPNPHAAPAPAPAPIAGPIPAPRLSARGLQPFPALFPGLQAPIELTNVADIAFWDKQLLLSFDHSRIGLRNGQQGIRKHSSVSRRCRARIQLRKLWQSAGGEPYVKRRVFEDNPDLHITREFHHGSYTKTNISTTTGGPPSRSDSTGSARVDPESYDDLAETSQFYSRDQRVEDNVHLATIGFEPVNKSDEALFLWKSYLGAHNLASVNTVGTGLPDALRHRGGARQAVVFLAARALGRTVTVGPPAVTATVGVPQAAGGPPFTTAGGLAESATVGLGGAGPSTAVFRAAPSGPHETPRRENHRTLMTRQVEDAIISAALNNDETLLLALHYHRENLPDRSRESDRTAITRLAVQFLDFARPRASASEALIELARARWDVDAALDSWGSNNGDGVENYDGADDQNNEGQPPKKKQRLHDTDAADEGEEEEEEEEEEEGNGKGKGKGKSKLKGKQKGKGKEKEANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF23</accession>
    <name>A0A8H6CF23_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001132</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151481.1">
        <property type="nucleotide sequence ID" value="XM_037292062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF23"/>
    <dbReference type="GeneID" id="59329548"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF33">
        <property type="entry name" value="PROTEIN HOS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="129"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="162"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="350"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="582" mass="62513" checksum="A6DFA86BBB27FDAB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF37</accession>
    <name>A0A8H6CF37_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">hydroxymethylbilane synthase</fullName>
            <ecNumber evidence="4">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Hydroxymethylbilane synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_001443</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HMBS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151792.1">
        <property type="nucleotide sequence ID" value="XM_037292373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF37"/>
    <dbReference type="GeneID" id="59329859"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000005">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="11">
        <location>
            <begin position="41"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobilinogen deaminase C-terminal" evidence="12">
        <location>
            <begin position="280"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6222357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="423" mass="45369" checksum="30E258243EDF51C6" modified="2022-01-19" version="1">MADLPRTSTNHPSTTSSHPRASPSGSTSTHPASPTSTPRTLRIGTRSSALALAQVALFTDLLRTSHPTLSTTTHPTSTSLGDTNKSTDLHTLASTGKSLWTEDLEQELRERRVDVIVHSLKDVPTKLPPGCVCAAVGAREEARDAVVMSAARRGEGWRGLGDLREGGVVGTCSVRRAAMIRRTYPHLVIKDVRGNVGTRIRKLDDAAGGFDALVLAGAGVQRLGMGERISGWLGSEEGVLYAVGQGAIGVEWREGDEWIEGILAEAEERVGKAGRRVRWECVGERSLLRVLEGGCSVPVGVECVWEEGGLQEGGLQEGDGRGTDAVVEQADKHHNGYEQDDALPEGPDPERKGYGGILIMRAMVVSLDGKECVEGTRRHYVSSDAEAEECGWRMAQELVEKGAGEILKKITLNRDMIRGQDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFW7</accession>
    <name>A0A8H6CFW7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152128.1">
        <property type="nucleotide sequence ID" value="XM_037291766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFW7"/>
    <dbReference type="GeneID" id="59329248"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="470"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="395"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="508" mass="58557" checksum="D6D30DAEFFD519D2" modified="2022-01-19" version="1">MSDFKNRSVSDMQLPNTSSHKRDDDETYRMTVLRFDKTEDEVDRHFLQMALDLGINIPQEPKTTIDHITSNVATLSINSDAPEPHQLSPSRASESTHTASESSIEQMEHTKTPSLTATSVTTASISSAASNKSNYVKVRNGFRRISRLHRRKTMAVPVPAIPLRTSSMFHILRPGLEPRAITADQIATIYNPQPHAITTDQIRTLSIPRKRVMSTTYNAPPPLPPPAPLSPPLRSPGEETIETLYARRRSISNPTLKKLRTTQLQEQLRFISFEASQHRLMRTKQLQHKRDSLSRYQDQERDTHAQHTEALSSLETRHLTAEVDLQRTLQLERQACDTRLKHMQAYCNPRSTVAGMPSRVVTKSDYRQLEQQYHIRNGMDNLHASRINVLRERQGKQQERIMAKQEAELETLESKFEQQNEELDTRFREEEMQLQQEFAERKKRLVRRWALAEAIERRKLEKATGETFEPLPPIFWSGDENGRGKEKERAREVPGKEDNPTYDAMNMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG30</accession>
    <name>A0A8H6CG30_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein YAE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein yae1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_000873</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The complex LTO1:YAE1 may function as a target specific adapter that probably recruits apo-RPLI1 to the cytosolic iron-sulfur protein assembly (CIA) complex machinery. May be required for biogenesis of the large ribosomal subunit and initiation of translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">May form a complex with LTO1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the YAE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152168.1">
        <property type="nucleotide sequence ID" value="XM_037291806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG30"/>
    <dbReference type="GeneID" id="59329291"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019191">
        <property type="entry name" value="Essential_protein_Yae1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038881">
        <property type="entry name" value="Yae1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829:SF0">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09811">
        <property type="entry name" value="Yae1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Essential protein Yae1 N-terminal" evidence="11">
        <location>
            <begin position="52"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6222822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="196" mass="21456" checksum="D863B1ECF533BD62" modified="2022-01-19" version="1">MALTPPSTPPSPSSPTINNNLSDIFSDSSPTSPTTADPSDIPRLRSTHSTAGYRAGISASKQRSLQPGFDEGYSLGAVFGLRVGYILGALEGLCAAHSQESVDRKGLGQLVKEAREHLAIEKIFGREWWGEDGIWRYQVQGKGEEVTFRDVVDAHPLVRSWVERVDQEMKVAGVKVGRFEGREWEEGRMDKEAGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGI7</accession>
    <name>A0A8H6CGI7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000837</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152135.1">
        <property type="nucleotide sequence ID" value="XM_037291773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGI7"/>
    <dbReference type="GeneID" id="59329255"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="193"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="401"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="545"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="7">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="546"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="560"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="576"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6222789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="608" mass="66652" checksum="252B2E2E59241205" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGQ8</accession>
    <name>A0A8H6CGQ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152240.1">
        <property type="nucleotide sequence ID" value="XM_037291878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGQ8"/>
    <dbReference type="GeneID" id="59329363"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="363" mass="39970" checksum="723C2BF9BC37E550" modified="2022-01-19" version="1">MIREADARKQKLHDILQIVDVGILQLWARGNPCQIDYLAIVVLEGLELWPFTIDVLTVLASIPSFRNAALQQKPMLFETLSTNAIDSNSGFDKYAATCVALLSTSSELPIPSKVPIFLMKCLEKATQSLSPETIRPIYLLLSGLESSYLDILPFDVLSSFQSQMVEVLTNLDSDNHLASLLCLAILAKIASRSSGMEKSQPSIQSSPSGEVRPESADRFLPARKFFSSRRAPKTLDLVVLKAITAFSRHCPLSTQEIVESLKLSREIVDAFDKKEKSSWLAANTRKTKKLYEKILTSGVEARVQCEALNFLVVLSAGESLPPEVIPNLKKHLLASETDNLSASAIGQCIVRPFIAQEKQAVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH56</accession>
    <name>A0A8H6CH56_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152702.1">
        <property type="nucleotide sequence ID" value="XM_037291267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH56"/>
    <dbReference type="GeneID" id="59328747"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF34">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SETD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="209" mass="23786" checksum="9458F4B9399BBE29" modified="2022-01-19" version="1">MATYASNDTFHPKTADFLKWLRERPGTTISRKLEIIDLRDHNAGRGIGNRLSPESLVSILAESPVVAVEDVEEDEELFRIAQSDVLTVQNSSLQKVKPHLLERLDPWNSLVLVMIYEDGLGKESKWWNYLQLLPTNFDTLIYWSLSELAELEGSAVLNKIKKMTLTSLSKSFYCQSCNSSLRFLVGMPMTLLVPTHPPFYSTLLTEWPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHA5</accession>
    <name>A0A8H6CHA5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152458.1">
        <property type="nucleotide sequence ID" value="XM_037291023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHA5"/>
    <dbReference type="GeneID" id="59328502"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="314" mass="34980" checksum="D63430DF15DC127B" modified="2022-01-19" version="1">MSITPTGSTNPIGTLSLLPPELRDQIYDHVLDASYVVFWTSHQHDGHASPDGVNPTVSADLEILRVSKALGAEAGARLFSHATVFAYLIRFDGHDRLSRPPPKGATEKMMNVEFVIDTGAPLEGEYVGMMEDGIEFYGRSAWGVDMSPVSMDDGKVRRPPTVYRPAAMDPRCEASVDHFTGTKIERNNLLVTFRDFDENFHLFTATRFFRTLKECVGFRTVTVGLEWWQLDGWVANTAAVAEKVEGLRVELEQCWGPCVVRDVFDQYTETTAGPDMKLCFAFELAFQPRKYRGENLKVGAVGVMKGGDRAEEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHT3</accession>
    <name>A0A8H6CHT3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000339</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG101 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152713.1">
        <property type="nucleotide sequence ID" value="XM_037291278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHT3"/>
    <dbReference type="GeneID" id="59328758"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012445">
        <property type="entry name" value="ATG101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292:SF0">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07855">
        <property type="entry name" value="ATG101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="197" mass="22214" checksum="5A932C4C1A420295" modified="2022-01-19" version="1">MEQRKPPQYILEVFADPTCVKDIVRGILHTIFFHRYFPSIRPSSIEVLDLTLPLVADPELETLIDTRVGQLIRQLSSTSSPKSSVRGSLVVQFFEKKRRKGGGLGWFTGGAKGDEEVCWESWCLEVTLATPKTEMERTKVMKAIGSTLQKTAMKIITLVNRDKDHIPPITTSETNPFPFHIELNPRVEGWGKGIGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI78</accession>
    <name>A0A8H6CI78_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010553</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153039.1">
        <property type="nucleotide sequence ID" value="XM_037301405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI78"/>
    <dbReference type="GeneID" id="59338943"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000069">
        <property type="entry name" value="ER associated DnaJ chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF3">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="50"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="359" mass="39720" checksum="28805580DEBC1872" modified="2022-01-19" version="1">MPAATASGADATPNGNAAKSRDHNQGNQERKYTVEQKAAVIRVRKCSATAFYEILGLEKAASDAEIKKAYRKLSLLTHPDKNGYDGADEAFKMVSRAFQVLSDAEKKEKYDRFGGDPDNRFGPGAAAPSSPFSGFAARSPGGRGPMFEDEISPEELFNRFFGGGGMGGGPFGGGVPMFNDGPQYVFNLGGGPGFRVHQFGGNRPRRRPREANGTGEEQPRSTLGALSNLLPLLILFVLPILSSLFSSAMPSGPNIHFQPGPPNTMHRTSQRLRIDYYLDPRDVVDYSGRKMNDLDRQAENQFVTQLQYECQIEQRTRNTMIEQAQGWFFQDADKMREARNFDMRSCKQLDTYGLARNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIE3</accession>
    <name>A0A8H6CIE3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010631</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153117.1">
        <property type="nucleotide sequence ID" value="XM_037301483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIE3"/>
    <dbReference type="GeneID" id="59339021"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019459">
        <property type="entry name" value="GRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921">
        <property type="entry name" value="MYOSIN HEAVY CHAIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921:SF2">
        <property type="entry name" value="THYROID RECEPTOR-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10375">
        <property type="entry name" value="GRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="6">
        <location>
            <begin position="442"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="491"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="544"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="135"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="308"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="551"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="564" mass="63099" checksum="206628383FC1A204" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIJ9</accession>
    <name>A0A8H6CIJ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear segregation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010808</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153293.1">
        <property type="nucleotide sequence ID" value="XM_037301659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIJ9"/>
    <dbReference type="GeneID" id="59339198"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042175">
        <property type="term" value="C:nuclear outer membrane-endoplasmic reticulum membrane network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039604">
        <property type="entry name" value="Bfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027:SF2">
        <property type="entry name" value="LEBERCILIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027">
        <property type="entry name" value="NUCLEAR SEGREGATION PROTEIN BFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="324"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="416"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="177"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="429"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="472"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="485"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="58442" checksum="A3A44D8E33519D19" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIM8</accession>
    <name>A0A8H6CIM8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">sphingolipid 4-desaturase</fullName>
            <ecNumber evidence="3">1.14.19.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010639</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase type 1 family. DEGS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.17" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153125.1">
        <property type="nucleotide sequence ID" value="XM_037301491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIM8"/>
    <dbReference type="GeneID" id="59339029"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03508">
        <property type="entry name" value="Delta4-sphingolipid-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000012">
        <property type="entry name" value="Vacuolar membrane PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011388">
        <property type="entry name" value="DES1/DES2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013866">
        <property type="entry name" value="Sphingolipid_d4-desaturase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879">
        <property type="entry name" value="SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879:SF8">
        <property type="entry name" value="SPHINGOLIPID DELTA(4)-DESATURASE DES1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08557">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01269">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="343"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingolipid delta4-desaturase N-terminal" evidence="11">
        <location>
            <begin position="303"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="632"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="632"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="654" mass="72972" checksum="CE7250A0C9FD3F67" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ46</accession>
    <name>A0A8H6CJ46_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010713</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153199.1">
        <property type="nucleotide sequence ID" value="XM_037301565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ46"/>
    <dbReference type="GeneID" id="59339103"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000006">
        <property type="entry name" value="Endo-1,3-beta-glucanase Engl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034782904" description="glucan endo-1,3-beta-D-glucosidase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="10">
        <location>
            <begin position="121"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="11">
        <location>
            <begin position="452"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="812" mass="86617" checksum="CB67706B84EFB73C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKU4</accession>
    <name>A0A8H6CKU4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peroxisomal adenine nucleotide transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_010387</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154057.1">
        <property type="nucleotide sequence ID" value="XM_037301240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKU4"/>
    <dbReference type="GeneID" id="59338778"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF2">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G13870)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="34"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="142"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="249"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="491"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6225190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="56102" checksum="13027C276C7D3B35" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKW0</accession>
    <name>A0A8H6CKW0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009510</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154219.1">
        <property type="nucleotide sequence ID" value="XM_037300371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKW0"/>
    <dbReference type="GeneID" id="59337905"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034477581" description="WSC domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="4">
        <location>
            <begin position="32"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="419" mass="43731" checksum="A6D4CC6E293D3522" modified="2022-01-19" version="1">MATPIWRRLGARPFIQSLAILLAFAAPRPSVQLSQELCSSQNTGSDSNAVSNHFNSNGACQTACSGFAFAVVQNQNCWCSNYSPADTTSTGNCSEACPGYPYENCGSASSGLFGYVALGNAPSGTIGASTSTSTTSTPTPTPTTTPTPTPTLAPTPTTSDTLQATPSTTSTSSTSQPIPVSLNLTPTTTSTPTTLTTSSTPTTIAKSSTTTSSSSWSATPFTSVTTVTGQIKTLTITPTSPPATATTIITAAKENNGGFFSSSGKVAGTFVAVSIVLLLLIASVIWFFLRRSRRRRPEPAAISSVAGSSTPQRRPSRLSQMGLVGAGGPQREKTGPTIPVIQTSGWGPSNRDGSEKSPADTSSAVERRSSYPRVVDQRLEPTALWNPLHDNGSHVSVRSFRDDQDYSRRMLRIANPDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL51</accession>
    <name>A0A8H6CL51_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010388</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3RF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154058.1">
        <property type="nucleotide sequence ID" value="XM_037301241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL51"/>
    <dbReference type="GeneID" id="59338779"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052256">
        <property type="entry name" value="E3_ubiquitin-ligase_CHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16079:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16079">
        <property type="entry name" value="UBIQUITIN LIGASE PROTEIN CHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="19"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="844"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="94"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="148"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="270"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="446"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6225191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="963" mass="107171" checksum="94919B26EB06465E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL97</accession>
    <name>A0A8H6CL97_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154171.1">
        <property type="nucleotide sequence ID" value="XM_037300323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL97"/>
    <dbReference type="GeneID" id="59337857"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="196" mass="21763" checksum="72681E0FA66C0FD0" modified="2022-01-19" version="1">MPSKPKPLKPPRHTPKVVAIILCFSILRVNAKVVSELIKLKNPGTVEIEAIECRARRGFHERSDFPNRGMTKAGVGELVARKVVPDYDEIIAITEIDGECEKHSDIPLAELKDGIKEIRAAAMKTLNIGCDYDIGQDFIQGNQPRSPSQDFKEYVAKANFAPYRHGSKNKPSPSQAFKKWSFDPVLGRESPSPEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM34</accession>
    <name>A0A8H6CM34_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UFSP1/2/DUB catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009884</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154591.1">
        <property type="nucleotide sequence ID" value="XM_037300743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM34"/>
    <dbReference type="GeneID" id="59338279"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012462">
        <property type="entry name" value="UFSP1/2_DUB_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07910">
        <property type="entry name" value="Peptidase_C78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UFSP1/2/DUB catalytic" evidence="2">
        <location>
            <begin position="107"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="334" mass="37381" checksum="2BE4164AB840143F" modified="2022-01-19" version="1">MPPAAPDRTLSGLVGGCFSIVSGKRNDAENGGRLGKRDLGRYAYENKMPDWLYCLLEDEGKQHLEDAPSGISDVLGKLIDQDRDVETAYICHFAVRYVGKIKVKGKNEGNNFCGYHNIQMLVSYINAAEVQGSENFANGIPTIWEIQNIIEEAWDHGFNESGRLQTGGIKGTRKHIGTPEAQALFGSLKIPCKTDRFQDIKGGAKAYEQLLDSVEYYFSNSITTSHLRTSPRVQNTELPPIYLQRPRHSMTVVGIEKRKNGSRSLLVFDPAYNPSKEMLKMLNLASATNSDTPSISLIKPYRRGKRYLKRYQAFETLRLVLQPSLSGQRTNQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM43</accession>
    <name>A0A8H6CM43_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase family 34 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154606.1">
        <property type="nucleotide sequence ID" value="XM_037300758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM43"/>
    <dbReference type="GeneID" id="59338294"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF8">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 34 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="42846" checksum="347B136B6C9EC8D0" modified="2022-01-19" version="1">MAARRRPLFFIAVPALVFIYFFSFTGKHSGPFTYPPPIPPSESLGTHAGLENCTDDFDASYEGLRVRQATMLYEGDKDKLRGIYERCVETHRKHGDRWGVPTHVLSHALYEDNSYFNKPAYLQSLIMVELAKPAAKRADWIVWFDGDSVIMNPEIPWTIFLPPSDLHDYHILGTQDNNGFNAGMLILRIDQWTVRVLSQVLALPVLQPSVGLPFYDQSALDWVFQLPANQEHFIFQPRHWWNRYFFTGDGDQPGEMLLHFAGIGAQSGGGSQEKSAVMRKYLERAETDPQSWTKDLSKTPYEKETKAFWDDVRDAKETLQKTGKWFRDHPANKHVREVQEAEKVLRETLLREADKVDWLSGNTTQLQKLLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM56</accession>
    <name>A0A8H6CM56_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">General negative regulator of transcription subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_009806</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154513.1">
        <property type="nucleotide sequence ID" value="XM_037300665.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338201"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000012">
        <property type="entry name" value="Ccr4-Not transcription complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25097">
        <property type="entry name" value="ARM_Cnot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="13">
        <location>
            <begin position="664"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="12">
        <location>
            <begin position="842"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="11">
        <location>
            <begin position="1056"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="10">
        <location>
            <begin position="1338"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="14">
        <location>
            <begin position="1646"/>
            <end position="1779"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="9">
        <location>
            <begin position="1956"/>
            <end position="2314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1558"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="106"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6225804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2334" mass="261521" checksum="C15C7CD63E6DC6A6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMK8</accession>
    <name>A0A8H6CMK8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009033</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154720.1">
        <property type="nucleotide sequence ID" value="XM_037299894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMK8"/>
    <dbReference type="GeneID" id="59337428"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000118">
        <property type="entry name" value="40S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="115"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16777" checksum="052C525BCD51A5DF" modified="2022-01-19" version="1">MTGGVSVRDVDAQQFITAYSAFLKRQGKLPIPGWVDTVKTSPSKELPPQSIDWFYTRAAAVARHIYLRKTVGVGRMRKVHGSTKNRGSRPSHHVDASGSVDRKVMQALENIGVLEKDEERGGRRITQSGQRDLDRIAQTTLEESDEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN10</accession>
    <name>A0A8H6CN10_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arrestin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154958.1">
        <property type="nucleotide sequence ID" value="XM_037300132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN10"/>
    <dbReference type="GeneID" id="59337666"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="60824" checksum="BA1939C83F91C29E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP00</accession>
    <name>A0A8H6CP00_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155498.1">
        <property type="nucleotide sequence ID" value="XM_037299497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP00"/>
    <dbReference type="GeneID" id="59337027"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="692" mass="75399" checksum="10D8E52260A511C8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQG9</accession>
    <name>A0A8H6CQG9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_008830</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fungal Na(+)/H(+) exchanger family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155694.1">
        <property type="nucleotide sequence ID" value="XM_037299693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQG9"/>
    <dbReference type="GeneID" id="59337225"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000015">
        <property type="entry name" value="Na(+)/H(+) antiporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF2">
        <property type="entry name" value="CATION_H+ EXCHANGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="27"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="453"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6227386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="485" mass="53147" checksum="B33B43F50FE1DD2A" modified="2022-01-19" version="1">MSWISKQLELTPTHFVYLILSAFLLLYALFSAFIRNRLHLSEPPLATLVGIAFGPQGANLISSVRWFSDDITQEAARVVVGLQCFTVGVELPKAYFSRHWKSVGMMLGPVMTFSWLITAGFVYAVLQTKVTTALIISACLAPTDPVLAASVLAESTFSKRVPRRLRHMLSAESGCNDGVSFPFLYIGILFLVEQTPGAAVKEWVLGTVLWQCTLGLLIGLVIGHSFNRSLRFAERWAYISPAPFLVFYFLLAIFSVGIGSTLGVDDFLVAFGAGYGFAWDGWFAKKTRETNLPEILDLLLNSSMFVYFGAIIPWRSFTPTEFTPSITPGRLVGLLILILLFRRIPIVLAMKRFVPDIRTYREALFCGYFGPMGVGALFLVIEARAMLENGTSIPDAHPPAENENKQAIETVWPVVCFIVLGSTMVHGLSVAAISVGSHYARKEGERAPLIGGESDGLYGMVHEGGGGESEPSVSGDEDPDPPDHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR37</accession>
    <name>A0A8H6CR37_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155868.1">
        <property type="nucleotide sequence ID" value="XM_037298532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR37"/>
    <dbReference type="GeneID" id="59336059"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034857401" evidence="1">
        <location>
            <begin position="24"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="162" mass="17270" checksum="C02DC3B41F6ADBCC" modified="2022-01-19" version="1">MNLLSLFSVILLSLLVTITSTQSTNLTTPSNTANFECFPLRVFALRVGLFQNCAAAILTLPQLTAPVSFYHGYRDDIGRLPTTRSHGDCMVTVDLIESSEFSTWTSIGLVATLLMSACADISLNRVYTGGFAKAGNGRGIAVTLQRFEEGLGAGNASGLLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRB5</accession>
    <name>A0A8H6CRB5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007888</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156092.1">
        <property type="nucleotide sequence ID" value="XM_037298756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRB5"/>
    <dbReference type="GeneID" id="59336285"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017149">
        <property type="entry name" value="GSH_degradosome_Dug2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF8">
        <property type="entry name" value="DI- AND TRIPEPTIDASE DUG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037237">
        <property type="entry name" value="Peptidase_WD_repeats_DUG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="88"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="244"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="291"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="359"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="7">
        <location>
            <begin position="594"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="796"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="811"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="835"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="918" mass="101156" checksum="9C65CC6CEE8C1AD9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRH4</accession>
    <name>A0A8H6CRH4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007978</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156182.1">
        <property type="nucleotide sequence ID" value="XM_037298846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRH4"/>
    <dbReference type="GeneID" id="59336375"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF7">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="13"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6228248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="452" mass="49833" checksum="EF3639556D8189B2" modified="2022-01-19" version="1">MALSRFIIELRINLSPEARVYTDASSKDFQLALLRWSDVDVKVPGAIIQVANELDAVTTASSTPFAEKVLLWSTIGTDGFVLDFTIFKSIQVNSTEHQVAVAGGVLMKELSTVLADAGECAAVGNANTIGVIPYCLGGGLGVATGLMGLACDNMLSAKVILADGRLVYVDNVHEPDLFWAIKGAGFYFGAVIEITLRTYPLTIFGTHDGRHWIGNFMYPLERAAEVFEVIETLVNTPKSRTACLLMIIAPPPYFQPTIAVVPHYFGDLTEGPKIFQCLTDLGPSFFSETTPLVPNLSDHLDFACGKGGFRRFTLAGLQELKAANILKLADLFQQLLDSCPDAASSGYFIEWHCLPPPDIPTNSAFSHHDVRIWIHCLSWCQRQENWDKVFGFEQRAIDTMRFGTKPETHVDLPHGTRTGPIEQRFKGQGRLTKLRALKKEFDPQGAFTKEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRX2</accession>
    <name>A0A8H6CRX2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007981</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156185.1">
        <property type="nucleotide sequence ID" value="XM_037298849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRX2"/>
    <dbReference type="GeneID" id="59336378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF282">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="32406" checksum="AC4852AD2955C354" modified="2022-01-19" version="1">MASTRKEAFSVNDMPSLAGFIAIVTGGSDGIGYVASYQLAIHGARVYIMSRSLQKAVKAISNMQKSTPGKELDLHFLQVDLQSLGSVNEAAQRFKETESRLDILVNNAGIMAVPYALTPDGYETQWQTNYLSPFFLIKSLLPVLCSTAAGTTSQGRVRIVNVSSDAAFVAITPDCDLENPNLDHLKGNLAIKAYAVHPGVIETNLQAADPTILGKIVKFSVKWGLVPGKLGREDGARTTLACATGEDKGIVEGSGAFFGPFGQRDKRGDALIRKWQPKNIEEKLWKASEKMLREKGFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSR3</accession>
    <name>A0A8H6CSR3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006964</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156495.1">
        <property type="nucleotide sequence ID" value="XM_037297860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSR3"/>
    <dbReference type="GeneID" id="59335364"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047389">
        <property type="term" value="F:glycerophosphocholine phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08606">
        <property type="entry name" value="GDPD_YPL110cp_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14484">
        <property type="entry name" value="SPX_GDE1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057506">
        <property type="entry name" value="C2_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051578">
        <property type="entry name" value="GDPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE PHOSPHODIESTERASE GPCPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25329">
        <property type="entry name" value="C2_GDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SPX" evidence="6">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="527"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="7">
        <location>
            <begin position="809"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="890"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="899"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="927"/>
            <end position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1167" mass="128597" checksum="D0C12DCA76CA6A82" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSR7</accession>
    <name>A0A8H6CSR7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006948</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156479.1">
        <property type="nucleotide sequence ID" value="XM_037297844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSR7"/>
    <dbReference type="GeneID" id="59335348"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051104">
        <property type="entry name" value="FAD_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720:SF3">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01460)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="21"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="306"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6228837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="430" mass="46845" checksum="3FB43A466A7FE846" modified="2022-01-19" version="1">MGSIPHATNGISSSEKAIRLAIIGGGIGGLCLAIGLLKQPHVDVQVYEAASTFSEIGAGVVIGNNAERALQLIGSAAWQAMEKHATRNLWSSHSDIFQVFRVGVGEKEGALISAPKNRSQSVHRARFLDELVKQVPAQRAHFNKRLQDLEETEDGVILHFNDGTTATADVAIGADGVHSRVRELLVGEEAAKPVFSGAVIYRGLLPMDRAIEVIGPEDAQNAVVLCGPGQVALSYPIDFGKNLNCGVFHFGLKKWPHEKWIVPADHEELTSSFKDWGEPARKIVKLLNLPTVSAWALFDSPPAPFFHRGRVAMMGDAAHATTPFQGQGAGQAIEDACVLSALFTKVVTPKDVPTALAAYDQIRRPRSQRVVQTSRETGHMLSMQLPGVGDDVEKMKQYLDTSMHWIWDRDLVAQNEDAVKLFEKLRCLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6R9</accession>
    <name>A0A8H6F6R9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147090.1">
        <property type="nucleotide sequence ID" value="XM_037297654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6R9"/>
    <dbReference type="GeneID" id="59335158"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051035">
        <property type="entry name" value="Mito_inheritance_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36091">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36091:SF2">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="62799" checksum="EDCF195BD647BE7A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7A2</accession>
    <name>A0A8H6F7A2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006354</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147377.1">
        <property type="nucleotide sequence ID" value="XM_037297252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7A2"/>
    <dbReference type="GeneID" id="59334755"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6217942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="98" mass="10706" checksum="EF1B1674FB0588B7" modified="2022-01-19" version="1">MAPAAASAGKKQKKKWSKGKVKDKANHAVILDKATSDKLYKDVQSYRLITVATLVDRLKINGSLARKALNDLEEKGQIKKVVGHSKLSIYTRAVTAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7P1</accession>
    <name>A0A8H6F7P1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147149.1">
        <property type="nucleotide sequence ID" value="XM_037297439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7P1"/>
    <dbReference type="GeneID" id="59334942"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="162" mass="17090" checksum="5529C2209F540317" modified="2022-01-19" version="1">MAEYSLGQASPTFTTSTTAAASATVGPMICNTYQTSYSHCWTAIDPNTVNSTADAMIGTQLPNGPMTPLTPNSTEVVTVKGLNYFMNIGWIPGCVAVNSQDPANPIASDQSIATRDILWQTYNDCTGNHGIGGYSDTLCLRIGFFPNKVAGVGGSPPTPRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F820</accession>
    <name>A0A8H6F820_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">DNA damage-binding protein CMR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005996</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">DNA-binding protein that binds to both single- and double-stranded DNA. Binds preferentially to UV-damaged DNA. May be involved in DNA-metabolic processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WD repeat DDB2/WDR76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148080.1">
        <property type="nucleotide sequence ID" value="XM_037296899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F820"/>
    <dbReference type="GeneID" id="59334401"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000001">
        <property type="term" value="P:regulation of DNA damage checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000562">
        <property type="entry name" value="DNA damage-binding protein CMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050853">
        <property type="entry name" value="WD_repeat_DNA-damage-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="335"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="525" mass="58072" checksum="44DDE5A2E449CD9E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8A7</accession>
    <name>A0A8H6F8A7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148397.1">
        <property type="nucleotide sequence ID" value="XM_037296415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8A7"/>
    <dbReference type="GeneID" id="59333912"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="348" mass="39008" checksum="66D6A72DC7FCADA5" modified="2022-01-19" version="1">MVMPVLHHRHSVSGSRQLDGAKQSIKSNNAMADHLWILDETYERPEKERHLSSTTIGRVPSKKDNPNHLRIDTYSKANLHDRYMSSEEEPSPSPDSDTESNEEELKHKVPEKVVEATTEPLTADEYEAEIAIAVPILAMGKPKLVDITNLAPMHKRKRAEKSVLSRSLVKNATLRMPTVTDENRPFVASEATKIVTPEAGPPTRKASLRSLAPDSWLPDDGVRIVQEEEEEEDEHYFPDLELRKAPTYNDYDPYSLSPPRLSPRNSYNSTSKKPGSVARARNTSNPPTTMNSGWKGLTRSMSLAKKQALHRADHQVSKKPKMIARAADEREEPLIIPAFPFGDGRGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8P7</accession>
    <name>A0A8H6F8P7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148740.1">
        <property type="nucleotide sequence ID" value="XM_037296042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8P7"/>
    <dbReference type="GeneID" id="59333536"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000046">
        <property type="entry name" value="dnaJ homolog subfamily C member 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051964">
        <property type="entry name" value="Chaperone_stress_response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="319"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="520"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="278"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="367"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="484"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="560" mass="62983" checksum="E77C2190C6C2D861" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8S4</accession>
    <name>A0A8H6F8S4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">20S-pre-rRNA D-site endonuclease NOB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005367</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the synthesis of 40S ribosome subunits. Has a role in processing 20S pre-rRNA into the mature 18S rRNA, where it is required for cleavage at the 3' end of the mature 18S rRNA (D-site). Accompanies the 20S pre-rRNA from the nucleus to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NOB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148259.1">
        <property type="nucleotide sequence ID" value="XM_037296277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8S4"/>
    <dbReference type="GeneID" id="59333773"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000020">
        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.10">
        <property type="entry name" value="Nin one binding (NOB1), Zn-ribbon-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017117">
        <property type="entry name" value="Nob1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036283">
        <property type="entry name" value="NOB1_Zf-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014881">
        <property type="entry name" value="NOB1_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08772">
        <property type="entry name" value="Zn_ribbon_NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037125">
        <property type="entry name" value="D-site_20S_pre-rRNA_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144206">
        <property type="entry name" value="NOB1 zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ribonuclease PIN" evidence="11">
        <location>
            <begin position="10"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Nin one binding (NOB1) Zn-ribbon-like" evidence="10">
        <location>
            <begin position="290"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="416"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037125-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6218824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="441" mass="47922" checksum="88D6778D2BB44D11" modified="2022-01-19" version="1">MTLRKPVHTIVLDAGPLLKNEPTVSTLISQCENLFTIPSVIAEIRDENARSRVETTLLPFLTIRSPSERSLRFVTDFSRKTGDLAVLSKTDLLCLALTHELECERNGGDWRIRNAPGQKGVNGRKEQAASGDASEATNQSSTIETQPTGDTDRSQEPDTVAPFEAATSDQGCVPLGARDSTTADEANGVSESLGSLKFTDEDVQQDSVAASGPESSEIEQIEASSDSSDSEGWITPKNVKKHQAKDVNGSALPIPENQPIQVALLSGDFAMQNVCLQIGLNLLSPSLQRVRNIRTYILRCHACFAKEKDMSKQFCSKCGKPTLTRVSCSTNSKGEFKIHLKKNMQWNHRGDRFSIPKPVPGAANGKVGQGKGGGKGGWGQELILAEDQKEYLRAMDGRNKKKEMDLMDEDYLPSILTGDRGKAGGRPKIGAGRNVNSKKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8V0</accession>
    <name>A0A8H6F8V0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aromatic prenyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005442</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tryptophan dimethylallyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148334.1">
        <property type="nucleotide sequence ID" value="XM_037296352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8V0"/>
    <dbReference type="GeneID" id="59333848"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009820">
        <property type="term" value="P:alkaloid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13929">
        <property type="entry name" value="PT-DMATS_CymD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033964">
        <property type="entry name" value="ABBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017795">
        <property type="entry name" value="ABBA_NscD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03429">
        <property type="entry name" value="arom_pren_DMATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40627">
        <property type="entry name" value="INDOLE PRENYLTRANSFERASE TDIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40627:SF4">
        <property type="entry name" value="PRENYLTRANSFERASE ASQH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11991">
        <property type="entry name" value="Trp_DMAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00036">
        <property type="entry name" value="Aromatic_Prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01162">
        <property type="entry name" value="I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="48226" checksum="2A7C36D3AAEC8E60" modified="2022-01-19" version="1">MNGEGPSYWWRTSGSDLSRMLHEANYPEEAQRQFLDYYRDTLCPLLGNKPESNSLPAAVGWDGNPFEYSFEFKGSTKTPGVRFVLDLSELRPPNKDHPLSIANSEKVLETLAKQSPMFDDKWHRALARWFVYSNVSPSKQQALVAQVGYQTPTILGFDINARILELAPGMLPVMAKSYFPPCFVAADKGFGRFQALSLGIRQMPDIGYYPNILLALKTIEDYVEDHPQYENCGRGMATDFVKAGKARLKVYMRYWGNSFDEMWDYYTLGGRIPDLEDDKEKLRDLIDLASGCDYPADKIKEETPAEQRRRALFGQKPTSMYFSLSPDRPYPVPKLYFYPAFRAPNDQAIAQGLDAWLTKYGWHDGGRSLEERLENVFTYRKLDEKPGIFTFIGIGRKEDSKQDGLSLQVYVTPELYETPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F955</accession>
    <name>A0A8H6F955_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anhydro-N-acetylmuramic acid kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149220.1">
        <property type="nucleotide sequence ID" value="XM_037294532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F955"/>
    <dbReference type="GeneID" id="59332021"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006040">
        <property type="term" value="P:amino sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005338">
        <property type="entry name" value="Anhydro_N_Ac-Mur_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30605">
        <property type="entry name" value="ANHYDRO-N-ACETYLMURAMIC ACID KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30605:SF0">
        <property type="entry name" value="ANHYDRO-N-ACETYLMURAMIC ACID KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03702">
        <property type="entry name" value="AnmK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16087" checksum="C471A1A3D430A24E" modified="2022-01-19" version="1">MGAKAKVGQAIVDEMLQQPYFVHDVPKTTGRETFGGRMEGGKPRGLRITAYSLLEHYRRYGPKQLGEICMGGGGSYNPNIVNYLREQTPTTSITTINEIGIPIGAKEALNFAFLGFEGFVGRPIIIPKNARKGAIGQTEPGDNYFHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAL6</accession>
    <name>A0A8H6FAL6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150283.1">
        <property type="nucleotide sequence ID" value="XM_037293454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAL6"/>
    <dbReference type="GeneID" id="59330941"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="66230" checksum="8F0B856184DD0D06" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAP4</accession>
    <name>A0A8H6FAP4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002306</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150885.1">
        <property type="nucleotide sequence ID" value="XM_037293232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAP4"/>
    <dbReference type="GeneID" id="59330719"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF74">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="162"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="28945" checksum="74867E837B863752" modified="2022-01-19" version="1">MPSLREIIIRAASKGHLEVLKRLLLPNPSYSLQEADTTLEKLVGIAARHCHSKVLEFCISLGTNVNDDAVRIGVLESSRLNVYQKVIAAGFDLNYDHDGTIGGPLIWATLTNHIPLATYLLDHGADVNRDLQNRVYRPLAKAAEKNSVAMIELFIRYGAQMDRSGALIVAAEHGNLEAVRCLVSHGADINLIRKSDTDLYMKTGEEESALHKAVRGGHEDVVAFLVESGAHLGLRDHQGDDALMMAVKMNSAELFQIIYDARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB10</accession>
    <name>A0A8H6FB10_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002352</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150931.1">
        <property type="nucleotide sequence ID" value="XM_037293278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB10"/>
    <dbReference type="GeneID" id="59330765"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000125">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF22">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G11480)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="88"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="118"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="136"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="238"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="494" mass="56103" checksum="F6846A1CCF61B3C5" modified="2022-01-19" version="1">MVDKATTKQSPTVKGGASAADMAARPFQCEWHEGCPKSFNRKSDLQRHYRIHTNERPYACDYKPCSKTFIQRSALTVHRRTHTGDKPHKCPFTGCGKCFSDSSSLARHRRIHTNDKPYICRNQRCKKAFCRKTTMTKHIRKEHPAEPIHEDQDAEYSDEDASEEEELEEDTEEGKEDPQLPYQEPAELDQRNQSVGAPSHYNRSLWRLPGETVQRPSPLRLQRPIPRSEAPIIHEIKLERSCSSTPQRSLTDPYPTGPMHSSEYSPVRADTMPDGLPQAPASAGVPPQYQLGNNSNMGIWSPQSLQDSPTSLTHSSPSSASTQSHSLFASQSYQQFQQVSIPEPMSYPDHQETVQNLEQLKLGQSQLHQYGALQQNVSQQDAYTGMSREASQNHAYNGTQAQSVAQHYQSDMPPTPAPTQSMPQYTAPIQEVPYQAPQFLPPDNYPIGNQYFSPHINGLYQYNDGMDWWKEEVKPEDYWTLMPSQRMQGNDWPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB87</accession>
    <name>A0A8H6FB87_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001704</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151171.1">
        <property type="nucleotide sequence ID" value="XM_037292632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB87"/>
    <dbReference type="GeneID" id="59330118"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061574">
        <property type="term" value="C:ASAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481:SF0">
        <property type="entry name" value="LD23870P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481">
        <property type="entry name" value="RIBONUCLEIC ACID BINDING PROTEIN S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="108"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="358" mass="39236" checksum="6B89F33F97D170DB" modified="2022-01-19" version="1">MPSVSPERGRTRSRHQTSAFAKSQTPSRTRSAPRRTISPRSRSRSRTPSRTSSAQGDKRNGVRNGKERSRSPTRSASRSRSEGRAGRRYRDPSYTRSPSHGSLLPKSSKIVVEKLTKNVNEGHLREIFGAYGSIRELDLPMNRQFMANRGTAYIVYTDTPHAEAAIAHMHEAQLDGAIINVSIVLPRQVLLLMIASRHAHLRLADIEEDPPRLTMDPLAVEDIALRNPQEEAHHKGGMEAVQPREIAIQTHTGRAHSLVPDHREHAHGPILPDRGPGLPHVEAETTDVETAHRHRGEAGEEGVLATPAFPAIAIGAVAGVEVDMDAEGDSVPAWDPLFRSSMVDIGTLPCIRDVILGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBG4</accession>
    <name>A0A8H6FBG4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Fatty acid synthase subunit beta</fullName>
            <ecNumber evidence="5">1.3.1.9</ecNumber>
            <ecNumber evidence="7">2.3.1.38</ecNumber>
            <ecNumber evidence="8">2.3.1.39</ecNumber>
            <ecNumber evidence="4">2.3.1.86</ecNumber>
            <ecNumber evidence="3">3.1.2.14</ecNumber>
            <ecNumber evidence="6">4.2.1.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="32" type="ORF">HO133_001642</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="32">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="26">Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The beta subunit contains domains for: [acyl-carrier-protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(9Z)-octadecenoyl-[ACP] + H2O = (9Z)-octadecenoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15057"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9924"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78783"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41788"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="EC" id="2.3.1.38"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">[Alpha(6)beta(6)] hexamers of two multifunctional subunits (alpha and beta).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 28">Belongs to the fungal fatty acid synthetase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="32">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.9" evidence="5"/>
    <dbReference type="EC" id="2.3.1.38" evidence="7"/>
    <dbReference type="EC" id="2.3.1.39" evidence="8"/>
    <dbReference type="EC" id="2.3.1.86" evidence="4"/>
    <dbReference type="EC" id="3.1.2.14" evidence="3"/>
    <dbReference type="EC" id="4.2.1.59" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151109.1">
        <property type="nucleotide sequence ID" value="XM_037292570.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330056"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004313">
        <property type="term" value="F:[acyl-carrier-protein] S-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03447">
        <property type="entry name" value="FAS_MaoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.120:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000017">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000078">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.100:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000015">
        <property type="entry name" value="Fatty acid synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3330:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit beta dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000003">
        <property type="entry name" value="Fatty acid synthase subunit beta dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039569">
        <property type="entry name" value="FAS1-like_DH_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016452">
        <property type="entry name" value="Fas1/AflB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013565">
        <property type="entry name" value="Fas1/AflB-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041099">
        <property type="entry name" value="FAS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040883">
        <property type="entry name" value="FAS_meander"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003965">
        <property type="entry name" value="Fatty_acid_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08354">
        <property type="entry name" value="Fas1-AflB-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13452">
        <property type="entry name" value="FAS1_DH_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22235">
        <property type="entry name" value="FAS1_thioest_ins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17951">
        <property type="entry name" value="FAS_meander"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17828">
        <property type="entry name" value="FAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005562">
        <property type="entry name" value="FAS_yeast_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01483">
        <property type="entry name" value="FASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11 28" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18" id="KW-0456">Lyase</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15 28" id="KW-0520">NAD</keyword>
    <keyword evidence="13 28" id="KW-0521">NADP</keyword>
    <keyword evidence="14 28" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 28" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="31">
        <location>
            <begin position="1687"/>
            <end position="1987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="2073"/>
            <end position="2094"/>
        </location>
    </feature>
    <feature type="active site" description="For acetyltransferase activity" evidence="29">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <feature type="active site" description="For malonyltransferase activity" evidence="29">
        <location>
            <position position="1831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00033756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00058855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00068309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRNR" id="PIRNR005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR005562-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="EMBL" id="KAF6221674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2094" mass="232628" checksum="67B315E39098FA57" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBJ8</accession>
    <name>A0A8H6FBJ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151258.1">
        <property type="nucleotide sequence ID" value="XM_037292719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBJ8"/>
    <dbReference type="GeneID" id="59330205"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="169" mass="17994" checksum="5F5FA09AA9844EAE" modified="2022-01-19" version="1">MRQQNQPDRNPRPQNRPLQVIESRSVQPREPRAGEVHHPPQERISGTPRYGSFGGVESEAQTAVGEREDLCGGYSGPVAHEGVVDGGVHVRDGLGGAGEGGKGFPVVEDDVPATDDTVPGGEIVAGVSSYLSVRGHMRSALSIAAEANRADGYDFFFRESMLQHIHGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBU4</accession>
    <name>A0A8H6FBU4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001180</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151529.1">
        <property type="nucleotide sequence ID" value="XM_037292110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBU4"/>
    <dbReference type="GeneID" id="59329596"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20568">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20512">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="441"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="7">
        <location>
            <begin position="534"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="538"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="195"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="681" mass="76157" checksum="05D38DCD67F90930" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCI6</accession>
    <name>A0A8H6FCI6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pantothenate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000939</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152234.1">
        <property type="nucleotide sequence ID" value="XM_037291872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCI6"/>
    <dbReference type="GeneID" id="59329357"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24123">
        <property type="entry name" value="ASKHA_NBD_PanK-II_Pank4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000115">
        <property type="entry name" value="Pantothenate kinase PanK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.510:FF:000005">
        <property type="entry name" value="Probable pantothenate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00555">
        <property type="entry name" value="panK_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF20">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="46643" checksum="6E0204C31F037308" modified="2022-01-19" version="1">MSDSRRPTLQERRNSVLDETILSPGSVKINVQGAFIADEVASITGNQPDDGAQHDTRCIRLPNHTGVVSHVALDIGGSLAKLVYFSREPDFKELGGRLNFLKFETDRIDQCFKVVQELKAKQQPLNGSQPGELCIVATGGGAYKFYDEMKNVLGVEVLREDEMECLIMGLDFFITEIPREVFTYADLDPMHFEEPRANIYPYLLVNIGSGVSMVKVSGPKQFERIGGTSLGGGTLWGLLSLLTGARTFDGMLAMADRGDNTTVDMLVGDIYGTDYSKIGLKTSTIASSFGKVFKMKRQGEDDAEDGNGLSNGDPQPASGSSDPDRAFAPEDISRSLLYAVSNNIGQIAYLQSEKHKLEHIYFGGSFIRGHRQTINTLSYAIRFWSNGEKEAYFLRHEGYLGSVGAFLKRQPKIWGRRNSFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCJ4</accession>
    <name>A0A8H6FCJ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000182</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152557.1">
        <property type="nucleotide sequence ID" value="XM_037291122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCJ4"/>
    <dbReference type="GeneID" id="59328601"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033370">
        <property type="entry name" value="COG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31658">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31658:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="675"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="694"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="717"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="827" mass="94418" checksum="1AFEE6EA00E45291" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCJ9</accession>
    <name>A0A8H6FCJ9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152572.1">
        <property type="nucleotide sequence ID" value="XM_037291137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCJ9"/>
    <dbReference type="GeneID" id="59328616"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="62" mass="7159" checksum="ED03784F2DA828FF" modified="2022-01-19" version="1">MNNIQQKLPSFRSNDVMYLIIDQVFHDEAHYPQRTYARGPTWATRAPTKDIDAALIRAEAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCN5</accession>
    <name>A0A8H6FCN5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosducin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosducin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152355.1">
        <property type="nucleotide sequence ID" value="XM_037291993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCN5"/>
    <dbReference type="GeneID" id="59329479"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02988">
        <property type="entry name" value="Phd_like_VIAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051498">
        <property type="entry name" value="Phosducin-like_chap/apop_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024253">
        <property type="entry name" value="Phosducin_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809:SF3">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02114">
        <property type="entry name" value="Phosducin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosducin" evidence="3">
        <location>
            <begin position="67"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="28119" checksum="B7B2113E9D2D2A8F" modified="2022-01-19" version="1">MDMPMNVPVDDPNADTEWNDILRKHGVIPEKPPSPTPLIQEALAEARKQAYGNRLEEKDLDELNDLEDEEDEAFLAKYRNQRMAELSTIATASVFNQVYPLQKPDYPKDVTEASNKAFVLVHLTSSLGTNIESRLLTELWREMARKFGDIKFCEIRADMCIEGYPERNTPTILVYRNGDIKKQVVTLNELGGDHTTIKDLQNLLVGVGALKATDHRVVNSTRGDGEVPYSSIRTGNLNAATAEDDDDDWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCW0</accession>
    <name>A0A8H6FCW0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152902.1">
        <property type="nucleotide sequence ID" value="XM_037291467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCW0"/>
    <dbReference type="GeneID" id="59328947"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="192" mass="20885" checksum="99E74AC7BE2209A3" modified="2022-01-19" version="1">MPNEELQVGNQEKRHVGPFGKMMEIGKRDLIGHGQLRMEKPLSWKLDVRMSIYSSLEVSASLGTPTAGNESLKAFLSAVAADPSILAASSGLDFLSHEIGACLCSSVQTSVEELSVTRSLVAMDIDSLVAIEVRNWWRQALAVLRLEITEIIGSENVEALGKIALERLKAKHSAQAKQDEEIADTCLLMKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD65</accession>
    <name>A0A8H6FD65_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">4-hydroxybenzoate polyprenyltransferase, mitochondrial</fullName>
            <shortName evidence="11">4-HB polyprenyltransferase</shortName>
            <ecNumber evidence="11">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Para-hydroxybenzoate--polyprenyltransferase</fullName>
            <shortName evidence="11">PHB:PPT</shortName>
            <shortName evidence="11">PHB:polyprenyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010726</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>an all-trans-polyprenyl diphosphate + 4-hydroxybenzoate = a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:44504"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:9564"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Mitochondrion inner membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
            <orientation evidence="2 11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153212.1">
        <property type="nucleotide sequence ID" value="XM_037301578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD65"/>
    <dbReference type="GeneID" id="59339116"/>
    <dbReference type="UniPathway" id="UPA00213"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate polyprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1780:FF:000001">
        <property type="entry name" value="4-hydroxybenzoate octaprenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000003">
        <property type="entry name" value="4-hydroxybenzoate polyprenyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01474">
        <property type="entry name" value="ubiA_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="340" mass="36414" checksum="0EDCF7E8B44B77CB" modified="2022-01-19" version="1">MTHSEATPAVPSSKTVLPIYEAPQTGFISHLPGTWIPYAELMRLDKPAGTYYLFFPCLFSTLLAASYTASGLLPVSTTTLCFFSGALIMRGAGCTINDLWDRNLDPHVARTRLRPIARGAVTPFKALIFTGFQLLAGLGVLLQFPASCLYYGIPSLLLVATYPLAKRVTHYPQFVLGLTFSWGAFMGFPALGVDPLADSAVLKAMAALYASCVSWTVSYDMIYAHMDIKDDAKVGIKSIALRHEKETKAVLSGLAVVQVGLLAAAGTAAGVGPVFFIGSCGSAAATLGFMIWKVRLNEISNCWWWFTNGAWLTGGGIAGGMLAEYVLNSQQKIEATESDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDA2</accession>
    <name>A0A8H6FDA2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_010694</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin Nup133 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153180.1">
        <property type="nucleotide sequence ID" value="XM_037301546.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59339084"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007187">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014908">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037624">
        <property type="entry name" value="Nup133-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13405">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13405:SF11">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03177">
        <property type="entry name" value="Nucleoporin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08801">
        <property type="entry name" value="Nucleoporin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like N-terminal" evidence="10">
        <location>
            <begin position="115"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like C-terminal" evidence="9">
        <location>
            <begin position="662"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1340"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1340"/>
            <end position="1352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6224120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1365" mass="151958" checksum="CF057AEF94AA50AB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDW6</accession>
    <name>A0A8H6FDW6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mob1/phocein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009974</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153647.1">
        <property type="nucleotide sequence ID" value="XM_037300830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDW6"/>
    <dbReference type="GeneID" id="59338366"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="46192" checksum="18113FA730066029" modified="2022-01-19" version="1">MVTSPSPSSSPRAYSPPPAPEVQIAGPQSPAMIGEPGPTIPEPEDTAKLDQGATRRIRPGTKAADMASGPPLVPLNQLDSPFQLQEHLKALHYEHTRSKTDPTATVSLTHETALLIATPPPNIDRSLWLYELTRFLTQHANDLLVALLTKIDPACSAASCPEMRASEWQYLCAVHDTPKACCAVDYCIHTLDWAGDILTSVKLFPSRMTLGPPDSPGHQTAVRNITNIMRRVYRIFAHAWFQHREGVFWPVEASGGIYTLFKTVCDVYGLIQEDNYTIPPEAEGLPSKEEIEHDRQEKAGMKKTGDEEGEEDATALQIAGATTRRHKNTPSVGSAVGTIAEGEEDESESPVKEKAGPLAQISNAAEKKDGVPGSAGSGTAVGDAMKEENIAGMVKEGEDSGDTPEDDGDDTASVVTAIHDDTEKKAEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE34</accession>
    <name>A0A8H6FE34_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-ornithine N(5)-monooxygenase [NAD(P)H]</fullName>
            <ecNumber evidence="4">1.14.13.196</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_011071</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-ornithine + NADH + O2 = N(5)-hydroxy-L-ornithine + NAD(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:41512"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78275"/>
            <dbReference type="EC" id="1.14.13.196"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-ornithine + NADPH + O2 = N(5)-hydroxy-L-ornithine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:41508"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78275"/>
            <dbReference type="EC" id="1.14.13.196"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Siderophore biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.196" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153554.1">
        <property type="nucleotide sequence ID" value="XM_037301920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE34"/>
    <dbReference type="GeneID" id="59339461"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025700">
        <property type="entry name" value="Lys/Orn_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42802:SF1">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42802">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13434">
        <property type="entry name" value="Lys_Orn_oxgnase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6224494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="459" mass="51654" checksum="A27BE47E7EED18DE" modified="2022-01-19" version="1">MLSEPAHHTSQTSAALQYQYDLVCIGFGPASLAIAIALHDREIKARVLYIERQRTFVWHAGMLLPNARMQISFLKDLATLRNPRSKFTFVNYLKSKNRLVAFTNLSTFLPLREEYNDYMSWCASHFEQDVQYGHETICVSPAENRMGPIQAWRVLSKNVETNETNTVTARSVVIAIGGKPRIPPALSTGGLDCRIIHSSLYSKAAPSILTDASKAHNVAVIGGGQSAAEIFNDLQSRYPNSYITLYTGASALKPSDDSPFVNEIFDPERVDMFYDLPTESRLKTIWSDKATNYGVVRPELLDRMYENMYHQRLHEPDQSKWQYKIVAWREVVGFETCSDRRIRLKLKNTSKGEITMSDSSFDLAVLGTGYERNGHETLLEPARHLFRESRYAVERNYKVKFRKDAVASDCGIWLQGCCEDSHGLSDTLLSILAVRGGEMVDAIFPMQSPGPGKSGCAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF36</accession>
    <name>A0A8H6FF36_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154495.1">
        <property type="nucleotide sequence ID" value="XM_037300647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF36"/>
    <dbReference type="GeneID" id="59338183"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="464" mass="50990" checksum="BF83396D5C86A874" modified="2022-01-19" version="1">MATTPPSTLAVYTPPTPLHGAKYDEHCPKPTRKATRNSSSRTQRAIETPPPNPVNSQHISASLDPKKPNTLRPAPHTYSPPSSTHTSPRKKSLRGPFSYHNKDASAMDGAGPSSGAPSLDLFHNPDKNPTLQQPSLHPAANMLPTPAKTPRKKPVQPAAVQAAARVLFPSRPDTVEDAMPNPRKRRNKRHVGFSLYSSMEDDGDAGSENQIEIYTDSKDKVPELDPSEDNPFYDQHARTVASVEPRKTKASKKRKAEHSIDTSTEIEEAFNREEGMVYVFRGKKMYRKFPHDGLEYNDTNNRGDSERESSTIRPLTRSSIKPRLLFPTTQQIHERTVSTIDDEEAPTDIEDPQDHELTDPEDEKPITTPIKASVFSPATPPTTGHATRAVTKKAALDSPSGRPEPVQAVPYERRGKKVSPFDAWARTKAGMSGSEGKGKKREGEAMEKNEGVAGSKKVRGSGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF57</accession>
    <name>A0A8H6FF57_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009848</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154555.1">
        <property type="nucleotide sequence ID" value="XM_037300707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF57"/>
    <dbReference type="GeneID" id="59338243"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025340">
        <property type="entry name" value="DUF4246"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049192">
        <property type="entry name" value="DUF4246_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049207">
        <property type="entry name" value="DUF4246_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33119:SF1">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33119">
        <property type="entry name" value="IFI3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14033">
        <property type="entry name" value="DUF4246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21666">
        <property type="entry name" value="DUF4246_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4246" evidence="2">
        <location>
            <begin position="29"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4246" evidence="1">
        <location>
            <begin position="77"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="555" mass="64760" checksum="63CE004DBC864ECF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGJ2</accession>
    <name>A0A8H6FGJ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">U6 snRNA-associated Sm-like protein LSm6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_008759</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner, facilitating the efficient association of RNA processing factors with their substrates. Component of the cytoplasmic LSM1-LSM7 complex, which is thought to be involved in mRNA degradation by activating the decapping step in the 5'-to-3' mRNA decay pathway. Component of the nuclear LSM2-LSM8 complex, which is involved in splicing of nuclear mRNAs. LSM2-LSM8 associates with multiple snRNP complexes containing the U6 snRNA (U4/U6 di-snRNP, spliceosomal U4/U6.U5 tri-snRNP, and free U6 snRNP). It binds directly to the 3'-terminal U-tract of U6 snRNA and plays a role in the biogenesis and stability of the U6 snRNP and U4/U6 snRNP complexes. LSM2-LSM8 probably also is involved degradation of nuclear pre-mRNA by targeting them for decapping, and in processing of pre-tRNAs, pre-rRNAs and U3 snoRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155624.1">
        <property type="nucleotide sequence ID" value="XM_037299623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGJ2"/>
    <dbReference type="GeneID" id="59337154"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01726">
        <property type="entry name" value="LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000037">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF1">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11 15" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="9 15" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Sm" evidence="16">
        <location>
            <begin position="11"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6227316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="80" mass="8746" checksum="1F7041504F8D2A42" modified="2022-01-19" version="1">MENGANSGSSDPSSFLTEIIGAPVKVKLNSSVEYRGYLISVDGYMNISLEKTEEYVDGKLRRSYGEAFVRGNNVLYISAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGL5</accession>
    <name>A0A8H6FGL5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155412.1">
        <property type="nucleotide sequence ID" value="XM_037299411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGL5"/>
    <dbReference type="GeneID" id="59336941"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027796">
        <property type="entry name" value="OTT_1508_deam-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14441">
        <property type="entry name" value="OTT_1508_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="769" mass="85922" checksum="783953FAF9C3DEAD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGM3</accession>
    <name>A0A8H6FGM3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155442.1">
        <property type="nucleotide sequence ID" value="XM_037299441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGM3"/>
    <dbReference type="GeneID" id="59336971"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="108" mass="11691" checksum="DB7240DE30370C9C" modified="2022-01-19" version="1">MRACCASKPADAMENDPHNNPSSGANASMTRQISGYAKHLYNAGEHLIEVDALCQPSPAGVKFRAMVKITRGKEVERHDLQNEAYGTLEASLSALLQTTMTKIEDQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH94</accession>
    <name>A0A8H6FH94_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="5">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007809</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156015.1">
        <property type="nucleotide sequence ID" value="XM_037298679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH94"/>
    <dbReference type="GeneID" id="59336206"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000009">
        <property type="entry name" value="Peptide-methionine (R)-S-oxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081:SF8">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="6">
        <location>
            <begin position="28"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="160" mass="17769" checksum="232C566D6C91AE41" modified="2022-01-19" version="1">MPSVPFLGSLFGGRQSRDMTDYPVKKGEDEWQAVLSPEQFRVIRQKGTEAPFTGEYDRHMPSEGTYECAACHTPLYRATHKFKSGCGWPAYFDSIPGAVTRHTDSTFGMARTEILCSKCGGHLGHVFKGEGYPTPTDERHCVNSISLKFAKEDREGKSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHH1</accession>
    <name>A0A8H6FHH1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RED-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008036</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156240.1">
        <property type="nucleotide sequence ID" value="XM_037298904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHH1"/>
    <dbReference type="GeneID" id="59336433"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039896">
        <property type="entry name" value="Red-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012916">
        <property type="entry name" value="RED_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12765">
        <property type="entry name" value="RED PROTEIN IK FACTOR CYTOKINE IK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07808">
        <property type="entry name" value="RED_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RED-like N-terminal" evidence="4">
        <location>
            <begin position="78"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="68"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="324"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="352"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="451"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="543" mass="59426" checksum="C5683E8DB9524647" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHH7</accession>
    <name>A0A8H6FHH7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidase FUB9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Fusaric acid biosynthesis protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007717</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
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        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="8">
        <location>
            <begin position="13"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
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        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="246"/>
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        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="301"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="324"/>
            <end position="325"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00083297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6227989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="382" mass="41520" checksum="84325FBB386A2CBE" modified="2022-01-19" version="1">MAGGAPVPVPTSDPDEKILSIADLEKAASTKLGKTAREFYNSGSTDQVTIHENSTAFSKYRLRPRVLVDVSGVDTSTTAFGRSISFPLSVSPAGLQAMAHPDGELGTSKACARKGVNMAISSFSNHSIEDVRSAAAEIGPIDHAVQLYTMRDRELEERIIRKAEAAGCKAIFLTADSPVLGVRYNEWKNDFRTPEGLGFPVIEWTSEEVRSRTHDSGFVAFNDDSHSWARDIPWLKARTKMQIWIKGVLTAEDTELAVEHGCDGIIVSNHGGRQLDGVMPTLDALPECVEAAKGRIAVHVDGGFRSGTDIFKALALGANCCWVGRPAIWGLAYDGEAGVDLMLETMHNEFRRCMQLTGCRTVKDINKTCLARVNADGILKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI18</accession>
    <name>A0A8H6FI18_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156486.1">
        <property type="nucleotide sequence ID" value="XM_037297851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI18"/>
    <dbReference type="GeneID" id="59335355"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034508548" evidence="2">
        <location>
            <begin position="35"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="172" mass="18504" checksum="7105368E58FB5F1E" modified="2022-01-19" version="1">MKLSPSSSPLLSFCYLHTLTLAALSVVSPHPANAMATLNSTTPPPNPARPPLDPTQLRVHGTTTSLVFGKYTAPVSPFDLVGIVEKAQFEIVQSVVKARGDAPIAQPTFEWRHGRLYVRVEHELALTWLVMAEALHGLRDFGDEYGFWGLSFTVLDDTAGVVGSGSVGVGYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIL0</accession>
    <name>A0A8H6FIL0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007322</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156848.1">
        <property type="nucleotide sequence ID" value="XM_037298213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIL0"/>
    <dbReference type="GeneID" id="59335721"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47431:SF5">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47431">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="266"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="711" mass="78079" checksum="F508476ABA56E096" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIS5</accession>
    <name>A0A8H6FIS5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_007395</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing and cell cycle control.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CEF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156921.1">
        <property type="nucleotide sequence ID" value="XM_037298286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIS5"/>
    <dbReference type="GeneID" id="59335794"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11659">
        <property type="entry name" value="SANT_CDC5_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000021">
        <property type="entry name" value="CDC5 cell division cycle 5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047242">
        <property type="entry name" value="CDC5L/Cef1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021786">
        <property type="entry name" value="Cdc5p/Cef1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047240">
        <property type="entry name" value="SANT_CDC5L_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885:SF1">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11831">
        <property type="entry name" value="Myb_Cef"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="53"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="11">
        <location>
            <begin position="57"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="111"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="222"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="394"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6229279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="793" mass="88594" checksum="C5BA713F6D3CA56D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ09</accession>
    <name>A0A8H6FJ09_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157043.1">
        <property type="nucleotide sequence ID" value="XM_037298408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ09"/>
    <dbReference type="GeneID" id="59335916"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="36063" checksum="DB43B5C896227953" modified="2022-01-19" version="1">MAHLITGNKFEPNKDIPDLSGKVYIVTGGTAGIGFGITAHLLQHNASKILLLSQKEEHADEAIEELKKYGDASKVHWVQCDLKDLKKTDEVAKKLTSEKQIDALICNAGQGVGKYNETVDGIDSHFQVNHLSQMLLALTLLPNLQSTPNSRLVLQSSDLHRSPLSSTKFATLDEINTDLGPNSLYSRTKLAQILFVRALVRRMLNNEPGFQSPKYNGPWINATHPGGVATDQLGQAVDAYGTMGKIGVKAIKPIMKDPVDEGCRPALFAATSEDITKEGIQGQYIVPDRKVTEPSKQARDEELGENLWRLSEQILQEKLGSIPYKQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKK4</accession>
    <name>A0A8H6FKK4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004586</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157503.1">
        <property type="nucleotide sequence ID" value="XM_037295502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKK4"/>
    <dbReference type="GeneID" id="59332993"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF792">
        <property type="entry name" value="GM14286P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="280"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="378" mass="41609" checksum="9BAD85AD9D6F25C7" modified="2022-01-19" version="1">MQLPSNSISEDDYLSATGREEGEIEWMLFGIYDGHAGWETAAALRGYLFTYIAYELEAISSTGAPSDAAPQDVDRAIEKAFLKLDKEIMDQGATAITGPSFLNEAMSRLGPAYSGSCALVSYYHTESQILKVACTGDSRAVLGRRNAAGEWEAIALSSDQTGHNKDEVARLHKEHPDEPKLVKDGRVLGLMVSRAFGDGRWKWSRQVQEEAQRRFFGPPLREPLISPPYLTALPVVTTTKIEPENGDFLIMASDGLWDHLTSEQAVDLVSQWLKTHDVTKDVSPPDLAPTPNAIPAREISSRRNPNPNMAYTDIRTADAKHFVMRDENAATHLTRNALGGGNEDMLAGLLTVSPPYSRNVRDDITVNVIFFGKDDTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKL9</accession>
    <name>A0A8H6FKL9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004597</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP domain-containing ribonucleoprotein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157514.1">
        <property type="nucleotide sequence ID" value="XM_037295513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKL9"/>
    <dbReference type="GeneID" id="59333004"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052240">
        <property type="entry name" value="SAP_domain_ribonucleoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040746">
        <property type="entry name" value="THO1_MOS11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551:SF1">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18592">
        <property type="entry name" value="Tho1_MOS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="4">
        <location>
            <begin position="5"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="176"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="209"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6230257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="270" mass="28583" checksum="711052811EE77C30" modified="2022-01-19" version="1">MSTDYAKKKNAELEDLLKTRSLPHTGKKADLIARLQQYDTEQAATSNIATTAGAEDEIDWEDDVAAAEPTGAAAIAAGGLGQPTNPTAVPNQVVDTDPSKTNDLTVDPPAPTTEDTKAAASVEDVKATEADKKRSLPSTGIKATTLDDELAKRQKRAARFGMQESDEDALKAIERAKKFGTGDVGDRVAVKGLDEALPERAARGQKRGRGGDDGGRRDGREKRSRVRGGGRNGRERSGTPKDGVKKAGSGLSDKDRSAAEARKKRFAAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKR9</accession>
    <name>A0A8H6FKR9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157592.1">
        <property type="nucleotide sequence ID" value="XM_037295591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKR9"/>
    <dbReference type="GeneID" id="59333082"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="151" mass="17520" checksum="C65DC84032518111" modified="2022-01-19" version="1">MLDRFLHELWLIQLPAQGQRQALERHEGDREEEETGAEKAEEAEMIRIQRGRKGISLSLRWEREGMLPVRLLKLFNGGLSTALQDRRRIQYMFSSAPLDRMPSFSEPFRTAVQNSHLSNDPEQDISTNILDAYDDGSRLRGMFGMQQVMIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKS7</accession>
    <name>A0A8H6FKS7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157607.1">
        <property type="nucleotide sequence ID" value="XM_037295606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKS7"/>
    <dbReference type="GeneID" id="59333097"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="152" mass="16101" checksum="59774C3E9E477D16" modified="2022-01-19" version="1">MAPMPGFTAEQTASLQVMMTAAISQAMTAFETRLTDLEARTKPWQQSNADKIVEKTAEKPTEKTAEKKSIASSPIQAGTLSTIPHQADMHYCGESAIRLSNAPLLASLLASLLASLLASLLASLLASFLASQLASPLGYIRYAKDMEATGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKT2</accession>
    <name>A0A8H6FKT2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial</fullName>
            <ecNumber evidence="15">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_004685</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Complex I is composed of about 40 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Mitochondrion inner membrane</location>
            <topology evidence="15">Peripheral membrane protein</topology>
            <orientation evidence="15">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157602.1">
        <property type="nucleotide sequence ID" value="XM_037295601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKT2"/>
    <dbReference type="GeneID" id="59333092"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.230:FF:000001">
        <property type="entry name" value="Mitochondrial NADH dehydrogenase flavoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.600:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11540:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050837">
        <property type="entry name" value="ComplexI_51kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011537">
        <property type="entry name" value="NADH-UbQ_OxRdtase_suF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054765">
        <property type="entry name" value="SLBB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01959">
        <property type="entry name" value="nuoF_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22461">
        <property type="entry name" value="SLBB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="Nqo1 FMN-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142984">
        <property type="entry name" value="Nqo1 middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="Nqo1C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00644">
        <property type="entry name" value="COMPLEX1_51K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 15" id="KW-0288">FMN</keyword>
    <keyword evidence="10 15" id="KW-0408">Iron</keyword>
    <keyword evidence="11 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 15" id="KW-0520">NAD</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding" evidence="16">
        <location>
            <begin position="395"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU364066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6230345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="512" mass="56099" checksum="C40F4B42EA89C791" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL38</accession>
    <name>A0A8H6FL38_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Biogenesis of lysosome-related organelles complex 1 subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004875</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157788.1">
        <property type="nucleotide sequence ID" value="XM_037295787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL38"/>
    <dbReference type="GeneID" id="59333281"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009395">
        <property type="entry name" value="BLOC1S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13073:SF0">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13073">
        <property type="entry name" value="BLOC-1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06320">
        <property type="entry name" value="GCN5L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="120"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="162" mass="17413" checksum="BA21AF0D87A11D17" modified="2022-01-19" version="1">MTASASSATQVEQAHQQPPDPQRTAEARAAFTAHLTSIGNTHISALEGRVSDIHHNSAAIAKQEDDVSRQTRKLAAESRKHQKVADDAAEKLKELGDIQNWAEVLERDLLVLEETMKMGEAGEDGWETEDEGSVGEGGLGRATMVQGRGQRLNASEGYGPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYB0</accession>
    <name>A0A8H6KYB0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007177</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037298070.1"/>
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    <dbReference type="GeneID" id="59335577"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="1012"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="267"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="398"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="551"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="677"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="775"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="860"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="162"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="415"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="432"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="455"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="592"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
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        <location>
            <begin position="677"/>
            <end position="686"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="688"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="789"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="861"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="915"/>
            <end position="970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1236" mass="135530" checksum="668CF0F934476BD4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYG7</accession>
    <name>A0A8H6KYG7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_007151</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156679.1">
        <property type="nucleotide sequence ID" value="XM_037298044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYG7"/>
    <dbReference type="GeneID" id="59335551"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000018">
        <property type="entry name" value="S-adenosylmethionine mitochondrial carrier protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="10"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="101"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="200"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6229037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="288" mass="31085" checksum="484541432EC4CE0B" modified="2022-01-19" version="1">MESSTSKVESPFARALLAGALAGTTVDLSLYPIDTLKTRLQSSTGFYQSGGFRGIYAGVGSAVVGSAPGAALFFVSYESIKSVLKRAREVEAGKQWMGDWSEPIEHMAAASVGEVAACAVRVPTEVVKQRAQAKQQQNSLAALSFILGQRKDIGVMGVWREMYRGWGITVMREVPFTVIQFPLWEGLKEWRRRKTGKDGITALESGVFGSVAGAVAAAVTTPLDVLKTRLMLARDRERAGVLLVTILRESGPRAFFAGMGPRVLWISAGGAIFLGSYQWAYNAMGEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZL5</accession>
    <name>A0A8H6KZL5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004376</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157295.1">
        <property type="nucleotide sequence ID" value="XM_037295294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZL5"/>
    <dbReference type="GeneID" id="59332784"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF1">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="549"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="549"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="559"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="582"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="594"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="648"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6230038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="768" mass="83428" checksum="04D33C5501DA7D55" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C496</accession>
    <name>A0A8H6C496_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_006793</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146890.1">
        <property type="nucleotide sequence ID" value="XM_037297689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C496"/>
    <dbReference type="GeneID" id="59335193"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF29">
        <property type="entry name" value="BENZOATE-PARA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6217455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="22861" checksum="4CBFC17623D7095A" modified="2022-01-19" version="1">MSTFIIAGSDSIATNPGWTTYYLVQNPKIMKRLSEEIESAFTSEEQITPKSVCELPFMLRCLAETGRIYPTSLAGQSMVVPPAGDTICGKCVPGGAMVSLGVAEVLCQRNFADPTTYRPERWLGDPQYASDDRSVFQTFSVGPRSCIGKNLGNLETRLALDRMIWSFDMKLSADTDPASDDQTGFIPYQKKPLIVELRVKERSSIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7K5</accession>
    <name>A0A8H6C7K5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147581.1">
        <property type="nucleotide sequence ID" value="XM_037297007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7K5"/>
    <dbReference type="GeneID" id="59334509"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF270">
        <property type="entry name" value="O-ACYLTRANSFERASE AUSQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="458" mass="50397" checksum="4B92FECE033DB126" modified="2022-01-19" version="1">MLMPRMHVPKLLYFPSTAEPAAITSNLRAALALTLKSIPIIAGSVAPSKQEGVQKGSLSVQRPYFEAEDILSSNDLRNEYDYEQLRAAHFPSDEIAWDAIAPKQFGKAADSAPVMLAQANFLRGGLLLFFAVHHCVLDEVGLFNVMKVWSSSCRGDVDLHFVTPQWFDRSPLLQGAGTGRLEDHPEYALAPEGETVRAAEDPSVYFPSSENVHSAIFFFSDESLKRLKMVATMSGPNNGDDGSAWISTNDALIALFWCCITSARVAESTKATSEIFPLFAMAVNGRSQLHPPVAPDYCGNVVLIAKTLSKADDLLSPQPGCLAKAASLVRKAVNVVDDAYVRDVIQMVRNVDDVSRLAPRRRPSTEYSLGCSTWARQPYYSLDWGRTVGGACERIRKRRLHTDGLFLIFPRLPSTEGLDGKSDGRQGGVEVQLGLQSSHLQILMKNPVFMEFAQWRCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Z8</accession>
    <name>A0A8H6C8Z8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase C14 caspase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004927</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C14B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148537.1">
        <property type="nucleotide sequence ID" value="XM_037295839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8Z8"/>
    <dbReference type="GeneID" id="59333333"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050452">
        <property type="entry name" value="Metacaspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104:SF30">
        <property type="entry name" value="METACASPASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104">
        <property type="entry name" value="METACASPASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase C14 caspase" evidence="6">
        <location>
            <begin position="164"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="458" mass="49474" checksum="3E29FD94BA3F6336" modified="2022-01-19" version="1">MSFFPGQGQQHGYPPPQQYPPQGYGAPPPQGYGPPPPQGYGPPPQGYPPQNGYPPQGYSGYPPPQGGPSPQPGYGGYGPPPPQQPPRPYSGGYQPTPPPAGSGYGPPVPGGPQYGGRPGLPTTHTNAYTQGNPNAPPPPPQSQQSFGAGAPPQYSFQYSNCTGRRKALLIGINYFGQRGQLRGCINDVKNMSTYLNGNFGYAREDMVILTDDQQNPMSQPTKQNILRAMHWLVKDAKPNDSLFFHYSGHGGQTPDLDGDEDDGYDEVIYPVDFRVAGHIVDDEMHRIMVQTLMPGVRLTAIFDSCHSGSALDLPYIYSTQGVLKEPNLAKEAGQGLLSIVSQYARGDIGGIASTAMGFFKKATTGDEVYERNKVTKTSPADVIMWSGSKDDQTSQDAQIAGQATGAMSWAFISALKKNPQQSYVSLLNNIRDELESKYTQKPQLSCSHPLNTNLLYVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C979</accession>
    <name>A0A8H6C979_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-type proton ATPase catalytic subunit A</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_005560</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Vacuole membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148451.1">
        <property type="nucleotide sequence ID" value="XM_037296469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C979"/>
    <dbReference type="GeneID" id="59333966"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000052">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000003">
        <property type="entry name" value="Vacuolar ATP synthase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005725">
        <property type="entry name" value="ATPase_V1-cplx_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01042">
        <property type="entry name" value="V-ATPase_V1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="17">
        <location>
            <begin position="100"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="ATPsynthase alpha/beta subunit barrel-sandwich" evidence="18">
        <location>
            <begin position="203"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="16">
        <location>
            <begin position="309"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="19">
        <location>
            <begin position="542"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="38"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6219016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="690" mass="76057" checksum="9F24E784CBFF3776" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9L0</accession>
    <name>A0A8H6C9L0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Cytochrome b mRNA-processing protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_004024</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">Essential for the assembly of ubiquinol-cytochrome c reductase. It has a direct effect on the correct occurrence of the Rieske protein, core 4, core 5 and apocytochrome b.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CBP4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148990.1">
        <property type="nucleotide sequence ID" value="XM_037294943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9L0"/>
    <dbReference type="GeneID" id="59332433"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012420">
        <property type="entry name" value="Cbp4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28202">
        <property type="entry name" value="ASSEMBLY FACTOR CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28202:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07960">
        <property type="entry name" value="CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="115" mass="13615" checksum="A508B6E3BD984830" modified="2022-01-19" version="1">MSKAALWFKMLSTGVVICVGGPALVYWVSPTEEELFKKYNPELQKRSLENREQKQQDFDNFVNKLKEYSKSDKPIWIAAAEDEARQRANIAEEQRRIAQDIQRRREKIRDESLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9L7</accession>
    <name>A0A8H6C9L7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148880.1">
        <property type="nucleotide sequence ID" value="XM_037294833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9L7"/>
    <dbReference type="GeneID" id="59332322"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="158" mass="17093" checksum="3BFD043F364DCBF1" modified="2022-01-19" version="1">MSSAQKSAAQLREEASKAVMENGYHVLRRTFRVETDGTSPEPPFFPIPFLGLIQTDDLDRLPCDSERLELTGPHWSHLKDTGPYHDPVTGKSDVIVMKFSRKAGKTNAKWHMDIDKLSVLGGLGGVSVGGPGEGVRQGGISRQRRGSGSSSFVEAQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Q7</accession>
    <name>A0A8H6C9Q7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_004037</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149003.1">
        <property type="nucleotide sequence ID" value="XM_037294956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Q7"/>
    <dbReference type="GeneID" id="59332446"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08217">
        <property type="entry name" value="STKc_Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000697">
        <property type="entry name" value="G2-specific protein kinase nimA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000151">
        <property type="entry name" value="G2-specific protein kinase nimA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="10"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="432"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="563"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="704"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="336"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="456"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6219568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="738" mass="82328" checksum="BEC3EB5CBD93915A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA67</accession>
    <name>A0A8H6CA67_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148939.1">
        <property type="nucleotide sequence ID" value="XM_037294892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA67"/>
    <dbReference type="GeneID" id="59332382"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="124" mass="13563" checksum="3971E8A3F415C310" modified="2022-01-19" version="1">MTEWTKKKYNEAYTEYMPWIEDKVLGYWGENKTSYTAKDKLNTDVTGDKNVQAIQGGIAEGVGGQFSSGGLLGGVGDMTSKEGVNRAERGDPGLDPKELEKLNAQKKQKGWTETLSMGYMGGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAI0</accession>
    <name>A0A8H6CAI0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149178.1">
        <property type="nucleotide sequence ID" value="XM_037294490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAI0"/>
    <dbReference type="GeneID" id="59331979"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="115" mass="10641" checksum="9ED28483ECF2A7B5" modified="2022-01-19" version="1">MAPNDNLISRAVNNGVATAGSYAGGVVDAAGKSVSGAGRGVGDSVTNASNSAGGSASNYGNAVKDWTKAEGPRAQTASNPLGVSGGSYGGKKAVMGGGSKGGAGGKGTAGNPLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAS5</accession>
    <name>A0A8H6CAS5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-conjugating enzyme E2C-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149575.1">
        <property type="nucleotide sequence ID" value="XM_037294194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAS5"/>
    <dbReference type="GeneID" id="59331682"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019193">
        <property type="entry name" value="UBQ-conj_enz_E2-bd_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09814">
        <property type="entry name" value="HECT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="41255" checksum="C7DD67B2852DA997" modified="2022-01-19" version="1">MSSIYLYAELLLNIRRVTIFAILPSNCNAETRVWLCHDQRTLKLKHEDEEATIELPCLVVSDANLKIPPATTREISFRLFVSDAAKLPPQAKQATDCNDPWPAFKLTSETQVACGSCSTLLVNDVKVWKHLPSAGWADMMDFWHCHKPSVNISDDENAGNNKGYAAANALGPTAGIGVVDVSQLLISDIDCTGLETTDDQGLLNCVSCREIVGVKDKKDRYSLRLFKWSVTLQRSRGLDWETCSVQEIVSAQLLASIEDQAAYKFLAYTGEAEDSKPALVLWAFTPDLMYSTSAKPTQRAMKIFYRTITDSFKTLEKQRNRIEEFQLPSYALKILQASLKTSTDILPQSARTHREWTIGLLDRWSPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAY6</accession>
    <name>A0A8H6CAY6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149374.1">
        <property type="nucleotide sequence ID" value="XM_037294686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAY6"/>
    <dbReference type="GeneID" id="59332175"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="237" mass="25260" checksum="239D1AB640E6579F" modified="2022-01-19" version="1">MVQGLPAVDWKKPENNAKLFAAVLALIPGTPDYKKIAAVFGESFLIVTSSKATIVINYHIATALGLNFHKTKTIGTADGICYIGPNVPANAISYRLNVVRKEGVALGLSPGSGSFSTPTKINRAAKRAVPAATNNMLKGMGRKRGGMLSDGPSDDPDVAMNSEEEETAAETDDEKIPATPIPSVKRQKTVNGRVSKRISPRKGKKTDYKKLDDPFATMDNAKDENGNNVFGEPSGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB47</accession>
    <name>A0A8H6CB47_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149391.1">
        <property type="nucleotide sequence ID" value="XM_037294703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB47"/>
    <dbReference type="GeneID" id="59332192"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="222" mass="24975" checksum="BEAD5D8C40B4DDBF" modified="2022-01-19" version="1">MEQHPKASIPQQNLEMKPTAKSTIKPKALATRADWVNILWADISGQLRRSSVISADLFDGLVLKKDTATGSLILRELRLQYEELGHDRNLATVRVLVPQKSTYELWQLLWTIKRLRDPNGRQFHSEMAIDPIDRRMHLELDTYPGQIHSVTDFSLPSFIQDYGPGPSEFDPDPELDFDPTIGGIMTDAGAGTWVEEVPEADETDGGELDAKIDESKECFWGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBC9</accession>
    <name>A0A8H6CBC9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003436</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149739.1">
        <property type="nucleotide sequence ID" value="XM_037294358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBC9"/>
    <dbReference type="GeneID" id="59331847"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF158">
        <property type="entry name" value="MEMBRANE PROTEIN PTH11-LIKE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="61"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="428" mass="47088" checksum="4EEDCEEDADBE2802" modified="2022-01-19" version="1">MASTTISQPTPEQTSLSSDLPALPPPPGVTPNFTNPPNRGKLEIVVTSLLLCIATIFILNRLYMKTFIVRKYMLDDLTIVVGMVGTVTCYAAATFGKKPSSASSTTAHTPQTVGVHEGAIGVHLWDIKGQAGSRNTVISVYLISVLLSPTFLFIKITFFITYGHIFGPMRWMRICALLGGIFTTLFYIAMTVCFFIVVTPHRGQTWLSNTSTQREELNLHFGVPSSAVGLVIDIYLLVLPIVAVSKLQMATHRKLGIILIFTTGLLACLGSVLSIYYRVRLERTRDQTWALIPANTVTLLEMFVGIICACMPAASHTWQHHLPSYDSFKYHLRARNGTSGLKLGGTNPTPSSFAENNPRDVKTSRGVHEGYVNLDVLGHELPRPVKPLQTFIYSGRPDDLNHDGINLTYEMQQSSASPPPPKRGWTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCF4</accession>
    <name>A0A8H6CCF4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003079</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150081.1">
        <property type="nucleotide sequence ID" value="XM_037294003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCF4"/>
    <dbReference type="GeneID" id="59331491"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="158"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="545" mass="60544" checksum="E5D72BEA941A80DF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCS9</accession>
    <name>A0A8H6CCS9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150582.1">
        <property type="nucleotide sequence ID" value="XM_037292929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCS9"/>
    <dbReference type="GeneID" id="59330415"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF124">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="29268" checksum="2FD94DA0826924D9" modified="2022-01-19" version="1">MSTLNASAAASPSSQRLLDKTAIITGASSGLGRAIALTYATHGAAVVCADLRPVAEVHVKEGDTKATHEVILESGGKSLFVECDVRDSQSVQSLIARAVEVYGRLDIMVNNAGIGLEVPLLLPVHETPDSTFDTTMSVNSRGVFLGCKYAAQQMITQNPYPSGDRGWIINVASVAGVVGLGGTVSYSASKGSVVQITRTVALDLAPWRIHCNVLCPGFTKTAMIKPLTDVEATQARLGAMHPFKGLGEPEDIAKAALFLASDDASWVSGAALPVDGGYTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCX4</accession>
    <name>A0A8H6CCX4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002135</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150715.1">
        <property type="nucleotide sequence ID" value="XM_037293062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCX4"/>
    <dbReference type="GeneID" id="59330549"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07599">
        <property type="entry name" value="BAR_Rvs167p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000048">
        <property type="entry name" value="BAR adaptor protein RVS167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF1">
        <property type="entry name" value="REDUCED VIABILITY UPON STARVATION PROTEIN 167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="5">
        <location>
            <begin position="17"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="380"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="280"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="414"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="440"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="452"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="493"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="566" mass="62756" checksum="6421AC5B61A2AFA0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD61</accession>
    <name>A0A8H6CD61_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TECPR1-like DysF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002104</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150684.1">
        <property type="nucleotide sequence ID" value="XM_037293031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD61"/>
    <dbReference type="GeneID" id="59330518"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052816">
        <property type="entry name" value="Peroxisomal_Membrane_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="TECPR1-like DysF" evidence="7">
        <location>
            <begin position="103"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="15"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="484" mass="54841" checksum="0EE53844CCE8D0B2" modified="2022-01-19" version="1">MDEYTADAFVNREEPVPVIAVSGSDSPSSDPKGKRERLKESLGGSKDKLKEKLQDAGSGSKDYGYSLQDRLFAKLLQQVIPEEEVEELDLAPDVRSSKYINRPGFSLPLMTNNFRRFNARIGVVFVFQNRLERLLTWRKPTHTLSLLAVCTFVCLDPYLLSVIPVAAALLFVMVPAYLARHPPPPVSSIHTTSYSMNGPPLAPPRTIKPAAEMSKDFFRNMRDLQNCMDDFSTIHDAVLRIVTPPTNFSNEPLSSTIFLFLFVATCLLFITSHLLPWRLVCLLVCWTAIALGHPSIQQLLLSNHQKHLAPHERTAQSWLDAWISHDIVLDAPPETREVEIFELQRRNGGQNGEWESWMFSPGPYDPLSPQRISGDRPKGTRFFEDVAPPRGWEWGDKKWVLDLASREWVEDRMVQGVEVEIEGERWVGDLAGDKEAYEMKPIGKGKSKDLDEGGGDSKMGEWRRRRWVRMVRRKVVSDIGSIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDP2</accession>
    <name>A0A8H6CDP2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001604</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151073.1">
        <property type="nucleotide sequence ID" value="XM_037292534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDP2"/>
    <dbReference type="GeneID" id="59330020"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542">
        <property type="entry name" value="HELO DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542:SF3">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="84672" checksum="83FB082234428608" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF52</accession>
    <name>A0A8H6CF52_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001415</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151764.1">
        <property type="nucleotide sequence ID" value="XM_037292345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF52"/>
    <dbReference type="GeneID" id="59329831"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051972">
        <property type="entry name" value="Glutamate-rich_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903:SF1">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="5">
        <location>
            <begin position="87"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="300"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="400"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="494" mass="54861" checksum="2479FEDFD6505F45" modified="2022-01-19" version="1">MAKRPAEELGTEADALKGGERPLKVEDGEEMDFEDEFEDEYESEEDIFEAGVDGRPDEEREAEERRDAMDVDQQTFIPGRNQLGKGETLSPDSSAYEMLHTLSTTWPCLSFDIIKDSLGDNRKTYPATMYAVAGTQAESRKAKDNELLVMKLSGLSRMDRDNEGDSDEDDDEDDDENAEPILETKSIPVFSTTNRIRAYKVPQTDTSKPPTTFTATMTENAEVLIHNITPHLSSFDNPGTIITQQQSKPVSTLRMHRTEGYALDWSPLSPQGKLLTGDNDGKIFLTTCEGGKWTTDNRPFTGHTGSVEDIQWSPTERNVFASASSDGTVKVWDTRSKSRKPQLSVKISDTDVNVISWSQRNAFLLATGADDGVWSCWDLRQWKPSSPDGAAPTPKPVASFNFHKEQITSIEWHPTDDSIVAVAAGDNMLTLWDLAVELDDEESQNTADVKDVPPQLLFVHYMEMVKECHWHPQISGCVMGTGGGGFGVFKTISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF71</accession>
    <name>A0A8H6CF71_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001185</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151534.1">
        <property type="nucleotide sequence ID" value="XM_037292115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF71"/>
    <dbReference type="GeneID" id="59329601"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08282">
        <property type="entry name" value="PFDH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42813:SF3">
        <property type="entry name" value="GLUTATHIONE-INDEPENDENT FORMALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42813">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="9">
        <location>
            <begin position="30"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="8">
        <location>
            <begin position="190"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6222099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="389" mass="41319" checksum="3F5FF2EB4EE785C2" modified="2022-01-19" version="1">MSSTTMKAVHYEKPFQVSVREVPLPKIEHPDDAIIKVTTAAICGSDLHMYQGRTAAEAGLIFGHENMGIISEVGSGVTLLRKGDRVVLPFNVADGRCLNCEEGNTAFCTGVNPGFAGGAYGYVAMGPYAGGQAQYLRIPFADFNALPLPPGTAHEASFALLADIFPTGWHGITLSNFKPGEAIAVFGAGPVGLMAAYSAFLRGASRIYVVDKVPERLAAAKKIPGCTPVDFSAGEDAVDVIIRKNDGKMVDRSIDAVGYQAVGSGKEAGEVPNVVLEQCIKVTRPTGGIGVPGLYVPSDPGAVDEKSSQGMMLMSFGKLFEKGLTIGTGQCNVKKYNRYLRDMIISGRADPSFVVSHEIGIDEAPQAYEKFDKRVEGYTKVLIHPNGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFJ7</accession>
    <name>A0A8H6CFJ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="3">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001357</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151706.1">
        <property type="nucleotide sequence ID" value="XM_037292287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFJ7"/>
    <dbReference type="GeneID" id="59329773"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.620.20:FF:000004">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase subunit M2 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030475">
        <property type="entry name" value="RNR_small_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF18">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00368">
        <property type="entry name" value="RIBORED_SMALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="394"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6222271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="416" mass="47520" checksum="A689F0A32D00FCF4" modified="2022-01-19" version="1">MATQITPSKQASSGLENLKMGDSPAKKLKFEPVDKENVPVNIPAPVMDDLELKKPLVEVYDAKKEVTPTVAPTIKPEEADEPLLQENPHRFVLFPIKYHEIWQMYKKAEASFWTAEEIDLSKDLHDWNNRLNDDERYFISHVLAFFAASDGIVNENLVERFSGEVQIPEARCFYGFQIMMENIHSETYSLLIDTYIKEQKQRTYLFDAIDTIPCIRKKADWALKWISDKNSTFAQRLIAFAAVEGIFFSGSFASIFWLKKRGLMPGLTFSNELISRDEGMHTDFACLLFSHLNNRPSKQAVQDVITEAVAIEQEFLTEALPCALLGMNANLMKQYIEFVADRLLLALGNQKYYKATNPFDFMESISLAGKTNFFEKRVGDYQKAGVMASANKKEVVEGQEEKKNENGGDFNFDEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFR9</accession>
    <name>A0A8H6CFR9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000624</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151924.1">
        <property type="nucleotide sequence ID" value="XM_037291562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFR9"/>
    <dbReference type="GeneID" id="59329042"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048401">
        <property type="entry name" value="SLS1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048400">
        <property type="entry name" value="SLS1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20778">
        <property type="entry name" value="SLS1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20776">
        <property type="entry name" value="SLS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLS1 N-terminal" evidence="2">
        <location>
            <begin position="160"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="SLS1 C-terminal" evidence="3">
        <location>
            <begin position="468"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="579"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="937" mass="106087" checksum="12F4E610CC3519AF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGC1</accession>
    <name>A0A8H6CGC1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PXA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000731</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152030.1">
        <property type="nucleotide sequence ID" value="XM_037291668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGC1"/>
    <dbReference type="GeneID" id="59329149"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051837">
        <property type="entry name" value="SortingNexin/PXDomain-PKLike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999">
        <property type="entry name" value="PX SERINE/THREONINE KINASE PXK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999:SF23">
        <property type="entry name" value="SORTING NEXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="PXA" evidence="5">
        <location>
            <begin position="94"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="566" mass="62838" checksum="06ABADA6A3C2BA32" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGM2</accession>
    <name>A0A8H6CGM2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152180.1">
        <property type="nucleotide sequence ID" value="XM_037291818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGM2"/>
    <dbReference type="GeneID" id="59329303"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034690304" description="AB hydrolase-1 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="99"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="384" mass="41276" checksum="0F892B7C7FAA39E5" modified="2022-01-19" version="1">MYLPSLVASALLASSVVAKHCQNFSVPVTLCARNGAFNVPTLQDNHDATTFFANFTNPGGNFSNEVLLGYRTIDRTYDISVKFCQPDTGFGQDPTVQFLTHGIGFDKTYWDLPYNSYNYSYIDVAVDQYGFSTLSIDRLGIGNSSIADPLSIIQAPAEVSAIYELTMMLRRGSLPEVPRAFSKIVHVGHSLGSILSYNLAAMDPTASDGLILTAFSQDFAYIDATFDAWGSKLARNDQPQRFGDLPTGYLTWANASCNEKTFLYPGFFDPAMLQFSESTKEPLTTGEVLTLGGSPSTVPGFKGPVQVLVGNQDFIFCGGDCMATGDPALVGGSIPGGVTKAFPAAKAFEAYIQPNTGHAINLHYNATAAYKATQEFLIAQGLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGV0</accession>
    <name>A0A8H6CGV0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_001020</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152315.1">
        <property type="nucleotide sequence ID" value="XM_037291953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGV0"/>
    <dbReference type="GeneID" id="59329438"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6222969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="366" mass="39891" checksum="D4C1A77CAF77825F" modified="2022-01-19" version="1">MLAPAPLTEVAREDPKHEENGMSLEQTRKVALIATFFPKSRLTPPNLNVQATASSGDRDQYETKATRPSLPSYVGTFTSLLAQLVPQLDYKSTQHLTSHITQSTYTGLYQDLPDFSNIRHDAPIQDFERRHGLEIIFDVHPNASGHQQRPLLLALFDMDSTLINEEVIDELARSIGISDAVSAITARAMNGDIDFATSLSERVAMLKGVRTNVWEDLKKTVTIATGARELVSGLKKKGVLTGVVSGGFIPMAEWLKGELGLDVAVANHLLEEGPTADFPYPHLSGLLDSNYPVVTPELKRETLRSLAADHAIPLSETLAVGDGSNDLLMLGTAGLGIAWNAKEKVQTRAPMRLNGNSLADVLYLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGY6</accession>
    <name>A0A8H6CGY6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">eRF1/Pelota-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000222</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152597.1">
        <property type="nucleotide sequence ID" value="XM_037291162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGY6"/>
    <dbReference type="GeneID" id="59328641"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018444">
        <property type="term" value="C:translation release factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000006">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="434" mass="48675" checksum="2CC95D44C4BCB048" modified="2022-01-19" version="1">MSAPQANEAEKNIEIWKVKKLIKRLEQARGNGTSMISLIIPPKDQVSRAARMLAEEFGTASNIKSRVNRLSVLSAITSTQQRLKLYNKVPPNGLVVYCGEIITSEGKERKINIDFEPFKPINTSLYLCDNKFHTEALSELLESDQKFGFIIMDGNGALFGTLSGNTRDVIHKFSVDLPKKHGRGGQSALRFARLREEKRHNYVRKVAELAVQNFITQDKVNVAGLILAGSADFKNDLNQSDMFDNRLQSKVIKVVDVSYGGENGFNQAIELAAETLSNVKFIQEKKLIGKYFDEISLDSGKVCYGVEDTLKAMEASACETLIVFENLEITRWVLKSSTGSEVILHTNKGQEANREMFMDKETGQEMELVDQGSFLEWLAESYKDFGATLEFVSDRSSEGNQFVKGFGGIGAMLRYKLNFEQLAEVDSEDEFYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH92</accession>
    <name>A0A8H6CH92_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aminodeoxychorismate synthase</fullName>
            <ecNumber evidence="4">2.6.1.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Para-aminobenzoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">p-aminobenzoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_000068</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11672"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58406"/>
            <dbReference type="EC" id="2.6.1.85"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the anthranilate synthase component I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.85" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152443.1">
        <property type="nucleotide sequence ID" value="XM_037291008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH92"/>
    <dbReference type="GeneID" id="59328487"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00149"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046820">
        <property type="term" value="F:4-amino-4-deoxychorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008153">
        <property type="term" value="P:4-aminobenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF18">
        <property type="entry name" value="AMINODEOXYCHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="11">
        <location>
            <begin position="252"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="13">
        <location>
            <begin position="583"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="12">
        <location>
            <begin position="774"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="12">
        <location>
            <begin position="994"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="958"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1064"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="966"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1072"/>
            <end position="1084"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6223226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1140" mass="126956" checksum="F15C0C0F1DF66E99" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHE4</accession>
    <name>A0A8H6CHE4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152764.1">
        <property type="nucleotide sequence ID" value="XM_037291329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHE4"/>
    <dbReference type="GeneID" id="59328809"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="143" mass="15950" checksum="9614CE3EB8BA6AFF" modified="2022-01-19" version="1">MFERYDDFIGKGDAHSAAMMNVMIWGRGTQSHNDGRLSRVVADKLFDWCRMIAQREIAGNGGSAIPAEDLKPAAAGRLSDISVPSMVAYGRYDETSTNEAMKYAAQRIPGAKLKEFVIAHMINLESPSEFNNWLEAYLDQFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI92</accession>
    <name>A0A8H6CI92_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Uracil permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000494</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152868.1">
        <property type="nucleotide sequence ID" value="XM_037291433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI92"/>
    <dbReference type="GeneID" id="59328913"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000001">
        <property type="entry name" value="Uracil permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012681">
        <property type="entry name" value="NCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00800">
        <property type="entry name" value="ncs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF0">
        <property type="entry name" value="PURINE-URACIL PERMEASE NCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="555" mass="61137" checksum="0DDE6D052CC321DF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIP4</accession>
    <name>A0A8H6CIP4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7580 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010868</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153353.1">
        <property type="nucleotide sequence ID" value="XM_037301719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIP4"/>
    <dbReference type="GeneID" id="59339258"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1020">
        <property type="entry name" value="Prion-inhibition and propagation, HeLo domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056002">
        <property type="entry name" value="DUF7580"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038305">
        <property type="entry name" value="HeLo_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186:SF4">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24476">
        <property type="entry name" value="DUF7580"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034142247" description="DUF7580 domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7580" evidence="2">
        <location>
            <begin position="359"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="612" mass="68618" checksum="3B4AF60C8EEE3953" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIU6</accession>
    <name>A0A8H6CIU6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dilute domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010977</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037301826.1"/>
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    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
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        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037986">
        <property type="entry name" value="Myo5p-like_CBD_DIL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF6">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="170"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="203"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="3">
        <location>
            <begin position="387"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="655"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="803"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="662"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="686"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="824" mass="93229" checksum="55FE00C2F2CF5F22" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJF0</accession>
    <name>A0A8H6CJF0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010881</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153364.1">
        <property type="nucleotide sequence ID" value="XM_037301730.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF68">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01120)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="113"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="66"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="548" mass="59635" checksum="0BE6FEAE8E7F96FB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJI2</accession>
    <name>A0A8H6CJI2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009969</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153642.1">
        <property type="nucleotide sequence ID" value="XM_037300825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJI2"/>
    <dbReference type="GeneID" id="59338361"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="43"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="381" mass="42019" checksum="801D839559A31D15" modified="2022-01-19" version="1">MSTSAAAAPSAPPKIGQTSFQIAIGFLLGIALIVATGRTVIRARTYKSHGFTVDDGFFLLAVITFLAGTVMTYVDVPHFYSEELVEAGLESAPANFISSLILGEKLEDAVTSLLGVAIVSVKFSFLFFFRALLRQQKKMMVWWWCIFAILIPTAFLMIFAIFIVCAYWNQEIFVKCVTPAAHQRQNGVLKAITILDIVTDAFLISVPVLLLWNVRISIRRKLALCGILCLSVCTMIVSIIKVAGANTSTGGVDSSWVLLWYEIEAAVAIIVVSFTAFRALFVAHQALKYRTPAEDTSTSWNLWSRNTKGSRSKELPEIPSQVLGGVRTHIRRSRYGEGSFDNRGDDIELPLHGPGIIVTQRIHSEKTPRQPTTRPSAESFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJY9</accession>
    <name>A0A8H6CJY9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153867.1">
        <property type="nucleotide sequence ID" value="XM_037301050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJY9"/>
    <dbReference type="GeneID" id="59338587"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF32">
        <property type="entry name" value="PROTEIN KINASE COMPLEX COMPONENT, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G07660)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="325" mass="35492" checksum="B0B052FB21E29CD6" modified="2022-01-19" version="1">METINESHAAPDLATSLPQLPRPPSPSADRGIVPGTESSLTDSGTLPNADDIDLFTLSPVAALRMLCSTVETLVHITGDVPPTPPISPPSTPNLGLIQAEKESVARHTKEHRRRQSGGAENTGDVDGVPARAKTPIGSPEAGPSEPLHVIGSNMEPLNIQHGAIIRKFYSKKPPPISLEEYLLRLHRYCPMSTAVYLATSLYIFKLAVVERIIPVTSRNVHRLVLAGLRVAMKALEDFSYPHRRFAKVGGVGETELGRLEVSFCFVTNFELRVDREGLLEHAKSIRHRESLYRPQENLRPQLPTRDLVAEQSKPPTVTAETPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK87</accession>
    <name>A0A8H6CK87_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153789.1">
        <property type="nucleotide sequence ID" value="XM_037300972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK87"/>
    <dbReference type="GeneID" id="59338508"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="253" mass="27656" checksum="C7541C9312D75C17" modified="2022-01-19" version="1">MPSGGRGSGYTSNDSGADDPRSNGYRTGSYNQGRIRGNRNPGLVPAHADTTSRHRSMNIEDMLNPSDEDTRRYQQPQSSRSSDAGRTVSRNPGSLQGNRAPRSGTHSHGASASARSRGGSRPQPRRGQRSRGSGSPDPPLQTRSPRPDYTEEQEYFIWYLRIDCGYSWTAIADAYNARFSQDGGGGRKIPGLECKFYRITKANGLPNRPLDTRERHGLGLMADTQPVLPTVISLATKGAVDVRHTFTVLEPPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKA1</accession>
    <name>A0A8H6CKA1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010315</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153986.1">
        <property type="nucleotide sequence ID" value="XM_037301169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKA1"/>
    <dbReference type="GeneID" id="59338706"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF60">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID:OLEOYL-COA ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="37572" checksum="41408AC1D3E180FF" modified="2022-01-19" version="1">MEKYSQFRDRGSGIAPFFPIPTQSSGIYLPFHLFLLLIRLPILFTATLSYFFVLQWLPIGSLGKKASLWLILGAPGVWWIDLQIDGVKKGSLAKHHEARLPQPGSIIACSYTSPLDSLYLAAIFDPVFTASYPSTRLVHRISLFHAMFRAFQYPGLEPPKNEKLTDLQTLIQQYPGQAIVVYPECTTTNGRGILPFSPSLLTAPPEAKIFPISLRYTAGDITTPVPGSYVSFVWNLLSKPTHCIRVRIAESVHNTSQSPATRNSTSQTEREMLYDDRASSSDTLLNSEDAESMNAAEKRVLDKVAEALARLGRVKRVGLGLKDKDEFVKVWTKQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKS3</accession>
    <name>A0A8H6CKS3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA ligase</fullName>
            <ecNumber evidence="12">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_010120</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="12"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153793.1">
        <property type="nucleotide sequence ID" value="XM_037300976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKS3"/>
    <dbReference type="GeneID" id="59338512"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903461">
        <property type="term" value="P:Okazaki fragment processing involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000004">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000016">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 12" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="15">
        <location>
            <begin position="677"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="915"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6224926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="951" mass="105211" checksum="5EE5F4DE13E107D4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL88</accession>
    <name>A0A8H6CL88_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154156.1">
        <property type="nucleotide sequence ID" value="XM_037300308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL88"/>
    <dbReference type="GeneID" id="59337842"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031957">
        <property type="term" value="F:very long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF267">
        <property type="entry name" value="MALONATE--COA LIGASE ACSF3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="1">
        <location>
            <begin position="581"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="945" mass="104718" checksum="9844D70E1818DED1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLC4</accession>
    <name>A0A8H6CLC4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154199.1">
        <property type="nucleotide sequence ID" value="XM_037300351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLC4"/>
    <dbReference type="GeneID" id="59337885"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF382">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034626479" description="Amine oxidase domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="2">
        <location>
            <begin position="187"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="692" mass="77043" checksum="DE4FD0693BDBC1D7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLH7</accession>
    <name>A0A8H6CLH7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154289.1">
        <property type="nucleotide sequence ID" value="XM_037300441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLH7"/>
    <dbReference type="GeneID" id="59337975"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="103" mass="11641" checksum="04B71CB5F3FC9052" modified="2022-01-19" version="1">MAELLHAKMGGDTIDVPVPNLPVGTAGGWERVTLLKDEAYNDGEANRYHALGGWLILVGYMVDENGGLCRRSEEIREFSILRKGYGLWNKNETERRYFVMVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLP7</accession>
    <name>A0A8H6CLP7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154467.1">
        <property type="nucleotide sequence ID" value="XM_037300619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLP7"/>
    <dbReference type="GeneID" id="59338155"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006798">
        <property type="term" value="P:polyphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00144">
        <property type="entry name" value="MPP_PPP_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050126">
        <property type="entry name" value="Ap4A_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850:SF4">
        <property type="entry name" value="ZINC-DEPENDENT ENDOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="3">
        <location>
            <begin position="261"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="543" mass="61562" checksum="52B832E661A9C096" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLZ4</accession>
    <name>A0A8H6CLZ4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154587.1">
        <property type="nucleotide sequence ID" value="XM_037300739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLZ4"/>
    <dbReference type="GeneID" id="59338275"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="738" mass="82343" checksum="8339D5963B88D04B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM69</accession>
    <name>A0A8H6CM69_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009821</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154528.1">
        <property type="nucleotide sequence ID" value="XM_037300680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM69"/>
    <dbReference type="GeneID" id="59338216"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053230">
        <property type="entry name" value="Trans_reg_galc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47654:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47654">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="115"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="115"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="73"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="199"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="313"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="607"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="877"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="199"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="333"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="617"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="981" mass="110092" checksum="1CE25C482381C664" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMJ4</accession>
    <name>A0A8H6CMJ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Non-histone chromosomal protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009858</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NHP6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154565.1">
        <property type="nucleotide sequence ID" value="XM_037300717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMJ4"/>
    <dbReference type="GeneID" id="59338253"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01390">
        <property type="entry name" value="HMG-box_NHP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000016">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="8">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="6">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="72"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00057588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="105" mass="12022" checksum="1FE0FDB670B6C15F" modified="2022-01-19" version="1">MPKEKATRKTKAKAVSDGGKKKKDPNAPKRGLSAYMFFANEQRDNVREENPGISFGQVGKVLGERWKALNPKQREPYEAKAKTDKQRYEDEKASYNANDEEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMV3</accession>
    <name>A0A8H6CMV3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154916.1">
        <property type="nucleotide sequence ID" value="XM_037300090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMV3"/>
    <dbReference type="GeneID" id="59337624"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="20282" checksum="6804197C79F82CA2" modified="2022-01-19" version="1">MAGPDYGALGATFKIVRLLQVISLLGCIGMAANFISEMVSQNNTPSEELVGTLSVTCIAILYCAITVILHIDNILPYLINTGMDGLMLIALSVVAIVVGKPLSYLNCQVVGTSSVAESASQLSSQVKDTFNKQGGKVVYSNWIGANKTTCYEMKAVWGLSIALCILFAFSAMCSVCLWRRSKTVPPKDMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMZ4</accession>
    <name>A0A8H6CMZ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154976.1">
        <property type="nucleotide sequence ID" value="XM_037300150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMZ4"/>
    <dbReference type="GeneID" id="59337684"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1050.10:FF:000001">
        <property type="entry name" value="Putative Non-specific lipid-transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF25">
        <property type="entry name" value="SCP2 STEROL-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="428"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="537" mass="60506" checksum="EF5AE061CB8F8406" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNP0</accession>
    <name>A0A8H6CNP0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155171.1">
        <property type="nucleotide sequence ID" value="XM_037299170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNP0"/>
    <dbReference type="GeneID" id="59336700"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="362" mass="40625" checksum="4E3486FAB5093312" modified="2022-01-19" version="1">MGVSDMGDFQYQSIVNPSLLTNPDDSAGQGPFFNDMYPFGISEGDFADAPRPDATLDFDDFDFNQQALQSVDGGFDIPTTPQSSLADQRYVSFLPQSQTLPSSVWRPLEQPVQPFNYPDPEIFSQSPIDPTPYYPLQPLPSDNTMTQHNFGVITPPGQQFSSIDHPLFLHGNMGPLPATAGMFQYEPISNYQDDGAFNPEDLSLSAVPEDLVATAIREGWALPGYITQQSAPHDDLFVPNTGNDNDSDISDAPDSDEEYKPSSFAIAKRSRRSRPSQNKAILPNGEVKKGRPCAKPQTTERMRINERRMEGYYRRKYDQGNLEKARQQSKESYWRRKQRRIAAGEKVRSYNALKRGTSSKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP08</accession>
    <name>A0A8H6CP08_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RPEL repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008356</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155224.1">
        <property type="nucleotide sequence ID" value="XM_037299223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP08"/>
    <dbReference type="GeneID" id="59336753"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043451">
        <property type="entry name" value="Myocardin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004018">
        <property type="entry name" value="RPEL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22793:SF12">
        <property type="entry name" value="MYOCARDIN-RELATED TRANSCRIPTION FACTOR, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22793">
        <property type="entry name" value="MYOCARDIN-RELATED TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02755">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00707">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51073">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="86"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16004" checksum="C8D924EDCF2A97BC" modified="2022-01-19" version="1">MADENTAPSVDETPLSPIERQESLEKHLQMRPDAQDLKNRNILRDTHAAPAIQSAQLELERQQATDSLRKGLEKRPEKEELIGRNILPDSTAAPAIQGQQKELQKHMRADSLEQKLQQRPKPEELMKEGILDEDPTSPTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPI3</accession>
    <name>A0A8H6CPI3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-(3-amino-3-carboxypropyl)histidine synthase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008610</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DPH1/DPH2 family. DPH2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155476.1">
        <property type="nucleotide sequence ID" value="XM_037299475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPI3"/>
    <dbReference type="GeneID" id="59337005"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010014">
        <property type="entry name" value="DHP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00272">
        <property type="entry name" value="DPH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF2">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00408">
        <property type="entry name" value="Diphthamide_biosynthesis_famil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="420"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="476"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6227168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="557" mass="61939" checksum="004F9A34FF792E65" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ86</accession>
    <name>A0A8H6CQ86_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cell division control protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008779</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155644.1">
        <property type="nucleotide sequence ID" value="XM_037299643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ86"/>
    <dbReference type="GeneID" id="59337174"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000547">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016314">
        <property type="entry name" value="Cdc6/18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054425">
        <property type="entry name" value="Cdc6_ORC1-like_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22606">
        <property type="entry name" value="Cdc6-ORC-like_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001767">
        <property type="entry name" value="Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="198"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6227336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="627" mass="69129" checksum="50D0DC18B7813517" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQA9</accession>
    <name>A0A8H6CQA9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008724</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155589.1">
        <property type="nucleotide sequence ID" value="XM_037299588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQA9"/>
    <dbReference type="GeneID" id="59337119"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF29">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G06680)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="496"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="578" mass="63480" checksum="3D4749028B77F411" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQJ1</accession>
    <name>A0A8H6CQJ1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155724.1">
        <property type="nucleotide sequence ID" value="XM_037299723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQJ1"/>
    <dbReference type="GeneID" id="59337255"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="30190" checksum="E3AD3F3BEEFC8BC9" modified="2022-01-19" version="1">MSEPEMREPCEAFQKARTIKRHLKSCPGDEIPVIRPLSGHGVKVCGTCSKKNRNEQRKRENAQKRARRAKLKRNKMTAGIGAGNVVWTPPNTWPSIVVDSQTLALHSPQPKPLNDFNVRSMTQALQHGLNGSISTAMMAHNHVSVSQHERGVYSQSHQVFAATMSNALQLQEDGHDRIATEMRDDVTLHHQYKFESPDLPTTMLDVPVYQEQQCQHVGPGITMADDAKWKQSRTETCTFSEWSFRMELNEWSTVFDDNDDPDGAKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRJ4</accession>
    <name>A0A8H6CRJ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Elongin-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156212.1">
        <property type="nucleotide sequence ID" value="XM_037298876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRJ4"/>
    <dbReference type="GeneID" id="59336405"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051870">
        <property type="entry name" value="Elongin-A_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010684">
        <property type="entry name" value="RNA_pol_II_trans_fac_SIII_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141:SF76">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06881">
        <property type="entry name" value="Elongin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="43006" checksum="C63301A6B31A456C" modified="2022-01-19" version="1">MPPSSLFRMASRACIKNIRSITDVGDAPYQIVRSVLLRLENPQQLRKIELASPQICGQDGEIWLEFIKRDVPNWDKKPYEPENPKKWYKAYKKLVMDSQEEVENDAAELKATLDRIKQDQSLHKAQQVELRGVKVPEGMKSNGPVISLSTMPRFYDKGTNPPPGERQRKKDGDAKIFVSNKGKLDQFRKEAKAMGHFQKPAGRPGIFAPKDMKLKPAPMSRTITAAPRILIEEHQKAAAPSAFDPSAKPATIFAPKRKRVEHNETSQPGTLTYEEREKRLKAFTNPSSVSKSTLNQEASTPISPQPKAKIRSSTAPDIAILPVPKVQATPSFSSTLKRKDRASPTPGHGIPRANTSSPSNGGIRPAMHMKKAPVDVFMPAKRRKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS07</accession>
    <name>A0A8H6CS07_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 15">Protein disulfide-isomerase</fullName>
            <ecNumber evidence="5 15">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_008144</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="5 15"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156348.1">
        <property type="nucleotide sequence ID" value="XM_037299012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS07"/>
    <dbReference type="GeneID" id="59336541"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000139">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000154">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000185">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000017">
        <property type="entry name" value="Protein disulfide-isomerase A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF132">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11 13" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034842264" description="Protein disulfide-isomerase" evidence="15">
        <location>
            <begin position="21"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="17">
        <location>
            <begin position="9"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="17">
        <location>
            <begin position="335"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="487"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="488"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="516"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="13">
        <location>
            <begin position="50"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="13">
        <location>
            <begin position="385"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605792-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6228414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="527" mass="57000" checksum="AFFC931FB625447D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSL5</accession>
    <name>A0A8H6CSL5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin carboxyl-terminal hydrolase 14</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Deubiquitinating enzyme 14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin thioesterase 14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquitin-specific-processing protease 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_008181</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156385.1">
        <property type="nucleotide sequence ID" value="XM_037299049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSL5"/>
    <dbReference type="GeneID" id="59336578"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14385">
        <property type="entry name" value="UBA1_spUBP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14386">
        <property type="entry name" value="UBA2_UBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000086">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000103">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000396">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000587">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000235">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000144">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF664">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="20">
        <location>
            <begin position="468"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="19">
        <location>
            <begin position="619"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="18">
        <location>
            <begin position="907"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="18">
        <location>
            <begin position="972"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6228451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1113" mass="121463" checksum="70FF90E968A11B86" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSP8</accession>
    <name>A0A8H6CSP8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008226</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156430.1">
        <property type="nucleotide sequence ID" value="XM_037299094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSP8"/>
    <dbReference type="GeneID" id="59336623"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="424"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="452"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="68"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6228496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="507" mass="56209" checksum="0A82B1F2905A2BE8" modified="2022-01-19" version="1">MADEKVPSYPDVEQTLFDDKDGAPPAQVLKHANDADEAMKAFEDHDGELLVLDEATNRRLLRKIDLNILPLLCVVYGLNYLDKTTLSYASVMGIKKDIGLVNDDYQWLGSMFYFGYLAWEYPTNRLLQRLPLAKYSSFCIILWGLVLCCMAAVKNFSGAIAVRFFLGIFEAAVTPGFALFSSQWYTKKEQGTRIGIWFSFNGFAQIFGGLVAYGIAKGAALHGSSIAPWKIVFLVTGLLTATIGCLFLFFVPDNQLNARWLSKNDRRLAVERVRVNQQGIGNKHFKAYQLKEALLDPLTWAMVFFALTGDIPNGGISNFFSQLIVSFGYTADQSLLYGTPGGAVEVVSLIVCGYLGDRLGNRILVSTSGLMISILGMILIVALPLSNDGGRLAGYYLTQASPTGFVALLSLISSNVAGYTKKTTVAALYLIGYCVGNIIGPQTFRPKDAPRYVPAEITIICCWAACLVDLLFIWWYCQNQNKKKAAFRAQPQYRKLENQEWLDLTDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSS9</accession>
    <name>A0A8H6CSS9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156552.1">
        <property type="nucleotide sequence ID" value="XM_037297917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSS9"/>
    <dbReference type="GeneID" id="59335422"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="111" mass="12296" checksum="4A3C565EFB013BCD" modified="2022-01-19" version="1">MGGPSSSASGGTPNADGQDDHKMRQSEEGRVRVGTVFSSGAVYAKVVRHRNGTQKITYHCPDTEIEVVGEDIVWLREVLAFRERFPTTSVPADKLDSCMALAYMTWVKYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6T2</accession>
    <name>A0A8H6F6T2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147105.1">
        <property type="nucleotide sequence ID" value="XM_037297669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6T2"/>
    <dbReference type="GeneID" id="59335173"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="208" mass="22406" checksum="B7285AF70B6D4567" modified="2022-01-19" version="1">MASEPDDYQTISDNCPGYMVLISEGIICTRCAGEKRHRPNDCPKPTTAADLATETFLEGDATGDDNADPGMESLSDTSSMFSESTARSESSEMQVTDAHQSDQGGSHQQDTHPPSRGFARADGSHTDTEPDRTSPSPPNRRAEFRVPKVPLNEPSRAPKIFVKANRQTSNTATTHKSALANTTGKFDYDNTTCPSSFGFGRLPSEAMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F827</accession>
    <name>A0A8H6F827_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147871.1">
        <property type="nucleotide sequence ID" value="XM_037296690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F827"/>
    <dbReference type="GeneID" id="59334190"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="2">
        <location>
            <begin position="2"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="273" mass="30403" checksum="00D8A327F22503B8" modified="2022-01-19" version="1">MVKFYESSFNYDYPLPAVSLAYFLRYPNPYATHVLSTDVIDRHFDPATQRLSTTRLHLKKSKLPPTVLKLLPKGILGSSADAGQSYILEKSVIDVKEGWMKTESRNLEWTGILSVVEKQVYRRPSSGTATPEIPRLDLVESTKGEGTDVTTTVTLLSRFGQAKTLRKKGEVGAPIPLPASDSEEESPPKKGFFSSWSTSSLQRSIEMIGLRRTREATGKSNEGMKVVLERLRGGGLGRVLEGMQEDRQRAFGQDPGSSFKRAWRQEQDSSSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F887</accession>
    <name>A0A8H6F887_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005368</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148260.1">
        <property type="nucleotide sequence ID" value="XM_037296278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F887"/>
    <dbReference type="GeneID" id="59333774"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="259" mass="28574" checksum="3CE619830AA452DC" modified="2022-01-19" version="1">MSKPSSSSKAPSTVPGVNLSFYVQIDPLEPNYQAEPARHGTHNFWAPPHTKTGYNRKPGMLFRWGSGVITHVSANDTVGRSLHQTYSAATVFTQNPDTPHLLAVPFDAETKHARENQGGWRPLFFRHIQNTQHTYSAVSTVGDRQHIAAPGSSHWMPQLLPRVYDYQAGSPRIQAGLIGSIPLLIALAAFSAPPDALREVLINCLRPGTWRPHRFQYPAGHIAERGMVVTIFFDPVNPQGSNAVMLNKLQNGDFGAFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8E0</accession>
    <name>A0A8H6F8E0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005534</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148425.1">
        <property type="nucleotide sequence ID" value="XM_037296443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8E0"/>
    <dbReference type="GeneID" id="59333940"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF68">
        <property type="entry name" value="GLYCOSIDASE CRH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034803600" description="GH16 domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="853"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="3">
        <location>
            <begin position="58"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="878" mass="89794" checksum="8220628A52F6FC6A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8L4</accession>
    <name>A0A8H6F8L4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148441.1">
        <property type="nucleotide sequence ID" value="XM_037296459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8L4"/>
    <dbReference type="GeneID" id="59333956"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="23154" checksum="97713880CA9A5933" modified="2022-01-19" version="1">MNDHASSSRSPPKTSADADRSSDESASVSLEQELVAWGDGYQAWVDRNLQSLLKDFVTGKKSKIGVAMCPNTVTRCIAKEGTDEDLRSILGKIYQKYGPIRMYYKGCDTLANHWVTGWETVTGAPDVQRVMICVEMSDRPPVSEKVAAMIKANVEKFRQDVRDRVNWVDVPSDSVDHGDWIEPDGIRSTVKNVGRFLLDGLEAFRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F919</accession>
    <name>A0A8H6F919_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148544.1">
        <property type="nucleotide sequence ID" value="XM_037295846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F919"/>
    <dbReference type="GeneID" id="59333340"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF3">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G09260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="117"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="355" mass="39723" checksum="46DD92E7269A5AF5" modified="2022-01-19" version="1">MAAESSASPADSKKPALVSQPSVANEHQAKAFAELKALCENNKLYWPASEIEGYPAEGHNDDKDLLRFLVARSYDPQAAYKQYSASAAWRRKVALVQTYDNVEIQRFARMNRVQPQWTGRRDRMGIPLMVYVLSEIRPEDLAQISKEDPGLSSIFLPAEYITNFTQRLCNRVRNDGTIINKSVNIIDLSNVGLRKFWNLRSILQTASGMATAHYPETVERIFVVGAPSFFPTVWSLVTSRFDPATTKKIFVLSASEATEKLTEFIAPEDLPRRFGGTLDWTFPMRPVPDAETKEIIGGLADRWVEGPIRYLSKPEGDVLVAVGSQDGQLRRETLAHIPQGSSSRNGQPSEHVPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9C9</accession>
    <name>A0A8H6F9C9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003997</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148963.1">
        <property type="nucleotide sequence ID" value="XM_037294916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9C9"/>
    <dbReference type="GeneID" id="59332406"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="77" mass="8677" checksum="DD8D0FFA8F42F231" modified="2022-01-19" version="1">MDFISISAVYYHAPRTENVSTILWSEAPEAKGNPHHPVQKIMGMDLGQRAQDKVLVQRHISNGPIEEINPDQPLTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9E5</accession>
    <name>A0A8H6F9E5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_003293</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149597.1">
        <property type="nucleotide sequence ID" value="XM_037294216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9E5"/>
    <dbReference type="GeneID" id="59331704"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000345">
        <property type="entry name" value="Mechanosensitive ion channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058650">
        <property type="entry name" value="Msy1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323:SF15">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25886">
        <property type="entry name" value="Msy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="493"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="437"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="736"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="810"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="939"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="831"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6220162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="989" mass="110835" checksum="A8B7086289C66D01" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9J9</accession>
    <name>A0A8H6F9J9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149099.1">
        <property type="nucleotide sequence ID" value="XM_037295052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9J9"/>
    <dbReference type="GeneID" id="59332542"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="241" mass="26379" checksum="7CC4E13A151E2C2E" modified="2022-01-19" version="1">MAQIASPKLELLRAKLALAIGPVEINLAYDEVKAARPNALDEPWSEDMSGPHHHVGKLLSHHSLNNWGLTSLMLVFNNKAFVLSEVANEQLASGMARGCPSIDAGENIKLYLNDIWQLEVFRMMGLDLAAIRCPITGRDPLGSLYYSLITAVNERILKQKFPVNGSEVIDGMANLGIDDVALEEKGDEAKGGEWSEEEISSEGGQEKEDGDQAAQDREKNTVKQTSVEEEKQAHADAVVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9N3</accession>
    <name>A0A8H6F9N3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TMEM1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003668</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149278.1">
        <property type="nucleotide sequence ID" value="XM_037294590.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332079"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055505">
        <property type="entry name" value="DUF7077"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056916">
        <property type="entry name" value="NTS_TR130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045126">
        <property type="entry name" value="TRAPPC10/Trs130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022233">
        <property type="entry name" value="TRAPPC10/Trs130_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056913">
        <property type="entry name" value="TRAPPC10/Trs130_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251">
        <property type="entry name" value="EPILEPSY HOLOPROSENCEPHALY CANDIDATE 1/TMEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23274">
        <property type="entry name" value="DUF7077"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24967">
        <property type="entry name" value="NTS_TR130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12584">
        <property type="entry name" value="TRAPPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23036">
        <property type="entry name" value="TRAPPC10_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24965">
        <property type="entry name" value="TRS130_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="6">
        <location>
            <begin position="32"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Trs130 NTS" evidence="8">
        <location>
            <begin position="698"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7077" evidence="7">
        <location>
            <begin position="972"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 C-terminal" evidence="5">
        <location>
            <begin position="1303"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1528" mass="170390" checksum="4B64EA34DCDD6F56" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9S4</accession>
    <name>A0A8H6F9S4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD linked oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003788</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149398.1">
        <property type="nucleotide sequence ID" value="XM_037294710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9S4"/>
    <dbReference type="GeneID" id="59332199"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF39">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034362205" description="FAD linked oxidase N-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="FAD linked oxidase N-terminal" evidence="7">
        <location>
            <begin position="81"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="169" mass="18049" checksum="1822146DF2C978C4" modified="2022-01-19" version="1">MHTSLQVIVTLLNLVPLIAAIPSFIGHTRSDNFTRLPPSVSTYQQELGPRLSKNATLYFPNSSGYINDTERWAANTKSNFSVVVVPGTAQDVGATVSFADTYNVPFLAVNKGHGGAAGLATVYNGIQIYVRALDDIEVSEDGNSASMGGGVYQDQLVNYLFDRGKAACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAJ1</accession>
    <name>A0A8H6FAJ1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002217</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150797.1">
        <property type="nucleotide sequence ID" value="XM_037293144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAJ1"/>
    <dbReference type="GeneID" id="59330631"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.370.10:FF:000001">
        <property type="entry name" value="COX11 cytochrome c oxidase assembly homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00155">
        <property type="entry name" value="CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003465">
        <property type="entry name" value="PRK05089.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005413">
        <property type="entry name" value="COX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="205" mass="22990" checksum="B01E1250EF7A5F78" modified="2022-01-19" version="1">MEQLRAPFSHKNSSTLYYTLSVILGTVALSYGSVPMYKMICQQTGWGGQPIKASIHSNAEDPATRLTPVTDSRRLRITFNGSVSDIMPWKFTPQQREVRVLPGETALAFYTATNKSTEDIIGVATYSVTPAQVAPYFSKIQCFCFEEQRLNAGETVDMPVFFFIDPDFVNDPTMTKIDTITLSYTFFKARYDKNGNLTPMPMTMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAL7</accession>
    <name>A0A8H6FAL7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002742</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150496.1">
        <property type="nucleotide sequence ID" value="XM_037293667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAL7"/>
    <dbReference type="GeneID" id="59331154"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39596">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39596:SF2">
        <property type="entry name" value="HET DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G17550)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="38984" checksum="5A15B99141999EF8" modified="2022-01-19" version="1">MSPEDAIEECNCQIDPFDISSHVATILDDGDIPLISVSIEGKPNISRTSVHQGQSTCEFVALSHVRSQNLFLDGTSKCSLQQLQSLVNELLQSKILLSLGTTLPFWIDTICIPEDHRKKTATEKIKRVFEAADAVLVLESSLYRQRPGSISDYIAAIRCSPWTKRLWTLQEAALAKRLCFSFHETVVLLDDLNVSQEAAESVTAVERVFRTGNTKEVREKLDCLDLLDADIKSLFAAPINVSHTSAKDVVKEDKPEVASEANWNGTKRLHLVSVLRLGYFSLPRFYLIRDVNENSESQAVMEQIMTLYKTEYSAGRGMKTSMSMEMRLEVLVAFKLNRPSKNNHMID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAN3</accession>
    <name>A0A8H6FAN3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="18">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_002787</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="17">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports the calcium to the vacuole and participates in the control of the cytosolic free calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="18"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150541.1">
        <property type="nucleotide sequence ID" value="XM_037293712.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331199"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000028">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000031">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000018">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 18" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="506"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="964"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="997"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1048"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1112"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1145"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1292"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="224"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="20">
        <location>
            <begin position="330"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="21">
        <location>
            <begin position="991"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="583"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1234"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1311"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="591"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1237"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1332"/>
            <end position="1341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00038148"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAF6221106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1388" mass="151836" checksum="3400C8C761DEA789" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAV8</accession>
    <name>A0A8H6FAV8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002799</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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            <end position="499"/>
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    <feature type="transmembrane region" description="Helical" evidence="3">
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="64976" checksum="59E4646AE9250FDD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8H6FB90_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Asl1-like glycosyl hydrolase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">HO133_001183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151532.1">
        <property type="nucleotide sequence ID" value="XM_037292113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB90"/>
    <dbReference type="GeneID" id="59329599"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071966">
        <property type="term" value="P:fungal-type cell wall polysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024655">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="151"/>
            <end position="370"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="373" mass="39468" checksum="9014D8992D0F87C0" modified="2022-01-19" version="1">MDQEVCADDYMLQKDHVLVGSSPRAASARLGGVSAPVSQSGGVSGYDGKDQSCDASPETSSADDTVDPTEEKDSQQAASTSSDTSTAGGADTAAAPVEKTSTSDSTSTDHSSNNIDSSNSGTNNSTGAAGASVSSRKGAGLWYFKEIATMTSDIHAPWAYDWSDDPLENSFKGTAPAGVDLVAMFDSTSDYSSLASSSYKEVVGWNEPDATAAPVDSAAAAQVWPNIVKTVGKRMGSPAPASTSLTQGDWFYDFMTSVIKAGSPPDFICLHYYSPNGDVSKFQSYIEGVYNMYKLPTWVTEWAYVDYSKDPATVPSTAEQVAYMQAAVKMMDELSYIERFAWFAVPWSSVQPASSLFDEFGSITPMGTAYAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBN5</accession>
    <name>A0A8H6FBN5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151378.1">
        <property type="nucleotide sequence ID" value="XM_037292839.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330325"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="760"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="760"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="943"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="967"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="980"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1057"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1133"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1182"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1292"/>
            <end position="1304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1336" mass="145957" checksum="A93783396D1646CC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC33</accession>
    <name>A0A8H6FC33_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tim44-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000971</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152266.1">
        <property type="nucleotide sequence ID" value="XM_037291904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC33"/>
    <dbReference type="GeneID" id="59329389"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024621">
        <property type="entry name" value="Mba1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051975">
        <property type="entry name" value="mtLSU_mL45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28554">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L45, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28554:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07961">
        <property type="entry name" value="MBA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25705" checksum="4EE9A9064F5EFE1E" modified="2022-01-19" version="1">MQRIRNDMGLLPGTFVMPSGLIKPSLLRTPNLRMRLEWYRLKKKWRALQGCITYKFLLKSRPWPKLRRGTIAPEAKRLYETMYTAFADGDMATLESICHEGLLASFGTRINVRPPKESLQWTLHKYIGFSRIVSTNIVSLGIEESALYQVVVKIRSMQSLERTPTNGVASDTTEAKKMVEYVVLQRMMLRGKEGRWKIWGTIEESKVEDVLGDDAVVATPVVGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCE3</accession>
    <name>A0A8H6FCE3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152312.1">
        <property type="nucleotide sequence ID" value="XM_037291950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCE3"/>
    <dbReference type="GeneID" id="59329435"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="76" mass="7159" checksum="CA7DCCF8C5A68F30" modified="2022-01-19" version="1">MAKCCKAKLDAYAAAEQSPFSITNRNRSAAGVRAGPRSGGSGGGGGGGGNGGSGGGGGRKVGTVDDIRGPECKSCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCG6</accession>
    <name>A0A8H6FCG6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell division cycle protein 123</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC123 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152559.1">
        <property type="nucleotide sequence ID" value="XM_037291124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCG6"/>
    <dbReference type="GeneID" id="59328603"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009772">
        <property type="entry name" value="CDC123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323:SF6">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 123 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323">
        <property type="entry name" value="D123 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07065">
        <property type="entry name" value="D123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="328"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="48973" checksum="4B6EED6B0A45F519" modified="2022-01-19" version="1">MPIANADLMERSPSLEPTSEPLMELPFPVVTKSHILNCSYHSWYPRYRSLTPKARLIPLSASFLSYLRADGIILPPEDIPSSTGSDTDNEEEEEDQTTDPSQAWLDMHQKIKSTISELGGSVMPKLNWSAPKDATWISATNSMTCTTPNDIYLLLKSSDFITHDLEQVLDDCEDESDDESEVENQDEERSASEHLSKIPYHLILRKTIPAFNPALEIRCFVRSRHLLCMCQRDLNHFDFLPRLIPKLRSLIQDAFDKNLKRTFPDESFVFDVYIPPPHDRVWLIDVNPWAPRTDPLLFSWLEVLNMRGEEQGGADNLQDGVVRLSLNGNGSVDRGSDLDDEQEEEVEEASEDEEEVFTPEFRLVNRDDPEAYNFNTPQYSAHKLPKDVVDASTDGEAGLREFMGTWREIVARQEKEDREEKDNGIVDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCI8</accession>
    <name>A0A8H6FCI8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152190.1">
        <property type="nucleotide sequence ID" value="XM_037291828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCI8"/>
    <dbReference type="GeneID" id="59329313"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37919:SF2">
        <property type="entry name" value="EXPERA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37919">
        <property type="entry name" value="PROTEIN CBG05606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="110" mass="11829" checksum="CA7489FE065F5933" modified="2022-01-19" version="1">MAPGGAWHAPLFVPYALYGRVDRLYGAGAWEARNGFTGAQAGLNVVESVGYAAYLWVVWRYGEEDDGGKRVVGGGYMAYEFARDILHGLSIASGDRSSKPARAATPTKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCP9</accession>
    <name>A0A8H6FCP9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152399.1">
        <property type="nucleotide sequence ID" value="XM_037292037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCP9"/>
    <dbReference type="GeneID" id="59329523"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021514">
        <property type="entry name" value="DUF3176"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394:SF5">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11374">
        <property type="entry name" value="DUF3176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="606"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="713" mass="77731" checksum="85D77C99BF5A08B5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCS7</accession>
    <name>A0A8H6FCS7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152513.1">
        <property type="nucleotide sequence ID" value="XM_037291078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCS7"/>
    <dbReference type="GeneID" id="59328557"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034917205" evidence="2">
        <location>
            <begin position="18"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="365" mass="40224" checksum="5DD1668BF1A5B28E" modified="2022-01-19" version="1">MLLPITFLSALIALSTTLTVPTSPHIYRATQSNSSSFSPLPLLKPLPIKSDPTTSPNTLNLYSQANTTTPPPPPTIVIWPQLPFTTDTGRHLNLTIARLGPITSRTRRGELLNAISDLHHAVAAEGDPFEPISGVYARSSGIVWTVFACRGEVAMKRFQVAQVLSMVWILMEDYGPREVVGSEIVSAEGRGLASFVLRFLGGTDLKKLQRKMMREDRDADEETQLEFPKRYLSLLSLAFYRLVDYNEDIQWAMLVALKSVNVKARDLATAQALEAEGITVDSTMAQSWPAWKDDQPLKRKKGETTIAGDQARAAPHEEKEDVAPGDATDGQNKPDPSNGLDGLFDHDYGDHDGYDGSDFFDMEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD31</accession>
    <name>A0A8H6FD31_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">intramembrane prenyl-peptidase Rce1</fullName>
            <ecNumber evidence="10">3.4.26.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000272</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152646.1">
        <property type="nucleotide sequence ID" value="XM_037291211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD31"/>
    <dbReference type="GeneID" id="59328691"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="12">
        <location>
            <begin position="152"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6223429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="338" mass="37854" checksum="3B8841BF7D6C0125" modified="2022-01-19" version="1">MAPTSFYQRLSNYVRESDKVEPPMLSASAAAALSVAYTVLYVLPFYLSSTTRPSPTLSRDAPSVIRARIRFVTGSVFICSALTIYVISTRAEVSYSEIFHLLGWYPFSPLDIPKVLSLTALLFAGPLFEKGVIESGWRHWAKGRDLHETLSSWIGWRNYIAGPFTEEVLFRSLLLSLHLLIRPPSSQTILIFVTPLYFGIAHIHHFYEYTLTHPFTPLPPALLRSIVQFGYTTVFGWYAAFLLLRTGNLWGVVVVHGFCNWVGLPRVWGRVGGVVIDGGVVGGPVRGKEDEDRRDEGAQKLGPAWTAAYYIILVAGVVAWWKALWVLSESERELMKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD92</accession>
    <name>A0A8H6FD92_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153150.1">
        <property type="nucleotide sequence ID" value="XM_037301516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD92"/>
    <dbReference type="GeneID" id="59339054"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="683" mass="74783" checksum="D1CEB242ED8E8079" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE12</accession>
    <name>A0A8H6FE12_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Life-span regulatory factor-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153767.1">
        <property type="nucleotide sequence ID" value="XM_037300950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE12"/>
    <dbReference type="GeneID" id="59338486"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024368">
        <property type="entry name" value="Ecl1/2/3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12855">
        <property type="entry name" value="Ecl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="34872" checksum="9E2B8A80ADEA0FAC" modified="2022-01-19" version="1">MSSSHHAQVHVTRRSPPNVRKVYVKAPPPVPSSKAPVTPKITIKVAKSTKHPKDDEDDTFQDDEDDDMASSFLQFCAMCEKQILVPNSSILYCSESCRRRDSVVPTTNTYLSLASPTKMVPSAYSDDIDDPFGSKIPTYVAAMQPTPRPTSDARIPPVAHNGKSDLDPTEWKPAEPILTETKSPPSTKAGEWKPKLPHRPSSEAFSYLSKFHRSSDSLYSKRRPALSHGRSTISSRTTPSLSHTPTASTSSEESLSGTPYEFVARPALSMPTTTASHCRDGGKLAQVEEHDLTYEKKFVGTSLGSATGSLKKLLRTGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEQ1</accession>
    <name>A0A8H6FEQ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation machinery associated TMA7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154481.1">
        <property type="nucleotide sequence ID" value="XM_037300633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEQ1"/>
    <dbReference type="GeneID" id="59338169"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015157">
        <property type="entry name" value="TMA7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28632">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09072">
        <property type="entry name" value="TMA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7338" checksum="29BC2FED4B6C4FC2" modified="2022-01-19" version="1">MGGNSREGGKAKPLKAPKKEKKDLDEDEVAYREKQKADAKAQKEMAEKAKGKGPLNAGQQGIKKSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFE2</accession>
    <name>A0A8H6FFE2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154878.1">
        <property type="nucleotide sequence ID" value="XM_037300052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFE2"/>
    <dbReference type="GeneID" id="59337586"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="245" mass="27791" checksum="CE5C4C975B157F0F" modified="2022-01-19" version="1">MVLKRLFALFFRPDETKSASTSGQRATSERGPSEETDERRPATTPSADNYVRLCPHETLSFKRAQRILSLYGQGDIHPTMDAFTSAPQLHHSELSGGEFGRRKHPRLCKQDPSGYDVLQATSDYGFEKGLVLNIRWQLSFKAHPELLKSVECLQQFLDSCDISLCPHKNLSDEEIAFKTFYSLKPPTDPIEKFEIASMKIKGCDQCGTDFWVSGRDDKKCRVETFRSLGTIETVDDKQWLAQCSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGA2</accession>
    <name>A0A8H6FGA2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">26S proteasome regulatory subunit RPN2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008472</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the proteasome subunit S1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155339.1">
        <property type="nucleotide sequence ID" value="XM_037299338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGA2"/>
    <dbReference type="GeneID" id="59336868"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000017">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016642">
        <property type="entry name" value="26S_Psome_Rpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048570">
        <property type="entry name" value="PSMD1_RPN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040623">
        <property type="entry name" value="RPN2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18004">
        <property type="entry name" value="RPN2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21505">
        <property type="entry name" value="RPN2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015947">
        <property type="entry name" value="26S_Psome_Rpn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal" evidence="9">
        <location>
            <begin position="192"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome regulatory subunit RPN2 C-terminal" evidence="8">
        <location>
            <begin position="889"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="351"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="909"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1089"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="920"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="949"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="969"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1102"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1127"/>
            <end position="1139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6227031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1139" mass="124084" checksum="C9DA7DB5D6FAF42D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGD3</accession>
    <name>A0A8H6FGD3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155429.1">
        <property type="nucleotide sequence ID" value="XM_037299428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGD3"/>
    <dbReference type="GeneID" id="59336958"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="460"/>
        </location>
    </feature>
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        <location>
            <begin position="477"/>
            <end position="663"/>
        </location>
    </feature>
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        <location>
            <begin position="699"/>
            <end position="762"/>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
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        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
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        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
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        <location>
            <begin position="178"/>
            <end position="215"/>
        </location>
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        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
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        <location>
            <begin position="545"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="595"/>
        </location>
    </feature>
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        <location>
            <begin position="722"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="762" mass="84365" checksum="0B60F42D0442A9FC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGI7</accession>
    <name>A0A8H6FGI7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155609.1">
        <property type="nucleotide sequence ID" value="XM_037299608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGI7"/>
    <dbReference type="GeneID" id="59337139"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012479">
        <property type="entry name" value="SAP30BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13464:SF0">
        <property type="entry name" value="SAP30-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13464">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN HCNGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07818">
        <property type="entry name" value="HCNGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="374" mass="40399" checksum="0A80822E481C4F9A" modified="2022-01-19" version="1">MTGLVGYGSSDEEDSVDESGRMQSKSQTPTQSDLSVSGGQRPPNNTRNEPTAVPAEDEALHPARPNGTVLGPPPQKVTPESSALSASSSPYALHRATIRSLTLPTKPNLDIPQSPLASPTPGANHKFSHFLELKKQGVHFNTKLASSSALKNPSLLPKLMDFAGVSGERQYTTTLPRELWNPAEFPTWAYKEELAEAQHGLSKKKGEERVKLQRESIDFVAADTPGQPSGLVVTKGKRASAAERVMAGLGRDRKPSPQLSTAGVRNEVEQRGRTSDGLHAGTRIVNRIRAPKELGVQRYQLGNTVTSARLDVKTMILTDAVGRRNLVPRAIPTREMAVPVGDSGGASPKVVTRPNPPYEAERVDMIGRSYQKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGR4</accession>
    <name>A0A8H6FGR4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="15">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_008726</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155591.1">
        <property type="nucleotide sequence ID" value="XM_037299590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGR4"/>
    <dbReference type="GeneID" id="59337121"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02083">
        <property type="entry name" value="P-type_ATPase_SERCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000005">
        <property type="entry name" value="Calcium-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000021">
        <property type="entry name" value="calcium-transporting ATPase, endoplasmic reticulum-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000037">
        <property type="entry name" value="Calcium-transporting ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000014">
        <property type="entry name" value="Calcium-transporting ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000065">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005782">
        <property type="entry name" value="P-type_ATPase_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01116">
        <property type="entry name" value="ATPase-IIA1_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 15" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="288"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="821"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="890"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="922"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="963"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="3"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6227283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="998" mass="108692" checksum="BC752D88E8F5468A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH79</accession>
    <name>A0A8H6FH79_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008202</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156406.1">
        <property type="nucleotide sequence ID" value="XM_037299070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH79"/>
    <dbReference type="GeneID" id="59336599"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021132">
        <property type="entry name" value="Ribosomal_eL18/eL18-A/B/_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01106">
        <property type="entry name" value="RIBOSOMAL_L18E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="2"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="162"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6228472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="191" mass="21671" checksum="F84DD86D9229CD48" modified="2022-01-19" version="1">MGIDLDRHHVRSTHRKAPKSDNVYLKLLVKLYRFLARKTDSNFNKVVLRRLFMSRTNRPPLSLSRLCMLASRGTREKIAPTEKTIVVIGTITDDTRLLKVPEKLTIAALHFTATARARIEKAGGECLTMDQLALRAPTGSNTLLLRGPKNAREAVKHFGFGPHKGKKPYVESKGRKFERARGRRRSRGFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHE0</accession>
    <name>A0A8H6FHE0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Vacuolar fusion protein MON1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007946</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">In complex with CCZ1, is required for multiple vacuole delivery pathways including the cytoplasm to vacuole transport (Cvt), autophagy, pexophagy and endocytosis. The MON1-CCZ1 complex acts at the fusion of vesicles with the vacuole, through its regulation of the SNARE complex during the coordinated priming and docking stages of fusion, and particularly at the stage of tethering/docking.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Required for multiple vacuole delivery pathways including the cytoplasm to vacuole transport (Cvt), autophagy, pexophagy and endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endosome</location>
            <location evidence="4">Multivesicular body membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 4">Prevacuolar compartment membrane</location>
            <topology evidence="1 4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Vacuole membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MON1/SAND family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156150.1">
        <property type="nucleotide sequence ID" value="XM_037298814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHE0"/>
    <dbReference type="GeneID" id="59336343"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004353">
        <property type="entry name" value="Mon1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027">
        <property type="entry name" value="SAND PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027:SF7">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN MON1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01546">
        <property type="entry name" value="YEAST73DUF"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 first Longin" evidence="6">
        <location>
            <begin position="255"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 second Longin" evidence="7">
        <location>
            <begin position="417"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 third Longin" evidence="8">
        <location>
            <begin position="565"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF19038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="675" mass="74394" checksum="21ABB96A24B4AACD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHN6</accession>
    <name>A0A8H6FHN6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dienelactone hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007988</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156192.1">
        <property type="nucleotide sequence ID" value="XM_037298856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHN6"/>
    <dbReference type="GeneID" id="59336385"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030638">
        <property type="term" value="P:polyketide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436:SF3">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436">
        <property type="entry name" value="POLYKETIDE CYCLASE SNOAL-LIKE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="39108" checksum="9949B152A3C7CA42" modified="2022-01-19" version="1">MRFSWSDGSAAHPKIVITAQDDDFDQVTIQNWRAEGFEVSYLPFTGLRNDYVHTLQHLADPLELGENFAIVAYGEAASEALDVAVKPMPKLCALIAYYPEKVPAPGAGFPPSHNVAIHFAGPQSKTPNCRCYSYPGVEAGFAEENLEQYDKVSASLAWSRSLAVLRKGFEVEVDLEKIWDDHVALEFVTKDADATMSTMVAQPYVNHIPTMTGGIGYKDLHRFYRDYFIPGNPPSLKMKLLSRTVGTDRVVDEMYMSFEHTQVMPWLLPDVEPTNKHVEVALVVIVCIRGGKLYHEHIYWDQASVLVQIGLLDPKLVPGGKGQGRLPVVDGAGARKVVDESSIRSNELISSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI59</accession>
    <name>A0A8H6FI59_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156608.1">
        <property type="nucleotide sequence ID" value="XM_037297973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI59"/>
    <dbReference type="GeneID" id="59335479"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="189" mass="21360" checksum="4A5C8A42959696CE" modified="2022-01-19" version="1">MSLTEVAPTQFELLRVILSAFHLPPGISALHMQSGIQVSLPKSEAGAPTQFKAIVSDCTNKNLLLKSFYDAKVRGKVFDRTKCVLSTLADNGISRCAFTSTFGMMQGQSWLNDQTAPQFKVDGLSRVPFMHQGYFFSPGFLICNHIFCRQEYETQFVAILSLDFLWEYFIRAQFHKKGWMIQLPPPPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ06</accession>
    <name>A0A8H6FJ06_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007499</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157025.1">
        <property type="nucleotide sequence ID" value="XM_037298390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ06"/>
    <dbReference type="GeneID" id="59335898"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF27">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G15730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6229383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="60624" checksum="6F5510514BA7126E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKU0</accession>
    <name>A0A8H6FKU0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004694</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157609.1">
        <property type="nucleotide sequence ID" value="XM_037295608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKU0"/>
    <dbReference type="GeneID" id="59333100"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034162305" evidence="2">
        <location>
            <begin position="17"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="467" mass="48702" checksum="22587DE26263B1F8" modified="2022-01-19" version="1">MLLPTVVLLLASFCNAEERSNPHETTLAPRAVNILIPTVTLAPVTNFNQSLPAGLFVNVTGPQIEFITDVPSLNGDALLALGPDAAAALEIAYQENCPNGLDSPNCETSLQLALNVDQPNLQKRVVVFLVPILVEGMAIEFAEMRKQENAISRIRTPSPNVAMLSSVQGRKTVVFATQTDGGKLITAVPSPTVDSTSQFSVATVTVGSSLAQKGDLIFAMPSEAGFALSQFVQTYGRCTVTRKRSCDPYETAGYVAGQAIAEVKPGGVTKDLITMPTDISFGDLIQPLAAPLPQVVLFGRQVTALAADSALLIIVAAFLLLAAYAELTTTAPGPTRSIIPASDFSTSRMPQRTGSECPQHVPNCSNCGGNSYIQPDPLHTNGNCVGIKTTKYNNFAAGCVCVDPIDPPGFQPYDDSEAISEAQAFLVAMAADGTGGAISTSGSTTISASSSPSASPAKACNNGVSYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FLN2</accession>
    <name>A0A8H6FLN2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157830.1">
        <property type="nucleotide sequence ID" value="XM_037290956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FLN2"/>
    <dbReference type="GeneID" id="59328435"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="286" mass="32758" checksum="74D944490806BFC7" modified="2022-01-19" version="1">MDEQRLEDFLATFPTSASLPKASPVPVSRYKWVLGILLVTIIAFPFAVYSTKGWSISESSTRPFPELAFLKHMQQGKSDLEAVGRLLETNIQFDPKDSRMSRAQYLKLLSTTSAAAVSAKFISFYPQAIDTVCTEYSWERIRKYLPWLVARFRMETKHMVRQHHATIDQAISVQEMLQEKSTEVDFHLIEHNETIMGKSYTGGAWRPRKEQQPLMQEISQKLNNGISSYRMSLDFWNRVGVSADRIYSNVSAMSMSHGHGARDRDVEMLLNDIKKALREAIEEIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY77</accession>
    <name>A0A8H6KY77_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Synaptobrevin homolog YKT6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_007134</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156662.1">
        <property type="nucleotide sequence ID" value="XM_037298027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY77"/>
    <dbReference type="GeneID" id="59335534"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000017">
        <property type="entry name" value="Synaptobrevin-like protein Sybl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="11">
        <location>
            <begin position="15"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="150"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6229020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="27646" checksum="478051006FE2499A" modified="2022-01-19" version="1">MASSSSSTPLLYSCIAYRTTILTEHTTSASSGTSSLASLILPKIDHSKPQKLTYTHNQNFIHYIADAPSDYPSSDPTAGGLTYLVVASSSFGRRIPFGYLVEIKKQFLARYDPDSTDFASLPPYGAADFNAQLKKLMVAYGTTEGGKQDAITNTQQEINDVKGIMTQNIERVLERGERIDLLVDKTDRLGVGARDFRVRSRGLKRRMWWKNVKLMVLLGVVVVFLLYLFIGFGCGLPGWSRCVGSKSKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYF9</accession>
    <name>A0A8H6KYF9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007225</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156753.1">
        <property type="nucleotide sequence ID" value="XM_037298118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYF9"/>
    <dbReference type="GeneID" id="59335625"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="155"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="212"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="281" mass="32524" checksum="46F2295BF412667C" modified="2022-01-19" version="1">MDSTSKEPASAPYESDPSGETEGNPSQNPSLPVPHDILVVEDAGKPYNPWPAADKELVMSLRNDGMSFRDISKRFTDRSARACRLLYDRVLETMQKLREEWPWTSSEEQLLFSLHKAGYPPRKVGDEFPERTGLAVKVEWEYMDAPVDYEDDYQWTELEDQQLQDFHKAGNDWMSIARLMPGLRRTAGDCLVRYLQTHGSLPYPCPTASAFPWAPEEIASLIRMRDQGRDWVEIASQLPDHDAVDCRNYWYNNHWDDYREWFKDGQIDMSRYAKSSKNTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYU7</accession>
    <name>A0A8H6KYU7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007479</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157005.1">
        <property type="nucleotide sequence ID" value="XM_037298370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYU7"/>
    <dbReference type="GeneID" id="59335878"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021842">
        <property type="entry name" value="DUF3435"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37535">
        <property type="entry name" value="FLUG DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37535:SF3">
        <property type="entry name" value="FLUG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11917">
        <property type="entry name" value="DUF3435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="136" mass="15444" checksum="0EB6BCC87CDBA119" modified="2022-01-19" version="1">MNRLLNSLTETAGYEKGSIAIHTLRRGFDNRVQPHVTTTELSQALGHKSQKMYEQKYKSRISSIDGQALFLRGTTNAKSIEYLRSAARHRNADLPRKLPAQVFADLEDSQEMTALRGRLVALSSEEWDERKKIGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZM1</accession>
    <name>A0A8H6KZM1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prefoldin subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004395</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157313.1">
        <property type="nucleotide sequence ID" value="XM_037295312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZM1"/>
    <dbReference type="GeneID" id="59332803"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23163">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000002">
        <property type="entry name" value="Prefoldin subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13723" checksum="7FD08E09E1DF9737" modified="2022-01-19" version="1">MAQSQITVRKQQELQQQYTNYKNSLQQLAQKIGDIEQDTEEHKLVLETLAPLPPDRKCFRMINGVLVEKTVKDVTPALQTNSEGLKKVLEELVKQYKRQQDDMEKWKKKNNIQVVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5Y5</accession>
    <name>A0A8H6C5Y5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147113.1">
        <property type="nucleotide sequence ID" value="XM_037297677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5Y5"/>
    <dbReference type="GeneID" id="59335181"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="237" mass="26466" checksum="3CA8724200A85831" modified="2022-01-19" version="1">MAPAPPKCEKAVTRYDNKITPKPAVKYEPTSTPTPKPKGKAARKSKTTVGLESRPKLGCVTPELTKALNHMARGFTPISDRKLIEEFFRYHRYQLRSNTDDESRADNDGRVQVASEELNNVVDTSKWFRETAEIPPQAQALLEQYSGLVPEQVIPHVTDLTHLTGALKGGERRGLSNDTAFFLHGLESFDKFWQDLGAASGSKLRVDARAEKATEATLSLPWSTPDDKMLVFTVRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6L2</accession>
    <name>A0A8H6C6L2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006688</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147021.1">
        <property type="nucleotide sequence ID" value="XM_037297585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6L2"/>
    <dbReference type="GeneID" id="59335089"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF61">
        <property type="entry name" value="CAPSULE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="2">
        <location>
            <begin position="548"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="864" mass="96594" checksum="2AAF7E6BF25164E2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6M1</accession>
    <name>A0A8H6C6M1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_006703</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147036.1">
        <property type="nucleotide sequence ID" value="XM_037297600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6M1"/>
    <dbReference type="GeneID" id="59335104"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1230">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF169">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="468"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="468"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="609"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="680"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6217601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="726" mass="79136" checksum="6C46736F64F016DB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6R5</accession>
    <name>A0A8H6C6R5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006405</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147428.1">
        <property type="nucleotide sequence ID" value="XM_037297303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6R5"/>
    <dbReference type="GeneID" id="59334806"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="574" mass="64929" checksum="A3690E52C5E6F1C1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6U1</accession>
    <name>A0A8H6C6U1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006467</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147490.1">
        <property type="nucleotide sequence ID" value="XM_037297365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6U1"/>
    <dbReference type="GeneID" id="59334868"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="175" mass="19001" checksum="DD6A4543D83EDAEC" modified="2022-01-19" version="1">MAAAIHGATQVVYRLDVHPKKKRPVEIYESAFLQSKLIRIQTTPRCFKSLLLPKQVQPETRSEIGRTGSETYIPPAKKMDRNIIGALVECKTIQGENSQHEDTSEGLCPAPTKIALETAPPTIGPTIGPPTTANAYPTIAVPRCVGSQMSPSTPPVFVTGADPKRPAKNRVMMMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C703</accession>
    <name>A0A8H6C703_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha-1,3-mannosyltransferase CMT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147216.1">
        <property type="nucleotide sequence ID" value="XM_037297506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C703"/>
    <dbReference type="GeneID" id="59335009"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021047">
        <property type="entry name" value="Mannosyltransferase_CMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34144:SF5">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE CMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34144">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11735">
        <property type="entry name" value="CAP59_mtransfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="521" mass="58983" checksum="C82E56C064A611AE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7E1</accession>
    <name>A0A8H6C7E1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147588.1">
        <property type="nucleotide sequence ID" value="XM_037297014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7E1"/>
    <dbReference type="GeneID" id="59334516"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="142" mass="15026" checksum="DBCA2044FDE4DBA4" modified="2022-01-19" version="1">MRSEGEECAAEDAGYTAEDEPGPAMRGVADVVTSGEGAGNGDKTGGSVETSGVRVGEADDRKYDAPALQSLLPLIFDLLKRIRQHTTDDLPQPESCKPDAEAWRLFGLGVPSAAAERRRGHDGGFKDAEEDALAESDGEEIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C836</accession>
    <name>A0A8H6C836_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_006053</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic component of the NuA4 histone acetyltransferase (HAT) complex which is involved in epigenetic transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4, H3, H2B, H2A and H2A variant H2A.Z. Acetylates histone H4 to form H4K5ac, H4K8ac, H4K12ac and H4K16ac, histone H3 to form H3K14ac, and histone H2A to form H2AK4ac and H2AK7ac. The NuA4 complex is involved in the DNA damage response and is required for chromosome segregation. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) through homologous recombination. Recruitment to promoters depends on H3K4me. Also acetylates non-histone proteins. In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as 2-hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA) or (2E)-butenoyl-CoA (crotonyl-CoA), and is able to mediate protein 2-hydroxyisobutyrylation and crotonylation, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148137.1">
        <property type="nucleotide sequence ID" value="XM_037296956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C836"/>
    <dbReference type="GeneID" id="59334458"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046972">
        <property type="term" value="F:histone H4K16 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000067">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF219">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="15">
        <location>
            <begin position="24"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6218702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="321" mass="36445" checksum="4BE04A73516E0615" modified="2022-01-19" version="1">MGSLSVSTNALASPLATHRNKEKPADLNVRNVVLGDILFKTWYPSFYPEELVGRELERLFVCPWCFKYSKDLMPYLAHTKVCPLKHEGPPGRPIYSRDLHAIYEVDGEEHQLFAQNLSLFAKLFLDNKSIFFDVTSFNYYLLVHTPNSTSSPHLQVVGFFSKEKMSWDNNNLACILVFPPWQRKGLGKILMGVSYELSRRENRIGGPEKPLSELGRRGYMRFWEARVARAILELKAKRTLSVGKIAEVCWVLPEDVVATLKDMGVLTASKRADGAVVFNKARLRDYVSKRGLDLTPPVSEEGFLDEWMPGPEESEVSISKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8R7</accession>
    <name>A0A8H6C8R7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004913</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148523.1">
        <property type="nucleotide sequence ID" value="XM_037295825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8R7"/>
    <dbReference type="GeneID" id="59333319"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF243">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA-2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="25"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="92"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="147" mass="17043" checksum="FF98685C38151452" modified="2022-01-19" version="1">MDRKQFVSQLLRRENSVQVEEGQECLICKEEYGARSSETETAERQIRLPCNPKHTVGSKCITAWLQAHNTCPVCRYEFFPAERSDKERAERLYIGFADNDDSSDEGEDTDDDYFSDEGGKTDDKDENMSDEDSEQDDEYDKDDNEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Z5</accession>
    <name>A0A8H6C8Z5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KANL3/Tex30 alpha/beta hydrolase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005359</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148251.1">
        <property type="nucleotide sequence ID" value="XM_037296269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8Z5"/>
    <dbReference type="GeneID" id="59333765"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046879">
        <property type="entry name" value="KANL3/Tex30_Abhydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026555">
        <property type="entry name" value="NSL3/Tex30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13136">
        <property type="entry name" value="TESTIS DEVELOPMENT PROTEIN PRTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13136:SF11">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20408">
        <property type="entry name" value="Abhydrolase_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KANL3/Tex30 alpha/beta hydrolase-like" evidence="2">
        <location>
            <begin position="41"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="459"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="674" mass="74460" checksum="C213533142D3ECD0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C936</accession>
    <name>A0A8H6C936_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148580.1">
        <property type="nucleotide sequence ID" value="XM_037295882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C936"/>
    <dbReference type="GeneID" id="59333376"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="232" mass="26306" checksum="5985881062D96CB2" modified="2022-01-19" version="1">MEMRGIDAHVSLIDSRRRKSFAYSDPKSSGSNGANQKEDDCSSMTSNVVGLHEPRLALANQPRPTCQEKFDMVWNDEVQDFVRMDAMRIGTHESYRSEVNKEGYHFRLPSSFPAFNTEDGADVTQVRPASTLAPDHTEGHTMSFEKWENPVVGTKVVFHKCDQSWRIPDMSSRRTRISQTPSPSKKRKLLCDDNTKFEPEQETPKANGLLVRRELLDQKITAVPRSPAAIHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9I9</accession>
    <name>A0A8H6C9I9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_005213</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148822.1">
        <property type="nucleotide sequence ID" value="XM_037296124.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333619"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20356">
        <property type="entry name" value="Rcat_RBR_HHARI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16625">
        <property type="entry name" value="RING-HC_RBR_HEL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000007">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000019">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048962">
        <property type="entry name" value="ARIH1-like_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007720">
        <property type="entry name" value="PigQ/GPI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329:SF3">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05024">
        <property type="entry name" value="Gpi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21235">
        <property type="entry name" value="UBA_ARI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1078"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1135"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1167"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1208"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1245"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1275"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="317"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="321"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="107"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="168"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6219387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1436" mass="162367" checksum="F4E81D045BE094D3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9N5</accession>
    <name>A0A8H6C9N5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004971</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148581.1">
        <property type="nucleotide sequence ID" value="XM_037295883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9N5"/>
    <dbReference type="GeneID" id="59333377"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052427">
        <property type="entry name" value="Glycosyltrans_GT2/GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47844:SF1">
        <property type="entry name" value="EXOSTOSIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47844">
        <property type="entry name" value="SYNTHASE CPS1, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G02500)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="373"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="402" mass="45897" checksum="B9D4323A2AF7BDC3" modified="2022-01-19" version="1">MTSTRALPDWLGCTPHPTELELLDHTLIFDVIHAVAHTRLLTVVLPGRNRLRLFENDSKPNLESSSDNSKRRSAALADSNDESDLFVPSLRQGTTLKKQRLESSAPKQRAVLRAKVALNSDNKARPEPRGQPEVWAEMRRALCESLTCYQAYQSGAYTWGSSNGSAGYAYGFLLDNDNDDYGYMDEKVVITRLGGWVKSTWKGLRARGRTSAHRTEILQPQSFRTGFLNEYILFGLIGPLNADDDNFITRWMVEHGWKLKIQYSEDACIETTLGEYPKFLSQCMRWARTTWRSNPLSLLSSRTWTTQPWSIYAVYLTSFVNFALFYDAVLLSTPWIYLNTQRISRSVEPFTGILTLCFWIFCTKLIKPRPHFRRHPRDLASSSPNTLPTPASNPYRISTTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAG0</accession>
    <name>A0A8H6CAG0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Plasma membrane ATPase</fullName>
            <ecNumber evidence="10">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_003727</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149337.1">
        <property type="nucleotide sequence ID" value="XM_037294649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAG0"/>
    <dbReference type="GeneID" id="59332138"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000008">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="362"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="732"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="759"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="798"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="869"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="907"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="12">
        <location>
            <begin position="110"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6219902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="970" mass="105263" checksum="06043F945C650848" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAK6</accession>
    <name>A0A8H6CAK6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149076.1">
        <property type="nucleotide sequence ID" value="XM_037295029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAK6"/>
    <dbReference type="GeneID" id="59332519"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="352" mass="39951" checksum="F4776FD9C38CDE86" modified="2022-01-19" version="1">MADCGYGDRRHNSETVPHGESRDAPAVPAKHQRDVKTERVGLLSLSRELRDKIYDHLLRIERWDVLKYKRDLFKVDLSILRVNQQLHQEASEIFYEGNTWVCITIEPSLLEALCNEWVAGRKGFPGDKPVSLATYTRTAPVAAATVTLQRLPQSGPGINLIHLLVSSFAVPRLCRILTVYANVRGLDIRVHLNALTAGKVKRAWHESLLDSFKDARGFGRATISDAQGNSPHVELATLMMSPFKHFQEIIDRASEHHDRARQKEELGRLSEARYGYQGGYDFTLWFDRSTHQLGFSTRIQYDGELGRSYIQLRTKLAFSCAFLCIKLGDLDLAYPIIERMLSLPPIDEHNVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBG8</accession>
    <name>A0A8H6CBG8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxisomal trans-2-enoyl-CoA reductase</fullName>
            <ecNumber evidence="13">1.3.1.38</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_003332</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in chain elongation of fatty acids. Catalyzes the reduction of trans-2-enoyl-CoAs of varying chain lengths from 6:1 to 16:1, having maximum activity with 10:1 CoA. Has no 2,4-dienoyl-CoA reductase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2E)-decenoyl-CoA + NADPH + H(+) = decanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44960"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:44961"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2E)-dodecenoyl-CoA + NADPH + H(+) = dodecanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:44965"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2E)-hexenoyl-CoA + NADPH + H(+) = hexanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44956"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:44957"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(2E)-octenoyl-CoA + NADPH + H(+) = octanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:44953"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2E)-tetradecenoyl-CoA + NADPH + H(+) = tetradecanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44968"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:44969"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a (2E)-enoyl-CoA + NADPH + H(+) = a 2,3-saturated acyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:33763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.1.38"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:33764"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with PEX5, probably required to target it into peroxisomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.38" evidence="13"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149636.1">
        <property type="nucleotide sequence ID" value="XM_037294255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBG8"/>
    <dbReference type="GeneID" id="59331743"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019166">
        <property type="term" value="F:trans-2-enoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033306">
        <property type="term" value="P:phytol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052388">
        <property type="entry name" value="Peroxisomal_t2-enoyl-CoA_red"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24317">
        <property type="entry name" value="PEROXISOMAL TRANS-2-ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24317:SF7">
        <property type="entry name" value="PEROXISOMAL TRANS-2-ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6220201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="301" mass="31419" checksum="6D4ACBD1473E0F8C" modified="2022-01-19" version="1">MPDLNDSSHFSPYRSDGKLYGFVCVVTGATQSIGQAIVGELAAHGAACIYACTRTTNEDTSDIASTLSETHPNTKIIPYPLIAADEQATLTLIDDILNSWGRLDVWVSSSGLLGPPSIDMTTPSDLQKCFEANSLAPFFALKYAPAAMGKLASKGNYPNAAPKDQKYGSIIVVSSVASTYGGCWGPCYTMSSHAALGVVQAGVAVLKGSGVRINCISPGQIDVGIDLKGFDMRGMKSQLPPANLQSPESQKEHIGLERAGSPSEVARVAGFLASGFSSYMTGANMVVDGGASVMMPLTLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBH4</accession>
    <name>A0A8H6CBH4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEBP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149586.1">
        <property type="nucleotide sequence ID" value="XM_037294205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBH4"/>
    <dbReference type="GeneID" id="59331693"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030162">
        <property type="term" value="P:regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF148">
        <property type="entry name" value="CARBOXYPEPTIDASE Y INHIBITOR"/>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034601363" description="PEBP-like protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="248"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="27856" checksum="C4C7E9C84E116492" modified="2022-01-19" version="1">MRCVTFPFALFVWIVFTKLCGAWTPLQNAFNDRPATASESVHEALVKAAIIPDILDDFQPSVYVTLSYASRHKTVDLGNKIKPKKVTFPPVFNIDASAMAPTRTLRSNTTYTLVLSDPDATSRAEPVMAQMCHWIVTNLTLPFQSPYSDGALDWLSSFFYTKEPEVFSVQEIMPYLPPTPPPETGYHRYVFILLAPATENDIQGELTKPKDRPHWGYGKVGAGVREWADENNLVAVGANFFYSKDKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBK9</accession>
    <name>A0A8H6CBK9_9LECA</name>
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            <fullName evidence="2">GYF domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ORF">HO133_002935</name>
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    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
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            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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            <end position="845"/>
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            <begin position="1"/>
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            <begin position="577"/>
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        <location>
            <begin position="1152"/>
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            <begin position="1177"/>
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        </location>
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        <location>
            <begin position="1514"/>
            <end position="1530"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1566" mass="166032" checksum="6D2E0538B635BABB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBP1</accession>
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        <recommendedName>
            <fullName evidence="7">U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase</fullName>
        </recommendedName>
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        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037293839.1"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:methyltransferase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="METTL16/RlmF"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05971">
        <property type="entry name" value="Methyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
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    <keyword evidence="2" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        </ligand>
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        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        </ligand>
    </feature>
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        <location>
            <position position="130"/>
        </location>
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            <name>S-adenosyl-L-methionine</name>
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        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        </ligand>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR037350-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="48303" checksum="718E79140C54A629" modified="2022-01-19" version="1">MMAKRNIYEEDVDFAVLALQSPAFNKYLKSNGQLDFSNPDAVQQLTKSLLERDFGIKLDLPDDRLCPPVPNRFNYILWIQDLLDTTSNRYSDRYDAERTVTGLDIGTGASCIYPLLGCSQRPKWRFAVTDIDEKNMTYAKRNILQNNLKSRIRPLQTQPNDPLVPLDAFGLRSIDFTICNPPFYSSTSDLLSSAAAKSRPPCSACTGADVEMVTPGGEVAFVSRMIDESKVLKDRCQWYTSMLGKYSSVEIVVGNLRGAGVDNWAVKDLVQGNKTRRWAVAWSWGSMRPSHDAARGTSTLPKHLLPFPSESSFTVETSIESCSHRLNATLQALELRWQYRPIMATGVGFAKGSVWSRAARRKQQQSPTDSVEKDQAMDQDDENGEPALGFKIQLRKENQGGVGVMVRWLQGRDSVLFESFCGMLKRQLKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBQ9</accession>
    <name>A0A8H6CBQ9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002897</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
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            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037293822.1"/>
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    <dbReference type="GeneID" id="59331309"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034706177" evidence="2">
        <location>
            <begin position="20"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="364" mass="38345" checksum="2303827263E6A8E1" modified="2022-01-19" version="1">MANLLSYSLVAVLVAVSLAAPNPAVNRIDAPPKRPHSTTIIGSKTTLSSSTAPHITLKPRKPAVNRIDAVGVNVHGKPTSSSAHSSSFRTLVSTTVGPHIALNPRNPAVNKIDAPGHKPHSSTRTSSATAASSSNRPHAPIEERAPAVNRIDAVGNGVVDQALVSVVGGTPVTLAITDIQQLEVAATEIVGAAAAPMPVISVNPLPDNTAVPASFFAGPTPSSSAIPFEVPDTETVIYITVHPDRPIAKLDMGQVLLHAMSELRETINENGEDGWLPEDDWSWFAETWDCLLTADRNLIPGPGGRAQHLTYGVLHNALVGLWGAMFSHGRYFACDFEIEDAQWGIVGSGSMNSWDKSEGNLTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8H6CBS9_9LECA</name>
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        <recommendedName>
            <fullName evidence="12">RNA-binding protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="10" type="ORF">HO133_003083</name>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
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        </lineage>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May be involved in the turnover of nuclear polyadenylated (pA+) RNA.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220650.1"/>
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        <property type="nucleotide sequence ID" value="XM_037294007.1"/>
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="PWI_dom"/>
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        <property type="entry name" value="PWI_dom_sf"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14398">
        <property type="entry name" value="RNA RECOGNITION RRM/RNP DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14398:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN SWM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
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            <begin position="250"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
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            <begin position="354"/>
            <end position="426"/>
        </location>
    </feature>
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            <begin position="250"/>
            <end position="278"/>
        </location>
    </feature>
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            <begin position="114"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="283"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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            <end position="454"/>
        </location>
    </feature>
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        <location>
            <begin position="695"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="455"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="733"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00043866"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="743" mass="81944" checksum="FA625D8B700C452E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBX0</accession>
    <name>A0A8H6CBX0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Atos-like conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150022.1">
        <property type="nucleotide sequence ID" value="XM_037293944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBX0"/>
    <dbReference type="GeneID" id="59331432"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033473">
        <property type="entry name" value="Atos-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025261">
        <property type="entry name" value="Atos-like_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051506">
        <property type="entry name" value="ATOS_Transcription_Regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13199">
        <property type="entry name" value="GH03947P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13199:SF11">
        <property type="entry name" value="PROTEIN ATOSSA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chromosome_seg"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF4210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01177">
        <property type="entry name" value="DUF4210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atos-like conserved" evidence="2">
        <location>
            <begin position="364"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="744"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="806" mass="88717" checksum="72B73E1C958086B6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC60</accession>
    <name>A0A8H6CC60_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150140.1">
        <property type="nucleotide sequence ID" value="XM_037294062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC60"/>
    <dbReference type="GeneID" id="59331550"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="2">
        <location>
            <begin position="313"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="543" mass="62400" checksum="61ED95DF508DF893" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC70</accession>
    <name>A0A8H6CC70_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003002</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150004.1">
        <property type="nucleotide sequence ID" value="XM_037293926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC70"/>
    <dbReference type="GeneID" id="59331414"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF92">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="32"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="358" mass="40941" checksum="F3C5C1F3ABBE0D85" modified="2022-01-19" version="1">MSPFDDTGRIHKWPLLISNSVLFGVCLASVSVRYYVRFFIQKQLSIDDGILLFGILCLISAMVLLIIFVDDLFLVEALEGSTIPADLPVDFLQRVFDFEKLVVGGLVLTWCAIISVKFSYLFLFRKLIDRIRPMVLYWWVAVIFNALISIYGIIIYAVIRPWYCTTISVECLQGNGLDKSFAFAISQMVLDIFGDLLILVIPFHLIWRIKVRLSQKLVLASTLCFTVLTIMCTITRVVGVRTSSSDSSLDTVWQTYWQYITANIALTMTAATAFRAFFISRHQDRPAQRQGSNKSWISRSWRFFKLVLRPWSWQGKSSALQIGEDSSNRGIELPLIEERATMTGMRTFINRQGEFGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDF4</accession>
    <name>A0A8H6CDF4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150938.1">
        <property type="nucleotide sequence ID" value="XM_037293285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDF4"/>
    <dbReference type="GeneID" id="59330772"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="262" mass="28582" checksum="E1690DAF68D68B14" modified="2022-01-19" version="1">MLTRHILGAAARRHIQKGSLRGSIECQSQPSTETPTELGFIYREREKAGTTGRDTATIEKNKDVQGSVPAAGLSAHLTISQDQTSGSQLSQEKLAALSKPLDEADIAPGHRKAAGLDTIAARPTRSNSPGLRDPSRRRRRRSPEIVCIPANRSSDSSSIRKELIGSAVSISNDNVEEKSHLETWVSEITFNHPHRNSRGTLQESSRANNLNISTHASSPEQNYSMEDLLSRLKSLERENHEVRNGTAEETSHTAPPGEPSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDV9</accession>
    <name>A0A8H6CDV9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutaminase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001575</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151044.1">
        <property type="nucleotide sequence ID" value="XM_037292505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDV9"/>
    <dbReference type="GeneID" id="59329991"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052743">
        <property type="entry name" value="Glutaminase_GtaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032514">
        <property type="entry name" value="GtaA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033433">
        <property type="entry name" value="GtaA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987">
        <property type="entry name" value="GLUTAMINASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987:SF14">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_6G09910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17168">
        <property type="entry name" value="DUF5127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16335">
        <property type="entry name" value="GtaA_6_Hairpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034621406" description="Glutaminase" evidence="2">
        <location>
            <begin position="25"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminase A N-terminal" evidence="4">
        <location>
            <begin position="119"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminase A central" evidence="3">
        <location>
            <begin position="342"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="704"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="769"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="940" mass="101060" checksum="03041AA2BCA78C7E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE60</accession>
    <name>A0A8H6CE60_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="14">ATP-PFK</shortName>
            <shortName evidence="14">Phosphofructokinase</shortName>
            <ecNumber evidence="14">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_001592</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14 15">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14 15">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="14"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151061.1">
        <property type="nucleotide sequence ID" value="XM_037292522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE60"/>
    <dbReference type="GeneID" id="59330008"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000007">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000008">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002872">
        <property type="entry name" value="PRK03202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 14 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="14"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="402"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="14">
        <location>
            <begin position="1"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interdomain linker" evidence="14">
        <location>
            <begin position="388"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="14">
        <location>
            <begin position="402"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="85"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="115"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="161"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="205"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="295"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="536"/>
            <end position="540"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="574"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="581"/>
            <end position="583"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="641"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="667"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="673"/>
            <end position="676"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="745"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6221626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="779" mass="85034" checksum="4DD3FE1157E8F30D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEN8</accession>
    <name>A0A8H6CEN8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acetoacetyl-CoA synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001249</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151598.1">
        <property type="nucleotide sequence ID" value="XM_037292179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEN8"/>
    <dbReference type="GeneID" id="59329665"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030729">
        <property type="term" value="F:acetoacetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005914">
        <property type="entry name" value="Acac_CoA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01217">
        <property type="entry name" value="ac_ac_CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002937">
        <property type="entry name" value="PRK03584.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42921">
        <property type="entry name" value="ACETOACETYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42921:SF1">
        <property type="entry name" value="ACETOACETYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="6">
        <location>
            <begin position="50"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="121"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="687" mass="76588" checksum="DC90998EE6423F62" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEV6</accession>
    <name>A0A8H6CEV6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Heme oxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151520.1">
        <property type="nucleotide sequence ID" value="XM_037292101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEV6"/>
    <dbReference type="GeneID" id="59329587"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004392">
        <property type="term" value="F:heme oxygenase (decyclizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006788">
        <property type="term" value="P:heme oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19165">
        <property type="entry name" value="HemeO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002051">
        <property type="entry name" value="Haem_Oase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10720">
        <property type="entry name" value="HEME OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10720:SF0">
        <property type="entry name" value="HEME OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="29510" checksum="061C427A45E1F2D0" modified="2022-01-19" version="1">MAHLLPIYEAFEGPFRSLVGAGGDDEGGGEEAAEEVVKGLRGMYARGLERGARLTGDVETVLRGRGVDPGRRPRLEAFTERIRAAVPGRPHLLLAYTWVLYLALFSGGRYIRAGLRGAGAGFWHRGVEGGSEGGFLGTGLAGEGKGGGVDGCLGFWTFEGEADGEDLKAGFKARFAAVEGTLTEVEKEEVVREAVFIMQSMTGVVVEISEVVGTGNPVAAGQWVDQRLDGRKDEDEPSMRWLLLKHVLPMGMVELIAAGARSAISVGMVPSFWSAGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF87</accession>
    <name>A0A8H6CF87_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001379</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151728.1">
        <property type="nucleotide sequence ID" value="XM_037292309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF87"/>
    <dbReference type="GeneID" id="59329795"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056236">
        <property type="entry name" value="HMA_PCA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01511">
        <property type="entry name" value="ATPase-IB1_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594:SF4">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24534">
        <property type="entry name" value="HMA_PCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="517"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="587"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="737"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="775"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1088"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1118"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="9">
        <location>
            <begin position="291"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="157"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6222293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1143" mass="121390" checksum="B382E46D5B5B1BA2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFD9</accession>
    <name>A0A8H6CFD9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SMODS and SLOG-associating 2TM effector domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001307</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151656.1">
        <property type="nucleotide sequence ID" value="XM_037292237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFD9"/>
    <dbReference type="GeneID" id="59329723"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041622">
        <property type="entry name" value="SLATT_fungi"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033635">
        <property type="entry name" value="SLATT_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38793">
        <property type="entry name" value="SLATT_FUNGAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38793:SF3">
        <property type="entry name" value="SMODS AND SLOG-ASSOCIATING 2TM EFFECTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18142">
        <property type="entry name" value="SLATT_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="SMODS and SLOG-associating 2TM effector" evidence="3">
        <location>
            <begin position="62"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="321" mass="35204" checksum="B211C3EE3DD8E7B1" modified="2022-01-19" version="1">MGETEPELTYHKPTPTFDRDLEKAGSTDRLSVFREQVGISDVIELSPRASRRPAPNIGIYNRVSTEEHKAKLQYYSSALVINACLLTQVIFASALTALGAGGGSHIQITVLGAANTVIAAILTFTKGSGLPNRLRQYQCTLRKVREYIEQRERDFAQLDCQLDLDHEMKIIKGMYEQARQNDENNDPGTYHNPTAASTANGPLTGKTLVSEPSSILGPDIRQPAPRVASAVAPQVPPTNQPKTEEDLTHNEPTPLGGVDRSATDRYKIAYSVADRLSATLIVCLLNSYQGVFILLDPGSLAPLFQHLRLKARYDANGNYQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH28</accession>
    <name>A0A8H6CH28_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000221</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152596.1">
        <property type="nucleotide sequence ID" value="XM_037291161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH28"/>
    <dbReference type="GeneID" id="59328640"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010961">
        <property type="term" value="P:intracellular magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12829">
        <property type="entry name" value="Alr1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000006">
        <property type="entry name" value="CorA family metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000008">
        <property type="entry name" value="CorA family metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044089">
        <property type="entry name" value="Alr1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535">
        <property type="entry name" value="MAGNESIUM AND COBALT TRANSPORT PROTEIN/MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535:SF55">
        <property type="entry name" value="MAGNESIUM TRANSPORTER ALR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="614"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="645"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="291"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="672" mass="74919" checksum="6FE60565B156E245" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH80</accession>
    <name>A0A8H6CH80_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="7">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_000300</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Cleaves guanosine and inosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19805"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152674.1">
        <property type="nucleotide sequence ID" value="XM_037291239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH80"/>
    <dbReference type="GeneID" id="59328719"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000004">
        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006054">
        <property type="entry name" value="PRK08202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF9">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="9">
        <location>
            <begin position="29"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000477-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6223457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="314" mass="34281" checksum="53EDDD9F7673E581" modified="2022-01-19" version="1">MDSATSQQTAEALRYLQVKVPPLLHQPVIGIICGSGLKGLADVVLPEPRFEIPYADIPHFPQSTVHGHASKLLFGILELGALPVVLMVGRVHFYEGHSIQSITFPIRLMKRLGVDTIIVTNAAGGLNEDYGVGDLVVLNDHINLAGLAGIHPLRGPNADDFGVRFPALSDAYDLDLRRRAHHLWKELNRDAGSTRRLQEGTYAFVGGPSYETRAECRMLRSLGADVVGMSTVPEVIVARHCDMKVLAISLVTNKALLEAGPRGDNVHIQGSSRQYLREAIEKGKANHDEVLDAGEKAAREVQVRRRSFNQGVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH97</accession>
    <name>A0A8H6CH97_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PLC-like phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152411.1">
        <property type="nucleotide sequence ID" value="XM_037290976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH97"/>
    <dbReference type="GeneID" id="59328455"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF146">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26146">
        <property type="entry name" value="PI-PLC_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="37385" checksum="DE9B1179EB12674E" modified="2022-01-19" version="1">MRIRNTEVRLRVIFNYTILCLACTTILAIAIINFMEEITPMLVHLHTQAQVGCNGHVELCDRRYSNITQIATHDSAFVGILPTDDQDVDVTAQLDAGVRFLQAQTHRNVVGTLSLCHTSCFLKDAGSVESYLEVVKGWLDTHPKEVVTLLLTNGDNVPINDFDKVFTASGIQPYAYVPGPSHDPSILDSWPTLGELIGNGTRLVAFLDYGALDTVPYILPEFLYFWETPFDMTDPSFPQCIIDRPGGVVNDPAQSHHLMYIVNHFLDTEMLGMDIPDRRDARKTNSATSGESINAQVRLCQQQHDRAPKGILVDFFDKGDVFGVQDALNGFENAKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHB3</accession>
    <name>A0A8H6CHB3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000345</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152719.1">
        <property type="nucleotide sequence ID" value="XM_037291284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHB3"/>
    <dbReference type="GeneID" id="59328764"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07591">
        <property type="entry name" value="BAR_Rvs161p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000014">
        <property type="entry name" value="Protein hob3, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037429">
        <property type="entry name" value="Rvs161/Hob3_BAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF3">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01251">
        <property type="entry name" value="AMPHIPHYSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAR" evidence="4">
        <location>
            <begin position="17"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="261" mass="29831" checksum="ECF10E5A0DD7A66C" modified="2022-01-19" version="1">MSWAGFKKVANRATTQVMMKTGHVEKTNDRDYEVEERRYRTMESAANRLQKEAKGYLDSLRAMTASQMRIAETIDAFYGDAGANDGVSRSYKQAVEDLDAETIKALDGPFRQTVLEPITRFCAYFPDINECIKKRNHKLLDYDATRAKVKKLVEKPDKDPTKLPRAERESDLAKTAYEALNEQLFSELPQLIDLRVPYLDPSFEALVKIQLRFCAEAYSRMAQVQQFLDADTREQYAQGHLDSRVEQVLGEIRELSIAGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHC5</accession>
    <name>A0A8H6CHC5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152775.1">
        <property type="nucleotide sequence ID" value="XM_037291340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHC5"/>
    <dbReference type="GeneID" id="59328820"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="678" mass="76214" checksum="3A1371C69E9ACA6B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHF5</accession>
    <name>A0A8H6CHF5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152852.1">
        <property type="nucleotide sequence ID" value="XM_037291417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHF5"/>
    <dbReference type="GeneID" id="59328897"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006771">
        <property type="entry name" value="CetA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36195:SF4">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G01990)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36195">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G01990)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04681">
        <property type="entry name" value="Bys1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034039226" evidence="1">
        <location>
            <begin position="25"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="200" mass="20711" checksum="8AECCCB9DC570D0D" modified="2022-01-19" version="1">MHFTTTITALGFVLSAAAVPASQSWIVDPAAATDAPAVPDSGNPVAADANVAAGTTGDANVRNYCDFPVYLYVCGQDPASCTPEYTLAAKTGTYSEVFSTPNNGRSIKMGTTSGEVAKPILQFEYTNTGSGQVAYDLSEVNGNPFGPYGFFLTSNNNACFHEECPAPGTHDVCPWVFTTPTNGLVSDCPIASSIGVTLCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHR4</accession>
    <name>A0A8H6CHR4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lysophospholipid acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000269</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152643.1">
        <property type="nucleotide sequence ID" value="XM_037291208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHR4"/>
    <dbReference type="GeneID" id="59328688"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="62716" checksum="EDA66F5E0D0248C2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHY5</accession>
    <name>A0A8H6CHY5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010494</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152980.1">
        <property type="nucleotide sequence ID" value="XM_037301346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHY5"/>
    <dbReference type="GeneID" id="59338884"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="550"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="42"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="332"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="577"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="577"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="596"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="616" mass="68099" checksum="BCF90A5F40720E62" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIM6</accession>
    <name>A0A8H6CIM6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010752</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153237.1">
        <property type="nucleotide sequence ID" value="XM_037301603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIM6"/>
    <dbReference type="GeneID" id="59339142"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14372">
        <property type="entry name" value="CUE_Cue5p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000064">
        <property type="entry name" value="Similar to CUE domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041807">
        <property type="entry name" value="Cue5/Don1_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16461">
        <property type="entry name" value="TOLL-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16461:SF5">
        <property type="entry name" value="TOLL-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="64"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="434" mass="47258" checksum="519DCE935519A1C2" modified="2022-01-19" version="1">MSDSEKPKPKAPPESPTTAKSLDFDDETAETISFPLKSPPSTEKPKPGAEEEFPPPKPPRPLAPEQQAENTLKEAFPSIDAAVVKAVLRASGGRVEPAFNALLGMSDPDAAEPAPPPQPPRPQRAPTGPTSTEESQLAADERYARQLAEHYNGAPAYGEGPRSGSGNRRPQQRGPPRQASGYDQDDGPSWFDEELPILKDNIKKGFLETQSTVNKWVNSFKKKIDGEGDDDFQGRPPQQGYPAQQQSDGRRSSEFGRRSADRERYDADPQVLGDDFAGLQMRDNEAQRRSSRPLANPDLFKPTPPRPQSTQSGRRVSFQEGPPEEIEIHYPKSPDPTNRPSSGTGGKSSKWQPLAPVDPSPVADHDPFSLGDSDDEEAKKKDVKADDSGRLQQAAAEAMSDDIGSSGEKKLEPQERSGSLKTKDKETETLVGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIP6</accession>
    <name>A0A8H6CIP6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SNF7 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010746</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153232.1">
        <property type="nucleotide sequence ID" value="XM_037301598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIP6"/>
    <dbReference type="GeneID" id="59339136"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="186"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24254" checksum="2F7E58D011535CC0" modified="2022-01-19" version="1">MGNTPSSHKISAQDRAILDMKNQRDKLHQYQQRVTLITDRETAVARECLARGDKPKALLALRRKKFQETLLAKTDAQLETLEQLTSNVEFTLVQKDVVYGLQQGTNVLKQIHAEMGGIENVEKLMGESEDAKAYQKEISEMLGGQMSNQDEDEVEDELETLEGEINGIKEPNAIKYPAAPQNEPVLAGELEEVGQQKARARARAQERAAAQAAEPMLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIX8</accession>
    <name>A0A8H6CIX8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Carboxypeptidase</fullName>
            <ecNumber evidence="15">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_010623</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153109.1">
        <property type="nucleotide sequence ID" value="XM_037301475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIX8"/>
    <dbReference type="GeneID" id="59339013"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000121">
        <property type="entry name" value="Carboxypeptidase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF190">
        <property type="entry name" value="PHEROMONE-PROCESSING CARBOXYPEPTIDASE KEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4 15" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034645772" description="Carboxypeptidase" evidence="15">
        <location>
            <begin position="36"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="478"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="577"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6224049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="627" mass="70603" checksum="4273D96751EFA797" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ83</accession>
    <name>A0A8H6CJ83_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cellulase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_011081</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153564.1">
        <property type="nucleotide sequence ID" value="XM_037301930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ83"/>
    <dbReference type="GeneID" id="59339471"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142:SF5">
        <property type="entry name" value="CBM1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="7">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="280" mass="30327" checksum="640F70F6FFC53463" modified="2022-01-19" version="1">MQHFVNDDKLNLFRLPVGWQYLVNHDLGGALDQTFFSTYDQIMQACLATGAHCILDVHNYARWNGGIIGQGGPSNDDFGNLWAQLAQKYASQGNVIFGLMNEPHDLDMTTWAASVQQAVYAIRDNGATTQMILLPGTNYDAVGGFQSNSAPALSSVQDYDGTHNKLIYEAHQYLDGGGGTATECDTNGVEYTLAPLTTYLRSVGRQGMLTETGGGNTASCYTDVCAELSHLNYNSDVWLGWVGWAAGSFDGSYNLTETPNGNQDTTLVSYCIAGKFDNPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJC3</accession>
    <name>A0A8H6CJC3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF1793-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010972</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153455.1">
        <property type="nucleotide sequence ID" value="XM_037301821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJC3"/>
    <dbReference type="GeneID" id="59339362"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032515">
        <property type="entry name" value="DUF4964"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052743">
        <property type="entry name" value="Glutaminase_GtaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032514">
        <property type="entry name" value="GtaA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033433">
        <property type="entry name" value="GtaA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987:SF1">
        <property type="entry name" value="GLUTAMINASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987">
        <property type="entry name" value="GLUTAMINASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16334">
        <property type="entry name" value="DUF4964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17168">
        <property type="entry name" value="DUF5127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16335">
        <property type="entry name" value="GtaA_6_Hairpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4964" evidence="2">
        <location>
            <begin position="206"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminase A N-terminal" evidence="4">
        <location>
            <begin position="290"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminase A central" evidence="3">
        <location>
            <begin position="599"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="981" mass="108659" checksum="890ED88BADF8CDCB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJQ0</accession>
    <name>A0A8H6CJQ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_011048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153531.1">
        <property type="nucleotide sequence ID" value="XM_037301897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJQ0"/>
    <dbReference type="GeneID" id="59339438"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000220">
        <property type="entry name" value="UDP-galactopyranose mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734:SF4">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="2">
        <location>
            <begin position="18"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="547" mass="60778" checksum="09FCF4494FF602F2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK63</accession>
    <name>A0A8H6CK63_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010212</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153884.1">
        <property type="nucleotide sequence ID" value="XM_037301067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK63"/>
    <dbReference type="GeneID" id="59338604"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007300">
        <property type="entry name" value="CidB/LrgB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249:SF0">
        <property type="entry name" value="PLASTIDAL GLYCOLATE_GLYCERATE TRANSLOCATOR 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249">
        <property type="entry name" value="PUTATIVE SEROTONIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04172">
        <property type="entry name" value="LrgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="623"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="657"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="687"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="341"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="357"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="715" mass="79380" checksum="FEA25017F5B8568E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKD2</accession>
    <name>A0A8H6CKD2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010361</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154031.1">
        <property type="nucleotide sequence ID" value="XM_037301214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKD2"/>
    <dbReference type="GeneID" id="59338752"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF31">
        <property type="entry name" value="MONODICTYPHENONE CLUSTER TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="418" mass="46420" checksum="06977BD9A8803612" modified="2022-01-19" version="1">MPANSHSIKVQKLRSSCDSCNLAKLKCSRSRPSCTRCESQGVKCVYGVSLRAGKHRANGSNVQKPSPESPLSAKVSTKESTPVPTFSTAEISRTLEPAFSSILDSMPMDTDWSSFLAEDNPYLLSNSMDLNNKDISPQEQSWKPSIAQSHSDLSLNTFQFDPRYTFAARPQISPQSSRCRTFSVSTLLSEAQKSPLFQSNDPCLCGRKILQQLLTLTATPDVPAAFDAALNQNKHIIALCHSILNHETHQHRDISFVLTLTALIAKVIAVYDTIYKSHHLTKGYCRRPAIKSRYFPDVGIGNGDSAESFEPNGINIEHHSSLPSPRSLTGMTKPSLAPVPVRLTMGAYQLDEEDEGRMILDILKLELSKVSALIKTFEHLFCGTGPDDQESKHESKAYEDMVYYLQKRLRVTLETPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKF5</accession>
    <name>A0A8H6CKF5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GABA permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010391</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154061.1">
        <property type="nucleotide sequence ID" value="XM_037301244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKF5"/>
    <dbReference type="GeneID" id="59338782"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF8">
        <property type="entry name" value="PERMEASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="57022" checksum="451C249438877784" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKH8</accession>
    <name>A0A8H6CKH8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010436</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154106.1">
        <property type="nucleotide sequence ID" value="XM_037301289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKH8"/>
    <dbReference type="GeneID" id="59338827"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF16">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="9"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="287" mass="30563" checksum="04FAD1BC77C26EDE" modified="2022-01-19" version="1">MPTKIVVCGGNGFLGSRICRVAVGRGWDVTSISRSGEPTWSAITSSPNAPPWSSTVKWQKADILKPETYKPMLNGADAVVHSMGILLEADYKGVVTGKESPWSGLSRAFSATKGGSQNPLERKEGEALEPQEKDGQLTYELMNRDSAITLAQEASSQNVPSFVFISAAAGAPMLPKRYITTKRDAESTIASSLPKLRSIFIRPGFLYDSSRMFTMPIAAAGSIGNMVNSMIGGRLSWIAGAAVEKPLKPDLVAEAVVEAIEDGAVKGVVGTSDIEALATKAWRRGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKI9</accession>
    <name>A0A8H6CKI9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153658.1">
        <property type="nucleotide sequence ID" value="XM_037300841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKI9"/>
    <dbReference type="GeneID" id="59338377"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="66" mass="6817" checksum="53514DA52654A8CA" modified="2022-01-19" version="1">MPSAQASSPPPPPFELRKTAPIRFQNSGDGGIGDFSSALGGDSDSDVESDRGGVDGLDDFFGDEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKV8</accession>
    <name>A0A8H6CKV8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cystathionine beta-lyase</fullName>
            <ecNumber evidence="3">4.4.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cysteine-S-conjugate beta-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_010166</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L,L-cystathionine + H2O = L-homocysteine + pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13965"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an S-substituted L-cysteine + H2O = a thiol + pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:18121"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29256"/>
            <dbReference type="ChEBI" id="CHEBI:58717"/>
            <dbReference type="EC" id="4.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.13" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153838.1">
        <property type="nucleotide sequence ID" value="XM_037301021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKV8"/>
    <dbReference type="GeneID" id="59338558"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000013">
        <property type="entry name" value="Cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000009">
        <property type="entry name" value="Cystathionine gamma-synthase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006238">
        <property type="entry name" value="Cys_b_lyase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01329">
        <property type="entry name" value="cysta_beta_ly_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF50">
        <property type="entry name" value="CYSTATHIONINE BETA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5 13 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6224971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="460" mass="49489" checksum="9234CAB6A26DB178" modified="2022-01-19" version="1">MSSFAPDPLGKDPAEESSKSSKSLSKAFSRVDLDGHDLPPSPAPSSPRNGKRYALATELVYTEGTDQYNASSTPIYQSATFKQTSAGGEGAEYDYTRSGNPTRTHLERHLAKIMCAERALVVSSGMGALDVITRLLRPGDEVVTGDDLYGGTNRLLKYLATNGGIIVHHVDTTKPTSVQRVVGPKTAMVLLETPTNPLIKIVDIPTIANLAHEANPEALVAVDNTMLSPLLQNPLELGADIVYESGTKYLSGHHDLMAGVLAVNSSAIGDKLYFTINASGCGLAPFDSWLLMRGIKTLKVRMEQQQSNAQRIAEFLESHGFKVRYPGLKSHPQYALHNSMARGAGAVLSFETGSIHVSERIVEAAKLWAISVSFGCVNSLISMPCRMSHASIDAKTRQERALPEDIIRLCVGIEDADDLIDDLSRALVLAGAVNLTPEGFHHAAEKGPTAKLDTQSNNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL94</accession>
    <name>A0A8H6CL94_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_010448</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154118.1">
        <property type="nucleotide sequence ID" value="XM_037301301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL94"/>
    <dbReference type="GeneID" id="59338839"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="525"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="120"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6225251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="623" mass="68516" checksum="E91B000EC358CF27" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLT0</accession>
    <name>A0A8H6CLT0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">VLRF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_009609</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The VLRF1 domain mediates binding to the 60S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ANKZF1/VMS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154318.1">
        <property type="nucleotide sequence ID" value="XM_037300470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLT0"/>
    <dbReference type="GeneID" id="59338004"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047139">
        <property type="entry name" value="ANKZ1/VMS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041175">
        <property type="entry name" value="VLRF1/Vms1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036">
        <property type="entry name" value="ANKYRIN REPEAT AND ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036:SF2">
        <property type="entry name" value="TRNA ENDONUCLEASE ANKZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18826">
        <property type="entry name" value="bVLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52044">
        <property type="entry name" value="VLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VLRF1" evidence="13">
        <location>
            <begin position="236"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="485"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="114"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="559"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="562"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="613"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6225609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="650" mass="70985" checksum="07BBF730EC7E5276" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMH2</accession>
    <name>A0A8H6CMH2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154918.1">
        <property type="nucleotide sequence ID" value="XM_037300092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMH2"/>
    <dbReference type="GeneID" id="59337626"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034182188" evidence="1">
        <location>
            <begin position="20"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="407" mass="43707" checksum="68F9694958864AA6" modified="2022-01-19" version="1">MYLFKCLAGLSAVLLGVAALPQPIPAHTPAALYDIVERAPEPTLEPRQGAGSQPKGFTQVPYGPGAPVWATDLDPAAPCADPTIPDASCWKALDLTGYINWWCVGLAFIPDLGALIKGIIIAAQQVPGVAKNIFPTGTTDSETFDFKKVTTSLGTLVTQFRTNYKAVLAEAENDLTSFLAFVENTPLSGEIPDLDSVVDQCLTSLYTYVISQAYQVSNVIITRQIDTDVNALSSNGTALNWDTGCGKGYGQYGECSTFWYDTATSITYALYDTQTQTKDFNTEYQNFFNGYSTGEKFFKGADLCAQASHSTQGSQAIVDTSPGGSTTYCLSNMQVCTWDLTEINTHPNPFTDCASNVAPKFYAQGCLGEIGDFGGGAAPVCYLGWGLLRNRNYKNSDHEFCVAYGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNT6</accession>
    <name>A0A8H6CNT6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155197.1">
        <property type="nucleotide sequence ID" value="XM_037299196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNT6"/>
    <dbReference type="GeneID" id="59336726"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="731"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="501"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="618"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="758"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="796"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="835" mass="92146" checksum="14473923E278DED3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNX9</accession>
    <name>A0A8H6CNX9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008465</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155332.1">
        <property type="nucleotide sequence ID" value="XM_037299331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNX9"/>
    <dbReference type="GeneID" id="59336861"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510:SF1">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="3">
        <location>
            <begin position="53"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="406" mass="45239" checksum="7C9B31BDBFCA3AE0" modified="2022-01-19" version="1">MTIAKVAPFAVEQWMDAHETHARYNIAETCVASISLDDLRGLSEDKSSEIWSSTTKLTYGSIRGSEKLRTNLANRYSAKKPFQADNILITPGAIAANMTVFYGLIGKGDHVICHYPTYQQLYEIPERLGAEVDYWRAEESKKWQLDIEDLKSLIRPNTRMIIINNPHNPSGAIVPKSTLDLLIEVAEAHDLIIMADEVYRPIFHSISPGSPDFPPSVLSLGYAKTIATGSLSKAYSLAGIRVGWIASRSTEIIEACAQARDYTTISVSQLDDQIAAYALDNDCIHSLLSRNIQLAKRNLEILERFVESHRWACEWVKPVAGTTAFIKISHMGTPVDDVAFCQKLMEDASVMLVPGSRCFGNGKDFKGFVRFGFCCETDVLEIGLEKMRLFMKKGYRTVPLADEESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPG9</accession>
    <name>A0A8H6CPG9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008674</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the clathrin heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155540.1">
        <property type="nucleotide sequence ID" value="XM_037299539.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337069"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071439">
        <property type="term" value="C:clathrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000002">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000005">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000082">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.730:FF:000003">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.110:FF:000003">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.110">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055358">
        <property type="entry name" value="CHCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022365">
        <property type="entry name" value="Clathrin_H-chain_propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016341">
        <property type="entry name" value="Clathrin_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF1">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00637">
        <property type="entry name" value="Clathrin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13838">
        <property type="entry name" value="Clathrin_H_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01394">
        <property type="entry name" value="Clathrin_propel"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002290">
        <property type="entry name" value="Clathrin_H_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="543"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="694"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="842"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="988"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1137"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1283"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1432"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1623"/>
            <end position="1680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1623"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1652"/>
            <end position="1674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6227232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1680" mass="190322" checksum="838CDE5D9D57D898" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ28</accession>
    <name>A0A8H6CQ28_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155717.1">
        <property type="nucleotide sequence ID" value="XM_037299716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ28"/>
    <dbReference type="GeneID" id="59337248"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF94">
        <property type="entry name" value="PHO85 CYCLIN-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="49280" checksum="5AA0A6010D871747" modified="2022-01-19" version="1">MASGDVHQMSSAISNPFRTPSQVSHLQNDPTATPSPTSNSRSSHTLQPTSPANVRSIPHIDGPHIHWRGAPPQEQQHINDGNHLMPQESQSEAIPPPSPRLEPTHRSTLSLNGSTVNSPSRIKVRDLGHIQSFASEEVLTRSRHPSRHSLRGRMDGGQQYEISGMPVTDVIEMVAGLLTKIITTNDRQHENLHRNIPPPEGTTALSPQATSVLAFHGKNVPSITIASYLSRIHKYCPTTYEVFLSLLIYFDRMTEMVNSGALPRLPGDGQHGRGHSRVPSGVSSNGSSETAVNSSPGGAGIDSSPRAPTIVSPPGSDSSDSQDSTMRPSPSMPSAIPDSDAYDLSHYFVVDSFNIHRLVIAGVTCASKFFSDVFYTNSRYAKVGGLPLNELNHLELQFLLLNDFRLSVPIVELEAYGTMLVEFYAREVISQQQQANHMEGLYMQTQTPTPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQE2</accession>
    <name>A0A8H6CQE2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008870</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155734.1">
        <property type="nucleotide sequence ID" value="XM_037299733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQE2"/>
    <dbReference type="GeneID" id="59337265"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000034">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000068">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF19">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01812)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="66"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6227426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="512" mass="56405" checksum="2CF876CF3CF42F78" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRI2</accession>
    <name>A0A8H6CRI2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Elongation factor 1 alpha-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Elongation factor 1-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_007957</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the Dom34-Hbs1 complex, also named Pelota-HBS1L complex, composed of dom34 and hbs1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156161.1">
        <property type="nucleotide sequence ID" value="XM_037298825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRI2"/>
    <dbReference type="GeneID" id="59336354"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990533">
        <property type="term" value="C:Dom34-Hbs1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002184">
        <property type="term" value="P:cytoplasmic translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16267">
        <property type="entry name" value="HBS1-like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000204">
        <property type="entry name" value="Translation elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015033">
        <property type="entry name" value="HBS1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08938">
        <property type="entry name" value="HBS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Tr-type G" evidence="14">
        <location>
            <begin position="356"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="124"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="286"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6228227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="767" mass="83363" checksum="D1A0495130BDE66C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRR3</accession>
    <name>A0A8H6CRR3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156413.1">
        <property type="nucleotide sequence ID" value="XM_037299077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRR3"/>
    <dbReference type="GeneID" id="59336606"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034808987" evidence="1">
        <location>
            <begin position="17"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="320" mass="33880" checksum="A223E35DB9E15316" modified="2022-01-19" version="1">MCLFASLLLVAGAAKALVPISPLDIQPSHPQPSGGNNSLNETSDTAASSAWLDEASSEMTASSLSMTNSTLVLNSVSAEPKFMCLDRFGSNLNPRSCQSAAFAIGDLSTSLFTWGPRGTAVTYDFPMPQRSLAEIHFAADGTCIIAALIGPRSTFALATLQDMAMGANTVIQQCVEQKSPNEGGIVKELGDGRLGVIVAKQTPNVRCYGSAAVMGIVNSCQSIMDKMDVSKEVRTFGTGGVGVNVKLPYTWYSVDNKCIMTLRTSGPSDSESWHRVWETATFVTAMCARQGKKGLHSQVGTNKNLFLEVAAEGMADVETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7Z4</accession>
    <name>A0A8H6F7Z4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006426</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147449.1">
        <property type="nucleotide sequence ID" value="XM_037297324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7Z4"/>
    <dbReference type="GeneID" id="59334827"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034139356" description="Carboxylesterase type B domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="2">
        <location>
            <begin position="52"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="609" mass="64241" checksum="60918AEA200823BD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8P2</accession>
    <name>A0A8H6F8P2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148531.1">
        <property type="nucleotide sequence ID" value="XM_037295833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8P2"/>
    <dbReference type="GeneID" id="59333327"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="89"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="240" mass="27794" checksum="2C7B092A19D11AA3" modified="2022-01-19" version="1">MHIRPATPSDFPSAASISVLCFWNDELYDYTNPWREQHPDHFRSLFLRRHKIRFVTPGYVFHVAVTDEGDQNHKGEGKVVAFAVWERTGTSKVAMVWRKDTFWKLLERTLLTAQEYYLSLLRADKSLHYARLAHFLAEAKSDFASIPEMWKMQNLAVHPNFQRRGIASMLLNWGKQQAGKEGVPIGLEASELARPLYLKNGFRKFGNMHIEDFPIEDVPIFLWEPRGLEGMWGMKGDVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F906</accession>
    <name>A0A8H6F906_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoethanolamine N-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.103</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003582</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine = phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25325"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25326"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-methylethanolamine phosphate + S-adenosyl-L-methionine = N,N-dimethylethanolamine phosphate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25321"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57781"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:25322"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.103" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149192.1">
        <property type="nucleotide sequence ID" value="XM_037294504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F906"/>
    <dbReference type="GeneID" id="59331993"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000234">
        <property type="term" value="F:phosphoethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307:SF2">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="304" mass="33485" checksum="248707B50CB31579" modified="2022-01-19" version="1">MPVGPSEEESPREIKDNETNPYISSIALYNLSRAHTLLASLATKPKWTNADTSSVDCMHYDGDTALEHCASQLDLQPNQHVLDIGSGFSATGRFLASKYGAHVTGIELQRENHELAQRITVRNVDPRVVERVRSVNADFLRLTPESLVVVGGDGKPSSARFDHVVSLMCIMHIPESARSAVFRQAARFLKAGGKMYIEDLYDKSCQRDAVSHLTERELHQLREIVACPYLPSASGYVADVAAAGFDGVAFEDVSEKWTGLVRARTENYRGSEKPNADLQKFYDTIAEVFEGGSVGGVRLTAVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F948</accession>
    <name>A0A8H6F948_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003702</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">RNA-binding nucleolar protein required for pre-rRNA processing. Involved in production of 18S rRNA and assembly of small ribosomal subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149312.1">
        <property type="nucleotide sequence ID" value="XM_037294624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F948"/>
    <dbReference type="GeneID" id="59332113"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF48">
        <property type="entry name" value="MEIOTIC COILED-COIL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="6">
        <location>
            <begin position="431"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="494"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="530"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="678"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="722"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="158"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="814" mass="89339" checksum="942A9A32BE573A59" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F990</accession>
    <name>A0A8H6F990_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycine zipper 2TM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149432.1">
        <property type="nucleotide sequence ID" value="XM_037294744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F990"/>
    <dbReference type="GeneID" id="59332233"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37014">
        <property type="entry name" value="EXPRESSION LETHALITY PROTEIN HEL10, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G06580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37014:SF10">
        <property type="entry name" value="RICH PROTEIN MS8, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05650)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="20433" checksum="E4525BE302AB55AD" modified="2022-01-19" version="1">MSNPYYGQGQPNGYNPAGNSGGRYEPDYTPANNNNSNNNNNYAPSPDMSNGRRYDLEPRTRRDHRKAPRYASSSSSGSSSEDERPAHRSAKKGAAHKDPSSSSSNRLDKAKEHFSTSDRGVGAGMIGAVAGAVIAQEAAQRNGKGSIGATIAGLVLGGLAGNALERGYDKKHEEKRAVTEKSSDRRRNHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9A2</accession>
    <name>A0A8H6F9A2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003566</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149176.1">
        <property type="nucleotide sequence ID" value="XM_037294488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9A2"/>
    <dbReference type="GeneID" id="59331977"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040153">
        <property type="entry name" value="Rcf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018:SF3">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="7">
        <location>
            <begin position="88"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="216"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="262" mass="29452" checksum="15E93CB88D0026C1" modified="2022-01-19" version="1">MKILTKEEEQAHYNETLKGGALGGTVGLALGVGGVFAAAARYPAFRQLTLPLRAFLCTSSATFGAIVTADRFSRSFEASRHPDEGYQDEAARMRAEQEAKKSSFERFMDLGKEYRYPIVGTSWVLGMGIALGVVGRSPYLSTQQKIVQARVYAQTITIAVLCATAVFEVGDRGKGEGRWETVKIVDPNDPTHKHLIDKKIHHERYEGEDQWRDMVEVEERKMKEREAAAHEQDKQHHKQHAKKKPEHEEEKNNEKKGGGDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA74</accession>
    <name>A0A8H6FA74_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150091.1">
        <property type="nucleotide sequence ID" value="XM_037294013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA74"/>
    <dbReference type="GeneID" id="59331501"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021822">
        <property type="entry name" value="DUF3405"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36205:SF4">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36205">
        <property type="entry name" value="CHROMOSOME 19, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11885">
        <property type="entry name" value="DUF3405"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="187" mass="21279" checksum="B02B57F90BE32966" modified="2022-01-19" version="1">MTILFNQDLLKVWYPGIDQGGGQSLHINQPLQFFSNMNPDFVFVWQFELDVRYTGNWYNILENAESREDPPRRSLMVTPGVRLSRQLLQIMHDAQSMNIRSQTFAHTTVLLHALKVAQFSVPQYLDHQEEVDEPEEDNKIFNTVGPNGTFTDFAAQDIDQNANTKYVSVASGVRAVSTDVVSAVVWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAH1</accession>
    <name>A0A8H6FAH1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150737.1">
        <property type="nucleotide sequence ID" value="XM_037293084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAH1"/>
    <dbReference type="GeneID" id="59330571"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23812">
        <property type="entry name" value="UBCc_ScPEX4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="10"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="163" mass="17887" checksum="97B387808F54706D" modified="2022-01-19" version="1">MATSRSPTPPSTKRLLQELKSIVEDPSPILQSLSPASEAEILHWEAVMKGFPGTAYEAGLWHLDILIPPSYPLAPPKITFTTPICHPNVDFKTGEICLDLLKTSWSPAYTLTSTLEAVHQLLAYPEVDSPFNVDIATLLKSGDKIGGESLVRFWCAERRWGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB69</accession>
    <name>A0A8H6FB69_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_002319</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150898.1">
        <property type="nucleotide sequence ID" value="XM_037293245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB69"/>
    <dbReference type="GeneID" id="59330732"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="9">
        <location>
            <begin position="144"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="9">
        <location>
            <begin position="224"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="450"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="559"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="454"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="646" mass="70523" checksum="27C1FCF0BD96C442" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB71</accession>
    <name>A0A8H6FB71_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF6536 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001123</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151472.1">
        <property type="nucleotide sequence ID" value="XM_037292053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB71"/>
    <dbReference type="GeneID" id="59329539"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046623">
        <property type="entry name" value="DUF6536"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395:SF1">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20163">
        <property type="entry name" value="DUF6536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="510"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="636"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="681"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6536" evidence="3">
        <location>
            <begin position="85"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="723" mass="79553" checksum="E20422E1CA416FF8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB74</accession>
    <name>A0A8H6FB74_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha,alpha-trehalase</fullName>
            <ecNumber evidence="3">3.2.1.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001659</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 65 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151126.1">
        <property type="nucleotide sequence ID" value="XM_037292587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB74"/>
    <dbReference type="GeneID" id="59330073"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000032">
        <property type="entry name" value="Vacuolar acid trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.40">
        <property type="entry name" value="Glycoside hydrolase, family 65, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.420.10">
        <property type="entry name" value="Maltose phosphorylase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037018">
        <property type="entry name" value="GH65_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005194">
        <property type="entry name" value="Glyco_hydro_65_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005195">
        <property type="entry name" value="Glyco_hydro_65_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005196">
        <property type="entry name" value="Glyco_hydro_65_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051:SF8">
        <property type="entry name" value="PROTEIN-GLUCOSYLGALACTOSYLHYDROXYLYSINE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03633">
        <property type="entry name" value="Glyco_hydro_65C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03632">
        <property type="entry name" value="Glyco_hydro_65m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03636">
        <property type="entry name" value="Glyco_hydro_65N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034882904" description="alpha,alpha-trehalase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 N-terminal" evidence="9">
        <location>
            <begin position="61"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 central catalytic" evidence="7">
        <location>
            <begin position="424"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 C-terminal" evidence="8">
        <location>
            <begin position="768"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1040" mass="112303" checksum="67663C1774FFD487" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBF4</accession>
    <name>A0A8H6FBF4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151635.1">
        <property type="nucleotide sequence ID" value="XM_037292216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBF4"/>
    <dbReference type="GeneID" id="59329702"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034678523" evidence="1">
        <location>
            <begin position="23"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="209" mass="22383" checksum="9E68FF6CAD44CA47" modified="2022-01-19" version="1">MYVWGKPHGFILSVILISIAVAAPSAIPVFKNDDVSANAPPQPAPSTEALCPDPLLPRIPVMRQLPAGVHVTVAHGWVITARAIASFIVPVQTMAAVLDSFYHDALVQLATSSFENKPHPGHAFGLSIGSVNLALRAVNSGDYLTWEACEVVVASLLHDAQMGFTGQFRSEWYHAESGVLLFGAKGAVRNDKEAAVEGDRDAIEDEEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC67</accession>
    <name>A0A8H6FC67_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000789</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152087.1">
        <property type="nucleotide sequence ID" value="XM_037291725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC67"/>
    <dbReference type="GeneID" id="59329207"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF10">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 29-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="13"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="89"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="16505" checksum="CC39D4B74A08BDB2" modified="2022-01-19" version="1">MDQGAVTEVRDRDGDTALLPAVRNGHRETARVLLEAKANAHTLNRDGSRPSAEVCARDACRHANLGTVELLLDSGADTERAGSGALGDSGRTAMMRAAIRGHYDVVGIMVEGRANVDVKGNDGCSVWGRAEKDGHFDLCEASATERRKLSPPPAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEA2</accession>
    <name>A0A8H6FEA2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBM21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010382</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154052.1">
        <property type="nucleotide sequence ID" value="XM_037301235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEA2"/>
    <dbReference type="GeneID" id="59338773"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF36">
        <property type="entry name" value="GLYCOGEN-BINDING SUBUNIT 76A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="349"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="669"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="718"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="769" mass="83772" checksum="1C64AD2743209657" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEN7</accession>
    <name>A0A8H6FEN7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_009728</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154436.1">
        <property type="nucleotide sequence ID" value="XM_037300588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEN7"/>
    <dbReference type="GeneID" id="59338123"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000095">
        <property type="entry name" value="Triglyceride lipase-cholesterol esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="10">
        <location>
            <begin position="134"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="465"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="545"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="565"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6225727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="599" mass="66587" checksum="2149AADC71A60876" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF19</accession>
    <name>A0A8H6FF19_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009727</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154435.1">
        <property type="nucleotide sequence ID" value="XM_037300587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF19"/>
    <dbReference type="GeneID" id="59338122"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="169" mass="19046" checksum="3768F95F8424E040" modified="2022-01-19" version="1">MKQCKVTQLVNTFKIELREAEASGIRALAKSIELQVVLIILANKLFSDLSSADIALGLTNRYSHLVRGYRYVWMFPFTEMHVELVWNRSQVAQTEPFKVLAEKNGQQMSVRRFLMAFMRDVVAGRADKTLVLDPRSCGNERSAANLVEDVIGLFMSCVDSKDILGALMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFC5</accession>
    <name>A0A8H6FFC5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154507.1">
        <property type="nucleotide sequence ID" value="XM_037300659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFC5"/>
    <dbReference type="GeneID" id="59338195"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="132" mass="14317" checksum="6153D07DE539046F" modified="2022-01-19" version="1">MDVLGDVAVLALPYPFSLDPSKGNTNHFATPPNFWTTAEVSIGLLAACMPPLNPLIRRAPSSLNIDDSINTSLALRRSDRSKTSERLSSVEITPKIHEFNEGKMVATKRADIEMSDYGSIEVDESASIGKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFQ1</accession>
    <name>A0A8H6FFQ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009039</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Dual chitinase/transglycosylase that plays a role in cell wall architecture. Chitinase and transglycosylase activities are coupled. Required for the polysaccharide cross-linking at the septa and the cell wall. More specifically, transfers chitin to 1,6-beta-glucan in the cell wall.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154726.1">
        <property type="nucleotide sequence ID" value="XM_037299900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFQ1"/>
    <dbReference type="GeneID" id="59337434"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000159">
        <property type="entry name" value="Glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF22">
        <property type="entry name" value="GLYCOSIDASE CRH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GH16" evidence="7">
        <location>
            <begin position="169"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="438"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="476"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="507"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="561" mass="59191" checksum="8E7A37D030F6EEAC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGJ1</accession>
    <name>A0A8H6FGJ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008894</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155758.1">
        <property type="nucleotide sequence ID" value="XM_037299757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGJ1"/>
    <dbReference type="GeneID" id="59337289"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019368">
        <property type="entry name" value="Ribosomal_mS29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10236">
        <property type="entry name" value="DAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="476" mass="52393" checksum="C25A1D45DD079893" modified="2022-01-19" version="1">MSLQTCWRCIARASCQPCPTRRPPQQLCTLYPASTAAFTTSPSLSALPPKKKAKSPMVEVNVKGAKKTFTKKKKKLQKVDRGKKPAVGERKALRKRVVLSNTNALEVEGLKDITAQSMIDDSLRGHVLAIPGPVVDQLRAVEAFKVTQGWPLFRRPSMLIRKDTLDMATEIENISLGIGDKTIRRVFVGERGSGKTTMLLQAMTMAFLKDWVVINIPQAQDLILAQTEYGPLPSTTPTLYTQRTYTASLLNAISRANPILQTLQLSQPPAENEIPIPVPPNISLSRLALLGAEDPEIAWPIFELLYRELTLPDRPPLLLCMDGLAHAMRNTHYISNTYQPIHAHQFTIIKWFLDHLSGTSPLPNAGLVLASTSESNSPVVPALKLALGQLEGNSAVRKDPFAKYDERVLGVFEGGGVEVKRMGGLSKEEARGLMEYWALSGVVRQRVDDGFVGEKWCLSGGGCVGELERAVVRMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGY9</accession>
    <name>A0A8H6FGY9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mannose-1-phosphate guanyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GDP-mannose pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">GTP-mannose-1-phosphate guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_007917</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell-size checkpoint.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156121.1">
        <property type="nucleotide sequence ID" value="XM_037298785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGY9"/>
    <dbReference type="GeneID" id="59336314"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06428">
        <property type="entry name" value="M1P_guanylylT_A_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="11">
        <location>
            <begin position="314"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6228187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="443" mass="48654" checksum="60F5AFB5554DA08E" modified="2022-01-19" version="1">MSLKVPTRQANGTSATTASTKAVILVGGPSRGTRFRPLSLDVPKPLFDVAGHPIIWHCLRAVAKVPGIREVILVGYYDESVFREFIKDSARELPQIRIQYLREYQALGTAGGLYHFRDAILKGGPDRFFVLNADVCCSFPLEQMLKLFEEKKAEAVILGTRVSNDAATNFGCIVSDAQTKQVLHYVEKPESHISNLINCGVYLFSTETIFPSIRSAIKRRADRPRLVSYPSSENLDSSFFAEKLEDGEKNEVLRLEQDILSDLADSNQFFVLETKDFWRQIKTAGSAVPANALYLQKAFQSQSEELAAPSANILPPVFVHPTADVDPTAKLGPNVSIGPRAVIGAGVRVKESIVLEDAEIKHDACVLYSIIGWNSRVGAWARVEGTPTPVGSHTTSIVKNGVKVQSITILGKDCGVGDEVRVQNCVCLPNKELKRDVTNEVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI62</accession>
    <name>A0A8H6FI62_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-splicing endonuclease subunit Sen54 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007075</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156605.1">
        <property type="nucleotide sequence ID" value="XM_037297970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI62"/>
    <dbReference type="GeneID" id="59335475"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024337">
        <property type="entry name" value="tRNA_splic_suSen54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024336">
        <property type="entry name" value="tRNA_splic_suSen54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21027">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21027:SF1">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12928">
        <property type="entry name" value="tRNA_int_end_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen54 N-terminal" evidence="4">
        <location>
            <begin position="71"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="383"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="410" mass="45656" checksum="A281E550B1CF7060" modified="2022-01-19" version="1">MADIDEDSTIRATPDPTDTDLSDETQDFRFLSTLSQPTDHPTLPRRGEKDFEHHGTLSQTDTLAASRNAMHDALSVSRTHNPKSLIVGLYDPSDDSTTIEQPKGPNLRTMGKADSRGRLSLLPEETLYLVERGSLDLRWRGEELEGLPLSLQAAYAYLLGKRGLTLERYTVYAGLKRSGYVVQRGPAWYPEDYDGDFIPPRELEKPKPLGLFAHFYKALFDKPLPDAAPSGPLVTPGFYRSYDSIYRLMTLIPAHDPAEPTDRESLRSSASMGHPTSPTHPRIRPAFYVWKPTSEFKKSAPPPPDFRIAVINAREESFPVLEQLDDLLQSVPYDPPPPGSEGQTYRRVKHGWRNVLLAVVDQGVVSYMRIADAGFGKEKVYETRGRGRGGKRGGFHGRGRGGGRGRGKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIE1</accession>
    <name>A0A8H6FIE1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_007219</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156747.1">
        <property type="nucleotide sequence ID" value="XM_037298112.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17959">
        <property type="entry name" value="DEADc_DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF2_C_DEAD"/>
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    </dbReference>
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        <property type="entry name" value="ATP-dependent RNA helicase DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR033517">
        <property type="entry name" value="DDX54/DBP10_DEAD-box_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
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        <property type="entry name" value="DEAD_box_RNA_helicase"/>
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    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF8">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08147">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01123">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="119"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="358"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="334"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="652"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="814"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="354"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="814"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="845"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6229105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="914" mass="100740" checksum="56A1F15612095431" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIH8</accession>
    <name>A0A8H6FIH8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156805.1">
        <property type="nucleotide sequence ID" value="XM_037298170.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59335678"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="199" mass="22924" checksum="919C4878EC9AA697" modified="2022-01-19" version="1">MPGDSGTIEKLPKELLLRILRHLYPPAAFQPFDMESNCQEDSAWHDRLLSAHFDIVNFSKVPRLFHALSVSLAQSHSRFITEEKKKGLILHAVNEFSLAYVEKTGMMRKLERVFQGGMRTITSDIVAKEAAEHFDPLSWLYVGYNHTRRAMRCRHIIRRPGKLVRDGHIGFGKDKWLVRWLVVLETPEQLPPQVLAVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIV3</accession>
    <name>A0A8H6FIV3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007489</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157015.1">
        <property type="nucleotide sequence ID" value="XM_037298380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIV3"/>
    <dbReference type="GeneID" id="59335888"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="173" mass="19635" checksum="02D2620209A37E5F" modified="2022-01-19" version="1">MRNRRMPGDEMPGELGGIKIHPEFFIATKKGGISPQHVDNLGKMTCVMMLEGVKFWYIPAGDRDAIRRDHRAGDTAPGSVQLKIKLDVGKVFNEDASSDDWTTLTTIITFLDDRECFTEKESYRAYESLKNLNESPQLLSHFKSLENGSKLQQALKNFQTTAKKVLKKWQDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIW5</accession>
    <name>A0A8H6FIW5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclopropane-fatty-acyl-phospholipid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007439</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFA/CMAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156965.1">
        <property type="nucleotide sequence ID" value="XM_037298330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIW5"/>
    <dbReference type="GeneID" id="59335838"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050723">
        <property type="entry name" value="CFA/CMAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003333">
        <property type="entry name" value="CMAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43667">
        <property type="entry name" value="CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43667:SF2">
        <property type="entry name" value="FATTY ACID C-METHYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003085">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6229323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="50134" checksum="C37C5EF2D873AD30" modified="2022-01-19" version="1">MAIVSRTFDSLRSYLGSFTWGPFVKLSRSTVLGLLSRIEVGQLVVRDSDGTVTICGQPCIKDGSPRTELRVSKEAFWVRVMLFADMGFAESFMLGEISCPDLVAFFRIFILNRAQLSNATTLTSSLASTVNSLARSTNTMANSQLNVSAHYDISNAMFAAFLSDDMTYSCPIWSPTFDAKAQCESLEDAQGTKLQYFIDAAKIKASDHVLEIGTGWGSFAIAAVKKTGCRITSLTLSREQKELAEERIAAAGYTDNIEVLLCDYRALPTPREGLYDKLVSIEMLEAVGREFLATYFDCVNQLLKPQGGIAVFQCITMPETRYEGYSKGEDFIRKYIFPGGHLPTVSQLVGSIEQGSHGALVVDSILNIGGHYAKTLRLWREKFERNFDSEIRPALLGEHEGMTDKDVELFRRKWQYYFTYCEAGFNTKTLGDVVLTVGREGATEMLENIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJZ1</accession>
    <name>A0A8H6FJZ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157202.1">
        <property type="nucleotide sequence ID" value="XM_037295201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJZ1"/>
    <dbReference type="GeneID" id="59332691"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35205">
        <property type="entry name" value="NB-ARC AND TPR DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35205:SF1">
        <property type="entry name" value="ZU5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="2">
        <location>
            <begin position="19"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="652"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="773" mass="86874" checksum="30F69A9238A7A65B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKB9</accession>
    <name>A0A8H6FKB9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">mRNA 3'-end-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_004458</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">Component of the cleavage factor I (CF I) involved in pre-mRNA 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CPSF4/YTH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157376.1">
        <property type="nucleotide sequence ID" value="XM_037295375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKB9"/>
    <dbReference type="GeneID" id="59332866"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045348">
        <property type="entry name" value="CPSF4/Yth1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102:SF24">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="180"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="180"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU369008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6230119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="28937" checksum="771D8157C6658756" modified="2022-01-19" version="1">MAATVDHRGVTPMNFDHESPNEYQKRPTPEFAFSDFLKREYRFGLDPNRPVCQADRQGLCPLGNLCPDKHGNHHNNNLVCKHWLKGLCKKGDQCDYLHEYNIRARAECTNYNRSGYCVSGEDCNYLHVDPATRLPPCPHYEKGFCPLGQRCSKKHVRKTLCRFYLAGFCPYGRQCKEGAHPRFPEDLPKPTLKVEKTAEEIEAEKERLREEADRQEQREYERNESNRGRGRGRGGGGRYNPRRRGGGHDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKF1</accession>
    <name>A0A8H6FKF1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_004491</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157409.1">
        <property type="nucleotide sequence ID" value="XM_037295408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKF1"/>
    <dbReference type="GeneID" id="59332899"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17964">
        <property type="entry name" value="DEADc_MSS116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="53"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="270"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="495"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="507"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="551"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="568"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="586"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="600"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6230152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="611" mass="66134" checksum="329C7425C91F881A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKF8</accession>
    <name>A0A8H6FKF8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1746 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157427.1">
        <property type="nucleotide sequence ID" value="XM_037295426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKF8"/>
    <dbReference type="GeneID" id="59332917"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044695">
        <property type="term" value="C:Dsc E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038967">
        <property type="entry name" value="Dsc4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013715">
        <property type="entry name" value="DUF1746"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39405">
        <property type="entry name" value="DSC E3 UBIQUITIN LIGASE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39405:SF1">
        <property type="entry name" value="DSC E3 UBIQUITIN LIGASE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08508">
        <property type="entry name" value="DUF1746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1746" evidence="2">
        <location>
            <begin position="63"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="324" mass="35996" checksum="D2172965242CD4EF" modified="2022-01-19" version="1">MNDASPSASDHAPHDLNDDASNAHGIASAAAAVAANNDDDSVQRKRRRQREQKAAFLDHLIRNIDIIFYCQLSILYYMDISLFKFLLRALPHWFYFTPKPSIFPPPSPTHRPYIGMIFGNNIVCIILHLLLRPPAAGEATRGYLLGGLLIDFVGQESPVSKWKLLVLDTLVLALQVLILAVTLERKKLAVENGDVLVEEVEEARQDHDSEERGMLRRDDTVQDEIELQDIRHTSSGRTGGDEDRERDELLESDESTEPRDQHPLDPFITGQAVIANVRVIDTIRSQWLTADTSGESTASGAAAVAAVAGRTLGYRLGERLQQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKR1</accession>
    <name>A0A8H6FKR1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="2 7">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004623</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Serine hydrolase involved in the detoxification of formaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-formylglutathione + H2O = formate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the esterase D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="2 7"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157540.1">
        <property type="nucleotide sequence ID" value="XM_037295539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKR1"/>
    <dbReference type="GeneID" id="59333030"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000002">
        <property type="entry name" value="S-formylglutathione hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02821">
        <property type="entry name" value="fghA_ester_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061:SF0">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0719">Serine esterase</keyword>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR614186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6230283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="286" mass="31422" checksum="FEAA697723EE9ED6" modified="2022-01-19" version="1">MSVQTRATIASFGGKLLKLSHNASSTNCEMAFNLYLPPQTTTNPLHKVPLLIYLAGLTCTGDNGAEKGFFQHAASEKGIAVLYPDTSPRGLKIQGEDDSFDFGSGAGFYVDATKSPWNKGYKMYSYITSELPKTVFDNFKQVDPERISIFGHSMGGHGALTLFLKNPGKYKSVSAFAPIANPSNCQWGRKAFGGYFGEDEKAKWAEHDATELVKKWSGEPLDILIDVGTSDNFYKQGQLLPENFVEAAEGKGKVNVRWQDGYDHSYFTMATFADDHVGHAARYLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKR4</accession>
    <name>A0A8H6FKR4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_004672</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKN1/KRE6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157589.1">
        <property type="nucleotide sequence ID" value="XM_037295588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKR4"/>
    <dbReference type="GeneID" id="59333079"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02180">
        <property type="entry name" value="GH16_fungal_KRE6_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005629">
        <property type="entry name" value="Skn1/Kre6/Sbg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361:SF1">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03935">
        <property type="entry name" value="SKN1_KRE6_Sbg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="11">
        <location>
            <begin position="176"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6230332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="668" mass="73319" checksum="F5DB6A9A70C4DFFA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKX1</accession>
    <name>A0A8H6FKX1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glucose-methanol-choline oxidoreductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157645.1">
        <property type="nucleotide sequence ID" value="XM_037295644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKX1"/>
    <dbReference type="GeneID" id="59333136"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="51"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="126" mass="13866" checksum="01FAB64474F5BE35" modified="2022-01-19" version="1">MKKFLDFIELIEPLIGWGKDRPEWESFVKREADTCKSVPSVQRVSALPSADDYISGYHPAGTCKMGNDDDQMAVVDAQLRVRGVDNLRVVDASIMPTLMGGHPQVAVYAIAEKAADMIKRFAQAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL17</accession>
    <name>A0A8H6FL17_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157735.1">
        <property type="nucleotide sequence ID" value="XM_037295734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL17"/>
    <dbReference type="GeneID" id="59333228"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="205" mass="22539" checksum="EEC2AFC5C23555C6" modified="2022-01-19" version="1">MPSFMGPPYLPPGSPAYNAYFANPANRHPPSAYPTFIPPAPQPQIYVGKSSTHPPSDPPPHTPPQPQIYTGKSAPTTPDIDPRPVHQGGMSTYDQPGLANWVAANSFPDHPGPTDRHGPTATTKPHHSTTTAMPKPRSCLKKGVQFTPSVQARDERKQEGRNRTKSNGTRVKRMACKYCHVKAVGWTVDDEGYCDGCYRDSRERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL42</accession>
    <name>A0A8H6FL42_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157765.1">
        <property type="nucleotide sequence ID" value="XM_037295764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL42"/>
    <dbReference type="GeneID" id="59333258"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="335" mass="38819" checksum="9CA1F8B1FF95ED5B" modified="2022-01-19" version="1">MPLSLTRMPESSSTSQSQPAPAASTDPSTQQWTEVLEKGKPQRERSRKLGKPLENMIAEAIATARELGLENSPSRWQSGHHGWFFGKKYIEGKSFQTQRMKMVEIRNWPVKISTLPSEGQIMAYLTLLKTARVDQVAAYYRLHCEQIDKVRCCIARELIEHRENDKQYRQIFPVLFARHGHIAKLSKLKKAAHAVQVANKQYRLRAGIRAYARGLEMLIEKCEVRLQRSENFRPADWRSYGKRPRWADGRRGWTGLRPEEVDVRTVDTTKDEEVCSRDMRTEKLQGALRARYARSFPQAADRKLATSAIEDRRDREIVRAERTEQAKAILRKAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY55</accession>
    <name>A0A8H6KY55_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007103</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156631.1">
        <property type="nucleotide sequence ID" value="XM_037297996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY55"/>
    <dbReference type="GeneID" id="59335503"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027974">
        <property type="entry name" value="DUF4470"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14737">
        <property type="entry name" value="DUF4470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="27"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="581" mass="65801" checksum="EADDB8B123429D95" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZF4</accession>
    <name>A0A8H6KZF4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidase FUB9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Fusaric acid biosynthesis protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004183</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157103.1">
        <property type="nucleotide sequence ID" value="XM_037295102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZF4"/>
    <dbReference type="GeneID" id="59332592"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="9">
        <location>
            <begin position="10"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="217"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="331"/>
            <end position="335"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="354"/>
            <end position="355"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00083297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6229846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="412" mass="44936" checksum="4F1F34AEC6AEA74D" modified="2022-01-19" version="1">MAAQGKGEKPHDLNCLTIAELEKHAELRMNKQTRDYYNEGADSGSTLLENTTAYGKYRIRPRVLRDISLIDSSVSIWGACNSVPIGLAPTAMQCLAHPDGEIATAKACKEAGIAMGLSSFATSTIEDVASNIDGHPLIFQLYLFEEREHSRKLIQRAKKAGYKAIFLTVDTPMLGRRNMEIRNQFKLPKPYKLANFAEDSGSSASETVMGGSTPKSEQGEEKKSTGTKGYHDGKRRQAPTGPVSFHTHAPNPTLSWERDIGWLKEQCSPEMEVWVKGIATAEDAHLAVHHGVDGIVVSNHGGRQLNGALATLDALPDVVEAARGKIPVHVDGGIRHGTDVFKALALGADFVWVGRPVLWGLAYKGQEGVELCLRLLSDEFRLCMGLAGTVKVEDITKEYLVKIDKSGFTSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZV4</accession>
    <name>A0A8H6KZV4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004592</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157509.1">
        <property type="nucleotide sequence ID" value="XM_037295508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZV4"/>
    <dbReference type="GeneID" id="59332999"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04938">
        <property type="entry name" value="TGS_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.470:FF:000001">
        <property type="entry name" value="GTP-binding protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="488" mass="53603" checksum="4023320CAAE35F09" modified="2022-01-19" version="1">MPRDPLIGLVGKPSSGKSTTLNRFTTIDPQRAIGYLQISCACARHGVSDRCKPNYGACVQGKRSVPIELLDVAGLVPGAHEGKGLGNKFLDDLRHADALVHVVDVSGTTDAEGKATRGYDPSQDIVWLRSEIVRWIEGNLMEKWGSIKRRHVAIKANPVDTLQNQFSGYGSTSQTVARTLDHLKLKEPLEHWSPETISSVVDAFTDEKFPTVFALNKIDHPDADKNISRIAKMQDPQSIVLCSAISEVFLRKLAKQGFVRYVEGSEFVDTREDLLEMGEADGGGLKELDEKLKTRIENLKDMVLYRFGSTGVVQVLSRAAELLGLVPVFPVRNIHGFGSGAGSNFVFRDCVLVKKGTTVRDCARKVMGDAPLAYVEGAEGVRVSEDEIVEVGKNDVSCRIGSTLSRANVRDTGLVLQGRSMTTEVAQFLTTSLRCGPAEDHETSWRHASHYIGSYVDHNSMVQNNVLAYFGGLIVLLYRIDNRLNVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZW6</accession>
    <name>A0A8H6KZW6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004727</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157642.1">
        <property type="nucleotide sequence ID" value="XM_037295641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZW6"/>
    <dbReference type="GeneID" id="59333133"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="482"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="638"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="651"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="676"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="714"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="766" mass="84696" checksum="5B0D4BA8AC7FCF72" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L0A4</accession>
    <name>A0A8H6L0A4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000017</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157831.1">
        <property type="nucleotide sequence ID" value="XM_037290957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L0A4"/>
    <dbReference type="GeneID" id="59328436"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017000">
        <property type="term" value="P:antibiotic biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014503">
        <property type="entry name" value="Clavaminate_syn-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF56">
        <property type="entry name" value="TAUD_TFDA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019543">
        <property type="entry name" value="Clavaminate_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0045">Antibiotic biosynthesis</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="5">
        <location>
            <begin position="108"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="304" mass="33326" checksum="676D4D6E0A073CA0" modified="2022-01-19" version="1">MAGLPPSDIISEDMRVIAVPSESKLRLLNAMEGLPSPYTQLGPHCIASHNAVLSQLPPSLIADIRDFHSNSLAPGALLITGLPLDPDLPLTPANGERSTEKSSLVSEGCLTGIAKLLGEPFSYASEKNGDPIHNVCPVRHRETVQSNESSKVTLGLHVENAYFDKRPDYLALFCLRQDHENNARTSFVDARSALSKMDSMDRAELQKPVFIVPSPQSHHEATGGEKWSTPRPLFENLDDPKLMCHFPGMKATTPKSQEALDKFEETAQDIATHITLQPGSILLLNNQKVAHGRSHFEPRYDGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5L9</accession>
    <name>A0A8H6C5L9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146975.1">
        <property type="nucleotide sequence ID" value="XM_037297774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5L9"/>
    <dbReference type="GeneID" id="59335278"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034096587" evidence="1">
        <location>
            <begin position="19"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="303" mass="32909" checksum="9714F78FD987E87A" modified="2022-01-19" version="1">MFSKSVLVFMFPLALTFASPLQDAGDQTIQLSSVDFRSPANLSDDETAIRLNVSNEIRIQCDGEKYGFNPDVVDCQNARSYYKRSSTPFTYGERHSGHGINVFPLPYRLMGDEALCYLEPVLIDSSLGTGVASINQLSNAAYELILQCAVRQSKGGIATGIGGQNMAVVLGTYHPDVQCRGTFGPWASCRSILGDMPTLAATEIFGPPEDLAVQVPLPQFLESSDGKCMMRIFGRGGRDITSWFKVWEAATAVYAVCVRHQKVGSFRGLGELGNIFFTMTAQTPKLGNLSTTPMSTDDALSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C697</accession>
    <name>A0A8H6C697_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenine deaminase</fullName>
            <shortName evidence="7">ADE</shortName>
            <ecNumber evidence="7">3.5.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Adenine aminohydrolase</fullName>
            <shortName evidence="7">AAH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AAH1</name>
        <name evidence="9" type="ORF">HO133_006670</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenine + H2O + H(+) = hypoxanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.2" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C697"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000034">
        <property type="term" value="F:adenine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006146">
        <property type="term" value="P:adenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009168">
        <property type="term" value="P:purine ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01320">
        <property type="entry name" value="ADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000039">
        <property type="entry name" value="Adenine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01962">
        <property type="entry name" value="Adenine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028892">
        <property type="entry name" value="ADE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01430">
        <property type="entry name" value="aden_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43114">
        <property type="entry name" value="ADENINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43114:SF6">
        <property type="entry name" value="ADENINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="8">
        <location>
            <begin position="14"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6217568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="350" mass="39354" checksum="F59798ADE66C7148" modified="2022-01-19" version="1">MCRTHLHPYLAALPKAEHHMHLEGALSPSLLFALAARNSITIPTSDAAFGSPAALLDRYTRFSSLDDFLHYYFIGMKCLVKEQDFEQLAWEYFLKAKSDGVVHAEVFFDPQAHTGRGITYETVVNGFTTACQRAEKELELSTKLILCLLRHLPVEDSTKTYAQARMDWETGRLAGLGLDSSEKGFPPGAWKEIYGTAKTNGIRRTAHAGEEGPVEYIREALSELDVERIDHGIRLAEDTELMREVAEKKILVTLCPLSNVRLQCVKDVKELPIRTFLDNGVMFSINSDDPAYFGGYILDNYCAVQEAFNLTAREWAAITKAAVEGSWCNYSRKQYLLEQLNETMVQFGHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6A2</accession>
    <name>A0A8H6C6A2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Carbamoyl phosphate synthase arginine-specific large chain, mitochondrial</fullName>
            <ecNumber evidence="14">6.3.4.16</ecNumber>
            <ecNumber evidence="5">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Ammonium-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Arginine-specific carbamoyl phosphate synthetase, ammonia chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Glutamine-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HO133_006906</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="14"/>
    <dbReference type="EC" id="6.3.5.5" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000028">
        <property type="protein sequence ID" value="KAF6217436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146871.1">
        <property type="nucleotide sequence ID" value="XM_037297802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6A2"/>
    <dbReference type="GeneID" id="59335306"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01423">
        <property type="entry name" value="MGS_CPS_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000015">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="22">
        <location>
            <begin position="237"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="22">
        <location>
            <begin position="774"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="23">
        <location>
            <begin position="1039"/>
            <end position="1191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00068891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAF6217436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1194" mass="131223" checksum="D1AE470933CC77AA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7G9</accession>
    <name>A0A8H6C7G9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147695.1">
        <property type="nucleotide sequence ID" value="XM_037297121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7G9"/>
    <dbReference type="GeneID" id="59334623"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034156796" evidence="1">
        <location>
            <begin position="19"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="180" mass="18609" checksum="2D401E0A30EAF979" modified="2022-01-19" version="1">MQLTSIALFLSAAVSVSALPATLQARQAAAGAHSTTGPKSAKVACDRSSKSISRQIAEMSKLMASNISVPPYLAGYYTAIDDERQEIGCPGAVSLGKREFTPNKEPCDVNNGQNERMTALVIRFEADQIAVAPFVAGFMSAVLDEHKGLGCPPFTGPGSEAAAARIAGASNSTISDSDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7M1</accession>
    <name>A0A8H6C7M1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147559.1">
        <property type="nucleotide sequence ID" value="XM_037296985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7M1"/>
    <dbReference type="GeneID" id="59334487"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="248" mass="28349" checksum="F4C8C01B988427D8" modified="2022-01-19" version="1">MNIAFDSNANTAFWEPCDANTTCALEETFRDIEASLQKVETDMCKKNLVECNCPSIEDAYKSSDHYRRILQRSISDGLLYNDALVDSSGDDLFQNLRIAFYRTHIDMQTHKGFSSPGRPHITILSIQPHRAQSQKDLLCTEFWMMYYWANKLARRALHTTDHCLPIRMVSLCGYEARVVSAVFSLGYPRSVVRGKGPDPYQSAKCQIHIAEYRDLSNQQHFQDLMEELAATPCGNAEVQQKSPRKKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7W8</accession>
    <name>A0A8H6C7W8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005948</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148032.1">
        <property type="nucleotide sequence ID" value="XM_037296851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7W8"/>
    <dbReference type="GeneID" id="59334353"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050562">
        <property type="entry name" value="FAD_mOase_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47356:SF2">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47356">
        <property type="entry name" value="FAD-DEPENDENT MONOOXYGENASE ASQG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="10"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="439" mass="48788" checksum="BF7E5B0FC740F2EB" modified="2022-01-19" version="1">MEETKLRPFKVVIVGAGLSGLLLAHLLTQASIDFVILEAHKNVVHPAGGSFGCWPNAARILDQAGIWESVEKSGAPMGLNYIRKPNGSAFITSSLSSKIASVYGYDFRILERYRYHQILYENLPDKSKLWTGKRVTDIIEDCNGVRVVFENGSCQGDIVAGCDGVHSVVRQIMWEKANKLSPGLISAREKRSLTSSYRCLVGAAPAIAGIGANESNMIQNQGVYMVIVSQPGQTLFFVCIKEANVSHWPQRSHFTKEDAEKEAAKIADRPVTAHVLFGEIWMKRTRGYLTSLEDGVFEHWFFNRIVLVGDSAHKMSPIGAHGGNCAIESVAALANSLDRHLRLCSDTSANSKTIQSIFETYQRTREARVRKFAGSVHLMTRLVTWDGYTKKFVARYVLPWLNEVTFVGKLIKDAVKVDFLPIPSRSKGFFDQTSDEGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8U0</accession>
    <name>A0A8H6C8U0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004943</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148553.1">
        <property type="nucleotide sequence ID" value="XM_037295855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8U0"/>
    <dbReference type="GeneID" id="59333349"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034954642" description="Rhodopsin domain-containing protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="9">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="159"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="243" mass="27320" checksum="6585E8C275EB42B9" modified="2022-01-19" version="1">MAVWSMSTVLLVCFSCKPVAASFNYKLRYARTTVRKPESYDVENIFGFYNIMLLLMPMPLIWTLHMARAKKIGVSIVFANGGVIAAIAIVRQYILYTSTDAAGPSWDIVQIKDWITIEVNLAIICGCLPGLQPLFRKNPLLIPFLPFHLRSKFSGGHSAMEHSSWPKRLSGPRHAGGQKLEKGQNRQQAPWRMPDERSESGSSVQSMYHQQQQQYPLLQSALEPVAIRKESPRYEGNLQGAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C930</accession>
    <name>A0A8H6C930_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148658.1">
        <property type="nucleotide sequence ID" value="XM_037295960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C930"/>
    <dbReference type="GeneID" id="59333454"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="301" mass="32147" checksum="C392DCE7C270B05A" modified="2022-01-19" version="1">MVGIAATAWYTGDFANSECSLEFIPTRSTVTANVTNSSITVVKSSGSAATDPEPTGLLVNNTVTSLHFLSKMGPSLYVSVMGDAFEYNVHTTYKSSAYGNLTRREVGLRATEASFDAILDDILGIYGSGQIAIANETTSTTIAGTFGSYLFGKPLVLGLILPVNLVILLVIVIEGIRARFWRHLPQFDILDFKSTVTAASLGGNNISKKVQRQRGEGDKWTADAADRMLGDIKMCLPQGDFAEPRIILDGGLLMQDEADGGGTEMDDGMAPLETEPYTGAPAAWSPIGQHNEHSSGQRKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9E4</accession>
    <name>A0A8H6C9E4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004924</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148534.1">
        <property type="nucleotide sequence ID" value="XM_037295836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9E4"/>
    <dbReference type="GeneID" id="59333330"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034537064" description="Peptidase A1 domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="52"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="383" mass="40807" checksum="17CAD0CA85CD8A20" modified="2022-01-19" version="1">MFRTCLILAVLVMAPLIEALDLCVDDGECLRSPGQSPNGNGPVDTLYNPAQPVGTVTVGTKYNQSFEAVFDTGSYQTWFTTTGITGYAETGTPLHQNGSIVYGGNFTVEGPLYLDDVAFDGFTAKNIQVMAAQSTAFNATLGMGYWTKSTIGVAGFFETLIEQKSVTKPEFGLHLGRTNTPEKSSLTLGSRDSTKHTGEFVSVPVIQQGGWSIQIDGYSVNGKMLNSTTPVGTIPNPPLVDTGTPYINALHNAAVAIHSAIPGAVPADDTGNVWKYPCNTSEIYMPTLILSGTSLSIAPEDFNMGSAAGDGMCESAIRSPLAYGWVLGSPWLMSFYTVFHWGLSAKEWEVGNGTSVNFAPAVHQPARSQHHRLGVENVSEASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9F5</accession>
    <name>A0A8H6C9F5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004946</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148556.1">
        <property type="nucleotide sequence ID" value="XM_037295858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9F5"/>
    <dbReference type="GeneID" id="59333352"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="77" mass="7959" checksum="63A54C69C4F4D515" modified="2022-01-19" version="1">MSSRPDQSFRSRRMSVIVGVSLVVGGQDGDWRSVGLDLAGEESGSAAETEGIVEKAFVDADTGGTVKGESGKAVELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9P1</accession>
    <name>A0A8H6C9P1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148932.1">
        <property type="nucleotide sequence ID" value="XM_037294885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9P1"/>
    <dbReference type="GeneID" id="59332375"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="60393" checksum="AAB9F5AF961DF0C3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9W1</accession>
    <name>A0A8H6C9W1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glutamate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005076</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148686.1">
        <property type="nucleotide sequence ID" value="XM_037295988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9W1"/>
    <dbReference type="GeneID" id="59333482"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677:SF8">
        <property type="entry name" value="CYSTEINE SULFINIC ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="49768" checksum="61BF308ED5720C34" modified="2022-01-19" version="1">MSHQDSNSTHRSAEKEPVPSRADEVSNLLNAVQDLLIPFIRSADEDASTKKTGHGLSVQGGGPRTALVEHHKPHKLSQLMDLNLPQTGLAQAGFLEQVEQILQYSVNTWDRGFMSKLYASTDAPGLAAELILATLNTNVHTYEVSPALTMIEKHTTRALANLFGLNGPHAGGVSVQGGSMSNATSIVIARNTLHPDTKTHGNTANDLKLVLFTSAHGHYSVEKAAQTLGLGASSVRAVPVDPHTGRMDPSALSALVAAAKEQGQTPFYLNATAGTTVLGSYDPIGPLAAVCREHGLWLHVDASWGGPAVLSPAHAPKLAGSHLADSIAVNPHKMMGVPLTCSFLLGADLRRFHASNTLPASYLFHHDDDDDHASEPSSSSSSSDLHYYDLADLTLQCGRRGDSLKLYLSWIYHGSDGYAQQIDAAFATAAHLARLVASHPDLVSVGENPPPCCQGHEGSGAGARSEGFHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Z2</accession>
    <name>A0A8H6C9Z2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004116</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149082.1">
        <property type="nucleotide sequence ID" value="XM_037295035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Z2"/>
    <dbReference type="GeneID" id="59332525"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF134">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="53"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="316" mass="34957" checksum="7A105439816D941C" modified="2022-01-19" version="1">MDESPGKMYALATVLTVLATVAVTLRFYARRITKLGRYRNFVDFRCAGAAIGSLGRHTQVMPDGMPIFDGRMEVFFKIIYISQLTQTVTFGLTKIAVLLFYRRIFVPRTFSIISLVMIGLNITWTVAFFAANLLQCWPISGNWTGLDAAPGTCIDTTMMYLAQAWSDVFTDIVILSMPIPWIWRLQMAPMRKFFVVLMLQLGALVVCAGVAKLVVFHRITYDTAVGDEDLTYLLTPTVYWPMVESSLGIVGACLPLLRPVFTDSPAKSIFSSLRAMISLSSLRSNDARKPSGDYDKLEAGNVGASQNSLVDGAQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA60</accession>
    <name>A0A8H6CA60_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003948</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148916.1">
        <property type="nucleotide sequence ID" value="XM_037294869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA60"/>
    <dbReference type="GeneID" id="59332358"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="117" mass="12749" checksum="69FD8625CD98844B" modified="2022-01-19" version="1">MYPDCAYSNGNTIVQGPPDHPPISFEGFGAGTPFTTIGVHNELNRNPFIEDLLHIKFIAHTIFVIFPTISSTVSTYGYAVFIVIFAVGVTIAASSYNGNLTTVATYTEFVCKHARDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBZ4</accession>
    <name>A0A8H6CBZ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002463</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150218.1">
        <property type="nucleotide sequence ID" value="XM_037293389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBZ4"/>
    <dbReference type="GeneID" id="59330876"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF80">
        <property type="entry name" value="IP06716P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="5">
        <location>
            <begin position="36"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="181"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="274" mass="30346" checksum="8A41E5C2B9055860" modified="2022-01-19" version="1">MSPKSQQKAPRSRSHTPSRSQRAHSSNRSSASIQKTEILINVYDLLPPGTIASALWFIGSSLLHTGVVIKCLDREYAFGGHDRPGMSGVYWTKPHTEPPGGTFRCELLQGFTFRSEHEIENLIKKVSQEFQGTSYNLLSNNCNHFTSHLCEKLINKPAPAWLNRAASIGVALPCVVPREWIAPPDHDTADGELLEEDDEDERTSMLRRQYHRGSSFNGSTAGSAIASTMGSEEEEWDSDRDRRKGGSGKGKSAVRDTDDRIMPASERAPPLKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC11</accession>
    <name>A0A8H6CC11_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150097.1">
        <property type="nucleotide sequence ID" value="XM_037294019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC11"/>
    <dbReference type="GeneID" id="59331507"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="306" mass="34947" checksum="F0423A1507BA8A1D" modified="2022-01-19" version="1">MSIKYGTRDISPENNGPMRASMPPPPVAMAASKLRIQTLQEQLKQKDRIIYALEAQKRSRLAFEAEKARQKELAKMNMGWRRHNANPHTPWIYRSGEIRGGTEGNVKTEKAIFVTEYRFAIESVKKINAEAELAEARVQTLRAEQRDAYRKAQELIKVAEELEVKVAREMRVRVAQSSPKEDQDATRSDGDTTDSDGDVNMGEFRTATAPKNGRLVLNPPKRERSLSTENIRPTRLRLSQPKRERSVSVEEIQPTRFRLSQPKRKVGDESSEPVEAFRCTRAEKVRDESHRVRDGRVLKASAKARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC69</accession>
    <name>A0A8H6CC69_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149946.1">
        <property type="nucleotide sequence ID" value="XM_037293868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC69"/>
    <dbReference type="GeneID" id="59331356"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="264" mass="29219" checksum="CE2569681AA5794F" modified="2022-01-19" version="1">MASETDEDGVTSPYKTEISDPLEGYRQEEASDLFRITCEQYVADREANTKGTANPSVMNKPFWMFQVGPGGFPAWKARTTFGNAEDPFADFEDPVWCFTRYGATRTKLPDGRVVCIAGEHEDGYDPDFCIYNDVVVFDALQKSDPPPLLTPECIKIYGYPVETFPPTDFHTSTYFVDPTTGKESIIIVGGLGYTGQASRSQTDVYQLDLTDFSIQRLNTSGVGPTGGTSKHKAELIGGEGQAAIKVMTAESKVFTLRIHDMRWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCH0</accession>
    <name>A0A8H6CCH0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002599</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150354.1">
        <property type="nucleotide sequence ID" value="XM_037293525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCH0"/>
    <dbReference type="GeneID" id="59331012"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="172"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="321" mass="36546" checksum="65202DC7B0779D88" modified="2022-01-19" version="1">MANPEAYIPTVDLREYFEPTTPTSKDELVAKVRTACLEHGFLQIVGHGVPVTSQRRVLAACRTFFELPNESKTELSLYKNSWRRGYEGPGSQRPREDVLPDQKEGFFVGKHLPMDQVGFLKGPNVWPKDLADSEFREPVLEYYDNILNLGRKVWEVLVVSLGHQASILNRFTNDVAMFLKLLRYPEHTFTDPRQFGSGQHTDYGGITVLLQDPGKDGLEVWHAATKQWLLIPAVEDKFVINLGDMVQKWTGGEYKSTVHRVINKAGGERFAVPAFWHGDLEATNPLDPHDRSGETVSQHIYKKFYRDYSLPADTERESQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCN5</accession>
    <name>A0A8H6CCN5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002611</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150365.1">
        <property type="nucleotide sequence ID" value="XM_037293536.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331023"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015969">
        <property type="term" value="P:guanosine tetraphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75011">
        <property type="entry name" value="3-carboxy-cis,cis-mucoante lactonizing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="5">
        <location>
            <begin position="285"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="809"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="942"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="984"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1026"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1068"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1109"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1163"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1204"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1253"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1300"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1354"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1510"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1626"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1519"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1626"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1652"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1685"/>
            <end position="1698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1698" mass="191811" checksum="FFE9A0A6F1A879C8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCU6</accession>
    <name>A0A8H6CCU6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="9">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_002632</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150386.1">
        <property type="nucleotide sequence ID" value="XM_037293557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCU6"/>
    <dbReference type="GeneID" id="59331044"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031405">
        <property type="term" value="F:lipoic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000013">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000007">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050743">
        <property type="entry name" value="2-oxoacid_DH_E2_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178:SF5">
        <property type="entry name" value="LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="11">
        <location>
            <begin position="261"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="12">
        <location>
            <begin position="391"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="357"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6220951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="690" mass="75016" checksum="029C6D103EA5F4C1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD54</accession>
    <name>A0A8H6CD54_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150521.1">
        <property type="nucleotide sequence ID" value="XM_037293692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD54"/>
    <dbReference type="GeneID" id="59331179"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="313" mass="36247" checksum="4EA91203139E2ACF" modified="2022-01-19" version="1">MEHGEWPGADGFPVVMNTRPGRNRGRLGQTVYYQDGNGLLVPAGAAIGGGLHRSHSAAARRPAQIVINNAPYEDRSVSRSPHGHSHHRRSSYGRDHHYDDSEDEYRERASSPHRRRRPSRSHGSRSPSPYYDYELEKKMKKLEELEEKEKEEIAREKYEEEMILKEAKKAKKKKEEDEFKVKAIEEYNKKQAEEEVKKEKAKKEADEEFKERVKKTFGQAGYDEESIEKILKKGEKGKGHGHGKEIKIKDLTRPTYIKVHRKHVSPETLDEYNLPWEWDERDSNYIVIKQAQEGKGQSATGKEEVARKVQVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD66</accession>
    <name>A0A8H6CD66_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7905 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150514.1">
        <property type="nucleotide sequence ID" value="XM_037293685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD66"/>
    <dbReference type="GeneID" id="59331172"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057227">
        <property type="entry name" value="DUF7905"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25482">
        <property type="entry name" value="DUF7905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7905" evidence="2">
        <location>
            <begin position="474"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="831" mass="94752" checksum="11BE4E122B0F3742" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDV3</accession>
    <name>A0A8H6CDV3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome alpha-type subunits domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002206</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150786.1">
        <property type="nucleotide sequence ID" value="XM_037293133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDV3"/>
    <dbReference type="GeneID" id="59330620"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03750">
        <property type="entry name" value="proteasome_alpha_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="3">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="317" mass="34422" checksum="DA4FA2B4E07268EC" modified="2022-01-19" version="1">MADRYSFSLTTFSPSGKLVQIEYALNAVNQGVTSLGIKATNGIVLATEKKSSSPLIDPPSLSKVSLITPNIGMVYSGMGPDYRVLVDKARKVSHTGYKRIYNEYPPTRILVQDVARVMQEATQSGGVRPYGVSLLIAGWDDGVEPETQELADEGLETDAEKKKASGKTGGILKGGPSLYQVDPSGSYFPWKATAIGKSATSAKTFLEKRYKEDLELEDAVHIALLTLKETIEGEMNGETVEIGIVGAPANELLGYEGVEGAVGPRFRKLSKEEIEDYLTNLKRLFLSQLQIVSRSKEITTMGQTLLAIFSLPEDQPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE62</accession>
    <name>A0A8H6CE62_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein YOP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151257.1">
        <property type="nucleotide sequence ID" value="XM_037292718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE62"/>
    <dbReference type="GeneID" id="59330204"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF177">
        <property type="entry name" value="PROTEIN YOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="265"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="324" mass="35414" checksum="1126ABE11F079746" modified="2022-01-19" version="1">MGLFDIVPNLLSSIITILFPIFASYKALRTSDPAQLTPWLMYWVVLSCFSLVESWTWFILAWLPFYSSLRLLLLCYLVLPQTQGARFIYQTHIHPFLARHEHDIDNLIISAHDRAKSAGLTYLKRAIEFVKENLLGIQPKATPQTPSSQGGNYAQSLLSRFNLPSAREGLAAPAGDFYGLLSAALGTISATGGTGSREVQAEDLSRSGTLIPQGMTSASEKMTFLSTQRDRLRVLLSALDKEADGLGTEAAIERDVEKRLTGEQLGEGLRKSRSEAEFEEIGKDDLKGEGAKQQASGWMPWAWGAKGESGNVTKGKTSGVDAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CED5</accession>
    <name>A0A8H6CED5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151308.1">
        <property type="nucleotide sequence ID" value="XM_037292769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CED5"/>
    <dbReference type="GeneID" id="59330255"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790:SF4">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="445" mass="50245" checksum="7EE169C1ED373440" modified="2022-01-19" version="1">MCSECGDVHEPLCSYCGESCYGECNDPDVCPSCWVPANDPDDFDEAEFIDEAFRGCEDERCICQCHETFFRHEGWWVEAGVLKMEDVDGLPYLPYVRTGVFPFLKLPGEIRDKIYGSAFLQDGNQRKCASHRGNIHTALLGTCRQVYNEARHLPLTTNKLCFNSPIYAHDFLGFLLAPTQHDLVTGIHIEFYIGEFSNSSWALLLRELTKMPITHIGLTVKGKFPKEVVSEHVCFTNRFRVLKGLKTFDLILTPSCISKGDKKDIQETMRENLIKDYVRPKELTKSKAKRMVSTESNEGSKKPAKKVKKGNSMTKIFASPRSSRLGLKSQKSKRDAAKAAKERAKQNLLDQYNQLRQYAKSLDTDATPVKIRLEQAREAAEAIDETKFEKLAHGIFLTLEERYNKIAAARTNVPVFRAPVQSSEPTSSQELCQSLDSQSISQSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEU8</accession>
    <name>A0A8H6CEU8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151584.1">
        <property type="nucleotide sequence ID" value="XM_037292165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEU8"/>
    <dbReference type="GeneID" id="59329651"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13179">
        <property type="entry name" value="RanBD_RanBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000254">
        <property type="entry name" value="Ran-specific GTPase-activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045256">
        <property type="entry name" value="RanBP1_RanBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="2">
        <location>
            <begin position="110"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="255" mass="27952" checksum="EF5C75A65169F2D4" modified="2022-01-19" version="1">MSEINPDPSSTEASTTSPAKTEETQKDSAPAATSYTEMASNAAGSATTAAAGVKDSVFSMFGGGAKKEKKEEPDGETEDRSGSSKAKKDAEAAEADEGGEDDAPDSPDVHFEPVVHLTEKVETKTNEEAEEQTFKMRAKLFKFDRESREWKERGTGDVRLLKHRENKKTRLVMRRDKTLKVCANHYVVPDMKLSPNVGSDRSWVWNAAADVSEGEPEPQTLAIRFANSENANLFKEAFIKAQQENEALFTKGAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEZ7</accession>
    <name>A0A8H6CEZ7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151732.1">
        <property type="nucleotide sequence ID" value="XM_037292313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEZ7"/>
    <dbReference type="GeneID" id="59329799"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="113" mass="12098" checksum="59E84D7CB635191F" modified="2022-01-19" version="1">MSQTVSAVIVMTPTGGSSYVATVTTRTTEAAAAEPAGTSTAQMARNPVMWKARDIQRLGQLPLDSDDPAPARVEACITHLNNRVRDLPGDEDFPVTEEEDAVLKDIQRCEGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFN9</accession>
    <name>A0A8H6CFN9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152064.1">
        <property type="nucleotide sequence ID" value="XM_037291702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFN9"/>
    <dbReference type="GeneID" id="59329183"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053203">
        <property type="entry name" value="Cisplatin_resist-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022024">
        <property type="entry name" value="DUF3602"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693">
        <property type="entry name" value="PROTEIN PAR32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693:SF1">
        <property type="entry name" value="PROTEIN PAR32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12223">
        <property type="entry name" value="DUF3602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="148" mass="16018" checksum="542A0EAFD036A693" modified="2022-01-19" version="1">MMEYQKVGRGGAGNFYTPQDVEKASKETSKDVEAQRNAAGGGIEDLESQGRRFSTSGRGGAGNFTTRDMLAAGTTANIDVTPSLQENKPPELGHYGRGGAGNYRTPEPEKRRGEKDTLEAREKDVEIGLTEPEKAHLGKEGFDYDAHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG53</accession>
    <name>A0A8H6CG53_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin 3 binding protein But2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000609</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151910.1">
        <property type="nucleotide sequence ID" value="XM_037291548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG53"/>
    <dbReference type="GeneID" id="59329028"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034558459" description="Ubiquitin 3 binding protein But2 C-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="24654" checksum="BA330DAD8DDDE09C" modified="2022-01-19" version="1">MHFSLTTTLPLLSTLLSLTTAQQSTDANGCSTFEPTSAFQIYSDLPDLSVDLTIPNYSNLTNGTTNSTRRLSSRSKARSRPKSRRSAHIPFTFYVSQDAGNTNQQDLVVAFAGLPCGGPGPFSFEFNFQPQSAYFAQGQGQINMFRVDTLALGDAPTWNDIEPLTGSLVGTFELPAVGSEEPALLFLNQLVCQDQLVLRFGISRYSDEGGSVAYINAGGMGLRERNGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG65</accession>
    <name>A0A8H6CG65_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deoxyribose-phosphate aldolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152319.1">
        <property type="nucleotide sequence ID" value="XM_037291957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG65"/>
    <dbReference type="GeneID" id="59329442"/>
    <dbReference type="UniPathway" id="UPA00002">
        <property type="reaction ID" value="UER00468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046386">
        <property type="term" value="P:deoxyribose phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00126">
        <property type="entry name" value="deoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889:SF1">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="16871" checksum="FF8BEDF1EF6496CD" modified="2022-01-19" version="1">MVMNHVLLRQKHYSKVYTDIAAVRNVAPHPIILKVILETSQLSPREIIAGCKIAEAAGADYVKTSTGFNGQGATQENVRLMKSVVGDTIKVKASGGVKTVNECVVMMEAGAERIGTSNGVWIMDEAKGLLEQVTHAAATGELKGQRPASSLTRMFSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGS7</accession>
    <name>A0A8H6CGS7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000132</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152507.1">
        <property type="nucleotide sequence ID" value="XM_037291072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGS7"/>
    <dbReference type="GeneID" id="59328551"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF243">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA-2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="26"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="193" mass="21517" checksum="AF2CF80317829EA5" modified="2022-01-19" version="1">MYRQEFLSQLLSTENTVQADGAQEECMICSEVYGTMSTETGKVERQIRLPCDTKHTVGSDCIAKWLEEHNTCPVCRHEFFPARGPYYDDEDGSRRWVGNLEPDSDWDSEEYDSEDAQEEEYDADPTSIYGAEPLINLCHLLCNAIGFERDNHPVKRAAALVVSGILHLSILQDASPFSNFSIAAACVPGVADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGW2</accession>
    <name>A0A8H6CGW2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152506.1">
        <property type="nucleotide sequence ID" value="XM_037291071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGW2"/>
    <dbReference type="GeneID" id="59328550"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="64"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="156" mass="17108" checksum="38ED22CBDC0D91A5" modified="2022-01-19" version="1">MSESNEDAVKKPDYKDVEYNLPRNEEALPSIAAEHARLNDQAASYTDLMKDKIVHAPLKSPTEILDVGWGTGIVTRHLGSIYPSASVYGIDISPVPPTTASDAISSTPSNVESIIGDIRKVAGEDERLRAGNFDCIFQRLQRLCSDLYQRCLSLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHA3</accession>
    <name>A0A8H6CHA3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000371</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152745.1">
        <property type="nucleotide sequence ID" value="XM_037291310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHA3"/>
    <dbReference type="GeneID" id="59328790"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF3">
        <property type="entry name" value="COENZYME Q8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="6">
        <location>
            <begin position="515"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="138"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="398"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="208"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="270"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="351"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="863" mass="94568" checksum="34151B86FA3D51D3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIE2</accession>
    <name>A0A8H6CIE2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152990.1">
        <property type="nucleotide sequence ID" value="XM_037301356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIE2"/>
    <dbReference type="GeneID" id="59338894"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="534" mass="60415" checksum="8A68D201E76733E8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIR2</accession>
    <name>A0A8H6CIR2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010762</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153247.1">
        <property type="nucleotide sequence ID" value="XM_037301613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIR2"/>
    <dbReference type="GeneID" id="59339152"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="414" mass="47351" checksum="BB0BAE9A8ABCDB76" modified="2022-01-19" version="1">MSRPEKLHLALSSQSDVVLDLREGFVGKPQLTLRNEGADEITHCFPFHAELERNNDRDGKEDESLDLGRGLRCGALTITDESGSKIPLNYFGESGKGSYYELMSIMPGFAESTYGFRRISPELLELLGRHLIPERTYRVGFSGQPMGIMCDIGDVDPETHERQETEIEPSWDPREIPFTVVAGARKPRFTMSVTAPSSICHTSGIPGFHVVMLVTSLERRAVTVNVQLDAFADMWPQDPAHRWEKWGSYDMFRITRESDGWECLLNAKHPPEQTLRPAEDFRPANGLLEFNHGTTYTYHFHLGTEDLNHGIWPSYIHNEPYILRTNPQGFAAWDYGTEAELSRTHRTPSDWCRHGPILFEPVCDVALTIEAIMEERENPMPFFRLPLELREVVYGYVRFARYANRIRFTADLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIX6</accession>
    <name>A0A8H6CIX6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_011022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153505.1">
        <property type="nucleotide sequence ID" value="XM_037301871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIX6"/>
    <dbReference type="GeneID" id="59339412"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002838">
        <property type="entry name" value="Uncharacterized ATPase YjoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF132">
        <property type="entry name" value="ATP-DEPENDENT ZN PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="2">
        <location>
            <begin position="237"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="500" mass="56946" checksum="B660BA57CF8E3B76" modified="2022-01-19" version="1">MHTRPSTVPLRPSTSTSAVTSNSSRDGVYKEFYQNSTAPRIKSDVVLVEALRKQYPSLHVTVVPSYQCNFLVYAATGHASATPIDAENASTENLKHRQYMAPAKRMDGGSGFLYDSVQFGKWMYKWESKEYILYYIAGSDGPYNSPMNYLLGSTPMVNDELMLAACRWQNSIRKSVLVFDGGYWQYSSELWQSIQKANWEDVILDAEMKKNIIGEVTKFFNSKDRYKKLRVPWKRGVIFHGPPGNGKTISIKAMMHSLYDRDDPIPTLYVRSLASYGGPEHAISLIFSKARQMASCLLVFEDLDSIVSDAVRSYFLNEVDGLQNNDGIMMVGSTNHLERLDPGISKRPSRFDRKYLFPDPNMEERVKYCEFWRKKLDSNEDVEFPKALCEAIAGITKGFSFAYIQEAFVAALLVIANGDAGEEIKGEGPFGKEHEYKRCVCKKSDFEMMVRSKVEDEKHLEKYLLWREIKKVIKTLREELEMEKGVEGFACGFDCEKIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJN8</accession>
    <name>A0A8H6CJN8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009956</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153629.1">
        <property type="nucleotide sequence ID" value="XM_037300812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJN8"/>
    <dbReference type="GeneID" id="59338348"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF163">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="64"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="500" mass="54430" checksum="A83B1FFC9C4D9B99" modified="2022-01-19" version="1">MESYATEAPEIASDPAKFEKDSGFRSLEAESATQDRVDRHVVDFDGPDDPENPLNWSNTRKWCLVGLISAMTLVTNLATLMCAPAAPLILREFSSNNQLYQTLLVSIWELGEAFGPLFIAPLSEIYGRAPVYNAVNILFCIFSIAGAVSTNMHMFIAFRFLNGITVGAIVLNPCIVGDLFVTERRGNAMAIEGIAPLIGPVLGPVIGGYVSQAIGWRWLFWLAAIFAGVTEIGFLAFFRETYKVRILERKAARLRKVTGDDSYRSVHHVTHTIMGFLTVSVMRPARMLFLSPVIFLLGLYVALVYAYTYVLLTTMTEVFEQRYGFSTGSASLTFLGLGIGLTAGLLLCQATLDRYVSRAQTRGAMTPEQRLPPMIAGGLVIPAALLWYGWTVQARAPWTAPVVATATLGFGVAATVLPAFSYLVDAFGLHSASAVAATICLRCVAGAAFPLVAPSLYDRLGVGWGTSVLAVVALVFLPVPLLLMRFGERVRLRSRLLVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKI7</accession>
    <name>A0A8H6CKI7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein AF-9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010435</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154105.1">
        <property type="nucleotide sequence ID" value="XM_037301288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKI7"/>
    <dbReference type="GeneID" id="59338826"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16908">
        <property type="entry name" value="YEATS_Yaf9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573:SF1">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YEATS" evidence="7">
        <location>
            <begin position="11"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="162"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="28399" checksum="31A0373985317BF2" modified="2022-01-19" version="1">MPVPRYTKKDRVKGVQIHRPFVYGSIAKPLDPSNRPPGISPEHTHQWSVFVRGVNDEDISYWLKKVQFKLHETYTNASRMVESAPFEVTETGWGEFEVQMKLYFVPEANEKAQTLWHALKLHPYVGDVEGQKERRDPIVSQNFEEVIFSEPVEPFYDVLTSGPPLPARGKGGKGSKQATLRKQGERSAEIPYANSEGNPFSQKEEAMELDRLREAMKTLDGMVKEERAKLAGKEKVMEGLRREGAGQQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL52</accession>
    <name>A0A8H6CL52_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154131.1">
        <property type="nucleotide sequence ID" value="XM_037301314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL52"/>
    <dbReference type="GeneID" id="59338852"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="218" mass="24237" checksum="21EFDBBF9328E71A" modified="2022-01-19" version="1">MAYYNSPIVPTPFDLNLMTYLLNRIAHSHEVREQAEKQAENSCQAPPIKPSTHQIHCYSSNRPQTLSKQQQATTEKTAEKDKSTSSSSPVQVGTLSVISHQSTQPVQEKSLQQAKAENTAEKSSDNKATISKNTAQLSTIPRQIATQQAENDTTIWPAISACLGNITLRRIYCDDHSQITMINWTSASKGHTYLADVTPDGYAVYEEDEEVNQEHHGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLJ6</accession>
    <name>A0A8H6CLJ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF300-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009765</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154472.1">
        <property type="nucleotide sequence ID" value="XM_037300624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLJ6"/>
    <dbReference type="GeneID" id="59338160"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="256"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="329"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="38268" checksum="0450E0AA02C3877B" modified="2022-01-19" version="1">MVIEGGCVTVAMYCLIQFYIQLRRDLAAHSPFLKVVAIKLVIFLSFWQTFVISSITSTGAIKASSRIQTPDIKIGIPSMLLCIEMAIFSVFHLWAFPWKVYDVRRSQIVASESAAGFFPDPKTAYHGGPFGSKALMDAFNPWDIIKAVGRGFKWFFIGRKTRTEDISYKNSAQGTGLEPTRNQITAFETGNHSFDGSHPFVQVGKPARYQPLSEEEDSDRLLAHAQSNPEVPYPRPMERLPHLNAGGDIGTMGVYDPPTPHAPPYPTTRHQDPGQEYGVADERSLGEEDTGYHGARGRVTPVPPPDSHPLGPPGGRGNENEWNMWGGARENERDLGGDHGIGDNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM30</accession>
    <name>A0A8H6CM30_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009898</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154604.1">
        <property type="nucleotide sequence ID" value="XM_037300756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM30"/>
    <dbReference type="GeneID" id="59338292"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="16764" checksum="87DDA080C6B56059" modified="2022-01-19" version="1">MDSLEPPAGGDQNRGPELVAVIWVFTALASFVVSLRLFTRVKILKETALDDVFTILSLASPTLIPVYLRSVEADVIIIAACIPSLRPFALSVGQSLRDSNGKSRPKRAWYQQHSSDVPLALKPMVPGENGNGSNNSTEGGLSRASSTPPKVVQENH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CND1</accession>
    <name>A0A8H6CND1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154829.1">
        <property type="nucleotide sequence ID" value="XM_037300003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CND1"/>
    <dbReference type="GeneID" id="59337537"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031355">
        <property type="entry name" value="YBL010C/LAA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38698">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38698:SF1">
        <property type="entry name" value="FUNGAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17104">
        <property type="entry name" value="YBL010C_LAA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="569" mass="62023" checksum="AEF90DED392D6553" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNM9</accession>
    <name>A0A8H6CNM9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">L-arabinitol 4-dehydrogenase</fullName>
            <ecNumber evidence="11">1.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">GID complex catalytic subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glucose-induced degradation protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_009407</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-arabinitol + NAD(+) = L-xylulose + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16381"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17399"/>
            <dbReference type="ChEBI" id="CHEBI:18403"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RMD5/GID2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.12" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155094.1">
        <property type="nucleotide sequence ID" value="XM_037300268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNM9"/>
    <dbReference type="GeneID" id="59337802"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050019">
        <property type="term" value="F:L-arabinitol 4-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16652">
        <property type="entry name" value="dRING_Rmd5p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000143">
        <property type="entry name" value="Regulator of gluconeogenesis Rmd5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037683">
        <property type="entry name" value="Rmd5_dRing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044063">
        <property type="entry name" value="ZF_RING_GID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF12">
        <property type="entry name" value="L-ARABINITOL 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51867">
        <property type="entry name" value="ZF_RING_GID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CTLH" evidence="19">
        <location>
            <begin position="166"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="RING-Gid-type" evidence="20">
        <location>
            <begin position="358"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RING-Gid-type" evidence="17">
        <location>
            <begin position="358"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="502"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6226541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="894" mass="98285" checksum="3281CCD9B4C2BF59" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNR5</accession>
    <name>A0A8H6CNR5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008284</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155152.1">
        <property type="nucleotide sequence ID" value="XM_037299151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNR5"/>
    <dbReference type="GeneID" id="59336681"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15849">
        <property type="entry name" value="SNARE_Sso1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000008">
        <property type="entry name" value="Syntaxin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF380">
        <property type="entry name" value="SYNTAXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="295"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="55"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6226843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="401" mass="44084" checksum="B673DB2F05E1352C" modified="2022-01-19" version="1">MTFNLQVLTGDPQQQYGGYSNGGGGGYGQANPYEQTGNRYEQQEGRYDQESSNPYAQQASNPYASQPAAPQQAYGGRQQQGGYDTRYGQAQTTNGGSSTYPAGAGATGGGYVGGNDVEMTPMNGQAGAYGAQPSRDPNAILNECRDIDRGIDSIQRNLERLRFLQQRAIDDPDASQGTQTNRELDSLSSDTMTLYRSFAARIKTIKSQKESGDPRNKPQVGLVDRKLKSAINEYQMVEKDFRHKLSAQMERQYRIVRPDASDQEVQEAIQDTSNNQVFSQALLSQNRRGQSQSALRAVQGRHEAIQKIERQMMELAQLFQDMEAAVITQEPAIQNTEEKTENTVVNLDQGNKHLDGAVVKARSARRKKWICFWIVVLIVIIIGVAVGVGVGVTVGGTKKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP41</accession>
    <name>A0A8H6CP41_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">P-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008448</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155315.1">
        <property type="nucleotide sequence ID" value="XM_037299314.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336844"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000604">
        <property type="entry name" value="ABC transporter B family member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001471">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF15">
        <property type="entry name" value="ALPHA-FACTOR-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="806"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="849"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="923"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1034"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1069"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="53"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="382"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="808"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="1129"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="760"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6227007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1374" mass="150607" checksum="6F0CC77F11B99F8D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPI5</accession>
    <name>A0A8H6CPI5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155387.1">
        <property type="nucleotide sequence ID" value="XM_037299386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPI5"/>
    <dbReference type="GeneID" id="59336916"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="112" mass="12132" checksum="F7581E33B6122CAB" modified="2022-01-19" version="1">MPTPCAITLKCPKVPNPPGSSPPIRIKAVFQLQDSREEIYTGIMFEYLPAKIATKTAVIATTISDASPVIVSNYNGVSVRQADCGYRYLLPVKVADEPYVWEAGRATSAALM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPT1</accession>
    <name>A0A8H6CPT1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HlyIII-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008552</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155419.1">
        <property type="nucleotide sequence ID" value="XM_037299418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPT1"/>
    <dbReference type="GeneID" id="59336948"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF97">
        <property type="entry name" value="ADIPOR-LIKE RECEPTOR IZH3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="460"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="56310" checksum="0B9F014E3CA87D9F" modified="2022-01-19" version="1">MPCSTPSLTLSSHDGASDAHTTATPPPASPLSQSRRHSSHQPWRRHSHDLDSDVIFMKVDLFISELGRRLDWIENYGNLKLDATVSGAYFTLDAIRESCSHVSGELIGAGRRRARILVETVESRYNDALPTRETLEAKAQAGMRLTDKFLSDLEARALAVKDTGFSEIIDGGWRMAEEGFEKAREVVDEGLDKARRARETLKENVEHAVKRAKEHRLITYDDLPYPWQVNPHITRGYRFSENKIDCIKSAFNFSNETVNIWSHAIGLIIVLAIAFYFYPSSVNFSHSSKTDVFFAAAFFFAACKCLVCSCMWHTMSSISEQTLMERFACVDYTGISLLIAASIMTTEYTAFYCEPVSRWIYITTTATLGVGGVILPWHPTFNRADMNWFRVLFYVSLAATGFAPIFQLSWTRSPGWAWAFYAPIGKSISVYFVGALIYAGQVPERWLHGWFDYVGGSHNIWHLAVLGGILFHYMAMQSFFSVAFERGREECGYEILTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQL0</accession>
    <name>A0A8H6CQL0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phytochrome</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_007773</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155979.1">
        <property type="nucleotide sequence ID" value="XM_037298643.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336170"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009584">
        <property type="term" value="P:detection of visible light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000048">
        <property type="entry name" value="Sensor histidine kinase/response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.270:FF:000002">
        <property type="entry name" value="Sensor histidine kinase/response regulator, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013654">
        <property type="entry name" value="PAS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016132">
        <property type="entry name" value="Phyto_chromo_attachment"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013515">
        <property type="entry name" value="Phytochrome_cen-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043150">
        <property type="entry name" value="Phytochrome_PHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08446">
        <property type="entry name" value="PAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00360">
        <property type="entry name" value="PHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50046">
        <property type="entry name" value="PHYTOCHROME_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0157">Chromophore</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="1" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Phytochrome chromophore attachment site" evidence="13">
        <location>
            <begin position="447"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="14">
        <location>
            <begin position="823"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="15">
        <location>
            <begin position="1281"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="94"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="375"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1152"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1443"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1190"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1443"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1466"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="11">
        <location>
            <position position="1332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6228045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1513" mass="166912" checksum="62103FA0DDFD7C85" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQV6</accession>
    <name>A0A8H6CQV6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155857.1">
        <property type="nucleotide sequence ID" value="XM_037298521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQV6"/>
    <dbReference type="GeneID" id="59336048"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="209" mass="22813" checksum="00C1569736AFD2AB" modified="2022-01-19" version="1">MEDGVPTSGVWQWTISIITGVVGEAFQRYLLILNIITVASAIVFSIPVIALGLIVFLFIDSIAFFQSICAHPPVPTPPIATSSTNNSVEEVPQTYTDPPFADESLKIYTLPNTLRFIASPLDIDDVSTPLAYEVTIGQSVKQDDATSRVEVAPTGRVLGEVIGTYVDNVTVLLKMEDWFTESGTFEMGRWRVEQVQAGDELGMEVAVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQX3</accession>
    <name>A0A8H6CQX3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial</fullName>
            <ecNumber evidence="4">2.1.1.201</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ubiquinone biosynthesis methyltransferase COQ5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">COQ5</name>
        <name evidence="6" type="ORF">HO133_007695</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Methyltransferase required for the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-methionine = a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="EC" id="2.1.1.201"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.201" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQX3"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008425">
        <property type="term" value="F:2-methoxy-6-polyprenyl-1,4-benzoquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="179"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6227967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="316" mass="34805" checksum="4F88EAD22514147D" modified="2022-01-19" version="1">MSAPTLSRTSTRLTHRLLNPQPPYRCLSCCRYLAQSQEPANHSGPTPTTHFGFQTVPSSSKQSRVANVFTSVASSYDTMNDLMSLGTHRLWKDYFVRSINPGTRDCEEGKGKGQTILDIAGGTGDIAFRMLEHCTVVNKDHASKIIVSDINPDMLAEGKRRAREGGHDHSGRLSWLEANAENLHTISDNSIDLYTVAFGIRNFTSKALALREAFRVLKPGGVFACLEFSKVNNRLVEALYKRWSFGAIPLIGQLVAGDRESYQYLVESIERFPGQEELKGMIEEAGFLLPGGEEGKGWEDLMGGIACVHKGVKPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQX8</accession>
    <name>A0A8H6CQX8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007666</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155872.1">
        <property type="nucleotide sequence ID" value="XM_037298536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQX8"/>
    <dbReference type="GeneID" id="59336063"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11062">
        <property type="entry name" value="CYP58-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="52189" checksum="7DC17161B27D7C3E" modified="2022-01-19" version="1">MPITGSLGPIIRVAPDLLHVDDPEFFREVHGPQGKFLKSEYFYGTVGAPNALLSIRDPHEHKIRRAIVNPLFSQKSVNALASMVQDKLEQAMHIVRRRHLEGKPVDIQRLYRCISADIITTSMFGYSQDLIHFGDGTHPPLLTCLDGFLCHVHLMTHFPFLVNLAQKLPAIISKRILPGYVEFRKDCGHWIEKIMERREKGRYTDDNDRTVMFDLLLESNSEKDFQRLSLEQLIDEALIFITAGTDTTAYALSCATFRILHTPGVLAKLQEELGRVPLGKEGRFEWKYVQNLPYMTAVVKETLRISTPAIGPLPRVVPPEGARVQGNLIPGGTIILSTLITIHEDPHLFASPQQFLPERWLGDKGMELERWFVPFSKGSRQCVGKKYVHSKTDHNNARPTDVSCVFISLAYMELYLTLANFFGRFEMELFETDEKSMEWLDHGAALNASSVKVRAKPLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR59</accession>
    <name>A0A8H6CR59_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoglycerate mutase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156079.1">
        <property type="nucleotide sequence ID" value="XM_037298743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR59"/>
    <dbReference type="GeneID" id="59336272"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07040">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF54">
        <property type="entry name" value="PHOSPHATASE SPAC5H10.03-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18135" checksum="56BA77BD71EDF340" modified="2022-01-19" version="1">MAPVIHCVRHAQGYHNLSVANHSLPDPSLTPFGEEQCHKFAQDFPYYQSIGAIVASPLRRAIYTALYGFKPLIEKGMSVIALPESDFDKAMGTDWKNVELRSYEFANEDGENASLVETKDSRESRRGSEIPLTKEERLNLRATAKKTKDGDFHPVQAVQAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRC7</accession>
    <name>A0A8H6CRC7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156086.1">
        <property type="nucleotide sequence ID" value="XM_037298750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRC7"/>
    <dbReference type="GeneID" id="59336279"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013945">
        <property type="entry name" value="Pkr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251:SF1">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08636">
        <property type="entry name" value="Pkr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="167" mass="18200" checksum="15D03796EAAB6308" modified="2022-01-19" version="1">MATFFTDLISSIFTPGPTPSIIIATNASFAALQVVLLVLLILTYSVHFVVLSFLCAGLWWAINWFVTELEAANKQERDMKQSREMKRGQGQEGMGVEDSGTETEGGGTQSEGGVSGSMSERKTERLLRPEDAQGVLRKRMSSGEASSGDLSTDSDWDKVSEAEEVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7I1</accession>
    <name>A0A8H6F7I1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147271.1">
        <property type="nucleotide sequence ID" value="XM_037297146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7I1"/>
    <dbReference type="GeneID" id="59334648"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="101" mass="11527" checksum="F2E610880432A668" modified="2022-01-19" version="1">MIKMTKVRQDRTSLKTIHCSLLEVDGHNVLYIPNPTGMGGTRVLNMPTKETIVDEYSLHDAAKKDDKDHDNQTEEPHGTLKVIKERSQKRKCQQEEVTPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7T2</accession>
    <name>A0A8H6F7T2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147269.1">
        <property type="nucleotide sequence ID" value="XM_037297144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7T2"/>
    <dbReference type="GeneID" id="59334646"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034873212" evidence="1">
        <location>
            <begin position="23"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="482" mass="52459" checksum="643E69D03AC467B2" modified="2022-01-19" version="1">MDERSWLLLLYATLTCILTSDASPVTSSTYPILSKRTVGNCTDLRQNLTQTCWEELGMATFLTDWWQTNQAACSGANTSFVSCYQQSVGVEEQQCDSTGPNMCDYPENFTGYTPQQAYTLYTIFAIWQWFESIYEAIENADLSASGPVGKIVKTINPEVQQEQSLGDFLQALTAFAPLLTLPAELGKVITSVTETAMRQSPGVLKQLNPTGTLDSEVVQISNIYDGLSTIKTTYQQNISNALALVQQDFTSFNLFAANGSFIATRASLEAETTNLTKALETYIVSSCLTASNIIITLARETNPQELASNGSMTTTDLIQCGSYDQYGVCSAWWYDPDTNAAFGLSSLRNMEINYYDLMETLFSNGWTTGADLFLGAKACADYMAVYGGANDPTLDPVTLVPRCISNVQVCVWDQSCAPLDKDCAFTGEYGWNLCKPQYGYEADDCGGNGIPSEDVPAAYLGPLTTITAEEGGSDLIVCHGNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7V0</accession>
    <name>A0A8H6F7V0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">O-methyltransferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005846</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147932.1">
        <property type="nucleotide sequence ID" value="XM_037296751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7V0"/>
    <dbReference type="GeneID" id="59334251"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF1">
        <property type="entry name" value="HYPOTHETICAL O-METHYLTRANSFERASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="29"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="268" mass="30296" checksum="2A53E3CBF8296CB4" modified="2022-01-19" version="1">MDDMLHVCSRLPEYFKSHPLESPSSPTHNPYSWTYGMEGQDWIKVVGRSPEKLRKFAIGISGKWSTVPAVGIYPFDTELAPLSATCQRQNRVLVVDVGASQGGTMKEIRQAFPRLTGRIITQDLARVVDHVPAGYLPPELDIHPTVHDFWTPQPVRNAQDPLFDEKISSDDESGLADRDVVCRTILRHNVDAMGPDSRLLIAEVLVPDRVQKEELYCYWMDLTVLTFAGRERSEADWRDLFESVGLALVKVWRAEVGTQAVVEGRLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8C9</accession>
    <name>A0A8H6F8C9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005504</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148395.1">
        <property type="nucleotide sequence ID" value="XM_037296413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8C9"/>
    <dbReference type="GeneID" id="59333910"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051164">
        <property type="entry name" value="NmrA-like_oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748:SF5">
        <property type="entry name" value="NITROGEN METABOLITE REPRESSION PROTEIN NMRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748">
        <property type="entry name" value="NITROGEN METABOLITE REPRESSION PROTEIN NMRA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="3"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="347" mass="38307" checksum="578EEAE321CD787F" modified="2022-01-19" version="1">MTNKTLVIVNATGRQAASLARVASAVGYQVRAHVSQKTTPVGQELEALQNVTLIQGSLEDKELVADLFAGARKAFINTISWGDEVAIGKSLADAAKKAHVQQYVYSSMPDHSVFGKGWPAVPLWSCKFTVENYIRQIGLPATFVYTGIYNNNFTSLPMPLFRMALQSDGSFEWKAPFDPHIAIPWFDAEHDTGPAVLQILKDGPQKWSGHRIALAFELLTPTRVCRLFSCALRRPVRYVCGQTIDIEVSVPNGYREQLLALEVLFGKYKAPYFGMDLERGVSREVNDANGWNGDGSGGDSGKGTAVEEARALWPGWRGIEEYAREVFVCAHERHLGGRSEADEDISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8J6</accession>
    <name>A0A8H6F8J6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148153.1">
        <property type="nucleotide sequence ID" value="XM_037296171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8J6"/>
    <dbReference type="GeneID" id="59333666"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="570" mass="62528" checksum="7E04E16B850DEFB4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8P0</accession>
    <name>A0A8H6F8P0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">sterol 3beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.173</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Autophagy-related protein 26</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_005115</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22724"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15889"/>
            <dbReference type="ChEBI" id="CHEBI:37424"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.173"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:22725"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D-glucoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61836"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16933"/>
            <dbReference type="ChEBI" id="CHEBI:52973"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:61837"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.173" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148725.1">
        <property type="nucleotide sequence ID" value="XM_037296027.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333521"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016906">
        <property type="term" value="F:sterol 3-beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13215">
        <property type="entry name" value="PH-GRAM1_AGT26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13216">
        <property type="entry name" value="PH-GRAM2_AGT26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000303">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000029">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000009">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase UGT80A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048066">
        <property type="entry name" value="ATG26_PH_GRAM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048065">
        <property type="entry name" value="ATG26_PH_GRAM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010610">
        <property type="entry name" value="EryCIII-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050426">
        <property type="entry name" value="Glycosyltransferase_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050:SF25">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06722">
        <property type="entry name" value="EryCIII-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="264"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="64"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="439"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="439"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="556"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="586"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="618"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="662"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6219290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1422" mass="159044" checksum="AEE3BECF50400091" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8S2</accession>
    <name>A0A8H6F8S2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148922.1">
        <property type="nucleotide sequence ID" value="XM_037294875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8S2"/>
    <dbReference type="GeneID" id="59332364"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="13272" checksum="826B3009B8C5B6EB" modified="2022-01-19" version="1">MRLDTVSYLDSLREATETNITSSRYSITEVSIYTCNIAEKEAPESEDSSAIYEKANAEKTAEKTTEKTMEDTAEKSSGNKATIVKNTAQLSTIPRQIITQQAENTTATWPIISACLGNITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8Y0</accession>
    <name>A0A8H6F8Y0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005533</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3A3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148424.1">
        <property type="nucleotide sequence ID" value="XM_037296442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8Y0"/>
    <dbReference type="GeneID" id="59333939"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031774">
        <property type="entry name" value="SF3A3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024598">
        <property type="entry name" value="SF3a60/Prp9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021966">
        <property type="entry name" value="SF3a60_bindingd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF2">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16837">
        <property type="entry name" value="SF3A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12108">
        <property type="entry name" value="SF3a60_bindingd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11931">
        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="9">
        <location>
            <begin position="411"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="274"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6218989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="506" mass="58571" checksum="AC75578FFD55FB94" modified="2022-01-19" version="1">MLLEEQRFLHEDLERLEQGISDRVAEDPRHVRDRLNRDHQISGFLNRIQDQSKRLLDIYKDTEGLRSKEIQSISTGDPFDEFYKQMTEIKEHHRRYPNQPVENLERAYKRKASGEGESATAEVDNMFTGEEAFGRFLDLTTLHEEYLNIQSKRVSYLQYLQIFDNFGPQYIAMKRAEKSTDQYFNYVGRLADYLESFLRRTRPLEDLDKFFASIDQDFERAWEEDSVPGWGPEAQPAGSAVPVTEGTGEGVWCPDCEKEFKNDNVYKSHLNGKKHIKNAKARQARGETNGTNGGAPNGHANVRGSTVQRLKERAIAEREYRIKRLASTMETVRTDSRVNVERKQGMTERERQMELDAFFAESTEAPQRNGDDDSDSDGDEKIYNPLKLPLAWDGKPIPYWLYKLHGLGVEFPCEICGNFVYMGRRAFDKHFNEARHIYGLKCLGITNTTLFREITGIEDALKLWDKIQRDKKKEKSGGENVVQMEDADGNVMPEKVYYDLQKQGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9K8</accession>
    <name>A0A8H6F9K8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OsmC-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003866</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149476.1">
        <property type="nucleotide sequence ID" value="XM_037294788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9K8"/>
    <dbReference type="GeneID" id="59332277"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052924">
        <property type="entry name" value="OsmC/Ohr_hydroprdx_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35368">
        <property type="entry name" value="HYDROPEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35368:SF1">
        <property type="entry name" value="HYDROPEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="OsmC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="19138" checksum="57AF64335BDDA830" modified="2022-01-19" version="1">MDSTTLRTKQAPLKERYKTDPSSALLTLKTTGTLSSTSIACSLSSGPALAAAKSRTAGLHPAAGGDDPEISGELCSGDMLLEALVACAGVTLKAVATAMGIDIAEGEVRAEGDLDFRGTMGVDRGAPVGFVGIRLEFVLDTDAEPSKVEKLVQLTERYCVVLQTISHKPKISVTSSKKGLVTAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9Y0</accession>
    <name>A0A8H6F9Y0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149821.1">
        <property type="nucleotide sequence ID" value="XM_037294440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9Y0"/>
    <dbReference type="GeneID" id="59331929"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="56"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="356" mass="39985" checksum="AB88B27DB7CFBBAB" modified="2022-01-19" version="1">MASTIVASTLRALTYSRGDICDRTINTEYLQNDCPLDQTSANAHLACRIQPATASLGRLDILPVETIQIIFSNTDLSTLTLLRSVSRRASCLVDSVPAYKKVVTHSPNALRALISTHTAQYFTANDVFDALLAHECFLCGRFGAFLYLLECRRCCWDCLISVEDLLPICKASAQTLYRFDAQTMDEMPTVLTIPGSYGLRHKQSTHRQPKGEKRVAMVAYGAARKAGLNVQMGKKLTDHYEKAKLETRVALRGPVAQDPMRDMDCMNYDHAGYKAGFGLEPQRFMAAVRFPTLITGTDKIEWGFSCLGCLERAEYGDEERFWNEQYTTEGMVRHLDKCQRAGQSWINDRVTEVHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA66</accession>
    <name>A0A8H6FA66_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002590</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.3" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150345.1">
        <property type="nucleotide sequence ID" value="XM_037293516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA66"/>
    <dbReference type="GeneID" id="59331003"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051730">
        <property type="term" value="F:GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001690">
        <property type="entry name" value="tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019039">
        <property type="entry name" value="T4-Rnl1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012387">
        <property type="entry name" value="Trl1_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015966">
        <property type="entry name" value="tRNA_lig_kin_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015965">
        <property type="entry name" value="tRNA_lig_PDEase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004:SF1">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09511">
        <property type="entry name" value="RNA_lig_T4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08302">
        <property type="entry name" value="tRNA_lig_CPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08303">
        <property type="entry name" value="tRNA_lig_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019634">
        <property type="entry name" value="tRNA_lig_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="T4 RNA ligase 1-like N-terminal" evidence="6">
        <location>
            <begin position="73"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase kinase" evidence="5">
        <location>
            <begin position="401"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase phosphodiesterase" evidence="4">
        <location>
            <begin position="565"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="613"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="613"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="2">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR019634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR019634-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="818" mass="92019" checksum="93EE84DE69F8D4E3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAF7</accession>
    <name>A0A8H6FAF7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002561</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150316.1">
        <property type="nucleotide sequence ID" value="XM_037293487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAF7"/>
    <dbReference type="GeneID" id="59330974"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="256"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="289"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="187"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="302"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="17"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="328"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="415" mass="44310" checksum="48BE9DCC399796CB" modified="2022-01-19" version="1">MYGEDSSGRRVSLLNHSSSNTTTTTQNTFSQPKIPNRSRISISSVSSCTSRDGYNSSPSTSSSGSPRHTRSPPTPQLVRFDSQSSLSQSTPSPMTPSNGFDLMDGQHPKNKNYNDLLNGNGNSLPYYTNPAFGRSNPNGYPTLHEASQQPYYNLDSQQPLPSLNGYPDGLFHHRGTLQPLQTQLQHPYSTNTSPILPTPSSATSTNTPSHSLPPISATIKTGPNTPITTSLTASTTATANGQNASATKNPPPKKKYPCPHAVRHNCTDTFTTSGHAARHGKKHTGEKNILCPTCKKAFTRKDNMKQHERTHRHSNSKNDSVAGSPVIGSAKGSRRASSSAGADAMDVDSDGSGMGMEPPRAGRVARPKMQRSELSEIMEGLDRENGMHKDEDADADGEGESPGLDALATAASELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAJ3</accession>
    <name>A0A8H6FAJ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase family 8 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150735.1">
        <property type="nucleotide sequence ID" value="XM_037293082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAJ3"/>
    <dbReference type="GeneID" id="59330569"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000171">
        <property type="entry name" value="Glycosyl transferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="35869" checksum="F94FBD93C0E7E647" modified="2022-01-19" version="1">MANSTNPDTTRVVDSKKVWTTLITNTAYLSGLLTLDYSLKKHGSEYPLLALYTDTFPPEGHRALEIRKIPKKRVKYLLPAVHKDFTNDPRFYDCWSKLTPFSLEEYDRVVQLDSDMMVMRNMDELMEIELDAPESEGKGNRVFAASHACVCNPKKKPHYPKDWTPENCAYTSQHDHPDAAQTQGASSTSGLGIPNGGLQVVNPSKTLYDLIQERMASDSAVMNYEFADQSLLSDLFSGRWVALPYTYNALKTSRETHAPIWRDDEVKNMHYLLSPKPWDEKLGEESNPTHRWWTEANMERLREEKQRGIDDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAR0</accession>
    <name>A0A8H6FAR0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150930.1">
        <property type="nucleotide sequence ID" value="XM_037293277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAR0"/>
    <dbReference type="GeneID" id="59330764"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="1">
        <location>
            <begin position="18"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="247" mass="27971" checksum="A3F7FA7FCD368140" modified="2022-01-19" version="1">MARIKKSVVTAQKPFPILSLPPEIRNHIWRYAVVKDENVTVRPHGRQESIQGLVPSCLRSGRELQRHREDDERRTNSTPLALAFTSRQLYLEVTPIYYSENSFYFPNSWYCISAESTLENFTAAIGPQNASNITVAYFDTLACPLNQYLSVLTGLKQLTVYPPPCAFVSTPYITLWRSQMSRYAQSHASVVIKHLENMEGARLSFLCKTPRDSRGMTWKVALAMPAPRATVLASFKTARSPKLCQPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAY6</accession>
    <name>A0A8H6FAY6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar-sorting protein SNF8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001780</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the endosomal sorting complex required for transport II (ESCRT-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SNF8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151247.1">
        <property type="nucleotide sequence ID" value="XM_037292708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAY6"/>
    <dbReference type="GeneID" id="59330194"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000085">
        <property type="entry name" value="Vacuolar-sorting protein SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000397">
        <property type="entry name" value="Vacuolar-sorting protein SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016689">
        <property type="entry name" value="ESCRT-2_cplx_Snf8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806">
        <property type="entry name" value="EAP30 SUBUNIT OF ELL COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806:SF0">
        <property type="entry name" value="VACUOLAR-SORTING PROTEIN SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017215">
        <property type="entry name" value="ESCRT2_Vps22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="260" mass="27937" checksum="382222A598D91167" modified="2022-01-19" version="1">MSARRGVGLSAFQNRSSLSTSYATHGASLRSTHLSSLQTQLSVFQSLLHNFALQHADTIKSNPSFRAEFARMCNAIGVDPLAGSNVKGKGAGKGAIGSLWAKVLGGDVNDFYFELAVRVVEVCRGSRGGNGGLLGVEECKEQVKKGRGIGGGMDVSDDDILRAVKSLNPLGSGFSIVTVGSKQMIRSIPKELNTDQSRVLEAIQVLGYVTASMLEDNLGWEHARAETVIEDLLADSLVWVDLQAPEKEYWSPTSMNDFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB04</accession>
    <name>A0A8H6FB04_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150916.1">
        <property type="nucleotide sequence ID" value="XM_037293263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB04"/>
    <dbReference type="GeneID" id="59330750"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="647"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="855" mass="91663" checksum="1D8C9399791A7DA5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB22</accession>
    <name>A0A8H6FB22_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150748.1">
        <property type="nucleotide sequence ID" value="XM_037293095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB22"/>
    <dbReference type="GeneID" id="59330582"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="38674" checksum="BC4CB94142EC6425" modified="2022-01-19" version="1">MADPSLTPAQATKIDADPAGDLVLVIGSGDNQRSIRASSKVLSLASPVLAAMFSPRRFLEGTSLSSSNPPEIYLPEDDPEAVTKFCHIVHFREYHGKQPGPSFNQLRDMAPFCDKYDAGLAFNPWSELWLHARLGSETPGGYGTLLALAYAFDNQENFWTSSRNMIQYDTVERSRDTIDELFTLLPQGLYGKLAVDPLSWQNPDLFSTNYPSFYKASIDQDRKSGLLDLSSAVEHIMARFFQDSPRNLLSEIIQVRAGYVFKELYRLNIWPISTRVESSNLDRIQRAITSFIEQENAAFKQQLITVVDRAVDAQKGLCLWCVRKGNIHSHQGNCHARLRYLCTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB94</accession>
    <name>A0A8H6FB94_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Plasma membrane channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001719</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151186.1">
        <property type="nucleotide sequence ID" value="XM_037292647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB94"/>
    <dbReference type="GeneID" id="59330133"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049456">
        <property type="entry name" value="Anoctamin_N_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20877">
        <property type="entry name" value="Anoctamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="606"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin alpha-beta plait" evidence="8">
        <location>
            <begin position="13"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="171"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="663"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="668"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="726" mass="82840" checksum="96B3BE03A4C02C31" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBC6</accession>
    <name>A0A8H6FBC6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151622.1">
        <property type="nucleotide sequence ID" value="XM_037292203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBC6"/>
    <dbReference type="GeneID" id="59329689"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="171" mass="19559" checksum="15E5DC7DA533A351" modified="2022-01-19" version="1">MAFCPGERGLYVLQAQCTDVRLLGQAARQEWHSEARDLPRITSMLDIDPEKTAVRSQDCFTTRVKRLAKVFPRPLYTTFHVHFYEHDAANTFDEVAEGKPSSPVPPRQRSIPISLGMPRWRTKPPKPAHTATATVTTARIPSETRLSEPYEPDVRPNSRKLRMELRGHKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBM6</accession>
    <name>A0A8H6FBM6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151304.1">
        <property type="nucleotide sequence ID" value="XM_037292765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBM6"/>
    <dbReference type="GeneID" id="59330251"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="624" mass="69837" checksum="839A394E31EC1D5D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC32</accession>
    <name>A0A8H6FC32_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">BAG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000683</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sterol O-acyltransferase that catalyzes the formation of stery esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151982.1">
        <property type="nucleotide sequence ID" value="XM_037291620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC32"/>
    <dbReference type="GeneID" id="59329101"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034737">
        <property type="term" value="F:ergosterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008204">
        <property type="term" value="P:ergosterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF9">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="398"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="12">
        <location>
            <begin position="835"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="707"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="738"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="752"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="795"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6222636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="907" mass="102140" checksum="A54FBC7EDB2CE31F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCG0</accession>
    <name>A0A8H6FCG0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152544.1">
        <property type="nucleotide sequence ID" value="XM_037291109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCG0"/>
    <dbReference type="GeneID" id="59328588"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF2">
        <property type="entry name" value="AMIDASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="3">
        <location>
            <begin position="74"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="3">
        <location>
            <begin position="189"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="503" mass="56511" checksum="27B83E6E7E5C20A6" modified="2022-01-19" version="1">MSWQSNAQGKRASLIELIPLKWRIPTSDIPSVTRLRDFGEYICRFLDRRELEITNAPSSRILANIRSGEWTSVDVTRAFCHRASVAHQLTNCLSEICFVAAEQRAKALDESFLRTGQTVGPLHGLPVSLKDRFNIDGLESACGYVSWLGKEKSENDEGILVKRLRRMGAVFFVKTNVPMSMLLPGLPVNGSVVGPMAGDLPTIEMAMKVVLDTFPWQDDCDVLEMPWREEKLQNIRSRTARHRERDGKLVFAVMSCDGNVQVHPPVQRAMALVTKALLQRGYEVIEWQPPPHHDAVQTLVRQHTADSTQGWFQILGSTAGKEVRNAIDASGEPPVHQLQTWYDNDNVEPSSSAEFWDLCNQRDQYRSEYNQYWTSTREKNVAKRQVDGVIMPVAPTAAVEEGCFDYYAYSGIVNLLDYTSGSFPVTFADRSIDVETPDHVPLNPTDRAVWRTYQKDLFDGAPVGLQVMGKRFEEEKVLGLMEALTAALQDFSVGQIGEPSSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCN7</accession>
    <name>A0A8H6FCN7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152799.1">
        <property type="nucleotide sequence ID" value="XM_037291364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCN7"/>
    <dbReference type="GeneID" id="59328844"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039258">
        <property type="entry name" value="ZNF511"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21354">
        <property type="entry name" value="ZINC FINGER PROTEIN 511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21354:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="230" mass="25597" checksum="A86124701666FDB1" modified="2022-01-19" version="1">MPKRSREDSPSPTTTFSLSPTPDPSCPASTDAPVHPSKYIQTSDERSTRRVMKCSLPPHHDTISFSTFEEFELHYAREHAHRCSECRKNFPTEHFLGLHISENHDPLTEARRANEEKTNYDFQIVNTGIDKRSSMLRSRPRKHSSAASRAFHREQRTNSTEAFQGHNPDSANTLESSAAETSKGSSGRLGHSPSLPADVDELVSTMSALKFVPPSVHFGRGGRRGGLSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCQ4</accession>
    <name>A0A8H6FCQ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hexosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001106</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152400.1">
        <property type="nucleotide sequence ID" value="XM_037292038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCQ4"/>
    <dbReference type="GeneID" id="59329524"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6223054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="253" mass="28999" checksum="6B00394FAC3893F8" modified="2022-01-19" version="1">MSAYFSQQRRNVIRATWQTLYPNPAFETCFFIAKPPPLWQSLIKQENDTYGDLILLDHLEESTKVALFIKTVEFLKHLLGSGKPSWKFVSKIDDDSFVDAERFYHEFLRPRIDRHAAAADQNGTLIARRLRLEHSDRNFSTPGGQFYTLSWDLVRILVETQARKLITNVPEDVLVGRLLYDADAKFEFVDLDDTRAFDVTDGLTRLSQEREDLGPMDAAAINPHKMKDDETYLRVAELFGKDGFVGHPSGAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCZ4</accession>
    <name>A0A8H6FCZ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Glycerol-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="4 13">1.1.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_010516</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a quinone + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:18977"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="4 13"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153002.1">
        <property type="nucleotide sequence ID" value="XM_037301368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCZ4"/>
    <dbReference type="GeneID" id="59338906"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.870:FF:000001">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000001">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.870">
        <property type="entry name" value="Alpha-glycerophosphate oxidase, cap domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031656">
        <property type="entry name" value="DAO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038299">
        <property type="entry name" value="DAO_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985:SF15">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16901">
        <property type="entry name" value="DAO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00977">
        <property type="entry name" value="FAD_G3PDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00978">
        <property type="entry name" value="FAD_G3PDH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="5 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="16">
        <location>
            <begin position="108"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-glycerophosphate oxidase C-terminal" evidence="17">
        <location>
            <begin position="522"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="676"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="676"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6223942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="725" mass="79926" checksum="7ACCCDD42974C448" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD10</accession>
    <name>A0A8H6FD10_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000409</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152783.1">
        <property type="nucleotide sequence ID" value="XM_037291348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD10"/>
    <dbReference type="GeneID" id="59328828"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08276">
        <property type="entry name" value="MDR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052711">
        <property type="entry name" value="Zinc_ADH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45033">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45033:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C1773.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="20"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="356" mass="37914" checksum="9D057D6254A7AD84" modified="2022-01-19" version="1">MPQQWQVHQRITNASRQSPEALVNLELRDVPKPVPGPKETLVRIHAVALNARDLLVLTSSPLYPVPTSAGLVPCSDAAGIVEELGTDSVWKAGDRVILHPNTWLNGRDERGFDITKAFGAGTVAGTLTEYMVIGDERLIKVPEHLSLEEGSVLPTAGSTACHSLFFVPEQPVGDGTWVLVQGTGGVSTIAIQLAAAAGATVIATSSSDEKLALSKQLGAHHTINYRHTPNWEEEVMKMTDGKGVDFVLDVGGAGTIEHSLGSLRQGGIVSIVGILTPAKEIDIVPAILYGAKTVRGQLGSSKTMSEELVRRVDADKIRPVIAKTFEWREAKDAFELLMNQSEVGKIVVKGVTAGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD13</accession>
    <name>A0A8H6FD13_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Telomerase activating protein Est1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000424</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152798.1">
        <property type="nucleotide sequence ID" value="XM_037291363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD13"/>
    <dbReference type="GeneID" id="59328843"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF36">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomerase activating protein Est1-like N-terminal" evidence="3">
        <location>
            <begin position="60"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA-binding" evidence="2">
        <location>
            <begin position="189"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="804"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="715"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="832" mass="92938" checksum="613A9B54819B7301" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD62</accession>
    <name>A0A8H6FD62_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000362</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152736.1">
        <property type="nucleotide sequence ID" value="XM_037291301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD62"/>
    <dbReference type="GeneID" id="59328781"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Dyskerin-like" evidence="8">
        <location>
            <begin position="303"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="362" mass="39778" checksum="2A3C9DB312E262D8" modified="2022-01-19" version="1">MEAYRAAPPVSRTLTALTLGTSLLVYGNIISGSRVVFYLPWIFKFPLPEVWRFVTSFWVTGGGLSILFDTYFLWTYSSGLEKESGRFSQPGDFFTYIIFLGLVILATAGFILGGAVFTQALILGIAYTYSQDNRGKKVSFFIITFNVIWLPWAMLLMTFIMAGPDAALKQGAGLLAAHLYDFLTRLYPTFGGGKNFIRTPAIVKRWFGADKSSSQARGYGTAFRPATQAPGRGTSSGFGFSSAWGTRGQDNSNFYEPQKDERATAIVSITCQRQSIRKMADVPAKGQEVDYTIKPEATTPAVDTSDRPLLLKDDNRLLVRTGHFTPIPMGCTPLKRDLKSFISSGVINLAKPSNPSSHEVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD97</accession>
    <name>A0A8H6FD97_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010679</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153165.1">
        <property type="nucleotide sequence ID" value="XM_037301531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD97"/>
    <dbReference type="GeneID" id="59339069"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052413">
        <property type="entry name" value="SUR7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019:SF2">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="282" mass="31047" checksum="F2683496A0045EC6" modified="2022-01-19" version="1">MALPAFVSNHRSRKVPQEKSTAHISPSSSGLSPNNDALHPSPSTVRAATRTRLIFSLLTSLLFLIALVFLILVEVGNTNAHRAVVGSIYFLKLDLSHIIPQAVPQATLINSIARTLGLHDFYQVGLWNYCEGYGSGITDCSKPQTLYWFNPVEIILNQLLAGATTSHWMFALFLTGVSTCFVSIFLTPLSIYTRWATFPIAIFAFLSALTTTAASIIATVMFIIFEKVIHSAEGEVNIVPEIGVKMFAFMLVLLLCQQARREARKEDWAGKSVERARRSAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDD4</accession>
    <name>A0A8H6FDD4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010800</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153285.1">
        <property type="nucleotide sequence ID" value="XM_037301651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDD4"/>
    <dbReference type="GeneID" id="59339190"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1340.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1340.10">
        <property type="entry name" value="RNA polymerase, Rpb5, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005571">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036710">
        <property type="entry name" value="RNA_pol_Rpb5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03871">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53036">
        <property type="entry name" value="Eukaryotic RPB5 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb5 N-terminal" evidence="7">
        <location>
            <begin position="16"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="6">
        <location>
            <begin position="164"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="237" mass="27118" checksum="EAD7E2B991864A2C" modified="2022-01-19" version="1">MDNQDAERAKIAADADRELTRLWRAWRTIHQMCQDRGYEIGEEEVKISLEDFRKNYADPSGAPDRKKMNTSFRPTDAMIAKYTALPTKAEPNPQPQIGTIWIEFSSDASIGIKQMRAFAHHVNQHKFYTGILVTQASITPAALKIIPVVLPSIIETFQEQDLLVNITHHELVPTHELLSAEQKVELLKRYRLKESQLPRIQVGDPVAKYLGLRKGQVVKIIRRSETAGRYASYRWAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE43</accession>
    <name>A0A8H6FE43_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153994.1">
        <property type="nucleotide sequence ID" value="XM_037301177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE43"/>
    <dbReference type="GeneID" id="59338714"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="465" mass="51611" checksum="08915550C8FD940E" modified="2022-01-19" version="1">MSTLLLPITAPDIFDPTMIQAAARTALLTTTIMLSSYAEMEDAVGNEEWERINQPEVRKRVQNRLSQRRHSEYPTVSSSSPRLHAVAEYSTQGDKLRQQREPVSHEIIIPPSFTSSYTMEGAPGTSDYPSPSLSFNGQDDRHNLPRPNQPQEGYDAFDAFNAQDMLPPDHTVSTTMAQPAQVGSVGMNRSMTPHMGRPAMNNSYQPNNMQPLGSLASGPPTLPAVSRNEYWDSQSLSDPQWAIPSFHQNSVPASNLPAGTNNPRHTSSHMPRPMVRPMPRRVSNASSNQQTNILPTPRASDSHSSPVLQRNIANQAYPRSSSHLENANTESESTRKHNDACSHCRTCRAPSSFDDQKPGQASPKTPTSTPSRDSSQTNSPDHLEILANCSKVLANLARQSDYRDENEPEERPRSSKRSKTNLDDYYVDVNRTLQEDDYGDGHHRHNVIGKVVILCVKNGRKTKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEK7</accession>
    <name>A0A8H6FEK7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pinin/SDK/MemA protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153856.1">
        <property type="nucleotide sequence ID" value="XM_037301039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEK7"/>
    <dbReference type="GeneID" id="59338576"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006786">
        <property type="entry name" value="Pinin_SDK_MemA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04696">
        <property type="entry name" value="Pinin_SDK_memA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pinin/SDK/MemA protein" evidence="2">
        <location>
            <begin position="2"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="146" mass="16123" checksum="35232E0D86CA44AE" modified="2022-01-19" version="1">MLARAHFLRTRAEPMLYYKPWELSPGDEEKIKAQVEEAEEFIEREAKQWDIDNAVTSDEAPPPTNDGADPSETKEEDARAVDTVGAATNGEQHSSPQISEDTNMNDTVASMESGDRETPAEPPEAVKDLGDNGEEVLEGEEDTVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFD3</accession>
    <name>A0A8H6FFD3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phenol hydroxylase-like C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008951</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154639.1">
        <property type="nucleotide sequence ID" value="XM_037299813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFD3"/>
    <dbReference type="GeneID" id="59337346"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012941">
        <property type="entry name" value="Phe_hydrox_C_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038220">
        <property type="entry name" value="PHOX_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07976">
        <property type="entry name" value="Phe_hydrox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phenol hydroxylase-like C-terminal dimerisation" evidence="3">
        <location>
            <begin position="119"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="254" mass="27593" checksum="D3A24FDB73107382" modified="2022-01-19" version="1">MALWLHPEIDAWSTPDVDAEPPSITHKPSGAENQSAYPVTVRTVQVGTETSSYPVAKPTSNGAIEIDGPEPKEEAQCYPRGASNEGGHGGQICDQLRWRTKLDKIAGCGLRTQGSETDIKSQQHLAPNTPLGQHFLSFVVINHCDARSWHFGELLKAGGLFAVVLFAGGVSRTDQIQRVHKFAGALANVVIPLVQHHISGGGQKAIYDVADILTIRSAPGGQVEYSRNCYVPSMRSLVVTYCVYRRSKVSFIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFG4</accession>
    <name>A0A8H6FFG4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009907</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154613.1">
        <property type="nucleotide sequence ID" value="XM_037300765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFG4"/>
    <dbReference type="GeneID" id="59338301"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="188" mass="20351" checksum="5D53B086E9105886" modified="2022-01-19" version="1">MESWLNTWNSTTTTCEPGERWANCFMRQAGVTTNTTTSIRCDLIGPNTCPEPSIDVFESASAEASYGVASIWALQQYMTTLYQFLEGDQGLSLINTGFLNQTAPRSAKTILVNILDEIGDPVATQLTTIIGSPVPIKQGSFIIPTQAQAQQIVGQTVGNLLQWLMTDWLEGGFTALAIEGNLISILGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGR2</accession>
    <name>A0A8H6FGR2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155866.1">
        <property type="nucleotide sequence ID" value="XM_037298530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGR2"/>
    <dbReference type="GeneID" id="59336057"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="393"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="627" mass="66509" checksum="C90549CE4087C4D6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHL2</accession>
    <name>A0A8H6FHL2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6590 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156028.1">
        <property type="nucleotide sequence ID" value="XM_037298692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHL2"/>
    <dbReference type="GeneID" id="59336219"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046497">
        <property type="entry name" value="DUF6590"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20233">
        <property type="entry name" value="DUF6590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6590" evidence="2">
        <location>
            <begin position="143"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="326" mass="37825" checksum="FB454BE9039152B9" modified="2022-01-19" version="1">MTSTATFTQMSTRTDGRSTVHSHSIDHWSPTRDRFSLRHHYSSRDEYSPNKRQHCDSDRDYPREDAALHVIQNRAIARLRSSTASAISRDSIGSYQSRTYNPNNPIDKKSRSRADFEILEIRNYPLPRMKDISATICCRFPKEEFNLGTIIKAPIHEEDFKWTPRPLSVEASETADEQGRYSHSGMSTPRTTPNKRRHITFSNWNDPIYTEMRFMIVVALFYDHYLAIPLYTHGGNGLAHKDNKWEYASIEDYREPRSLQEAGFLLHTEYLKPDCKHLLPKSVAHIAYPVSRKYTLPVAPQGRLREDSKNVLVKLFGQYMLGMKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHT8</accession>
    <name>A0A8H6FHT8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156487.1">
        <property type="nucleotide sequence ID" value="XM_037297852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHT8"/>
    <dbReference type="GeneID" id="59335356"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034318717" description="AB hydrolase-1 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="426" mass="45572" checksum="B59FB94E3B8545F1" modified="2022-01-19" version="1">MFPKSALAVVVLAGSAAAKTCINMTVPVTISARTAVFNITVPQTNLDATTFAQNQTQQGRNFTETVLSGYATTAGTYDISTQFCMPSSMNVTMTKPTVQILTHGIGFDKTYWDLSYNNFNYSYIDVATDVYKYCTLSYDRLGIGNSSHGEPLDEIQASLEMAALFELTMMLRNATFPGIKQTFGKVTHVGHSFGSAQTYALVNMYPTISDGIVLTGFSMNASFVGFFEAGSNFEQAYLNQPFRLGNVSSSAVESVLNTNISSSTVESVLNMYSLTDYVVGLNSSSPSLDYPAGYLTNANIGANQYLFFLPGFFDPGLLLVAENTKQPVTVGELLTLGSPPMMNAFAGPVLIITGSNDLPYCGGNCFATGNASLPSIPAAAAMNFPMVPSGDFTAYIQPNSGHGINLHYNATGAYDVIQTFLQSKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIM8</accession>
    <name>A0A8H6FIM8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribonuclease P protein subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007354</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156880.1">
        <property type="nucleotide sequence ID" value="XM_037298245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIM8"/>
    <dbReference type="GeneID" id="59335753"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027081">
        <property type="entry name" value="RNase_P/MRP_p29_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR027081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="241" mass="27198" checksum="74D9E3EF5CA8FD4E" modified="2022-01-19" version="1">MANNKHIAHDLLTQAHPAPLAASIFVDKVLHKPLHLRPTSPDHTSQDARAKRRIQRLRKKEKSQRRQKVKPLSAKEKRDSGIYEIPDTAKKYEIYVPLHRMWLGYVWEILGMNMDERAFVTANSAGSKLASADYHGAEVTVVRSKCMGMVGLAGIVVRDTKFTFQIVTKMNELKTIPKKHTIFGFEIPQPVSNQSLGQEAATAESKAESASLVFELHGSQFENRAPDRATKKFKQRNTNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIZ4</accession>
    <name>A0A8H6FIZ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-activating enzyme E1 1</fullName>
            <ecNumber evidence="4">6.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_007463</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156989.1">
        <property type="nucleotide sequence ID" value="XM_037298354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIZ4"/>
    <dbReference type="GeneID" id="59335862"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004839">
        <property type="term" value="F:ubiquitin activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01491">
        <property type="entry name" value="Ube1_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01490">
        <property type="entry name" value="Ube1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12550:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000015">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.180:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.60:FF:000002">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.60">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032420">
        <property type="entry name" value="E1_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032418">
        <property type="entry name" value="E1_FCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038252">
        <property type="entry name" value="UBA_E1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018075">
        <property type="entry name" value="UBQ-activ_enz_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01408">
        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16191">
        <property type="entry name" value="E1_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16190">
        <property type="entry name" value="E1_FCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09358">
        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="11">
        <location>
            <begin position="1006"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="884"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6229347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1141" mass="126920" checksum="7CF2AB7E971ECD1C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK04</accession>
    <name>A0A8H6FK04_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sn-1,2-diacylglycerol cholinephosphotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004296</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157215.1">
        <property type="nucleotide sequence ID" value="XM_037295214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK04"/>
    <dbReference type="GeneID" id="59332704"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF77">
        <property type="entry name" value="CDP-ALCOHOL PHOSPHATIDYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034334470" description="Sn-1,2-diacylglycerol cholinephosphotransferase" evidence="8">
        <location>
            <begin position="17"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="341"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6229958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="41154" checksum="82C93D2D50635AE3" modified="2022-01-19" version="1">MVTLLGFFFILTNVICLEIWMPDLVGPGPSWLYYSFAVGLWMYSTMDNVDGKQARRTGQSSGLGELFDHGIDSLNCTLASLCETAALALGPTPKGVFTALIPCLPMFFSTWETYHTHTLYLGYFNGPTEGLILACTFMILSGYYGPQMWHHPISDLVGHSEIIGHNSVIDLWIPIIFGTFLVAHLPACVYNVIKARREQGLPVAPVFLEWTPIAVYTASICAWLYSPYSTLMRENRLVLFCLTMSFVFGRMTTKIILAHLTRQPFPYWTVMLIPLIGGAILGNLPRVGLSPVTAEVELWYLRAYFVFATVVYFRWALLVINSICAYLGINCLTITPKPAPGQDTVGERSVSNGYAPEKHVPNGRAVQKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKB3</accession>
    <name>A0A8H6FKB3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc metallopeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004460</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157378.1">
        <property type="nucleotide sequence ID" value="XM_037295377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKB3"/>
    <dbReference type="GeneID" id="59332868"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013536">
        <property type="entry name" value="WLM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053000">
        <property type="entry name" value="WSS1-like_metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622:SF1">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08325">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51397">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WLM" evidence="7">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="327"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6230121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="48167" checksum="7CE051025918A1E7" modified="2022-01-19" version="1">MAFEHDPAVSEYQHERSRPREAEALQTLRKIASLVKPIMRQRGWKVGVLTEFYPPERNLLGLNINKGQKICLRLRYPGDERQFLPLEEVTDTMLHELCHIVFGPHDESFHNLWNQLRDEYDQLIRKGYTGEGFLSTGHKLGGRGLIPMHEARRLARVNAEKRRTLTAGSGQKLGGAPVRRGVDIRNVIADAAARRAAIMKGCASGVGAEREKEIDKETNKKGFRTKAEEDDANEEAIMIAYIDLVQEEERDKYGDAYIPPSKENPAGSQGGFNRIKSESGIRSNLAPQVPASTKPILPITNTVDLGYGQSTPPPHTQPPSTKPQTPFSDPWTCEICTLVNPANYLCCDACSTERSFPPPLPLSNPQTIGSTQARPSSIRDSNSKKAVKSLMSLEARTSQLPKKPIGWSCHSCGNFMESEWWTCARCGSMKQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKW4</accession>
    <name>A0A8H6FKW4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157639.1">
        <property type="nucleotide sequence ID" value="XM_037295638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKW4"/>
    <dbReference type="GeneID" id="59333130"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="595"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="625"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1084" mass="111247" checksum="3446A0C809BB3700" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL34</accession>
    <name>A0A8H6FL34_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157798.1">
        <property type="nucleotide sequence ID" value="XM_037295797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL34"/>
    <dbReference type="GeneID" id="59333291"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="296" mass="32899" checksum="2CC22D24E5E153C7" modified="2022-01-19" version="1">MTTEFNPLEPHLELSPPASYSLQQQCMAFGLLLLCYLSLASLTALVPAPNTSTPRLPTGEIITSESLKNSTPPSLSNSSALVDNFLLPYRFKVPDTDILLRLGFGIRRSLDRNHMRSLIAVAEEYIDEQIAALPPATPDFQHVYPVAQGRNQTFYKSLGDGIRLVINDHHQIGKLFTWDELEDVTGFRFYVEDFAPDITTRCMGYGSLGPEEYASDDEMDAELAAEERRWCACYSRGPLAIPPSLGLGVKLDEADDEIDVELAAETKRWACPRRATSYCKAIALIGREDPNHRSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYI3</accession>
    <name>A0A8H6KYI3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_007301</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156827.1">
        <property type="nucleotide sequence ID" value="XM_037298192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYI3"/>
    <dbReference type="GeneID" id="59335700"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19494">
        <property type="entry name" value="Elp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008728">
        <property type="entry name" value="Elongator_complex_protein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896">
        <property type="entry name" value="PAX6 NEIGHBOR PROTEIN PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05625">
        <property type="entry name" value="PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="370"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6229185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="395" mass="41443" checksum="8D9F1A8A387BAEA7" modified="2022-01-19" version="1">MSFRKRNVGLAGPSSAPPSPKDSPVTHASVPGVRPSPLDGRPTTSTGTQTLDDLLAGHAGLALGNSLLVEENGTTDFAGALLRYYAAEGVVQGHTVHVVGVGEQWSKELPGLVGGGGAVGKESGSGVGVDQEKMKIAWRYERLGEFGTGTAGSRATPAPNRNPVPSSPGQSDSTVTIPFCHSFDLTKRLSLPTSGTINFTAISPTAENPFASILQPVTLAIASSPPNTIHRLIIPTLLSPALFPPSASHPRHLLPFLHSLRALLRHHSTRLSAMLTLPLSLYPRSTGLVRWIEHLCDGVLELAPFPHSIDVEPPLTTSSGSGKAEEKPQGMLKIHKLPVLTEKGGGGGGGDDLAFSLNRKRFVIKPFSLPPVEGDQEAQRGEAEGGKVGKGDIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZX1</accession>
    <name>A0A8H6KZX1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyltransferase MbtK/IucB-like conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004742</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lysine N-acyltransferase MbtK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157657.1">
        <property type="nucleotide sequence ID" value="XM_037295656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZX1"/>
    <dbReference type="GeneID" id="59333148"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019432">
        <property type="entry name" value="Acyltransferase_MbtK/IucB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31438">
        <property type="entry name" value="LYSINE N-ACYLTRANSFERASE C17G9.06C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31438:SF1">
        <property type="entry name" value="LYSINE N-ACYLTRANSFERASE C17G9.06C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13523">
        <property type="entry name" value="Acetyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01006">
        <property type="entry name" value="AlcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyltransferase MbtK/IucB-like conserved" evidence="3">
        <location>
            <begin position="365"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="535" mass="59885" checksum="D09BFAECB4616CA9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZZ7</accession>
    <name>A0A8H6KZZ7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157685.1">
        <property type="nucleotide sequence ID" value="XM_037295684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZZ7"/>
    <dbReference type="GeneID" id="59333176"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF74">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="213"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="315"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="350"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="605" mass="68042" checksum="0ADDC98693FBDFD3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L0C4</accession>
    <name>A0A8H6L0C4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000021</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157835.1">
        <property type="nucleotide sequence ID" value="XM_037290961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L0C4"/>
    <dbReference type="GeneID" id="59328440"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057684">
        <property type="entry name" value="DUF7924"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040976">
        <property type="entry name" value="Pkinase_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42470:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42470">
        <property type="entry name" value="VAST DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25545">
        <property type="entry name" value="DUF7924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17667">
        <property type="entry name" value="Pkinase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7924" evidence="6">
        <location>
            <begin position="266"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal-type protein kinase" evidence="5">
        <location>
            <begin position="484"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="520" mass="57764" checksum="BF5ABCB15341824E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C578</accession>
    <name>A0A8H6C578_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146902.1">
        <property type="nucleotide sequence ID" value="XM_037297701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C578"/>
    <dbReference type="GeneID" id="59335205"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="705" mass="76755" checksum="33B56045AA74F1F1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6H7</accession>
    <name>A0A8H6C6H7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147308.1">
        <property type="nucleotide sequence ID" value="XM_037297183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6H7"/>
    <dbReference type="GeneID" id="59334686"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034247203" evidence="1">
        <location>
            <begin position="23"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="200" mass="22085" checksum="B99098F06D0811D3" modified="2022-01-19" version="1">MLPPAYLLLLLLLLLATPSTTGILNITDLLPFTKPLPNSLTLNPIPNPFPVPNTDITLDFATPPHLAPLLPQTDVTDCFALAKLHFETLIHEHGDGPIAYSYEMTYGRVTLKMFSENAPMRCITYSEALSVLLGISLKMAREGFEFRRLHVLQSDDGELLGMAVNGRFVMNMLAENGDFGKAFGVLKSKIEMAQVNRGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C778</accession>
    <name>A0A8H6C778_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Choline-sulfatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006183</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147657.1">
        <property type="nucleotide sequence ID" value="XM_037297083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C778"/>
    <dbReference type="GeneID" id="59334585"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015024">
        <property type="term" value="F:glucuronate-2-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16032">
        <property type="entry name" value="choline-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000032">
        <property type="entry name" value="Choline sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017785">
        <property type="entry name" value="Choline-sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025863">
        <property type="entry name" value="Choline_sulf_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051849">
        <property type="entry name" value="GAG-degrading_sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03417">
        <property type="entry name" value="chol_sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46615">
        <property type="entry name" value="ARYLSULFATASE K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46615:SF1">
        <property type="entry name" value="ARYLSULFATASE K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12411">
        <property type="entry name" value="Choline_sulf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="4">
        <location>
            <begin position="53"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Choline sulfatase enzyme C-terminal" evidence="5">
        <location>
            <begin position="502"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="65191" checksum="267E74B571791FA8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7F9</accession>
    <name>A0A8H6C7F9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="40"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="380"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
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            <begin position="559"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="669" mass="74710" checksum="908EAB89A0387AD7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
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            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7N2"/>
    <dbReference type="GeneID" id="59334489"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="232"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="569"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="598"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="570"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="671" mass="74993" checksum="13299778C75C2B72" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7S0</accession>
    <name>A0A8H6C7S0_9LECA</name>
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        <recommendedName>
            <fullName evidence="10">ML-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="11" type="ORF">HO133_005680</name>
    </gene>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147768.1">
        <property type="nucleotide sequence ID" value="XM_037296587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7S0"/>
    <dbReference type="GeneID" id="59334085"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF2">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034334327" description="ML-like domain-containing protein" evidence="9">
        <location>
            <begin position="27"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="565"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="10">
        <location>
            <begin position="28"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="635"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="755" mass="81990" checksum="51D38F66308CDF49" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8T6</accession>
    <name>A0A8H6C8T6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005268</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148161.1">
        <property type="nucleotide sequence ID" value="XM_037296179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8T6"/>
    <dbReference type="GeneID" id="59333674"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044669">
        <property type="entry name" value="YneE/VCCN1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281">
        <property type="entry name" value="UPF0187 PROTEIN YNEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281:SF19">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION CHANNEL-FORMING PROTEIN YNEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25539">
        <property type="entry name" value="Bestrophin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6218726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="477" mass="52950" checksum="A8E200AEF5AD73ED" modified="2022-01-19" version="1">MSATTTLHARPSSSALSTNPPLKTPGITMDPDSSSSSPQSTSLRQPSPSPNQRLRPPTRRHTSTIDDLISKPLDIEKHRKMPYFLRMRGSITPRMIVPLVFVAIWSTIITCISQFWYQLVVSSLLLTVLGFVVGLGISFRTSSAYERYVEGRKYWAQLIQTSRDLARHIWIHAEERHDRSPEAGKADLLAKLTALSLINAFAVSLKRRLRFEPYANYDDLAPLVGNLSTFAGAASDAHAVEEPDRTFLKAIGSYLGLEFLESNPRKHIKRADKDGKKLGNLPLEVLHYLSAYVESIIANGTLPNGVHQGMMMANLASLNEVLAGTERVVNTPLPVAYSIAISQITWTYVIALPFQLWDSLRWVTIPGTVVGAYIILGIAAIGREIEDPFGYDENDLPLDRYCKQIEDEINVISSKEPPKPEDFIATEGNRLMYSLSYGAWNAKSTEDIRATLRAKAKTKPMTSFPPVLAEKGAQDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Y8</accession>
    <name>A0A8H6C8Y8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mRNA 3'-end-processing protein RNA14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005020</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 4">Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Nucleus and/or cytoplasm.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148630.1">
        <property type="nucleotide sequence ID" value="XM_037295932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8Y8"/>
    <dbReference type="GeneID" id="59333426"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045243">
        <property type="entry name" value="Rna14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980:SF0">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980">
        <property type="entry name" value="RNA CLEAVAGE STIMULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Suppressor of forked" evidence="6">
        <location>
            <begin position="251"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="21"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="201"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="646"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="872"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1001"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="662"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="891"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="934"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="954"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1006"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1036"/>
            <end position="1049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1055" mass="117333" checksum="BDCEEC0238F44B82" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C937</accession>
    <name>A0A8H6C937_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lethal giant larvae (Lgl)-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005022</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148632.1">
        <property type="nucleotide sequence ID" value="XM_037295934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C937"/>
    <dbReference type="GeneID" id="59333428"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000848">
        <property type="entry name" value="SNARE-dependent exocytosis protein (Sro7), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013905">
        <property type="entry name" value="Lgl_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF25">
        <property type="entry name" value="TOMOSYN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08596">
        <property type="entry name" value="Lgl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="242"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Lethal giant larvae (Lgl)-like C-terminal" evidence="5">
        <location>
            <begin position="518"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="896"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="904"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="925"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1004" mass="109092" checksum="54A87AFDD1023D0D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C986</accession>
    <name>A0A8H6C986_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation machinery-associated protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMA16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148795.1">
        <property type="nucleotide sequence ID" value="XM_037296097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C986"/>
    <dbReference type="GeneID" id="59333591"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021346">
        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20467" checksum="9FA9E6D44746B2D2" modified="2022-01-19" version="1">MVKMPKSLAKVQKKVAKKKGSSTSLHENSRDAQKLRRAGARSEKLDKLAAARAKANQPHLKRVAYFQGAARAATAPLEAEKLHGLVKGFLGRHDEELAEIKSQRRPGRPSSTREDLLQQFMMTEDQEYNGGFWIPDMQDEGNLRILREWNGQYTALNTFKYVRLSRNGTMRKSSFPPKGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9G9</accession>
    <name>A0A8H6C9G9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148790.1">
        <property type="nucleotide sequence ID" value="XM_037296092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9G9"/>
    <dbReference type="GeneID" id="59333586"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="610" mass="67549" checksum="B49B1E958ACB3B5F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9T1</accession>
    <name>A0A8H6C9T1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149095.1">
        <property type="nucleotide sequence ID" value="XM_037295048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9T1"/>
    <dbReference type="GeneID" id="59332538"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="389" mass="44358" checksum="E710B96E39257367" modified="2022-01-19" version="1">MLNRMSSSARKRESVNMTAPKNAGETFTFSPAPGNLDPHAHTHSDQPPYFDLRKCPSMASVISLRRYLSLTRDLPNEIIDIILDLAEYWPHTSSILDMTTRVYSGHMCYGSYPARPRQKLGRLLSTPESRREWKEDGFLIRTPPLGWRCIGDADLSNRCESKPPSTPPPKAKSKPLIPTPRPPTWLPPRGLHPARRVVFEILSREERLENPHRQGSTFCYANSKTSFDATIDTISFPKTTKKSITRSIDPLLWTAEAATKDCHHHQPSEVCYAIRAKSSRSPHPPSKTSELENHSSGVTKNYTAHNRKSVRTWRYDGAERCYLPTTDRDFGSENATEFVQGLREGDCVSLWARARKCDEHVRRRRRQGEQGRAWCNVVDRVAIHVFWAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA84</accession>
    <name>A0A8H6CA84_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004003</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148969.1">
        <property type="nucleotide sequence ID" value="XM_037294922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA84"/>
    <dbReference type="GeneID" id="59332412"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031303">
        <property type="entry name" value="C5_meth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF52">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00095">
        <property type="entry name" value="C5_MTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="32"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="140"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="572"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="618"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="676"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="707"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="726"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="749"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="823"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1212" mass="134433" checksum="3EDDBD8AFAD755FF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAF8</accession>
    <name>A0A8H6CAF8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149104.1">
        <property type="nucleotide sequence ID" value="XM_037295057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAF8"/>
    <dbReference type="GeneID" id="59332547"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="283" mass="33221" checksum="E76CF2F2055017E2" modified="2022-01-19" version="1">MESTPQFPRLRSLKLQTVLFSDYSMLDALLEGDLRELEVDPGHDSLYSEFFEQRGTMPALTTLVWDGKIKADQSLSFLQANTQLSKLSFQWEAPGVFLETRLLPLLTKSFRHLTSLSLKWEGDSIPGSALEAISSLEILQQLHLSAGHQHGWRYSWFIDHALMRSRLKKLTSLRRMAFTRDIYDNEVEWSAPERYYEDRFHNDLDLDADEDEWETWHCESMLSEARNYAMEIPDLEWVYFGQLYMRVSRKDESQGGGAHAFALSKERDQWWTYLREMFGWKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAM8</accession>
    <name>A0A8H6CAM8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003827</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149437.1">
        <property type="nucleotide sequence ID" value="XM_037294749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAM8"/>
    <dbReference type="GeneID" id="59332238"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048244">
        <property type="term" value="F:phytanoyl-CoA dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047128">
        <property type="entry name" value="PhyH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21308">
        <property type="entry name" value="PHYTANOYL-COA ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21308:SF8">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_2G09620)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="408" mass="44965" checksum="E20A1601EF2C1920" modified="2022-01-19" version="1">MRVFQPKGQEMGEAAARPVAEPRLLSHRLFALEKKPSLSALKEYCTQVVDPKIYPLCESVCSNIPIYKSSGRHIDSLAIASALQDEWYHILLSGPGVFLLKGMYSDSLLDRSNRVFSSIIASEKSSSLSGGDHFAAAGNNDRIWNSFSKHCCADAASFMEYYSNPWLALACDAWLGPAYRITAQVNVVKPGGKAQVCHRDYHLGFQTAETAARYPRAVQVASQLLTLQGAVAHTDMPLDSGPTRLLPFSQLFEQGYMAFRLPEFNQYFLENYVSLPLEKGDGIFFNPALFHAAGENVTMDLERSANLLQISSAFGKPMEAIDTLALVERCWESLMGKYEKEGLSKGVEAFVAAITEGYPFPTNLDKRPPAPGGMAPESEQDVVLRGLKEAWTSGAVMQVLRRMRADST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAV3</accession>
    <name>A0A8H6CAV3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA repair protein RAD14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_003870</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149480.1">
        <property type="nucleotide sequence ID" value="XM_037294792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAV3"/>
    <dbReference type="GeneID" id="59332281"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000715">
        <property type="term" value="P:nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21077">
        <property type="entry name" value="DBD_Rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.530.10:FF:000003">
        <property type="entry name" value="Dna repair rad14 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.530.10">
        <property type="entry name" value="XPA C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000465">
        <property type="entry name" value="XPA/RAD14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022656">
        <property type="entry name" value="XPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037129">
        <property type="entry name" value="XPA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00598">
        <property type="entry name" value="rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05181">
        <property type="entry name" value="XPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="XPA C-terminal" evidence="12">
        <location>
            <begin position="233"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="332"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6220045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="387" mass="43791" checksum="E097C092ADFB8015" modified="2022-01-19" version="1">MTERPSTPPPQTTPSNPGVLPRAPLTPEQVKNIEINRLKAKALRTQRESEARNNSDAPTSLKRSSSNLTSSNLAAGQKRPYSSTTAGVTSTLRDARSTTPAAKPGGFRPLDDIQPARNFTKFVEYDFSKMTDTKGGFLTVEDDPHNKALHALKAGEKPAHMTMKEWERHQLLRSLREQKAGPFEPGLSVLSKGAREGSDKKCRECGSLEIDWKWEEVLHCAVCNACKEKFPEKYSLLTKTEAREDYLLTDPELKDEELLPHLERPNPHKSTWNNMMLYLRYQVEEYAFSDRKWGGGDKLDEEFEKREGEKKRRKEGKFRAKLQELKKKTRVEAYRRGRRGDGGGEFGDEVGRGGKHTHVWGRPVEGDEGVGTKTCVECGMEVEELEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBF4</accession>
    <name>A0A8H6CBF4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149780.1">
        <property type="nucleotide sequence ID" value="XM_037294399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBF4"/>
    <dbReference type="GeneID" id="59331888"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="175" mass="19154" checksum="7DD416C1206F8A21" modified="2022-01-19" version="1">MPTVHSLPNTSTHGTDQIYSPAAAQRLPTHASSLRTPTSPRASFVTDSRANINYTVQCTTTHSSRARHSPTSTSCLPRSANPTSPPALPTDAAYQFEFLQTSTLNIVAKTGKTARMTETRVFRLQNEEWSIWNVDGDGAVMGELAELALGHVIEPPIDFGPHQFQEAQLKAVQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBH1</accession>
    <name>A0A8H6CBH1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002874</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149877.1">
        <property type="nucleotide sequence ID" value="XM_037293799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBH1"/>
    <dbReference type="GeneID" id="59331286"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02908">
        <property type="entry name" value="Macro_OAADPr_deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF27">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="2">
        <location>
            <begin position="32"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="342" mass="36390" checksum="A34BBC708E665505" modified="2022-01-19" version="1">MAPPVQLSQIPTLSDLYKNGTLQRSTPDPTETPLPTPSATLNAKVSLIRTSITNLATTCIVNAANTSLLGGGGVDGAIHSAAGPSLLSECRLLNGCPTGSAKITSAHALPSSYIIHAVGPIYHRHRKAADKLRSCYRTSLELALEKGKEEGKNASIAFSCLSTGVYGYPSGEAAEVAGWEVRRFLEELEEDREKKGGLERVVFCIFEAKDERAYGEWLPKIFPPTPEDLSAKEEPAESESLPAKPAAGEAPAVSKDTSTGAPQAKKLKTSTEDLGKDDWEAVEKPNEATSDEAADMSEEGEKVEAVEFGGSDGEKVGKSIEEQKGESKADAVQSENMLAKDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBL7</accession>
    <name>A0A8H6CBL7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002976</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149978.1">
        <property type="nucleotide sequence ID" value="XM_037293900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBL7"/>
    <dbReference type="GeneID" id="59331388"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034946712" description="Peptidase A1 domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="2">
        <location>
            <begin position="109"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="508" mass="55385" checksum="B9B2801CCAC4EA2B" modified="2022-01-19" version="1">MHLLPLLTLTSGLLSYTLASAPALRSLDTAPVLHFTLTRRGGKFAPTEFARDYVNLTYLAEELEKTEARFNLTKREVKGNKLVRKAKNNEVGGRDPSALMGEVAADGIWYAKVKIGDPPQEIEMDLNMLVSDFYVVTTTSRKGSRYDDFFSQSAQKSLDRPYPTCYQHTDLFTLPAVNDPLPLSFPYCRPSKFSRNTLGPSGSSLGLAPSEHLSQTGSISLFKQLLEKEVIQHPIFSLMLINGQDGVLSVGGTAASTIEMVVSQTENALNQLDGTEKGDVGPVEKLAPLVKRGRHGKEVVTKREDWETGWVWTGVQGAEGWWQLLMRGVWVDGSKVLQNQAVVIDINSPFILAPPLAVKAFYASISGSRPLPPPFSNFYVFPCLNPPTLHFEFSGTPFPFMQGGRGAEWSGIPGGKFSLGRLEAGSGYCVGAVVETRMGIREERDEVVHDGKRGRSGPSRGGLGGNGMRDVWVIGEGFFRGVGGVFDVSLSSALAKSGYSEIFARLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBT3</accession>
    <name>A0A8H6CBT3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149766.1">
        <property type="nucleotide sequence ID" value="XM_037294385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBT3"/>
    <dbReference type="GeneID" id="59331874"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="150" mass="16334" checksum="E457AEAC556E2AC4" modified="2022-01-19" version="1">MQSFPNSVGSISPRSGNYVAPATWTAAEPAVAVISACLPSLRPLFVRIIWGQAYRPTPTSQPSNKRSYSASWRSGIPSKGTNGSFDRLQDTISGSSKTPWRNNVNVYGGQDTGSDEYELGNPQEALPARGIRVKTTVTVTERVVWQDNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBY9</accession>
    <name>A0A8H6CBY9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150102.1">
        <property type="nucleotide sequence ID" value="XM_037294024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBY9"/>
    <dbReference type="GeneID" id="59331512"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02219">
        <property type="entry name" value="cupin_YjlB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014500">
        <property type="entry name" value="UCP019307_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047121">
        <property type="entry name" value="YjiB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36448">
        <property type="entry name" value="BLR7373 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36448:SF3">
        <property type="entry name" value="CUPIN TYPE-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019307">
        <property type="entry name" value="UCP019307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-1" evidence="1">
        <location>
            <begin position="75"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="182" mass="19974" checksum="AD21EA88A6D7CAEE" modified="2022-01-19" version="1">MATSKAPLTSLRDLRVSSHQIPAFELIPNTSIQKKPLLIYHSAFHSSASASAIESHLSAVGVVSPEWRYTMYSQSHFHSTTHEVLCVASGKARLCFGGEENPKRVEPVLEKGDVMVVPAGLSHRLLEDINGGFNMVGSYPMGKSWDMCYGREGEEEKVKGIGSLGWFERDPIYGDEGPSLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCT7</accession>
    <name>A0A8H6CCT7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6594 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150504.1">
        <property type="nucleotide sequence ID" value="XM_037293675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCT7"/>
    <dbReference type="GeneID" id="59331162"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046529">
        <property type="entry name" value="DUF6594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34502:SF5">
        <property type="entry name" value="DUF6594 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34502">
        <property type="entry name" value="DUF6594 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20237">
        <property type="entry name" value="DUF6594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6594" evidence="2">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="326" mass="36549" checksum="8385F130DD4F5E49" modified="2022-01-19" version="1">MASLMGANPEVAILRRFGAMNAQNLLYLQAELVALEDDLRYYADQDSASDILDRNRYSECWDDLSRSDDKSSGSDERAGQQWRTMLRIREKLKEYNEALLHQTALAGLQKPNPRDLSFLQQWMESVSMGNVHLVGQDSDVWSKPDKSDLIALQARHADDPLTAWVTNTGVHWWHQVVGKYLRQPEATNWSANTVSYSQTGLSRLPSLVGVVLASLLPIASIVVLYTISSDLVRLIVIGLFTAGFSTSLKLVTNARLVDLFTATAAHVDSVNGRRMILKMHHTVKRDCRTFGMGERAGFAGPKRAKTVEVPLQRRFTVKVVESFLQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCW8</accession>
    <name>A0A8H6CCW8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002684</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150438.1">
        <property type="nucleotide sequence ID" value="XM_037293609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCW8"/>
    <dbReference type="GeneID" id="59331096"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47651:SF17">
        <property type="entry name" value="DEACETYLASE SIRTUIN-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47651">
        <property type="entry name" value="NAD-DEPENDENT HISTONE DEACETYLASE HST4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="6">
        <location>
            <begin position="84"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="385"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="615"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="462"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="484"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="575"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="616"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="645" mass="70513" checksum="5AA296AFC604A807" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCY8</accession>
    <name>A0A8H6CCY8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150698.1">
        <property type="nucleotide sequence ID" value="XM_037293045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCY8"/>
    <dbReference type="GeneID" id="59330532"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="446" mass="48683" checksum="333D272A98C7A8DD" modified="2022-01-19" version="1">MSFNHKKNSLAPSKKQQSGTVLKKSSLLAGSRGNGKKPHGQGSQPMDASTGQQNYQTSGQGHVDMEQSSFSHSASIVRPDHRSCDQDRIAQQDRVAQWAANSATNPDSATGHAFPGYDSASYRNDMPVTNVAHETPGAFHFPYSDMMRDPSQFSSMYQSYGVEETYHDMGATSAGLQFSGMDMAFEMPQSHAFDMTQLIQGEQRYTNGSPVNNLAYQIMTTGGFFPGPAEIHPPMVLGNNDGGYALPAEWDHQVMYQENAVGVSLPLEYSPASGLAPSMSSSYSQHSFLGQLPDTPVSMDLHEDWPIGEAGMNENFPHFSAGGAMQIQPAFNLPTDSERFAFQTNDDNLLPSDFPLSTIRPSTSFQRIPLPMDAWVGPEMMDPAFGFSSYPGMDGSRRSSDGEAGRNARENPLYKAVPQEDGLYHCPFAATDGCLKSPEKLKCNYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDB6</accession>
    <name>A0A8H6CDB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homoserine kinase</fullName>
            <ecNumber evidence="3">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_002179</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13986"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150759.1">
        <property type="nucleotide sequence ID" value="XM_037293106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDB6"/>
    <dbReference type="GeneID" id="59330593"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF1">
        <property type="entry name" value="HOMOSERINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00958">
        <property type="entry name" value="HOMSERKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="11" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="12" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="15">
        <location>
            <begin position="76"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6221324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="372" mass="40454" checksum="93BB81D76BFCB282" modified="2022-01-19" version="1">MKIIRVPATSANLGSGFDVLGLALRSAPSASQRFGNFLELQIKDLGQESLSSAPWNCEIICQGEGSELLSSLVHENLVTQVALYVLRCHGKEAFPSATRVTLITAIPLSRGLGSSAAAIVAGVMLGNEAGDLGLSKERMFDYCLMVERHPDNIAPALFGGFIGAFMDIARIEIPLSEVLPKLSGDTDTSLLPIHKPPLSIGCYHHYRLNSDIKAIIVVPTFHSNTAEARSQLPYSYTREDVIFNLQRCALLPVLLGEKPLHAAKISEAMRDRLHQPYRTNLVPGLGEVLLELRYDKYPGLLGTCLSGAGPSVMALATQNFETIAQAIIMILSEAQDVHYDWQVLELDPDGATCNDTNQDFCHVEESESRLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDC4</accession>
    <name>A0A8H6CDC4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150852.1">
        <property type="nucleotide sequence ID" value="XM_037293199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDC4"/>
    <dbReference type="GeneID" id="59330686"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF64">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="48"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="601" mass="67426" checksum="1AD131EE3A8E1A10" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDL4</accession>
    <name>A0A8H6CDL4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GET complex, subunit GET2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002181</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150761.1">
        <property type="nucleotide sequence ID" value="XM_037293108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDL4"/>
    <dbReference type="GeneID" id="59330595"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028143">
        <property type="entry name" value="Get2/sif1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263:SF1">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08690">
        <property type="entry name" value="GET2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="31637" checksum="04D79F10A0DC26BB" modified="2022-01-19" version="1">MDSPEPQAQGGDVETPTQQQARLRRERREAKIKAGGSARLEKITNLSGRQNLPEPPPGTLPSSSPSTHGDPEEVDLSTHPSANRTPNSNNGAPTEADIRALLRGGAPQPGADGQQQQGEEDPMMKMLQQFMGGMPGAGGPGAEGEGGLPPGLAAMMGAGGMGMPGTGGTQTKQEDTYGYLWKIVHAVFALVLGIYVTATSHAFNGDVSRGGVAGAGHEGGVNVFWLFATAELVLQSSRFFLEKGTGSQLGGWIGIVGNMLPEPWKGYVRLAARYSGIWSTVVEDGMVVVFVLGCVAWWKGAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEK4</accession>
    <name>A0A8H6CEK4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Aprataxin-like protein</fullName>
            <ecNumber evidence="4">3.6.1.71</ecNumber>
            <ecNumber evidence="3">3.6.1.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Hit family protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_001189</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 3'-end 2'-deoxyribonucleotide-3'-diphospho-5'-guanosine-DNA + H2O = a 3'-end 2'-deoxyribonucleotide 3'-phosphate-DNA + GMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52140"/>
            <dbReference type="Rhea" id="RHEA-COMP:13186"/>
            <dbReference type="Rhea" id="RHEA-COMP:13187"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:136419"/>
            <dbReference type="ChEBI" id="CHEBI:136420"/>
            <dbReference type="EC" id="3.6.1.72"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end adenosine-5'-diphospho-5'-2'-deoxyribonucleoside-DNA + H2O = a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52128"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136413"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5'-end adenosine-5'-diphospho-5'-ribonucleoside-2'-deoxyribonucleotide-DNA + H2O = a 5'-end 5'-phospho-ribonucleoside-2'-deoxyribonucleotide-DNA + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13182"/>
            <dbReference type="Rhea" id="RHEA-COMP:13183"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136414"/>
            <dbReference type="ChEBI" id="CHEBI:136415"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.71"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.71" evidence="4"/>
    <dbReference type="EC" id="3.6.1.72" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151538.1">
        <property type="nucleotide sequence ID" value="XM_037292119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEK4"/>
    <dbReference type="GeneID" id="59329605"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033699">
        <property type="term" value="F:DNA 5'-adenosine monophosphate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120108">
        <property type="term" value="F:DNA-3'-diphospho-5'-guanosine diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990165">
        <property type="term" value="F:single-strand break-containing DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000012">
        <property type="term" value="P:single strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000017">
        <property type="entry name" value="Aprataxin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032566">
        <property type="entry name" value="Znf-C2HE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF4">
        <property type="entry name" value="APRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16278">
        <property type="entry name" value="zf-C2HE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aprataxin C2HE/C2H2/C2HC zinc finger" evidence="20">
        <location>
            <begin position="227"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6222103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="288" mass="33235" checksum="EC038E588ACDB244" modified="2022-01-19" version="1">MTQPAQNQDATTGEEIASTKVPPKGTLPERNAFSDLMSPKKPPPSKKVPPLDTPHSDPARTTTFLGRDGLGAYIASPASFPTSRVIYHDEKWVVINDLFPKSSVHILLLPRDASKQLLHPFEAFRDLDFLKDVQAEVQKLRRLAAKELHRRYSKYSALDQKRQKAIDALDGDSDGESRHLPIGRDWSQSVISGIHVGPSMNHLHIHILSVDRHSECMKHKKHYNSFSTPFLIDVEDFPLDRQDRRRHPSKERYMERDLKCWRCGKVFGNKFSRLKEHLDEEFEEWKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CES9</accession>
    <name>A0A8H6CES9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001263</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151612.1">
        <property type="nucleotide sequence ID" value="XM_037292193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CES9"/>
    <dbReference type="GeneID" id="59329679"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000031">
        <property type="entry name" value="Ubiquitin ligase subunit CulD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000035">
        <property type="entry name" value="Ubiquitin ligase subunit CulD, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cullin family profile" evidence="8">
        <location>
            <begin position="513"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="534"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="625"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="549"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6222177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="895" mass="102140" checksum="F28D10464355E48E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFH1</accession>
    <name>A0A8H6CFH1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001499</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151848.1">
        <property type="nucleotide sequence ID" value="XM_037292429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFH1"/>
    <dbReference type="GeneID" id="59329915"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF7">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE-LIKE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="18"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="357" mass="37946" checksum="43F6F5B41406EA30" modified="2022-01-19" version="1">MAPQIPKTHKAAMFKEKGGPLIVEEIETKQPQEGEILVKVQACGVCHSEVVVQQAMFGTPFPRIPGHEIIGHVVAVGPHEKEWKVGDRVGGAWHGGHDGTCKQCQRGFNQACVNEQINGVTRDGGFAEYCTLRSEAAVHIPTDVDAASYAPLLCAGVTVFNSMRQMKITSGETVAIQGLGGLGHLALQYANKMGYKVIALSSTGAKEKFAKDLGAHVYVDGSKENHAEALTKMGGAAMIVSTAPIPAIMGDLVNGLAPGGKLVLLARKLHPRFHTGGPLTMIAIGEITVNTGPLIAKGASVHGWPSGQALDSEEAIDFAELHGVKCMIEKFPLAKANEAYDYMLAGHPRFRCVLVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFQ6</accession>
    <name>A0A8H6CFQ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001462</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151811.1">
        <property type="nucleotide sequence ID" value="XM_037292392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFQ6"/>
    <dbReference type="GeneID" id="59329878"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000011">
        <property type="entry name" value="50S ribosomal subunit protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_uL15_bac-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934:SF11">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="113"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="49"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="292" mass="32502" checksum="E950F4D8AB51FABF" modified="2022-01-19" version="1">MPPRPFNLLRVSQSKFRPATELSPFLVPFLYTQQRGAHILASLADNPGALHKRIRRGRGPSSGKGKTSGRGHKGQKQHGKVPRGFNGGQTKDEVVHGKRGDHNFSNFSVDMKPLNLYKVQDWVDQGRLDPSKPITIKELLQSRCITNVKDGVKLLAKGAEELKAPLNVVISRASAQAIAAIEAAGGSVTTRYYTRFAMRRIMQGKMDPINSLQSQIKEGEEMSRKSFYNRLPDPTSRKDIEYYRDPAHRGYLSHLVPEGHGPSLFFKTPGTGKMAMRKTSKSKAVAGDNRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFR7</accession>
    <name>A0A8H6CFR7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000706</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152005.1">
        <property type="nucleotide sequence ID" value="XM_037291643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFR7"/>
    <dbReference type="GeneID" id="59329124"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051164">
        <property type="entry name" value="NmrA-like_oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748">
        <property type="entry name" value="NITROGEN METABOLITE REPRESSION PROTEIN NMRA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748:SF26">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="2"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="278" mass="30978" checksum="B19484D4A1AE066C" modified="2022-01-19" version="1">MVCGDLDHPDSLTAAFFGAHAIYAITDFWQPSYNPSNKEKLREGQTTNELCFDQEVQQGKNISNAAANGRTPERYVCSSLSDVKKWGRGKYTWVYHFDGKAKVVKYIRDELPDLAKRMSVLQIGLYATHWQAAPLLAPPKQLDGTYVLSLTADPDKPMPMIDTRRDTGHFVRALDQTPPGKNVLAYGSMISWKDIMTLWAKILRLPGGCYKQVTIDTIDWIAPGGLGREVAEGWAFQGEFGYDGGDPSIVHGKDLTVDIPTTSIEDYIKKEDWSSVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG43</accession>
    <name>A0A8H6CG43_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_000806</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152104.1">
        <property type="nucleotide sequence ID" value="XM_037291742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG43"/>
    <dbReference type="GeneID" id="59329224"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051711">
        <property type="entry name" value="Stress_Response_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540:SF1">
        <property type="entry name" value="ACTIVATOR OF STRESS GENES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540">
        <property type="entry name" value="THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="621"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="691"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="887"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="706"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="724"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="778"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="904"/>
            <end position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="942" mass="105778" checksum="D374568D252744DF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGA6</accession>
    <name>A0A8H6CGA6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152181.1">
        <property type="nucleotide sequence ID" value="XM_037291819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGA6"/>
    <dbReference type="GeneID" id="59329304"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="133" mass="14026" checksum="CC5186A78117B565" modified="2022-01-19" version="1">MCFIRINPSIAPLNPPGAAPTLTTIYDEAAATIQTSSATTSPSNSLSKEPPTPMQNSKRRMRSNPFRVKAAKEGVVGTGKEKRRTQTIADSLDLGGDLQMDAENGGSRVVGEGDEILVRRKTVATEIGSGSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGD6</accession>
    <name>A0A8H6CGD6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_000761</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152060.1">
        <property type="nucleotide sequence ID" value="XM_037291698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGD6"/>
    <dbReference type="GeneID" id="59329179"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1690.20:FF:000003">
        <property type="entry name" value="Mitochondrial inner membrane AAA protease Yta12, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="444"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="47"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="195"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="862"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="871"/>
            <end position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6222714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="910" mass="100307" checksum="948E01B40A4283BA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHD0</accession>
    <name>A0A8H6CHD0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1770-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152807.1">
        <property type="nucleotide sequence ID" value="XM_037291372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHD0"/>
    <dbReference type="GeneID" id="59328852"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140580">
        <property type="term" value="F:mitochondrion autophagosome adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013898">
        <property type="entry name" value="Atg43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38699">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38699:SF1">
        <property type="entry name" value="MITOPHAGY RECEPTOR ATG43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08589">
        <property type="entry name" value="ATG43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17229" checksum="AC8929409D7B90C7" modified="2022-01-19" version="1">MSNPAVDIASTIQSASIKHNPDPAHDLNPSTAASTKRPVSPTTSIASSENSIPSSAVKPVPRRTNLPPLPDLRFEQSYLASLHGVDTWYGIAWITGRDQVLLPLVQGTLWTLALSGWRHWNRGASFSGRNLGGKVRRWWWGVNNWSIPSIPEDEVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHR6</accession>
    <name>A0A8H6CHR6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Rheb small monomeric GTPase RhbA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000309</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Lipid-anchor</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the small GTPase superfamily. Rheb family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152683.1">
        <property type="nucleotide sequence ID" value="XM_037291248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHR6"/>
    <dbReference type="GeneID" id="59328728"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04137">
        <property type="entry name" value="RheB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000273">
        <property type="entry name" value="GTP-binding protein Rheb homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6223466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="20806" checksum="5BA4F58D0C92215C" modified="2022-01-19" version="1">MPTSPKQRKMAVVGSRSVGKSSLTVQFVDGHFVDSYYPTIENTFSKVIKYRSQDFATEIVDTAGQDEYSILNSKHFIGIHGYMLVYSVASRQSFEMIRILRDKILNHLGADWVPLVIVGNKSDLKADQRHVQAEEGKKLAEEFSCSFTEASARLNTNVGKAFEQMVAEMEKSQNPGEPTGGSKCSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHS5</accession>
    <name>A0A8H6CHS5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000597</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152971.1">
        <property type="nucleotide sequence ID" value="XM_037291536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHS5"/>
    <dbReference type="GeneID" id="59329016"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070290">
        <property type="term" value="F:N-acylphosphatidylethanolamine-specific phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024884">
        <property type="entry name" value="NAPE-PLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032:SF4">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038896">
        <property type="entry name" value="NAPE-PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="3">
        <location>
            <begin position="108"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine</name>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine</name>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR038896-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="388" mass="42773" checksum="F53C6EF03FD6B6AE" modified="2022-01-19" version="1">MAMASATSITITRVPRKAESSQPSHHIGNPPTSFTNPWPSFHKHSPLDLLITSQRNRDFKPVPSRDKLVAIQKPDWGAGKEGLKATWIGHASFLVETSASEGAGRGVRVLFDPVFSERTSPVTWAGPKRYTPTPCTLDELPDVDLVVISHDHYDHMDSDTLLKIYASRKGNVHFLVALGNASRLRGFGIGGAEVTELDWWEGVQVDVPGMGSVTMTCTPSQHFSGRGIWDQASTLWCSWVLDAGAFSNSSATTQASTSRKVFFAGDTGYRYVPKEAKELDRPSCPAFKEIGEVFGPFDLSMLPIGLYSPRHLMSPIHCNPEDSLCLHKDLKSRKSIGMHYGTVRGGISQYFEEVTEPPRRFREACEKDGKVWGEELGLLNVGETLVMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHV2</accession>
    <name>A0A8H6CHV2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Guanine nucleotide-exchange factor SEC12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_000458</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the WD repeat SEC12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152832.1">
        <property type="nucleotide sequence ID" value="XM_037291397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHV2"/>
    <dbReference type="GeneID" id="59328877"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <keyword evidence="2 10" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6223615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="670" mass="72168" checksum="45717DBCC2E1B448" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI70</accession>
    <name>A0A8H6CI70_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone chaperone RTT106/FACT complex subunit SPT16-like middle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010526</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Histones H3 and H4 chaperone involved in the nucleosome formation and heterochromatin silencing. Required for the deposition of H3K56ac-carrying H3-H4 complex onto newly-replicated DNA. Plays a role in the transcriptional regulation of the cell-cycle dependent histone genes by creating a repressive structure at the core histone gene promoter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with histones H3 and H4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RTT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153012.1">
        <property type="nucleotide sequence ID" value="XM_037301378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI70"/>
    <dbReference type="GeneID" id="59338916"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF3">
        <property type="entry name" value="HISTONE CHAPERONE RTT106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="5">
        <location>
            <begin position="227"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="322"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="414" mass="46178" checksum="80CED674B8D99410" modified="2022-01-19" version="1">MPSKHIFDEAFAQRPDLRQRVLGAIAKAPEQESLFGDIAHYIKHVQHASVQEQFKGQDEQPFKKRKLEHRPIDTGGWDKKNVCEVTDVSFSIPQRKKLHLSINKSASSGLHAINASGTSEFGVTWEWVRDIVCLPVPEKAQAQYNFCIFSTTADGLAPDQIVWTVPDSVPKDGTFVGLTTSPGETCRTLLITVLNEALKPYKKRVIEPDKQEFASQVVQAHRRGEPAYHIKAFRGSKDGFLFLLANGIFWGFKKPLEFFSFDSVDSVSYTSVLQRTFNLNIAARAATDAEPQEFEFSMIDQADFAGIDDYVKRHELQDASMAEQRRAKKLNVNGLKGNETKQEGVDQEEGELEKATREAEDLEDEEEDDENFDPGSEGESEGSGSGSDEDEDEHEGRAGSNLVDEELASEAESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI77</accession>
    <name>A0A8H6CI77_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152977.1">
        <property type="nucleotide sequence ID" value="XM_037301343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI77"/>
    <dbReference type="GeneID" id="59338881"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="430"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="677" mass="78268" checksum="04F4CF7613B1A7C3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIH7</accession>
    <name>A0A8H6CIH7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010795</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153280.1">
        <property type="nucleotide sequence ID" value="XM_037301646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIH7"/>
    <dbReference type="GeneID" id="59339185"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="84" mass="9320" checksum="B6E52791959CBC51" modified="2022-01-19" version="1">MFPLSPADAFASHNKVDHLIGIDQVLQIDDAVGESLKATDITKWQPWKARKTAVEAVRDSISNQRATYGIINSKAFIDDQLCAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ08</accession>
    <name>A0A8H6CJ08_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_010685</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RSP5/NEDD4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153171.1">
        <property type="nucleotide sequence ID" value="XM_037301537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ08"/>
    <dbReference type="GeneID" id="59339075"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd08382">
        <property type="entry name" value="C2_Smurf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000011">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000017">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000053">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000074">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 9 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="393"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="15">
        <location>
            <begin position="482"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="139"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="284"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="284"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10 11">
        <location>
            <position position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6224111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="815" mass="91766" checksum="840D40B5A4F1EA04" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ49</accession>
    <name>A0A8H6CJ49_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1168-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_011036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153519.1">
        <property type="nucleotide sequence ID" value="XM_037301885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ49"/>
    <dbReference type="GeneID" id="59339426"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009548">
        <property type="entry name" value="Prkrip1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13507">
        <property type="entry name" value="PRKR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13507:SF0">
        <property type="entry name" value="PRKR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06658">
        <property type="entry name" value="DUF1168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22008" checksum="96E65B1037302BD2" modified="2022-01-19" version="1">MSEPIPESIPTSSDPRSKRPLKRRALTPVTQQASQVDALFAKPDRDIPIPSSTALAKKSALSAPPEIVANVQGSSAGAGSGEFHVYKASRRREYERVKMMDEEVAREKADEEFERKKEETRKKDEEKTGKNRKRREKKKKNKGKDGNEEADHDNQGGNMNGMSKLDRVTLPGPKQDVESGDALPATTAEDVGVIIHDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ61</accession>
    <name>A0A8H6CJ61_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peroxisomal ATPase PEX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peroxin-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010745</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153231.1">
        <property type="nucleotide sequence ID" value="XM_037301597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ61"/>
    <dbReference type="GeneID" id="59339135"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19530">
        <property type="entry name" value="RecA-like_NVL_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000081">
        <property type="entry name" value="AAA family ATPase/60S ribosome export protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000149">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="205"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="508"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="735" mass="80456" checksum="59CC0158E2A9183C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK46</accession>
    <name>A0A8H6CK46_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA damage-inducible protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_010024</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable aspartic protease. May be involved in the regulation of exocytosis. Acts as a linker between the 19S proteasome and polyubiquitinated proteins via UBA domain interactions with ubiquitin for their subsequent degradation. Required for S-phase checkpoint control.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds ubiquitin and polyubiquitinated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DDI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153697.1">
        <property type="nucleotide sequence ID" value="XM_037300880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK46"/>
    <dbReference type="GeneID" id="59338416"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05479">
        <property type="entry name" value="RP_DDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14309">
        <property type="entry name" value="UBA_scDdi1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01796">
        <property type="entry name" value="Ubl_Ddi1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057273">
        <property type="entry name" value="Ddi1/2_HDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033882">
        <property type="entry name" value="DDI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019103">
        <property type="entry name" value="Peptidase_aspartic_DDI1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12917">
        <property type="entry name" value="ASPARTYL PROTEASE DDI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12917:SF1">
        <property type="entry name" value="AT13091P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09668">
        <property type="entry name" value="Asp_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24669">
        <property type="entry name" value="Ddi2_HDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="14">
        <location>
            <begin position="11"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="13">
        <location>
            <begin position="425"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="365"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="401"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6224830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="463" mass="50255" checksum="13EB25C2D1C99615" modified="2022-01-19" version="1">MFESLPNASTITISINTIDAPQVTLEQDFLTADVGPHMTIADLKGYIESETNISAAAQRIYYGNRELVDDSRTLQQCEMTNDSMLGVQVRNPQAPSVGSSRPVRTQRRGPVQAGSRQSAQADPEMIRLQAMGNPNILATIRASDPDLANAVQDPARFQQIFEEKLRQKVEMDVEFQRRNDLLSADPFNVDAQREIEEMIRQEGVIENLENAMEHNPEGSTLYSRNDFLVFARVHMLYIPVEVNQHKVKAFVDSGAQTTIMSPSCAEKCGIYRLLDKRYAGIARGVGTAKILGRVHSAIIKIGTSFLPCSFTVMEGKDVDLLLGLDMLKKHQACIDLQKGKLIIQGDEVEFLGEADIPRYEDVYKNEPTIEGPAGTRTGAISGTVTQGASSAGTSQVTAIGPSASASSSSGPGSTQQSQSTTGPASFPKEDIEQLTNMGFTKQEAIHALEMAGGDVEMAAGLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKR6</accession>
    <name>A0A8H6CKR6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010374</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154044.1">
        <property type="nucleotide sequence ID" value="XM_037301227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKR6"/>
    <dbReference type="GeneID" id="59338765"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="112" mass="11311" checksum="FA5F777078B1A4B6" modified="2022-01-19" version="1">MAMKAPRHPPTDATALIVAPVVLPLPVPSDEDAAVEEVVVDVVGKGGARVVAAALLATTAGFEDVEAVKLEELIVEASVGVADAAQAHTALAKAPNGYGTAAFEDARERAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLE9</accession>
    <name>A0A8H6CLE9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD-dependent 15-hydroxyprostaglandin dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009688</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154397.1">
        <property type="nucleotide sequence ID" value="XM_037300549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLE9"/>
    <dbReference type="GeneID" id="59338083"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000643">
        <property type="entry name" value="Short chain dehydrogenase/reductase family oxidoreductase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229:SF4">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="35939" checksum="4A2DB54957722393" modified="2022-01-19" version="1">MALPLAGLAGKTAIITGAGSGINLSFARALLTKRCNVVFADLALRPEAQELVSNHANRSQSPAKAVFQKTDVRDWQQLERMFHVASKEFGGADIVCPGAGVYEPPFSNFWHPPGKPPSEDDPTSNRYAILDINLTHPIRTTQLAISHFLSSSSSSSSSNKEKSSPKSIVHISSIAGQVTPLPGPIYNATKHGINGFVRSLAPLDSRLGIRVTGVAPGVIKTPLWTEAPEKLKLIADDDAWVTPEFVADVMVSLVENQTVRVSAGLGATGSRLSSGDAREGTKEVAVEGGMILEVAKGRVRVVEQFNDVGPSGEGNTVGNMRVAEEEILERLAGGGWGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLI2</accession>
    <name>A0A8H6CLI2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009857</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154564.1">
        <property type="nucleotide sequence ID" value="XM_037300716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLI2"/>
    <dbReference type="GeneID" id="59338252"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057082">
        <property type="entry name" value="PH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057081">
        <property type="entry name" value="PH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23076">
        <property type="entry name" value="PH_FT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23074">
        <property type="entry name" value="PH_FT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="292"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="417"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="537" mass="60850" checksum="302E41214BE048F9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMK2</accession>
    <name>A0A8H6CMK2_9LECA</name>
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        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_009143</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000006">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037300004.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
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    </dbReference>
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        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
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    </dbReference>
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        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
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    </dbReference>
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        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
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        <property type="entry name" value="His_kinase_dom"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="204"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="299"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
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            <end position="443"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="575"/>
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        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="667"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="12">
        <location>
            <begin position="741"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="1119"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1253"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="1168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6226277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1327" mass="144507" checksum="99C2DE600022B06E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMM7</accession>
    <name>A0A8H6CMM7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154750.1">
        <property type="nucleotide sequence ID" value="XM_037299924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMM7"/>
    <dbReference type="GeneID" id="59337458"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="38176" checksum="CCF4FFE2D9F6FC88" modified="2022-01-19" version="1">MPLDHYYNSGVEVIYPSQMMNPHHFSEHEWNPTPLVPLHPHEFDNTPYSSGPHIPPPISITHPSHIIPFSRPKSSPSTFGPNVEDLLFTENPFRIKNEDYAPPMFSMSPQPVPSTKTPSPYYSGDYPVSNPGASPGRHGPVPIAPDPVGLRQMQTLKRMREDEEYSEYGQKKRKRAPSQAGPVELNEEEQLLLKLKEEENLPWKDIAVRFQTDLGKSYQVPALQMRFKRLRERMRTWTETDVQALEQAYDYWEKFQFDIIAAKMLDFGASERWPAKYCARKWEEIHPGRGPFSTPSNFLQDPWDATSPVESLSHSHSPRQTQFTHSPRQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMU5</accession>
    <name>A0A8H6CMU5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Photolyase/cryptochrome alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009194</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="6">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154881.1">
        <property type="nucleotide sequence ID" value="XM_037300055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMU5"/>
    <dbReference type="GeneID" id="59337589"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.579.10:FF:000003">
        <property type="entry name" value="Deoxyribodipyrimidine photo-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF18">
        <property type="entry name" value="SI:CH1073-390K14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="9">
        <location>
            <begin position="91"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="343"/>
            <end position="347"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="386"/>
            <end position="393"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="484"/>
            <end position="486"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="7">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="7">
        <location>
            <position position="471"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="7">
        <location>
            <position position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6226328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="586" mass="67056" checksum="AD75A115D3A50877" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMZ1</accession>
    <name>A0A8H6CMZ1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154930.1">
        <property type="nucleotide sequence ID" value="XM_037300104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMZ1"/>
    <dbReference type="GeneID" id="59337638"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034416075" evidence="2">
        <location>
            <begin position="21"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="118" mass="12607" checksum="5657B905E00DE8A5" modified="2022-01-19" version="1">MANAVSVILCSWALLSCVDALVNTNFTGTHKTSAVIFDNEFSLIVCIVSVEDLERVALEDDGKDGFEEGPDEDERADEKGEGDEDEIMSGGNGSVELIADNEYENNEKSVGDAAVDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN49</accession>
    <name>A0A8H6CN49_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155066.1">
        <property type="nucleotide sequence ID" value="XM_037300240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN49"/>
    <dbReference type="GeneID" id="59337774"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="327" mass="35788" checksum="75F51C2B82BE60C5" modified="2022-01-19" version="1">MATSASPSPMAPPASSSPMAPPASSSPMTPSPSSSSPMVPLAPSYSPMAPPASPSSPMAPPALPRELYPFNVARGDFLYKTVNQAEWTRLPSPIDDPQHNCYGIRFDGTVTLTQKHRERGSMCRVKLILKPPIIKTEDEQTTVEAVCIRIGNPSFSAYPECHEKVKAVEHAKTLPLATLDKTITLVKLRNLKLRNLSVSREEAKKADNYELGERIWALIDYGYRGAAPPGQTKKINWTVLPRPSSSGSAETPTDKRTLKERLQAHQVATAEGESRLRQGLEDDRQAMVRKVTEAINGEWDKKVAMAVAEYRAQTASTMEKIVAEGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNG0</accession>
    <name>A0A8H6CNG0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_009330</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155017.1">
        <property type="nucleotide sequence ID" value="XM_037300191.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337725"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
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    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="18">
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        <location>
            <begin position="161"/>
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        <location>
            <begin position="1549"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SMART" id="SM00663"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6226464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1751" mass="192808" checksum="8619FD39C54E85A1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPK1</accession>
    <name>A0A8H6CPK1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008690</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6227248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037299555.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="Znf_RING_CS"/>
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        <property type="entry name" value="BIFUNCTIONAL APOPTOSIS REGULATOR"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR15898:SF13">
        <property type="entry name" value="BIFUNCTIONAL APOPTOSIS REGULATOR"/>
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        <property type="entry name" value="zf-C3HC4_4"/>
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            <end position="88"/>
        </location>
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            <begin position="1"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="680" mass="75192" checksum="89446CFD74E4DA68" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS20</accession>
    <name>A0A8H6CS20_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_006951</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156482.1">
        <property type="nucleotide sequence ID" value="XM_037297847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS20"/>
    <dbReference type="GeneID" id="59335351"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="11"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6228840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="32467" checksum="1EED683C2DEA67ED" modified="2022-01-19" version="1">MPVRGNHLEKWRVLRTIGEGAQGRCKLVERKSDGELAVRKQVDWYTKINSSPLEAIILNDVLPPSRRVVKLITFWFTPSPRNDDYLIELFEYCRGGDLKHAVTQYGRLSEDFIWHCFIQIAEALDVVHNAGSQLVVHRDVKPDNIFLEQKYRHAAPWPNLKLGDFGAATLKPHTEAMLMRCWQGPELPHHSTAGDIWGLGAIIHWMGYRRPPITPRPARFSQKEWESQPAARRPTLLPNSYSLELNDCMMECLRWDPRDRISSHELVKRLKRDRHGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F705</accession>
    <name>A0A8H6F705_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147210.1">
        <property type="nucleotide sequence ID" value="XM_037297500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F705"/>
    <dbReference type="GeneID" id="59335003"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="265" mass="29927" checksum="070B39A7480BABD3" modified="2022-01-19" version="1">MSYPTPATRNVRALRIPADGSPPHLITLETMMTEPVPPPPSNSYEGLLSHLGKTEKARHVPNVKAFWTLRGWEDRTCSWVYYGNGSMKDVEGMYYIFLAVDHDRLPPNKHLSTDLFDCCGDVFLLKMVNWPEKRAADYDDPIDCLRHLRHEHLFPEYADVDDRVLGSDLLEATVRELLRGAVLAMRNRGEMVDEDKDDSPDGNLPRRDHKGERVHGVALVIAERPQCGLASWILVDLAITSVASSEGAGKQCFLEHSEQIKIIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7A9</accession>
    <name>A0A8H6F7A9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147091.1">
        <property type="nucleotide sequence ID" value="XM_037297655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7A9"/>
    <dbReference type="GeneID" id="59335159"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="474" mass="53908" checksum="31F6176DABAFF916" modified="2022-01-19" version="1">MSSSSRSRKIFDEILRGDSSLPSYADSSRDASRPTAPTPRSIQDDHRAPTFPQNPAPPPPLAPPPANTRPRRTPAVGFSAFPRHPVMAKHFASVSNTNTGGTFSEERKSSPMPYKKTAIPPETLEEHSIYPVGDRIRKVPQSEESLDSQMGPERGYSRNPELNLQYQVRNEIRRLWEEKHSKTAEDTDRLDRDTGHQSGVVKSDGDPTIREEIPNPRRVLHESLEWHDDSDAEDLDKDLTVFADTPRDEGIVEERGEWLLPPWDVPDPIHAPSLTALSSMTEWDPEADIDFQTEARAGWSDCRFKVPQTPADIESLQQALEITRMDFWIRNPNERYPEDLSQYKGESYGSQHRRLQHAFCTIWQGLETEAPPALYRLPEWMFGFEYCYWKPSSWGYNKRSNAYNEGLAAMAAEKNEKSLENLRYQDWKAKLKEVSGAPTLADFAPSRRSVAPEEIERGGNEGLYSVSPTPDFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7F2</accession>
    <name>A0A8H6F7F2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146938.1">
        <property type="nucleotide sequence ID" value="XM_037297737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7F2"/>
    <dbReference type="GeneID" id="59335241"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034386276" evidence="1">
        <location>
            <begin position="21"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="273" mass="31303" checksum="BAFEB6377A944FE4" modified="2022-01-19" version="1">MPLLQTFLTALTLLPSISLAVAPAQVPPNGKGLGFISNLEDLYPEPTGDWLTSTLCYCHSPIHAKEKWQYEKAHIFQHEYYNYHSNATFIVDHMCLSRAHTEGDQCNRPNVGGDNNDWLMQKRYVCKDFPRTEEEKVRQANSKRSTRRGKRAEPIAQHMVSCVQDCDPGAYAPDVPDPSPHPKFDRVCFGVDTDFYGMDEMELKFNRQHRKMESPGQQGRVQTEFEEVQGYCEDMCQSMFKMPVDMKINDKRAGGGSRQFLYTSLDDMCDHCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F865</accession>
    <name>A0A8H6F865_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148200.1">
        <property type="nucleotide sequence ID" value="XM_037296218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F865"/>
    <dbReference type="GeneID" id="59333714"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="152" mass="17118" checksum="CE8EE9500266DB0B" modified="2022-01-19" version="1">MDIESPQAIVCDGQNRYLLESVGQYSDLLLQQDGQFGSTFQFKDSPIVSFIQTKSSPTAVKVNDSWKMAGPKGVLVDQFSATRPRLWESDDEGFHRTHSDLVEFAINDVDYERVLNRLRGITTKATGILTSRYLSQGRQRFLSTVLCVPPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F899</accession>
    <name>A0A8H6F899_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Gamma-glutamyltranspeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147853.1">
        <property type="nucleotide sequence ID" value="XM_037296672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F899"/>
    <dbReference type="GeneID" id="59334171"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052896">
        <property type="entry name" value="GGT-like_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881:SF1">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="609" mass="65246" checksum="71D2270C6D7D09E0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8H3</accession>
    <name>A0A8H6F8H3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148530.1">
        <property type="nucleotide sequence ID" value="XM_037295832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8H3"/>
    <dbReference type="GeneID" id="59333326"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034921553" evidence="1">
        <location>
            <begin position="20"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="489" mass="52156" checksum="9E6BDCC6B6D5F225" modified="2022-01-19" version="1">MLSKILIIAFAQSAYFAAAAPAANLPTNFDPEQFSIQPDNGGGPQDDCGNGPITLDVNTWNSHNMDALVQQFWDSGISNSNFDFHQAFASNYTDDLSCPDSFNNCVGDPSACNALSGSSYAKEQGWLGIKSILNVQAFFLQIEKVVSNAADGITGALFDFQTKFNPPDPPKSLSWVRTALSVTASVIGVGLSSISAIADSNLSAVAAAAYAAAASNSVTLVNNEAITDGTINASDITQVSDQWNNYKTALLSQIDNAHSATFSNGQYNGHAGARIIDYMSGGDFAGSSLVSAYESGEGNVQTLIESLMASKLLEATWIAQGAFIIFIPNVTNVCSKWKTNAGDNGERRLCAPEGMYLLNRYDATSGVGIATGMDPATVASWAGYNIDIHDLIQSSASIYQNHGLGASANMYDFVDFIKSYQSDPGAVFQQQGMFTVPVCQLQSYDWKQSDNMGLTTPPCDCLTATDKWGNKFMDATSTDIQNWINSKCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F917</accession>
    <name>A0A8H6F917_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149100.1">
        <property type="nucleotide sequence ID" value="XM_037295053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F917"/>
    <dbReference type="GeneID" id="59332543"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017771">
        <property type="entry name" value="Cyanamide_hydratase_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03401">
        <property type="entry name" value="cyanamide_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35569">
        <property type="entry name" value="CYANAMIDE HYDRATASE DDI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35569:SF1">
        <property type="entry name" value="CYANAMIDE HYDRATASE DDI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="63"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="242" mass="26669" checksum="D9DE334D7A00BBBC" modified="2022-01-19" version="1">MSAPSTLETHGWTAQPRDVSAFLGDKQDLKAPEPQTVASIALPSTPLAKAVLDYAKKELREETFNHSMRVYYYGKAIQTHLTPTHPLPVSDETYLLTCLLHDIGTTDKHLPATLMSFEFYGGYLALDLLKKDLRAPVEQAESVAEAIIRHQDLGVSGKISSLGLLIQLATIFDNMGGNAELVHKGTIEDVVRHYPRRKWSGCFAATIRRENGLKPWAHTTALGEEAFPAGVENNALMAPYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F952</accession>
    <name>A0A8H6F952_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THUMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005024</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148634.1">
        <property type="nucleotide sequence ID" value="XM_037295936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F952"/>
    <dbReference type="GeneID" id="59333430"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2300.10:FF:000001">
        <property type="entry name" value="THUMP domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2300.10">
        <property type="entry name" value="THUMP superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452">
        <property type="entry name" value="THUMP DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452:SF10">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="3">
        <location>
            <begin position="147"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="302"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="334" mass="37132" checksum="F21C0F751DF4A620" modified="2022-01-19" version="1">MEESKKRKSETSNGDNAQRKSKPWRVPRKGQPNYVNPTIEPGDSGIWATCELGKEGKSTAELRDLFEEYAKKLYGSLEEQADGEDSNEDVEADIEAEIKKEVQGMKSRVTKKETLFQSVKMNIQCVLFFKTRPPVEPVSFVHTICKDAAEGATQRARFVKRLSPMAMMGKATEDGLAKVADAVLGPVFHVEEDGLKFAIRPTRRNHNIMKRDDIIKQIAKAVGGDHKVDLKGYNRLILVDVYRNILGMSVVGSDYDELKQYNLAEIYDPTPKLKAKMSIGAAPEVKDKDDKPPSDVAPNGHGPKDDVIPMEKSEDDKTSLDSQKAADDAHLTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9G2</accession>
    <name>A0A8H6F9G2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003338</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149642.1">
        <property type="nucleotide sequence ID" value="XM_037294261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9G2"/>
    <dbReference type="GeneID" id="59331749"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035516">
        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051747">
        <property type="term" value="F:cytosine C-5 DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032852">
        <property type="entry name" value="ALKBH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31573">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31573:SF1">
        <property type="entry name" value="DNA OXIDATIVE DEMETHYLASE ALKBH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="184"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR632852-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="334" mass="37936" checksum="5BEC2F75D60EEC1B" modified="2022-01-19" version="1">MSKKRSLDSFFTPTSQKKVCLTVEAAPSEVNPVYTSSTEVSNHKTYPFPIPRLPPNIIERLNEVPASQGNEINDQPDLDLLYFQPYIPPATERELFEFLRNALFFYRVQYTIKRYGNETRINTPRFTTVYGVDTSSTFTPDGALIDSQTSKPVLPDRYKTCTPRPIPHCLDYLRQLTEIFTGATYNFCLVNYYANGADSISYHSDDERFLGPNPSIASFSLGATRDFCMKHKPIPPKDGEPPPEQNSKPPLKLPLASGDMVLMRGKTQANWLHSIPKRKGKGSEGGRINITFRKGVEKYSTQNYYQYNVGDGGVWKWDARKGEMKEWVKASEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9L4</accession>
    <name>A0A8H6F9L4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003534</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149144.1">
        <property type="nucleotide sequence ID" value="XM_037294456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9L4"/>
    <dbReference type="GeneID" id="59331945"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="234" mass="25609" checksum="DC1A9AEACF9A54CC" modified="2022-01-19" version="1">MNRYSCLQAFNTIPTIRQPLTFGDRSNGTFNVQLPRRFSGPDATCVIDIFHKDGVISDIASYQQISRAAESLYGVCVVRGGTRTQGGWIRDIGRQKNLVIVLSLYEPSVACFGQQFAMRDPAQISQKLLSIIPVNTERQIFGPEGESGVQVEVPVTYNWPPGPSRWAIGASLKVDVEHRNRVDARWFDVWAGAVAVTAICTLRGYAGISGLPSGLRVTLKAAPGPRVQNETDTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9N9</accession>
    <name>A0A8H6F9N9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003609</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149219.1">
        <property type="nucleotide sequence ID" value="XM_037294531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9N9"/>
    <dbReference type="GeneID" id="59332020"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.100">
        <property type="entry name" value="YAP1 redox domain. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF6">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="42978" checksum="B390F26890FE721F" modified="2022-01-19" version="1">MSQASPSEEASASNSASPPPGKPRREGNDSLWKRARRTLGTNSLTFLQPTTRPAGTAAPATGSGPAAPSKRREQVRHAQRTHRQRTQNYIKTLESEVVRLREAETSLMQERDKLQSQIVNLKNTIILSNIPLPEGTEDIGTSSPPLRPFADLDMPATVSYSNDALNHPRLHVNFPQRQEPTSQGLDYPTQTYPLSSPYQEHQQYTNVPQSAPDLPNDFSLNTINLSSSSSKHGPPANANATIVVDTIETAIDFVLALEHPCMPHIPYQDPPSADPANHMMMASTPLMARSPEPPQFNSTWTASGAIIKELLNLSSSINLEGEITPVEAWHRLHDHPDFWKLGRDQIENLKRELSTAVTCCGFGAVLDENVFWNAVNRSLASSAVASYAKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA99</accession>
    <name>A0A8H6FA99_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150151.1">
        <property type="nucleotide sequence ID" value="XM_037294073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA99"/>
    <dbReference type="GeneID" id="59331561"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="227" mass="25389" checksum="69D3876AA91D7CD1" modified="2022-01-19" version="1">MTYTLHPVTSDDASDIAAIFQAAFAEGHIMRYFHPRVPASVIWENDVRFIRGLIAQGDIYGERVTKAVDEDTGETVAFSAWQYPYTLTEEQRKEKEAQDRSAIDEPPEGSNNQFITDFCTQLFDGRKKWIVPEKTFFLHLLAVHPSHHRRGLGSMLIRPGLEAADEAGAQTYVEASPAGLPLYLRHGWEQVDGMAVDTRPYGGRGIEHKPFLMREPGAGSKLGKTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB18</accession>
    <name>A0A8H6FB18_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA replication complex GINS protein PSF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_002382</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS3/PSF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150961.1">
        <property type="nucleotide sequence ID" value="XM_037293308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB18"/>
    <dbReference type="GeneID" id="59330795"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11713">
        <property type="entry name" value="GINS_A_psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21693">
        <property type="entry name" value="GINS_B_Psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010492">
        <property type="entry name" value="GINS_Psf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055221">
        <property type="entry name" value="PSF3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22466">
        <property type="entry name" value="PSF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF3 N-terminal" evidence="8">
        <location>
            <begin position="5"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="76"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="181" mass="19972" checksum="5E7AF3F64C17C3C2" modified="2022-01-19" version="1">MGSYYDIDAILTDAQKVPCTFDLDIPGLGYLDENAGGDIKAGTRVSLPLWLSTLLAVQRLGPSSQQLCTLDLPASISPRVLAALKAGPKTVDLRQLASHYYELAARVLELFEEEEIVDVLGETFKERAAEIADHAHNPRGALGEAQDFLRGLDEMERQLFRSAHGSSNAMREWMGETKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB63</accession>
    <name>A0A8H6FB63_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151096.1">
        <property type="nucleotide sequence ID" value="XM_037292557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB63"/>
    <dbReference type="GeneID" id="59330043"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="391" mass="44223" checksum="7B1ECD37A92EB42B" modified="2022-01-19" version="1">MAKRPRFFINFDVSRSKAKRPRGQDSSLMLDLPGNIPSRSAEMIHRSFTTMEPRTGLEARRHVSWHAPPLQKSQDVSVSATMKNPEGWCGRSGQIQRTNFPRTQYPSPPMSEDIRCDERNITDSMSHSDRPHRTNSFPKTPYPAVSSTTTAQASLYAERDIADTTTSPPSPTTAPANGPPHTNPPFPHTHSPLPAPSSYPIPQTFFAYPPIAPFDQSLKRHPNYYHSTHNLAQEIEPENGSRAVRLRHPDHLLERRRQKLGFAPTALVYEYGQEGRRDMPMRPAGTKFHDDGGDGDGGSILAGPWPRYWFSGRMNVLDLKFLGQAHDPVAGRKKVWYDSVKKDYVWTTLEEAKDMSGREERMREKAMARRRCDSEADTEMVEGEVGSWEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBE4</accession>
    <name>A0A8H6FBE4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001256</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151605.1">
        <property type="nucleotide sequence ID" value="XM_037292186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBE4"/>
    <dbReference type="GeneID" id="59329672"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027842">
        <property type="entry name" value="HAM1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045967">
        <property type="entry name" value="HAM1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31138">
        <property type="entry name" value="CHROMOSOME 19, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31138:SF1">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14613">
        <property type="entry name" value="HAM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19343">
        <property type="entry name" value="HAM1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAM1-like N-terminal" evidence="3">
        <location>
            <begin position="3"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="HAM1-like C-terminal" evidence="2">
        <location>
            <begin position="644"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="831"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="904"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="872"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="917"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="933" mass="102059" checksum="D007E69E93B05A6C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBJ9</accession>
    <name>A0A8H6FBJ9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151817.1">
        <property type="nucleotide sequence ID" value="XM_037292398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBJ9"/>
    <dbReference type="GeneID" id="59329884"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="273" mass="27995" checksum="B42C1F42139E70BA" modified="2022-01-19" version="1">MHRRTNSNDFFPLAKGISVASISTTGPAAASAIIILGNPGNGAQSSSETTSTSPSTSTTPTLTSTSTTKSAGGGVASQSPTSLVSVISIVSVVTSAADGAPVTTTNMIMTTIPAASVPASSNSNHHGAEIGGGVAGGVAFIALVSLAFFLLRRRKKRQQYQSAPTTEYRNSMDYMYKAKDMGSPNTAETSPEIDGTPIVGVYRGWGPTGQEGALRVVNGSDRQSTVSGLTGTNARDSLMSELESPTTPVRAGLRSHQPTIAEQPFELAGEGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBM7</accession>
    <name>A0A8H6FBM7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cell division control protein 48</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001511</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151860.1">
        <property type="nucleotide sequence ID" value="XM_037292441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBM7"/>
    <dbReference type="GeneID" id="59329927"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19528">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000004">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000001">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000003">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000048">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="7">
        <location>
            <begin position="45"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="6">
        <location>
            <begin position="145"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="257"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="531"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="825" mass="90329" checksum="781EB9C109627210" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBR3</accession>
    <name>A0A8H6FBR3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000623</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151923.1">
        <property type="nucleotide sequence ID" value="XM_037291561.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59329041"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051584">
        <property type="entry name" value="GPCR-associated_LMBR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355:SF0">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
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        <location>
            <begin position="559"/>
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        </location>
    </feature>
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        <location>
            <begin position="645"/>
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        </location>
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            <begin position="926"/>
            <end position="988"/>
        </location>
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            <begin position="1003"/>
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        </location>
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            <begin position="1132"/>
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        </location>
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        <location>
            <begin position="587"/>
            <end position="604"/>
        </location>
    </feature>
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        <location>
            <begin position="605"/>
            <end position="618"/>
        </location>
    </feature>
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        <location>
            <begin position="690"/>
            <end position="708"/>
        </location>
    </feature>
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        <location>
            <begin position="795"/>
            <end position="808"/>
        </location>
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        <location>
            <begin position="819"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="874"/>
            <end position="902"/>
        </location>
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        <location>
            <begin position="972"/>
            <end position="985"/>
        </location>
    </feature>
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        <location>
            <begin position="1007"/>
            <end position="1038"/>
        </location>
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        <location>
            <begin position="1059"/>
            <end position="1081"/>
        </location>
    </feature>
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        <location>
            <begin position="1133"/>
            <end position="1153"/>
        </location>
    </feature>
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        <location>
            <begin position="1217"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1267"/>
            <end position="1289"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6222577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1301" mass="143982" checksum="18DF4A95836830B9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8H6FCY9_9LECA</name>
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        <submittedName>
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        <name evidence="2" type="ORF">HO133_000349</name>
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        <name type="scientific">Letharia lupina</name>
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            <taxon>Pezizomycotina</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="31535" checksum="81E54881E5A8B23B" modified="2022-01-19" version="1">MDSKKGKKKAAGPAKNPRIITKCAEMEAKVQQIGELKVKFAGVAKALRPALAEMAGRTSQQMGHATYDKDGRRKAQYDALVVELEKVRGSNIARRVAYHETHRELKAVPVQHETLQEMNTIENQYRKKVDEIRESSLTHLRLSYMTRVVQIANGLGADLENLGQGSNKRTHRSPDPNGNNIARYKDTQASAKQHFYWSTKVMWDDWTNRKHADSEMASQGRILPRPYNYIQAEMIESGARGHRMELHSEREDKRPDSTWIGGMGKLAYFEAGEGNVGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDN3</accession>
    <name>A0A8H6FDN3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_011087</name>
    </gene>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
            <end position="479"/>
        </location>
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            <begin position="204"/>
            <end position="234"/>
        </location>
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            <end position="288"/>
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        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="479" mass="48703" checksum="8A2AA865484DB18F" modified="2022-01-19" version="1">MDLAFLTRLIGLALVALPVAYAYPSSLSSFISAPLSQPLSQATVTIAGQVFIVDPADIILDRTTIEPGSAGISINDQIVSADAAHDLFVDGTEILADPTGSNSAENIANNKTAKFSSNATFSGSVPASSVTAAAAGYDILGGEIAHIAVQVASTHATYRAGVPISTSVSASTSMAATPSGGSAPLGPVVIPSASSAMTNSSLSKASTAGLAPNVQENVSQTQSRSTGSDSMSASTPKINLIPTGSSIHSNSSSGLYVVAGASKISSSASSDRQSMHTTGTPSPSAILVPGGQPNTITTGPTLAIPTISLNPTGSVASSQVMLLVGAIFSITEEAKTLSAIIKDNGPKVSLVSKIKGIDDRILNELEDMHPPGPPPSNAAGPDFCAANSWIANVQIDDAVTNAPPYPTKDFKFAIPAGILEPAPLPCTWQPSPGNSQPGSMRCNPGEEIVLCTIPVTTAITCKQDNPDRIEPLAQCVFWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEJ2</accession>
    <name>A0A8H6FEJ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010353</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154023.1">
        <property type="nucleotide sequence ID" value="XM_037301206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEJ2"/>
    <dbReference type="GeneID" id="59338744"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF1">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="40"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="653" mass="73412" checksum="200D18200CD6DFEE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEL8</accession>
    <name>A0A8H6FEL8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153886.1">
        <property type="nucleotide sequence ID" value="XM_037301069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEL8"/>
    <dbReference type="GeneID" id="59338606"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="366" mass="44740" checksum="507E9FC7F04BA328" modified="2022-01-19" version="1">METSRGPIGGLALNIKFWNPFSSKNKEKKGKMQYYVVRKTKKSERGPAVIQQQPPIPPPPRTHFVPLVRGEHPVVIPMEPPERPPTHHPLGRERRRRSPVPAVIHQSSEEDEDESLSPLEANREHGRRTRSLSPNHRYHVEKERIRQRELRQREYQERIKREEREARERASILEHVHRQRAMEERQEQIRQQRREDRQRLANEREARRQQQLEEQARVVREQEDLERLRALARARILQEQEDLERLREAERARQLREERARRHQEEWRLRDEQARRQYEERQRYARAHQANIPRRPRHPVFVHPNEDRLDRGDRFIQNAIRAENLRQFERRAGWARGRYDDGALRRRNTIDGGQRWYDGQWRRERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEN5</accession>
    <name>A0A8H6FEN5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Stress-activated map kinase-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010289</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153960.1">
        <property type="nucleotide sequence ID" value="XM_037301143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEN5"/>
    <dbReference type="GeneID" id="59338680"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031567">
        <property type="entry name" value="CRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008828">
        <property type="entry name" value="Sin1/Avo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031313">
        <property type="entry name" value="Sin1_PH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335:SF1">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16978">
        <property type="entry name" value="CRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16979">
        <property type="entry name" value="SIN1_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIM" evidence="3">
        <location>
            <begin position="337"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="SIN1-type PH" evidence="4">
        <location>
            <begin position="692"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="511"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="806" mass="88982" checksum="75D44C142B761016" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFU1</accession>
    <name>A0A8H6FFU1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_009159</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154846.1">
        <property type="nucleotide sequence ID" value="XM_037300020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFU1"/>
    <dbReference type="GeneID" id="59337554"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="164"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="366"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6226293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1126" mass="123801" checksum="F29DB48C8AEF72DD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG16</accession>
    <name>A0A8H6FG16_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Maltase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_009370</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155057.1">
        <property type="nucleotide sequence ID" value="XM_037300231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG16"/>
    <dbReference type="GeneID" id="59337765"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000138">
        <property type="entry name" value="Putative alpha-glucosidase AgdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030459">
        <property type="entry name" value="Glyco_hydro_31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF133">
        <property type="entry name" value="P-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00707">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034965350" description="alpha-glucosidase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="12">
        <location>
            <begin position="166"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="11">
        <location>
            <begin position="340"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="13">
        <location>
            <begin position="773"/>
            <end position="865"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6226504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="996" mass="107914" checksum="E8B0DE64601E12E5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG75</accession>
    <name>A0A8H6FG75_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008806</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155670.1">
        <property type="nucleotide sequence ID" value="XM_037299669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG75"/>
    <dbReference type="GeneID" id="59337201"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="71"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="193"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6227362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="743" mass="84019" checksum="516885A8D3F88515" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG78</accession>
    <name>A0A8H6FG78_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 9">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1 9">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008397</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1 9"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155264.1">
        <property type="nucleotide sequence ID" value="XM_037299263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG78"/>
    <dbReference type="GeneID" id="59336793"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000001">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032005">
        <property type="entry name" value="TyrRSs_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF0">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="TYROSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16714">
        <property type="entry name" value="TyrRSs_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosyl-tRNA synthetase C-terminal" evidence="11">
        <location>
            <begin position="380"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="52"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="351"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6226956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="544" mass="61593" checksum="E968B82DC4FE1474" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH19</accession>
    <name>A0A8H6FH19_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007718</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155924.1">
        <property type="nucleotide sequence ID" value="XM_037298588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH19"/>
    <dbReference type="GeneID" id="59336115"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="169" mass="19154" checksum="18BC8367D4C90ACD" modified="2022-01-19" version="1">MEEKLDAEERETQITLLISKDGSTPGASTSQDLGSSWAEVIQVQYYPQQNQLPLKTERYHTIAVALGLARPLLPERRCILSRGVHGQLIDDLPNHDIDFAANMGTEYDSRFDQSLGLARSNHHREEVVYAQHRQDKMQKRLEGQQVQDAKAIERILRQEEGEGCMALGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHV1</accession>
    <name>A0A8H6FHV1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">eIF-2-alpha kinase activator GCN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008213</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GCN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156417.1">
        <property type="nucleotide sequence ID" value="XM_037299081.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336610"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904688">
        <property type="term" value="P:regulation of cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000090">
        <property type="entry name" value="eIF-2-alpha kinase activator GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022716">
        <property type="entry name" value="Gcn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056810">
        <property type="entry name" value="GNC1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056809">
        <property type="entry name" value="HEAT_GCN1_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF7">
        <property type="entry name" value="STALLED RIBOSOME SENSOR GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12074">
        <property type="entry name" value="Gcn1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24993">
        <property type="entry name" value="GNC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25801">
        <property type="entry name" value="HEAT_GCN1_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24916">
        <property type="entry name" value="HEAT_GCN1_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="1334"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1509"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="1652"/>
            <end position="1910"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1969"/>
            <end position="2006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2622"/>
            <end position="2651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2664" mass="290441" checksum="6B4857831DFD145D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJ35</accession>
    <name>A0A8H6FJ35_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007544</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157070.1">
        <property type="nucleotide sequence ID" value="XM_037298435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJ35"/>
    <dbReference type="GeneID" id="59335943"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF31">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN YCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="139"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="395" mass="41980" checksum="C2C7B1D23E697241" modified="2022-01-19" version="1">MSPFYSILSVWKPLTTTTLLTLSTLFTAPLVTANPTCTNLRIPLTATASNEVFPIPVGLNYSNPAAVSAFVQTVLGDAGTAYPLIPTTFTGIVAARYCEPEVTIANRSDVVQLFMSGVTENNLYWFGLGYPNGYDGNEYSTVDYASKQGYPTLAIDRIGVGNSTHPDPILQQQVNLEEAVSHQLVTMLKAGTAVPGKTFNRIIFVGHSYGSVLGNAQATNHPGDISAFILTGFGVSIIPVAADLPQTVPFPATLYAPRFAGFAPGYLVTSSEAGRRAYLWGKPGSFDEGIFEKDYDDEDVVGLGELLSIDGGLKEAPASTAPVYIITGDSDDVFCLAATCGDGPSSPQAQACALFPKAETCEYFIPVGTGHMISLHYSAQTSFQKYHAFLASVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJW2</accession>
    <name>A0A8H6FJW2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157099.1">
        <property type="nucleotide sequence ID" value="XM_037295098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJW2"/>
    <dbReference type="GeneID" id="59332588"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="255" mass="27539" checksum="FB4248DCA2CA39D9" modified="2022-01-19" version="1">MAQASWEGWIVHKYVIGLPGGTAVKITLTPTSTTGPPERPSSLQNGYAIVSLYDLGIAVAKENKFYTANCDIVVVGVKTGNIKVEERTEERTEEQQGALLEDGLTLVDGPLNLTDLSIPANHDLAALPVGPRSYSKGNFKIEYEYDGARISPTDIFTACLDGFATSAPHPSTDVAAAVSARSLSNDCAINMRGVGLPSRFTWDHVIRTFSILFVNIMLGLRAFGEMRFSIFLDSAQIGTGFVLKPQRLAQNGTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKB4</accession>
    <name>A0A8H6FKB4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial aspartate-glutamate transporter AGC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Aspartate-glutamate carrier 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_004449</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Calcium-dependent mitochondrial aspartate and glutamate carrier. Transport of glutamate in mitochondria is required for mitochondrial transamination reactions and ornithine synthesis. Plays also a role in malate-aspartate NADH shuttle, which is critical for growth on acetate and fatty acids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157367.1">
        <property type="nucleotide sequence ID" value="XM_037295366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKB4"/>
    <dbReference type="GeneID" id="59332857"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000004">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000415">
        <property type="entry name" value="Mitochondrial carrier protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF9">
        <property type="entry name" value="CALCIUM-BINDING MITOCHONDRIAL CARRIER PROTEIN ARALAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="97"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="169"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="345"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="444"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="542"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6230110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="699" mass="77568" checksum="0E2F07F75A9C504F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKI4</accession>
    <name>A0A8H6FKI4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004549</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157467.1">
        <property type="nucleotide sequence ID" value="XM_037295466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKI4"/>
    <dbReference type="GeneID" id="59332957"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051295">
        <property type="term" value="P:establishment of meiotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21223">
        <property type="entry name" value="CH_ASPM_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051185">
        <property type="entry name" value="ASPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="587"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="117"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="738" mass="82664" checksum="78355D45DDD0B7AD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKL8</accession>
    <name>A0A8H6FKL8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">HO133_004614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157531.1">
        <property type="nucleotide sequence ID" value="XM_037295530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKL8"/>
    <dbReference type="GeneID" id="59333021"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="136" mass="14597" checksum="6B7139E95A9A26F4" modified="2022-01-19" version="1">MPTSPIEVLLQRDMEWRADDVTKFAADISSRVGDADTLVGAPSIKDVEYKEKSDVNDADMLVGSQVVKEADIGSTVARKDEGKSQPSGLQRDVQAEVVTADGLAVEQRGGPFSGTEAAELFESEHPGVLFDLQCND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKQ5</accession>
    <name>A0A8H6FKQ5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157525.1">
        <property type="nucleotide sequence ID" value="XM_037295524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKQ5"/>
    <dbReference type="GeneID" id="59333015"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="263" mass="26637" checksum="BD448A09A67D350F" modified="2022-01-19" version="1">MEMQGTPIGSTDQGMKNTSDPERYTNPTSEGAGAIASDSLAAESASSGGKFGENRDSNPSSVKGDHSTLNNTDTSGAKVLAPASNAAEREEHEALEETPDEAKGSHGKKYPEGADGQSDIPGHSLDGKPASGLRGVTGPSSSMGTSGQMAGSENKSGPTENTFKGEGGADVDAAPGYVASVESEANRSGKPKGKNITEGGFDDDPSNNASFTSEIGSENDPGRKAEGDMQKMAQSASGGTGPRQAPGESDNSQYDVLDTDQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKX4</accession>
    <name>A0A8H6FKX4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004779</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157693.1">
        <property type="nucleotide sequence ID" value="XM_037295692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKX4"/>
    <dbReference type="GeneID" id="59333185"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006040">
        <property type="term" value="P:amino sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005338">
        <property type="entry name" value="Anhydro_N_Ac-Mur_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30605">
        <property type="entry name" value="ANHYDRO-N-ACETYLMURAMIC ACID KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30605:SF0">
        <property type="entry name" value="ANHYDRO-N-ACETYLMURAMIC ACID KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03702">
        <property type="entry name" value="AnmK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="197" mass="21841" checksum="9BAA79818F4AC82C" modified="2022-01-19" version="1">MDTVECVLCHFTQASPTSSPHLKILRYNAIDNPQSFSTDAGLSPEDTVATITRITAEAIVNAYYTWGPKDKEGKLDLEEVYMCGGEAFYPNTWDYVQQELGPNVRMTMLDESGVGGEAKENITFAFQATDAVLGRPLVVPQRVERKPSTIVGKVSPGRNYMELMRTSMAFGGSFEGDCLPPVKEMVLERWEGNHAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYT5</accession>
    <name>A0A8H6KYT5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_007436</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the complex I NDUFS4 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156962.1">
        <property type="nucleotide sequence ID" value="XM_037298327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYT5"/>
    <dbReference type="GeneID" id="59335835"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.190:FF:000001">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 21 kDa subunit mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.190">
        <property type="entry name" value="atu1810 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038532">
        <property type="entry name" value="NDUFS4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219:SF8">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="NDUS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="53"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6229320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="251" mass="28244" checksum="9854E09F08F0C061" modified="2022-01-19" version="1">MTATNPSSHCVRLTEDVSAGEMSLLRPQYASRLFQARIATHQRMPPSHIRWASKTSSGEYQDRSAQPAPKSDASTSPSPTSGDSSMIRQQDPGEAMVDHQPDYHAPIDHGTSQFSPIPKRVMNGSEPGDITPAAVLSGAPVDLQARTVRIYRPAKTATQSGDWHGHHWRMDWDILQKGHRWENPLMGWQSSADYLQGTHLNFASREDAIHFAQKQGYEYFVQEPNERRFVPKAYANNFMHSPKKLKKIPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYU4</accession>
    <name>A0A8H6KYU4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pre-mRNA-splicing factor SYF1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Pre-mRNA-splicing factor syf1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_007495</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in pre-mRNA splicing and cell cycle progression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157021.1">
        <property type="nucleotide sequence ID" value="XM_037298386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYU4"/>
    <dbReference type="GeneID" id="59335894"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000666">
        <property type="entry name" value="DNA repair and transcription protein (Xab2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000023">
        <property type="entry name" value="Pre-mRNA-splicing factor SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000038">
        <property type="entry name" value="Putative pre-mRNA-splicing factor SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056350">
        <property type="entry name" value="HAT_Syf1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23220">
        <property type="entry name" value="HAT_Syf1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="15">
        <location>
            <begin position="25"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor SYF1 central HAT repeats" evidence="13">
        <location>
            <begin position="188"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="14">
        <location>
            <begin position="403"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="761"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6229379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="834" mass="96748" checksum="E1F4140F0F62E827" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYY1</accession>
    <name>A0A8H6KYY1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157065.1">
        <property type="nucleotide sequence ID" value="XM_037298430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYY1"/>
    <dbReference type="GeneID" id="59335938"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="13722" checksum="15573498E0275D70" modified="2022-01-19" version="1">MADPGNGAERSLLAHILSASAFMDCGGEEEEGEEEGEGEEGEEGEEKKKREKKKEKKKEQKPPFPEQSQKLSTLLRQATKTPPSIRNPTWQMIARRELGPEGPDRDEQRTTHRSDCAYRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZQ7</accession>
    <name>A0A8H6KZQ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_004528</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">G proteins are composed of 3 units, alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G protein gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157446.1">
        <property type="nucleotide sequence ID" value="XM_037295445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZQ7"/>
    <dbReference type="GeneID" id="59332936"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000003">
        <property type="entry name" value="G-protein complex gamma subunit Ste18/GpgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041848">
        <property type="entry name" value="Ste18_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28189">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28189:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="11">
        <location>
            <begin position="19"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6230189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="91" mass="10458" checksum="6AB8242A5BB154F8" modified="2022-01-19" version="1">MPTAYEIRAGGDVKNKKQSMADLKLRRLNELNSRLREDLERPRIKVSEASMSLIQYCTNTKDFMVPSMWGSVDKREDPYAPQQSKGCCTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZS1</accession>
    <name>A0A8H6KZS1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157419.1">
        <property type="nucleotide sequence ID" value="XM_037295418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZS1"/>
    <dbReference type="GeneID" id="59332909"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17744">
        <property type="entry name" value="BRCT_MDC1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053036">
        <property type="entry name" value="CellCycle_DNARepair_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667:SF1">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="508"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="756" mass="82389" checksum="7C526009705D0E16" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZT1</accession>
    <name>A0A8H6KZT1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004573</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157490.1">
        <property type="nucleotide sequence ID" value="XM_037295489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZT1"/>
    <dbReference type="GeneID" id="59332980"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="407" mass="45817" checksum="FB944BD3F67BD3EB" modified="2022-01-19" version="1">MRKCFPTRRIPILRSWDHRGLRTLRRIVSRNPAVKQLSNAELLDIIHQDKENATSTATAQTNSDGLDSQKDDFLPQAASPLATRIHRLPQSPLTDPGLVAARIRHRAVKPLPSGDPSPFQLKLQKNPYAIALATPPRLCVLTGLRLPSYFQIPFGVSTHPKTGAPWHLPKLSTQVVSNPSDGKIPNGNPEGIPPSFLEPDASTTMESPMRTLSSTHFLATRQVLAHVSSLTPPHYNRLMPYRWRQDPSVRLSEIVWREDMDIFVLEILRRNVSETLSYLASRPAAYIAPCKSYDNINKHGQVAAVLWLRKDAEISIHDKSSTSDATPFNVRETGPPPYAMHYHRTRYVPYYNLAALLGPSQLNALQESQPDHYGDQFAVIKLKRTTVKVQLELWKLLGYTAQDRIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L020</accession>
    <name>A0A8H6L020_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004894</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157807.1">
        <property type="nucleotide sequence ID" value="XM_037295806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L020"/>
    <dbReference type="GeneID" id="59333300"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="30242" checksum="6A349F44BDFE75CE" modified="2022-01-19" version="1">MLRTARIRAVLVSLVREFNRSQPRPWYDDPRQGFKSLEDVTLTFINSILMVFPTVYIDDSIGSPSCLGAHPRREWRNFFHPREQSILLNGPRVRDMGTAAIIAARTGNPSDHANKRFRTFIFMFANTFFHEMAHMLVTFLTQDLTLTPPNIGPRISGYSNGGIGEAGRWLETTAFGGTLEYYRDHSDDGGQPGIPHILTEGGVRRISQHSIDDIVSYCDHYPPVNLTDPMRWIVMDEYRRHCQKVDVGPQSALAQLPFQPGLRAQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6R1</accession>
    <name>A0A8H6C6R1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147335.1">
        <property type="nucleotide sequence ID" value="XM_037297210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6R1"/>
    <dbReference type="SMR" id="A0A8H6C6R1"/>
    <dbReference type="GeneID" id="59334713"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="228" mass="26073" checksum="4B9EE64504D0D4B4" modified="2022-01-19" version="1">MATPRAGFHREFDPTFPSPDLTVCSLCGLDPCDWDSDNPFDFHATRSPECPFVRATQQKKKHRNQKTRQQYKRSLKATQSATQPEHSVEQEEESLQQANAENTAEKPSDNKATIIKNTAQLSKTPRHMLSTIPHQTTTQQENITAIWPIISAARLWHTITELSIQEKDLLRRSQPNHYGYGNWTSAGKGHIGRCIFGRIETVRCLASTSILLFERLSLRQENLWDSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Y0</accession>
    <name>A0A8H6C6Y0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006578</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147186.1">
        <property type="nucleotide sequence ID" value="XM_037297476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Y0"/>
    <dbReference type="GeneID" id="59334979"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="436" mass="50555" checksum="805A0E4A219A6E71" modified="2022-01-19" version="1">MDTQARNKIGVLKPEDWKKLGYSDSTTPNDDTEGLTNEIDPIFHYTHPSLSVPIWHPLTNAEYEDYLVDIRQMLQLASLFLRSPPSLNADYDLYYSPRVYPKKRVNFEGQPVLEFRHVETTEAYWPERRDLANAALDRLAGVVSFRVVSEKENPDMAGVIGLNTLFLGVHPRGVNIQDDTDLAKGIGSTVWIHERLITELRRLRNEKGVDNTLKVCSLTVKGAATICHELFHAKYMATDSPLLRDTLEETSLRRHAAMTKSGRQKEDVQSNEPLHEDDTDAEVGYVWEKYVFGGHIHFEGDVDKCLFFSKWPSYWASKNYHRRGLWRKMMTRYVVTMHYILNLLRQGFWDDMKEGDLTALQIKKWIGIRTSCPYSELEDPDWNINDSSEGEYPLVRPSLLRICREKAGEELQVSRANESREERIDRQLWEQTEARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7L6</accession>
    <name>A0A8H6C7L6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005729</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147817.1">
        <property type="nucleotide sequence ID" value="XM_037296636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7L6"/>
    <dbReference type="GeneID" id="59334134"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="130"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="446"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="508"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="522"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="570"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="584"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="607"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="617"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="646"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="670" mass="75191" checksum="DEBE8E7EFBFBCA9F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7V6</accession>
    <name>A0A8H6C7V6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005642</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147731.1">
        <property type="nucleotide sequence ID" value="XM_037296550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7V6"/>
    <dbReference type="GeneID" id="59334047"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051711">
        <property type="entry name" value="Stress_Response_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540">
        <property type="entry name" value="THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR RGLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="43"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="399" mass="43332" checksum="788D379B4B4AC6CF" modified="2022-01-19" version="1">MPTPTAPPIHKTLSACDECRTRKLKCSGEAAGCSRCKSESTTCHYSQKKTMGRPRKRRHEEAGGTDREENMGASEGLDVGAADVGPRDSHLATFSGNDPGLGTDGFHFSGNSLEEEDTFPFGPGNGSIFPDTDCFSFDLPEGPASNLEQGLPDWDPSQDLGQPFSTLADTPLRLQTPPNVPGKVDSNSCSTGNAVIGCSCLSSLYSMLAKFQSLPETSFPYSMGALRSAASLGRSIVACHNCLQAYNTALQNTMLLGALLQLLIIEYAKLLKHIEERSKQAEKIAFRFGDPSSPFDSRHTGRPDCPMAINIDLSGDEWRTLARKAVAQEVNNSQGSHGLFGLVQEMRDRQVSWHERFSKGQHTAFHCADHQQSAETSDNLCVQIMSIDNLKRSLETLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C843</accession>
    <name>A0A8H6C843_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147844.1">
        <property type="nucleotide sequence ID" value="XM_037296663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C843"/>
    <dbReference type="GeneID" id="59334161"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029497">
        <property type="entry name" value="Fimbrial_PilY2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14481">
        <property type="entry name" value="Fimbrial_PilY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="783" mass="78522" checksum="4D6739AD5BB2F06E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8F3</accession>
    <name>A0A8H6C8F3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CipC-like antibiotic response protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148194.1">
        <property type="nucleotide sequence ID" value="XM_037296212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8F3"/>
    <dbReference type="GeneID" id="59333707"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022234">
        <property type="entry name" value="DUF3759"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37450">
        <property type="entry name" value="CIPC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37450:SF1">
        <property type="entry name" value="CIPC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12585">
        <property type="entry name" value="DUF3759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15593" checksum="939E0F9667B47357" modified="2022-01-19" version="1">MVFGWGESQDAHDQVYNQGGGYDNPDNQAKFSHELVAGGAAFEGFKLYEDHQRKEGNVVNHAFAKELLAGFVGGEVDKLAETKGMNAYDRERAHHQAKENSSHMYDEHYVRGQNADQYDPNQYDAPQQFQGGYGGQGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8F6</accession>
    <name>A0A8H6C8F6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005947</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. TCR/Tet family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148031.1">
        <property type="nucleotide sequence ID" value="XM_037296850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8F6"/>
    <dbReference type="GeneID" id="59334352"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF12">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="451"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="522"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="72"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6218596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="549" mass="59022" checksum="0817CA231C7471B0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C904</accession>
    <name>A0A8H6C904_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148552.1">
        <property type="nucleotide sequence ID" value="XM_037295854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C904"/>
    <dbReference type="GeneID" id="59333348"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF25">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="92"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="241" mass="27015" checksum="6278D3B722B18E0F" modified="2022-01-19" version="1">MDYKLHPTGWSFSGPAGTAAQDPIYSFKRFSELYLKADSEYKGRYSVPVLCDREEETIVNNESPEIVRMIYSGFWVRFPAAVGDEVVGGLYSKGLRGKIDGMDGWVNSVLNTGVYKTGFATEQGNHEKACIEVFEALDQVGDILANSKGEYLFGEHITEADVRLYPTIVRFDTAHYTLFKCNLKIIRHDYPKIQRSLVNLYGQKGFGDTTDVEHIKKGYALTPRNNLVPLGPAPDMPLAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9D5</accession>
    <name>A0A8H6C9D5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148870.1">
        <property type="nucleotide sequence ID" value="XM_037294823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9D5"/>
    <dbReference type="GeneID" id="59332312"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="25666" checksum="D4A0E697A205D17D" modified="2022-01-19" version="1">MDGGLEVDGRNNLPIPYQKHGEDYKYYNGEEQHDQPSYETYSTEPLTKSKRLCGLRPATFFLSIALAVSVVLAVVAAGVAGSLAAKRGDTRTVAQSNSSSSIPASNPSCPSFNDTSTLPVYSYHINDSPFQPTSNCTSLGSTYTSLFTSASFKLQCGLDLPSNNLYSVFVYYFTDCLEACAAVARYPGLNQTCYGVAFDTNYDVTPEGQYGNCFLKDSPDITAKAANVNFTSSAVLVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9D7</accession>
    <name>A0A8H6C9D7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-tyrosine-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005094</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148704.1">
        <property type="nucleotide sequence ID" value="XM_037296006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9D7"/>
    <dbReference type="GeneID" id="59333500"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="101"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="267"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="52650" checksum="F11AC5E83FF4612C" modified="2022-01-19" version="1">MTAPFAKGFSTAYKRARSQDSPTPLASPPVLKATKIEEEEEEEEKEQGAQVESRIPAFLHQSQSAVRAKFNDLAWKEKLRILQGRSDPSSQWVQDTSPHVMRRNRYGNVQPWEKSRIHLKVAEGKSDYINASPISLRDPRTGVETKFIATQGPKQSGLSHFWHMIWQETTDVAVIVMLTQTHDGTTEKCFQYFPLNVEAGYFKVEPLDRARDTPEGSVTFLEYHLDMDSKTEIRKLSLRFGAETKDVWHFLFSGWPDFAVPEDNNRTALLELLKLCAEKNTSPSNPRIIHCSAGVGRSGTFIALEYLLAQVESGAILDAKEGEDMIYEVVNCLREQRMLMVQMEGQYQFLYDVIRDQFKERQLQASGQHSPKLQVQANGMKPAIIDDASNAGGFFSNVKTDESQAAAVAEMKSGEGQVEESKPAGQDPGVPWLAASFGRDFLFVSSCIVAFAAGISGMYLWFRGTGADGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9E1</accession>
    <name>A0A8H6C9E1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004916</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148526.1">
        <property type="nucleotide sequence ID" value="XM_037295828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9E1"/>
    <dbReference type="GeneID" id="59333322"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF35">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="16"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="396" mass="44755" checksum="51F980AA4D32A003" modified="2022-01-19" version="1">MADNATTAAIKPFKIAITDTGFHVVAPSIPGFGFSSCPNKTGLKHRQDAEVMHKLVSRLHYSEYVVQGRDWGAMIVWAIAHTYPKSAKALHVNLLSLTEPDYNYKPEYTEFEERSLRQHEHFDTKGFAYYLVQNTKPRTLGFAMHDSPVAMLAWMADKLFTWSDSYPWSPTEFITWTLLHYFPGPTGGFYIYRENSATELMSGADADKFWETPTAVSAFAKGAEMVPRSWAERKANVVFWQEHESGGHFAAYEKPEQLASAVGPQAPKVRPEDIPTYFPTKFGVLDGEAFMKWILEEVGKGSRTLAEGSYHVNPGPQAHIFSSKLASVTIWMDSVNLTMTEVGLTAFNFYECSDLTTYGPTVWWDTPGFSQATITEVKNTGNNNYKQYRVPSRRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9H4</accession>
    <name>A0A8H6C9H4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003914</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148883.1">
        <property type="nucleotide sequence ID" value="XM_037294836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9H4"/>
    <dbReference type="GeneID" id="59332325"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000076">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.990:FF:000002">
        <property type="entry name" value="mRNA export factor elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="486"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="730"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1037"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1044"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1072"/>
            <end position="1081"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1116" mass="122462" checksum="A5DBAB09375C7006" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9L3</accession>
    <name>A0A8H6C9L3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005021</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with histone H3 and histone H4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ASF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148631.1">
        <property type="nucleotide sequence ID" value="XM_037295933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9L3"/>
    <dbReference type="GeneID" id="59333427"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1490:FF:000001">
        <property type="entry name" value="Histone chaperone ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1490">
        <property type="entry name" value="Histone chaperone ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036747">
        <property type="entry name" value="ASF1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017282">
        <property type="entry name" value="Hist_deposition_Asf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF0">
        <property type="entry name" value="HISTONE CHAPERONE ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037759">
        <property type="entry name" value="Histone_Asf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101546">
        <property type="entry name" value="ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="196"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="221"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="286" mass="31400" checksum="53EA87EE08D71FC7" modified="2022-01-19" version="1">MSVVSLLGVKVLNNPAKFGDSYHFEITFECLEQLQKDLEWKLTYVGSATSSEHDQELDSLLVGPIPVGVNKFIFEADPPNLRRIPTSEILGVTVILLTCSYDGREFVRVGYYVNNEYDSEELNAEPPTKPDIDRVRRNVLAEKPRVTRFAIKWDTEDSAPAEFPPEQPEADIVEDDGVNYGAEEVELEEGLKAELAAAEAEKGEDEEMAGADDTAGVKEKEDDDSDAGSEDLEAESSGSDDEDAEEEEEEGDADDDMEMGEGEHAEGSNAGKQQALHQQPGAVMVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9N8</accession>
    <name>A0A8H6C9N8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005118</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148728.1">
        <property type="nucleotide sequence ID" value="XM_037296030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9N8"/>
    <dbReference type="GeneID" id="59333524"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF6">
        <property type="entry name" value="LETM1 RBD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="10">
        <location>
            <begin position="151"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6219293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="341" mass="39403" checksum="86042D5C92F62712" modified="2022-01-19" version="1">MLFRPSIRCRQPQSGPILFSPRFIISSSKTLYLPIRNYATPRECKSKPRVPLQRSQAASVALNPPSTTHPPPLSLPEKLPDLPRYKNYFRVGKAYGVFYKTGLKAIWTNYKLARALPNHIFSSGQAKVHQAVRHGLLSRADFQLIRRTRRDINKVPLFALIWLICGGFTPLIVIFFTGAVPRTIWIPKQVQKAREEAERRRSKSRKESVFLFAGPPRRSDIESMPDEPQRNALKSYAQSLGLYPAWWDRWIPTLVPTSLIKRRVYRRLGELEVDDFAIERDGGAGKMAGEEVRMACEERGIDILRKEERNLRMDLERWMERGEERDKNADEKIRYSIGTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA13</accession>
    <name>A0A8H6CA13_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149112.1">
        <property type="nucleotide sequence ID" value="XM_037295065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA13"/>
    <dbReference type="GeneID" id="59332555"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="293" mass="33174" checksum="187D2E190D6D71EE" modified="2022-01-19" version="1">MSDNESVDSSSSEEISPPWGTIPAEIYLLRYPPPPPPALGPQELIFLKAWRPLSIRHLATDPGQIGNVWLRTFYGPGSDAKHAALISHIDLDNAVDKEERLLDDRLLYGRLEGKWNGLLELLPELLVADWEGDERWLGYRRRIAKDVEAFRNAKKRFEHGAVAVGDVAEGTDELAEAMERLRAFLPEEEVQPRILEVLRSHVQAECVVAYLIVEDEEALEEEEEEGLLLLVFVDAFGRVVRSNRVCASEAEQMGGFWLEEAWGDIAEWQNGKTGSDYEDDEAWEALLKHEELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAN9</accession>
    <name>A0A8H6CAN9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Cyclase 1 superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149186.1">
        <property type="nucleotide sequence ID" value="XM_037294498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAN9"/>
    <dbReference type="GeneID" id="59331987"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34861">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34861:SF10">
        <property type="entry name" value="CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="41181" checksum="D5DFB6FF696AAF91" modified="2022-01-19" version="1">MVDGKSIAQSRLGQVKDFLTLNKTATTIPFDPNCTKFPQRKDVPQAPYAPPGVPTAWVWGETDFVGRLNLLTPTRVKAAAASEIKTGDIVSLNLPLNHPEVPAFARESFKHEIKTLAENIAYDDKYELNTQSGTQWDGFRHFGWIPAPTFYNNTHGEDIVGPKANHKCSIHHWSDHGIAGRGILLDYRSYAEKKGVALNSYEADRISYAELEACGRDQGIDIRPQAQGGDIQIGDMLFIRSGFIKTYYSKPVEERNQLALRHHELGENDGQRYAGVKQEDAMLDWLHDCYFATVAGDSPTFEAWPSNQEYYIHEYLLAMWGCPLGEMVDLEKLAQTCREKNRWTFFVTSAPANVPGGVASHVNGTAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAV2</accession>
    <name>A0A8H6CAV2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003223</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149527.1">
        <property type="nucleotide sequence ID" value="XM_037294146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAV2"/>
    <dbReference type="GeneID" id="59331634"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045122">
        <property type="term" value="P:aflatoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013700">
        <property type="entry name" value="AflR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF31">
        <property type="entry name" value="MONODICTYPHENONE CLUSTER TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08493">
        <property type="entry name" value="AflR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="436" mass="47475" checksum="8E394EF47EE7E128" modified="2022-01-19" version="1">MPDQSERPATPPKPPKLRESCDSCLIAKVKCSKARPLCGRCLANGAPCAYSPSSRSGKRNRNSAGVTKPINTTAAQNNRNKSLESPRSAALPQSASYLTRLMYPLLDDADRSLLDPNTGPLGGTTSPLLQVNPSIAAPMIDTNTTNGEDVFDSSDFLPTPPFHHGDFMDFMHISQSNPFDFSTAPVSPRPSPPDHIPSSPPWTTKENPYLRHNAYQSPLDMIPMNHASSSHTTPRHPPPPQSSTSNSSLNQTNGERAQTCDCFAACLSVLQSLHNHSALLSSTQQGSPPFDIVLSINREAMETCSTMLGCANCVSKSGRSISTMMLATIFGKVMSLYRAACFLRFGPSTSMQATAQLAFGAYTVTGENRQLLEIEILLLELRKVESILAVYSERFRNAQAEKDDETSVYNALTAYLDKNLHYIVDFLQMRKGGVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAW6</accession>
    <name>A0A8H6CAW6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="8">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_003681</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149291.1">
        <property type="nucleotide sequence ID" value="XM_037294603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAW6"/>
    <dbReference type="GeneID" id="59332092"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055207">
        <property type="entry name" value="POLR3C_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013197">
        <property type="entry name" value="RNA_pol_III_RPC82-rel_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008806">
        <property type="entry name" value="RNA_pol_III_Rpc82_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08221">
        <property type="entry name" value="HTH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05645">
        <property type="entry name" value="RNA_pol_Rpc82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22536">
        <property type="entry name" value="WHD_POLR3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase III subunit RPC82-related helix-turn-helix" evidence="11">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III Rpc82 C -terminal" evidence="10">
        <location>
            <begin position="170"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC3 winged-helix" evidence="12">
        <location>
            <begin position="519"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="394"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="188"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6219856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="679" mass="76827" checksum="6F6F84362200B198" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBL2</accession>
    <name>A0A8H6CBL2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149963.1">
        <property type="nucleotide sequence ID" value="XM_037293885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBL2"/>
    <dbReference type="GeneID" id="59331373"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="252" mass="28457" checksum="FBB9BF893D5E50E8" modified="2022-01-19" version="1">MNPTINAIPIGVSLDHPLGNLDELINRVRTLVQTTALNGTSRDDGIDRVLAGSTYDPIVPIFFRCTWPYGSQTRHKLIWMKPSIPEADFGREITRNFKFNETGGAVRQMPATFVGCSPFSSGEKHYVEARKRLNCEFDKEIGPTNQQHITMLAYTMSGFFEMFVEPLQDRAKCFLCRQPCLLRSESRDRISVLVMGQVITCAECAEKHEAVEIQRLGRKWSLAVMYSLAQQHVLASSLKYTLNLGTAEYDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBT7</accession>
    <name>A0A8H6CBT7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002992</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149994.1">
        <property type="nucleotide sequence ID" value="XM_037293916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBT7"/>
    <dbReference type="GeneID" id="59331404"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="354" mass="37014" checksum="6F907D468D6FBE6B" modified="2022-01-19" version="1">MTGERPTVDGVKLHVVGCVVRPSLAQLVSSQLTLRMIRIARLGVGVGREAMEAALRTEATLAAEMALATERTLDLDTEATLCAEAMLLIEAWLWIAAKISTRATLWAAATLSTDAALWTDSTLCTEATLALEADIIDAMSAALAATTALFDTSDIGVVVAVDDGPFVELPPPLATLDGISGSDNVANVFWVVGIPGGAVVEAPVTQTTTPAELVVISEVVTVVVPVKSSKPGMVCRVKVMDWPLMLSVISEGPDVSNASRSEDMGGVKLPVAEADDFGGGVMVGIDRKDDVVRDVAAKAMRMLVVVLGKPSVGGADGALVAGSWLPREVEMLLINDDEREELLGFDSSCGLLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBY4</accession>
    <name>A0A8H6CBY4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Polycystin cation channel PKD1/PKD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002866</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149869.1">
        <property type="nucleotide sequence ID" value="XM_037293791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBY4"/>
    <dbReference type="GeneID" id="59331278"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056337">
        <property type="entry name" value="LHD_YVC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052971">
        <property type="entry name" value="TRP_calcium_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056336">
        <property type="entry name" value="YVC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35859">
        <property type="entry name" value="NONSELECTIVE CATION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35859:SF1">
        <property type="entry name" value="NONSELECTIVE CATION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23190">
        <property type="entry name" value="LHD_TRPY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23317">
        <property type="entry name" value="YVC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="489"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="521"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="YVC1 N-terminal linker helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium channel YVC1-like C-terminal transmembrane" evidence="3">
        <location>
            <begin position="255"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="622" mass="71868" checksum="6E1F0A247A68F691" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC78</accession>
    <name>A0A8H6CC78_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150056.1">
        <property type="nucleotide sequence ID" value="XM_037293978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC78"/>
    <dbReference type="GeneID" id="59331466"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="47583" checksum="9D0B57CF267E6A63" modified="2022-01-19" version="1">MGPESTALTTDVGSESPYQLDKVQTLRASSALLKHIKTEVQKKETVSGTKNLLAPENSSEDVFPSDVEPIWLVLTTKKFMTDQKRLKPRKVLLPHSLHTSSNTSICLISPEPQRQFKDAIAHPSFPTTLSKRITKVISIKKLEAKYRSFESRRQLRDSYDVFLADDRIVTYLAKFLGKTFYNTTAKRPIPVSLEASKPKERKNAALPSTKVRKEPSDTKSIAAPPLLAKEIERSLSTAQINLSPSTTTAVRVGLASYTPEQLAANIEAVMASLAGKLVGWKNVRAAHVKGPNTMALPIWLAEELWVDEGYLLEDEEAAEAKAKAAQKGKRKRKLIEGKEAGHADGAGKRKVDGESETADEPKQKKVKKLNDEDLSQEMKERREKLRQQKRQARDSVEGNDVTMDRTSVAKPKVKKTRRATTAISAFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD37</accession>
    <name>A0A8H6CD37_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002804</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150558.1">
        <property type="nucleotide sequence ID" value="XM_037293729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD37"/>
    <dbReference type="GeneID" id="59331216"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="102" mass="11412" checksum="88E589863C30F3FF" modified="2022-01-19" version="1">MVVQIVKESTWPSKRLKNFPATLKRLLRKRIGPITGDFNKFFEPDETREDNEVGNFALPSRELRADSLEDYIKGVWVVDPSASSLGATVNTKEGKSCAQATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD85</accession>
    <name>A0A8H6CD85_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M12B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002177</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150757.1">
        <property type="nucleotide sequence ID" value="XM_037293104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD85"/>
    <dbReference type="GeneID" id="59330591"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04271">
        <property type="entry name" value="ZnMc_ADAM_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034028">
        <property type="entry name" value="ZnMc_ADAM_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF159">
        <property type="entry name" value="ADAM METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13688">
        <property type="entry name" value="Reprolysin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034534282" description="Peptidase M12B domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="5">
        <location>
            <begin position="274"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="588"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="594"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="612" mass="65782" checksum="734A2CBFBEED21CC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDQ2</accession>
    <name>A0A8H6CDQ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_002386</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150965.1">
        <property type="nucleotide sequence ID" value="XM_037293312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDQ2"/>
    <dbReference type="GeneID" id="59330799"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16457">
        <property type="entry name" value="RING-H2_BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12717">
        <property type="entry name" value="RRM_ETP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011422">
        <property type="entry name" value="BRAP2/ETP1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034931">
        <property type="entry name" value="ETP1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047243">
        <property type="entry name" value="RING-H2_BRAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007:SF7">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07576">
        <property type="entry name" value="BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="336"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="UBP-type" evidence="8">
        <location>
            <begin position="369"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="277"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="650"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="521"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="670"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6221530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="681" mass="76063" checksum="BCF3E2A0C1EBAE54" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDR3</accession>
    <name>A0A8H6CDR3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001698</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151165.1">
        <property type="nucleotide sequence ID" value="XM_037292626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDR3"/>
    <dbReference type="GeneID" id="59330112"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="208" mass="23483" checksum="7E278EA1B2B4A9C7" modified="2022-01-19" version="1">MTDSEVNLISREDLMLIWLETWETNAEPPQSTLAAQLVSHFTDGKKHPRTQDVETFRQLLREILGTESGQIFHAEPLETDSDVDCKLIYVIVKAGLEKISSDDPFNGKTELSRHAADSLTAINFTIKRNPEVLFAAPQFHGPDPRPIGPLYLWLLPKLLALTGCLQDSETIEGVLRIFTTFLVTERKTHVRRMNLHPVLTYMKGCTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE67</accession>
    <name>A0A8H6CE67_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151024.1">
        <property type="nucleotide sequence ID" value="XM_037292485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE67"/>
    <dbReference type="GeneID" id="59329971"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034411785" evidence="2">
        <location>
            <begin position="27"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="562" mass="57565" checksum="615CCF0EDCD29C34" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE93</accession>
    <name>A0A8H6CE93_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Bifunctional cytochrome P450/NADPH--P450 reductase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="14">Cytochrome P450</fullName>
                <ecNumber evidence="14">1.14.14.1</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="14">NADPH--cytochrome P450 reductase</fullName>
                <ecNumber evidence="14">1.6.2.4</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_001572</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24040"/>
            <dbReference type="Rhea" id="RHEA-COMP:14627"/>
            <dbReference type="Rhea" id="RHEA-COMP:14628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="1.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17149"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:142491"/>
            <dbReference type="EC" id="1.14.14.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <cofactor evidence="14">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14 15">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">In the N-terminal section; belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="14"/>
    <dbReference type="EC" id="1.6.2.4" evidence="14"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151041.1">
        <property type="nucleotide sequence ID" value="XM_037292502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE93"/>
    <dbReference type="GeneID" id="59329988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070330">
        <property type="term" value="F:aromatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06206">
        <property type="entry name" value="bifunctional_CYPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11068">
        <property type="entry name" value="CYP120A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000040">
        <property type="entry name" value="Bifunctional cytochrome P450/NADPH--P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023206">
        <property type="entry name" value="Bifunctional_P450_P450_red"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF127">
        <property type="entry name" value="BIFUNCTIONAL CYTOCHROME P450_NADPH--P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000209">
        <property type="entry name" value="Bifunctional_P450_P450R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 14" id="KW-0274">FAD</keyword>
    <keyword evidence="5 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 14" id="KW-0288">FMN</keyword>
    <keyword evidence="4 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="12 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0521">NADP</keyword>
    <keyword evidence="11 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="17">
        <location>
            <begin position="498"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="18">
        <location>
            <begin position="673"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="473"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="473"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="485"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000209-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6221606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1064" mass="118788" checksum="ACA5BCB5BA937251" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CER8</accession>
    <name>A0A8H6CER8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001309</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151658.1">
        <property type="nucleotide sequence ID" value="XM_037292239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CER8"/>
    <dbReference type="GeneID" id="59329725"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1050:FF:000003">
        <property type="entry name" value="Decapping enzyme Dcp2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000003">
        <property type="entry name" value="M7GpppN-mRNA hydrolase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140586">
        <property type="entry name" value="Dcp2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="10">
        <location>
            <begin position="90"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="384"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="497"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="537"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="621"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="923"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="389"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="555"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="711"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="764"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="930"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="960" mass="104339" checksum="C6A803E941CF2001" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CER9</accession>
    <name>A0A8H6CER9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151346.1">
        <property type="nucleotide sequence ID" value="XM_037292807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CER9"/>
    <dbReference type="GeneID" id="59330293"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="135" mass="14874" checksum="F88432218D23FB01" modified="2022-01-19" version="1">MDHPSKQRLFFPSKKPTAKAQENHHLHTFFSITLELKNARKASNICTPIIAAAANGSIKYSIRTNAFCSIERGKGGAAGCISRRSRRLMEENTLRLLQQQWRWVFASCGLVGDGNDEDAALGWGTGSKGEAKKDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFM1</accession>
    <name>A0A8H6CFM1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001427</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151776.1">
        <property type="nucleotide sequence ID" value="XM_037292357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFM1"/>
    <dbReference type="GeneID" id="59329843"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018791">
        <property type="entry name" value="UV_resistance/autophagy_Atg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157:SF5">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10186">
        <property type="entry name" value="ATG14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="77"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="570"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="587"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="620" mass="69510" checksum="D58045096AF2B8B4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFT6</accession>
    <name>A0A8H6CFT6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152035.1">
        <property type="nucleotide sequence ID" value="XM_037291673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFT6"/>
    <dbReference type="GeneID" id="59329154"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="192" mass="21685" checksum="E95376693B1373F6" modified="2022-01-19" version="1">MFHKHLDQASKRYKEHPNVAAMYTSLPKDKAHRPQRAPTQDYPPKIRDFAYDKHESETNTRDFIAKFADFGFRSHHRRVNNGDLLSKAGVLGYEKKRQLQDPSVGLKAQFPGCFDMDYHPPQRQERPVSETTTTTATNSVSSDLSGTTDTPSLATSATMESLEEGSNGRRPRGIRDNPLILAVAVCMLEYMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFZ7</accession>
    <name>A0A8H6CFZ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alcohol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000745</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152044.1">
        <property type="nucleotide sequence ID" value="XM_037291682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFZ7"/>
    <dbReference type="GeneID" id="59329163"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08284">
        <property type="entry name" value="FDH_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42813:SF2">
        <property type="entry name" value="DEHYDROGENASE, ZINC-CONTAINING, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02810)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42813">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="7">
        <location>
            <begin position="38"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="6">
        <location>
            <begin position="169"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="37139" checksum="CD1BE3175D999306" modified="2022-01-19" version="1">MAKDTMKAVIFKEPHVVAIEDRPIPKIKEPTDVIGHQTSPTGFIMGHEFTGTVEETGSDVKNFKKGDQIVSPFTVSCGECFYCKQGHSSRCAKGALFGTAGLDGAQAEYVRVPLADSTAMKAPEGIADNALVLMADIFPTGFFAAHNAFKDLTKEQISEATVVLVGCGPVGLCALINAENYKPKHLLAVDSIQSRLDLAESLGAEPWNFQTDREGLDKRVKDLTDGRGADVVMEVVGMSHALMMGFELLRPWGIISSVGVHNAEIPWSGNQAYGKNLRIQMGRCPVRSIFPQALDMLKQKQHLLGFMSDKIMPMSEAVEGYDLFDKMKVQKDLVVFEAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFZ9</accession>
    <name>A0A8H6CFZ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152110.1">
        <property type="nucleotide sequence ID" value="XM_037291748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFZ9"/>
    <dbReference type="GeneID" id="59329230"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06503">
        <property type="entry name" value="ATP-synt_Fo_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47842:SF3">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47842">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="758" mass="84083" checksum="27112F63E7A48F7D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG58</accession>
    <name>A0A8H6CG58_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000903</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152198.1">
        <property type="nucleotide sequence ID" value="XM_037291836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG58"/>
    <dbReference type="GeneID" id="59329321"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF32">
        <property type="entry name" value="FAD-LINKED OXIDOREDUCTASE AFOF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="53"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="495" mass="54406" checksum="CA51ACDBFFD8A3B7" modified="2022-01-19" version="1">MVSGASLVQAILSFSVAKPANLSELFGPSLSPEAQIILPTDTDWSTQVQLRWTNYRAPKYLGAIKPATETDVQRIVKIADANKIPFLTTGGGHGISDYSPFKGIAIDLGNFKSVELDVSTNHLTIGGATEYSQLHDLLYDAGKELTLASCPCVGVLGATMGAGFGPLKGHRGLLLDALESVRMVTADGRLVTASARQNPGLFWAVRGAGANFGVVTSATFKVYDITNKGQVMVADLVYPATANHTFWRTLQSFEHAMPSRLALTAVAVYNRTIDLPLIVLHAVYFGPQEDGEAYLEPFKDLGPLVSNIAMIPQNTMFPSDHGTCVPNQRINLYTLALKQFDPPTFDSFYARLTDFWREYPDYQGRLLIQRFSNDAVAAVPDAETAYPWRDAIAHMNIESFYTDPGNDDAIDDFVTSARARFAETSGFDTLATYVNFAHGDEGPTAWYSARKLQKLSALKRQWDPKGLFSWHNPVPMRWDLDISEKEHLGNQKLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG60</accession>
    <name>A0A8H6CG60_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000935</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152230.1">
        <property type="nucleotide sequence ID" value="XM_037291868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG60"/>
    <dbReference type="GeneID" id="59329353"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF107">
        <property type="entry name" value="ANNEXIN ANXC4 (AFU_ORTHOLOGUE AFUA_3G07020)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0041">Annexin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="317"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="335"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="742" mass="83715" checksum="2514321E20C496F0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGL1</accession>
    <name>A0A8H6CGL1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000917</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152212.1">
        <property type="nucleotide sequence ID" value="XM_037291850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGL1"/>
    <dbReference type="GeneID" id="59329335"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42034">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42034:SF1">
        <property type="entry name" value="CONDENSATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="452" mass="50564" checksum="21AA41C700AD44FA" modified="2022-01-19" version="1">MAWTADTPGTFRKELGGLEKIYRFMSTAFKHTGQEHWGLYTVCAVDFGPILHSPAKALRDAWKALRFEFPALALTLDGYDAVYTLPTADIVEEWAQKTFIVEQNQQPDEIIVDYPLRDLPALYYLPATSEVLFLASHWRVDAAGCCLLLDRLFALVVQQPDIASLQWGPDLHKISPSLEDAAGSPEATSPEIEDMAQRVTSNFEQKAIRSIGLSYKGDRTTPPARPANRSIVLTPESSEALIKACKERQISVSAVIYAALAEIMFALTSIDGATEYSTIMAVNMRPYLKAPFNSPAHSVRTYVSSITPAVRRDSTFRERTVTLTQYFKTWHTNEFSQALREIYKRSSRALLTAPRPPGPPPNPPSGITLSSLGVIDHFLTGEYDGAVSVKRFRFGVSMLNRQMLLYLWTFRGQVHLSINYNDAYYDASTPQEVLDRIPATLETELGVRLETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH00</accession>
    <name>A0A8H6CH00_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000194</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152569.1">
        <property type="nucleotide sequence ID" value="XM_037291134.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59328613"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022124">
        <property type="entry name" value="DUF3659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057827">
        <property type="entry name" value="WW_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39461">
        <property type="entry name" value="LEA DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G04920)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39461:SF1">
        <property type="entry name" value="LEA DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G04920)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12396">
        <property type="entry name" value="DUF3659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23518">
        <property type="entry name" value="WW_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="1237"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1367"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1389"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="549"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="631"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1412"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1572"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="549"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="631"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1412"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1434"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1584"/>
            <end position="1598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1623" mass="180182" checksum="7AE6135EF336DE0E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH77</accession>
    <name>A0A8H6CH77_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152532.1">
        <property type="nucleotide sequence ID" value="XM_037291097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH77"/>
    <dbReference type="GeneID" id="59328576"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034970471" evidence="2">
        <location>
            <begin position="22"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="416" mass="45240" checksum="50A1CD3CC9A11256" modified="2022-01-19" version="1">MTWMWTLIVLSWVFFFNACVAASLDICPLNSNDTVTTSSNITWAQAVDQPIRQLDGPSYLRPDIGGALVPWVYSIIILLVHIPVVIIRVVKWEAVQTWCLGATVFTIAITAQAFASTNFRSDKILTWTPLLLVIDAGSMAQVLFLVVEDYELLTRLRRAIMPLRVKPSEEPFLHDEEAGKSGDPDEGVFIAMRTVSPVASTTAPTSSTHPPLFTSKPLYVALISSLLLISVFALQFLGLHHAVNAARQSAPSVSWCSPIFQPFGVAVLDGNCNVWPILQTFSKGVGCILIPGNQQMAWLKATVAGTGISIALELVDIGILATVHSGTRWRGVKMRRPWFTMFSGVAVLGLILVFGVVYSTILPPGISEKVWIVMDAGAVTVYEGELGTAGLRGAMIGWNDGVFQGWGNTYFGSWAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHK6</accession>
    <name>A0A8H6CHK6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Purine nucleoside permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000248</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleoside permease that transports adenosine and guanosine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NUP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152623.1">
        <property type="nucleotide sequence ID" value="XM_037291188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHK6"/>
    <dbReference type="GeneID" id="59328667"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009486">
        <property type="entry name" value="Pur_nuclsid_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38643">
        <property type="entry name" value="PURINE NUCLEOSIDE PERMEASE C285.05-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38643:SF1">
        <property type="entry name" value="PURINE NUCLEOSIDE PERMEASE C285.05-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06516">
        <property type="entry name" value="NUP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013171">
        <property type="entry name" value="Pur_nuclsid_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034426260" description="Purine nucleoside permease" evidence="3">
        <location>
            <begin position="23"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR013171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="42857" checksum="426AAEA353B5A6C1" modified="2022-01-19" version="1">MRSISCTSQLLAAFASLSLAAAKPLSVLEARGHRHIKPKVFLIDMFPPEGAAWYGIPEFNLLAKNISVPGLSPLYPDVHCTEEGDVCQIVTGEAEINAANSMNALTFSGLFDFTKTYFMIAGIAGVSPKVATLGSVTFAKYAVSVELQYEFDAREIPANFTTGYVPQGSTDPIEYPQSIYGTEVFEVNDNLRQLAIGFAKTAVLNDSTAAMAYRANYASTSAYEAGSRGPSVVGCDAATSDVYFSGTLLSEAFENTTKLFTNGSGVYCTTAQEDTATLEALLRGAIANLTDFSRIIVMRTASDFDRPYAGESDTFNLFYANQGGFEPAIDNIYLAGIKVVEGILNEWESTFAKGIPATNYIGDIFGSLGGMPDFGPGSMFNDNPVKKRSPKGRNAARGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHL3</accession>
    <name>A0A8H6CHL3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152785.1">
        <property type="nucleotide sequence ID" value="XM_037291350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHL3"/>
    <dbReference type="GeneID" id="59328830"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF10">
        <property type="entry name" value="ABC TRANSMEMBRANE TYPE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="37769" checksum="8F12D9575211E4EA" modified="2022-01-19" version="1">MATTTESIAVEPYLQASNYGDDPDSAYGDEVGSYTTSLASNITDYKIENNRRYHAYKEGSYLYPNDEKESDRLDIMHKLTEVSLGGKLNLAPVPSNLQRILDIGTGTGIWAMEMGDKYPNAEILGNDLSAIQPRWVPPNVRFEIDDVEANWTYHQTFDYIHCRFMGSAIRNWPRLVRQCFEHTKPGGWVECIDLDLEWNSPDGSLTPDLASKHFNTTFLAASREGGMEPCPGPLLEGWLRDAGFKDVAAERHVWPVGTWPADRYLKEVGAWNYLQIMEGLEAFTYALFTRVLGYSQQEVDVVCAKIRKEMKNPKMHAYFYLHVAYGKKPEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIB7</accession>
    <name>A0A8H6CIB7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Guided entry of tail-anchored proteins 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GET1</name>
        <name evidence="12" type="ORF">HO133_010601</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WRB/GET1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIB7"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000006">
        <property type="entry name" value="Protein GET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03113">
        <property type="entry name" value="Get1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028945">
        <property type="entry name" value="Get1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027538">
        <property type="entry name" value="Get1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650:SF1">
        <property type="entry name" value="GUIDED ENTRY OF TAIL-ANCHORED PROTEINS FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650">
        <property type="entry name" value="TAIL-ANCHORED PROTEIN INSERTION RECEPTOR WRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04420">
        <property type="entry name" value="CHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034979840" description="Guided entry of tail-anchored proteins 1" evidence="11">
        <location>
            <begin position="21"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="4"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="173"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="23890" checksum="F345B1FA127C9616" modified="2022-01-19" version="1">MPSLLLLVFVLQLFIHLVNTVGAPVVNEFLWSLYNRLPTSTSADAKSQIKLQREVIRLKREMNATSSQDEFAKWAKIRRSHDKILAQYDEKTNNLKSFKSTFDRTVNALRWVGTNGLRFFLQFWFSKRPLFWIPRNWVPGYVEWLLAFPRAPTGSVSIQLWWIACATIVRLVGAAVVAALVLVREQEQGGQKMKIGAGGRGGKGEGKKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIE8</accession>
    <name>A0A8H6CIE8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Vacuolar protein sorting-associated protein 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010717</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the VPS29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153203.1">
        <property type="nucleotide sequence ID" value="XM_037301569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIE8"/>
    <dbReference type="GeneID" id="59339107"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07394">
        <property type="entry name" value="MPP_Vps29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000024">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028661">
        <property type="entry name" value="Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="196" mass="21184" checksum="7693F68D5718F45F" modified="2022-01-19" version="1">MTSRLVLVLGDLFIPDRAPFKKLLAPNKIGQILCLGNLTDKETYDFLRGIAPDLQIVKGDFDVEAPNLALSKVVTHGSLRIGFTHGHTIIPPGDADSLLIAARHMDVDVLLWGGTHKFEAYELEGKFFVNPGSATGALGTGWWAEGEEPVPSFCLMDVQGDVLVLYVYQLKTDASGVETVGVEKVSFRKQNSGSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIF2</accession>
    <name>A0A8H6CIF2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">propanoyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.176</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Propanoyl-CoA C-acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010519</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.176" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153005.1">
        <property type="nucleotide sequence ID" value="XM_037301371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIF2"/>
    <dbReference type="GeneID" id="59338909"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000016">
        <property type="entry name" value="Non-specific lipid-transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006102">
        <property type="entry name" value="PRK08256.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF1">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="10">
        <location>
            <begin position="25"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="11">
        <location>
            <begin position="270"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6223945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="458" mass="49990" checksum="70472C43D426759D" modified="2022-01-19" version="1">MGKKPVSAAYVLGVGLTKFIKPRGKVDYTELGFEAGIKALLDAQINYDDVEQGIACYCYGDSTCGQRVFYQFGMTQIPIYNVNNNCSTGSTGLHIARNMVRHGMADCVMVVGFEKMFPGSLQSFYKDREDPCRTTGEMMRVTRGRTDAPGAAQMFGNAGREYMEKYGANASDFAEIARINHLHSAKNPYSQFRDVYTLDQILSSPTIHSPLTKLQCCPTSDGGASALVVSEDFLAARPHLRSQAIQIAGQHLATDRPDLFDHSAINLMGFGMTQHAARTALAEAEVDPKDVRVCELHDCFSANEMITLDALGLCDPGKAHEMVRAGDITYGGRCVVNPSGGLISKGHPLGASGIAQCAELVWQLRGWANNRLVENTKVALQHNLGLGGAAVVTVYKRADGKTNEVVQDRDVKSKTWVGYNPAVEAKGFTKAQVDKVRSKKSRSEWALQDTEKKVEARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIH6</accession>
    <name>A0A8H6CIH6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010673</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153159.1">
        <property type="nucleotide sequence ID" value="XM_037301525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIH6"/>
    <dbReference type="GeneID" id="59339063"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="345"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="163"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="235"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="521" mass="57645" checksum="A1D5AB794FEC1ABA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIP3</accession>
    <name>A0A8H6CIP3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Shugoshin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010779</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shugoshin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153264.1">
        <property type="nucleotide sequence ID" value="XM_037301630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIP3"/>
    <dbReference type="GeneID" id="59339169"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011515">
        <property type="entry name" value="Shugoshin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011516">
        <property type="entry name" value="Shugoshin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07557">
        <property type="entry name" value="Shugoshin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07558">
        <property type="entry name" value="Shugoshin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shugoshin N-terminal coiled-coil" evidence="11">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Shugoshin C-terminal" evidence="10">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="162"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="269"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="600"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="288"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="401"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="417"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="480"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="617"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="742" mass="80688" checksum="A76A1465C202CD9E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ70</accession>
    <name>A0A8H6CJ70_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010761</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037301612.1"/>
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    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="AN11006-like"/>
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        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
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        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF24864">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="327" mass="37472" checksum="3A2EB37F716E9F8D" modified="2022-01-19" version="1">MAPSKRPSLEMAVTSSTLFGLPLEIRIMIYTQLLHSTAEILIPSNQFKRQRNCKGVGIYKHCALCGRSFGRIESYMSHQYLGCGQSPNFTRWPKGPEQAPSLWPALLRTCRFIHREAAPVLYRSNTFSFEDAATPNFFRWSTDQAQAPFIERVHVLMPFTRHIIPRKRETYHCRNPWWQYCMGNTFSLATDFPHLKGITITLSRGLAVAGSQDVRTNFELFAKHVYRLNWLQVIGLNDPTLLKYLYSVVERADEDHGRKGMQTEITEYGECIGWKNAIIWWGLPGEEAPCKPTPYAGDQRVRNRLFRIVDGNVVSYAAGQSFLDTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK21</accession>
    <name>A0A8H6CK21_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009994</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153667.1">
        <property type="nucleotide sequence ID" value="XM_037300850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK21"/>
    <dbReference type="GeneID" id="59338386"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="494" mass="53842" checksum="F01977CF1B14F000" modified="2022-01-19" version="1">MSTPTQTRPVITLYPSPQEDVSAYLAAIRLASSVRNTMDAQKAGVDGEKAAVYADSWGRHHPNFPPPPSPTPGGREPVQILNETSPADFASNTTKPGFFRPRDRYPSPSRSMSTGTWPFVEEHASAAAANLSTLADSPTGATLEALKRHHGHFPPPDPPMSTGTGSILEDEAADTPAGVKLEALEQHHGLFPPPDPPMSTGTGPILEDETADTPTGIKLEALEQHHGNFPPPEPSIGSTQAPPGKPISRRTGPIPIHQSNGPSGMKLYIPGEIPPQCQLYATNTDVNNVTFAVCPVTKRTINPTHNLASRQLHLADVSKSHILAIRDTAMCEAPTGFDERLACGKFDGWFVLAFFILFSMIGLAWIKLRKHKTRRLDEEHGTATWPRLSKVSTKSSEAKLKPQALGISTNRSEAKLRQKIRKAHEEDRHLDEELSTEQYESVAWRNASHRAARANFGGSVEHDAGPVAMLPELPAELPAYLFEPLYWLFGLERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKL5</accession>
    <name>A0A8H6CKL5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153803.1">
        <property type="nucleotide sequence ID" value="XM_037300986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKL5"/>
    <dbReference type="GeneID" id="59338522"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09998">
        <property type="entry name" value="HDAC_Hos3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000011">
        <property type="entry name" value="Histone deacetylase HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053244">
        <property type="entry name" value="HDAC_HD_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="2">
        <location>
            <begin position="251"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="646"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="726"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="811"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1061"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="729"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="787"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="908"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="964"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1038"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1076"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1110"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1158"/>
            <end position="1171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1196" mass="128772" checksum="3527DC029D749668" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKP5</accession>
    <name>A0A8H6CKP5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154100.1">
        <property type="nucleotide sequence ID" value="XM_037301283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKP5"/>
    <dbReference type="GeneID" id="59338821"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="173" mass="18967" checksum="D2780E080BBAF9F3" modified="2022-01-19" version="1">MSAASYYDKAATQEETSHLSSTSGQQPGPMHSGTQGYGQDMREGMNEQQQQRGVPQEGYNQQSGNGIYNGQEPNMGNTNYAHGAQQMNGNNNNYAQGAQQMEGNGAQGAVADDRDTITKLLDRIEKKFGGDRFNNPENAEKNKELNANILKRVKQVMGMVMQRGPGMLMNMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKV1</accession>
    <name>A0A8H6CKV1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Non-haem dioxygenase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010402</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037301255.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59338793"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DIOX_N"/>
    </dbReference>
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        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-haem dioxygenase N-terminal" evidence="2">
        <location>
            <begin position="32"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="144" mass="15606" checksum="FFCB1C71A00B73B8" modified="2022-01-19" version="1">MPTTESFAKSLPFPDDLPTVTHQRLELSKLLSGDEADSETLFEACASLGFFLLDLRGCTEGETILKETEAGFNIGQDFYALSGAEKSKFPLLPSKLGYKPIGGTKIEDGRPDRCEIDSLPTDDLLAFVPPNSNPPALREEPGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLR1</accession>
    <name>A0A8H6CLR1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154482.1">
        <property type="nucleotide sequence ID" value="XM_037300634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLR1"/>
    <dbReference type="GeneID" id="59338170"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42023">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42023:SF1">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="467"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="649" mass="71331" checksum="75C7C54EA2FCC09E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMY2</accession>
    <name>A0A8H6CMY2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154913.1">
        <property type="nucleotide sequence ID" value="XM_037300087.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337621"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057912">
        <property type="entry name" value="OB_CYT4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056625">
        <property type="entry name" value="SH3_CYT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056624">
        <property type="entry name" value="WH_CYT4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF65">
        <property type="entry name" value="EXORIBONUCLEASE CYT-4, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G01550)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25522">
        <property type="entry name" value="OB_cyt-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23214">
        <property type="entry name" value="SH3_CYT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23216">
        <property type="entry name" value="WHD_CYT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="2">
        <location>
            <begin position="903"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1414" mass="159832" checksum="7668693075BF3F10" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPQ7</accession>
    <name>A0A8H6CPQ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008796</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155660.1">
        <property type="nucleotide sequence ID" value="XM_037299659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPQ7"/>
    <dbReference type="GeneID" id="59337191"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06609">
        <property type="entry name" value="STKc_MST3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000499">
        <property type="entry name" value="Serine/threonine-protein kinase KIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="294"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="376"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="520"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="547"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="576"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6227352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="716" mass="79261" checksum="52B7BE9E66BF9161" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPZ2</accession>
    <name>A0A8H6CPZ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008902</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. PCH2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155766.1">
        <property type="nucleotide sequence ID" value="XM_037299765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPZ2"/>
    <dbReference type="GeneID" id="59337297"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051598">
        <property type="term" value="P:meiotic recombination checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044539">
        <property type="entry name" value="Pch2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058249">
        <property type="entry name" value="Pch2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991:SF1">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23242">
        <property type="entry name" value="AAA_lid_TRIP13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23563">
        <property type="entry name" value="TRIP13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0469">Meiosis</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="182"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="390"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="522" mass="58245" checksum="010BC5A75E801F1F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQX7</accession>
    <name>A0A8H6CQX7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155929.1">
        <property type="nucleotide sequence ID" value="XM_037298593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQX7"/>
    <dbReference type="GeneID" id="59336120"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794">
        <property type="entry name" value="THAP DOMAIN PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794:SF2">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="600" mass="64112" checksum="C4D9E5BB4EF3078E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR73</accession>
    <name>A0A8H6CR73_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">assimilatory sulfite reductase (NADPH)</fullName>
            <ecNumber evidence="4">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_007722</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155928.1">
        <property type="nucleotide sequence ID" value="XM_037298592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR73"/>
    <dbReference type="GeneID" id="59336119"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06207">
        <property type="entry name" value="CyPoR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000007">
        <property type="entry name" value="Pyruvate:ferredoxin (Flavodoxin) oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000010">
        <property type="entry name" value="Sulfite reductase [NADPH] flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000052">
        <property type="entry name" value="Sulfite reductase [NADPH] flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000011">
        <property type="entry name" value="Sulfite reductase flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF109">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] FLAVOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="19">
        <location>
            <begin position="704"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6227994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1093" mass="120981" checksum="0ACC43F0EBC02180" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRF7</accession>
    <name>A0A8H6CRF7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155900.1">
        <property type="nucleotide sequence ID" value="XM_037298564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRF7"/>
    <dbReference type="GeneID" id="59336091"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004354">
        <property type="entry name" value="Meiotic_Rec114"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03525">
        <property type="entry name" value="Meiotic_rec114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="388"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="554" mass="60950" checksum="630F29959417E8C1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRL2</accession>
    <name>A0A8H6CRL2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007949</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156153.1">
        <property type="nucleotide sequence ID" value="XM_037298817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRL2"/>
    <dbReference type="GeneID" id="59336346"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000125">
        <property type="entry name" value="Ubiquitin C-terminal hydrolase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF23">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE USP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="453"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="772"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="593"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="690"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="626"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="697"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="741"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1143" mass="128611" checksum="2BDEDB76CB3F4487" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS04</accession>
    <name>A0A8H6CS04_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CinA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156431.1">
        <property type="nucleotide sequence ID" value="XM_037299095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS04"/>
    <dbReference type="GeneID" id="59336624"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="entry name" value="CinA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="CinA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CinA C-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="173" mass="17945" checksum="7277CA09FA70C846" modified="2022-01-19" version="1">MASGFPPPAVREMAFEVSALLKERSETVCVAETAAGGLISASLLSTPGASKIYKGGLTLYTLESRIAFAGWTQDHLKSYKGPTEDVVKGLAENVRGTLSATYCVCESGTAGPTGGNTRNRTPGYVALAVATEKATSTKEAETGLGGDREGNMVAFAVEGLKLLRDVIKGNAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSJ6</accession>
    <name>A0A8H6CSJ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156370.1">
        <property type="nucleotide sequence ID" value="XM_037299034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSJ6"/>
    <dbReference type="GeneID" id="59336563"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17297" checksum="4917F20F5D201D46" modified="2022-01-19" version="1">MDPETSTFKSPIRQLTDNNYSEWLIDIKALLRGKKLWNYTQEAPDPDKSVTAQAKWRDSAMEAADLITPTISGPVKQKLQANAFDDGYLMMTSLANIYAPKGDAEFMRLTREYYSLRYEDFDSMTHYLTQIKTLEERIRDINIILDDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7G7</accession>
    <name>A0A8H6F7G7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147542.1">
        <property type="nucleotide sequence ID" value="XM_037296968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7G7"/>
    <dbReference type="GeneID" id="59334470"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="333"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="469" mass="51876" checksum="51F9189137F4F24A" modified="2022-01-19" version="1">MCQTFYFEHDDCTCIYIPANFTPCAAYSLAHAEGKSEPPNPFADPEDPPTEPDPFADPEDPFADPVPEANPLGVASRPKLLTQAYGGLDFTYDDVDFVSVDVSGARAPKGLECSEGFAPRLAHRAEGGFVVCALCDRPDVLELVGWMGMGKDEHKHKDVDMDMDMGKDKDMDLGKYNGKEEEEERKGKEKEKGKPKEKGIRHKTALVRAAVVKSFKVAVGFRAPKPGDSSADYSAIGEGESSSSTRPARPNYSIPYATPADLPDIIAIFHAAFAADPFVGQFMINVPPEVKLTYDMHCYGHEFEMSELNGLKLRKIVDGDGKTLAFAKWQYPYTLTAEQKSRKVQLDELQELEHPLPKGTNRELYHVFFAALREKKAKWMDESKDYLLHTLGVSPAHQRKGLGAMLIGEGLADADRNNARTYIEASPAGYDLYLRHGWKQVDKIVIDISKYGGHGVATEKLLLREPGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F867</accession>
    <name>A0A8H6F867_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005906</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147991.1">
        <property type="nucleotide sequence ID" value="XM_037296810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F867"/>
    <dbReference type="GeneID" id="59334311"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034760357" evidence="2">
        <location>
            <begin position="21"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="702" mass="71733" checksum="9B54F1A4ED3056AE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8D8</accession>
    <name>A0A8H6F8D8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148502.1">
        <property type="nucleotide sequence ID" value="XM_037296520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8D8"/>
    <dbReference type="GeneID" id="59334017"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF5">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE ABHD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="30"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="268" mass="28631" checksum="3462D1C9BC14D587" modified="2022-01-19" version="1">MASSINSYGSLKAHHGHIYYEQEGNKSGHSVLFIHGLGGTTNTYQPLVSSLQDFNLVRFDWAGHGRSTLPQKTSIDSYIEDAEAIINHFSLKDITVVGHSLGCLVALTLAAKQPSLVSKLVLYGPIKAPPQPGRDGAKGRAESVRKGGMAAVADTVVGNAFAAQTLKERPEIVSFGRELLSRQNAEGYALACLSLAESRDPEWGAIKAKTTIVSGSEDKVSTPALCKAIAEELGHTKVNLVTFEGVAHWHTLENAKESAEVLRKAVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8J9</accession>
    <name>A0A8H6F8J9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005965</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148049.1">
        <property type="nucleotide sequence ID" value="XM_037296868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8J9"/>
    <dbReference type="GeneID" id="59334370"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01846">
        <property type="entry name" value="fatty_acyltransferase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF85">
        <property type="entry name" value="A, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G10760)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034552489" evidence="2">
        <location>
            <begin position="21"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="369" mass="40675" checksum="CC71B7FECDDE8818" modified="2022-01-19" version="1">MSLLMSLALAFLAMASQVTGQSTVTPQPYCHPVAGNFSNNADYWLHATPVLRAHWCFDYAPLVRRQYTDYWTGWKNISFLFAFGDSYTSTSFNIAGKQPSLSNPLGNPSYPGSTSADGPNYIDFLTTTYNESYIQAYNLGYGGATIDDSIVMSGFGTSVHSFQDQVENGFLHTYVNNSKVPWTASNSLFTIFFGVNDVTNSFAPFASHNDSVNYELVKAYESLVDELYAAGARNFLFMNVPPVDRSPGTLEMGSIDQSAEASFIGAFNFRLGALVYNLALRHADTTLFTFDTNFLFTRVLDDPSQFGETAGYLNTTNYCAAYENGTSSLTSLDPSCGIPVDQYFWLNSLHPTYPMHNFMGSQIAKILSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8K0</accession>
    <name>A0A8H6F8K0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148682.1">
        <property type="nucleotide sequence ID" value="XM_037295984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8K0"/>
    <dbReference type="GeneID" id="59333478"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="225" mass="24470" checksum="F9028467B7A8563E" modified="2022-01-19" version="1">MTGFASPLASSQGLAERDNKWNLKCGNAPTVVAGSLLNASDVTNPLPQVLPDSGSNQPWLKYLWCIPGTQTYLFLNNGRLLPPPLTYRDVRGALSQAAVQAQNNLTQHGDGPITGHYSGFMTPGFNWYMTPPATNFQVYAQSSKGVLTWSVLVAALKGLGQYVNNTYDFGDVPIVFQINDGQWGEVGIGYVGFIDPDDPQEKCIYELVWGKEGYCEDVTAGKVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8N9</accession>
    <name>A0A8H6F8N9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-complex protein 1 subunit zeta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCT-zeta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005193</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148802.1">
        <property type="nucleotide sequence ID" value="XM_037296104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8N9"/>
    <dbReference type="GeneID" id="59333599"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03342">
        <property type="entry name" value="TCP1_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000058">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000004">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012722">
        <property type="entry name" value="Chap_CCT_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02347">
        <property type="entry name" value="chap_CCT_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="542" mass="59130" checksum="E98ED8C1E28F36F7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F966</accession>
    <name>A0A8H6F966_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003640</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149250.1">
        <property type="nucleotide sequence ID" value="XM_037294562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F966"/>
    <dbReference type="GeneID" id="59332051"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451">
        <property type="entry name" value="MARVEL DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451:SF4">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="6">
        <location>
            <begin position="5"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="165" mass="17880" checksum="34F64A61642D3A01" modified="2022-01-19" version="1">MFNFNLPLRVFQLILAIIALGLNGYVTSWYLHHTHPSTTPSAPPYLVFVGTSTLLTLPYLSLNPVLAGGNKPNGRFFNKWLILALDSATCLLWFAGFVALAVFHRGLILCGPCDVMVGGVIVGALAWATFLATTVLASLHIMRTRRGSGEQTHAGWVGTQESVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F976</accession>
    <name>A0A8H6F976_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148813.1">
        <property type="nucleotide sequence ID" value="XM_037296115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F976"/>
    <dbReference type="GeneID" id="59333610"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053206">
        <property type="entry name" value="Dimeric_xanthone_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38048">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38048:SF2">
        <property type="entry name" value="HEMERYTHRIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="2">
        <location>
            <begin position="53"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="228" mass="25500" checksum="966BEA37EC23EC21" modified="2022-01-19" version="1">MTLTRATRLKFMKIVAFCGDLTQVWVQRLLTGLALSAYTPSVLQKSPAAIHAASEMTHVHNVITRGVNSFYLQAPNVRNPSDIKDFLHFVIVWGNLVDRHHETEEESIFPDLENFTGDKGLMQHSVEQHHAFHSGLQTLKNYASSTAPEDYSSDKRKSIIDGLGPTLQEHLVDEIDTMLTLKKFDSEGLMKVWKVSEAEARAKTRSTGASKASPTNRKPQRKTADSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9R7</accession>
    <name>A0A8H6F9R7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_002862</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149865.1">
        <property type="nucleotide sequence ID" value="XM_037293787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9R7"/>
    <dbReference type="GeneID" id="59331274"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000095">
        <property type="entry name" value="Mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="65"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="160"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="264"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="326"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6220430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="426" mass="46773" checksum="B67C30E6F39C3287" modified="2022-01-19" version="1">MSWIVSLFNPAQSTRKSYAPSNDRQTPVFSNVQDYEDGSNSTSGKRRAGEYAQSTEEEEEEYPRHPYWQCMIAGGLGGTSGDLLMHSIDTVKTRQQGDPHKPPKYGSLSSTYVKIFRQEGFRRGLYSGVVPAFLGSFPGTIIFFGAYEYSKRHMLDAGINPSLSYLAGGFIADFAASFVYVPSEVLKTRLQLQGRYNNPFFNSGYNYRGTIDAARTIVRREGVSALFHGYKATIFRDLPFSALQFAFYEQERNWAKRWKGSKDLGVGLEIATAASAGGLAGVMTCPLDVVKTRIQTQINPPDTATPAGGATNVHPQPAKISRMEKLGSPRAAHNPSMQASSRRLISTSSPNTSMPKPDAVKLDTSSVVTGLRLIYKTEGMMGLFRGVGPRAVWTSVQSGTMLVCYQALLKRMEASPWLNDEESGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9X3</accession>
    <name>A0A8H6F9X3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome biogenesis protein NSA2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003229</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S particles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the pre-66S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eS8 family. Ribosome biogenesis protein NSA2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149533.1">
        <property type="nucleotide sequence ID" value="XM_037294152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9X3"/>
    <dbReference type="GeneID" id="59331640"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11381">
        <property type="entry name" value="NSA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.310:FF:000001">
        <property type="entry name" value="NSA2, ribosome biogenesis homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039411">
        <property type="entry name" value="NSA2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12642">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NSA2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="261" mass="29713" checksum="4979B749C1B7978B" modified="2022-01-19" version="1">MPQNDYIDRHTKLHGKRLDHDERVRKRTAREAHKVAKDSQSFTGLRAKLYQKKRHHEKIQMKKQIRQKEESNVKSAGPQEPSSTPLPQYLLDRSQPSNAKALSSAIKNKRKDSAAKFSVPLPKVKGISEEEMFKVVKTGKKTAKKGWKRMITKPTFVGPDFTRRVVKYERFIRPMGLRYKKAHVTHPELKVTVQLPIISVKKNPQNPMYTQLGVLSKGTIIECNVSELGMVTAGGKVIWGRYAQITNNCEMDGCVNAVLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAE1</accession>
    <name>A0A8H6FAE1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150662.1">
        <property type="nucleotide sequence ID" value="XM_037293009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAE1"/>
    <dbReference type="GeneID" id="59330496"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="351" mass="38463" checksum="B0A8BB968D6C2E8C" modified="2022-01-19" version="1">MPSNPPHMHTDAHDNVPRQQQFKPAAWGGASTDDPKTKTSDFKAWESMRTPRGEEGPIPFHIRTVSQKAAGPPPVPPGRETNGKATHLRNWSSSDSTDILASFCAGFGIGDDDDIFSRSLPQSTLWKERTVDDSGNRDSDGSLAPLTPSNPNDMKAPSDTSDSSSEFHSETTEETYPPPGPNPLQRKPTSGICQDGAYAMERDTIRLRRKKSCRTSERPSMGPGRSSGLFGEYGPHDKTEKAFRHSPKTSEDDKPHQHSPNDPPPHIEGVSIRSDASSPKYEGASSRHPQGSSPSDKINDLIHQFKIEYEPQCPVNVEDEVLRCKKEQLINDAQSALEQLDSIMGAANGDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAE4</accession>
    <name>A0A8H6FAE4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150570.1">
        <property type="nucleotide sequence ID" value="XM_037293741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAE4"/>
    <dbReference type="GeneID" id="59331228"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052635">
        <property type="entry name" value="Sec_Metab_Biosynth_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39607:SF1">
        <property type="entry name" value="B-ZIP TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39607">
        <property type="entry name" value="XANTHOCILLIN BIOSYNTHESIS CLUSTER TRANSCRIPTION FACTOR XANC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="51"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="294" mass="33099" checksum="4174BDBC43C5E7FB" modified="2022-01-19" version="1">MRTMQRNSSYSLPYPGPTSPKYSSSHSTSSAFSASANPNEDWTKISDLAERRRIQNRIAQRNYRKKIKRRLEDLERRAGSSSASPEQSHAELAPMIQESHTHRRNEEGVKRKPSKQDSAASKGRRRSPEPLLTPYSTPKTGRSSKSPYHYHRELSISPPPSYNYSYSLPEPVIQAPYPQHSPFNTLPAPYPDYSGQPQYLPPLPTTLPSMVPYELGPSKHNGFLDEDILSQYETGYAPFAGIDLPMQQSYQDSNAHTPPLSHSHSFEYSRDGSPSMIFPGTPVSIPDSPRLVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAE5</accession>
    <name>A0A8H6FAE5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable electron transfer flavoprotein subunit alpha, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003071</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150073.1">
        <property type="nucleotide sequence ID" value="XM_037293995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAE5"/>
    <dbReference type="GeneID" id="59331483"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000041">
        <property type="entry name" value="Electron transfer flavoprotein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="361"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="334"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="407"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="420"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="889" mass="97728" checksum="9ABDDD139BD655D3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAH8</accession>
    <name>A0A8H6FAH8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell division control protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150690.1">
        <property type="nucleotide sequence ID" value="XM_037293037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAH8"/>
    <dbReference type="GeneID" id="59330524"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012535">
        <property type="entry name" value="Cell_div_Cdc14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34065">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34065:SF1">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08045">
        <property type="entry name" value="CDC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="42691" checksum="A1862820659231AD" modified="2022-01-19" version="1">MKRRSKRTGQTRLVRLAGGSLMSRQRHVYKLPKADQTAQERFSHHLTAKSIKPKMESLLSLSFDYLSSYDGVKIRKGLRQLEGLLAQICLSRTPTDRRSSVISGGASKQTTKELSSLKDDPAFREFFKLQEGFEWNVAIRLIACLERLLSKASNGQNDLLIVSTLELLQGTLLIHPPSRSLFAREIYMNLLLDLLDPTCCPAIQSSTLLALVTTLLATPANTRAFETLDGLLTVTSLFKSRGTSREVKMKVVEFLYFYLMPETPVSNGAGGGLHRSPSKRKGSGEREGRQAGTKTTEEKQGLLGRHLSNVEDLVEDLREKMFLLRSADEGGEHVFQFYEISQLPLQLQDDPVKQFQSDIEQQLVHSNKQGREQERARRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBW8</accession>
    <name>A0A8H6FBW8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000790</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152088.1">
        <property type="nucleotide sequence ID" value="XM_037291726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBW8"/>
    <dbReference type="GeneID" id="59329208"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12168">
        <property type="entry name" value="Mand_dh_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000203">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase (SerA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF257">
        <property type="entry name" value="GLYOXYLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034233683" evidence="5">
        <location>
            <begin position="23"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="6">
        <location>
            <begin position="36"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="7">
        <location>
            <begin position="126"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="365" mass="39738" checksum="821BE36A31EF7036" modified="2022-01-19" version="1">MLLWEGKKPIVLLLGEVILAQAEWNDLGALAELRQVNEGTRDRFLIDCKAGVYDGVVAISRTYDSTQMTGRFDQELIDVLPSSLRFVSHNGAGYDSIDASACATRGISVSNTPGAVDASTADTTLFLIIGALRRIHIPTTALRAGKWRGRMCLTHEPEGKTLGILGMGGIGTAVARRAVPFGMRIQYHNRSRVPASQNPVGAKYVSFEELLRTSDIISVHLPLNASTRGMISRDQFATMKDGVVLINTARGPIVNEDALVEALESGKVWGAGLDVHEHEPKIHPELIENENCVLLPHVGTGTLETQRKMEVLVCENIKSALEKGHLKTPVLETRKMPTAKGVRYNDKMGLRNGVGRYDGHLQGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC42</accession>
    <name>A0A8H6FC42_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151843.1">
        <property type="nucleotide sequence ID" value="XM_037292424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC42"/>
    <dbReference type="GeneID" id="59329910"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="276" mass="32022" checksum="86B53C3961A299FA" modified="2022-01-19" version="1">MSHTDREQVIDQDGDRMSHTDRENSHSCAGKGTFSFLQLPAEVRNMIYHHCLTSETIVPRYSAWTKRWTPIDLLYVNRTIYNEAFFHLYTKGEFVLVIRPESLFALASCWATNDISASVGLELFFKTAKILDLIRHIRLKIHWPSVQYSTLMDRKISRRAPTTDNMLKRTIRTTGAMLSELPALRTIDVSWYHMTVCPWELAKVAPPDYKIPVWLRGLKQVRRENEKVLIRMPSTGPVSTEELAQGQGDRDELANLLKEVREDLQELKGCLQERLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC87</accession>
    <name>A0A8H6FC87_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_000850</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152147.1">
        <property type="nucleotide sequence ID" value="XM_037291785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC87"/>
    <dbReference type="GeneID" id="59329268"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15844">
        <property type="entry name" value="SNARE_syntaxin5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000007">
        <property type="entry name" value="ER-golgi SNARE complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021538">
        <property type="entry name" value="Syntaxin-5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF3">
        <property type="entry name" value="SYNTAXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11416">
        <property type="entry name" value="Syntaxin-5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="259"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="23"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="186"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="351" mass="38781" checksum="F42F79F141B674AD" modified="2022-01-19" version="1">MTTTSIQDRTAEFRAVLAQAQKRQSSTKIGAQRQSLLTESQRRAANGSPNGDIKGARRGARSEFARRAAEIGRGITGTMEKLGRLAELAKRKTLFDDRPVEINELTYVIKQDLSSLNSQISSLQSLSKSQQTQTSRNGAEQEGEHNKNVVVLLQGKLAGVGVNFKEVLEVRTKNLQASRSRTENFVSSVSAHSQPPLNPQQSASPLYNTPTKSRTPQPGYQNSNHDILNLDPGSSSALMQTGAQSDQQLLMMEEAQPQNTYINMRGEAIDMIERTINELGGVFGQLAAMVSEQGEQIQRIDANTEDVVDNVQGAQRELMKYWSRVSGNRWLVAKMFGILMIFFLLWVLIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCJ8</accession>
    <name>A0A8H6FCJ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000969</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152264.1">
        <property type="nucleotide sequence ID" value="XM_037291902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCJ8"/>
    <dbReference type="GeneID" id="59329387"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="213"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="413"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="600"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="619"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="637" mass="69739" checksum="ACDDB4E7D3EE8743" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD30</accession>
    <name>A0A8H6FD30_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000469</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152843.1">
        <property type="nucleotide sequence ID" value="XM_037291408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD30"/>
    <dbReference type="GeneID" id="59328888"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000005">
        <property type="entry name" value="Similar to RNA processing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF3">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="190"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="419" mass="48191" checksum="56470B092FAB8BF4" modified="2022-01-19" version="1">MASQKLKLSAKAHLPFKSKNKEKRQSLHINRKRATDSIKRDERFRRRREEDKNPTLREQRLKENVPLTLERKRVWDDVDSDVGDGLGVSVNIERLKRRKVEEEQAALEEGEEEEGEERSEGEDEERDSMIESASEDEAIEIVKHAKAPKRRTPTERATSPVQSMRSTNLDFTPEALAAKFPTLFSTDAPPVPKVLITTSLNSTLHKEADHLTSLFPHSVYIPRSSHRYSHKFSVREICSFATNRKYTAVIVLKEDLKRPTGLTVVHLPHGPTFHFTISNWIEGKRLPGHGNATDHTPELILNGFSTPLGLLVAHLMRTLFPAQPELQGRQVVTLHNQRDYIFVRRHRYVFRDKRKTEKSVVDADGKEVKGAEEIRAGLQELGPRFTLKLRRVDKGIQRASGQEWEWQGGMEKQRTKFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDY4</accession>
    <name>A0A8H6FDY4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA topoisomerase 2</fullName>
            <ecNumber evidence="8">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">DNA topoisomerase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">HO133_010905</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 21">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the type II topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153388.1">
        <property type="nucleotide sequence ID" value="XM_037301754.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59339295"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16930">
        <property type="entry name" value="HATPase_TopII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03365">
        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.268.10:FF:000003">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000003">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000004">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034157">
        <property type="entry name" value="TOPRIM_TopoII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="17 21" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="23" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 21" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="378"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="424"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="24">
        <location>
            <begin position="1141"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="25">
        <location>
            <begin position="1393"/>
            <end position="1840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="579"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1872"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1982"/>
            <end position="2021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="2054"/>
            <end position="2268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="1982"/>
            <end position="1993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="1998"/>
            <end position="2007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="2129"/>
            <end position="2153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="2173"/>
            <end position="2182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="2200"/>
            <end position="2221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="2222"/>
            <end position="2236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="2244"/>
            <end position="2256"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="21">
        <location>
            <position position="1483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00019635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00053943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAF6224328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2268" mass="252377" checksum="B55CC8D8D4D0A2B1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE49</accession>
    <name>A0A8H6FE49_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153618.1">
        <property type="nucleotide sequence ID" value="XM_037300801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE49"/>
    <dbReference type="GeneID" id="59338337"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="505" mass="56701" checksum="B3D500AFE7332C0D" modified="2022-01-19" version="1">MELQVATLGFLPNLNSPTAPSTFSQVNGGGVFITAAPQGCFFDTLYHAVSFASLSCFDRVLEKDVFLTFGQIHEDITEAVFGLGPPTRVLLMQLQKRKGTLIAKRSFLHQIRRIAFSGIGELLAHLDSSKGLRISDHSCIRYGKQEIQPSHHDDIIELHNAPHKKPKVDGARALANELTNLAELIIYSWSFPGSHYSFWFVDRSSPSDVDPSKASIALGEDLVFKTLMVIMSRIFERQLSLRYKAVETAVRLRRTCDHFNTALTAIQRVENRLGTKKKPDGEAVELDKAKTLRKILGVLMGIYDVMRFTFNRSAFVHRHLPPSNRRRHCKISICEQSGYRIPCEGRSNYVSTFRSKSGIVLVIVAAKIFTSRLAELTKLIDFSIRKIMQLRVFYAHSVNRCKNVNAEPLFMENRHLLKCIKDGSAKPGQERFLVLTTPVCYDKYVQGILKSGWVHNFSAGQIYVDEFHETKSDDAPSYGGAISADEPGFGKVCILVSFQVPDSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEC9</accession>
    <name>A0A8H6FEC9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CobW-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009973</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153646.1">
        <property type="nucleotide sequence ID" value="XM_037300829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEC9"/>
    <dbReference type="GeneID" id="59338365"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF31">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="8">
        <location>
            <begin position="39"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="CobW C-terminal" evidence="9">
        <location>
            <begin position="317"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="42254" checksum="5DA0A77029B34ACB" modified="2022-01-19" version="1">MFVDEEEPPNLVDVTHPSASAVANPITSRLQDISLAKVPLTIVTGYLGAGKTTLVNYILKEQHGKKIAFGDSADVEKALTVTQDGQETQEWLELANGCICCSIKDSGVNAIESLMARRGTFDYILLETSGLADPGNLAPLFWVDDGLGSTIYLDGIVTLVDAKNIVLSLDEMPVEGKGDAEHEGMHMTTAHLQISHADVIVINKCDSVTPEQLGTVEKRIAGINGLAKIHRTQYSQVPKLESFLLDLHAYDGVESLDAMSKGHSHLDPTISTIALPLPPLTPRQLSSLESWLRSVLWESILPVVPIPGRDSRSESAFAIHRLKGRIFTTTDAVKMIQGVREVFEIVDLDRGRSDGKGMESKEEGKLVLIGRGLDEDAFGRSLRARLEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEP4</accession>
    <name>A0A8H6FEP4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154329.1">
        <property type="nucleotide sequence ID" value="XM_037300481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEP4"/>
    <dbReference type="GeneID" id="59338015"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="266" mass="29991" checksum="999ED2B714ADC802" modified="2022-01-19" version="1">MDKGRFAVYRTVDVNVVDIVDRLGKGITGKEKHHDMWHAIPESFIGSTPTQLIHHHRSRSKSLNQSLLVIADRQDLAAEGLLVVNLDFKGSIDALRQKIFFAGDFVPSVNIGNTPWEETLSNATLPLYPRRKFAVFADRGFHPRYLMTEALKRMNAGLGNRRAETGSIFADWESAPGDDVGEIVRFWRENARERDWDARYFVLVKKDAWDQGKADMISLQDGQDKAHETTINVDRIGEVLVQLYVGLITLDEVKETNMDNQGGAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF75</accession>
    <name>A0A8H6FF75_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009909</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154615.1">
        <property type="nucleotide sequence ID" value="XM_037300767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF75"/>
    <dbReference type="GeneID" id="59338303"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="398" mass="43835" checksum="D605A2F4B44A905B" modified="2022-01-19" version="1">MLQHRIHDIGDRYRDAFRRFFTAEPISLHGHVSDASSPALLTSLISHLASRGSTNGSSPFLEEADKANPCVAILEGSYGAGYGPLAHNSVLEFVRPLANGRNPIVIKTHNAANIDDQVRKAKRTGCVALIAEIVRARDGVVISQSAWRHLLRACEKNNLVLVVDEALTAIRCGAPFAYQLPQFQKHGVPDLVLFGKAVRTNGIAVDWRGVNMKKLEVTDPEERLFIALQWQERLTEMAPAASLLTSWGTIVLAEKEQWPQRAREIGRILRNLIESEGIKSSLIGGSNSLIYLRVQDQARITSPVMGANAGKYVRWFPMMDAVMTSERELSTKVFGPGSIPHRRDVSAYLTSQAGGTLMSDGRHEQMKYAKDGSIVQLLPKHQGYAAELVLANSEAIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFF5</accession>
    <name>A0A8H6FFF5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154597.1">
        <property type="nucleotide sequence ID" value="XM_037300749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFF5"/>
    <dbReference type="GeneID" id="59338285"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="451" mass="51953" checksum="602D5102D6D5FEAF" modified="2022-01-19" version="1">MEIIRMPVELLIEIFMKCDIKKDWKVLRLAPNMLRSLHVCMPNYPYDRVGKEARVAKSKLLWALRSCPGLHSLTTYDPNVYRNNRGLGASEPWHDLKPEFLLPQLAELTIADRTFEVDDLLKWGAKGGWTKLIKITLWDDRLLYGFQGCEQSLRSISLLHTKAGNENALVSICSRTIRLTELKIKTEESRLPFSALMICGPSLETLAIHPHRDALDNWINMQDDHLSALSQIPQLAEITLHTQKPQGYEKHKSSLAAVEHAFRHVIEHGGNEQNLRSLILYDGPQTSDWDNPWGLVERSSRAFKATRGTHGGNMDQNICAKHIQGPSETDHERYHRQQERVEAEKKTRDYLDAMPPAVLSQMIDNNRRQIRGTTSDIQEQMYEIQTGGWNPELAEYEERWKNSSGILPMLEWQLQTRKKQGWKLWDMPLMRSDAGSEGFLYDRMMEASAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFR5</accession>
    <name>A0A8H6FFR5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154772.1">
        <property type="nucleotide sequence ID" value="XM_037299946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFR5"/>
    <dbReference type="GeneID" id="59337480"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034890049" description="Thaumatin-like protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="25596" checksum="72FE1D5E646305E0" modified="2022-01-19" version="1">MHLTNTLVPFLLTLASPALAAEGVISKDGLGTAIGGALDPTVTPAPKPAVGAAPAVAGPLTIAVTNSFGAGLSISFQHDSGSSSAINNPTPGPLGGATTIIYPSGWAGRIAVGKVMSVINSGSKIEGSFSLPTYQPNIDVSYVDGYTIPITCSCGGSVVTGCNHPLFADPITCANEGPGPICYNPLCASDQAEGTAAAPFFEPCRGAAYTYPNDSGCNVDYVSCCVGSTCPAPAKQHGKRSLVGGHRQREAVDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG01</accession>
    <name>A0A8H6FG01_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008578</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155445.1">
        <property type="nucleotide sequence ID" value="XM_037299444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG01"/>
    <dbReference type="GeneID" id="59336974"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="224" mass="25198" checksum="65501C0C77E016C1" modified="2022-01-19" version="1">MPAEILNRLEASIPEIEASFVNENIGSSMKADSLDPLESELRAMKFIWKLPIVSIDFLHEDEKFCGMCDRKYDDEFRVCGQCLRKYLSPYECGFTKCPFCKVDFPQTFTDPVEPTLATSDLAWVDVDDHEVLDEGLSRQVSQLSKDSGAPSDEIKRYLSIDEVLERSRSQTEDYMGTDVQDFATQATDPAEIGDEEFPKNRKNGGGPSLARAAMKAVDLIAKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGC4</accession>
    <name>A0A8H6FGC4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155172.1">
        <property type="nucleotide sequence ID" value="XM_037299171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGC4"/>
    <dbReference type="GeneID" id="59336701"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="137" mass="14834" checksum="B8003458808A22FD" modified="2022-01-19" version="1">MSAISNATPEQLQALLNGPALKPPPGVIPDFTDPSTSHRRGVAVEIVSLLLSAFAVAMHIYTKARFMHQMAAAACTTLLRNPLGMRVSSVLCEVCFNTSHGFLVGYFIIGWLVSDIAPGAKQWDVQLENLGSFLYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGZ9</accession>
    <name>A0A8H6FGZ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH:ubiquinone oxidoreductase 30kDa subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_007658</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155864.1">
        <property type="nucleotide sequence ID" value="XM_037298528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGZ9"/>
    <dbReference type="GeneID" id="59336055"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.80:FF:000002">
        <property type="entry name" value="NADH dehydrogenase iron-sulfur protein 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase, 30kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01357">
        <property type="entry name" value="NDH1_NuoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010218">
        <property type="entry name" value="NADH_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020396">
        <property type="entry name" value="NADH_UbQ_OxRdtase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01961">
        <property type="entry name" value="NuoC_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004733">
        <property type="entry name" value="PRK06074.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="Nqo5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00542">
        <property type="entry name" value="COMPLEX1_30K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase 30kDa subunit" evidence="10">
        <location>
            <begin position="106"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="252"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6227930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="283" mass="32460" checksum="CFF189AE2A139C7D" modified="2022-01-19" version="1">MAVRLSAATSVKNVARIFAKQPPQLSKTRQFTCTAIRAKEVANQDELPNLRHAQRPPGGKLHAPVVNPTDKFQEKAASLHTYGQYLLSCMPKYIQQFSVWKDELTIYIPPTGVIPVISFLKYHTAAEYTMVADITAVDYPTRSNRFEVVYNLLSVRHNSRIRVKTYADEATPVPSITSLYDGANWYEREVYDLFGVFFVGHPDLRRIMTDYGFDGHPLRKDFPLTGYTEIRYDEEKKRIVVEPLELTQAFRNFEGGTAGKDRKPEEFQLPTPKPEEKKEEPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIA6</accession>
    <name>A0A8H6FIA6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156696.1">
        <property type="nucleotide sequence ID" value="XM_037298061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIA6"/>
    <dbReference type="GeneID" id="59335568"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="92" mass="10498" checksum="A5E26FB7F3165E1C" modified="2022-01-19" version="1">MHINYFRTYLKTASGRIKDMVEIRQKYQGKGAFEQADLKGYVATQLDFSDMGFSVHAAHLDSAEPVCAEKDIAPWLFVSLFGQLLECYIDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIF2</accession>
    <name>A0A8H6FIF2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA damage-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007232</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156760.1">
        <property type="nucleotide sequence ID" value="XM_037298125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIF2"/>
    <dbReference type="GeneID" id="59335632"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000629">
        <property type="entry name" value="UV-damaged DNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="6">
        <location>
            <begin position="12"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="7">
        <location>
            <begin position="416"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="5">
        <location>
            <begin position="773"/>
            <end position="1091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6229118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1128" mass="123302" checksum="6ED79FA98AAFE53C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIQ2</accession>
    <name>A0A8H6FIQ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156839.1">
        <property type="nucleotide sequence ID" value="XM_037298204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIQ2"/>
    <dbReference type="GeneID" id="59335712"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="132" mass="14775" checksum="2ACC92FB1830A04A" modified="2022-01-19" version="1">MENVEAVASSLVNADYHGHSTTKIRESQENDAITGNERQVERFVQDEVDISEFHLAGTAYGQDEAALDDDIEREEEEDESEEEEGEYESGEEAVSTVKDNLSIRTIIDCTISVQGLLRRAFVSGDGENHQMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIR1</accession>
    <name>A0A8H6FIR1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4385 domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156956.1">
        <property type="nucleotide sequence ID" value="XM_037298321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIR1"/>
    <dbReference type="GeneID" id="59335829"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025494">
        <property type="entry name" value="DUF4385"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14328">
        <property type="entry name" value="DUF4385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20511" checksum="7959B513C31B562E" modified="2022-01-19" version="1">MSTSPSASISCQPPWARYRIGRGETGVLTFEPYKSALLPLWRFRTPGLARKSSSDLWTAFLDYENEDDFVGMDMARKFLQMGMTRARRYANHKEGRKYCPAGEGSGEVLNGNGDGWKGSEAWKEKDEASRVFKEVWDRARALKGYQAKKAEFLKKQKEWDKQRKVESGKEGRKRRSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKG4</accession>
    <name>A0A8H6FKG4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004535</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157453.1">
        <property type="nucleotide sequence ID" value="XM_037295452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKG4"/>
    <dbReference type="GeneID" id="59332943"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000273">
        <property type="entry name" value="Leucine Rich Repeat domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF69">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 11-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="286"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="598"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="609"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="641"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="783" mass="86098" checksum="782E36914C68EE64" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKP0</accession>
    <name>A0A8H6FKP0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase family 92 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004639</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157556.1">
        <property type="nucleotide sequence ID" value="XM_037295555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKP0"/>
    <dbReference type="GeneID" id="59333046"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF69">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13704">
        <property type="entry name" value="Glyco_tranf_2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6230299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="38558" checksum="A3AA8B1DC0C84568" modified="2022-01-19" version="1">MAHSQSLSNDSDDERPIDKPLLTSTKPPANIPFKEAPVSSPDSEPGLEDAGPIPNNLTVAFPPLPPADDEEYVSLCLAIKDQPGDLDEFLAHYYYHHGIRRFYVMDDGSDPPLSYTPNFGIPPFAVPRSAVTFTYIPRVPDRPQPFQHTIYADYCIKNFGERHTWMGFFDADEFLEMRRATTLVDWLHQWEKNETVGALSVNWITHNSGGQQKRPQSDTRKGFRRCVVDGPDGRNADKENSHVKSFAKTALFDGVNSVHNMRTAYGTVSVGETGDECMIKRFPITHDMWALHHYSVKSREQFLEKQQRGSPNNHHAAGEFWDRIEDADDYECPELASYVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKQ2</accession>
    <name>A0A8H6FKQ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-phosphogluconolactonase</fullName>
            <ecNumber evidence="4">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004655</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157572.1">
        <property type="nucleotide sequence ID" value="XM_037295571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKQ2"/>
    <dbReference type="GeneID" id="59333062"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000005">
        <property type="entry name" value="6-phosphogluconolactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="104"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6230315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="351" mass="39131" checksum="B36C07CBB17846C3" modified="2022-01-19" version="1">MISRIDSSSLDPQIPFCSKCVEELKRKMTADFHEERREMTRVARVRDWTDEEISAMRIELRDQLYEELKSVNKPLPPLPGGDDSASRRRTMGKQPNLYSFDGIDSLATALRKYVLQCQSAGFERHNAFKVAVSGGSLPKTLTKALLAQDDEAGKVDFSKWEVFFADERAVPLDHEDSNYKVLKDELLDKIPESMGKPIVHPIDVHYMGDVQEMADQYQDALMKSFAAKDSVKIPIFDLLLLGCGPDGHTCSLFPNHELLRESDAWVAPIEDSPKPPKKRITLTLPVVTGGIKIAFVATGEGKREILSKILDTEEGRSLPCGLVNEGGAEKVSWFTDHGAVQGVSFPRRGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FLJ1</accession>
    <name>A0A8H6FLJ1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157839.1">
        <property type="nucleotide sequence ID" value="XM_037290965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FLJ1"/>
    <dbReference type="GeneID" id="59328444"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="103" mass="10463" checksum="7493EE499A0E3200" modified="2022-01-19" version="1">MAAPIMATYHGCLYHGYLPWLPLPWLPLSMPATTMPTIEIHGSSSYGSGSYGSGSYGSGSHNRGGHGRGGHGRGSHGRGSHGSGSHGRGSHGSGSHDSGSHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYV5</accession>
    <name>A0A8H6KYV5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-type proton ATPase subunit D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">V-type proton ATPase subunit d</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 11">Vacuolar proton pump subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_007494</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157020.1">
        <property type="nucleotide sequence ID" value="XM_037298385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYV5"/>
    <dbReference type="GeneID" id="59335893"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3240:FF:000002">
        <property type="entry name" value="Vacuolar atp synthase subunit d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="208"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6229378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="255" mass="28712" checksum="ACB95B1832B94056" modified="2022-01-19" version="1">MSGAGDREAVFPTRQSLGAMKNKLKGAETGHSLLKRKSEALTKYRAEITRRIDEAKRKMGRVMQIAAFSLAEVTYAVGGDIGYQIQESAKQARFRVRTKQENVSGVFLPQFESYTTEGNNDFGLTGLGKGGQQVQRCRETYTRAVETLVELASLQTAFVTLDEVIKVVNRRVNAIEHVIIPRTENTIKYINSELDELDREEFYRLKKVKSNKQRDNNAAEQEEKASKRRGSDKENADDPGATDILGEQEDQDVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZG4</accession>
    <name>A0A8H6KZG4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157133.1">
        <property type="nucleotide sequence ID" value="XM_037295132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZG4"/>
    <dbReference type="GeneID" id="59332622"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034316793" evidence="1">
        <location>
            <begin position="24"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="28800" checksum="5BF4FF9FB2124827" modified="2022-01-19" version="1">MHITHISLKALASVAVFARHAAASANLSNSSTERLSPIVVDSIPAPDTQPEFKFDFNVIFEGGKCSASQQAAILLTMKNIAGFSKRARLWKEDEFHDWSGEVTHWFGESAHTQAAWIRNNFLRVSTAINLRKIEYTNTWLWVGCDYGWTIPNLACNPYNHAFNFLNKVAWFNRYDRIFWNICPGFWSLPTAPDKIQSTIDDPRKNIHYMENYLNTREQWLFSGIISIRSIGIKESNVGVHQTYHNFWRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6D4</accession>
    <name>A0A8H6C6D4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146901.1">
        <property type="nucleotide sequence ID" value="XM_037297700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6D4"/>
    <dbReference type="GeneID" id="59335204"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11069">
        <property type="entry name" value="CYP_FUM15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000051">
        <property type="entry name" value="Cytochrome P450 monooxygenase (Fum15)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF227">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="488"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="60368" checksum="260CAD1312D75A0C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6V3</accession>
    <name>A0A8H6C6V3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_006469</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147492.1">
        <property type="nucleotide sequence ID" value="XM_037297367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6V3"/>
    <dbReference type="GeneID" id="59334870"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17947">
        <property type="entry name" value="DEADc_DDX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="314"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="521"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="156"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="199"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="700"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="664"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="700"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="752"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="787"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6218057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="815" mass="89976" checksum="A369076B739AD7E7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7K9</accession>
    <name>A0A8H6C7K9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">U3 small nucleolar RNA-associated protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_006508</name>
        <name evidence="13" type="ORF">HO133_006658</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Mckenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in nucleolar processing of pre-18S ribosomal RNA. Involved in ribosome biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
            <location evidence="1 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the HEATR1/UTP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147531.1">
        <property type="nucleotide sequence ID" value="XM_037297406.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59334909"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012954">
        <property type="entry name" value="BP28_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022125">
        <property type="entry name" value="U3snoRNP10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040191">
        <property type="entry name" value="UTP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457">
        <property type="entry name" value="BAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08146">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12397">
        <property type="entry name" value="U3snoRNP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01036">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 11" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6 11" id="KW-0698">rRNA processing</keyword>
    <feature type="repeat" description="HEAT" evidence="10">
        <location>
            <begin position="588"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="BP28 C-terminal" evidence="12">
        <location>
            <begin position="1512"/>
            <end position="1665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6217556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6218096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1795" mass="198794" checksum="16B92725A943BD74" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C894</accession>
    <name>A0A8H6C894_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PKS/mFAS DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148130.1">
        <property type="nucleotide sequence ID" value="XM_037296949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C894"/>
    <dbReference type="GeneID" id="59334451"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF29">
        <property type="entry name" value="ASPERFURANONE POLYKETIDE SYNTHASE AFOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS/mFAS DH" evidence="2">
        <location>
            <begin position="98"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="190" mass="21666" checksum="B679AFDFB9551D08" modified="2022-01-19" version="1">MQGPTKDILRGIDKDGISYDSVMVRSVSALDTLLDAVDRLTCFGYPVDISEVNKSGMKPAIQMVLPNLPEYPFDHSRSYWHDSRYNKDGSRFRNNLRLDLLGTPVSDWNPLGARWRKIIRISETPWIEDHKVWESPRMLVMALEACKQPAKEKRRVAGYTIKDATFHNPLPIAPGPNGVEVQLCLRTEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8D9</accession>
    <name>A0A8H6C8D9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148300.1">
        <property type="nucleotide sequence ID" value="XM_037296318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8D9"/>
    <dbReference type="GeneID" id="59333814"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040632">
        <property type="entry name" value="Sulfotransfer_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17784">
        <property type="entry name" value="Sulfotransfer_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15475" checksum="698FFC12ADF63CD8" modified="2022-01-19" version="1">MSLPTDLAYRKRDIVSLKSTHHPLSRSKSSELAAMANNPSLSEKRAMTKEERFPELFVNTNIDRRICRRVVPLKVIVVGMPRTGTQSIRVALKQPGFNDTYHMDCLFENPPDIDLWRQAFLAKFKSQGEPFGKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8G0</accession>
    <name>A0A8H6C8G0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005438</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paxB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148330.1">
        <property type="nucleotide sequence ID" value="XM_037296348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8G0"/>
    <dbReference type="GeneID" id="59333844"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039020">
        <property type="entry name" value="PaxB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42038">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42038:SF2">
        <property type="entry name" value="TERPENE CYCLASE AUSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25129">
        <property type="entry name" value="Pyr4-TMTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="27257" checksum="C186F07BF933E20E" modified="2022-01-19" version="1">MPFHLPLSPLDRRNQPPVYYLQVQDALILISSFLWTIAYALYVRQAFRDKSYGMPLLCLWANIAWEFLFGVVAPTSAAQTIAFVPWLLIDVAIVYTTWRFGPDQWKRSPLVANNMGWIVTSGTILMTGLFWTFIKTVGVEAASFYIGYGDQLLISITSVAQLMSRNNTSGHSLGIWFYRASGTCLTILLFAWRYWHYADSYPRVAQPTTLFLFVGSEVVDSIYPMVYVFLEKKAKKKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8I8</accession>
    <name>A0A8H6C8I8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PQ loop repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005373</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148265.1">
        <property type="nucleotide sequence ID" value="XM_037296283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8I8"/>
    <dbReference type="GeneID" id="59333779"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF37">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="30169" checksum="7DA2C11F7DD2F0DE" modified="2022-01-19" version="1">MDVPIAANILGTIGAICWSVQLIPQIIINYRRHHTTGLQPSMMLLWASAGVPLGVYNITESFNIALRIQAQILTFLSLLTWSQCYYYGKHWSLIKCAVILVGMCTLMGGIEVALIFALKAAIKRHVTWPTTLMAVLAAVLLAAGVLRHYWDIYTHRTVRGISFIFVGIDAAGDLFSLVSVFFQTELNVLGMAIYGTELALWTGVFICGGYFNLLPWVKARVSTRRQRIENERQTQSQQGERGTIALHEMPSSTSVFMTPSSGLVERTATGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8S1</accession>
    <name>A0A8H6C8S1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005386</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148278.1">
        <property type="nucleotide sequence ID" value="XM_037296296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8S1"/>
    <dbReference type="GeneID" id="59333792"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05370">
        <property type="entry name" value="SDR_c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196:SF1">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="259" mass="28752" checksum="2AFD2D372205AD2F" modified="2022-01-19" version="1">MAPQNLEFKCAIVTGGGNGLGRAISEWLISQGKKVIIVGRTESKLQTAAKEMGHSTPYYTLDTGNISAIPSFIKQVVKDHPEVDCLINNAGVQRPLEVNNFDLQKADQEIDINIRGPMHLAIGLLDHFKSKPNGALIVNVSSVLGYIPTSIVNPVYNGTKAWVHFWSMNLRTQLKDTKVRVVEIVPPTVATDLHRERSDPDDNKKEKNPSALSVDEFMDFITKDWKADKETIGAGPSQKVVDRWYHEFGPDYEKVAGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9D4</accession>
    <name>A0A8H6C9D4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex I-B22</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase B22 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_005135</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Mammalian complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I LYR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148745.1">
        <property type="nucleotide sequence ID" value="XM_037296047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9D4"/>
    <dbReference type="GeneID" id="59333541"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20263">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045292">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033034">
        <property type="entry name" value="NDUFB9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B22 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="16">
        <location>
            <begin position="5"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6219310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="116" mass="13608" checksum="34632942B5E6AC26" modified="2022-01-19" version="1">MTSKQAVTSLYRRSLKLALDWAVHRYIWRGQAQYIRSLFDANRDVREPRQQRVLFQETEALLEKWKHPDPYRPPTAPGGSKYERNIPPPILDRMYMKSTALRIAGSHHVAPPHMTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9V3</accession>
    <name>A0A8H6C9V3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mannitol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003978</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148944.1">
        <property type="nucleotide sequence ID" value="XM_037294897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9V3"/>
    <dbReference type="GeneID" id="59332387"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050085">
        <property type="term" value="F:mannitol 2-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019594">
        <property type="term" value="P:mannitol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05352">
        <property type="entry name" value="MDH-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000090">
        <property type="entry name" value="NADP-dependent mannitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF3">
        <property type="entry name" value="MANNITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="28396" checksum="DACCA6F32B4CD4A4" modified="2022-01-19" version="1">MPIEPPKSDSLKELFSLKGKVVVVTGASGPTGIGTEAARGCAEMGADVAITYSSRAEGGIKNAKELEEKYGAKAKAYKCNVGSYADVEKLISTVIADFGRIDVFIANAGRTADAGILDSSVEAWNEVIATDLTGTFNCARAVGLHFRERKTGSLVITASMSGHIANYPQEQTSYNVAKAGCIHLTKSLANEWRDFARVNCISPGYIDTGLSDFVDQKVQQMWHDMIPLGRDAKAKELKGAYVYLASDASSYTTGADIVIDGGYTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAU2</accession>
    <name>A0A8H6CAU2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003601</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149211.1">
        <property type="nucleotide sequence ID" value="XM_037294523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAU2"/>
    <dbReference type="GeneID" id="59332012"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106335">
        <property type="term" value="F:tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000219">
        <property type="entry name" value="tRNA (Carboxymethyluridine(34)-5-O)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="3">
        <location>
            <begin position="54"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="231" mass="26011" checksum="75D77DACC7B3E6E9" modified="2022-01-19" version="1">MTSNVPKGNIRQYEEIHVHQVYEEIASHFSSTRYKPWPIVERFLKDLAPGSVGLDIGCGNGKYLAVNPNVFIVASDRSVNLVKIASKHQPHSYLLADNLTLPHPLGLFDFAISIAVVHHLSTPQRRIQAVAAVLELLRRSTGAEIGGRALIYVWALEQKHSRRGWDEGDDQDVLVPWVMKQGPEEGKTFNRYYHLYRQGELEHDIVEAGGVVLESGYEKDNWWAIAAPKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAX0</accession>
    <name>A0A8H6CAX0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149510.1">
        <property type="nucleotide sequence ID" value="XM_037294129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAX0"/>
    <dbReference type="GeneID" id="59331617"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="29560" checksum="92A130DD29ECD783" modified="2022-01-19" version="1">MAVHETQVQPERDQPARTPLPVYSMLPSALQNSLQPLVLVRRSLTDYGLRSRRRVTSLDMYRGESEHTQVLPDKMAVAGECTADSAEVPGISWKFARHESQTSGAQSQAFSRQLYLDAMAYLIQGLPSDLSDQETLHLKNALPDSLKDSTSPETTQSRHRNPSLLHRSLASTIIGICLLLRLALPYIKLFIAAASSYDRTHHVRERIFAFSVTAADLFGRRSMALASTAMTNEVVWGAVKYWVDGIRGGLNEGLGEGLKVIEAQNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB57</accession>
    <name>A0A8H6CB57_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoethanolamine N-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.103</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003846</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine = phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25325"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25326"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-methylethanolamine phosphate + S-adenosyl-L-methionine = N,N-dimethylethanolamine phosphate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25321"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57781"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:25322"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.103" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149456.1">
        <property type="nucleotide sequence ID" value="XM_037294768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB57"/>
    <dbReference type="GeneID" id="59332257"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000234">
        <property type="term" value="F:phosphoethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307:SF2">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="240" mass="26305" checksum="F53FFCDB7B5D1B0B" modified="2022-01-19" version="1">MENTQPSEPTNSDEQFWTDLGLKYEAAFGHDIGLHKVIQRYLKMLPASGHILDCGPGTGKPVAAAIAESGRRVHGIDMSPGMVSLSRKAVPSGTFEVANMLEWAPTVSYDGAVASLSIFELSRQEIATMSHAWFSWLKPGGLLLINTFGAEDCQQVKAGNYDADGQCANKVEWRFMSKKILITLLTKAGWKVLLEKAGFEIVHTEEDLFTPPAAADCDPEPRYYIIAEKPSSTSRFHGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBZ5</accession>
    <name>A0A8H6CBZ5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_003115</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150117.1">
        <property type="nucleotide sequence ID" value="XM_037294039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBZ5"/>
    <dbReference type="GeneID" id="59331527"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000015">
        <property type="entry name" value="lymphoid-specific helicase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="127"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="534"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="401"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="420"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6220682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="771" mass="87823" checksum="C7FA162858FD88B9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCE6</accession>
    <name>A0A8H6CCE6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150430.1">
        <property type="nucleotide sequence ID" value="XM_037293601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCE6"/>
    <dbReference type="GeneID" id="59331088"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF4">
        <property type="entry name" value="ESTERASE ALNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="2">
        <location>
            <begin position="80"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="319" mass="35667" checksum="AF9CEA430409A26A" modified="2022-01-19" version="1">MNRPFECRSDTVPIVQDLEQDGTASFHFIHGDIESPPPNEFKEFYGSPPHRRFLKQYCSGRADYEFFNALWSAPHRLTPEQKYREMVQSLRPESTNDVQTILDRLYRVLDHDGPFDGIIGNSEGASVAAIFVVNYLTRCARKEIQHSLKCAVFMSGGPPSFPDGTGVFLADEHGQIITMPTCHIIAYNDSLIDSAVALYHLCDQASATIVDHGKGHLVPRDPKSSKLMVKGIRDLIVDCYTMAFAVGLDLAFAPHHKANVGVIEIEFANVFEVEARTHGQTEQPMGFPQAWVYYNRSEAVSTSSKSRLELCASLGDLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCH8</accession>
    <name>A0A8H6CCH8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex II-specific subunit 65 IgD3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150369.1">
        <property type="nucleotide sequence ID" value="XM_037293540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCH8"/>
    <dbReference type="GeneID" id="59331027"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055420">
        <property type="entry name" value="IgD3_Trs65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024662">
        <property type="entry name" value="Trs65"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28159">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX II-SPECIFIC SUBUNIT 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28159:SF1">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX II-SPECIFIC SUBUNIT 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12735">
        <property type="entry name" value="IgD3_Trs65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex II-specific subunit 65 IgD3" evidence="2">
        <location>
            <begin position="421"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="409"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="592" mass="64517" checksum="821F7B4D61D245A6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCI9</accession>
    <name>A0A8H6CCI9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150384.1">
        <property type="nucleotide sequence ID" value="XM_037293555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCI9"/>
    <dbReference type="GeneID" id="59331042"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="255" mass="27425" checksum="65A7A6542B76D6A7" modified="2022-01-19" version="1">MRSHLLEKAQAEKEKKKGGGPRARGWVFGLGWFYAVAGESEEGTGSDRADFRRLGVGVWGGEIGVGGWVDRRVVDRRRGGVNGGGWEMENVTARTGVVKNCGIGGVRSLPRWLALPELFARSALSLHPPHTDSVPRIHLAALMRSAYASTTSAPRYVIIIRSTIRQLLTHKLIRAPGMDHGSERGTMPSASEAAVDDGSASGAPGMDRGSTDTVQNGSAESADPSGFKIKSDSDGQNHYCRGRGCDRCDLARWRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDU2</accession>
    <name>A0A8H6CDU2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151025.1">
        <property type="nucleotide sequence ID" value="XM_037292486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDU2"/>
    <dbReference type="GeneID" id="59329972"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034777453" evidence="1">
        <location>
            <begin position="19"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="369" mass="41871" checksum="08C8B556F7277C3B" modified="2022-01-19" version="1">MYLLIVFLALCFCQSVSSVPENDVPSANSTKALFKRVDNPTYAIDGTCTRYPQVQDIVNDWFQTVQVTSHRIDLGRADPHFQYAFQVLFDTDPNSNTLYKFELVEEPITAYDFVELVTTSLASLTPHADPDTKANLIFYCDDDAMNPTPYSNERWQLVPDIPGDPNPNSGRTLGINQEWFDQVNSISRTPSTKGCKDVANARGEPTELETYNDPITPFLYGNRLPPVGILQPTRSTITICDCVLVANNPYYPTIASSLTATLNRPRRQSPLRLYRPEYGRIVSAGLYHESTHWMDLKLEDEILRDGSIAYGWSKIMELNTFERLTNADTYMYLGLLARYESLGWWLDRHEGRAYLGVMVPAPAGWIPPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE41</accession>
    <name>A0A8H6CE41_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sterol 24-C-methyltransferase</fullName>
            <ecNumber evidence="13">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Delta(24)-sterol C-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_001676</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the transfer of methyl groups from S-adenosyl-methionine to the C-24 of sterols.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Steroid metabolism; ergosterol biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12 13">Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151143.1">
        <property type="nucleotide sequence ID" value="XM_037292604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE41"/>
    <dbReference type="GeneID" id="59330090"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003838">
        <property type="term" value="F:sterol 24-C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000121">
        <property type="entry name" value="Sterol 24-C-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050447">
        <property type="entry name" value="Erg6_SMT_methyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030384">
        <property type="entry name" value="MeTrfase_SMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013705">
        <property type="entry name" value="Sterol_MeTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068:SF1">
        <property type="entry name" value="HYPOTHETICAL LOC100005854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068">
        <property type="entry name" value="ZGC:194242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08498">
        <property type="entry name" value="Sterol_MT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51685">
        <property type="entry name" value="SAM_MT_ERG6_SMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 12 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 13" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="6 13" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="8 13" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase Erg6/SMT-type" evidence="14">
        <location>
            <begin position="80"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6221708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="377" mass="41969" checksum="78EF0793EFF64DA2" modified="2022-01-19" version="1">MAPIALENEDHSRDAAFNKAMHGKSVDSKGFRSMLNKDHDAHKAATDEYFKHWDNKSAGTETVEVREARKAEYATLTRQYYNLATDLYEYGWGGSFHFCRFAYGEGFHRAIARHEHYLAHTMGLRQGMKVLDVGCGVGGPAREIAKFAGVNIVGLNNNDYQIERAGKYAAQEGLSDHLSFSKGDFMQMSFPAETFDAVYAIEATVHAPSLEGVYSQIFRVLKPGGVFGVYEWFMTDRYDNDNPHHREVRLGIEQGDGISNMVRVSEGLEAIKAAGFELEMHEDLADRTDARPWYYPLDGDLRMASSLGDVLTIARMTRIGRFLAHNAVGGLERIGIAPKGTKRTADSLGYAADCLVAGGKEKLFTPMYLMIARKPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CED0</accession>
    <name>A0A8H6CED0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151347.1">
        <property type="nucleotide sequence ID" value="XM_037292808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CED0"/>
    <dbReference type="GeneID" id="59330294"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="68" mass="7596" checksum="9F6CDC3EE0401193" modified="2022-01-19" version="1">MCIEEGGFADPKEELERLVGKRHHEAATLRVRCSDGRYCFGNDTGAMLRLLQAKGSVDTISAQWILED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CED9</accession>
    <name>A0A8H6CED9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001966</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151433.1">
        <property type="nucleotide sequence ID" value="XM_037292894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CED9"/>
    <dbReference type="GeneID" id="59330380"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343:SF10">
        <property type="entry name" value="RNA-BINDING REGION RNP-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343">
        <property type="entry name" value="SERINE/ARGININE-RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="299" mass="31710" checksum="567FF99B40BB1C16" modified="2022-01-19" version="1">MSSTVVHVKNIAHNTSEKEVRDFFSFCGKITSLSVTPASEAENSPQSATVTFEKETAAKTALLLDNTQLGSSQVQVSSAAGISELAGTNTEDASRDEHDISQEDKPRSRIVAEYLAHGYVISDTAIQRAIALDNKHGVSNRFTNALTQFDAKYKATDRAKGLDQSYGITDKATAGWRGINSYFEKAMGTPTGQKLASFYTQSDKQVRDIHAEARRLADLKSGKGGGSMEQNEKTMEHVPGTEKTTCQCGGDTGTCPCAEGKCACSGCSKAGIHGEKTATGPADVVADTSGVQPLGEKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEF3</accession>
    <name>A0A8H6CEF3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nuclear pore protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001692</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin interacting component (NIC) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151159.1">
        <property type="nucleotide sequence ID" value="XM_037292620.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330106"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007231">
        <property type="entry name" value="Nucleoporin_int_Nup93/Nic96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225:SF4">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93 NUCLEOPORIN NUP93 DEAD EYE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04097">
        <property type="entry name" value="Nic96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="15"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="929"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1281"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="354"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="937"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1336" mass="146150" checksum="6EDF2259C84A7319" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF49</accession>
    <name>A0A8H6CF49_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151807.1">
        <property type="nucleotide sequence ID" value="XM_037292388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF49"/>
    <dbReference type="GeneID" id="59329874"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05120">
        <property type="entry name" value="APH_ChoK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF15">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="501" mass="58756" checksum="A911628DDA9B0287" modified="2022-01-19" version="1">MAEEIIEHFQTAERDYTVTRTRFIKRELKPSEYHKRQFGNFGTIVPRRVRERLENEKDAYRFVAEHTKIPVPKVLDFLEEDGICTLIVERVEGNIMEIVLDELDGEDRKRLIHNVTAFVEQSVLPQLRKFTSNRLGGVSGLVIPPNRVTFRDQRPEWRSRTFSENQFTFCHNDLARHNIMVNPKTLEVTAIIDWESSGFYPAEFEADMWSRSYRDYVPDEASTDRLIRLLDQPSAENFPVTMIDIPDGVIYMWQTEDRDRHCTLTPSTFTKRQLRPYEKSLGVQGTITPFPWSRERLKNEKKALEFIAEHTSIPVARVHAFNEEAGISSLTTKIPVPGARMDILARRLSNRDKAILLTHVNTFVNQIVLPQLNTLRSSKLGGKIGMTIPPIRVWKRDKRLSWPARTSNTNRYVFCHNEISQEYIWVDPKTLQVVLLQNWECSGYYPPEFEYPYWRKARDEWMNNDDEVDRLILFLDKPGKGSESRLFDRTMLRTLQMNNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF74</accession>
    <name>A0A8H6CF74_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001364</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151713.1">
        <property type="nucleotide sequence ID" value="XM_037292294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF74"/>
    <dbReference type="GeneID" id="59329780"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="300" mass="32920" checksum="BCD3BAC2F3343411" modified="2022-01-19" version="1">MEEGKKLTSRPGNGEPVYQDCLRALDEFDTFEDDEDESSTAAFLGIGKRASYIALYGEDPLGQEGIYQLPVVKTNGPCSIAVAQTIYTRHLFHSEYWSVLQGKANGVVELCAKTQGIGGWHETVMVKRLRWLHTFIIMSSSSEECSEYKWHVRPTPKVALYARTPGTNRNHLPRIQQPEEATGMLKAGAMRDKAHPLTLNLPPDNIAMNAVLNPMTTITPMAASASMQNCNYVPPACCLSGNGIVFEDPSEKLAMTVQLPNTSVCCHETTEKWRNSSTARDSAVEDPACPVAIVQGVTNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF78</accession>
    <name>A0A8H6CF78_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COP9 signalosome complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001207</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the COP9 signalosome (CSN) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family. CSN5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151556.1">
        <property type="nucleotide sequence ID" value="XM_037292137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF78"/>
    <dbReference type="GeneID" id="59329623"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000003">
        <property type="entry name" value="COP9 signalosome complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040961">
        <property type="entry name" value="CSN5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18323">
        <property type="entry name" value="CSN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="10">
        <location>
            <begin position="51"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="339" mass="37790" checksum="01B080B68EE2A9AE" modified="2022-01-19" version="1">MDRFQKVWELENAAKLVDPQRDALYAYDDAEQKSISNEGPWKKDPHHFKNVRISAVALLKMVMHARSGGSIEVMGLMQGKISGDAFIVTDAFRLPVEGTETRVNASDQANEYVVQYLASCRENGRAENAIGWYHSHPGYGCWLSGIDVDTQLTQQMFQDPFLAVVIDPDRTISAGKVEIGAFRTFPKDYQPAASGGGDGYQTIPLAKIEDFGAHASRYYSLEISHFKSTLDSHLLELLWNKYWVSTLSQSPLFTNREYGSKQMLDLSEKIRQAEKNVKAGSRGGQHRFNGQDTQLDKVIRDSNKIAGEEVTGLLVSEFKAKLFNTVGATIAREDNASKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFA4</accession>
    <name>A0A8H6CFA4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151839.1">
        <property type="nucleotide sequence ID" value="XM_037292420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFA4"/>
    <dbReference type="GeneID" id="59329906"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022025">
        <property type="entry name" value="Amidoligase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36847">
        <property type="entry name" value="AMIDOLIGASE ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36847:SF1">
        <property type="entry name" value="AMIDOLIGASE ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12224">
        <property type="entry name" value="Amidoligase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="358" mass="40617" checksum="3B6E48B7D38E8E55" modified="2022-01-19" version="1">MAVLSLRIGIETEVLLCAIDMQDQCKGSLEEFAKGLVGHYNTKVRERPGQPEMRMAFDTWHASEDPTNSRYWSVVEEISIDPDEDHWGLEFASPIIRYEAGSSWRQNLHYHFQIIESYAEIMSDVTCGTHVHVSPGYSKWTLEQVKNVSRSILYFEEAFEVLLPPGRRGNRECRSNRFDNPKLKHLPDLESCFQRIQECTAIESLIHLMNAKEEDPSNVCEDSNGEKAVTERRYGFNFENLMEGKIGTIGMHPICGEMPTECTECSPPETEFRRPPCVTNYQDCVMWAEFGASFMQAAMQYGTSPDHLRTYGPDVAGLAAFIGQAAIPGMNDRAILEKMFARGSGCLPVGALKYHHYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFT8</accession>
    <name>A0A8H6CFT8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000828</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152126.1">
        <property type="nucleotide sequence ID" value="XM_037291764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFT8"/>
    <dbReference type="GeneID" id="59329246"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321:SF8">
        <property type="entry name" value="ESTRADIOL 17-BETA-DEHYDROGENASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="249" mass="26683" checksum="B8BDD30CDE786A88" modified="2022-01-19" version="1">MGLATAKALFARGASLSLADYREDALTQTRKEIESSFTSTSSAKPNILATAVDIRESSQVDSWIEKTISHFGRLDGAANIAGVLGKNFGVHILVQLSNEEWEFVTRTNFTGLFYCMRAQSRVLSDGGAVVNAASTTGLEGHPKNSPYSATKHAVIGLSKSAAGEVGGRGIRVNCVAPGIFNTPMVAGLGGVESAKLEMFNRVPLSRIGQPEEAASVRAFLLSDGAKYSRSSPDPKSRLERLREIRNMYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHC4</accession>
    <name>A0A8H6CHC4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152699.1">
        <property type="nucleotide sequence ID" value="XM_037291264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHC4"/>
    <dbReference type="GeneID" id="59328744"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="171" mass="19769" checksum="5E6E40AB08AD5483" modified="2022-01-19" version="1">MDYLFAAWTVIYFIAYPIFYILKFILFLSAIITAPLLHLGHYCLYACWYVLRVLGKFETLYIFFGIATLVGLVTGTSLHYVSGFITSILNLDSLPEQQRGRTLASYRAEKQERRDAKDPIMELRQKDEGLQGNDLRLREDYMNWSLSKQGRGRGGPRFNTILEEEDTSDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHP1</accession>
    <name>A0A8H6CHP1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spindle pole body-associated protein cut12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000368</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152742.1">
        <property type="nucleotide sequence ID" value="XM_037291307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHP1"/>
    <dbReference type="GeneID" id="59328787"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021589">
        <property type="entry name" value="Cut12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11500">
        <property type="entry name" value="Cut12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spindle pole body-associated protein cut12" evidence="3">
        <location>
            <begin position="189"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="490"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="563"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="598"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="294"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="399"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="624"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="648"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="679"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="726"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="737" mass="80686" checksum="72219529A67F70A1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI61</accession>
    <name>A0A8H6CI61_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010523</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153009.1">
        <property type="nucleotide sequence ID" value="XM_037301375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI61"/>
    <dbReference type="GeneID" id="59338913"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="105"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="375"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="376"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="433" mass="47496" checksum="E0D0A39E7C318DC9" modified="2022-01-19" version="1">MASPPPELIKLAAQYVLTWPSCIQPDAIKGMIASGCPSDPKCFCEDKIFRLELLAGITTNCTAADMLTTQSLAKKLCPVLDESREATVIAVMVVITLLALVLVALRIVSRKIGHVKFWWDDYLIFFVMLVTLGETANILSGVVNGIGKHIVTLGASHMTGFLKNFWITQVLFAFSMTGLKMSILLFYRRLFTGRPMAFTLILAGCFNLAWFVAQFVSVMVSCRPLAYFWDKAIPHGKCIDENLASYLITGVSLLMDVIIFLIPIPPLWGLKKSLAQRLSLIGLFLAGALVCVAGAVRIPFLVEMDLTDITWSIVPAGLWIQVEVKVGIVCACLPTLPPLFRRVRQYLLLLSSGSGNSERSPSSGDKNGGSVVVAVDDARTGDASPNREGRGRKQQGWYSQMLNSLSNKSAEGQTESREEIVEVHERKFSDDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIN5</accession>
    <name>A0A8H6CIN5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NFACT RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010764</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153249.1">
        <property type="nucleotide sequence ID" value="XM_037301615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIN5"/>
    <dbReference type="GeneID" id="59339154"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039730">
        <property type="entry name" value="Jlp2/Ccd25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049">
        <property type="entry name" value="DUF814-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="3">
        <location>
            <begin position="15"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="188" mass="21703" checksum="9EA34B546EDC70F4" modified="2022-01-19" version="1">MVYYFTSNVIDPPATIYFHADNLSSAHIYLRLTPEQTWDALPKELVEECAQLTKANSIEGNKKDNVTIVYTPWANLHKNAGMATGQVGFRDPKRTKKTLVPTRLNPTINRLEKTRRARPVDDLPADKEAHLARQRATKRAENERRQKEADGVARERREEKERRERGYEDLMAEGGRSNEEGFDEDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJI7</accession>
    <name>A0A8H6CJI7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="16">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_011092</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits. Binds 90S pre-ribosomal particles and dissociates from pre-60S ribosomal particles after processing of 27SB pre-rRNA. Required for the normal formation of 18S rRNA through the processing of pre-rRNAs at sites A0, A1 and A2, and the normal formation of 25S and 5.8S rRNAs through the processing of pre-rRNAs at sites C1 and C2.</text>
    </comment>
    <comment type="function">
        <text evidence="16">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of pre-60S ribosomal complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="16"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153575.1">
        <property type="nucleotide sequence ID" value="XM_037301941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJI7"/>
    <dbReference type="GeneID" id="59339482"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17960">
        <property type="entry name" value="DEADc_DDX55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056330">
        <property type="entry name" value="CTT_SPB4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23681">
        <property type="entry name" value="CTT_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="51"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="292"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="586"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="596"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6224515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="640" mass="71204" checksum="935BB06DF724229C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKY0</accession>
    <name>A0A8H6CKY0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin carboxyl-terminal hydrolase 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153868.1">
        <property type="nucleotide sequence ID" value="XM_037301051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKY0"/>
    <dbReference type="GeneID" id="59338588"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="62123" checksum="034C470E08EFC8D2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLD0</accession>
    <name>A0A8H6CLD0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154338.1">
        <property type="nucleotide sequence ID" value="XM_037300490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLD0"/>
    <dbReference type="GeneID" id="59338024"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="257" mass="29213" checksum="E4570182210BBBD5" modified="2022-01-19" version="1">MTQPFDIYIISSNSQIIRDFTAEQNHAFTNILLPALQVVLRQTIDKHFDPLQPQQKQSIIAPKPAQQEQEQKYRPLQQAKMEKTAEKSTKKITETVSSSSDTTGLLSTVSHQPTQSATQPEHSVKQEEKPLQAITENTAKKSSDNKATIIKKTAQLSKIPRYILSTIPRQIATQQAENDTTIWPAISACLGNITLVWHITARSKQEKDLLRRLQPNYYDQQAKATHISLTSHRIAIRSMRKMRRRIRSITASSNDTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLF7</accession>
    <name>A0A8H6CLF7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009552</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154261.1">
        <property type="nucleotide sequence ID" value="XM_037300413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLF7"/>
    <dbReference type="GeneID" id="59337947"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="142" mass="15403" checksum="8E098C885ED40E8E" modified="2022-01-19" version="1">MPSPSATNFNRDKEIPQLTNFCTSLNSKYFLLGEPTTEETPTSILDAAAGVSVTAFSSSSDYKGRAIGSDAVSIEECVGNFLPVIHPSKSNIDRIDRRASTDGMNNGYLQNTSNLLHRIIGNHLERQTFTTCATWSTSVPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLY1</accession>
    <name>A0A8H6CLY1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009669</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154378.1">
        <property type="nucleotide sequence ID" value="XM_037300530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLY1"/>
    <dbReference type="GeneID" id="59338064"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010634">
        <property type="entry name" value="DUF1223"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36057">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36057:SF1">
        <property type="entry name" value="LIPOPROTEIN LIPID ATTACHMENT SITE-LIKE PROTEIN, PUTATIVE (DUF1223)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06764">
        <property type="entry name" value="DUF1223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="220" mass="24364" checksum="2E793B4A0BF04700" modified="2022-01-19" version="1">MSLRRIYTLTVVVRRAHQKSNVLKLAEDPNMLILTCHVTYWDRFGWKDIFAKSAFDQRQWEYATALRRKNVFTPQVIVNGQVDGVGHNSRDLQVLITKGNAFSTAQTMEILYMIHGGGITVSGLGNEHGVVSVIRYDDRDHDGSITHGENNGRKLRHSHIVKDVTHVGSWSGGFQSYALPPLQRGGEKVAVLVHAGAGGPIIGAARIKEAEGLDLREDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM18</accession>
    <name>A0A8H6CM18_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit tim50</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_008991</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to direct preproteins in transit and direct them to the channel protein TIM23, and possibly facilitates transfer of the translocating proteins from the TOM complex to the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154678.1">
        <property type="nucleotide sequence ID" value="XM_037299852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM18"/>
    <dbReference type="GeneID" id="59337386"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000019">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM50"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0811">Translocation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="16">
        <location>
            <begin position="310"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="33"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="587"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="592"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6226125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="608" mass="67865" checksum="91EFAB5CD4DFAAB0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMC0</accession>
    <name>A0A8H6CMC0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rho1 guanine nucleotide exchange factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009768</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154475.1">
        <property type="nucleotide sequence ID" value="XM_037300627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMC0"/>
    <dbReference type="GeneID" id="59338163"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04435">
        <property type="entry name" value="DEP_fRom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041675">
        <property type="entry name" value="PH_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF2">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15405">
        <property type="entry name" value="PH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="5">
        <location>
            <begin position="526"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="754"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="6">
        <location>
            <begin position="907"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="41"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1228" mass="138985" checksum="F99998395F261A42" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMD6</accession>
    <name>A0A8H6CMD6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154858.1">
        <property type="nucleotide sequence ID" value="XM_037300032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMD6"/>
    <dbReference type="GeneID" id="59337566"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="127" mass="13331" checksum="C7226340473C99C7" modified="2022-01-19" version="1">MVATLVESCGGAVLGDGADVGVRIGDVLGGKVEVVEVINVVSWGGLVVLGRPCGARGHQSNELQYGNAGEGALMMICNDVPPRTSIKPVAEPLLNVRKSEVTETAKSHAIRTMLIFYDLRIMALDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMR2</accession>
    <name>A0A8H6CMR2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008954</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154642.1">
        <property type="nucleotide sequence ID" value="XM_037299816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMR2"/>
    <dbReference type="GeneID" id="59337349"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF5">
        <property type="entry name" value="HYPOTHETICAL ACETYLTRANSFERASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="287" mass="31584" checksum="CEE132386D867FAB" modified="2022-01-19" version="1">MHPPQAVWELMFPIVSHGPNAEAEAIKKGSAVQLQGSRTGPHDQWIKVSDTDTGKIVTGALWKFYNSNPYRVPFGEFDPTWFSEGEPRDLCNSMNTQLRAWRPETMAAPHALLKILFTHPEDRNRGVAGLLVEWGTRKADKRGLEAYVEEGQLVHLHLDLADLSTIKASAEAFLAKNTRLDIVFNNAGVLLPPQGSKTAQGRELQIGTNCLDLLHFTKLLRPMLAVTAKTAAKSSIRVVWVSSNSAKISSPQNGIDMTDIDYHIDKNAVTKCGTSKASDIFHSSESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMV4</accession>
    <name>A0A8H6CMV4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009305</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154992.1">
        <property type="nucleotide sequence ID" value="XM_037300166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMV4"/>
    <dbReference type="GeneID" id="59337700"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF5">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22061">
        <property type="entry name" value="CSN7_HB_subdom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="203"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="256"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="272" mass="29834" checksum="A4AFCF716D93A8E9" modified="2022-01-19" version="1">MDQTQQKALNALEPYILLSKSANSPRAAADLVTQATSAPNTFVFAELLQTPNIQALQTASKEYAPYLTLLHIFAWGTWSDYTETTNLPKLSNVQEQKLRQLTLLSLPTSPTTLTYAHLLSELSLSTTRALEDLVISSIYAGLLTAKLDTLSQRVDVSSVAPLRDLRPGSVPRMVSVLEDWDTRCVSVLGEIEGQVREIRRKAQQQRKRETEHEKAVAKKMGENEDGKGKLGKRGGGEEADEMDIDESGRKLRAKRGGGNFLGGLGRRLGGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPD3</accession>
    <name>A0A8H6CPD3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methionine aminopeptidase</fullName>
            <ecNumber evidence="10">3.4.11.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_008430</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with zinc, cobalt, manganese or divalent iron ions. Has high activity with zinc; zinc cofactor is transferred into the active site region by the ZNG1 zinc chaperone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associates with the 60S ribosomal subunit of the 80S translational complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155297.1">
        <property type="nucleotide sequence ID" value="XM_037299296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPD3"/>
    <dbReference type="GeneID" id="59336826"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031615">
        <property type="entry name" value="Zfn-C6H2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330:SF7">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15801">
        <property type="entry name" value="zf-C6H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52013">
        <property type="entry name" value="ZF_C6H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C6H2-type" evidence="12">
        <location>
            <begin position="8"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="364"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>a protein</name>
            <dbReference type="ChEBI" id="CHEBI:16541"/>
        </ligand>
        <ligandPart>
            <name>N-terminal L-methionine residue</name>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>a protein</name>
            <dbReference type="ChEBI" id="CHEBI:16541"/>
        </ligand>
        <ligandPart>
            <name>N-terminal L-methionine residue</name>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6226989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="394" mass="43085" checksum="D9507DDBD2ED0903" modified="2022-01-19" version="1">MAEVVTGAKNCIGVGCPKDAGALQCPTCLKLGKESSFCSQDCFKRSWSEHKSVHKSGSIALHNPFPTFPYTGPLRPIYPLSDRRTVPESIPHPNYAKTEGGNPNYKFAARNSITCLDKKQQAGMRKVCRMAREVLDVAARELKPGVTTDYIDEVVHKECIKRDSYPSPLNYSGFPKSVCTSVNEVICHGIPDQRMIREGDIINLDVTLYHGGFHGDLNETYYIGEKALKDRESVRVVETARECLEEAIKLVKPGMAFKDPGTVIEKHAKSKGCGVIRTYCGHGINSLFHCAPNIPHYDKNKAVGIAKPGMTFTIEPMISLGSFRDKTWPDDWTSVTLDGSRTAQFEHTLLVTEDGVEVLTARLADSPGGPVEMPPELKADGDGAANGASDKVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPG5</accession>
    <name>A0A8H6CPG5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155602.1">
        <property type="nucleotide sequence ID" value="XM_037299601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPG5"/>
    <dbReference type="GeneID" id="59337132"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="568"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="648"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="731"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="835"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="996"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1138"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="731"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="835"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="855"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1075"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1152"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1182"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1220"/>
            <end position="1244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1273" mass="139362" checksum="8D6F5C9C9E01476B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPT9</accession>
    <name>A0A8H6CPT9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155567.1">
        <property type="nucleotide sequence ID" value="XM_037299566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPT9"/>
    <dbReference type="GeneID" id="59337097"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18785">
        <property type="entry name" value="Inv-AAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="182" mass="19873" checksum="B6EF65AE3D1BDE5D" modified="2022-01-19" version="1">MASEPPHPTISAGDHKAYMEYALERARLSPPASTKFCVGAVLVDADENVILSTGYSLELPRDSLGDPGSTHAEQCCFIKVAQKHNLPEERIGEVLPKNTVLYTTMEPCNKRLSGNRTCVERILRLNGAIKVVYVGIREPEVFVGENVGMQKLKDAGVIVVVVEGMQDRILQVSTAGHEVEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPX4</accession>
    <name>A0A8H6CPX4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155736.1">
        <property type="nucleotide sequence ID" value="XM_037299735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPX4"/>
    <dbReference type="GeneID" id="59337267"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06532">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002654">
        <property type="entry name" value="Glyco_trans_25"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01755">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 25" evidence="2">
        <location>
            <begin position="143"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="490" mass="56194" checksum="8E24958172967781" modified="2022-01-19" version="1">MQHYIDLQWLDARLEKLKLYLEELKVNLVHLEPQCKYLRPRFQDVESHLEYAMPQMNDFKPNFRRLGISGRTQRSLSQKLLVFGLIVLFIVSWSNFLRQGVSSSTSRRAESENSVFNDTLGVCVPAMSLYWEVLADIGEKFQHVFALALPDRLDRVQPLLNAASATNINVTILNAVRDKQIPEDAWPKGWSDNHEKKSGELGCLTSHVRTWKKMIAENIESALIMESDTDWDLRIKETMQGVASASKRLVDWPFDVPKRPGIFPYGDKWDVIWFGHCGAWNHENHRIYSFNDSAVPPEDYEYTFSDKPREEQHRPGTRSVFPLGFAVCSTAYAISNAGAVKLEKEFREGSDNIDLRLATICRENQDVNCLGVWPQVFTAAITKTNIEHPLGEIVTGETAVTEWYPGLGLQYSARLNADKVLNGSSTEDEWDPQWNSTWALKNETWIQMGFEEAKKLEAEKTMSDDHIEDNGKRNFWQLYNGRYAGTRSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQR3</accession>
    <name>A0A8H6CQR3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155858.1">
        <property type="nucleotide sequence ID" value="XM_037298522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQR3"/>
    <dbReference type="GeneID" id="59336049"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034500992" evidence="1">
        <location>
            <begin position="21"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="232" mass="24413" checksum="EFB8DD9B905F9B4A" modified="2022-01-19" version="1">MLYLSFAALGLTFFSLNAAAVPLASPEAIASIMANLPAEHAAGLASLMARGNNPPTFNSSIDVLQNSSMSAIIDSYIANLEQAGFVPNANATISKRDALSLDSLVEKRSVPSIPLVINLLEQHGFVPVSATDGTASKRDASGLESALEKRTLPDINLVISRLEAHGFVPSGNSSQSSISKRNNASDINTVISQLEAYGFNPNDFMRGNFSASANSTTSFPKRAAHMSLKEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRI9</accession>
    <name>A0A8H6CRI9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007982</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156186.1">
        <property type="nucleotide sequence ID" value="XM_037298850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRI9"/>
    <dbReference type="GeneID" id="59336379"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000266">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034886317" description="Carboxylic ester hydrolase" evidence="3">
        <location>
            <begin position="18"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="62"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="612" mass="65952" checksum="FCA8E262BC55B0B0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRN3</accession>
    <name>A0A8H6CRN3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trimethylguanosine synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cap-specific guanine-N(2) methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008068</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78479"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="Rhea" id="RHEA-COMP:19089"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="Rhea" id="RHEA-COMP:19090"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:78508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78471"/>
            <dbReference type="Rhea" id="RHEA-COMP:19085"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:78472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78475"/>
            <dbReference type="Rhea" id="RHEA-COMP:19086"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78476"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156272.1">
        <property type="nucleotide sequence ID" value="XM_037298936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRN3"/>
    <dbReference type="GeneID" id="59336465"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="277" mass="30934" checksum="35130C9B26034B9A" modified="2022-01-19" version="1">MDGGLWEGDCPELSCMDIETKDDSKAMAPPRGCQHYANLTEMPWDIQKYYHQRFSIFSKYDEGIWMTEDAWFGVTPEPVASTIAKHIAGAASAGKAILIDCFAGVGGNVIAFAQSERWKRVYAIEKDSRALACAKHNAEIYGVRDSISWYEGDCFEILRNELAELGQHSILFASPPWGGLSTLLRLVRHEASLMNAGPGYRSDTVFDLAKMQPYTLTDVLHPFQQLTEDVVLYLPRTSDVRQLANKSGKGRKTTVMHYCMEGASKVGRTYRSGPSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CTZ2</accession>
    <name>A0A8H6CTZ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_006967</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156498.1">
        <property type="nucleotide sequence ID" value="XM_037297863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CTZ2"/>
    <dbReference type="GeneID" id="59335367"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17973">
        <property type="entry name" value="DEXHc_DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000324">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000003">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044756">
        <property type="entry name" value="DHX15_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF109">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="202"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="389"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6228856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="858" mass="97075" checksum="5BE5F406BF54DB46" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7C9</accession>
    <name>A0A8H6F7C9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000028">
        <property type="protein sequence ID" value="KAF6217443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146878.1">
        <property type="nucleotide sequence ID" value="XM_037297809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7C9"/>
    <dbReference type="GeneID" id="59335313"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034906502" evidence="1">
        <location>
            <begin position="20"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="111" mass="11579" checksum="BD082CFFA57AFFFC" modified="2022-01-19" version="1">MKSFTISFALLCFITSSHAAPHSAHAEPRQFEAGITFIGAGPDPPSYFQAFPTDDTLHTINNSLSVSQISSAGGAICSFFGVDGSDTFIHGGETVDVGPPQVQVSGVCDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7P8</accession>
    <name>A0A8H6F7P8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147481.1">
        <property type="nucleotide sequence ID" value="XM_037297356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7P8"/>
    <dbReference type="GeneID" id="59334859"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25586">
        <property type="entry name" value="zf-CCCH_PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="2">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="13222" checksum="5BAB2DCDEAEC20B5" modified="2022-01-19" version="1">MSAFIRPHSECLNSRARIRQNGNGRENAKDTLCRNVTIYGHCRYEDKGCAFNHDPVKSQPNSQVESIKKRFNIASPSFTPSSLAVNGNHATPKTLGLSPKAANAAPFKPRTLTPGNIVLMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7U5</accession>
    <name>A0A8H6F7U5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147373.1">
        <property type="nucleotide sequence ID" value="XM_037297248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7U5"/>
    <dbReference type="GeneID" id="59334751"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.400.10">
        <property type="entry name" value="Acetoacetate decarboxylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023375">
        <property type="entry name" value="ADC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40518">
        <property type="entry name" value="ACETOACETATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40518:SF1">
        <property type="entry name" value="ACETOACETATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160104">
        <property type="entry name" value="Acetoacetate decarboxylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="345" mass="37956" checksum="B0B1C194E0078941" modified="2022-01-19" version="1">MSTSAPSPEISLPVAPPPWTCKATVYVLSFYHFASSALPLDIVYDPLELHAASFSSEKETGKYTGGLGFAQIVRYSESPVGTYDELAILPGFFSSIGPDGKKGKDSRITGIWVSQEATLMNGRRNWNIPKHLARFSFTPSDPSTSSPVKVEVFRADPSSTQPFFSATIHPISYLPFFSMSSTWLSYLGISTHILQPPLPQGDPSGVVVRSEDWKRSHPKLKTSRAKMVWIDMKQSDEGCKAAETAVGQGEGDPLSTKHEKVYENWWPGMRRVSDSPLASPLIAIGSSFISVVNSLLMFAFVHFLTIIILQGNAEKFRSSVDDQNVEVNASIRLSVYGKDQSLEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F869</accession>
    <name>A0A8H6F869_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005400</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148292.1">
        <property type="nucleotide sequence ID" value="XM_037296310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F869"/>
    <dbReference type="GeneID" id="59333806"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009316">
        <property type="entry name" value="COG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024602">
        <property type="entry name" value="COG_su2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06148">
        <property type="entry name" value="COG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 2 N-terminal" evidence="11">
        <location>
            <begin position="26"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="42"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6218857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="281" mass="32092" checksum="289ECAEFDF4BAFB7" modified="2022-01-19" version="1">MSKRFYFGGDDSSASSDDNLPYPKPLTRASFLTPSFDPATFLSTLHNRHQTLEDLRAELRTRSQDLNKELLDLVNENYQDFLSLGSSLKGGDEKVEEVRLGLLGFRREVEGLRGKVDGRRQEVEELVHERKRIRESVQVGRRLLEIERKIGELEGRLMLASTIPQKAAVNEEEVIPSESEEESDEEESNGISIPRLQRHMEQYLYIKGSMERIGPEHPFLIKQEERVLRLKQTVLLDLNSALKQAVAGGENMRGELLKLLGIYKQMGEASEAMQVLKESKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8E1</accession>
    <name>A0A8H6F8E1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ArsA/GET3 Anion-transporting ATPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005903</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the arsA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147988.1">
        <property type="nucleotide sequence ID" value="XM_037296807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8E1"/>
    <dbReference type="GeneID" id="59334308"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000235">
        <property type="entry name" value="ATPase ASNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03112">
        <property type="entry name" value="Asna1_Get3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027542">
        <property type="entry name" value="ATPase_ArsA/GET3_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="10">
        <location>
            <begin position="26"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="327" mass="36171" checksum="AAF7C9B127134F11" modified="2022-01-19" version="1">MSATVDADSEDLAPTLESIIHQKSLRWIFVGGKGGVGKTTTSCSLAIQLARVRKSVLLISTDPAHNLSDAFSQKFGKEARLIDGFTNLSAMEIDPNGSIQDLLKEGGEGAEEIMAQTGGMGGMMQDLAFSIPGVDEAMSFAEVLKQVKSLSYETIIFDTAPTGHTLRFLQFPTVMEKALAKISQLSTQFGPMLNSVIGARGGLPGGQNLDEVIAKMETLRETIGEVNTQFKNPDMTTFVCVCIPEFLSLYETERMIQELSSYQIDTHAIVVNQLLFPKKNNPCEQCNSRRKMQKKYLEQIEELYDEFNVVKMPLLVEEVRGKEKLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8E6</accession>
    <name>A0A8H6F8E6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1 9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005798</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1 9"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147884.1">
        <property type="nucleotide sequence ID" value="XM_037296703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8E6"/>
    <dbReference type="GeneID" id="59334203"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075018">
        <property type="term" value="P:positive regulation of appressorium formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07849">
        <property type="entry name" value="STKc_ERK1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000073">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="355" mass="41074" checksum="BEEF3C544BC65BA9" modified="2022-01-19" version="1">MSRQQPAPPGGSRKISFNVSDQYDIQDVVGEGAYGVVCSALHKPSGQKVAIKKITPFDHSMFCLRTLREMKLLRYFNHENIISILDIQKPRNYDTFSEVYLIQELMETDMHRVIRTQDLSDDHCQYFIYQTLRALKAMHSANVLHRDLKPSNLLLNANCDLKVCDFGLARSAASTEDNSGFMTEYVATRWYRAPEIMLTFKEYTKAIDVWSVGCILAEMLNGKPLFPGKDYHHQLTLILDVLGTPTMEDYYGIKSRRAREYIRSLPFKKKIPMKAMFPKTSDLALDLLEKLLAFNPVKRITVEEALRHPYLEPYHDPEDEPTAEAIPEEFFDFDKNKDTLTKEELKQLIFAEIMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8F3</accession>
    <name>A0A8H6F8F3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004937</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148547.1">
        <property type="nucleotide sequence ID" value="XM_037295849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8F3"/>
    <dbReference type="GeneID" id="59333343"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="9">
        <location>
            <begin position="141"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="334" mass="37091" checksum="648F1E69598A90A2" modified="2022-01-19" version="1">MKTFSPTPMRSVLQVTTRPRLGPLGHSSQLPSRLSKRSSQRYARAAQTFSPQPKKVLNLVLRRPAWSSHRSYNNNSYGYYTPESFWVRNKPNIVVGSIIGFCCVTYYYKWTAENLAAKGNHARADLIRRNFVNSGENLREGRWWVMISSSFAHGNLMHLGFSVLPIWGFGRPLVGLLGVPCFMGLCVISAVSSSRAYIYWEDTKETLRKEMFNRRWDKPKDPRVLGIQISKERALAISGSGGAHYGGSQGASGIACGLTGARVCIMPWAWTSYFSILPMPLWAGETLLTIASVVGMATGYAPIVGHAGHLGGTAVGIAYYYGIARPWLRRTGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8M3</accession>
    <name>A0A8H6F8M3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148471.1">
        <property type="nucleotide sequence ID" value="XM_037296489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8M3"/>
    <dbReference type="GeneID" id="59333986"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="130" mass="14608" checksum="46B98974591129E4" modified="2022-01-19" version="1">MDEWDRRERAQREQDRVRKLERKAKDAGNADFANTNNDNQREIIKKPEKPRQKATAKAGVTQKSRKWPKREKQQLSGAQLAPEVEQGRKGERKGQSQGSSSPIGGQHGYTTKFQIGVITTPHGGKDTSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F910</accession>
    <name>A0A8H6F910_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004904</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148514.1">
        <property type="nucleotide sequence ID" value="XM_037295816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F910"/>
    <dbReference type="GeneID" id="59333310"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="357" mass="40505" checksum="6F401B0B680883A8" modified="2022-01-19" version="1">MHRFRMDAKVAAIRSKIVDKVADKMAEQALTRSQARGDNDFDSEKFQPAALGDLARELTTLVGVCGPAAIAMLARDSRKDMDERYHVWPDRESTGLVTQERFFAQLVNETAAAHEKSWFNGTKENLLRLQIMSSYDQELVKGDWWASNGITFCATIYKSIRKELTIRTEELEKTLQGINDSAKQVYIARNHIVKCIKDSFSQLHQNVQNTCTTPKTRNNQVLQLCYTANKREQERCILHIDSLFKALIGEAARLPQEIIQSGIKSYMSQFYDKIQLYFADRKATQSRGEKDPGRVTEIVRGKLESFLAKEAPTMLRHAANAIKTALSTVVTDTISRATSTDRESTLDKAISNLQRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F937</accession>
    <name>A0A8H6F937_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein YIF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004000</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the YIF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148966.1">
        <property type="nucleotide sequence ID" value="XM_037294919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F937"/>
    <dbReference type="GeneID" id="59332409"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005578">
        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083">
        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF0">
        <property type="entry name" value="YIP1D-INTERACTING FACTOR 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03878">
        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6219531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="320" mass="35209" checksum="43E85428F4CF9E1C" modified="2022-01-19" version="1">MMRSPPPPQSQGGSNYGSPYGQMGGPAQQSGGGGPFAPAFGGFVNDQTAQMGFQVGQKAVMAGQEYMEQSINRYVSIPALKHYFNVSTPYVLRKILLVLFPWRHKPWSREQRPSSTAQDPSTPNTAYSYYFLPPRDDLNSPDMYIPVMAFVTYILLSTLLAGLRGAFHPEVMGLTATAAFAVVAVEILGLKFGTYILSISNDSQLLDLVAYSGYKFVGVIVTLVVAEILNRGKGTGGWVGWTVFTYTFLANAFFLLRSLKYVLLPDSSSTPAGGMQTVDRAQRNRRTQFLFLYSYVVQFVFMWVLSRQDSATATVVGIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9C4</accession>
    <name>A0A8H6F9C4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-ribulosamine 3-kinase</fullName>
            <ecNumber evidence="1">2.7.1.172</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003982</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>N(6)-D-ribulosyl-L-lysyl-[protein] + ATP = N(6)-(3-O-phospho-D-ribulosyl)-L-lysyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48432"/>
            <dbReference type="Rhea" id="RHEA-COMP:12103"/>
            <dbReference type="Rhea" id="RHEA-COMP:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:90418"/>
            <dbReference type="ChEBI" id="CHEBI:90420"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.172"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:48433"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.172" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148948.1">
        <property type="nucleotide sequence ID" value="XM_037294901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9C4"/>
    <dbReference type="GeneID" id="59332391"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102193">
        <property type="term" value="F:protein-ribulosamine 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016477">
        <property type="entry name" value="Fructo-/Ketosamine-3-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12149">
        <property type="entry name" value="FRUCTOSAMINE 3 KINASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12149:SF8">
        <property type="entry name" value="PROTEIN-RIBULOSAMINE 3-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03881">
        <property type="entry name" value="Fructosamin_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="359" mass="41442" checksum="E4580FE3BC51FCDA" modified="2022-01-19" version="1">MSDDGPALKEWPPVELIAGLSLETTAEPESELDEKVHSELLPGTEVLEITPSGASAWVQTVRIRTRQKDGTTKDYFKKSQSGSVGRRMMLGTFASEKAFYEYAPHNIPKPIAWGNYKSNEDMWFYLAEYHDMVDEVPDPQQFVSIIVKIHQESVGKSPTGKFGFDVPTHLANIPSDNGWEDTWEKFFTKAMKRMLELEELSHGKDDEEFEELKEKLLTKVIPRLLRPLESGGRKVTPCLVHSDLWPGNCMPDADTDEIMIFDSCAYWGHNESDLGSWRAPRYRMGKPFLREYQRRMGMSYPEEDWDDRNALYAMRYDILVSALFPNSDKFRNLARDEMKRMSEKYSNGFDDFEEVISQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9G4</accession>
    <name>A0A8H6F9G4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003746</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149356.1">
        <property type="nucleotide sequence ID" value="XM_037294668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9G4"/>
    <dbReference type="GeneID" id="59332157"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052355">
        <property type="entry name" value="CENP-V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620:SF1">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620">
        <property type="entry name" value="CENTROMERE PROTEIN V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="4">
        <location>
            <begin position="12"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="133" mass="15452" checksum="B5C92C5A3A991D80" modified="2022-01-19" version="1">MSNSESPAKVWYEANCHCGAIKYRVNVPSLETQKVTNCNCSICCKNGYLNVYPKRTDVVFHKGEDHMKGYLFGEKRCMHKFCPTCGSSLFVDPHMDDPELIVVNVRMFKDIDVDKLELWKYDGFNRLQPRYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9X9</accession>
    <name>A0A8H6F9X9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentatricopeptide repeat-containing protein-mitochondrial domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003043</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150045.1">
        <property type="nucleotide sequence ID" value="XM_037293967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9X9"/>
    <dbReference type="GeneID" id="59331455"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140053">
        <property type="term" value="P:mitochondrial gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057027">
        <property type="entry name" value="TPR_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF35">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23276">
        <property type="entry name" value="TPR_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Pentatricopeptide repeat-containing protein-mitochondrial" evidence="3">
        <location>
            <begin position="332"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="474"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="608" mass="68852" checksum="09C888BB10AF8C7F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAB3</accession>
    <name>A0A8H6FAB3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150480.1">
        <property type="nucleotide sequence ID" value="XM_037293651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAB3"/>
    <dbReference type="GeneID" id="59331138"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="158" mass="18914" checksum="BEC0AE71FC4B089A" modified="2022-01-19" version="1">MQIRPSVEPTDPSKTVSRAQTESTKLSTPLSLSPLIDRSPTTQGRRKKKKKKKREEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEGEEEEEEEEDEEEEEEDEEEEGEEGKGEDEEEERPPPPSNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAQ4</accession>
    <name>A0A8H6FAQ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002413</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150992.1">
        <property type="nucleotide sequence ID" value="XM_037293339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAQ4"/>
    <dbReference type="GeneID" id="59330826"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF0">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01543">
        <property type="entry name" value="ANATRNSFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="35910" checksum="FA62BC869329A1CE" modified="2022-01-19" version="1">MSAKSARPTYDADQISKYLKHISLPEDSKDLFHAPGKLHHCNNALKFLTQLQRHQIATVPFENLSLHYSQDHTISLDPGYLFNKVVDRGHGGYCMENNCFFGTVLRSLGFNVFSAGARVNTGANGTGEDGWEGWSHMVNIVTLSSGQKYMLDVGFGGNGPIQPLLLNPSAAPTQHIAPAEARLVHENIPHNTDPNQKLWQYQHRIDPQSEWQIMYCFTELEFLPKDYEVMNFWTSQSRKSWFTYSVVAVKMILEGEDVVGTLILSGGDVKRRVQGKTEHLRTCKTEKERVQALQDVFGLRLTDEERRGIGGMVTELKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB13</accession>
    <name>A0A8H6FB13_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxamine kinase/Phosphomethylpyrimidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151230.1">
        <property type="nucleotide sequence ID" value="XM_037292691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB13"/>
    <dbReference type="GeneID" id="59330177"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000034">
        <property type="entry name" value="Putative hydroxymethylpyrimidine/ phosphomethylpyrimidine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="317" mass="33505" checksum="7EBA36A34D00F3F1" modified="2022-01-19" version="1">MDRRRILVIAGSDSSGGAGLEADQKVIAAHGCYAMTATTALTAQNTQGVSDIHFVPSDFVIKQIDACMTDIGVDVIKIGMLASARTVDAVANALKKHGEPITVVDPVMVATSGAQLLPNEAVHNLRTNILPLTTILTPNIPEARLLLQNAGLGAPETNSLDDIVNIARVVQSLGPRYVLVKGGHLPLTKDGHISKEEADRHVIVDVLYDGREVAIYQTDYLPSRNTHGTGCSLASAIASGIALSKSPDRTNDIPDAVEMAVRYVEAGIQTSFDLGYGNGPINHFHSLSMRLPPPFPSTLKRSVYGKAQDGGGAAKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB85</accession>
    <name>A0A8H6FB85_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150958.1">
        <property type="nucleotide sequence ID" value="XM_037293305.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330792"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031106">
        <property type="term" value="P:septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000176">
        <property type="entry name" value="Rho guanyl nucleotide exchange factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010481">
        <property type="entry name" value="Cdc24/Scd1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053026">
        <property type="entry name" value="CDC42_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47339">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47339:SF1">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06395">
        <property type="entry name" value="CDC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15411">
        <property type="entry name" value="PH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="213"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="897"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="938"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="897"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="960"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="970"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1038"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1095"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1118"/>
            <end position="1127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1281" mass="143983" checksum="ECB2439D2376B28D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBH3</accession>
    <name>A0A8H6FBH3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anaphase-promoting complex subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001393</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151742.1">
        <property type="nucleotide sequence ID" value="XM_037292323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBH3"/>
    <dbReference type="GeneID" id="59329809"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008401">
        <property type="entry name" value="Apc13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28526">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28526:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05839">
        <property type="entry name" value="Apc13p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11073" checksum="BBE79A98064A48C4" modified="2022-01-19" version="1">MSKDSSHTYIHLNEPRHADLFEEFNRPPHLPHETIYVPPHHQPPNPEDEDDVVPDQHAAFGITRATQAKKESAWKDLGLEGLVGKGPKGGEVGMPKLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBY2</accession>
    <name>A0A8H6FBY2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152101.1">
        <property type="nucleotide sequence ID" value="XM_037291739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBY2"/>
    <dbReference type="GeneID" id="59329221"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF37">
        <property type="entry name" value="ABC1 FAMILY PROTEIN C10F6.14C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="100"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="590" mass="67057" checksum="682B6F2F15C21E0E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCD9</accession>
    <name>A0A8H6FCD9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152084.1">
        <property type="nucleotide sequence ID" value="XM_037291722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCD9"/>
    <dbReference type="GeneID" id="59329204"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="253" mass="28011" checksum="5D8B6560A2DD122A" modified="2022-01-19" version="1">MSDTKALRHRVGFSAYQENTILQRRNPTSPAPQLTLSAPQSNSRSLSTASAMVWMPEEQMWLIAGEMQRDVISSHEPQPAPPAYTPTYIPRNCTRSEPSPTIRSHHDLTPPMTPVQVQLERLLEQQRREPLPQPPSILRDEERFSPLFQEAMNSVPMLDPAELAPPSLFSNPAGKDRPRTAGTPALRSQNSTAIDPRSRSLSVDRQAAHSRNISSSTNSFHSAVEDLNGDANSAPRSWIGLARKIARPASATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD05</accession>
    <name>A0A8H6FD05_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010546</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153032.1">
        <property type="nucleotide sequence ID" value="XM_037301398.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338936"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000614">
        <property type="entry name" value="Serine/threonine protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000206">
        <property type="entry name" value="Serine/threonine-protein kinase Ssp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF152">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="338"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="139"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="474"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="536"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="892"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1011"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1134"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1242"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="186"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="480"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="558"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="928"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1018"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1072"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1134"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1278"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6223972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1302" mass="143287" checksum="C755B9BE1E17A475" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDE9</accession>
    <name>A0A8H6FDE9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010830</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153315.1">
        <property type="nucleotide sequence ID" value="XM_037301681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDE9"/>
    <dbReference type="GeneID" id="59339220"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF2">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="8">
        <location>
            <begin position="99"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="202" mass="22511" checksum="BD52FCE4D240CCD4" modified="2022-01-19" version="1">MAGWTRAPFRRKHSEERAGNEISGQNQASYRLPTTIELEERVTSQVQEGRAEARPGRPRNATESPTTGIDTSHIKRLQHWWSHNVRLNLEHGAPGGDPRDYLALERTFLGWFRTSVALVSLGVVITQLFVLKDVHPRKGRTLGAILSCGGIIVVLLGCVRYLKQQRLLTQGKALSGGWHHQVLIITLLIVLITLFVVVVADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDF8</accession>
    <name>A0A8H6FDF8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153076.1">
        <property type="nucleotide sequence ID" value="XM_037301442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDF8"/>
    <dbReference type="GeneID" id="59338980"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034996252" evidence="1">
        <location>
            <begin position="18"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="173" mass="18990" checksum="AB275975683C5F84" modified="2022-01-19" version="1">MVLQALAALSLAKSVINVVDFAAEVISKGNEYCKSSEGILPENTELQAITDNFARLNKGLRGPRIALKTAAEQPDEKGALQLSKDEKVLQLVAQKCEAIAIGLATALDRLRKALGQIIHLSAILDKAPRSARHEFLEEWQVKYQGEVMRGSGKLPETASDDKYQRIKNLMYTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDM1</accession>
    <name>A0A8H6FDM1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153271.1">
        <property type="nucleotide sequence ID" value="XM_037301637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDM1"/>
    <dbReference type="GeneID" id="59339176"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053221">
        <property type="entry name" value="Burnettramic_acid_biosynth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38887">
        <property type="entry name" value="CHROMOSOME 21, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38887:SF1">
        <property type="entry name" value="RAS MODIFICATION PROTEIN ERF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="414" mass="45440" checksum="C649CD66669DB22E" modified="2022-01-19" version="1">MSSDVAPPYNGSPLKHPNNPCCYEETFSPDRHNGPDCNGPQTDPTDTEVLAEAPQHAQHNPPNEESDHHEQAALDESLSWIANTNRQKHAGVHAPLAVPVVIPQTAPGMQSSFSRAWAPVLAEHDVRPEDFLQFMDHLNVCKAASPPFQVLNLAGTVLGCTFVLPRPCSCLIANQSLPADVTDPNVVRYSPIPFAGLVGLGTSVAAGAGTYATARIRITRYLEQANQEYFAPRGLLARIAKQNVLPQIVGQPENAPLLAPLPRHASKSNGSITPPSLRDRRLQALGSHIARIEFCDLPTPQEEHNMLDKLSAKMTARKTKKQEEKVVKNSMKSQEEESKKKEKLVKEEAKIRQKMEKARFKGSRNEAKMEGKMMEVREKYDEKAGEGGEKEMKAAKKFLFVVVQNAQAAAAADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDZ3</accession>
    <name>A0A8H6FDZ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010049</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153722.1">
        <property type="nucleotide sequence ID" value="XM_037300905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDZ3"/>
    <dbReference type="GeneID" id="59338441"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="42"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="296" mass="33862" checksum="EE8C301D65D5CC3B" modified="2022-01-19" version="1">MAQFAKSTFSHASYAVYRPSYPPSLYNTVLAYHHSPRETCIDLGCGHGVVARYLSQTFSKVVGTDPSDGMIEQALSLTPHEVYPNLTFQNSSAESLKFLKSESVDLVVAGQAAHWFDTKVLFPEMGRILRKSGTLAFWGYKDHVFVDYPKATKILNEYAYSNDERSLGPYWSQPGRSKVENKLRDIEPPVNEFEDVRRVEYEPGTSGPKTGEGTMFLSRRMALEECMNYIRTWSAYSAWQEKFQAKKRRDGGEGDVVDEMFDKMRSAEPDWQKDEAWMEKEVEVEWGSGLLLARRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEC8</accession>
    <name>A0A8H6FEC8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL14m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010156</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153828.1">
        <property type="nucleotide sequence ID" value="XM_037301011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEC8"/>
    <dbReference type="GeneID" id="59338548"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000005">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_uL14_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="133" mass="14390" checksum="3289E706A3FEA653" modified="2022-01-19" version="1">MIQLKTMLNCIDNSGATIVECIANLRMKRHGRIGDRIIVVVQKSRSMGDSGPGNTSAAAANKVRRGDIRHAIIVRTAKKVQRKDGMVVRFDDNACALIGKNGEPLGTRINGIVGAELRSKQQSKLLSLASMHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEJ0</accession>
    <name>A0A8H6FEJ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Autophagy-related E2-like conjugation enzyme ATG3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009470</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154179.1">
        <property type="nucleotide sequence ID" value="XM_037300331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEJ0"/>
    <dbReference type="GeneID" id="59337865"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6225470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="369" mass="40340" checksum="CBBD9D80243D606C" modified="2022-01-19" version="1">MNLIHSTLDTLRDRWAPISHTSTFRTTGQVTPEEFVLAGDYLVYKFPTWSWSDASTPSKRVTYLPPTKQFLITRGVPCHRRLDENFAGDAGQDEAMVQDGFAAGGEGGGGAGGEDDGWLRTGGLAASQEARMRDVRTVDESGNVGEREEEEEIPDMEDEDDDEEAIIRDPKASGTTAPLRTYTVYITYTPYYRTPRLYLSGYSSSSHPLSPHLMMEDIVGDYADKTVTLEDFPFFDNSVKMASIHPCKHASVMKVLLDRADAALKIRLEKVRRGNGGGKGAEGRGKVESGMEGLVDEMNATQIADRRGHSSGVAAAGGGGGDEWEVLQHEGGVSEEDEVAIRVDQYLVVFLKFMASVTPGIEHDFTMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEJ7</accession>
    <name>A0A8H6FEJ7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009592</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154301.1">
        <property type="nucleotide sequence ID" value="XM_037300453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEJ7"/>
    <dbReference type="GeneID" id="59337987"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF49">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04250)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="333" mass="36345" checksum="62A912091B100F5D" modified="2022-01-19" version="1">MGDMDTSKMAPEGGPGNSNSSFDMYTGSGSVEDKSLVRKQDWRIIPLCAVVYLLCFLDRSNIGNARILNITTMSIGGVKIAASLSGVRFLLGVFEAGLYPGVEFYLTFWYRSEERSLRIAFFLPSAALAGAFGGAIAYGVGRMNGINGLSVWQWLVILEGIPACAAGLVVLLILPDYPESARWLTDEEKELARERVRFCGSSADDMAITWVDAKETLTEWRLYFYSLARYICLVISTTFVFSPVPSLLSFLASNTHTTASTDLAVALNVSIGGGAGLILGTWIFPAGEAKNGYKSGNWINAGFMLAISVVCVALRIYYGRANRLKEHKRKFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFF0</accession>
    <name>A0A8H6FFF0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009875</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154582.1">
        <property type="nucleotide sequence ID" value="XM_037300734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFF0"/>
    <dbReference type="GeneID" id="59338270"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="52"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="306"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6225873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="337" mass="37307" checksum="637C9BC98365EC81" modified="2022-01-19" version="1">MASANGTGLAPPPGVTPDFTSPFTLQPYQALTVVACTIVTTVMVAARLYTASFMVFLSIEFSIGRHGGGTHQWDLSYKDVQYNRRYSNYGDIIYSLALFFTKLSIMLLILRIFCSVQRDVGYRLTQFLIAVNGIFYIVYAFIPIFMCFPRSKIWSPEAAGHCLDVNDLYLASAIFNVLSDIAMLSVPIYLIWNLQMSTRRKIGLSAIFLTGGLACIASILRLIYLVPLTRTKDYTYTKIKSILWVDAEIACGLLCSCLVVLPRLYQHLSSVAPNGRNGGLTHGMADQSTRVNAPSRRLRSQWVHLDGMGSPQSPTQAHKVGRSGSDEEALENAVEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG95</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">HO133_008442</name>
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        <name type="scientific">Letharia lupina</name>
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            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037299308.1"/>
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    <dbReference type="GeneID" id="59336838"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="93" mass="9964" checksum="F257FB0BC1FD4B39" modified="2022-01-19" version="1">MDIADSGMFGPKKWYEKTMLLSSGICESMEPFQLVSAGFEMLNIVLLAAGNDTASKTVLAVGALRHCGRIETSAHTLRIVALEPGGNFQGFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGG7</accession>
    <name>A0A8H6FGG7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008834</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037299697.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="term" value="P:regulation of actomyosin contractile ring contraction"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Rho guanyl nucleotide exchange factor, putative"/>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
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        <property type="entry name" value="NUCLEAR FUSION PROTEIN FUS2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1198"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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            <begin position="378"/>
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        </location>
    </feature>
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            <begin position="974"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="1063"/>
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        </location>
    </feature>
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            <begin position="1279"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="1708"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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            <begin position="29"/>
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        </location>
    </feature>
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            <begin position="51"/>
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        </location>
    </feature>
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            <begin position="70"/>
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        <location>
            <begin position="138"/>
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    </feature>
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            <begin position="180"/>
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        </location>
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        <location>
            <begin position="203"/>
            <end position="236"/>
        </location>
    </feature>
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            <end position="448"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1857"/>
            <end position="1872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1977" mass="219221" checksum="745E59147EFED5BA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGG8</accession>
    <name>A0A8H6FGG8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">HO133_008410</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
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            <taxon>Pezizomycotina</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226969.1"/>
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        <property type="nucleotide sequence ID" value="XM_037299276.1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="AB_hydrolase_fold"/>
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        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
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        <property type="entry name" value="Dendritic_Spine_Reg/Scaffold"/>
    </dbReference>
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        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="PANTHER" id="PTHR24166:SF48">
        <property type="entry name" value="PROTEIN VAPYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="4">
        <location>
            <begin position="269"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="871"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="905"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="939"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="973"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1075"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1109"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1143"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1177"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1245"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1279"/>
            <end position="1303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1373" mass="153051" checksum="E77A7F321123F561" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGN6</accession>
    <name>A0A8H6FGN6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3835 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155472.1">
        <property type="nucleotide sequence ID" value="XM_037299471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGN6"/>
    <dbReference type="GeneID" id="59337001"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019212">
        <property type="term" value="F:phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024325">
        <property type="entry name" value="DUF3835"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039553">
        <property type="entry name" value="Prefoldin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052255">
        <property type="entry name" value="RNA_pol_II_subunit5-mediator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT 5-MEDIATING PROTEIN NNX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111:SF0">
        <property type="entry name" value="UNCONVENTIONAL PREFOLDIN RPB5 INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12927">
        <property type="entry name" value="DUF3835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13758">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3835" evidence="2">
        <location>
            <begin position="463"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="545" mass="60085" checksum="5523C55EC6E9B4CA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGT5</accession>
    <name>A0A8H6FGT5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial</fullName>
            <ecNumber evidence="5">1.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Isocitric dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">NAD(+)-specific ICDH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_008742</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-threo-isocitrate + NAD(+) = 2-oxoglutarate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:23632"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.41"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Octamer of two non-identical subunits IDH1 and IDH2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.41" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155607.1">
        <property type="nucleotide sequence ID" value="XM_037299606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGT5"/>
    <dbReference type="GeneID" id="59337137"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000001">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF42">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="13">
        <location>
            <begin position="19"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6227299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="353" mass="38695" checksum="409049577429FD15" modified="2022-01-19" version="1">MATVQSDIFKPTKFGGKYTTTLIPGDGIGAEVAESVKTIFKADNVPIEWEQIDVTGVESGDKHSEELFRESIASLKRNKLGLKGILHTPVERSGHQSFNVALRQELDIYASIVLIKNIPGYKTRHDNVDLCIIRENTEGEYSGLEHQSVSGVVESLKIITRAKSERIAKFAFSFALANHRQKVTCIHKANIMKLADGLFRDTFHQVAQDYPTLTVNDMIVDNASMQAVSRPQQFDVMVMPNLYGGILSNVGAALVGGPGIVPGCNMGRDVAVFEPGCRHVGLDIKGKDQANPTAMLLSGSMLLRHLGLDEHANRISKAVYDVIKEGKTRTRDMGGNSTTHDFTRSVLDQMETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHF5</accession>
    <name>A0A8H6FHF5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007991</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156195.1">
        <property type="nucleotide sequence ID" value="XM_037298859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHF5"/>
    <dbReference type="GeneID" id="59336388"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052374">
        <property type="entry name" value="SERAC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182:SF2">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="8">
        <location>
            <begin position="20"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="306" mass="34106" checksum="0AD11BB3E7C18710" modified="2022-01-19" version="1">MTPITGLTELYRGDDPVVDIVAVHGLNGDPFRTWTTGGSEKMWLKDLDLLPSNLKNSRILTYGYDANVTAMFGKTSSDRILQHAHTLIAQLVADRELEDATQRPIIFVCHSLGGIVVKRAIAYSASRTSKSIQHVHSIYVSTFGILFLGTPHDGGNKAKLASTSRRMIGALVPSKILDTEGQLLDAVQEGSEVLQNITDMFVPLMKDFHIFFFWEQEKTNMGVTQDYVVEESSAAPILDSTERAGLPGDHRNMCKFESRTSPGYRLVVAALLRYSKEAPEVVSRRWKYAVDMLNSKRSQEADELLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHH5</accession>
    <name>A0A8H6FHH5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trehalase</fullName>
            <ecNumber evidence="4">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Alpha-trehalose glucohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008051</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156255.1">
        <property type="nucleotide sequence ID" value="XM_037298919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHH5"/>
    <dbReference type="GeneID" id="59336448"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018232">
        <property type="entry name" value="Glyco_hydro_37_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403:SF6">
        <property type="entry name" value="CYTOSOLIC NEUTRAL TREHALASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00744">
        <property type="entry name" value="GLHYDRLASE37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00928">
        <property type="entry name" value="TREHALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="646" mass="73090" checksum="3775FA08701D2161" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHK0</accession>
    <name>A0A8H6FHK0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156330.1">
        <property type="nucleotide sequence ID" value="XM_037298994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHK0"/>
    <dbReference type="GeneID" id="59336523"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37327">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37327:SF1">
        <property type="entry name" value="MICROTUBULE INTERACTING AND TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="2">
        <location>
            <begin position="170"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="521"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="615"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="827"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="872"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1050"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="681"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="759"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="774"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="827"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1179" mass="127931" checksum="80C740E21B5370CC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHP3</accession>
    <name>A0A8H6FHP3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156148.1">
        <property type="nucleotide sequence ID" value="XM_037298812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHP3"/>
    <dbReference type="GeneID" id="59336341"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="661"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="764"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1042"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="681"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="722"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="787"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="803"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="838"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="878"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="945"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1062"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1145" mass="126605" checksum="0B7AC51902A1F3A6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIH1</accession>
    <name>A0A8H6FIH1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asaB hydroxylase/desaturase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156744.1">
        <property type="nucleotide sequence ID" value="XM_037298109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIH1"/>
    <dbReference type="GeneID" id="59335616"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044053">
        <property type="entry name" value="AsaB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041278">
        <property type="entry name" value="CmcJ_NvfI_EfuI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34598">
        <property type="entry name" value="BLL6449 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34598:SF3">
        <property type="entry name" value="OXIDOREDUCTASE AN1597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="344" mass="39582" checksum="6354F8D50A484C7B" modified="2022-01-19" version="1">MFGVLRGAFTRKGGFYQCSRQAANISRSHWISTTNNARLWYLKRLDLYQTEKPYHINLPANALGRHAQSNEVSQEYAGIRIKSLRGFENDFNLDKNGFQIFEDIDCGDDADWSTHSASAAPTSDVYADPDIVRKKYYPAIERLLKKKLGAQMAKAFTHDVRRREPQFPALPRGTGTSPQPVQGVHVDLTPRWAEEKLHMLFPAEQTWGIMQHRWQVLNVWRPLFGPLQDWPLGLCDYSSINPQRDLVPSDNIYTHIVTETYNVYHDNAHRWYFLGDMKPNELLVFKSFDTMAAEGNARVCPHAAFEHPETPANARLRESIECLVYVIYPERRSTASRTDIDLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJY0</accession>
    <name>A0A8H6FJY0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157185.1">
        <property type="nucleotide sequence ID" value="XM_037295184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJY0"/>
    <dbReference type="GeneID" id="59332674"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="206" mass="22455" checksum="E24DD6B35ED8575E" modified="2022-01-19" version="1">MASASSIQLPPGVSPNHLFGPLKELIDELRTFTHDTTENGQSDDLGVRTITAGSNSDALVPLFFRCTWPAGARSRHKLIWMKPSVPNAALGAAVNDAFRVIEAGGVVEKTVAIWGVNNPFADSAARGITVRERVRRAVGLDDPPTQQQIIKMMEGTMNGIMQVVVEPERDARKCVRLGWQEINRIIRQAEAEGEQRARSSLNNSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK06</accession>
    <name>A0A8H6FK06_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157174.1">
        <property type="nucleotide sequence ID" value="XM_037295173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK06"/>
    <dbReference type="GeneID" id="59332663"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="459" mass="52274" checksum="E3D607F15F2E5F52" modified="2022-01-19" version="1">MPVRAPLDCLKVGAAFMGMPRLANLTITELSDSQEFVDEFCHLGDGILALSSSLRSLNISISNCNRAEGWEKDEAFVEPGDVAFFFKKFFLEPSCDRIEALVKARYNDPREPPDVNILRSSKGSLNLERICLKNIGLPWWSFQTVFNPKTVKELNLPTCRVAPNVWDDLGEHAQVHKVANINYEMLSGPFMSFLSTQHNLHFLSFARPPDIYNVANIRSNLLDTRVDVASFSVTKAAPHLGNGTEWGRACARNAWLLQSKESWIQHEHLIQSPSSWSYFEDLIRSLNSYFQCQYPRKSDFVKALSTKTSLKHLVLPADMFDITPKFMACLAIELPALESIEWGFDYACPELRACFIKNFLPSHPRLKKITFLSLNRPHPLSHFDANEFHSAFANGSCDLAPAVLKYVRYRDLNGKSYKGKFTKSDVYYHRKTMANGTWWLKIPARKADSMFEDEITPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK98</accession>
    <name>A0A8H6FK98_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sorting nexin-4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Autophagy-related protein 24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004463</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157381.1">
        <property type="nucleotide sequence ID" value="XM_037295380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK98"/>
    <dbReference type="GeneID" id="59332871"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07628">
        <property type="entry name" value="BAR_Atg24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06863">
        <property type="entry name" value="PX_Atg24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000035">
        <property type="entry name" value="Sorting nexin-4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000042">
        <property type="entry name" value="Vacuolar targeting protein Atg24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949:SF2">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="67"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6230124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="467" mass="52882" checksum="4F499D2199A0FD7C" modified="2022-01-19" version="1">MDNDFDSVSWQNDPDSDSSRPTTAGTSRPDETAYGAKSTGKRKASHSSAQAGADADPVDLAGIGDGRLDCTVDTPLKENDGTKDSYVSYMVTTKTDFASFQKSTVKVRRRFTDFVFLYKMLCREYPQCAVPPLPDKHKMEYVRGDRFGPDFTHRRAHSLHRFLKRLVLHPTLRRAALLMIFLESTDWNSTMRTHPKRSISVSESGTSVFDNFADTFVNAFSKVHKPDKRFIEVREKADKLDEDLGHVSRIVARLARREGDLESDYTDLSIQFMKLGALEPGVEGPVHAFAQGVEETAKGITALKEHTDQSYLGSLRDMEAYTSSLKSLLKYREQKQLDYEGLTEYLTKASYDRDTLASSGHTSQGPTGFIRSKIEDVRGVDHEVSRRDRVRKLEMQIEKLVREVEDARRTTEAFDGEVVREVGDFERIKAGEFRDCLGALADKHVEFYSGVVETWESYIREMEAEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKK3</accession>
    <name>A0A8H6FKK3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">T6SS Phospholipase effector Tle1-like catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157500.1">
        <property type="nucleotide sequence ID" value="XM_037295499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKK3"/>
    <dbReference type="GeneID" id="59332990"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018712">
        <property type="entry name" value="Tle1-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840:SF2">
        <property type="entry name" value="TLE1 PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09994">
        <property type="entry name" value="T6SS_Tle1-like_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T6SS Phospholipase effector Tle1-like catalytic" evidence="2">
        <location>
            <begin position="4"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="343" mass="38132" checksum="8EA34889CA0787DB" modified="2022-01-19" version="1">MQRSKFPYTARSSARVIRHAVSIDERRAKFRSDLISGAKTPKTPEREEHHRETHGQKTGARENLMSNGSNASQLGAGRFRRRSQFSSTVELNKAMSPRLSAVEEGYLDPRGSSHGRLPSTGPAISRNAASDGVSIITSTSIDSYQPGQHPVLKSKIRKARIWMETFVISQASCAKEAMLTPRLCVGAAGMEPETSQESEFQQTLFRAATKGVLHDCLEFSNGLRVESVLSWKMMEWFPFRRMDLRPDGSWKAISIPLPRAEVRDMPENAWIHHSAIRRMEADGRYRPGNLIIGGGGRGIKRGPKALGIGAWEILKGKDDPVGMVYVRKGPSIEKQIDKATHET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKL3</accession>
    <name>A0A8H6FKL3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">6-phosphogluconate dehydrogenase C-terminal domain-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004601</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157518.1">
        <property type="nucleotide sequence ID" value="XM_037295517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKL3"/>
    <dbReference type="GeneID" id="59333008"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015814">
        <property type="entry name" value="Pgluconate_DH_NAD-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09130">
        <property type="entry name" value="DUF1932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="2">
        <location>
            <begin position="8"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphogluconate dehydrogenase NAD-binding putative C-terminal" evidence="3">
        <location>
            <begin position="210"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="34503" checksum="B1D63F24FB975042" modified="2022-01-19" version="1">MPPSLATVGVASIGEMGVGIARLLSAQNYRVLTNISDATSESTRQRVKSASIELVDTDQELVAQADYILSIVPPRDAISTAKRFTEAAAGHVRDPEQEPLFYIDLNAISPNGARTLELLLKDYPMMRFLDGGIIGGAPKYKPDGSWTKPSIVVSGPHSLNDAPISGAHLAKVLNIKHIADKIGPASGLKMCFASTTKGLTAIAIQSFTTAHTLGVLDDLQAHLKDYSPKTGELAAQGLVGMPPKAYRWVDEMKEIAETFRTEGGFEKNLFDGVSEVFRFVADETNLGKEKTEDRRVGKTPEDVARLVGQGLERKKEKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKU9</accession>
    <name>A0A8H6FKU9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157646.1">
        <property type="nucleotide sequence ID" value="XM_037295645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKU9"/>
    <dbReference type="GeneID" id="59333137"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="30032" checksum="FDF93882604D337E" modified="2022-01-19" version="1">MIPPKGGCYNLLWAAAGDKEAIINGELVGGSVSGFGEDESLAELEDEELPEFGEESLPEFEEESLSELEEESLPELDDESLPELDDVPLPEDDDDSLPELDDDPSPDEEEESLPELDDEPLPELADELLAALDEDPPPEDDDEAPPELDDALPELADELDAALDEPSKFPNADPYDLSSNAEQSGNNCANPTTTPGPTAPKNSSPVLTGCASVWSQYPASVHWSLIFTTTLVPTESPLEGYQSLWGVMCQHWPQPLPTPETILAAPQSTVSSPRLSQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKW8</accession>
    <name>A0A8H6FKW8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157681.1">
        <property type="nucleotide sequence ID" value="XM_037295680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKW8"/>
    <dbReference type="GeneID" id="59333172"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="565" mass="61430" checksum="DA92DDF07F87A2AA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKZ1</accession>
    <name>A0A8H6FKZ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LIM-domain binding protein-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157675.1">
        <property type="nucleotide sequence ID" value="XM_037295674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKZ1"/>
    <dbReference type="GeneID" id="59333166"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10378">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="303"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="485"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="723"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="901"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="247"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="827"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="723"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="744"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="901"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="949"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1017" mass="112686" checksum="1921A7419FAD8E5A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FLM1</accession>
    <name>A0A8H6FLM1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7924 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157815.1">
        <property type="nucleotide sequence ID" value="XM_037290941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FLM1"/>
    <dbReference type="GeneID" id="59328420"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057684">
        <property type="entry name" value="DUF7924"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25545">
        <property type="entry name" value="DUF7924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7924" evidence="1">
        <location>
            <begin position="146"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="363" mass="41505" checksum="ABBA44343F23BED3" modified="2022-01-19" version="1">MNVSTVRANLKSRGFYYDDEDALARGGQAIKDKAREIITSQRNSPTSNDNAPLLKAAIRKYCGSTERTLVFNVWCILRQQKLRDITEKPLSPQGEDNALAWVKRAWSMDFLDCVFEADLNKDMIPDTSHTGSKTTDIMFKDLPRIEQPRPDVTYGLWKADIDSPCRGILDTYHCELAKDVELPWFLVEVKTLNAVVGEAENQCIRGGAVLVNGHHQWNRVADGVKHSLDLSKKEKEEEHDRLIAKREKAGTAYDKLQSTADSATLAFSLAFSPSTATMHVHWREEWENGVEHWHAHTIQTYAMRTEHRDYEQLGMHMANIMDWGCRTRRMEIEKQAKVIAKRKYPGIDENEVCFAPNKRQKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY35</accession>
    <name>A0A8H6KY35_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycoside hydrolase family 71 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156602.1">
        <property type="nucleotide sequence ID" value="XM_037297967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY35"/>
    <dbReference type="GeneID" id="59335472"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051118">
        <property type="term" value="F:glucan endo-1,3-alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11577">
        <property type="entry name" value="GH71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005197">
        <property type="entry name" value="Glyco_hydro_71"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03659">
        <property type="entry name" value="Glyco_hydro_71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="45359" checksum="CDDA0A21F7953F34" modified="2022-01-19" version="1">MVFAHYMIQFAPPGLNGNPNPDYSTDINMAKAAGFDAFAVDYTDNAALFATNLDILYHTASTLGFKLFLTIDTTTMYDVAQVVNITSFYANSPAQLKDGAGNIFLSSFEVNPPGWNWQTDVIDQLNIQNIGVNFLPGSLGQDGAYEASLPDRGTGLFTWIHPALSASEEAAVDQTFADARDSSGKPWMAGVAPWFFKRLASDTNWLNAQDSNIYIDRWVELLKLKPDYIEVISWNDFGESHYIGPADSTPEAELMLPSKADYYGNLDHSAFLKMSTFFINTYKAGETNVTVTADEEDVFFFYRPQPVNNIPTNDTYLDNAWPLPENASSIADNVYVVPFLSEPATIYLSSGGYSTSMDAPSFLSKSAITFNSPAMMGQQILTASRPINGQTLNKTGTVDITSGGSRYQGNVVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYC6</accession>
    <name>A0A8H6KYC6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156619.1">
        <property type="nucleotide sequence ID" value="XM_037297984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYC6"/>
    <dbReference type="GeneID" id="59335490"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF73">
        <property type="entry name" value="HET DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G01020)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="2">
        <location>
            <begin position="132"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="794"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="802"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="835" mass="95196" checksum="07878AC5E826A124" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYV4</accession>
    <name>A0A8H6KYV4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007510</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157036.1">
        <property type="nucleotide sequence ID" value="XM_037298401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYV4"/>
    <dbReference type="GeneID" id="59335909"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040085">
        <property type="entry name" value="MJ0674-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43075">
        <property type="entry name" value="FORMATE LYASE ACTIVATING ENZYME, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G15630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43075:SF1">
        <property type="entry name" value="FORMATE LYASE ACTIVATING ENZYME, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G15630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="44"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="234"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6229394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="310" mass="34101" checksum="2E6562CEA8DF3FAA" modified="2022-01-19" version="1">MTTSPDISFYRVCLIGAETAKVNVIAPHFGEEPCIQGHNGSGSVFFSGCNLRCVFCQNHDIAHQRNGFDATPEELAEWYMKLQNVGKVHNINLITPEHVVPQVVLSILRAKDLGLRLPIVYNTSAFDSPESLELLDGLVDIYLPDFKVWTKASSKRLLKAEDYASAAMESIKAMHAQVGDLCFTTDGIAKKGLLLRHLVMPGKEEEGQEIMRWLGDNVSRDIFVNIMEQYHPDAHVGKSSRSSPAPSSVGSSEELPTSEPGGGPSSITSARYSEINRTVTGQEISSVRRAAENAGIWRFCDPVRHDGFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C4F9</accession>
    <name>A0A8H6C4F9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006808</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146905.1">
        <property type="nucleotide sequence ID" value="XM_037297704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C4F9"/>
    <dbReference type="GeneID" id="59335208"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="229" mass="25570" checksum="790DBC7153EFC1FA" modified="2022-01-19" version="1">MFHQRASIVRTAVEVAEAWDEDLVAALTDIGGWKRKFDFGQWHTPNDASSRISGKGEKRVATVTLKYSPVDDLFNTSLKVSNLTQKRRIHQPDTISRQAGYPAYTSYAKAIQAQQAPRTPKDPLRTPLPKAQTPESFRRSTSSRRRGEALYRLEVFRPGYLRTSSSQMPGDVISTSSPKNDARDRVGSVGIGDSICTCEQKKKGKANRTSSLPLDGRLVLRYQGRCALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6C6</accession>
    <name>A0A8H6C6C6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Galactose oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006824</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146921.1">
        <property type="nucleotide sequence ID" value="XM_037297720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6C6"/>
    <dbReference type="GeneID" id="59335224"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019760">
        <property type="term" value="P:glucosinolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47435">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47435:SF10">
        <property type="entry name" value="TIP ELONGATION ABERRANT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="368"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="51245" checksum="28C76C5C3D3777A4" modified="2022-01-19" version="1">MDPATVTGLVVAEQVLSTTVEGGAIATYGLAQSTQPLSATFKRVSSPAFLPRSNHSLTVLAGRAYIFGGEDESGKLAGDEVHIVTLPLKKKGNHDGEVDYKCVPSLGEGEDGKVPGPRAGHMAVAIGERIYLFGGKGTDGQAIEEMGRVWVFDTNTLGWSYIDPASDTATTSVPPARYHHGAASSEHPLPQTNASSKASYSEQITSTIAKLPSLMAKASSPQEPHGSLIICSGLSAPSSPLNDTWLFNITTRTWSALPSTPSTSPSPPSIALTYNRLYLITSTSDFGSEIHYLPITKETYSDARGEGEVGLTSSKNEWTTLPFPTNPFAPGPRPRKGAGLMPVTTGNGREYLLYFLGEKAATTQPAEPAEKEEEKTDKDEPTFWSDSYSYQVPAIPVTAAGIKDATRQAMGIATGEDTWAEVKVEAGIESKEKDSEGKAHPGPRGWFASCIVPSQPGSVVLWGGVNGKGETEGDGWLVDIGTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6H3</accession>
    <name>A0A8H6C6H3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome biogenesis protein SLX9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006864</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLX9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146961.1">
        <property type="nucleotide sequence ID" value="XM_037297760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6H3"/>
    <dbReference type="GeneID" id="59335264"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003739">
        <property type="entry name" value="Lys_aminomutase/Glu_NH3_mut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028160">
        <property type="entry name" value="Slx9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538:SF0">
        <property type="entry name" value="L-LYSINE 2,3-AMINOMUTASE AQ_1632-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538">
        <property type="entry name" value="LYSINE 2,3-AMINOMUTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15341">
        <property type="entry name" value="SLX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6217526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="376" mass="41304" checksum="4CDB1AF825CF23BF" modified="2022-01-19" version="1">MSVRLTPHVLSVIDWRKPMEDPARKQFIPLGSTSQEDHPKLRLDSLSERDDSPTRFLPIQKKWGKMFEYIERTPSLHDVMIPGGDTSSLEPAQLDNIDHRLLSFPHVQRIRLASKGLAVCPSLILDGIDSWAKRVIQPLDPGRNIGKSVALHTQFNHPNEVTWITSSAAQKFFEIGVTVRNQTAPRPPTAPSKNPHKPAKAPSRKREKRISKHATLLSRITKPTAATSKVTKRRRPSKKLVTGLDTLADALPRATDGDGEEAWEGIVAEGGGEDGARKIKHRTLKSRPGAGKKKEKLVAMERERFARNLAQMAAGRVSVPGPADADGGVMIVDGREEGEGTKKGRGSADRWAAIRGFISQTMERRVGGEVGVGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7J8</accession>
    <name>A0A8H6C7J8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005777</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147863.1">
        <property type="nucleotide sequence ID" value="XM_037296682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7J8"/>
    <dbReference type="GeneID" id="59334182"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06097">
        <property type="entry name" value="Aspergillopepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000092">
        <property type="entry name" value="Aspartic endopeptidase (AP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034163">
        <property type="entry name" value="Aspergillopepsin-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF1">
        <property type="entry name" value="ASPARTYL PROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="93"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="53"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="435" mass="46505" checksum="604D994F9E718FC0" modified="2022-01-19" version="1">MATKLQRVKLIQNKNYQRSGPKSYVSLLHKYKFTPTLDGPYTVINKAHHQGKHGEHKAVGGKTHMQKVLQKKAPTSDQTGDVPAEDSQNDSEYLCPVTIGTPGKTFTLDFDTGSADLWLWSTELPAATTSGTSHTIFDPTQSSTFKNSSGSSWKISYGDNSSASGTVGTDVVNVGGLIIQNQAIELAQTLSTQFQQDSSDGLLGLAWGSINTVTPNPVQTPVENMISQNDIPKTSELFTANLGSVKDANDPDKGESFYTFGYIDQDALGGQTPYYTPVDNSQGFWMFDSTSAVVAGKTITRSGNTAIADTGTTLALVDDTTCEAIYKAIPGGKYDSTQQGYIYPSNTALNKLPVVSFAVGGQQFALQKEDLGFADAGNGMTYGGIQSRGTMTFDILGDVFLKSIYAIFDQGNTRFGAVSRSEPTQNTTIPAASSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7V3</accession>
    <name>A0A8H6C7V3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148017.1">
        <property type="nucleotide sequence ID" value="XM_037296836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7V3"/>
    <dbReference type="GeneID" id="59334338"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990879">
        <property type="term" value="C:CST complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024222">
        <property type="entry name" value="Ten1_fungal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12658">
        <property type="entry name" value="Ten1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="159" mass="17603" checksum="A936E1E1B72C9C12" modified="2022-01-19" version="1">MNNGPLPTRIVFLSDLPTQAHGTKVRFLGCVTRYTFSTGVLELQHAYHLPPNQPTVALVDVNVILEGLEREDTQVGAWVNVLGYVEEVLEEGKRERGQGKGKTGLAKRKGDRIREGPRAGRVRVRAVVLWSAGGVKIGEYERTLEERLELDKGSREGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C891</accession>
    <name>A0A8H6C891_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005818</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147904.1">
        <property type="nucleotide sequence ID" value="XM_037296723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C891"/>
    <dbReference type="GeneID" id="59334223"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF130">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="90"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="479" mass="51401" checksum="772966091B213DBA" modified="2022-01-19" version="1">MPKDAVDSTAADSEKDVHNGERESSSRSDGEGQCSVDSPEIDPSPKEAPAMGVKSIDEPGPGGVLAKVLSRTTSRISIDPGPPPDGGLAAWTQVLMGHLIVFNTWGYINSFGVFQTYYVATLGHPPSDISWVGSVQIFLLFFVGTFSGRATDYGLFRITFLIGSILQLIGVFMTSLSTKYWQLFLAQGICTGLGNGLIFCPALSLLSTYFSRKRSLAIGIAAAGSATGGVVFPVIVRQLLPKLGFPWTVRVVGFVMLAVQAVTLTFTKPRLPPRKTGPIVEWGAFKELPYALFAIAMFLNFWGLYFAFYYIGSFGRDILGISQAEAISDLLILNGVGLFGRLIPAHLADRYFGPLNTIIPFAFISGLLLYCWSAVRSKGGLVGFAVIYGLFASGIQSLFPATLAGLTSDLKKAGVRMGMVFSVVSFACLTGPPLAGALIQRRDGSYLYAQMFAGSVMIGGCMTLMACRVSKNGWKRARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8C9</accession>
    <name>A0A8H6C8C9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-conjugating enzyme E2 2</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">E2 ubiquitin-conjugating enzyme 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquitin carrier protein UBC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ubiquitin-protein ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin-protein ligase UBC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_005393</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148285.1">
        <property type="nucleotide sequence ID" value="XM_037296303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8C9"/>
    <dbReference type="GeneID" id="59333799"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23796">
        <property type="entry name" value="UBCc_UBE2G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000008">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0882">Thioester bond</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="17">
        <location>
            <begin position="5"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="15">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6218850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="166" mass="18648" checksum="46D378ED9859BD14" modified="2022-01-19" version="1">MASSIASRRLFHEYKLLTSSPPDGITAGPVSEDDIFLWEALIQGPDGTPFEGGIFPAELKFPKDYPLNPPTMRFLGEVWHPNVYPSGLVCISILHAPGDDPHHYEKASERWSPIQSVEKILISVMSMLAEPNDESPANIEAAKMWRERRSEFEKTVREGVRRSLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8E0</accession>
    <name>A0A8H6C8E0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005893</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147979.1">
        <property type="nucleotide sequence ID" value="XM_037296798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8E0"/>
    <dbReference type="GeneID" id="59334298"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057688">
        <property type="entry name" value="DUF7928"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35408">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35408:SF3">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25550">
        <property type="entry name" value="DUF7928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="709"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="748"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="788"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="844"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="874"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7928" evidence="4">
        <location>
            <begin position="68"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="3">
        <location>
            <begin position="520"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="900" mass="102057" checksum="1BC85033D7F323FC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8N3</accession>
    <name>A0A8H6C8N3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vacuolar protein sorting-associated protein 54 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005572</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148463.1">
        <property type="nucleotide sequence ID" value="XM_037296481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8N3"/>
    <dbReference type="GeneID" id="59333978"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039745">
        <property type="entry name" value="Vps54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012501">
        <property type="entry name" value="Vps54_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07928">
        <property type="entry name" value="Vps54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 C-terminal" evidence="8">
        <location>
            <begin position="830"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="145"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="547"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="799"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1077"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="804"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1092"/>
            <end position="1105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1105" mass="120425" checksum="B74C27DA7B1D6090" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C963</accession>
    <name>A0A8H6C963_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MTHFR SAM-binding regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005449</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148341.1">
        <property type="nucleotide sequence ID" value="XM_037296359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C963"/>
    <dbReference type="GeneID" id="59333855"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000002">
        <property type="entry name" value="Methylenetetrahydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF1">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21895">
        <property type="entry name" value="MTHFR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="10">
        <location>
            <begin position="379"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="353"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="647" mass="71822" checksum="EADC8B79767424CC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA49</accession>
    <name>A0A8H6CA49_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HOOK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003588</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149198.1">
        <property type="nucleotide sequence ID" value="XM_037294510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA49"/>
    <dbReference type="GeneID" id="59331999"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22211">
        <property type="entry name" value="HkD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF40">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116907">
        <property type="entry name" value="Hook domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="320"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="363"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="549"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="684"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="837" mass="95669" checksum="36FA0DDC4651A2AA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAA3</accession>
    <name>A0A8H6CAA3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_003725</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149335.1">
        <property type="nucleotide sequence ID" value="XM_037294647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAA3"/>
    <dbReference type="GeneID" id="59332136"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001409">
        <property type="term" value="F:guanine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000010">
        <property type="entry name" value="Probable YHM1 (Mitochondrial carrier)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053042">
        <property type="entry name" value="Mito_GTP/GDP_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46974">
        <property type="entry name" value="MITOCHONDRIAL GTP/GDP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46974:SF1">
        <property type="entry name" value="MITOCHONDRIAL GTP_GDP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="219"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="33219" checksum="206BA3BED181C23C" modified="2022-01-19" version="1">MAPQVVETVASSAAKEFKKESAAARLLGSGSAGIAELAIFHPVDTIAKRLMSNQGKVASAARLNEVVFRDTAHATVGKKFTSLFPGLGYAAGYKVLQRIYKYGGQPFARDYLALHHGDQFDRTFGKGNGKAIMHATAGSFIGIGEIVLLPLDVLKIKRQTNPEAFRGRGVFRIVADEGMGLYRGAGWTAARNAPGSFALFGGSAAAKQYLYKLDDYNSAGFMQNFVASIAGATASLLVSAPLDVIKTRIQNRNFENPESGFKIVGSMMKNEGFTSFFKGLTPKLLMTGPKLVFSFWLAQTLIPAFNTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAW7</accession>
    <name>A0A8H6CAW7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003808</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149418.1">
        <property type="nucleotide sequence ID" value="XM_037294730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAW7"/>
    <dbReference type="GeneID" id="59332219"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF7">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="386" mass="44825" checksum="2532939BF674A827" modified="2022-01-19" version="1">MTKCVLHGIASKWPALSFIDRGPTSYEPVVPAITDHMHPPAMSSISVYGYDSEMLAVSDSLYFHHHSREPPKAEQLQASRPKGCPFLKLPYELRAHIYNYVLPSTTDHPDRGIVWLRATAAIWATNRLVYKECIRLIYGNPTFLIDIKFDKAEFLYQWVLPQRSLVPKRIFNFPDPIVARNRPLMRNFYVRVHQVDSYTGMIKYNYSNPEVLARGLRCQVNILCAFLKEMHEIRELRISYHGGNEESHAVLPLVMDPFWQLKRTKKVTVEDPGLVNEALRTRLQEHLTDAYTKNSLMRLPLELREHVYRHALPHTLSTGTGDKKVITWYPGDISILGTCRQVNVEATRVLYATNEFEFSWMLKYPSEHDWQEEVSNCSDYLERSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB05</accession>
    <name>A0A8H6CB05_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RuvB-like helicase</fullName>
            <ecNumber evidence="15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_003756</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA helicase participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RuvB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149366.1">
        <property type="nucleotide sequence ID" value="XM_037294678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB05"/>
    <dbReference type="GeneID" id="59332167"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002221">
        <property type="entry name" value="RuvB-like 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000002">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="68"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6219931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="468" mass="51541" checksum="B4E83415412EE98E" modified="2022-01-19" version="1">MAAAITTVSESRDLRGLNLVAAHSHIRGLGVDEDTLTPRASSQGLVGQERARKAAAVILQMVKEGKIAGRAVLIAGPPSTGKTAIAMGMAQSLGSDTPFTMLASSEIFSLEMSKTEALTQAFRKSIGVRIKEESEMIEGEVVEIQIDRSVTGGNKQGKLTIKTTDMETIYDMGTKMIDSMSKERVMAGDVISIDKSSGKITKLGRSYTRSRDYDAMGADTKFVQCPDGELQKRKEVIHTVSLHEIDVINSRTQGFLALFSGDTGEIRSEVRDQINTKVGEWKEEGKAEIVPGVLFIDEVHMLDIECFSYINRALEDELAPIVIMASNRGNTRIRGTSYRSPHGLPLDFLDRVVIVSTQAYGEDEIRQILSIRAQEEEVEVTANALELLTKIGKERGLRYASNLITTSTLLSQKRKADKIDQQDVERSYTLFYDPTRSVQFVSDFEKRFISDEGAVSFAITNGDAMEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB32</accession>
    <name>A0A8H6CB32_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF962 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149615.1">
        <property type="nucleotide sequence ID" value="XM_037294234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB32"/>
    <dbReference type="GeneID" id="59331722"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046521">
        <property type="term" value="P:sphingoid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009305">
        <property type="entry name" value="Mpo1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026:SF9">
        <property type="entry name" value="2-HYDROXY-PALMITIC ACID DIOXYGENASE MPO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026">
        <property type="entry name" value="DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06127">
        <property type="entry name" value="Mpo1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="21673" checksum="D329BA747024CB37" modified="2022-01-19" version="1">MSLNLEKQLLFYGSYHHTTANKAIHIVCVPMILMTSFLLGTNTPSLVPMPRWLVIPNLPPNLGTIATLIYAILYILMEPVAGGLLAPLLLAGTAYSDYLTTTYGATANYWAVGIFVSSWVTQFLGHGVFEGRAPALLDNLVQALFLAPFFVWMEVLFSIGYRPELKSRVDSAVEKEVERYRKSQGQSNGSATNGKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB90</accession>
    <name>A0A8H6CB90_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1989 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149815.1">
        <property type="nucleotide sequence ID" value="XM_037294434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB90"/>
    <dbReference type="GeneID" id="59331923"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018959">
        <property type="entry name" value="DUF1989"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527:SF0">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09347">
        <property type="entry name" value="DUF1989"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1989" evidence="1">
        <location>
            <begin position="18"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="22825" checksum="3A45CC2C2D434373" modified="2022-01-19" version="1">MATSDRSEQLAPGQTVMIPSRSGRAFTMKKGQRLTVIDPRGEQVSDLLAFNTHDLDEVISSGRTLDYASRIYLTKGDPLYSNRSNVMLRIIEDSVGRHDFLLTPCSKDTFRIIYGEKEPHHGCFGNLSTALMPYGILSDRIPVAFNCFMNVAIDGTTGQLCVDPPKSKAGDHITFEAEMDLTMALTACSALQSNGGSFKPIHYRIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBC0</accession>
    <name>A0A8H6CBC0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MYND-type zinc finger protein samB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149828.1">
        <property type="nucleotide sequence ID" value="XM_037294447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBC0"/>
    <dbReference type="GeneID" id="59331936"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="30891" checksum="13216C0D8168C452" modified="2022-01-19" version="1">MNEAAQAAQQSHTYNTADWYDHHDIYKAKQEQAKDRENRVKEASSGVLCVVCNTFKALPCVYCKNAPSPSGDPVPFVYYCSNTCATSDWPNHEAYCLASKNRRALFEVAEVLQEKYYTACAMDLLEVCQTGLNCSQMVRYDRQSLQRRIDAISENREQDKAFLGCFGGNNPMLFRRREVTKHFEGLDIEIHEMTICMEDRTRRFSFEEQAISIMDQHEVLQITMNNSEKYVLDIAGAQFGLYAPIVPFQQYVDSHVREIKGYKDFVYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC35</accession>
    <name>A0A8H6CC35_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150095.1">
        <property type="nucleotide sequence ID" value="XM_037294017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC35"/>
    <dbReference type="GeneID" id="59331505"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="389" mass="44351" checksum="F967C144D8EAB3E9" modified="2022-01-19" version="1">MNAHELVPKDAICTKKGEIQEGSECEEDSALYHHNYRKELLAQAAKAVGATYGNVLRNYPYAPLTLKHTDYQPSCGSAPFDVTAVHEDQLSWGKDIKYRPVDLLSFNPPPAENMSKKAIGNLKYDGKLLLDFRGQPIRDFPSLPLVISSMVEGWRAEAWMRSGQMSVMDIVARIPVKWTTDPAGQQTPEPIYNSGTVRERARRFRNDAGLISWTTGAKDSKIENFMDELRSPAEKANNQTIGRDLTDLEKASMRSLNIGTRPERAQVRQDPEAKQKYLDKVQKKADLTFDFDCRAERPETPEDIQEVQNALQQTYSDFAFYTNVPLEMPNTQATYLDQWNCMQVQLNRIWANDRTKKTEPPQLFALGKWTVSFDNWVSAPQAYLTYVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCW0</accession>
    <name>A0A8H6CCW0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150423.1">
        <property type="nucleotide sequence ID" value="XM_037293594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCW0"/>
    <dbReference type="GeneID" id="59331081"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034470805" evidence="1">
        <location>
            <begin position="20"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="159" mass="15945" checksum="A497DB1EC7682E32" modified="2022-01-19" version="1">MMRLFTLVSLAATALTASANPALFPPLLPSFFPAGPTVGSIISTALAAAPIPSAVAPVVASAVAAVPATPNNASADSIPGLDIPPTELFDNILAAIAILDVVLTLLNSSGLADTQSVKIRDLDLPTSGLTDLLEVQGSLGYTITTVTDLAVPLLSTSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD82</accession>
    <name>A0A8H6CD82_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_002253</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:21564"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.3.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150833.1">
        <property type="nucleotide sequence ID" value="XM_037293180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD82"/>
    <dbReference type="GeneID" id="59330667"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF8">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="15">
        <location>
            <begin position="37"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="16">
        <location>
            <begin position="302"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="13">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6221398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="423" mass="44464" checksum="184408354A9EE910" modified="2022-01-19" version="1">MSKAHERLSQVQSHLASSEADIPLGRESILSKSPNDIVITCALRTPFTKGGKGGLKDTPAADLLVHALKSLLARSKLDPSLVEDIAVGSVLPPGGGATEFRAAALVAGFPESTAVKSLNRQCSSGLQACADIANAIRSGMIDIGIGSGVESMSSQYGPGAVTEFSDLLESDPEAKNCKVPMGVLSEQMAKDRSISRKDQDSFAASSYQKALKAQKEGKFDEEIAPLHNVKFVDMKTDEEKVITVDKDDGIRDGITAESLGRIKPAFAKDGSIHAGNASQITDGAAAVLLMKRSTAERLGQNVVGKFVSSSIVGVKPLLMGIGPWKAIPVALEKAGISKDEVDVFEINEAFASQCLWCVRELRLDEKKVNPNGGAIALGHPLGCTGARQVATLFNELGRTGKRIGVTSMCVGTGMGMAAVFVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDF3</accession>
    <name>A0A8H6CDF3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative transcription factor kapC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002173</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150753.1">
        <property type="nucleotide sequence ID" value="XM_037293100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDF3"/>
    <dbReference type="GeneID" id="59330587"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF11">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="10">
        <location>
            <begin position="39"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="146" mass="17184" checksum="6218942673E80D4E" modified="2022-01-19" version="1">MDSQPQVVNSIEFTDLTQPQLEDRRRRRSNTQQDKESVSNMRIRRRAQNRASQRAFRERKEKHVQHLEHELEELEAKHQNLSRSYTDLDSTHAKLKMEVKQLRSELESVKSSREGSVNENPQSNSNFFDPFSSDGNFYGSGTEVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDM3</accession>
    <name>A0A8H6CDM3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001638</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151105.1">
        <property type="nucleotide sequence ID" value="XM_037292566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDM3"/>
    <dbReference type="GeneID" id="59330052"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="142"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="272"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="40524" checksum="30B17B8630E6A20E" modified="2022-01-19" version="1">MASLHFYVTKTRKISASSSSASITAKSPNFQPSGNISEQEFSSHVYRGGIKRLFGGPVEVLRDQIDHYSAITTDAVCEMPLLEAARSGHGKMVKLLIDHGANIEARDHEPETPLRLVAVYGHGAIVKHFVDRSTGLDCLDNNGRTPLSIAAAYGRRAIVTQHIDRSAGLDCLDKDGGTPLSNAADAESTRLAKFWIERGVIAVLLRENGADEMLSPQRDVLRDEARCLAVLVDAEAQGNSEGQLSRARDGHQSNLVTPAEGAQTLVVETEVGQPIDSDTEDSMDGEAPSRSDHQDPEDEAAISVQPRLPPAGTSELAVILALSMVNADHGGVQGDTSHILRDGVEKTKAFPKKYLSDAIFQPRKANHGHHQTPSGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDM8</accession>
    <name>A0A8H6CDM8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002101</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150681.1">
        <property type="nucleotide sequence ID" value="XM_037293028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDM8"/>
    <dbReference type="GeneID" id="59330515"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38794">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38794:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="3">
        <location>
            <begin position="27"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="366" mass="40317" checksum="33819CDEA6B5E0D8" modified="2022-01-19" version="1">MGNENASTVQILTWFLIITSILGVGARGLTKAILVRSVSLDDYLITVALLFAIGQSVAVSVEAGHGYGSPSDPLSRSQATSNLKSEYAASLLYVASLGFAKASVVALVNTLTPIRSHRRATYGLAIFLFLWVIIGEFGTAFLCHLPRPWDYLEGHCSNDTIRRTWWNYFESTNIITDLALICLPIVVIWQIRISLSRKASVCSFFALRSTVIAASACKLVFWNNTQDLQKPGHNPWTVTLCTQIIQCLSIVSACFLYLKPFLDSVESGFIRSDDIRRRGTNEYHRHHTTTTTTTGGSSTTRSAFSTRKHARTGSQSFGLVGVPNPRHITTVTANDPPDFDAQSQHSRTHIIKETRTFAVEGSANEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDV1</accession>
    <name>A0A8H6CDV1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151210.1">
        <property type="nucleotide sequence ID" value="XM_037292671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDV1"/>
    <dbReference type="GeneID" id="59330157"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="85" mass="7870" checksum="84AE60148550C03F" modified="2022-01-19" version="1">MDKVGGVLGGVTDTAGKTVGGATETAGNAVGGVGNTLGDTTKGLTDTVGDTTKGAGSTVSDTTSGVKDSTGAGGKQDAQNPLGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE46</accession>
    <name>A0A8H6CE46_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151227.1">
        <property type="nucleotide sequence ID" value="XM_037292688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE46"/>
    <dbReference type="GeneID" id="59330174"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:iron ion binding"/>
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    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Iron-sulfur assembly protein 2"/>
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        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
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        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
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        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="111"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="56"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF01521"/>
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    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221792.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="227" mass="24590" checksum="F13699464EA818AB" modified="2022-01-19" version="1">MEIRTLLRPLVWVAKHELAFARTASTSNANTQRHSSASQHSYASIPDFLLPSSVTKKHHASTISHRRRPLLQSAPASPGHPLQRRMFSASTAAKAAVVTANPRKDEYGNEMLIDITARAANRLKEIMSKDSNPNLALRVTVESGGCHGFQYLMSLTNTSAISPEDDTIFESSDFSGAKVVMDEPSLELLKGSQVDFTMELIGSQFKIVGNPAATSSCGCGTSFDIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEN9</accession>
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        <recommendedName>
            <fullName evidence="9">MIF4G domain-containing protein</fullName>
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    </protein>
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        <name evidence="10" type="ORF">HO133_001784</name>
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    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic initiation factor 4G family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037292712.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:cytoplasmic stress granule"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:translation initiation factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="eIF4G, eIF4E-binding domain"/>
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        <property type="entry name" value="eIF4G1_eIF4E-bd"/>
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    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
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            <begin position="1086"/>
            <end position="1324"/>
        </location>
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    </feature>
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        <location>
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        <location>
            <begin position="475"/>
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        <location>
            <begin position="532"/>
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        <location>
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        <location>
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        <location>
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        <location>
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        <location>
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        <location>
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        <location>
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        <location>
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005775"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1535" mass="163494" checksum="7D482B922CEE97DA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CES1</accession>
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        <recommendedName>
            <fullName evidence="5">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">HO133_001126</name>
    </gene>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6222040.1"/>
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        <property type="nucleotide sequence ID" value="XM_037292056.1"/>
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    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035003280" description="Peptidase S8/S53 domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="877"/>
        </location>
    </feature>
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        <location>
            <begin position="139"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
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    </feature>
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        <location>
            <begin position="499"/>
            <end position="543"/>
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        <location>
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        <location>
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    </feature>
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        <location>
            <begin position="529"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222040.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="877" mass="92753" checksum="03B040514C42818E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF35</accession>
    <name>A0A8H6CF35_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151476.1">
        <property type="nucleotide sequence ID" value="XM_037292057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF35"/>
    <dbReference type="GeneID" id="59329543"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="234" mass="22783" checksum="0FE05A92FDF402CC" modified="2022-01-19" version="1">MVSTQSKPRGAGSVPIIYNNAPLELCKDCGGSGGYIKRDGSCPLTQIDLPSSTEALPASIAAAIMAGDGGRLGGTAAGMSPTPAAAIGGIIGGVSAGVAAAHASSTLIASPSTSAATTVSCSHAADPHNTCASIASGPGWCVCGESPNSYAVRTSTDLACGWTTPPPTASFDCSAATTTSKPATPPATANPAPSTPPPSSPNAGIAIELQLYSGPAAQKSPHGLPTLTNRETRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG15</accession>
    <name>A0A8H6CG15_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SecE/sec61-gamma protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_000951</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152246.1">
        <property type="nucleotide sequence ID" value="XM_037291884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG15"/>
    <dbReference type="GeneID" id="59329369"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7618" checksum="1D832EB595E8DB5F" modified="2022-01-19" version="1">MSDQFKEILDIPRDFLHDGTQFMNRCTKPDKLEFLKISQAVGVGFVVMGALGYVVKLIHIPVNQVLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGE6</accession>
    <name>A0A8H6CGE6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152395.1">
        <property type="nucleotide sequence ID" value="XM_037292033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGE6"/>
    <dbReference type="GeneID" id="59329519"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034707574" evidence="1">
        <location>
            <begin position="20"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="84" mass="9185" checksum="1C08024ED0BA51B3" modified="2022-01-19" version="1">MQFQTILCLLAATATPMLATPLSLEYNSLQPRSIQSESCQHMGVAIDAVMDRLDDDDCDAKCKTESNKALEALMSVQKEFPCAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH16</accession>
    <name>A0A8H6CH16_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynamin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000206</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152581.1">
        <property type="nucleotide sequence ID" value="XM_037291146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH16"/>
    <dbReference type="GeneID" id="59328625"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001425">
        <property type="entry name" value="Dynamin GTPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF215">
        <property type="entry name" value="DYNAMIN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="4">
        <location>
            <begin position="28"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="419"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="427"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="444"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="620" mass="68618" checksum="306AED52AC1778D4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHB5</accession>
    <name>A0A8H6CHB5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152760.1">
        <property type="nucleotide sequence ID" value="XM_037291325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHB5"/>
    <dbReference type="GeneID" id="59328805"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008418">
        <property type="term" value="F:protein-N-terminal asparagine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070773">
        <property type="term" value="F:protein-N-terminal glutamine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039703">
        <property type="entry name" value="Nta1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750:SF26">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="321" mass="34962" checksum="FC57BC249D63DD02" modified="2022-01-19" version="1">MRIACLQFNPELGRLPENIARANALLEAASPQNIDLLVLPELAFTGYNYPSFSSILPHLEPTASGPSTNWARATASRLNCIVTVGYPELCTPPPASSTKTQDLSPAGEAIAYNSTVTVSPTSQILAHYRKTHLYYTDETWAQESPSGWLSTSLTFAPKTCPERGVRANFGICMDLNPHKFEAPWEAYEFASHALASGSELLVLSMAWLTGLDALELAAHAEEPDLSTLSYWVGRLKPLVEAEKEVIAVFGNRCGQEAGKNPLGVEEGVRYAGSSWIGKVGNGVVKIWDIMGRAEEGVVVADTEKEPKWALRLQSRDKELAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHU1</accession>
    <name>A0A8H6CHU1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152939.1">
        <property type="nucleotide sequence ID" value="XM_037291504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHU1"/>
    <dbReference type="GeneID" id="59328984"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="665" mass="66661" checksum="EEF251AB34A380E9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI72</accession>
    <name>A0A8H6CI72_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152891.1">
        <property type="nucleotide sequence ID" value="XM_037291456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI72"/>
    <dbReference type="GeneID" id="59328936"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="124" mass="13604" checksum="2060F3BE25D804A9" modified="2022-01-19" version="1">MEHTSGHQRAHLPRSLAELDYDQGTDLRHIDDKDLNCHDDHPKPCFLLHGIQAQQLQVIVLTSGVDDGDLFTSEANTPTTASNSTLPSSRGSPTLSQLASSSFRYISHVFCSDSVERGRTQGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIQ8</accession>
    <name>A0A8H6CIQ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153294.1">
        <property type="nucleotide sequence ID" value="XM_037301660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIQ8"/>
    <dbReference type="GeneID" id="59339199"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="89" mass="9608" checksum="266DDA5BD9F94587" modified="2022-01-19" version="1">MSTKYPPPAYPPPAHQDAGPYYPPTQQQQQQQGPYHTGAPTPYQYNGGNAPGYGGQDRGGGWVGAASCWMQFRDERDDTAGAGEFDSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIZ2</accession>
    <name>A0A8H6CIZ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_011005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153488.1">
        <property type="nucleotide sequence ID" value="XM_037301854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIZ2"/>
    <dbReference type="GeneID" id="59339395"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="268" mass="27619" checksum="110F9323ED07BB13" modified="2022-01-19" version="1">MCAGEPIKRPHHLPVVHQCAHIRVTVTKSSVATVTSTLTVASVALKDKRDAGPESALDILPRQVTATPTAIPAYASACSGTVRYSSACSCIGATHSITTVKAPTTTTTVSKTTTITECASPFPTFVLQLENSGIVLNGIALDGTYAQFDDGDGPVIGFNGSPRSQALILSLNAAGNLVMDQNGLIAAIDYGQSMELLHFTDIADAYATEDPAVCSVSGGQLTCSTGAPKILLLCPGFGATNDLFIAVSFEPAPCVLPTIKVVPVCVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ07</accession>
    <name>A0A8H6CJ07_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_011067</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Lipid-anchor</topology>
            <topology evidence="1 8">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153550.1">
        <property type="nucleotide sequence ID" value="XM_037301916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ07"/>
    <dbReference type="GeneID" id="59339457"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF8">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034604268" description="1,3-beta-glucanosyltransferase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="914"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="10">
        <location>
            <begin position="223"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6224490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="932" mass="100401" checksum="D1C521823BA3EF70" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJG9</accession>
    <name>A0A8H6CJG9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009939</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153612.1">
        <property type="nucleotide sequence ID" value="XM_037300795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJG9"/>
    <dbReference type="GeneID" id="59338331"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045862">
        <property type="term" value="P:positive regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="5"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="7">
        <location>
            <begin position="203"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="32531" checksum="6FD7D17E9D2831E5" modified="2022-01-19" version="1">MAHRAVALKEEGNKYFQNGEYTEAEGLYSQAIQKDPSNPKLFTNRAMTRIKLQQFENCMDDCLKSIELDRVNMKGYYLLAQTQLALNHPNEALTSAITAYEKCLETNDKSTSAVSGLVLQAKKQKWEAKERERIRNRSSMLRELEDMLMKNKREELRQLRLKMLDFNEEAEEKSDIELECRKKVEELRTIFAIADPKNLQRREVPDYLIDSISFAVMHDPVTTKTGNSYDRVTLMEHFKRSHTDPLTREPLRADDIRPNLALKQACEEFLENNGWAVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJW8</accession>
    <name>A0A8H6CJW8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153639.1">
        <property type="nucleotide sequence ID" value="XM_037300822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJW8"/>
    <dbReference type="GeneID" id="59338358"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="241" mass="26736" checksum="C78A81135ACA21EE" modified="2022-01-19" version="1">MQAFLQTLQVYDNERGNKPSYFVTEEDCKFFLQQAKILQDRAAYIRQEWARLEALQATQSALPTPPAQQSTQSITQQATQPITQQEEEPLQQANAENTAEKIAENTVENTAEKPSGSKAPITKNTGLLSTLPCRIATQQTENTTAIWPVIPTALIWYITAMTSQEEDLLRRSQPIGPSTGGSSDDTYLDELKPSDVLLQHPFHYSKAFTAAGDFAGCTPLISLASPFISGIRRIMHAYFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK61</accession>
    <name>A0A8H6CK61_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010316</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tryptophan dimethylallyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153987.1">
        <property type="nucleotide sequence ID" value="XM_037301170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK61"/>
    <dbReference type="GeneID" id="59338707"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009820">
        <property type="term" value="P:alkaloid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02231">
        <property type="entry name" value="cupin_BLL6423-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13929">
        <property type="entry name" value="PT-DMATS_CymD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033964">
        <property type="entry name" value="ABBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017795">
        <property type="entry name" value="ABBA_NscD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03429">
        <property type="entry name" value="arom_pren_DMATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40627">
        <property type="entry name" value="INDOLE PRENYLTRANSFERASE TDIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40627:SF4">
        <property type="entry name" value="PRENYLTRANSFERASE ASQH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11991">
        <property type="entry name" value="Trp_DMAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00036">
        <property type="entry name" value="Aromatic_Prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01162">
        <property type="entry name" value="I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="687" mass="75550" checksum="A8D22A850EE122CA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKK9</accession>
    <name>A0A8H6CKK9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAD superfamily hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153907.1">
        <property type="nucleotide sequence ID" value="XM_037301090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKK9"/>
    <dbReference type="GeneID" id="59338627"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07505">
        <property type="entry name" value="HAD_BPGM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044999">
        <property type="entry name" value="CbbY-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896:SF2">
        <property type="entry name" value="CBBY-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896">
        <property type="entry name" value="XYLULOSE-1,5-BISPHOSPHATE (XUBP) PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="26889" checksum="11974BE68FBF06A7" modified="2022-01-19" version="1">MPKITEILFDCDNTLVLSEELAFEACADLANEILAKYNIPDRYTGPELLNDFVGQNFRGMVVNLQKKFDFSIPGPEMEKYVSMEEDRVIAKLERDAKPCVGCSEELDKLYKAKKYGMAVVSSSALRRVQASIDKVDQAKYFPKDHIFSAATSLPKPTTKPDPAIYLHACKVIGKEPGECVAVEDSKSGTLSAVRAGIPVIGYVGSYGGSEKQKEMTETLEGLGVGIVMQNWDEFPQCLEAIEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKR3</accession>
    <name>A0A8H6CKR3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010476</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154145.1">
        <property type="nucleotide sequence ID" value="XM_037301328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKR3"/>
    <dbReference type="GeneID" id="59338866"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="852"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="889"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="666"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="676"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="910" mass="101631" checksum="370ECF947FBF73D8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL26</accession>
    <name>A0A8H6CL26_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DUF2428 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010343</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154013.1">
        <property type="nucleotide sequence ID" value="XM_037301196.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338734"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056843">
        <property type="entry name" value="THADA-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25150">
        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26523">
        <property type="entry name" value="Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1546"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="6">
        <location>
            <begin position="311"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="5">
        <location>
            <begin position="643"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="7">
        <location>
            <begin position="877"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="499"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="503"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1596" mass="177833" checksum="892EF2B46E384E75" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLJ7</accession>
    <name>A0A8H6CLJ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7779 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154155.1">
        <property type="nucleotide sequence ID" value="XM_037300307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLJ7"/>
    <dbReference type="GeneID" id="59337841"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF7779"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR35205">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35205:SF1">
        <property type="entry name" value="ZU5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25000">
        <property type="entry name" value="DUF7779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7779" evidence="1">
        <location>
            <begin position="377"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="940" mass="105295" checksum="0AC070105662475A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLJ8</accession>
    <name>A0A8H6CLJ8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154443.1">
        <property type="nucleotide sequence ID" value="XM_037300595.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338131"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="889"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="647"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="728"/>
        </location>
    </feature>
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        <location>
            <begin position="731"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="860"/>
            <end position="869"/>
        </location>
    </feature>
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        <location>
            <begin position="939"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1128"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1147"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1200"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1330"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1410"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1469"/>
            <end position="1482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1578" mass="166166" checksum="2FD23FA69D35D95C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CML7</accession>
    <name>A0A8H6CML7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154733.1">
        <property type="nucleotide sequence ID" value="XM_037299907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CML7"/>
    <dbReference type="GeneID" id="59337441"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1230.10:FF:000005">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone]alpha subcomplex assembly factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1230.10">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192">
        <property type="entry name" value="NUCLEAR PROTEIN E3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="25766" checksum="77DB9EE25BB13E9B" modified="2022-01-19" version="1">MAAPSIPLLRALRATEAIRSTLPLRQQPPNLPKRFSSHSPSPHRRSPHPFQTTNLRSHARTLSTTPTPLSASSPPTTTDRGPASTEPTQTDFSAMDVFSNAPAPTASIDACLSDGFHLDNGVKITGGAGLLLVSGEAFAWRPWEGGGRGRLLNSKGQWDTGEGAWGVLGLVWPKPDLLILGLGPGMYPIAPETRRCINDMGIRIDVQDTRNAAAQFNLLATERGVGNVACALIPIGWREPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMV7</accession>
    <name>A0A8H6CMV7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154885.1">
        <property type="nucleotide sequence ID" value="XM_037300059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMV7"/>
    <dbReference type="GeneID" id="59337593"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06262">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF4">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE MBLAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="316" mass="36195" checksum="E5E66978CB099D50" modified="2022-01-19" version="1">MAANPSRSQYFTCSRLNATTFVVYENDKYEEHPFIYVKIYDDPRLVVISDTGCGGGDEDREHYHNLKKYIETCPAAANDGKPLNPIEARRTYFIICTHCHYDHILGLKHFQTASTSILASSFDRHFIEKNLPVHSLCEFLDVPTPEYTVSYWADDLDKLSFDGKPLHLQILHTPGHTPDELAWYDEQERHLYVGDSFYERVAKDNSYEQAIIFPKEGNWVHYMQSLEKLLRFVEEKNEEPGKATIRIGCGHITSSVDGVEILIIVQQLFQDIIAGKAPIVHLEEKRGEEYALWKADGEPRFSVAAPKRLALEATKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNE2</accession>
    <name>A0A8H6CNE2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_009272</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154959.1">
        <property type="nucleotide sequence ID" value="XM_037300133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNE2"/>
    <dbReference type="GeneID" id="59337667"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001463">
        <property type="entry name" value="Na/Ala_symport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF55">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00175">
        <property type="entry name" value="NAALASMPORT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="574"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="603"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="640"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="676"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="336"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="336"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6226406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="715" mass="80310" checksum="618B0A335EC87FC1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNI7</accession>
    <name>A0A8H6CNI7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase family 28 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009332</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155019.1">
        <property type="nucleotide sequence ID" value="XM_037300193.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337727"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016906">
        <property type="term" value="F:sterol 3-beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000100">
        <property type="entry name" value="Glycosyltransferase family 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000009">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase UGT80A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010610">
        <property type="entry name" value="EryCIII-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050426">
        <property type="entry name" value="Glycosyltransferase_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050:SF13">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE UGT80A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06722">
        <property type="entry name" value="EryCIII-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase family 28 N-terminal" evidence="3">
        <location>
            <begin position="178"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Erythromycin biosynthesis protein CIII-like C-terminal" evidence="4">
        <location>
            <begin position="512"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="734"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1044"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1149"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1188"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="813"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1044"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1203"/>
            <end position="1214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1293" mass="138903" checksum="81A07B48663D9B1C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNT4</accession>
    <name>A0A8H6CNT4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008268</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155136.1">
        <property type="nucleotide sequence ID" value="XM_037299135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNT4"/>
    <dbReference type="GeneID" id="59336665"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052210">
        <property type="entry name" value="LysM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34997">
        <property type="entry name" value="AM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34997:SF1">
        <property type="entry name" value="PEPTIDOGLYCAN-BINDING LYSIN DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034617739" description="LysM domain-containing protein" evidence="4">
        <location>
            <begin position="28"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="5">
        <location>
            <begin position="60"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="109"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="154" mass="15917" checksum="8983934308890A54" modified="2022-01-19" version="1">MRSSSGMSTIIQTYIFFALSITSEVQGFRNDQAGITPPPAVATLVARDQVVLGACGPFDHWITVAAGASCESVASDNEITMDEFLLMNPNISPSCDNLIAGNQYCIGTGPASPPSSTIPSSTSTPPPPPSTSAGPTPTPTTTPPSSPINNFQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP44</accession>
    <name>A0A8H6CP44_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008692</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155558.1">
        <property type="nucleotide sequence ID" value="XM_037299557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP44"/>
    <dbReference type="GeneID" id="59337087"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="313" mass="36006" checksum="C506F74A5F4D8BEC" modified="2022-01-19" version="1">MPTLDGQDDLLDELILAKQWKQALSLCEKKLKKANSSDYLLVKKIKVLLLWPEKTRHQQGLKELGVLLERNPPVVDIETLRTLDTLAEAKGQNEPRLRQVWQRAAGVRPQDEKLHVVWFRSKFQVGNLRAAQQASQSWMKNFPKNREPFFLYILTMHKLAEDSATPESERTLLRSLAYKFLSKAASEVSDGVEGIKPTRAINKLEDLLLLIRVYRAQGKYSEAAAIVRGSRMGMESPLGRNSWELVRHLIELLEMSHQWVDSWQLCQQLLVDARDESTERRSHLTHYPFGKLGDDWKVWQALITASSNIGTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP52</accession>
    <name>A0A8H6CP52_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008431</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155298.1">
        <property type="nucleotide sequence ID" value="XM_037299297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP52"/>
    <dbReference type="GeneID" id="59336827"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11060">
        <property type="entry name" value="CYP57A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF232">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="64312" checksum="282D36CE8A1BEDF8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPM5</accession>
    <name>A0A8H6CPM5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Meiotic expression up-regulated protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008377</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155244.1">
        <property type="nucleotide sequence ID" value="XM_037299243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPM5"/>
    <dbReference type="GeneID" id="59336773"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000005">
        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF1">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="276"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="336"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="39000" checksum="1E99180D65528CF0" modified="2022-01-19" version="1">MHRTYSMRQSRAPTASQIQNPPPPSSSTKSGRFFGKGGVGHAFRKSTAGAFGPDLAKKLAILVKMEKNVMRSMELVGRERMEVAQQLSIWGEACDDDVSDVTDKLGVLIYEVGELEDQLVDRYDQYRVTIKSIRNIEASVQPSRDRKQKITDQIAQLKYKEPNSPRIVVLEQELVRAEAESLVAEAQLSNITREKLKAAFTYQFDALKEHSEKLAIIAGFGKHLLELVDDTPVTPGETREAYDGYEASKAIIQDCEDALTNWVTANAAIKPSLSTRARNLSQRRKSNMGEGVGHDLSGQDSSMKDRESGLWVPAGAHREYSNEEEEEEEEEEEPSAETRGREEERPAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR18</accession>
    <name>A0A8H6CR18_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155932.1">
        <property type="nucleotide sequence ID" value="XM_037298596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR18"/>
    <dbReference type="GeneID" id="59336123"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053221">
        <property type="entry name" value="Burnettramic_acid_biosynth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38887">
        <property type="entry name" value="CHROMOSOME 21, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38887:SF1">
        <property type="entry name" value="RAS MODIFICATION PROTEIN ERF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="497" mass="54677" checksum="8E2C5A2AD5CD7780" modified="2022-01-19" version="1">MGVLVRLIGSGVGLAAEAIASKKQRSRSNSPGANLSPQASASSSRDIGPSSSSNKSEKKSSKAREAHEDFEEPPPAYEQIVEVPEERADELIADGKALPLDEKHGPSQEREAHEDDSDSDSEGDEANWALDEAAEEVATPGAEDPKLKYGMMAQPTIESFSAVVMAKCPPLNHPTNPLQRPVIIPQRRPGARDRGFIHAYSPDLAEKGISEEAFLSFIENFHAASQASPLLNVVFVSAGVVGLVPEPISQITSIIIQFAAGAAIYMQTTQRTHSFLDEMNDKLFKPRGLYAMVMKYIPQGTRPISVQQADLNTIISKENAPHKSYQNFRAVNAGRSYGAIEMPDSAPLVFPALDEAAQTQDPAKASKLKSSMAFVTDYYDKRAQASYLHKNPDQRLDLPKPDFGTRVSDPNHPAFRGSWLTLISGGKIPPKAERRARRREERIQAGVAPSRKQERKAWKQKRKLMKQNVLYLMIVNLPTEQEMEAAKMQVQQQAGMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR22</accession>
    <name>A0A8H6CR22_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008045</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156249.1">
        <property type="nucleotide sequence ID" value="XM_037298913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR22"/>
    <dbReference type="GeneID" id="59336442"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF36">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G08340)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="54608" checksum="A1D1056D4532E5BD" modified="2022-01-19" version="1">MTKDLHLVGNDYQWLLTIFYITYIVFEFQALMWKIVPPHIWLAFTVFGWGLIASAQSGTNTWSGMMACRFFLGISEAGFGPGIPYLLSFFYLRHEVGLRIAIFLSAAPLATSFSGALAYGITSGYPHLAKWRLLFLVEGLPTICMAPVAFFFLPDTPDKARFLTEEEKLIAKARGVRQVGSVERLGGIVWKEVGLALMDVKCWFTAFMYFSCNVGFSSLPVFLPTILNDMGFNAIDSQGLTAPPFFVSFLVTILSTWVADKTQQRGYTIMVLTTVGGIGYIMLATVKSVGVRYFGVFLAASGIFPSIANILPWVTNNQGSDTRRGMGIVLLNLIGQCGPLLGTNSFPMNESPRYIKGMAISAAFTFFTGFLAFGLRCLLVWENRKLDKLHGSNKNRVVNQPGGEGQESEVGEENYGPTFRDEDMYNMSESMKLAQHRSERFNMMWKAWFSFRVVDWLDLNGPWTSDEKLDRFTKTGVGNSLGMVEQHAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR44</accession>
    <name>A0A8H6CR44_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Ubiquitin-like modifier-activating enzyme ATG7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Autophagy-related protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_008075</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156279.1">
        <property type="nucleotide sequence ID" value="XM_037298943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR44"/>
    <dbReference type="GeneID" id="59336472"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019778">
        <property type="term" value="F:Atg12 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019779">
        <property type="term" value="F:Atg8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01486">
        <property type="entry name" value="Apg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.70:FF:000001">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme atg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000243">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.100">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.70">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006285">
        <property type="entry name" value="Atg7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032197">
        <property type="entry name" value="Atg7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042522">
        <property type="entry name" value="Atg7_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042523">
        <property type="entry name" value="Atg7_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01381">
        <property type="entry name" value="E1_like_apg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16420">
        <property type="entry name" value="ATG7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like modifier-activating enzyme Atg7 N-terminal" evidence="10">
        <location>
            <begin position="2"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="9">
        <location>
            <begin position="392"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606285-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6228345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="729" mass="80352" checksum="08BF7F064C85D566" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRE3</accession>
    <name>A0A8H6CRE3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007664</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155870.1">
        <property type="nucleotide sequence ID" value="XM_037298534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRE3"/>
    <dbReference type="GeneID" id="59336061"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="34249" checksum="A4DAFBF2F1747B11" modified="2022-01-19" version="1">MLLSHTGDLKIKNDCTFAGLQQAAVVAKEAVVKMIPEGYVDPEVRCRYAAALLRKAASNPHTSALQLLLEHAHLAKASDKCTGIQNVLWAAASVNNEAATRLLLDAGADPNHQADKRKDPVMHCSIRNRRRRFGSLGVVEMLKIREATVQTRLEHGDSTEVPRNTTLYHHRGCTLLIVTACEDSCRRILQLLLDNGPNREANDTEGKTALYYAAGFGAIAHVRLLLERGAYPTSVNLATIELRREVDQDDYDEGIRLIRDARLEKKAESYTESEMENQVMEGQTESTEVVYSQTTTNLAQEMMTSLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6E9</accession>
    <name>A0A8H6F6E9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ecp2 effector protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146897.1">
        <property type="nucleotide sequence ID" value="XM_037297696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6E9"/>
    <dbReference type="GeneID" id="59335200"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034517611" description="Ecp2 effector protein domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="34681" checksum="758C76EED99E057D" modified="2022-01-19" version="1">MSSLFSYLATYYLLPTFVISLALHPPQIRDGSITLTKPSLLLPSLNASAGSENSNTMPILTWAKVSCDDRSFGNPPAASCKDALAQIPQDPATIITDPERSYGPRGKGSWDVNLPKRYVGSDGQCIIDLIQTSYPSHVRNDDLLGAATIIQQECVEKAIPPKGGVVFDMADELNFVLLMQSWSPPFVRCSNTPLATRPVSASCQTILDTMNVSIEQTTFGAVGIAGVEEHLPQVLTEREHHCPLLSMKRFFLLKSAACSLATGQCSITVDTTAPDDAASWFDLWQAAVALDGMCARGGRAGKARFLGESMLNECCLVSYEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7K5</accession>
    <name>A0A8H6F7K5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006323</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147346.1">
        <property type="nucleotide sequence ID" value="XM_037297221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7K5"/>
    <dbReference type="GeneID" id="59334724"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="159" mass="17148" checksum="24AEE067A58E15A8" modified="2022-01-19" version="1">MAQGAKDPSSAASTDSESPQTSPGAVFGNIFVFIKARYETSANTLKDAVTLLACRPDIQKALRADLGKTKSSVTILDGYVEATVDETLRLYTLLPIFPKTTREIPHSFSISSSGYTIPADTLILMNTSATHRNPKYWPAAVPTAGDGPPYRVPSFDRSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7L1</accession>
    <name>A0A8H6F7L1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006139</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147615.1">
        <property type="nucleotide sequence ID" value="XM_037297041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7L1"/>
    <dbReference type="GeneID" id="59334543"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF73">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0187">Copper transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="221" mass="24356" checksum="95BC379BCD6880AD" modified="2022-01-19" version="1">MDMMTTSMGSAMVTKTPGIPIPSSATGAPEMSMPGMPGMDMGSSVNCKSSMFWNWYTLDACFLSTTWHITSRGMFVGSCICVIGLVLLLEFSRRLQREYDRLIQQHATYDYEAGRAFNTSKSDRKGSADSNDISNMEDPPHSAVPLLGEWERSLPLRAPGHVPSVLQQAIRAGIYTLQFSVAYLIMLLAMYYNGYILVCVFIGAFIGFFIFSWDASGAASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7N3</accession>
    <name>A0A8H6F7N3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-hexosaminidase</fullName>
            <ecNumber evidence="7">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_006511</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147119.1">
        <property type="nucleotide sequence ID" value="XM_037297409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7N3"/>
    <dbReference type="GeneID" id="59334912"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016231">
        <property type="term" value="F:beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030203">
        <property type="term" value="P:glycosaminoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000063">
        <property type="entry name" value="Beta-hexosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029019">
        <property type="entry name" value="HEX_eukaryotic_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600:SF58">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14845">
        <property type="entry name" value="Glycohydro_20b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001093">
        <property type="entry name" value="B-hxosamndse_ab_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-hexosaminidase eukaryotic type N-terminal" evidence="11">
        <location>
            <begin position="30"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="10">
        <location>
            <begin position="206"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001093-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6217684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="594" mass="66104" checksum="F55AAA489CD1962A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8F4</accession>
    <name>A0A8H6F8F4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005828</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147914.1">
        <property type="nucleotide sequence ID" value="XM_037296733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8F4"/>
    <dbReference type="GeneID" id="59334233"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="222" mass="23527" checksum="756722E74807358A" modified="2022-01-19" version="1">MPLKDDDGIAPPVPASIRAGRVPPPSSQGIFFLTGALAGATTIPIESLWQRLVLRAPGSLPLLGWAPIYRGGVRFWTFDLARYRVERLPIPVAIKGGLSGAAGGLAEVCVQSLFRNKLPDMASLTSQSGKMFCCFGTYTFLSTTLSPEQLPPKPFWYCWLIGATAGGLGSGIIARAEGVTGSALWRVAVPKGALTIGTVIAVQVTTCAVILPYNRFIPNGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8G8</accession>
    <name>A0A8H6F8G8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004982</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148592.1">
        <property type="nucleotide sequence ID" value="XM_037295894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8G8"/>
    <dbReference type="GeneID" id="59333388"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034178669" evidence="2">
        <location>
            <begin position="20"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="829" mass="80926" checksum="845B6AABAA877093" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9R1</accession>
    <name>A0A8H6F9R1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NACHT-NTPase and P-loop NTPases N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149942.1">
        <property type="nucleotide sequence ID" value="XM_037293864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9R1"/>
    <dbReference type="GeneID" id="59331352"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031352">
        <property type="entry name" value="SesA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17107">
        <property type="entry name" value="SesA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT-NTPase and P-loop NTPases N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="318"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="544" mass="61367" checksum="AA51E6569C28E682" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA38</accession>
    <name>A0A8H6FA38_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150332.1">
        <property type="nucleotide sequence ID" value="XM_037293503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA38"/>
    <dbReference type="GeneID" id="59330990"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="503"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="625" mass="69081" checksum="FFE1684D25C12101" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA54</accession>
    <name>A0A8H6FA54_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_002545</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150300.1">
        <property type="nucleotide sequence ID" value="XM_037293471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA54"/>
    <dbReference type="GeneID" id="59330958"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11609">
        <property type="entry name" value="MCT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21155">
        <property type="entry name" value="PUA_MCTS-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="261"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6220865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="649" mass="72271" checksum="25D732D82EDFA1FA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAH4</accession>
    <name>A0A8H6FAH4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150675.1">
        <property type="nucleotide sequence ID" value="XM_037293022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAH4"/>
    <dbReference type="GeneID" id="59330509"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="325" mass="36841" checksum="9D842ED77CD55475" modified="2022-01-19" version="1">MGKKEIVPPPEPKNKLFFSFGPDETSFFSSPLYRRWSENSPEEFKKLLPDADPNKPEPETPPLEGHKFFSSMKRAMSHKKEETVEPQPYHVSYGDNGSWFLIWDNGEHDENLRGAYPSLLQWMHENQSSLTDGSLNVSLGPKGAFFAWCTESCRWDNVPASFTAEIQKMLCHDGWKRRPRRVQFGVNESYVIVCDDGTWYPHGLEENYEGLMELLTSSGKMKTLSLDAYGPDTYFLVSETGNVHWKVPQTWDNVVEDVSNTYMTGHRVRTGSMPMDKDLTYSKESKQGKGKANPGTMTKWLSGANAALQVGQLVITADPGQCQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAM9</accession>
    <name>A0A8H6FAM9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150224.1">
        <property type="nucleotide sequence ID" value="XM_037293395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAM9"/>
    <dbReference type="GeneID" id="59330882"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="380" mass="41911" checksum="E969166A155B328D" modified="2022-01-19" version="1">MAEQWPHQQDGREHSMSDGIHSVDVRSLDPEYLSACVLFAQSHGIYPSSSARHPDNDIASNSLNALPEGDYQSTDLAVDHQGPNRGPFSTHDPTLYTSNTFADTSYPQHDRCQYSSPYTETTAADPIYNQRSSSFPISNHDDINLDPSLPHQHSYHPQHQASSPYANDATAPAQARKHHSTPAPTKLHRKITQPRLRFVTNEVGDNGFATLHNYDPQWVQSTGADPNSVFESYSKMKFEKLFLAGVIQKADRLVIRYTGAFSSVVDNGVATLTHVGFSRGSKRNILFPDLALTLSSHPSYLRTISACSGPGAIMTHFAQCNITGPDIGANLARHLHVWRGSQGLGTLKYVRQAYHTWKKDMETDRDGADGNGVTKEGEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAR6</accession>
    <name>A0A8H6FAR6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002589</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150344.1">
        <property type="nucleotide sequence ID" value="XM_037293515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAR6"/>
    <dbReference type="GeneID" id="59331002"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24029" checksum="FB406E4E97416BFD" modified="2022-01-19" version="1">MAPNTFTLAADNSPHLLPILRANPSLASAQDDHGYSLVHAAASYNHLDLLQTLVHEFHVDPNIRDEDGETALFVVESVEPAQCLLEELGADLTLKNEEGMTAEEKIRAEGEYPTIADFLRESRVIGTPAVGDTQSTQNGSHPPPLPPNVKVHLGTMEDPQAIGEGTEADPEFRRRIEELAARDDFQGEEGQKQLRDLIKDAIRGAGREDERQVRPRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB45</accession>
    <name>A0A8H6FB45_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_002154</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150734.1">
        <property type="nucleotide sequence ID" value="XM_037293081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB45"/>
    <dbReference type="GeneID" id="59330568"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF57">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="35"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="152"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="249"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6221299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38586" checksum="55999D225D595B0C" modified="2022-01-19" version="1">MSSVQTSIVNPDRKNATNEAIGQGQPARSAQHKNYKGFVAGVFSGISKLSGLMFVSFDTIKVRLQTSEKVQFKGPLDCLLQTLRHEGVRGLYKGATPPLVGWMFMDSIMLGSLTFYRRILKDNVFSNPSILTISRATSRKFENTNRQVEDSLPTTGHGIAGIMAGGTVSFIAAPVEHVKARLQVQYAADKSKRLYSGPIDCITKIARAHGIRGIYHGLSATLLFRSFFFFWWGSYDLFTRYFSDHTQLSTPSTNFWAGGLSAQVFWLTSYPSDVVKQRIMTDPLGGALRDGERRFPRWKDAAKEIWRRDGPRGFWRGFVPCFLRAFPANAFALVAFEGVMRGLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCK2</accession>
    <name>A0A8H6FCK2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal eL28/Mak16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152664.1">
        <property type="nucleotide sequence ID" value="XM_037291229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCK2"/>
    <dbReference type="GeneID" id="59328709"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.110:FF:000002">
        <property type="entry name" value="60S ribosomal protein L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002672">
        <property type="entry name" value="Ribosomal_eL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10544">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="5">
        <location>
            <begin position="69"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="187"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="213" mass="23878" checksum="F2D853DF5416BA7C" modified="2022-01-19" version="1">MHDQLDYFLTLYDSYLRCNGYLSLSAVGALLFSTEPSPTKVVLRFDLISIAELHFKMTEHLQSNVSQDLVWEICRPNNAFLVKRKSGGGSQFSRDPLNLMNKHSRKYAGFVNNKAVGIHPAEKGGVTLATKKTKHLNRPAVNKNEVSWSGQKSGPKIYKGIVNYTAKQGYRADLRQEAVARASAIRESQRPKKEDPEPKIRGSKAKKAEEKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCV8</accession>
    <name>A0A8H6FCV8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000228</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152603.1">
        <property type="nucleotide sequence ID" value="XM_037291168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCV8"/>
    <dbReference type="GeneID" id="59328647"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38794">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38794:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="3">
        <location>
            <begin position="35"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="403" mass="44013" checksum="FEBED2250EDB62BC" modified="2022-01-19" version="1">MSAAIPSDSYDSYGRIVNVISWILLCVMVLTVGTRAATKWTLIRRLEGDDVLALIAFTFSIGQTVALSEAISNGLGEHLGALSPSQVRNFQQSVYAADLLYIVVLWSAKTSVLLLVRQITPVALHKRITLYVGCGLALWAAVAILAAAVQCHTPHVWQFLDNQCFDRYAFWIYIGISSIVGDTVLIIIPIAIFWNVQIDRRRKATVCGCFASRILVIIATGLQLGFSGRAHGSTDLTLDLWPPALCTQFVQNLSIMTACVPYLKPFYLGLESGMIRTDDLRRHGLIGTYGYGDDESAKVSSRRGPPNTVGSAKSTTSHELRSLDVETAARSAQLNPNNLVAVEASNGVKAADWDQESQTSQSRIIKQTRTWGVDFESQDSNPEGLPVPAWTQGTWGKSSYQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD67</accession>
    <name>A0A8H6FD67_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Transcription elongation factor SPT4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_000377</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SPT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152751.1">
        <property type="nucleotide sequence ID" value="XM_037291316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD67"/>
    <dbReference type="GeneID" id="59328796"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07973">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009287">
        <property type="entry name" value="Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038510">
        <property type="entry name" value="Spt4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882">
        <property type="entry name" value="SUPPRESSOR OF TY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025023">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0137">Centromere</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="9">
        <location>
            <begin position="14"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR025023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6223534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="122" mass="13209" checksum="F7B3324BBC0982A4" modified="2022-01-19" version="1">MSSAFVPGSQQRYLRACMVCSIVQTHARFLRDGCPNCESFLGLANSSDAVQECTSQVFEGLITLADPTTSWVARWQRLEGCVSGVYAVKVVGTLPDDVKQNIEDAGARYIPRDGTADDDDTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDB1</accession>
    <name>A0A8H6FDB1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_000496</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152870.1">
        <property type="nucleotide sequence ID" value="XM_037291435.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59328915"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002416">
        <property type="entry name" value="ABC multidrug transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="614"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="643"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="684"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="720"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="750"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="859"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1354"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1399"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1426"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1552"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="248"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="948"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="58"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="905"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6223653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1600" mass="178743" checksum="0DC04A0BF16897AF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FES9</accession>
    <name>A0A8H6FES9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009849</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154556.1">
        <property type="nucleotide sequence ID" value="XM_037300708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FES9"/>
    <dbReference type="GeneID" id="59338244"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034097567" evidence="2">
        <location>
            <begin position="26"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="448" mass="47143" checksum="92C4E6F26F9EF144" modified="2022-01-19" version="1">MVFSSKAVLLAAPIFTLLFSTIVTTSPLEYAENHGEANDALVILRRQVADPTNVAPEDETDAEIAADGTRPLRKAVTARSIYLDQATATWYQIDVASNDLAASFTSFTDTTATTSIPPPDDPKQTSGPNPWNVDHIFELQIIGEAFKADRPTDNPTSIAAADWTAVTNAVFTLSPACTAIAEEVTVLQNLEGIPNAVNSFKKQVFTGNLTGVGFPSTGNTATYYSFFGPAVQKYLVSNQPNFFSVNGIVDNIGDQLSSAGNNNAAIKAYFTSYAAFHYQGAIDYLSTWSGKPITRTIASSTAATGTSAAAATVTCQHNADPDNTCAAIADSLGYCECNNDGNTYAIEPSAPNPCGWTTLPPTTSFDCSSTPPPAPVVVTPTTTPLACTLRDEVIGGGRDPEITHICTCNDGSSPSATVSGDEYACPAQVSIIAKRSPWNGIDLGNFAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEU1</accession>
    <name>A0A8H6FEU1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_010455</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154125.1">
        <property type="nucleotide sequence ID" value="XM_037301308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEU1"/>
    <dbReference type="GeneID" id="59338846"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15845">
        <property type="entry name" value="SNARE_syntaxin16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000021">
        <property type="entry name" value="SNARE complex subunit (Tlg2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF83">
        <property type="entry name" value="SYNTAXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="240"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="339"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6225258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="382" mass="42671" checksum="1EF4DF6C405007E0" modified="2022-01-19" version="1">MSGVWRDRTNLYISYRQSYAHHPAKKRKTIGPSANGFSTFGYESEERRGLMSAGAFEDDGDAVIEMDLLPPRWVDIQEEVTDLLGTIAKKSTDLEKLHQKHVLPGFDDEDVKKQEEIAIERITQEITRGFHDCQKAIQRIEMMVRDARHQGGVSAADETMARNIQISLASRVQEVSAGFRKKQSSYLKKLRTLGGLTFDRTSAPSQNPYTDPSMMESETDKSFSQSTLQQTSMKRLKTNDVAIAQREREIEDIAQGIIELADIFKELQGMVIDQGTMLDRIDYNIENMSVNVKAADKELNVATGYQKTYTKRKILFLLFLLVIGMFILLLVKPKRTKAPLPPPSADPIGTLGEAKPQPEIAASKPDIVQPNGGNVGSDGLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF59</accession>
    <name>A0A8H6FF59_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009088</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154775.1">
        <property type="nucleotide sequence ID" value="XM_037299949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF59"/>
    <dbReference type="GeneID" id="59337483"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000006">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="7">
        <location>
            <begin position="41"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="23367" checksum="0B1FC7AA1796DEB4" modified="2022-01-19" version="1">MASTMPNFLTRLFRPFTTSTRMSLNPEGRSTADFPANAQKCTVAAGCFWGVEHLFRKTFEGKGLLDARVGYIGGDTSSPSYRAVCSGRTGHAESLQVTFDPSTLSYATLLEFFYKMHDPTTPNRQGMDTGTQYRSAIFYHSPEQKAEAEGVTQKVQRQWWKQGTVSTEILEAGQWWDAEDYHQLYLHNNPGGYECPAHFVRKFPDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFE4</accession>
    <name>A0A8H6FFE4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Autophagy-related protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009860</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in cytoplasm to vacuole transport (Cvt), pexophagy, mitophagy and nucleophagy. Recruits mitochondria for their selective degradation via autophagy (mitophagy) during starvation. Works as scaffold proteins that recruit ATG proteins to the pre-autophagosome (PAS), the site of vesicle/autophagosome formation. Required for the Cvt vesicles completion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Preautophagosomal structure membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Vacuole membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="7">During pexophagy, accumulates in the vacuolar membrane region, where the peroxisomes contact the vacuole.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATG11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154567.1">
        <property type="nucleotide sequence ID" value="XM_037300719.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338255"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903599">
        <property type="term" value="P:positive regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019460">
        <property type="entry name" value="Atg11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10377">
        <property type="entry name" value="ATG11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="10">
        <location>
            <begin position="115"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy-related protein 11 C-terminal" evidence="11">
        <location>
            <begin position="1124"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="587"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1233"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1297"/>
            <end position="1343"/>
        </location>
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        <location>
            <begin position="1355"/>
            <end position="1426"/>
        </location>
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        <location>
            <begin position="560"/>
            <end position="587"/>
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    </feature>
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        <location>
            <begin position="650"/>
            <end position="817"/>
        </location>
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        <location>
            <begin position="867"/>
            <end position="894"/>
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        <location>
            <begin position="972"/>
            <end position="999"/>
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            <begin position="611"/>
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            <end position="1326"/>
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            <begin position="1328"/>
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            <end position="1425"/>
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        </source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1443" mass="161247" checksum="BDD1C3AA9A8546A8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFL0</accession>
    <name>A0A8H6FFL0_9LECA</name>
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        <recommendedName>
            <fullName evidence="6">Cryptic loci regulator 2 N-terminal domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="4" type="ORF">HO133_009386</name>
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        <name type="scientific">Letharia lupina</name>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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            <end position="625"/>
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            <end position="234"/>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="817" mass="93735" checksum="CF329DF62DF91825" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFM4</accession>
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            <fullName evidence="6">FYVE-type domain-containing protein</fullName>
        </recommendedName>
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        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
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            <taxon>Lecanorales</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037300083.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59337617"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164:SF30">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="6">
        <location>
            <begin position="93"/>
            <end position="207"/>
        </location>
    </feature>
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        <location>
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            <end position="81"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="243"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="334"/>
            <end position="376"/>
        </location>
    </feature>
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        <location>
            <begin position="434"/>
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        <location>
            <begin position="32"/>
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    </feature>
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        <location>
            <begin position="243"/>
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    </feature>
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        <location>
            <begin position="453"/>
            <end position="462"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="523" mass="56846" checksum="46C0D96F78113EC9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG54</accession>
    <name>A0A8H6FG54_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">O-methyltransferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">HO133_008322</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155190.1">
        <property type="nucleotide sequence ID" value="XM_037299189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG54"/>
    <dbReference type="GeneID" id="59336719"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF1">
        <property type="entry name" value="HYPOTHETICAL O-METHYLTRANSFERASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="120"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="302" mass="34047" checksum="E1B4675243BD94BE" modified="2022-01-19" version="1">MNIVGETGADEFSPTHFSNALTIPTYRDGDQLLARPFTGRLVISKTQNPRNIADGPFQHAHHTKEPFFVWLGERPEHSEHFNNYMSGYRQGKRRWMDEGFYPLKERLGADTPNDDKNPVLLVDVGGGLGHDLEELKVKHPGIRGRLVLQDQPEVIAQIGKASDGTELTTHDFFTPQSVKGSHQNWFELREKLTGRDLGARAYYLHSVLHDWDDASCLKILRNVVPAMRPGYSKLLINENVVPDVGAAWSITSMDWLMMALGAVRERTEKQWRGLLGQAGLKVTGVWTYEQGTESLIEAETET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGJ5</accession>
    <name>A0A8H6FGJ5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155381.1">
        <property type="nucleotide sequence ID" value="XM_037299380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGJ5"/>
    <dbReference type="GeneID" id="59336910"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034509423" evidence="1">
        <location>
            <begin position="20"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="283" mass="31321" checksum="F39E2A6BA103E006" modified="2022-01-19" version="1">MKLLQIRHLLALFLPIASTYKIESCDNSKPAVEYAITLAQTTMIRPLLDLQRGISSEHGYTAMYKSNVFKSFLQKLMTNILHLPVTQAAGRDQEPVFICAKAGMGQKYGIGYDPLDRCGQTGVTSFWAEDTVLVFLCPSFPNMRFQPSFTPGGPRDIYCPVVQNNVFLGRSDPLVKYQNYDLVHQLAHLYLQDGGLTGQTEPKEVMDWNSCVGLGWTPVEGGLSVKNPFNSVYYAAFVNQECTQMPDPFISPFSGQNDQTGLLSGGNITNALNTNGTDLRLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGP9</accession>
    <name>A0A8H6FGP9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U3 small nucleolar RNA-associated protein 13 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008680</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the ASTRA complex involved in chromatin remodeling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155546.1">
        <property type="nucleotide sequence ID" value="XM_037299545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGP9"/>
    <dbReference type="GeneID" id="59337075"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001009">
        <property type="entry name" value="Small nucleolar ribonucleoprotein complex subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013934">
        <property type="entry name" value="Utp13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF15">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08625">
        <property type="entry name" value="Utp13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="424"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="533"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="608"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="665"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="689"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 13 C-terminal" evidence="8">
        <location>
            <begin position="742"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="481"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6227238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="916" mass="98902" checksum="C8F40F7D47328C03" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGQ1</accession>
    <name>A0A8H6FGQ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155789.1">
        <property type="nucleotide sequence ID" value="XM_037299788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGQ1"/>
    <dbReference type="GeneID" id="59337320"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034401641" description="Apple domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="57426" checksum="AED939069BC353F2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH37</accession>
    <name>A0A8H6FH37_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription initiation factor TFIID subunit 1 histone acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008067</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037298935.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59336464"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040240">
        <property type="entry name" value="TAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022591">
        <property type="entry name" value="TAF1_HAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12157">
        <property type="entry name" value="DUF3591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 1 histone acetyltransferase" evidence="4">
        <location>
            <begin position="459"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="28"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="341"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="791"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="907"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1003"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="350"/>
            <end position="372"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="914"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="954"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1023"/>
            <end position="1056"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1125" mass="125938" checksum="92B8C8A8C70A8D98" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHF2</accession>
    <name>A0A8H6FHF2_9LECA</name>
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        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">HO133_008125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037298993.1"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="SUPFAM" id="SSF51430">
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    </dbReference>
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    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
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        <location>
            <begin position="6"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="332" mass="36707" checksum="87B17C68E6D34299" modified="2022-01-19" version="1">MSHVKVLFGTASFGSLPTEASQEFLDLLKKYNVKDLDTANRYVRVHLQISRTGADYMSPQEGSERALKELGATSSFTIHTKSPGFAKGSGTKASILAAAKQSFEDLGVNSVETYFVHSPDPYTPIEQTVDGMQLVYASGKYKHFGLSNFKPEDVRKVYDYAKSKGYVLPTVFQGNYNPVARHYDTTLFPLLRELNIAFYAYSPLAGGFLVKDAQTLNTGGGQGRWDPTSFLGKLYNEKYTKPSLLEALSEWDAIANEARVSKAALAYRWVMYNSKLSAEHGDGIIIGATRGSQLAETLRAIEDGPLDESIAKRIDKVWELVKDEAPVDNFNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHG6</accession>
    <name>A0A8H6FHG6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156225.1">
        <property type="nucleotide sequence ID" value="XM_037298889.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59336418"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037504">
        <property type="entry name" value="PSI_induc_2"/>
    </dbReference>
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        <property type="entry name" value="[PSI+] INDUCTION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40018:SF1">
        <property type="entry name" value="[PSI+] INDUCTION PROTEIN 2"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
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        <location>
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            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="311" mass="33606" checksum="3924B2CCAA739245" modified="2022-01-19" version="1">MVANSVHSQPYPMPLLLPRDGDPNFHSFSTVYEHGQVKSGWAFCMSKAYCKWPAIAGIVIGIIVFALAAYCCFRCCCRGGRRSRRNRAGTASMFNPAPYMGYQPANNPNNAPPPYGEPPRFAQFDVGGRGKAVNEDSLPAMPSWENAQKKHVEEDDVEMSRLEIPQEKNPMLAPGANASAADLSNPRFGYSEADSPLVGPYGAHHAQGQPPAGYTGPDFGHDAGHYTGPDFTSGIGQGQQTSYSAYTPSESTRYEPNGMNEPLELDTTYSNTLPPPSSTAQHTGFPPQHAPSVLQAGRRPGASSNDPWRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHM2</accession>
    <name>A0A8H6FHM2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6590 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007852</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156058.1">
        <property type="nucleotide sequence ID" value="XM_037298722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHM2"/>
    <dbReference type="GeneID" id="59336249"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046497">
        <property type="entry name" value="DUF6590"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20233">
        <property type="entry name" value="DUF6590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6590" evidence="2">
        <location>
            <begin position="87"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="261" mass="29668" checksum="5624142828978C82" modified="2022-01-19" version="1">MPGPPRVSYSNALSSKRRCPIAPPRGQPYSSIPRAPQNLGASRAQGMQPAKMQNATMSKVTEKFARMDLNWTKMKKQILDKDRCRVGMIIRAPLHEQDWQAGATTNSTGPKGDDFESGKSDITNDRSVSEWKHGYIHTKVRIFVVAAKFHRHFLAAPVYTHRHRGLRNKNRDYQEEHVSIFDKSHGPKNPERLQEGPHPYLYADLGDHAPHYKTMSTIHFTRLVPRGYGLECSIEGDLNAKSLPLFLNHCNDATQKFRATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI31</accession>
    <name>A0A8H6FI31_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_007039</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156569.1">
        <property type="nucleotide sequence ID" value="XM_037297934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI31"/>
    <dbReference type="GeneID" id="59335439"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000121">
        <property type="entry name" value="Serine/threonine-protein kinase nrc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000078">
        <property type="entry name" value="Serine/threonine-protein kinase nrc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="255"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="168"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="630"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6228927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="649" mass="70861" checksum="4087EB974FE4934A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI85</accession>
    <name>A0A8H6FI85_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA 4-demethylwyosine synthase (AdoMet-dependent)</fullName>
            <ecNumber evidence="4">4.1.3.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_007139</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TYW1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156667.1">
        <property type="nucleotide sequence ID" value="XM_037298032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI85"/>
    <dbReference type="GeneID" id="59335539"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="15">
        <location>
            <begin position="142"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="16">
        <location>
            <begin position="457"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="86"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="325"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="753"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="775"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6229025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="791" mass="88982" checksum="D756766393A7636F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FID7</accession>
    <name>A0A8H6FID7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156718.1">
        <property type="nucleotide sequence ID" value="XM_037298083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FID7"/>
    <dbReference type="GeneID" id="59335590"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034687938" evidence="1">
        <location>
            <begin position="23"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="244" mass="26552" checksum="F98CF527D02EE092" modified="2022-01-19" version="1">MLIKTLPSLNLMGVCLAPLALAYPSAEGSSDSNLLFKRAGTTAQLGDYTGSTSSYPDGSGTYVDSKDQGTYASGVKCWTDIFYVTNEYQAQAWTPMKENTIDCARTSTCASNSQDAVQNCGSWSITWGNEIEVDIIEKIGLELQENIDNTWLKSKCVTATVLNTCTWDDKLCHALWTSSVGQVFDGYIRRRCSGGRSGITSQPDYTAWSKDYSWTQPSPQTRLGCGALCDETTYPEPVPSVGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIG1</accession>
    <name>A0A8H6FIG1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156787.1">
        <property type="nucleotide sequence ID" value="XM_037298152.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59335659"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="897"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1246"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1424"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="904"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1451"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1463"/>
            <end position="1498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1579" mass="172939" checksum="F1FAC04E76C4B3F9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJQ6</accession>
    <name>A0A8H6FJQ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mannan endo-1,6-alpha-mannosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157098.1">
        <property type="nucleotide sequence ID" value="XM_037295097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJQ6"/>
    <dbReference type="GeneID" id="59332587"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053169">
        <property type="entry name" value="MUG_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47791:SF1">
        <property type="entry name" value="ENDO MANNANASE, GH76 FAMILY (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47791">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 191 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034995991" description="Mannan endo-1,6-alpha-mannosidase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="44689" checksum="A363A0E8C7AD8780" modified="2022-01-19" version="1">MQLGWPFLLFLYMYALSVLGSPAGVDGQVLTSYEPNVFHAHSAIIALQQWYNETTGLWETTGWWNSANALTMLADFAWVDSSLDVVAGHVFQNTFSRAQKTSLETIKVMTPYSVTTYTGPKFPPWIIPPKNGFAGFLNDFYDDEGWWGLAWLQVYDLTKQQQYLQAAIDIFEDMRKGWGGTCGGLWWNKQHSYKGAIENALFLTVAAQLANRASNKEYYLYWAWKEWDWFQRTGMINAHYNINNGINLVTCRNDNGIVWSYNQGVILGGLLELYTAAPNKTHLIMAERIALAGIKFLSDPSGILRDPCEPSCGADGPQFKGIFMRNLQKLQLAIPNNRFKTFINENANSIWLNDRGSGNQLGLLWSGPFMGATASTQSSAADALVAALAVGGQKVNLNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK37</accession>
    <name>A0A8H6FK37_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TBP-associated factor 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_004291</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157210.1">
        <property type="nucleotide sequence ID" value="XM_037295209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK37"/>
    <dbReference type="GeneID" id="59332699"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09839">
        <property type="entry name" value="M1_like_TAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000011">
        <property type="entry name" value="Transcription initiation factor TFIID subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="10">
        <location>
            <begin position="595"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="11">
        <location>
            <begin position="779"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1231"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1238"/>
            <end position="1251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6229953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1280" mass="144055" checksum="642090AE9AEEBB4A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKB0</accession>
    <name>A0A8H6FKB0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="3">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004416</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-1 OGG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157334.1">
        <property type="nucleotide sequence ID" value="XM_037295333.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332824"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902929">
        <property type="term" value="C:plasma membrane of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008534">
        <property type="term" value="F:oxidized purine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000005">
        <property type="entry name" value="N-glycosylase/DNA lyase OGG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024982">
        <property type="entry name" value="Rax2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048266">
        <property type="entry name" value="Rax2-like_second"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048265">
        <property type="entry name" value="Rax2-like_third"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778:SF2">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12768">
        <property type="entry name" value="Rax2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20842">
        <property type="entry name" value="Rax2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20843">
        <property type="entry name" value="Rax2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1553"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="domain" description="HhH-GPD" evidence="13">
        <location>
            <begin position="106"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6230077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1624" mass="171761" checksum="622E1E04CFEC32EF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKV4</accession>
    <name>A0A8H6FKV4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157663.1">
        <property type="nucleotide sequence ID" value="XM_037295662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKV4"/>
    <dbReference type="GeneID" id="59333154"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="497" mass="54737" checksum="FEED8B0438F670B4" modified="2022-01-19" version="1">MSFAAPVSKEGFWYNGDLYVEVGGLNRHKRAPVAEITAILRPDLKISKAATVQPSKDPVGHWYEAQLMHYGLPPSKDKARAKMRLLEAVNSSNLVVPLGISRLEESLRKEFNAAEKKAKAQYKLQSGGERKDAANPRKRKQPDSAPNVNVTVNFNGMDTMHFGEMPSPSVTHAPNPTEQQLHSSSKKAKATPKKDCKIREATIKTKAPQTASNKKALAAKDPITKKPSSTTEAVTAKQGKTGAQKEPTAKKQPAAKKEPPTKKEPTVQKESMAKKEPTVKKESMAKKEPTVKKESMAKKEPTVKKESMAKKTLTVKEKPAMKKETTTEKQRAVKAENLHSYPSASNVGSLGLINGLYNIECPTMREQFSDNFDPDSLSLILTLDGPRIWGAYDFGQYSGVLLLENRPYSASNEALPVKWRGRENGEGEMSFGDHCYGDIAFHGNGRITGMLNFYGDCEFSGTRQPGPGTALRPAASMKEEWGGYNDSSYSYEASNRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKV9</accession>
    <name>A0A8H6FKV9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA-processing protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004746</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157661.1">
        <property type="nucleotide sequence ID" value="XM_037295660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKV9"/>
    <dbReference type="GeneID" id="59333152"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="356" mass="38012" checksum="B2602842B3F32625" modified="2022-01-19" version="1">MAPPPTPLSPSELSYLHTSLSLHPPIRPDARSPTSFRPLVAETDLLPAANGSARLCFADGTEAIAGVKAEVEKVQGLGNVRWYDNMDAEMGGIEESERDGEQEGERGKGVGEDKWIEVTVDMPGQRDDDAMVVFLAQMIHEGLVADGGLKNRLVINARWHWRLYIDILLLSPPHTYPLPLLSLTTHLALLSTRLPAPISNHDEDPLFNDDWDASSPLYPSSDTYPDAKTPPQSSPARPPITLLVISVGDNVFFDASREELAVADAVLAVTVGAPSSPSTGLNLLAVRTIDPPSRLSASAAEVGRAGAEIDAEGGKEGVWKASKGGMKRRVLARMVQMCVEGGGVGEEVLGGLGEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL02</accession>
    <name>A0A8H6FL02_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-(3-amino-3-carboxypropyl)histidine synthase subunit 1</fullName>
            <ecNumber evidence="4">2.5.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diphthamide biosynthesis protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphtheria toxin resistance protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_004814</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DPH1/DPH2 family. DPH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157728.1">
        <property type="nucleotide sequence ID" value="XM_037295727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL02"/>
    <dbReference type="GeneID" id="59333220"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11850:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6230471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="450" mass="50124" checksum="1930BC342F1CF6E3" modified="2022-01-19" version="1">MEDDRAQADLGEAIDAELGAEKQEKQKYPRKRFIGRKEAAERAEKRGDANGTIEDSGAIQVAQPRKTARALNQVPSEILNDLEINDAISLLPPNYSFEIHKTIHRIRTSGSKKIALQFPEGLLLFATTISDILTRFCPGTETLIMGDVTYGACCIDDYTARALGCDILIHYAHSCLIPVDVTKIKTLYVFVDISIDTSHLLATLERNFQPGKTIAMVGTIQFNATLHTVKPVLERAGFKVSIPQITPLSRGEILGCTSPRLSTDQADLIVYLGDGRFHLESAMIHNPSLPAYRYDPYSRKLTRETYDHEEMHDLRRQAIKSAKKAKQWGLILGSLGRQGNPHTMTMIEHTLDERGIPFINLLLSEIFPGKLAMMNDVDCWVQIACPRLSIDWGYAFPKPLLTPYEALVALEAREDWGHGNQGVYPMDFYAKDGLGRTKEGDVGPMVAAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZI7</accession>
    <name>A0A8H6KZI7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nineteen complex-related protein 2-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004244</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157164.1">
        <property type="nucleotide sequence ID" value="XM_037295163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZI7"/>
    <dbReference type="GeneID" id="59332653"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012890">
        <property type="entry name" value="GCFC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028211">
        <property type="entry name" value="Ntr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214">
        <property type="entry name" value="GC-RICH SEQUENCE DNA-BINDING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214:SF0">
        <property type="entry name" value="LD29489P"/>
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        <source>
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    <sequence length="491" mass="53959" checksum="8421C2CB7EECFD67" modified="2022-01-19" version="1">MRSSFGARRQARKVGQQEGEDMEDARPGLGSQDQENTPTVIRPSIASRGSSKTKKQSSARVSFGPGGTSMTEDDDIGGPAVFTPKKSSLSRQAVEKNALRKTIANNLSSEHLGFRQTEDRPSYSADHLNELKTSTPSTPKNLRSKSDVNDEDGKALDLASKFGSDLAYQESSSAIPTDAEVREKKARRARLAKEQDFIGLSDEDAQEQEDSDGERSLLPYAKQKPSKKEETRLVRDDEDIAEGFDEFVSDGRIALGKKAEREQKQRQEAEMRNLINEAEGGNDSADSEDDDSEAERHAAYEAAQTRKGMDGLRRDEQSTRPRRPRTPPRITPLPTLAGCLEKLRQRLAGIEYARMQKVRQLEEIKKEKEDIRVREGEIQQLLKETGERYEKLRKEAEMSGEDRPPDGLSPGGGLGSGASMVSMAGIDPAQQTPETKAPPPTSDSPPAPAGASQPLLASRAKGNRRASPTYAEQRGKPADTSSPKHFDTLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="916" mass="103166" checksum="12B889134478B0AA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
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                <person name="Walston R.F."/>
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            <dbReference type="PubMed" id="32504651"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="match status" value="6"/>
    </dbReference>
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    </dbReference>
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            <end position="1213"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6217487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1213" mass="134792" checksum="489680B029C6866C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5J2</accession>
    <name>A0A8H6C5J2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DsDNA-dependent ATPase</fullName>
        </recommendedName>
    </protein>
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    </gene>
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            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037297557.1"/>
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    <dbReference type="GeneID" id="59335061"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18004">
        <property type="entry name" value="DEXHc_RAD54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000035">
        <property type="entry name" value="DsDNA-dependent ATPase (Rad54b)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="296"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="624"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6217558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="925" mass="102187" checksum="4ED3593F52AECC0A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6B9</accession>
    <name>A0A8H6C6B9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147203.1">
        <property type="nucleotide sequence ID" value="XM_037297493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6B9"/>
    <dbReference type="GeneID" id="59334996"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="82" mass="8515" checksum="E6031A11074E0D13" modified="2022-01-19" version="1">MPSLPSLTSSDKPSTSDTSIDKESVTKNVSSAMDDANEKSGSSAAAETKAGVAQTASEVEKAANKLYEENIEDEYAKREGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6V2</accession>
    <name>A0A8H6C6V2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147488.1">
        <property type="nucleotide sequence ID" value="XM_037297363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6V2"/>
    <dbReference type="GeneID" id="59334866"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42041">
        <property type="entry name" value="DNA ENDONUCLEASE ACTIVATOR CTP1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42041:SF1">
        <property type="entry name" value="DNA ENDONUCLEASE ACTIVATOR CTP1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="545" mass="61543" checksum="11E2CE5E8544F272" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C762</accession>
    <name>A0A8H6C762_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147638.1">
        <property type="nucleotide sequence ID" value="XM_037297064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C762"/>
    <dbReference type="GeneID" id="59334566"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="562" mass="63404" checksum="09043C20CDB323CD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C862</accession>
    <name>A0A8H6C862_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Lysophospholipase</fullName>
            <ecNumber evidence="2 9">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005810</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the lysophospholipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 9"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147896.1">
        <property type="nucleotide sequence ID" value="XM_037296715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C862"/>
    <dbReference type="GeneID" id="59334215"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034862503" description="Lysophospholipase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="10">
        <location>
            <begin position="30"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="534" mass="55648" checksum="BBF49BACACC73D45" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8F2</accession>
    <name>A0A8H6C8F2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RlpA-like protein double-psi beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005345</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148237.1">
        <property type="nucleotide sequence ID" value="XM_037296255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8F2"/>
    <dbReference type="GeneID" id="59333751"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF28">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034730389" description="RlpA-like protein double-psi beta-barrel domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="27475" checksum="FA5AC8B10F4E6533" modified="2022-01-19" version="1">MKLPAVALVLAISIPLANAYTGDLTHYTPGMGSCGIVNKNGDAIVALSADIMLSFSGNANDNPLCNKQISIHNPTTGTNTIATVTDTCQGCAFDNIDVPDDLFYTVAPEGNGRVPGIEWTPVGWTVPGGSGDSESTAAQSGVSSVVQPSSVAAAIPSAALPSIHLQEKVAAVVPSAAAAAPSGSSSTSAAPSSVSAVAASVAPSAASPSNTQTASAPTDSITSCTTAGETVCSADGTQIGTCTMELTVQLGRVAQGTKCVGGYMVMANSRLRSRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8Q0</accession>
    <name>A0A8H6C8Q0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148493.1">
        <property type="nucleotide sequence ID" value="XM_037296511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8Q0"/>
    <dbReference type="GeneID" id="59334008"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF5">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="155"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="37750" checksum="70B604845B76ADCA" modified="2022-01-19" version="1">MSIGIAIIGAGIFARQEHLPAVQATPLLSLKAIYSRSKASADKLAKSAAKPVKTYYDSPATSGHSLDDLLAHQDIDAVIIALPICVQPTVIKAAIEAGKHVLSEKPIAKDIETAAHLIRWYKHQRREETWSVGENFRFFEPIVFAVEQIKKLGGELVTFSVDVHAYIDENDEYFQTAWRQKPDYQGGFLLDGGIHFVAALRCLLEAAGQSISIVRASTSLIKEDLGPLDTLNANMTTTDKRNGTFNLSYGSKFRRDCRVCIVTTRGAVAVSPSEVTVSTGDKTTTQNKTFRFPPGSGVRKEVAAFAESISAKEPNPRGSPEQAYLDLKLLQGMLESGEEEGAAKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9A4</accession>
    <name>A0A8H6C9A4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005034</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148644.1">
        <property type="nucleotide sequence ID" value="XM_037295946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9A4"/>
    <dbReference type="GeneID" id="59333440"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="32"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="333"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="482" mass="52765" checksum="389A92F793AB79DE" modified="2022-01-19" version="1">MSQAKQLAQYHNSGVDGHGFGSPNDAARRQVACDRCRGQKLRCIWDENVGQCRRCAKANVVCMILPPRPMGRPSRPTHRSDSQSKSSYEHIPLQDPQWAAFMQSQSLHAGSCAAMEQDDIDMQDTLTEGTRTDFDGTNRGATARSSIDHLLNGQAAHFSSELFPHSDMLHTNENLQDLDDDSFLVHSSLMQNANNTYKIPTFGSDKTFVPSRCHNPTMNINRPAAPQFIPPDANSEGDENTDEDLDDRSLCTITKLLRLNLALYKHLTQMNAGQRESPAPVMTPVSPESPSAPSPKPAMVPRMMIGRLLSLTDEFSTLLGQLGLLARTHQIPGSGSDTASPYGSVSSTTTSGNASRCSRSRSSPEASQWKQHDPCTALLVLSCYLHLKQVHARSRLALNQLLSRDVEPEDLPELLPGLVIEGFSLAGHGRLQLGMTAKLCEQMFNSIGPCMGLDDSVYDAAPSGLDELVEKIWGLFGREKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9D2</accession>
    <name>A0A8H6C9D2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005613</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148504.1">
        <property type="nucleotide sequence ID" value="XM_037296522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9D2"/>
    <dbReference type="GeneID" id="59334019"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01299">
        <property type="entry name" value="Met_dep_hydrolase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051781">
        <property type="entry name" value="Metallo-dep_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057744">
        <property type="entry name" value="OTAase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135:SF3">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="87"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="530" mass="56757" checksum="23D4AD385E273E6B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9E0</accession>
    <name>A0A8H6C9E0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005172</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <topology evidence="1 9">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148782.1">
        <property type="nucleotide sequence ID" value="XM_037296084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9E0"/>
    <dbReference type="GeneID" id="59333578"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF2">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 9" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034945577" description="1,3-beta-glucanosyltransferase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="11">
        <location>
            <begin position="382"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="483"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="547" mass="57732" checksum="E7DF90C0D02EA7A6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9I8</accession>
    <name>A0A8H6C9I8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148779.1">
        <property type="nucleotide sequence ID" value="XM_037296081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9I8"/>
    <dbReference type="GeneID" id="59333575"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15457">
        <property type="entry name" value="NADAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.40">
        <property type="entry name" value="YbiA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012816">
        <property type="entry name" value="NADAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037238">
        <property type="entry name" value="YbiA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08719">
        <property type="entry name" value="NADAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143990">
        <property type="entry name" value="YbiA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADAR" evidence="1">
        <location>
            <begin position="8"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="84" mass="9078" checksum="3BC2C9D478A7E504" modified="2022-01-19" version="1">MAQGPRWALLMSDDAVAEKILVGSGPAEAKALSREMSNFDQEVWDANCNGNVEEGNLLKFQRNENLKDVLLAPPGLGMCGNESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9P6</accession>
    <name>A0A8H6C9P6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <ecNumber evidence="6">5.4.2.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cofactor-independent phosphoglycerate mutase homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_005059</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BPG-independent phosphoglycerate mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148669.1">
        <property type="nucleotide sequence ID" value="XM_037295971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9P6"/>
    <dbReference type="GeneID" id="59333465"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006007">
        <property type="term" value="P:glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16010">
        <property type="entry name" value="iPGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1450.10:FF:000001">
        <property type="entry name" value="2,3-bisphosphoglycerate-independent phosphoglycerate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000001">
        <property type="entry name" value="2,3-bisphosphoglycerate-independent phosphoglycerate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1450.10">
        <property type="entry name" value="BPG-independent phosphoglycerate mutase, domain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01038">
        <property type="entry name" value="GpmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011258">
        <property type="entry name" value="BPG-indep_PGM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036646">
        <property type="entry name" value="PGAM_B_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005995">
        <property type="entry name" value="Pgm_bpd_ind"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01307">
        <property type="entry name" value="pgm_bpd_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637">
        <property type="entry name" value="2,3-BISPHOSPHOGLYCERATE-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637:SF0">
        <property type="entry name" value="2,3-BISPHOSPHOGLYCERATE-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06415">
        <property type="entry name" value="iPGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001492">
        <property type="entry name" value="IPGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64158">
        <property type="entry name" value="2,3-Bisphosphoglycerate-independent phosphoglycerate mutase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="16">
        <location>
            <begin position="6"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="BPG-independent PGAM N-terminal" evidence="17">
        <location>
            <begin position="92"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="13">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="163"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="270"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF06415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6219234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="522" mass="57422" checksum="17F0F90070BF0E4D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9V9</accession>
    <name>A0A8H6C9V9_9LECA</name>
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        <recommendedName>
            <fullName evidence="8">Rad21/Rec8-like protein C-terminal eukaryotic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149017.1">
        <property type="nucleotide sequence ID" value="XM_037294970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9V9"/>
    <dbReference type="GeneID" id="59332460"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030892">
        <property type="term" value="C:mitotic cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21789">
        <property type="entry name" value="Rad21_Rec8_M_SpRec8p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF70">
        <property type="entry name" value="RAD21_REC8 N TERMINAL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G02900)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="5">
        <location>
            <begin position="38"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="4">
        <location>
            <begin position="641"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="279"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="405"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="503"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="416"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="683" mass="71771" checksum="3BD9DBE758918A17" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA78</accession>
    <name>A0A8H6CA78_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149154.1">
        <property type="nucleotide sequence ID" value="XM_037294466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA78"/>
    <dbReference type="GeneID" id="59331955"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="824" mass="91581" checksum="4071B238C2507B85" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAV6</accession>
    <name>A0A8H6CAV6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003195</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149499.1">
        <property type="nucleotide sequence ID" value="XM_037294118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAV6"/>
    <dbReference type="GeneID" id="59331606"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="7">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="120"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="196" mass="21595" checksum="6B988650C4A764C9" modified="2022-01-19" version="1">MLRQAQLKSGQLFLVDLAGSEKVGKTGASGQTLEEAKKINKSLSAGNDGKSTHIPYRDSKLTRILQESLGGNSRTTLIINCSPSSYNDQETLSTLRFGVRAKAIKNKAKINAELNCKKLAGQETPSRSESRTGDRASTPSIVLEKDEREEFLRRENELQDQVAEKESQLSTAEQALKEAKGELQFLKENKALEQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBH5</accession>
    <name>A0A8H6CBH5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002948</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149950.1">
        <property type="nucleotide sequence ID" value="XM_037293872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBH5"/>
    <dbReference type="GeneID" id="59331360"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012332">
        <property type="entry name" value="Autotransporter_pectin_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039903">
        <property type="entry name" value="Zswim2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540:SF0">
        <property type="entry name" value="PHD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540">
        <property type="entry name" value="RING FINGER AND SWIM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69349">
        <property type="entry name" value="Phage fibre proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="419"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="323"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="152"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="473" mass="52072" checksum="70A7A15023E4D3FA" modified="2022-01-19" version="1">MPTPQWEPAEVLRLDPYSSGFTCIGYAPSKGRRCRNPIACANRQDSAKILLEMSRLDPQSQRVDDELEDLASRLLCRRWHQDQALDMKRQWRRNIESYRAAETARLRERTEYLVQLLFTAAEVRAERSSTVRAERSSTVQAERSSPVRAERSSAVQAQQSSTVQAERSSAVRAEQSSTVRAERSSAVQAQQSSTVQAQQSSTVQAERSSAVRAEQSSTTQVEHSSIVRAERSSTVQAERSSAVRAEQSSTVRAEQSSTTQVEHSSIVRAERSSIVQAERPSTVRTTPSLAPSTLAQTRATTLRIDQTATSVVHLSIHVSSSATLSITTGEEPGRRENNDSNEGESRRQTDPDPNSSSPPEALPQETNQPETNPSPIPSAANLTTPREEAANATSSEAPIPEPPTPSHHSHDRRPIQGDCSICFEDLSSGGDTVWCRAQCRQNLHAECASLWLASQEAIGRVKTCPYCRAQWAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBI7</accession>
    <name>A0A8H6CBI7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002931</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149933.1">
        <property type="nucleotide sequence ID" value="XM_037293855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBI7"/>
    <dbReference type="GeneID" id="59331343"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092:SF2">
        <property type="entry name" value="HERCYNYLCYSTEINE SULFOXIDE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092">
        <property type="entry name" value="L-CYSTEINE DESULFHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="72"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="450" mass="50870" checksum="0102D20BD5249BCF" modified="2022-01-19" version="1">MADALTPITSRCQFGRTMRDTHFMLAPTYTPLNHGSFGTYPKPVQKRLHECQTLSEARPDNFQRYDYPVMLDRSRAAMASFLNVPVDEVVFVPNATTAINVVLENLRFKKGEVILHLSTLYGSVAKMIEYIHETRGVESVNVAVEYPIDDDVLVSRFQTAINDVKVGKRRRVGVAIFDTISSLPGVRVPWERLVEVCREENVLSLVDGAHGVGQIDLGLSKVQPDFFTSNCHKWLYVPRACAVFHVPRRNQHLIRTSLPTSHGFVPRPRGPGHEIMNPLMKDASSTSPFALLFEFVATLDVSPYLCIEEALRFRREVCGGEEKIMRYCEEISSEAGRQAAKILGTGVMENGTKMLTKCAFTNVRLPLEIGDGREQIPEKDMYLVAVWMTAMLVRESDIYSPVFFHARRFWTRLSGQVYLDLGDFEKGARALKTLCERAKSGEYLQQKARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD30</accession>
    <name>A0A8H6CD30_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Monocarboxylate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002122</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150702.1">
        <property type="nucleotide sequence ID" value="XM_037293049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD30"/>
    <dbReference type="GeneID" id="59330536"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF156">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="50233" checksum="B891E6D71DDE2BEF" modified="2022-01-19" version="1">MTPNERISLRESLPSGVGEQEPHRTEQDASNLDQADSRLWESNRKRACVLAGSAILQLPIWGFAMNYGVFQEYYSSNWTLQGSHQVTGIIGTTSNGVMYLSMPFLFALFTRRWARRRQTAALCGAALTCISFLLSSFSTHVWHLVATQGVMAALGCALIYSPTTLSLGEWFNTSNRAVAYGVVLSCKNIVGSACPFLLRVLLDRYGFRITLRIWAAVAAGTSILAIFLIPTHPSSISSSTHRTRKIPWQFLKHQTFYIYSVAIILQSSGYGIPQTYLNTYAHEVTLLSQTSATLLLTLFNIPGIVASSFFGYLSDNTRFPLSATTVTSISAVSSALSAFLLWGLTSQGSMALLVLFSITFGFFAGGYSATWGGVINELEREAAQRNEAVDTGMLYGLLNGARGIGYVSGGLAGVPLLKAGSTSAVGSFGYGTTYGPLIIFTGLSSVFGGWGLLWKWKKLLHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE20</accession>
    <name>A0A8H6CE20_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001646</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151113.1">
        <property type="nucleotide sequence ID" value="XM_037292574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE20"/>
    <dbReference type="GeneID" id="59330060"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39606:SF1">
        <property type="entry name" value="CELL SURFACE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39606">
        <property type="entry name" value="SURFACE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="371" mass="39019" checksum="BFBEE8841C482E31" modified="2022-01-19" version="1">MPDSKDKKSRFGLFGHKDRPTPTTDSTYGSEASSDAHNSYQSAPSMTGSDNTGTTVNDQGQTVTTTTTTTTTTTTGGGGASTTSGPHTSNLANKLDPRVNSGGDQTEVSETVQRRENDRPNIPSKSTRREPSPNPPPQQRSSGGGGGLSSILSGGKNHEQRQPDNYGNQNPPPQQQQSTGGGGLSSILSGGRNHDQRQPDNYGSSNAPPQQQSSGGGGLSSILTGGRNHHDQRQSDNYGSPSSPQGQGRHNFSYPSRTPPNERAQLPAQTQGQAPSQQQQQPPSTLQSLKTAAVGIHGVGETLRGTLNSSVDRRFHAGPEQQAHNERVLNAGRQEIESGHLSENSRQQGGPGTKGILRKSGGQGQLRVVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE51</accession>
    <name>A0A8H6CE51_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001770</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151237.1">
        <property type="nucleotide sequence ID" value="XM_037292698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE51"/>
    <dbReference type="GeneID" id="59330184"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000002">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="16172" checksum="3E38F64DD7BDA592" modified="2022-01-19" version="1">MPPKKKVERAATENISLGPQVREGELVFGVARIFASFNDTFVHVTDLSGRETISRVTGGMKVKADRDESSPYAAMLAAQDVATRCKELGINALHIKIRATGGNGTKTPGPGAQSALRALARSGMKIGRIEDVTPTPSDSTRRKGGRRGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEB1</accession>
    <name>A0A8H6CEB1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151099.1">
        <property type="nucleotide sequence ID" value="XM_037292560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEB1"/>
    <dbReference type="GeneID" id="59330046"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="432" mass="48375" checksum="67F3A3358607D587" modified="2022-01-19" version="1">MDIKDHNANGLGNETPYVARGPARASHDEDSASYIKPIIKELVRTQVCFPNIKLLVDKISTVKVPSATESSGLEAYRMHLTDREKTIQAVVKRRLHKWLTTFNVREGSYVVLKEYNLARGKRLGGEGDVFYLAISDFYSIGEEQRYNRSTDPKSVSASAEEGDSGPQDQVSAKDEDQSNEVDIIRTERSLPRASPTETHEDIASSKDLFFAKSAMAELTKRKRGMAPPDLDPDLISLASKAQRLRETPLAKMTREKEHDNAQPRSTDDHCSSASTPNIPITRPQSAQIRASPEQVPSPLQLSTLASVTGTNATRNKQVNILAMIEYVSNSTVKPTTAPLKRDVRIVDASTDKKVTLSVFVDPVNFIPKEYDIMLFRDLTTHDFSGGNLNAYPKKCRGKEWYILNPYDTEECDMKSMEVFRTKYRKTKAQGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEH1</accession>
    <name>A0A8H6CEH1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001191</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151540.1">
        <property type="nucleotide sequence ID" value="XM_037292121.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59329607"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23827">
        <property type="entry name" value="RWD_YLR419W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000016">
        <property type="entry name" value="ATP-dependent RNA helicase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000868">
        <property type="entry name" value="DEAD/DEAH box helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001214">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056328">
        <property type="entry name" value="DSRM_DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF267">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE YLR419W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24385">
        <property type="entry name" value="DSRM_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="359"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="RWD" evidence="8">
        <location>
            <begin position="433"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="614"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="853"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="222"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1371" mass="152035" checksum="4CA2424375216EBD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEH8</accession>
    <name>A0A8H6CEH8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Azaphilone pigments biosynthesis cluster protein L N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151422.1">
        <property type="nucleotide sequence ID" value="XM_037292883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEH8"/>
    <dbReference type="GeneID" id="59330369"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031348">
        <property type="entry name" value="PigL_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17111">
        <property type="entry name" value="PigL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Azaphilone pigments biosynthesis cluster protein L N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="496" mass="56055" checksum="6F76015BF1EBD19D" modified="2022-01-19" version="1">MPDPLTALSTAASVVSLVDVTIRACEGIYGLVISWRNAPRAVKSLRQTVEGIESILRSLKTFVTEYESSVAFRQHRQVLPDSVNADIISIGETLGLLDGILTSSGETKNVRRRAKWAIDEKKTLEIVQRLETQQVALILSLQTVAQRSSIARHHDMFRIETNLKQSHPISTEQVLNALKPISRRLDVIAQNEASASMGHASLSTSIEALRETNLDLHNTLDGKLDNLMSGLSQAPFLRDSIRSEIFVRPSTNDVLARIFREELKHVVIPIVEDRLKANKSASEAQIVSIRESIDSMASEFQQNLNYEDSDELITFLRCQVLEIDDVKADELAEQLRHCLNVTPFFKSAGIGYGFYATQRTYYLCGLGANVRARDASGRQPLHYLFQKPWSDETSCHVIYLAYLLIKTGADIWACDDRGNTPTSLVLKHDRWDDWCKILERCGLDVEHVLRTERDNRWDRQHLNDAERSSVDTEDLEFPGHEVSLRRRGAYTREVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF93</accession>
    <name>A0A8H6CF93_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM complex subunit LSM3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM3</name>
        <name evidence="11" type="ORF">HO133_001381</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF93"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01730">
        <property type="entry name" value="LSm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000036">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034105">
        <property type="entry name" value="Lsm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040002">
        <property type="entry name" value="Sm-like_LSM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13110">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="14"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6222295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="98" mass="10849" checksum="C41C6A0A3ED8E71B" modified="2022-01-19" version="1">MADAGDGDTAAVSEPLDLVRLSLDEIVFVRLRGDRELKGRLHAYDSHCNLVLGEVEETIYVVEDDDEEEETVKTIKKQSEMLFVRGDSVVLISPQAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGB4</accession>
    <name>A0A8H6CGB4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_000723</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152022.1">
        <property type="nucleotide sequence ID" value="XM_037291660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGB4"/>
    <dbReference type="GeneID" id="59329141"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000006">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000004">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000052">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="17">
        <location>
            <begin position="297"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6222676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="600" mass="67078" checksum="B6A063F77EE7AC47" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGN5</accession>
    <name>A0A8H6CGN5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IgE-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152376.1">
        <property type="nucleotide sequence ID" value="XM_037292014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGN5"/>
    <dbReference type="GeneID" id="59329500"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052820">
        <property type="entry name" value="PhiA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42047">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42047:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034833993" description="IgE-binding protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="20016" checksum="24BF7AECB4B8F793" modified="2022-01-19" version="1">MYPIVVLALLPFLSSAIPVSPLQSNSSTPFVLRALRLGFPIHLSTLNANDGQFWLGKATSSSCPAEPKTDCPTETETAVVDTHVPFGQSIYVAKDGTLSFTAAHEEEKVVAASTAYPAGFAYDRDARSFTFAGTPSKGWLACPVAAESGDGDGEGPWLVYADLKRGGKTGCLAFEAGGVEYGGVAVWEYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGY9</accession>
    <name>A0A8H6CGY9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc-binding loop region of homing endonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152375.1">
        <property type="nucleotide sequence ID" value="XM_037292013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGY9"/>
    <dbReference type="GeneID" id="59329499"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.75.10">
        <property type="entry name" value="Homing Intron 3 (I-ppo) Encoded Endonuclease, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044930">
        <property type="entry name" value="Homing_endonuclease_His-Me"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="56488" checksum="DF0F190A1EF10128" modified="2022-01-19" version="1">MFRQPFVSIPGSFDGSSGTLKRKLVGEDFGSATTFNSTIGKRKRSEYNEVPHLPSTQYRKSEELLNVRVLDDPVWPATTAIYVSGHETQALSDSGKFGVLPSDREANGCYKKSQLDLRRSWHDPPAHYTADEDSDSATRKTTRNLKTDSMTVVERKHFFIHTPTEGMKAATKKYLSDIYQLLDMSGSNDDCRLHPTPPQFNGKPVRAISFGFNWSDDHGFHRLTVDWGIVALVVRQQLTDDQMDGFINKSWHLSHLCGNWTCCNWRHFTVESGPINCGRNGCFNSPVKCTHNPPCMKEKKRQLLVTDNIRSEISKAITSLGGILSYEAFHALAEYDIRLVECFWENSKRGFCAFCGRSDDKAHICSSLSSLVDCKVMLRALKQCVKPTSEVREAIGYLVKIREDLERGSTVKDKILTEWLFSPGRCTELEQTSPRLKKTRRELRKKRLEVPSKCRQPGSTAENVDQLDEVENPHLKSLQATRSGLQELKKFRKVQGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHQ1</accession>
    <name>A0A8H6CHQ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2423 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000294</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152668.1">
        <property type="nucleotide sequence ID" value="XM_037291233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHQ1"/>
    <dbReference type="GeneID" id="59328713"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019434">
        <property type="entry name" value="DUF2423"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219:SF1">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10338">
        <property type="entry name" value="YBL028C_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2423" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="140" mass="15742" checksum="F8CEE5FCAB0BB2C6" modified="2022-01-19" version="1">MAKGLRSSRNKANKSKLRSNVFGPAEIARKERLSAKLIELASKIQSNPEEDLKMVDREKVTEQEIAKEPEAHVPASEDEMDNDQAKQPSSTTELVSRSTSRVQKRGRGKARASMVFPIYKKGKRVGPRLSARDRKKSLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHQ9</accession>
    <name>A0A8H6CHQ9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152772.1">
        <property type="nucleotide sequence ID" value="XM_037291337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHQ9"/>
    <dbReference type="GeneID" id="59328817"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="277" mass="30275" checksum="8167E101FD9A5F8E" modified="2022-01-19" version="1">MAKVDDGVVAVTSVNDQDGLPMHNQAQRHSSLSRIEAYRYHNTTRTTDPGGGKVTNSGIAVPSVNNQDGILIDYYTQYNGNGSTDDGWPAKDKWISFMDMFNNSRWHMERSCHQFNVDLNTHQEIIDMYEAIQFVAAATFVDHRFILAVIMQESGGCVRAPTSNYGVPNPGIMQDHNGTASCNNGKTQKPCPGETITQMVQEGTGGTTWGDGLAQCLNQAGTSDVSAFYRAARKYNSGSIDDSGDLGKGIATHCYASDIANRLTGWVQYPKNCTLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHU0</accession>
    <name>A0A8H6CHU0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000531</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152905.1">
        <property type="nucleotide sequence ID" value="XM_037291470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHU0"/>
    <dbReference type="GeneID" id="59328950"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Ctr9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027:SF2">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="258"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="804"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="960"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1016"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="963"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1018"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1045"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1100"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1165"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1189"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1249"/>
            <end position="1265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1265" mass="141560" checksum="9240072F3F99E84E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIR8</accession>
    <name>A0A8H6CIR8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="9">2.7.7.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CTP:phosphocholine cytidylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphorylcholine transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_010699</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153185.1">
        <property type="nucleotide sequence ID" value="XM_037301551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIR8"/>
    <dbReference type="GeneID" id="59339089"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000147">
        <property type="entry name" value="Cholinephosphate cytidylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF13">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="161"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="353"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="445"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6224125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="498" mass="54159" checksum="4E7C50447562F064" modified="2022-01-19" version="1">MSSPSSSGSAKRKRTSTLQSSTKQSSTADLLQPSSRDASGEDAADSASQAVTTIKHKKSAASADSTGASNKRPRTRSNAADGPTSNGAIPSINSADAEDSENTVEEGGRTKRRTKSSSNKQRPDLNTADRKAMPPPAKGGLQDPVGFHTNPPPTGRPVRIYADGVFDLFHLGHMRQLEQAKNAFPDVYLMVGVTGDAETHKRKGLTVLSGAERAETLRHCKWVDEVIPNCPWIVTPDFLEKHKIDYVAHDDLPYGADEGDDIYTPIKREGKFLVTQRTEGVSTTGIITKIVRDYEKYIARQFKRGTSRQELNVSWLKKNELDLKRHAMELRDSVRNNWSTTGQELTKELRQFWQSSRPGSPARGLGRNGSDADLDRSGGQKSSPAALAHLAHLDIPRPESPGGGRGASSDFATGYSLGLIGGVRSWVGRKVALHVEWSLKITQMTRSRTSLRGSQPDSGESEDDSSDAKSPKEEKTPSRGRRGEPSNAEVADMMDTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIS9</accession>
    <name>A0A8H6CIS9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153339.1">
        <property type="nucleotide sequence ID" value="XM_037301705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIS9"/>
    <dbReference type="GeneID" id="59339244"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="199" mass="21758" checksum="C60D8574015EF94A" modified="2022-01-19" version="1">MLPSPIDPIPDRAFGLKATIDFELPDQFWSLTAPNSTNSTKKQAATPPPQPATDPPANHKPIAAPSTMADGALGCDSSYAQLPVHCDAAYYEKWNRERQEAVARNEDLNAKFLTLVAQREANSASGQLRRLFKKGSKTYGQGLTAGEEQKAWEDSMKKGVGKRGGGGKGDEVVRRLRDVEGEAILDEMTGWKSTHTRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ19</accession>
    <name>A0A8H6CJ19_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010630</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153116.1">
        <property type="nucleotide sequence ID" value="XM_037301482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ19"/>
    <dbReference type="GeneID" id="59339020"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000776">
        <property type="entry name" value="Guanylate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF13">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="200" mass="22227" checksum="E411551719156FD2" modified="2022-01-19" version="1">MAALATQIHRPIVISGPSGSGKSTILKRLFADHPDTFGFSVSHTTRSPRPGEQDGREYNFTNQEAFLALVRDGGFIEHAKFGGNYYGTSVEAVNDVAEKGRICVLDIEMEGVKQVKKTDLGARFLFLSPPSLKILEERLRGRGTDDEESVKKRLEQADKEMAFAKEKGVHDRIIVNDDLNRAYQEVEEWVVDGGRFGGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJM5</accession>
    <name>A0A8H6CJM5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153614.1">
        <property type="nucleotide sequence ID" value="XM_037300797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJM5"/>
    <dbReference type="GeneID" id="59338333"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="213" mass="23447" checksum="86B29E759556DC74" modified="2022-01-19" version="1">MAGGETPDPASAFRRVLPWRVFDLQSDPQRSHRTRSLAGETISVTSTLAALIWVVVYLSGAMTKEAVGCTARLMPENTVTLGGESALIDKLCVTAVNGIVEGAERRPHRRVHSVTPESPKPRFTKEAEIPKGGDLDGHEVGRDPIAESPGRGLRARQSTVPRNKKLPIDGLVPWLVQRIGRYKNGVNAPLHGRMMMDEVEVGGGQLRRRQWYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJP4</accession>
    <name>A0A8H6CJP4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 58</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_011016</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for pre-18S rRNA processing. May bind microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153499.1">
        <property type="nucleotide sequence ID" value="XM_037301865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJP4"/>
    <dbReference type="GeneID" id="59339406"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000003">
        <property type="entry name" value="Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000001">
        <property type="entry name" value="Probable Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="285"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="449"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="704"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="489"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="504"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="545"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="559"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="592"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="607"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="620"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="708"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="744"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6224439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="756" mass="82923" checksum="831114A550BEB8D4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK24</accession>
    <name>A0A8H6CK24_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase C45 hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010152</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153824.1">
        <property type="nucleotide sequence ID" value="XM_037301007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK24"/>
    <dbReference type="GeneID" id="59338544"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.60.10">
        <property type="entry name" value="Penicillin V Acylase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047794">
        <property type="entry name" value="C45_proenzyme-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047801">
        <property type="entry name" value="Peptidase_C45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005079">
        <property type="entry name" value="Peptidase_C45_hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040521">
        <property type="entry name" value="C45_proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34180:SF1">
        <property type="entry name" value="BETA-ALANYL-DOPAMINE_CARCININE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34180">
        <property type="entry name" value="PEPTIDASE C45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03417">
        <property type="entry name" value="AAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C45 hydrolase" evidence="2">
        <location>
            <begin position="127"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="367" mass="40347" checksum="B1EF5619D809C299" modified="2022-01-19" version="1">MKCINCSGTRTEVSRSTSSNPPNAQAFSLSQIGLQHGAAAKTEIERGLRFYRVFFQKTAQFSWAQVCDTAAKFEPLLSSHWPDYCTEMKGVAKGADVPYLSILALNVRTEIAFGLARDGCTAVFWKTETSCFLAQNWDWQEEQQMNLINLNISQEGKPAISMITEAGIIGKIGLNSRGVGVCLNAIRAQGVDFQKLPCHLALRTCLESTSTEEAVVALKKAGVASSSHLLVADSAEAVGLECSHLAIVELAMEQGIITHTNHYIKPHQGVEDLMALKDSPTRSERINQLVCEQKDRLQETGTLKLEDIRSMLKDEENFPTAICRSKTNDSSITTLFSIAMDLKNRRAEVVTGKPSSEAAMLYLVPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL57</accession>
    <name>A0A8H6CL57_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009557</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SSK1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154266.1">
        <property type="nucleotide sequence ID" value="XM_037300418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL57"/>
    <dbReference type="GeneID" id="59337952"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000146">
        <property type="entry name" value="Putative two-component response regulator SSK1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="475"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="322"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="417"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="650"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="381"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="650"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="695"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="715" mass="77897" checksum="AA14702A40611944" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLH9</accession>
    <name>A0A8H6CLH9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Transaldolase</fullName>
            <ecNumber evidence="4 10">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_009689</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the rate-limiting step of the non-oxidative phase in the pentose phosphate pathway. Catalyzes the reversible conversion of sedheptulose-7-phosphate and D-glyceraldehyde 3-phosphate into erythrose-4-phosphate and beta-D-fructose 6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transaldolase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="4 10"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154398.1">
        <property type="nucleotide sequence ID" value="XM_037300550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLH9"/>
    <dbReference type="GeneID" id="59338084"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00957">
        <property type="entry name" value="Transaldolase_TalAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000088">
        <property type="entry name" value="Transaldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004730">
        <property type="entry name" value="Transaldolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00874">
        <property type="entry name" value="talAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF18">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0704">Schiff base</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="12">
        <location>
            <begin position="246"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6225689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="626" mass="68679" checksum="F5183EE12F549366" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLP2</accession>
    <name>A0A8H6CLP2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_009700</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154409.1">
        <property type="nucleotide sequence ID" value="XM_037300561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLP2"/>
    <dbReference type="GeneID" id="59338095"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000116">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="95"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="294"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="556"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="149"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="649"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6225700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="693" mass="76866" checksum="9489B0F95013E436" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLQ6</accession>
    <name>A0A8H6CLQ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-ketoglutarate-dependent dioxygenase AlkB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009687</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154396.1">
        <property type="nucleotide sequence ID" value="XM_037300548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLQ6"/>
    <dbReference type="GeneID" id="59338082"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035516">
        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051747">
        <property type="term" value="F:cytosine C-5 DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032852">
        <property type="entry name" value="ALKBH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31573">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31573:SF4">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-ketoglutarate-dependent dioxygenase AlkB-like" evidence="2">
        <location>
            <begin position="630"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="915" mass="102072" checksum="6938C261385DBDB0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLW6</accession>
    <name>A0A8H6CLW6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009822</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154529.1">
        <property type="nucleotide sequence ID" value="XM_037300681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLW6"/>
    <dbReference type="GeneID" id="59338217"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001083">
        <property type="entry name" value="Cu_fist_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053187">
        <property type="entry name" value="Notoamide_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47256:SF1">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47256">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50073">
        <property type="entry name" value="COPPER_FIST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Copper-fist" evidence="6">
        <location>
            <begin position="150"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="736"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="23"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="768" mass="85864" checksum="CD4FB2B059353389" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMD0</accession>
    <name>A0A8H6CMD0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154626.1">
        <property type="nucleotide sequence ID" value="XM_037299800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMD0"/>
    <dbReference type="GeneID" id="59337332"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="103" mass="11860" checksum="D2995DEC7E35F999" modified="2022-01-19" version="1">MIGPEWLPRLAAPSKNLKIPPKPFDYRQNVPPLENSVSMQPDLQAQIDQALFDYDNMALPAPKIGDQFQKINDALMELTRLLFPIDPEMACELVKEQYRCEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMD8</accession>
    <name>A0A8H6CMD8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009053</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154740.1">
        <property type="nucleotide sequence ID" value="XM_037299914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMD8"/>
    <dbReference type="GeneID" id="59337448"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07105">
        <property type="entry name" value="ALDH_SaliADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000010">
        <property type="entry name" value="Gamma-aminobutyraldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000012">
        <property type="entry name" value="NAD-dependent succinate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF6">
        <property type="entry name" value="CYTOPLASMIC ALDEHYDE DEHYDROGENASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="33"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="491" mass="52675" checksum="0E44B543F16A49CC" modified="2022-01-19" version="1">MTTNGHSHGTHASNDSSNHVVPLLINGKEVTNETTFDVVSPVSGKLVWRGSSVSKRQAIEAVEAAQAAFPAWSKTKPSFRRDILLKASDILKSRGEEVCEYMDIEIGSTKAMSTYFNVPASIEQLRDVAGRIVTVTGNIPVCAEEGKSALMYKEPYGVVFGIAPWNSPFILGFRAISYALAAGNTCVLKGSEISPRCFWAIGSILKAAGLPDGVLNVIFHRPQDAPEVTSAIIEHPFVKKINFTGSTATGSIIAAQAGKFLKPVLMELGGKAPAIVLEDANLQKAAEGCAVGAFLQSGQICMSTERILVHSSIAEKFREALKAAMEKMYGKESSAPILVNAPPVQKNKKLVEQALSKGAKILTGDLNAKEHSDTRMRPIVVDGVTKDMDLYQIESFGPTVSLIVVESEEEAIEIANDTEYGLSSAVFTEDLAAGLRVAKQIETGAVHINNMTVHDESNLPHGGAKKSGWGRFNGSAGIEEFLRIKCVTWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMK7</accession>
    <name>A0A8H6CMK7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154746.1">
        <property type="nucleotide sequence ID" value="XM_037299920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMK7"/>
    <dbReference type="GeneID" id="59337454"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="240" mass="27371" checksum="1CD8727BCC248F44" modified="2022-01-19" version="1">MSWAQPRGGKEGRRQRGGEGLDSYCDCAMDRKIYRPSGGELERRIDVPHQSEMPPRTRPGYCLHGQQELPLPPPQDSSLDWQEERRLPPRPNPSPGWQEKCRLPPPRRWSPGWYEERPLADSDTLPRHWRDAQLAKRDTRGTNQGQPTSKGARVHFQRPAECRQRTPEDEEEEEESGSSPGNSLRGSVAHIEEGEKSDDRDPGISDYFDMIEHEGAGSGDEDDYSLNAMELSLRRGFGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMN4</accession>
    <name>A0A8H6CMN4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF1772-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the anthrone oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154748.1">
        <property type="nucleotide sequence ID" value="XM_037299922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMN4"/>
    <dbReference type="GeneID" id="59337456"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013901">
        <property type="entry name" value="Anthrone_oxy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35042">
        <property type="entry name" value="ANTHRONE OXYGENASE ENCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35042:SF1">
        <property type="entry name" value="DUF1772-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08592">
        <property type="entry name" value="Anthrone_oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="18829" checksum="1C028B9522CE48C6" modified="2022-01-19" version="1">MAPLPAAIRVAQTIGITASAFCSGSILSISILTIPTWLIAPSPVLVRQWQLCFDRGKILNPAIALVSIISYSYLSYRLYGTLNHPKAEMYGLSALATFGIWPYTIFGMMPTNRKLFKKHDEMKGLDVGEKATEVGLAKGESAKELVDRWGMLNLGRSVFPLVGAVLGLWATLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMS9</accession>
    <name>A0A8H6CMS9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009248</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154935.1">
        <property type="nucleotide sequence ID" value="XM_037300109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMS9"/>
    <dbReference type="GeneID" id="59337643"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09535">
        <property type="entry name" value="SAM_BOI-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11886">
        <property type="entry name" value="SH3_BOI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000082">
        <property type="entry name" value="Polarized growth protein boi2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035551">
        <property type="entry name" value="Boi1/2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037370">
        <property type="entry name" value="Pleckstrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092:SF16">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092">
        <property type="entry name" value="PLECKSTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="14"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="6">
        <location>
            <begin position="252"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="776"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="121"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="321"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="438"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="523"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="630"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="691"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="971"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="327"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="351"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="440"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="523"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="544"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="633"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="706"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="725"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="735"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="764"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1008"/>
            <end position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1018" mass="110809" checksum="64D5884350F32FCE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMX1</accession>
    <name>A0A8H6CMX1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal RNA-processing protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009259</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154946.1">
        <property type="nucleotide sequence ID" value="XM_037300120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMX1"/>
    <dbReference type="GeneID" id="59337654"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12293">
        <property type="entry name" value="dRRM_Rrp7p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12950">
        <property type="entry name" value="RRP7_Rrp7p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040447">
        <property type="entry name" value="RRM_Rrp7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040446">
        <property type="entry name" value="RRP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024326">
        <property type="entry name" value="RRP7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 7 HOMOLOG A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17799">
        <property type="entry name" value="RRM_Rrp7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12923">
        <property type="entry name" value="RRP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rrp7 RRM-like N-terminal" evidence="4">
        <location>
            <begin position="10"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 7 C-terminal" evidence="3">
        <location>
            <begin position="198"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="258"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="35279" checksum="1156222ECC08B2E4" modified="2022-01-19" version="1">MSSLTKIPLQISGYNILPLSLPPLPSFPEAATHYLYLVPHQPKIPTPTASRSLFLVNVPFDSTELHIKLLFSTQIGLPAGRIEDVQFEGQRRRGSGTDQASSPKASQNKKSKKRKRGSSGGIIEDMEGAALPPVWDRDLQTNGLTAVVLFVDRASMDAALKAVKSIRKERKEPLWGEGLEGKVPALGSARYLNHHSLTYPNKAQLLESVNNYMTRFASKEASQARSLARQRQVADADGFITVTRGGRTNPARQEVAQEQAEKQKEKQKGLDDFYRFQSREKRKQRAGELVRKFEEDKEKVRKMRERRGRFRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNE1</accession>
    <name>A0A8H6CNE1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_008316</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cytochrome c oxidase subunit which plays a role in assembly of respiratory supercomplexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Associates with the respiratory chain complex III/complex IV supercomplex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RCF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155184.1">
        <property type="nucleotide sequence ID" value="XM_037299183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNE1"/>
    <dbReference type="GeneID" id="59336713"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050355">
        <property type="entry name" value="RCF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297:SF3">
        <property type="entry name" value="HIG1 DOMAIN FAMILY MEMBER 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297">
        <property type="entry name" value="HYPOXIA-INDUCBILE GENE 1 HIG1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="HIG1" evidence="10">
        <location>
            <begin position="4"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="188"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6226875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="251" mass="29571" checksum="CE4D702AD7C4A2E0" modified="2022-01-19" version="1">MSDAPLPSSFDADFFEENRWQKFTRRLKEEPLIPFGVAVTCWALYNASRSIRQGNGARTNQFFRYRLYAQSFTLVAMLGGSYYYNADRLKRKEYTDLAKKRKAQVKRDAWIRELEARDQEDREWREKLGRVRDFQREEVEREAIEEMKMREGRSDDGRGVIDALKGKMKDVKNTEANKEAAELEQRKQQIKEKRREMEKKQEVERMKQAVAKQEAGMGPDTRVWGEAGGGLFGWKHIRNWFNGPRDGEGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNJ9</accession>
    <name>A0A8H6CNJ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009247</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154934.1">
        <property type="nucleotide sequence ID" value="XM_037300108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNJ9"/>
    <dbReference type="GeneID" id="59337642"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12611">
        <property type="entry name" value="RRM1_NGR1_NAM8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12613">
        <property type="entry name" value="RRM2_NGR1_NAM8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12346">
        <property type="entry name" value="RRM3_NGR1_NAM8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000065">
        <property type="entry name" value="mRNA binding post-transcriptional regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000222">
        <property type="entry name" value="mRNA binding post-transcriptional regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000227">
        <property type="entry name" value="mRNA binding post-transcriptional regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF10">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="66"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="159"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="292"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="230"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="361"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="811"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="846"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="951"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="992"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="951"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1004"/>
            <end position="1030"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1173" mass="126002" checksum="EBB866A4652E788F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNN1</accession>
    <name>A0A8H6CNN1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008288</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155156.1">
        <property type="nucleotide sequence ID" value="XM_037299155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNN1"/>
    <dbReference type="GeneID" id="59336685"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019622">
        <property type="entry name" value="Rrn9_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF52">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10680">
        <property type="entry name" value="RRN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rrn9" evidence="7">
        <location>
            <begin position="119"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="236"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="317"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1015" mass="114632" checksum="8DF7868162A0ECA0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNV1</accession>
    <name>A0A8H6CNV1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155177.1">
        <property type="nucleotide sequence ID" value="XM_037299176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNV1"/>
    <dbReference type="GeneID" id="59336706"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="679"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="704"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="754"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="790"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="816"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="870"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="881"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="900"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1023" mass="110167" checksum="67FFFED6F7A45E6E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPP7</accession>
    <name>A0A8H6CPP7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_008780</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155645.1">
        <property type="nucleotide sequence ID" value="XM_037299644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPP7"/>
    <dbReference type="GeneID" id="59337175"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017126">
        <property type="entry name" value="Condensin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="188"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="289"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="603"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="202"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="300"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="603"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR017126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6227337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="839" mass="91294" checksum="1510280BE89C3B24" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQE9</accession>
    <name>A0A8H6CQE9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_007683</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155889.1">
        <property type="nucleotide sequence ID" value="XM_037298553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQE9"/>
    <dbReference type="GeneID" id="59336080"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008726">
        <property type="term" value="F:alkanesulfonate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046306">
        <property type="term" value="P:alkanesulfonate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01094">
        <property type="entry name" value="Alkanesulfonate_monoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024014">
        <property type="entry name" value="DMSO2_SphG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050172">
        <property type="entry name" value="SsuD_RutA_monooxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04021">
        <property type="entry name" value="LLM_DMSO2_sfnG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42847">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42847:SF4">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="9">
        <location>
            <begin position="71"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="7">
        <location>
            <begin position="246"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Luciferase-like" evidence="8">
        <location>
            <begin position="474"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6227955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="835" mass="92258" checksum="CC5D177FE8EE9805" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR68</accession>
    <name>A0A8H6CR68_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156094.1">
        <property type="nucleotide sequence ID" value="XM_037298758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR68"/>
    <dbReference type="GeneID" id="59336287"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="243" mass="27877" checksum="08738179560F5B67" modified="2022-01-19" version="1">MYEEVLWITSSPSLPGLPTELVVRSLELIPNCCAVAALPESPSAQTLPKSGYLTRPQHRTLFSLEPSNVTLTLKNWFDFKQHRNRECEAAEESETDEPQTGEADDAHHNVLKRNEYFAVDASIVSKECADLQAEAISDFDQDLHRSEAKGLRRTGFRGLKRIRYRIHCIAALSSHRAAQDGYLEATSLDDLKGMRDDSAGTKRRHYYIDFDYFRIHIKTSWARNAFDSIEEVYRDKCCRKYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS22</accession>
    <name>A0A8H6CS22_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_007926</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156130.1">
        <property type="nucleotide sequence ID" value="XM_037298794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS22"/>
    <dbReference type="GeneID" id="59336323"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045315">
        <property type="entry name" value="Mtm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760:SF2">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="59"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="228"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="343"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6228196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="49667" checksum="2728B20E11C9CACE" modified="2022-01-19" version="1">MSGFISEPARTDEHQIATPSHGNSEQPPGQRHNISSKGAASSNSVTMNDSGEPEVEMDISAGQKMLSAVSGSLLTSLLVTPLDVVRVRLQSQSQTSNLPISKPSSNFSNLPPNIGVTACCREVFWVNNTSPFCIAANPSPTATLATLNPSLSSTASPAADCAVDETRRKNFTSTLDGLRKIARHEGSTSLWRGLSPTLVMSIPANVIYFTGYDSLRSNPHSPVHNYIPDAYAPLIAGSIARVAAASVISPIEMFRTRMQAATSGSGTGVFAETLTGLRTMVHSRGYTSLWRGLTLTMWRDVPFSGIYWWGYEAVRNRLTDMRDPPHSRTRSRSKPRDSGNHTSTFVDSFIAGALSGAIASVITTPFDVGKTRQQVLHHEPAKEIGREASTALRPEEQAMPRFIYHIWKEEGMAGLFRGWVPRMLKVAPACAIMISSYEVGKKMAKRMNEKKRDAAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6J0</accession>
    <name>A0A8H6F6J0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gamma-glutamyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006845</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146942.1">
        <property type="nucleotide sequence ID" value="XM_037297741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6J0"/>
    <dbReference type="GeneID" id="59335245"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000001">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF62">
        <property type="entry name" value="GLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="330"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="382"/>
            <end position="383"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6217507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="54128" checksum="DED946407DC58141" modified="2022-01-19" version="1">MTDSALSPLRTDGLGGGGFALVRSANGSYESVDFRETAPAAAFRDMYKNNVLGSVFGGLASGVPGEIKGLHYIHENHGFLPWSTLVQPAINLARDGFIVGRDFAKAMALTTAYYGYDFLSKEPTWAEDFAPNGTRVEAGDVMTRKRYARTLEAIAVSGPDAFYEGQLAEATIRALHETNGSMTMEDLAKYRIISRTPVQIEYKGFRLHGCGAPASGAVALSVMKVLEGYEDFGQRHTMNLSTHRLDEAIRFGYGKRASLGDPDFVDGITAYEAGMLDQTSADEIRARISDRHTLNVSDYDPDGFESHENHGTSHVATADASGMAISMTTTVNLFFGSGVMVPETGVIMNDQMADFSVPNISNVFGYYPSPANYVRPNKRSMSSITPIMAESANGTLFAILGASGGSRIITTVIQTALNILERKMTVHQALKQPRLHDQLLPNQVTFEWDYDNSTIDYMIGRSHNVTWGPAGSSSQAIGITNGHFEAAGEPRQHDSAGLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7J8</accession>
    <name>A0A8H6F7J8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147632.1">
        <property type="nucleotide sequence ID" value="XM_037297058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7J8"/>
    <dbReference type="GeneID" id="59334560"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="211" mass="23214" checksum="4A2064D30F76AB22" modified="2022-01-19" version="1">MSSQIFREPFNRIRSPRAHPRTSMPDNDTQALSSSASIRRPNFPNTDRVLRLVLLKSVTQRPPGPLRPREGKGPQTTGDVPQRQEVVIRSNKAKINGLLLADGESSSTALTGIMNSLLRSPSELAILVDDIRTALARETDMISTAVHELRHLGVFIEEKLRLAPSVPVDLARVVGHFGTGHWILVVWPSVIICARSNPTPHERAAPGNTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7M4</accession>
    <name>A0A8H6F7M4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147148.1">
        <property type="nucleotide sequence ID" value="XM_037297438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7M4"/>
    <dbReference type="GeneID" id="59334941"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="281"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="37751" checksum="87854AE3175B5A76" modified="2022-01-19" version="1">MDQAVSFDNGAITLEHLKDCDLKHLPFETPAEIERYWTLAAECIKSDFVIEGGTPRVDIDMEDNPTCKLPAEIIQMEQASCAGDVAAAEDVYHEWLAKPQDSERAHINHFASSFAIAMAHGHVSIASYLLRVGVPMNYRHFELAIEHKSYAFLQLYLDYGYNINESPDWATPPPLFQAFQDDALTRWLLDHDADPNAQCVLDLTPLSIAMCYAPFPTIEMLFRYGGSIEHGQLLHHAVRRVLADRVKVVNYILDMGPPINDIMYQNQPYNYSLQEDFGIGTALHGAAEHGYLDVVELLLARGADPLAKDAKGKLPIERARREGRKEIVNFLLPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7M8</accession>
    <name>A0A8H6F7M8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argonaute linker 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147104.1">
        <property type="nucleotide sequence ID" value="XM_037297668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7M8"/>
    <dbReference type="GeneID" id="59335172"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Argonaute linker 1" evidence="1">
        <location>
            <begin position="119"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="257" mass="28798" checksum="377B68A345BA3EA0" modified="2022-01-19" version="1">MGDSLARIQTIDVDEGLFPIRPDLGNTSIQESVQLWANYFVMELHKRDLVLYLYDMAFEAFPPNGSDQTDKEISVPEGNKLVQVIRCALNLSTFNEIKFDIATDSRTNNLNRFPLQSPRDTPSYLQGVRGFFASVRATTGSTLVNCNACCGAFYKSGPLEHLFAVFIPSNRSPSPEHFKWLEKAIQGLRVEFNHLKDDKGNQVGSLLTIFGLARPYGGLKDVELYHKEDEQAYTVATYWAKKGTSLPTSAFEVSDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7W6</accession>
    <name>A0A8H6F7W6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WKF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147977.1">
        <property type="nucleotide sequence ID" value="XM_037296796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7W6"/>
    <dbReference type="GeneID" id="59334296"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019327">
        <property type="entry name" value="WKF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10180">
        <property type="entry name" value="WKF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WKF" evidence="3">
        <location>
            <begin position="278"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="508"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="436"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="549"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="600"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="610"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="628" mass="67891" checksum="62D37103F27050B2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7Y7</accession>
    <name>A0A8H6F7Y7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Chitin synthase</fullName>
            <ecNumber evidence="2 11">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_005889</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the chitin synthase family. Class III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2 11"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147975.1">
        <property type="nucleotide sequence ID" value="XM_037296794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7Y7"/>
    <dbReference type="GeneID" id="59334294"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF11">
        <property type="entry name" value="CHITIN SYNTHASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="544"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="573"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="632"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="661"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="714"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="841"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="881"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="13">
        <location>
            <begin position="146"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="103"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6218540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="916" mass="102210" checksum="74AAD1EE3427C6AC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7Z7</accession>
    <name>A0A8H6F7Z7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005920</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148005.1">
        <property type="nucleotide sequence ID" value="XM_037296824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7Z7"/>
    <dbReference type="GeneID" id="59334325"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034439765" description="Peptidase A1 domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="5">
        <location>
            <begin position="55"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="500"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="547"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="651"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="730" mass="77868" checksum="B249D53C6B2F9B08" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8B2</accession>
    <name>A0A8H6F8B2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005521</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148412.1">
        <property type="nucleotide sequence ID" value="XM_037296430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8B2"/>
    <dbReference type="GeneID" id="59333927"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025755">
        <property type="entry name" value="Ribos_uL4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14374">
        <property type="entry name" value="Ribos_L4_asso_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL4 C-terminal" evidence="5">
        <location>
            <begin position="280"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="372" mass="39927" checksum="26EBB30CD8EE479A" modified="2022-01-19" version="1">MATRPTVTIASHDGTPSGSTHPLPAVFKAPIRPDIVQTVHAGMAKNKRQPYAVSEKAGHQTSAESWGTGRAVARIPRVSGGGTHRAGQAAFGNMCRSGRMFAPTKVWRKWHQKINLGQKRFATTSALAASSVPSLLLARGHQISTVPEVPLVIASKAFEGAAIAKTSAALKLLTAVGAGPDVSKVRASRKLRAGKGKLRGRRHRQRRGPLIVYHPDEDGKELVRAFRNIPGVETSSVFALNLLQLAPGGHLGRFIVWTSSAFAALDEIYGSTTTPSALKKDFLLPSNVVHNADISRLINSSEVQAVVRPAKGEAKTKRAGVQKKNPLKNKQVLLRLNPYAKAFSEEKLGSKKLEDGKPERAAESFAKVLHEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8C4</accession>
    <name>A0A8H6F8C4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polysaccharide export protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148171.1">
        <property type="nucleotide sequence ID" value="XM_037296189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8C4"/>
    <dbReference type="GeneID" id="59333684"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021047">
        <property type="entry name" value="Mannosyltransferase_CMT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34144">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34144:SF7">
        <property type="entry name" value="EXPORT PROTEIN (CAP59), PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11735">
        <property type="entry name" value="CAP59_mtransfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="45887" checksum="611889105E8726F9" modified="2022-01-19" version="1">MKRTSLRRFSRSSRTRVFRATLLVIALFCIIDVLSLISARQHQNLPAKTPDVRGQKIFIASIHWNNDPILRSNWNQAVLGLVDYFGAENVYVSVYESGSWDDSKETLRVLDNDLERKGVRRTVRLNETTHADEMRQPETDGWIQTPRGKTELRRIPYLSKLRNLALEPLNYLEDGTKYDRVLFLNDVVFTTEDVATLIATRDGDYSAACSLDFAKPPHYYDTFALRDDDGHEAITSTFPYFRSKASRSAMISGQPVPVQSCWNGIVAFDAAPFYDPNRLKFRGVSDSLALHHVEGSECCLIHADNPLTQSHGVWLNPNVRVGYSPEAYAAVKSALAWPSMYQSLLGIWSNRLWRWTTSTKMKSVRITSRLKSWSEDGADRSEPGMFCLINEMQVLIENGWAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9C0</accession>
    <name>A0A8H6F9C0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149415.1">
        <property type="nucleotide sequence ID" value="XM_037294727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9C0"/>
    <dbReference type="GeneID" id="59332216"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="273" mass="31410" checksum="E742CA7B0B54FEE9" modified="2022-01-19" version="1">MAGTSGCIPDYHVNLFSKLCKPAPNTSIMTASEPGPQADTNDHINEAPVPSSTSVRAPEPDPILQLFNENQNLIRGLSLGNAETYLAAVKEVFAEVRRDRANACVELPRKQRGADHRKVELIDEKQAEHQDEHGTEFQDQHNDQQRDRQQAEQGVDPEDQHQNQYRVQHPKQHQPHQEEHQPQHQPQHQPQSPPKHPAHPFIITHYSKENDEDKKPRQSRLQREADAMYYIEDDTEDRETDCDEFRGLDALLMPNFTGDPRKKYGFDQMRLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA52</accession>
    <name>A0A8H6FA52_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002638</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150392.1">
        <property type="nucleotide sequence ID" value="XM_037293563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA52"/>
    <dbReference type="GeneID" id="59331050"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000002">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14716" checksum="8E259E56CDF25342" modified="2022-01-19" version="1">MSSSKVRTGQLWGKSKDDLKKQLVDLKTELGQLRTQKIAGGASSKLHKIHDLRKSVARVLTVINSNQRQQLRIFYAKKKYLPLDLRPKKTRAIRRRLSKHEATIVTEKQKKKSTHFPQRKYAVKAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAL8</accession>
    <name>A0A8H6FAL8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calcipressin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RCAN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150810.1">
        <property type="nucleotide sequence ID" value="XM_037293157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAL8"/>
    <dbReference type="GeneID" id="59330644"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008597">
        <property type="term" value="F:calcium-dependent protein serine/threonine phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000503">
        <property type="entry name" value="Calcineurin binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006931">
        <property type="entry name" value="Calcipressin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300">
        <property type="entry name" value="CALCIPRESSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300:SF14">
        <property type="entry name" value="PROTEIN SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04847">
        <property type="entry name" value="Calcipressin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="30581" checksum="662FED5F1B04C2B7" modified="2022-01-19" version="1">MPAQDKSPPASPSESPTSTSISNGTQTPLSLDLSALPPLITPSPPSNTLLITNLNSLATFLPQNLQHIHTLLSTPTPLNSFSPLRSFRRIIVSYPTVEAAIEIRQLLDGSPLSENDRVRIYFGEPTPILGDGGEDQHLKAPSLGKLLFISPPPSPPAGWEIREEEPPNKDVHAEDLQRALAGLGNHGKERRASADDAGSPMSEDEKEELQGNRKRSGTGSMMVYHPKHHGDNESLPAVMVIDTTAGAEEEGQESPGSSSPVDGRGQGGGRILTHTARPPVELMEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAN2</accession>
    <name>A0A8H6FAN2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanine(10)-N(2))-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.214</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_002573</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.214" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150328.1">
        <property type="nucleotide sequence ID" value="XM_037293499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAN2"/>
    <dbReference type="GeneID" id="59330986"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016691">
        <property type="entry name" value="TRMT11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059073">
        <property type="entry name" value="TRMT11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25904">
        <property type="entry name" value="Tmrp11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51627">
        <property type="entry name" value="SAM_MT_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 10" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA (guanine(10)-N(2))-methyltransferase TRMT11 N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="13">
        <location>
            <begin position="177"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="432"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="461"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6220893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="520" mass="58837" checksum="6A89757CF3B76C09" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAU9</accession>
    <name>A0A8H6FAU9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001566</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151035.1">
        <property type="nucleotide sequence ID" value="XM_037292496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAU9"/>
    <dbReference type="GeneID" id="59329982"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054464">
        <property type="entry name" value="ULD_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886:SF1">
        <property type="entry name" value="NACHT-NTPASE AND P-LOOP NTPASES N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886">
        <property type="entry name" value="SESA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22893">
        <property type="entry name" value="ULD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="251"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="570" mass="64629" checksum="E4EF32E73C22BFF2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBD4</accession>
    <name>A0A8H6FBD4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="2">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001869</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151336.1">
        <property type="nucleotide sequence ID" value="XM_037292797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBD4"/>
    <dbReference type="GeneID" id="59330283"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06822">
        <property type="entry name" value="PLPDE_III_YBL036c_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000007">
        <property type="entry name" value="Pyridoxal phosphate homeostasis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00044">
        <property type="entry name" value="YggS family pyridoxal phosphate-dependent enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PROLINE SYNTHETASE CO-TRANSCRIBED BACTERIAL HOMOLOG PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146:SF14">
        <property type="entry name" value="PYRIDOXAL PHOSPHATE HOMEOSTASIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004848">
        <property type="entry name" value="YBL036c_PLPDEIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01211">
        <property type="entry name" value="UPF0001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine racemase N-terminal" evidence="6">
        <location>
            <begin position="27"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2 3">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="278" mass="30653" checksum="AB26F94541CEFB5C" modified="2022-01-19" version="1">MTDTKDQEPAEMRVDPARAKHLSSNISSILSRIESAKPSSGPPVRLVAVSKLKPANDVLALHSAPHNHLHFGENYQQELLQKSLLLPREIRWHFIGALQTNKCKPLAEQIPNLWCVESVDTGKKADQLEKGRAALCEKEKDYGGVPLRVFVQANTSGEEEKSGVEPEGAAELCRHVREQCPHLKLQGLMTIGAIARSKEAKEGVENEDFSRLREVRDQVVKELGLQEGDLELSMGMSEDFESAIRCGSNEVRVGSTIFGERPAKGDAKIKETVEEGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBJ2</accession>
    <name>A0A8H6FBJ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001717</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151184.1">
        <property type="nucleotide sequence ID" value="XM_037292645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBJ2"/>
    <dbReference type="GeneID" id="59330131"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24073">
        <property type="entry name" value="DRAB5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="274" mass="30293" checksum="996E0DE2D70879C1" modified="2022-01-19" version="1">MSNSLEAKICVLGAQGVGKSSLLDRYIKGSFSPSSVTSTIGASFLTKRVVDVDTGTTVRLQLWDTAGQERFRSITKLYYRGASAVLLVYSIIDESSFEEMGRWLTEMRENLGNDIVIHVVGTKSDVVAQEPSLRQIPFERCIAYVAENLYPQKVSTPTFPGSARSSGFWGQEIGWDCCHEISASNGEGIEEVFRVITRKLVEQKNKKEQEQLRDERNNTPGQKRGQGGYFEGLSDVQGSFKLSNKRQSWLGIATGEEANDGAQSLAINRKGKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBR6</accession>
    <name>A0A8H6FBR6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">aldehyde dehydrogenase (NAD(+))</fullName>
            <ecNumber evidence="5">1.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001972</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6222004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151439.1">
        <property type="nucleotide sequence ID" value="XM_037292900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBR6"/>
    <dbReference type="GeneID" id="59330386"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07090">
        <property type="entry name" value="ALDH_F9_TMBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000014">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009725">
        <property type="entry name" value="PRK13252.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="25"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6222004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="498" mass="53654" checksum="CB68B32B727C5372" modified="2022-01-19" version="1">MADSFYPPRHLYYDGQVQVGSGGIFRSIDPSTAQPLADIHAASHSDIDSAIQSGQRAFHDWSTTPPIVRSRILLKAVQLLRDRNDEIARTETQDTGKPFSETSTVDVVTGADVLEFFANLVGGGGLNGETTQLREDAWVYTKKEALGVCAGIGAWNYPIQIALWKSAPCLAAGNTMVYKPSEVTPLHGQILAEIYTEAGLPPGVFNVVQGGGDVGAYLTSHVGIAKVSFTGQVSTGKKVASSAAAGMKYTTMELGGKSPVIILSDVDLEQAVNGAMMANFFSTGQVCTNGTRVFIPKDMKQAFEEMLLEKMKYIRAGNLMDVNTNFGPLVSEMHYEKVIGYIKYGLEKDRATLLCGGPDQPKDIPGLEGGYWVRPTVFTDCTDSMRIVQEEIFGPVMTLLTYESVEEAVERANATPLGLAAGVFTKDLNMAHKVVAKLEAGITWVNTWGESPAEMSVGGWKQSGVGVENGKRGLEAWVRNKSTLVDMSGTVPTVFSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDD8</accession>
    <name>A0A8H6FDD8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">General stress protein FMN-binding split barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010954</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153437.1">
        <property type="nucleotide sequence ID" value="XM_037301803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDD8"/>
    <dbReference type="GeneID" id="59339344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052917">
        <property type="entry name" value="Stress-Dev_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038725">
        <property type="entry name" value="YdaG_split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34818">
        <property type="entry name" value="PROTEIN BLI-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34818:SF1">
        <property type="entry name" value="PROTEIN BLI-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16242">
        <property type="entry name" value="Pyrid_ox_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="General stress protein FMN-binding split barrel" evidence="2">
        <location>
            <begin position="29"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="202" mass="21741" checksum="B47A6EC2127E655A" modified="2022-01-19" version="1">MSFSNAKVPDDKPADPYKATNLTDPDLKEKVQDLVSFMESCKFGMMTTRIESSGLLTSRCMALAAKEGGGVDLLFHTNTESGKTDDLKSEPKINIAFLNPTGEWASVSGEADIVTDRETIRKYYSPALKAWLGDLEDGKHDGGPEDPRIGVIKVKALTATYAIATGTSLGRGADIVKGAITGAAPKVNKLREISEEELQQCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE79</accession>
    <name>A0A8H6FE79_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleolar GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010020</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Nucleus</location>
            <location evidence="2 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. NOG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153693.1">
        <property type="nucleotide sequence ID" value="XM_037300876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE79"/>
    <dbReference type="GeneID" id="59338412"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000496">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024926">
        <property type="entry name" value="NOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038919">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="9">
        <location>
            <begin position="168"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="546"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="652" mass="74308" checksum="B11F6210B10B8908" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FED1</accession>
    <name>A0A8H6FED1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154142.1">
        <property type="nucleotide sequence ID" value="XM_037301325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FED1"/>
    <dbReference type="GeneID" id="59338863"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="391" mass="45151" checksum="D96A6F05212A37E1" modified="2022-01-19" version="1">MSFISAPSPEPDLHKLLLSEDPDDALDKRLEDHITMSLWSTQQHHNSAAYFLYFREECEAWRLSGSAVAIQTYQDFLDLVEHLKRTRNEKRSAAAVLDFFPPVRAADDEKTPTPSLTEECTSGQLHWSLRHRSPRCDLVSATNSIFLTVRLWLMLNVGSSANLTLFPGRSSPEWMEDQSLDNFIDSCFPVSEWGPRLSQWPFSLNAYNLERVGGFELVWTDHLADHLYLNEDLGTISIYHHVHVLRGLLQAKSPNQALPDRLLTETLQTLALIIPRANRDCKTWFEKVHNKYAKYAENIDHGAGDVELLHWARSTEKYKFWGQRLITIKMAYDASEPKNLGQWWRDRRRRVEWYTFWVAVLVVFLTIVFGLIQSITGVMQVYYAAHSGQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FED4</accession>
    <name>A0A8H6FED4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010171</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM170 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153843.1">
        <property type="nucleotide sequence ID" value="XM_037301026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FED4"/>
    <dbReference type="GeneID" id="59338563"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019334">
        <property type="entry name" value="TMEM170A/B/YPR153W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779:SF6">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10190">
        <property type="entry name" value="Tmemb_170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="211" mass="23745" checksum="504FE7648A15B2EE" modified="2022-01-19" version="1">MPASANTTTWVPENYKTPSFPSLYWLVGPDNLVQPRFLYHVHDIWRFTLFWTTIIFEAVHLLVGTYAVVIVWWGGRNKLKEDGKENGGGKEVKRGREESLKNIKVLWVVPVVYGIVAGVEALLAGSVVGLILGAVYNAGGFRMSTWIPRTMTKADDLPECERTPKLRTSTWHAQATTAFLSRNISQQTHEQEHCKAEVNRNGLALGIQLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FET1</accession>
    <name>A0A8H6FET1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154066.1">
        <property type="nucleotide sequence ID" value="XM_037301249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FET1"/>
    <dbReference type="GeneID" id="59338787"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411:SF3">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="191" mass="21892" checksum="5A08E7D2681DF7E9" modified="2022-01-19" version="1">MDQEFSTDVILGKGGDLVILLHGILRLGKTSTAERAAVQIRRPVFHITCGDIDTTRGQVEERSNGYFDVAYKRQMGNAFETAISLAKWDYQENGSKSDEQLCLSLEQLKVLLRRAHISTPTYPPCKNETPREHSGGSAHLTRRRRNRRSRRKAERLYEDEDKDSDSDGNEAKMKKLKAELERRRKIKKSIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FET2</accession>
    <name>A0A8H6FET2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Mediator complex subunit 20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED20</name>
        <name evidence="5" type="ORF">HO133_010425</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Mediator complex subunit 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FET2"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013921">
        <property type="entry name" value="Mediator_Med20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08612">
        <property type="entry name" value="Med20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="27140" checksum="2750030661C3698A" modified="2022-01-19" version="1">MPVTALYFLPASGPDSNPTQDILAKLTSYYDLSPLPPWTLSHRIFRETPFTQPSAQPFPSLTNGVDKAKPPGPRFLQILSLSHHAPRTFVAITTTHSASQIKAATPASSNISAEAASGEPATIISIAAGPSSEEFIQLIISKFGPLWQQRQILSAGYGHAFEIGDFRVRVGELRQGGSGGGQMGRGAVCEVTWIREEAEQEQEGWEVGEAVVKGFWEGLALRGAREVYWVPGLGDGEGTIRQWCELLRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEY6</accession>
    <name>A0A8H6FEY6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154345.1">
        <property type="nucleotide sequence ID" value="XM_037300497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEY6"/>
    <dbReference type="GeneID" id="59338031"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="312" mass="34952" checksum="5C84E5E24E0FCD30" modified="2022-01-19" version="1">MGLNRGPIGPWVVRDICLTAQAILAGIVVELEDSELARWALRLRLWGTELFGADSVEQDEVVETIPASTTHRPIPSLVEMLSDLLPALRAANRARCVGQGDGSLSYNEQYFANANGQLNDADEILKKVDEAAKEKEGPTYTLRLQQERECLERVHLDCKSGRDIDGEVLKEIPWEQIELDVNLSLFLARSNDAALRLKRPKFKNKVRREDHQDRERAEILISILRDTDRGLKDLLHGNGQIVKGSESPPRHILRYLLIPRGFRASNLRSLASSPAEDDEDSTGAGEKGDIGIAKAIVESSDLFHLAFRIIYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG47</accession>
    <name>A0A8H6FG47_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">threonine synthase</fullName>
            <ecNumber evidence="4">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008501</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the threonine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155368.1">
        <property type="nucleotide sequence ID" value="XM_037299367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG47"/>
    <dbReference type="GeneID" id="59336897"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000024">
        <property type="entry name" value="Probable threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="entry name" value="Threonine synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051166">
        <property type="entry name" value="Threonine_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690">
        <property type="entry name" value="THREONINE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690:SF1">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24857">
        <property type="entry name" value="THR4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="9">
        <location>
            <begin position="69"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6227060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="495" mass="55414" checksum="A3B61EFFCA0C3B42" modified="2022-01-19" version="1">MHVLPKRPSHTLCSYVSQRSFEEVVITGLASDELAFEIFALYIDPNEIPSRDLKEIVNKSYSTFRAPDVTPLVTLDQEKQLYLLELFHGPTFAFKDVALQLLGNLFEYFLVRKNGDKPESTREHLTVVGATSGDTGSAAIYGLRSKKDVDIMIMFPEGKVSPVQEAQMTSVLDQNVHNVAVDGSFDDCQDFVKELFGDAEIKRTHQLAAVNSINWARILAQIDVLAGYFAKRMGFPIEKLVVATNENDILDRFWKTGYYEKKPIHGRQVNGGFAEDGVKAHEDGVRETFSPAMDILVSSNFERLLWFLAFQVYGEGLKTVEEKRAEAGRRVKGWLDELKSKGGFGVEPKLLEAAKLDFESERVSNKQTIAMIRQQYSAQTPAAKEDASSGTAGATNHGKYILDPHSAIGVEAALRSVQRAPPPGTTHIALATAHPAKFSKAVSQALENEKNFQFRDLLPEQFVGLEELEKRKYHFRKSEGIEGLRNFIRKRVPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG86</accession>
    <name>A0A8H6FG86_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008851</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155715.1">
        <property type="nucleotide sequence ID" value="XM_037299714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG86"/>
    <dbReference type="GeneID" id="59337246"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10567">
        <property type="entry name" value="SWIB-MDM2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="3">
        <location>
            <begin position="5"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="208"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="32503" checksum="38C6C462206D16EA" modified="2022-01-19" version="1">MSLPTEVEASFTTIIDSILAASDLDTISEKRIRQGLQQSVEYDITPQKTAIKALIMRRFDKFDEQRKAPPQTNGHASVKEESSASPQTFSHTSVKQGSLASQQTNGHTTVKENSPLSSEPTRKATSQSPKKHKQESDEEELSDVKNTPPPKKKKKVERDSDAAYAAKLQAQENARTRSTRGGGPKAPPPKKNKKSPKKKTSAKVKAEDDSDIDASGSDVKEKVRSDNEIKLSRPQTVKRIWAYVRAKDLQDPSDKRMIRCDDALRAVFKQDKVHMFTMNKILSQNLYVPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHD6</accession>
    <name>A0A8H6FHD6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.205</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2'-O-ribose RNA methyltransferase TRM7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_007671</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>cytidine(32)/guanosine(34) in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42396"/>
            <dbReference type="Rhea" id="RHEA-COMP:10246"/>
            <dbReference type="Rhea" id="RHEA-COMP:10247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.205"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.205" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155877.1">
        <property type="nucleotide sequence ID" value="XM_037298541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHD6"/>
    <dbReference type="GeneID" id="59336068"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106340">
        <property type="term" value="F:tRNA (guanosine(34)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03162">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028590">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF12">
        <property type="entry name" value="TRNA (CYTIDINE(32)_GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="9">
        <location>
            <begin position="21"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="276"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="345"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6227943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="380" mass="41922" checksum="9670EE09A9A67E66" modified="2022-01-19" version="1">MGKSSKDKRDAYYRLAKEQGWRARSAFKLIQLDEQFDLFSGVTRVVDLCAAPGSWSQVLSRVLIKGESFGRRAWEEKQKREGEALPGGVEEKEGDGHRDGEEEKQVKLGELRPRKDVKIVAVDLQPMASLEGITSLKGDITTPSTVTRILQALDPDTYVNDAEDSGSHPVDLVLSDGAPDVTGLHDLDIYIQSQLLYSALNLALRILKPGGKFVAKVFRGKDVDLTYAQLRCFFTSVHCAKPRSSRASSLEAFVVCEGFNIPKGYKVDPKRPVGLGAQVPVPKKKQKDIQSSDGGGQRRTVREDGVSVLDLESDGEEDKNRWIAPFVACGDLSAWDADATYHLPKDRVSLDPVQPPTAPPYKRALELRRQQGGLYGKTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHV2</accession>
    <name>A0A8H6FHV2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008169</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156373.1">
        <property type="nucleotide sequence ID" value="XM_037299037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHV2"/>
    <dbReference type="GeneID" id="59336566"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="58"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="164" mass="17601" checksum="54EE5E2CDF3E609B" modified="2022-01-19" version="1">MPKRKSASAAAETYDADGGEAPRSKKAKGGEGKKEGGKKEGEGKKEGGGKKEGGGEFWELAGKRRVGISEFKGKTMINIREYYEKDGESLPGKKGISLPVEQFNTLIKLLPHIETVLAEKGQSVERPDYSGAGASAAMDEDGDEEDEEVEGNGKKNFEETSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI23</accession>
    <name>A0A8H6FI23_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS5m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_007035</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156565.1">
        <property type="nucleotide sequence ID" value="XM_037297930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI23"/>
    <dbReference type="GeneID" id="59335435"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000022">
        <property type="entry name" value="28S ribosomal protein S5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000041">
        <property type="entry name" value="37S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277:SF1">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="11">
        <location>
            <begin position="238"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6228923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="401" mass="45317" checksum="0027B6914AB22CF8" modified="2022-01-19" version="1">MSGPAPLRCLFSKSAAPYRCLRRNLHSSKQLHQRPDFPKIPTKAPRGAPRKDVARTVTTADDFKAYSEEDKRKLTTHFKYTPAQLAAIEAGEAAIDPKDLAEQAMMRDDPFAFEYYDDFATIHPVVDKPIRAPEENYDPKLRYKNEDEIAEDFVKFIEEMPEDATRLDYMKFMDNMRLTVGKEEAERKPVSSLAPEIPKGLESLKQPGIGKPGEVEIDPAMKRLMRQTGFSLDDIKRFRVKNLVVHRVVNQTKMGKIQSMYFLTVAGNGRGLLGIGEGKSAEAEDARRQAHYAAIRNVQPIPRYEDRTIYGDVKVKMGAVELELMTRPPGFGIRCQDKIFEMCRCAGISDLAARVTRSRNPMNTIKATFQALLSQKIPDEVARGLGKKLVDVRKVYYGGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI67</accession>
    <name>A0A8H6FI67_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156625.1">
        <property type="nucleotide sequence ID" value="XM_037297990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI67"/>
    <dbReference type="GeneID" id="59335497"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="124" mass="14189" checksum="CC7478543BC77BC7" modified="2022-01-19" version="1">MGNWSTEEEVVVLYYASREIRYCTIIEPLQHKCHTSVHKKASLTNRLRKLKNASQDAGLGQMQNTTINTGQARPWDRKVVDSWLVRMMEKGKLEKVLEFDGATAAIIEPDHDIDKFLDTMALNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIH0</accession>
    <name>A0A8H6FIH0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156755.1">
        <property type="nucleotide sequence ID" value="XM_037298120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIH0"/>
    <dbReference type="GeneID" id="59335627"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033167">
        <property type="term" value="C:ARC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018606">
        <property type="entry name" value="Arb1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09692">
        <property type="entry name" value="Arb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="643" mass="71650" checksum="2B9D7EB8D42BA215" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIN0</accession>
    <name>A0A8H6FIN0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156896.1">
        <property type="nucleotide sequence ID" value="XM_037298261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIN0"/>
    <dbReference type="GeneID" id="59335769"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="72" mass="8826" checksum="D3F68AD1EC4B6191" modified="2022-01-19" version="1">MSESVSANAVWQYDYIDHYRLDENIIGGFLDRKWGHYKYYVRRKGDEFRFWVPRALDKDEKNKLIELRKPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIS0</accession>
    <name>A0A8H6FIS0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156943.1">
        <property type="nucleotide sequence ID" value="XM_037298308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIS0"/>
    <dbReference type="GeneID" id="59335816"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="87" mass="9384" checksum="06890399B37B9EBF" modified="2022-01-19" version="1">MGGAETMAGIDVPAERSHGSYATHNTPLYTFGDKTAGDGQLHRCRDWNELRDFATENTACYRDSVGDIPLGEHFGYCDEGVDGLTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIV4</accession>
    <name>A0A8H6FIV4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007424</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GILT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156950.1">
        <property type="nucleotide sequence ID" value="XM_037298315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIV4"/>
    <dbReference type="GeneID" id="59335823"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF8">
        <property type="entry name" value="GAMMA-INTERFERON-INDUCIBLE LYSOSOMAL THIOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6229308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="274" mass="30460" checksum="BB0566B60BD54028" modified="2022-01-19" version="1">MDEKSAMEKRYGRKSPLPLYQKKHDSSFTAPSDISRLRSRPVVYITAIIFAFLIITYGLPVRLSTFNPQGSPETIRTQPAHAKSNRTGLVPLEAHVMSKCPDAKQCLEELVVPAMEQVVDMVNFRLSYIGSVDDNDTLHCMHGQTECLGNMLSLCASNLFPNNAKISLGFSTCLIMSYKRIPSRDLVQSCALEHGIPFEALNDCVSEEGKGLDLLEASIKRSQEAGVKKSCTVRVAEEIWCIMDDGKWTDCKAGHEVKDLVKEIESRYKPNATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJY3</accession>
    <name>A0A8H6FJY3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_004209</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157129.1">
        <property type="nucleotide sequence ID" value="XM_037295128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJY3"/>
    <dbReference type="GeneID" id="59332618"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000003">
        <property type="entry name" value="Histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Histidine kinase" evidence="16">
        <location>
            <begin position="409"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="17">
        <location>
            <begin position="714"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="676"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="876"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="930"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="979"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="883"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="936"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1001"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="14">
        <location>
            <position position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6229872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1030" mass="115368" checksum="F7C77361BE17FE47" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK03</accession>
    <name>A0A8H6FK03_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U6 snRNA phosphodiesterase</fullName>
            <ecNumber evidence="5">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">USB1</name>
        <name evidence="7" type="ORF">HO133_004298</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 2H phosphoesterase superfamily. USB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK03"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990838">
        <property type="term" value="F:poly(U)-specific exoribonuclease activity, producing 3' uridine cyclic phosphate ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034477">
        <property type="term" value="P:U6 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03040">
        <property type="entry name" value="USB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027521">
        <property type="entry name" value="Usb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522:SF3">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09749">
        <property type="entry name" value="HVSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="311" mass="34070" checksum="5DD85A3759AFC5D0" modified="2022-01-19" version="1">MPLVQYSDSESSSSDSPQPDRIHSKSQTSQGIKRKHSPTSHSVLPSLPDSFHDLYASTARVSNQDDPSLHAGRQRVTPHVQGNWPTHVYVDDAQSLQEHQVHSLLKSELGVELPLHISLSRPIVLLAHQRQPFIDSLTRAINKTGLSPFEVAVGGLEWVANYEKTRWFLVLKLERPPQNGLNKLLHLSNQTVTDFGQPPLYTDSLQSSTDGRSRKRQAGNGSRSKGITGAAASSSMTRSGPSTDIDMSSSFHISIGWTLGAPTHGLRERVDATGIDFQAIKVDINTVKAKIGNSITAISLATKIDTSRRMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKQ1</accession>
    <name>A0A8H6FKQ1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004657</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157574.1">
        <property type="nucleotide sequence ID" value="XM_037295573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKQ1"/>
    <dbReference type="GeneID" id="59333064"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="134" mass="14156" checksum="630764253902A6CC" modified="2022-01-19" version="1">MPDDGKLKSGVSSEHAVNVLSRRITGNIGAICQHSSICLLRSRFHAEETRGDVTVEGADSNYGSSDGQSGQFGPRECMESLGGGGYIDSILSLEVETNIARVYRDANALSIWGGKGGAEVLRPSENWVGAGAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKY3</accession>
    <name>A0A8H6FKY3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferroxidase</fullName>
            <ecNumber evidence="3">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004762</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the frataxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157677.1">
        <property type="nucleotide sequence ID" value="XM_037295676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKY3"/>
    <dbReference type="GeneID" id="59333168"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034986">
        <property type="term" value="F:iron chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.10">
        <property type="entry name" value="Frataxin/CyaY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017789">
        <property type="entry name" value="Frataxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002908">
        <property type="entry name" value="Frataxin/CyaY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036524">
        <property type="entry name" value="Frataxin/CyaY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020895">
        <property type="entry name" value="Frataxin_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03422">
        <property type="entry name" value="mito_frataxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821">
        <property type="entry name" value="FRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821:SF2">
        <property type="entry name" value="FRATAXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01491">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01219">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55387">
        <property type="entry name" value="Frataxin/Nqo15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01344">
        <property type="entry name" value="FRATAXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50810">
        <property type="entry name" value="FRATAXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0409">Iron storage</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6230420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="242" mass="26443" checksum="551B38CA0C32C648" modified="2022-01-19" version="1">MAHKAISISISLSLSKAPRRILPSLSLSHSQPRAPTSSSYPINPPRPSSFPTTQPHGKLHNGQLRLQPQPRTRSRTFTTGRPRRAAKAEAAEISLDEYHRISDRYIDGLVAQLEEMQEEREEVDVEYSVRAPPPPPDFSPGTFTSPTTGPTLRTYAGVLTLLFPPHGTYVLNKQPPNKQIWLSSPVSGPKRYDWVVRGADGDGAEKGEWVYLRDGSTLSGLLEEEVGVAPGEGEGEGEGDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKZ2</accession>
    <name>A0A8H6FKZ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004776</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157690.1">
        <property type="nucleotide sequence ID" value="XM_037295689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKZ2"/>
    <dbReference type="GeneID" id="59333182"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000140">
        <property type="term" value="F:acylglycerone-phosphate reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6230433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="271" mass="28971" checksum="25565D21E3A552D1" modified="2022-01-19" version="1">MADKKISSSKRTVLITGCSDGGLGAALAIAFHEAGLHVFATARNPSKMTQVASLGIETLTLDVQSDSSIADCVRKLSDLDILVNNAGAAYSMPASDLSIPKAKDIFDLNVWSYLAMTQAFLPLLLKSKGMISTYNASKAAMAMFSDSQRLELAPFGITVVDLKTGAVASNLIKNQKEATQASLPKGSIYEPAKEAVERTMRGDAFEDAGMPAQQWAGLVVQDLSRKRPPPNVWRGAKAGLAWVGTVLPFGMLDGMMKKMTGIDVVEQMVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL23</accession>
    <name>A0A8H6FL23_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157714.1">
        <property type="nucleotide sequence ID" value="XM_037295713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL23"/>
    <dbReference type="GeneID" id="59333206"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="141" mass="14978" checksum="2B2963A7BB8471CA" modified="2022-01-19" version="1">MVGGTAGVLEEDRQTEKNRHSTPSKSISSMSLNHPSKIDRHELAAANASMLVRVGSTIGYLAIDGNKKAIVVRWPAAMAYGIEAMASCSEQLRIFSNTSASKPPEVADSHRHACLVRSKAMYVATPLLSAAEAIAASLRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZG6</accession>
    <name>A0A8H6KZG6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157176.1">
        <property type="nucleotide sequence ID" value="XM_037295175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZG6"/>
    <dbReference type="GeneID" id="59332665"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="166" mass="18323" checksum="B3450496798798B3" modified="2022-01-19" version="1">MAGEKEDRADSSDEVEDGDGDDDGANYAYVTGEEGQWVQDESGDRPIVGASGSHGTAPPEPLRDHYYSSDLHRMHTKPDGSRSDDRDRQYGNNNRGSNSGEQDSHRYFETASEPRPSIIPRQQEPFASRLIPTMVVIEAGISTTAEPRGEEIRTAFSMHPTARPML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZN4</accession>
    <name>A0A8H6KZN4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase Rv2170-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157358.1">
        <property type="nucleotide sequence ID" value="XM_037295357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZN4"/>
    <dbReference type="GeneID" id="59332848"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053225">
        <property type="entry name" value="Acyl-CoA_N-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013653">
        <property type="entry name" value="GCN5-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20958">
        <property type="entry name" value="GLYCINE N-ACYLTRANSFERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20958:SF6">
        <property type="entry name" value="GLYCINE N-ACYLTRANSFERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08445">
        <property type="entry name" value="FR47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GCN5-related N-acetyltransferase Rv2170-like" evidence="2">
        <location>
            <begin position="279"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="403" mass="42283" checksum="F676EE453C0E8A0F" modified="2022-01-19" version="1">MTTTLPQTYIHSAPLATLAPHLLPHLPKSLPLLRRLQFHTASPSARIIATFPPPSSSSPPPTTTTNTAPPASFAAIWADRTRAPETECWIFSTFETLPPPPPTPSQRHEARAQCLALLAVTARLPATTPLTIIGSLNESLLRLIGGEEVGRLAPQKVVSRGTEHDDDDDDDTGTGGGGVIRGVSVPYCKWLIGPPGAEGGGGRSQGVGSLPPGYAFDELRGEDELKLTIARTEIPKTEETLAQLGCCGVRHTPGSASTATATATRTAADAREGEEGQGRGALVAWAFLGVDGSLTSLHVEPEHRGRGLAKAVSRRLFRSLADDASAMGFRAADGAGDGVGGYGRGEGWAHSDVAEENLGSAGVARAMGGKEGWRVRWVSVDLAAVVEERWTKLLGEEVVVRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZW4</accession>
    <name>A0A8H6KZW4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_004663</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
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        <text evidence="8">Interacts with MCM1.</text>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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            <dbReference type="PubMed" id="32504651"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">WasteWater1</strain>
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        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
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    <name>A0A8H6C6P0_9LECA</name>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037297630.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59335134"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020100">
        <property type="entry name" value="Glc-repressible_Grg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38789:SF1">
        <property type="entry name" value="GLUCOSE-REPRESSIBLE GENE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38789">
        <property type="entry name" value="REPRESSIBLE PROTEIN GRG1, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G14210)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11034">
        <property type="entry name" value="Grg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="71" mass="7139" checksum="00DCEDEF8E392FFC" modified="2022-01-19" version="1">MDAASNMAKSASDTAQSTFNSASESVKGTSEQASKEGNKNIAEDSDQSAGTRASAAKDAASNKVGEVKHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7B2</accession>
    <name>A0A8H6C7B2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147396.1">
        <property type="nucleotide sequence ID" value="XM_037297271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7B2"/>
    <dbReference type="GeneID" id="59334774"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40625:SF1">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE GLYCOGEN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40625">
        <property type="entry name" value="GTP-BINDING PROTEIN ESDC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034901618" evidence="2">
        <location>
            <begin position="18"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="603" mass="65876" checksum="C2BE2908551FAC2B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7F0</accession>
    <name>A0A8H6C7F0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006433</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147456.1">
        <property type="nucleotide sequence ID" value="XM_037297331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7F0"/>
    <dbReference type="GeneID" id="59334834"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000010">
        <property type="entry name" value="60S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_uL22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="187" mass="21165" checksum="0ECA11AE5A7BD0BA" modified="2022-01-19" version="1">MVRYAATTIEQGKSARAHGSYLRVSFKNTRETAQAINGWKLERALTYLGNVIDHKEAVPMRRYAGSTGRTAQGKQFGVSKARWPKKSAEFLLSLLKNAESNADTKGLDTGNLIVKHIQVNQAPKQRRRTYRAHGRINPYMSNPCHIELILTEGEEVVQKGPQVAEIEKKRLNSRQRGVRLRRAITQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C868</accession>
    <name>A0A8H6C868_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005788</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147874.1">
        <property type="nucleotide sequence ID" value="XM_037296693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C868"/>
    <dbReference type="GeneID" id="59334193"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030378">
        <property type="term" value="F:serine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018114">
        <property type="term" value="F:threonine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000005">
        <property type="entry name" value="Threonine dehydratase catabolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43050">
        <property type="entry name" value="SERINE / THREONINE RACEMASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43050:SF1">
        <property type="entry name" value="SERINE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="9">
        <location>
            <begin position="63"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="9">
        <location>
            <begin position="239"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6218439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="424" mass="45334" checksum="55E8C4C05134A0EA" modified="2022-01-19" version="1">MADLSRTLPLKRSSVQSAHERIKDHIQLTPVLTSTNLSNLASTPQTPEALVGTPFEGQRPARPRINVFFKCENFQRTGSFKIRGAFHALSRLSEEELAKGVVTHSSGNHAQALALAARTHGIPAHIVMPTISTPSKIAATQGYGANVYFSGSTSDEREAVVKDVIRVTGAVLVPPYDHPDIILGQGTMALELEEQAREWIMKDPALSVHHRGQQDVTNGHRNTSHTNGIHASSLVSEPGHLDAVIAPIGGGGMLSGIATALHGTGTYVFGAEPSFEGADDARRGLAAKKRVTTVKTLTIADGLRTPVGEIPWTIISDKTKMRGIHAVTEEQILQAMRLVLERMKLFVEPSAVVGLAVALYDEEFRSLVEKEGGEEGWDVGVVLSGGNTTVEAISKMFAVSEGKGERMEGKLGGQGERIAENVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C873</accession>
    <name>A0A8H6C873_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005892</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147978.1">
        <property type="nucleotide sequence ID" value="XM_037296797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C873"/>
    <dbReference type="GeneID" id="59334297"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF14">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="212"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="516"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="889"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="895"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="932"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="942" mass="105421" checksum="4C03020CA64F0FB9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8A9</accession>
    <name>A0A8H6C8A9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148177.1">
        <property type="nucleotide sequence ID" value="XM_037296195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8A9"/>
    <dbReference type="GeneID" id="59333690"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="375" mass="40134" checksum="DC1E5370199F3772" modified="2022-01-19" version="1">MTMVDFRRPDPYQLPHRKPLPPVPDISKRTDLRPLSKIIPDIGLPPSRQPPPTSTFSSYTRNGPKPPPTSSPSSQQYPNAIAKARRTPSQSTTSTSHSNTGYPPSRTPSNVSSVLSRNASTRSGASTTPGSYVALMRKQKATVWCDRSQHEDPRIAAQHKAAKIRAAREITGQAEGRTSTSGSMGSSSLGVRSKIRHHGLPKATGYSGANMVGGGVPMRLSANEVGDDGNHYDDADSGRRISGRSSGDNRWLSVDQQQRGNRYSQGSTPTGSGQGSSPNDDIPEMAETEETPVPGDQQQQGADYFAHDSGKGGSGGSSERENSFGNVGQMQAPKVEKESKTIDDLRRRGSVDERANTMGYMGGGRLFVANPDLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C907</accession>
    <name>A0A8H6C907_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148309.1">
        <property type="nucleotide sequence ID" value="XM_037296327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C907"/>
    <dbReference type="GeneID" id="59333823"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47771">
        <property type="entry name" value="LD27203P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47771:SF14">
        <property type="entry name" value="RH73259P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="6"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="589" mass="65536" checksum="0D572F9A9E0C6B2F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C985</accession>
    <name>A0A8H6C985_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HRDC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005138</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the exosome component 10/RRP6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148748.1">
        <property type="nucleotide sequence ID" value="XM_037296050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C985"/>
    <dbReference type="GeneID" id="59333544"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071040">
        <property type="term" value="P:nuclear polyadenylation-dependent antisense transcript catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06147">
        <property type="entry name" value="Rrp6p_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Exosome complex exonuclease Rrp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.80:FF:000001">
        <property type="entry name" value="Putative exosome component 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012588">
        <property type="entry name" value="Exosome-assoc_fac_Rrp6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045092">
        <property type="entry name" value="Rrp6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049559">
        <property type="entry name" value="Rrp6p-like_exo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124:SF47">
        <property type="entry name" value="EXOSOME COMPONENT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124">
        <property type="entry name" value="POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08066">
        <property type="entry name" value="PMC2NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="HRDC" evidence="10">
        <location>
            <begin position="451"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="634"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="646"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="727"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6219313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="816" mass="92370" checksum="B0903706C18377C6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9T6</accession>
    <name>A0A8H6C9T6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WDR59/RTC1-like RING zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004082</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149048.1">
        <property type="nucleotide sequence ID" value="XM_037295001.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332491"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="115"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="202"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="WDR59/RTC1-like RING zinc finger" evidence="5">
        <location>
            <begin position="1285"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="610"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="713"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1005"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1341"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="610"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="736"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1013"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1047"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1346"/>
            <end position="1358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1407" mass="155034" checksum="8943480D0A9BBAD6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAM1</accession>
    <name>A0A8H6CAM1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149179.1">
        <property type="nucleotide sequence ID" value="XM_037294491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAM1"/>
    <dbReference type="GeneID" id="59331980"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="26888" checksum="7E5F039841D108D9" modified="2022-01-19" version="1">MNAVSGNFTYPPQNSNLKFDFRDSIVASWVTDDFYATQGVLTLWYYITDAPANWTLSGVIEAVPANGSKSVALDVGTTSDFLGQFNINYNTNDGIQQKYDSAIFNVVHDLGEISQTMTWNLQALSTTSSGSPTPSKLQAASTHGSMTTSTTPSALTITGPSKTSYPNPHLAAAIGLGVGIPFGIAMICLIGFLIWKKTKSDRRRSQAASQTPATFVKGEKIMGELDAHQRHLELSVGRIDAELDASRSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAT9</accession>
    <name>A0A8H6CAT9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Protein phosphatase PP2A regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003286</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Phosphatase 2A affects a variety of biological processes in the cell such as transcription, cell cycle progression and cellular morphogenesis, and provides an initial identification of critical substrates for this phosphatase. The regulatory subunit may direct the catalytic subunit to distinct, albeit overlapping, subsets of substrates.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149590.1">
        <property type="nucleotide sequence ID" value="XM_037294209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAT9"/>
    <dbReference type="GeneID" id="59331697"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000174">
        <property type="entry name" value="Protein phosphatase PP2A regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000189">
        <property type="entry name" value="Protein phosphatase PP2A regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01025">
        <property type="entry name" value="PR55_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="405"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="409"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00055525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="493" mass="55568" checksum="C1B187B212B0F79B" modified="2022-01-19" version="1">MVAPEEPTQPTWKFTQCFGDKGDVEDITEADIISTVEFDHTGNYLATGDKGGRVVLFERNESKKTCEYKFHTEFQSHEPEFDYLKSLEIEEKINKIKWCRRQNASHYLLSTNDKTIKLWKVFEKSLKVVAENNLSHDLTPGGAIGGGGGPPRPNPNVSFRDASSLKLPRLTHHDTVVAAVPRRNYANAHAYHINSISVNSDGETFISSDDLRINLWNLNIQDQSFNIVDIKPTNMEELTEVITAAEFHPQSCNWFMYASSKGTIKLADMRENALCDQHAKQFEQEEDPSARSFFSEIISSISDVRFSHDGRYILSRDYLTVKIWDVNMERTPVKTIPIHEHLRPRLCDTYENDSIFDKFEVVFSGDAKNVMTGSYNNNFMIYPSDPERETEVVLQADKSAFKQKKIGVPTPINSSTSPTNGPSNKKGNSRAGSPAAGAGGQGGRMKKETDADQIDFNKKILHMSWHPFEDSIAIAATNNLFVFSALHEQSQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBE6</accession>
    <name>A0A8H6CBE6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AMP deaminase</fullName>
            <ecNumber evidence="4">3.5.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myoadenylate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_003302</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.6" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149606.1">
        <property type="nucleotide sequence ID" value="XM_037294225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBE6"/>
    <dbReference type="GeneID" id="59331713"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00663"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01319">
        <property type="entry name" value="AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.20:FF:000001">
        <property type="entry name" value="AMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000035">
        <property type="entry name" value="Probable amp deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01429">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="141"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="258"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="313"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="915"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="946"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="961"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1003"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1030"/>
            <end position="1071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6220171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1071" mass="122032" checksum="F683A3552E975CF2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC62</accession>
    <name>A0A8H6CC62_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150174.1">
        <property type="nucleotide sequence ID" value="XM_037294096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC62"/>
    <dbReference type="GeneID" id="59331584"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034456711" evidence="1">
        <location>
            <begin position="18"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="147" mass="15022" checksum="9EB8828625EEDF87" modified="2022-01-19" version="1">MHFSAVILAALSAGAHALPTADANIGGDLSSNLNPGPHNPAIGIFSNPSCEGKELQTPLSFPAAGCYPFDSAFDTVGINFGGDDNCGLNVYTDPNCGNFATHTLTGDINEFTCLSMSANGGPWKSLAPAECHPEAITDNPGVPEAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCG6</accession>
    <name>A0A8H6CCG6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003174</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150176.1">
        <property type="nucleotide sequence ID" value="XM_037294098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCG6"/>
    <dbReference type="GeneID" id="59331586"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF5">
        <property type="entry name" value="MEDIUM-CHAIN ACYL-COA LIGASE ACSF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="27"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="424"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="515" mass="56479" checksum="8C8B1C0DB4FEB05B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCK1</accession>
    <name>A0A8H6CCK1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Endosomal peripheral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_002503</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MON2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150258.1">
        <property type="nucleotide sequence ID" value="XM_037293429.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330916"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF333">
        <property type="entry name" value="PROTEIN MON2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034897995" description="Endosomal peripheral membrane protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="8">
        <location>
            <begin position="25"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HUS" evidence="6">
        <location>
            <begin position="222"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="7">
        <location>
            <begin position="999"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="618"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="618"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1693" mass="185497" checksum="420922A1C70325D9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCK6</accession>
    <name>A0A8H6CCK6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002660</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150414.1">
        <property type="nucleotide sequence ID" value="XM_037293585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCK6"/>
    <dbReference type="GeneID" id="59331072"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098771">
        <property type="term" value="P:inorganic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000005">
        <property type="entry name" value="Putative Cation diffusion facilitator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF11">
        <property type="entry name" value="CATION DIFFUSION FACILITATOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="7">
        <location>
            <begin position="161"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="447" mass="49865" checksum="9F6AC3DCA32DE57A" modified="2022-01-19" version="1">MVHKPLTNGHATSDYKPDDLEAAISASRRKPRFKDIVNAAVHDDFHGELKRKLKDGVDRDELEKYRKSISELSEIKDKKVRAFYEQQNERLNDWLEVDTLVMSMADDVLDSMNPQDADGDGVAEIGGALKTTKGDIDPLLPEDERERRKTGERRAKWAININVIANIILLTAKIIAAFASSSLSLIASLVDSALDLLCTLIIWTTNKLVQWKIRRLRHKFPVGRRRLEPLGILVFSIIMIVSFLQILQESVTKLLPGGPRDAATLPPVAIGALVATVVIKGIIWFGCIRVKTTQVQALAQDCKTDVVFNTLSLLFPLLGHQFSLWWLDPVGAALLSLFIIYDWAETCFENVARLTGSAVSPNVLKKVTFLAWRFAPVVDAYKTITAYHAGDGVWVEMDIMLKEETRLSRAHDIAETLQYCLEGLSEVDRAFVSVDYTSQGPTGHAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCN7</accession>
    <name>A0A8H6CCN7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase II subunit beta</fullName>
            <shortName evidence="4">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002548</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer composed of two alpha chains, one beta chain and one beta' chain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150303.1">
        <property type="nucleotide sequence ID" value="XM_037293474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCN7"/>
    <dbReference type="GeneID" id="59330961"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000003">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF39">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="283" mass="32864" checksum="3E1542AF33EF07CB" modified="2022-01-19" version="1">MVYYADEEHPRKRLKLWDKSAANLVPGSMEDFNSETDSDYTSYWRDWFISSRGNEYFCEIDEDYLTDRFNLTGLNTDVQYYQYALDLVTDVFDLDCDDDMREQIEKSARHLYGLVHARYITTTRGLAKMLEKYKKGDFGKCPRVMCNAHPLLPTGMADLAHVQSVKLYCGKCEDLYNPKSSRHANIDGAYFGTSFQNILFQVYPALIPEKTIERYQPRIFGFKVHAAAALERWQSGKREEMQERLKDVGMEKCFKEDVELEDDMEEDEEEVAGLDGADDVLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD50</accession>
    <name>A0A8H6CD50_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phenylalanine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phenylalanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_002223</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150803.1">
        <property type="nucleotide sequence ID" value="XM_037293150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD50"/>
    <dbReference type="GeneID" id="59330637"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.380:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000053">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="entry name" value="Ferrodoxin-fold anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00469">
        <property type="entry name" value="pheS_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="Anticodon-binding domain of PheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="16">
        <location>
            <begin position="152"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="17">
        <location>
            <begin position="366"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6221368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="471" mass="53816" checksum="CCA270162BB1C94F" modified="2022-01-19" version="1">MKLAATGRAVRLAWVERSGIELRHQSTNAPRTPSLEIDGKCYVTDDWTNVPSSILSYLPHKLQLQPSHPVSITRQVIESNFPSPTYTHYNDLHPIVTVAENFDSLGFPRDHPGRSRTDTYYINKDTVLRTHTSAHEAEIFRRNKSEGFTISADVYRRDAIDRSHYPVFHQMEGARMWDRRKVPGGDVAKAVRTDFERLPKHSLSIEDPNPTTHAERNPRQAEHHSPKEVEAIAAHLKRSLEGVVVELFSRARKAAIAAGHVDANALEPLRARWIEAYFPFTSPSWELEVFWQGDWLEVLGCGVTKQDVLINAGVPDQIGWAFGVGLERVAMLLFSIPDIRLFWSKDPRFLNQFSSKENVNTFVPFSKYPECYKDVSFWLRSSSSSTGGGMRSNAQDFHENDVMEVVREVGSDLVEDVRLVDNFQHPVSGRKSLCYRINYRSLERTLRNEEVNQLHESVRKQLVEKLGAELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD84</accession>
    <name>A0A8H6CD84_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002732</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150486.1">
        <property type="nucleotide sequence ID" value="XM_037293657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD84"/>
    <dbReference type="GeneID" id="59331144"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01389">
        <property type="entry name" value="HMG-box_ROX1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051356">
        <property type="entry name" value="SOX/SOX-like_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789">
        <property type="entry name" value="FI18025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789:SF2">
        <property type="entry name" value="FI18025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="220"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="220"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="122"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="283"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="502"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="504"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="528" mass="59488" checksum="F9E1208092A49AEB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDB2</accession>
    <name>A0A8H6CDB2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquinone biosynthesis O-methyltransferase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinol 3-O-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.64</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinone 3-O-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Polyprenyldihydroxybenzoate methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.114</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">COQ3</name>
        <name evidence="6" type="ORF">HO133_002222</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">O-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis. Catalyzes the 2 O-methylation of 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid into 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid. Also catalyzes the last step of ubiquinone biosynthesis by mediating methylation of 3-demethylubiquinone into ubiquinone. Also able to mediate the methylation of 3-demethylubiquinol into ubiquinol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-methionine = a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="2.1.1.114"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinone + S-adenosyl-L-methionine = a ubiquinone + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:81215"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.64" evidence="5"/>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EC" id="2.1.1.114" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDB2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061542">
        <property type="term" value="F:3-demethylubiquinol 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010420">
        <property type="term" value="F:polyprenyldihydroxybenzoate methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="282" mass="31730" checksum="3EA86D0AC7EE7104" modified="2022-01-19" version="1">MVIRPTTSRNLSRLLRIPTQCRSSSQSSSVNAAEVSHFNNLASTWWDPYGSSRLLHQMNPLRHDFISSCIESQPDPTSSTNRRYLDVGCGGGIFAESAARLLWTKSVVGVDPSSQVISVAKAHARQDPLLLEPGRLEYQNKAVEDLPIPRIPEEQFDVVSLFEVIEHINRPAPFLSSCLPFVKPGGWLILSTIARTWTSWLTTNVVAEEVVRLVPRGTHDWSKYINEQELRRFFRAQDGWGRDGGLKSQGCMYMPAVGWRMVPGGEKWGNYFLGVRKDSTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDX0</accession>
    <name>A0A8H6CDX0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Proteasome activator subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_002347</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BLM10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150926.1">
        <property type="nucleotide sequence ID" value="XM_037293273.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330760"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032430">
        <property type="entry name" value="Blm10_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032372">
        <property type="entry name" value="Blm10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055455">
        <property type="entry name" value="HEAT_PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035309">
        <property type="entry name" value="PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021843">
        <property type="entry name" value="PSME4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170:SF3">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16547">
        <property type="entry name" value="BLM10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23096">
        <property type="entry name" value="HEAT_PSME4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16507">
        <property type="entry name" value="HEAT_PSME4_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11919">
        <property type="entry name" value="PSME4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome activator Blm10 N-terminal" evidence="13">
        <location>
            <begin position="23"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator Blm10 middle HEAT repeats region" evidence="12">
        <location>
            <begin position="379"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4-like HEAT repeat-like" evidence="14">
        <location>
            <begin position="1363"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4 C-terminal" evidence="11">
        <location>
            <begin position="1876"/>
            <end position="1964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="915"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1851"/>
            <end position="1871"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1232"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="923"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1858"/>
            <end position="1871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6221491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1964" mass="222394" checksum="A4CE732BEEC54DA5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE45</accession>
    <name>A0A8H6CE45_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150994.1">
        <property type="nucleotide sequence ID" value="XM_037293341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE45"/>
    <dbReference type="GeneID" id="59330828"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034897553" description="HIT domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="39002" checksum="DE63769C6527DDBB" modified="2022-01-19" version="1">MSLATVGAAVLVLCIVAYAMSHATDSDVDLHDPEIFQERKNLYRNKRDDFRRNFRAMEELREWKAKNDDYDYVELRAAPDSVKLHDASQRASIFISKVWNADRTIARHSSSGMIPPNLVGKATGSKPFAKSDTWFSEFESHNPSSLILSESQDSYIIGNLASYDKRQYPEFKPGENTPEGQGFGHCLVISRKRIFNVVDPDATANHSAMLKEMKEHFITFWKTEDGSPKLLRRVRSTFDEQNTKLASNDRNAESCESLRSTLIKDFDMLSNSFLRCNPDDFEFAFHAYPANSVGHLHMHVFPKNSDLRKFSTKSHDWKTIPIEAVLEVEEEDPRALVERGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE68</accession>
    <name>A0A8H6CE68_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151188.1">
        <property type="nucleotide sequence ID" value="XM_037292649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE68"/>
    <dbReference type="GeneID" id="59330135"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052520">
        <property type="entry name" value="ATL_DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42942">
        <property type="entry name" value="6-O-METHYLGUANINE DNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42942:SF1">
        <property type="entry name" value="ALKYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="2">
        <location>
            <begin position="11"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="191" mass="20688" checksum="B04E4533DA469284" modified="2022-01-19" version="1">MARSDEAEWWFNAVYAAVQQVPRGRVTSYGHIARLLGRSERPRQVGVCLKHLPSSSANTNHHFHDANVPWQRVINAKGIISPRGPGGATRQAAFLGRGSYISEYLIPSFSVPNAELQDIFPEQKTLSTPKTCLVRPLKSNADSEVCAEGVTVGTGDLGELTVDLGAYGWFPQFLTSDGAEASESEEEEGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEE1</accession>
    <name>A0A8H6CEE1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="10">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_001677</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151144.1">
        <property type="nucleotide sequence ID" value="XM_037292605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEE1"/>
    <dbReference type="GeneID" id="59330091"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000006">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008854">
        <property type="entry name" value="PRK11892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="9 10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="11">
        <location>
            <begin position="52"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6221709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="378" mass="40709" checksum="64648AC41D0665ED" modified="2022-01-19" version="1">MVTSRLLRPASRLLASSRPLIAPTRSVLQPAAPLPAIVNRRKYATPSGVKEVAVRDALNEALAEELESNEKVFILGEEVAQYNGAYKVTKGLLDRFGEKRVIDSPITESGFAGLTVGAALAGLHPVCEFMTFNFAMQAIDQIINSAAKTHYMSGGIQPCNITFRGPNGFASGVAAQHSQDYSSWYGSIPGLKVVAPWSSEDAKGLLKAAIRDPNPVVVLENELLYGTPFPMSEAAQRDDFVLPFGKAKIERPGKDLTIVTLSRCVGQALTAAEQMKKDYGVEAEVINLRSVKPMDVEAIVKSVKKTGHLMAVESGFPSFGVGAEILALTMEYAFDYLDAPAVRITGAEVPTPYAQGLENMSFPSEDLIAKYGAKLLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CET0</accession>
    <name>A0A8H6CET0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151554.1">
        <property type="nucleotide sequence ID" value="XM_037292135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CET0"/>
    <dbReference type="GeneID" id="59329621"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="2">
        <location>
            <begin position="461"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="565" mass="61198" checksum="FCDD51157409A779" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEV2</accession>
    <name>A0A8H6CEV2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151533.1">
        <property type="nucleotide sequence ID" value="XM_037292114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEV2"/>
    <dbReference type="GeneID" id="59329600"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056396">
        <property type="entry name" value="HEAT_SCC3-SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF0">
        <property type="entry name" value="LD34181P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24571">
        <property type="entry name" value="HEAT_SCC3-SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08514">
        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="2">
        <location>
            <begin position="361"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="977"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="984"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1072"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1102"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1152" mass="126972" checksum="AD676599D2433ABA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEX5</accession>
    <name>A0A8H6CEX5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial oxaloacetate transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_001353</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151702.1">
        <property type="nucleotide sequence ID" value="XM_037292283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEX5"/>
    <dbReference type="GeneID" id="59329769"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051508">
        <property type="entry name" value="Mito_Carrier_Antiporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45928">
        <property type="entry name" value="RE38146P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45928:SF1">
        <property type="entry name" value="RE38146P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="2"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="107"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="208"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6222267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="33952" checksum="F8F118752D05D0F6" modified="2022-01-19" version="1">MSTTTGSFIAGGIAACGAVTATHPFETIKIRLQLQGELQAKKDAPRLYKGVFHGVSVILKNEGPKGLYRGIGAAYVYQMVLNGCRLGFYEPLRQSLTSAIFKDSKVQSLGINIFTGAASGMLGAAAGSPFFLVKTRLQSYSPFLPVGTQHGYKNTVDGFRKIHRAEGFKGLYRGIEAAIVRTGFGSSVQLPMYYFAKRRLIKHLDMQEGAALHLASSACSGFVVCCVMHPPDTIMSRMYNQTGNLYSGVFDCLIKTVKTEGLLAIYKGFFAHLARILPHTILTLSLAEQTNKYMRKFEDRILPATVKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEY5</accession>
    <name>A0A8H6CEY5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exosome complex component CSL4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001328</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151677.1">
        <property type="nucleotide sequence ID" value="XM_037292258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEY5"/>
    <dbReference type="GeneID" id="59329744"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019495">
        <property type="entry name" value="EXOSC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE CSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10447">
        <property type="entry name" value="EXOSC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0271">Exosome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exosome complex component CSL4 C-terminal" evidence="4">
        <location>
            <begin position="124"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="240" mass="25414" checksum="990696E8D41407EA" modified="2022-01-19" version="1">MAAPTSLPPIAYPGLPLGPSTAYTPGPGTHLHAPSSTIHASLAGRPVLTPQKPPASNPTISIPRLLPPPARAPVPSANVGNTNTLPRVGSVVLCRVVRVRTRQVDVVMLAVGREDGGGGEDVCGDEWQAVVRKEDVRATEKEKVVCGDGFRVGDVVRGVVLGTMSMWLFNDDGDVGGAMADVVQISLGDQSNYYLSTARNELGVIMARSEAGNTMVPISWKEFRDPITGERESRKVAKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF65</accession>
    <name>A0A8H6CF65_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151779.1">
        <property type="nucleotide sequence ID" value="XM_037292360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF65"/>
    <dbReference type="GeneID" id="59329846"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF21">
        <property type="entry name" value="ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="586"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="665"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1105"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1159"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="704"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1181"/>
            <end position="1192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1192" mass="132040" checksum="9B714703053E2FF3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF69</accession>
    <name>A0A8H6CF69_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001351</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151700.1">
        <property type="nucleotide sequence ID" value="XM_037292281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF69"/>
    <dbReference type="GeneID" id="59329767"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="122"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="365" mass="39980" checksum="FE84125A309D1C9A" modified="2022-01-19" version="1">MAGPLVVPPPPPGGPQLTQKTSEVIGTFRPTKQFKLSKPEASVTSLDFDDSGEFCLAACSDDSLLLYNAKEGRNIKTLMSQKYGAHLARFTHHATSIIYASTKEDDCIRYLSTHDNAFLRYFRGHTQPVTSLCLCPSADTFLSTSLDNTVRLWSLSSPSPQARLNLSTPYLAAYDPSATVIAVASASTHSILLYDLRNYDKPPFATFDMRPYLTAQQVQSPKPDWTRLEFSNDGKSLLLASNHPQGHLLLDAFDGTLRAHCKRSHAPTNLRAAPGMPGALGQGDVCFSADGRYLIGGSGGDRDAIVWDTQGQVEVETKEIKPMCGLPCKGRASVVEWNPRYNMCATADREVVFWLPDEHVGMKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFU4</accession>
    <name>A0A8H6CFU4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_000751</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152050.1">
        <property type="nucleotide sequence ID" value="XM_037291688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFU4"/>
    <dbReference type="GeneID" id="59329169"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF24">
        <property type="entry name" value="REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10820)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="14">
        <location>
            <begin position="172"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6222704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="477" mass="52498" checksum="EE715E77C689762C" modified="2022-01-19" version="1">MNVILWAHGIEWNGPIITKLEHLRNRSGTLAVVNMIPLVIMSGRNNPLIGWLNISYNTFNLMHRWFGRIVASLAVSHGVVEITSIVIRGQETHTPGWESFSETLKEVRFITFGLVALVSICTIILTSWTAFRHAFYETFLHIHIALVSLVMAALWIHLGGLQQMTYLKAVISTWVIERLIRLSSLIYRNVGRGGTNADVEILPGDAMRITLRPARPWTFKPGQHVFLTIPSVGLWTSHPFSVAWSDAPPRPLRPGDLEKGIRTSQPTAQTTDTLALPPTSVSLIIRRRAGFTDRLHRKALDGPHRRVSLPALVEGPYGGTHQALHSYGTVVLFAAGVGITHQVALARDLVAGHAAGTVAARRVLLAWVVRAPDHLEWIRPWMAAVLGMPGRRDVLRVQLFVTRPRCTAEIHSPSSTVRMFPGKPDVDALLGMEARAQVGAMAVCVCGPGGLGDDVRAAVRRRQGGGNVDFVEEGFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGH0</accession>
    <name>A0A8H6CGH0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GDP/GTP exchange factor Sec2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000989</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152284.1">
        <property type="nucleotide sequence ID" value="XM_037291922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGH0"/>
    <dbReference type="GeneID" id="59329407"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21044">
        <property type="entry name" value="Rab11BD_RAB3IP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009449">
        <property type="entry name" value="Sec2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430">
        <property type="entry name" value="RABIN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430:SF0">
        <property type="entry name" value="SEC2P DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25555">
        <property type="entry name" value="RAB3A-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06428">
        <property type="entry name" value="Sec2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144284">
        <property type="entry name" value="Sec2 N-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDP/GTP exchange factor Sec2 N-terminal" evidence="4">
        <location>
            <begin position="67"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="289"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="524"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="117"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="524"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="542"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="620"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="662"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="692"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="715" mass="78630" checksum="4D736580D264BB39" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH64</accession>
    <name>A0A8H6CH64_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152545.1">
        <property type="nucleotide sequence ID" value="XM_037291110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH64"/>
    <dbReference type="GeneID" id="59328589"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="49732" checksum="4E37767F211DFC1C" modified="2022-01-19" version="1">MAPFEPSEATLRIVLLPDDGPERTECCNTRKGGDILAGYLEVFSESLVGVNITVVFQGIIRTWRPSLNAQPDHNATAEYKFLSETQEIEATSSHRHRWEGLSVYRLPFAFTIPTELISSRSDVNPEYLTLPPTVREGQSYYGLATKQEYMQPRITYVITAVSTRFPRGTTSATKCRTKREVTVMPSIPASPPLQIEHFPHEYKTTCVKTLKRRVWNRPLGELTISAAEPKAFNISTSAPRASTVAAVKLLFQPCEAHTPAATPYEWRLTVKSHLKVRTFYSTTPFTQVPTKESVKSEPLAEMTSSCTYREIRQCDTLSWRLHRLSSAGTIASDTLATPWTTTLIVPVNASKALIPTFLNPLSARRYVLVLHITVDKIHHEAIVLEVPVQVICDPSQPATLSKRGASSETSRDGAEHQIAEGVGTMDLEDCHVPSLLDTTQPPLYAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIA4</accession>
    <name>A0A8H6CIA4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED18</name>
        <name evidence="9" type="ORF">HO133_010532</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIA4"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09637">
        <property type="entry name" value="Med18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="240" mass="26521" checksum="819374F6582C7B08" modified="2022-01-19" version="1">MHELLLFGTIPASRHTQLLNILSGISSMQPSPIDSEKHLVFKPNRPPGSVGSLKVGGAQDVNVQKSQMQAHTLGDLFYMQMVAEVDGREKGEGEERWVLQFRDLPEVARQRTVTSRLIADVRITSGDPMEFMKALDYSHVSTYTISGHRFTHHSASLLLYRVTLPANALKSDSTSDGLPQARLLDPSGTYILQASVRVQDGSKVETMTKGVNELLSLKETLKGVVELEIGDRLALDTRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIT9</accession>
    <name>A0A8H6CIT9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010833</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153318.1">
        <property type="nucleotide sequence ID" value="XM_037301684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIT9"/>
    <dbReference type="GeneID" id="59339223"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034873183" evidence="2">
        <location>
            <begin position="22"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="224" mass="22885" checksum="300A76301BE0653B" modified="2022-01-19" version="1">MLLQSRWLSSLLFIASAAAYAYPPAGLKPRYLATGTGISLPSTSPPPYPTSSANGTSVSTYPTSASTTATPTPTSSELFFLVAADTGTDLDGDYVSIGSDSSGNGLLVLYFAAGPNQVASFSTFNINADGTLENEFVGGIASIFAGSKYDSLHFENEVFVDEAGDTKSICEVVGGALTCQTGAATVFFVCPEQDIDGSLIGGTVEVGPTTQSGCTTLTLLAVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIU8</accession>
    <name>A0A8H6CIU8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MHYT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010525</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153011.1">
        <property type="nucleotide sequence ID" value="XM_037301377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIU8"/>
    <dbReference type="GeneID" id="59338915"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005330">
        <property type="entry name" value="MHYT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35152">
        <property type="entry name" value="DOMAIN SIGNALLING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G11310)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35152:SF1">
        <property type="entry name" value="DOMAIN SIGNALLING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G11310)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03707">
        <property type="entry name" value="MHYT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50924">
        <property type="entry name" value="MHYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="MHYT" evidence="3">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="627"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="751" mass="83849" checksum="C3EEF3823712257D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK92</accession>
    <name>A0A8H6CK92_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153971.1">
        <property type="nucleotide sequence ID" value="XM_037301154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK92"/>
    <dbReference type="GeneID" id="59338691"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054471">
        <property type="entry name" value="GPIID_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF14">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22939">
        <property type="entry name" value="WHD_GPIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="4">
        <location>
            <begin position="132"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase winged helix" evidence="3">
        <location>
            <begin position="412"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="671"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="705"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="739"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="832"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="865"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="988" mass="110923" checksum="40BCE279BE6BCAB3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKN2</accession>
    <name>A0A8H6CKN2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">INO80 complex subunit F domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153952.1">
        <property type="nucleotide sequence ID" value="XM_037301135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKN2"/>
    <dbReference type="GeneID" id="59338672"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056513">
        <property type="entry name" value="INO80F"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24245">
        <property type="entry name" value="INO80F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit F" evidence="4">
        <location>
            <begin position="98"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="148"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="334"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="192"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="336"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="391" mass="44277" checksum="D0B35F974D7C1DA4" modified="2022-01-19" version="1">MGQMTLVGHHAAMTMAHTYRITQLEATSYATTSQDSFHMDRAYVREKLELLRAGLPAAIGRILRQQQLLKQTANTVSPSTVAAGNVPPPLPPSVEAAYKKKCIELKRRLIEVDEDNDAKRLRIARLHQGIRKMRLERSYLLEALAKRQKKNGSSIDGFPVAYDEESEGSSEGPPTPNEKPLRSKRSHRRPVSSPPPILGPQFPRPIAPSQGHPTSAFDAPGNHPFRESSYQQAPTTNGHPGMHYQSHQSPYASNIGRDQQQQQLLPRAAPPLNAFEDFLENYVQARPNEYPARDQEELLQYARGAWDDPAKVEYRRVYEERYGHRFQEYMDSVNELEERERYERERERGYEQRGRAGRYEEREVERELPPPPPPPPAAGAGALGGFTSINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKX0</accession>
    <name>A0A8H6CKX0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010447</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154117.1">
        <property type="nucleotide sequence ID" value="XM_037301300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKX0"/>
    <dbReference type="GeneID" id="59338838"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF55">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G01820)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="57272" checksum="FB5D8D54C4CD76F2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL78</accession>
    <name>A0A8H6CL78_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dynein light intermediate chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_009569</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154278.1">
        <property type="nucleotide sequence ID" value="XM_037300430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL78"/>
    <dbReference type="GeneID" id="59337964"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035974">
        <property type="term" value="C:meiotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008467">
        <property type="entry name" value="Dynein1_light_intermed_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022780">
        <property type="entry name" value="Dynein_light_int_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688">
        <property type="entry name" value="DYNEIN LIGHT INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688:SF0">
        <property type="entry name" value="DYNEIN LIGHT INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05783">
        <property type="entry name" value="DLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="344"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="414"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="463"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="370"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="463"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6225569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="60038" checksum="A79FD82945E5BD91" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLI5</accession>
    <name>A0A8H6CLI5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 6A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_009704</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154413.1">
        <property type="nucleotide sequence ID" value="XM_037300565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLI5"/>
    <dbReference type="GeneID" id="59338099"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000009">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000076">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000037">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="245"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="12"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6225704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="465" mass="51970" checksum="DE9CC39EEC959117" modified="2022-01-19" version="1">MSTLEDLDDMEREEKEEKKDDGDKDGKKPDQDGDAEMKDADSDKKGEEKEEDIFDPEILHSSTRDIITRRRLLENDMRIMKSEFQRLTHEQAAMKEKIKDNLDKIENNRQLPYLVGNVVELLDLDVEKEAAEEGANIDLDATRIGKSAVIKTSTRQTIFLPLIGLVDHTALKPGDLIGVNKDSYLVLDTLPAEYDSRVKAMEVDEKPTEKYTDVGGLDKQIEELVEAIVWPMKEAERFKKIGIKAPKGALMYGPPGTGKTLLARACAAQTDATFLKLAGPQLVQMFIGDGAKLVRDCFALAKEKAPSIIFIDELDAVGTKRFDSEKSGDREVQRTMLELLNQLDGFASDDRVKVLAATNRVDVLDPALLRSGRLDRKIEFPLPNEEARAQILKIHSRKMTVDDAVNWPELARSTDEFGGAQLKAVCVEAGMIALRKGMVKIGHEHYVDAIAEVQAKKKDTVNFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM26</accession>
    <name>A0A8H6CM26_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7918 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154453.1">
        <property type="nucleotide sequence ID" value="XM_037300605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM26"/>
    <dbReference type="GeneID" id="59338141"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057678">
        <property type="entry name" value="DUF7918"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR EAF N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25534">
        <property type="entry name" value="DUF7918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="199"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="626" mass="70413" checksum="A2E19C3AD06839D9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMI8</accession>
    <name>A0A8H6CMI8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154766.1">
        <property type="nucleotide sequence ID" value="XM_037299940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMI8"/>
    <dbReference type="GeneID" id="59337474"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="282" mass="32304" checksum="F599EB276A4CE815" modified="2022-01-19" version="1">MGFERDSLLRRASRKDCADAQDKRTGELISDIELKRKEKQKALKLYEQRRMVQDQGAEIIVGFEPLLQKTQELQRLEETLQKKRSTVHDSEAEISDLRDRDTQGADNGLLLTVLTSMSTVQMKTAATLMGRMTIQQRASSSVKQLFQLQDSLAPNSHQNGHPYTHIVEAVGGSYFKPILAETNIIRKTLTAFRYVVTFLGYKLYINYEVGSDATSALPELDRTFDRKAVLQALRDKPPAGREAVWGRYIVKIDAEIEQRQEEVLQDIRDITEKSWTAEERRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMN3</accession>
    <name>A0A8H6CMN3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4185 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154902.1">
        <property type="nucleotide sequence ID" value="XM_037300076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMN3"/>
    <dbReference type="GeneID" id="59337610"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="453"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="54970" checksum="FCD6837E914BF244" modified="2022-01-19" version="1">MLASPDVPTEGLAALSAMPPENTITDNVTNASASSPGNNTNDGPHTAADTQIEAGEPSITHKTGTDDPDHEADIGPAIYPPALENMEVLGYIKDKNGIDYFRDIGCSASIHDEVYFVFGDTFCKNSAGKSVGTTSNTIAYVEDRTNFLESEYRELSNNARVKAFVPLNDREIRFEEKNENARVVFRMFGGIADIGVVGVVWFQKSIKYENGEESYRGVGQARLSTYSDGSILVERLPSLLFGPNEPRIGSFSTLFYKGHVYLWGDGPKGQIMLARVDQYETALRDRYEYWSGSDWVPRWHDAVPVLHDVQHGAIIHTNLFGKEKPFVFVGVNKQADSMVQIGAAAEVQGPFALAAVCQATSIKSDKEHKCCIYPHIYPHLWASNVPKRELMITWSESPPGGVIAAKLKFKIDEVAAVKEAEERKRAAEERKRAAEEQETRRLASIAKEQEANFEYGFVSDESDDPRPRRDRSEGRLKTGYVLRVVRTSSDHPTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN26</accession>
    <name>A0A8H6CN26_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009398</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155085.1">
        <property type="nucleotide sequence ID" value="XM_037300259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN26"/>
    <dbReference type="GeneID" id="59337793"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6226532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="11216" checksum="71F138BE3E6FC285" modified="2022-01-19" version="1">MSSPRPSSPTSPPASAGVSSGRNLSPPGGPKTAIRRKAAADRADKVANARPTSTRAAGAGGSSSTMLRLYTDESPGLKVDPVVVLVLSLGFIFSVVALHIIAKVTRKFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN29</accession>
    <name>A0A8H6CN29_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bet v1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009425</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155112.1">
        <property type="nucleotide sequence ID" value="XM_037300286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN29"/>
    <dbReference type="GeneID" id="59337820"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39332">
        <property type="entry name" value="BLL4707 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39332:SF7">
        <property type="entry name" value="SRPBCC FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="16929" checksum="14A8CC3DA3098AF5" modified="2022-01-19" version="1">MTSRIPTSTSITESSVIAAPLAQVWHLIKLQDFHHFWTKLDKSHHIKGVSHETDVVKWTFKDGTEQEVKQEEHSSIDHFITYSVISSTPALTYSSVISTIRCYAVTTGPSQGSTFVQWSAHFSSDADAGVIEDAKFKRKEALADLAHAAVGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNC5</accession>
    <name>A0A8H6CNC5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipid droplet-associated hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. LDAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154957.1">
        <property type="nucleotide sequence ID" value="XM_037300131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNC5"/>
    <dbReference type="GeneID" id="59337665"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019363">
        <property type="entry name" value="LDAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390:SF0">
        <property type="entry name" value="LIPID DROPLET-ASSOCIATED HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10230">
        <property type="entry name" value="LIDHydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="41186" checksum="E5CF141AAC851899" modified="2022-01-19" version="1">MSMSRDPLLGDFSFEKSDKICYEPSTDSTDRKTQDYLIYFLPGNPGLIQYYQPFLSRLYTLLSSSATTESSRFHICGHTLRGFESAQDGKKTKAPRYPLGLEQQIESQEQLLYNHIKSHRERTGNNPKVILMGHSVGCYILLELIQQHRGKIEGEGEEDFDLIGGILLFPTITHIAKSPLGMVFGKILQFPYFPEIVGTIAKALASLVPESFLHRLVKLVTRFPEYAATTTTSFIKSPMGVRQALHLAKDEMTKITDDQWDEEVWGAATSKGTNNRDTANSNLTFYWGNKDRWVATHTRDALIAARGHLSNSQTESGSEYWKPVMMVDNEGMPHDFCTTLRNSHSVAGKVKGWVDGIIEAHSKEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNH2</accession>
    <name>A0A8H6CNH2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009345</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155032.1">
        <property type="nucleotide sequence ID" value="XM_037300206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNH2"/>
    <dbReference type="GeneID" id="59337740"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000297">
        <property type="term" value="F:spermine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF38">
        <property type="entry name" value="POLYAMINE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="77"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="141"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="58"/>
            <end position="483"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226479.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="521" mass="56463" checksum="6777D4F692FEB6DE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNU7</accession>
    <name>A0A8H6CNU7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155151.1">
        <property type="nucleotide sequence ID" value="XM_037299150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNU7"/>
    <dbReference type="GeneID" id="59336680"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="163" mass="18143" checksum="C50F27DDCFF63690" modified="2022-01-19" version="1">MQVAKCVYFTAHLQLCAPVLTTSLRLPTSSPALFSSETFNVTHEFLLNSSKELERPASSSQILKFNDYGAQIIADYDVYSSLQSAARDVTTHASEEVVGWRERQYGSVDVRLLPHPGPSMTWGMWKEAIRAIAQFVTHYQSLDMDFDVVQARLVVGTGVLTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPS6</accession>
    <name>A0A8H6CPS6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein PAN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Actin cytoskeleton-regulatory complex protein pan1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HO133_008809</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the PAN1 actin cytoskeleton-regulatory complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037299672.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337204"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein PAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013182">
        <property type="entry name" value="DUF1720"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF173">
        <property type="entry name" value="ACTIN CYTOSKELETON-REGULATORY COMPLEX PROTEIN PAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF1720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EH" evidence="20">
        <location>
            <begin position="159"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="20">
        <location>
            <begin position="439"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="472"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="258"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="307"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
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            <begin position="564"/>
            <end position="627"/>
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    </feature>
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        <location>
            <begin position="775"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="953"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="699"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="63"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
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        </location>
    </feature>
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        <location>
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            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
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        </location>
    </feature>
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    </feature>
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        <location>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="933"/>
        </location>
    </feature>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1028"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1073"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="1156"/>
            <end position="1166"/>
        </location>
    </feature>
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        <location>
            <begin position="1170"/>
            <end position="1190"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1191"/>
            <end position="1209"/>
        </location>
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        <location>
            <begin position="1210"/>
            <end position="1232"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1252"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="1399"/>
            <end position="1440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009351"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011159"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015110"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020728"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF6227365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1484" mass="160698" checksum="775ADCF975A76110" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPV0</accession>
    <name>A0A8H6CPV0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Patatin-like phospholipase domain-containing protein</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008482</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Lipid hydrolase.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Probable lipid hydrolase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PLPL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155349.1">
        <property type="nucleotide sequence ID" value="XM_037299348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPV0"/>
    <dbReference type="GeneID" id="59336878"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07230">
        <property type="entry name" value="Pat_TGL4-5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF10">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="8">
        <location>
            <begin position="225"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="580"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="774"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="5">
        <location>
            <begin position="256"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="580"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="595"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="623"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="682"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="750"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="785"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6227041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="794" mass="87700" checksum="CE4A2F7F69F80F81" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ47</accession>
    <name>A0A8H6CQ47_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155747.1">
        <property type="nucleotide sequence ID" value="XM_037299746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ47"/>
    <dbReference type="GeneID" id="59337278"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009081">
        <property type="term" value="P:branched-chain amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000010">
        <property type="entry name" value="Branched-chain amino acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000004">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825:SF2">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE 3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6227439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="378" mass="41173" checksum="8AF8F9EE318CA389" modified="2022-01-19" version="1">MPFPPPPVESIDWDNIGFKVRDVNGHIESYYSVNTGKWSEPQFVADPYLRIHGMAPALNYGEQAFEGIKTFRTPDDQIAIFRTMANAKRMAHSAGFVAIPPVPETHFVKCCHLAVGLNAEHVPPHRTGASMYIRPLVYGSSAHLGLTPPEEFTFVVFVVPVGVFHGVHPVDALILEEFDRAAPEGTGSAKLGGNYGPVLKWSERAKKDGFGITLHLDSRTKTEVDEFSTSGFVGVHKEGEKYTVVVPDSKSVIKSVTSDSVCEIAKSLGWKVECRSIKYDEISTFTEIMAAGTAAALVPIKSITMRSRNDKFIYQGGSDKPGPACEKLLTTLKSIQQGKIKDPFGWLDYVKEPKGFPSINGASTDGVNGVDGSVDELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQY9</accession>
    <name>A0A8H6CQY9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">DNA repair protein REV1</fullName>
            <ecNumber evidence="14">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_007985</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="14"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156189.1">
        <property type="nucleotide sequence ID" value="XM_037298853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQY9"/>
    <dbReference type="GeneID" id="59336382"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017125">
        <property type="term" value="F:deoxycytidyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17719">
        <property type="entry name" value="BRCT_Rev1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01701">
        <property type="entry name" value="PolY_Rev1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000001">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000040">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000011">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1280">
        <property type="entry name" value="DNA repair protein Rev1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
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    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025527">
        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053848">
        <property type="entry name" value="IMS_HHH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012112">
        <property type="entry name" value="REV1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031991">
        <property type="entry name" value="Rev1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038401">
        <property type="entry name" value="Rev1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21999">
        <property type="entry name" value="IMS_HHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16727">
        <property type="entry name" value="REV1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14377">
        <property type="entry name" value="UBM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036573">
        <property type="entry name" value="REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 14" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRCT" evidence="17">
        <location>
            <begin position="61"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="UmuC" evidence="18">
        <location>
            <begin position="475"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="197"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="377"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="848"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="945"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1019"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1109"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="852"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1025"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1109"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="574"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR036573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR036573-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6228255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1259" mass="138790" checksum="874E17F697A69E27" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRF5</accession>
    <name>A0A8H6CRF5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008040</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156244.1">
        <property type="nucleotide sequence ID" value="XM_037298908.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336437"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16482">
        <property type="entry name" value="RING-H2_UBR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19673">
        <property type="entry name" value="UBR-box_UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase UBR2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003769">
        <property type="entry name" value="ClpS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF24">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02617">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="11">
        <location>
            <begin position="161"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="8">
        <location>
            <begin position="161"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="478"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="525"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1372"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="490"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="525"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="555"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="578"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6228310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2442" mass="275583" checksum="DEE9AB68CD205211" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRS1</accession>
    <name>A0A8H6CRS1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphotransferase</fullName>
            <ecNumber evidence="11">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_008088</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the hexokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156292.1">
        <property type="nucleotide sequence ID" value="XM_037298956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRS1"/>
    <dbReference type="GeneID" id="59336485"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000092">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000004">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="12">
        <location>
            <begin position="27"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="13">
        <location>
            <begin position="229"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6228358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="489" mass="54121" checksum="55DFF7D351C330E4" modified="2022-01-19" version="1">MVGLGPRRQPSRKGSMADVPDDLLEQIKYLENVFTVDQAKLKEVTNHFVKELEKGNVEGGSIPMNPTWVMGFPDGHETGTFLALDMGGTNLRVCEIILLEEKGEFDIIQSKYRMPEELKTGESEELWEYIADCIQQFIEYHHEVEDLEKLDKIPLGFTFSFPATQNYIDNGILQRWTKGFDISGVEGKDVAPLFEAALAKRKLPIRLSALINDTTGTLIASAYTDDTMRIGCIFGTGCNAAYMENCGSVPKIASYNLPPDMPIAINCEYGAFDNEHIVLPRTPYDVVIDNDSPRPGQQAFEKMIAGLYLGELFRLVLVDLHDNKSCKVFQGQNISKLRKAYTLDSSFLSMIEEDPFENLSETGDLFHRELGIKTNKPELELCRRLAELIGTRAARLSACGVAAICKKKNMETCHVGADGSVFNKYPHFKARGAVALREILDWKVGEKDPISILAAEDGSGVGAALIAALTIKRVKAGNLAGIRNVEGMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS10</accession>
    <name>A0A8H6CS10_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008264</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156467.1">
        <property type="nucleotide sequence ID" value="XM_037299131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS10"/>
    <dbReference type="GeneID" id="59336661"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="41"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="351" mass="38900" checksum="4ACC5FADF39E863B" modified="2022-01-19" version="1">MATPDAAEIQYQRSHIQDDRSKNIVISHIICIIIAIIAVALRLASRRLCKAAILADDFVCFAALIFAIGEVTGGWMCVHYGGGKHAILLKNPETFAKWVITTEIFYNAAITTIKLSILLLYRRLFPLPQLKIILWSVGCFVISCWLTITLLTIFQCHPIRAAWDLSVKGQCLRLNVAYIVLGTCNTITDMVALCLPMPLIWRLHTDKSRKMQLIGVFSLGALTCAVSLYRIPKMAQLSLSDAPWSDVDAAVWGVVEVNLAILSACLPTFPALFNWRSVHRRNTPPGGADPKSWGSASYPALIPDERRAGFRSGSGYAHKDALKMEDWSEETKGAERATVCIERRGPSDEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6G4</accession>
    <name>A0A8H6F6G4_9LECA</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">HO133_006813</name>
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    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>OSLEUM clade</taxon>
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            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146910.1">
        <property type="nucleotide sequence ID" value="XM_037297709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6G4"/>
    <dbReference type="GeneID" id="59335213"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034679123" evidence="1">
        <location>
            <begin position="22"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="212" mass="22708" checksum="94D3B4934D6E7BD1" modified="2022-01-19" version="1">MALQSFVTIAILFNWGSAVSAYPQAQDLGEAQKIYPRTVSGDVHQISVPTNSTTAPPLPPPPVGVIECFAPGVGRKTTDVDGCRPTLNHFRTFPNYRLIQDFMEGRYPKLPSKPPYAIHQIRSTCAVQIASGDSYTIDDFSFEQARALATEILEVCQDHGGCGGFAPIGHGVGWKVVVIGFTLPPGPPDRTGLLEEVGSGNGTSMPVRIAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7J9</accession>
    <name>A0A8H6F7J9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pathway-specific nitrogen regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006681</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
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            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="A0A8H6F7J9"/>
    <dbReference type="GeneID" id="59335082"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="159"/>
        </location>
    </feature>
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            <end position="367"/>
        </location>
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        <location>
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            <end position="531"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
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        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
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            <end position="95"/>
        </location>
    </feature>
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        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
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            <end position="146"/>
        </location>
    </feature>
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        <location>
            <begin position="490"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="59659" checksum="6C47360028561724" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7M1</accession>
    <name>A0A8H6F7M1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>OSLEUM clade</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147707.1">
        <property type="nucleotide sequence ID" value="XM_037297133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7M1"/>
    <dbReference type="GeneID" id="59334635"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="566" mass="62282" checksum="CF5DF0F4479ED4E1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7S3</accession>
    <name>A0A8H6F7S3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006631</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147239.1">
        <property type="nucleotide sequence ID" value="XM_037297529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7S3"/>
    <dbReference type="GeneID" id="59335032"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="239"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="433"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6217804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="585" mass="64204" checksum="FD3756D053C3F898" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F892</accession>
    <name>A0A8H6F892_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148275.1">
        <property type="nucleotide sequence ID" value="XM_037296293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F892"/>
    <dbReference type="GeneID" id="59333789"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="260" mass="29796" checksum="20057EB66039163C" modified="2022-01-19" version="1">MADQNSSDWKFHPLASDFERRGPFRYFDLPTEIRCQILDLALAPGHSPRGGAVSGMDPRAAGMGLLQVSHRMQDEAEAVLCSGKNIFYLPLCKMGQFYDWAYRTMRTIPPVKKLDCAFDMRDVAENTSAMVQRMKAENRLDVVGDDFERLSRSEKAELLHRESFLDLLYVWTDKIDAFIVLDLDLLRLDLSNCRCLSGCCRLGQGLLENLDFDQTYAEYPQRIEIIGALKEEENLWRAAVRRGSHVPLERVFFIDAPEHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8I8</accession>
    <name>A0A8H6F8I8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148575.1">
        <property type="nucleotide sequence ID" value="XM_037295877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8I8"/>
    <dbReference type="GeneID" id="59333371"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="521" mass="59024" checksum="A1E56B8D403C4597" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8L8</accession>
    <name>A0A8H6F8L8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005565</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148456.1">
        <property type="nucleotide sequence ID" value="XM_037296474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8L8"/>
    <dbReference type="GeneID" id="59333971"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052374">
        <property type="entry name" value="SERAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182:SF2">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034031453" description="DUF676 domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="DUF676" evidence="9">
        <location>
            <begin position="14"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="961" mass="109278" checksum="0C94C87551DE58BC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8S0</accession>
    <name>A0A8H6F8S0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN FLCN/SMCR8-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148621.1">
        <property type="nucleotide sequence ID" value="XM_037295923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8S0"/>
    <dbReference type="GeneID" id="59333417"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021713">
        <property type="entry name" value="Folliculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441:SF2">
        <property type="entry name" value="FOLLICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441">
        <property type="entry name" value="FOLLICULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="2">
        <location>
            <begin position="190"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="390" mass="42392" checksum="B9F178C3B0E5C65E" modified="2022-01-19" version="1">MDIILSLSHFCENDGPTSILCTQIKPVECLSCYPASISSSVGDLDATHRGPAPSAPAFLSSSNRSSLDFGGTPESAPNSASTSPEIQPATLGAHRQNAPRPLNPLSRFPVAGSNGKGCESCTISLPEEISSKIPPGAPGSPKADGSGRNGSPILRFKESLQIYRDQQTDSDSEEDHEGPIRLNQQASPESFASDTSASFYHKHTLSYISTSSPTDPNTYSIIRRAAIRTLSGEQLPRGQTAGELFFGTPLDGWTIAYKFRLSDPYARGGHRKYALIALASSETRAYQVTTFIWEHFRRIAENIITRTAQKIKENEGDRGDHEDITKEHCIPVSSFLTGRTIDPDGYPRQYGAARMRARGLTEMVGDDKFFAELHLEFVGMLRDLMGGYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8U0</accession>
    <name>A0A8H6F8U0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycerol-3-phosphate phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005412</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148304.1">
        <property type="nucleotide sequence ID" value="XM_037296322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8U0"/>
    <dbReference type="GeneID" id="59333818"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050308">
        <property type="term" value="F:sugar-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051806">
        <property type="entry name" value="HAD-like_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481">
        <property type="entry name" value="FRUCTOSE-1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481:SF4">
        <property type="entry name" value="GLYCEROL-1-PHOSPHATE PHOSPHOHYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="27024" checksum="26DF59EBE143E6BB" modified="2022-01-19" version="1">MALDQGNGSFTGQTESHAFAGFLFDVDGTIINTTDAVTKHWQRIGAELGVDPDVILRNSRGRRSIDTLRLKLGMSVSLPGPPQCHIDIYATDVRHIESLIPKLYGSGVTEIRGARPLLKSMGEANAPWALVTSCTRTLLSGWLDLLALPSPPASVAAEDVAAGKPDPACYNLGRERIGLGERARVLVAEDAPAGVKAGKAAGCMVLGLATTHGVDQLRDAGADWIIEDLTCVKVAGKGDDGWEISIEKMWVQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F921</accession>
    <name>A0A8H6F921_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Multicopper oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004149</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149115.1">
        <property type="nucleotide sequence ID" value="XM_037295068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F921"/>
    <dbReference type="GeneID" id="59332558"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13854">
        <property type="entry name" value="CuRO_1_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13880">
        <property type="entry name" value="CuRO_2_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13901">
        <property type="entry name" value="CuRO_3_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000021">
        <property type="entry name" value="Extracellular dihydrogeodin oxidase/laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000038">
        <property type="entry name" value="Extracellular dihydrogeodin oxidase/laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF145">
        <property type="entry name" value="LCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="9">
        <location>
            <begin position="79"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="206"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="444"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="63425" checksum="5E4A3A985A12BBA4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9C5</accession>
    <name>A0A8H6F9C5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003626</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149236.1">
        <property type="nucleotide sequence ID" value="XM_037294548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9C5"/>
    <dbReference type="GeneID" id="59332037"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16536">
        <property type="entry name" value="RING-HC_RNF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000512">
        <property type="entry name" value="RING finger domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039739">
        <property type="entry name" value="MAG2/RNF10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983">
        <property type="entry name" value="RING FINGER 10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="192"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="411"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="591"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="647"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="691"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="728"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="431"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="592"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="647"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="728"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="756"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="765" mass="84443" checksum="AAA68B7DCC739EF1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9F1</accession>
    <name>A0A8H6F9F1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acyl-protein thioesterase 1</fullName>
            <ecNumber evidence="3">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004057</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149023.1">
        <property type="nucleotide sequence ID" value="XM_037294976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9F1"/>
    <dbReference type="GeneID" id="59332466"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000010">
        <property type="entry name" value="Acyl-protein thioesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="13">
        <location>
            <begin position="6"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6219588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="229" mass="25208" checksum="F4AD09125232DD65" modified="2022-01-19" version="1">MATAAPLIVPALKKHTATVIWAHGLGDSGAGWMPIAENFRRRGRFSECAFVFPNAPNIPVTVNFGMEMPGWYDITTFGDLAQAHDQPGILKSRDYFTSLIKAEIEKGIPSSRIVLGGFSQGGAISLFTGISCPDKLAGIFGLSSYLLLHNKIKDYVKSESANKNTPIFMGHGDVDPLVKHEWGLDTSKTLREMGWIVDFRTYKGLAHSADPKEIDDLQKYLEERLPPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAC4</accession>
    <name>A0A8H6FAC4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150510.1">
        <property type="nucleotide sequence ID" value="XM_037293681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAC4"/>
    <dbReference type="GeneID" id="59331168"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="99" mass="10391" checksum="078C58EA80B51A40" modified="2022-01-19" version="1">MSRYASSEGVLDPTPPSLNATDPGMLPLGISIGRKYVGAAEVARATYQMGKATASPYVPVLGYMSLVCPEPIGNVHSDAVRVCKNHTGSTDISTEAEKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAD7</accession>
    <name>A0A8H6FAD7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002501</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150256.1">
        <property type="nucleotide sequence ID" value="XM_037293427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAD7"/>
    <dbReference type="GeneID" id="59330914"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="447" mass="52074" checksum="317E0999AAC5FFF5" modified="2022-01-19" version="1">MPRYLVLRRSGVHRAACFALYRAILRQKHKLQLNEHHQHLFRKPIRAVFKRNSKLQSPPQISTALQLGYETLDGLNDGVRQQRLLNRILELASPASTSAQPEDLPSSPQTKIAPAQKTPRRHAARYPGARRTLDRPFFHLSGRRHVPKLINANRVPFLRLKKPQPPFLSRVIRDTVKTREHRILRAARLTSELPTAADEDEWDEILDEQFESDCRGPEVHPWQHEVQRALDSNHKLQVEAIQKRADISANMYTIQEQEKALAEEEKLRIRDEKHKATKARRLARRGLTESEVQERLYPQIEDKEVAKQGQEEARQPKMKQEWRLRGGKYKTSDELRRIKEASLRPKTDAEIAKIKEARARRKEEESEKKAEKLNRKQENASFWEQKLNKAKDSTNKRLGSEKQAKPPKSGVVRRNDYESIQPQPEIPPLLEELVESRKAAVWHQVHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAR9</accession>
    <name>A0A8H6FAR9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-1,3-mannosyltransferase CMT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002604</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150359.1">
        <property type="nucleotide sequence ID" value="XM_037293530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAR9"/>
    <dbReference type="GeneID" id="59331017"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021047">
        <property type="entry name" value="Mannosyltransferase_CMT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34144:SF5">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE CMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34144">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11735">
        <property type="entry name" value="CAP59_mtransfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="51126" checksum="C509BAE020614041" modified="2022-01-19" version="1">MAAKDSKFDGCFMSEANGPGQAHAYDWRKLRIFPNLPQSDHEQSALQSCSKVKGIESEPGRTGKETETTLGPVLEDDYIQQWQNGTKAHDMDALSPGEVAKCLREIMGEANSTAWRIQCSASIAARYDSLQVTTEHDGRIQYLFALDLYQTIPVLPTLLGSIIQTIRFLGPKRCAVSFVEGRSTDGTYQVLAALRDEVERLGAVFYMSTSDVSPQDGKQDRFEGLAFLRNLALAPLVMEKAKFSPDAQVIFLNDVYLCPHDILELLYQQKMQHATMTCGMDWSHGGAIFYDIYVARSIVGETFWQIAQDGGWQFSDNLFWADRQTRAQYLQRQPFQVYACWNGGAVISARPIMERRIRFRRNVEAECYMGEPTLFAKDLWRLGLGRIQVVPTVNVGYDNRTKEAKDLHGRVEDPLNMTSGDLQTELVQWQESPPSLVKCMAQFHEPYWVPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAW9</accession>
    <name>A0A8H6FAW9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150583.1">
        <property type="nucleotide sequence ID" value="XM_037292930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAW9"/>
    <dbReference type="GeneID" id="59330416"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051706">
        <property type="entry name" value="Glycosyltransferase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385:SF15">
        <property type="entry name" value="INOSITOL PHOSPHOCERAMIDE MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="414" mass="46765" checksum="2D77CB28A62CF95D" modified="2022-01-19" version="1">MPSIISPFTDQHKNIAFDRASRMRSLRHRTLSSTIALPLVLLVCFVFYTLKTPTKESSPFLFRLQPGHHLPISPLHSAAAPKARQISPAELMARPPPHSGPHMPKLLHQSWSTTILPSKFEQWSLSCRDMNPDFEWVLWTDEDNRRLVEKYAPEFLVKYDGLKSEIYRADAHNISSSSQSGSTSIQNPTHTPTSDAAPISTFPTTSYNAGPQTAFLARMGADNSKKHSIPNAWLASTPSHPFWLLPLNLVSKGTKPYGDWPEAVTGPDALFYLVNEYLREYRSDNDYGASLDEFLLKSELKEMYVRSLGEKNIRASAEVGMHQLVLLGKEMIFPYWWGEKELEGVCKAGEEGFDPETCKDVLDVQALGSWSITYWSHSWDQEGGHDEGHLSAMGSWSFRGARMLRRATNRKRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBW1</accession>
    <name>A0A8H6FBW1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152011.1">
        <property type="nucleotide sequence ID" value="XM_037291649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBW1"/>
    <dbReference type="GeneID" id="59329130"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="77" mass="9087" checksum="58082F8A63BB05CB" modified="2022-01-19" version="1">MTSSLNALDHNEHAFHLYDDNTAGFMESQLIALIFRNQRNDGVMEGQLKEDTKYQDQFQKMARDRDLVYNESKMNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBW3</accession>
    <name>A0A8H6FBW3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152073.1">
        <property type="nucleotide sequence ID" value="XM_037291711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBW3"/>
    <dbReference type="GeneID" id="59329192"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02883">
        <property type="entry name" value="NUDIX_Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27071" checksum="0E33EB5D3A367990" modified="2022-01-19" version="1">MSQKAGLYQEHTNIPKAHLPIVGNWDSANTTIGAGVAIFHLASSRVVLCYHSTKNYYFLPKGRRDASEDTGSGAEREGFEESGYRNRLLPLPIPTRQPKAHNPSPSSAATTPFVTEPVWTQLVPVTRTAQYILFWYIAETVPPDLEVSLSAQEKEQGMAYQYPPKFSPEMTLKERMEMEREGYEPIRHENTGVDAEEALYESYLMPVEEAIEKLGRGISADVVRKGWEAIQLKHKMEDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBW5</accession>
    <name>A0A8H6FBW5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151618.1">
        <property type="nucleotide sequence ID" value="XM_037292199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBW5"/>
    <dbReference type="GeneID" id="59329685"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="454" mass="48888" checksum="5571D35280ACA1F1" modified="2022-01-19" version="1">MAANSRKVVVFSGGSAANSLVDVFNEVIEKNNCALTYVIPISDNGGSSSELIRVFGGPGIGDVRSRLVRLIPQTHVHILNLFNHRLPPSSDLAASEFLTLLTGTSHLYHSVPTPQAMLIRSILSHLHLEILKRNRPPTSTFNFQSASIGNLFLTGARLFSGSFESAIYLLAIMGGVDESKTAVLPAIISNFSHHISAGLADGSVITGQNAISHPSEPTALNSNIDHANPLADLNDATIEDANLPGSLPTLRQPNIHFSKGSEEPLPARIQRLWYINPYGQEMRPSPNPKVLEAINFAEGLIYSIGSLYTSIVPSLILQGVGAAIARSGGPRFKILILNGSLDRETGGFSASDFIAAVARAGEESRGITFNHGEDVEKEIWARYVTHLIHLEGEGVPRVDKEELGKWGVECVRLYGRKAEGGTARYDGRALGQALGAILGRREKGEKSRRNTLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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            <taxon>Lecanoromycetidae</taxon>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037301471.1"/>
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    <dbReference type="GeneID" id="59339009"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR000504">
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    </dbReference>
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        <property type="entry name" value="MKI67 FHA DOMAIN-INTERACTING NUCLEOLAR PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="521"/>
        </location>
    </feature>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="814" mass="86240" checksum="19DD61FD40D4BA04" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDH3</accession>
    <name>A0A8H6FDH3_9LECA</name>
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        <submittedName>
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        </submittedName>
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    </gene>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <end position="494"/>
        </location>
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            <end position="226"/>
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            <begin position="479"/>
            <end position="489"/>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="589" mass="63561" checksum="178CF4ED77DBB6E2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDJ8</accession>
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        </submittedName>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6224405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153465.1">
        <property type="nucleotide sequence ID" value="XM_037301831.1"/>
    </dbReference>
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        </location>
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        <location>
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        <location>
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        <location>
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        <location>
            <begin position="291"/>
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        </location>
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        <location>
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        <location>
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        </location>
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        <location>
            <begin position="593"/>
            <end position="604"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="732" mass="78695" checksum="A88045241E84DFA3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDR4</accession>
    <name>A0A8H6FDR4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
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            <taxon>Pezizomycotina</taxon>
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            <taxon>OSLEUM clade</taxon>
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            <taxon>Lecanorales</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
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</entry>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
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    </evidence>
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    <reference evidence="7 8" key="1">
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                <person name="Walston R.F."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
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            <name>pyridoxal 5'-phosphate</name>
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    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037298210.1"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003961">
        <property type="term" value="F:O-acetylhomoserine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071269">
        <property type="term" value="P:L-homocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000035">
        <property type="entry name" value="O-succinylhomoserine sulfhydrylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43797">
        <property type="entry name" value="HOMOCYSTEINE/CYSTEINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43797:SF2">
        <property type="entry name" value="HOMOCYSTEINE_CYSTEINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="463"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="476"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="530" mass="57229" checksum="53F6E2F45C1BCD94" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIW0</accession>
    <name>A0A8H6FIW0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="2">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007477</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157003.1">
        <property type="nucleotide sequence ID" value="XM_037298368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIW0"/>
    <dbReference type="GeneID" id="59335876"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF26">
        <property type="entry name" value="FAD-BINDING 8 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034257445" description="ferric-chelate reductase (NADPH)" evidence="7">
        <location>
            <begin position="19"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="8">
        <location>
            <begin position="26"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6229361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="323" mass="36389" checksum="CDD50F4C987E65E6" modified="2022-01-19" version="1">MYIYISAALFLSTSVVQCVSIVWRNGTFRHGPARAFVTYAEGSVKISIRLSRPLTIQAGQYINLWMPSASFSSFLQSHPFVVTSWSPEEQIQLELFMEPRRGLTRELLYHAQARERGAKFSSRWVLFSGPYGISALVGEYESILMIVIGFGMAALLPYLSQMIHRYQTRKIYTRRIRVIWQVQRIGKQGRCIHTNKTKLTNVKTALAMQPLLNSALKGDSLDKILSISIFSTSLKEEEVSFGRRGIVYRGSADVTGILRDEIAVRNTENQYRDIEAAQIEAGHIREVTGKLQREKGKMLVMVSGISAQRRVGTTAGLVVGVYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK26</accession>
    <name>A0A8H6FK26_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Steroid 5-alpha reductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004337</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157256.1">
        <property type="nucleotide sequence ID" value="XM_037295255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK26"/>
    <dbReference type="GeneID" id="59332745"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010721">
        <property type="entry name" value="UstE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251:SF15">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE (DUF1295)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06966">
        <property type="entry name" value="DUF1295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="38590" checksum="D2A6A17321B06F85" modified="2022-01-19" version="1">MAEVHALDDYYLAITLLITIAYQLFFFSIAFSLKFDKLTDFAGGTNFVVLAIITLAFSGHHNARQIVDSLFIMIWGARLSGFLLFRIIKTGKDDRFDDKRDKFFPFLGFWVFQMFWVWTVSLPVTILNSPNVTKYRQPDFGTGRDIAGVILFTIGFLMESVSDIQKYRFRSRPENKGKVCDVGLFVWTRHPNYFGEIIIQFSIFMIAVSPAAYGYVHGGAYSALYASILGAFFLTILLMFVSGLPLQERPGAKKRYESGNHWESFSEYTERTSILIPFPPQLYVRMPIILKRTIFLELPLYVFDPAKHADQGKVQERQAEEGAADSGNEQTQDGHAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK28</accession>
    <name>A0A8H6FK28_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157195.1">
        <property type="nucleotide sequence ID" value="XM_037295194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK28"/>
    <dbReference type="GeneID" id="59332684"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018860">
        <property type="entry name" value="APC_suCDC26"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10471">
        <property type="entry name" value="ANAPC_CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="79" mass="8506" checksum="8D4315DFEF8932AD" modified="2022-01-19" version="1">MLRRKPTTITLTTEDVAAYDDARAATNDRQENVKPTDGDGKVDPNDELKPLPGDRPRVRSGVGSASGAGMGREERIVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK32</accession>
    <name>A0A8H6FK32_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-lactamase/transpeptidase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004284</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157204.1">
        <property type="nucleotide sequence ID" value="XM_037295203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK32"/>
    <dbReference type="GeneID" id="59332693"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050491">
        <property type="entry name" value="AmpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021860">
        <property type="entry name" value="Peptidase_S12_Pab87-rel_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46825:SF14">
        <property type="entry name" value="BETA-LACTAMASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46825">
        <property type="entry name" value="D-ALANYL-D-ALANINE-CARBOXYPEPTIDASE/ENDOPEPTIDASE AMPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11954">
        <property type="entry name" value="DUF3471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="2">
        <location>
            <begin position="22"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S12 Pab87-related C-terminal" evidence="3">
        <location>
            <begin position="421"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6229947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="59836" checksum="61AB8186B3DECA10" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKK1</accession>
    <name>A0A8H6FKK1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157502.1">
        <property type="nucleotide sequence ID" value="XM_037295501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKK1"/>
    <dbReference type="GeneID" id="59332992"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662:SF11">
        <property type="entry name" value="WSC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WSC" evidence="2">
        <location>
            <begin position="403"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="547" mass="58720" checksum="10236BD895017BC6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKL0</accession>
    <name>A0A8H6FKL0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157511.1">
        <property type="nucleotide sequence ID" value="XM_037295510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKL0"/>
    <dbReference type="GeneID" id="59333001"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF76">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="23"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="474" mass="52481" checksum="0E99280F0C712583" modified="2022-01-19" version="1">MHRQTLPIDQLGAWARLNNVEFNGVKIMASQGDKGSGLVTTAERSIDNPLLMTIPNDLVLSLENVWVYAKSDKHLRQVLEATGDYSRTARGAILIFLLLQVTNGATGGSIGVSNPFSEYVKFLPLRVPLPTTWNESERAIVTGTSLEAALSAKLNGLDRELNLLKEKTSSIDWCQKCWWDAASGTLTLDDWKTVDAMYRSRALDLPGTGHSMVPCIDMANHASGGSTSALYETDGDGNAILVLREGKDLAPNDEVTITYGDEKGACEMLFSYGFIESTVKSARELFLDLSIPDDDPLKLAKKAVAQSAPGFRLFLRGDSTDWESSFVWLLCVNEEDGLEIKLLQKNDGERELQASWKDKEITDMSNLVKLLKLEPLWDVFALRAIITCQNRVEQQLFRLEGSKNSVDELLIIGEVDNDIRANAMQLRHLEEILLLHAYGDFETKKSQILDSPVVQQYLGATMASNPGVSEDDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKM7</accession>
    <name>A0A8H6FKM7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157532.1">
        <property type="nucleotide sequence ID" value="XM_037295531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKM7"/>
    <dbReference type="GeneID" id="59333022"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08570">
        <property type="entry name" value="GDPD_YPL206cp_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805:SF1">
        <property type="entry name" value="GP-PDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="2">
        <location>
            <begin position="60"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="360" mass="41232" checksum="828CA1B5F3525BF4" modified="2022-01-19" version="1">MMAAVKPIIQKISPIPVPPFLLDDKFYDTLAEAPSLDMNVARPQPSWTFARKRPSGRKMPQAIAHRGYKAKHPENTMGAFRGAIKAKAHAMETDIHLTKDDVVVLSHDATLKRCFGKDEKIIDCSWDYISQQRTLAAPHEPMPRFKDLLEFLASPGSENVWLLLDIKLDNDAENVMRLIAETVQSVPPHPLKPWNQRLVLGCWAVKFLPLCAEHLPTFPITHIGFSIPYARKFLSVPNVSFNMLQKTLMLPVFGARFVRDVKAKGRPLYVWTVNEEDMMRWSINREVDGVITDDPKRFLEVCDEWEQGKRKITISWGQWMMAAWINFMVLVFGTIFWWKYSSSTGKGKPTQRKAVEEGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL04</accession>
    <name>A0A8H6FL04_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RlpA-like protein double-psi beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004840</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157753.1">
        <property type="nucleotide sequence ID" value="XM_037295752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL04"/>
    <dbReference type="GeneID" id="59333246"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF28">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034187090" description="RlpA-like protein double-psi beta-barrel domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="18296" checksum="9356BEAB80EF2200" modified="2022-01-19" version="1">MKAQTLALGLALSAPLASAYQGKMTNYQVSAGLTSCGTTHADSEMVVALSLSLMQQGWPGNPNDNPKCNTYISIWNPDTQVLTQNVKVVDSCRGCAMYDIDASDAVFAAVADPALDHGDGWEIVDWGGNGVGGKRDLGLETLTVGPVGKREPHHPHAKVAREEKGDGDDSQMAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL12</accession>
    <name>A0A8H6FL12_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Geranylgeranyl transferase type-2 subunit alpha</fullName>
            <ecNumber evidence="6">2.5.1.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Geranylgeranyl transferase type II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004830</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to cysteines occuring in specific C-terminal amino acid sequences.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157743.1">
        <property type="nucleotide sequence ID" value="XM_037295742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL12"/>
    <dbReference type="GeneID" id="59333236"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF2">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6230486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="562" mass="64696" checksum="1B47E50AD5FE682C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL15</accession>
    <name>A0A8H6FL15_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004808</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157722.1">
        <property type="nucleotide sequence ID" value="XM_037295721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL15"/>
    <dbReference type="GeneID" id="59333214"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398:SF4">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="826"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="258"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="328"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="528"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="982"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="373"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="558"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="678"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="985"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1007"/>
            <end position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6230465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1039" mass="114581" checksum="2604B29FE71F14F6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY26</accession>
    <name>A0A8H6KY26_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8004 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156585.1">
        <property type="nucleotide sequence ID" value="XM_037297950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY26"/>
    <dbReference type="GeneID" id="59335455"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058317">
        <property type="entry name" value="DUF8004"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39601">
        <property type="entry name" value="CHORIOGENIN HMINOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39601:SF2">
        <property type="entry name" value="CHORIOGENIN HMINOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26013">
        <property type="entry name" value="DUF8004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8004" evidence="2">
        <location>
            <begin position="327"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="698" mass="79205" checksum="F5277957A04B09C0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY39</accession>
    <name>A0A8H6KY39_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CorA-like transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156600.1">
        <property type="nucleotide sequence ID" value="XM_037297965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY39"/>
    <dbReference type="GeneID" id="59335470"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058257">
        <property type="entry name" value="CorA-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26616">
        <property type="entry name" value="CorA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="CorA-like transporter" evidence="2">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="466" mass="53675" checksum="26DDE3E90346BBD7" modified="2022-01-19" version="1">MRLKVEDREIFSPDERQSNVEVWLGRAPHTYLGHDEENITAFKEILANIRDADVDVFIFLRQEYSWGRLLITEDTFGKILDSCNAFPEFNDHVSSFAFKTSDCDEHFATCDRRVHVSAQAGQEDHNCYELCYLLRYPARHGRKSGYPFSLRQTAVYQRFDDVRKTSKWIFVQAPDDFSDELRTSLSSGNQERSPKDIHRCAFALAEQEWRDYICYLDAELRVLEEKALFVDVDVYSECDYLVAFADSQKLQRLRRRLVKCKEVLDCCLEIALRCQTHWQDLCSRGMLESGEDNAELGSYISRIKTHKRGIEAIQEHTEGIATLLSQILNYRNENMIVKSNEALNKNSDAMREIAIAAKADNELISVLIGKSQNDSHTVKVLTYIALIYLPASLVAEIFNSNLIQTTNGEPEAVGTHLVLSRSFWLYPSITLGLMVLTFVPIFGLLFNDRRLRRLPLKLLGNNKERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYG4</accession>
    <name>A0A8H6KYG4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156795.1">
        <property type="nucleotide sequence ID" value="XM_037298160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYG4"/>
    <dbReference type="GeneID" id="59335667"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="115" mass="12985" checksum="E54C0BA21D468B96" modified="2022-01-19" version="1">MRVPLETVSRQLAIQQMARTYDDDAIRVIVLDSTLHIFACNVTQIHLRGRPKDEAVRLPNLNGLGVSSTAPQATHEDNLLLDASQIELLPDDKEMTFTVKTKPQLYTARFRSLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYQ3</accession>
    <name>A0A8H6KYQ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Beta-lactamase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156930.1">
        <property type="nucleotide sequence ID" value="XM_037298295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYQ3"/>
    <dbReference type="GeneID" id="59335803"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050789">
        <property type="entry name" value="Diverse_Enzym_Activities"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283:SF3">
        <property type="entry name" value="BETA-LACTAMASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G07500)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283">
        <property type="entry name" value="BETA-LACTAMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="1">
        <location>
            <begin position="25"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase-related" evidence="1">
        <location>
            <begin position="218"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="418" mass="46161" checksum="01A1EA8BDA3AC71E" modified="2022-01-19" version="1">MTRTLSDSAKHGIRTSMDDVTADPHKIPGCVAVVVDRDGKTLFSHASGKKGIDTKEPMSLDAVFWIASCTKMIGGIAAMQLVEQGRLALDDADLVERYCPELKTIRILKGIDENCKAETVAKKNRITLRMLLNHTAGFEYTFFNDTVRLYGQPAGIDEFDCHPSGVLGQPLTFEPAFCTSAARRAKSSSRHRLGIRSIPPIFHHSSHSPHPTNPTRQVGIDWAGTLIERATSMSLNEYFQTHIFAPLDLHHITMFPTPQMKANLAHMHQKTPGHVRARDHLYRRPLIATPAEVPGIYNSAGAGCFGRPTDYAQILAALLNGGTSPATDNRILTEESVEEMFTNQIPAFPDFGRKGVRTAKPTYTNDIPDLYHQPEEQPQGWGLTFMLTIHEAATGRGKNTAWWAGELIYSMVVGEEGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZL1</accession>
    <name>A0A8H6KZL1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_004361</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man(7)GlcNAc(2)-PP-dolichol to produce Man(8)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29535"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19518"/>
            <dbReference type="Rhea" id="RHEA-COMP:19519"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132517"/>
            <dbReference type="ChEBI" id="CHEBI:132519"/>
            <dbReference type="EC" id="2.4.1.260"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29536"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157280.1">
        <property type="nucleotide sequence ID" value="XM_037295279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZL1"/>
    <dbReference type="GeneID" id="59332769"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052917">
        <property type="term" value="F:dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF1">
        <property type="entry name" value="DOL-P-MAN:MAN(7)GLCNAC(2)-PP-DOL ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="368"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6230023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="590" mass="66758" checksum="F8347D54CAA7CC66" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZX6</accession>
    <name>A0A8H6KZX6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157672.1">
        <property type="nucleotide sequence ID" value="XM_037295671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZX6"/>
    <dbReference type="GeneID" id="59333163"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF108">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="286" mass="32307" checksum="BEA86A1432AF9234" modified="2022-01-19" version="1">MVQNAITLLIAGYETTSGMFSFLFIMLLKKPRALKAAQNEVDKVIGTGPITIDHLNKLPYIAACMRETLQLWPTALESASSHVKPEKMLDEAFQKLPPRSWKNFDLRASGPTYVMKYRRTLSTNPIGFSFHATLRDHLYPVELAGRLWGHEEPHKHDVKDKKVNGDSETEKNPLQILYGSDAGTCATRIAEKGLSDVAGNHVFDDFDRWREDSMRPGIDMAFDHQNDITAQSDGLDVEFRDSYRVSDLRQDVSEAIFKSTKPLTGPGVPQKRHLELQLPSSMTYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5V7</accession>
    <name>A0A8H6C5V7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_006567</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>deamido-NAD(+) + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147175.1">
        <property type="nucleotide sequence ID" value="XM_037297465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5V7"/>
    <dbReference type="GeneID" id="59334968"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07570">
        <property type="entry name" value="GAT_Gln-NAD-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000036">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000003">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6217740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="706" mass="78989" checksum="A4553AD791C343A5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C651</accession>
    <name>A0A8H6C651_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_006779</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147112.1">
        <property type="nucleotide sequence ID" value="XM_037297676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C651"/>
    <dbReference type="GeneID" id="59335180"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="41"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="304"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="345"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6217677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="415" mass="46012" checksum="74DABED09B8ABF3E" modified="2022-01-19" version="1">MSGPPSMPGPPPGLNLHDDQGGKVLGTMIALIALASTFVILRLTSRHLARAGFWWDDVLIIVALLLAIAPCICSIVAVKHVGFGKHIYVFGKAGAMEAATGYFHTLYFFQLFFTLATGATKLTILAFYRRIFPIAELRLILIVMTTVVVMYTTAVSTLIIFQCRPIRKFWEFELPGYCINPKNNLAVSGSVNTLLDFMIVCLPVPLLWRLRTSTRQKSILTGIFLTAGFVCIVSIVRIISFSRADPSDVTWNFVWVSIWSAVEPIAGILGACLPSLRPLLTLLLDNNAYRSFAATASQTFQSTTTTTSSSVLKSNQSSRKNRDPTTFARLKEEHDGNAPKTQRLPWRDPKGPRVYDHGMDGHNVTVYGGRGQQADGDGVEMERMSDVEPPRGVIRVKTEIFLSSSKRLDYNDRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6T4</accession>
    <name>A0A8H6C6T4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006452</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147475.1">
        <property type="nucleotide sequence ID" value="XM_037297350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6T4"/>
    <dbReference type="GeneID" id="59334853"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="373" mass="43449" checksum="5B465C3BDAE54569" modified="2022-01-19" version="1">MTARYSPPLKPIRLSHAPAPQSFLRRRSRWLYGLLALVLLYLCRSSIPLLPHRTPIHEPSLRYKNVDWSRYAYSQYATSSPYLCNAVMVFEALSRLGSKADRILLYPQEWDLEIESTSDRDSQLLVIARDRYDVKLLPVVIPKADDDAWNGSFTKFMAWEQTQYDRVLHLDSDVTILKQLDELFMLPSAPVAMLRAYWRLPSVRQLTSLFILFEPDEMEAQQLMVAASPNVRQKNEYDMEILNKFYGDSAMVLPHRQFGLLSGEFRVDDHRNYLGNTHERWDPERVLREASLVHFSDWPIPKPWIMWPHNMIQDEMPKCKLGIGAGGDDCRDKKAWLGLYDDFRKRRKDICALLSAPAPEWHPPPRNTTEASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Y6</accession>
    <name>A0A8H6C6Y6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SprT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006376</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147399.1">
        <property type="nucleotide sequence ID" value="XM_037297274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Y6"/>
    <dbReference type="GeneID" id="59334777"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044245">
        <property type="entry name" value="Spartan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006640">
        <property type="entry name" value="SprT-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21220">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE SPRTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21220:SF0">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE SPRTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10263">
        <property type="entry name" value="SprT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SprT-like" evidence="2">
        <location>
            <begin position="308"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="718" mass="80826" checksum="D48FFF2931AD70C6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C773</accession>
    <name>A0A8H6C773_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Photolyase/cryptochrome alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147653.1">
        <property type="nucleotide sequence ID" value="XM_037297079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C773"/>
    <dbReference type="GeneID" id="59334581"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF18">
        <property type="entry name" value="SI:CH1073-390K14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0274">FAD</keyword>
    <keyword evidence="2 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="6">
        <location>
            <begin position="47"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="221"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="296"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="342"/>
            <end position="349"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="440"/>
            <end position="442"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="532" mass="61541" checksum="0B64CA86D043C52D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7D8</accession>
    <name>A0A8H6C7D8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147439.1">
        <property type="nucleotide sequence ID" value="XM_037297314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7D8"/>
    <dbReference type="GeneID" id="59334817"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034229249" evidence="1">
        <location>
            <begin position="18"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="217" mass="22521" checksum="44F7FF8B0CBCD4FC" modified="2022-01-19" version="1">MLSLIAAAVCLIGSVLTQTPPSYTLAVTNQTLGLSYGSVSVEAGETLPYKVPSTTPTLSSTLPLNETYIAIIVDTTANLSSPIPADLVWFQQDVTFSSAGIGSANTSAQVPYVAPSSVGHAYLALLYTQPPGFIIPSNFPYNDTFRVAFNVSRVAVDFHSPLLAANWFVLGSNCSAPSYTASSGYGYGASTGFASGTHGPWAKKTGAVYANGAAGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7M4</accession>
    <name>A0A8H6C7M4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005701</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147789.1">
        <property type="nucleotide sequence ID" value="XM_037296608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7M4"/>
    <dbReference type="GeneID" id="59334106"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000374">
        <property type="entry name" value="3-oxoacyl-(Acyl-carrier-protein) reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107">
        <property type="entry name" value="NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107:SF7">
        <property type="entry name" value="RE15974P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="256" mass="27114" checksum="AABAF7939E19542D" modified="2022-01-19" version="1">MALSLEGKVAIVTGASRSRGIGAQIAYELAKRGAKVVVTFTSPSSEKSVDELVKRIEQLQNGSAAAKVKADLRQVDAPEKIVQATRSAFGDHIDILVNNAGCEMVKGLGDISADDFSYVYNLNVRAVLLMSKVALPHLRAPGRIINVSSVGARLGLKDLSLYCSSKAAVEGMTRCFAAELGPQGHTVNTVNPGPVPTDVLDGIPKELVEMHKKMTPVEGRLGTTDDIAQIVGFLSEESSRWITGQAISASGGFSMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7W0</accession>
    <name>A0A8H6C7W0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147709.1">
        <property type="nucleotide sequence ID" value="XM_037297135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7W0"/>
    <dbReference type="GeneID" id="59334637"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="246" mass="27008" checksum="C25658309B6E5747" modified="2022-01-19" version="1">MKLFKKFVKLPENAKNAKSFSACRTFDADLRRINSCVEALGILGLQGTGGIDSAGEIVAPRDRRKLRIILYAPLSKFENDTIADYKNNKPVAIQEDTDVEYEPGPSNPEPPQKRPRTGEIGLMSSMGIQTRTRNLGDGWYMAYNSTTSIIPTQLGAANLEVFYTKVVDSAAKQISTTVNATQNLAFNINGLSLRLSSPAPISWTWIINFAADMLDNVSTDFAILFNGEAYSAYWEIAAVTAVLTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C856</accession>
    <name>A0A8H6C856_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phosphoglucomutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_005975</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148059.1">
        <property type="nucleotide sequence ID" value="XM_037296878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C856"/>
    <dbReference type="GeneID" id="59334380"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05799">
        <property type="entry name" value="PGM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000035">
        <property type="entry name" value="Pgm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="44"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="14">
        <location>
            <begin position="210"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="15">
        <location>
            <begin position="325"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="12">
        <location>
            <begin position="529"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6218624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="586" mass="65078" checksum="62F11793CBAF4B35" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8W4</accession>
    <name>A0A8H6C8W4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148449.1">
        <property type="nucleotide sequence ID" value="XM_037296467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8W4"/>
    <dbReference type="GeneID" id="59333964"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="110" mass="12340" checksum="5E50C80942AC6090" modified="2022-01-19" version="1">MPHSALDQRFGPLPQQAQSTTPLKQAAKQEQEREKQCAKEKVAKKSKAINSNDRDKTGLRSIFRQRIAMQIGQEASLRLRNASLLDGNKDAAVFSKRVTTCLSQDEVFEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C900</accession>
    <name>A0A8H6C900_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005614</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148505.1">
        <property type="nucleotide sequence ID" value="XM_037296523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C900"/>
    <dbReference type="GeneID" id="59334020"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="56756" checksum="7C2A2758B7F4A5D0" modified="2022-01-19" version="1">MALLDDVGSMHFFAIGLLLYTIYWALHPRFLSPLKEIPGPWYAVYTKWWLVWKTVKGVRAKTIHELHLKYGPWVRIAPNEVSTCDPQAIVPIYGVNSAYIKTEFYTYQLRGVPELFTMSDRKQHARRRRELSHLFSMSTITEYEPVIAKQVKNCMDFVADEGKTGRVSNLYDWWHYLSMDIICDLSFGSTFNMLQDGASNAYIKDLYGSLQIEPVRWHFGWLNKYAAWAPLKFVRDAEACSVRAMDRGAKLVRDYVENPEKKRRKDLLQKMLDARDEEGRPLSVDALNTEAAGFILAGSHTTSSSLTWIVWRIMKSPAILARLRKELDEVLTGLPRDEVPMHSKLEGLTYFNCVIKEGLRIDTAVPGSTPRYVPPEGTNINGHKLSGGSIVSAQAYSCHRDPAVFPNPDEFLPERWQDETPEMRKLYIPFGADGPRKCIGIWLAYMELRVILAALFHRFDLEFAHEVDDASMELDEFWLANPIGQHLDVVATERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9K6</accession>
    <name>A0A8H6C9K6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mannose-1-phosphate guanyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">GDP-mannose pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">GTP-mannose-1-phosphate guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Transcription factor SKN7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Translation initiation factor eIF2B subunit epsilon</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">eIF2B GDP-GTP exchange factor subunit epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">HO133_005228</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Transcription factor that is part of a SLN1-YPD1-SKN7 two-component regulatory system, which controls gene expression in response to changes in the osmolarity of the extracellular environment. Under low osmotic conditions, phosphorylated and activated by the phosphorelay intermediate protein YPD1. Also activated in response to oxidative stress, independent on the two-component regulatory system. Regulates heat shock genes in response to oxidative stress and genes involved in cell wall integrity in response to osmotic changes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the SKN7 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eIF-2B gamma/epsilon subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148837.1">
        <property type="nucleotide sequence ID" value="XM_037296139.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333634"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04197">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05787">
        <property type="entry name" value="LbH_eIF2B_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11558">
        <property type="entry name" value="W2_eIF2B_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000212">
        <property type="entry name" value="Stress response regulator/HFS transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000380">
        <property type="entry name" value="Transcription factor SKN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000066">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035543">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051956">
        <property type="entry name" value="eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056764">
        <property type="entry name" value="LbH_EIF2B3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044123">
        <property type="entry name" value="W2_eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25084">
        <property type="entry name" value="LbH_EIF2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 24" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="27">
        <location>
            <begin position="377"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="W2" evidence="28">
        <location>
            <begin position="1104"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="499"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="567"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1086"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1273"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="526"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="567"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="26">
        <location>
            <begin position="1275"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="24">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00057149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00061465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00070291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="KAF6219402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1292" mass="144633" checksum="A14702885737B8DC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA08</accession>
    <name>A0A8H6CA08_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005247</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148856.1">
        <property type="nucleotide sequence ID" value="XM_037296158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA08"/>
    <dbReference type="GeneID" id="59333653"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045072">
        <property type="entry name" value="MKRN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
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    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224:SF10">
        <property type="entry name" value="IP09428P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224">
        <property type="entry name" value="MAKORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="60"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
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        <location>
            <begin position="396"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
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            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
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        <location>
            <begin position="406"/>
            <end position="422"/>
        </location>
    </feature>
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            <end position="446"/>
        </location>
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            <begin position="488"/>
            <end position="550"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
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            <dbReference type="EMBL" id="KAF6219421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="615" mass="65130" checksum="0927CFEF847DAA34" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA52</accession>
    <name>A0A8H6CA52_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148909.1">
        <property type="nucleotide sequence ID" value="XM_037294862.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59332351"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="154" mass="17556" checksum="59427792D689F655" modified="2022-01-19" version="1">MAAPGPNPRPQQAWPSLPSLSDAETMVRQALENRHTWDTIILCTLVDDLFGRPIYSKEIASVISVRDGRTNVPFHTAIVVYMFDIAQLPFIEEVCPLTCRYKKWEEVKNKFMARNGQRDYDDMEEMSAWILYNETLTTANLIASNQLGSSSARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAA8</accession>
    <name>A0A8H6CAA8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Wbp11/ELF5/Saf1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149014.1">
        <property type="nucleotide sequence ID" value="XM_037294967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAA8"/>
    <dbReference type="GeneID" id="59332457"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019007">
        <property type="entry name" value="Wbp11/ELF5/Saf1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09429">
        <property type="entry name" value="Wbp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Wbp11/ELF5/Saf1 N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="329" mass="36479" checksum="933424299FA519C7" modified="2022-01-19" version="1">MAKDKDRAQNPAAQQRKLEKQKALKKGKAAVAAQRTERYASRNPRRLESTISDLHALKESQGGKLSARDQRQLEEAERDVARVKKAREVLGDKAPTFSSHRGGGEARGRGDGRRGGGGYGGLGKRNREEEESSGGETDESVRKIPWPRDTPPPVPRQRREDRPRHPTNANSEPLGAERRLQSREGAEAEVPDTTLPAKPAPKTTYESKPVVRDLRKEATARFVPNAVKRKIDATKGVGAKLLEEEEVEKLEKEGYGAGGRSFGDGVRGRSVVDAAPEIDGVDAEDERERRRLEEEEEQFRQEMEMVEAGGEEGHGPKRVMVEEVSEDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB43</accession>
    <name>A0A8H6CB43_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotide exchange factor SIL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003326</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with KAR2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149630.1">
        <property type="nucleotide sequence ID" value="XM_037294249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB43"/>
    <dbReference type="GeneID" id="59331737"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031884">
        <property type="entry name" value="Sil1_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF34">
        <property type="entry name" value="NUCLEOTIDE EXCHANGE FACTOR SIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16782">
        <property type="entry name" value="SIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="33"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6220195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="360" mass="39052" checksum="BE73F677A0CEF7DF" modified="2022-01-19" version="1">MEHFQRIHDDQSIPAGLHVRMNLATGLKEARLNIPEPPGTPHADLVIIDDLPPRPSIEEDGPVAEAPELQDQSHPDTEFERGSYYPASFDAEESSLFFSSIAAIHSTTALSTTDLPTLSALQDLAHSIHWGVALARDTGACQKFFSAIDPGSTASAEVRSAAALLLGTAIHSNPDALDAFLSHPYSSDVGMTPVVKVLAALRDPVQNDVTLKTRTVSLLSQLCQNTEQLRIFVQSEGLTTLFDLFEPEKMTLDEGKDRIRAKAANLIHDRILSSLGSANGFVSQSGSEISGLKDHHALVKGLEPWCNAFTKALRKYKFVAMSGENVSPAADAAYESIKEANQKLREGNVQSRICENESEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB89</accession>
    <name>A0A8H6CB89_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149524.1">
        <property type="nucleotide sequence ID" value="XM_037294143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB89"/>
    <dbReference type="GeneID" id="59331631"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="240" mass="27146" checksum="FD235636E9900E41" modified="2022-01-19" version="1">MAIQEIILPIDQGDTSTPKKLSSREGPISYESSFWADVRERRATPARTKAMLYVKIRHCKIEHGDKSENWYIGHVLHYGGGPAKIGHAAVRQRVVEAPEQVEEQGTQVWDNFRAGSLDVEEDMHEAEPWIGGVPAVNGLSWRREYEAEKHAKKTREVEGRLGQKSEESRNLGNSGTKMKKPARDVKDIRKEWKRSNEKAHVRDAAGHLGGWHGQYEHGDDEEDEEGSGSNRSDGLRLIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB93</accession>
    <name>A0A8H6CB93_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aromatic amino acid beta-eliminating lyase/threonine aldolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003387</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149690.1">
        <property type="nucleotide sequence ID" value="XM_037294309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB93"/>
    <dbReference type="GeneID" id="59331798"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000030">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041359">
        <property type="entry name" value="GntG_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="L-THREONINE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="6">
        <location>
            <begin position="129"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="44"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="467"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="486" mass="51772" checksum="385198D21E794586" modified="2022-01-19" version="1">MVTPTADAASRRKIYGVTSIDKYEYLPIVVQTANSLIFHEQLLQSTSSSSSKPEATMTESKQRPEAVTNGHKTANRILAPSTTDGPNAWSTPGSAAFDFRSTSSSQPIPSSHTLLPQAPNPTKLTTLPGDTVTSPTASMLLATQKCTLQDDVFQEDPTTNSLEAYVADMTGKEAGLFVISGTMGNQLSVRTALGGPPHSVLADARSHVYGWEAGGIASLSGALAMPITPVNEHHLTLSDIKKHAVTSTDTHFCPTRLISLENTLAGTILPLKDCQEISQWAHAQNPPIHMHLDGARLWEAVAAGAGGLEEYCACFDSVTMCFSKGLGAPIGSIIIASRPFVERARHIRKGLGGGLRQAGVVTASARIGVDDTFLGNKLTATHRRAREIADVWQKKGGKLTQATETNMVWLDLDAAGVSREGFVEAGVKEGVRFLGGRVVVHYQVSEEAVGRLGRVMDAVLSAPAQEPETKNGVREEARGVMAPEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBA8</accession>
    <name>A0A8H6CBA8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149554.1">
        <property type="nucleotide sequence ID" value="XM_037294173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBA8"/>
    <dbReference type="GeneID" id="59331661"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="442" mass="52576" checksum="A7D6EA49C75CB8FF" modified="2022-01-19" version="1">MKRVLRRYRRQKRPKTQGSPDMVVYKPRQVQKNRIVPQRTNLEDVPVEIQQTILHQMPDLPTLQALISASPSYLRAYQSQRHSILSNILLRDIHPDVLFDVLAIVDALKLPRNYDDYVPQMKVFIEQYKTARDSLHVALKQLEPSIKENVWGFHLSVIDVTKDFCDYALSTHPVTGHSLNHRTSLSPNEVRRIHRAFYRYELFTVLFRESDYYREEQTRRRRDRDPGRVRLAVQRDSIRSLDDQDRSFLFFALFRAWEVEEIACVRDYIIHRYDELHKQCKSEWQEMTGRESHYPAAPWNDSSEWVSEMYDDQSCHEHYLSLGLGFFCTLMKASHEDQMQMLRENIGLSDSTLSRALEENGPEWDNPMYDAWVTEAFLGFYRDSDRDAPNAAWAWSTGDKVDIRYYQSDKAGLRTWGYVMWDKERLDQWSILHENPHDYLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBW5</accession>
    <name>A0A8H6CBW5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_003003</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150005.1">
        <property type="nucleotide sequence ID" value="XM_037293927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBW5"/>
    <dbReference type="GeneID" id="59331415"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051735">
        <property type="entry name" value="CFEM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928">
        <property type="entry name" value="CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G14090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928:SF2">
        <property type="entry name" value="GPI ANCHORED CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G10580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6 15" id="KW-0349">Heme</keyword>
    <keyword evidence="10 15" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034092294" description="CFEM domain-containing protein" evidence="17">
        <location>
            <begin position="22"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="18">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="30"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="44"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6220570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="192" mass="19496" checksum="93DD673B61E905F3" modified="2022-01-19" version="1">MHSKLPYLAPLVLLLSSRSHAQNLSNLPSCAQSPALSAIGSTGCQANDFACLCKDNSFINAALAGAEKQCSQADLQKVLAFAQQICGSVGVSLSVSASVEGSSTPSPITNTRTPTPMPSPVTTSTQVLQSASPSRSALAAATTATSQASDTATSKVRGTATSGASGWDARFGLRGMMWSGAVVGWWLFGVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC68</accession>
    <name>A0A8H6CC68_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150189.1">
        <property type="nucleotide sequence ID" value="XM_037294111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC68"/>
    <dbReference type="GeneID" id="59331599"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053146">
        <property type="entry name" value="QDO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36440">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_8G07350)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36440:SF1">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_8G07350)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="56"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="177" mass="19522" checksum="AEB989999CE5D8C2" modified="2022-01-19" version="1">MATREDPPTTRLSTPHVPIHFVPAKAGEIIKLGSALTLRVMEDGSHTDNRLGAVELIIPPRTSGPPLHWHEMHDETFLVTKGTVRFHYPVMEGPGDGDGGFHADARVGDYVVVGTRAPHTFSNPFGEEARFFNTFTPAFYVNYFKLMAELLQGGEMTPEVNMQAMASYATIPVEKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCF2</accession>
    <name>A0A8H6CCF2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_002618</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150372.1">
        <property type="nucleotide sequence ID" value="XM_037293543.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331030"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18659">
        <property type="entry name" value="CD2_tandem"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000130">
        <property type="entry name" value="Chromodomain-helicase-DNA-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041150">
        <property type="entry name" value="Cdh1_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056302">
        <property type="entry name" value="CHD1-2/Hrp3_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025260">
        <property type="entry name" value="CHD1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF14">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18196">
        <property type="entry name" value="Cdh1_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13907">
        <property type="entry name" value="CHD1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23588">
        <property type="entry name" value="HTH_CHD1_Hrp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01176">
        <property type="entry name" value="DUF4208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="14">
        <location>
            <begin position="277"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="14">
        <location>
            <begin position="377"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="474"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="776"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1050"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1316"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1138"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1059"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1086"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1344"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1359"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1384"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1399"/>
            <end position="1410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6220937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1549" mass="175928" checksum="D4E363EC757ECE31" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE04</accession>
    <name>A0A8H6CE04_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001652</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151119.1">
        <property type="nucleotide sequence ID" value="XM_037292580.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330066"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF62">
        <property type="entry name" value="LIPASE ROG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAGKc" evidence="4">
        <location>
            <begin position="115"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="678"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="723"/>
            <end position="777"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="808"/>
            <end position="879"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="927"/>
            <end position="952"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1150"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1243"/>
            <end position="1401"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1467"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="768"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="808"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="825"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="859"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="934"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1187"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1252"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1279"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1347"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1373"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1391"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1469"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1522"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1542"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1556"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1644"/>
            <end position="1654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1654" mass="180649" checksum="20B9667E5A2FE57A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE76</accession>
    <name>A0A8H6CE76_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151328.1">
        <property type="nucleotide sequence ID" value="XM_037292789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE76"/>
    <dbReference type="GeneID" id="59330275"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF16">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="180"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="636" mass="72028" checksum="1BE664EC9DA08220" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEZ9</accession>
    <name>A0A8H6CEZ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable vacuolar protein sorting-associated protein 16 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001244</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Essential for vacuolar protein sorting. Required for vacuole biogenesis, stability and to maintain vacuole morphology.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151593.1">
        <property type="nucleotide sequence ID" value="XM_037292174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEZ9"/>
    <dbReference type="GeneID" id="59329660"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000635">
        <property type="entry name" value="Probable vacuolar protein sorting-associated protein 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps16 N-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="3">
        <location>
            <begin position="501"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="831" mass="93099" checksum="011EC45784CA8EAD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFT1</accession>
    <name>A0A8H6CFT1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000719</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152018.1">
        <property type="nucleotide sequence ID" value="XM_037291656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFT1"/>
    <dbReference type="GeneID" id="59329137"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032862">
        <property type="entry name" value="ALKBH6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46030">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46030:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="8">
        <location>
            <begin position="115"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6222672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="257" mass="28397" checksum="672FCE93A5C43DFB" modified="2022-01-19" version="1">MPQDASPVSMYDCSLPASLQEVLIPSLPASGYYVPNFITETEEAHLLQKINNAPLPTWKTLAHRRLQAHPSPLSPTNTLLAAPLPTWLRDPVVPRLLSLPVDLIDSIFHNSPHGTPNHCLINEYLPGQGIHAHEDGDAYYPVVATVSLGSHIILDIKAKKNNTMGEKSQDMPRWRVLQEPRSLLITTGELYTDCLHGISEVKIDEVLGESSIANWNLLGDRTPFAGGQAAREPRVSLTFRDVIRVKKLGKAFGILNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFW5</accession>
    <name>A0A8H6CFW5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152063.1">
        <property type="nucleotide sequence ID" value="XM_037291701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFW5"/>
    <dbReference type="GeneID" id="59329182"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="341" mass="38972" checksum="CEBE995156024D6A" modified="2022-01-19" version="1">MAELELLHHFTTETCYTLSDRSESHELWRVTVPQVAFQHDFLMRGILAISALHLGCLRPDRQTDWGHVAAKQQDAALSSFRKIMTNMDESNCDAFLALSSLIVVYGFESPKASHSLGMFDYKGQDSDEWLPLIRGVNSIIMSVWPWIKNGRLSGLLHDHIQEPPQTELPSVLSEQLTHLADMCDRASGGPEAVNAYKAALETLRVSFVRMNNRPPYECEVSIAFLWPVMIPQEYIMMLNEKRPEALVILAHYCVILHHLDDYWWMRGWAMHIIDNIHRELDENWLYFVQWPTNVISVDQKVLTDGTLQNPISGNRVSNNFNTAIDSSLNIPNEEAKAGNDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG64</accession>
    <name>A0A8H6CG64_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CBF1-interacting co-repressor CIR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001011</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152306.1">
        <property type="nucleotide sequence ID" value="XM_037291944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG64"/>
    <dbReference type="GeneID" id="59329429"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022209">
        <property type="entry name" value="CWC25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051376">
        <property type="entry name" value="CWC25_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10197">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12542">
        <property type="entry name" value="CWC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="9">
        <location>
            <begin position="11"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="152"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="252"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6222960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="347" mass="40166" checksum="1ADB228768956F5E" modified="2022-01-19" version="1">MGGGDLNLKKSWHPVLMSNQRRVWEEEKKALDERKRIEQMMKERQEERNIQEIQDMQEAAGGTKRAQRVEWMYSGPSAGQTGTTEEMEGYLLGKRRVDGLIKGTENKKLEKASTEDSFMALQNANTVRDTAAKIREDPMLAIKQREQAAYEALMNDPVSRRRLLKEAGHDVKPKEKERRRKHRHHHRNDRDDARYLSPKRRRTGEEDRRSRKSRSSRSPPRQQTNGHHLNHSNSFPSNRPPPRHNTYSPEAQPKEKPRDQAVDKSARLAAMQADANDLDAVRSNRLAAIEERERVEREAEQKARDQSGKYGGRGDFVNGLNRRAGEMDVAERIQRNRGGLERGLDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGS4</accession>
    <name>A0A8H6CGS4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000975</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152270.1">
        <property type="nucleotide sequence ID" value="XM_037291908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGS4"/>
    <dbReference type="GeneID" id="59329393"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07652">
        <property type="entry name" value="F-BAR_Rgd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04398">
        <property type="entry name" value="RhoGAP_fRGD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000063">
        <property type="entry name" value="Rho GTPase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000041">
        <property type="entry name" value="Rho GTPase activator (Rgd1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF136">
        <property type="entry name" value="RHO GTPASE ACTIVATOR (RGD1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="53"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="476"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="345"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="365"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="409"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="667" mass="73173" checksum="E8CC23EB802D082C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGW0</accession>
    <name>A0A8H6CGW0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine transaminase</fullName>
            <ecNumber evidence="4">2.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000134</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-(phosphooxy)-L-threonine + 2-oxoglutarate = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-serine + 2-oxoglutarate = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152509.1">
        <property type="nucleotide sequence ID" value="XM_037291074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGW0"/>
    <dbReference type="GeneID" id="59328553"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00197"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000006">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003764">
        <property type="entry name" value="PRK05355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247:SF1">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="12">
        <location>
            <begin position="9"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class V" evidence="12">
        <location>
            <begin position="158"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6223292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="431" mass="47291" checksum="670590F28287E0D9" modified="2022-01-19" version="1">MPARTDVAYFGAGPAPLPTSVLEKGAAAFVNYENSGLGLAEISHRSSTATRILDETKKALISLLNIPDNYEILFLHGGGSGEFSAVVFNMVAVWVEKRRRRAQKEFGDNDEAILLRVKEELNYSLRLDYLVTGSWSLKASQEAAQLLEPLGRNFVNVALDAREANGGKFGTIPPEDTWKLTPADANAGCGSAFVYYCDNETVDGVEFPSFPKSLQPQAGEDERLVVADMSSNFLSRQVDISRFALVFCGAQKNIGITDLTLAIVRKDLLTTMPSPSFLHKVGVWSPPTILNWSVISKNNSLYNTMPIFSIWIAGEVMRRLLASYGDSKVAGQEAVSNAKAETIYRVLDANPEVYQPVNHKSVRSRMNICFRVKDAATEEEFLEGAETRMLQGLKGHRSVGGIRASNYNAVPMANVQKLAEYLSDFARANSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGW7</accession>
    <name>A0A8H6CGW7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sacsin/Nov domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152567.1">
        <property type="nucleotide sequence ID" value="XM_037291132.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59328611"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022155">
        <property type="entry name" value="DUF3684"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058210">
        <property type="entry name" value="SACS/Nov_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047352">
        <property type="entry name" value="P_loop_sacsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47839">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G04830)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47839:SF1">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G04830)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12449">
        <property type="entry name" value="DUF3684"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25794">
        <property type="entry name" value="SACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sacsin/Nov" evidence="2">
        <location>
            <begin position="29"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1400"/>
            <end position="1435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1759" mass="195446" checksum="9F86D41F70F1CBD4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH74</accession>
    <name>A0A8H6CH74_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component SEC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000297</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the SEC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152671.1">
        <property type="nucleotide sequence ID" value="XM_037291236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH74"/>
    <dbReference type="GeneID" id="59328716"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.30:FF:000004">
        <property type="entry name" value="Exocyst complex component SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.670:FF:000004">
        <property type="entry name" value="Exocyst complex component SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.30">
        <property type="entry name" value="Exocyst complex subunit Sec15 C-terminal domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007225">
        <property type="entry name" value="EXOC6/Sec15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046361">
        <property type="entry name" value="EXOC6/Sec15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042045">
        <property type="entry name" value="EXOC6/Sec15_C_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048359">
        <property type="entry name" value="EXOC6_Sec15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702">
        <property type="entry name" value="SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20651">
        <property type="entry name" value="EXOC6_Sec15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04091">
        <property type="entry name" value="Sec15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025007">
        <property type="entry name" value="Sec15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC6/Sec15 N-terminal" evidence="7">
        <location>
            <begin position="51"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex subunit EXOC6/Sec15 C-terminal" evidence="6">
        <location>
            <begin position="401"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR025007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="814" mass="92848" checksum="C38516174BE3DFEE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHA8</accession>
    <name>A0A8H6CHA8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cohesin loading factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000245</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCC4/mau-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152620.1">
        <property type="nucleotide sequence ID" value="XM_037291185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHA8"/>
    <dbReference type="GeneID" id="59328664"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019440">
        <property type="entry name" value="MAU2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21394">
        <property type="entry name" value="MAU2 CHROMATID COHESION FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10345">
        <property type="entry name" value="Cohesin_load"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="772" mass="86352" checksum="07EEBC992BB4A4BF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIE1</accession>
    <name>A0A8H6CIE1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL32m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010628</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153114.1">
        <property type="nucleotide sequence ID" value="XM_037301480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIE1"/>
    <dbReference type="GeneID" id="59339018"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051991">
        <property type="entry name" value="Mitoribosomal_protein_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01031">
        <property type="entry name" value="rpmF_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL32M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="94" mass="10408" checksum="976770657A9BD537" modified="2022-01-19" version="1">MSTVTRFDVPSILAGLWESILRAVPKKKTSHRKKRQRFMAGKALKDVTALNKCSACGNVKRAHLLCPFCVNEIRDMWGEKGSEGSKETTATVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ22</accession>
    <name>A0A8H6CJ22_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Vesicular-fusion protein SEC18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_010970</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153453.1">
        <property type="nucleotide sequence ID" value="XM_037301819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ22"/>
    <dbReference type="GeneID" id="59339360"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000026">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000166">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000012">
        <property type="entry name" value="Vesicle-fusing ATPase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000187">
        <property type="entry name" value="Vesicular-fusion ATPase SEC18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039812">
        <property type="entry name" value="Vesicle-fus_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078:SF3">
        <property type="entry name" value="VESICLE-FUSING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078">
        <property type="entry name" value="VESICULAR-FUSION PROTEIN NSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="15">
        <location>
            <begin position="76"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="326"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="608"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6224393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="816" mass="90350" checksum="5C0715AF663071E5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKG4</accession>
    <name>A0A8H6CKG4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable acetate kinase</fullName>
            <ecNumber evidence="5">2.7.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Acetokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010390</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetate + ATP = acetyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:11352"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the acetokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.1" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154060.1">
        <property type="nucleotide sequence ID" value="XM_037301243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKG4"/>
    <dbReference type="GeneID" id="59338781"/>
    <dbReference type="UniPathway" id="UPA00340">
        <property type="reaction ID" value="UER00458"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008776">
        <property type="term" value="F:acetate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006083">
        <property type="term" value="P:acetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00020">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004372">
        <property type="entry name" value="Ac/propionate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00016">
        <property type="entry name" value="ackA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="ACETATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060:SF15">
        <property type="entry name" value="ACETATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000722">
        <property type="entry name" value="Acetate_prop_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01075">
        <property type="entry name" value="ACETATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="214"/>
            <end position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="5">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="5">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="412" mass="44551" checksum="8DC1246AB754D750" modified="2022-01-19" version="1">MAIILSINSGSSSVKVSIYKSAAQGHPPTQLADASISGLTAPPAQLTYERGSEEIKNQEVKGIRSNEDACEYILDHLVKDGGLSEIASRDDITHACHRVVHGGDYPAAHVIDEDTYHHLQDLTDLAPLHNASALAIVEACMKALPKAKNIAYFDSSFHSTMPKEIETYPIDPEIAKKNKLRKYGFHGISYAFITRSVAEFLKKPESETSIIALHLGSGASACAVKNGQSLDNSMGLTPLAGLPGATRSGDVDPSLIFHFTHDAGKPSPGSTKELHISTAEEILNKKSGWKALAGTTDFGTISSSSDPRCKLAFDILVDRILGFIGSYYVKLEGKVDALVFAGGIGEKAALLRKRILEKCRCLGFEVDDEKNDKAIEDVVQDIGKEARHRTLVCQTDEQFEMARQCAVGLQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKQ9</accession>
    <name>A0A8H6CKQ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nickel/cobalt efflux system</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NiCoT transporter (TC 2.A.52) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153763.1">
        <property type="nucleotide sequence ID" value="XM_037300946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKQ9"/>
    <dbReference type="GeneID" id="59338482"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015099">
        <property type="term" value="F:nickel cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004688">
        <property type="entry name" value="Ni/Co_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011541">
        <property type="entry name" value="Ni/Co_transpt_high_affinity"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31611">
        <property type="entry name" value="HIGH-AFFINITY NICKEL TRANSPORT PROTEIN NIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31611:SF0">
        <property type="entry name" value="HIGH-AFFINITY NICKEL TRANSPORT PROTEIN NIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03824">
        <property type="entry name" value="NicO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="433" mass="46549" checksum="A95F116BA8BDE0EA" modified="2022-01-19" version="1">MGFPALKNGINRRAIACHGRIPGLRTLPFPAVGIIVGLVFANAIVWVAIGILLHFHIALVSTAVLSYTLGLRHALDADHISAIDLMTRRLIASGQRPVTVGTFFSLGHSTIVIVTSVVVAATASAISSRFGAFSKIGGIIGTSVSAGFLIILGITNIYILHKLVQLMRKLIASAPGDEQSFEIKGAGCLFYLFKRMFKLIDRPWKMYPLGLLFGLGFDTSSEVALLGIASIQGAKGTSIWLILIFPVLFTVGMCLLDTIDGALMMALYTSTALAKDQIAILYYSIVLTVITVMVAMIIGFVQLLTLVLNVAQPSGKFWGGVAVAGDHYDVIGGAICGSFVVFGGLSALLYKPWRRRIDRRRERHPQHQQLQNDKGLGTLPELNGVLLDFDVSQRDGETPTNGNDNSDNPNVFEAEILPTFTSCHGAPSRSAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL80</accession>
    <name>A0A8H6CL80_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154088.1">
        <property type="nucleotide sequence ID" value="XM_037301271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL80"/>
    <dbReference type="GeneID" id="59338809"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="156" mass="17845" checksum="C3DA940A89362D66" modified="2022-01-19" version="1">MVPEPKTNNSKDGAGKQYSRDSIRCLVRVNEIDAQHDFWDDCDRKISDISPKIMQIKRADVMDILARVPRLRWMSKSRKEKAREGEGEGDDGKEGRWDRVSRRERATHGNAPTTFESQRPFDSQGNQGLACEACVVSGSCIWPKSKWGYAEEERVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLB0</accession>
    <name>A0A8H6CLB0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154337.1">
        <property type="nucleotide sequence ID" value="XM_037300489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLB0"/>
    <dbReference type="GeneID" id="59338023"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="325" mass="35445" checksum="1EA15C91048363E8" modified="2022-01-19" version="1">MFQQQAQEYLANSSWELGSAITEYFNRAEGIDEEAQAPDASNPPSSAPQPMPPSSRGFLSSPATQAPKKKFATLGDLSGGRGPGHAGHGHGGGNDDDDDARGQGCRFFFCKVYLLLNSLQIGVAEPDEPFADKGDRYYVERGNMNLGMGSPYPFPMMALAAPVAPYTETPVHELVLSNSKPAEFLAIRETEDFKKLAFIYRSNQDAMDEDYIDKQAHVIQPTDICHETCNAESAIAVYYVGQGSGHRSGEVNIPMLVSRDGSITHEWTILSSEWQHISSSDVVGDSGAWVIRKHGNRLMGQVLAYSAGQVLFTPIDVIFADLKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLM4</accession>
    <name>A0A8H6CLM4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dolichol-phosphate mannosyltransferase subunit 1</fullName>
            <ecNumber evidence="4">2.4.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009781</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a di-trans,poly-cis-dolichyl phosphate + GDP-alpha-D-mannose = a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + GDP</text>
            <dbReference type="Rhea" id="RHEA:21184"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154488.1">
        <property type="nucleotide sequence ID" value="XM_037300640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLM4"/>
    <dbReference type="GeneID" id="59338176"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="5">
        <location>
            <begin position="8"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="5">
        <location>
            <begin position="62"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="201" mass="22787" checksum="639BFC4688DC5CAF" modified="2022-01-19" version="1">MSSKEKYTVILPTYNERRNLPIITWLLNRTFTENKLDWEIIIVDDGSPDGTLQANLDWVLHFSHHPKFIAPMIEKQKTGDYDIVTGTRYSGDGGVYGWDLKRKLVSRGANLFADTVLRPGVSDLTGSFRLYKRAVLESAIAQTESKGYTFQMELMVRAKGMGYRVAEVPISFVDRVYGESKLGGDEIVEYAKGVLNLWLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLS1</accession>
    <name>A0A8H6CLS1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear protein Qri2/Nse4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009762</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154469.1">
        <property type="nucleotide sequence ID" value="XM_037300621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLS1"/>
    <dbReference type="GeneID" id="59338157"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025638">
        <property type="entry name" value="DUF4336"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33835:SF1">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33835">
        <property type="entry name" value="YALI0C07656P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14234">
        <property type="entry name" value="DUF4336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="30360" checksum="F4AA694DACDD1646" modified="2022-01-19" version="1">MTSKLIPSDPSKVMVIRQVTPNITTCSAPFLRFGRLKVGGRGTIVRLQSGALAVFSPTALTPEVRSTVESMGNKVGYIAAGDYEHHIFITEWAKAYPSAKLLGVEGLAEKREKDQATAGSKFQHVWTQKNKANMKVDPEFDEEFDYEYVGSHANKELVLVHKRDRTLIEADMMFNLPAKEQFSRTGEDPTSGTLTRLFIGLQNTMGPAKWQKRFLWYVASAGDRQGFNQSARKIASWDFDRIIPCHGDVIESGGKSIFKKVFDWHLNASVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM29</accession>
    <name>A0A8H6CM29_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154340.1">
        <property type="nucleotide sequence ID" value="XM_037300492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM29"/>
    <dbReference type="GeneID" id="59338026"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="318" mass="35406" checksum="5AA8BBD14D00C07D" modified="2022-01-19" version="1">MSAPKDRLDKVFAQCLQHHPEGHATYEQISARDLKPGACGYIDENGAWNKIAQMTDEAAMNMLGWTCPKDLATVEKAGKEKWPQAKTSKNLSWSSTDVDAGADVPGMQAGGRIKMEYQKAEGNGAIMMADGEVKHRRAKPLSTAVDWLAQNASSILEKFKDAQTNGIWVVTSTHVAERRVLAVLSSHQTTFSWTVEVKAADVGSIAPSSSWVRERQDGSWIEQEDKDGVVLFFCGIQWDPRRIFRKLRRQREDQKYLGADEDKGLDAIEVELDSSGIEGEARFTRFKLVPKLVGTVEDVHLKADYDSEDDSEDEEDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMZ7</accession>
    <name>A0A8H6CMZ7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154634.1">
        <property type="nucleotide sequence ID" value="XM_037299808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMZ7"/>
    <dbReference type="GeneID" id="59337341"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="401" mass="45091" checksum="8BF4D63DC1414AD7" modified="2022-01-19" version="1">MTRSKRTLAETDANAEVAPASKRTSTGKARSKENDAIQPTADENEPAVSSTAKSPASKQQTPSKNKEAKVPGDADGKTWMCLCHVRGKRCGWDKSCMCGRYADANPAETWIVTKKGFELHKDWGLEQYQRDQDNFRMHIFSDWSGYGTCEVMENMVRTIGIYYNDQQVLNSGMVSDSAVKLDRTYSAHASRIDDSEGNAKRVMLFGTALLTTIDILIKNSLFKSENSDIRNIALILGHFLNFVHDMKEMCVANEDGWKATVLERADEHEIKPYMISINHIISEIREGKDDATSDEDGTPRPEIGTRREGLENAAKSYESKPWVGVITLESLKAGVERSWTHWDWATELRAYSKAQAVKVGPIGPNYGKRIGGKFYDLTIKGSQRRMKQYRIGPEESDEDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN11</accession>
    <name>A0A8H6CN11_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Senescence domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155055.1">
        <property type="nucleotide sequence ID" value="XM_037300229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN11"/>
    <dbReference type="GeneID" id="59337763"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009686">
        <property type="entry name" value="Senescence/spartin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045036">
        <property type="entry name" value="Spartin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068:SF43">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06911">
        <property type="entry name" value="Senescence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Senescence" evidence="2">
        <location>
            <begin position="247"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="501" mass="52484" checksum="C0A1A4987CE7820B" modified="2022-01-19" version="1">MSAGHNEPKLLYSVGNISAYHIQDGQESPLSHSGPQTLSLLMVPTSSPFADLSSANPSSAAPEEDFYLHLHLPPELDLSLPATTQIYHQPPRSYLIPRWDLGPESGAFTRIEFPKIGTGPDRVTQEDVDTFETILAQCTAFLERASFTSAGEKKYNPADFAPGEGYVGSGLGGTEKGHIVLVDEEDGSVVGELGEGFHVVEGKDVHAGSKRPVEIQLPQEGEGNQINITNASEEYLKMAKHPAYKDSTLVQNAAVASRLIVTGSGWLANQMTTGADNFTKKTKPNPKPMTFTPTTQARVRKLNSVTQGAVGLSAKTVGQVTRHAQNLGATLTRRSEQQKKNHKADREGYKPGLLNKSMMAFSTIADGVDYASRNLLASGSSAATQVVGHRYGENARNVAGSIAGGVRNVGLVYVDAAGVSRRAVIKSVAKGMVVGRAPGGGDLVVGGGDGGAIPPEAMPADKPGTVGNQSMGAGSGTQVGFGNKTAPITEKSSSLGEPLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN83</accession>
    <name>A0A8H6CN83_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155111.1">
        <property type="nucleotide sequence ID" value="XM_037300285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN83"/>
    <dbReference type="GeneID" id="59337819"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="358" mass="39489" checksum="6D85DB882FD28026" modified="2022-01-19" version="1">MPPLSVLISDGDPSDAFHDQFSTIIRLESRLQGDYSEDVTGPAHQRWVVKHLTEVAQAQGFPMQLPTQATAFNVHAGEFITPPPAQTTIFGHSGYTTPWHENFNLADQGVFDSSGPYYPAAQNIAFHRLSRPPSPYHPFDPAERAAYRAIVRAQLLQSSGQRAESQPHASSECFDHTSDAFIRSSDNLPRFEPPTTPPNNFSGVRSPFTPPNQSPRLRGQTAPPESPESLSDLVANTTIRASTEPIAESRMGSLTAPDCYKSIEVYAIDVQTGDYIQSVKNPGEVMDHYPCEDLDHEPIGENEARPVSPNGGLRIRFKGADGSTRTKVFKGLDKVRVLRQVAKDHDDGEKEVKKEESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNB1</accession>
    <name>A0A8H6CNB1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Molybdopterin synthase sulfur carrier subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Common component for nitrate reductase and xanthine dehydrogenase protein G</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Molybdenum cofactor synthesis protein 2 small subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Molybdenum cofactor synthesis protein 2A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Sulfur carrier protein MOCS2A</fullName>
            <shortName evidence="5">MOCS2A</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cnxG</name>
        <name evidence="6" type="ORF">HO133_009227</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by UBA4. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterotetramer; composed of 2 small (MOCS2A) and 2 large (MOCS2B) subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of UBA4, then thiocarboxylated (-COSH) via the rhodanese domain of UBA4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MoaD family. MOCS2A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNB1"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990133">
        <property type="term" value="C:molybdopterin adenylyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990140">
        <property type="term" value="C:molybdopterin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030366">
        <property type="term" value="F:molybdopterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00754">
        <property type="entry name" value="Ubl_MoaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03051">
        <property type="entry name" value="MOCS2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044672">
        <property type="entry name" value="MOCS2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028887">
        <property type="entry name" value="MOCS2A_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359:SF1">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="1-thioglycine; alternate" evidence="5">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="modified residue" description="Glycyl adenylate; alternate" evidence="5">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="96" mass="10281" checksum="594BA2A7085DD42D" modified="2022-01-19" version="1">MSGKPPSEHFRLLYFASAASFTRKSSDTFPAPLPISDLFSVLEKKYSGITKAVLDSCAVTVNLEYVDVEEESGKEKAGVVIKEGDEVAIIPPVSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNI3</accession>
    <name>A0A8H6CNI3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009360</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingosine N-acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155047.1">
        <property type="nucleotide sequence ID" value="XM_037300221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNI3"/>
    <dbReference type="GeneID" id="59337755"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF0">
        <property type="entry name" value="LD18904P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005225">
        <property type="entry name" value="LAG1_LAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="9">
        <location>
            <begin position="101"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="340"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6226494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="420" mass="48374" checksum="F511CC556ECA0D9E" modified="2022-01-19" version="1">MAVQKGQLFANWTTRKQIVVSWSLILSVTIIHSLVPLVRSYTRLFYELPYPQANGQYIQGSDDALFVLGWLVLMTAIRATIIECVYEFVTRLRLMSRKASMRFAEQAFLLTYDFTSFSMGMNILVNSSYWLNFEELWSTWPSREISGELKWYYLVQLAFWLQQLLAINLEKRRKDFGQMFLHHCVTSFLMYVAYAYRWTNVGNVILCIMDVVDFLLPLAKMLKYLHFEAACNTMFGIFLTTWLVARHGIYVKLCWSIYRDVPRVMPFACYSGETMELETDPSKLSRWRYFDPFFDQKGTICLDRNVKSIFLGLLLMLQVLSIVWFGMIIKVALSMLNGEGANDTRSGDEDEGGEDEEEEEEEEECEARSNGKRTQSANAKAKASGSEAPSRQTGLFQRGGIRVPGSRDRKELIGRVGCNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPC5</accession>
    <name>A0A8H6CPC5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Epoxide hydrolase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008415</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155282.1">
        <property type="nucleotide sequence ID" value="XM_037299281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPC5"/>
    <dbReference type="GeneID" id="59336811"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF35">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="405"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="413"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="574" mass="65233" checksum="FAE1F58887C97E19" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPF3</accession>
    <name>A0A8H6CPF3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mannose-P-dolichol utilization defect 1 protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_008475</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the MPDU1 (TC 2.A.43.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155342.1">
        <property type="nucleotide sequence ID" value="XM_037299341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPF3"/>
    <dbReference type="GeneID" id="59336871"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000006">
        <property type="entry name" value="mannose-P-dolichol utilization defect 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016817">
        <property type="entry name" value="MannP-dilichol_defect-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 LEC35 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226:SF2">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023381">
        <property type="entry name" value="MannP-dilichol_defect-1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR023381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6227034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="285" mass="30229" checksum="3589DD182DEFFDAB" modified="2022-01-19" version="1">MDALRTTIQPLTHSLPAPIRDFGVSLIGPDCYQNLILDIDLTSTQCLKLAVSKALGIATVAGSSVVKVPQIIKLLSSQSGAGISFLSYALETSALLTTLAYSARNGFPFNTYGETAMIAAQNVVISLLVLRYTGQTVLAAVFVAALASAGYSLFNEGVIDMQTLTYAQMGAGLLGVASKLPQVWTIYSEGGTGQLSAFMVFNYLFGSLTRIYTTLQEVDDRLILYGFVGGFVLNLVLAAQMVYYWNSPATASHAGEIGQKPADIVKGSSTRASTKGKGPTTRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ34</accession>
    <name>A0A8H6CQ34_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155569.1">
        <property type="nucleotide sequence ID" value="XM_037299568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ34"/>
    <dbReference type="GeneID" id="59337099"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="283" mass="30372" checksum="3C4EDB889E10A801" modified="2022-01-19" version="1">MPQDPSIKYHLEARVPEEDGAAGSEIGNDSGGGRTPGQEHGDGMTPQIPPAQYQPVENYGCHSLTEYAWMPEEIDGVVKSPGCAKTGKMVNDWPMPNPDGKWYDEWATDFDDSSDCNLYLAWAQGDNYWIAFARDASNHPMGGIAPQNRSALNPPGAFSLGTGGWIMTVTEATSDFSVISPPDNSGLSFVWAHSGGGDGEVAWASNYAFDDSNDGNQGGCKYSVDPTKPQKSQGGMLFASAHLETLQESGKSQIGSLGTHKISLRYAEGQTDLYEVLYASAEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ95</accession>
    <name>A0A8H6CQ95_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008795</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155659.1">
        <property type="nucleotide sequence ID" value="XM_037299658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ95"/>
    <dbReference type="GeneID" id="59337190"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057720">
        <property type="entry name" value="RRM_YTH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF3">
        <property type="entry name" value="YTH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25701">
        <property type="entry name" value="RRM_YTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="327"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="YTH" evidence="4">
        <location>
            <begin position="460"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6227351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="694" mass="75245" checksum="A34BD1374F0C83A0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ97</accession>
    <name>A0A8H6CQ97_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007608</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155814.1">
        <property type="nucleotide sequence ID" value="XM_037298478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ97"/>
    <dbReference type="GeneID" id="59336005"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057683">
        <property type="entry name" value="DUF7923"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057654">
        <property type="entry name" value="Znf-CCCH_tandem"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37543">
        <property type="entry name" value="CCCH ZINC FINGER DNA BINDING PROTEIN (AFU_ORTHOLOGUE AFUA_5G12760)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37543:SF1">
        <property type="entry name" value="CCCH ZINC FINGER DNA BINDING PROTEIN (AFU_ORTHOLOGUE AFUA_5G12760)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25540">
        <property type="entry name" value="DUF7923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25543">
        <property type="entry name" value="zf-CCCH_tandem"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7923" evidence="2">
        <location>
            <begin position="242"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Tandem CCCH zinc finger" evidence="3">
        <location>
            <begin position="571"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="71614" checksum="625C42F2FBB96A14" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQU5</accession>
    <name>A0A8H6CQU5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155869.1">
        <property type="nucleotide sequence ID" value="XM_037298533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQU5"/>
    <dbReference type="GeneID" id="59336060"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039323">
        <property type="entry name" value="ANKRD_45/46/60"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22677">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22677:SF4">
        <property type="entry name" value="USHER SYNDROME TYPE-1G PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="286"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="38299" checksum="5DBDFB11D5C4C3F5" modified="2022-01-19" version="1">MTDHFSVASGAVGVVSLGLTVCQGLLQYYSSWRHAQKDVDAMCASLKGLTKTFRLVKITIKDRKFNQPVVDRVTASIDLCETGVDELDAKLLKVKMTPVKENGDQIQGQFRRALYPFRESTPLKLREMISDLRDKLSLAISTLQLLHVYLKNMTSNQDAEDSLSLFNGYRLCISETNKKTLLTDGKKQPGISYYVTPVSRTGWEEKRERCVGFYQGDPSWRDIGARMKKNILLAYAAQNWGNHARKAPSHNTILDELVLEFLGRRANLVFSTGVIRHFLLGVAAPTDGTALNVAAFFGLEKTVLTLLREGYNVNAEDSCGWTALHVAAGTEHENIVQLLLEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRN5</accession>
    <name>A0A8H6CRN5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156349.1">
        <property type="nucleotide sequence ID" value="XM_037299013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRN5"/>
    <dbReference type="GeneID" id="59336542"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="1">
        <location>
            <begin position="71"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="265" mass="29663" checksum="1A6CAE31A583DA1D" modified="2022-01-19" version="1">MRNPDGVLDARANASAMRAGKIRGYHCDHAFDRVVSSAPAWHLVDVAEAEVVYAGYNVIARTHFSNSFPKNRGWGPVTLVKCCKQMYLEAIDLLYSLQIFSFASLTPPPFFMQSVLPHRLAQIRSIQLCYNQITMSSPCPSGGNDRRAALHAHRMQKCAACNLLTWSKLIKQTLKGLRTIEAFIYLGSTFTMPTPDAPWITRLLEMQCGSNGLRELKINVLPGPWMNVTAHPDGFLANAARLDLLLQSMIKKGAEKHRHRKQDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRS2</accession>
    <name>A0A8H6CRS2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156080.1">
        <property type="nucleotide sequence ID" value="XM_037298744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRS2"/>
    <dbReference type="GeneID" id="59336273"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40788:SF2">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40788">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="734"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="882" mass="100128" checksum="D651CF7A6DFCC00A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSY6</accession>
    <name>A0A8H6CSY6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">General transcription and DNA repair factor IIH subunit TFB4</fullName>
            <shortName evidence="14">TFIIH subunit TFB4</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">RNA polymerase II transcription factor B subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_007029</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TFB4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156559.1">
        <property type="nucleotide sequence ID" value="XM_037297924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSY6"/>
    <dbReference type="GeneID" id="59335429"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000084">
        <property type="entry name" value="Transcription factor TFIIH subunit Tfb4, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004600">
        <property type="entry name" value="TFIIH_Tfb4/GTF2H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03850">
        <property type="entry name" value="Tfb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="82"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6228917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="362" mass="38922" checksum="9F95D7BE014CCA1A" modified="2022-01-19" version="1">MNAVDGSDHYNKTSDEPPPSLLTIILDTNAHAWALLAPTLPLSAAIANLLVFINAHLAFNNSNKVAIIASHTQRAEFLYPIPSEDDSRQANGSTHNGAQNGLDGDMNMTDGNEANYAATADDANHYRPFRHIQTTLLSAMSKLYKTTSPESLTPTTSIAGALTLALTYINKQSIAFSTTTSSKTNADPLLAPSDPNSIQPATLNPRILILSVSGDLAHQYIPIMNTIFACQRLTIPIDICKIAGDSVFLQQASDATHGTYIALTHPRGLLQYLMLGFLPDQSSRKYLVPPTAVGVDFRAACFCHRKVVDVGFVCSICLSIFCSPPEGAVCLTCGTYLQLGEYGGKPAVVAKRKKKRKKGADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7K4</accession>
    <name>A0A8H6F7K4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147600.1">
        <property type="nucleotide sequence ID" value="XM_037297026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7K4"/>
    <dbReference type="GeneID" id="59334528"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053175">
        <property type="entry name" value="DHMBA_Reg_Transcription_Factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38791:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR DBAG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38791">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="509" mass="57414" checksum="5FCA11D5F6AF2549" modified="2022-01-19" version="1">MVFRGKPSKACHRCRERRLKCDLVKPRCTQCLRAGRKCPGYRPQSNDRFLDQTAEVVFKARLAEDARTEQRQSDQAMTKELPRSLSLPIEARATSFFLSNFIQGSNFEYLPSLYAPYSVREEHLSASIEAVGLASLSNELRSLQASKQGRKRYIHAIQATNTALRSPVRATEDSTLLSVLLLSLYEAITCTTQLTLCLWESHIKGAMALIRLRGRQQMNTQLGLQLLSQTTFSVAISAHRNMAAVPTEITSLVTHALQYASENDPSWSLKLISFRAADLRAAIRSGSLSDPDVIIAAAMQLDHDFVLWSSTLPPSWQFKTVLAEHADGTLVYEGYYHLYSTHGIARSMNAWRMLRMQLKLTIREQILRQHTSPLRSQDYTTLMRHAESIITQLASEICATIPQYVELPARVPISVSKTQPPHSTISDIPLPKTESSTSGFTHAARSYGVIWPLMSVATCPVCHSSRRAWIINRLQYIGRQMNNPQALLAVKILERNWDVDIRMHLCHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F801</accession>
    <name>A0A8H6F801_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytosine-specific methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005998</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="EC" id="2.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148082.1">
        <property type="nucleotide sequence ID" value="XM_037296901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F801"/>
    <dbReference type="GeneID" id="59334403"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018117">
        <property type="entry name" value="C5_DNA_meth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057215">
        <property type="entry name" value="DUF7893"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00675">
        <property type="entry name" value="dcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF54">
        <property type="entry name" value="DNA METHYLTRANSFERASE DIM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25423">
        <property type="entry name" value="DUF7893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00094">
        <property type="entry name" value="C5_MTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BAH" evidence="11">
        <location>
            <begin position="442"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="BAH" evidence="11">
        <location>
            <begin position="575"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="693"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="693"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6218647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1270" mass="143087" checksum="6BAFA5574AA60A8A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F890</accession>
    <name>A0A8H6F890_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="7">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005460</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148352.1">
        <property type="nucleotide sequence ID" value="XM_037296370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F890"/>
    <dbReference type="GeneID" id="59333866"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF25">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase N-terminal" evidence="10">
        <location>
            <begin position="48"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="9">
        <location>
            <begin position="265"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="353"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="444" mass="50323" checksum="1315AD4B7F93278B" modified="2022-01-19" version="1">MPRSRSWALEKAAWSTRPHLFRRLIASATAPWRPAAVLDEWVQKEARPISLRQLTFFGRTLTENRLLSSANYVRMELPTRLAHRLRDMQTLPFVVVTNPHMSHVYDLYYKAFETLRRIREIKSVEDNEQYCKVVSETLQEHLTVIPRLAMGVLECQDLMRPEEMDKFMNTLLRSRISRRVIAEQHLALTETFNSPWHFPNAKSQVGANSEFVGEVFLRCNAREVVEACWNQASTLAKEAYGSSCILPDITIEGHLDATFPYILSHLQYVIGELLRNSIQAVVEGRSALNDSPPPIEVLICEAPQHVIIRISDQGGGIPNEVMPELWSFSKGPRSQTHLRNLSQVPKMAATMQELKPPNCSSDNSSTSNRDKKRSYDGSLGSLTSRPPNLRLGMGLPMSRVYAEYWAGLLEMHSLEGYGVDAFLQISKLGNKNEQLSTRASMDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8R7</accession>
    <name>A0A8H6F8R7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_003939</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148907.1">
        <property type="nucleotide sequence ID" value="XM_037294860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8R7"/>
    <dbReference type="GeneID" id="59332349"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000014">
        <property type="entry name" value="Aquaporin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF199">
        <property type="entry name" value="MIP17260P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="266"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="266"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="31867" checksum="A6F6297CAEAA5CCA" modified="2022-01-19" version="1">MNTGAPITAPVDGPVRRLLRTLPPTTRGHTVAVIGEFVGTISFLFFAFAGTQVANISSNTNTGTTVITQVAEKNPSELLYISLSFGFSLAVNAWVFFRISGGLFNPAVTVGMVLIGAISLVRGILLFVIQIVAGIVAAYIVQALFQGKLAVSTTLGGGTTIAQGVIIEMILTAQLVFTIFMLAAEKHQGNFIAPVGIGLSLFIAELVGVFWTGGSLNPARSFGPAVASHTFYTTQWIYWVGPICGSLFAVLLYKLIKALEYESANRDPELGPSAEKMKELLNGNGNVNGNGNGNGRVLSPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8U2</accession>
    <name>A0A8H6F8U2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_005532</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX48/FAL1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148423.1">
        <property type="nucleotide sequence ID" value="XM_037296441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8U2"/>
    <dbReference type="GeneID" id="59333938"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18045">
        <property type="entry name" value="DEADc_EIF4AIII_DDX48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000498">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="97"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="278"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6218988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="440" mass="50032" checksum="EE19992CB6DE3AB3" modified="2022-01-19" version="1">MPAAEPRLNISDTFCPSSLPHQHISYSKSTVTFQQHSRYPAVMAGIDRNMEEKMEFATSKEVTVAPTFENMQLKENLLRGIYAYGYESPSAVQSRAIVQICKGRDTIAQAQSGTGKTATFSISCLQVIDTALRETQALVLSPTRELATQIQSVVMALGDYMNVQCHACIGGTNVGEDIRKLDYGQHIVSGTPGRVADMIRRRNLRTRNIKILVLDEADELLNRGFREQIYDVYRYLPPATQVVVVSATLPYDVLDMTTKFMTDPVRILVKRDELTLEGLKQYFIAVEKEEWKFDTLCDLYDTLTITQAVIFCNTRRKVDWLTDKMREANFTVSSMHGEMPQKERDSIMQDFRQGNSRVLISTDVWARGIDVQQVSLVINYDLPSNRENYIHRIGRSGRFGRKGVAINFVTSEDVRILRDIELYYSTQIDEMPMNVADLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9A6</accession>
    <name>A0A8H6F9A6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005174</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148784.1">
        <property type="nucleotide sequence ID" value="XM_037296086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9A6"/>
    <dbReference type="GeneID" id="59333580"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000059">
        <property type="entry name" value="Serine palmitoyl CoA transferase subunit LcbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF2">
        <property type="entry name" value="SERINE PALMITOYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="89"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="475" mass="52616" checksum="4BC7DD5DD03E5470" modified="2022-01-19" version="1">MDLQDVQSWLNDILMEASIHFHKVPGSAIFLRYVKSSYQNDPEVDELIDEWTPEPLVAPLTLFEEAENEKRPVIVGPTGPKSKLSNGRTVTNLASYNFYNLISNETLKEKAIQVLRTYGVGPCSPPGFYGTQDVHIKTEADIAAHLGTPACIVYAQAFSTISSVISAFSKRGDIIVADRAVNYAIRKGIQISRSTIRWYEHNDMHDLQRVLENVVTEQAKKPLTRRFIITEGLFENIGDMIDLPKIIELGRQYKFRLILDETWSFGVLGRTGRGITEHQNVDAAEVDMIVGSLAGPLCAGGGFCAGSDAIVEHQRISASAYTFSAALPAMLATTASEVLSMLQTNPEMLTTVRENIKTMWAQLDPRSDWVHCTSSAENPIIVLTLKADLIASKKLRIADQEQLLQEVVDETLANGVLITRLKSMPRAINATTKDIGWELQPALKVCVTSGLSKKEIERAGITIRHAVTKIVNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9B0</accession>
    <name>A0A8H6F9B0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149191.1">
        <property type="nucleotide sequence ID" value="XM_037294503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9B0"/>
    <dbReference type="GeneID" id="59331992"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="517" mass="54045" checksum="F2E26F9E080B2573" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9P7</accession>
    <name>A0A8H6F9P7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003517</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149820.1">
        <property type="nucleotide sequence ID" value="XM_037294439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9P7"/>
    <dbReference type="GeneID" id="59331928"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="131"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="266" mass="29522" checksum="D324CFB4C08BAC83" modified="2022-01-19" version="1">MAILAPSPTPDQLQAALQSLLDGPAGIPPTGVIPNFDNPPNKDIYLYVTATLTIWFATCAVIIRIYTKHFLLRSMGYEDYTSVIAWISFIGFAVPSFLCSKSGAGTHMWDIRLKSFFRVLYWVNITAITYAITMSLERKIMTMGVFATGFFACVTSILRTYYTWQTVETLDTSWDLLFMGLWTWAELSTGIIVGCLPALPKFFQHVGPKIYRTIPGTGPRRNSGAAIDTPQVIALAKFKTPFAKYGVGPSVSDSSNDPYSLRARLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9X1</accession>
    <name>A0A8H6F9X1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003879</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149489.1">
        <property type="nucleotide sequence ID" value="XM_037294801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9X1"/>
    <dbReference type="GeneID" id="59332290"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034748793" evidence="3">
        <location>
            <begin position="16"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="253" mass="27840" checksum="4081B322DC462CF3" modified="2022-01-19" version="1">MAIVFLVIAAGTSFGSPTTNHIRPPKKPIPPHNNVPAQSPDVPNNNLHALITTRALPDDSNRHARRNKKYWLTSSNHPSPALLQRHNHITDHLRLHSAASSSSSSSPPLTKRSLASDLTVMGFRLIWTHADVIVSSSLAYYRTTEYYRNMTVLAGGEFGFGPTVQNYVITYGLLRLTFDVVADAAEAIAAELAWEFPNGFGQFVQEFAQVMLWLTAGVVIGTYTVLAWTVTTAVWITMVIVENVDLRNMVSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA08</accession>
    <name>A0A8H6FA08_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149896.1">
        <property type="nucleotide sequence ID" value="XM_037293818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA08"/>
    <dbReference type="GeneID" id="59331305"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="308" mass="34829" checksum="DBAC545426B7C889" modified="2022-01-19" version="1">MVRPQDRSKAYGQRAHGHEPDRRSTGGGPHQGSRSGSDMTRRKIREIDEEGMDDFVNDFEEMGMDAGRNSTSGGPQGRRGTGRRFFYDMDDFDEEDIDPRRPSARGGPQGRRDTRPRHLDNMDGFEGEDIYPRRASTRGGPQGRRGVGPRYMDETSTDMVPYVAGSGYGSKAQTANPSTAKSKPSLGELEAELDRVEMEQERLGDKIMQIKMEIYRIEPESERFNDSDRSFLHLPSKGNRNHEERGSRHGRRGPAPLRVGNLDRGAARHREHDQRSGKDVRLYKILFDGLADLSSSRRSPVRRFAPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAM5</accession>
    <name>A0A8H6FAM5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002454</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150209.1">
        <property type="nucleotide sequence ID" value="XM_037293380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAM5"/>
    <dbReference type="GeneID" id="59330867"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="169" mass="19707" checksum="E5300DC7BC3DC90B" modified="2022-01-19" version="1">MPSTGFLRLLPELRNTVYQHIYIRARRYVSGRDWSSATYGNGLFFTCRQIYQETIHQYHSFDFVFLTGSTTDYSWLDKIGSEVRNKLLNAAVDTESFGVEPRMFFYMLSRCEQLSRKIGIRLSRLISMWQNGAFESMHGFHWARAIASPPVRVEVRVGSTWVNGILSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC50</accession>
    <name>A0A8H6FC50_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_000743</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152042.1">
        <property type="nucleotide sequence ID" value="XM_037291680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC50"/>
    <dbReference type="GeneID" id="59329161"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14004">
        <property type="entry name" value="STKc_PASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000320">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000314">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057213">
        <property type="entry name" value="DUF7891"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25421">
        <property type="entry name" value="DUF7891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="909"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="91"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="261"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="444"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="818"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="458"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="835"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="858"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6222696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1174" mass="129033" checksum="9F79085A6BA62991" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCA2</accession>
    <name>A0A8H6FCA2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Delta(14)-sterol reductase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">C-14 sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Sterol C14-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_000882</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152177.1">
        <property type="nucleotide sequence ID" value="XM_037291815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCA2"/>
    <dbReference type="GeneID" id="59329300"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050613">
        <property type="term" value="F:Delta14-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF52">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE TM7SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01017">
        <property type="entry name" value="STEROL_REDUCT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12 13" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8 13" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6222831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="491" mass="54798" checksum="6A33AAD06614C776" modified="2022-01-19" version="1">MSKSERKSVLPSEPSHGYEFGGPLGAFATSLGLPLVCYLATFLCNDISGCPVPSALHPWSLTVAKLKEETGWPGFQGLISTKVTVWVLGYYFLSLVLQAALPGVLTEGVELSSGGRLKYKFNAFSSAVTVIGAAALGTFVQGPDFPLWTFIWDNYTQLITVNLGVAFSLACFVYARSFSVKAGNKEQRELAPGGHSGNLVYDWFIGRELNPRVTLPVFGTIDIKAFCELRPGMLGWVLLNLAFCAHQYKVYGYVSDSIIFITFFQALYVFDALYMEPAILTTMDITTDGFGFMLAFGDLAWLPFIYSLQARYLAVFPVDLGYAGLAGLFAVQGFGYYIFRGANNEKNRFRTNPKDPKVAHLKYIETKSGSKLLISGWWGITRHINYLGDWIMAWSYCLPTGIAGYMVQQSSSLSDHPADGSIFGGTPTRSPVVQGEARGWGMAITYFYVVYFAILLVHREMRDEEKCMRKYGKDWEVYREKVPWRLIPYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCR1</accession>
    <name>A0A8H6FCR1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152889.1">
        <property type="nucleotide sequence ID" value="XM_037291454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCR1"/>
    <dbReference type="GeneID" id="59328934"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="577" mass="64583" checksum="474D8D56CE64E167" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCS9</accession>
    <name>A0A8H6FCS9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Solute symporter family transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_000438</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152812.1">
        <property type="nucleotide sequence ID" value="XM_037291377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCS9"/>
    <dbReference type="GeneID" id="59328857"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000006">
        <property type="entry name" value="Urea active transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF2">
        <property type="entry name" value="SOLUTE SYMPORTER FAMILY TRANSPORTER (AFU_ORTHOLOGUE AFUA_6G03200)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="496"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="589"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="621"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="532"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="563"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6223595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="666" mass="71906" checksum="8AD6AAEF69C18342" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCV5</accession>
    <name>A0A8H6FCV5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152887.1">
        <property type="nucleotide sequence ID" value="XM_037291452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCV5"/>
    <dbReference type="GeneID" id="59328932"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="273"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="676"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="692" mass="77953" checksum="FE79B5EDE957BC09" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDN0</accession>
    <name>A0A8H6FDN0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153301.1">
        <property type="nucleotide sequence ID" value="XM_037301667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDN0"/>
    <dbReference type="GeneID" id="59339206"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="297" mass="33105" checksum="346D84228CDFEC60" modified="2022-01-19" version="1">MGSTALRSKPTVHNGALHNMYKSQQSDRSRHDDGRRSPVSPIESNGETWKYPPRAQAHLKGLGPLHDKPHGEAPKDHFRSRPMSYYDGLPASPEPDTKAVFADLPAAPAKKRLSRPFEQPYPTSNQGRQAPGKGQTSTPSAAVPMQANLSRADSRLRRQPAGFSHPKAPAPLAIPPPAHQSRRQREPSPYREDQAPAQRSVRHQGKPVSGQRPARHQGWNPLQEPARQKGTVYVQRKDQRRGDDLERQGGLARSKSRAKDNDPEYKSGRRCFFSLMVMMILVIVVVVVMVEKNKSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE78</accession>
    <name>A0A8H6FE78_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010429</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154099.1">
        <property type="nucleotide sequence ID" value="XM_037301282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE78"/>
    <dbReference type="GeneID" id="59338820"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079:SF6">
        <property type="entry name" value="GH26 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="51984" checksum="C205ECC415109221" modified="2022-01-19" version="1">MAAEGFIPRGLLLDRGQGGVEDPLTSFHRPPYTFSLTACLSIESALCLAYASVFSEFLRQPKPCGFDLTGPEKGLSRVDDMILEATPAADELVLGHSDPLQLPPILINTRRLSFGSGMSAGKCSHTAHQKSLASLAGKIKVELSTLVILIIDGSCAAMLEGRDACGNDSALGGMQSSGGVYWGFLPDCCTGGDIMLMSTINGLTGKQASASDLVVIRQFNLVLTENSYGYNSQIRGLPYIGEQFDSIKGDIVQSGTVFIPAIMPTKVKFLDITSIVADQIAATDEFVTAWQKVHAAVANNSQTLMFWCPNYNTVENMQPCWPGAEYVDIVGMDDYPPAETAFASVYGAFCDGFAARYDKHFCIGETGSFNGGTVEAKEAWVSQISNVDLNRFPCYKSITWFEYLKASDDGSSEYDYRIIQGQLPAPIEQTLSNFKQLARLTRCVARALRFASLFVLSGKLGLFAPYISDNPFQQRDISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FER0</accession>
    <name>A0A8H6FER0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tafazzin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_010365</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a cardiolipin + a 1-acyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:33731"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
            <dbReference type="ChEBI" id="CHEBI:64743"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33732"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:33733"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the taffazin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154035.1">
        <property type="nucleotide sequence ID" value="XM_037301218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FER0"/>
    <dbReference type="GeneID" id="59338756"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000872">
        <property type="entry name" value="Tafazzin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497:SF0">
        <property type="entry name" value="TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497">
        <property type="entry name" value="TAZ PROTEIN TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00979">
        <property type="entry name" value="TAFAZZIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="14">
        <location>
            <begin position="43"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="325"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6225168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="360" mass="41022" checksum="5FD622AD9C2684A8" modified="2022-01-19" version="1">MGFVGTLTQAFMHGPNWQDAHGLDGFLELLDKRANVDGRQRGLITVSNHICVIDDPLIWGVLPLRYHFNPSNHRWSLGSYDIVFKNKAMSTFFVLGQVLPTHRLHKDPHGGPFQPTLREAVRLLSRPPFSPTSSALPVDRSLNSPDISDPFSSPNDHFTYTTDGIDTFPAPSAYATRQYSWIHIFPEGRVHQHPQKAMRYFKWGVARLILEPDVCPDIVPMWVEGNDEIMHENRAAPRWLPRAGKRCGVWFGENVGGESEGNVFHELRERWRRLVEDERRTTKADQELEVGVLSEELKYGPEAVKLREECTMQVRGAVLRVRKERGLPDEDPKEGLVETWRAEGGKREGQMDDGSLIKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFD5</accession>
    <name>A0A8H6FFD5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="1">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009830</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154537.1">
        <property type="nucleotide sequence ID" value="XM_037300689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFD5"/>
    <dbReference type="GeneID" id="59338225"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02662">
        <property type="entry name" value="Peptidase_C19F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF904">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="3">
        <location>
            <begin position="49"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="181"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="463"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="465"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="508"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="555"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="568"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="633" mass="70202" checksum="48155A950002A325" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFS7</accession>
    <name>A0A8H6FFS7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_008353</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Nibrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155221.1">
        <property type="nucleotide sequence ID" value="XM_037299220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFS7"/>
    <dbReference type="GeneID" id="59336750"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22667">
        <property type="entry name" value="FHA_NBN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10980">
        <property type="entry name" value="Nibrin, BRCT2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040227">
        <property type="entry name" value="Nibrin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032429">
        <property type="entry name" value="Nibrin_BRCT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043014">
        <property type="entry name" value="Nibrin_BRCT2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12162:SF0">
        <property type="entry name" value="NIBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12162">
        <property type="entry name" value="NIBRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16508">
        <property type="entry name" value="NIBRIN_BRCT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="7">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="425"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="522"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="549"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="608"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="634"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="728"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="742"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="795" mass="89859" checksum="05D07F08E48F7D5A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFU8</accession>
    <name>A0A8H6FFU8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155119.1">
        <property type="nucleotide sequence ID" value="XM_037300293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFU8"/>
    <dbReference type="GeneID" id="59337827"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="331" mass="38247" checksum="747DBE11C9B78DF6" modified="2022-01-19" version="1">MEPGLKSFLMGVPLEVRIKILEHLLPNLSEIKVFDGYYDSFNPVNGYDIWMGADKVITNRIASVVQYRDDGEKCHTAILRLYIEGTAVMYNRAFRAVITARGLTFLKCLLSSSEWCYKWGNIPVPSELRHFPFHMAKQVQIEFWATRFKKEEPILYDTLLGFCLVLCNKPSLRNVRVDLYDREYRPKPVLRNGDRMADGSRPNPPQHVYGGEVLDGPFQRTEEEIEFWSDVQRRDFYDLDAEPWERAAGRDFRSWQARGRELRGLPRISGLEEALEPLKLLAHVGRAKINLTPGAGNDPEMVAVAEKLQDSMMHQGLRHEMDLTDLRLTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG06</accession>
    <name>A0A8H6FG06_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155248.1">
        <property type="nucleotide sequence ID" value="XM_037299247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG06"/>
    <dbReference type="GeneID" id="59336777"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="248" mass="26503" checksum="1E1987A4C8AB69B5" modified="2022-01-19" version="1">MDFQQAPSREAYLVLDAIANFNNYLEALVTQFSYTTVQPASTSRILEILNDRAAGLLILLTIASVAVPLLGELTIAGASVAATTQAVGAGAGAAAGVQSGILPAVANALPSAHALVNPNAYNVEDVSSFMLQQYVFNAINFLWKQEKTYILAADSSDCGNDQRGPQESYIVLPRDQIPNIGTEYKGVIKEGDLFDKGDEPAREDWGEDGRGVEDAVDTILPEGGAVGLYKLEIRVLCRVNGLREDARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG28</accession>
    <name>A0A8H6FG28_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009415</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155102.1">
        <property type="nucleotide sequence ID" value="XM_037300276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG28"/>
    <dbReference type="GeneID" id="59337810"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF47">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="519"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="553"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="585"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="179"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="624" mass="68578" checksum="A309747F2B18CF1A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG30</accession>
    <name>A0A8H6FG30_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zinc metalloprotease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008669</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155535.1">
        <property type="nucleotide sequence ID" value="XM_037299534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG30"/>
    <dbReference type="GeneID" id="59337064"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000015">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000031">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016:SF16">
        <property type="entry name" value="METALLOPROTEASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G07610)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016">
        <property type="entry name" value="PRESEQUENCE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="2">
        <location>
            <begin position="63"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="3">
        <location>
            <begin position="203"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1027"/>
            <end position="1054"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1054" mass="117574" checksum="F58B41DB0B232FAF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG35</accession>
    <name>A0A8H6FG35_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA-capping enzyme subunit beta</fullName>
            <ecNumber evidence="8">3.6.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-triphosphate monophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_008471</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67004"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
            <dbReference type="EC" id="3.6.1.74"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:67005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the fungal TPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.74" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155338.1">
        <property type="nucleotide sequence ID" value="XM_037299337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG35"/>
    <dbReference type="GeneID" id="59336867"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07470">
        <property type="entry name" value="CYTH-like_mRNA_RTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.10">
        <property type="entry name" value="mRNA triphosphatase Cet1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040343">
        <property type="entry name" value="Cet1/Ctl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004206">
        <property type="entry name" value="mRNA_triPase_Cet1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037009">
        <property type="entry name" value="mRNA_triPase_Cet1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118:SF1">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE CTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02940">
        <property type="entry name" value="mRNA_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA triphosphatase Cet1-like" evidence="10">
        <location>
            <begin position="577"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="295"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="382"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="215"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="413"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6227030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="874" mass="95975" checksum="3C75A76B2EDA6552" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG48</accession>
    <name>A0A8H6FG48_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_008730</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037299594.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AA-permease/SLC12A_dom"/>
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    <dbReference type="InterPro" id="IPR004840">
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    </dbReference>
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        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
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        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="444"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="491"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="535"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="96"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6227287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="602" mass="64894" checksum="514FE2F809E960B8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG85</accession>
    <name>A0A8H6FG85_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPI inositol-deacylase</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">HO133_008412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155279.1">
        <property type="nucleotide sequence ID" value="XM_037299278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG85"/>
    <dbReference type="GeneID" id="59336808"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052374">
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    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182:SF3">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
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        <property type="match status" value="9"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1168"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="790"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1182"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1204"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1224"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1244"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1257"/>
            <end position="1272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1278" mass="144666" checksum="0ECDD055C8E0B455" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGX0</accession>
    <name>A0A8H6FGX0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155712.1">
        <property type="nucleotide sequence ID" value="XM_037299711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGX0"/>
    <dbReference type="GeneID" id="59337243"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="90" mass="9762" checksum="27B29D116D91F8FB" modified="2022-01-19" version="1">MAEKGSAEPVWTHWLTSVSTGRVVVEMSNQEPGEESLFKVIGNIIIVNLMETVAEGQVLAEKTGLKIGNLHSCLSTKPGEKIVDCMPTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH43</accession>
    <name>A0A8H6FH43_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007793</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155999.1">
        <property type="nucleotide sequence ID" value="XM_037298663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH43"/>
    <dbReference type="GeneID" id="59336190"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03752">
        <property type="entry name" value="proteasome_alpha_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000009">
        <property type="entry name" value="Proteasome subunit alpha type-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="251" mass="27782" checksum="E76DD74DD3C77C92" modified="2022-01-19" version="1">MSRRYDSRTTIFSPEGRLYQVEYALEAISHAGTAIGILSTDGIVLAAERKVTSKLLEQDTSAEKLYILNDNMICAVAGMTADANILINYARQAAQRYLLTYNEDIPCEQLVRRLCDLKQGYTQHGGLRPFGVSFIYAGYDPQRQFQLYQSNPSGNYGGWKATSVGANNASAQSLLKQDYKEECDLKEACGLAVKVLSKTMDSTKLTSEKIEFATVGKTKDGKVYHHLWGADEITTLLQEHGLAKAEEEDTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHE6</accession>
    <name>A0A8H6FHE6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MICOS complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007961</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156165.1">
        <property type="nucleotide sequence ID" value="XM_037298829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHE6"/>
    <dbReference type="GeneID" id="59336358"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019166">
        <property type="entry name" value="MIC26/MIC27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033181">
        <property type="entry name" value="Mic26_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28268">
        <property type="entry name" value="MICOS SUBUNIT MIC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28268:SF1">
        <property type="entry name" value="MICOS SUBUNIT MIC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09769">
        <property type="entry name" value="ApoO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="210" mass="23696" checksum="8B7D30A26407BBB8" modified="2022-01-19" version="1">MTFRPLLRQLPRKPIYDDLALLDPPKSSPAPKEDSLPSSPTPTDRLAGQIKHARLFIHAHTVRTEDRLNNFMSTVLHQEHSFTQTIASLAPPPETHERVMPGALYVLVAAMTGSIISRNRNILLRATVPFAVGLGAAWIVLPVTTRNVGELVWTYEEKAPVISINHMRVRGAIEEGWRQAKIREEATKKWFDERVGESREAVEGWVRKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHE9</accession>
    <name>A0A8H6FHE9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007716</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155922.1">
        <property type="nucleotide sequence ID" value="XM_037298586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHE9"/>
    <dbReference type="GeneID" id="59336113"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF18">
        <property type="entry name" value="HEAT SHOCK PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="301"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="784" mass="87958" checksum="60585C9DDE128896" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHG2</accession>
    <name>A0A8H6FHG2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155878.1">
        <property type="nucleotide sequence ID" value="XM_037298542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHG2"/>
    <dbReference type="GeneID" id="59336069"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034021820" evidence="1">
        <location>
            <begin position="19"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="286" mass="32218" checksum="8DC502AF38E3781E" modified="2022-01-19" version="1">MSPILTFLALLLASLTAGLPIHQSSQNTTTHLDPVASGISTHALAKRTTANPPFDPWNKDKPEHRADDEFEGDIFRLSKCYCIDTPSRDLPAVYGQFYGSYYGYDYYNYHQNKSYAFNMTCASGELESLNNNLNRFDYERYLLAPKCLSWMEEERKQCWSTGDGNEFCAEAMHGKDNYYWNGQKRKVHAHPSAIGTIKMDAAPLVAECQRMCQTVPARTEGYMQDTLVSDYEHKINLATATTCSGSSGVGMGLLCEGKNKVVPKVWDFWNYIETYSDQADMCKGCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHJ5</accession>
    <name>A0A8H6FHJ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclin-dependent kinase 1</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_007866</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a key role in the control of the eukaryotic cell cycle. Required for entry into S-phase and mitosis. Acts as a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. May function in concert with npp-16 to arrest prophase blastomeres in response to anoxia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cks-1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156072.1">
        <property type="nucleotide sequence ID" value="XM_037298736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHJ5"/>
    <dbReference type="GeneID" id="59336263"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07835">
        <property type="entry name" value="STKc_CDK1_CdkB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000706">
        <property type="entry name" value="Cyclin-dependent kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000027">
        <property type="entry name" value="Putative Cyclin-dependent kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF254">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="4"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6228138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="325" mass="36428" checksum="D6ADACD9BD1FFA98" modified="2022-01-19" version="1">MENYQKIEKIGEGTYGVVYKARDLTHPTRIVALKKIRLEAEDEGVPSTAIREISLLKEMNDPNIVRLLNIVHADGQKLYLVFEYLDLDLKKYMEALPISDGGKAKAFPEGSGPDLGRLGLGEAMVKKFMSQLIEGVRYCHSHRVLHRDLKPQNLLIDRDGNLKLADFGLARAFGVPLRTYTHEVVTLWYRSPEILLGGRQYSTGVDMWSVGCIFAEMCTRRPLFPGDSEIDEIFKIFRLLGTPTDQDWPGVTSFPDFKPSFPKWSRDPSRALVNGLDDNGLDLLDAMLVYDPAGRISAKQACTHPYFEAGSSAYSGRGRGTNGFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIR7</accession>
    <name>A0A8H6FIR7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">18S rRNA factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007397</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the small subunit (SSU) processome assembly and function, and in the 18S rRNA synthesis. Required for the early cleavages at sites A0, A1 and A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF2/ABP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156923.1">
        <property type="nucleotide sequence ID" value="XM_037298288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIR7"/>
    <dbReference type="GeneID" id="59335796"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12263">
        <property type="entry name" value="RRM_ABT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039119">
        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034353">
        <property type="entry name" value="ABT1/ESF2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="267"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="267"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6229281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="329" mass="36912" checksum="D365081A23ECC2DD" modified="2022-01-19" version="1">MAVRKHNEFLDTNLSEDDENVSSSSEAAADSRTAGLASHHSKRQKLTVEHSDSDGETDNDTQDDAAPITSPAFPKKPSRSSAASTTKPTEPISTDHPTEFPEKRKPLTTSAVAAANQRAHKAGVIYLSRIPPFMRPSTVRTLLSAHGTITRLFLTPEAPSSYLSRRKAGGNKKHSYVDGWVEFSRKRDAKVCVEAINGRIVGGKKGGWYRDDVWNAKYLKGFGWGDLMAGVRGEEREREERVRVGVGREGRERAEFLRNLERGKVEDTRRMKRERRAEREGGGKETGVGKEREKTSGFERRFRQNEVKGREEKGVEQSDETKRVLSRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIS2</accession>
    <name>A0A8H6FIS2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007364</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156890.1">
        <property type="nucleotide sequence ID" value="XM_037298255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIS2"/>
    <dbReference type="GeneID" id="59335763"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF146">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="38"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6229248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="395" mass="43287" checksum="B8E8120CD910B7E2" modified="2022-01-19" version="1">MATTSKAASSSDPSNKGGTAYLATQSTFVAIATLLVLIRVYVRTVVVKSIGLDDAVIVLALILSLIVCAMTSLTVHYSALYLAGDYSTKAEALRDVTTALKWSSIAAPIGIFSGLCTRLSICLFLLRIFRTKREWRWGLYAVMVFATAVIIPTIVSLLAQCSPVQKLWDPLLPGSCWSPRTVIDIGYFNGGASVLCDWILATLPIVFMWNIQMRFTVKIGICILMGLGYFTGICAIVRTTLFTDGTSGAEQGGSIQSAVWAILEINVGIIAASIPTLKPLFNPSRPSIFRYFKYRRTSERYRKTSENTLAIDTDDQVQLNKLTLNTMPSAMPSSLSYYQDKGRNHGKGWEDLESQASMSIPEKVHLAERGREMETGSNLEYVGDGEVHAIGEYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKG6</accession>
    <name>A0A8H6FKG6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="3 11">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004522</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="3 11"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157440.1">
        <property type="nucleotide sequence ID" value="XM_037295439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKG6"/>
    <dbReference type="GeneID" id="59332930"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000015">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000111">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 11" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="12">
        <location>
            <begin position="26"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="205"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6230183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="511" mass="58769" checksum="6355ABE7326132EA" modified="2022-01-19" version="1">MADTMIHPDEPEDAGTIELKDNTIIVVLGASGDLAKKKTFPALFGLYRNRFLPKDIRIVGYARTKMDHEEYLKRVKSHIKVPTKEMEEQLEEFCKICTYVSGQYDQDEPFKVLNEHLEGIEKGREEQNRVFYMALPPSVFITVSQHLKRNCYPKRGISRIVVEKPFGKDLGSSRELQKALEPDWKEEEIFRIDHYLGKEMVKNILILRFGNEFFGATWNRNHIDNVQISFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQVLTLLAMERPISFSSEDIRDEKVRVLRGIPNIEPKNVIIGQYGKSLDGNKPAYKEDDTVPKESRCPTFCAMVAYIKNERWDGVPFILKAGKALNEQKTEIRIQFKDVTSGIFKDIPRNELVIRVQPNESVYIKMNSKLPGLSMQTVATELDLTYRRRFSDLKIPEAYESLILDALKGDHSNFVRDDELDASWRVFSPLLHYLDGNTEITPMEYPYGSRGPAVLDDFTSSYGYKFSDPAGYQWGGAQNEANKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKU8</accession>
    <name>A0A8H6FKU8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ADP-ribose 1''-phosphate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004723</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Highly specific phosphatase involved in the metabolism of ADP-ribose 1''-phosphate (Appr1p) which is produced as a consequence of tRNA splicing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ADP-alpha-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:58753"/>
            <dbReference type="EC" id="3.1.3.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.84" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157638.1">
        <property type="nucleotide sequence ID" value="XM_037295637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKU8"/>
    <dbReference type="GeneID" id="59333129"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140291">
        <property type="term" value="P:peptidyl-glutamate ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02901">
        <property type="entry name" value="Macro_Poa1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050892">
        <property type="entry name" value="ADP-ribose_metab_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521:SF0">
        <property type="entry name" value="ADP-RIBOSE GLYCOHYDROLASE OARD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521">
        <property type="entry name" value="PROTEIN C6ORF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="8">
        <location>
            <begin position="21"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="182"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6230381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="261" mass="28197" checksum="F2E44D20566AC32A" modified="2022-01-19" version="1">MAIITEEVGDIFSAPPNSILIHACNARGAWGSGVAVAFKQKYPYAFRQHQAYCLSSPTGSKLSVKQHQASLVGTALLIPPPTATNQRQASKTPHYIACLFTSLDYGKRVSPPEEIVENTRKALENLAKQVAEMRAAGEEVGKCCPVRINSGRFGVEWQKTKAVLEAGGLVITVVRPEAEQKADGAGTAAGGQSPVKDENEKAKGEKCAVGTNARRSNRWTSGRTKAKGPFEKQTRGVKRKVEKDFEDGDDGREDMKGERLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKV2</accession>
    <name>A0A8H6FKV2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004734</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157649.1">
        <property type="nucleotide sequence ID" value="XM_037295648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKV2"/>
    <dbReference type="GeneID" id="59333140"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046359">
        <property type="term" value="P:butyrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="6">
        <location>
            <begin position="12"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="5">
        <location>
            <begin position="256"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="426" mass="45475" checksum="B07EE1D71085EC39" modified="2022-01-19" version="1">MPVDFHLSPPEEGIRKAAAGFTAGPLKDAKKTYMQYQHHHEKFQSTRPLYHQAVAGGLIKGQIPPRLGGAGGSLMEAVLLVEEMYRVEPAASLTIFSTGLGLTPMNLTGKPEHKEFLEPFLSGKGEPLASMVFSELGGVANWLEKGAPGLNTTATLEGDEWVLNGEKIWATNSAGWDFEGADLQCVVCRVANPSSDEPADAIMILLVTIADIRHNALGAFKVLRHVPTAGHTACSGPHIKYTNLRVPAKNVLCPPGTGAPIVSGAFDCTAVPVGAMSVGIMRAAFDAALAFAKSDNRRGAADLLTRQAVADLLSGIKMQTEACRALTWKAANCLENGPGDYNARRELALAAKIYCSDACTKVVTEAINVVGITAYDADQPFADLLNTAMVLPIFDGGNVGIRRRHMQELMLSKDYDAWASTYGPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL47</accession>
    <name>A0A8H6FL47_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004888</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157801.1">
        <property type="nucleotide sequence ID" value="XM_037295800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL47"/>
    <dbReference type="GeneID" id="59333294"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF28">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="1"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6230544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="418" mass="46587" checksum="BBCECA8373CD898F" modified="2022-01-19" version="1">MIVGSLLQSTAYTRAHIIVARIVSGCGMGFINSTVPVFQSEFSPKASRGLYVCMQLSTLNFGTDLVYWIDYAFSTHTSSYAWRIPVILQCVFLFPMLLIIFVLPESPRWLAAHNRSDESLSVLRRLKGHTLDDEAIVRLHSDIVRAVEEEAAMNAGSWRTILSADKIHSRRRLLISSAIQAFQQLGGINAIIYYSSTLFKNVGFSNHLSALMSGFLQLWFFVASFIPWLLIDRIGRRPLLLSMITVMALVMAAQAALVYQVQFKTSVAYAAGIAGAVMLFLFEGAFTIGFQATVWVYPSEILPLRLRQKGSSISTAANWICNFLIVQITPPAIENVGWRTYVIFAVLNATWVPIIYLFFPESKGRELEDFDRMFAGEDDAAITETTTGDKDPVYLERNEPVAVKGAAAHEEEGLKSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYE2</accession>
    <name>A0A8H6KYE2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rho GDP-dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007238</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rho GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156766.1">
        <property type="nucleotide sequence ID" value="XM_037298131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYE2"/>
    <dbReference type="GeneID" id="59335638"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005094">
        <property type="term" value="F:Rho GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.30:FF:000001">
        <property type="entry name" value="Rho GDP-dissociation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.30">
        <property type="entry name" value="Coagulation Factor XIII, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000406">
        <property type="entry name" value="Rho_GDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024792">
        <property type="entry name" value="RhoGDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980:SF3">
        <property type="entry name" value="LD16419P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02115">
        <property type="entry name" value="Rho_GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00492">
        <property type="entry name" value="RHOGDI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6229124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="200" mass="22461" checksum="AECF156F7D87D31F" modified="2022-01-19" version="1">MASQGEDDLQASTTEGFKVGEKKTVEEYAKLDQNDESLNRWKASLGISSSPAIHVDPKDQRRCVIKSLALEVEGRSDITIDLTAPGSVEALKSKPFTIKEGAKFRMKANFVVQHDVLSGLKYLQVTKRKGIRVAKNEEMIGSFAPNTQDKPVYEKKFEPDEAPTGMLARGHYEAASKFADDDGHEYLKFEWSFDITKEWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZJ2</accession>
    <name>A0A8H6KZJ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157220.1">
        <property type="nucleotide sequence ID" value="XM_037295219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZJ2"/>
    <dbReference type="GeneID" id="59332709"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="166" mass="18725" checksum="006F28CBF0BEB167" modified="2022-01-19" version="1">MPDYKAKGELMRSTAYSFCKAFTTGSPPSETLDKYFTSNPKILEHGPKWANLRLPFLGIKFHGRRSQGSSTATSRRTCDDYYDRLTSTLSFHPYENTVPPKEEFMVDAEGGTVTIKLRAKFASVKTGKDWEEDFVYVLSEFDEEGKIGSQELWADPLSAWVAVGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZN7</accession>
    <name>A0A8H6KZN7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004437</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157355.1">
        <property type="nucleotide sequence ID" value="XM_037295354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZN7"/>
    <dbReference type="GeneID" id="59332845"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043504">
        <property type="term" value="P:mitochondrial DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001238">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000010">
        <property type="entry name" value="DNA mismatch repair protein Msh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF34">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="8">
        <location>
            <begin position="880"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="575"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6230098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1007" mass="111202" checksum="26AFF4C77AF02938" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L031</accession>
    <name>A0A8H6L031_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157806.1">
        <property type="nucleotide sequence ID" value="XM_037295805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L031"/>
    <dbReference type="GeneID" id="59333299"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="94" mass="10798" checksum="C2E182A2D41A6731" modified="2022-01-19" version="1">MGSHVNELTRFEGLADLSAEKLQQLETVFKSILALPITWVTYAQIIDRERSYVDPLRKLPTKVADVSDHLNPSDGATHYHEEFWKVFTARAREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C4Q9</accession>
    <name>A0A8H6C4Q9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000028">
        <property type="protein sequence ID" value="KAF6217427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146862.1">
        <property type="nucleotide sequence ID" value="XM_037297793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C4Q9"/>
    <dbReference type="GeneID" id="59335297"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="104" mass="10131" checksum="DB1B768D45BB77DC" modified="2022-01-19" version="1">MLIPSWLFASASRSSSQVSGRRSCNSVSELDEGAGAGTGCGVAGLEELGSGVGNGCVAGNEVAAASLGKVVAVVASGGLDLGVLAVASSSFCRFLGELRPVLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C4X5</accession>
    <name>A0A8H6C4X5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000028">
        <property type="protein sequence ID" value="KAF6217422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146857.1">
        <property type="nucleotide sequence ID" value="XM_037297788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C4X5"/>
    <dbReference type="GeneID" id="59335292"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="349" mass="39240" checksum="86E4AF03136020AF" modified="2022-01-19" version="1">MYAMATKASNSSTSTVDSETSFTVSLPGLEIATEVVSDLEFSIPHYRVMTFSRPLWDSWTCQQVGRPLKTLQIEIRLEIYGYFLLAPPASSPRTNETSYKGLRSALPITLSPKSWTVGGLPIASFFRLYPAILSTCRAIHEEATEVLYGKNLFQFWVPYEANNEIGGNISILEQSENVFKFNNKDWRWRSDGPLLLRDSTFACFLNSIGPGNAASLTAIEFVAPCGENNKYQFQLMTKLLARHVLGLKFIRVSNGFEWPMDGDWPRCLAPAGKDEELHQTCRTLTDLVRGCPSLEEFEYRGSFIKPDSELEAKTGCLGKISRLLEERKAKNAPRMVTSQEKEKGDETES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5A1</accession>
    <name>A0A8H6C5A1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146948.1">
        <property type="nucleotide sequence ID" value="XM_037297747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5A1"/>
    <dbReference type="GeneID" id="59335251"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034864873" evidence="1">
        <location>
            <begin position="20"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="306" mass="33889" checksum="6B4286CE34109F02" modified="2022-01-19" version="1">MPVFQFLTIAAGFMVLVSAGRIGNRQGDVPIFTCAKTCLRDSHLRNNRGRRLSFHGCWIARYDVLEDGIYLCSYPNYTTVLNDESTFVDAAGWMDDYGHKQLLQQRPVIYYPESITDLWTLSDRKQLRFLGVCLLSTPIFPLRHNHANILLGPEMDDEQYLTASSSINGFENDEVAFNGCNCVTTWGPVPTSSLQISQSGFGGPGYCGGPICEVSPSTIPVGQSTRLRGGLGTLVEFQLLPVTARKRGLAFLGLIQQAWLGLVRVLKLGWLPSILESPTEMVKYSHQIKTPQLLTANGANKLEREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C600</accession>
    <name>A0A8H6C600_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146964.1">
        <property type="nucleotide sequence ID" value="XM_037297763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C600"/>
    <dbReference type="GeneID" id="59335267"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="261" mass="28007" checksum="86B4B0E47922DBF9" modified="2022-01-19" version="1">MGNSTSLHVALFGGVPDRKKQAWVRPKAPRSENNGQAGPSTSNYPGPPGKMQEYDDEAGLYDSSHPPPDSKGPPRPHTAQPHTPGPEAGHSSPTGGPKPQHPSRMPTYHVEDEEFPNHTPGPGPRLTGSSQVGPPIPQAQPPKRQKQPPHSRRPSLTKPRSSSPHSRPPGDRSRQHPPPPPRHSHSAAPHPHSHTPHSAAPNPHPHPRSRTPPSAPPHPHGRHAEGGGGERGGMRHPRDSGDAVRDPRRITGRSRRGEEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6G0</accession>
    <name>A0A8H6C6G0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HhH-GPD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006849</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146946.1">
        <property type="nucleotide sequence ID" value="XM_037297745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6G0"/>
    <dbReference type="GeneID" id="59335249"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045138">
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    </dbReference>
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        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN PROTEIN 4-LIKE PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="756"/>
            <end position="830"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
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        <location>
            <begin position="333"/>
            <end position="361"/>
        </location>
    </feature>
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        <location>
            <begin position="412"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="412"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="465"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="496"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="536"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="574"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="597"/>
            <end position="617"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6217511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="989" mass="110583" checksum="9107A084BC374559" modified="2022-01-19" version="1"> </sequence>
</entry>
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    <name>A0A8H6C6K7_9LECA</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <begin position="1"/>
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        </location>
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            <begin position="24"/>
            <end position="250"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6217539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="26722" checksum="B806DA4D66E74BD4" modified="2022-01-19" version="1">MKSLFSIGSGLVTLLLATNPTNAEIPVWEFGEEPVGSKTCIGCKQCYVESNFAEMPFGVTIGNASCVDLNGRMGAGTEYGTLIVDFSPTVPGATGMYMDLFDGTWIGGIAWSKANGVETNITLKGTGQWGSASYIGGWCVANIVQHQKVNAAADATVPEGQYSYDIELFDNGTPRNWIGGTKGGPLYVQDGKTQGLDSLLPQVFEITSGNVDDDPYTMSYNNPYLPSPSNEVWSASTRQSVQFLGARTTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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                <person name="Walston R.F."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6L7</accession>
    <name>A0A8H6C6L7_9LECA</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Letharia lupina</name>
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        </lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6217872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147307.1">
        <property type="nucleotide sequence ID" value="XM_037297182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6L7"/>
    <dbReference type="GeneID" id="59334685"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="271" mass="31488" checksum="541881434853C8D2" modified="2022-01-19" version="1">MSDLDQVPQQIYGLDRRTSRVPGNAVYKPVASYDQMLAFLVKIRSTLAKPSLDNMNYILANQEPLHEVRLAMKRLSENFTRAALACDDAKRCTEEVARRFQQAWDLVDVKANATANQSRYVNNISQEIAEVWRTHTSAPNKHWKIVFASLVYELKVAIENRQRMRWGLEHAEKRAEQWEARYILAVDTWCNTYQDALHLEEAFTALRTEFLAGQSLAEIFMRETREIGDCISVRSESERVCRIDQALIREEERDTKNYGVMSRFQKYSRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Z7</accession>
    <name>A0A8H6C6Z7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147565.1">
        <property type="nucleotide sequence ID" value="XM_037296991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Z7"/>
    <dbReference type="GeneID" id="59334493"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="212" mass="23492" checksum="97EEE5BA63DAE082" modified="2022-01-19" version="1">MPHPTPLASLQEQMTQMMEMIRIMSTRLEALEARSRPTQPATQPAHQVTQQATPSVAQSDTSQEEDIAEKSAETVSSSSDTTDLLSTISHQPDTQKEEDPLQANAESTAEKSSDSKAPSTKNTAQLSKIPRHMLSTVPHQTITQQAENTTAAWPIIPTTSLWHTITELSIEEKDLLRRSQPNHYGYGNWTSAGKGLFRRKIGRCIFGRIETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C765</accession>
    <name>A0A8H6C765_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147592.1">
        <property type="nucleotide sequence ID" value="XM_037297018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C765"/>
    <dbReference type="GeneID" id="59334520"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034949855" evidence="1">
        <location>
            <begin position="27"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="280" mass="30554" checksum="86DA872A638E2346" modified="2022-01-19" version="1">MSPMALIKLGVLPISVIFGCSLLANAAPHDINSAADDAALIISLNPLDPEAPLPDPARGGTPRSIDSPLLVTEVLGFSKSAAFFEMLVELRLMIYEHLIEAGDLGILTCCKESLGTTGTESLGVLLDFWDQSVVTEEGFKWVYTGKSVYMICKFYANHQKSDGFWDHVNWIRAPTRANVGYSPTSSSLDTNGWTSSTKIDPSTPGWDTLKQIRTAQLPTINIGTPSIHWRLDFDAAQSSGLTCSGCLRSWSDAERCGKRFPTRKIPLSFTSLIMSLEMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7A8</accession>
    <name>A0A8H6C7A8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="3">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_006178</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147652.1">
        <property type="nucleotide sequence ID" value="XM_037297078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7A8"/>
    <dbReference type="GeneID" id="59334580"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01414">
        <property type="entry name" value="SAICAR_synt_Sc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010568">
        <property type="entry name" value="PRK13961.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="10">
        <location>
            <begin position="17"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="312" mass="34546" checksum="9C258480D85DAE66" modified="2022-01-19" version="1">MSSQAVISIDLKSLKLVASGKVREIYEIDKSTLLMVVTDRVSAYDEILSSGIPDKGAILCQMSAYWFTLLGAQMPNFKHHVLSLKPPSGCQIVSPSERATLRGRCMQIQALKVFPIEVIVRGYLTGSAWDEYKKSSTVHGIPQPAGLQRCQALPGGPIYTPSTKAPPGFHDENISPEEARKIVGEKWAGEIENLALSIYKTAHSFAMERGIIIADTKMEIALDEKNDEIVVVDELLTPDSSRFWPAPVRVGEEQPSFDKQHIRDYLKAHGLGGKPDVELPEHIVTETTNAYREVFRRLTSQTLEQAMEALGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7H0</accession>
    <name>A0A8H6C7H0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147633.1">
        <property type="nucleotide sequence ID" value="XM_037297059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7H0"/>
    <dbReference type="GeneID" id="59334561"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034657429" evidence="1">
        <location>
            <begin position="20"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="310" mass="30311" checksum="49B7C854542CB3AC" modified="2022-01-19" version="1">MQSAILVLAAAAGVANAWAGNYSMSAGSAAGPSYTTTTLVDTTTVSCGATGTTITGSSKTFTLTAFETSTITDCGCTKTTVIPATPYTTTTTDIFTSTTVFCPAATTAIFGSKTYTASVNETLTITEGCPCTETKSVVYTPGVPVTTTGLTVSTTLVSTSTVVCPSSTTLLVTAPSTYVSVCPSPTTFTAVVNSLSTATHIASVSGQTITVTQSASVGTYTATPSQTVTVSEGPFSSSTVVPFTTTITPAPAAAEAGATTTAPAAPAGTAVGSVVAPKPSTAAPFKGAANKLSGAGAGVAGLLGLAAYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7M8</accession>
    <name>A0A8H6C7M8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147908.1">
        <property type="nucleotide sequence ID" value="XM_037296727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7M8"/>
    <dbReference type="GeneID" id="59334227"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029196">
        <property type="entry name" value="HAPSTR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645:SF1">
        <property type="entry name" value="YALI0F12243P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15251">
        <property type="entry name" value="TAPR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="274" mass="29636" checksum="124AD5FC51297AE2" modified="2022-01-19" version="1">MDSMRTLNTSLPRSPRRKRANQPPEQLIQSFKSAALSVTNLYKTAAADQTRSRDAGYQDALDDLLTFLDQQHLGLGDGEGWKVRQWATERLDGSPPAHMGSDSDDDRGETTKRARSSSPTAQRSTVPEGEQARQPSRSTSPVRAASVPMAPPATSQPQNNLFHAPEVFSFRSAHPYPYDVDMQAADTTSNTIPQPDPLPSPAVRVDVVSRGSRTPHRGGNHQNRHGARSTAGAKSLGSVAGSKRRITFGDYFDLGSLGDGKDGRDGGGKRGRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8N4</accession>
    <name>A0A8H6C8N4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Pre-mRNA-processing protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005480</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SNW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148372.1">
        <property type="nucleotide sequence ID" value="XM_037296390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8N4"/>
    <dbReference type="GeneID" id="59333886"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="5">
        <location>
            <begin position="188"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="216"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="310"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="542"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="356"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="377"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="562"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6218937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="577" mass="64707" checksum="7BDC472F07747502" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C914</accession>
    <name>A0A8H6C914_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005033</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148643.1">
        <property type="nucleotide sequence ID" value="XM_037295945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C914"/>
    <dbReference type="GeneID" id="59333439"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF1">
        <property type="entry name" value="TRANS-ENOYL REDUCTASE STHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="5">
        <location>
            <begin position="25"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="386" mass="40564" checksum="1FCB255DB5B189F6" modified="2022-01-19" version="1">MPLPINNPTTPPPPTQTAILTLATGALAICPTHPTPPAHPHHNTLLVRTHALALNPSDHKMPTRLRTPGLAAGCDFAGTIAAVGSDPDAPARPGCAPWAVGDRVCGAVHGCNVLRPGAGAFARWVEADPVVLVRMPRGWGWAEGAALGGSCVGAVGLGLFREMGLEGPREGGPGGEAAGLGRTVLVYGGSTACGTMAVQLLRLAGYIPITTCSPHNNAQATSYGAAATFDYRSPTCASDIKAHTQNSLRFALDCIGTAQSTALCHEAIGRSGGRYVALERYPESAAGRRNVRGSWVLGPVMFGRRIEMEGYGRDADAGAREFGREWYACVERLAREGKVRVHPPRRLGGRGGWARAVLEGLEMMKMGKVSGEKLVVEMDEQVDEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C924</accession>
    <name>A0A8H6C924_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004914</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148524.1">
        <property type="nucleotide sequence ID" value="XM_037295826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C924"/>
    <dbReference type="GeneID" id="59333320"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="13"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="322" mass="34371" checksum="15C27370B7313412" modified="2022-01-19" version="1">MASNRAAWQNAVGEKLNVQSAPYTPPSENEIVIKNQAWAVNPIDWLVQENALFDWMEYPLITGSDIAGEVVEVGSGVMRFKKGDRVLAMSIGQDKNKHSEGAYQLYSVAQTVLAAPIPDSMSYAEAAVFPLGLCVAACGLFQQDQLALQHPTVPPKPTGKTIIVWGGASSVGSNAIQLAVAGGYEVFTTASLKNFDHLQKLGASKISDYNSKTVVDDLVSALKDKHFEVVDKATGTKFVSSAPPIPENKPGGVEANMVWGSSLKDNEVGPAIFVDFLPKALAEGKYIPAPEPWIVGKGLESIQDGFEAQKKGVSAKKVVITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9M7</accession>
    <name>A0A8H6C9M7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Eukaryotic translation initiation factor 2A</fullName>
            <shortName evidence="8">eIF-2A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005103</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the WD repeat EIF2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148713.1">
        <property type="nucleotide sequence ID" value="XM_037296015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9M7"/>
    <dbReference type="GeneID" id="59333509"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000596">
        <property type="entry name" value="Eukaryotic translation initiation factor 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017222">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="10">
        <location>
            <begin position="218"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="505"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="571"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6219278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="697" mass="76220" checksum="33BCC15945D72470" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAM6</accession>
    <name>A0A8H6CAM6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003561</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lcsJ thioesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149171.1">
        <property type="nucleotide sequence ID" value="XM_037294483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAM6"/>
    <dbReference type="GeneID" id="59331972"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051490">
        <property type="entry name" value="THEM6_lcsJ_thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475:SF4">
        <property type="entry name" value="PROTEIN THEM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="353" mass="39445" checksum="5978BAB0E7D32E3F" modified="2022-01-19" version="1">MAPTLSTLRSKLGFAGKAGVWKFLALIFALLNLKSLPFVWHIRLLKGLFAQRRTSRLRLTTTPGPSTLFQPLITSSRSGFLECDYNLHKSNSTYFSDFDVGRLELLVSLCSHGLDKTRKELALEEKEGRGDGAMGKGRFDIRLGGVSCQFRKEIKPYEKFEVWTRVLCWDRKWFYVISHFVKPGVFRPKSYMLQPWRNGRRQKDHDAEIDGIVANGNTTPSPHPAIFATGIAKYVLKKGRLTIPPERVFRASELLPPKPADYQIPSATPTSTPESTSNTAAAVSIVDHITPGNTEDVVVASLKAGVGNEDGEWTWEKVEEERKRGMKIAEMWGALDGLNEEFAGDGGVVLGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBG2</accession>
    <name>A0A8H6CBG2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149862.1">
        <property type="nucleotide sequence ID" value="XM_037293784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBG2"/>
    <dbReference type="GeneID" id="59331271"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040632">
        <property type="entry name" value="Sulfotransfer_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36978:SF4">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36978">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOTIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17784">
        <property type="entry name" value="Sulfotransfer_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="254" mass="29537" checksum="64892D5636AC9147" modified="2022-01-19" version="1">MQVLSLAMPRTGTVTMQAALEILGCKPTYHGYTPLYNIDECAKWISAFEAKYHNRGPLFTREDWDDLLGNYQAVTDSPAICFAEELIKAYPEAKVVLVERNIESWYQSFDGMIQEMYNPIIHVLRLLDPQVLRPTATMFNYVYKDRRGFCRTDNKKELQHTARTLYREHYAHVRRVCPKDRLLNFRLEDGWGPLCEFLGKEKPDVPFPRLNEGAALAEMFKEFQKRSMLLVLRNLTLVGVSVWVVLLAVGWARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBP4</accession>
    <name>A0A8H6CBP4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149823.1">
        <property type="nucleotide sequence ID" value="XM_037294442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBP4"/>
    <dbReference type="GeneID" id="59331931"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034031488" evidence="2">
        <location>
            <begin position="19"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="252" mass="26649" checksum="4761BD48DC203DFA" modified="2022-01-19" version="1">MKTSYLLVSLLASFKATAKPIGGSDFDIAPHSRVEQRAAVPAPPPPPPAPNPGPAPAPGPAKAADPPPAPTPLPPTLDGVVLDGGVVNYNPNNHNINNCWTVPVQRPPPSAPYAPGTCSVCGLQRNGTDGSADYQLWARDNLNNAMGNMSPTTVWPLDGMRSPADDSKNQSVIQMGGSPLQLWALEAEDPNYSMVYMRWVTPPHHLSAPLNDTTIPQVSFDPNPKMINTETCSSAGTALNGAVVYFICYFAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC19</accession>
    <name>A0A8H6CC19_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149929.1">
        <property type="nucleotide sequence ID" value="XM_037293851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC19"/>
    <dbReference type="GeneID" id="59331339"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="210" mass="22777" checksum="0D4E544ADCFB5341" modified="2022-01-19" version="1">MDEGWLLREKHHSGRLYDCQLGTEREKYDFESVWVSERVESWTVHRGIYLLNTAPEQHLPSARLCTPLFQHLDTASPMATLFLLVTPCIALMAPLINPAISILAVLALTVMLSVALTALFLWTWNTGDHDENEAVADDATNIVRTALAALARDPGATSASFRVVNGVVVHRDPTAGVPNGGDGPANGVRSEEVGSGSQDFSGTHGREMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC86</accession>
    <name>A0A8H6CC86_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_003091</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16638"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150093.1">
        <property type="nucleotide sequence ID" value="XM_037294015.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331503"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Chitin synthase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF13">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="753"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="789"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1047"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1438"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1473"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1499"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="13">
        <location>
            <begin position="818"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="domain" description="DEK-C" evidence="14">
        <location>
            <begin position="1718"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="344"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6220658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1776" mass="197771" checksum="8953B4E41E4ACC29" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCF5</accession>
    <name>A0A8H6CCF5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Conidiation-specific protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150387.1">
        <property type="nucleotide sequence ID" value="XM_037293558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCF5"/>
    <dbReference type="GeneID" id="59331045"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018824">
        <property type="entry name" value="Conidiation-specific_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10346">
        <property type="entry name" value="Con-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="8494" checksum="27363BB6503C3AD8" modified="2022-01-19" version="1">MADEIHDSTAAGTAKENVPTMSNPMDNIHGNVEDISNKASGYKAAISNPNTKPSTKENAKEMLEKLGGEDAFMKNNGKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCH7</accession>
    <name>A0A8H6CCH7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150154.1">
        <property type="nucleotide sequence ID" value="XM_037294076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCH7"/>
    <dbReference type="GeneID" id="59331564"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="37367" checksum="D5A03EE939C7A3D7" modified="2022-01-19" version="1">MPTFQTLPVELQMLIMSNIHLNEIENFTLCTKFVHQLCKERLMEQYARKRYFFTIAVGHIDNNTWDGDPQIRGVHPFPILRDLLADPQTWLHAETLIVGRLEEPSADGFGDQEEAELDEFGLRDVVAQFKRNTWLLCKEFEKGFIDPSKVTSLEFYQSAIDAALFSITLWSIKGLKRFVYSFWASPPLDDAQWELRQIVGYLEAYTRKTLCHLELTGSSDVQRAENLFFEKGEPCIGSLCAFEVSKRIRLETMTLYKEIEGADSCTGEKEDIIYVEPGADLLVEPERVVDTLSALTCRGGLVGGLSNEDAIAMLEDLPARDRPPKMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCV9</accession>
    <name>A0A8H6CCV9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150334.1">
        <property type="nucleotide sequence ID" value="XM_037293505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCV9"/>
    <dbReference type="GeneID" id="59330992"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="82" mass="8961" checksum="19C8994FDA3886DE" modified="2022-01-19" version="1">MSYKRTNNAAATEPPPSPPLDGGGHQALILAINNTKRIADLFGYLRRPSIPIKKMLGLRLSRSTKGKRHGNWGAEEHAKTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDK3</accession>
    <name>A0A8H6CDK3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002434</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151013.1">
        <property type="nucleotide sequence ID" value="XM_037293360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDK3"/>
    <dbReference type="GeneID" id="59330847"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034520539" evidence="1">
        <location>
            <begin position="28"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="182" mass="20313" checksum="A7030E33B04FFBF1" modified="2022-01-19" version="1">MSHQRTIQRLALSLLTIFSTFTPATSAATPPTLASISHPVLPNLPNPNSTISFDPPRIVIKIDATHDIHFTQYRHLFPERNYFAAIISLQTSLIAQYFERQGEDLDAGALSLDAYGAEIYLDNLTGRLTYAMAHRMLGELGEFLMGGYRCVARFELWETQGAQAEQLSVGWLEPEDTNKNAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDR4</accession>
    <name>A0A8H6CDR4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151027.1">
        <property type="nucleotide sequence ID" value="XM_037292488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDR4"/>
    <dbReference type="GeneID" id="59329974"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="424" mass="47216" checksum="D859F17D51339319" modified="2022-01-19" version="1">MAPAQLWFFLWEDIRSDNFKQDFTTFDQIQKEGITQAKILNRLGRTGTPEEYSFFIHSDSMKASVQILSDKAIQATWQLYCRQDSSVVVSVRKTDDPSPNSSRAPSYSIALAARRAASSGSSVQVPEIPLIAAAHQVSSGMDVVLRTPLADLPEDEKVVIEVDSGESFAFSREYVTCRFLVPACFQSLTSYTAWVNSQAQDGKDLTEIKVTTIDNCGFPTDVQLNRYVWQRSLIRGPWKQDFLSLWDQYVRLTNEREATIAQLCLNNPAFTQNCINFIRDLQYFDSEKSARDRLESTVHQFYIGKPYFDNQTVGVILQMRSVGGTLESQLQTLAAKQTGNGSVCASHDLSPVYQSYLGVSWDKDKSKATSSQYTMSKTELSSEGNKMQQLSRKMSKTLLGQFGKKKGGGKGGRSGGGSSESSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE86</accession>
    <name>A0A8H6CE86_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001751</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151218.1">
        <property type="nucleotide sequence ID" value="XM_037292679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE86"/>
    <dbReference type="GeneID" id="59330165"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025969">
        <property type="entry name" value="ABA_GPCR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022535">
        <property type="entry name" value="Golgi_pH-regulator_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015672">
        <property type="entry name" value="GPHR/GTG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 89-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948:SF0">
        <property type="entry name" value="GOLGI PH REGULATOR A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12430">
        <property type="entry name" value="ABA_GPCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12537">
        <property type="entry name" value="GPHR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="396"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Golgi pH regulator conserved" evidence="7">
        <location>
            <begin position="189"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Abscisic acid G-protein coupled receptor-like" evidence="6">
        <location>
            <begin position="320"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="527" mass="57590" checksum="E1CFE068BD4BC35D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEB3</accession>
    <name>A0A8H6CEB3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001783</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151250.1">
        <property type="nucleotide sequence ID" value="XM_037292711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEB3"/>
    <dbReference type="GeneID" id="59330197"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF68">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="636" mass="70971" checksum="F180C2E36E65189A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEI2</accession>
    <name>A0A8H6CEI2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Microtubule associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151555.1">
        <property type="nucleotide sequence ID" value="XM_037292136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEI2"/>
    <dbReference type="GeneID" id="59329622"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF41">
        <property type="entry name" value="FASCETTO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="464"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="669"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="718"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="521"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="674"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="773" mass="86801" checksum="04F1FA6181EE38FE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CES2</accession>
    <name>A0A8H6CES2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_001248</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151597.1">
        <property type="nucleotide sequence ID" value="XM_037292178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CES2"/>
    <dbReference type="GeneID" id="59329664"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017059">
        <property type="term" value="C:serine palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000004">
        <property type="entry name" value="2-amino-3-ketobutyrate coenzyme A ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000047">
        <property type="entry name" value="serine palmitoyltransferase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF3">
        <property type="entry name" value="LD36009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="17">
        <location>
            <begin position="253"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6222162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="683" mass="75697" checksum="40DFD8C4CF6BA869" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEV8</accession>
    <name>A0A8H6CEV8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151391.1">
        <property type="nucleotide sequence ID" value="XM_037292852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEV8"/>
    <dbReference type="GeneID" id="59330338"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024312">
        <property type="entry name" value="TACC_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12709">
        <property type="entry name" value="Fungal_TACC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="600"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="618"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="683" mass="74809" checksum="2E2EE8068E548727" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF07</accession>
    <name>A0A8H6CF07_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001373</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151722.1">
        <property type="nucleotide sequence ID" value="XM_037292303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF07"/>
    <dbReference type="GeneID" id="59329789"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF66">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="260" mass="29380" checksum="1E0E2ECBA3A55A0F" modified="2022-01-19" version="1">MASPTSRFSGTGALSPVSEDENVKVARRLTLVRNLRAPAFQYAWTFYHDKYTDSQNYEGRLTLLKEDIVTAKPFWEVLNSFPFDALKLKDSVHFFKRGVKPVWEDPRNVKGGSWTFRVPKAQSEDFWKEVLMLAVGEQFVDVIQPRDDLCGLSMSVRFNSNLITIWNRDGANEKTKDGILAVVLENVSPELKPQPRNYYYKRHSEHAGFSEVVARASERSKEAEEGRIAEAEVRGPEGDEALLKDVEGIDLKELGGVAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFF5</accession>
    <name>A0A8H6CFF5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_001312</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LU7TM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151661.1">
        <property type="nucleotide sequence ID" value="XM_037292242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFF5"/>
    <dbReference type="GeneID" id="59329728"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053938">
        <property type="entry name" value="PTM1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF1">
        <property type="entry name" value="GH17801P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21902">
        <property type="entry name" value="PTM1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034840505" description="Integral membrane protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="PTM1-like N-terminal" evidence="11">
        <location>
            <begin position="31"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="10">
        <location>
            <begin position="184"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="458"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="506"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6222226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="59449" checksum="017C74680797A782" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFJ4</accession>
    <name>A0A8H6CFJ4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000628</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151928.1">
        <property type="nucleotide sequence ID" value="XM_037291566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFJ4"/>
    <dbReference type="GeneID" id="59329046"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001425">
        <property type="entry name" value="Dynamin GTPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF66">
        <property type="entry name" value="INTERFERON-INDUCED GTP-BINDING PROTEIN MX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="5">
        <location>
            <begin position="64"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="4">
        <location>
            <begin position="669"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="613"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="613"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="626"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="638"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="761" mass="85131" checksum="E133DD2D5C1CD423" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFQ2</accession>
    <name>A0A8H6CFQ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hyaluronan/mRNA-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000674</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151973.1">
        <property type="nucleotide sequence ID" value="XM_037291611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFQ2"/>
    <dbReference type="GeneID" id="59329092"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039764">
        <property type="entry name" value="HABP4/SERBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006861">
        <property type="entry name" value="HABP4_PAIRBP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019084">
        <property type="entry name" value="STM1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12299:SF17">
        <property type="entry name" value="AT19571P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12299">
        <property type="entry name" value="HYALURONIC ACID-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09598">
        <property type="entry name" value="Stm1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01233">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034864875" description="Hyaluronan/mRNA-binding protein domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Hyaluronan/mRNA-binding protein" evidence="5">
        <location>
            <begin position="151"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="34"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="97"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="216"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="345"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="406" mass="42916" checksum="ED19FE1214E41084" modified="2022-01-19" version="1">MKLYMSLISFALYAMAIANIRNLQNLYELLGNDSDQDSDKEPAPPTKVTDKPVARHGKRDAPASAPSEARGGGAGGERGGRAGRARGRGGFSGNDGVFRDREAGSTNNRDKPTDDGLRQDRHANRIEGGRDRGSYDGRGGRGGRAGRGGRGNRDDRHTRGVPNDHVKQADQSWGAPTGESEWKDEQAGEAIAKAEEKDEGAAGGWNASAAPGDDWNSGAAAVSTDAAPTADADGTAPAEGTTPANAPAEPVAEPEPEDNSRSYADYLAEQAEKKLKLGGGVPEARKPNEGSKDKKYDNAKPVAKDEQEDFFSGKSEKAKRERQRKEKNVLDIDIKYVEPQTGRGGGDRGGRGRGRGDRGDFRGRGRGRGDGFKPRTDSGFRGGRGRGDGGSVNVKDENAFPSLGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFS0</accession>
    <name>A0A8H6CFS0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2'-phosphotransferase</fullName>
            <ecNumber evidence="3">2.7.1.160</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000739</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2'-phospho-[ligated tRNA] + NAD(+) = mature tRNA + ADP-alpha-D-ribose 1'',2''-cyclic phosphate + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:23324"/>
            <dbReference type="Rhea" id="RHEA-COMP:11106"/>
            <dbReference type="Rhea" id="RHEA-COMP:11107"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:76596"/>
            <dbReference type="ChEBI" id="CHEBI:82883"/>
            <dbReference type="ChEBI" id="CHEBI:85027"/>
            <dbReference type="EC" id="2.7.1.160"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.160" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152038.1">
        <property type="nucleotide sequence ID" value="XM_037291676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFS0"/>
    <dbReference type="GeneID" id="59329157"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000215">
        <property type="term" value="F:tRNA 2'-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.970">
        <property type="entry name" value="RNA 2'-phosphotransferase, Tpt1/KptA family, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002745">
        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042081">
        <property type="entry name" value="RNA_2'-PTrans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042080">
        <property type="entry name" value="RNA_2'-PTrans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684">
        <property type="entry name" value="PUTATIVE PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684:SF2">
        <property type="entry name" value="TRNA 2'-PHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01885">
        <property type="entry name" value="PTS_2-RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="265"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="300"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="328" mass="36324" checksum="83647A57CB173498" modified="2022-01-19" version="1">MSSSRKGKGASREVDISKSLSYLLRHGAKTEGIQLDEAGWANVADVLLWRRIASLKVDLAELQSVVATNDKKRYQIEPDPAGPLRAPSATASTASISDLSNWRIRATQGHSIATVSSEQIFKPILLTDPDCPEFVVHGTDNRPWKEIERSGGLKPMGRKHIHFATRLPDKMPPLNRDFQSRSKPNKEPVDKVVSGIRNTSTVVIWVDVKKSLEGGVKWWRSENGVILTEGVGEPKMLGFEWVKWVEVRGEGKILYGEKVESEEVGEMEERMDRLGVGLGDGEEGSRDKVDGAKAPQGELNGEKVLREKEANGNEKLEPAAPVKDKWDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFT5</accession>
    <name>A0A8H6CFT5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyltransferase family 17 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001522</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151871.1">
        <property type="nucleotide sequence ID" value="XM_037292452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFT5"/>
    <dbReference type="GeneID" id="59329938"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003830">
        <property type="term" value="F:beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006813">
        <property type="entry name" value="Glyco_trans_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12224:SF0">
        <property type="entry name" value="BETA-1,4-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12224">
        <property type="entry name" value="BETA-1,4-MANNOSYL-GLYCOPROTEIN BETA-1,4-N-ACETYLGLUCOSAMINYL-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04724">
        <property type="entry name" value="Glyco_transf_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="42198" checksum="28FCE20AAE03357E" modified="2022-01-19" version="1">MLKRQILGLLAALILLYALSYVARDFYWHHEDKSNVPYKGPYTALIEGDPSLGFLSLQDASTFCQRRRLDIYETRAQRRKVYDLFLINTELDWLEIRLNDLNEEVDYFVILESDITFQENPKPLHFENNYSKFEPFHSKIIHRIVNFTDSSIPAGDTWEHERFTRNALFTQALTSLSGEQAPTQGDVLVIGDVDEIPRPSTLVTLRNCAFPPRVTLRSHFYYYSFQWLHRGEQWAHPQATFFNGLKDTINPEDLRTGKPHVELYNAAWHCSSCMQTMKHLVNKIESFSHKGYNDPYILDPQRLLQRVRKGEDLFERETEVFDRVDDNMDVPRYLLQEGNRQKFAYMLDRDPPSANFQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG44</accession>
    <name>A0A8H6CG44_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_000994</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152289.1">
        <property type="nucleotide sequence ID" value="XM_037291927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG44"/>
    <dbReference type="GeneID" id="59329412"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000037">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050777">
        <property type="entry name" value="SET2_Histone-Lys_MeTrsfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22884">
        <property type="entry name" value="SET DOMAIN PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17907">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00570">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AWS" evidence="11">
        <location>
            <begin position="358"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="9">
        <location>
            <begin position="416"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="10">
        <location>
            <begin position="540"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="632"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="641"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="692"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6222943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="748" mass="83208" checksum="0B1FA3A3C28F6A64" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGG7</accession>
    <name>A0A8H6CGG7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">pH-response transcription factor pacC/RIM101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_000807</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the pacC/RIM101 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152105.1">
        <property type="nucleotide sequence ID" value="XM_037291743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGG7"/>
    <dbReference type="GeneID" id="59329225"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000993">
        <property type="entry name" value="pH-response transcription factor pacC/RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050806">
        <property type="entry name" value="pacC/RIM101"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC/RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257:SF1">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC_RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="131"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="356"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="492"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6222759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="593" mass="63945" checksum="860CB23F460368C2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGX2</accession>
    <name>A0A8H6CGX2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152307.1">
        <property type="nucleotide sequence ID" value="XM_037291945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGX2"/>
    <dbReference type="GeneID" id="59329430"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="404" mass="45333" checksum="35286A16CBC9BAF7" modified="2022-01-19" version="1">MVANVQDKHVELDKKKNSSGGSRGADPLLPAQCALLSRRPDYLTHPVTYNATFIPHAAFQPPEPLPHRYSDTIDTFSSYKYRNTCNISSLDLHAPFAPLCPDRESMVTAMSSGGRIGNDAPYMPRGCDMRWFSTEEICEILARFEKVIIIGDSMMRHVIGSINVMIREDLGYGAVTDWNFSPEERKDCFCNYQFNVKACSIQGIFKTADVVRNDPDSIACPVNSIDVMIEQMIRFPIASEELDRLRSLLPATKPSRPYAFIFGHGLWNDLDLQATLDWLDTILDTVLSQAPWVSPSSSLSSSGSSTSHGRKRETGFWPRLFLTPNAPGKDKPDEWIITQGNKATMIFEESVGIEVRKRGLEHLGTWNATVQSNKFDGVHVDLRGNLVKAMMVMNWLSMLEVESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH44</accession>
    <name>A0A8H6CH44_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannosyl phosphorylinositol ceramide synthase SUR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000254</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152629.1">
        <property type="nucleotide sequence ID" value="XM_037291194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH44"/>
    <dbReference type="GeneID" id="59328673"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000001">
        <property type="entry name" value="MIPC synthase subunit (SurA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051706">
        <property type="entry name" value="Glycosyltransferase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385:SF20">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE CSH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="40661" checksum="36831B91EB22B5B8" modified="2022-01-19" version="1">MRRGLVIFLIINALIIVFLVRSVFTLLTLLVEDGSADAIHSAEIPPPNSPRIDDRPQLIPKIIHQTYVNDTVPERWREAQKSCVDLHEDYEYKLWTDAKSRELIATEYPWFLSTFDNYSQPIQRADAIRYFVLSHYGGIYIDLDDGCNRRLDPLLSYPAWVRRTNPTGISNDAMGSVPHHPFFTLVIESLIPYNRNWKLPYITVMYSTGPLFLSVIWQEYKRGRPSSTIAGSSVLGGGSARGWDGSAVGRVRILMGDEYNKQPWAFFRWHGGSSWHGKDARLIFWMGKHWMMLTASGFLVAGFVGLGLWWIYGRVLLLGERRRKGGKGAPSAWAQRIPLWKTASATAYELTDRHEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH87</accession>
    <name>A0A8H6CH87_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_000315</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152689.1">
        <property type="nucleotide sequence ID" value="XM_037291254.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59328734"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15480">
        <property type="entry name" value="fMyo2p_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01380">
        <property type="entry name" value="MYSc_Myo5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046943">
        <property type="entry name" value="Fungal_Myo2/2A_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036103">
        <property type="entry name" value="MYSc_Myo5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50084">
        <property type="entry name" value="Myosin S1 fragment, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="14">
        <location>
            <begin position="6"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="13">
        <location>
            <begin position="74"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="12">
        <location>
            <begin position="1228"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="656"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1104"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1560"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="923"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1113"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1560"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="168"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6223472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1598" mass="181468" checksum="6A44E35B5E394A1A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIT6</accession>
    <name>A0A8H6CIT6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010869</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153354.1">
        <property type="nucleotide sequence ID" value="XM_037301720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIT6"/>
    <dbReference type="GeneID" id="59339259"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05120">
        <property type="entry name" value="APH_ChoK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF48">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="40823" checksum="4C6B993B9981B166" modified="2022-01-19" version="1">MAENPSVKDATATFSFIKSARAPPNVILPHSESVHAQSLLSSMELSIALPFIAADVPSALPTNAEIEASKDVIAVTGGRRVVRVGTHFVVKYGLGITILEGENMLFVRSTTTIPIPKVYALYTETNTKKNYIVMENIEGHTLAYKWPSLTESQKRAIATKLKLCFDELRQLPSPGYFGSLGKRPLLDDIFWTSQKKSSINGPFENEDAVNEAMAQKYTFEVYGRTNYVADFYRQALPHILCGHAPTFTHADFQRKNFIVQAVPPKVGLWNDCQHQPNRIRDAPPRDGLWTAKRPVEEEFKVTIIDWEKAGWYPSYWEYGTALYAVGRWDDDWGLFLRDMLSPYYSEARWLQTLRFELWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ57</accession>
    <name>A0A8H6CJ57_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="6">1.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010989</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153472.1">
        <property type="nucleotide sequence ID" value="XM_037301838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ57"/>
    <dbReference type="GeneID" id="59339379"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000014">
        <property type="entry name" value="D-lactate dehydrogenase (Cytochrome), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF116">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE (CYTOCHROME) (AFU_ORTHOLOGUE AFUA_7G02560)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="8">
        <location>
            <begin position="142"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="571" mass="62240" checksum="033F2EAD00852D8D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKA7</accession>
    <name>A0A8H6CKA7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153890.1">
        <property type="nucleotide sequence ID" value="XM_037301073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKA7"/>
    <dbReference type="GeneID" id="59338610"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="532" mass="58093" checksum="28AFB8EF50DA2C4F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLD5</accession>
    <name>A0A8H6CLD5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphoribosylformylglycinamidine synthase</fullName>
            <ecNumber evidence="4">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Formylglycinamide ribonucleotide amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Formylglycinamide ribotide amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HO133_009520</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154229.1">
        <property type="nucleotide sequence ID" value="XM_037300381.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337915"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000005">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000008">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000005">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000024">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.750">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00419">
        <property type="entry name" value="PurL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040707">
        <property type="entry name" value="FGAR-AT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055181">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010073">
        <property type="entry name" value="PurL_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01735">
        <property type="entry name" value="FGAM_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003672">
        <property type="entry name" value="PRK05297.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18076">
        <property type="entry name" value="FGAR-AT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22689">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109736">
        <property type="entry name" value="FGAM synthase PurL, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase N-terminal" evidence="20">
        <location>
            <begin position="32"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="19">
        <location>
            <begin position="174"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="18">
        <location>
            <begin position="444"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="FGAR-AT PurM N-terminal-like" evidence="21">
        <location>
            <begin position="677"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="18">
        <location>
            <begin position="873"/>
            <end position="976"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF18076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF22689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF6225520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1352" mass="147374" checksum="A995B311F39FDF1B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMB0</accession>
    <name>A0A8H6CMB0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009883</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154590.1">
        <property type="nucleotide sequence ID" value="XM_037300742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMB0"/>
    <dbReference type="GeneID" id="59338278"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="114" mass="13082" checksum="3B6C44C21455085D" modified="2022-01-19" version="1">MRLTASNKASKLAELAELDSKVRSFLEMVMGELGWRQTLICVPVALCIRLLFLLHGSVLDTLQHSKSETDHQKRDMSWSGLDMERNKLVVDEALSRDIDSLLSAEEEYRKTWVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMJ1</accession>
    <name>A0A8H6CMJ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome c oxidase assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009155</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PET117 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154842.1">
        <property type="nucleotide sequence ID" value="XM_037300016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMJ1"/>
    <dbReference type="GeneID" id="59337550"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031568">
        <property type="entry name" value="Pet117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28163">
        <property type="entry name" value="PROTEIN PET117 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28163:SF1">
        <property type="entry name" value="PROTEIN PET117 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15786">
        <property type="entry name" value="PET117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034214273" description="Cytochrome c oxidase assembly protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="9395" checksum="93034B59C6E94210" modified="2022-01-19" version="1">MSRAAKLTLAGTSIGAFAVVALVHYQQNSEKAAMHAGVVRDIEQQRVKKERQADFEMQKALEEEYRKVQQVHDGEGGPEKARGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN91</accession>
    <name>A0A8H6CN91_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7492 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009197</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154884.1">
        <property type="nucleotide sequence ID" value="XM_037300058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN91"/>
    <dbReference type="GeneID" id="59337592"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055915">
        <property type="entry name" value="DUF7492"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24320">
        <property type="entry name" value="DUF7492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034033710" description="DUF7492 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7492" evidence="3">
        <location>
            <begin position="20"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="489"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="580" mass="58821" checksum="ADAB73D799A90CB2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNB6</accession>
    <name>A0A8H6CNB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GPI-anchored wall transfer protein</fullName>
            <ecNumber evidence="9">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009421</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Probable acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Endoplasmic reticulum membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the PIGW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.-.-" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155108.1">
        <property type="nucleotide sequence ID" value="XM_037300282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNB6"/>
    <dbReference type="GeneID" id="59337816"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6226555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="496" mass="54845" checksum="C68DFE3573376C49" modified="2022-01-19" version="1">MAQSYKTLKENFVSNLSGGTVSEINWVTAVAPAAYLLWAALQSRQGYFLEYGPPAFLTDLLLNVGGILLALTMYSSNAILLNTLLVSPAVLLCVLPSTTNYRKRASKPLANKPAESTGAKEELEPLPIRPFITIYRGGMIVVTCLAILAVDFRIFPRRFAKVETWGTSLMDVGVGSFVFSAGLVSARPVLQARFARMKCSFRSQLQSAFRASLPLLVLGFIRLYSVKGLDYAEHVTEYGVHWNFFFTLAFLPPFVAILQSVSALVPYTGLVSILISGLYQTLLECTRLKAFILTAPRTDFISQNREGVFSFWGCVAIFLAGQALGFDVLPRYPSQTSATDSDWQQRKRLLKRLAVWSAVWVSLFYSVTSYNIGFAFQVSRRLANQPYVFWVSAFNCAQITVFCLVETAFFPGVYKAATRTLERRECEKATSRVLRAFNRNGLAIFLLANLLTGLVNLTLDTLSMSRIGAMVVLVGYAALLTAVAVGLDAWNLSIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNG2</accession>
    <name>A0A8H6CNG2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal RNA-processing protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009187</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core complex (Exo-9) which may associate with the catalytic subunits RRP6 and DIS3 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric base ring of RNase PH domain-containing subunits and a cap ring consisting of CSL4, RRP4 and RRP40.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154874.1">
        <property type="nucleotide sequence ID" value="XM_037300048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNG2"/>
    <dbReference type="GeneID" id="59337582"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11370">
        <property type="entry name" value="RNase_PH_RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000004">
        <property type="entry name" value="Exosome complex component Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="8">
        <location>
            <begin position="22"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6226321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="260" mass="27609" checksum="2E037184218E593F" modified="2022-01-19" version="1">MPLDTSTYHLALLRLDGRRWNELRRLHAQISTQASADGSSYLEMGNTKVICTIAGPAEVRGAARREGAERDNAVVTVEIGIAGFSGVDRRRGGGGGGKGRGDKRTTEMQNTIASAFQSTLFTHLYPHSTITISLHILSQDGSLLAACLNAATLALIDAGVPMSDYIVACTSGLTPPKSGTESADPLLDLNGMEEQELPWLTVGTLGAGERVSVLVLESRVQVGRLEGMLAVGVDGCKQVREILDGVVRRQGKRVLESVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNV5</accession>
    <name>A0A8H6CNV5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155260.1">
        <property type="nucleotide sequence ID" value="XM_037299259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNV5"/>
    <dbReference type="GeneID" id="59336789"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034954350" evidence="2">
        <location>
            <begin position="20"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="386" mass="39044" checksum="9C26A08502F03D5A" modified="2022-01-19" version="1">MTRLLPLTVALLFAGAGQAAPAPQVASTLFADPSTTPSASLSRSLGPSGPNATSSLTCYPFQDPDAGPAPPGCMCDGLDGIFPYLSSSTGQSSFNPCGFTTMPTAASATAAPFITTESNGDVVFCASSTYYNYAINTNPICAGATSVVFIVPSIASLYSKSTASFASVASVSAASVSSAISSASYVSAAAVPSAGCRDLGDDLFGETLFEVYGINGWAGSDGGRLLAEEWGCGFVSFFDFHTDTQDEFEGHLRDTQYALFFLSLFKAGCVERAVHSAGGPSPGTGPAKVQCYHTPDRRDTLLDALGSIGTARLEAVKAVVDGKSANNSLSANNEEKAVLDTSSSSPAAAGGPQPGASLGASASAALSHLLAAQRRPSTVPTATPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP98</accession>
    <name>A0A8H6CP98_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008784</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. TCR/Tet family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155648.1">
        <property type="nucleotide sequence ID" value="XM_037299647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP98"/>
    <dbReference type="GeneID" id="59337179"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000489">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000196">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000012">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF201">
        <property type="entry name" value="MFS AFLATOXIN EFFLUX PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="120"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="58"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6227340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="618" mass="66047" checksum="E6F309218907BB18" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPI4</accession>
    <name>A0A8H6CPI4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Increased loss of mitochondrial DNA protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008694</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155560.1">
        <property type="nucleotide sequence ID" value="XM_037299559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPI4"/>
    <dbReference type="GeneID" id="59337089"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018815">
        <property type="entry name" value="Incr_loss_mito_DNA_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28029">
        <property type="entry name" value="PROTEIN ILM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28029:SF1">
        <property type="entry name" value="PROTEIN ILM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10311">
        <property type="entry name" value="Ilm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034679096" description="Increased loss of mitochondrial DNA protein 1" evidence="2">
        <location>
            <begin position="22"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="19166" checksum="8F006BBB4AE02630" modified="2022-01-19" version="1">MAILSAQSLLRTLSLFHLTLSYYLFTSPSTIASQNLVYVLGAAMDIPDPPISLSTPSPASTLAALFLALFAISDLTSSGLPEEVGSHYWSSQAPIRLTFFFAVTGYSYAGKPGGLWGGKGRRGGEVLCNSVVFTWGFLEMLWWFWIYVTLRDEKRELMVKVQERRKAEEDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPK7</accession>
    <name>A0A8H6CPK7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008725</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 14-3-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155590.1">
        <property type="nucleotide sequence ID" value="XM_037299589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPK7"/>
    <dbReference type="GeneID" id="59337120"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000002">
        <property type="entry name" value="14-3-3 protein epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="4">
        <location>
            <begin position="5"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="231"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6227282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="274" mass="30514" checksum="15EEC7D077DB61B7" modified="2022-01-19" version="1">MTSERESKTFLARLCEQAERYDEMVTYMKIGGELTVDERNLLSVAYKNVVGTRRASWRIISSIEQKEESKGSDKHVGTIRDYRQKIETELERVCQDVLDVLDESLIPKAESGESKVFYHKMKGDYHRYLAEFASGEKRKVAATAAHEAYKNATDVAQTELTPTHPIRLGLALNFSVFYYEILNSPDRACHLAKQAFDDAIAELDSLSEESYRDSTLIMQLLRDNLTLWTSSDGGDAEAAPSGEASKEGEKAAEPKAETSAASEAPAEAARPSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPV3</accession>
    <name>A0A8H6CPV3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RhoGAP-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008732</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155597.1">
        <property type="nucleotide sequence ID" value="XM_037299596.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59337127"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000452">
        <property type="entry name" value="Rho GTPase activator (Bem3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF129">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 16F, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="919"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="1208"/>
            <end position="1412"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="464"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="969"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1102"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1495"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
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        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
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        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
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        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
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        <location>
            <begin position="496"/>
            <end position="513"/>
        </location>
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        <location>
            <begin position="514"/>
            <end position="525"/>
        </location>
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        <location>
            <begin position="535"/>
            <end position="562"/>
        </location>
    </feature>
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        <location>
            <begin position="592"/>
            <end position="606"/>
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        <location>
            <begin position="717"/>
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        </location>
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            <begin position="1108"/>
            <end position="1121"/>
        </location>
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        <location>
            <begin position="1122"/>
            <end position="1136"/>
        </location>
    </feature>
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        <location>
            <begin position="1533"/>
            <end position="1542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6227289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1542" mass="167674" checksum="35030CE11A81DC39" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS98</accession>
    <name>A0A8H6CS98_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Retrograde regulation protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156250.1">
        <property type="nucleotide sequence ID" value="XM_037298914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS98"/>
    <dbReference type="GeneID" id="59336443"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
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    </dbReference>
    <dbReference type="InterPro" id="IPR057512">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="38"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="RTG2 C-terminal" evidence="2">
        <location>
            <begin position="338"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="58304" checksum="39B3CFDA43A5A976" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6U7</accession>
    <name>A0A8H6F6U7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Heme haloperoxidase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_006774</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the chloroperoxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147107.1">
        <property type="nucleotide sequence ID" value="XM_037297671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6U7"/>
    <dbReference type="GeneID" id="59335175"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.489.10">
        <property type="entry name" value="Chloroperoxidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000028">
        <property type="entry name" value="Chloroperoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036851">
        <property type="entry name" value="Chloroperoxidase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33577:SF15">
        <property type="entry name" value="HEME HALOPEROXIDASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33577">
        <property type="entry name" value="STERIGMATOCYSTIN BIOSYNTHESIS PEROXIDASE STCC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01328">
        <property type="entry name" value="Peroxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47571">
        <property type="entry name" value="Cloroperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51405">
        <property type="entry name" value="HEME_HALOPEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034730406" description="Heme haloperoxidase family profile domain-containing protein" evidence="8">
        <location>
            <begin position="18"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Heme haloperoxidase family profile" evidence="9">
        <location>
            <begin position="85"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6217672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="428" mass="45325" checksum="BC8D12496DA4336F" modified="2022-01-19" version="1">MSGLLLLAIMVVPNVIAFPWVAGMSGVRSISEIEAQLAKRQSTCPFNANHVPAAPATSKFPYAGAINGLPSTRPGNFQVPANGDTAHAFVAPGPNDIRGPCPGLNTAANHNFISHDGVTTFTELLNAQQNIYNVGYDLAVLLAVLGVGLDGDPITEKMSIGCDATSRTSLTGSLLGAEGGLDSHNKFEGDASLTRADFFFGDDHTFNETYFAELTALAYKGGDKTDVINSYSFNQTVVAQHQFNRYQNSKATNPNFFFGPKAILLYGAASFLYELFPSHGPDGAPAFLDLGSFYGTTNPNANGNVVFNNTEHVPINPTGGPGWFSRTNPYNISALVLQVVDLYTAHPVLFGGNVGVGNFDALNFGAISNGQLSLNPQDVLCLLYQLATDDVPDSLSSLLTLPSEILNFTTSKLNPIFANSGCTLASPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7Q8</accession>
    <name>A0A8H6F7Q8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Guanine nucleotide exchange factor Vps9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006645</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147253.1">
        <property type="nucleotide sequence ID" value="XM_037297543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7Q8"/>
    <dbReference type="GeneID" id="59335046"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14369">
        <property type="entry name" value="CUE_VPS9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041545">
        <property type="entry name" value="DUF5601"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041804">
        <property type="entry name" value="Vps9_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18151">
        <property type="entry name" value="DUF5601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="3">
        <location>
            <begin position="414"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="748"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="791" mass="87350" checksum="A926AAB3B162E0B1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7V7</accession>
    <name>A0A8H6F7V7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006187</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147661.1">
        <property type="nucleotide sequence ID" value="XM_037297087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7V7"/>
    <dbReference type="GeneID" id="59334589"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051104">
        <property type="entry name" value="FAD_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720:SF3">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01460)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="20"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="431" mass="46433" checksum="45A74E729A258EBD" modified="2022-01-19" version="1">MASTTNAPTTESSKPKAFSIAIAGGGISGLTLALGLLKYEHIDVQIYEAAPAFGEIGAGVAIGPNSQRALKLIGPHARDAFDKHATSNMWEAYADVFTEHIVGAGEHEGEAFASQKTQGGMRSVHRAQFLDELVKGVPAERAHFNKRLQSTEEKEGGGVLLHFKDGTTATADAVVGADGIHSTIREYLLGERDMAAHSVFAGSVAYRGLIPMDKAVEKLGAEYAQNSMMLCGPGKAILSYPTDLGKIMNAVVIDFERPDWDSDKWIMPATSEQLNRLLSGWGKKAHGLIELLDSPSLTAWAMRDDLPASTYAIGPVAMMGDAAHASTPFQGQGAGQAIEDALVLETLLGRVHDPKHIPNAFAAYDQVRRPRSQRVVKTSREAGELFGMMAKGVGSDLKKIAEQADTRMHWIWNRDLEKQNGEAIALFEESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F808</accession>
    <name>A0A8H6F808_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147568.1">
        <property type="nucleotide sequence ID" value="XM_037296994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F808"/>
    <dbReference type="GeneID" id="59334496"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034168895" evidence="1">
        <location>
            <begin position="26"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="227" mass="24308" checksum="9B7C0B34C6E0684A" modified="2022-01-19" version="1">MPSPRKPSATAILFLLLHSASFASSQTTVSPNYASESPSIYQEYNFTYPTYATDQTILASYKDSIDVSWTSIAPHHPPSLSIECWIRNATTSPIWLPMADASRRPDYGSNLTSFALANESSYLLELATFQEYSPCQLILQDFDIFNASNGSSFTKNNNNPVFSNIFAISVQNHSAGVTWSAKSPAPEQTGTDGIGGGTSKSVAGNIRAGVSMAYAGLVGAIVVGLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F833</accession>
    <name>A0A8H6F833_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-L-isoaspartate(D-aspartate) O-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006026</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148110.1">
        <property type="nucleotide sequence ID" value="XM_037296929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F833"/>
    <dbReference type="GeneID" id="59334431"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="223" mass="23722" checksum="DE384546742770D1" modified="2022-01-19" version="1">MAWRSTGSTNAALIKNLTSNGLITSERVRNAMLAVDRAHYAPATPYHDSPQTIGFGATISAPHMHASAAESLLPYLRPDARVLDIGSGSGYLTAVLANLVGPSGTVVGVDHIQGLVDLAHRNMSKGKEGREMLEAGKVRFVKADGRLGLDGGEGGWDAIHVGAAAKEAHEVLVEQLRAPGRLFIPVGEGMQYIWVIDKKEDGSVVREKSFGVMYVPLTDAPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F860</accession>
    <name>A0A8H6F860_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148262.1">
        <property type="nucleotide sequence ID" value="XM_037296280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F860"/>
    <dbReference type="GeneID" id="59333776"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000018">
        <property type="entry name" value="RNase III domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040030">
        <property type="entry name" value="Ribosomal_mL57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28160">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L15, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28160:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="29"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="195" mass="21771" checksum="660958DA90348B97" modified="2022-01-19" version="1">MMAPIRSKPPVANNDFAVNEDPERLDNVYNRVLGKEGYKMLTDEVKWLAVTHKSFDHGRRGFNERLAFLGKRIVQLQTSLTLFHSSSATPFPETQDQYGREPFRHPALDGLAGVSAESKEQILRTGRTAQLAEKYGLNTVVRWKPRTMANLKGSGQLIVTSRALYAIVGAVALQKGGEVANNVVRERILWPLGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F863</accession>
    <name>A0A8H6F863_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147659.1">
        <property type="nucleotide sequence ID" value="XM_037297085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F863"/>
    <dbReference type="GeneID" id="59334587"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF15">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="2">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="554" mass="62381" checksum="B0C9B93465F6C6C4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8T7</accession>
    <name>A0A8H6F8T7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ceramidase</fullName>
            <ecNumber evidence="1">3.5.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148408.1">
        <property type="nucleotide sequence ID" value="XM_037296426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8T7"/>
    <dbReference type="GeneID" id="59333923"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.60.10">
        <property type="entry name" value="Penicillin V Acylase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029130">
        <property type="entry name" value="Acid_ceramidase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28583">
        <property type="entry name" value="ACID AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28583:SF1">
        <property type="entry name" value="ACID CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15508">
        <property type="entry name" value="NAAA-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acid ceramidase N-terminal" evidence="2">
        <location>
            <begin position="34"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="413" mass="46501" checksum="85F8FE8B43A59222" modified="2022-01-19" version="1">MESAGLRGRNASILKLNNQPIEDFGSDASEDGDEPPRYTIDLSLPPRKRYQHIAADFKPQIATLPILFDEIVKDLRANISVERVRWLARLLLRRVYNKEENEELRGIQETTGVEMYLLVAFNVLLDLFMGCTSGAVRVKDDDKNTKMLHFRTLDWGMDALRKVIVHLDFVEKPGGEVIASSITYIGYVGVLTGVRKGLSVSLNFRPSHDCSGRFSNFRFYSHHLLVLLGFQPSISSLLRGYLLPTLPSDTSSIDILALESIKRDLPSVPTTAAYLIFSDGENTLTIEKDHHTAVIRSATDFIVATNHDAAEENTNQSLKATHKDSFETLLDGIVVESIDRKKTAVNLWETALKRAKRNSSKKVSTHEQGVTKDSIIRWLSSSPILNEETHFATVMDPKDGKVVWTKRYIEPFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA96</accession>
    <name>A0A8H6FA96_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149923.1">
        <property type="nucleotide sequence ID" value="XM_037293845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA96"/>
    <dbReference type="GeneID" id="59331333"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="182" mass="20434" checksum="EAB5CB0C786A061A" modified="2022-01-19" version="1">MTRTRAGRDFHGLTEANYREFELAYYKYHPQSRSVSFERYLDNGTHVREIEEAVVNNFHGNRSGQCCGYGARGHGHRSHRFGSADRRGSRVPLSTSRCGPADDFDRVQFRDVPCDGRRGAGGSLDRAGHPLPRGHCRVPSPPPSRCGGESSRPRQHPQSQWNRHPASDGYSGEHWGCYGPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAJ4</accession>
    <name>A0A8H6FAJ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150223.1">
        <property type="nucleotide sequence ID" value="XM_037293394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAJ4"/>
    <dbReference type="GeneID" id="59330881"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF105">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="2">
        <location>
            <begin position="106"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="323" mass="34955" checksum="38D86616793CC2E0" modified="2022-01-19" version="1">MSQPHPRSEHPPNTSFDPPSPCTHNNNNNHNHAHVPSTAFSGDMAQTYSQRTGGCNIVLADHLISLITPSLPPPTTTPSLRILDNACGPMVLTTQCLLDPSIATYPSVHISAVDLSADFIAANRTAISSRSTLGPKVDTAVMDGTDLRFADDTFDVSFTSLGIFAFPDPVRGARELYRTLKPGGVTAATTWKGVGWLPLLHAVEEEVLQPGQAKTRLPFLEPWAVAGKLEATLREGGFERVEEGEVEGVAWWGGVGEAAYWLTATVRMMVGRGWSEEEKDKMEEGFRTVLERGVREGSELVVTEAEGGRVGCRMVAFTGLGWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAW1</accession>
    <name>A0A8H6FAW1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001705</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151172.1">
        <property type="nucleotide sequence ID" value="XM_037292633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAW1"/>
    <dbReference type="GeneID" id="59330119"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001025">
        <property type="entry name" value="ATPase family, AAA domain-containing 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF27">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="240"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="513"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="740" mass="82243" checksum="0904E2CC432306E8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAX4</accession>
    <name>A0A8H6FAX4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150509.1">
        <property type="nucleotide sequence ID" value="XM_037293680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAX4"/>
    <dbReference type="GeneID" id="59331167"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="217" mass="25159" checksum="53B4992830398DD3" modified="2022-01-19" version="1">MEARARTCMGPFISIGTGIPPIDFFSKKKGNFNNAVTTLQAALRLPSRSVGVHRKRERLSMHDNKERFSYFRFDGGERDGEIALDKWKGHQFTRLTGKDKNPGCMTLEKMYVATAAYLAEPKVQQDLTECARILVRRRQLRMRNGSEWDRYASFSYNDCNVEGCARQRSNKAQDFREHLRKFHQKIADHEMERRVLECRRVHGFYRPNPPDATPSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAY0</accession>
    <name>A0A8H6FAY0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED19</name>
        <name evidence="11" type="ORF">HO133_002770</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAY0"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013942">
        <property type="entry name" value="Mediator_Med19_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08633">
        <property type="entry name" value="Rox3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="57"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="245"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="348" mass="37490" checksum="8E76D6269B46F8FD" modified="2022-01-19" version="1">MNQLNTSQTSILTHPLLTSNHGKKISAIAMDVDDAGQRIADRIQDQDIMDTTLDNVQEASSNVADPEAPEKGQAAAGAETTPVPENSTEKDDETQQSSKQKPPIPQTLPLTQNLISLFGLQPLAATVARSDPVTGEKINKMRKSYEGQLKTLGLAGRNRSVKHQEGKLMGLREMTLWPEEEWQNQKVGGKDIHKGLPSGILAKLDKAMQMQPGPLPNNNEWENLLGNDNPKPVEATKASKIPNKPLAKANGQVNGNTANAETVRPQRTNKKRSYGDASFEGYTGYAEGIEDDDADTGGGYSSGDTQFSRKSGRAAKKRKKGYGNNPTGFGDRSGSFSSGMVGVGAYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBN1</accession>
    <name>A0A8H6FBN1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151363.1">
        <property type="nucleotide sequence ID" value="XM_037292824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBN1"/>
    <dbReference type="GeneID" id="59330310"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="370"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="462" mass="52002" checksum="9889E1331D92AFAB" modified="2022-01-19" version="1">MQQNLSAMQNIISQRLLLAKEYGTSLAIEGAFRKAEWERDRARRMGQTRGAGSTGASTYATKNSPYIDWSPIKVAAKPPADMLPSTTATRPRSRTTIPVMGPTSMTESPLIVHSSLYHPSSIGNMHAAFANQDSPPAAFDKVALTIQHRVPNPVTGTSDPYHATVSVPSSVSLNELKQAVWAGMQNDSTGSAPQALALNGWIESLVVHWDVDKQVYHHFPPTTELRDESLETMLVCLRDVKGYDWVEVGFETPCGMRTHRSSASHQCYRLIKSVAFTIELIPRIVNMKQPMVEMEGSGYTMTPPPDDLAERGTLDKHGKRRSLLREIGRQNRPRSRYTLIYLWKDMEDAKRRTSRKLAYPANPPTLKMRNPQNKNNTIQNKTKQHWQQRYPRTGAQSTHQLDQETPPRRPHREAHGHPSAWPAAAMSGNLGATNTKSRKYIADPTDRKDRRTHLTRHPNRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC29</accession>
    <name>A0A8H6FC29_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001405</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151754.1">
        <property type="nucleotide sequence ID" value="XM_037292335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC29"/>
    <dbReference type="GeneID" id="59329821"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070646">
        <property type="term" value="P:protein modification by small protein removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF7">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="7">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="141"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="6">
        <location>
            <begin position="293"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="150"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="582" mass="63791" checksum="E3D2FA418FD5EB7C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCC3</accession>
    <name>A0A8H6FCC3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152237.1">
        <property type="nucleotide sequence ID" value="XM_037291875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCC3"/>
    <dbReference type="GeneID" id="59329360"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="399" mass="44692" checksum="F4315DB36CBE8FF0" modified="2022-01-19" version="1">MDNQKLVAICRHDLRLEEDYDIARLIREVKIVQEDSSDTYARLTRKHRIKAREILSGYMLAWEYVEDLDGSGYGQRFWLCVFSESISPLLNQTLNLETRLGSVHSLEGKLSRPDALIWPEDKKKWCAMTSFQLNILIYARILAGVEKMFNDLQDYAIIDRQKRKSRPTPEIDKKADAGNDPPPPGAQSTFGPPTKHGSRRDKQPKTDVTLVPYNVAPIAQDAKPGERAASQKEEPKKAASEESYDVDPNTRPEGAVTDESGEETPSRKRALTPPYIPEKGLHQTWPAFGFKIVSKEGARALRNRAVKIDDSEESTSKTTPMTPRRSKGSRVKANRKQDSAEKNSSTVSAERSEEEHIDIEEPKKTGKGRLPSLPNSLLTRRQAASAKAAGNGSPRSEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCQ9</accession>
    <name>A0A8H6FCQ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="16">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_000378</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Protein phosphatase that specifically binds to and dephosphorylates the molecular chaperone Hsp90. Dephosphorylation positively regulates the Hsp90 chaperone machinery.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="16"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152752.1">
        <property type="nucleotide sequence ID" value="XM_037291317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCQ9"/>
    <dbReference type="GeneID" id="59328797"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07417">
        <property type="entry name" value="MPP_PP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000039">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041753">
        <property type="entry name" value="PP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013235">
        <property type="entry name" value="PPP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08321">
        <property type="entry name" value="PPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033096">
        <property type="entry name" value="PPPtase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="17">
        <location>
            <begin position="277"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR033096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6223535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="476" mass="54288" checksum="80A5F94D61A698BD" modified="2022-01-19" version="1">MAPKEESAELKAKGNKYFASHEWLNAIDSYTKAIEMYDQDPSYYCNRAQANIKLEQYGYAITDATKAIELDSGYAKAYYRRAVANTAILNSRAAVKDFKAVVRLEPSNKDAKLKLAECEKMVKRVEFLRAIEVGDPPSAFEGLDIDSMVVDPSYDGAKLGKEMTQEFIDDTISRFKNGKKLHRKYVYQIIAAVRKIVYDEPTMVEMEVHKGHQLTVCGDTHGQFFDLMELFRLNGFPTESHYYLFNGDFVDRGSWSTEIALLLYAYKWLHPKAFFLNRGNHETDDMNRVYGFEGECKAKYSERVFKLFSESFSALPLATLIGKKFFTLHGGLFSDDKISLDDVRKLNRHQQRQPGQQGLMMEMLWTDPQPMPGRGPSKRGVGLQFGPDVTKRFCEANGLEAVIRSHEVRMEGYEVEHDGHCITVFSAPKYCDSTENKGAYINIGPEHKLEFHKFDAVPHPDIKPMAYAQSSLMSMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD58</accession>
    <name>A0A8H6FD58_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Halotolerance protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000346</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152720.1">
        <property type="nucleotide sequence ID" value="XM_037291285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD58"/>
    <dbReference type="GeneID" id="59328765"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13994">
        <property type="entry name" value="STKc_HAL4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000183">
        <property type="entry name" value="Serine/threonine-protein kinase hal4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF558">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="234"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="67"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6223503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="521" mass="58524" checksum="600C45B98FFE0871" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDZ8</accession>
    <name>A0A8H6FDZ8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010064</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153737.1">
        <property type="nucleotide sequence ID" value="XM_037300920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDZ8"/>
    <dbReference type="GeneID" id="59338456"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="114"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="135"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="277"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="458"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="702"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="313"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="716"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1107" mass="122653" checksum="DA54A827A401A97D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEH7</accession>
    <name>A0A8H6FEH7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">mRNA-capping enzyme subunit alpha</fullName>
            <ecNumber evidence="3 16">2.7.7.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 16">GTP--RNA guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 16">mRNA guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_010107</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the eukaryotic GTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.50" evidence="3 16"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153780.1">
        <property type="nucleotide sequence ID" value="XM_037300963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEH7"/>
    <dbReference type="GeneID" id="59338499"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07895">
        <property type="entry name" value="Adenylation_mRNA_capping"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000011">
        <property type="entry name" value="mRNA-capping enzyme subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001339">
        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017075">
        <property type="entry name" value="mRNA_cap_enzyme_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013846">
        <property type="entry name" value="mRNA_cap_enzyme_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03919">
        <property type="entry name" value="mRNA_cap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036959">
        <property type="entry name" value="mRNA_cap_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 16" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="5 16" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="mRNA capping enzyme adenylation" evidence="18">
        <location>
            <begin position="40"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA capping enzyme C-terminal" evidence="19">
        <location>
            <begin position="241"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="active site" description="N6-GMP-lysine intermediate" evidence="17">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR036959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR036959-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6224913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="386" mass="44542" checksum="3639FBEE88918DB6" modified="2022-01-19" version="1">MGGSVPDIPGVKAEGPLLTTLRAEVAELLDRKSPNFPGAQPVSFAARHIAELQKQDYFVCEKSDGIRCLMYLTYDDQPEGRVQVTYLIDRKNDYWYVPGPIFPKSVDKPYDFHVATVLDGELVNDTQVDGSIRMKYLVFDCLVLDGNRLIHRTLDKRLGYFYEHLDNPLQAFYKKWNDDAAALPFLVERKKMELAYGIEKMFKEILPNLPHGNDGLIFTCKSTPYQFGTDQHILKWKSEEENSIDFRMVLGFPIIQPDSDDDDDDDADEGPYPDYSALPEIELYVNGDGDTDIPFGRMHVDNPEWEDMKSLQQPLNWRIVECSQDKSNQWRFLRFRDDKKEANHISTVNSVMESIQDNVSKEHLIGVAKSIRDHWKKREQPAVVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FER1</accession>
    <name>A0A8H6FER1_9LECA</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="2 3">
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        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="85" mass="9337" checksum="AB21EA03A9431195" modified="2022-01-19" version="1">MEFTKGHIVVMARPKKFVNYGTKKQTCQVLVPADAENTILQRIRVMDSWPAQSEVGQESDDGELSSSEESERASVDSGKASLIGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFA3</accession>
    <name>A0A8H6FFA3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
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        <property type="molecule type" value="Genomic_DNA"/>
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            <end position="151"/>
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            <end position="304"/>
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            <end position="111"/>
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            <begin position="171"/>
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        </location>
    </feature>
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            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
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            <end position="256"/>
        </location>
    </feature>
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        <location>
            <begin position="257"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="304" mass="33476" checksum="5E6835CC36712CB5" modified="2022-01-19" version="1">MSQLSFTFTTSSKVKTAHLVGTWDGYKGQLPLTSQGSGKWKGTFRFSEKTLKPGSRYWYYYIEDGFKPTFDKSARSQVEPSTGRTLNILDVSKSSSASLSSSAAPSSTLSKRGSTHAPHTGRALSPSKIMHPKPTKPYESRRVREADYSASPGIDDLAEHLEKASLYKMRNVSPPSSVGSSLSSRSSDRSSPSSLSSLSNSSSSDSQCRCQRYGLTRGGGRVKIDCGGKRCGYSDDSSSACSSIDSESESESEEEYEERRPRRQEVKPAAYHESKPRYVEAKPRYQTIEPRKYATTGMSRGSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFF1</accession>
    <name>A0A8H6FFF1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
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            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
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            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154699.1">
        <property type="nucleotide sequence ID" value="XM_037299873.1"/>
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    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="104" mass="11857" checksum="0418363E191F3214" modified="2022-01-19" version="1">MSASEKKHRIKHESRSQPQDGHVGAKEFEHAIEGQRSGNATEASPNKCLWKDCRLSPPMERYLDEPGPLYCKIHQQKDRYKDPENTEAKTCNATETLEDTLESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGJ4</accession>
    <name>A0A8H6FGJ4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="4 5">
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155352.1">
        <property type="nucleotide sequence ID" value="XM_037299351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGJ4"/>
    <dbReference type="GeneID" id="59336881"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF2">
        <property type="entry name" value="NEGATIVE REGULATOR OF SPORULATION MDS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="160"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="791"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="838"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="956"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="791"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="811"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="838"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1003"/>
            <end position="1017"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1092" mass="116651" checksum="31A6C9D415820C46" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHN3</accession>
    <name>A0A8H6FHN3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF803-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008231</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156435.1">
        <property type="nucleotide sequence ID" value="XM_037299099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHN3"/>
    <dbReference type="GeneID" id="59336628"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF92">
        <property type="entry name" value="SPICHTHYIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="417"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="586"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="676"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="700"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="721"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="730" mass="78952" checksum="E841AC7BCEFA76D8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHT0</accession>
    <name>A0A8H6FHT0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bactericidal permeability-increasing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156298.1">
        <property type="nucleotide sequence ID" value="XM_037298962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHT0"/>
    <dbReference type="GeneID" id="59336491"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027842">
        <property type="entry name" value="HAM1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045967">
        <property type="entry name" value="HAM1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31138">
        <property type="entry name" value="CHROMOSOME 19, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31138:SF4">
        <property type="entry name" value="DUF5923 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14613">
        <property type="entry name" value="HAM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19343">
        <property type="entry name" value="HAM1_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAM1-like N-terminal" evidence="3">
        <location>
            <begin position="24"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="HAM1-like N-terminal" evidence="3">
        <location>
            <begin position="242"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="HAM1-like C-terminal" evidence="2">
        <location>
            <begin position="593"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6228364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="742" mass="83086" checksum="80690A038ECF1309" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIF6</accession>
    <name>A0A8H6FIF6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ras-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_007264</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156791.1">
        <property type="nucleotide sequence ID" value="XM_037298156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIF6"/>
    <dbReference type="GeneID" id="59335663"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04138">
        <property type="entry name" value="H_N_K_Ras_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000080">
        <property type="entry name" value="Ras-like GTPase Ras1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6229149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24044" checksum="E59E37A0AE66215A" modified="2022-01-19" version="1">MRSNTPPVQFLREYKLVVVGGGGVGKSCLTIQLIQSHFVDEYDPTIEDSYRKQCVIDDEVALLDVLDTAGQEEYSAMREQYMRTGEGFLLVYSITSRQSFEEILTFQQQILRVKDKDYFPIIIVGNKCDLDSEREVSRQEGEQLARQFGCAFIETSAKSRINVDNAFFNIVREIRRYNKEMSSYPAGGGGPSTSRPPQDNSDSAGCCGGKCIVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIG6</accession>
    <name>A0A8H6FIG6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007259</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenylyl-molybdopterin + molybdate = Mo-molybdopterin + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>molybdopterin + ATP + H(+) = adenylyl-molybdopterin + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:31331"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58698"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoeA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156786.1">
        <property type="nucleotide sequence ID" value="XM_037298151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIG6"/>
    <dbReference type="GeneID" id="59335658"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061598">
        <property type="term" value="F:molybdopterin adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF30">
        <property type="entry name" value="MOLYBDOPTERIN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="1" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="3">
        <location>
            <begin position="11"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="MoeA C-terminal" evidence="4">
        <location>
            <begin position="174"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="285" mass="31746" checksum="28741D74262CE48A" modified="2022-01-19" version="1">MFEAEAYWSVLRDSNGPYFQAALRHLGIDAAYLRVVEDDQGRFEEVIEKALATGSYDAFNTTGAVSVRKFDFIRQGLTDFEAKTRFRKVAIRPRHPVLFAMLSPHGQDVKNKQREGSLLLLARESPDHCWLLQFPSHATSPILPRATGRKAHRAQLNVLGSPSQRSNSSDAVRKAVRKPSHLRLFWHGIIDGTTSQVTLSKDQGSNEVRPLLSANCWVVAPKGPEQISRGTWADCYSSLPSSIGGDTPKRDSTDQNSSSEPLRSAAGPCWIFASMKWFALPQWIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIR2</accession>
    <name>A0A8H6FIR2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156854.1">
        <property type="nucleotide sequence ID" value="XM_037298219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIR2"/>
    <dbReference type="GeneID" id="59335727"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="149" mass="16754" checksum="C17C90AA922556F1" modified="2022-01-19" version="1">MTQARQVRDLRDKIYGLLSLVKNKNSLPYLPTYDIDARPLIERARLRVQGDTVLALGVVVDGIEAVGTQIECCAQETGQSYDDVEWTQMGLIHTKFRKMVDKSTAYGSSEDVTEEVRSHKVVGGRDTDYRRRPKMRAYHGGSETDSADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIX2</accession>
    <name>A0A8H6FIX2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157045.1">
        <property type="nucleotide sequence ID" value="XM_037298410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIX2"/>
    <dbReference type="GeneID" id="59335918"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="159" mass="17335" checksum="8D91D09EA1A11B07" modified="2022-01-19" version="1">MQPFAAPSGKNSQREIVDRIHQASAGMPDTFNAQLASSLNVGIQQLSPVMLGMDYNVRWDGTQEDEKQCTPATVETRSREKAFPCTRVDSVESFRDHGARPPKVNTSTAFTIRSPETHPNEECDGTEMDTAPNPTNGTLQGAQQSIVELNPGDKGFGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJT4</accession>
    <name>A0A8H6FJT4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004223</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157143.1">
        <property type="nucleotide sequence ID" value="XM_037295142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJT4"/>
    <dbReference type="GeneID" id="59332632"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000018">
        <property type="entry name" value="Putative MFS multidrug transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF59">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="428"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="56"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="553" mass="59608" checksum="ADCD9BBC54B352BD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKS8</accession>
    <name>A0A8H6FKS8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157621.1">
        <property type="nucleotide sequence ID" value="XM_037295620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKS8"/>
    <dbReference type="GeneID" id="59333112"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="27094" checksum="3EF3DA9E53FDC8CC" modified="2022-01-19" version="1">MQTTATHHSTVPSPHLPANHSRKSLISQNLSAKSNTNAIADRTVMRNAIRRVGEDTGARRRGRRAMALEATRIGAREREAVRAEGVAVDLAEVVADLPGARVRVLELQDRLLDLPRHLAHLDGAAVVGGDVGLGVGAGAGGELVDGAAGGDAVVFASATDVEVHGEGAVVAHVRLAGGAGGGVDVGDGGSAGDDGSGGHGCGLGGVGWGWAGEGTAKYPMDTSVAMTVAEKCNFWYGGQVFEVKGMWGLRSGEGEALTVEAGFMETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL36</accession>
    <name>A0A8H6FL36_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004857</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157770.1">
        <property type="nucleotide sequence ID" value="XM_037295769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL36"/>
    <dbReference type="GeneID" id="59333263"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034604234" evidence="2">
        <location>
            <begin position="18"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="254" mass="25523" checksum="004767D096D41DDC" modified="2022-01-19" version="1">MLPQLWLFTILTPLVLGVPVPRVSVIPSVRSSDRTSDIETSDGPRKMGIDHANKASWHLKGDQKGAGSKTVVVGQGSPSLGGYTAHGTGVSHSGGSVLNGRQGSSISGQASGINPPRSLSTGISGGTSGTPSGGTLGETLEESSDGSSRGTSSDSGQSEELKEGGSYEGHGEHGKHGKHGGGMKWGEMHKQVEPNGSGSTTGSASNTGSDRTTTDRKGTTTSTNTGSEDTTGTITGSESSTGSDGTTTSISKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY18</accession>
    <name>A0A8H6KY18_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Actin-related protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007040</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156570.1">
        <property type="nucleotide sequence ID" value="XM_037297935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY18"/>
    <dbReference type="GeneID" id="59335440"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10221">
        <property type="entry name" value="ASKHA_NBD_Arp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000029">
        <property type="entry name" value="Actin-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000006">
        <property type="entry name" value="Actin-related protein 3 (ARP3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6228928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="47702" checksum="03AA84AC035928DE" modified="2022-01-19" version="1">MASQTPAVVIDNGTGFSKLGFAGNDSPSFVFPTAIATKGGPGGAGGSGSGRPAVANKPSFLTGGTGSGGALSSKRGTEDLDFFIGDEALAAASGPGYGIHYPVRHGQVENWDYMERFWSNSIFKYLRVEPEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVSDRSLTGTVIDSGDGVTHVIPVAEGYVIGSSIKSIPIAGRDITYFVQSLLRDRGEADSSLKTAERVKEEYCYVCPDIVKEFSRFDREPDRFLKHTVTSPNGRSITVDVGYERFLAPEIFFNPEIYSSDFLTPLPTVVDGVIQSSPIDVRRGLYKNIVLSGGSTLYKDFGRRLQRDIKHMVDARIRASEVRSGGKKSGGLEVQVVTHKRQRHGPWFGGSLLGQTPEFRSYCHTKAEYDEIGPSIVRRFNLIGGPGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYE7</accession>
    <name>A0A8H6KYE7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156738.1">
        <property type="nucleotide sequence ID" value="XM_037298103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYE7"/>
    <dbReference type="GeneID" id="59335610"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="777"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1033"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="737"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1033"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1070"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1070" mass="120734" checksum="EC99A20E04C0E79E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYN0</accession>
    <name>A0A8H6KYN0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007361</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156887.1">
        <property type="nucleotide sequence ID" value="XM_037298252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYN0"/>
    <dbReference type="GeneID" id="59335760"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="288" mass="32407" checksum="03696EA9F127D31E" modified="2022-01-19" version="1">MARLGAAFGAEAEMLLAPKPKFSMVKGFSQLIKADYRIREHTFGCLERLTEEQTTDDAQDHARFERAFCHSLGFGTAKCVTKVRALLGHSDPRIGYLEDAVANLRKNGYHSFTNAAVEALTSTGHLGNPNLPHEYWRSDSLINVVNLSHREYRTMPETFGPVHFATSWLGSVPMHLMIEQSDYQSCENFAFRILEVSPSRATREGHWTEAELELKSFTRSLRALQWDAIDQDVVISMRNLAAVFEARGCADDVKQLELKITSVVEGISGHSYSANPLDLEWLHSLTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYR7</accession>
    <name>A0A8H6KYR7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">26S proteasome regulatory subunit RPN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_007392</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the proteasome subunit S2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156918.1">
        <property type="nucleotide sequence ID" value="XM_037298283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYR7"/>
    <dbReference type="GeneID" id="59335791"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000026">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016643">
        <property type="entry name" value="26S_Psome_Rpn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041433">
        <property type="entry name" value="RPN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040892">
        <property type="entry name" value="RPN1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18051">
        <property type="entry name" value="RPN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17781">
        <property type="entry name" value="RPN1_RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015965">
        <property type="entry name" value="26S_Psome_Rpn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPN1 N-terminal" evidence="7">
        <location>
            <begin position="67"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit RPN1 C-terminal" evidence="8">
        <location>
            <begin position="852"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00057191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR015965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6229276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="908" mass="101224" checksum="0AACC65574405CC2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZ13</accession>
    <name>A0A8H6KZ13_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157054.1">
        <property type="nucleotide sequence ID" value="XM_037298419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZ13"/>
    <dbReference type="GeneID" id="59335927"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="364" mass="40500" checksum="9ED8F17EA0FDE73E" modified="2022-01-19" version="1">MRYFDDGDLTDGLQLTPKHFPVEHVEGDYHVVWTYCKGKRRTLDWSSQIDLSNWELKFQKLPAEEGGVGRHFNVSLNFGSLELEYIMDYQDKIVNRLDLATNKAPGEGSVENEAMITFSEVRDESGYPYLRFSFSTNEHNPDDDMVNIEFVAKRTDETGVHQLGLTKNEKLRLGYAVWDDGTFEPENSDEEVVGQSADSLNKQLQRHIEASVKIHAKLVTMRNNVDKQVANATNGMQQALNQAWRSASHPDTNLQPSFDPSSAGMSPPPARRKRGEVATAEALEIPPLSQRRKKGSGPERTPISMEDDELPNGTARPAGYKRKRGEKAPGKNKGGRPPKRKSDINTVAQYLVDIAGAGSQGSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZE8</accession>
    <name>A0A8H6KZE8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase PFA4</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein S-acyltransferase</fullName>
            <shortName evidence="11">PAT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Protein fatty acyltransferase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PFA4</name>
        <name evidence="15" type="ORF">HO133_004212</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mediates the reversible addition of palmitate to target proteins, thereby regulating their membrane association and biological function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11 12">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11 12">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DHHC palmitoyltransferase family. PFA4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZE8"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03199">
        <property type="entry name" value="DHHC_PAT_PFA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033682">
        <property type="entry name" value="PFA4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11 12">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11 12">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11 12">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="14">
        <location>
            <begin position="85"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="361"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="387"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="active site" description="S-palmitoyl cysteine intermediate" evidence="11">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6229875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="433" mass="49557" checksum="F855505C257B3792" modified="2022-01-19" version="1">MSRSALGSLALPGVFALISYLAYGSQILFRHIEPHALEQKQAFIFNALVACIWITYARACFTNPGWVPPAWNSNHSASEQSPPSKKTLRWCRKCEALKPPRAHHCKICQRCIPKMDHHCPWTINCVSHRTFPHFLRFLCYATASMVYLEYFIYLRVAVVWTGRNLPSYLGPTPTQLVFLFIFLIVNSLTLFALAILLARNIWCLGGNVTTIEGWEIERHQTLVRRARVLGGYLDGPDGMRVKIRKQEFPYDIGILQNMRQGMGGTPLLWLWPFTATPSIESGLDFETNGFEDPSMSWPPPDPDRMPRLSNRPPYEQPFVYDEALSTTQNNVDAFRRRQEEDLKRYDGGATTAIRRRPFHERYRNGGHDEASFASQPVGSDDLAGGEEVWRDSEGDRLDDFGVDEDAEFYDEDDIPLAELLRRRHEPAHYSDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZZ9</accession>
    <name>A0A8H6KZZ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="4">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_004834</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157747.1">
        <property type="nucleotide sequence ID" value="XM_037295746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZZ9"/>
    <dbReference type="GeneID" id="59333240"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000005">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000014">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="14">
        <location>
            <begin position="56"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6230490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="331" mass="35996" checksum="DFDFE9CF265009B8" modified="2022-01-19" version="1">MASPNITRSREGSDSSFDSDSELFKHEIIKHDPAGLGTSKTPWQQTLAANRSTAPGDINDGLMELLIMINACKTASARRITAVIPNFPYARQDKKDKSRAPITAKLMANMLQTAGCSHVITMDLHASQIQGLSMPQTLHSSKLLIIAGFFNVPVDNLYAEPSYLRWIRDNVDVRKAVIVSPDAGGAKRATSLADRLDLNFALIHKERSRPNEVSKMTLVGNVKDKCAILVDDMADTCGTLAKAANTLREHGASSIVALVTHGIFSGNALKTLNDCDALSRVVATNTVPFSEKRKQCPKLEEIDVSPTLAEACRRTHNGESVSFLFSHAPME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L035</accession>
    <name>A0A8H6L035_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004892</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157805.1">
        <property type="nucleotide sequence ID" value="XM_037295804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L035"/>
    <dbReference type="GeneID" id="59333298"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="351" mass="39517" checksum="77161CEBEC6D943F" modified="2022-01-19" version="1">MADRDPVLEEVSNAADFLVYPLSDQAVPQAPYDPEEGIVTCVVHAINVCRGMLAHEHVRSAFIELVRQYCQTTERVWFLQGPMTEEKLEEMTDTFLKKVLDKFPLIFLDHSLYNPDSLSTHCRHAWDGEFKTSDQAIVLNGQNLAQMFTLRVIADPSDPNDFASRDYRNYLFITATSIFHEIGHVFFTYLLKGVEGTDTPPQTKAYVVGHEKKTEGESGSSLEQMVFGGVVYLYRDRDRQTPVDAVRADIVVLVQGQSFGTLADCGTPFLLTETGWYQISQDTIENTIKCSRLSLAPSSNNAFLSDFTFPFGHSDIAEDHTTLKSKGHDMPDYPGLDPQAHSEWPNVKVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L050</accession>
    <name>A0A8H6L050_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157794.1">
        <property type="nucleotide sequence ID" value="XM_037295793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L050"/>
    <dbReference type="GeneID" id="59333287"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="94" mass="10758" checksum="D8057E5572C03F31" modified="2022-01-19" version="1">MSDNDQAGESSNAAIPPHNDGPNERSKYEKNWKLDTKHVELYKEKDKVYVRGARGVDPKPYKIYKVLGEAQYKLSRDGTCDGKVYRQQDIQTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5Y7</accession>
    <name>A0A8H6C5Y7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147072.1">
        <property type="nucleotide sequence ID" value="XM_037297636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5Y7"/>
    <dbReference type="GeneID" id="59335140"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051966">
        <property type="entry name" value="RPAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46423">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46423:SF1">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="412" mass="46332" checksum="2270B1AE0E8A2D44" modified="2022-01-19" version="1">MDLQTLSSALPPPRPSLLGIPLELRNEIYRNLLSTGRNRIDLGLGRARYSLQTAVLATNRQIHDEATAVLRENRFINIITSWTSFKQDILVQGKFPIIAEGDDRSPSPESHMLVLLDFMGDVDTSRLYAYVTCLADLPHLCRLLFYASSLSPNFSSLLHVTLAIRDPQGRAEKTVVPKALQEALMMPFAVLKGLHGLTIKGARNKAVEKELRKAMGVPNPTAAEYLEAAAGLKDAGNAAFKVGDYALSIRIYIQAYEAMHFIVEGKRFAIMLDGYFASNPLTGGRFEGQRGDLVRHHLGSQLSWNILAAYLKMEDWEQAYFWGERAISDIEYADVQQSILDGTPNLVTGAEKAKVYWRMAVASQALDRKQVWARSLMKAYMFAPQDKTIMREMEALEKRLQRHELDLEDFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C690</accession>
    <name>A0A8H6C690_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="6">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_006655</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146988.1">
        <property type="nucleotide sequence ID" value="XM_037297552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C690"/>
    <dbReference type="GeneID" id="59335056"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF25">
        <property type="entry name" value="MAP KINASE KINASE PBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="285"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="200"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6217553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="608" mass="64070" checksum="27EA276440ABD381" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6E4</accession>
    <name>A0A8H6C6E4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006609</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147217.1">
        <property type="nucleotide sequence ID" value="XM_037297507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6E4"/>
    <dbReference type="GeneID" id="59335010"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="306" mass="32092" checksum="C560E8E3F4B3F8E1" modified="2022-01-19" version="1">MNPFFVAVKQALISTGRDPSPNGCPEEAKLAKSDQSRFRKVKTSRNNVSRYESLDTPNTPNTSGSDPRAFSELHPELKPRQAEWQADTPTADVSTCGYLNGNASLPRTANPGFDCRVDNKNAIWGFCSTAILAVSDCGLVGGCVDAHACDGGCGISGDPNATTVGWFVNLLVSALAILFPSLTPGTATSSSPIPTTSTITSAPASSSTHSKATSSPSNNTGAIVGGVLGGLALLAFIVVAMLFHRRLKTSPTTGGQSYDPVWRGIGDQRGAPKRVPRQTLLPAELSTGRRDETTELPNDMGGTRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C790</accession>
    <name>A0A8H6C790_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147687.1">
        <property type="nucleotide sequence ID" value="XM_037297113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C790"/>
    <dbReference type="GeneID" id="59334615"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="165" mass="18367" checksum="5788D9E04BAA48BA" modified="2022-01-19" version="1">MNLRTLDGPRNEKPSVLSKENILLIKHSSITTQTSCSDPASLGFQASTYNYNKIFRPQAPDEDLLLPDFMRNDRSTWSGSVQSMDYYSPYQATPLATNLGATHQVPRSAKDTISGYNTVEGAESHPKRGSRKLRDAGTNTEGINRGDPQSQPRMKNQKPLARKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7D2</accession>
    <name>A0A8H6C7D2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147743.1">
        <property type="nucleotide sequence ID" value="XM_037296562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7D2"/>
    <dbReference type="GeneID" id="59334059"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037652">
        <property type="entry name" value="Mim2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28230">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28230:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19117">
        <property type="entry name" value="Mim2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="120" mass="13159" checksum="473CC047BA1BBD6A" modified="2022-01-19" version="1">MSSTSSSSQYIYPPSPFPPPSPSPSSDLDSLPDSAPSDDTTSTSDDEGESDAEKEWKESLQQLELILTMVLVPYAGKYFGRKCAYWGWAKFMQWKYPVEVVFTSKKTFKAAGAVEAAASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7F3</accession>
    <name>A0A8H6C7F3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TIM-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147712.1">
        <property type="nucleotide sequence ID" value="XM_037296531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7F3"/>
    <dbReference type="GeneID" id="59334028"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009215">
        <property type="entry name" value="TIM-br_IGPS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051353">
        <property type="entry name" value="Tobamovirus_resist_UPF0261"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31862">
        <property type="entry name" value="UPF0261 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G10120)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31862:SF1">
        <property type="entry name" value="UPF0261 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G10120)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09370">
        <property type="entry name" value="PEP_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIM-barrel" evidence="1">
        <location>
            <begin position="66"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="337" mass="36312" checksum="13652E9E38D4F085" modified="2022-01-19" version="1">MERTMTSSDVETHGKPTPFITVTFGKWPTPDIPVLDTVHQESTISSYEVEIILLANMPRPTNRIEVLGRLRETIAAGKAILGAGAGIGLSAKFVEKGGADLIIIYNSGRFRMAGRGSLAGMMPFGDANAIVLDMATEILTVIESTAVMAGVCATDPYRSMPQFLQQLKDLGFCGVQNFPTVGLIDGTFRKNLEATGMSYQKEVEMVRIARRLDLLTAPYVFNVDEAERMTKAGADVIVVHLGLTTGGSIGAGEGEITLDDCVSIVQTIRDACVKIEPDIIVLCHGGPVSGVEDTRYVIDRVDGIHGFFGASSMERLPAKTAIEQNARKFKNIEIGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7M3</accession>
    <name>A0A8H6C7M3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147708.1">
        <property type="nucleotide sequence ID" value="XM_037297134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7M3"/>
    <dbReference type="GeneID" id="59334636"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="330" mass="37910" checksum="3CF3B47B17E11BFC" modified="2022-01-19" version="1">MKQEPVWKVGPVMESIYSTLQQILRLVEKSPKIYLDDAFLSEISVRKHEWDLADTDDFFACMTEEELAHLRPSDPIPDEAIPYEDVEYPESAQPQNVFDLESSGIAIELWFHELQERKVSIDGCGCIWPEGSSSWAPRELFKYEDLCHGRRNPPHVPWTLSGAYYNDDQPHQIYHAWHGDEGREGIILRSELQILIRCMKGRMADPALCMHNVPVLLLSIHGTKARIMIAHFNMKRKTLDIKCSGFEQFDGFEAKENTGTTDGCARPHDFEAKRDLFFRWLNPICQGDTYLKDSEDAPEMVLRGPKGRKRLVPRHLPPPSSSSESTEILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7Y0</accession>
    <name>A0A8H6C7Y0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147939.1">
        <property type="nucleotide sequence ID" value="XM_037296758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7Y0"/>
    <dbReference type="GeneID" id="59334258"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="158" mass="17682" checksum="0ECA85964FEA1F28" modified="2022-01-19" version="1">MSPVLDTLSTILSDLDLVSSSGRYDAATGFKKTQAGFYDCFDKLMAASKSLIAAYHSMLEMHKDCARMINQLLALGQPNSVVVQHIRARKAQIEQDMLEMESIQCETMRSIQLLHDGNQLVIRILRRLKQPEEENQKGCKRKADEMDGESDSGSSEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C847</accession>
    <name>A0A8H6C847_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U3 small nucleolar RNA-associated protein 15 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005795</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147881.1">
        <property type="nucleotide sequence ID" value="XM_037296700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C847"/>
    <dbReference type="GeneID" id="59334200"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018983">
        <property type="entry name" value="U3_snoRNA-assocProt_15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924:SF26">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924">
        <property type="entry name" value="UTP15 U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09384">
        <property type="entry name" value="UTP15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="128"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="171"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="256"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 15 C-terminal" evidence="8">
        <location>
            <begin position="382"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="557" mass="61444" checksum="77EF5233BF20AC2F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C893</accession>
    <name>A0A8H6C893_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal RNA methyltransferase FtsJ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148225.1">
        <property type="nucleotide sequence ID" value="XM_037296243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C893"/>
    <dbReference type="GeneID" id="59333739"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="2">
        <location>
            <begin position="80"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="393" mass="44100" checksum="ECDCD806442D0595" modified="2022-01-19" version="1">MDEISGEPERSPEDQIIASFLKERSPTYRSLREISTIVWSDSRATAYFARERGSADNPGDEGRRKHYNLMRGIGDEMQRETGVLELQRPRPEVLDLCMAPGGYTASIRKYSRQANVSAITLPEDQGGLKILVPHGNRTQVEVLEADIIMFAAEFGVVEIPDNHPEASKFSNEKPFQKKLFDLVFCDGHVTRTRNRPSNCPPTELSRLECSQLVLAMQRIREGGTLIILLHGADVYHSVKLLEIFGHFSHIQVFKPVRGHTRKSSFYLVAKEMQPGRLAARAAVTQWKQNWKDATFPAPAEDEIDAHNETEALLKQVERKEDSSKLVENFGARLIQLGEPIWEIQEKALKALMERLEQGSAKPGMEKSAETSEECTTADPAVLTGLARDTDDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9A1</accession>
    <name>A0A8H6C9A1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005092</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148702.1">
        <property type="nucleotide sequence ID" value="XM_037296004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9A1"/>
    <dbReference type="GeneID" id="59333498"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17781">
        <property type="entry name" value="CBS_pair_MUG70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17782">
        <property type="entry name" value="CBS_pair_MUG70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06409">
        <property type="entry name" value="PB1_MUG70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051462">
        <property type="entry name" value="CBS_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108:SF26">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN DDB_G0289609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="648"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="100"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="167"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="270"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="336"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="6">
        <location>
            <begin position="479"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="450"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="579"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="456"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="673" mass="72875" checksum="FDAEADD19E0B45E7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9V8</accession>
    <name>A0A8H6C9V8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heme-dependent catalase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149052.1">
        <property type="nucleotide sequence ID" value="XM_037295005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9V8"/>
    <dbReference type="GeneID" id="59332495"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36195:SF4">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G01990)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36195">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G01990)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="42481" checksum="E116AD22F7C23046" modified="2022-01-19" version="1">MDAVKDSLTQGYNTAKRALSTAPPNDAYLHWDAPGVEEIKPDEEAKAQQIADTMNKMQKHNFDKHRHAFRATHVKTQGLVKGTLTVPSGLPPHLSQGLFAHPGTYPIASRYANEPVFLQSDTEPGPRGMAMKIFNIHGERLETPGNKSLSTQDFFFNNAPMIELTDIDTCLEIMQLRERYFDNPNGLAMALKLRTDIIKQHAPGMLPNTNMISHSMYTQSAFRFGEYYGHMAMFPVLPEMKEKGGAAVLSSGSYTQISDWLFDYFRGAPAKYEVKIQLGTSPAHHPTEDASIVWDEVTAPYQTIGTVEFPVQDSFSQARRVFWEDHMVLDPWRALAAHRPLGSINRLRKVVYARSQKKRDELNAKKSSNIQSIDEMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Y1</accession>
    <name>A0A8H6C9Y1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SPIN90/Ldb17 leucine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148804.1">
        <property type="nucleotide sequence ID" value="XM_037296106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Y1"/>
    <dbReference type="GeneID" id="59333601"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030125">
        <property type="entry name" value="SPIN90/Ldb17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018556">
        <property type="entry name" value="SPIN90/Ldb17_LRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357:SF1">
        <property type="entry name" value="NCK-INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357">
        <property type="entry name" value="SH3 ADAPTER PROTEIN SPIN90 NCK INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09431">
        <property type="entry name" value="SPIN90_LRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPIN90/Ldb17 leucine-rich" evidence="2">
        <location>
            <begin position="185"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="463" mass="53025" checksum="A15D97F74A2204F2" modified="2022-01-19" version="1">MEFEVSYTLDNEQQFWDELEEIVSAQCQSHELIDNALRSYLTFVTNYKGEYVQTEYDIARCSYRLLESSLFIENKDYVRRQIVYSLLQEDSPDTLHLIIAFLLFDGQQNDITFEMMKEEGGFPRLLELIQEGRDDDAGLHRMLLEVLYEMSRIQRLRIEDLILIEDEFVAYMFQIIEGLSDDVNDPYHYPVIRVVLILNEQYMCSAHDPSPNQTTSPSTPLTNKVMKILSAQGSAYKTFGENIILLLNRESETSLQLLILKLLYLLFTTPPTYEYFYTNDLHVLLDIILRNLLDLPPSSTALRHTYLRVLYPLLAHTQLKHPPHYKRNELVRLLAMMAEDDNQHFGAVDETTKRLVERCGRVGWLKDEEASGSIPTWMKQRLLSVDLPSAMKSSLSVVEVTTQREKPGVKTPSRTDDDRANENTGNIVDSSGTINNGAVTAGNDGIGRAMGVEKSPFEVEGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Z4</accession>
    <name>A0A8H6C9Z4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005217</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148826.1">
        <property type="nucleotide sequence ID" value="XM_037296128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Z4"/>
    <dbReference type="GeneID" id="59333623"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14019">
        <property type="entry name" value="STKc_Cdc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF23">
        <property type="entry name" value="CDC7 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="90"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="27"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="475" mass="54139" checksum="373BCE6C727A7FC5" modified="2022-01-19" version="1">MALARPAVPIAEKNPFITSKHLTPANKLSHKLASSPGSQYDTDEYEEGEDEQEGSECSEESAESEYAVDETVREDMASLEDTFREIGMRFRMIARIGEGTFSTVYKAEDLHYEYYQNDWDVEQRDASKWTSPPLKRRKNASTLETSHDQCRQPKRRFVAVKKIYVTSSPMRIQNELELLHDLRGCKAVCPLITACRHQDQVVAVLPHFRHQDFRAFYHSMGHNEIKVYFRSLFEALAAVHKHGIIHRDIKPTNFLFDVERQRGVLVDFGLAEREGTECSHCLCQEQVNIRKAKIQSSRAYSMPTPTGYPKDDQRPSRRANRAGTRGFRAPEVLFKCTAQTTKIDIWSAGVILLTILAQRFPFFHSGDDVEAMIEIATIFGKQRMRGCAYLHGAVWDCNIPTIGEKGFPLSQIVQWSTSEKVGEHMDQETKETVKFLEQCLQLDPSKRMSARAALASDFLAEETYPDTEVEEVGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA36</accession>
    <name>A0A8H6CA36_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148821.1">
        <property type="nucleotide sequence ID" value="XM_037296123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA36"/>
    <dbReference type="GeneID" id="59333618"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="561" mass="60062" checksum="072304B35E755D1B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAG8</accession>
    <name>A0A8H6CAG8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149440.1">
        <property type="nucleotide sequence ID" value="XM_037294752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAG8"/>
    <dbReference type="GeneID" id="59332241"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="223" mass="25539" checksum="1465B963655E3D70" modified="2022-01-19" version="1">MTECLMECVMKKQCRLACFGALSKVETFSHGYEKTTVVCFSITHVNWSRQGASAQKRRGGLSALSVRRLEPRWHMERGRTPRLGLDLSQFYAKGDREPSLFEVPRPTMATTGRTPDHNPLKRLKIEDEKDLIKNLSIAQAFSTEYSNDDLSKAIAFRYPDKCSTIRGSDVQSIFSYLEANPHCSRDYARFKSMAARNKYGNGIREVQDALREWDVDIDMAKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAJ5</accession>
    <name>A0A8H6CAJ5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149208.1">
        <property type="nucleotide sequence ID" value="XM_037294520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAJ5"/>
    <dbReference type="GeneID" id="59332009"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="87" mass="9877" checksum="C35B4CCC1A35FA88" modified="2022-01-19" version="1">MATGTAPKRLRFDLFTEHVDNTHSEPATKWHSTHSILITYLFITPTNRQVHEFTEVVYAYGVPAVEAVGLQGGWMQWIALRSKMAMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAW8</accession>
    <name>A0A8H6CAW8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1,4-alpha-glucan-branching enzyme</fullName>
            <ecNumber evidence="4">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glycogen-branching enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_003734</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Glycogen-branching enzyme participates in the glycogen biosynthetic process along with glycogenin and glycogen synthase. Generates alpha-1,6-glucosidic branches from alpha-1,4-linked glucose chains, to increase solubility of the glycogen polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149344.1">
        <property type="nucleotide sequence ID" value="XM_037294656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAW8"/>
    <dbReference type="GeneID" id="59332145"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02854">
        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000001">
        <property type="entry name" value="1,4-alpha-glucan branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000250">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000003">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="13">
        <location>
            <begin position="226"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6219909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="713" mass="81871" checksum="465C2BA10190C9CC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAW9</accession>
    <name>A0A8H6CAW9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149514.1">
        <property type="nucleotide sequence ID" value="XM_037294133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAW9"/>
    <dbReference type="GeneID" id="59331621"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="558" mass="63350" checksum="6ED538B46523D442" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAZ3</accession>
    <name>A0A8H6CAZ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin-related protein 2/3 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003779</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149389.1">
        <property type="nucleotide sequence ID" value="XM_037294701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAZ3"/>
    <dbReference type="GeneID" id="59332190"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1760.10:FF:000002">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.10">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007204">
        <property type="entry name" value="ARPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036753">
        <property type="entry name" value="ARPC3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12391">
        <property type="entry name" value="ARP2/3 COMPLEX 21 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04062">
        <property type="entry name" value="P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016315">
        <property type="entry name" value="ARP2/3_P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69060">
        <property type="entry name" value="Arp2/3 complex 21 kDa subunit ARPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="188" mass="21183" checksum="EA8359DCD2BBE176" modified="2022-01-19" version="1">MPAYHSIFLQDQNVRLIGNFALLPLRTRTRGPAYSLPALAPGTSELEIDPDSESYDSLDEVLSLFRANTFFRNFEIKGPADRLLIYGILFLGEALGKIKPTLGKKEAEKALLNAALDQFAIPGDASFPLNQAFEAPRDRQDAETLRQYLSQVRQELAMRLLARVYADGNTPSKWWLSFTKRKFMGKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCK3</accession>
    <name>A0A8H6CCK3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150171.1">
        <property type="nucleotide sequence ID" value="XM_037294093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCK3"/>
    <dbReference type="GeneID" id="59331581"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034572290" evidence="1">
        <location>
            <begin position="21"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="317" mass="32777" checksum="2BF8A237D8C7908A" modified="2022-01-19" version="1">MRPTTLLPTALLLLIHSASAAFSLNDTGPYWSYTARQLSPTTDRKCLTAYAAPIACTETLLGLVTSLRPDFQPTRDDLSSMCTPGCKSALEDYVQNVGSACGAKGDLATQRAVKSGKNNTYTGPPPPSFLAPQILNLENKKKKKKKKKQKKKLTAPRPTAFCPLTSTTHPSAAANCSSPCSVAFYTNAHDFAPASRKQFQDSYLTTQTAYWIERFNLGYRALVACGEAVGNAERTGSAGAGFAPRSTGDVTGDVTGGGVAAVATTTTMGSPAGTSAGSAAAVTVAESGRSGGSVLEVDVKTVIALGVLGLVAAAVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCP0</accession>
    <name>A0A8H6CCP0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycerol-3-phosphate phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150444.1">
        <property type="nucleotide sequence ID" value="XM_037293615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCP0"/>
    <dbReference type="GeneID" id="59331102"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050308">
        <property type="term" value="F:sugar-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07527">
        <property type="entry name" value="HAD_ScGPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000013">
        <property type="entry name" value="Glycerol-3-phosphate phosphatase (GppA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051806">
        <property type="entry name" value="HAD-like_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481">
        <property type="entry name" value="FRUCTOSE-1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481:SF4">
        <property type="entry name" value="GLYCEROL-1-PHOSPHATE PHOSPHOHYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="26440" checksum="0D3382EE00B70CBF" modified="2022-01-19" version="1">MPLLEENPAFTGRPETHKFAGILFDMDGTIVDSTDAIVKHWHKIGKELGVDPAMILQSSHGRRSIDTFQLYDPSKANWEYVRHVEGLIPRQFGLDAVEVPGARNLLASLEAAKAPWAIVTSGTRPLVTGWLDVMKLAHPRNLVVAEDVKEGKPDPECYRLGREKLGLSADTRVLVVEDSPAGIRAGKAASCNVVGLATTHDIAQLRENGPDWIVKDLQSVRFVNRNADTGEVAIEILHSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD12</accession>
    <name>A0A8H6CD12_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_002210</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150790.1">
        <property type="nucleotide sequence ID" value="XM_037293137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD12"/>
    <dbReference type="GeneID" id="59330624"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053045">
        <property type="entry name" value="Zinc_cluster_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986:SF7">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="495" mass="55943" checksum="D4C7B1B187749071" modified="2022-01-19" version="1">MASKDESSKTQRTISPQADHTSPKKRRKVNHACIYCRRSHMTCDLDRPCARCVKRSIGHLCHDEQRDSGKGMKNDQANTTSNSGAAVKQENSLPYMLSPSVDQQQLGHQALHDSGANVITRTAAPDSQADNPQFLPQSSDPISQGQGQVFGDKNQQFPGYGDWSLGNQNQFSDMHSFHPSYMFNAPDITNEYNLLNEFLSSSLLDDPTVFPNDEIQALSSDTSLSNTLAASLNGGSGQNLLNSQQTQASGLIQGPLGLDISIARPKSVKPTDKEEEKYYMTVADPSGNDTPEERMNKVFMAKYNAGMLRPFNYIQGYARLHRYMDQNMQPASRQRILRQLDKFRPKFRERMQSLTDMELTFVEMWFERSLMEYDRVFASMAIPACCWRRTGEIFRGNKEMAELIHVPIEKLRDGKLALHEIIVEKDLVSYWDKFLAIAFDNTQKAMLTTCSLKNPDLKASDPEIKCCFSFTIRRDNHNIPSLIVGNFLPQGPRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDA0</accession>
    <name>A0A8H6CDA0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fucose-specific lectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150822.1">
        <property type="nucleotide sequence ID" value="XM_037293169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDA0"/>
    <dbReference type="GeneID" id="59330656"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.70">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89372">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="51006" checksum="AB2D53E9472C7391" modified="2022-01-19" version="1">MQMSDRDFYRAPEVGQVHSTLEVGHQPDPPQIYSTLESRQPDFSLFDTYKQSTTVLPQPEISDASLDTPSNIESDNHTTLVREGRGPRKRAIWILVALGIVIISAAAAIGGGIGSTRTSHRSVSSGSLSDQAPSSTMTKMSSSAKSSSTIISSVSIPTMSTASSFPTPAAKLNTTLLNTTKLASVVWNINGVTEYRMWYQTEDNMIREVGKNDTGNQWYTSGQSHGPAKRGSPIAASYTGPPDWPIEISLFYLSEDGSIIFTKFEEKDQSWSPSSLSEIPADGSNLATLWFRHDECNDCSNDLLLVCQNSDDKLALFNSTANAGPQWITLDAYPMPGTGLAFNLVHGTYDTGGLFVVYQTANQALCSASFNKSQGWTVNEGNPISELSTQAPLAGFTWNVSGAEYLDIVSTGPSGVTVNYFNFTNGDWTSVPSSGVLAQVQNYSSIAANAASHIYAFQDGSVKEYQLAPAGSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDM4</accession>
    <name>A0A8H6CDM4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002388</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150967.1">
        <property type="nucleotide sequence ID" value="XM_037293314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDM4"/>
    <dbReference type="GeneID" id="59330801"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00048">
        <property type="entry name" value="DSRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DRBM" evidence="2">
        <location>
            <begin position="7"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="168" mass="18401" checksum="5E25B8739D289BED" modified="2022-01-19" version="1">MYYQEYLSNLCQRRQWPEPIYRLFRAEPGFVCVARVNNREYQSDTIHENERLAQENAAMRAYLICQNFSKSDGMYPSGHKGRRGGDVVQGLPVAIGTERMSVYTDDSRSSGSRSGGSSPTTAESGHSYGSSYGSQSYGGCRRLSAGSGVGSGGGGYASRTSRHRSGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDS3</accession>
    <name>A0A8H6CDS3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002234</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat MET30/SCONB/SCON-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150814.1">
        <property type="nucleotide sequence ID" value="XM_037293161.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330648"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22147">
        <property type="entry name" value="F-box_SpPof1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="YTH" evidence="7">
        <location>
            <begin position="371"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="1029"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1292"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1360"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1407"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1447"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1489"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1529"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="668"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="794"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1108"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="675"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="740"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="821"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="831"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1114"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1136"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1153"/>
            <end position="1175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1643" mass="181589" checksum="7F530CA36D414155" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDW7</accession>
    <name>A0A8H6CDW7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Got1-like family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001711</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GOT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151178.1">
        <property type="nucleotide sequence ID" value="XM_037292639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDW7"/>
    <dbReference type="GeneID" id="59330125"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000137">
        <property type="term" value="C:Golgi cis cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF9">
        <property type="entry name" value="GOLGI TRANSPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="14922" checksum="CA7CA654A77ABE40" modified="2022-01-19" version="1">MPTMWLSDSQKVGVAFCSGGGFFLVGGVIMFFDRAMLAMGNILFLLGLTLIIGLQKTAVFFARRQKLKGTAAFAAGIILIVLRWPLVGFLIELYGIFVLFGDFFSTIGGFAGNIPVVGPYIQQALVRAGGGRRNAELPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDZ7</accession>
    <name>A0A8H6CDZ7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002297</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150876.1">
        <property type="nucleotide sequence ID" value="XM_037293223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDZ7"/>
    <dbReference type="GeneID" id="59330710"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034914287" evidence="3">
        <location>
            <begin position="18"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="264"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="530" mass="58335" checksum="0AE8EEE87EF00D2B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE26</accession>
    <name>A0A8H6CE26_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151145.1">
        <property type="nucleotide sequence ID" value="XM_037292606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE26"/>
    <dbReference type="GeneID" id="59330092"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="448" mass="50274" checksum="272A9A6EFBBC9EC4" modified="2022-01-19" version="1">MKARATAQPSPRFLTCALELRENIYAHILRNHSSSLVDLLVVNRQLCREVKPFLYKLPLTFDGQSQLFDWLSKVDHGYLRHVEEIGFKLHDINPDEIVGALGKKLQNANITRASGSRGPDTPDNPYFEACRADLRRIGAAFRLLPNLRYLTILSASDRDSRPHHRTQDAFSKLVGQCFPKLKSLISEEDQFPIDFVSGKPELRRLRFPATTSSDDEETEGLFSALSLSELEICRLPHHTASGAEEWDCVTPILEGSPSLQSLTLFERHGAETPNLAAEVFVGSQAALRRHRSSLRTLKLLAKPGPEYSADEDSDPVHPEACCAAFLAQSSLTHIEFTDVWMSVYEQLPRTIQTVTWRLDESPRPPNVATSVARIKKMVTHAVRARGNPRDPTLPHLSRISIHLDGFFPAADIRHPDHPSREILMMARAKLGDLGVRLDWKGWEGVPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE27</accession>
    <name>A0A8H6CE27_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ADP-ribosylation factor-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001725</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151192.1">
        <property type="nucleotide sequence ID" value="XM_037292653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE27"/>
    <dbReference type="GeneID" id="59330139"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04151">
        <property type="entry name" value="Arl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000624">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="19861" checksum="9B9C525F4707BF77" modified="2022-01-19" version="1">MGGSVSRILSLLWSKKEIRILILGLDNAGKTTLLYRLKIGEVVTTIPTIGFNVESVVYKNLNFNVWDLGGQTSIRPYWRCYYANTAAVVFVIDSTDIERLNTAAEELAAMLNEDELRDAGLLVFANKQDQPGAKGAGEISEALRLGELRDRNWSIVACSAIDGSGIAEGMDWMVQTVNSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEP1</accession>
    <name>A0A8H6CEP1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001963</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151430.1">
        <property type="nucleotide sequence ID" value="XM_037292891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEP1"/>
    <dbReference type="GeneID" id="59330377"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11063">
        <property type="entry name" value="CYP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF17">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="514" mass="58840" checksum="53A91C1C4BCFA33A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF41</accession>
    <name>A0A8H6CF41_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151489.1">
        <property type="nucleotide sequence ID" value="XM_037292070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF41"/>
    <dbReference type="GeneID" id="59329556"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="170" mass="18283" checksum="47D8856220208AEC" modified="2022-01-19" version="1">MAASTTTTTPALFRFSVHNTTDTTHISHTHPVPLFLCQPAESQQTSAAFKDAFIDVIALITAQHHTECMAALTSPCLACASPTTDCLKSPSSFLNLAEPVVVIAVYPVCGSKTCEYQVRGQLLRRQMRGIEAAKEDERKLYGKMGREALCWVWLGGVLRQGVSGGGVEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFS5</accession>
    <name>A0A8H6CFS5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001485</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151834.1">
        <property type="nucleotide sequence ID" value="XM_037292415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFS5"/>
    <dbReference type="GeneID" id="59329901"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46206">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="195" mass="21405" checksum="A85B1D9E3D7D88AD" modified="2022-01-19" version="1">MASSLHGGRRQRIHDSSVSSRASAIPASSAPSHPPIPTEYSGDSHDEKSNGNDTASPLLTWTYEDAFNDLERDPVNLAHRMIFLGLGSIHTTGSQTAHAIHDLAARPELVKSLNEEIEKVRTAEKGWEKAALVKMWRLDSFMSESKRMNPPALTVFHRLAMELLVLFDGTRILKGTFIAVAAASILLDPDVVRDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFX8</accession>
    <name>A0A8H6CFX8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kri1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KRI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152186.1">
        <property type="nucleotide sequence ID" value="XM_037291824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFX8"/>
    <dbReference type="GeneID" id="59329309"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="3">
        <location>
            <begin position="535"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="229"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="339"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="433"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="598"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="507"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="606"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="663"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="677" mass="77783" checksum="CF432FA6283ABD73" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH15</accession>
    <name>A0A8H6CH15_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000174</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152549.1">
        <property type="nucleotide sequence ID" value="XM_037291114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH15"/>
    <dbReference type="GeneID" id="59328593"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14858">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="entry name" value="tRNA modification GTPase MnmE domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714:SF2">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="GTP-binding protein TrmE N-terminal" evidence="6">
        <location>
            <begin position="14"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="MnmE helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="G" evidence="5">
        <location>
            <begin position="210"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="515" mass="56233" checksum="5B76E865DABAF651" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIE7</accession>
    <name>A0A8H6CIE7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-alkyl-2-acetylglycerophosphocholine esterase</fullName>
            <ecNumber evidence="1">3.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010749</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.47" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153235.1">
        <property type="nucleotide sequence ID" value="XM_037301601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIE7"/>
    <dbReference type="GeneID" id="59339139"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003847">
        <property type="term" value="F:1-alkyl-2-acetylglycerophosphocholine esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272:SF0">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="323" mass="34373" checksum="C6F24DD5EBCC8360" modified="2022-01-19" version="1">MHTGYHALIGAAADIPTNPPKPIISFSPVVLPAPGRPVDLQLRVTVPSTGDALPIILLSHGQGRSNSLSSLEGYAPLYEFWAAHGFAVLQPTHLSSAFLGLEAPEGQELFWQERAADMVRILDHLNSVEAAVPGLRGRLDKRRVAVAGHSAGGWTAAMLLGASNTDPRDGSTWYEPETRIKAGVVLAGIGNGGADISDFGKKLVPFYGANFSNMATPALVVYGDEDVSPHLTIRGADWHADPYALAPGPKDLLTLKGAKHILGGISGWDASETLDQSPERLAVVQRMTWAYLRSQLYEGDGAWAEACKALEGLEGQGKVESKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIL5</accession>
    <name>A0A8H6CIL5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153325.1">
        <property type="nucleotide sequence ID" value="XM_037301691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIL5"/>
    <dbReference type="GeneID" id="59339230"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="222" mass="24737" checksum="33FFC34756E29C04" modified="2022-01-19" version="1">MEHPNAGKKPGYQSRTVPQYRTSPSYAGPNSYSRTTSQYYTVPCQPMMADLPVRENPRTTKVPAAKIPNAEATLKVLQIPCDGSHMKLISVPLLVISPEIVESNHSDGLESHLRHVPKLCNKGAMSFEWHSRSLDTMSAAQLGPNWKEDYFMYKCNDSKAGLKANAFFARVHGARVYGDAFVFRMEPDTYDGFTGRAEYVDMDHGFAKQTGAMELLDNLAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ48</accession>
    <name>A0A8H6CJ48_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_011080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153563.1">
        <property type="nucleotide sequence ID" value="XM_037301929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ48"/>
    <dbReference type="GeneID" id="59339470"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="132" mass="14849" checksum="0581E42795025B4F" modified="2022-01-19" version="1">MFVDIGGGMVHEGLALKTRYPNPPGRFVNQDLPQIVSDQKLHGIESMAHDFFTPQQLKDPSHSKVLINQWLVPTQRASFMTHQDPNTMATFSPMERTEEETRHLPDGAGLRVLRVWRPDDGKSECVAEAGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ67</accession>
    <name>A0A8H6CJ67_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010912</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153395.1">
        <property type="nucleotide sequence ID" value="XM_037301761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ67"/>
    <dbReference type="GeneID" id="59339302"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000096">
        <property type="entry name" value="DnaJ domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051964">
        <property type="entry name" value="Chaperone_stress_response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="9"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="162"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="197"/>
            <end position="217"/>
        </location>
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            <begin position="276"/>
            <end position="287"/>
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        <location>
            <begin position="343"/>
            <end position="354"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="501"/>
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    </feature>
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        <location>
            <begin position="517"/>
            <end position="527"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="542"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="605"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="720"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="778"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="826"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="976" mass="107289" checksum="C7B8F7425838D4F1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJM2</accession>
    <name>A0A8H6CJM2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153469.1">
        <property type="nucleotide sequence ID" value="XM_037301835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJM2"/>
    <dbReference type="GeneID" id="59339376"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="322" mass="35782" checksum="4747884A103BA794" modified="2022-01-19" version="1">MALTRSFTFPLSHTEESSRTPPFSLNHEDVGRLLSCRAFQAIRGFLTSRHDDSFCLHHQPTSKAHEEAWLLQRDITYALILPLLEIQRYATQLAISMLGDRNIFDLELVFRGEARGAFAWLQCFIAEEEEWCSAQGCPACVVSHVLEAEPTIRIIVTACRLASSLRRVSDPTPGEDPSSLPVFDFWLSSLRTALDEDPFWGPDIWKELEPRASNLENGLHQMVRQCIEFEEMQATKTVRQPELAPISASVGAAVLPAKLGTYRSDGKRMVFIGEESSWMQKIVLGCLTTLLADMANVGRTPGVAATLHATAVISPLSQPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK00</accession>
    <name>A0A8H6CK00_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRO domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010067</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153740.1">
        <property type="nucleotide sequence ID" value="XM_037300923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK00"/>
    <dbReference type="GeneID" id="59338459"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09242">
        <property type="entry name" value="BRO1_ScBro1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09237">
        <property type="entry name" value="V_ScBro1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="8">
        <location>
            <begin position="5"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="791"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="865"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="734"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="794"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="865"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="903"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="922"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="960"/>
            <end position="970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="979" mass="108984" checksum="21F101B2E803E0AC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK27</accession>
    <name>A0A8H6CK27_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diacylglycerol O-acyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_010255</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Sterol O-acyltransferase that catalyzes the formation of stery esters.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.20" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153927.1">
        <property type="nucleotide sequence ID" value="XM_037301110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK27"/>
    <dbReference type="GeneID" id="59338647"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF7">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6225060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="435" mass="48424" checksum="A73856DF0BD1E793" modified="2022-01-19" version="1">MAAIARTLSPPEIASHDATPAHANGSVLSSNSSPVNGNATGPITRDISSKYRHIAAVHSRARTSCLSRDAELTPSFLGFKNLMVIVLDILLGIVLYLLVPCHLFVAYIIELAAAHQAKGALGRAKRHSEDPPDSAQAAKSFRTTWLTIAWAHGINATLSLLVTSLTVYNLIHHPLIGTISEVHAIIVWLKICSYAFTNRDLRHALLKADSASSIPELYSVCPYPRNITLSNLSYFWWAPTLVYQPVYPRTSTIRWPFIAKRLAEATSLSIFIWLASAQYAAPLLHNSVFKIASLDLPSILERLMKLSTISLVIWLAGFFAVFQSVLNALAEIMRFGDREFYTDWWNSPSVGTYWRTWNKPIYHFMKRHIYAPLVGRGWSSNAASAWVFVFSGVLHELLVGVPTHNILGQPLAALLYFFAWQAKYGSVSKNQIYSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL77</accession>
    <name>A0A8H6CL77_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010348</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154018.1">
        <property type="nucleotide sequence ID" value="XM_037301201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL77"/>
    <dbReference type="GeneID" id="59338739"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="225" mass="25284" checksum="A324576B039E674E" modified="2022-01-19" version="1">MSTLAQPKSRTALTPPATVECQTVWEETPTRRPEAGEGDIISITDIIIIGQASLSQAPHYMAAAKVTWGRWEANSGCTYYPDPLMVIRFRYLRMSDATRNEVQGRDLDIDEERKYKWYNAEPKIVTAAADHNNWMLRIEFGGAMVTDNVSSCKQTREVKLWGKRVVGDEEVKLGRAGKDRLQIDSNVFLAELRPKESGSLCSDGVGHAAVRWLEIKTVDPLWNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLC5</accession>
    <name>A0A8H6CLC5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154356.1">
        <property type="nucleotide sequence ID" value="XM_037300508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLC5"/>
    <dbReference type="GeneID" id="59338042"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="74" mass="9002" checksum="DA58FCCF6869A3C1" modified="2022-01-19" version="1">MSILASPREEEEEDEGEEEEEEEEEEEEEEEDEEDEEEEEEEEEDEEDEEGNIYTEPDDHMDPGCWQKFVKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLR9</accession>
    <name>A0A8H6CLR9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase II subunit 1</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">ATP-dependent DNA helicase II subunit Ku70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_009594</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ku70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154303.1">
        <property type="nucleotide sequence ID" value="XM_037300455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLR9"/>
    <dbReference type="GeneID" id="59337989"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00788">
        <property type="entry name" value="KU70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01458">
        <property type="entry name" value="vWA_ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1600.10:FF:000004">
        <property type="entry name" value="ATP-dependent DNA helicase II subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000071">
        <property type="entry name" value="ATP-dependent DNA helicase II subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.970.10:FF:000003">
        <property type="entry name" value="ATP-dependent DNA helicase II subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000001">
        <property type="entry name" value="X-ray repair cross complementing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.970.10">
        <property type="entry name" value="Ku70, bridge and pillars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006165">
        <property type="entry name" value="Ku70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027388">
        <property type="entry name" value="Ku70_bridge/pillars_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047087">
        <property type="entry name" value="KU70_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005160">
        <property type="entry name" value="Ku_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
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    </dbReference>
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        <property type="entry name" value="ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF2">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ku_C"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SAP"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00513">
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        <property type="match status" value="1"/>
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    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="12" id="KW-0779">Telomere</keyword>
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            <begin position="615"/>
            <end position="649"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="562"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
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    <feature type="active site" description="Schiff-base intermediate with DNA; for 5'-deoxyribose-5-phosphate lyase activity" evidence="20">
        <location>
            <position position="26"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024890"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR003033-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6225594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="652" mass="72979" checksum="3D510D6247523219" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Pezizomycotina</taxon>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <begin position="219"/>
            <end position="243"/>
        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6225806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="512" mass="56353" checksum="1B010FA8CC5BA946" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name type="scientific">Letharia lupina</name>
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        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
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        <location>
            <begin position="17"/>
            <end position="786"/>
        </location>
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        <location>
            <begin position="130"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
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        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="786" mass="84651" checksum="78CB045523E56878" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNU4</accession>
    <name>A0A8H6CNU4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009367</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155054.1">
        <property type="nucleotide sequence ID" value="XM_037300228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNU4"/>
    <dbReference type="GeneID" id="59337762"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48187">
        <property type="entry name" value="LD21810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48187:SF2">
        <property type="entry name" value="LD21810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="179" mass="20067" checksum="839BE85A44E0214A" modified="2022-01-19" version="1">MFSTARRLVSAVTPKLVVDTDGQLLNALREGSETLQNVTDQFAPLMKRFRIYFFWEQEKTTILHSKAYEIVDESSAAPILENTERSGIAADHSHMCKFENSDAPGSRIVVAALMRYARESPALVESRWSQAKEMLRTQRSAEATELTGVGVLAERVYPTAFFATIQRVAVRHIYALGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNX0</accession>
    <name>A0A8H6CNX0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial import receptor subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_008324</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155192.1">
        <property type="nucleotide sequence ID" value="XM_037299191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNX0"/>
    <dbReference type="GeneID" id="59336721"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03078">
        <property type="entry name" value="GST_N_Metaxin1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF41">
        <property type="entry name" value="FAILED AXON CONNECTIONS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="7">
        <location>
            <begin position="21"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="8">
        <location>
            <begin position="212"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6226883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="48571" checksum="9703363A4E975724" modified="2022-01-19" version="1">MVLELHIWGPAFSLPSIDPQCLTAIAYLAQALPRDQWVLVASSDATLSPTKELPVLHDGSATIGGFENIVDYLRKRSDGDWDIDRQFSKPKDRADVTAFSSFLTFQAHPLLDLSLYVASENYSKTRTAYSSILQWPTQYFVPPQRRTIAKARTEHMGLSSLDLDTLDEERRSSRKAADLIPQSLRTSRPTVTGLMKEKQSSARFKLDALVDAAFKPLEQLLGKKKWMLSDERASSLDCVALGYLSLAFIPEMPLSWLAEGLRGRYPDLCKYVEDGVKEVFGGKVTVEDALLSSTGEQSAMIQGENESGLPWRAPEQNLSSAGASILNSTFDSVPFYKRTIVTTSSTQPQHSTDAPPHSLTSTLLPPLLASAGAVAAIASYLLYSSLSTQEPEKRNLSDMGEAGAMFAGLDFGEPNKEVRVPPAEGRVPVGVEVDIEGVGERRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNX5</accession>
    <name>A0A8H6CNX5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155317.1">
        <property type="nucleotide sequence ID" value="XM_037299316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNX5"/>
    <dbReference type="GeneID" id="59336846"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd22117">
        <property type="entry name" value="F-box_FBXL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045048">
        <property type="entry name" value="FBXO31/39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706">
        <property type="entry name" value="F-BOX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706:SF130">
        <property type="entry name" value="F-BOX ONLY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12014">
        <property type="entry name" value="Cyclin_D1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="50"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="405"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6227009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="553" mass="61713" checksum="27CC91356DC56AFF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPC9</accession>
    <name>A0A8H6CPC9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008327</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155195.1">
        <property type="nucleotide sequence ID" value="XM_037299194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPC9"/>
    <dbReference type="GeneID" id="59336724"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050493">
        <property type="entry name" value="FAD-dep_Monooxygenase_BioMet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789:SF147">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G01950)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="26067" checksum="0FB55B35632D0683" modified="2022-01-19" version="1">MTSARPVEISQLAPLRVKNPSDETDLGNNKSIIRHILQYQIENNRFGRLLGDFLTIGPSAIKVFRNWPRAAKENEEISYDPWVSYHKHTRERILGPKPIFTDDLELDERANIPQRIYRHHRPEFHQLLLAQARKVGIEVEYGQRVIEYLEDAAARKAGGGKLEADLVVAADGAGTKSYILTMGEQVDAKGSGYAIYRTAYPIELALADPQVAEHFKLLENGRSVFELWMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPX7</accession>
    <name>A0A8H6CPX7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008613</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155479.1">
        <property type="nucleotide sequence ID" value="XM_037299478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPX7"/>
    <dbReference type="GeneID" id="59337008"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="168" mass="19157" checksum="9E6343BFADD0F23D" modified="2022-01-19" version="1">MANFMSLPLELQDIVYKYLLTEEVQPTAHKYPHNIPICSSKMIRKEAYKVYLSSNNIIFNNSGTACLWLEHISPYFSTQEEEQPLHLTFDLRHDCNRPAGEKNTQEGDQKRLFHLLGQRTKLDLTLRSGGHLVGDLYRFRAMDLMHGFASATSSPAPAVDVFPSSERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQV7</accession>
    <name>A0A8H6CQV7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WLM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156129.1">
        <property type="nucleotide sequence ID" value="XM_037298793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQV7"/>
    <dbReference type="GeneID" id="59336322"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013536">
        <property type="entry name" value="WLM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053000">
        <property type="entry name" value="WSS1-like_metalloprotease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622:SF1">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08325">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51397">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WLM" evidence="2">
        <location>
            <begin position="10"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="503" mass="55813" checksum="6B724DF8FB9054AE" modified="2022-01-19" version="1">MPIGIQRLNAPRPQPSSRIIFIKPLPGPTSAYALDFLERIAAICHPVMTHAHLSVMTLEEHEPNPEFIGRNFNAGEIIQLVLKAPYTGRWLSFRSVQMVMMHELAHCLQMNHGRDFWKVRNQFAGDLRGLWAKGYTGDGLWGRGQTLLSGEYHTGGRTEDDVSPAHVCGGTFRSSRGGKRKRGGAGSEKKKETYAERQQRRIKKKFGVNGQALGGDEETKVKLERGNKAKGKPRVAGSLRGRELRAAAALARFEEKKEEEKEVKDEEWMLSGSETESDCDDIEVEGQAFELDGSRMRDDKGRGMVKVCEDEDQDDVHVKQEIEELQGLDDLGLISRTTAVRDRTTIERKESRGADRAGTSSSRASATSDIDPPDRIRMEDIPQYVERKDRPSASNTKAVRINIRPLLHADKDTPNTTASSASNPPATAPTETSEPSEMMCPICSMSNGLGSLLCIACSHVLDTSKITRYWRCRRDACEGSQYINAADCGRCGICGSRKDDGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR19</accession>
    <name>A0A8H6CR19_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155946.1">
        <property type="nucleotide sequence ID" value="XM_037298610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR19"/>
    <dbReference type="GeneID" id="59336137"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="210"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="355" mass="38614" checksum="B29BEDA93F204A67" modified="2022-01-19" version="1">MVLTTSYAAPVVPTWLEAYVMLDLFSEDKLVGRLKVAVKQALSLAGGTGNLTQVLGEDLAQTNLTGSGEDTAPPNALVGNVTDSNVAPLGFSHLLVSIILPPSRSRAVSFESIAGAAKVDINDVFLTFHNALLHVARFPAESQMQSFSNCPVLTTLSLAAFKDDPIVGFYAKNVPPDIMHAYQCQQYQRRLQTSSLNGLKVFKVVDDETREIVAVARWQIPYSLSVEQQAEKEKLQEAKLARPEGYNKELEEAFSAALNKLQEKWVDDSKDYVLLGLAVSPSHQRLGLGSLLIRGGLAMADEVGARVYLESSAVGMPLYLKHGFKQVDDILIDMRPYGGTGIASEKCMMREPGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR38</accession>
    <name>A0A8H6CR38_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_007760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155966.1">
        <property type="nucleotide sequence ID" value="XM_037298630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR38"/>
    <dbReference type="GeneID" id="59336157"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF8">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="45"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="338" mass="37597" checksum="65D870A6EAAEA3A5" modified="2022-01-19" version="1">MTSDQTGSRVAAVIACYIVPIPLEIIATSLRIYAKQRQAQGGLDRFTIDDFFIVFATICAVSECCVGLAYGPPHGFGKHIDQVSAHDFETFEVGNYIFSHFYNVTLGMIKLSILTFYYRVFVTPVFRKIVLATITFIALWVLTITIVLALECRPIAKFWNPTIPGKCFNLVAFSYFTNISNLVTDLWIFLLPIPVIWRLHITNHQRLGLCGVFLIGLGTCIISSVRISVVVAQGSTDFTWVGVPLGIMSVFEPLGGILSANLPITYVLFANSFRKVRNTVSGTLSGHLKPRGLLSQSSNNRFGRGQDVEDRWIQLDPQTDWSTESKPLACATRETEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSF7</accession>
    <name>A0A8H6CSF7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156325.1">
        <property type="nucleotide sequence ID" value="XM_037298989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSF7"/>
    <dbReference type="GeneID" id="59336518"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="374" mass="41581" checksum="BAC5AE03B43C8E63" modified="2022-01-19" version="1">MSDRLAPKPTAVVGPSSGASVPTTTLSSDDMPVFGGSKMSIPIFIIAFASYNFFEFRGRSLNREEVLGIVCVCYGRREPPDNNYSLYKRIENPDQSLNKKLDELTDSNKDYLRKQVSDLFQTYLERNPVARFGADELDGFQGRMTSLPWGHKDWAQSKPGLNLQTLRDEQLYLIHAIMHCCVDAGDNRITEFDTAQIVCRHYDYDTSPAIIAKSSVFKNLANPEHKLCQMVAHFINERRHKTFFEAKAHFLYDKYSRIGVLRFRTIIEGEIPSNAVPAATRELAKLSVGVTAGPSQARAPDTPQRTQDLFSQYTHLNSPEIQSPEASSPPDESTSHEATGKGKGRSLESSSSPSGEEVSGKGKGKGKVAKKPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSQ0</accession>
    <name>A0A8H6CSQ0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006944</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156475.1">
        <property type="nucleotide sequence ID" value="XM_037297840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSQ0"/>
    <dbReference type="GeneID" id="59335344"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034916387" evidence="2">
        <location>
            <begin position="17"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="153" mass="16162" checksum="8AB0D236C30728B5" modified="2022-01-19" version="1">MNSILVAVLLASTALSSPPRLHPVPDTTNTTIHTRQVPTGMGSDSGVLISTFTNANCKGDGVSNVDMIYDIEVAQQLRSYSLSAEVGSDDILSVWADMDWDPTGSKPVDPSLNGNTNAACAQFVYDLEDADTTKGCHTLPNVVGCMIMSVAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CT19</accession>
    <name>A0A8H6CT19_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006960</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as a component of both the DNA-binding general transcription initiation factor complex TFIID and the transcription coactivator SAGA complex. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA acts as a general cofactor required for essentially all RNA polymerase II transcription. At the promoters, SAGA is required for transcription pre-initiation complex (PIC) recruitment. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (via core/TAF module) and promoter selectivity, interaction with transcription activators (via Tra1/SPT module), and chromatin modification through histone acetylation (via HAT module) and deubiquitination (via DUB module). SAGA preferentially acetylates histones H3 (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac) and H2B and deubiquitinates histone H2B. SAGA interacts with DNA via upstream activating sequences (UASs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the TAF10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156491.1">
        <property type="nucleotide sequence ID" value="XM_037297856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CT19"/>
    <dbReference type="GeneID" id="59335360"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003923">
        <property type="entry name" value="TAF10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21242">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21242:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03540">
        <property type="entry name" value="TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017246">
        <property type="entry name" value="TFIID_TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="137"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6228849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="222" mass="22617" checksum="0CA217CEA852DCB0" modified="2022-01-19" version="1">MASPNPNPNPTSAPDPSTTQQPSITDDAELDPDIDMAIDPAPPDPENPALDEALPEPREATRKDISLRDFLGKMDDYAPIIPDAVTQHHLLLAGLSPTTTPLPLARLLALATQKFIADIAADAYQYSRMRSSATGAGQSTTAAAIPGAEGGAGQQAGAGKAGGGGKDDKGKSGQHVLGVQRAGYGGGGGGGGQAGRAVLTMEDLGCAVGEYGVNVKRGEFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F695</accession>
    <name>A0A8H6F695_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006933</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000029">
        <property type="protein sequence ID" value="KAF6217417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146852.1">
        <property type="nucleotide sequence ID" value="XM_037297829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F695"/>
    <dbReference type="GeneID" id="59335333"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00488">
        <property type="entry name" value="PCD_DCoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6217417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="125" mass="13936" checksum="29B9C93A58987C99" modified="2022-01-19" version="1">MAGIVQPRFSEGEDGNRLMAETAALLESKWALDETQQGLEKTFNFPTYAKALDFILLIGVGTKLYNHHPDLRNSYKSVFYHWTTHKPRGLSSKDIHMAQLCDDKANLLGHSTGSETLQKSPTSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F836</accession>
    <name>A0A8H6F836_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005815</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147901.1">
        <property type="nucleotide sequence ID" value="XM_037296720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F836"/>
    <dbReference type="GeneID" id="59334220"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034149928" evidence="2">
        <location>
            <begin position="20"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="75" mass="8182" checksum="50A79791059F2D90" modified="2022-01-19" version="1">MQPQKILSLMALFAIGAQALPPLPKANSMTVRTDADEAWEGGTEKRTDADEAWKGGTEKRTDADEAWDGKLQPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F900</accession>
    <name>A0A8H6F900_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dephospho-CoA kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005593</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148484.1">
        <property type="nucleotide sequence ID" value="XM_037296502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F900"/>
    <dbReference type="GeneID" id="59333999"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00152">
        <property type="entry name" value="dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="29693" checksum="8BA90041A5B6FD08" modified="2022-01-19" version="1">MILIGLTGSLATGKSTVSTLLRSPPHHLPTIDADALAHRAILPGTRGYTLILSHFGPTTPDLLQPLDPSAPLGVGANATRPIDRAALGRRVFGDGDAQRRDRAALNAIVHPLVRLAMAREVLGHFVRGRWAVVVDVPLLYESGLDVWASVVIMVAVSSPAVQMRRLRARDAGLSATEAEDRVGSQMGVGEKVGRTRARGPRRGKVVLNDGDGGELEREVGRVMGEVRGEGGGRAWGWWLMGSPYGVAGVSAWEVYQGWRARRRWEEERAGEKARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F925</accession>
    <name>A0A8H6F925_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003969</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148936.1">
        <property type="nucleotide sequence ID" value="XM_037294889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F925"/>
    <dbReference type="GeneID" id="59332379"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="720" mass="79919" checksum="F578D2433D6B5992" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F994</accession>
    <name>A0A8H6F994_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003904</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148873.1">
        <property type="nucleotide sequence ID" value="XM_037294826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F994"/>
    <dbReference type="GeneID" id="59332315"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043041">
        <property type="term" value="P:amino acid activation for nonribosomal peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05918">
        <property type="entry name" value="A_NRPS_SidN3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.980:FF:000001">
        <property type="entry name" value="Non-ribosomal peptide synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000015">
        <property type="entry name" value="Nonribosomal peptide synthase SidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000005">
        <property type="entry name" value="Nonribosomal peptide synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000014">
        <property type="entry name" value="Nonribosomal peptide synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.38.10">
        <property type="entry name" value="Luciferase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45527:SF1">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45527">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="4">
        <location>
            <begin position="582"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1106" mass="122491" checksum="6C71E7782EC9746D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9W3</accession>
    <name>A0A8H6F9W3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003849</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149459.1">
        <property type="nucleotide sequence ID" value="XM_037294771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9W3"/>
    <dbReference type="GeneID" id="59332260"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="203" mass="21619" checksum="E8B83CCA1D24AB46" modified="2022-01-19" version="1">MLATTSPLSSRSTTYYGLILATDSGCSPDQTTQVLNAIIDMRSPCNSAIYTLEPPSNSLSSHFFEPSDFSSASAVFNAGLFATRPLQDLPTDAFPSHDQVQLFCANDTDATCNSLSQGDDKSSETTTWGYIGANPVAAQIYACPALLNSTLPRDAPSCTGTPGQATLGWAFLRTFVQLKTLQTVGKLDSSEIIADQHRGYAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA31</accession>
    <name>A0A8H6FA31_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150210.1">
        <property type="nucleotide sequence ID" value="XM_037293381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA31"/>
    <dbReference type="GeneID" id="59330868"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43611">
        <property type="entry name" value="ALPHA-D-GLUCOSE 1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43611:SF3">
        <property type="entry name" value="FLAVIN MONONUCLEOTIDE HYDROLASE 1, CHLOROPLATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="570" mass="63584" checksum="22B49A03940A5C9B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAK0</accession>
    <name>A0A8H6FAK0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002483</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150238.1">
        <property type="nucleotide sequence ID" value="XM_037293409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAK0"/>
    <dbReference type="GeneID" id="59330896"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="373" mass="42332" checksum="D3C29DA49122B5F2" modified="2022-01-19" version="1">MSLNFTGINVTATVDQAEDTCTLSAFFNKTEPYASGYTYHGILLIVSAVTTTLCLIFSVSLASVHLSNWVKPREQKQLVRIIIFPPISGIFSLFALWFYDASWVLEPFPELYECFALVAMFYLLVLYVAPHESHRDHFFMNLQRFGVVKNKPKLKHDRGSLRWFKMIWVMVFQILPVKLVLNILVWVLSGVTCPVKYKLSSTGTALSVIESIATSICVLGIVWFARRMAPELKQYKVVYKLFSFKGIVGVTLTQGPVFQLFAQYQWFKRTQYVSVLDFAVGTPAFMTCVEMFIASVVFLWSFSAEEYLELSRELPRTRSLGGALVDVLDVRDIFHGIWYMCKISFCCGGRSRAVGKEVGGTESDLESDTVEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAQ3</accession>
    <name>A0A8H6FAQ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Threonine/serine exporter-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002035</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThrE exporter (TC 2.A.79) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150616.1">
        <property type="nucleotide sequence ID" value="XM_037292963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAQ3"/>
    <dbReference type="GeneID" id="59330449"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051361">
        <property type="entry name" value="ThrE/Ser_Exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082:SF4">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="590"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="615"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="671"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="705"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="745"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="770"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="794"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="820"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="887"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/serine exporter-like N-terminal" evidence="4">
        <location>
            <begin position="483"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="353"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="922" mass="100888" checksum="707F19D3FE90FD78" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAW8</accession>
    <name>A0A8H6FAW8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150494.1">
        <property type="nucleotide sequence ID" value="XM_037293665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAW8"/>
    <dbReference type="GeneID" id="59331152"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF284">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="42"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="473" mass="52009" checksum="6CF3CCB610407ED9" modified="2022-01-19" version="1">MEERASIRPGLPLPNPTVSYWQDPPSSLSNNRTTPELPTTVDTLIIGSGITGTTLAYRILSQPSPPSVLVLEARTACSGATGRNGGHTKCVAYREVLDNIKDLGEEEAVKIARFGYNCMRNVHAFAREHGIACDSWQGDTVDVIYDQAQWNKAQKAVAELNRVLGDDDPVAQYKFWDAEEAEKRFLAQGSLGAVSYEAGSLSAYKFVIGVLALALEKGLNLQTETPALRIGKYESGHRGWIVETPRGAVKAERVVLATNGYAAHLYPPLQGVIVPLRGHMTAQRPGSGLPKDGLATTYSFIYDDGYEYMIPRPQGSMFAGDIMIGGGATKGRDEGLCEFGTTDDTTTDAVILKYVEDSAAERFGSNWGHDHPEGRLRKAWTGIMGYSSDGFPLIGQLPDENDLYIAASFQGSGMVLCFESARALFKIMSQEDEALDRWFPKVFRITRERMKRKFRGRLHAKVPPMDLEMKSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAX9</accession>
    <name>A0A8H6FAX9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ER membrane protein complex subunit 7 beta-sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001658</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151125.1">
        <property type="nucleotide sequence ID" value="XM_037292586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAX9"/>
    <dbReference type="GeneID" id="59330072"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034742155" description="ER membrane protein complex subunit 7 beta-sandwich domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="21741" checksum="B0C35FEA6E50480D" modified="2022-01-19" version="1">MHFLPAFLLSLLALPTTTLAATTTTTTSLSPPLHTATLHALPVAPTPSKPHPLAILTYSPHHPHLSTLESFTPPANTSDPDALTSVLLYLDGHVGGERYRSSVTATYGFHAPYRGRFRLVLDSKGEAVGASWHSCLSKSAPAAGKGKGKGESGGNARGDFDVLTTKPAPRVVFDKPAKGKGGATAAGAGAGVGGKEGEEEEVVEKTLLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB76</accession>
    <name>A0A8H6FB76_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151487.1">
        <property type="nucleotide sequence ID" value="XM_037292068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB76"/>
    <dbReference type="GeneID" id="59329554"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF3">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="36521" checksum="D31E2C5BD95FDB9A" modified="2022-01-19" version="1">MNPAVIAAVKEKFTAFDTHNITYQVVNGHAITTDILIPKNIQPGKHPIIVSFHGGYLIESAAHACMRITGASLFPGHFLPWIMDFALEHSAVLVRPNYLKLPESTGLEILDNLAQFWKWFRGGLRTAVKSATAGAVEADVSRTLVIGHSAGAYLAVQSAITQPAGTFGAVIAGYGMLDMRSPWFTQRYEKHPLGNPTLPPSLVDDHLAAMEPGQVVSAVTPPVRVDLALATVQYGRYPDMLGDDAVVYPMEMLGSVKTFPPLFIYHGTEDSAVEVRQTEAFVEKLKDVLPGAKVLVRFEHGDHGFDAPVGLEVPWLKEGLEFVTGEWLRRVDVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBE5</accession>
    <name>A0A8H6FBE5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001565</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151034.1">
        <property type="nucleotide sequence ID" value="XM_037292495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBE5"/>
    <dbReference type="GeneID" id="59329981"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029167">
        <property type="entry name" value="Mug117"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15474">
        <property type="entry name" value="MU117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="167" mass="18478" checksum="B7C24C2F4A90CEFC" modified="2022-01-19" version="1">MAKYFGCNPNIGEYMSLTNADNWALWISTSSNCNGHDFLCGSSVSKGDCQTAASRYGNDTMYSQYTSHWFNSEYWEGCTAIFQCANEYPAGGISGAEIQDYFAEIYTSTAEGGAGCGICGSVYLKNGCEFTFNECDYLSYFEEELADENDIIPLDQITPQTSRPRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBK7</accession>
    <name>A0A8H6FBK7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151815.1">
        <property type="nucleotide sequence ID" value="XM_037292396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBK7"/>
    <dbReference type="GeneID" id="59329882"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034149767" evidence="2">
        <location>
            <begin position="25"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="136" mass="12470" checksum="64EBFC406A0EDD08" modified="2022-01-19" version="1">MHLRTSTSAILLCCLLSLFAGASAELIPRNIDLIPRQASASSSPSATAAASAAASAAFTAAASGVAQGGGENNGAGGGQGGGEGGASGGGSNPVIAAGSMGVTSASSKAGAAPRATGEVNARALAMGAAGLIVGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBU2</accession>
    <name>A0A8H6FBU2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001194</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151543.1">
        <property type="nucleotide sequence ID" value="XM_037292124.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59329610"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16169">
        <property type="entry name" value="Tau138_eWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046488">
        <property type="entry name" value="Sfc3/Tfc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044210">
        <property type="entry name" value="Tfc3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035625">
        <property type="entry name" value="Tfc3-like_eWH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007309">
        <property type="entry name" value="TFIIIC_Bblock-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04182">
        <property type="entry name" value="B-block_TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20222">
        <property type="entry name" value="DUF6581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="B-block binding subunit of TFIIIC" evidence="7">
        <location>
            <begin position="186"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor tau subunit sfc3/Tfc3 C-terminal" evidence="8">
        <location>
            <begin position="1887"/>
            <end position="2296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="262"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="504"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="569"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="726"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1013"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1325"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1411"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1621"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2341"/>
            <end position="2379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="509"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="726"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1042"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1092"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1133"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1166"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1330"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1629"/>
            <end position="1643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2345"/>
            <end position="2374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6222108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2393" mass="266603" checksum="0BE2AD1961C7457A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCC0</accession>
    <name>A0A8H6FCC0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152439.1">
        <property type="nucleotide sequence ID" value="XM_037291004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCC0"/>
    <dbReference type="GeneID" id="59328483"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034712164" evidence="1">
        <location>
            <begin position="17"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="22384" checksum="30BA6CC32EDB3FCB" modified="2022-01-19" version="1">MCLLLLLLQSFYGAQAAPAAVDPASLSLNTIIAPNLRTIPSLSSEESINALALFLNTSNPAGIVLYRVPDTSTLLRIGLQDNPIQKPNIGRTILRTQQSLAAFIKAYDADDTPLARLDDPYESDIRYSGCFFGVATFPIGYHHLTYGIVMNVLQGLWLYLYRAGRFQMAAFEVRDDQFGTVGVGKITPDRPDGLVLGMVGAGNGSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDA7</accession>
    <name>A0A8H6FDA7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">5-aminolevulinate synthase</fullName>
            <ecNumber evidence="11">2.3.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">5-aminolevulinic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Delta-ALA synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Delta-aminolevulinate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_000481</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the synthesis of 5-aminolevulinate (ALA) from succinyl-CoA and glycine, the first and rate-limiting step in heme biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>succinyl-CoA + glycine + H(+) = 5-aminolevulinate + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="2.3.1.37"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.37" evidence="11"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152855.1">
        <property type="nucleotide sequence ID" value="XM_037291420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDA7"/>
    <dbReference type="GeneID" id="59328900"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00375"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003870">
        <property type="term" value="F:5-aminolevulinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000006">
        <property type="entry name" value="5-aminolevulinate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010961">
        <property type="entry name" value="4pyrrol_synth_NH2levulA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01821">
        <property type="entry name" value="5aminolev_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF102">
        <property type="entry name" value="2-AMINO-3-KETOBUTYRATE COENZYME A LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="13">
        <location>
            <begin position="181"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU910713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6223638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="614" mass="66388" checksum="936621585E055E11" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDR9</accession>
    <name>A0A8H6FDR9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010692</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153178.1">
        <property type="nucleotide sequence ID" value="XM_037301544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDR9"/>
    <dbReference type="GeneID" id="59339082"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="479" mass="53835" checksum="D2585AC6A932E2A6" modified="2022-01-19" version="1">MRTANNSEMDRIPPPPYTEVADASDAASQPVQASLRGGYMRPSLPSEMPSDENNLSSAITYFEDPDDPDLHRAGVYLSLAEYTITFNRETTRDDLAYPLPIETYIARDVTSLDWSTFVNFLFPMRDEVRHGKLRPEKDPQRHSFIEEDTPARRDRILAVIAEWNENFFNPRRIHIKAGFSPLPSYPSSRSTAIPSVAQGPYVESRPLQPAQPTMYRDAPAQWSANLSTPQPIHRSLSTSSSDSSSSSSMDSVKSKDLEGVDLGQIRSALLAFQLDATKKDHLHASVRQLRDGFRSQRRNLSGKDSKELKKEYRNQRKEIKKEVKAVVKEVKATRKADRKVRKAERKSQREGKRAERGGNDRIKDSEDTGRRAEERAAEKVRHAQERGREAEGRASEKAARAHGRAREAQAQEATAVARAQERVADARARGWDGEAAATQRAQEIKARTGAAQRRARETAGRDRYEINGGQETGVLLTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEK2</accession>
    <name>A0A8H6FEK2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010383</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154053.1">
        <property type="nucleotide sequence ID" value="XM_037301236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEK2"/>
    <dbReference type="GeneID" id="59338774"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008729">
        <property type="entry name" value="PA_de_COase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40087">
        <property type="entry name" value="PHENOLIC ACID DECARBOXYLASE PADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40087:SF1">
        <property type="entry name" value="PHENOLIC ACID DECARBOXYLASE PADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="7">
        <location>
            <begin position="260"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6225186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="381" mass="42042" checksum="87468E0A6806E20C" modified="2022-01-19" version="1">MTAPATDFPFAKDLKDLHLVYDYNAHDAQGKPEKWRYEIWFFSEDRVVYKIHGGPMAGRTNFQTCSFQCIRPGELWQCNWLEAIPAETGTVVSLVYDIPKKRITTLLAFSKGIVIAGGTLPLDVGDQIADGGDCKVTGRMRKRHTGIKETRRISRGGAVWRALAFRRTGICSRNRLILWISSGARSHPKSGDFGWEILTFSSSSSGAKRNARRDNHSKTSDDIPFPRPQRRPPSFPSLKALQMFPRLRTPILQNTGSTARDHLASERTFLAWLRTGLGFVALGIAIERFGQLDPVPTPAAKGKGGHPKKDQSEGLVRGLLATGSGSIAYGTARYFSNMRMLEKGSYKPSLYGAGALSLAVVGLTGAACWEIVRGRREEGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEM9</accession>
    <name>A0A8H6FEM9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154284.1">
        <property type="nucleotide sequence ID" value="XM_037300436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEM9"/>
    <dbReference type="GeneID" id="59337970"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="151" mass="17451" checksum="C6E5540D34B55FE0" modified="2022-01-19" version="1">MAFNSPIPGFSAEQVRSITKLIEDVLDKALDKHFGPLPPHQKQSVIMPKPAQPPKQEQEHRPLQQANVEKMRRKLWRRIRARTAAVLFKRAHSPQFHTRPLCRIAEILPLASAVLHFLPRFSPLFSPLNSPLFLLSPLRSATFDSRRIWRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF34</accession>
    <name>A0A8H6FF34_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154252.1">
        <property type="nucleotide sequence ID" value="XM_037300404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF34"/>
    <dbReference type="GeneID" id="59337938"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="330" mass="38172" checksum="3449A13D80D02C06" modified="2022-01-19" version="1">MDPYIDTIPDDHGEEIKKRVRERDDPDNEGRPTKRARANTSALLPPEGDRQDIEEPLKPEDDLDKYDHERLQQGLRYIDGAAKRKITPRTQEFLKLHKDAYKVALDGEVPDKASRAVVQAMDKVYFSAAFYLKPRSSEEHEHLMYYVKLRRLEFLANYGDYLGQELQIIRADLNAAAIGVDSEIQKAALELGPSKTWITIANELAGTDVADLRKHVTIACAVLGIDSEHMLWLIKEWAERNRVFRSQIRQYISDCHWNRLAEQICRDLTELLNVAPDKDTAAKYEKVLLSIQNEYFDAISRDDPQLWLLNEKSEKLTQEKLGREKKRAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFL5</accession>
    <name>A0A8H6FFL5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein CSN12 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009401</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155088.1">
        <property type="nucleotide sequence ID" value="XM_037300262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFL5"/>
    <dbReference type="GeneID" id="59337796"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000366">
        <property type="entry name" value="COP9 signalosome complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="252"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="463" mass="51426" checksum="F58DE2B7CE52F7B5" modified="2022-01-19" version="1">MEVLCREYWHANQEGSGPLLAATISPIAPPNYLNRLDAIYGDTNAASISRDISYSLTKTANAAIQYPNPEINSWTEIYSAYWYAIGAILAAEHPTTKPDWTKVYEAWKDVANTVIKGYSGGVLEAWTLPVLYTAGKHLRAFAIKADENARASGGDTSFNAGGLQEDIAGDFGKNEKLEDAARVINRMFTLCISDRAPLEESRKWGLYYTTNLLFKTYFKLNSIGLSKNILRALSASKTDMPTLEAFPRSHIVTFKYYVGVIQFLEEDYAQAEENLTSAWHMCHRSATRNIELILTYLIPTLLLTRQQLPSSRLLSPYPSLGSLFAPLTLAIRIGSLSAFDTALAEGEHEFVKRRIYLTLERGRDICLRNLLRKVYLAGDLDEEGKRNTRIYVKDFAAAIRLGESGASGEGMDVEMDGSAGENNDEVECLVANMIYKNLMKGYISRERGIVVLSKNGAFPGTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFU0</accession>
    <name>A0A8H6FFU0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fucose-specific lectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154847.1">
        <property type="nucleotide sequence ID" value="XM_037300021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFU0"/>
    <dbReference type="GeneID" id="59337555"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.70">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89372">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="54970" checksum="F785C1EC9503A5B0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFW4</accession>
    <name>A0A8H6FFW4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mediator of RNA polymerase II transcription subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Mediator complex subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">MED8</name>
        <name evidence="4" type="ORF">HO133_008473</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mediator complex subunit 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFW4"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019364">
        <property type="entry name" value="Mediatior_Med8_fun/met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35043:SF8">
        <property type="entry name" value="DUF4220 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35043">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10232">
        <property type="entry name" value="Med8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="484"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="487"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="541" mass="61013" checksum="F14FDC07353A91C4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH85</accession>
    <name>A0A8H6FH85_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155985.1">
        <property type="nucleotide sequence ID" value="XM_037298649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH85"/>
    <dbReference type="GeneID" id="59336176"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="73" mass="7763" checksum="29AB79AAA9E59CEF" modified="2022-01-19" version="1">MKRTLNLQTTTTITILVDSSDEEIVDGPSSLPLGNAMTNGGAQSETIGSEDFIPDEDKDEDESGEVAYVRNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHB4</accession>
    <name>A0A8H6FHB4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156209.1">
        <property type="nucleotide sequence ID" value="XM_037298873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHB4"/>
    <dbReference type="GeneID" id="59336402"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052965">
        <property type="entry name" value="Pigment-catalase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694">
        <property type="entry name" value="DESICCATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694:SF26">
        <property type="entry name" value="OS05G0151100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13668">
        <property type="entry name" value="Ferritin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034864911" evidence="1">
        <location>
            <begin position="20"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="306" mass="32149" checksum="2AB9F2330E01CE2A" modified="2022-01-19" version="1">MKLFTTSAVATLLAITAIAHPLKRQAAVTDVDILQYALTLEHLENTFYKGAISSMSEADFMAAGFSSAYYNNLKYITHDEEQHVELLSSALMAAGQKPVAACTYDFPYTDPKSFVTLASVLEGVGTSAYLGGAGLITSKAYLGVAGSILVTESLHTSVQRFNLGEVAPANPYGTALGLNPVYTLAAAFITSCPSTNAALPVMAFPTLTATQGIPAAENMTFTFSVKGTLPTTFYVVFVSGLSTTSVMPTMQGGMIAAVIPPTAMGQTYVFISKSMTNGTITDAMILYGPAVIEVTPPAPTFDLSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHG0</accession>
    <name>A0A8H6FHG0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156359.1">
        <property type="nucleotide sequence ID" value="XM_037299023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHG0"/>
    <dbReference type="GeneID" id="59336552"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="213" mass="23036" checksum="C494F84733531808" modified="2022-01-19" version="1">MNVSAVLAFNCLSIVYCRVYEAIYMFFNNLFFSFLLPVLSAAALFPASDNANPDHPSPVQHVERDLSQSPTLSNITLSTPSSLNTTNAIDPHCFLPNPRQPHIQPSDCHTALYALVVSPSAMLLEHWDRSSILPVESASGTCSIILARVTDTADGIFQPILVAHAAALLVRSCVTARWGYQGGTARIGQRQDFVVYLSASWAAGDMGIARATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHP6</accession>
    <name>A0A8H6FHP6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hepatocellular carcinoma-associated antigen 59-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007959</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TLS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156163.1">
        <property type="nucleotide sequence ID" value="XM_037298827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHP6"/>
    <dbReference type="GeneID" id="59336356"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010756">
        <property type="entry name" value="Tls1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486:SF2">
        <property type="entry name" value="SPLICING FACTOR C9ORF78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486">
        <property type="entry name" value="TELOMERE LENGTH AND SILENCING PROTEIN 1 TLS1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07052">
        <property type="entry name" value="Hep_59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="267"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="39069" checksum="71561F704E51C604" modified="2022-01-19" version="1">MQTSLPSEGENSSDLFRPSKRRKFYRKRTDGEDEGSPTHSSAPPPESMTVDELISHQGRLTETQHHKTDEEPLSIAAILRQRKLALPRKGGIEFTNSHTSTSSISQTSDALIVKEGNTPADIKSVIERFAPQTGQVSDVADKHMMAYIDSELAKRQRQNQSTSTQSLNGQELSPNGALLDAGLHRQPAALGKLHEIDLGPDAKLRNIALTEAAQRRLEGIEPEIEESTGKVRLRRDGKPWRPRKRRNSEDVKRDKLVEEVMRESRLDIYDEPDEEVNNDDQAADDRIAEQFRREFMDAISSRQQKAAQAKKPVKGKEVDDQPRGPKLGGSRSARAAMREREEAAKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI08</accession>
    <name>A0A8H6FI08_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156485.1">
        <property type="nucleotide sequence ID" value="XM_037297850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI08"/>
    <dbReference type="GeneID" id="59335354"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="226" mass="25101" checksum="6873A95CDA5FED57" modified="2022-01-19" version="1">MIEMVCTNRPEGFGPLSHLYSHMLTSCFLDTILVPLCTWVYLLALVILAVTGTRLSTRASQQSSRPSDVSHYPVHKTTEDVEIVRLETGGRTRRHAKTRMAFSILFYLLIFAQIVMCILEIVRLSYADRGIGLLPFTFVTLVGAAMVHYTQGLEGMIFGWKYLNLAVWIALAVTNGVKVAEEIKEGTGATKGSKYPVSDEMTDVSVMIGVYGMLAILEGFLGVWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI81</accession>
    <name>A0A8H6FI81_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Queuosine 5'-phosphate N-glycosylase/hydrolase</fullName>
            <ecNumber evidence="6">3.2.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 6">Queuosine-nucleotide N-glycosylase/hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007101</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the hydrolysis of queuosine 5'-phosphate, releasing the nucleobase queuine (q). Is required for salvage of queuine from exogenous queuosine (Q) that is imported and then converted to queuosine 5'-phosphate intracellularly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>queuosine 5'-phosphate + H2O = queuine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:75387"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17433"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:194371"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5 6">
            <dbReference type="Rhea" id="RHEA:75388"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the QNG1 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156629.1">
        <property type="nucleotide sequence ID" value="XM_037297994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI81"/>
    <dbReference type="GeneID" id="59335501"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019438">
        <property type="entry name" value="Q_salvage"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21314:SF0">
        <property type="entry name" value="QUEUOSINE 5'-PHOSPHATE N-GLYCOSYLASE_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21314">
        <property type="entry name" value="QUEUOSINE 5'-PHOSPHATE N-GLYCOSYLASE_HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10343">
        <property type="entry name" value="Q_salvage"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00035119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="366" mass="42300" checksum="7EB34E2F311735D3" modified="2022-01-19" version="1">MSDDEVDSDLLELLRKSLGIDGNSSPRPAKTGVLEDAEYIYDNSTDVALDMRGTKAAASSIWTLIQEKQYSTRDWSKHELHPKAKDESTVDFIFLMDLLNFCFWSDGALPEQYAVEYRGKTWTGYWSLVAAIQRALDEGIPITSPSFWINEGKCTDALLQHVFRTSANSPPMPLLGDRIYCLREAGYVLRDQFEGTFTTCIEQCKGSAAALVNLIVDNFPCLDDRHRFEDTTVRFHKRTQILVADLWACFEGEGFGYFHDIDQITMFADYRIPQMLHSLGCLQYSPPLESHIRQRRAIESGHPWEIQLRGCSIWCIELLRREIRRQHPETHLNAILIDFFLYDTMKEMETKGHDCIPHHRTRSIWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIF4</accession>
    <name>A0A8H6FIF4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Transcription initiation factor IIA subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_007201</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">TFIIA is a heterodimer composed of the large TOA1 and the small TOA2 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIA subunit 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156729.1">
        <property type="nucleotide sequence ID" value="XM_037298094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIF4"/>
    <dbReference type="GeneID" id="59335601"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10145">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.190:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000003">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.190">
        <property type="entry name" value="Transcription factor IIA gamma subunit, alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009083">
        <property type="entry name" value="TFIIA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015872">
        <property type="entry name" value="TFIIA_gsu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02268">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009415">
        <property type="entry name" value="Hum_TFIIA_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit N-terminal" evidence="10">
        <location>
            <begin position="8"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="11">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6229087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="113" mass="12770" checksum="95E0F2C7DAA7DC53" modified="2022-01-19" version="1">MASAANSYYELYRGSSIGMSLIDTLDDLVHSGRIEPQTAMKVIATFDKSITEVLADKVKARMNFKGHLDTYRFCDEVWTFLIKDVNFKLDNQNNVTAEKIKIVSCNSKKPGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIQ9</accession>
    <name>A0A8H6FIQ9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007383</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156909.1">
        <property type="nucleotide sequence ID" value="XM_037298274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIQ9"/>
    <dbReference type="GeneID" id="59335782"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF3">
        <property type="entry name" value="DUF DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G11150)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="6">
        <location>
            <begin position="48"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="161" mass="17562" checksum="201CF43E39DDFA15" modified="2022-01-19" version="1">MPSVAQALGPLSTQAIIIDHSSTSPGHLFLSRPIFAPLLFPNESSDARDHCANERTFLSWLRLSIYMSVVSVAIVISFHLKSQPTSIEKRIALPVGLIFWFLSVACLASGFANYVKTVTKYSRRQALVQSGWKTQVVFTIVSCAIVAACVLFLSTNAASSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJQ7</accession>
    <name>A0A8H6FJQ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RlpA-like protein double-psi beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004176</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157096.1">
        <property type="nucleotide sequence ID" value="XM_037295095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJQ7"/>
    <dbReference type="GeneID" id="59332585"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF28">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034657461" description="RlpA-like protein double-psi beta-barrel domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="RlpA-like protein double-psi beta-barrel" evidence="3">
        <location>
            <begin position="31"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6229839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="170" mass="18124" checksum="55AA9FCCE26C88D0" modified="2022-01-19" version="1">MPRHSSILTWTLLSICSFTTQAQTVYTGDGTFYSPSVGLGSCGQQYQDTDLVAALAEVSMQAYNPGNPNNNPLCGYKVNVWNNANPSTTVTVAIVDTCPGCQGQYDLDLSPAAFNQLADPSVGRIQISWEFAETVSLTTGPFAGAVPGERVAINQKVISNPEVAESRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK11</accession>
    <name>A0A8H6FK11_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004313</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157232.1">
        <property type="nucleotide sequence ID" value="XM_037295231.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332721"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018791">
        <property type="entry name" value="UV_resistance/autophagy_Atg14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10186">
        <property type="entry name" value="ATG14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="126"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="183"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="302"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="444"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="547"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="672"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="743"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="934"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1145"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1249"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1412"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="685"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="743"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="946"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1152"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1264"/>
            <end position="1275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1441" mass="160602" checksum="C3476FAB9B04E67E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK69</accession>
    <name>A0A8H6FK69_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoglycerate mutase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157274.1">
        <property type="nucleotide sequence ID" value="XM_037295273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK69"/>
    <dbReference type="GeneID" id="59332763"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF32">
        <property type="entry name" value="ANCHORED PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G10590)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034031457" description="Phosphoglycerate mutase-like protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="39425" checksum="B20B3A704B13AF88" modified="2022-01-19" version="1">MLSSVLLWVLAASGLFSLSVADSSKTPSSYINYTTVTGYFLQDEPSTNASTFNFMTTNFGLINRTYPADTTLHSHHNLTQWQRFKHQVTQLNREAPRGTEYKLLYMGRHGDGYHNDAQAYYGTPAWNCYYSELDGNSTVTWSDAHLSALGVSQALAVNEFWASEIKDQKIPTPQSYYTSPLTRCLQTANYTFNGLDLPARHPFIPLVKEYFREGISGHTCDRRSNETYIHDLFPSYTIEPGFTQDDELWVALHGETSTDQAVRSKRVLDSVFSTDGSTYISITSHSGEIGSILSVLGHQAFGLSTGAAIPVLVKAQTIGGASATTALQPYTALCTCTSPPAVTATTSVCAYPPAETSPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK92</accession>
    <name>A0A8H6FK92_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_004383</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157302.1">
        <property type="nucleotide sequence ID" value="XM_037295301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK92"/>
    <dbReference type="GeneID" id="59332791"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="225"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="34"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="603"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="664"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="932"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="993"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="603"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="675"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1017"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6230045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1026" mass="113912" checksum="FBE02AF5F908E4E6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKB8</accession>
    <name>A0A8H6FKB8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157324.1">
        <property type="nucleotide sequence ID" value="XM_037295323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKB8"/>
    <dbReference type="GeneID" id="59332814"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="60"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="271" mass="30462" checksum="FDFFF72460352B4A" modified="2022-01-19" version="1">MQSILDIDILPAQHSSVSTMAHVFINSFDLDGSVKLMYTKDEIVPIIQEILHDYMDDDAIQFRLAVARDTRRLAGWMSFGVIPALGRVPEFAFNEMTSWAAQKLIHGYASDYRYPLAAQLEDRSRHGQSLHMLRHRLVINTIVTDPEYRRLGVAGKLLRCAVDRARSNDWAIWVQTPTVYEGLFWRNGFHDVGTFGLDLNAFKPPEEVAMGVHGRQLGIQTWRQMKLGSRAESLLERAIAETAEAEGTRGHVPNPRSTSPPSSTSANTSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKF7</accession>
    <name>A0A8H6FKF7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA exonuclease 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004504</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">3' to 5' exoribonuclease required for proper 3' end maturation of MRP RNA and of the U5L snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157422.1">
        <property type="nucleotide sequence ID" value="XM_037295421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKF7"/>
    <dbReference type="GeneID" id="59332912"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000109">
        <property type="entry name" value="RNA exonuclease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF112">
        <property type="entry name" value="RNA EXONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="13">
        <location>
            <begin position="381"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="46"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6230165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="591" mass="65312" checksum="7423871F5E5ED5A6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKJ3</accession>
    <name>A0A8H6FKJ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pyruvate dehydrogenase protein x component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004565</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157482.1">
        <property type="nucleotide sequence ID" value="XM_037295481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKJ3"/>
    <dbReference type="GeneID" id="59332972"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF82">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE COMPLEX PROTEIN X COMPONENT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="6">
        <location>
            <begin position="138"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="42544" checksum="7C388EAEF2AE2B61" modified="2022-01-19" version="1">MTEGNIACWKVKEGDSFSTGDVLLEIETDKAQMDVEAQDDGRMAKITQEAGSKGVKVGTRIAVLADIDDDLSTLTLPAAESAPTPSEQENKASINASKSSESHASASPSSRAEDSSTKPSNPPAPSTSPGRPQKQLYPLYPSIAQLLHENGLSSSEADKIPASGPKGRLLKGDVLGYLGRISSTYSSEQSTRITKLGHLDLSNVKPAPSKAMALSQPSKAQAQKRPGPELESTDTEVAIPISLSSVISVQQRIQATLGVTLPLSTFIARATELANDDLPRSAATKPTADELFNDVLGLNNVSTKTSKARYVPQITALPTKPFTERAGPRRQQDIYDILTGGSSTTALTKKFGIQPPGMMAGSKTGESTNVFSVSAAKGEEKRARVFLERIVHSKPQLLYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKV8</accession>
    <name>A0A8H6FKV8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157653.1">
        <property type="nucleotide sequence ID" value="XM_037295652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKV8"/>
    <dbReference type="GeneID" id="59333144"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040632">
        <property type="entry name" value="Sulfotransfer_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36978:SF4">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36978">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOTIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17784">
        <property type="entry name" value="Sulfotransfer_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="184" mass="20404" checksum="F5DC6280B25FEAE1" modified="2022-01-19" version="1">MWTVTLGATVSGACGSICDLSAIAFGPELMDAYPDAKTILTNQPVDSWHTSCSKMLLQAKKYWLHGVLQHFAGVTGLVYPLRRKYWQCLFDDDFESNGKAAMCAQYVEVQTHAQEIGSRVLGFGLRDEWGALCELLKVEVLDHPCPRRNEGGDWILKMRERARKAAAVRFFRGGLPVAVLVLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FLK8</accession>
    <name>A0A8H6FLK8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000022</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157836.1">
        <property type="nucleotide sequence ID" value="XM_037290962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FLK8"/>
    <dbReference type="GeneID" id="59328441"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF136">
        <property type="entry name" value="ULP1 PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="315"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="737" mass="80921" checksum="C9DB4045C27311B5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L022</accession>
    <name>A0A8H6L022_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_004847</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the dynactin 150 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157760.1">
        <property type="nucleotide sequence ID" value="XM_037295759.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333253"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000743">
        <property type="term" value="P:nuclear migration involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022157">
        <property type="entry name" value="Dynactin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF6">
        <property type="entry name" value="DYNACTIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12455">
        <property type="entry name" value="Dynactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0243">Dynein</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="14">
        <location>
            <begin position="27"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="79"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1109"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1252"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="278"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1112"/>
            <end position="1133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6230503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1317" mass="147162" checksum="49592E7C070A11FF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C4G0</accession>
    <name>A0A8H6C4G0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000029">
        <property type="protein sequence ID" value="KAF6217412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146847.1">
        <property type="nucleotide sequence ID" value="XM_037297824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C4G0"/>
    <dbReference type="GeneID" id="59335328"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="448" mass="50097" checksum="5348840558671DDC" modified="2022-01-19" version="1">MAILRIPNISERMRAVELFISSYRGATGSTAATMHNMQDIEREMAMSMTATESETIVVTAADAQTLIQSSKEYGEKYTFQVVLPLVKKHTSNIEFIVAFLTGLFRASEADKLRLEIVQNLFQDILGDVISDLQIQYREVGHEQLLNEYAKRRRFDYNGHVSQAAEDQSPRAMTAEKLASLFHSCEKLGLSREIDQLADKIISHAPNANTVTFEGVLLPLIKQLPPPVERASKIANPHSYTRLFRTVLSSYINTYVQPAPKKPTGLERQPRGCSLYCDDCVKLDAFLNNQQRAQVHFSLNGKRRDHIEERLRQSYCSTETIKRRTPYTLVVKKTGMEWENAMKEWKQRCEVAWKTVEEIGIEKLRGLLGEGWEDVVGLGGIMQDGREESGERCPFGNLAQGKGTSGAVETGANKVAGHVGPEIIDLDCELGGGDPALKGVLTNNTMLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6C7</accession>
    <name>A0A8H6C6C7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147054.1">
        <property type="nucleotide sequence ID" value="XM_037297618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6C7"/>
    <dbReference type="GeneID" id="59335122"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00657">
        <property type="entry name" value="Ferritin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052965">
        <property type="entry name" value="Pigment-catalase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694">
        <property type="entry name" value="DESICCATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694:SF26">
        <property type="entry name" value="OS05G0151100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13668">
        <property type="entry name" value="Ferritin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034520108" evidence="1">
        <location>
            <begin position="17"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="316" mass="32350" checksum="93E0B0B93F6AFC04" modified="2022-01-19" version="1">MKTTFAVAAFAALAAASPVSQTGSFLKKIRQTAPAITDADILNYALTLEHLEDTFYQEGLKNFTESDFATAGFDSTFYANLKEVSSDEMTHVAFLTAALEKAGATPVAACTYNFGVTDAKSFIATASILEGVGVSAYLGAAADIMSAAYLTAAGSILTVESRHSSYIRASLGESPFPSPFDVPLTLDEVYTLAAPFIVSCPSSNPTLPVKAFPMLTSSPKNGNITAGSEITLLTPGSVVTAADGSSQIYGAFITVTGPVFAPATAVDGGFTLTVPTAPNGTAPISGQTYVVLTGCNTTVTDDTVAAGPAAFEVGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6F9</accession>
    <name>A0A8H6C6F9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006521</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147129.1">
        <property type="nucleotide sequence ID" value="XM_037297419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6F9"/>
    <dbReference type="GeneID" id="59334922"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF160">
        <property type="entry name" value="SAT4 FAMILY MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="51"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6217694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="247" mass="27286" checksum="456A3E02D5F5AA96" modified="2022-01-19" version="1">MAAAAEALLQGPGLQPPPGVIPNLTDPPSQVRWVYITLPICLAVSTPFVWIRLCTVFFILKSHGWADYASATAWAFLVGYCTSMYFTAEYGGGVHQWDVPLGRVLEFAKLANTTEILYSPAIFLAKLSILLMYRRLFDTAGTGKTHHLIHILIWANLAFYFPYLFASIFQCVPRARIWNPTLKGGCVNLQAAFIAASAINVVSDFSILLLPLYRISKLKLPTRRKIGVLAIFAVGLLYAPLLSHPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6K1</accession>
    <name>A0A8H6C6K1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147370.1">
        <property type="nucleotide sequence ID" value="XM_037297245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6K1"/>
    <dbReference type="GeneID" id="59334748"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990811">
        <property type="term" value="C:MWP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990810">
        <property type="term" value="P:microtubule anchoring at mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052778">
        <property type="entry name" value="Centrosome-WD_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16220">
        <property type="entry name" value="WD REPEAT PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16220:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN WRAP73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="585" mass="64732" checksum="46DD967F2CC6219C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6M5</accession>
    <name>A0A8H6C6M5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147383.1">
        <property type="nucleotide sequence ID" value="XM_037297258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6M5"/>
    <dbReference type="GeneID" id="59334761"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="427" mass="47087" checksum="E2760E3456C1A55C" modified="2022-01-19" version="1">MSKLMRSASTASTSRESSSASLSSTVRPPTVASRTSKNPAPSQIAALTHTLSLPVETPMLSSVVGALGVPLSRSQFSPTPSCFCNAHFWFEQKYIDELTEVITHEIGPQLDKLRIAPRELISRKTERLLSTLQPYQDILTSTTDTSSILSMEPTCPACTLSHFFQNPAAVNALTLAVKGRKHRAHPWPASMAWLDPCPGREWEAKWRSEGKSVAHDRVRVQRWRRETRAHERVPDAPPQVLGHQPGEGCDFCDALRMERADEAELDAAIAEEEEEGDDKETRAVGEPARYSGAADEIHDDDDDDDDASGSETLRSDSSTTLRAMEEESIIKGYLRPPTTTTDSACHPRPDADDLSGNKAEQEARRKSSVMGGYLAHRGGRGEGERAKRASDWARSYQCLVGRSPDSQTTLLFERYSEPGLRAVEGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Z0</accession>
    <name>A0A8H6C6Z0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147537.1">
        <property type="nucleotide sequence ID" value="XM_037296963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Z0"/>
    <dbReference type="GeneID" id="59334465"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="271" mass="28252" checksum="C64439FB2BCBFEAB" modified="2022-01-19" version="1">MTGTNQCGYTTIPSSQWNPSDQICTIGLSASSLRPVKPTTTGQYLFTETDLPTSGSGTTVYGCTSTLTSEAGPVMGTYCAGSKTPLTTLFPPIKTPTGAPAAPSCNSPANPQDASAQGDPDGYVPNPVFFEAAIGVFCGPNCKGKSGCLSATGQTLSSNMDAFKATFFCSDAQSAPGGNKTLDCEPYDWENNPPPYWEASGIMFNLGVKLIDKSCQWTVDSDTCNKWLSNTLTGCPAAGLDWNECVAWTVGAGAAALSLSDWQADHPSGTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Z8</accession>
    <name>A0A8H6C6Z8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006242</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147266.1">
        <property type="nucleotide sequence ID" value="XM_037297141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Z8"/>
    <dbReference type="GeneID" id="59334643"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="15"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6217831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="357" mass="39609" checksum="F4BFDD075D6DA948" modified="2022-01-19" version="1">MGHTEGGLDCLFEASIFLYVPATALPKLAILVSYLRILPNKNIRISVYAVFFITIGYMVACCLAFLLQCHPVARIWDMVIPGECHVISNFLVNGIFNFAIDIMVLLLPVPVLMEWRVSSRMRALMGGVFAIGSLACIVSAVRIYFIARGLNTFRTGIAVIIGIPRSLGVVETNLSIICGCIMVMRPFLRRHLPFLLEIGSQRAEHKHAGVIDDEHPLKVFSITVESRLMKTGAGFGGVNSSAGWIWDQRSRIGTRAIVDTAIQDLDMEGARDWPLRDDLSPKAERADNVIEEIYYASNDPELARKTQTTRTFSRGSQVMDCGSETRGVSKTQARTVSLITAANPCVVRETNWPGFWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C830</accession>
    <name>A0A8H6C830_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U1 small nuclear ribonucleoprotein C</fullName>
            <shortName evidence="9">U1 snRNP C</shortName>
            <shortName evidence="9">U1-C</shortName>
            <shortName evidence="9">U1C</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005683</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the U1 snRNP. The U1 snRNP is composed of the U1 snRNA and the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least 3 U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. SNRPC/U1-C interacts with U1 snRNA and the 5' splice-site region of the pre-mRNA. Interacts (via N-terminus) with TIA1 (via C-terminus); thereby promoting spliceosomal U1 snRNP recruitment to 5' splice sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">U1 snRNP is composed of the 7 core Sm proteins B/B', D1, D2, D3, E, F and G that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least 3 U1 snRNP-specific proteins U1-70K, U1-A and U1-C. U1-C interacts with U1 snRNA and the 5' splice-site region of the pre-mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the U1 small nuclear ribonucleoprotein C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147771.1">
        <property type="nucleotide sequence ID" value="XM_037296590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C830"/>
    <dbReference type="GeneID" id="59334088"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000059">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03153">
        <property type="entry name" value="U1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017340">
        <property type="entry name" value="U1_snRNP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148:SF1">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037969">
        <property type="entry name" value="U1_snRNP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01871">
        <property type="entry name" value="ANNEXINVII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="11">
        <location>
            <begin position="15"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="136"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="136"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6218336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="230" mass="23550" checksum="52E2B19D9A3DC645" modified="2022-01-19" version="1">MDLYLPRLSPSDANSTGDYCDVYLTHDSMSVRKAHNSGRNHERNVLDYYQQIGHEKAQSVIDSITSSYAAEGQAGANPMLAPQGGPPMGGQGFPPPPFGFPGTPPHSRAVAKIPRTTCANRPPSLFTAGMPPPPFMPGPGGPGAPPPGFVPPPGGRGMPPFPPFPPAASPSGNGPPMPGMPGGLPFPPPGGMPPNFQGFPPPMGGPGQGMSPPQGGFPPPQGMPPFQGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8U9</accession>
    <name>A0A8H6C8U9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidate phosphatase APP1 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148570.1">
        <property type="nucleotide sequence ID" value="XM_037295872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8U9"/>
    <dbReference type="GeneID" id="59333366"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019236">
        <property type="entry name" value="APP1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052935">
        <property type="entry name" value="Mg2+_PAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28208:SF1">
        <property type="entry name" value="FILAMENT ORGANIZATION PROTEIN APP1-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G06650)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28208">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE APP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09949">
        <property type="entry name" value="APP1_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidate phosphatase APP1 catalytic" evidence="2">
        <location>
            <begin position="215"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="414" mass="46855" checksum="75FD1121C6220E44" modified="2022-01-19" version="1">MDASMDKGRQVMEETHSERETRSAGDFHKIEELLPKHIGSRWNIPFLDNMGSFLSKFNPLARAVDPEKEEVWLLDNTAYRPVHVYPHQPQPFQAQFTAAYFKRYTGKDWSKAVASIADTLGLADGEGRAQAEKTIAHRLKPFVETIAPARYVDIKLPNGTVEKLGPGGRSAVSQQIIVSIQDHKEGESAEISAISPQVTPHGPMLTHFAEPEGWLMVSDIDDSIKITMTPSPIGILRSTFVSDPTPIAGMPELYAQIATSLNPAWFYLSASPYNLYPFLRPFLHQYYPKGTTILRDASWMDLGGFLASLTQGTEAYKRSRIEEIHNWLPRRKVLCVGDSTQSDPEAYGDICRKHKGWVKAVFIRKVTDVAEMKDTDKNTDQRFEKAFRDVPREIWRTFEDPKELYEAVEQLKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9X5</accession>
    <name>A0A8H6C9X5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_004034</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAF6219565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037294953.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
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        <property type="entry name" value="AreA_GATA"/>
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        <property type="entry name" value="Transcription_factor_GATA"/>
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        <property type="entry name" value="Znf_GATA"/>
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        <property type="entry name" value="Znf_NHR/GATA"/>
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        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GATAZNFINGER"/>
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        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="11">
        <location>
            <begin position="682"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="393"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="733"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="58"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="89"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="176"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="282"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="585"/>
            <end position="596"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="616"/>
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        <location>
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        <location>
            <begin position="752"/>
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        <location>
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        <location>
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        <location>
            <begin position="819"/>
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        <location>
            <begin position="897"/>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="931" mass="99852" checksum="016A538CD66E3D0D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA28</accession>
    <name>A0A8H6CA28_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_003917</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>propanoyl-CoA + oxaloacetate + H2O = (2S,3S)-2-methylcitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23780"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:58853"/>
            <dbReference type="EC" id="2.3.3.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Organic acid metabolism; propanoate degradation.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the citrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
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    <dbReference type="Proteomes" id="UP000593566">
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
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    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
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    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
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    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
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    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007128">
        <property type="entry name" value="PRK09569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF15">
        <property type="entry name" value="CITRATE SYNTHASE 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="474" mass="51836" checksum="D3D5A38A05B4CA4A" modified="2022-01-19" version="1">MLSTTRVAPRAAVLLQPRLLSSIAHSIRTYSSASEPDLKTTLKEAIPAKRELLKKVKSHASKIIGEVKVENTLGGMRGLKAMLWEGSVLDANEGIRFHGKTIEDCQSVLPKGTSGTEMLPEAMFWLLLTGQVPTTTQIRAFSRELAENSTLPNFVEKMLDSFPTDLHPMTQFACAVSTLNHTSAFAKAYEKGVNKADYWEPTFDDSISLLAKLPTIAAKIYQNSYKGGGSLPAQVDPEQDWSYNFAAMLGKGGKENENFQDLLRLYLALHGDHEGGNVSAHATHLVGSALSDPFLSYSAGLQGLAGPLHGLAAQEVLRWILKMQSHIGTSFADADVKDYLWSTLKSGQVIPGYGHAVLRKPDPRFEALMKFASSRPEIARDPVFQLVKRNSEIAPGVLKEHGKTKNPFPNVDSSSGVLFHHYGFHETLYYTATFGVSRGLGPLAQLIWDRALGLPIERPKSINLEGLLKLAEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA64</accession>
    <name>A0A8H6CA64_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Allantoin permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003577</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149187.1">
        <property type="nucleotide sequence ID" value="XM_037294499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA64"/>
    <dbReference type="GeneID" id="59331988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF0">
        <property type="entry name" value="PURINE-URACIL PERMEASE NCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034463176" description="Allantoin permease" evidence="8">
        <location>
            <begin position="19"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="311"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="37912" checksum="D75BC1753DC4A0B5" modified="2022-01-19" version="1">MPIFGICLFTWAVTASHGFGPLLRIPNKIEDGMSLGYAFCYAITTAISASSTFAINIPDLTRYARNPRTATIAQAICLPVCMTLIYFLGVVMAASSQVIYGQIQWNPLTIVLMWDNRAAKFFVGLLFAFAMIGTNVAGNSVPFANDTMALFPKYMNLRRGQFLCAILGFAICPWKIEASAQSFLAFLNGYSAPFLGPVAGVLLSDFFLVRRSGELNVYQLYKPSGLYWYTAGINVRAVAAFTVGMVPQLPGLAYQINPMIKGVSRNYVDFTSLGWLEGLLFSGFAYHILCLSFPFPTRTDEEDKAAWAIVGHDGTRPSTSSTPPQDEVEKAVPLQDAISAPESSSGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA83</accession>
    <name>A0A8H6CA83_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Nucleoside transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_003927</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148896.1">
        <property type="nucleotide sequence ID" value="XM_037294849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA83"/>
    <dbReference type="GeneID" id="59332338"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015851">
        <property type="term" value="P:nucleobase transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000002">
        <property type="entry name" value="Purine-cytosine permease fcyB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF8">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G03000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="415"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6219461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="59465" checksum="F06BBFB3C2E374CD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAG6</accession>
    <name>A0A8H6CAG6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149378.1">
        <property type="nucleotide sequence ID" value="XM_037294690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAG6"/>
    <dbReference type="GeneID" id="59332179"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="128" mass="14066" checksum="B52E2520893CE9AF" modified="2022-01-19" version="1">MSSSTRSEDRVIDPHSASLEEEHKASLSKLTKRKRNVEDTEGGGAHEAGLLGVKRHCAEDHKMDQSAQSDQQGASNQQMSDTKGQGVDEMNIDSNNKEGELDPKLMEATKKMGIKPGNEVKNDMDWAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAH5</accession>
    <name>A0A8H6CAH5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149455.1">
        <property type="nucleotide sequence ID" value="XM_037294767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAH5"/>
    <dbReference type="GeneID" id="59332256"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF64">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="462" mass="50577" checksum="59EAEA06F8358E50" modified="2022-01-19" version="1">MQDFTANEAHLSTLDQISPQAYTRFILCFGLDSGSRHDEATQSLRHGLEATAIQIPALKSLVISVTNAKGRETKDLRQHDVTIFTVKDLTTSRLDFGEIRSQGFPSQVFDAKTLCATGVFVVPGSPVPTFLAQANLITGGLLLGLSVWHGALDGTAITTILRLWAQNCRATKDPVNDSIDTFTLSAAAFDNSRLSKVSGLQRGSVEDHPEFLLLPEAPTALPPGLTQTLKTQIFHISPASISALKDLANPRHSLSPQKEYSWISTNTAISALLWRSVMFATYGHERPITDSVSMFSLPLNGRTRMDPPLSPDLLASVWCFQDSRLPVNALLEANLADIALIVRRNTDRVDASYIDSLINMIDSVPNPSLLTPLVFTDVLKTCLILTSWAGFAMYDFDWGNALGGTCERVRTVASGMFNGMQVVLPELTKEMGGGLEIVVGLEDDAMERLKRDEVWMKFARLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAU6</accession>
    <name>A0A8H6CAU6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chitinase</fullName>
            <ecNumber evidence="3">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_003855</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Dual chitinase/transglycosylase that plays a role in cell wall architecture. Chitinase and transglycosylase activities are coupled. Required for the polysaccharide cross-linking at the septa and the cell wall. More specifically, transfers chitin to 1,6-beta-glucan in the cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149465.1">
        <property type="nucleotide sequence ID" value="XM_037294777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAU6"/>
    <dbReference type="GeneID" id="59332266"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02183">
        <property type="entry name" value="GH16_fungal_CRH1_transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000152">
        <property type="entry name" value="Cell wall glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF27">
        <property type="entry name" value="GLYCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034369581" description="chitinase" evidence="15">
        <location>
            <begin position="22"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="16">
        <location>
            <begin position="46"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6220030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="374" mass="40553" checksum="D4AD90EE173DC532" modified="2022-01-19" version="1">MSLLLASCLTLLPYLLNPAVAQTWTSCNPLNGTDCPHDLALGTSNASFTLNSTSSFESSLWNTTAGALTYNSSGAAFTIAQKGDAPTIQSKFYIFFGEVEVWLKAAPGQGVVSSVVLQSDDLDEIDQEFIGNNNTHAENNYYGKGNTTDAYERAKWYPLPDGGSPTEGYHNYTTRWTNGSLEWYIDGSIVRTLAYADANGGASYPQTPMNVRIGVWAGGDNENSNYTIQWAGGEIDYSKGPYTMFVQSVRITDYSTGSEYKYGDTTGTSQSIQSVPGNSTALKILTAPPPESVTQRFNGLSHGAKIGIFSTVVAAIAVCVGMWALCCVKQRRAGKAERRVADAMYEKDTAELMAYRARMGKGGYASEREMEDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAW0</accession>
    <name>A0A8H6CAW0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dynamin GTPase</fullName>
            <ecNumber evidence="2">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_003284</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149588.1">
        <property type="nucleotide sequence ID" value="XM_037294207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAW0"/>
    <dbReference type="GeneID" id="59331695"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    <feature type="domain" description="Dynamin-type G" evidence="14">
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            <begin position="735"/>
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            <begin position="584"/>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6220153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="822" mass="90578" checksum="07ABD14C58270CC6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAY7</accession>
    <name>A0A8H6CAY7_9LECA</name>
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        <recommendedName>
            <fullName evidence="5">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003661</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">RNA-binding nucleolar protein required for pre-rRNA processing. Involved in production of 18S rRNA and assembly of small ribosomal subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037294583.1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pumilio homolog 9"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="PUM-HD"/>
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    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
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    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF13">
        <property type="entry name" value="PUMILIO HOMOLOGY DOMAIN FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
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        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="PUM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="621"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="642"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="678"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="714"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="751"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="787"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="895"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="194"/>
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        </location>
    </feature>
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        <location>
            <begin position="386"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="489"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="956"/>
            <end position="998"/>
        </location>
    </feature>
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        <location>
            <begin position="1029"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="105"/>
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    </feature>
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        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="239"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="261"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="501"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="960"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="975"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1033"/>
            <end position="1060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1073" mass="118618" checksum="B50180FCA03199EE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAZ2</accession>
    <name>A0A8H6CAZ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome c oxidase assembly factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_003329</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Required for assembly of cytochrome c oxidase (complex IV).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Component of 250-400 kDa complexes called cytochrome oxidase assembly intermediates or COA complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the COA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149633.1">
        <property type="nucleotide sequence ID" value="XM_037294252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAZ2"/>
    <dbReference type="GeneID" id="59331740"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041752">
        <property type="entry name" value="Coa3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018628">
        <property type="entry name" value="Coa3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15642:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 3 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15642">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09813">
        <property type="entry name" value="Coa3_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c oxidase assembly factor 3 mitochondrial coiled-coil" evidence="11">
        <location>
            <begin position="20"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="61"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003064"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007035"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011351"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6220198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="97" mass="10715" checksum="B44DD25BBCB8634E" modified="2022-01-19" version="1">MAILPRSSYYDKHYRPAASLIRARQPYLIRNMVTGLALCAFTIGVYSWTINAVAQDDFSDVPIPDAPAQSPHSPHTGLEKSVSAAALDEQKEKRLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB34</accession>
    <name>A0A8H6CB34_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149508.1">
        <property type="nucleotide sequence ID" value="XM_037294127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB34"/>
    <dbReference type="GeneID" id="59331615"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36578:SF1">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36578">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034047303" description="Apple domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="35510" checksum="7B644F1ED343A4D7" modified="2022-01-19" version="1">MYIPFILAALAALTSALPSPQLIDLVAIEEYPDAVRVTAPIDIVKDVVPSVPAAPIRPLTSIISSSKRDLTVEKRDGDCSPYPTGSGPVPSPDTVDAFESFPTFAAMATAAPTPDGYEVVFINSNASLTASDYMGLYTLTTYDTLGCASQCDQASGCQAFNMYIERDPSLNASADNCPNPPSTTNYKCTLWGAPVSSSSLALTPTTKTPLPPACDGYNGPTELGGAINAPPSSGSYMGYQYFPFSQSQGYDPQTCADACNAQTTYDSENLAADGSYMACIFFNAYVLSEDGIPQGLYCSMYNETWDASYGTNYGQYQGDDRYTVSRSYSYALM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB67</accession>
    <name>A0A8H6CB67_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tripeptidyl-peptidase II</fullName>
            <ecNumber evidence="4">3.4.14.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_003424</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Secreted tripeptidyl-peptidase which degrades proteins at acidic pHs and is involved in virulence.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal tripeptide from a polypeptide.</text>
            <dbReference type="EC" id="3.4.14.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.10" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149727.1">
        <property type="nucleotide sequence ID" value="XM_037294346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB67"/>
    <dbReference type="GeneID" id="59331835"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000015">
        <property type="entry name" value="Tripeptidyl peptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF19">
        <property type="entry name" value="SERINE PROTEASE AORO, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10250)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 16" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0843">Virulence</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034263453" description="tripeptidyl-peptidase II" evidence="18">
        <location>
            <begin position="18"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="20">
        <location>
            <begin position="247"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="19">
        <location>
            <begin position="770"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="19">
        <location>
            <begin position="871"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="662"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="679"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="710"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="16">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="16">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="16">
        <location>
            <position position="573"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="614"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="615"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="633"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="635"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6220292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="958" mass="102891" checksum="ED02589D695E018B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB68</accession>
    <name>A0A8H6CB68_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Yeast cell wall synthesis Kre9/Knh1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149634.1">
        <property type="nucleotide sequence ID" value="XM_037294253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB68"/>
    <dbReference type="GeneID" id="59331741"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052982">
        <property type="entry name" value="SRP1/TIP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633:SF1">
        <property type="entry name" value="GPI ANCHORED SERINE-THREONINE RICH PROTEIN (AFU_ORTHOLOGUE AFUA_1G03630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05410)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034788996" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="4">
        <location>
            <begin position="34"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="229" mass="22172" checksum="77DF0C1A046CF90F" modified="2022-01-19" version="1">MRVAPLSLIACLLAAVAAQSGPTSNPPTTLPGFTVTAGTSSTFTWTPTTSGTVTLTLRNGPTSDLNKGIVLASGIANSGSYTFTVPAGTASGAYTIEISSDSNASDANYSDDITVNGSKSVGSASPSPSTLTTTASSSGSYSSASSSIDTSTTTLNSETSATSHASTSKSASSSSTSGATRSASSASGTSAPTISTSTPTGSSKGANAGTVLRVNNGLLAVVLGILAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBC1</accession>
    <name>A0A8H6CBC1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_003432</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149735.1">
        <property type="nucleotide sequence ID" value="XM_037294354.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331843"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056501">
        <property type="entry name" value="NAD-bd_HRPKS_sdrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF50">
        <property type="entry name" value="HIGHLY REDUCING POLYKETIDE SYNTHASE SRDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23114">
        <property type="entry name" value="NAD-bd_HRPKS_sdrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="10">
        <location>
            <begin position="276"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="11">
        <location>
            <begin position="1065"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="8">
        <location>
            <begin position="1065"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="8">
        <location>
            <begin position="1211"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="8">
        <location>
            <position position="1097"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="8">
        <location>
            <position position="1279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6220300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2196" mass="240225" checksum="E40A4923E9C87BDF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBE4</accession>
    <name>A0A8H6CBE4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotide-diphospho-sugar transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149890.1">
        <property type="nucleotide sequence ID" value="XM_037293812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBE4"/>
    <dbReference type="GeneID" id="59331299"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="38670" checksum="72B4B8538AB52D80" modified="2022-01-19" version="1">MNKRFQATVGTLIVIVILAFTFDPLNLARNHPSSSKSSTSSLIRNSPYAFVTLLAPNPKLDNKTVPDDEDEYFVGTRVLAYQLLHAPRTGTNTSIPFVVLATPDIPESKLERLRKDGATVKVIERISESWMKPGLQRWRDMMSKLRMLELTHFEKVLFMDADMYIAERMDGIFTDPTTEPLTPKPELAVKDEGPLPRSYIFSAQQYMEGRVHPYPPPAGDYFSGGFFLCQPSIEMFNYYLTIAKIKDRFNSNAMEQGLLNYAHRRAGPMPWTDIYYKWTTSWPSMKEYEAGAHSLHEKWWDEKIRLDPDLRKLWYIARGEMDGYYRGSEQALELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBI8</accession>
    <name>A0A8H6CBI8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003378</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149681.1">
        <property type="nucleotide sequence ID" value="XM_037294300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBI8"/>
    <dbReference type="GeneID" id="59331789"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039727">
        <property type="entry name" value="SE/Ars2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007042">
        <property type="entry name" value="SERRATE/Ars2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021933">
        <property type="entry name" value="SERRATE/Ars2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165">
        <property type="entry name" value="ARSENITE-RESISTANCE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165:SF0">
        <property type="entry name" value="SERRATE RNA EFFECTOR MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04959">
        <property type="entry name" value="ARS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13821">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12066">
        <property type="entry name" value="SERRATE_Ars2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="692"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="154"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="363"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="499"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="592"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="812"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="508"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="865"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="902"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="911" mass="102778" checksum="8710A218DD0472B8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC83</accession>
    <name>A0A8H6CC83_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002464</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150219.1">
        <property type="nucleotide sequence ID" value="XM_037293390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC83"/>
    <dbReference type="GeneID" id="59330877"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="25470" checksum="88DC715BDFA37812" modified="2022-01-19" version="1">MKRSLFQLPSLGSVCLPCRQRIARAHAFPPFRIPQKRLLTTTSRDLADTNPASSNISATGSVADLSQSFTSRFSDLSNEPIIPEGLMRKANPSLTDMPFIRPHHLHIYATRHNCHITLTAGNRDPIISVSSGNIGFKKAQRGSYDAAYQLGAYVMGMIKNQAMLDENYPGKGGQIRQLEVVLRNFGPGREAVSKILLGSEGRALRTRIVRVMDGTRLKFGGTRGKKPRRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCJ1</accession>
    <name>A0A8H6CCJ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Nitrogen permease regulator 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 5">Required for meiotic nuclear division protein 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002488</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 5">Mediates inactivation of the TORC1 complex in response to amino acid starvation. Required for meiotic nuclear division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Vacuole membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the NPR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150243.1">
        <property type="nucleotide sequence ID" value="XM_037293414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCJ1"/>
    <dbReference type="GeneID" id="59330901"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056603">
        <property type="entry name" value="HTH_NPRL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005365">
        <property type="entry name" value="Npr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153">
        <property type="entry name" value="CGTHBA PROTEIN -14 GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153:SF5">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24064">
        <property type="entry name" value="HTH_NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03666">
        <property type="entry name" value="NPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0469">Meiosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GATOR1 complex protein NPRL3 C-terminal HTH" evidence="7">
        <location>
            <begin position="758"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="82"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="233"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="670"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="111"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="705"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="813" mass="89796" checksum="E9B0194232991FCA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD14</accession>
    <name>A0A8H6CD14_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mago nashi protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002722</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mago nashi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150476.1">
        <property type="nucleotide sequence ID" value="XM_037293647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD14"/>
    <dbReference type="GeneID" id="59331134"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11295">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1560.10:FF:000001">
        <property type="entry name" value="Protein mago nashi homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1560.10">
        <property type="entry name" value="Mago nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004023">
        <property type="entry name" value="Mago_nashi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036605">
        <property type="entry name" value="Mago_nashi_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638:SF0">
        <property type="entry name" value="MAGO HOMOLOG, EXON JUNCTION COMPLEX SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638">
        <property type="entry name" value="PROTEIN MAGO NASHI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02792">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89817">
        <property type="entry name" value="Mago nashi protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17664" checksum="6636910AEED65CA6" modified="2022-01-19" version="1">MAASSEPFYVRYYSGHSGRFGHEFLEFDFRTLGDGRSASARYANNSNYRNDSLIRKEMCVSSLLIAEIKRIVKESEIMKEDDTKWPEKNKDGRQELEIKLGSEHISFETAKIGSLVDVTESADPEGLRVFYYLVQDLKALVFSLISLHFKIKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDG3</accession>
    <name>A0A8H6CDG3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_002318</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150897.1">
        <property type="nucleotide sequence ID" value="XM_037293244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDG3"/>
    <dbReference type="GeneID" id="59330731"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000009">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="17">
        <location>
            <begin position="675"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6221462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1052" mass="119041" checksum="2AA2D4A35170C6D4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEF2</accession>
    <name>A0A8H6CEF2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151510.1">
        <property type="nucleotide sequence ID" value="XM_037292091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEF2"/>
    <dbReference type="GeneID" id="59329577"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF13">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="2">
        <location>
            <begin position="251"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="677"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="706" mass="79585" checksum="087FA2AF30B41DAF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF29</accession>
    <name>A0A8H6CF29_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Micro-fibrillar-associated protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151638.1">
        <property type="nucleotide sequence ID" value="XM_037292219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF29"/>
    <dbReference type="GeneID" id="59329705"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033194">
        <property type="entry name" value="MFAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15327">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Micro-fibrillar-associated protein 1 C-terminal" evidence="2">
        <location>
            <begin position="125"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="498" mass="56981" checksum="DDFDDA2B9C90095B" modified="2022-01-19" version="1">MSSKKMTANPVRPARYRPGKAVAEEPSSDEEDASAGEEEQSTPAPRAPAPTASSFPSDATRIAGSLKNVNLNKRRRQAAAQEAARVEAEKAARVAEEEGFVTEESEEQESSDEELGSGSGSDENSSEEEAPKVLLRPTFIKKDKRKDSVVVPDTKTEEDTWAEEQARRKEKADVMIQEQLEKDAAARAAGKKDWDDDEIEEVQQVDDTDDLDPEIERAAWKLRELKRVKREREAIEIAEKEREEIDRRRNLSKEERDAEDKDFIEKQREEQEGKGKMGFMQKYYHKGAFFQDDAKAEGLDRRDIMGSRYQDDSNRELLPQYMQIRDMTKLGRKGRTKYKDLKSEDTGRWGTSEGRGPRKADGTFDERFMPDQGTGGKGGSGANTIQVGERRRPPEGAPEGPRGRDRGGRFDEDGGRRSPPRRSYSRSQSPPRRRDHSYSRSPLPRRDRDRSRGRSYSYSRSPPPRRDRYRDEPSRRKRSPSPYQDRYGSDKRRRVDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF63</accession>
    <name>A0A8H6CF63_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dynamin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_001448</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151797.1">
        <property type="nucleotide sequence ID" value="XM_037292378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF63"/>
    <dbReference type="GeneID" id="59329864"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051646">
        <property type="term" value="P:mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Transmembrane GTPase Fzo1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF0">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="13">
        <location>
            <begin position="279"/>
            <end position="583"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="138"/>
            <end position="222"/>
        </location>
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        <location>
            <begin position="500"/>
            <end position="556"/>
        </location>
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            <begin position="40"/>
            <end position="51"/>
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        <location>
            <begin position="194"/>
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        </location>
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            <begin position="211"/>
            <end position="220"/>
        </location>
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        <location>
            <begin position="509"/>
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    </feature>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6222362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="947" mass="103961" checksum="32CFA077E3649472" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFB4</accession>
    <name>A0A8H6CFB4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7702 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001523</name>
    </gene>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151872.1">
        <property type="nucleotide sequence ID" value="XM_037292453.1"/>
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    <dbReference type="GeneID" id="59329939"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056119">
        <property type="entry name" value="DUF7702"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42109:SF2">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42109">
        <property type="entry name" value="UNPLACED GENOMIC SCAFFOLD UM_SCAF_CONTIG_1.265, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF7702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7702" evidence="2">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="264" mass="28070" checksum="EFC4EAC1A4F5588E" modified="2022-01-19" version="1">MLSERENIATAELAVYIPSIALAIAIIFKHGFSKGRPRIYLILFCGLRTASAILEILSAKNPANRDDATWTAILGSIGLSPLFLVASGLLRRVNDFIAGGTSRTRLLEMLHIPIMLALILSIVGGTRISSSDTSKLNSGETFGKAGAIISLISSVVIVAFAMLAMAESRDLPRGEKCILYAVLVSLSFLAVRLLYSLLADFEDNNTFNIVGGNATVQLCMAIIQEFVVTFFFLVADLVAPALDSLVNGAGGIPMNSYNVGKGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFN4</accession>
    <name>A0A8H6CFN4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001432</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037292362.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59329848"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF31">
        <property type="entry name" value="DCN1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DCUN1" evidence="4">
        <location>
            <begin position="1228"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="418"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="645"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="858"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="932"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="418"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="502"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="571"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="860"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1000"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1052"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1095"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1126"/>
            <end position="1141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1446" mass="160389" checksum="B0D1F726A074D242" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFS9</accession>
    <name>A0A8H6CFS9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152020.1">
        <property type="nucleotide sequence ID" value="XM_037291658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFS9"/>
    <dbReference type="GeneID" id="59329139"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="231"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="741" mass="82208" checksum="ABF0321CA7DEAE31" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFU0</accession>
    <name>A0A8H6CFU0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151926.1">
        <property type="nucleotide sequence ID" value="XM_037291564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFU0"/>
    <dbReference type="GeneID" id="59329044"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039931">
        <property type="entry name" value="EEIG1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456:SF1">
        <property type="entry name" value="C2 NT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="359" mass="39597" checksum="FC07DED4E083A103" modified="2022-01-19" version="1">MSFSTLFRMMQQLTVPSTVPKNRRPKFDLQLKIIDLNNVPLVSGTSYVKWHLPSSTSAEHRGHTSKAGIRDHKVLWEYVKTLPVRLTVDRNGMLQESEIHLEVLQEYSPGTRAERIVLGNVKLNLAEFVGGSDDGEEAVTRRYLMQESKINSTLKIGVYMKQTEGDKTFIAPPLKTAPVFGGIAGIMTAEQADGDDGSNVPSMSSHARESGRLQDMYRRTLAASWLAQDGELPADQCIEDIFAGGDGWATDHDPGVNVSPRLGGSTEEDDSDGDSRTLRGHKRHTSGTGSRGTIRPTRQERKSSKGDQGHTGAVSGRGSIADHIHSGSDAKNHAHRRMHEVDEFTSREDLRSWQISNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG68</accession>
    <name>A0A8H6CG68_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000918</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152213.1">
        <property type="nucleotide sequence ID" value="XM_037291851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG68"/>
    <dbReference type="GeneID" id="59329336"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF32">
        <property type="entry name" value="FAD-LINKED OXIDOREDUCTASE AFOF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034369583" description="FAD-binding PCMH-type domain-containing protein" evidence="6">
        <location>
            <begin position="17"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="7">
        <location>
            <begin position="56"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="151" mass="16260" checksum="96A2BE85697C835A" modified="2022-01-19" version="1">MLFHVGLLALPIIVAATNFQQTNLTALFSPSLSPGAEIFLPTNPDDDEDLTQRWSLAFEPSFVLNIKPATNEDVQNIVKIASESHIPFFATGGAHGAELGFATVKNAVNIDLGNFNSTELDAANNLLTVGGSTTFSQFYDLLYNAGKEIRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGA0</accession>
    <name>A0A8H6CGA0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000708</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152007.1">
        <property type="nucleotide sequence ID" value="XM_037291645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGA0"/>
    <dbReference type="GeneID" id="59329126"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008477">
        <property type="term" value="F:purine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02651">
        <property type="entry name" value="nuc_hydro_IU_UC_XIUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304:SF4">
        <property type="entry name" value="URIDINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="5">
        <location>
            <begin position="43"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="390" mass="42137" checksum="102F38E1B727CD7C" modified="2022-01-19" version="1">MEDGRAKQIPLWLDCDTGTARQGTTYGQPQPQPSPSNAKPSPQDAFAILLAAYDPCLHLLGISTVHGNASLAQTTNNTLSVLAAIGRPDVPVHPGAAKPFCRNAVHAADIHGASGLDGTTLLPKPAAEPVTKPNAVLAMRHALMEQPEGTAWLVATGTLTNVALLFATFPELVEHLKGLSVMGGAIGGGFTDAPMGHVKGEGERFGNWTRWAEFNIYCDPEAAESIFSNRKLAAKTTLIPLDLTHQVLATREVQQTLLTGRPLTTPSASAMNLRQMFHDLLLFFAHTYSEVYGITEGPPLHDPIAVAVLLEPLMGPIMRFADNGGERWHVRIVTDGLHSRHDEERGQVGRTVVTKSDHGGVRIPRSMQVESFWRQIGRCLEEAERAILDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGA2</accession>
    <name>A0A8H6CGA2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000978</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152273.1">
        <property type="nucleotide sequence ID" value="XM_037291911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGA2"/>
    <dbReference type="GeneID" id="59329396"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12400">
        <property type="entry name" value="RRM_Nop6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000376">
        <property type="entry name" value="Putative RNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034228">
        <property type="entry name" value="Nop6_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF51">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="92"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="243" mass="27215" checksum="2B456B45E408C379" modified="2022-01-19" version="1">MGSDLKRKEARKRKFEGQHSESLLDAGVKSGLEGTLAGGPLHKKSNQAPPPPSSSERIPTAEKIAARKVAVVDESTDQGKEEESAIQKAQRFIVFIGNLPYTATDESVSKHFAKINPKSIRHRKEKDTEKSKGFAFLEFEGYDRMKTCLKLYHQSDFEDGESPARKLNVELTAGGGGGKSKDRKSKLRAKNERLNEQRKRRNQEEEKRRKIPQKDSQPTHALGNNGDIHPSRRSRVATDSLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGI3</accession>
    <name>A0A8H6CGI3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000829</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152127.1">
        <property type="nucleotide sequence ID" value="XM_037291765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGI3"/>
    <dbReference type="GeneID" id="59329247"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051414">
        <property type="entry name" value="Adenylate-forming_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439:SF2">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439">
        <property type="entry name" value="PHENYLACETATE-COENZYME A LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="3">
        <location>
            <begin position="343"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1082" mass="118179" checksum="F53EE2E416D1C6C9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHM0</accession>
    <name>A0A8H6CHM0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152800.1">
        <property type="nucleotide sequence ID" value="XM_037291365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHM0"/>
    <dbReference type="GeneID" id="59328845"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="211" mass="24150" checksum="243D05666903BA51" modified="2022-01-19" version="1">MVSVPSAVVSLIRKTEPVNIRGGDFYIDFEFVSLGGFREDDLLIQRFPVEIAVRRLLGQVVIDTPIKYDKSIPELLAGIPRTGSTANFSWAVMRKVYGQEKETFGKTWDELRHLLHDADMEKDSYVPDARWAMRALIDLAHRDDTRAEEFGKGELRIDEQISRLQEQLVMEYDKDDESDDGQSVKDVDSLETQIAHVEAMKAMTGEEKRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHS8</accession>
    <name>A0A8H6CHS8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell cycle control protein cwf14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000324</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BUD31 (G10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152698.1">
        <property type="nucleotide sequence ID" value="XM_037291263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHS8"/>
    <dbReference type="GeneID" id="59328743"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001748">
        <property type="entry name" value="BUD31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018230">
        <property type="entry name" value="BUD31/G10-rel_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411:SF0">
        <property type="entry name" value="PROTEIN BUD31 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411">
        <property type="entry name" value="PROTEIN BUD31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01125">
        <property type="entry name" value="BUD31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00322">
        <property type="entry name" value="G10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00997">
        <property type="entry name" value="G10_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="16449" checksum="96F2DF83BBC56C49" modified="2022-01-19" version="1">MPPVRSARNRKPPPDGFDDIEDTLLEFANKMKDAENASHEGKKRHEVLWPIFQISHQRSRYIYDLYYDKEAISKLLYEWLIKNGYADGILIAKWKKQGYEKLCCLRCIQTKETNFNATCICRVPQAQLKEDQTIECAEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI24</accession>
    <name>A0A8H6CI24_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000578</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152952.1">
        <property type="nucleotide sequence ID" value="XM_037291517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI24"/>
    <dbReference type="GeneID" id="59328997"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015244">
        <property type="term" value="F:fluconazole transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF23">
        <property type="entry name" value="FLUCONAZOLE RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="500"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="567"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="160"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="601" mass="66011" checksum="C5CAA86187342D3E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI74</accession>
    <name>A0A8H6CI74_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010629</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153115.1">
        <property type="nucleotide sequence ID" value="XM_037301481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI74"/>
    <dbReference type="GeneID" id="59339019"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032161">
        <property type="term" value="C:cleavage apparatus septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043934">
        <property type="term" value="P:sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000260">
        <property type="entry name" value="Cell division control 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="7">
        <location>
            <begin position="34"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="310"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="340" mass="38591" checksum="D3B23D58AED5674F" modified="2022-01-19" version="1">MATAMSPQQGPVMPKSHVGFDSITTQIERKLLKRGFQFNVICVGQTGLGKSTLINTIFASHLIDSKGRLSPSEPVRSTTEIQSVSHVIEENGVRLRLNIVDTPGYGDQVNNDRCWDPIVKYIKDQHSAYLRKELTAQRERYIQDTRVHCCLFFIQPSGHALKPIDIVVLKKLSDVVNVVPVIAKSDSLTLEERMAFKERIKEEFTFHNLRMYPYDNDEQDEEERATNSSIKDLIPFAVVGSENSIVVGGRSVRGRQNRWGVINVEDENHCEFVSLRNFLTRTHLQDLIETTSQIHYETFRAKQLLALKENSAAGQGSRPISPSADRELSRQSQRMTMNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJR4</accession>
    <name>A0A8H6CJR4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009986</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153659.1">
        <property type="nucleotide sequence ID" value="XM_037300842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJR4"/>
    <dbReference type="GeneID" id="59338378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050023">
        <property type="term" value="F:L-fuconate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03324">
        <property type="entry name" value="rTSbeta_L-fuconate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000007">
        <property type="entry name" value="Mitochondrial enolase superfamily member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034610">
        <property type="entry name" value="L-fuconate_dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018110">
        <property type="entry name" value="Mandel_Rmase/mucon_lact_enz_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046945">
        <property type="entry name" value="RHMD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794">
        <property type="entry name" value="ENOLASE SUPERFAMILY, MANDELATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794:SF58">
        <property type="entry name" value="MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00111">
        <property type="entry name" value="L-fuconate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00270">
        <property type="entry name" value="L-galactonate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00909">
        <property type="entry name" value="MR_MLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="5">
        <location>
            <begin position="198"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="447" mass="49982" checksum="22E0FB0915737280" modified="2022-01-19" version="1">MVTITCFASKDVRFPTSLDKTGSDAMNPAGDYSSAYCILQTDSPHTGHGMTFTIGRGNDIVCLAIENLATRLKGRTLESLTANWGQTWRYLVSDSQLRWIGPEKGVIHLALGAVVNALWDLWAKVLQKPVWRIVAEMSPQEFVRCVDFRYITDAITPEEAIEMLCKEESGKAQRLQEAENNHAVPAYTTSAGWLGYGEDKMERLLQETLEEGYKHFKFKVGTSISSDIRRLTIARNVIGYDRGNILMIDANQVWSVPEAIAYMKELAPFKPWFIEEPTSPDDVLGHKAIREALKEYGIGVATGEMCQNRVVFKQLLMGGAIDICQIDACRMGGVNEVLAVMLMAKKFDIPIVPHSGGVGLPEYTQHLSTIDYVCVSGKLSILEYVDHLHEHFNHPAVIKNGYYQTPTEPGYSVEMKEESMEKYGFPGEEGGFWRSEEARPILEGVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJT1</accession>
    <name>A0A8H6CJT1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153716.1">
        <property type="nucleotide sequence ID" value="XM_037300899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJT1"/>
    <dbReference type="GeneID" id="59338435"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07600">
        <property type="entry name" value="BAR_Gvp36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018859">
        <property type="entry name" value="BAR_dom-cont"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10455">
        <property type="entry name" value="BAR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="34063" checksum="453744457C7B89DD" modified="2022-01-19" version="1">MDKFQAFGKNITSSFSPFAARTQQYVKEQLGTADDKTELPPDYIELEKRVDALKQVHQKLLQVTSQYSNEAYDYPPNIRESFNDLGRTITDKVNLLSHAKSPAEAQAAFTAPPSAKPQPKTFPHAIARASLAGSQLLIQTNPSSSSGEDPLATALERYALASEKVGEARLAQDQQIQQRFLAGWSTTLNTNLMFATRARRGVENARLGLDATKAKGKGGKGEEDLPMSDEHRAEVERKEDEFVGVTEEAVGVMKNVLDTPEPLRNLADLIAAQLEYHKKAYEILSELAPVVDGLQVEQEASYRKSRDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJT5</accession>
    <name>A0A8H6CJT5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_011008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153491.1">
        <property type="nucleotide sequence ID" value="XM_037301857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJT5"/>
    <dbReference type="GeneID" id="59339398"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="273" mass="29493" checksum="6F939D3071BC5BE7" modified="2022-01-19" version="1">MSMDPTPNPIPIPATAPITINPSATSRKRTHEEISPEDESMGGALPSSRTPGISPTAIYGEGMARINPLTGTATTAETQTGTWFEDQLEMKLQPGVEETAAQITVSDGELPKRKVQRRDSSSNADVAMAANPTESPKVTVGGPNTYRSSHELGIGWTYVGVYSEFVAMARGFSRYIDNHYPLTDSELLFESKSLEAYLVSSNQGYFLFAEDLTEGRLVARTWEDTLANLQSSPVRFSWAQPLFAARTPDTGRESDNGTGSSAMAGEVGDIDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJU8</accession>
    <name>A0A8H6CJU8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Meiotically up-regulated gene 154 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010073</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153746.1">
        <property type="nucleotide sequence ID" value="XM_037300929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJU8"/>
    <dbReference type="GeneID" id="59338465"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018819">
        <property type="entry name" value="Nur1/Mug154"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293:SF1">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10332">
        <property type="entry name" value="DUF2418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="328"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="349"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="399"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="49250" checksum="741998D19D0B5146" modified="2022-01-19" version="1">MAPRLIRRRPLVERIKAYLNPLDFLLWLSEELDSNDWDQWKKEWANPIGFLLNVVFLIARANTAYSARNIGDDVFGDEVAYTPWLAWFAAFLVHLLSLLSFVNAAYTFNRRRHYRLFESSVDAPPSTPSAHRVHVDSSPMASSPLRFLSNILAGENAEARSHPDATRDVWEVAVWDPTPISLRMFCLFSPGHILVYWLFLPTAVSDPRPSTTVITTMVLAGLLSAQMLLLQSSFSQQSKDASVIHKEVMNEYDTKYVHPRTQPQMRNVGTQYCNSGTSTDGMPQYADEDDSVDVYAPTFIINRGFHTRPNPNYAKHVDLDGSAWRAMPSGGISTSTAPSAQTPAHLRDSSSPIRPQTAMRQPQFRAAGTGDGGSLGVFSHANSPLRKSASTSFVGPQGQRERSLSPAKREGSPLKRSSLAPLPNGQRWGHLQGAGPRGESRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK02</accession>
    <name>A0A8H6CK02_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153637.1">
        <property type="nucleotide sequence ID" value="XM_037300820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK02"/>
    <dbReference type="GeneID" id="59338356"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="332" mass="37409" checksum="9CC29B09D5A16510" modified="2022-01-19" version="1">MRPPNSPLDHRQQDPKPPSPPDRFEPPSNKQSSVTGQHDLQTPGHEVKIQTASFGQKPPFNIRPRIQSDGAPSSIVSQHRISQFAIHDGEENSDEGEDDEDTSGEDDEEEGEDAGGASEAASHDKSEEDVKAAGRAFLALVHDAIKNTKNGVEPKRIAEQSLVLETRDPRPHHIQIDKTDMSTHPDLVLQLVFGKIKQRKKIGVVLEFKKRDHKLNDEEILGQEVSELLGAVLEKKAARKQKSYKSYLISWHGLKGYISRFYATKEYLNQLQENSRGLQDMDPPQVRRSEEFDLTKSEERVNFAIGCARIAVEELLQFERNQTSGLLERIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLH3</accession>
    <name>A0A8H6CLH3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154383.1">
        <property type="nucleotide sequence ID" value="XM_037300535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLH3"/>
    <dbReference type="GeneID" id="59338069"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="89" mass="10188" checksum="C8E581CEE63D0896" modified="2022-01-19" version="1">MSFLRNPQLLLGVANISAIVGSTYLLRSHHIYSLEEHEARMDELEGTLRGHIGLIEEALDRLEGKGTEADRNKKMNEYYSKRGRDEKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLW3</accession>
    <name>A0A8H6CLW3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008929</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154618.1">
        <property type="nucleotide sequence ID" value="XM_037299792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLW3"/>
    <dbReference type="GeneID" id="59337324"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF14">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="541"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="574"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="606"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="668"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="701"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="734"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="768"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="801"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="856"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="304"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="872"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="899" mass="100800" checksum="FE9AC3CD613C7A81" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMB2</accession>
    <name>A0A8H6CMB2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009881</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154588.1">
        <property type="nucleotide sequence ID" value="XM_037300740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMB2"/>
    <dbReference type="GeneID" id="59338276"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="18"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="346" mass="36162" checksum="DDA860257937B291" modified="2022-01-19" version="1">MTSATAETTAQLQCMQQGGPFKIVHVPKPTLAPEEVLIRQRVIALNLIDAKQRDLGILIPRWPRVLGIEGAGIIEAVGSDVRDLQPGDEVVGWEGSGAHEVSWGGAFQERVAVPAPFLAKKPKNIGLEEAASLPVCFVTAVCAIVNSLKIPLPFLQGMSTEGQAPSSILVLGGSSATGAAAIQLLRKAYPSLPILATSSVKHYARLNDLGATSVVDYKSPSVVADIKAASPGAAGVEVIIDCVSAGASQTDISDVLDPAGSKRYAAIITGVPVPVPEGVNKLDVSAWSMVDMQGGKQLIPSLTKLVEEGKYRVPLPVRVVGHGLEELPNVLDEVKTVSGEKVVLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN17</accession>
    <name>A0A8H6CN17_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histone acetyltransferases subunit 3-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009383</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NGG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155070.1">
        <property type="nucleotide sequence ID" value="XM_037300244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN17"/>
    <dbReference type="GeneID" id="59337778"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019340">
        <property type="entry name" value="Histone_AcTrfase_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10198">
        <property type="entry name" value="Ada3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="106"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="380"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="435"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="597"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="106"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="384"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="453"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="694" mass="76262" checksum="394D40D6F1826FFB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN54</accession>
    <name>A0A8H6CN54_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009333</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155020.1">
        <property type="nucleotide sequence ID" value="XM_037300194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN54"/>
    <dbReference type="GeneID" id="59337728"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="3">
        <location>
            <begin position="111"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="111"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="199"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="344" mass="37477" checksum="41021C1D8CE77DC8" modified="2022-01-19" version="1">MARPKKAEEKPKDSGSLQISVEDFVRTRNSVVTGLATLQSAVSDLSRAYIAHTDTVIGRGNASSLEQLNISNPLSGENGLFGGRGPTPAPTTEAPPEGKKRKRAPHDKNAPKRALTPFFLYLQTARPALATEMGSGHTAKEVQDEGGRRWREMTDKDKSEWQYRYAVNFARYRAQTDAYKAGKPIPEISDAEAKKLYETTKKDGKILAPADLDPGHIDDEDESDSSTTSDEDSPEPVKAPSPKRKKTAKNSVEKKPPSAKQTPAKAPEPAVAEPTSQSPEKTSPEKKKKASKKKETKGTGDMTDSKKEVVAEPASSPSKLTGQDSQQKQQKKKGRKRKSEPAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN65</accession>
    <name>A0A8H6CN65_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative phospholipase</fullName>
            <ecNumber evidence="4">3.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_009389</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36707"/>
            <dbReference type="EC" id="3.1.1.47"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the serine esterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.47" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155076.1">
        <property type="nucleotide sequence ID" value="XM_037300250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN65"/>
    <dbReference type="GeneID" id="59337784"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003847">
        <property type="term" value="F:1-alkyl-2-acetylglycerophosphocholine esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016715">
        <property type="entry name" value="PAF_acetylhydro_eukaryote"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272:SF7">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03403">
        <property type="entry name" value="PAF-AH_p_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018169">
        <property type="entry name" value="PAF_acetylhydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR018169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR018169-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6226523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="513" mass="56124" checksum="591324EB9AD78371" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPU5</accession>
    <name>A0A8H6CPU5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008717</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155582.1">
        <property type="nucleotide sequence ID" value="XM_037299581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPU5"/>
    <dbReference type="GeneID" id="59337112"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052606">
        <property type="entry name" value="DnaJ_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034209994" description="J domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="43"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="246"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="362"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="367"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="393"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6227274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="405" mass="45049" checksum="2E724B60A7904910" modified="2022-01-19" version="1">MKLSLFVLLCSGLLALVAGFTKDDHEIFRLRDEIQASEGPEVTFYDFLGVKASASQDEIVKAYRKKSRIIHPDKAKQSLIASKAKATSKPLLGLKNKEPGVHVNKAPTESEIQTAVKKASERYARLGVITEILKGPGRERYNFFLSSGFPKWRGTGYYYARFRPGLGSVLVGLFAFGGGLVHYAAMYVSWQRQRDYAERVIRDARRSAWGDDSGVRGIPGIDGAMTGMTASPPASLAQENGAAVLNRRQKRMQERENKKEKDKKTGKGLKRSGTSTPTPIESEPEQGPQGAKKKVQARNGKVLIVDSVGNVYVEEEDDNGERVELLLDPEEIPKPTFRRTVLFRLPVWTYHKIQSRIAGVQSNYEQSELEDLETANDGEDGDPEIKEDSRNGAARKRVKRNGKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQL9</accession>
    <name>A0A8H6CQL9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007788</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. FUS2 hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155994.1">
        <property type="nucleotide sequence ID" value="XM_037298658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQL9"/>
    <dbReference type="GeneID" id="59336185"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050261">
        <property type="entry name" value="FrsA_esterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22946">
        <property type="entry name" value="DIENELACTONE HYDROLASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22946:SF9">
        <property type="entry name" value="POLYKETIDE TRANSFERASE AF380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="127"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="298" mass="32221" checksum="409CBDAD2E4A6B44" modified="2022-01-19" version="1">MATPKTSLSTSRPPSTPPASLSAYENKGLSHPPNLDPLTNAQKQTCRVKPVTIKVNDDLYGAVPAFLHIPQNYRREQTEGREKTAAILLSGAGGGVVGPSSIYLSMADKLASLNKGIPVMRLDYRYPARNKYCVADVQAAMKYLETGYGLSKFVLVGWSFGGAPVYTLGGSEERVIGCATVASQTAETEGIGRLAPKPVLLMHGTGDRTLRYSRSQRLYDEYGTKGERELKLFDGDDHALTRNAREAETRLCEFVSKCAGVEIGGAEKEMMGQELIGDGDRVRLMKEGGALNGGERVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR77</accession>
    <name>A0A8H6CR77_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156109.1">
        <property type="nucleotide sequence ID" value="XM_037298773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR77"/>
    <dbReference type="GeneID" id="59336302"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015057">
        <property type="term" value="F:thrombin-activated receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003944">
        <property type="entry name" value="Prot_act_rcpt_4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01430">
        <property type="entry name" value="PROTEASEAR4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="360"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="446" mass="47781" checksum="4E1F96A8192178EF" modified="2022-01-19" version="1">MAALIQTIAVLDNSGKAISTSKHLVNVFREAKAAYRERKAEVVAGRHYEIEDKKARKGLEAATVYDNRPAAGPRKHSRPHHGNRRSSTDHRRPEEASRSESNTIYPSSHNRFSQELRTPRSPTFPHQELTRRHSGLDDVVVPRAPRLPPRSMTSPSSPSSIIDMDLAYGPIPPPLPVANPADDSEFKGLVNKVKALLEEADCLQYSVSATIASLQKNPDAMAAVALTLAEISNIATKMAPGVLVTMKGSAPAVFALLASPQFLIAGGVAVGVTIVALGGYKIIKKIRANNAVEDPGMDEMLEIGGDVSAIDNWRRGIAEAEEQSLGTSVDGEFITPEAFTLSKSNLNDKSASETRRKEVGSGSKKSSSAKGASETSSKTGSKGSSRGSSRGSSRGSSKGSEQGGSKGKREKKAKKPSPLRLMSRQFDTLSHVPLRYLGDDQQLPVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR99</accession>
    <name>A0A8H6CR99_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">proteasome endopeptidase complex</fullName>
            <ecNumber evidence="3">3.4.25.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_007856</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156062.1">
        <property type="nucleotide sequence ID" value="XM_037298726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR99"/>
    <dbReference type="GeneID" id="59336253"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000005">
        <property type="entry name" value="Proteasome subunit beta type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024689">
        <property type="entry name" value="Proteasome_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12465">
        <property type="entry name" value="Pr_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0888">Threonine protease</keyword>
    <feature type="domain" description="Proteasome beta subunit C-terminal" evidence="12">
        <location>
            <begin position="221"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6228128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="276" mass="29851" checksum="714B7A1ED5B10B25" modified="2022-01-19" version="1">MPGFDFSNHNRNAALHAQGVQLPKATSTGTTIVGAIFDGGVVIAADTRATSGPIVADKNCEKLHYIAPQIWCAGAGTAADTEFTTAIISSNLELHALSTGRKPRVVTCMTMLKQHLFRHQGHIGAYLVVAGVDPTGVGLFTVHAHGSTDKLPYVTMGSGSLAAMAVFETQWKQKMTREEAIKLTSDAIQAGIFNDLGSGSNVDVCVITAEKTTLMRNYITPNQRELKQRDYKFKRGTTAVLNEKIITREEISKYVTVHELSSDDDVVVVEKMDIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRH7</accession>
    <name>A0A8H6CRH7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156274.1">
        <property type="nucleotide sequence ID" value="XM_037298938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRH7"/>
    <dbReference type="GeneID" id="59336467"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898:SF2">
        <property type="entry name" value="NUCLEOPROTEIN TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898">
        <property type="entry name" value="NUCLEOPROTEIN TPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="202"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="427"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="530" mass="57896" checksum="042FF58C9C4A76D5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS58</accession>
    <name>A0A8H6CS58_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">60S ribosomal protein L44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008219</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156423.1">
        <property type="nucleotide sequence ID" value="XM_037299087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS58"/>
    <dbReference type="GeneID" id="59336616"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="129"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6228489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="22867" checksum="7F8B607E2374BFF8" modified="2022-01-19" version="1">MVSSPLSLHSRQSLQEKSLLALREMGTGGMRARRARGGGRGGYIGIELEEGWLQEQDPKKEKNAKGKNGSSVGVVLALESSFKPLRYSSPPQTGNTDAETFAPQVPKTRRTYCKGKECRKHTQHKVTQYKAGKASSVAQGKRRYDRKQSGYGGQTKPVFHKKAKTTKKVVLRLECTNCKTKAQLSLKRCKHFELGGDKKTKGAALVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7C5</accession>
    <name>A0A8H6F7C5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006399</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147422.1">
        <property type="nucleotide sequence ID" value="XM_037297297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7C5"/>
    <dbReference type="GeneID" id="59334800"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF165">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="32"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6217987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="378" mass="41456" checksum="50901628726F17B2" modified="2022-01-19" version="1">MSSPTGSNASAGNVLVAVSWTFFVVSLVVVALRFYADIFIIHLVRIDSYLTFLTFLLVIVSQVFTQISVHYGMGTHIDALTPNAVVLALKYCWVAQPFQLLALAFGKIAAVTYLATIHGPRNANIKLAFLWIVGLVQLLSGIVILGFIYSQCSPMTKLWNVEIPGTCNGRARNVYMAYLDGSLSAFSNLCISLYPTLIFWNLQMKTTKKVVLCLLFGTGVIAAVCAVFKTTKVSTLGKTTDITYDMTGLMTWTAVEQYTVLLAGSIPPLRPAIKELFNKASSTTYYRSRNTNTLRLDDEATLTSSTVSPGKTSAYTVGTKGRSEAFNGKRDSTEKILSEMGEGGIMMTTRIEVENRIYAQRMNASRDQMVARNGREGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7H2</accession>
    <name>A0A8H6F7H2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyl transferase family 25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_006665</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146998.1">
        <property type="nucleotide sequence ID" value="XM_037297562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7H2"/>
    <dbReference type="GeneID" id="59335066"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06532">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050757">
        <property type="entry name" value="Collagen_mod_GT25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002654">
        <property type="entry name" value="Glyco_trans_25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730:SF53">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01755">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 25" evidence="5">
        <location>
            <begin position="99"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6217563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="416" mass="46885" checksum="358A5E26B6E0D5CA" modified="2022-01-19" version="1">MSEYIKSTLANLPQKVHSLQSLSSYRLSLLNIQFPTINFQGKGSLPTRNPRAFIIFAFLLFLLFWKDIIRDVCSHVATRRLPVPVVEVSSIRNNTLGFQNIFVLSLPERGDRRTPLLAAANATNLTLTVLDAIRDRQIPQSDWPQWWGTNTWVPKDGELGCLMSHVRTWRKMIHENISTALIMESDVDWDMRIKDTMVGVGDGVKAIADWPFPDPQHPRDFSKELSPYGDKWDLMWIGHCGINADGDGRIYAFNDSSAADEDHAWNFGERPAEGHRPPGTRIVFQPRKTVCTTSYAMSLAGARKFEKLFKEANSPIDLKLWDHCEHDPTTRCVGTWPQVISMTESKTNIKHSGGGLSFGHEVTEEKIVAGNAIQISARVNAHLGLADKGPTKWKSEWVTGEKKKEETADDFTDKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8X1</accession>
    <name>A0A8H6F8X1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005607</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148498.1">
        <property type="nucleotide sequence ID" value="XM_037296516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8X1"/>
    <dbReference type="GeneID" id="59334013"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF31">
        <property type="entry name" value="AMIDOHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_7G05840)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="4">
        <location>
            <begin position="44"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="427" mass="48296" checksum="504AF76636AB7EFA" modified="2022-01-19" version="1">MPETVDQCKKLALPGQGDAFAEQMLDIHGERLEIMTQYNVELFVLSLASPGPQGQHDKEKAESLARRANDYMAKEVGKNPKRFAGFCVLSMHDPCQAAQELRRSVIELGLKGAIINDFQSAGPDGETMLMYDQPEYDHFWAAVQSPHLQKELWDPRWWLSIASLQFGQDVQRHILGMCANGVFDRFPSVKIVIGHLGEMTPGHLWRIDHWMIRHYKHKGLPMKHPFRYYFQKNIWLTTSGHFSTPDLLNAITAVGADRIMFSIDHPFEDIEEAAIWWDNLQMNPTDKVNMGRNNAIKLLKLDLPLDETAYESPVEIAWARCHATRPGQKVVAACCKSSHTGPPNVLSLPRPRAEASFLHGSLDPSSEFVSVVYPNERQARLAGTEVSHHPLSQRFVFSVDPTPEYIQSHLFTLNQEVRGVLPDVAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9H8</accession>
    <name>A0A8H6F9H8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004147</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149113.1">
        <property type="nucleotide sequence ID" value="XM_037295066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9H8"/>
    <dbReference type="GeneID" id="59332556"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052210">
        <property type="entry name" value="LysM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34997">
        <property type="entry name" value="AM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34997:SF1">
        <property type="entry name" value="PEPTIDOGLYCAN-BINDING LYSIN DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0843">Virulence</keyword>
    <feature type="domain" description="LysM" evidence="3">
        <location>
            <begin position="178"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="3">
        <location>
            <begin position="436"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="484" mass="51698" checksum="F3BEF545C584C164" modified="2022-01-19" version="1">MPSGCRIALSQNISCSPALVSPQFAAGGRALDPTTLGQYCTTLCYQSLQTFKTKVDAQCGDTNYQMFANSTLSQSGASIADPLAWAYNVICVQDSTGFCLPSLFNATKTLCSDCSLKYGVAMLGSDYGRNRIQPSAFSSLLSSCSIPASDYPYTYWPTPSTVNPFIPTSAPSTNCTGKKYTIKANDTCQSISQANSIATDRLIAANNIDYNCSSLIPGATLCLGPSCALQKVLQNQTCQDITTNQAFTFVQLIAWNPTIHLNCDNLDVMVGRSICISPPGSSVYKATLMNTSTVTISSMFPGPYVSGSINSNPPDATTSWYNTQTTFPPPVVTTFIANLTTASLVAARTSFCPITPDDFLNGLYEEDLPEDCDALIEPYCWPDPDAPTPSSTSFPASCTPFSGDSITTSNTPAVVTTSANPLPSPLEPGTIATCKKFHKVFTGETCYSIANNYKVSLDQFYTWNPSVGTTCAKLFLAFYVCVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9L0</accession>
    <name>A0A8H6F9L0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_003397</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149700.1">
        <property type="nucleotide sequence ID" value="XM_037294319.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331808"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="12">
        <location>
            <begin position="326"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="508"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="268"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="819"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="926"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1014"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1096"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1180"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1404"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="824"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="960"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1131"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1149"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1185"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1424"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1437"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="632"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="537"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="652"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="9">
        <location>
            <position position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6220265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1448" mass="159162" checksum="AF92473CC5A68A18" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9L1</accession>
    <name>A0A8H6F9L1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149792.1">
        <property type="nucleotide sequence ID" value="XM_037294411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9L1"/>
    <dbReference type="GeneID" id="59331900"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="461" mass="52401" checksum="17FC4141DD89FA93" modified="2022-01-19" version="1">MALEYLLVYCTSHGLTTIGEPPLPKTYQPVGESLLQTSRANKHSNLNSKDAMPVWIVLVFLTLATLKYCLSFVVWYVYQVISTVIIVADRRIRTIAAASAHKHPESHRARLDSLVQRMRVINRFKAAGAHRLRGVSVGEVRLDLKRRDTMKESGPAAKPSKSAVMNTWDRITSYACFRGFIGYLYMAVPQWYFGMICDFFLSGQPSELSLGYGTTSIILATMWGPGLATWTHYTITPPRKSINIQDHFPKGPSVLTDLWPLTASWLVAEHLTLSLPLALSRTFRLKHYAFDVDSWNTLDEYGQNKKIMQFALVFLLYLILVAVVAIPATMTMRRVHASMLSDDDLAIVPFHRGDRSRLHPYDKRSHIRHPGLTVAEAFNTITWDAYLRVLFVYFQYFAINQLVQLAYWSANWKLHQVLAVDKYASTNLPCSPVNKLLPFAERNASAEAYRGFFHPSPHSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9R2</accession>
    <name>A0A8H6F9R2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003684</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149294.1">
        <property type="nucleotide sequence ID" value="XM_037294606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9R2"/>
    <dbReference type="GeneID" id="59332095"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="5">
        <location>
            <begin position="70"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="6">
        <location>
            <begin position="440"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="774" mass="88617" checksum="17C112FF75BB23C9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA20</accession>
    <name>A0A8H6FA20_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003365</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149668.1">
        <property type="nucleotide sequence ID" value="XM_037294287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA20"/>
    <dbReference type="GeneID" id="59331776"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000007">
        <property type="entry name" value="Putative sybindin-like family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="142" mass="16236" checksum="4EA317EDD52543FD" modified="2022-01-19" version="1">MHRVVYSLIIINKAGGLVYNRDFNNALNKVSVNDLLVLAGTFHGVHAITKSLTPSSLGIPSSQRTGIEVLESSHFRLTSFQTFTGTKFLLFTEPHQPNVDVIIRKVYELYADYVTKNPFYQLEMPIRCEAFDRHLLTYMRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA46</accession>
    <name>A0A8H6FA46_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="5">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_003014</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150016.1">
        <property type="nucleotide sequence ID" value="XM_037293938.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331426"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000026">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000060">
        <property type="entry name" value="TBC domain protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <sequence length="1859" mass="206749" checksum="634B07D89428F099" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAE3</accession>
    <name>A0A8H6FAE3_9LECA</name>
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        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="15" type="ORF">HO133_002967</name>
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    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15">WasteWater1</strain>
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            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
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        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
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    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
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    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="entry name" value="MDH"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
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    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="31"/>
            <end position="174"/>
        </location>
    </feature>
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        <location>
            <begin position="176"/>
            <end position="341"/>
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    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="206"/>
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    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="36"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="146"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6220534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="345" mass="36291" checksum="6AB823085A820A98" modified="2022-01-19" version="1">MLASRTMLASRPILGACQRRAFSATVRQASKVTVLGGAGGIGQPLSLLMKLNPRVTELALYDIKGGPGVAADIGHINTKSVVTGYQPDADGLKQALTGAEIVLIPAGVPRKPGMTRDDLFNTNASIVRDLAKAVADAAPDANVLVIANPVNSTVPICAEIFKAKGTYNPKRLFGVTTLDVVRASRFISQHKSTDPADENITVVGGHSGATIVPLLSQSGYSLEGKDLDDFVFRVQFGGDEVVKAKDGAGSATLSMAMAGARFAESLLKAAQGQKGVVEPTFVDSPLYKDQGIDFFASKVELGPNGVEKIHDVGKITPKEQELLDVALKDLKKNIVKGVEFVKSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAG9</accession>
    <name>A0A8H6FAG9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HO133_002080</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">ATP-dependent RNA helicase associated with the nuclear pore complex and essential for mRNA export from the nucleus. May participate in a terminal step of mRNA export through the removal of proteins that accompany mRNA through the nucleopore complex. May also be involved in early transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Associates with the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150660.1">
        <property type="nucleotide sequence ID" value="XM_037293007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAG9"/>
    <dbReference type="GeneID" id="59330494"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17963">
        <property type="entry name" value="DEADc_DDX19_DDX25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000849">
        <property type="entry name" value="ATP-dependent RNA helicase DBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="4 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0811">Translocation</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="114"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="298"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="487"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF6221225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="510" mass="55863" checksum="2B86AB43AC82C3E5" modified="2022-01-19" version="1">MASLDDRISKPDNGQIEGKDIGSSTVNAKAEKEMSDVSKAQTDGATGSAGGSSGIYDPSYEVNVKLSDLQANPNDPLYSIKSFEELGLDENILKGIYQMRFTKPSKIQEKALPLLLRDPPTNMIGQSQSGTGKTAAFVLNMLSRLDYSPTMINTPQALVLAPSRELARQILGVIQVMGSFVEGLTAGVAIPMEVANRNRDGLKVQIVVGTPGTTMDMIKRRQLMADKIKILVLDEADNMLDQQGLGDQCIRVKQLLPKTVQVVLFSATFPNHVVEYASRFAPNANQITLKHEELTVEGIKQLYLDCPTELDKFDALVRLYGLMTIGSSIIFVRTRHTALEIERRMNAENHTVACLTGEYLGAQRDIIIDSFRSGHTKVLITTNVLSRGIDVQTVSLVVNYDLPDDGHGNTDPQTYLHRIGRTGRFGRVGVSISLICGQRSYRMLKEISDYFGVEMTSLPHNDWDQVEEIIKKVIKSSRAGSNFQATNAATAGPASGTNDVSMEQSGSSWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAV9</accession>
    <name>A0A8H6FAV9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150781.1">
        <property type="nucleotide sequence ID" value="XM_037293128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAV9"/>
    <dbReference type="GeneID" id="59330615"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014848">
        <property type="entry name" value="Rgp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12507">
        <property type="entry name" value="REDUCED GROWTH PHENOTYPE 1 RGP1, YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08737">
        <property type="entry name" value="Rgp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="820" mass="88925" checksum="27B1FC7517A7ADC0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAY2</accession>
    <name>A0A8H6FAY2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001673</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151140.1">
        <property type="nucleotide sequence ID" value="XM_037292601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAY2"/>
    <dbReference type="GeneID" id="59330087"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05367">
        <property type="entry name" value="SPR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000281">
        <property type="entry name" value="Uncharacterized oxidoreductase YIR035C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF8">
        <property type="entry name" value="BENZIL REDUCTASE ((S)-BENZOIN FORMING) IRC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="251" mass="27198" checksum="1EFED7D6E88AE946" modified="2022-01-19" version="1">MAKTVIVTGASKGIGAAIAEFLLKKSHNVVLIARTEQPLEELRTRYPEQVLVLAGDLGDISLAQKAVDSALTRFEALDGVIVNHGMMDPVTKIETSDIGEWKKLFDVNFFSAVAFAKAAIPILRKSHGCVVFTSSGVSTGAYSTWGAYGASKAAINHFARQLAVEEPKVTSVAVRPGVVDTDMQRQLREVHSAVMAAKDNDKFLGLHQAGKLLRPDQPGHVMARMVLDPPKELSGKYVDWESPELARYQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAZ5</accession>
    <name>A0A8H6FAZ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002815</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150569.1">
        <property type="nucleotide sequence ID" value="XM_037293740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAZ5"/>
    <dbReference type="GeneID" id="59331227"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000001">
        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018940">
        <property type="entry name" value="EF-1_beta_acid_region_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049720">
        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001326">
        <property type="entry name" value="Transl_elong_EF1B_B/D_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF21">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10587">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01182">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00825">
        <property type="entry name" value="EF1BD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation factor 1 beta central acidic region eukaryote" evidence="7">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="6">
        <location>
            <begin position="145"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="64"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="25510" checksum="E1B1BB4C0DA5F80E" modified="2022-01-19" version="1">MGFTDFVNDMGLQTLESWLRTRSYITGYGPSQADVATFKALKSTPEPAKYPHAYRWYKHMQSHESEFGSLPGDPSKSHTAYGPEQSELPTNPKAPTAVPAAEAEDDEDDLFGSDESEDEEAQKVKEANLAAYKKKKEGKVKPAAKSIVTLDVKPWDDETDMKALEKNMRSIEIDGLTWGASKLVPVGYGISKLQVNLVVEDEKVSIDDLQGQIEGDEDHVQSTDVAAMQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBB2</accession>
    <name>A0A8H6FBB2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosducin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001228</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosducin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151577.1">
        <property type="nucleotide sequence ID" value="XM_037292158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBB2"/>
    <dbReference type="GeneID" id="59329644"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02987">
        <property type="entry name" value="Phd_like_Phd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021514">
        <property type="entry name" value="DUF3176"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001200">
        <property type="entry name" value="Phosducin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024253">
        <property type="entry name" value="Phosducin_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394:SF5">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11374">
        <property type="entry name" value="DUF3176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02114">
        <property type="entry name" value="Phosducin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="606"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Phosducin" evidence="4">
        <location>
            <begin position="71"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="692" mass="76654" checksum="E532FF024B1CF8DA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC01</accession>
    <name>A0A8H6FC01_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001374</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151723.1">
        <property type="nucleotide sequence ID" value="XM_037292304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC01"/>
    <dbReference type="GeneID" id="59329790"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="36493" checksum="D1E0B460C65F08A9" modified="2022-01-19" version="1">MAQRQVSDDHALQFLASEGYLPLALSDHPGMVEAYTQLFAQTSKFFNLPPDSPEKTNHQAASGAAASEEGYSQIPHEKCIMTVKTSVHCPSTLRPELQTAWDLTGAFLASITTAIAHTLSLDPDVFSPFVAPCTAFPRTERTPTLVRMFRYDRPAAATAGPKVNAEKHKDLGLLSLVVGRSPGLQALDPTSNAWVSVEDEANLPPGAVARSGGLTATLLAGETLAFLSRGVYRAGVHRVVCAPSAENDAADPYRFSIVYTLRAAPAPLFTRDFESGVVGAFGPGERVEGESSAVMFERIMRTHWNVNAAPGVREEQRRKFEGRNGGGGVGVENRQERASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC28</accession>
    <name>A0A8H6FC28_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001464</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151813.1">
        <property type="nucleotide sequence ID" value="XM_037292394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC28"/>
    <dbReference type="GeneID" id="59329880"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF22">
        <property type="entry name" value="CAPSULE ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="2">
        <location>
            <begin position="321"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="657" mass="76139" checksum="A95415FB08ECA337" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCS1</accession>
    <name>A0A8H6FCS1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GTPase-activating protein GYP7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">GAP for YPT7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000123</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152498.1">
        <property type="nucleotide sequence ID" value="XM_037291063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCS1"/>
    <dbReference type="GeneID" id="59328542"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000032">
        <property type="entry name" value="GTPase activating protein (Gyp7)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000005">
        <property type="entry name" value="TBC1 domain family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021935">
        <property type="entry name" value="SGSM1/2_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF502">
        <property type="entry name" value="SMALL G PROTEIN SIGNALING MODULATOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12068">
        <property type="entry name" value="PH_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="5">
        <location>
            <begin position="446"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="743"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="789"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00072091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00082648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="829" mass="93659" checksum="B7B89C63E049E869" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCS3</accession>
    <name>A0A8H6FCS3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152934.1">
        <property type="nucleotide sequence ID" value="XM_037291499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCS3"/>
    <dbReference type="GeneID" id="59328979"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="95" mass="10203" checksum="8B05C8B82F51CF14" modified="2022-01-19" version="1">MTMQASPIYLQEQTISAATDALLSANARTVELEHQVQALHAFIDANGLMRVAPQSIRQHMLTGPGAAKTLGNQIKVAACKVSDKLRTKTSDAKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD64</accession>
    <name>A0A8H6FD64_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010574</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153060.1">
        <property type="nucleotide sequence ID" value="XM_037301426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD64"/>
    <dbReference type="GeneID" id="59338964"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="4">
        <location>
            <begin position="277"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="45"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="224"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="130"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="379" mass="42658" checksum="9D3019FC888A9476" modified="2022-01-19" version="1">MADQQTMPLRDRYGEWISEAFEKKEGDMDAVKEHRLEEANRLARGALNLGKERHSSRTFRGLQKVDNGSKRTPSELRTGSTKPGSGGSVPLLTPPRSPTKSGVSTGTSVAITPVVEITDSQEVADIRNSLRNHEYEILSLRMQLEDIRAEVQDLHKQCRKASEDYANAVCRDDEENLKRFEKREEKLKKLLEQKNQAIDGLQEEIVEKKRCEGELKEKLAEVEGRDKAREAMPTQVEKTVSDGSDTVEKGNRKGKDTSVRAKQRAIGSWEFRRKKGENETPLSAGMAGTGKSTIAQTIARTLTKQKRLAANFIFSRGRGDLSDTGKFFSIVAIQLAATSRRLKHYICEAIARNEGISRQSMRDQWTKLVYQPLLKLGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD91</accession>
    <name>A0A8H6FD91_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010802</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153287.1">
        <property type="nucleotide sequence ID" value="XM_037301653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD91"/>
    <dbReference type="GeneID" id="59339192"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="48749" checksum="62D974588E22C065" modified="2022-01-19" version="1">MALLWELPNEMLVMIVRFLVGRRINHFDPVATKDLQSLRLVSHRFKTVATPILFKNVVLDFSSFKLADMKRIHELVIAPLEAQWEDEIPNFDAIRKILRETMAVLPTLYHVENSVSTHEPERTAMAYQKIWDEMGIDLSGNFPTVTNDQSDTLGRGVSYLMTSLTISITSLKLREVPFENWLYHWRIPDQVVHLDIELRISQEIGTLEDPVQTCFAVQDWRKQFTALKRLKTLRLSLSCEGLYPNEDEWFEMGGPHLYFDNLLTGPELSVDRLEAATCELEYRKHKGISEAGVINDCTFPRLESLSLINCPLREEGLLYLAVMHQRTLRSIQLHRVVFDATPGQMPVKELAKIRNYLPNLTHLVLSKIDLYDPDRGHFDLDLEMREEVASVYRWDKGDGGGDDGLVRYAWDLGRIVEGELGPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDB5</accession>
    <name>A0A8H6FDB5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152886.1">
        <property type="nucleotide sequence ID" value="XM_037291451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDB5"/>
    <dbReference type="GeneID" id="59328931"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="775"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="636"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="726"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="785"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="804" mass="88567" checksum="CD7959DF6D0744A1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEI4</accession>
    <name>A0A8H6FEI4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartate transaminase</fullName>
            <ecNumber evidence="4">2.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Transaminase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010122</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153795.1">
        <property type="nucleotide sequence ID" value="XM_037300978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEI4"/>
    <dbReference type="GeneID" id="59338514"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000064">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF55">
        <property type="entry name" value="GLUTAMATE OXALOACETATE TRANSAMINASE 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="61"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="443" mass="49095" checksum="A882913B78AB9A93" modified="2022-01-19" version="1">MSPNSTSSQGNKPLTRLSNIKGHINMEGCNPTVFHDDIVPLAPEDPLFGLMAAYKKDDFDKKVDLGIGAYRDNNAQPWVLPVVKKAEELLRKDPKLNHEYLPIAGLADFTAAAQKLILGADSPAIEDKRVVSLQTISGTGAVHLGGLFLAHFYPRNPKQLYFSSPTWANHNQIFSNVKLPTKSYPYFSPLTKGLDFDGMRSTIEKAPEGSIILLHACAHNPTGVDPTRDQWKEIAKVIRAKNHFPFFDCAYQGFASGDLENDAWAVRYFVSEGFELCIAQSFAKNFGLYAERTGVFHFVTGAGPDAQRTIARIGSQLTLLQRSEISNPPAYGARVASLVLNDPELFKEWQANLRTMAGRIIDMRKALRSKLEELNGKEGSWKHITDQIGMFSFTGLTEKQAVKVKDDAHVYMTKNGRISMAGLNTGNVDYTAKAIDKVVRETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGT3</accession>
    <name>A0A8H6FGT3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155941.1">
        <property type="nucleotide sequence ID" value="XM_037298605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGT3"/>
    <dbReference type="GeneID" id="59336132"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="60901" checksum="60D12D321309B5DC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGW2</accession>
    <name>A0A8H6FGW2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EXS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008818</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155682.1">
        <property type="nucleotide sequence ID" value="XM_037299681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGW2"/>
    <dbReference type="GeneID" id="59337213"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF46">
        <property type="entry name" value="PROTEIN ERD1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="6">
        <location>
            <begin position="190"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="397" mass="45427" checksum="6793B5B7DF5FE179" modified="2022-01-19" version="1">MDGDPAVEPELDAFSLLLPLPYRIAVILVLGVWAWGVNLHYLSILKIDLPILIKYHTRTSSSIPPHHRRTYQLAATVTVPLIISILLFWIITHGQPALVLAWDILPQSFLFLLAVFFLLPFQRLSRTGRYRFLSTLKRVSIGGIAEADNGKFGDILLADVLTSYAKVLGDLFVSWCMLLSPGLSSTGKPDRNCGGAYMVPLIISVPSMIRLRQCLIEFLRVRNNPNKNATTGWGGQHLANALKYSSAFPVIVLSALQRGTNPNDFGMTEASLFRLWLFFVFLNSFYSFYWDVAKDWDLTLFAPSSVRNNPEHPWGLRRHQHFHSKQMYYGAILIDLMLRCTWSFKLSPHLDHFNDLEGSIFIMEFLEVLRRWLWIFFRVETEWGILIHEPSEAAKNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGX1</accession>
    <name>A0A8H6FGX1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome biogenesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008892</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in ribosomal large subunit assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RRS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155756.1">
        <property type="nucleotide sequence ID" value="XM_037299755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGX1"/>
    <dbReference type="GeneID" id="59337287"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007023">
        <property type="entry name" value="Ribosom_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04939">
        <property type="entry name" value="RRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="155"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="220" mass="23517" checksum="9FDD32D309CC8BAD" modified="2022-01-19" version="1">MPGDSTPAASQAASTSALKSAPSAASHPDPSSITVTKAIPPTFDLGNLLCHDPTPLPPLPPPALLAATARDCAQSLINQLLTACPVVTAAAGGGVLMALPPATFPLPREKPLPRKKAETTWERFAKKKGIRDRKRGEGGREYDAGKGEWVPRWGYKGRNKDGEGGWIVEVDEEKERGLEGEGTGKTVRGEGRRDRVEKVRRNERKQRANERKGRKSGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHA8</accession>
    <name>A0A8H6FHA8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156194.1">
        <property type="nucleotide sequence ID" value="XM_037298858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHA8"/>
    <dbReference type="GeneID" id="59336387"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="67" mass="6887" checksum="0B805E767DD4176F" modified="2022-01-19" version="1">MPDSKEQAQAAEQEAQNAKDPSHPAHPQHPKHGEWLKNAGKKFGNAAIFGAGATAGSDAIQGAISNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FID3</accession>
    <name>A0A8H6FID3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ustYa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156713.1">
        <property type="nucleotide sequence ID" value="XM_037298078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FID3"/>
    <dbReference type="GeneID" id="59335585"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021765">
        <property type="entry name" value="UstYa-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33365:SF4">
        <property type="entry name" value="CYCLOCHLOROTINE BIOSYNTHESIS PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33365">
        <property type="entry name" value="YALI0B05434P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11807">
        <property type="entry name" value="UstYa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00035112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="23677" checksum="23BC4989A88095B3" modified="2022-01-19" version="1">MSIAKHASGYPRTTQELYNSSKYAGHPLDPNVDEAWRLLLRPMDIRVTAAELARNNRTSVQLPRDRGYLAWLGVFHELHCLTGLKFAYKIMQKLLRQWKYKEQYFPNMTSAQAYDMDSHTGMMPTTTRNMSRTYEILDHCLDRIRATIICHPDIASLTTFFWGSGTKPTVDGTKTLHSCVDWDVLMDSIMGRAVSSEEVDTLVNPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIF1</accession>
    <name>A0A8H6FIF1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007245</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156772.1">
        <property type="nucleotide sequence ID" value="XM_037298137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIF1"/>
    <dbReference type="GeneID" id="59335644"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13636" checksum="AA436F0A47EB655E" modified="2022-01-19" version="1">MGKSDQYFPNAELWDPHRWESMADPKDGEREKMDYGYGLLSTGADSTYLPFGAGRHRCIGEQFAYLQLSIVLIVMVREFRLRNEDAWNLISEDYWRNRLAGFSSFQISHDNYRDAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FII0</accession>
    <name>A0A8H6FII0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156832.1">
        <property type="nucleotide sequence ID" value="XM_037298197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FII0"/>
    <dbReference type="GeneID" id="59335705"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000019">
        <property type="entry name" value="Thiamin pyrophosphokinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031804">
        <property type="entry name" value="DUF4743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15916">
        <property type="entry name" value="DUF4743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="136"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="316" mass="35926" checksum="801ECCE49C041193" modified="2022-01-19" version="1">MKTGLDLVNECDNFPYYESDPEEYSRLRSTSWELHLEGGSGPYGYIKQSVVEKMPWDHDAWQIDDEKKCITPAPKRYTHTQETVIRETLEVAKTRDIFQILRGWRNELYPVHGPYSSRKPMVSMERAGSALFGINTYGVHMTAYVNTPQGMKIWVPRRARNKQTYGGMLDNTVAGGLSTGEKPFECVVREAAEEASFSEQLVRSNAKPCGTVSYFHIRDARAGGEAGLLQPECQYVFDMEIGAEVIPKPGDNEVEEFHLWTVDEVKRALAEGQFKPNCAAVLLDFFIRRGILTAENEKDYTEIVSRLHRKLPFPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIJ6</accession>
    <name>A0A8H6FIJ6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156779.1">
        <property type="nucleotide sequence ID" value="XM_037298144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIJ6"/>
    <dbReference type="GeneID" id="59335651"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="185" mass="19522" checksum="36B39C8BAC4B3B00" modified="2022-01-19" version="1">MRSLKVLSRILTSATQTAAAALPNYIGNAVILFIGFISVQAPKFHYAKSLASKLYKDSYAIVDTQDQATLLKGWLAYSALTANGGGPYLASLSETVFCTRDSGYDGYAGIAGNTPADGHQYCNSCLTEGINSGADDIWCQVNDRADGGTATLEDHMYHGGNPKKGPSGMCTVDTQCAGRGWMVGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIZ6</accession>
    <name>A0A8H6FIZ6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007503</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157029.1">
        <property type="nucleotide sequence ID" value="XM_037298394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIZ6"/>
    <dbReference type="GeneID" id="59335902"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="261" mass="29135" checksum="39F84E4373D9458C" modified="2022-01-19" version="1">MSGSTLEITAIASVTAGPYPKLPDVVECAPEYGENLDKHACYKALQTMPDGRRFSQFVSRKRYHGQEALITPVYYYDNNMNASCIIDNFGRGGFITYKLENTIEGVSKYKKVPAGYNTPLFLTVQVSPRQGRGRPGDTDPRVNEAVQNGLLESIPPIVFKVNRAKIFVIVNALKEIYKRMRIGGTQAWWDSRNITNSIPENVDYNCYGSEGSPRPRDCEDASFTFMGEGNVIVGPKEPLIKQVGMKTNDILLLLPKSNLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJY8</accession>
    <name>A0A8H6FJY8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myosin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Class I unconventional myosin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Type I myosin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_004224</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157144.1">
        <property type="nucleotide sequence ID" value="XM_037295143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJY8"/>
    <dbReference type="GeneID" id="59332633"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001411">
        <property type="term" value="C:hyphal tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11858">
        <property type="entry name" value="SH3_Myosin-I_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000015">
        <property type="entry name" value="Myosin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035535">
        <property type="entry name" value="Fungal_myosin-I_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054489">
        <property type="entry name" value="Myo1_CA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF837">
        <property type="entry name" value="MYOSIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22773">
        <property type="entry name" value="Myo1_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 18" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11 18" id="KW-0518">Myosin</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 17" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="21">
        <location>
            <begin position="41"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="22">
        <location>
            <begin position="779"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="1083"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="18">
        <location>
            <begin position="593"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="953"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1128"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="960"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1022"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1036"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="1071"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1129"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="1146"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1238"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="134"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAF6229887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1263" mass="139349" checksum="1B118E078F4F06AA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK02</accession>
    <name>A0A8H6FK02_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="15">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="15">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ALA1</name>
        <name evidence="17" type="ORF">HO133_004314</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 15">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK02"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070143">
        <property type="term" value="P:mitochondrial alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.40:FF:000003">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000011">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059090">
        <property type="entry name" value="ALA1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26023">
        <property type="entry name" value="ALA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 15" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="16">
        <location>
            <begin position="8"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="605"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="720"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="724"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6229976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="956" mass="107488" checksum="915C25A59C85C1C6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKJ7</accession>
    <name>A0A8H6FKJ7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycerophosphocholine acyltransferase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_004568</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157485.1">
        <property type="nucleotide sequence ID" value="XM_037295484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKJ7"/>
    <dbReference type="GeneID" id="59332975"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021261">
        <property type="entry name" value="GPCAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201">
        <property type="entry name" value="OS01G0585100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10998">
        <property type="entry name" value="DUF2838"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="434"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="30"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="499"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6230228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="569" mass="65030" checksum="5E6BCBBE4B88A21C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKS1</accession>
    <name>A0A8H6FKS1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004638</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157555.1">
        <property type="nucleotide sequence ID" value="XM_037295554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKS1"/>
    <dbReference type="GeneID" id="59333045"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140575">
        <property type="term" value="F:transmembrane monodehydroascorbate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045150">
        <property type="entry name" value="CYB561D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422:SF24">
        <property type="entry name" value="DOMON RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422">
        <property type="entry name" value="OS05G0565100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="13">
        <location>
            <begin position="33"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="288"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="363"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="489"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="635"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="549"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="563"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="636"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="647"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="675"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="688"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="728"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="755"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="817"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6230298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="836" mass="91261" checksum="BD69156A0C07124A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKS6</accession>
    <name>A0A8H6FKS6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dolichol phosphate-mannose biosynthesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_004653</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of the dolichol-phosphate mannose (DPM) synthase complex; essential for the ER localization.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DPM2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157570.1">
        <property type="nucleotide sequence ID" value="XM_037295569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKS6"/>
    <dbReference type="GeneID" id="59333060"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033185">
        <property type="term" value="C:dolichol-phosphate-mannose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180047">
        <property type="term" value="P:dolichol phosphate mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009914">
        <property type="entry name" value="DPM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15039">
        <property type="entry name" value="DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15039:SF11">
        <property type="entry name" value="DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07297">
        <property type="entry name" value="DPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6230313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="91" mass="10153" checksum="E474D7FD853F3515" modified="2022-01-19" version="1">MLDRLVGLAMLIVATTVFLYYTTWTLLMPFVDQGHPLHDFFPPRVWAIRIPVILILLASAVVGSFLSVVMIRSNRKKTAKARAAEAGKKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKU6</accession>
    <name>A0A8H6FKU6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157634.1">
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        <location>
            <begin position="538"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="895" mass="96027" checksum="5D38F7EE69558504" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZV2</accession>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
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    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        </location>
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        </location>
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        </location>
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        </location>
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            <end position="711"/>
        </location>
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        <location>
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            <end position="768"/>
        </location>
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        <location>
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            <end position="912"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6230340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="912" mass="100047" checksum="3A4DBD760A4313ED" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C4X2</accession>
    <name>A0A8H6C4X2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin 3 binding protein But2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000029">
        <property type="protein sequence ID" value="KAF6217405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146840.1">
        <property type="nucleotide sequence ID" value="XM_037297817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C4X2"/>
    <dbReference type="GeneID" id="59335321"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034214852" description="Ubiquitin 3 binding protein But2 C-terminal domain-containing protein" evidence="2">
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            <begin position="22"/>
            <end position="221"/>
        </location>
    </feature>
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        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="24019" checksum="12026A66BF000DA6" modified="2022-01-19" version="1">MHFSLTTTLPLLSTLLSLTTAQQSTDANGCSTFEPTSAFQIYSDLPDLSVDLTIPNYSNLTNGTTNSTRRLSSRSKARSRPKSRRSAHIPFTFYVSQDAGNTNQQDLVVAFAGLPCGGPGPFSFEFNFQPQSAYFAQGQGQINMFRVDTLALGDAPTWNDIEPLTGSLVGTFELPAVGSEEPALLFLNQLVCQDQLVLRFGISRYSDEGGVWRTSMPGGWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8H6C6A6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Catalase</fullName>
        </recommendedName>
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    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
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            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <dbReference type="GeneID" id="59334935"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
    <dbReference type="GO" id="GO:0020037">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR36195:SF4">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G01990)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36195">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G01990)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="43570" checksum="B00673523A212091" modified="2022-01-19" version="1">MDISNKGEDQAAMANRKYIHWQDEGVEKIPPNEAEDIQAVADQINAMQKAQYNSHRHCYTGTHARTQGIVKGMLIVKDDLPKHLKQSMFEHGGQHAVVCRYSSEPGDPGLDDRIPQPRGFAMKVFNVPGEKFDPTKTAQDIEFNSTPALDLADAKTTREIIDLRIKYGGDKPELYKKLEARPDTDLQKFRDSVRNTHLESTRQYSQTAYRFGDYVAKYCLVPSSETQKKLYEETVKPDEHEPDILSQWLQNFHEAHDAEYLLQFQLCENLEDQPVEYAGKVWDPEKYPWQTVATLVIPKQDSFNYELKSFWEDHMRIDPWLGLKSLQPLGSPNRLRRVVYPASSKLRRMMNGKKEVHVHEMDQIPGPSPAVGGNFLKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6B3</accession>
    <name>A0A8H6C6B3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147162.1">
        <property type="nucleotide sequence ID" value="XM_037297452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6B3"/>
    <dbReference type="GeneID" id="59334955"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="287" mass="32815" checksum="8B07EFDD54C02358" modified="2022-01-19" version="1">MSDRTADQVIRIEDSQEHSQQVIMIPGTDEQTSGSSDSEEFIQESNTEEAIIMSPESEEKMHGAGRDEKVVMIPDGEEEVDSIREAIVDFARWLITSSRRPNSKAHSIFDYPWLGPPTNNVWRPWEDQSRINSTLGGIPHAVTAAYLGERQVKRSKTDCRHRVRLHTDRAAGGKILPTCWTGHGRKISLKDLGGDEWDELALFRWNQRGLSPRVFCDERTSWGLEDCLKQMGAFDRDVLKFAVERIMKKRVQRKREAKARMRMRHSQGWYISDAGDPSPLLSPVAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6I0</accession>
    <name>A0A8H6C6I0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147340.1">
        <property type="nucleotide sequence ID" value="XM_037297215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6I0"/>
    <dbReference type="GeneID" id="59334718"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="285" mass="31708" checksum="B132BD6EEB9C0D9F" modified="2022-01-19" version="1">MDTGGQGRQGPSWGGMEGLLVQRRRASRILSLQHFNHAGWWKGVRIEVAAKDVRNRLLSTVLTYTSVLKERAWKLMANADRIARLTQAANAIGQRAVDRLAEPEGEKGTIEGEEVRVNTDQDKGYEADFDEFGFLSADQAMLRHSHLAKREKKSVRKRAMRWHFCRPNRNGWDDYLIGQRNAALRATEPEVDLAEASISEMEVSSSESGNSSAESLHLVTPMVRFLVSDAEDASSRSPDSPMQEAPLETADDPPLEEDESLDSWSDPDETLATIGDSVGLAVRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6L4</accession>
    <name>A0A8H6C6L4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">carnosine N-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006723</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carnosine N-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147056.1">
        <property type="nucleotide sequence ID" value="XM_037297620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6L4"/>
    <dbReference type="GeneID" id="59335124"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030735">
        <property type="term" value="F:carnosine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012901">
        <property type="entry name" value="CARME"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303:SF6">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07942">
        <property type="entry name" value="CARME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01296">
        <property type="entry name" value="N2227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="333"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6217621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="410" mass="46287" checksum="14CB3FAA887A2170" modified="2022-01-19" version="1">MAHESEVPSPFSDPEERRVLFAALDSFRKITLPSTKLIHWQMLAAPPFNLLSVFDHVDDAIDANAEIGSEMMNTALLSFGIDNLKSTTGLETPASSSFGSADENEAQRLIDWRNTAKPPDIDKARSTIRQLYRDWSHEGAAERKACYEPVIQDVVRAFWHSPSKHDVKILVPGAGLGRLVFELCRRGYTVEGNEISYHQLIASNWVLNHTVKGQQFDLYPFALDFSNVVSREHQLKKVKVPDVHPGSALEDVFGHTTANAADRMSMTAADFVVLYGDEEHKDMFNAVVTVFFIDTAPNLIRYIETIRNCLQDGGLWINLGPLLWHFADRAPSEEDHSKRAEGPREKTGIEEPGAFELTDEEVLILVEKMGFDIEKREIRNGGVGYIQNPESMLQNVYRTSHWIAKKRPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Q8</accession>
    <name>A0A8H6C6Q8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. Reversibly leaves the enzyme after glucose depletion, causing the catalytic subcomplex V1 to detach from the V0 section.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase C subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147258.1">
        <property type="nucleotide sequence ID" value="XM_037297548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Q8"/>
    <dbReference type="GeneID" id="59335051"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14785">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.10">
        <property type="entry name" value="subunit c (vma5p) of the yeast v-atpase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1180">
        <property type="entry name" value="Vacuolar atp synthase subunit c, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004907">
        <property type="entry name" value="ATPase_V1-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036132">
        <property type="entry name" value="Vac_ATP_synth_c_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03223">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118203">
        <property type="entry name" value="Vacuolar ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6217823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="394" mass="44182" checksum="3F1CE40641251981" modified="2022-01-19" version="1">MSKGLKYLLVSLPTSISPSNDHDEALTALRSAVTTDYATVFPFQIPEFKIGTLDALVQQADDLAKLAQGCEAVVGKVGDSLRNILEGDEEKISQQKTVNDKPAEQYLKSFHWNKVKYRADKPLGEVVVTLQKEISSIDNDVKNKYNQYNQVKTSLTTLQRKQTGNLSNKSLTAIVDPSLVIQNSEYLETHLIAVPNTITKDFLKTYETISPMIVPRSAQLIDADDEFTLYAVTTFKKHSSEFVHKCRESKWTPRDFKYKEGGKEEEQKEVERVGKDERKVWGEALRLGRTAWGEAVMAWVHVLALRVFVETVLRYGLPLQFVCGLLRTTPKLAKKARSSLDVSYSYLAGNAFGRDKKGRVAKDDSATSADMQAAGHMGNDGDYAAYVCYEFEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C700</accession>
    <name>A0A8H6C700_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147563.1">
        <property type="nucleotide sequence ID" value="XM_037296989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C700"/>
    <dbReference type="GeneID" id="59334491"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034801540" evidence="1">
        <location>
            <begin position="20"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="351" mass="35753" checksum="EF1F549428D9C4EB" modified="2022-01-19" version="1">MRPFTALSLLLALSGSAFSAPAPENDLKIRNVDECKAVTVIISVLSQYKASATSFCSSFISIPVKTVTSATTTTSTPKPSTVTSTITVTPKVSVTATTTTTPTVIYSITSAPAKRAISPKLAERNINNQPIPSYVSAFASSEISSACSCLSISPSTTTVKTTSTVVGPAPTIIVKTTITASTVTTTISTVTVTASSTVTECANALPTFILKLASPVVLNGAQLEGTYDQIDDGDGELVGFNGLTDPSAAGVLSLTSTGFLMIDSVGQIAFRIPGQTGLQLLLFDPPAEVEASDTPVVCSVATGALTCSDGVNSVLQLCPGFAVTDDLFIGPVVASGCVGVVLDVLPVCIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C776</accession>
    <name>A0A8H6C776_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein CASP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_006475</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CASP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6218063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147498.1">
        <property type="nucleotide sequence ID" value="XM_037297373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C776"/>
    <dbReference type="GeneID" id="59334876"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012955">
        <property type="entry name" value="CASP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057476">
        <property type="entry name" value="Cux_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043">
        <property type="entry name" value="CCAAT DISPLACEMENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08172">
        <property type="entry name" value="CASP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25398">
        <property type="entry name" value="CUX1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="711"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="Cux N-terminal" evidence="14">
        <location>
            <begin position="45"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="CASP C-terminal" evidence="13">
        <location>
            <begin position="467"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="443"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="571"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6218063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="776" mass="86057" checksum="5B6ABD7F4045FCBB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7L8</accession>
    <name>A0A8H6C7L8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005807</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147893.1">
        <property type="nucleotide sequence ID" value="XM_037296712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7L8"/>
    <dbReference type="GeneID" id="59334212"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051414">
        <property type="entry name" value="Adenylate-forming_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439:SF2">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439">
        <property type="entry name" value="PHENYLACETATE-COENZYME A LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="3">
        <location>
            <begin position="406"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="734" mass="81716" checksum="6798ADD5E4138D84" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8K6</accession>
    <name>A0A8H6C8K6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148327.1">
        <property type="nucleotide sequence ID" value="XM_037296345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8K6"/>
    <dbReference type="GeneID" id="59333841"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="758"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="983"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1013" mass="116560" checksum="07F12FD9723AD466" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8S4</accession>
    <name>A0A8H6C8S4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148467.1">
        <property type="nucleotide sequence ID" value="XM_037296485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8S4"/>
    <dbReference type="GeneID" id="59333982"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="646"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="762" mass="83052" checksum="7E5B3F2483CB0B5B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8U1</accession>
    <name>A0A8H6C8U1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_005524</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. TRA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148415.1">
        <property type="nucleotide sequence ID" value="XM_037296433.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333930"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05163">
        <property type="entry name" value="PIKK_TRRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046807">
        <property type="entry name" value="Tra1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046805">
        <property type="entry name" value="Tra1_ring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF1">
        <property type="entry name" value="TRANSFORMATION_TRANSCRIPTION DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20175">
        <property type="entry name" value="Tra1_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20206">
        <property type="entry name" value="Tra1_ring"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAT" evidence="4">
        <location>
            <begin position="2646"/>
            <end position="3201"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="3">
        <location>
            <begin position="3458"/>
            <end position="3783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2010"/>
            <end position="2054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3205"/>
            <end position="3289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2033"/>
            <end position="2050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3205"/>
            <end position="3217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="3236"/>
            <end position="3250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6218980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3812" mass="434479" checksum="01B0DE039A7E6C5E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9W3</accession>
    <name>A0A8H6C9W3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SprT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148781.1">
        <property type="nucleotide sequence ID" value="XM_037296083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9W3"/>
    <dbReference type="GeneID" id="59333577"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006640">
        <property type="entry name" value="SprT-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035240">
        <property type="entry name" value="SprT_Zn_ribbon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23099:SF0">
        <property type="entry name" value="GERM CELL NUCLEAR ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23099">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10263">
        <property type="entry name" value="SprT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17283">
        <property type="entry name" value="Zn_ribbon_SprT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00731">
        <property type="entry name" value="SprT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SprT-like" evidence="2">
        <location>
            <begin position="392"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="672" mass="75146" checksum="35CFCA5962FE27F7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA73</accession>
    <name>A0A8H6CA73_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003633</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149243.1">
        <property type="nucleotide sequence ID" value="XM_037294555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA73"/>
    <dbReference type="GeneID" id="59332044"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14480">
        <property type="entry name" value="SPX_VTC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.100.30:FF:000002">
        <property type="entry name" value="Vacuolar transporter chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF2">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 3 COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="644"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="674"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="712"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="542"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="566"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="773" mass="88261" checksum="A267684F07F6A885" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAB7</accession>
    <name>A0A8H6CAB7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Core protein II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_003682</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149292.1">
        <property type="nucleotide sequence ID" value="XM_037294604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAB7"/>
    <dbReference type="GeneID" id="59332093"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000021">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000039">
        <property type="entry name" value="Ubiquinol-cytochrome c reductase core subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF209">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="12">
        <location>
            <begin position="47"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="13">
        <location>
            <begin position="199"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6219857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="460" mass="49750" checksum="4C1EB99DF357A754" modified="2022-01-19" version="1">MIPRAALARNGQRALQWQMRSNNWRCLATAASGSFSYEIGDAGGVKVASRDLPGPTTHLAVVAKAGTRYQPLPGFSEGLEKFSFKNTWKRSALRITRETELLGGEYSSYHSRENLVVGAKFLRDDLPYFAELIGEVISQTKYTAHELHEEVEPNMNFSRKELLGNPLEFAMNSAHGVAFHRGLGNSLHPSSSTPMTKYLNENRLQQYSRVAYAKSNFAVVANGADHQELSKWINEFFTELDQSPPQDFPSTGTESTKYYGGEERIAHDSGNTMVIAFPGSGSATGSSYKPESQVLAALLGGQSNIKWTPGFSLLSKATAAYPQAHTATAHHTYSDAGLLTVSMYGNADQIRQASQEVVKAIKSVAAGNVGREEIKKAVAAAKFKALESGQNMNTGIELTGAGLVQGGKAFQIDELGKSIDTVTEDQVKTVAKSLLDGRATVSTVGDLFILPFAEEIGLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAC7</accession>
    <name>A0A8H6CAC7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">XRCC4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149307.1">
        <property type="nucleotide sequence ID" value="XM_037294619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAC7"/>
    <dbReference type="GeneID" id="59332108"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42067:SF1">
        <property type="entry name" value="MITOTIC APPARATUS PROTEIN P62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42067">
        <property type="entry name" value="YALI0C15378P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58022">
        <property type="entry name" value="XRCC4, C-terminal oligomerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="210"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="281"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="337"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="41187" checksum="FEF6ED4FFDEC3036" modified="2022-01-19" version="1">MATEHILYLLRSDSDGDHVLINVSSEGPSPLDLKLLATEGESPYIATIRQSRISKLRDKKNQLSDDQWENVLHSTLLQRRIQGSEASTLEKLEVHATLAGDQLSIIFRNNFSGITQKLGEVAFKKDESQELDITRWTGTAVERSYRLDREVRDLASKYDEQSKKMEKLNNQLEDLIKAKKEHEDSLLQTFRELLNTKKLKIRDQQRTLASAKFDPKQAAKLQNARSTSKRRAATASRAGKRKAMSGHGASDSSEESGFKAQASKPKPESDVSEQINTPEQSDQDVTEDESGDDPDSAPQAARLPDKSKAATGAKGAIGEEMQLDTLPPMRDLPFGEGGRREQVKRPVTEDNLTLTQEAGNEGEETDDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAJ2</accession>
    <name>A0A8H6CAJ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Orc1-like AAA ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_003787</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149397.1">
        <property type="nucleotide sequence ID" value="XM_037294709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAJ2"/>
    <dbReference type="GeneID" id="59332198"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020796">
        <property type="entry name" value="ORC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047088">
        <property type="entry name" value="ORC5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048866">
        <property type="entry name" value="ORC5_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14630">
        <property type="entry name" value="ORC5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21639">
        <property type="entry name" value="ORC5_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="8">
        <location>
            <begin position="15"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="ORC5 lid" evidence="10">
        <location>
            <begin position="224"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 5 C-terminal" evidence="9">
        <location>
            <begin position="312"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6219962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="50004" checksum="E5E06B348FF0AAC9" modified="2022-01-19" version="1">MLPEDLSLTLLDQAPCRALQIRQILALLSPNLPSPSTILLHGVEATGKSLTIKAVLEAIDTPSAIVRSQECITTRHLLERTIAVVKEALAAHGHLEEAHGLDGRCENISAFRVELQHLLEGKEKFILVFNGIDRQCEAAPTLLPAIARLGEILPNLTAILITTTSHPHLLHHACIPHIHFPPYTRTETLSILSRTPLLLHASSASSQSLNTTTAANDDDTEWLWTRFIAAVWDSLGQTAARSIPDFRDVCARLWPPFIQPIVDGHYGAREFSKLLVKNRSLFQSEDALIDTIVPTTPSSTISKPKKQFSYTLPYYPTHLLISAYLASHNPPKHDITLFSKTSLSKRRKRGGGTALTSNRASKHRKVSRRLLGPHPFPLERLCAIFQAILPHTYTGGGADMMCQVATLVGLRLIVKAGMGGDVLEGGGKWRVNVGWGFVRGVARGVNFDVESYLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAM0</accession>
    <name>A0A8H6CAM0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003546</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149156.1">
        <property type="nucleotide sequence ID" value="XM_037294468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAM0"/>
    <dbReference type="GeneID" id="59331957"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="157" mass="17171" checksum="B98588EE5E72A08C" modified="2022-01-19" version="1">MSIPSDYLLVATHLAHSPSLLEAFVHESLLRLTNISIPVTIDDVTRNLPTSPPTTVPEDVVAILIRQRALPATIGVNGVEAGYVVTETASSTAEVFWVLFVNFGPGRACTVEGAVEVRYFYPRDADARAMFLAAYYDDSVIAFRVIEIANDSQRVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAU9</accession>
    <name>A0A8H6CAU9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003704</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149314.1">
        <property type="nucleotide sequence ID" value="XM_037294626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAU9"/>
    <dbReference type="GeneID" id="59332115"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01736">
        <property type="entry name" value="LSm14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025761">
        <property type="entry name" value="FFD_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025609">
        <property type="entry name" value="Lsm14-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025768">
        <property type="entry name" value="TFG_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586">
        <property type="entry name" value="SCD6 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586:SF0">
        <property type="entry name" value="TRAILER HITCH, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12701">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01271">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51513">
        <property type="entry name" value="FFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51536">
        <property type="entry name" value="TFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="7">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="DFDF" evidence="4">
        <location>
            <begin position="450"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="FFD box profile" evidence="5">
        <location>
            <begin position="520"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="TFG box profile" evidence="6">
        <location>
            <begin position="548"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="77"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="336"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="413"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="485"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="532"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="FFD box" evidence="1">
        <location>
            <begin position="520"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="TFG box" evidence="2">
        <location>
            <begin position="548"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="122"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="172"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="261"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="445"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="535"/>
            <end position="561"/>
        </location>
    </feature>
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        <location>
            <begin position="577"/>
            <end position="587"/>
        </location>
    </feature>
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        <location>
            <begin position="588"/>
            <end position="601"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00846"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6219879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="609" mass="65096" checksum="29761C0D57D16DF3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB26</accession>
    <name>A0A8H6CB26_9LECA</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003390</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037294312.1"/>
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    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="370" mass="40793" checksum="58333FBADF9E779C" modified="2022-01-19" version="1">MPNNIQIILDGKTATTSGSSNERATKADSTSPNTPSHNSAESASSNQSSSNKDNSSTDHSHGFFNFLLQACVISILVICIYEKGRKRGAQDLTAQLPDGKIFTDREGLRKEEEELKQTLETFMVFQPCGKCDGAFNVHHDILRNKRKELAEKVEGLDKKELEHVRQRAMVLEGKLKRQKASIEMDRARESPREAVTAGILPDETYTETVDGLYYETPSCSGEKADPPMEQAEMFPSANLVAQVPSQGPEMNETPRTVRHRENSMAHTKPDTRTTGPPRMKTGSSPASHVVLKSLSADYDAASEEMGQIKRAKEALESRERELEAQDKDRKERVQELTAPKSDQMSSIASGMLLGSALAFGVTMMMSVVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB45</accession>
    <name>A0A8H6CB45_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
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            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037294223.1"/>
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    <dbReference type="Proteomes" id="UP000593566">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:site of polarized growth"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ADF_drebrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_Abp1_fungi_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ADF-H"/>
    </dbReference>
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        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF25">
        <property type="entry name" value="DREBRIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="ADF-H" evidence="5">
        <location>
            <begin position="5"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
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            <end position="750"/>
        </location>
    </feature>
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        <location>
            <begin position="785"/>
            <end position="843"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
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        <location>
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            <end position="643"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="751"/>
            <end position="789"/>
        </location>
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        <location>
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            <end position="262"/>
        </location>
    </feature>
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        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
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            <end position="307"/>
        </location>
    </feature>
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        <location>
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            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="484"/>
            <end position="502"/>
        </location>
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        <location>
            <begin position="585"/>
            <end position="602"/>
        </location>
    </feature>
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        <location>
            <begin position="619"/>
            <end position="632"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="665"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="843" mass="88706" checksum="F3E3D3D4300FF919" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBG4</accession>
    <name>A0A8H6CBG4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149795.1">
        <property type="nucleotide sequence ID" value="XM_037294414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBG4"/>
    <dbReference type="GeneID" id="59331903"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051033">
        <property type="entry name" value="SH3BGR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232">
        <property type="entry name" value="SH3 DOMAIN-BINDING GLUTAMIC ACID-RICH-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232:SF0">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="408" mass="42927" checksum="C9FF0DF056B39C3F" modified="2022-01-19" version="1">MADQTPKSYHTDPNLYLYTSLTAGSSQIITATSRMETILKANKIRFQALDVATDEKARMLWGRRAGKRKLPGLVRMGMIVGDLEEVEEWNEYGELRDNVEPGPKPAPSAPATPSKAPTAPSLEILSKAPATNPVTPSKPPALVSSTETPPRAAPSSTDSPLTTAMRQAGFEAARKAGDAKSKARAEALQASKAQADTPTSETLQKLDNSSPTGMPPPSGATEAGEALKPETGNTSAEGNADDVPVTEEGKGEAEEEGKNSNVHGLGAMDADKVETDGKETKMLRSKKAEDSATEATVASESEEVLEGRGQAESFSEDIPNSKEVEEAANASIMHDKSHIVLVGSREADRTPPSEPVDEKEELSDPKEKDGSKLKEATNVSDEGQGLPGDKTQDQPAASGVEAGNSVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBL1</accession>
    <name>A0A8H6CBL1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149711.1">
        <property type="nucleotide sequence ID" value="XM_037294330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBL1"/>
    <dbReference type="GeneID" id="59331819"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="416" mass="48104" checksum="DB72EEC2E05D07CE" modified="2022-01-19" version="1">METSGATRFDMTALFHDLPTYREAPPVPRHSAMSRARKQYKRKNDLRYRMKGRKRRFAIFRVLPTEIIVLVMCHTRSGDLANLIQTDNSMNEIFQNHKMWLFKRMQICQFSEFSGWFGDMPGFDGPILGNNRTSEQIQCLKDVVFTFDWRITEVAPSDDEAAKVFLHLLERYGGWRYLYFLNAVKRHMEQEAQSLYKFSHMAIPSMNEESAKAMVLCFARMSWRAATVLGGKMKEPADMSARVEYRLKFFQREPRTLQKLMTKILRLLIYRIGKRLQVTDTVTLYQYINQSAGFGILIPVPSEEELYDSASAIMTRLLLECCFFYGITFAMQLCEEPVNEEVKAAQSWIIRRFEHALMYATRFSNVDTVLIADSSIREGSLWAAGIGFPTLGWFRSNKASAGGVMLAFEHDGMRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBM8</accession>
    <name>A0A8H6CBM8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_003490</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149793.1">
        <property type="nucleotide sequence ID" value="XM_037294412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBM8"/>
    <dbReference type="GeneID" id="59331901"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16273">
        <property type="entry name" value="SNM1A-1C-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12650:FF:000007">
        <property type="entry name" value="DNA cross-link repair 1A protein, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000038">
        <property type="entry name" value="DNA cross-link repair protein pso2/snm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF6">
        <property type="entry name" value="DNA CROSS-LINK REPAIR 1A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="7">
        <location>
            <begin position="729"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="104"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="349"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6220358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="890" mass="98539" checksum="08FEF4F5E9892458" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBP0</accession>
    <name>A0A8H6CBP0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149706.1">
        <property type="nucleotide sequence ID" value="XM_037294325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBP0"/>
    <dbReference type="GeneID" id="59331814"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="249" mass="27761" checksum="2C9F28795F1AFD92" modified="2022-01-19" version="1">MPIPHPSFLYRESTEPPTVALPLEARKIHFCHSALLGVNPYSSSHLYKRVGPWTILNPSGVGTYIPTGYAFRHDMVHGGRCARTVAENEVKCYVRTLVCKGGKLEGWWPRTENYRGDGHCFDCIFLRPNNFGCTIDAEPAADTPEPGTPSPEEPQPDIQIVGTWDPLPEPATAGSEADPRGSEAPETALAREESVEYDESQAGDTSSDDESDDVSPTAHVEREARRQREARMETRRMMLVEQMRRVHEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC56</accession>
    <name>A0A8H6CC56_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150172.1">
        <property type="nucleotide sequence ID" value="XM_037294094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC56"/>
    <dbReference type="GeneID" id="59331582"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="178" mass="20189" checksum="FCEF17A9B56B1219" modified="2022-01-19" version="1">MPKHQRPKAHCISEVVNLCSETRQDSSQNLPSSHRTFALQDVLLDDRIEPWGLRPVSRTSNTEVRRGSFNAGQVFVPGDLRSFRVLEKLKADTLPFIESSPEENTFKAVDEEFETARQMNVCKGNHHCAFAAKERKNLVDKYIHDAINTVGQVHRPEVSLPASLEQMTPLLTTTTFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD77</accession>
    <name>A0A8H6CD77_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MGS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_002775</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PurH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150529.1">
        <property type="nucleotide sequence ID" value="XM_037293700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD77"/>
    <dbReference type="GeneID" id="59331187"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01421">
        <property type="entry name" value="IMPCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.20:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024050">
        <property type="entry name" value="AICAR_Tfase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005492">
        <property type="entry name" value="PRK07106.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="600" mass="65747" checksum="FDB1EDB64C853C29" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD87</accession>
    <name>A0A8H6CD87_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PABS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002068</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150648.1">
        <property type="nucleotide sequence ID" value="XM_037292995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD87"/>
    <dbReference type="GeneID" id="59330482"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037959">
        <property type="entry name" value="MFS_SpdSyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="11"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="291" mass="32936" checksum="C2390D528B8054E5" modified="2022-01-19" version="1">MSEITHPTIKDGWFREISNMWPGQAMTLKVNQVLHHEKSKYQDVLIFESSDHGTVLVLDNVIQCTERDEFAYQEMITHLAMNSHPDPKSVLVIGGGDGGVLREVVKHSTVETAILCDIDESVIRLSKKYLPGMSIGFQHPNVSTHIGDGFKFLADKKNEFDVIITDSSDPEGPAESLFQKPYFELLYGALKEGGVITTQAENQWLHLSLITDLKKSCKEVFPTVEYAYTTIPTYPSGQIGFMVCSKDKDRDVTTPVRSWDTQEEDKLCRYYNKEIHKASFVLPTFARKALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDI9</accession>
    <name>A0A8H6CDI9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002281</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150860.1">
        <property type="nucleotide sequence ID" value="XM_037293207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDI9"/>
    <dbReference type="GeneID" id="59330694"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000721">
        <property type="term" value="F:(R,R)-butanediol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034079">
        <property type="term" value="P:butanediol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08233">
        <property type="entry name" value="butanediol_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF23">
        <property type="entry name" value="(R,R)-BUTANEDIOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="9"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="359" mass="38680" checksum="3245443EFE0357E6" modified="2022-01-19" version="1">MMKAVRFHGQRDIRLDDVEIVQCGKGQVKVKPAFAGICGSDLHEYLGGASLMPTSPHPITNEKVPLTLGHEFSGTIEEVGEGVHDIKVGSRVAVQPIIYDGTCNACKEGYINCCDNNGFVGLSGWGGGLSEHVVVPRKAVYEIPDSVSMDVAALIEPLSVAWHAVNTSPFKTGDSVLILGGGPIGLAVIQMLVVKGAEKIIVSEVASRRREFARQFGAHYVLDPTKDDIVSRVRDLCDGSGVNVAFDAAGVQAGLDQAVQAIRARGTLVNIAIWEKQATITPNWFCFRERKYMGVATYQDGDFQDVIDAIASGKLNPGPMITGKIRMDEVEEKGFKALVNDKDNHIKILIDIGLGVPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDR0</accession>
    <name>A0A8H6CDR0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nuclear distribution protein PAC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Lissencephaly-1 homolog</fullName>
            <shortName evidence="11">LIS-1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">nudF homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PAC1</name>
        <name evidence="11" type="synonym">LIS1</name>
        <name evidence="15" type="ORF">HO133_002369</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for nuclear migration during vegetative growth as well as development. Required for retrograde early endosome (EE) transport from the hyphal tip. Required for localization of dynein to the mitotic spindle poles. Recruits additional proteins to the dynein complex at SPBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Self-associates. Interacts with NDL1 and dynein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
            <location evidence="11">Spindle pole</location>
        </subcellularLocation>
        <text evidence="11">Localizes to the plus ends of microtubules at the hyphal tip and the mitotic spindle poles.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">Dimerization mediated by the LisH domain may be required to activate dynein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat LIS1/nudF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDR0"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051012">
        <property type="term" value="P:microtubule sliding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000342">
        <property type="entry name" value="Nuclear distribution protein PAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000002">
        <property type="entry name" value="Platelet-activating factor acetylhydrolase ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03141">
        <property type="entry name" value="lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017252">
        <property type="entry name" value="Dynein_regulator_LIS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037190">
        <property type="entry name" value="LIS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056795">
        <property type="entry name" value="PAC1-like_LisH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24951">
        <property type="entry name" value="LisH_PAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037647">
        <property type="entry name" value="Dynein_regulator_Lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109925">
        <property type="entry name" value="Lissencephaly-1 protein (Lis-1, PAF-AH alpha) N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 11" id="KW-0813">Transport</keyword>
    <keyword evidence="3 12" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PAC1-like LisH-like dimerisation" evidence="14">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="153"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="198"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="248"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="78"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF24951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6221513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="457" mass="49582" checksum="C817A9B4D3E475DF" modified="2022-01-19" version="1">MSQLLTTRQAEELHKSIIAYLSSTKLSSSAAALREELGIGDSFDDATSKKYEGLLEKKWTSVVRLQKKIMDLESRNSNLQTELDSATPTSLSRRNQDPASWLPRSPARHTLQSHRSPVNCVAFHPIFSSLASGSEDTTLKIWDWELGELERTLKGHTKPVLSCDFGGPRGGTLLASCSSDLTIKLWDPSDEYKNIRTLPGHDHSVSAVRFVPSGAAGSPASGNLLVSASRDKTLRIWDVSTGYCVKTLRGHVDWVRDVAPSFDGRWLLSAGNDQTARSWDASSGEAKATFLGHEHVIECCIFAPAASYPHLATLAGLKKPPPPSSSGEYLATGSRDKSIKIWDSRGTLIKTLIGHDNWVRALVFHPGGKYLLSVSDDKTIRCWDLAQEGKCVKTIDDAHGHFVSCLRWAPGVMKGPVVNGSNGDAGGKGKDDTGGQETIRCVMATGSVDLNVRVFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDR9</accession>
    <name>A0A8H6CDR9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001651</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037292579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDR9"/>
    <dbReference type="GeneID" id="59330065"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="124" mass="14174" checksum="9740F88D5A00493E" modified="2022-01-19" version="1">MINKNQCQTGIAYRPEMSLIRLAVRIGGSLGASSYRRELNKKRFQEDYFRYKVATISSTYIPTSKSQVIFTQKEGNGKEKLFIDEVLYNGSSWTYRLRRGDTKGEGVLKNSFAETDLSEKASVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDY1</accession>
    <name>A0A8H6CDY1_9LECA</name>
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            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="6" type="ORF">HO133_002251</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000014">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
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            <begin position="131"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="626"/>
        </location>
    </feature>
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            <begin position="664"/>
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        </location>
    </feature>
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    </feature>
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        </location>
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            <begin position="1114"/>
            <end position="1177"/>
        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="1236"/>
            <end position="1316"/>
        </location>
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        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="450"/>
            <end position="482"/>
        </location>
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        <location>
            <begin position="536"/>
            <end position="550"/>
        </location>
    </feature>
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            <end position="579"/>
        </location>
    </feature>
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            <end position="610"/>
        </location>
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            <end position="706"/>
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        <location>
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        <location>
            <begin position="1297"/>
            <end position="1308"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6221396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1735" mass="190547" checksum="9947706136FB36C0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE55</accession>
    <name>A0A8H6CE55_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="ORF">HO133_002430</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>Lecanoromycetidae</taxon>
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        </lineage>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037293356.1"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="Znf-RING_euk"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF6221574.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="293" mass="32403" checksum="FEFF5233EDE829A2" modified="2022-01-19" version="1">MAVHLKALLEKTQLIQPNEVENQICPVCYEDYLQPPSREFPRKLPCGHVIGTECLLLWASSQTHATSINCPWCTHPITHSIDTQYLRTVIDAYAEAALQHIAAQIERAIVAVDRALTHGCWSLLSLAVVFLSLGLYFDGFFDCLPLGFLYVCTGLAVKCCLTSYPRLGVALMVLGFYVGTFVDSDLGHVLLRYGKAPFHAAIARAYFDHTRLVLGLSVTLLVAQAAWGNPIGQVIAFKAELPGLPEMVVDGGSTAKDIRVMKRKSAEEKRKRMEGYELGWTGRKSVNSDMEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE89</accession>
    <name>A0A8H6CE89_9LECA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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            <taxon>OSLEUM clade</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037292881.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF4">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="287" mass="32950" checksum="90BDB96AB9F21CF2" modified="2022-01-19" version="1">MPSRQRGGLLQEAREVYNRDFDAQFPLSPSVHGSSGSSEAPSSEDTDEENDRQQENQERIGIQTWLRYRRDHPHACGHRPRPTPAQPFNFMGLPTETRCEILRLLLERIKEVTDVSLKKSFESYNMVPVDTRLFAVSRQMQVDAEVVFFRSNTIHVHVEHYDLPFFITNPPPSIRELRKVHLSISHYSSMPTQPVLAENLEKLADLLKDCKKLVQLKISQLCRSGEFPKVSTHVLRDSLEYFTRVRGVSEVVLTDLEPPKDDNAALIYAPEKVTTRLKAIMQSPKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEB9</accession>
    <name>A0A8H6CEB9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151278.1">
        <property type="nucleotide sequence ID" value="XM_037292739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEB9"/>
    <dbReference type="GeneID" id="59330225"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033987379" evidence="1">
        <location>
            <begin position="19"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="171" mass="18531" checksum="BAA7169E71478A8D" modified="2022-01-19" version="1">MYATTLLPLLALAITTLTAPLETRRWGPEEYYSLGCPTNTTKPYASQYQQTEVLNTFANEVFVNQEVALGYDTYAAVDFINHAPEVAGNGTAIVIPFQSAELKGGSVVLQRVFVGTNLTGTSFGTIHFRGNSVAFGEGDIAGIWRMIGTCLVEYWDVASGVEASENPIAYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEX9</accession>
    <name>A0A8H6CEX9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AA9 family lytic polysaccharide monooxygenase</fullName>
            <ecNumber evidence="1">1.14.99.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endo-beta-1,4-glucanase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycosyl hydrolase 61 family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001954</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Lytic polysaccharide monooxygenase (LMPO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and/or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1-&gt;4)-beta-D-glucosyl]n-1 + [(1-&gt;4)-beta-D-glucosyl]m + acceptor + H2O.</text>
            <dbReference type="EC" id="1.14.99.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM).</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.56" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151421.1">
        <property type="nucleotide sequence ID" value="XM_037292882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEX9"/>
    <dbReference type="GeneID" id="59330368"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182:SF2">
        <property type="entry name" value="LYTIC POLYSACCHARIDE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034777219" description="AA9 family lytic polysaccharide monooxygenase" evidence="3">
        <location>
            <begin position="23"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Auxiliary Activity family 9 catalytic" evidence="4">
        <location>
            <begin position="36"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="215"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="305"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="250"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="392" mass="38108" checksum="2282513B095E85A6" modified="2022-01-19" version="1">MYSINNILAISGLALFTTTAQGHMVMASPPPYGSPDNSPLNADGSNFPCKATSNSGGTVTNMAIGAPQTLSFLGESVHGGGSCQVSLTTDTPATKSSKWMVIHSIEGGCPAKNVAGNLGADNSASAPDPDTYSFAIPTGIAPGAYTLAWTWFNKVGNREMYMNCASVKVTGGSSKRDTPLNETDFAIPDLSGRETPSFPAMFVANIPTSDCLTADSKDVQFPTPGTSVEKDGVASDLAPPTGPNCGASGGSASSGASSSSSSGAQAASPADGSSAGASAAAPSAASPAAAAPSVAPAAAAAPAPSAASPAAASPSTDFTSGSSGSTGSSGSTCSSSGTMVCSADGKQFAICGASGTAPSFQPVADGTSCTNGAIAAAKSKRSSVKFAAGYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF28</accession>
    <name>A0A8H6CF28_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001476</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151825.1">
        <property type="nucleotide sequence ID" value="XM_037292406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF28"/>
    <dbReference type="GeneID" id="59329892"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12619">
        <property type="entry name" value="RRM2_PUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12622">
        <property type="entry name" value="RRM3_PUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000117">
        <property type="entry name" value="Nuclear and cytoplasmic polyadenylated RNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000224">
        <property type="entry name" value="Nuclear and cytoplasmic polyadenylated RNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000134">
        <property type="entry name" value="Nuclear and cytoplasmic polyadenylated rna-binding pub1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038">
        <property type="entry name" value="RIBONUCLEOPROTEIN RB97D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038:SF1">
        <property type="entry name" value="RIBONUCLEOPROTEIN RB97D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="84"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="184"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="310"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="379"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="392"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="474"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="518" mass="55446" checksum="5B9D7AE6288F39BE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF77</accession>
    <name>A0A8H6CF77_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151715.1">
        <property type="nucleotide sequence ID" value="XM_037292296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF77"/>
    <dbReference type="GeneID" id="59329782"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="14"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="466" mass="53492" checksum="BCC34E6AD5E3DA9D" modified="2022-01-19" version="1">MILKHHSPAESSSTCYIERLPPEVAELICYSIDEKDVPNLRLVSKFWNNIATRFLLGNVNLVFKPESFQRLLDISRHPLISKHITSLYYEPNTLDDYTTQYDWESNIINNSDTGAFGALPGPDASERELRAWRRNITKIRERPRHNYSRSYLEKAYKEYTRMYAEQEELRRRDYGLQEFSDAMSRLPNLSEICMNHGWAIYVRRKDAINAFAAGLHYAGGDYCGIPFMSSLLLAVHEAGIKLSILQLGSVDWKFLQQSDEVLERMKSTLHYLTTLELAISTGMDQSGNDIGVEIPKCRRYLSKNAKLAEFLAAAPNLKNLTISFDWYEPFCPAELNQTVGSTTWPCLESITWENIDATSENINCFFERHASTLKHVTMRTMRLLEGTWIETLQKMNKVLNLKSACIRGDLLGENPPQHWDLEPDLWAEHTDSRSQGNRTSKAIQDFLLQGGSCPLIDQDAHPQRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFK4</accession>
    <name>A0A8H6CFK4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152004.1">
        <property type="nucleotide sequence ID" value="XM_037291642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFK4"/>
    <dbReference type="GeneID" id="59329123"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790:SF4">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="1">
        <location>
            <begin position="46"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="384" mass="43194" checksum="A8749D760D31B5D3" modified="2022-01-19" version="1">MDRKSWYRDMWKLLEIINTESKSTEHIQIEECNPRTTTITQQNSLQSTLLCLPLEILEIIASYAIGANGNLIHIKQTRTDCVTLHALGKVHSRQRIPGFGKNNLGPVPLPGLRHAICNARKTEDAAYAEAISNAGWVHTPQYEIERNATRHLYCGCCGAYDPTDVTELEKLHLALFASCRQLYHLGNTTLYAKNTFSFDDALSFGQFLAGLDPKQQKMIRSLHLSRPSVDTPNRASDRSAWMSALEPSSIRALEGLRTVHLCLEMNIHTHNSWYAQTSYKVMVLESILASFLQLRVLPLTKATVIISDDVTAFNSTTGGSSLHGRWTVAEKNEWAEKTRLKLLEPYVEGTEVSLDSREEAKKGMTMTKRFKKVFQRAGGSKLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFL3</accession>
    <name>A0A8H6CFL3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">homocitrate synthase</fullName>
            <ecNumber evidence="5">2.3.3.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_000630</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>acetyl-CoA + 2-oxoglutarate + H2O = (2R)-homocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="2.3.3.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:12930"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-IPM synthase/homocitrate synthase family. Homocitrate synthase LYS20/LYS21 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.14" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151930.1">
        <property type="nucleotide sequence ID" value="XM_037291568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFL3"/>
    <dbReference type="GeneID" id="59329048"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00028"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004410">
        <property type="term" value="F:homocitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07948">
        <property type="entry name" value="DRE_TIM_HCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000002">
        <property type="entry name" value="Homocitrate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000032">
        <property type="entry name" value="Homocitrate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02222">
        <property type="entry name" value="Homocitr_synth_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048253">
        <property type="entry name" value="DRE_TIM_HCS_fun_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011872">
        <property type="entry name" value="Homocitrate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02146">
        <property type="entry name" value="LysS_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF48">
        <property type="entry name" value="HOMOCITRATE SYNTHASE, CYTOSOLIC ISOZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="14">
        <location>
            <begin position="57"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6222584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="499" mass="54498" checksum="DC69BD56F60FA5E8" modified="2022-01-19" version="1">MCPTCNGDVVNGTNGDVPATTNGHVNGTNGYGQHTPHNPRRQPFASVGDYLSNLSNFKIIESTLREGEQFANAFFDTDTKVKIAKALDAFGVEYIELTSPASSEQSRKDCEIICNLGLKAKILTHVRCAMDDARIAVETGVDGLDIVIGTSPQLMQHSHGKSMDYIRDTALEVIQFVKSKGLECRFSSEDSFRSDLVDLLNIYSIVAKAGVDRVGIADTIGCASPRQVYDLVRTLRGVVQCDIETHFHNDTGCAIANAYCALEAGATHVDTSVLGIGERNGITPLGGFVARMLVADRDYVRSKYDLTKLKAIEDLVAAAVEINVPFNNYITGFCAFTHKAGIHAKAILANPTTYEIINPADFGMTRYVHFTSRLTGWNAIKSRVNQLGLDMTDSQIKSCTARIKAMADVRHLAIEDTDAIINAFYENLYSEHNNEKPLLDDMTVDEKIQFAQKEAELSEEPESQHLDQIVDQQTNVLAKNDVNGHEGVNVLNGETAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG83</accession>
    <name>A0A8H6CG83_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000854</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat MET30/SCONB/SCON-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152151.1">
        <property type="nucleotide sequence ID" value="XM_037291789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG83"/>
    <dbReference type="GeneID" id="59329272"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF745">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="272"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="375"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="461"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="502"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="542"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="582"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="624"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="710" mass="78094" checksum="CDEFE86A3DC73976" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGD7</accession>
    <name>A0A8H6CGD7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000753</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152052.1">
        <property type="nucleotide sequence ID" value="XM_037291690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGD7"/>
    <dbReference type="GeneID" id="59329171"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000038">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000031">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF337">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="36"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="387"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="548"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="659"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="572"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="624"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="661"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="726" mass="80768" checksum="DB4C69D30F2B576A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH35</accession>
    <name>A0A8H6CH35_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_000125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152500.1">
        <property type="nucleotide sequence ID" value="XM_037291065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH35"/>
    <dbReference type="GeneID" id="59328544"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF16">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034426254" description="Nephrocystin 3-like N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="3">
        <location>
            <begin position="196"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="429" mass="48621" checksum="7B076ECEE376CDE3" modified="2022-01-19" version="1">MDPLSISASIIALLQLSSTIIGYLSDVKGGPKELQKIRLEICSVLPMLSILQDQAEQAKAGGLWSSTLLSLDGPNGPIQQFREALEQLRLRLVPVEGWKKVGKAFTWPFEKDEIQRILSTVERRKLLFTLARQNDHIALSKAIKGDVRDVHERVNEIGEGVAQSQLSEKHHKIRLWLSGLDPSSNYNRALRKRLQGTGSWLIKNDVFRGWKQDPGPGSIIWLYGIPGCGKTILSSTILEHVLDSYPPTSNTAVLYFFFDFNDVNKQQHEAMIRSLLSQLSMHCVSVPPVLDTLYSSCMNGGRRPTFEALLETFHQMAIIFTTTFIILDALDECEERPELMADIEQLVGWKDTNLRILATSRREKDIEDSILPLTKDESRICMQSALVNADIRAYVHDQLRTNTKLQRWQKEPKVQTEIEDTLMKKVDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI09</accession>
    <name>A0A8H6CI09_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000404</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152778.1">
        <property type="nucleotide sequence ID" value="XM_037291343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI09"/>
    <dbReference type="GeneID" id="59328823"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034135958" evidence="2">
        <location>
            <begin position="28"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="723" mass="75519" checksum="CF19207A63C7563B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIE4</accession>
    <name>A0A8H6CIE4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152928.1">
        <property type="nucleotide sequence ID" value="XM_037291493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIE4"/>
    <dbReference type="GeneID" id="59328973"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="336"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="602"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="825" mass="91524" checksum="2FC92357731D0E5C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIU1</accession>
    <name>A0A8H6CIU1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010962</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SPT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153445.1">
        <property type="nucleotide sequence ID" value="XM_037301811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIU1"/>
    <dbReference type="GeneID" id="59339352"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22926">
        <property type="entry name" value="HFD_SPT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000023">
        <property type="entry name" value="transcription initiation protein SPT3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF16">
        <property type="entry name" value="TRANSCRIPTION INITIATION PROTEIN SPT3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="249"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="324" mass="36507" checksum="E653E181C0B2400E" modified="2022-01-19" version="1">MAEKLGAKYRQEIQQMMFVAGETGEPSSETTTLIEQIVHEQVIEMLKRSTALATRRGSRAITTADLFFLIRHDKAKTSRLKTFLSWKDVRKSARESDDKGGDAADAAVGDDALGGVDVAGGPSVPVSELSKRSKKKVKLPWDISSFFTEQLPEGEDEDDEEEEEANAATIVRLQAADRRTKGMTRDEYVHYSECRQASFTYKKAKRFREWAGFGIVTDSKPNDDIVDILGFLLYDIVQTLTEEALKVKQEEDLNKSRNGDSDAQSKKRKRETGLFDPPEEGTTPVTPKHVREAFRRLQEKPIPGRVRVWMGGPRALQRTELKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIW4</accession>
    <name>A0A8H6CIW4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010904</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153387.1">
        <property type="nucleotide sequence ID" value="XM_037301753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIW4"/>
    <dbReference type="GeneID" id="59339294"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000003">
        <property type="entry name" value="GTPase activating protein (Evi5)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000027">
        <property type="entry name" value="GTPase activating protein (Evi5)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000001">
        <property type="entry name" value="TBC1 domain family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="5">
        <location>
            <begin position="260"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="115"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="549"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="791"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="645"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="888"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="549"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="579"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="623"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="791"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="986" mass="107577" checksum="28509C6755E8F305" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ32</accession>
    <name>A0A8H6CJ32_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_011009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153492.1">
        <property type="nucleotide sequence ID" value="XM_037301858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ32"/>
    <dbReference type="GeneID" id="59339399"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="203" mass="23087" checksum="ED504AC8E2AAF0B1" modified="2022-01-19" version="1">MSTGGNNFSSSTYFTSELIILYCIEMLQTIVQYSALVFPHTFGRLAYWHLLLFYDNTGLLDLQYMIFATLPSCIYFIHYFAAARDFRDDDDDERLPEDRPGPFRVILGCLKYWFLVYKAFDLLAALDVVLRASGFCGKAILAIDLLMESTLVLLAKTIFFLFVSISVGAALEGFPFVQSVILDLQEAFFGRNEVPADEAFHEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ99</accession>
    <name>A0A8H6CJ99_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Extracelular serine carboxypeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_010750</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153236.1">
        <property type="nucleotide sequence ID" value="XM_037301602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ99"/>
    <dbReference type="GeneID" id="59339140"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF117">
        <property type="entry name" value="SERINE PROTEASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6224176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="57015" checksum="D7E559BB5655877D" modified="2022-01-19" version="1">MELSLERRQDASQYPAHTIDMPIDHFPNNPRYAPHTNATFSQRYYFDSTYYKPGGPVYLYIGGETSGPSRFSNLETGIIQILMNATNGLGVILENRYYGESFPYNTSTTDQLVYLTTEQTIADNAYFATHATFPGIKGNISAPISPWVLYGGSLAGAQTAFSVKQYGGLLYGGIASSAVIHAVLAYPEWYAPIQKFAPQDCVTSINNIVDKFDALVAANNTEGIKEFKAIFGLEALVDNRDFAMTIAFPIGGPMNYPTNTWQELNWNSTYGSQDFFHFCSNVTNLDAPENITAVDTVLSKYSNGEAWSNLGNYAQYVKQTLLPLCDGVPIDSTSCFGTQNQTFYADTTNSAARSYLYSTCTEQGAYQVAPATGPSLISRVLQVNYTQQWCEWAFPKGKFNSIPSTPNLNYYNHYGGFNFSADRLAFIDGNIDVWLDVCYHSNDAPARYSTDLHPEYLIAGAGHHWDSYGILDLDAEPQFIEQAHLWEIRTVRKWLRSFKSWKSNVRHSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJA8</accession>
    <name>A0A8H6CJA8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153306.1">
        <property type="nucleotide sequence ID" value="XM_037301672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJA8"/>
    <dbReference type="GeneID" id="59339211"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="277" mass="30644" checksum="160B06923BAFD31E" modified="2022-01-19" version="1">MTVHEKKVQPERNQPARTPLPVYSMLPSVLQSSLQPLVLVRRSLTDYGLRSRRRVTSLDTYRSESEHTQVVPDKMAVAGECTADSAEVPGISWKFARHGSNTLQTSLAESQTSDAQSQAFSRQLYLDAMAYLIQGLPSDLSDQETLHLKNALPDSLKDSTPPETTQSRHRNPSLLHRSLASTIIAICLLLRLALPYIKLFIAAASSYDRTHHVRERVFAFSVTAADLFGRRSMALASTAMTNEVVWGAVKYWVDGIRGGLNEGLGEGLKVIEAQNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJT0</accession>
    <name>A0A8H6CJT0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7907 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153594.1">
        <property type="nucleotide sequence ID" value="XM_037300777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJT0"/>
    <dbReference type="GeneID" id="59338313"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057229">
        <property type="entry name" value="DUF7907"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25484">
        <property type="entry name" value="DUF7907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7907" evidence="1">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="144" mass="15527" checksum="D3439A739A803724" modified="2022-01-19" version="1">MIAAFPTGKGTNVAILTNDTSKALTGQFNNTYWTFSGFSKTHSLYMEPTADSKNFPYYGNVGINSGFATPGFSEGDEDGLLVWNNTDMANGGSWFAFCPTLVPGHESAGLQDRVFWQSNQAPAEGMTNCTTIVFHGAFEGAQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJU0</accession>
    <name>A0A8H6CJU0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153731.1">
        <property type="nucleotide sequence ID" value="XM_037300914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJU0"/>
    <dbReference type="GeneID" id="59338450"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053029">
        <property type="entry name" value="RNA_pol_I-specific_init_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007224">
        <property type="entry name" value="TIF_Rrn11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28244">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28244:SF1">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04090">
        <property type="entry name" value="Rrn11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="450" mass="49476" checksum="F79EB5FBB4B60B3B" modified="2022-01-19" version="1">MSSILYFSPAFKPSLSLSDLPFSPRELSKKRKRDLEPLDGDGGDGGDEGLEADSAANGTSLPSGYSNASVTISSAALDVDTSPSDKTLHSQPSSHTDRQGLHASSFPQSFPHHIAGTAPSMSKGRISDELATLKPPLYVTTGSVPTAAAERVPSSTGSRQHHLNTITAILHRCLSEGDYIRASRAWAMLLRAEQNGRSMDLRTHDRWGVGAEILMHRESQMTQKTLDHNVIDFSSSTSEFSVKPENFEKAKEYYERVVLQYPYRKAFPNAIGSLNFSIAMFSLWIYTIKERSSIALMAVGGSDQGMDQTDAEANDDVQRSSASDMEPDRYRMREQVRRDTLQSAHEIATRLDGLLVSPPYSDSARFWKLCVGMYLWIANLSVATISSNYGSSSNGDDENLTVGSSSRLRNVSWSTPSDEEHRAGQARQKALARAEEAFQRVKIYGESSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKB1</accession>
    <name>A0A8H6CKB1_9LECA</name>
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        <recommendedName>
            <fullName evidence="10">PHD and RING finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010162</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
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            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153834.1">
        <property type="nucleotide sequence ID" value="XM_037301017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKB1"/>
    <dbReference type="GeneID" id="59338554"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15545">
        <property type="entry name" value="PHD_BAZ2A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047157">
        <property type="entry name" value="PHRF1/Atg35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618:SF20">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="5"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="130"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="186"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="319"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="420"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="67614" checksum="24E92AD72E186316" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKN7</accession>
    <name>A0A8H6CKN7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010296</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153967.1">
        <property type="nucleotide sequence ID" value="XM_037301150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKN7"/>
    <dbReference type="GeneID" id="59338687"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000640">
        <property type="entry name" value="Serine/threonine-protein kinase PRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="343"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="132"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="292"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6225100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="667" mass="73015" checksum="CDDC7D78AA0E926C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKP8</accession>
    <name>A0A8H6CKP8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">dihydroxy-acid dehydratase</fullName>
            <ecNumber evidence="14">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_010311</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153982.1">
        <property type="nucleotide sequence ID" value="XM_037301165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKP8"/>
    <dbReference type="GeneID" id="59338702"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050165">
        <property type="entry name" value="DHAD_IlvD/Edd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000:SF5">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24877">
        <property type="entry name" value="ILV_EDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="IlvD/EDD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase N-terminal" evidence="18">
        <location>
            <begin position="75"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase C-terminal" evidence="19">
        <location>
            <begin position="408"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF24877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6225115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="672" mass="71525" checksum="2393CB18EF830DB5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMK5</accession>
    <name>A0A8H6CMK5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154796.1">
        <property type="nucleotide sequence ID" value="XM_037299970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMK5"/>
    <dbReference type="GeneID" id="59337504"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="28143" checksum="FEA41250DE3272DA" modified="2022-01-19" version="1">MSPSAPITKLPAELLQAIADNLDIEEYMVLRRSYPQVWQSLKPPALSNLLKLEFSAFAIEHKVLACHICCRLRPLCKFADDIVHRGMLKRSRPERFCIDCGIDSGFEGYGSDDWVATLDGEMHARCPLCRDVGSVGRHYSPSTRCELTGSSFGELVDEVAYALQSMSLLNTRYFDGDPPLTRWLWTISDDARIQFQHTLMKAVFGEQAAASFGPALEPPYVLPDDELMTEFDTQMETADVRDWLKNEGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR85</accession>
    <name>A0A8H6CR85_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Karyogamy protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_007939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156143.1">
        <property type="nucleotide sequence ID" value="XM_037298807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR85"/>
    <dbReference type="GeneID" id="59336336"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051293">
        <property type="term" value="P:establishment of spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013889">
        <property type="entry name" value="Karyogamy_KAR9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37271">
        <property type="entry name" value="KARYOGAMY PROTEIN KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37271:SF1">
        <property type="entry name" value="KARYOGAMY PROTEIN KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08580">
        <property type="entry name" value="KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="567"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="732"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="769"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="573"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="609"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="648"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="776"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="798"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="832"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="877"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="919" mass="99477" checksum="E585CA9334249060" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRC9</accession>
    <name>A0A8H6CRC9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-lactamase/transpeptidase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007995</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156199.1">
        <property type="nucleotide sequence ID" value="XM_037298863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRC9"/>
    <dbReference type="GeneID" id="59336392"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050491">
        <property type="entry name" value="AmpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021860">
        <property type="entry name" value="Peptidase_S12_Pab87-rel_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46825:SF14">
        <property type="entry name" value="BETA-LACTAMASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46825">
        <property type="entry name" value="D-ALANYL-D-ALANINE-CARBOXYPEPTIDASE/ENDOPEPTIDASE AMPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11954">
        <property type="entry name" value="DUF3471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="3">
        <location>
            <begin position="32"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S12 Pab87-related C-terminal" evidence="4">
        <location>
            <begin position="429"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="534"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="547"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6228265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="61140" checksum="B03A72E5195101EB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS67</accession>
    <name>A0A8H6CS67_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156557.1">
        <property type="nucleotide sequence ID" value="XM_037297922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS67"/>
    <dbReference type="GeneID" id="59335427"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="89" mass="10955" checksum="64C2EB01FBE3B7C4" modified="2022-01-19" version="1">MYWTKCVIRTTRQYRRVARYSGNDHLSFDSHLFYERLAIIKQYFLRRTMDEAQKVKNAKGARYKIKKMYYHPYALRTYAVRLRALLKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7L3</accession>
    <name>A0A8H6F7L3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147118.1">
        <property type="nucleotide sequence ID" value="XM_037297682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7L3"/>
    <dbReference type="GeneID" id="59335186"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="98" mass="11011" checksum="7443793C5D1D3D20" modified="2022-01-19" version="1">MSLHTSLTVNSVGTWTCYESIEEFTHNAIDSRAWEFGTPYSDRHSEDCALYRADNTTVEDGQSMSDEGVPAVYGPFKMHDDEPSFLIGFETKDGSEMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7T1</accession>
    <name>A0A8H6F7T1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Malate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147872.1">
        <property type="nucleotide sequence ID" value="XM_037296691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7T1"/>
    <dbReference type="GeneID" id="59334191"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021851">
        <property type="entry name" value="DUF3455"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35567:SF1">
        <property type="entry name" value="CONSERVED FUNGAL PROTEIN (AFU_ORTHOLOGUE AFUA_1G14230)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35567">
        <property type="entry name" value="MALATE DEHYDROGENASE (AFU_ORTHOLOGUE AFUA_2G13800)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11937">
        <property type="entry name" value="DUF3455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034338827" description="Malate dehydrogenase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="24753" checksum="7B9DFB81362C9DA1" modified="2022-01-19" version="1">MVASCLRCLASSIVFFLALALTIEARPSKRSVLPNIPRNWPSGGNGLSGYNVPLSDGTQSLPAPNSTLKFITIGRGTQNYTCTSTSPSPVLVGAKAVLLDASPLLPLLPPGEGTAVLDLLPRYVIDFDFAAIEASQIPKLGIHYFDSNGVPTFDLGNNGLLKGKGLADIPAPANASVGSFDQGYGAVDWKTLTDAGGSVGLKEVYRVETAGGKAPPSCAGATKPICIDYSALYWFYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F854</accession>
    <name>A0A8H6F854_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006230</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147703.1">
        <property type="nucleotide sequence ID" value="XM_037297129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F854"/>
    <dbReference type="GeneID" id="59334631"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="5"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="300" mass="33246" checksum="C0227FE784CF287E" modified="2022-01-19" version="1">MSKLGLWYDDWLIIAASCPATKSPLRVPSDGYRRIGINIYETVVTVLRLKPDPGDYARSLRAVSDVLKGTFISEICWTIAICIVKCSILTFYWRLFGLQGGAIRIAIWAFFVVVVCWGIAVGVATIFQCSPIQYMWDPTIPGRCITDTYWFFVGSSIPHIITDLALIGLPMPRIWKLQMPRSQKIMVSAILGLGGLSNGEKATNEVSLLIWTGVEVNMSIVCACLPCLRPIITLIFGKLKSLREHKSTHERGSDLPMDMLKLDSPAAMLAVSRQQIWGEGERPMLDSLAPSKMEAATPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F897</accession>
    <name>A0A8H6F897_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-binding protein REB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006012</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148096.1">
        <property type="nucleotide sequence ID" value="XM_037296915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F897"/>
    <dbReference type="GeneID" id="59334417"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051651">
        <property type="entry name" value="DMTF1_DNA-bind_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380:SF2">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="509"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="514"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="88"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="148"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="242"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="372"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="777"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="400"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="784"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="809"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="908" mass="102671" checksum="6F60DE5609563181" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8B3</accession>
    <name>A0A8H6F8B3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148350.1">
        <property type="nucleotide sequence ID" value="XM_037296368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8B3"/>
    <dbReference type="GeneID" id="59333864"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034093108" evidence="2">
        <location>
            <begin position="21"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="151" mass="15327" checksum="D927F3F234185167" modified="2022-01-19" version="1">MHLSSLAIFVAVLAPMAVLASPQWHPHGPHGPPGKWHQRPPGPEPSATASIPAGILPPGGFLPTGVSGAGSTGIPKKLDVKKKKVGVSGVAGAGSTGTPKKVAENSAVVPAPLASASVGVTSYDKNIPTVTTALFNYLPRLSTIHFDRHIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8G4</accession>
    <name>A0A8H6F8G4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lysine-specific metallo-endopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147944.1">
        <property type="nucleotide sequence ID" value="XM_037296763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8G4"/>
    <dbReference type="GeneID" id="59334263"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034643851" description="Lysine-specific metallo-endopeptidase domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="33081" checksum="7B7830B6BA446B00" modified="2022-01-19" version="1">MHIRQVFPNAITTIFRLTLCVAIARCQTAGNEIGDYAESDTYLIVYCNANTTGSHAAYLQNLIPYMQSNLQAVLRDLDRGTASPAYRAFFKTTKNLDAVRQVFTDIINGPDVLTVGALNVTRWSPPTLVCADADEPLLQHLRDNCNTPTRPPMNTMQPAKIVSICPIFWTLPRVARKAACPWVVGGRLLTEPWNLRTTQYAVLVHELVHVYNRFDDRDEVYDLGDVVGLNATGSLGNAQNFASYAAAVQAGCTEFPAPLDSGGADDDGPHFLSAVIEVVKYVAMESGLFLLWLARARSLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8I1</accession>
    <name>A0A8H6F8I1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148108.1">
        <property type="nucleotide sequence ID" value="XM_037296927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8I1"/>
    <dbReference type="GeneID" id="59334429"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34776">
        <property type="entry name" value="F17F16.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34776:SF1">
        <property type="entry name" value="F17F16.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="55913" checksum="0728EA320A85A9A2" modified="2022-01-19" version="1">MDFGKLPQVLSSDRSNWKFFRSIIALFPFSVVDTRGLLSYIRNSATPFTRSIALTQTRFAQPNFTFNMAGTRRSTRQAATKPNPSEASSKSKPAAAAGTKRKAESEAPAKGKRGKKGAKKEQTTIEASMPSDDKNGDSKEDVEMKDEQEAEPAEASNGKTGADEVAQREEEVRDQEPMDEEKKEEPSAEKKEPETNGDAKENSEEEPKKNGFDALMDHTDKDEDKNVTEEGTGSKVSKAEDAVEDSSKREEATPSSILEKGIIYFFFRGRVGINEPTDVNDIARSYIVLRPVPHGAKLGDGPIGDAGNNRMLALPKKVLPVSPKDRFMTFVEKANISMDDIKSQLSSSDYATKTAGARHTPAATPIGEGVYAITQTGRETHLAYILTIPSELGEIQQGVGLRQRGSYITSAKNPQSSGPANANLPQGAEYPKEILDEFHGRGWMPLQPKLLNYENTQFLLIGHNDDALEKAAKPQDGEEEKPEKETPLEEMETLEHEDEIRVEHLKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F972</accession>
    <name>A0A8H6F972_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Plasma membrane ATPase</fullName>
            <ecNumber evidence="10">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_005084</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148694.1">
        <property type="nucleotide sequence ID" value="XM_037295996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F972"/>
    <dbReference type="GeneID" id="59333490"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000008">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="397"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="760"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="787"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="823"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="889"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="932"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="12">
        <location>
            <begin position="99"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="993"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6219259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1014" mass="111653" checksum="8E91425F1C311E29" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9R4</accession>
    <name>A0A8H6F9R4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149368.1">
        <property type="nucleotide sequence ID" value="XM_037294680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9R4"/>
    <dbReference type="GeneID" id="59332169"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="146" mass="15892" checksum="3C527008BA959F59" modified="2022-01-19" version="1">MSNSASNSNDHKRSDFLPGLEDLGKLLKACSPNQGKKSDRSRIPDGKDCQICNIKDTCKTVVQASNELIEQGSSISEKETQHNKDQGMINPTRKPTQRQPKRRPKTDMLKTAEQANEGAPTKAAMNISTLGDAEGLIDDPDAELGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA58</accession>
    <name>A0A8H6FA58_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">2-hydroxyacyl-CoA lyase</fullName>
            <ecNumber evidence="12">4.1.2.63</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_003411</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 2-hydroxy-3-methyl fatty acyl-CoA = a 2-methyl-branched fatty aldehyde + formyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:25375"/>
            <dbReference type="ChEBI" id="CHEBI:49188"/>
            <dbReference type="ChEBI" id="CHEBI:57376"/>
            <dbReference type="ChEBI" id="CHEBI:58783"/>
            <dbReference type="EC" id="4.1.2.63"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:25376"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an (R)-2-hydroxy-long-chain-fatty acyl-CoA = a long-chain fatty aldehyde + formyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:67444"/>
            <dbReference type="ChEBI" id="CHEBI:17176"/>
            <dbReference type="ChEBI" id="CHEBI:57376"/>
            <dbReference type="ChEBI" id="CHEBI:170012"/>
            <dbReference type="EC" id="4.1.2.63"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:67445"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.63" evidence="12"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149714.1">
        <property type="nucleotide sequence ID" value="XM_037294333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA58"/>
    <dbReference type="GeneID" id="59331822"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106359">
        <property type="term" value="F:2-hydroxyacyl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02004">
        <property type="entry name" value="TPP_BZL_OCoD_HPCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000006">
        <property type="entry name" value="2-hydroxyacyl-CoA lyase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000071">
        <property type="entry name" value="2-hydroxyphytanoyl-CoA lyase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000054">
        <property type="entry name" value="Putative 2-hydroxyphytanoyl-CoA lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045025">
        <property type="entry name" value="HACL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710:SF2">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="18">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="16">
        <location>
            <begin position="196"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="17">
        <location>
            <begin position="395"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6220279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="594" mass="62736" checksum="562DC7B8A75D0CF8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAG3</accession>
    <name>A0A8H6FAG3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inhibitor I9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003131</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150133.1">
        <property type="nucleotide sequence ID" value="XM_037294055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAG3"/>
    <dbReference type="GeneID" id="59331543"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000005">
        <property type="entry name" value="Proteinase inhibitor I2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052471">
        <property type="entry name" value="PBI_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288:SF2">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inhibitor I9" evidence="3">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="71" mass="7925" checksum="46B8FFCFDBB042A2" modified="2022-01-19" version="1">MPTMFNITLKQGASPEQIDKAKESAKSQGGTIKHEFTLIKGFTVEFPNDTVHTLQTDDHIHVEQDGEARTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAJ2</accession>
    <name>A0A8H6FAJ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_002667</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150421.1">
        <property type="nucleotide sequence ID" value="XM_037293592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAJ2"/>
    <dbReference type="GeneID" id="59331079"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051704">
        <property type="entry name" value="FAD_aromatic-hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865:SF2">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="5"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="421" mass="47942" checksum="66930ED0D2071ED9" modified="2022-01-19" version="1">MPSERVLICGAGVAGSILAFWLAKHDFQVVVVERSKAEQKAGQGLEIEEPALKVVKLMGILDKLNEKKTREMGFDLVDQQARSYGIFEVSGISPTGTLELMRGDLTEILYKTADESANVTFQFETTIQSLRQTQDKVIVDLESRNNKTIKVEEFDFVVGADGVKSRTRQLVMGLPEKLKCFKSVGAFVAYFSIPKEDQDWPYSRLCHFTNRRILWMRPIGKDSKMTSVHLIHGHDDVPTLRRANAAGDRLKQKEAFAELYSGLGWETPRVIEQMMRAENFYSDELVQVKLQKWSQHRVVLLGDAAWAPTPFTGEGNQLAIIGAWVLAQELSRNRNPVAFETYEKRLRSYVDNAQSIPLGGYAPYIFNPQTTWGIWLLRTIVRLFSWMIRFISWTNLAGMLPDQQQNDFDLEIEEVEAKVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBQ1</accession>
    <name>A0A8H6FBQ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001956</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151423.1">
        <property type="nucleotide sequence ID" value="XM_037292884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBQ1"/>
    <dbReference type="GeneID" id="59330370"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051178">
        <property type="entry name" value="TfdA_dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779:SF2">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT XANTHINE DIOXYGENASE XAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779">
        <property type="entry name" value="DIOXYGENASE RV0097-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="27"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="376" mass="42107" checksum="F5BA1B83334D4B06" modified="2022-01-19" version="1">MPSSTMTLSPQQISIHPISVPSSDVDFGAVIENVDLENLTDNDFSIIRDTLYKNHVVVLKNQASSSPKAQYELTHRFDPSAIGYGHGKTLDAKRSILHPDLKTVPHQPEVQIIGNGLVPEYEGLSKIKLVHPHHKKFHKHPIPEEDDLTHTRFYRWHIDAALYDLNPPKVTSLLAVSVPKGRRQTLRYDDGTGDELDVPLGTTAFVSGYKMYDILSDADKEFVRTSRIEYAAHPYIWMSAAKSRSDGLGLVSDNLELPSSELPLIDLSKVQILPMVWKNPVTGQFALQMHPSAVRKIHLATGEVIEDLKRVREIVHKLQRPGISPKLVYAHDWEEGDMVLFNNQGVLHSVVGAFREDEVRLFRQCNMAASEGPVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC53</accession>
    <name>A0A8H6FC53_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Raptor N-terminal CASPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001064</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152358.1">
        <property type="nucleotide sequence ID" value="XM_037291996.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59329482"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000278">
        <property type="entry name" value="WD repeat-containing protein mip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029347">
        <property type="entry name" value="Raptor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14538">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01302">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Raptor N-terminal CASPase-like" evidence="6">
        <location>
            <begin position="182"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1299"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="98"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="916"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="974"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="917"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="974"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1008"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1435" mass="159963" checksum="8B9C34575C95DFD4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FC57</accession>
    <name>A0A8H6FC57_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152326.1">
        <property type="nucleotide sequence ID" value="XM_037291964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FC57"/>
    <dbReference type="GeneID" id="59329449"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414:SF1">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="101" mass="10710" checksum="796D5DF27B2DFA90" modified="2022-01-19" version="1">MSPRAPLAVVSLVLIAGALLLMLLVVIGGAVDKNPTNQFYFLQADTSAIPGAPATSRWTFWNTCGDTNGRNACPHVHPAYAFDPQKNFGTTKGVPASLIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCH7</accession>
    <name>A0A8H6FCH7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chitinase</fullName>
            <ecNumber evidence="3">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_000848</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the glycosyl hydrolase 18 family. Chitinase class III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152145.1">
        <property type="nucleotide sequence ID" value="XM_037291783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCH7"/>
    <dbReference type="GeneID" id="59329266"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF47">
        <property type="entry name" value="ENDOCHITINASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 17" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GH18" evidence="19">
        <location>
            <begin position="21"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="561"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="710"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="860"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="571"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="716"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="760"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="788"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="823"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="867"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="880"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="931"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="967"/>
            <end position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6222799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1020" mass="103540" checksum="E51847CC8E415297" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCL7</accession>
    <name>A0A8H6FCL7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Imidazoleglycerol-phosphate dehydratase</fullName>
            <ecNumber evidence="4">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_000257</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152632.1">
        <property type="nucleotide sequence ID" value="XM_037291197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCL7"/>
    <dbReference type="GeneID" id="59328676"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000005">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="238" mass="25739" checksum="BF017D65792B21D7" modified="2022-01-19" version="1">MSKPPPCRTALINRNTNETKIQVYLSLDGGPLDPLPDNADFSAESVAPNAPIPSQTTIHHASQLKGSQQIWIWTGIGFLDHMLHAWSKHAGWSLRVRCRGDLAIDDHHTTEDTLLALGTAFTTALGPRTGLARFGHCYAPLDEALSRAVLDISSRPFFVGNFDFEGPMIGQLNSQMIHHGLSSWAMAAGVTLHVDVIRGSNDHHRAESAFKALAGACRMACARIEGREGEVGSTKGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD07</accession>
    <name>A0A8H6FD07_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cardiolipin synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000195</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152570.1">
        <property type="nucleotide sequence ID" value="XM_037291135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD07"/>
    <dbReference type="GeneID" id="59328614"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043337">
        <property type="term" value="F:cardiolipin synthase (CMP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000017">
        <property type="entry name" value="Phosphatidyl synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF60">
        <property type="entry name" value="CARDIOLIPIN SYNTHASE (CMP-FORMING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6223353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="25295" checksum="AEEFEFF34480E582" modified="2022-01-19" version="1">MFHQAAFFSSHLLSANGLHRPSPLSILLPVHENIYTLPNILTFSRLVAAPVVGYLIVHDMHMPALGLFVYAGVTDLVDGWIARKWGLQTVVGTVIDPMADKALMTVVTVTLAMKGALPVPLAVLILGRDILLGLSALYYRYISLPLPRTFTRYWDFSLPSAEVRPTTISKINTFLQLGLIGATMSGLAMGGLGETAQTALEISWWVVGTTTLWSGGSYLWGKDIVRILSKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD14</accession>
    <name>A0A8H6FD14_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14 15">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="3 14 15">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_010576</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:23732"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.33"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:23733"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 15">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 15">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="3 14 15"/>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153062.1">
        <property type="nucleotide sequence ID" value="XM_037301428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD14"/>
    <dbReference type="GeneID" id="59338966"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14 15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11 15" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8 15" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="17">
        <location>
            <begin position="13"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Mvd1 C-terminal" evidence="16">
        <location>
            <begin position="198"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR015950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6224002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="391" mass="42071" checksum="2308543D804663BA" modified="2022-01-19" version="1">MADQKIYRASTTAPVNIAVIKYWGKRDPSLNLPTNSSLSVTLSQSDLRTHTTASCSSTFEGPDTLLLNNAPQSLSTARSLACLKSLRALRSELEDAESSLPKLSTYSLRLVSVNNFPTAAGLASSAAGFAALVRAIADLYELQQSPTELSLIARQGSGSACRSLLGGYVAWDHGIRDDGLDSHARLVAPKSHWPDMRALILVVSAAKKGVSSTTGMQTTVQTSSLFQTRTKEVVPKEMAEMEKAIKSKDFATFAKVTMRESNSFHATCLDTDPPIFYLNDVSRAAIKAVEEMNEKAGRTIAAYTFDAGPNAVIYYLEADEDAVLKTFLPILRQKEGWGKTDTKIDGPGPASPQVVEVLEEGVSRVILTSVGEGPISVPEHLIDDEGKTVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDV0</accession>
    <name>A0A8H6FDV0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153724.1">
        <property type="nucleotide sequence ID" value="XM_037300907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDV0"/>
    <dbReference type="GeneID" id="59338443"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045030">
        <property type="entry name" value="LYSM1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932:SF8">
        <property type="entry name" value="LD22649P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932">
        <property type="entry name" value="LYSM AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="578" mass="62592" checksum="A9A0586D28CC9580" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDW9</accession>
    <name>A0A8H6FDW9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010096</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">RNA-binding nucleolar protein required for pre-rRNA processing. Involved in production of 18S rRNA and assembly of small ribosomal subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153769.1">
        <property type="nucleotide sequence ID" value="XM_037300952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDW9"/>
    <dbReference type="GeneID" id="59338488"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000167">
        <property type="entry name" value="RNA binding protein Jsn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052645">
        <property type="entry name" value="Pumilio_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47093">
        <property type="entry name" value="PROTEIN JSN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47093:SF1">
        <property type="entry name" value="PROTEIN JSN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="460"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="PUM-HD" evidence="7">
        <location>
            <begin position="597"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="675"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="748"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="379"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="535"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="965"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="171"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="544"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="982"/>
            <end position="1007"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1154" mass="125127" checksum="6766DB8BB5D2B7B2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDX2</accession>
    <name>A0A8H6FDX2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010876</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions as a zinc transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153359.1">
        <property type="nucleotide sequence ID" value="XM_037301725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDX2"/>
    <dbReference type="GeneID" id="59339266"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904257">
        <property type="term" value="P:zinc ion import into Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000014">
        <property type="entry name" value="Cation efflux protein/ zinc transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045316">
        <property type="entry name" value="Msc2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="529"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="599"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="761"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="792"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="11">
        <location>
            <begin position="498"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="624"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="632"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="661"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="681"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="720"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="740"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="910" mass="99230" checksum="1F4FB15E0791A92B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEK3</accession>
    <name>A0A8H6FEK3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">GAR transformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_009607</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6R)-10-formyltetrahydrofolate = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6S)-5,6,7,8-tetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GART family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154316.1">
        <property type="nucleotide sequence ID" value="XM_037300468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEK3"/>
    <dbReference type="GeneID" id="59338002"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.170:FF:000009">
        <property type="entry name" value="Phosphoribosylglycinamide formyltransferase (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="10">
        <location>
            <begin position="8"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6225607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="218" mass="24320" checksum="C2E3C98A07EB00A2" modified="2022-01-19" version="1">MANQTPVRITVLISGNGTNLQALIDARDDALENCSIVRVISNRMKAYGLHRAKEASIPTHYHNLIPYRENFAENARTEYDKDLAAIVLADKPDLVVCAGWMSILADTFLDPLAEAHVPIINLHPALPGQFNGAKAIERAHKAWLEGKIEKTGIMIHYVESEVDMGKPILVEEIPFNRGVDENIENLEQRIHGKEHKLIVLGTRMVAEEMQKRNSNGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEM6</accession>
    <name>A0A8H6FEM6_9LECA</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_009682</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154391.1">
        <property type="nucleotide sequence ID" value="XM_037300543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEM6"/>
    <dbReference type="GeneID" id="59338077"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="990"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="645"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="657"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="703"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6225682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1082" mass="120990" checksum="2310EAC5A51B7898" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FER3</accession>
    <name>A0A8H6FER3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154511.1">
        <property type="nucleotide sequence ID" value="XM_037300663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FER3"/>
    <dbReference type="GeneID" id="59338199"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="137"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="201" mass="22886" checksum="68C8A51DAF24D7AD" modified="2022-01-19" version="1">MAATTGWKDDLEVPHPFPVASNLPMNTPPLAESAVSSFSGSGGIRKSTIDMKKKTQLRISGPAPAHRIRAIYDQKIAALENEVNCLRNWWWVDMMDRPDKRDWGQDGRWSEIYKREHWLRGLELAANGWVKADFAREKMMQADMRAKEREARKAKEAAEKAKRELATAKIVLAAVRRELEEKNDQVAMDRQLAMTLLAATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFD7</accession>
    <name>A0A8H6FFD7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009176</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154863.1">
        <property type="nucleotide sequence ID" value="XM_037300037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFD7"/>
    <dbReference type="GeneID" id="59337571"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="330"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="99"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="398"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="412"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="529" mass="57121" checksum="805B94A4C857A68E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFI3</accession>
    <name>A0A8H6FFI3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein SSH4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009102</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the proteasome-dependent degradation of fructose-1,6-bisphosphatase.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154789.1">
        <property type="nucleotide sequence ID" value="XM_037299963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFI3"/>
    <dbReference type="GeneID" id="59337497"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12909">
        <property type="entry name" value="SPRY_RanBP9_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035782">
        <property type="entry name" value="SPRY_RanBP9/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="3">
        <location>
            <begin position="174"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="4">
        <location>
            <begin position="450"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="507"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="542"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="691" mass="75693" checksum="D1D8FE74C19FCB0A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFM9</accession>
    <name>A0A8H6FFM9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008964</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154652.1">
        <property type="nucleotide sequence ID" value="XM_037299826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFM9"/>
    <dbReference type="GeneID" id="59337359"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="10">
        <location>
            <begin position="74"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="199"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6226099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="718" mass="80757" checksum="032E7068AFE83FBC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHI0</accession>
    <name>A0A8H6FHI0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007732</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155938.1">
        <property type="nucleotide sequence ID" value="XM_037298602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHI0"/>
    <dbReference type="GeneID" id="59336129"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="80" mass="8862" checksum="96A45BF921303707" modified="2022-01-19" version="1">MAGEEIKKIGPAVGVLERVSSAHTKAFEYWIQKSVSEHMYRAKTSMFRNINQGAPGKAGDSRLVGIVKELRELANEMSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHT4</accession>
    <name>A0A8H6FHT4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156313.1">
        <property type="nucleotide sequence ID" value="XM_037298977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHT4"/>
    <dbReference type="GeneID" id="59336506"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034954659" evidence="2">
        <location>
            <begin position="19"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="246" mass="24773" checksum="A552DCF383A2F0FD" modified="2022-01-19" version="1">MQLTRLAWLGGFLNLAFGEPQGYGYGYGGFSFPSLKTTGTGTSPPTLSTGVSGTAPGTTVPSRSAPYGNYSSSSYFPSGTAPFSTGSSSTSADPNTTTISGSPTPSANATYGTVSGAPYKTSNSSISGYPTYYPSHGPYPLPSSLSRSTSYPSGTIGSAVSTKVSGNTTTIASTGDPSAKKTIASTGDPSAKTISASGTAGSTGLASAPSSFVTYTAPTGIPSEYYFHHKKPKRSAHYGGGYAYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI72</accession>
    <name>A0A8H6FI72_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156624.1">
        <property type="nucleotide sequence ID" value="XM_037297989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI72"/>
    <dbReference type="GeneID" id="59335496"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="143" mass="16709" checksum="8131BDA50ED8E3FB" modified="2022-01-19" version="1">MPKWTIEEEIVLLYYASRQVKYATIVDILAKKCHPNVRTVKQITHKAARLRKSCGQKEVVLRACWAASDREWDRKLADQWLFSRMEKPKLVELLEFDGETAAIIDEKNDLDNFVDIMFLHDGETADQSDQPEKHEARTARHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIB1</accession>
    <name>A0A8H6FIB1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dynein heavy chain, cytosolic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_007145</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156673.1">
        <property type="nucleotide sequence ID" value="XM_037298038.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59335545"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072384">
        <property type="term" value="P:organelle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000122">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000005">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000071">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000517">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000003">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000007">
        <property type="entry name" value="Dynein heavy chain, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000004">
        <property type="entry name" value="Dynein heavy chain, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000829">
        <property type="entry name" value="Dynein heavy chain, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532:SF4">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="1940"/>
            <end position="2078"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2232"/>
            <end position="2459"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2595"/>
            <end position="2749"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2937"/>
            <end position="3103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3199"/>
            <end position="3261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3425"/>
            <end position="3466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6229031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="4363" mass="494230" checksum="AA0D7C6245C774BF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIE7</accession>
    <name>A0A8H6FIE7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_007191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156719.1">
        <property type="nucleotide sequence ID" value="XM_037298084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIE7"/>
    <dbReference type="GeneID" id="59335591"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051414">
        <property type="entry name" value="Adenylate-forming_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439:SF2">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439">
        <property type="entry name" value="PHENYLACETATE-COENZYME A LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="3">
        <location>
            <begin position="536"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6229077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1047" mass="114692" checksum="E2606F661E3B5BDD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK71</accession>
    <name>A0A8H6FK71_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_004351</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157270.1">
        <property type="nucleotide sequence ID" value="XM_037295269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK71"/>
    <dbReference type="GeneID" id="59332759"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000001">
        <property type="entry name" value="ATP synthase gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039089">
        <property type="entry name" value="ATP_synthase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9 11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="4 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6230013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="298" mass="32361" checksum="A1EA9F29EE85962C" modified="2022-01-19" version="1">MLSRAVRPAFNAAAAVSARPIAADAAGYATLREIEGRLKSIRNIEKITKTMKIVASTKLNRAQRAMVDSRSYGETSNTVFEKAETKPIESEDKKTLIIVCSSDKGLCGGIHSGLSRATRRMLEKKPNSDLVVLGEKSKAQLGRSSGKNIQLSFAGVGKDVPTFADAQAIADQISLLPTDYADVQILYNKFINATSYEATPIEAFSEEAIRESPNFAAFEIDDEVLANLREYALANSLYWALAEGHACEQSARRNAMDNASKNAGDMINRFQILYNRTRQAVITGELVEIITGASASEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKA7</accession>
    <name>A0A8H6FKA7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004447</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157365.1">
        <property type="nucleotide sequence ID" value="XM_037295364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKA7"/>
    <dbReference type="GeneID" id="59332855"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="15"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="278" mass="30651" checksum="BA7F521D6349B111" modified="2022-01-19" version="1">MASHHTSSQASASASDRHASQSWPPENDELLMQARQRGLNWQPIASQFFPDKTANACRKRHERLMDKRNSADNWDGVKVETLAKAYVDVREQMWKILADRIGEKWQNVEAKCMEKGLKTLQTAGRTASRREARANLGHDFDSDEIIDSRFPPSRRERSRSHQDEEDGFNDSAIGPEDHPFVNGNAFGSSRDDHQPASYSAGGISNTSPSYSSARSFSINSNASSSLAGFTPTVTAAPQFPSIHQQPQTLPSFSAAFGMPSISAVLHHSPHHSPAVTTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKE0</accession>
    <name>A0A8H6FKE0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">mRNA export factor MEX67</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_004442</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the export of mRNA from the nucleus to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NXF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157360.1">
        <property type="nucleotide sequence ID" value="XM_037295359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKE0"/>
    <dbReference type="GeneID" id="59332850"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14342">
        <property type="entry name" value="UBA_TAP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000013">
        <property type="entry name" value="mRNA export factor mex67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000296">
        <property type="entry name" value="mRNA export factor MEX67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040736">
        <property type="entry name" value="Mex67_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057125">
        <property type="entry name" value="NXF1/2/3/5-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030217">
        <property type="entry name" value="NXF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005637">
        <property type="entry name" value="TAP_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10662">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10662:SF22">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24048">
        <property type="entry name" value="LRR_NXF1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22602">
        <property type="entry name" value="NXF_NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18444">
        <property type="entry name" value="RRM_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03943">
        <property type="entry name" value="TAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00804">
        <property type="entry name" value="TAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51281">
        <property type="entry name" value="TAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="12">
        <location>
            <begin position="420"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="TAP-C" evidence="13">
        <location>
            <begin position="642"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6230103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="695" mass="75022" checksum="6BE6D85315A77EB4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY29</accession>
    <name>A0A8H6KY29_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156586.1">
        <property type="nucleotide sequence ID" value="XM_037297951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY29"/>
    <dbReference type="GeneID" id="59335456"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="469" mass="52404" checksum="2EDB576349B186FB" modified="2022-01-19" version="1">MATSSTPSLDPPAATPSIGNAGPFMRAPREIRDNIYRFLLSTKHTKEFHGEPASASSAQSPNNPTEPGLIRYFHTSILFTNRQVYIEASNIFYMENLFIRINSVAANELYHPTNFGGYRSTGSSGRGLRVLSTGTKAQACTRHVMEINLIPSPLLFDHHGYHHFIFACDDLPVFCRALRSIDRFYRRELQPLQVMDLWFTIGDEVGTAGKGYLDSNASTDGNPVGYLTAATKIAAANCAGPDENRRATDESSASVYGEIKGSGSAMVNGIKDAKSRLLERPISIDSPRSRRLLEPFRAVYNVGYSYIDAPISERYRQEIHTSLSRARPSIHDQFLAVSSAFEEAIAVYDADDIALAIQKSKGTLDILSDFRYLSRDGDSTKSVPTGRYTGLMYREAMDKMHLIIYSNLARANLRFHSDLQQVRSAQDFVWQIRGIRARDAMWPEHPGSRRTMRISIPEARARATCVRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZH3</accession>
    <name>A0A8H6KZH3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004288</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157207.1">
        <property type="nucleotide sequence ID" value="XM_037295206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZH3"/>
    <dbReference type="GeneID" id="59332696"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000083">
        <property type="entry name" value="WGS project CABT00000000 data, contig 2.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054551">
        <property type="entry name" value="RSC4_Ig-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF19">
        <property type="entry name" value="PROTEIN POLYBROMO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22994">
        <property type="entry name" value="RSC4_Ig_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="253"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="170"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="354"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="411"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="433"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="467"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="504"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6229950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="678" mass="74388" checksum="5A0C15A4D22A1168" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZV8</accession>
    <name>A0A8H6KZV8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157524.1">
        <property type="nucleotide sequence ID" value="XM_037295523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZV8"/>
    <dbReference type="GeneID" id="59333014"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="141" mass="14874" checksum="E3948D205013A653" modified="2022-01-19" version="1">MSESTFHTTREDLRKAESQVSKKNNGSVPADSEVAQTMSIVDQNSKPKSEIIAERQSNLPLPDDPPVASDWNSSDASKVNVGSGGVESDVSYGGDIREPATSDSSARTDGETFGKNSSLPQGVGREGHDGLDGLPKDALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L030</accession>
    <name>A0A8H6L030_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157793.1">
        <property type="nucleotide sequence ID" value="XM_037295792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L030"/>
    <dbReference type="GeneID" id="59333286"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="452"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="539" mass="61497" checksum="56D61CE42B53BE91" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L085</accession>
    <name>A0A8H6L085_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000001">
        <property type="protein sequence ID" value="KAF6230745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157818.1">
        <property type="nucleotide sequence ID" value="XM_037290944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L085"/>
    <dbReference type="GeneID" id="59328423"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="274" mass="30887" checksum="22BF3D5B1113213B" modified="2022-01-19" version="1">MLPPLDTRKINALLRDGDEGEELECDPDDHIGMLKGMIAEDRNWSEGVLMLLELWPRTNPRFYRKKPMSTSHPLYRSGGPNNWWIELNNVYFGPLRLKPSSGGVINIYTFGEYAKSFQIMGCRQNWVAMISWIWEYANNLDAVKRKSEGSALQALVYYAFINAFAASNFSTRANDQRRRVEKPLLHQVLHPAKFKAQHGRLTKAKAKELAAAPKNPDSEMHDVDASSTASDMAISCGNTSLAIQTMGMRDDHRTPDATAVDIDFEALDVVDHDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6Z5</accession>
    <name>A0A8H6C6Z5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_006355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147378.1">
        <property type="nucleotide sequence ID" value="XM_037297253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6Z5"/>
    <dbReference type="GeneID" id="59334756"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000018">
        <property type="entry name" value="Heme/steroid binding domain protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF76">
        <property type="entry name" value="CALCUTTA CUP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="3">
        <location>
            <begin position="102"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6217943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="271" mass="30010" checksum="C8068B828F1CDD41" modified="2022-01-19" version="1">MTALRSRANLPPKDRHSPSAAPFTAEDGPSDSTSKPEPYLSDPEEGPSRLSLLDILRVLGGLFLLSSTLSYFITGDSILWGYRPAITRPARIKAWLRGPYDLTDAQLSIFNGTEPTLPILVAVNGTIYDVSASPHFYGPGGSYSFFAGRDATRSFVTGCFDTDLNGDLRGVEEMFMPLEGEAGDDPTKEGRRERKLRREREKRTARKKVEETVRGWERMFDGGKDGKYFKVGKVDRGPGSGWEGWGEVKELCQKAREGRPRRTAEEVGGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7A0</accession>
    <name>A0A8H6C7A0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_006356</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the GSE complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147379.1">
        <property type="nucleotide sequence ID" value="XM_037297254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7A0"/>
    <dbReference type="GeneID" id="59334757"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11385">
        <property type="entry name" value="RagC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000643">
        <property type="entry name" value="Small monomeric GTPase (Gtr2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039400">
        <property type="entry name" value="RagC/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF2">
        <property type="entry name" value="GH16429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6217944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="367" mass="41398" checksum="361F27437E5A8CEB" modified="2022-01-19" version="1">MSRTDGDAEHEPSKPDHNSKASSSSADHEETTAVNGLNGGLEPGNLLHVDHKPRLLLMGLKRSGKSSISNVVFRKMAPHETLFLETTTTIRKEAMHSFMDFQVWDLPGQIDYLDPEFDAESIFGGVGAMIWVLDAIDNYMEPVSRLTETILHLQHSYPDIKYSVFIHKVDSLTEDFRDDTMRDVRQRITDDLYDAGLENPPITYYGTSIYDHSIFEAFSKVMQGLVPQLPTFEALLNTVSASCRFEKAYLFDVYSKVYIASDTTPIDMKAYELCADLIDIIVDMSEVYGWDRGKDQQEIDPSKAVAAQSAESFVSNIKGYHLYLKEINRNLSFIGISNNSDFESGKVLVDQNMQAFQDTLLKVFPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7D9</accession>
    <name>A0A8H6C7D9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005674</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147762.1">
        <property type="nucleotide sequence ID" value="XM_037296581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7D9"/>
    <dbReference type="GeneID" id="59334079"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000196">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000012">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF201">
        <property type="entry name" value="MFS AFLATOXIN EFFLUX PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="500"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="43"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="532"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="539"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="577" mass="62104" checksum="4B291B91BBD78923" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8D0</accession>
    <name>A0A8H6C8D0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005984</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148068.1">
        <property type="nucleotide sequence ID" value="XM_037296887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8D0"/>
    <dbReference type="GeneID" id="59334389"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="29874" checksum="02B7CD4258DDA043" modified="2022-01-19" version="1">MASTMVMSGMEVTESVSNIKLSEAQREAEQTPSEPIQPAQPRGNYDVDESMRACELDCQNYAMSTWGKTAKVIVEVSSGEERTYFLKVVTMGAPGKHMCEGEFESLKTIHTPASPMKLAAGLANLHKESVSPTGKFGFHIATCHARVAQAVDTWEDSCKQTYIRQYKSRLPVSPPNDDWDARNILSSLTFNIANTIYIPGSYQRQVVYDDMTTLCKMFRAHYLKRDLKSVGSIDSENEQATAGMDADEEDGEEELEEEEEEEEEVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8N8</accession>
    <name>A0A8H6C8N8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IBR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005273</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148166.1">
        <property type="nucleotide sequence ID" value="XM_037296184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8N8"/>
    <dbReference type="GeneID" id="59333679"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="IBR" evidence="6">
        <location>
            <begin position="244"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="315"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="353" mass="38065" checksum="9FEC64A0F7038211" modified="2022-01-19" version="1">MEIEAPHSLELDDATATLILQLQSGDLEELLSVNKGKGRDGDVSDADLAVATYQQELQTRNTILADRCMGRSLTRAVISDAALLNESFAEEHTAARDRALAQSLAGINAPSAALEQSTAAYDLDDGFLARLTALYVSGWENEGDVLEDTVRSDSPVAAESSAWAASRQNPATTSYRHCTVCDLTKPLFDVCQTPCGHFYCQECIQTLFELSATDETLFPPRCCREKIPLQSVKIYLNSAVVKNFEEKSIEFETSDRTYCSRPACSSFIAGANISGEQAMCRECGTQTCTICKNNAHDGDCPEDLATQQVLETARENATPAGSAGRPVHATSGTKIGSWPERTKSSLAANLKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C934</accession>
    <name>A0A8H6C934_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148354.1">
        <property type="nucleotide sequence ID" value="XM_037296372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C934"/>
    <dbReference type="GeneID" id="59333868"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="263" mass="30002" checksum="2B6ABD3A18BA2705" modified="2022-01-19" version="1">MVISDYMAASRRSPLMRDQWRAGDDHRDSNRRRNRGTDDRRKISPAAPRETDVGLKIKGRAAADSAPGSPSDHKRSERDNTLGKDRARGSSRSPPRRRPRDEELVQRRESSGDRYPERPRHRDEGGQTDRRRITRSRSPQRELFGFREERRRHRSPIHSGRTDTFRPSSRRRERANSPPRSNRGDHYSSTYTDLSGSAGRFGDSVLGRETGLQDQGEFLLNVHVHVQSGAHLLVVGLKKRVNLLHPATVLYPALGIHQEGTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C947</accession>
    <name>A0A8H6C947_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005017</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148627.1">
        <property type="nucleotide sequence ID" value="XM_037295929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C947"/>
    <dbReference type="GeneID" id="59333423"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000257">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000153">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="17"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="335"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="337"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="368"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="402" mass="45034" checksum="FF88D31A384D203F" modified="2022-01-19" version="1">MAAQEGQAKPQIQPCRYKTGKTLGAGSYSVVKECVHIDTGRYYAAKVINKRMMAGREHMVRNEIAVLKRVSMGHQNILTLVDYFETMNNLYLVTDLALGGELFDRICRKGSYYESDAADLIRATLSAVAYLHDHGIVHRDLKPENLLFRTPEDNADLLIADFGLSRIMDEEAFHVLTTTCGTPGYMAPEIFKKTGHGKPVDVWAIGVITYFLLCGYTPFDRDSNLEEMQAILVADYSFTPLEYWRSVSLSAREFVKRCLTIDPQQRMTAHEALSHPFVDEMARQQRGEGARGGGDLLPVIKKNFNARRTLHAAIDTIRAINKLREGGAAGLMEGALSQQPKVKQQDNGNEAKWNEAKFTGQGGSQMEIDSRGNARGQTEEQIREQQRRVEETSRGLWGQARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9D8</accession>
    <name>A0A8H6C9D8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA primase</fullName>
            <ecNumber evidence="9">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005137</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148747.1">
        <property type="nucleotide sequence ID" value="XM_037296049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9D8"/>
    <dbReference type="GeneID" id="59333543"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.920.10:FF:000002">
        <property type="entry name" value="DNA primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
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            <end position="76"/>
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        </source>
    </evidence>
    <sequence length="514" mass="58688" checksum="84C21C1614EB64B7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
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            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
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        </lineage>
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        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
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    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
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            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        <location>
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            <end position="1647"/>
        </location>
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        <location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1759"/>
            <end position="1771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219584.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1799" mass="196116" checksum="4EB232CE371EEFD4" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA51</accession>
    <name>A0A8H6CA51_9LECA</name>
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        <recommendedName>
            <fullName evidence="7">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
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    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037294494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA51"/>
    <dbReference type="GeneID" id="59331983"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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    <dbReference type="PANTHER" id="PTHR24193:SF121">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24193">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
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        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="Pfam" id="PF13637">
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    </dbReference>
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        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="13"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="11"/>
    </dbReference>
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    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="432"/>
            <end position="464"/>
        </location>
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    <feature type="repeat" description="ANK" evidence="3">
        <location>
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        <location>
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            <begin position="759"/>
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        <location>
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        <location>
            <begin position="256"/>
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        <location>
            <begin position="308"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219747.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="876" mass="95176" checksum="781CA274A40C80C2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA93</accession>
    <name>A0A8H6CA93_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4440 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149227.1">
        <property type="nucleotide sequence ID" value="XM_037294539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CA93"/>
    <dbReference type="GeneID" id="59332028"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00531">
        <property type="entry name" value="NTF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027843">
        <property type="entry name" value="DUF4440"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011944">
        <property type="entry name" value="Steroid_delta5-4_isomerase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02246">
        <property type="entry name" value="SgcJ/EcaC family oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14534">
        <property type="entry name" value="DUF4440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4440" evidence="1">
        <location>
            <begin position="99"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="222" mass="24831" checksum="863FDBB5EFAA623D" modified="2022-01-19" version="1">MEEGQAGIAALSPESGIFIRGDLTPTQKENLDKNIAQETRTRFLQEGTTLIIQKLDVYSWTAASTYQKPHQYVPQTAVSKLNRQHPDRVRTVSKDIEQISAVLHAYQDALNNASVEDAVKLYTQDGIVMPQHSPSFVGIANVRKAYESFFEIIKFDVKFDIQEVMPVSPEYAFARTNSAGTTVVKGRGKGNEANQELFVFKKVGGEWKIARYCFSTTNPPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAC1</accession>
    <name>A0A8H6CAC1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Very long-chain fatty acid transport protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Very-long-chain acyl-CoA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_003990</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Acyl-CoA synthetase required for both the import of long chain fatty acids (LCFAs) (C14-C18) and the activation very long chain fatty acids (VLCFAs) (C20-C26) by esterification of the fatty acids into metabolically active CoA-thioesters for subsequent degradation or incorporation into phospholipids. The transport and fatty acyl-CoA synthetase activities are genetically separable and are thus independent activities. Esterifies VLCFAs in the peroxisome matrix. The VLCFAs are actively transported into peroxisomes by a PXA1-PXA2 heterodimeric transporter in the peroxisomal membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148956.1">
        <property type="nucleotide sequence ID" value="XM_037294909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAC1"/>
    <dbReference type="GeneID" id="59332399"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000019">
        <property type="entry name" value="Long-chain fatty acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF6">
        <property type="entry name" value="ACYL-COA SYNTHETASE FAMILY PROTEIN (CEFD1), PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="20">
        <location>
            <begin position="58"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6219521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="649" mass="72425" checksum="7B6BDF63949F9400" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAH1</accession>
    <name>A0A8H6CAH1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149066.1">
        <property type="nucleotide sequence ID" value="XM_037295019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAH1"/>
    <dbReference type="GeneID" id="59332509"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="702"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="262"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="805" mass="83227" checksum="795BD296DE5C3BBF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAP8</accession>
    <name>A0A8H6CAP8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-mRNA processing factor 4 (PRP4)-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003241</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149545.1">
        <property type="nucleotide sequence ID" value="XM_037294164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAP8"/>
    <dbReference type="GeneID" id="59331652"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.110:FF:000003">
        <property type="entry name" value="Pre-mRNA splicing factor, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000698">
        <property type="entry name" value="Putative pre-mRNA splicing factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.110">
        <property type="entry name" value="Pre-mRNA processing factor 4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036285">
        <property type="entry name" value="PRP4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158230">
        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="5">
        <location>
            <begin position="67"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="238"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="291"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="333"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="375"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="479"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="518" mass="56944" checksum="7026172D64419521" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAX9</accession>
    <name>A0A8H6CAX9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Scytalone dehydratase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003221</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the scytalone dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149525.1">
        <property type="nucleotide sequence ID" value="XM_037294144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAX9"/>
    <dbReference type="GeneID" id="59331632"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049884">
        <property type="entry name" value="Scytalone_dh"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02982">
        <property type="entry name" value="Scytalone_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scytalone dehydratase-like" evidence="4">
        <location>
            <begin position="188"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6220090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="339" mass="37909" checksum="E474C1E3BD3234C3" modified="2022-01-19" version="1">MSQREAPASLTPPSTQEQPMTDTPMPEAPFIEPASTTDHKPAVTADRADQTLQQQQDPAPEQAPPMTDMPLASTFPREHPPREPTRRQTIPRPYDRNTPPDRKPPHLSSYPTAPDSLPQTPTSGMSAPSAPRARTGTPVDRTPHLSSYPTAPGSASQTPQRRTSQTSQHGMSPAGWLVPPPANPALKISFEDWLSLSEITFEWGDSYDDKDWMRLRAILAPTLMVDYSQVNGHVWSSMPAEEFVRVMSNIGFVGDPLVHTQHHIGASKWSKISDDEAIGHHQLRASHQRYTALDLKTVENKGHGHAMVKHFYRKVDGQWKLAGLRPTVRWNEFEFEKYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB08</accession>
    <name>A0A8H6CB08_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149680.1">
        <property type="nucleotide sequence ID" value="XM_037294299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB08"/>
    <dbReference type="GeneID" id="59331788"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF8">
        <property type="entry name" value="BENZIL REDUCTASE ((S)-BENZOIN FORMING) IRC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="115" mass="12365" checksum="68D45AD66B226BF8" modified="2022-01-19" version="1">MATKNIIVNGASREEWERAFAVNVTGALRLIQAALPELRKNNGRIIFTSSGAAVAGYPTLGPYGATKSIPNHLALILSKEEPNVTTVAVRPGTVNTGLVRKLLDDERVVEDVRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBJ9</accession>
    <name>A0A8H6CBJ9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149922.1">
        <property type="nucleotide sequence ID" value="XM_037293844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBJ9"/>
    <dbReference type="GeneID" id="59331332"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="249" mass="27550" checksum="B69B91FB17363B37" modified="2022-01-19" version="1">MPGQRVDQMVSDMTDEEYRAAEENYENYHPQADLMSFEERMEHHKYEEELYEDQAQGRDSDVRARRADYASNPPGRSNYGGSRMSRSRGERLPCVIHAKTHRNTVATQASTMVAFGNHIAISVKHSVAGAGSVLVGAPGTTATNVLIQGRQGPSASDSERDELGLPTRKKRARNGRVFGKLTTAAMIAREMGKVNIIIVARQPHASRIQIRTMRAVPPTIMAIVVAPVEEGMNMRLPLKASHWSEILTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBU3</accession>
    <name>A0A8H6CBU3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150113.1">
        <property type="nucleotide sequence ID" value="XM_037294035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBU3"/>
    <dbReference type="GeneID" id="59331523"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="328" mass="37813" checksum="B940FE2986593A4F" modified="2022-01-19" version="1">MDPRLDLRGRPWRLDSEWALGMTVGAFMWMQEPPFVLDRARYAISIDHQQSRNVEQAVFVPDFEVATTNSQLEPGRKESYPLLPFRALEGICLPGAIQQRWEASIAASKSAEWRLWFKTLDQTQQKAHVQSLAKRNKDRFWAGLWLEYEEWSKPRHARCIAAATRALVIFERENVMRQMREAQKSVNVRRARMLEGYGFSAMEPEDLPEAEPLSAPLTPHDIVSLRLRLAPCYFRLPASTFRFATQIFSGKSYEEAVTEQKANFHSWTNESKVRRIEGHPDFVVWGNIAVHKDAVPKLTPKKKQPQTGKHRYQDKQSRSGCGEGCQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC29</accession>
    <name>A0A8H6CC29_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyltransferase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002942</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149944.1">
        <property type="nucleotide sequence ID" value="XM_037293866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC29"/>
    <dbReference type="GeneID" id="59331354"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050879">
        <property type="entry name" value="Acyltransferase_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23028">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23028:SF134">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08520)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Acyltransferase 3" evidence="3">
        <location>
            <begin position="69"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6220509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="494" mass="56601" checksum="C9C682FA8A9731B8" modified="2022-01-19" version="1">MKKTRKTESEAAPSSQKGLLAADNDPDTLWARPARTRYLKTIAFALLPSPVQRRLVPSGFKASRLHPTAYLDGLRGLASLSVCLLHYTSNFAPRLNASYGVSDDKMHSSPLQLPFIRVLYTGRPFVHVFFVISGFVLSLKPLRLARAHNYADLHTTLSSSVFRRGLRLYLPCVASTFVSFWFMHNDWGYPPLVKGGFWPELAHCFHELWVFASQPWNWDRRQDPPYDGHLWTIPVELSMSMLLFITITGLSRCKTWVRLTVTMAIIWYTLQCKHWAPAEFLAGALIAEFGLIQDEYKNAHAVALDPATDVEKDSDEFQINLPSSTKNAIGKAWEIFWWCQLVIALYICGWPIAKAHEVPFFRWLQQRSPDNPCPDTFWFIPVSFLIVGACHQLAPLQKILTTPFVQYWASISYALYLMHGPVMHAVYFTMEPIFRMNGGKDGAGFWGYHLQWLAGLFLTGIPIVWASDLFWRFVDQPCVDMARWLDRKCLDKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCC7</accession>
    <name>A0A8H6CCC7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Endocytosis protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HO133_003092</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the PAN1 actin cytoskeleton-regulatory complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the END3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150094.1">
        <property type="nucleotide sequence ID" value="XM_037294016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCC7"/>
    <dbReference type="GeneID" id="59331504"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025604">
        <property type="entry name" value="End3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12761">
        <property type="entry name" value="End3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EH" evidence="20">
        <location>
            <begin position="10"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="42"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="20">
        <location>
            <begin position="142"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="299"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF6220659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="411" mass="46293" checksum="8F0DE97E8D776C71" modified="2022-01-19" version="1">MSNKRIEQAEVEKYWEIFSPLANGGTHLSGDQAASVLKNSQLREDQLARVWDLADVDNDGNLDFEEFCVAMRIIFDLVNGEYADVPSSLPDWLVPESKAHLVQATRALSGNQPAFSPISPSDDDDDTPGLKDGFDWYMSPSDKSKYESIYTANKGPHGALTFQSLESLYASLDVPDTDLRSAWNLANPASEPAIGKHAALAFLHILNNRHEGYRIPRSVPASLRATFEQGQIDYNPENRSVKSRSPAQRWGYEDEDTSTGRKAKFGDTYLSRLGVGGKSTYQPSGTNFSSTRTTEDWEEVRLKRQLQDLENKIDTIESRAKGKSGRKREEESKPALVKRELEMLLDYKRKEIRELERGEGRSKAGEALKGVENEIKGVREMVEGLEAHWKTREGVLDDLRRQIEDEKHSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCF3</accession>
    <name>A0A8H6CCF3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_002689</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the engrailed homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150443.1">
        <property type="nucleotide sequence ID" value="XM_037293614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCF3"/>
    <dbReference type="GeneID" id="59331101"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050720">
        <property type="entry name" value="Engrailed_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24341">
        <property type="entry name" value="HOMEOBOX PROTEIN ENGRAILED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24341:SF6">
        <property type="entry name" value="HOMEOBOX PROTEIN INVECTED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="60"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="62"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="272"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="381"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="663"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="685"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="710" mass="77415" checksum="65CB7E8E8B01F87F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCH6</accession>
    <name>A0A8H6CCH6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002473</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150228.1">
        <property type="nucleotide sequence ID" value="XM_037293399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCH6"/>
    <dbReference type="GeneID" id="59330886"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054471">
        <property type="entry name" value="GPIID_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF15">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22939">
        <property type="entry name" value="WHD_GPIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="5">
        <location>
            <begin position="207"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase winged helix" evidence="4">
        <location>
            <begin position="481"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="846"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="879"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="835"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="982" mass="110464" checksum="9859364DDF0AC7D8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCS1</accession>
    <name>A0A8H6CCS1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150425.1">
        <property type="nucleotide sequence ID" value="XM_037293596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCS1"/>
    <dbReference type="GeneID" id="59331083"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1020">
        <property type="entry name" value="Prion-inhibition and propagation, HeLo domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029498">
        <property type="entry name" value="HeLo_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038305">
        <property type="entry name" value="HeLo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542">
        <property type="entry name" value="HELO DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542:SF1">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14479">
        <property type="entry name" value="HeLo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="234"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="611" mass="67986" checksum="2B798B4D5945F6ED" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD55</accession>
    <name>A0A8H6CD55_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP-1 complex subunit sigma-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Sigma1-adaptin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_002089</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037293016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD55"/>
    <dbReference type="GeneID" id="59330503"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000994">
        <property type="term" value="F:RNA polymerase III core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016482">
        <property type="term" value="P:cytosolic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016480">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14831">
        <property type="entry name" value="AP1_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000007">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1000.50:FF:000004">
        <property type="entry name" value="Repressor of RNA polymerase III transcription MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.50">
        <property type="entry name" value="Repressor of RNA polymerase III transcription Maf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044733">
        <property type="entry name" value="AP1_sigma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015257">
        <property type="entry name" value="Maf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038564">
        <property type="entry name" value="Maf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504:SF0">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09174">
        <property type="entry name" value="Maf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="12">
        <location>
            <begin position="3"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="424"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="425"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6221234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="492" mass="55971" checksum="25A9A790F0B7CA34" modified="2022-01-19" version="1">MAINYLILLSRQGKVRLAKWFTTLSPKDKAKIIKDVSQLVLSRRTRMCNFLEYKDTKVVYRRYASLFFIAGCSSTDNELITLEIVHRYVEQMDKYYGNVCELDIIFNFQKAYFILDELLLAGEMQESSKKNVLRCISQQDSLEDMEYLPLRDIDDVTNALNFDTADCHVIGGCDLYTTKAAGADKKLYKNIEHSLESQYASLLSFSAGLSAHQATAAGQALNLSRASPFGPLSQISSRRTFAYLIATLNASHPDYDFSHLLRPSDFRREKNIRKVMNTLDTTLYNLRPKFSSALVDEKPHWSNTVPTTVPQTPHGSEKWNPRMWSLIDKEMTLKECSMYCYAPEEDPYDGEEGSLWSFNYFFFNKAKKRVCYIYLRGLSIIGNSPPQKTPIKPKRPASGTWSLDEETSKKRAKYWLGDRAREAEWNNEEDEDVSGQWDEENIEEADILDDGDEPTLVPSDTDTGSESPCKGRGRSGSSVRGVSEEVAATIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD75</accession>
    <name>A0A8H6CD75_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell wall proline rich protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150742.1">
        <property type="nucleotide sequence ID" value="XM_037293089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD75"/>
    <dbReference type="GeneID" id="59330576"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="824"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="896"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="366"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="838"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="928" mass="99125" checksum="84AD36ADAF0DB3EE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDU8</accession>
    <name>A0A8H6CDU8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151029.1">
        <property type="nucleotide sequence ID" value="XM_037292490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDU8"/>
    <dbReference type="GeneID" id="59329976"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034303302" evidence="1">
        <location>
            <begin position="20"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="226" mass="25665" checksum="6F8524D19688A65A" modified="2022-01-19" version="1">MHSFFRLPLLLHVISVVVGGVIPYLWTVDPTSLEPDRDLTEVIPSANNTALTQPSHANLSDHISFVDVIYDGVKLVRSRYSRAVFREAKAASIVGPATDPHYLTDMRLVFSRTQGDYRSVYLEMTETWPRWGEPRLTTNVPPGDYGVLPSRFGMDIVDADRRMKAAGFGQRYKAVDIRWPAWFPEEWQQALYFFQMDDDGLDFVTVGARDGRVIPFDHALDAAEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE02</accession>
    <name>A0A8H6CE02_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150891.1">
        <property type="nucleotide sequence ID" value="XM_037293238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE02"/>
    <dbReference type="GeneID" id="59330725"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="93" mass="10616" checksum="8896AC6AC5A19ECB" modified="2022-01-19" version="1">MDTNPITRRYGPEPQKPKNSGTLLNQTRARLERAQSLFHVGSSVWEIWVGGRQERRVVSDCTWDEGQGYVVMEESAMKDLGWLEEYSLTSDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF98</accession>
    <name>A0A8H6CF98_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151586.1">
        <property type="nucleotide sequence ID" value="XM_037292167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF98"/>
    <dbReference type="GeneID" id="59329653"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="856" mass="94074" checksum="DCFDCAD04D620BB3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFA1</accession>
    <name>A0A8H6CFA1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase S1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001508</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151857.1">
        <property type="nucleotide sequence ID" value="XM_037292438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFA1"/>
    <dbReference type="GeneID" id="59329924"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF64">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034954347" description="Carboxypeptidase S1" evidence="7">
        <location>
            <begin position="21"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="596"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="597"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="621"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6222422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="632" mass="68335" checksum="DC98A7EF43ABBE59" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFE6</accession>
    <name>A0A8H6CFE6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151671.1">
        <property type="nucleotide sequence ID" value="XM_037292252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFE6"/>
    <dbReference type="GeneID" id="59329738"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6222236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="56" mass="6233" checksum="4A7532A8226F2390" modified="2022-01-19" version="1">MRVTQDIRLKVIAGAKLCARGGIIKSRHEAESYTGRRTKAASHEASHVCLESVQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFE7</accession>
    <name>A0A8H6CFE7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000613</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151914.1">
        <property type="nucleotide sequence ID" value="XM_037291552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFE7"/>
    <dbReference type="GeneID" id="59329032"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="465" mass="49665" checksum="9B9FE8D81758B4CE" modified="2022-01-19" version="1">MVRISVLYRIPLAGKTFILFPKRTVYLAYLGVFELGSLVCALAPTSNALIAGRAVAGFGASGVFAGGFVLLTTIIPLHKRAIWTGTMSSTFAIASIVGPVIAGAFTEHITWRWCFYLNLPIGGFAAVLILFLFRIKAAATEKARLLEKIKGLDGLGFVLFAGSITMLLLALQFGGTAYAWKSSVIIGLFVGFGVTMLAFIPWQLYLQDTALIPPRLFKNRNASLIFSSSIFVNGPFQTIVYWLPIWFQAVLGVSPEASGIRYLPTVISDALASMIGAGIVMQLGVWNPFLLFAEVMVCIGGGLLTTIHPGISDGHWIGYQIFGGIGYSLASNLAHLGMQASLPQDLVPIGATTLLFGISTSCAIFLSIGQTLFNSRLSTNLSHVVPPDIASNVMSVGATNVRSVVGSGDLTAVLQAYSNAVTQVFYIPAVAPVISFLLLLGCTWTSVKKPKAKEPQEESEPKENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFK8</accession>
    <name>A0A8H6CFK8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000692</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151991.1">
        <property type="nucleotide sequence ID" value="XM_037291629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFK8"/>
    <dbReference type="GeneID" id="59329110"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF14">
        <property type="entry name" value="CAFFEOYL-COA O-METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22458" checksum="156E60E2933C3C6A" modified="2022-01-19" version="1">MPPATTTQMSTEGRGDYETDSRWTSIDAYTMSHLHPPSRPNHSALTRTLRACDAHGLPDIGCPSPLGKSFALQCRMLGVRRALERRTRPSAPGADVLPRLHAEVESGARERFGFAFIDADKVNNHRYFDWAVRMSVPGACICVDNVVSKGKLAMAEEAARNDMVRGARECVERVGADERVEATVLQTEGEKDYDGYVCSAAFGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFP1</accession>
    <name>A0A8H6CFP1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001410</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151759.1">
        <property type="nucleotide sequence ID" value="XM_037292340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFP1"/>
    <dbReference type="GeneID" id="59329826"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF4">
        <property type="entry name" value="AMIDASE C550.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="171" mass="17966" checksum="F3A395108B66E506" modified="2022-01-19" version="1">MHLETSSNLYGVTVNPFNRSSTSGGSSGGEGSLMGLRGSCLGVGTDVGGSIRSLAANNGLFGLRSTTSRIPLMGKTSLKIGSGHVQGTLGPLSTSLEGVKLFMKTVLAAKPWITDPGLIPIPWRDEESYFQSDGYKKIKIAVLWSDAVVNPHPPINRALKEVVNGLEAIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFQ4</accession>
    <name>A0A8H6CFQ4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein phosphatase PP2A regulatory subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000724</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152023.1">
        <property type="nucleotide sequence ID" value="XM_037291661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFQ4"/>
    <dbReference type="GeneID" id="59329142"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000011">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="166"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="205"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="246"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="279"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="285"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="324"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="363"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase 2A Regulatory Subunit A helical" evidence="5">
        <location>
            <begin position="367"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="402"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="519"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="582"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="616" mass="68680" checksum="5C3AEE9A09D990B8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG18</accession>
    <name>A0A8H6CG18_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A to I editase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151927.1">
        <property type="nucleotide sequence ID" value="XM_037291565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG18"/>
    <dbReference type="GeneID" id="59329045"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043829">
        <property type="term" value="F:tRNA-specific adenosine-37 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042935">
        <property type="entry name" value="Tad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47803">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47803:SF1">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A to I editase" evidence="1">
        <location>
            <begin position="54"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="406" mass="45076" checksum="B85DD8E59FEA2393" modified="2022-01-19" version="1">MGTLEDRAVIAIHEKFHGLPRKSKPRTFTNGSQEWVPLSGIVVIGEDGAITCLALGTGMKCLPGSKSLLLDSGTVLHDWHAEILAIRAFNYFLLQECHQLASCANYTSPILRRRQARELSEVQNLQPFSIREDLRIMMYCSEAPCGDASMELVMEAQADPTPWPVTASNEKAASGFLGRGSFSQLGVVRRKPSRADSPITLSKSCSDKLALKQCTSLLSSPVSLLISPGNAYIDTLILPYHRYRPEACERAFGPAGRMLPVDNSTWLAGYEFRPFRVETAEIDFHYSRRVAGTNLNGRKGSNISAVWTPRHQETLINGVLQGRKQTDPLGASALSRMQIWNLLVETIGLIDALPPRNFLKMSSYLDMKKSDGLEHRRRVKDDVKSEALKGWSNESSNTLFEEAVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG92</accession>
    <name>A0A8H6CG92_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="15">PARP</shortName>
            <ecNumber evidence="15">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_000693</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ARTD/PARP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151992.1">
        <property type="nucleotide sequence ID" value="XM_037291630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG92"/>
    <dbReference type="GeneID" id="59329111"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01437">
        <property type="entry name" value="parp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.142.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.140.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.140.10">
        <property type="entry name" value="WGR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF60">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WGR" evidence="19">
        <location>
            <begin position="44"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="PARP alpha-helical" evidence="18">
        <location>
            <begin position="167"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="17">
        <location>
            <begin position="306"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6222646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="542" mass="60824" checksum="B57F04543A0E31A5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGJ6</accession>
    <name>A0A8H6CGJ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001034</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152329.1">
        <property type="nucleotide sequence ID" value="XM_037291967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGJ6"/>
    <dbReference type="GeneID" id="59329452"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF9">
        <property type="entry name" value="OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="28"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="330" mass="36771" checksum="3A6D2A49A8AEE56E" modified="2022-01-19" version="1">MPAPPVPASLQIIVNATKVEYVQLVQLTKGLSTWDTANVYSSGINEEIIGKAIQKFALPRHKLTILANCCGTVPDEPGIFSWPFEARMQKSKDYVNQGGEQLCVNMRRLSRGAILKAVDASLKRLGKDYIALLQIHMYDASVPPEETMKALHDLVQAGKVNYIGASSMWTYQNARMQFIAEKNGWTNFVSMQNRYNLGYSEEERGMNKFCNETGVGLIPWGHLFSGLLAKPWGVESARSKMSVAMSGGLTEADEIINKRVQEVAERKGWKMNQVALVWIREKGCIPIVDLNSTSIERLDEACAVRDMTLSADEVKYWEGPYAPKPIAGHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH55</accession>
    <name>A0A8H6CH55_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_000234</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152609.1">
        <property type="nucleotide sequence ID" value="XM_037291174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH55"/>
    <dbReference type="GeneID" id="59328653"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1590.10">
        <property type="entry name" value="glutathione-dependent formaldehyde- activating enzyme (gfa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337:SF40">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337">
        <property type="entry name" value="GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="5">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6223392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="141" mass="15693" checksum="674FBE9F78393018" modified="2022-01-19" version="1">MPIYKGGCYCGEVKYTIDVSPDDARTSLCHCKNCKKFFGTAFGLTTKVPVSSFSYTSESKKPTIHEADNGSGTTLHREFCPTCGSGILEVGAAAMKDFRYIMTGTLDHPGDLPPKGEFWCKRREKWMPEVPGIFHKQEIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHG3</accession>
    <name>A0A8H6CHG3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MAK16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000173</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MAK16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152548.1">
        <property type="nucleotide sequence ID" value="XM_037291113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHG3"/>
    <dbReference type="GeneID" id="59328592"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.110:FF:000001">
        <property type="entry name" value="Protein MAK16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006958">
        <property type="entry name" value="Mak16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405">
        <property type="entry name" value="MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405:SF4">
        <property type="entry name" value="PROTEIN MAK16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04874">
        <property type="entry name" value="Mak16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003352">
        <property type="entry name" value="MAK16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="6">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="203"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="329" mass="38055" checksum="A5E5EFAE634E3B3F" modified="2022-01-19" version="1">MASDEIVWQIINQQFCSFKLKTPKQQNFCRNEYNVTGLCNRQSCPLANSRYATVRSNPETGTIYLYMKTIERQHMPSKLWEKIKLSQNYGKALEQIDARLIYWPKFLIHKCKQRLTRLTQVAIRSRRLAQEEEILGEKVVPKLAPKVRRREATRERKAEAAAKVERAIERELIERLRSGAYGERPLNVEEGVWKKVLKGLERGGDGERDKDLDQGIEDEEELEEEEELEEEGVGQVEYVSDIDEEEDVELEDFEDWLGSGEETGNDEEDGDSEEESGSEDEDEKEMKKPATGAKRKRGKAPQSARKTKPRMEIEYEDEANLPVREAVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHK2</accession>
    <name>A0A8H6CHK2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450 alkane hydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_000553</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152927.1">
        <property type="nucleotide sequence ID" value="XM_037291492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHK2"/>
    <dbReference type="GeneID" id="59328972"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11063">
        <property type="entry name" value="CYP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002974">
        <property type="entry name" value="Cyt_P450_E_CYP52_ascomycetes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002402">
        <property type="entry name" value="Cyt_P450_E_grp-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF1">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00464">
        <property type="entry name" value="EP450II"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01239">
        <property type="entry name" value="EP450IICYP52"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602402-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6223710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="59852" checksum="B6390AE73766DEFA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIC0</accession>
    <name>A0A8H6CIC0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010704</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153190.1">
        <property type="nucleotide sequence ID" value="XM_037301556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIC0"/>
    <dbReference type="GeneID" id="59339094"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02642">
        <property type="entry name" value="R3H_encore_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051937">
        <property type="entry name" value="R3H_domain_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 21 RELATED R3H DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672:SF8">
        <property type="entry name" value="PROTEIN ENCORE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12752">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="3">
        <location>
            <begin position="259"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="SUZ" evidence="4">
        <location>
            <begin position="323"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="95"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="364"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="567"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="657"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="416"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="674"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="725"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="750"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="770"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="808" mass="87704" checksum="8798D875BAE7BA63" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIK4</accession>
    <name>A0A8H6CIK4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">High affinity methionine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010825</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153310.1">
        <property type="nucleotide sequence ID" value="XM_037301676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIK4"/>
    <dbReference type="GeneID" id="59339215"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000025">
        <property type="entry name" value="High-affinity methionine permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF382">
        <property type="entry name" value="LOW-AFFINITY METHIONINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="58530" checksum="AD9E83DD9E64DC73" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIP1</accession>
    <name>A0A8H6CIP1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Threonine synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010731</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153217.1">
        <property type="nucleotide sequence ID" value="XM_037301583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIP1"/>
    <dbReference type="GeneID" id="59339121"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1380.10:FF:000003">
        <property type="entry name" value="THR4p Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="entry name" value="Threonine synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051166">
        <property type="entry name" value="Threonine_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690">
        <property type="entry name" value="THREONINE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690:SF1">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Threonine synthase N-terminal" evidence="5">
        <location>
            <begin position="9"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6224157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="194" mass="21453" checksum="75A8456C36A7EDA5" modified="2022-01-19" version="1">MSSHTTSQRYLSTRGGSYGFSFEETVLKGLATDQGLFLPEDIPSLPSNWQDDGSHMSFSELAFQFFSLYIAPSEIEPVALRNIINRSYSTIRAPSVTPTVTLNNARTIHLLELCHGETFAFKDIFFRVIRNEGKSGKDREHLTVLCAPSGDTGSAAIYGLRGKKDVSVFVKYPTGKVGPIQEAQMTTVTDRNVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIZ5</accession>
    <name>A0A8H6CIZ5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Polyketide synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010884</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153367.1">
        <property type="nucleotide sequence ID" value="XM_037301733.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59339274"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049492">
        <property type="entry name" value="BD-FAE-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041068">
        <property type="entry name" value="HTH_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF21">
        <property type="entry name" value="NON-REDUCING POLYKETIDE SYNTHASE AUSA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20434">
        <property type="entry name" value="BD-FAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22621">
        <property type="entry name" value="CurL-like_PKS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18558">
        <property type="entry name" value="HTH_51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="10">
        <location>
            <begin position="385"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="11">
        <location>
            <begin position="1284"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="9">
        <location>
            <begin position="1648"/>
            <end position="1724"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="7">
        <location>
            <begin position="1284"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="7">
        <location>
            <begin position="1431"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1590"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1722"/>
            <end position="1759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1631"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="7">
        <location>
            <position position="1315"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="7">
        <location>
            <position position="1494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2496" mass="271397" checksum="BE42D94FCD8ED714" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJS9</accession>
    <name>A0A8H6CJS9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_011076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153559.1">
        <property type="nucleotide sequence ID" value="XM_037301925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJS9"/>
    <dbReference type="GeneID" id="59339466"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="160" mass="18533" checksum="C9DF453FAB99F3FE" modified="2022-01-19" version="1">MRQQTTSLPPLRDLDQPFVWDIRMYNRSYRFEFSDTASPEHYTLLKPDFIVLCYDINDRRTLVNVQHVWFKRMVESYMKDRDDIPIMLLGLKRDLREEKPGVIYPQEGLKAAQDLRCDRYAECSATTGELMNEVIEDIARTAAKTTTEAGALSNGGCTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKL4</accession>
    <name>A0A8H6CKL4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154124.1">
        <property type="nucleotide sequence ID" value="XM_037301307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKL4"/>
    <dbReference type="GeneID" id="59338845"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="387" mass="42757" checksum="C11AC3C343FAE88E" modified="2022-01-19" version="1">MVDIHTSTALNDPPSKDGASMPIPNLRRPRMLPNKIGQCERTEKAIEIELLQGWKKKNVILELTNRILEWNDTLCPNCQANLKGKGPQVLDEHEVNNYEGRDLPDADFESAAHTNDAEDSNCSEAVTSLRKILGKGTQRDLSLSSPDCNQRLVAFLTIQTSASVAISVLALLPSHPTAAIYHSALDTPCMANAPAPGWLRQQPAKTDPDIFDDEDTAPESPAAARALRACNSRQRSDTRRVKNSTPPNSGCAAAMPVADAPFPSTLGAFIGCNQRQNSDETPLQPHEHSLNCALAQMQLRIRQNQYTSPGGAQMWESDVARFNELVTSTYGKRALGWCEIARHTIEEERASQAHNVRQKDAVIGELRQNVDRLRREVAELKDMVPWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKN6</accession>
    <name>A0A8H6CKN6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Thiamine phosphate synthase/TenI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_010204</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-(2-hydroxyethyl)-4-methylthiazole + ATP = 4-methyl-5-(2-phosphooxyethyl)-thiazole + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24212"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17957"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the C-terminal section; belongs to the Thz kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the N-terminal section; belongs to the thiamine-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153876.1">
        <property type="nucleotide sequence ID" value="XM_037301059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKN6"/>
    <dbReference type="GeneID" id="59338596"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00139"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004417">
        <property type="term" value="F:hydroxyethylthiazole kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01170">
        <property type="entry name" value="THZ_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000042">
        <property type="entry name" value="Probable thiamine biosynthetic bifunctional enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000104">
        <property type="entry name" value="Thiamine biosynthetic bifunctional enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00228">
        <property type="entry name" value="Thz_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00097">
        <property type="entry name" value="TMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000417">
        <property type="entry name" value="Hyethyz_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034291">
        <property type="entry name" value="TMP_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006830">
        <property type="entry name" value="PRK09355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00693">
        <property type="entry name" value="thiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00694">
        <property type="entry name" value="thiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20857:SF23">
        <property type="entry name" value="THIAMINE BIOSYNTHETIC BIFUNCTIONAL ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20857">
        <property type="entry name" value="THIAMINE-PHOSPHATE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02110">
        <property type="entry name" value="HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01099">
        <property type="entry name" value="HYETHTZKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="Thiamin phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine phosphate synthase/TenI" evidence="18">
        <location>
            <begin position="7"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6225009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="517" mass="54067" checksum="F80F3982D4314CD9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL34</accession>
    <name>A0A8H6CL34_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154236.1">
        <property type="nucleotide sequence ID" value="XM_037300388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL34"/>
    <dbReference type="GeneID" id="59337922"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="281" mass="31339" checksum="EF7058788AE14525" modified="2022-01-19" version="1">MAETSTDPAPDHAGAAYPKPAGWEYMTHEERYAALRSEPKPDSEYLQKALAQAKQDVLRDERDERKAITEARQITVLADAPHFTISLTAPPTLSLAQGIYPIAAILTYTSDPASTTFTTQAARPVLFRPCYGPLSPSAPNENLYSVYTTPTCTPECRIPHVRPNASIRPPREADGSFTKELEVTSWDGWEEAHLGDTVRKEVAMGLDERSGWSKHLETGKSYWLRCDDAGFLGVGRLGLDRYWRYGDKDSMELPMKIKLRDPEAVSIPLEPSNVVEFQVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL70</accession>
    <name>A0A8H6CL70_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009503</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154212.1">
        <property type="nucleotide sequence ID" value="XM_037300364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL70"/>
    <dbReference type="GeneID" id="59337898"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="89" mass="9833" checksum="4081356E70016444" modified="2022-01-19" version="1">MSRPFVQNFLSVLVHSVYPAGLAGLGVAGDRYLLGSQLEKVEVKLGSRMDAVKDDLGIVKKDVTGLVDMERRKVDVVERMLDCGKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM04</accession>
    <name>A0A8H6CM04_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009867</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154574.1">
        <property type="nucleotide sequence ID" value="XM_037300726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM04"/>
    <dbReference type="GeneID" id="59338262"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025066">
        <property type="entry name" value="CCDC174-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15885">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15885:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13300">
        <property type="entry name" value="DUF4078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="11"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="251"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="323" mass="35671" checksum="847B79F166F07ED7" modified="2022-01-19" version="1">MTSNPNLYGLPRPRTSKPTKPTPSSNAAFTSALSSLLALPSPSTTTTPGRARPSRSKPDIFTAHNKGAKKRALADISGPQSHARTSDAVDAATLHRSKRKMEDKARLYASMKRGDYVPPADGGTNKEENALVDFDRKWAEADEAGEAGRYETESDDGGDSVDDGGEGEERVEYEDEFGRLRHGTRASASRELRRRDAAAHAEEELGRMRARPARPEGLIVGDTIQAAAFNPAAAGMEALARKRDRSLTPPEEVHYDAGSEDGEGRRREMDALGREREETERGRREGVERRERRRGEVEERKRVIAKKRGERLAERFLNGLDVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM27</accession>
    <name>A0A8H6CM27_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_009006</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154693.1">
        <property type="nucleotide sequence ID" value="XM_037299867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM27"/>
    <dbReference type="GeneID" id="59337401"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11040">
        <property type="entry name" value="CYP7_CYP8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6226140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="466" mass="53566" checksum="926074CA1441D7A3" modified="2022-01-19" version="1">MAGTVIIREYFHNTRRPFRLTLAGENLYVLTSPHDVAAAYKETESLTFDQFIRDIMVAFGASPGAIEKMWLLPSNDKHGFNTQVKNPSQKSLAQLTRDFHKQQLHPGQNQRSLSVKFQDYIDESLRWDSMSIKLNDKYTLRSSEMSKDIWLKGWCADVLLNAATRAFYGESLLQNQPDLFREFFDFDDNSWMLMYRYPRFMASSMYQAKDAAIDALTQYFATPKGSRRGEAWFIRTLEAEQRQLGIGDRDIATLSLMVYWVVNSNTYKLCFWILTYLLHDSTLLALIREETSHAFTTTGELSMTFLLDSCVRLNCLFDEVLRITNSSSSVRSVVSDTIIGDFVLRSGAKVLIPYRQLHFDEAIFGTNAQEFDSGRFLRQRNLHQSPNYRPFGGGSTYCPGRFIARQEVVAFVAFVLKRFELEIPNAEKDGIPNTFPRLEEMKPCLGVMGPVQGNDLIVRIKRRMCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMF1</accession>
    <name>A0A8H6CMF1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154643.1">
        <property type="nucleotide sequence ID" value="XM_037299817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMF1"/>
    <dbReference type="GeneID" id="59337350"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="75" mass="7925" checksum="881518DFA4C670FB" modified="2022-01-19" version="1">MPGALKSDLNRHMPKLVQLLLLWTLQEDPTFGAYTEAFAGLSPKVDQGRNGAFGFGDGSGGGGWNETCGEILGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN05</accession>
    <name>A0A8H6CN05_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009395</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155082.1">
        <property type="nucleotide sequence ID" value="XM_037300256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN05"/>
    <dbReference type="GeneID" id="59337790"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="9">
        <location>
            <begin position="11"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6226529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="307" mass="34389" checksum="AEC642E3D591D7BC" modified="2022-01-19" version="1">MPPTNHPPHQPRVVLYHQTHYHRNNFVSLLPLLTEAADLVPITHLIIAAIHLNDPPGHIHLNDDPPDSSKYDSLWEEVAIMQDIGVKVLGMLGGAAPGSFQRLDKSDGEFEAYYIPLRNMIRQHNLDGLDLDVEEAMSLNGIIRIIDRLKADFGPDFLVTLAPVATALQGRPHLSGFDYEALEIMRGSSIAWYNTQFYNNWGRLESFIDYDAILRQGWRPEKVVVGVLTSPGNGNGFVDTEAFKRTVTAMNTFVVLGQDAQLPVLDRGGTRLAKARRMPPIQQTDRSRETRSRDCDGMEITVTTKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CN79</accession>
    <name>A0A8H6CN79_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155048.1">
        <property type="nucleotide sequence ID" value="XM_037300222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CN79"/>
    <dbReference type="GeneID" id="59337756"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36459:SF1">
        <property type="entry name" value="FATTY ACID DESATURASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36459">
        <property type="entry name" value="ORF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="2">
        <location>
            <begin position="155"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="466" mass="54140" checksum="23F6B653D7F4C0D6" modified="2022-01-19" version="1">MYRYITSTRKTLWVPAVSGSGCSTVIGLSPLHPPNRMDASINPLLLKPDLIVLQNLLQDIRRTNSHHPADSSAAHNDQSSMRALDEVHDETTVFTSWDLKDLPAGKGFRADILGKYITWAQGIVRNPVDVVFLTHIILYFSTSIPSAVFLYYHFTWAHGIFHCLLQLSYCGSFTLMLHNHIHNNGLLKKKYVWFDNTWPYILEPLMGHTWDSYYYHHVKHHHVEGNGPEDLSSTIRYQRDELSNFLMYVGRFIAFIWIELPLYFLRKRKPGLAFKAAASEMAAYLFIYLAARRNFYPTLFVLIIPLFLMRIGLMVGNYGQHALVDDVDPASDFRSSITLIDVPSNRHCFNDGWHTSHHLNPLRHWKDHPRAFLKAKNEYTNGRALVFQNVDYLMMTYRILAKDYLYLADCVVPIGDQIGMSRQELADMLRTKTRRFSEEEIQEKFYKGRKTQKEAKKKDDLYISKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP12</accession>
    <name>A0A8H6CP12_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="59336784"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="282" mass="29082" checksum="8DB146F937F4ABF9" modified="2022-01-19" version="1">MSSAPQLTARDVNLLAIAFQSLKDDCALQIDYAKLAQLAGYKTAASANACFLTLKKKLMAGASVSNAAAAPTPKKAKGKAAANGDADDDDEATPSKAAPKKRGKAIKTELADAEGGDADTEVTPGAESPKKGRAKKRTGPSKTDANGDTPTKATTNEDATNDTAATGITTPTPTPTPKRKRGPSKPKDPNATPTKRAKKNANAKVTTSADGAAADDNNDAANAQLHGVETVAQTNGEGSIFGGDANVKEEEQEGDDRCFDAEEQKMAEDELFNIYTTEGQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP39</accession>
    <name>A0A8H6CP39_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037299421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP39"/>
    <dbReference type="GeneID" id="59336951"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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        <location>
            <begin position="19"/>
            <end position="533"/>
        </location>
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        <location>
            <begin position="222"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="533" mass="54669" checksum="A7D20B162233B07E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP67</accession>
    <name>A0A8H6CP67_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Domain of unknown function at the cortex 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="Duc1"/>
    </dbReference>
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        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826:SF2">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08588">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="36"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="342"/>
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    </feature>
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            <begin position="183"/>
            <end position="197"/>
        </location>
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        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="342" mass="38444" checksum="D347C17FA9AAB1B8" modified="2022-01-19" version="1">MLNRSISRSHAHNPLHVVVQFLFTHFASDMADKYLLRVTAGSSYDTTTHQTVQVNKPTPTHISTAICDAKISVRIQNYRGLPRKSPTTSAYFSHALHLHDQYSIAFTLLPHKSFPGSALVFGNSFDRPIRDRLPPGTSTALRIVKWAIDPGLDGDLYSDTPHLYGNALSSVNILRVGEKVKDGKDLPEPVGEEKALEEGGDGEGEEWRKSKDVPETGDARKKHFLTKGKTEEWEWEEGRVYECDFFNPYLDFNSFSLKLPGFSLSVLPYLGGTDYLRYVLKIKDTDEVLFVVVFSLLHKEDVEKEEAEPKKGGEDTKVTSQESQGARHEGEKDFEPEADDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPE1</accession>
    <name>A0A8H6CPE1_9LECA</name>
    <protein>
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            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
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            <taxon>Pezizomycotina</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="59336841"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23703">
        <property type="entry name" value="mS26_PET12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058940">
        <property type="entry name" value="mS26_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26163">
        <property type="entry name" value="mS26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="271"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="308" mass="35144" checksum="D1A962E373427F70" modified="2022-01-19" version="1">MPPRAVGTQRCLRPRQCIHHTKSPGFSTSSPSRAQIPPESPKFIEVPRLVQPKQPFPVRMKGVLPPPRDIFHRRKEGKIPRASPEYFAAVTPEPLEDKTTRSDDEFVSWKARQSELRRRNLREGLAELHQRRQESDRRRSIISAANTRIREKRLYAPERKDVRLTNPSVLSTQQPTKHRALPDPTRPGRLAVMSQNVADMAAMREEARRNALHTLYVNAGDFITTGKQLDEVVDRAFDDNSQFRTDSSEGLNIWNLGFPETVQELLGKANKTGRQTALDSAEGNEITTRERMKKIGEELTGGKMLEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQ20</accession>
    <name>A0A8H6CQ20_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008588</name>
    </gene>
    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
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            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155454.1">
        <property type="nucleotide sequence ID" value="XM_037299453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQ20"/>
    <dbReference type="GeneID" id="59336983"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="37360" checksum="E8E44412F237CBA7" modified="2022-01-19" version="1">MDLPDNTSTTSSSSSSRSTVSDVAESLSSVALLHPNNEQKTMTGFPPEIILQIYRHLNSASDITALNSTSRRYNSIWRINAASISAAVLSRSIDCYNSAFELFEIEERVKQIYCIILPQSAVLKRLRLAQKQAQDVVRQGRRNDRCDHTSSDILYRGVLYRNEELLSAARCASYLLRLIEDRVVYTGGTSLDELDREITPSSHHIIVAFHELMILIRLRLLEAMQARLKNMRKEKIRKMLYVATYLVRDCPDKKKIRLGISREKIRRSSMVDDMDLAFRPRCYVIVHARRAFFAIADAVGEARIPNYLLDNRSGCHGDCEDIGKIEGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CR51</accession>
    <name>A0A8H6CR51_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155981.1">
        <property type="nucleotide sequence ID" value="XM_037298645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CR51"/>
    <dbReference type="GeneID" id="59336172"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05188">
        <property type="entry name" value="MDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF4">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="3"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="291" mass="30389" checksum="34D9C5A0F822C54F" modified="2022-01-19" version="1">MLGSAGWKQHGIKASPIQPSDILNSKGLFPHTSFPRTPGRDFAGVVVDSSSRDIGKEVCGTSGHDFSFTEDGCHAEYCLISEKAVVEQPKKLSFAQAASATTPFTTAAIALRRAGVKAGETVLVLGATGQVGSAVVHLANLWGCKVLGVARGNAAEIDSAGDPTLSKAKALTNDKGPDVAVDAVGDAELAQAAIEVLGFRGRYSFIPAPGGGNSKVPVDFLSVYRQEVTLVGNNSVEHSQEELAQELKSMCEGFGSGALVSMKESAMIFISLQEAIEGYADGKKRYVISLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRG2</accession>
    <name>A0A8H6CRG2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Alternative oxidase</fullName>
            <ecNumber evidence="17">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HO133_007923</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 17">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="16 17">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the alternative oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="17"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156127.1">
        <property type="nucleotide sequence ID" value="XM_037298791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRG2"/>
    <dbReference type="GeneID" id="59336320"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009916">
        <property type="term" value="F:alternative oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010230">
        <property type="term" value="P:alternative respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01053">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.140:FF:000002">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.140">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002680">
        <property type="entry name" value="AOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038659">
        <property type="entry name" value="AOX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803:SF3">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01786">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005229">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 16 17" id="KW-0408">Iron</keyword>
    <keyword evidence="14 17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 17 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005229-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF6228193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="279" mass="32456" checksum="3D80EAA232AA0B16" modified="2022-01-19" version="1">MAPPDMNAIQVAHREKKTWSDVWALSMVSLLRFGMDTATGYSHPTEEAKKMGSWLGLSKVKSWFGPSPMTERKWLIRFLFLESVAGVPGMVAGSVRHLHSLRRLKRDNGWIETLLEEAYNERMHLLTFMKMAEPGRFMKTMIIGAQGVFYNMFFMSYLFFPRACHRFVGYLEEEAVLTYTRAIHDIEAGRLPKWQKLEAPEIAVKYWNMPEGHRTMRDLLLYIRADEAKHREVNHTLGNLEQKNDPNPFVSEYEDPVRPHPSKGIENIKPTGWNREEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS60</accession>
    <name>A0A8H6CS60_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156305.1">
        <property type="nucleotide sequence ID" value="XM_037298969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS60"/>
    <dbReference type="GeneID" id="59336498"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034347691" evidence="2">
        <location>
            <begin position="23"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6228371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="256" mass="25955" checksum="C062DB00E8E7DF23" modified="2022-01-19" version="1">MAPFTVFTALAGILALSTSVSAQTGTGTGTGLPIASATLGAPYPLSNATLPAGPAGTGIGLPTGLGSPSALPFSLPGTSPSGLPGSPIESGASCPVPITVTTTNQVTVTVTVIPSSAAAPTTPASVPYPIPGSSGLLVGTGIVTRYGTGTGTGTGTGYAMATGYSKRMEMRGLKQERREWKRGASDIEARQRMKNVTMKRDQMFQRQSSIFHNLPAGPLPHTTILISSVSSHDETNDLTPSSTGGIKFLPNQGVSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS61</accession>
    <name>A0A8H6CS61_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Vacuolar transporter chaperone complex subunit 4</fullName>
            <ecNumber evidence="7">2.7.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Polyphosphate kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">SPX-dependent polyphosphate polymerase VTC subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Vacuolar membrane polyphosphate polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">HO133_007986</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[phosphate](n) + ATP = [phosphate](n+1) + ADP</text>
            <dbReference type="Rhea" id="RHEA:19573"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19574"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Vacuole membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the OST1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the VTC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.1" evidence="7"/>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156190.1">
        <property type="nucleotide sequence ID" value="XM_037298854.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336383"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07751">
        <property type="entry name" value="PolyPPase_VTC4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14480">
        <property type="entry name" value="SPX_VTC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.100.30:FF:000001">
        <property type="entry name" value="Vacuolar transporter chaperone 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 23" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034567825" description="Vacuolar transporter chaperone complex subunit 4" evidence="23">
        <location>
            <begin position="20"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1220"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1263"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="24">
        <location>
            <begin position="473"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="503"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="570"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="992"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="1006"/>
            <end position="1022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00075894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAF6228256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1282" mass="145488" checksum="BE3C30352FD9079B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS68</accession>
    <name>A0A8H6CS68_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carotenoid cleavage dioxygenase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008234</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156438.1">
        <property type="nucleotide sequence ID" value="XM_037299102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS68"/>
    <dbReference type="GeneID" id="59336631"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF24">
        <property type="entry name" value="CAROTENOID ISOMEROOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="434"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="568"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="62415" checksum="381580440308F44B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS71</accession>
    <name>A0A8H6CS71_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156320.1">
        <property type="nucleotide sequence ID" value="XM_037298984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS71"/>
    <dbReference type="GeneID" id="59336513"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="244" mass="27507" checksum="46FA0C11DAC6C923" modified="2022-01-19" version="1">MIGFEITNAMDCLSISEPRKVPGLPVRPSWLAEESMNREADISKAVQGIMTTWNAWTDCVEKVFQQMGNQTADLQISEDEYINFVHMFASHPARPIRRPLEDITEHEYWFMRTMPHRYLTYLHGLWNDYADRLEKTYIGMAAELGRCNLLASPFETASPRGQLHGILRSMLGPGRLFHGLADTRTHEVVAVGEMSLQGRSFTHSDQLVSGIETMGLHGAPATYMKGAEELVVGLKNMHIEADIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6M9</accession>
    <name>A0A8H6F6M9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006682</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147015.1">
        <property type="nucleotide sequence ID" value="XM_037297579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6M9"/>
    <dbReference type="GeneID" id="59335083"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1144" mass="125507" checksum="05090E1F1DEA0A90" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7G8</accession>
    <name>A0A8H6F7G8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146983.1">
        <property type="nucleotide sequence ID" value="XM_037297782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7G8"/>
    <dbReference type="GeneID" id="59335286"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="488" mass="54746" checksum="141E9C0C4813139C" modified="2022-01-19" version="1">MAASKAAPKATRKATRKATRKATRKAASTATSTVASKTASRAACHFLRIPIEIRLKIYGYLLLDPPRASLKPNQYEHTWQHHFGPEDEDMDSGSMGYGSFHTDVYGDSDSEDAMFDLDDFNPYGIPMGDMCDGMFNDVYNLGGAMSMTFGNDMFDYGDDHSDDRGFDRMDDEDESENESGPSRGMFETEPMIKRHLAILRTNRQIYNEASVLLHSDLTLDVNPGDAVTDVPGNAVVKQTKKIWRHSPHMGLGLTDSNGQTVYKSCPLDGSVEPHVFARFERISYNAQFFFEGDDAAPCLFINDDLSVRADDAAKFVSYLTTAKGITRWFEDPMSGRPFDNGRRETLKDVADITISSVTVTQPSTAEIIQKFVDLVSKSPLIRHLEVNLDLEVRCENWIESLKWGPDTEQTAKNDEKRDAANERAAELFIESGVLDSLRKLSNVKCFSLKVTTVWIGETVMLLQQKHLNIIRDLKDVIEKNWAVKHGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7J2</accession>
    <name>A0A8H6F7J2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glutamate decarboxylase</fullName>
            <ecNumber evidence="3 9">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_006666</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3 9"/>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146999.1">
        <property type="nucleotide sequence ID" value="XM_037297563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7J2"/>
    <dbReference type="GeneID" id="59335067"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000017">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.160:FF:000004">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.50:FF:000001">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321:SF6">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="472"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6217564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="516" mass="58120" checksum="B49F6D5F29727ECA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8B5</accession>
    <name>A0A8H6F8B5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005706</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147794.1">
        <property type="nucleotide sequence ID" value="XM_037296613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8B5"/>
    <dbReference type="GeneID" id="59334111"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051714">
        <property type="entry name" value="Znf_CCHC_NABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23002">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="293"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="469" mass="50480" checksum="5CD8F17715EAFA10" modified="2022-01-19" version="1">MSDWNAAAGGAEWDSGAGAAGNAWTDGVDAGASAGGDFGTTNGDATQKGAGAGGDFTCRRCGEAGHMARQCPTAPEGSGNDGKCFNCGEEGHSKVDCPNPRVFKGTCRICEKEGHPASECPDKPPPKCFNCKQEGHQTSECKANRVFDTSRVADMSAEDAWEALQKADEERELEDFREVRGNKRCLYKRNINFSHQAFRVYTKVASDATYDELETAFRVNNFNTYLIATENELLKTHTYVNLQGELDKTYKVGFHLSPKPKRETAKQGWPETPEENLERLKDAGLPMERGIPKCIRCDELGHTARACPEEAVENTDRVQVKCVNCEEVGHRARDCPTPRVDRFACRNCKWDISAKTAPSLVTIAKSSVLIVTKVCRRASYACTELHFADKKAVGHTKVRCKEPIREVDDGGFGAGGDTGGFDAPVSGEDFAAPAAGGVDEWAAGSGGATDEWGTAPAATTVGGGGQDNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8E3</accession>
    <name>A0A8H6F8E3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004907</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148517.1">
        <property type="nucleotide sequence ID" value="XM_037295819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8E3"/>
    <dbReference type="GeneID" id="59333313"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6219082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="83" mass="9482" checksum="923D9BCA2A1775A1" modified="2022-01-19" version="1">MAQQKKATQADQAMEMPDVNVETAAMFTKNARWVSPDTNKTDTRTYIIEAHLQYLKHVHHTPLVIDPSNFENAVKSMPVEDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8J4</accession>
    <name>A0A8H6F8J4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Heme haloperoxidase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_005057</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the chloroperoxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148667.1">
        <property type="nucleotide sequence ID" value="XM_037295969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8J4"/>
    <dbReference type="GeneID" id="59333463"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.489.10">
        <property type="entry name" value="Chloroperoxidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000028">
        <property type="entry name" value="Chloroperoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036851">
        <property type="entry name" value="Chloroperoxidase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33577:SF1">
        <property type="entry name" value="HEME HALOPEROXIDASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33577">
        <property type="entry name" value="STERIGMATOCYSTIN BIOSYNTHESIS PEROXIDASE STCC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01328">
        <property type="entry name" value="Peroxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47571">
        <property type="entry name" value="Cloroperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51405">
        <property type="entry name" value="HEME_HALOPEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034517619" description="Heme haloperoxidase family profile domain-containing protein" evidence="8">
        <location>
            <begin position="21"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Heme haloperoxidase family profile" evidence="9">
        <location>
            <begin position="79"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6219232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="443" mass="47080" checksum="256CD0330E786B98" modified="2022-01-19" version="1">MMISKLPFVLFALSAIQVTAFPHLSVNGVEELAELAKKSGECPHLAKQNQAACPHLAKNKDLKRQTTFDPTSQHVSTTGEYAWVAPGAGDQRGPCPGLNALANHGYLPHNGVADIPTIVSAVNTAYGMGIDIGTFLAVYGTAFDGDSISTDPGFSIGGPATSEEILGVGLLGTPSGLSDSHNNYEADTSPTRGDLYVVGNDYLLQVDRFQEYYDALPENTPAPDQYAALFPFRQAQFNNSVATNPDFFFPQFAGVFVSPAGYAFPPRMMANHSAEYPSGYLDKASFMTFFAVTGESGAFEYTPGWERIPNNFYRRPLSDPYTLPDFILDVLAFGLLDPRLLSIGGNTGTPNSFTGLDVSDLTDGVFDGATLAQGNNLECFILQLVQAEAPTAVAALYEDVTAALQPLAESISGNLAGLGCPQLGAVNAGMYDVYPGYAKANGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8N2</accession>
    <name>A0A8H6F8N2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148139.1">
        <property type="nucleotide sequence ID" value="XM_037296958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8N2"/>
    <dbReference type="GeneID" id="59334460"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.10">
        <property type="entry name" value="Diaminopimelate Epimerase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003719">
        <property type="entry name" value="Phenazine_PhzF-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00654">
        <property type="entry name" value="PhzF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774:SF32">
        <property type="entry name" value="ANTISENSE-ENHANCING SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774">
        <property type="entry name" value="PHENAZINE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02567">
        <property type="entry name" value="PhzC-PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54506">
        <property type="entry name" value="Diaminopimelate epimerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="348" mass="38146" checksum="12DFC95EB5C7B7F5" modified="2022-01-19" version="1">MNTFEQRLQQLASNSNSREATPRPPTTTTNSLPYSTLDVFTTTRFKGNQLAVVHVPSSFSLPASVLLSQETKQTIAREFNFSETVFLHDALVETSDRRLDIFTLKEELPFAGHPIIGTICHVCQSEEPPLESVTLLCKAGPIVGRYDPETRFAEAEIPHNVRIHQAPVSGRAVLKAQPYLSRSSVVTNELRVVSIVKGLSFVLIQLPSISPHLEKLEISGHSVDCDSVELDEGWTPSFIGTYFYAITSQTSEKVVRLRTRMLEPSVGEDSATGSAASTLASFLALQDGTAEMTFYYSIEQGVEMGRASEIQVEVTLDATGKAVEKVVLSGRAVHVTQGVLYLPERTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8P1</accession>
    <name>A0A8H6F8P1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_005276</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148169.1">
        <property type="nucleotide sequence ID" value="XM_037296187.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333682"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034034665" evidence="3">
        <location>
            <begin position="26"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="448"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="519"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="914"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1246"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="249"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="568"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="729"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="459"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="480"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="923"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="991"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1048"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1115"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1129"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1246"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1270"/>
            <end position="1286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6218734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1335" mass="146809" checksum="B833BF80D7627860" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8T2</accession>
    <name>A0A8H6F8T2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_003986</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148952.1">
        <property type="nucleotide sequence ID" value="XM_037294905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8T2"/>
    <dbReference type="GeneID" id="59332395"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180:SF63">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE FAMILY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03520)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="281" mass="29959" checksum="95A99BAC2C203A57" modified="2022-01-19" version="1">MASTKRGLRLEGKKAIITGAAGGIGLETTILFAREGAAVLMADISEPALATALAKVHTILPDPPRLETIKCDVSKESDVEAMVKHVDSWGGVDIMFNNAGIMHGDDADAIDTPEKIWDLTHNINVKGVWFGSKHAVLSMRRHEKKVGSIINTASVVALVGSATPQLAYTASKGAVLAITRELAIVHAKEGFRFNALCPAPLNTALLQDWLGDDAAKRHRREIHFPSGRFGEAIEQAQAVLFLASDESSFVNGTDFVVDGGLTRAYVTAQGPATKPPANFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9A3</accession>
    <name>A0A8H6F9A3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003867</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149477.1">
        <property type="nucleotide sequence ID" value="XM_037294789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9A3"/>
    <dbReference type="GeneID" id="59332278"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034661237" evidence="2">
        <location>
            <begin position="16"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="205" mass="22863" checksum="B51E668395629116" modified="2022-01-19" version="1">MLFILLLFFALAALAAPFDPLLDLDTIKNLTATNRCSSSQDWQAYAFLVEDCFTAIQRVYLEQVLQKPDEIYEFIAPGSYPQTRKPWVRTPMQYTTNSCTLSIVMLNWFGPREPLPGRGPRGYEPSDTATFKDIYGAARVVERDCLLPTRRPGWDVVGAKSSIGVFLWATDSVINDRVTGQESLIHSSPNASSSSNNANNIDSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA44</accession>
    <name>A0A8H6FA44_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003440</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149743.1">
        <property type="nucleotide sequence ID" value="XM_037294362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA44"/>
    <dbReference type="GeneID" id="59331851"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030639">
        <property type="term" value="P:polyketide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF46">
        <property type="entry name" value="TYPE III POLYKETIDE SYNTHASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000451">
        <property type="entry name" value="PKS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="4">
        <location>
            <begin position="99"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="5">
        <location>
            <begin position="295"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="444" mass="48491" checksum="D46A5F309BB22A9E" modified="2022-01-19" version="1">MAAAEVSTANGGIAPQLTFEPRDSYQLPLVGPNMDTKQDYSVTVHEDVSTTPVSTTPSKETPGLYITGLGSQYPPFQFPPDKLEGFVKRWYDLETPGIKKLLTINQTTMIQSRSTIKAYEDPFWSRSVAPSISELDRFFRKAGVDLTVQACKKAMRESGSKPSDITHIVAVTCTNAGNPGFDLLVAQKLGLKPETDRTLLHGVGCAGGLSAMRAASAMAQSASVRGRPARILVFACEICSINVRCDLEEVVEHPDETKISPVLFSDGAASFVLSNELTPGAKDKAVYSLVNWETRTMPGTAKELEFMADPFGFRATLTKEVPHLALKAITPMFEQLLPFLPTDSQLATSGKPVEAKDFDWALHPGGIAILNGAQQKMQLHDDQLRASFDVYKNHGNSSSPTVLVVLDQLRKMGEGRENVMACSFGPGMTIEMFMLKRCRHDVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA67</accession>
    <name>A0A8H6FA67_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150437.1">
        <property type="nucleotide sequence ID" value="XM_037293608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA67"/>
    <dbReference type="GeneID" id="59331095"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047865">
        <property type="entry name" value="Ribosomal_uL10_bac_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="360" mass="39586" checksum="1B92629E04B72704" modified="2022-01-19" version="1">MPPRLRICPQCYHLLKHQPTSYRYLATASAITPAAPPFQTISSPPPIARFPRTQPPSHKPPEFRKSQLHRQYTSLLRSTPLLLLFQHNNLKATEWMSIRRELQFALEKVDNDLSTNLAPAIKIQTIQVGIFESALLVAEYFDPKQHRTASKPHPTDPSTQSSVSLANHTPTPADPTFRHYLSRTAHAAASPYKESHPLRPLLSGPLAAMSFPTVTPQHMRAALSILAPKAPTFPAPTRRANPGYHDATVQQGLQKLMLLGARVEGKVFDNEGARWVGGIQGGMEGLRGQLVRILGEVGAGVTGALEGMGRSLWVTVEARRSMLEEEEKGTSGGSNEGLANKGKEHSIGLWSVIWGRNPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAF9</accession>
    <name>A0A8H6FAF9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150014.1">
        <property type="nucleotide sequence ID" value="XM_037293936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAF9"/>
    <dbReference type="GeneID" id="59331424"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12809">
        <property type="entry name" value="Esterase_713_like-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050228">
        <property type="entry name" value="Carboxylesterase_BioH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194:SF4">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194">
        <property type="entry name" value="HYDROLASE ALPHA/BETA FOLD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="40367" checksum="90D6C649EF6B79BD" modified="2022-01-19" version="1">MPTTSAERVFHAIPVPATAESLYNRSYFYVGGEYVDAESGDGQRIFTGQMYVEQLTPAGGVKHPWPIVFIQGGGQTGTNFLNTPDGRKGWASWLLDQGYTVYLLDQISRGRSSYHPSTGPTITYTAELAEKRWTACRDFQLWPEAALHTQWPGSGRVGDPVFDAYYASTVPSLRDYSVEQTLMQTAGASLLDRIGPAVLATHSQGGTHGWLWADVRPELVKAIVAIEPSGPPFQSTIVKDATVKLYGITDIPMSYDPPADATIDGQAPLQTHEQPALNGRTCILQQEPARRLKNLSKIPVLLETGEASSHASYDEFTVRFLRQAGVNVKHLKLADEGVHGNGHLQFLEKNNLEIIEMLEKWIKTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBQ0</accession>
    <name>A0A8H6FBQ0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151696.1">
        <property type="nucleotide sequence ID" value="XM_037292277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBQ0"/>
    <dbReference type="GeneID" id="59329763"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021842">
        <property type="entry name" value="DUF3435"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37535">
        <property type="entry name" value="FLUG DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37535:SF3">
        <property type="entry name" value="FLUG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11917">
        <property type="entry name" value="DUF3435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="765"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="766"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="808"/>
            <end position="819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="819" mass="93090" checksum="565369C23F93F215" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCF0</accession>
    <name>A0A8H6FCF0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152070.1">
        <property type="nucleotide sequence ID" value="XM_037291708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCF0"/>
    <dbReference type="GeneID" id="59329189"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034171313" evidence="1">
        <location>
            <begin position="23"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="329" mass="36152" checksum="517BF43925C8EA99" modified="2022-01-19" version="1">MRFHTHQVFGALLGVCVWVAVARSVLDSKRFTPEERELSANSYDEYADIEARNASLLFARDTDGPMADDTPPGFGCRSMCGIGTPSTSSKRQLEDRSWLQKRVFEPFPPTPNTQKARDQYMITHVDGLQTGQEIVPYEDNVDDYATAVTGALGTTTVTFGMRGICGCTWLAVISQKRVYLGHYWESLSFGKPEGATYRLQFATQVTNFLTTGYKGQPSLASVAADFQGDPGLQAYIYTPESEAKPGTQEYPDKIAKMVSTVNALVGVEPTVRTYDAQDAKTPGGQKILETTGAGHMLFNYDPTGGSRAKPNAWRILWESQVVAVGQWKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCL4</accession>
    <name>A0A8H6FCL4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sulfate adenylyltransferase</fullName>
            <ecNumber evidence="9">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-sulfurylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Sulfate adenylate transferase</fullName>
            <shortName evidence="9">SAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MET3</name>
        <name evidence="13" type="ORF">HO133_000999</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>sulfate + ATP + H(+) = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Allosterically inhibited by 3'-phosphoadenosine 5'-phosphosulfate (PAPS).</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Homohexamer. Dimer of trimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The adenylyl-sulfate kinase (APS kinase) is non-functional. It is involved in allosteric regulation by PAPS. PAPS binding induces a large rotational rearrangement of domains lowering the substrate affinity of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCL4"/>
    <dbReference type="UniPathway" id="UPA00097"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00204"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010134">
        <property type="term" value="P:sulfate assimilation via adenylyl sulfate reduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.10:FF:000003">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000802">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000052">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03106">
        <property type="entry name" value="Sulf_adenylyltr_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027535">
        <property type="entry name" value="Sulf_adenylyltr_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050512">
        <property type="entry name" value="Sulf_AdTrans/APS_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004040">
        <property type="entry name" value="PRK05537.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="12">
        <location>
            <begin position="4"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="11">
        <location>
            <begin position="174"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="APS kinase" evidence="10">
        <location>
            <begin position="395"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric regulation domain; adenylyl-sulfate kinase-like" evidence="9">
        <location>
            <begin position="395"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="197"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="291"/>
            <end position="294"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="434"/>
            <end position="437"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="site" description="Induces change in substrate recognition on ATP binding" evidence="9">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6222948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="573" mass="64034" checksum="5EA78FB0206D126D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCT4</accession>
    <name>A0A8H6FCT4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000453</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152827.1">
        <property type="nucleotide sequence ID" value="XM_037291392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCT4"/>
    <dbReference type="GeneID" id="59328872"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054471">
        <property type="entry name" value="GPIID_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF15">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22939">
        <property type="entry name" value="WHD_GPIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="4">
        <location>
            <begin position="210"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase winged helix" evidence="3">
        <location>
            <begin position="480"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="690"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="723"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="756"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="789"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="822"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="896" mass="100930" checksum="E9D7296EC30DF1FC" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDK1</accession>
    <name>A0A8H6FDK1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_010497</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the RNase T2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152983.1">
        <property type="nucleotide sequence ID" value="XM_037301349.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338887"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01061">
        <property type="entry name" value="RNase_T2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000004">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022099">
        <property type="entry name" value="DUF3638"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022105">
        <property type="entry name" value="DUF3645"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046541">
        <property type="entry name" value="DUF6606"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033697">
        <property type="entry name" value="Ribonuclease_T2_eukaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF33">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367">
        <property type="entry name" value="UBIQUITIN THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12340">
        <property type="entry name" value="DUF3638"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12359">
        <property type="entry name" value="DUF3645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20255">
        <property type="entry name" value="DUF6606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034570275" evidence="15">
        <location>
            <begin position="24"/>
            <end position="3561"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6606" evidence="18">
        <location>
            <begin position="366"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3638" evidence="16">
        <location>
            <begin position="2418"/>
            <end position="2638"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3645" evidence="17">
        <location>
            <begin position="2760"/>
            <end position="2792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2215"/>
            <end position="2257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="3533"/>
            <end position="3561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="2215"/>
            <end position="2246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="2247"/>
            <end position="2256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="3551"/>
            <end position="3561"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR633697-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF20255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6223923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="3561" mass="402442" checksum="58D134FD262CFC8B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE03</accession>
    <name>A0A8H6FE03_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010202</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153874.1">
        <property type="nucleotide sequence ID" value="XM_037301057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE03"/>
    <dbReference type="GeneID" id="59338594"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030638">
        <property type="term" value="P:polyketide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006973">
        <property type="entry name" value="Cwf_Cwc_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718:SF2">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04889">
        <property type="entry name" value="Cwf_Cwc_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07366">
        <property type="entry name" value="SnoaL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="237"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="308"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="402" mass="45473" checksum="480BA0DE1104528B" modified="2022-01-19" version="1">MSSWQPPIKVSTAQTDEEKKNIAVVLDYMSTAYSPENNKGRSSVSHLCTPNSTFAAPTTFPTCHTPLDYADSHAKVMSAIADLHIISYDIVFAKEGHVLLRYSAEGSHCGQPHNGIEPSGKKAKWTAAAIFEVQDGKVHSFTKEWDKLNMWKQLGWMKGDDIRNIYASLKMTTAHRPTFDPARGKDAQRGVAYHQRLLPAHTQLKVRQPGQGGDADPYVRDLRAQLLEAEAVHFSKTNPAAAKTTESTISSKRRLEDGPGENGEEEDLQAKRLRVLEETRNVDADSDGSKSDDSSEEDSDDEEDETAELLRELEKIKRERAEQKEKEERERASAEQEKRENDIALGNPLLMPTKETETKRRWDDDVVFRNQARGTENKGKKEFVNDLLRSDFHKRFMGKYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE50</accession>
    <name>A0A8H6FE50_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_010354</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154024.1">
        <property type="nucleotide sequence ID" value="XM_037301207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE50"/>
    <dbReference type="GeneID" id="59338745"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13965">
        <property type="entry name" value="PT_UbiA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050475">
        <property type="entry name" value="Prenyltransferase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723:SF1">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6225157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="360" mass="39623" checksum="7792DCCBC6593DCC" modified="2022-01-19" version="1">MGDPTMSDATEKHNNIPSPVCVRPFVLKRTNFIFSESSQQIKNNRDDKKNETLLQYRLKTLWLFTFSDLKTIVVPSTAFAILTALSGPSLTTNSSVPSLSELFPRLPLVLAWIWLTLLLVDIANQRHPGSVLEDSLNKPWRPLPSGRISTYGARRLLLIAIPSVLAITKFFLPQGLPVTIIAIIGSYMYNDLGGADESFLVRNLMNAAAITCFGAGAAQVAIGEQVALNVDAYRWLAIIAAIVTTTMQVQDMEDQEGDRAKGRVTIPLLLGDVTARHSIAVLVSAWSFVCPAYWGLNALGYVLPGAVGLTIAGRTLTMRNVQADKDNWRAWNIWMMTLYLLPVWEDIFSTVASNEIEDGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FES1</accession>
    <name>A0A8H6FES1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AA1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154065.1">
        <property type="nucleotide sequence ID" value="XM_037301248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FES1"/>
    <dbReference type="GeneID" id="59338786"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034227460" description="AA1-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="17421" checksum="79C24036D664E05F" modified="2022-01-19" version="1">MRLISPSLLASLALAAPYVSGQACSKHQSWNISELAIFTAAPDSESSDLIDFAVVDPLVDYHVNPSESWNYFCQRELPAGNAGSLADPVDYYTCIGSPTQFKWDGLRLSLKEVVECEGNNVTATGTSKPVFATDCYATDANGADGEWCLATYDFNITITFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF67</accession>
    <name>A0A8H6FF67_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154585.1">
        <property type="nucleotide sequence ID" value="XM_037300737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF67"/>
    <dbReference type="GeneID" id="59338273"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="86" mass="9323" checksum="DFEF5BCF2F24F2EE" modified="2022-01-19" version="1">MADQTYGGNSLAVAENPPTQTSTRREFTMQPHTPRSQTPGRTQTLARNSRGTLSRLPECGGDDGEGRLHDRQADTGAETADGDHER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFM5</accession>
    <name>A0A8H6FFM5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_008963</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154651.1">
        <property type="nucleotide sequence ID" value="XM_037299825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFM5"/>
    <dbReference type="GeneID" id="59337358"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2320:FF:000001">
        <property type="entry name" value="phenylalanine--tRNA ligase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2330:FF:000002">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000028">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040725">
        <property type="entry name" value="PheRS_DBD3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18553">
        <property type="entry name" value="PheRS_DBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="18">
        <location>
            <begin position="223"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="271"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6226098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="517" mass="58874" checksum="5DD2CB30B95296D7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FH36</accession>
    <name>A0A8H6FH36_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155835.1">
        <property type="nucleotide sequence ID" value="XM_037298499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FH36"/>
    <dbReference type="GeneID" id="59336026"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="352" mass="38125" checksum="2A2892EF8311CAC5" modified="2022-01-19" version="1">MPDLKLVGVETRAPLATYTSRIAQLHQASVQRANWLPRKITKSLLSVAGGKAADSRCGCEARAAHSDTKGTPMHDSIANQAPRYDTYDGVHIESEAATKPNESNFTPTGNTVPSQTVETTHDEEEAIEISSTSTAAGSSITFTTNLEYFSGFFRHRITQYRQRECGSLLRSSDSALSNSPGTASDISTGSTDSILNRLFKQFDHVHGIVKQESRERLRQERDRFRQDRLNYLNGVPSHMGLPSNTGYAPPVNQLLELNPSDSASHHVGSARVSTAPAPEAASQVDSRPPWSVNSGGFDQTSSAPCGHPSNVSPQSLSNQFASMPPSFPPRTSDRSNAMAASGLSPRQEQLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI94</accession>
    <name>A0A8H6FI94_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156654.1">
        <property type="nucleotide sequence ID" value="XM_037298019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI94"/>
    <dbReference type="GeneID" id="59335526"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="422" mass="43214" checksum="96EE2B566E6BC733" modified="2022-01-19" version="1">MLISISTSPSVYIKYSTLVANYDCDTYSAPNLGTIYDEVTIGYPPDALSTGVCYHTDWRSIDYTELYYPPPDHEVVTQAACSMDYRDPRTVDSSKISAGSPIFSIPAGVSKVDPLWSTCTPAAPRRYDPPRALNKTRNPVPTPTPPSRRGVGNGGNSGSTPHYNGGNVGSGPAEGSKANGADPQDPGVSTAPDSASEGNPGSVSPQNDSNGGSEIGASLQEAPGGGLELGTVTIAPGAETTHSGHVVSVGSSLVLVDGTSFDLQSPHPGAPPGLAYTSSPPQQGVSGGVVVMGTMSLPSGTQTIFSGHTMSVGSNIFGVDGTSYRKGASGGLVFGTVTLPPGSEITQSGPVIPVGSSSAVVDGTLCTWAAPASSPTSTPASELLTTMMVTHNVLLCFLDIQAGMMLHINLLALFEDLFLIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIJ0</accession>
    <name>A0A8H6FIJ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_007321</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156847.1">
        <property type="nucleotide sequence ID" value="XM_037298212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIJ0"/>
    <dbReference type="GeneID" id="59335720"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF11">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="5">
        <location>
            <begin position="57"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="241"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000443-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6229205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="459" mass="50700" checksum="6CFD9662B7366C88" modified="2022-01-19" version="1">MTAQLDHIPEKRNPYVDLISDQVFALISSLDLDSGHVLEKCEVAYKTWGVLNEKRDNVLVVCHALSGSSDLEDWWGPLLGPGKAFDYTRYLVFCGNLLGSPYGSASPLTINPSTGRPYGPEFPETSIRDDVRAHKLVLDAMGVQSVAAVVGGSMGGMSALEWPLCTPVGYVNTIIPIATSADHSAWGISWAEAQKQCIYADSNYQDGYYDMGSQPTKGLAAARMVALLTYRSGVSFDKRFGRKSPAKRKGLQKSKEDLADGHCGNGVNSNSQNPKCDAFRSRKRAQIEASNRTQTRTHRTRSPVFSAQGYLKYQGDKFIRRFDANCYINLTNKMDTHDLTRDRIVRGEETDEVEEAFTEVLKAVPAKALVVSVDTDNLFQPEQQVKLAKYLPDATLSIIRSEDGHDGFLLEFEALDGLISERLKQQCSWVYKDPPLLESNGTGSAAVKDSVFGEVESEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIK6</accession>
    <name>A0A8H6FIK6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein BCP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007311</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Involved in nuclear export, actin cytoskeleton organization and vesicular transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the BCP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156837.1">
        <property type="nucleotide sequence ID" value="XM_037298202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIK6"/>
    <dbReference type="GeneID" id="59335710"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025602">
        <property type="entry name" value="BCP1_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261">
        <property type="entry name" value="BRCA2 AND CDKN1A INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261:SF0">
        <property type="entry name" value="BRCA2 AND CDKN1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13862">
        <property type="entry name" value="BCCIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028983">
        <property type="entry name" value="BCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="291" mass="32984" checksum="F5FA9A414D53684F" modified="2022-01-19" version="1">MAKRKEIDNNRGGELKSEESSSEESSSDDDTNMLDVEFEWFDPQPEHDFHGLKTLLRQLFDVDAQSFDLSALTDLILAQPLLGSTVKVDGNETDPYAFLTVLNLHEHKDTPVVEKMITYIRAQAKRTGALSEIDDLVENAANARIGLVLTERLVNIPSEVVPPMYKMLLEEISWAIEEKEPYDFTHYLILSKTYREIASALDEEDTPKSKKQKKASKKGTSEVFYFHPEDEVLQRHALCSGSYEYLVEGAKSDSKRTFQELGIKPEGHMILIVANKFEAAVKGLQEYFSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIP8</accession>
    <name>A0A8H6FIP8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20 21">Cytochrome b-c1 complex subunit Rieske, mitochondrial</fullName>
            <ecNumber evidence="3 21">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">HO133_007352</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="21">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="21">The Rieske protein is a high potential 2Fe-2S protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rieske iron-sulfur protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="3 21"/>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156878.1">
        <property type="nucleotide sequence ID" value="XM_037298243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIP8"/>
    <dbReference type="GeneID" id="59335751"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03470">
        <property type="entry name" value="Rieske_cytochrome_bc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.270:FF:000002">
        <property type="entry name" value="Cytochrome b-c1 complex subunit Rieske, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit Rieske, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.270">
        <property type="entry name" value="Ubiquinol cytochrome reductase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037008">
        <property type="entry name" value="bc1_Rieske_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004192">
        <property type="entry name" value="Rieske_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02921">
        <property type="entry name" value="UCR_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81502">
        <property type="entry name" value="ISP transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12 21" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 22" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 22" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 21" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rieske" evidence="24">
        <location>
            <begin position="156"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00072517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAF6229236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="249" mass="27585" checksum="827BBFDF7A48CF32" modified="2022-01-19" version="1">MPPLPAARSTLLRLCPRQHVQSTITPSILAATQQRRCKADIVERHSGEYEQTPRFESPFKNRDENPTTKIPSFANYMSKKGETSNKTFQYFMVGGMGLLAAAGAKATVQDFLVNMSASADVLAQAKVEIDLAAIPEGKNVIIKWRGKPVFIRHRTADEIKEAEDTKWESLRDPQPDSDRVKKPEWLIMLGVCTHLGCVPIGESGDFGGWFCPCHGSHYDISGRARKGPAPLNLAVPEYDFSEDEKVVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIZ5</accession>
    <name>A0A8H6FIZ5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157075.1">
        <property type="nucleotide sequence ID" value="XM_037298440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIZ5"/>
    <dbReference type="GeneID" id="59335948"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="183" mass="20240" checksum="8F11DCB4D2CE5ED4" modified="2022-01-19" version="1">MVLELGGFIELDTEICPPCTARNPRNGKLAQCIEKDEVIVFELRPRKVLEFLCTNVPSPMVTVTARDIWRNATVQFKILEPAQIVDLVGTRQALGVSCGTLQLVNCSTRYRLHANIEHDILQRETNRTASGTTSDCMEVRFKVIGVGMGVTLAHSLDLRKDRGTQSVAPSATGDIRIEMDAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK38</accession>
    <name>A0A8H6FK38_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157228.1">
        <property type="nucleotide sequence ID" value="XM_037295227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK38"/>
    <dbReference type="GeneID" id="59332717"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF16">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="2">
        <location>
            <begin position="437"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6229971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="733" mass="82297" checksum="75D8392905CA782E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKP6</accession>
    <name>A0A8H6FKP6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_004617</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157534.1">
        <property type="nucleotide sequence ID" value="XM_037295533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKP6"/>
    <dbReference type="GeneID" id="59333024"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178:SF4">
        <property type="entry name" value="FAD-DEPENDENT MONOOXYGENASE APTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178">
        <property type="entry name" value="MONOOXYGENASE, FAD-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="8">
        <location>
            <begin position="296"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6230277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="370" mass="41223" checksum="4AD79A4D9A2CBB80" modified="2022-01-19" version="1">MSLPRITTVVGAGLGGLILGQCLKAKNIPATILEKASSSSRFNYGVTLQQSVYQPLLRILQMNEASFLEKCSIGVPEVQVNSATTTTTIRCHRGRLEGILREGLDIRWEQCLKSVKMTPQEISLHVENGPTIASDTIIGADGVHSQLRKSFIPSSCLNVLPYVVFNGRRSITLQDYKHGLQQHMAGQTIIHALRGNVLFRVYVNEYTATHVCLGYTYSRPARANDQLYEPYRPTTGAENIPEEFYAELSLFKQKELGQGFADIFDSEKVRQDRVLHWLMRSSTVPLGEIQDLAVRGVWLIGDAAHPTPILGGEGANKTITDAIELVDHLSNGSASNKNEFLEKRHQEWRRVLNEGERRLSEMHGLGFPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKX6</accession>
    <name>A0A8H6FKX6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_004764</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157679.1">
        <property type="nucleotide sequence ID" value="XM_037295678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKX6"/>
    <dbReference type="GeneID" id="59333170"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196:SF1">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6230422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="37866" checksum="4234E6D54C67BC6D" modified="2022-01-19" version="1">MGHRKRTWPLQPSTHTAAPLQVPGTPQQSSPQHLGTSPVKMANMSNLYRDPRSIIATHHDTYPYISPSKFTGSLNGKVILLTGAGRGIGRASALAFAAAGANVACVSRTHSDTLSLVHEISQKGHPRAIAIAADVADADAPGRVVGEVQGELGPVDVLVNNAGISRISDVEHEHGMGAAMEVVEVNVRGALNFIHAVVPSMIQRKRGVIINVVSVLATISLPYFSAYSAAKAALVRSTHIMDLEFRPHGIYSYAVHPCMSRDTSLGEGALNSEAHEKVEGVKRFMEDFVPSMTDTVQLPANTFVALAADPDAKYMSGKYIDSTQDLGMVLEEARKGSGSRIEREGLYILKVDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL16</accession>
    <name>A0A8H6FL16_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157739.1">
        <property type="nucleotide sequence ID" value="XM_037295738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL16"/>
    <dbReference type="GeneID" id="59333232"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF8">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="2">
        <location>
            <begin position="114"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="551" mass="63108" checksum="30EEDC8FC2CEEF0C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYR5</accession>
    <name>A0A8H6KYR5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007303</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156829.1">
        <property type="nucleotide sequence ID" value="XM_037298194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYR5"/>
    <dbReference type="GeneID" id="59335702"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF33">
        <property type="entry name" value="PROTEIN HOS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="64"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="826"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1065" mass="117578" checksum="6AEE0476879B99CA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZM0</accession>
    <name>A0A8H6KZM0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit OST2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004392</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DAD/OST2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157310.1">
        <property type="nucleotide sequence ID" value="XM_037295309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZM0"/>
    <dbReference type="GeneID" id="59332800"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003038">
        <property type="entry name" value="DAD/Ost2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02109">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6230053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="136" mass="14748" checksum="9CDC8E3A86D68A81" modified="2022-01-19" version="1">MPAKRAPQISPHASAVPPIPKPSPKTSAAPPSSPAAQTAREVSSALWWRYRSATPQRVKLTDAFMAFLVAVGGLQFAYCVLVGNYPFNAFLSGFGATVGQFVLTASLRIQTNPENRREFESVSHERKRAFLGETEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5K1</accession>
    <name>A0A8H6C5K1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycogen debranching enzyme</fullName>
            <ecNumber evidence="5">2.4.1.25</ecNumber>
            <ecNumber evidence="6">3.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Glycogen debrancher</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HO133_006850</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolysis of (1-&gt;6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.</text>
            <dbReference type="EC" id="3.2.1.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="2.4.1.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the glycogen debranching enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.25" evidence="5"/>
    <dbReference type="EC" id="3.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000027">
        <property type="protein sequence ID" value="KAF6217512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146947.1">
        <property type="nucleotide sequence ID" value="XM_037297746.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59335250"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004135">
        <property type="term" value="F:amylo-alpha-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11327">
        <property type="entry name" value="AmyAc_Glg_debranch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000039">
        <property type="entry name" value="Glycogen debranching enzyme Gdb1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000242">
        <property type="entry name" value="Glycogen debranching enzyme Gdb1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010401">
        <property type="entry name" value="AGL/Gdb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032788">
        <property type="entry name" value="AGL_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029436">
        <property type="entry name" value="AGL_euk_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032792">
        <property type="entry name" value="AGL_glucanoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032790">
        <property type="entry name" value="GDE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006421">
        <property type="entry name" value="Glycogen_debranch_met"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01531">
        <property type="entry name" value="glyc_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569:SF2">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06202">
        <property type="entry name" value="GDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14701">
        <property type="entry name" value="hDGE_amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14702">
        <property type="entry name" value="hGDE_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14699">
        <property type="entry name" value="hGDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Eukaryotic glycogen debranching enzyme N-terminal" evidence="18">
        <location>
            <begin position="42"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme glucanotransferase" evidence="19">
        <location>
            <begin position="142"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme central" evidence="20">
        <location>
            <begin position="754"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme C-terminal" evidence="17">
        <location>
            <begin position="1088"/>
            <end position="1542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF14701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF14702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF6217512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1555" mass="175302" checksum="E0B124D448B05542" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C5V9</accession>
    <name>A0A8H6C5V9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147042.1">
        <property type="nucleotide sequence ID" value="XM_037297606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C5V9"/>
    <dbReference type="GeneID" id="59335110"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="18300" checksum="47ACFF9E876B4C37" modified="2022-01-19" version="1">MPCRSIKDSQSKALRAWYNDDSTPGTGLNSKKTLEDCGRWWNEHKILGVKYTYLDERPVPDWKDAKRQRADKWEVLEEALFEWAQRIFDAEPRIFGAVYPAIKANPALNGPKDGFKASKARHNEASSADINDRTRDIMGDTRKASQSFDAEDIFNMDETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6H0</accession>
    <name>A0A8H6C6H0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">EXPERA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_006663</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146996.1">
        <property type="nucleotide sequence ID" value="XM_037297560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6H0"/>
    <dbReference type="GeneID" id="59335064"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000247">
        <property type="term" value="F:C-8 sterol isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047750">
        <property type="term" value="F:cholestenol delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004769">
        <property type="term" value="F:steroid Delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007905">
        <property type="entry name" value="EBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207:SF0">
        <property type="entry name" value="3-BETA-HYDROXYSTEROID-DELTA(8),DELTA(7)-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207">
        <property type="entry name" value="STEROL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="7" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="15">
        <location>
            <begin position="67"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6217561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="244" mass="27778" checksum="8D93F307719FC02A" modified="2022-01-19" version="1">MADYILNSTSLPQAIHPFYPLEANIVGYLANRWSVPTLLGIFAGGWVVILGITLALVRSHNPNLPSRDKATILWFVLTGTIHLFFEGYFAFNHANMPGKQDLFGQLWKEYSFSDSRYLTSESFVLCMETVTAVCWGPMSFAVALLITYEHPLRHPLQALVSLGQIYGDILYYATSMFDHYGKGLTYCRPEAYYFWGYYFFMNFIWIVIPGILLYSSISESAEAFKVLDQQTRKAQGNGHLKKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6U2</accession>
    <name>A0A8H6C6U2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dipeptidyl-peptidase V</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_006568</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147176.1">
        <property type="nucleotide sequence ID" value="XM_037297466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6U2"/>
    <dbReference type="GeneID" id="59334969"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF13">
        <property type="entry name" value="DIPEPTIDYL-PEPTIDASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="6">
        <location>
            <begin position="435"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="501"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="533"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6217741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="554" mass="61259" checksum="086F76B81AC6237C" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C787</accession>
    <name>A0A8H6C787_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147668.1">
        <property type="nucleotide sequence ID" value="XM_037297094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C787"/>
    <dbReference type="GeneID" id="59334596"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="41741" checksum="068977990818D580" modified="2022-01-19" version="1">MPSLLEIPPELVEQIISDLAEGEPPSRKLIYEQPSKSLLDHDYHPLKDLSCTCRILRRLCFTSLFSALKLNMEVGTGFLEFIKSSQLAGRVDSLVLYTTTGCLGNSSFLWPRMVEVVDRVNAPSMTIIFPPSLFTEILPYSLNLDHAWAFSIDYQVLHLEMAPNSVAPKTPEDVVKESNVFAMRPWTHCTYNEGSSVEAYSTYEYYLKDKPSLFCPSNSNQFVRTMQNSLDHITSLDLITVFPTNHLWSFGKFISFMKSLRHLRTQLAPTPSNNILDDPSSLGKCQTKDLWMEFESSYARLVDFVAGGGYDEFELGALDQFTILDYANAALRETIDGVVGNRLDEWERDRSGGRWTRRREDQKAISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C799</accession>
    <name>A0A8H6C799_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147605.1">
        <property type="nucleotide sequence ID" value="XM_037297031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C799"/>
    <dbReference type="GeneID" id="59334533"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="281" mass="31485" checksum="60B079DE2024E096" modified="2022-01-19" version="1">MFDQHLGMASPSKPRLRSAHKEKIVSEGYRDNDERELEKKAEGLPGSIPDNLFLHKESKSTLQDMKARHEQSMMNHKLLDEEYVKWFVEALEEDLDEEEMCMRQATGSISLAKLKAQGYGDEYTIERGHFNQFKKIWLHIESLSSVEYEVQNAELTVKAGGTLLLYSRKQHQNNSRVSMTLANVSLDSLKPGERRGEVMKVVAPFGLEGSTEGSLVERVSSSGVRCLKCLKGGEENGHDRYGTVGTIDGTGGRLSSGGVEVQYLRAKDGSLKKETLMAWKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7Q5</accession>
    <name>A0A8H6C7Q5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peroxin/Ferlin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005867</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147953.1">
        <property type="nucleotide sequence ID" value="XM_037296772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7Q5"/>
    <dbReference type="GeneID" id="59334272"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peroxin/Ferlin" evidence="2">
        <location>
            <begin position="209"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6218518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="517" mass="58455" checksum="49BA935478CF16BD" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8H9</accession>
    <name>A0A8H6C8H9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_005440</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148332.1">
        <property type="nucleotide sequence ID" value="XM_037296350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8H9"/>
    <dbReference type="GeneID" id="59333846"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11062">
        <property type="entry name" value="CYP58-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF156">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6218897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="54640" checksum="E9AB6A609B925ABF" modified="2022-01-19" version="1">MFASVPVLIALTLVPIVYRVRFHPLSHVPGPIIAKVSSLFLHFICYLGIEGRVLRYYHQKYKTKVLRVAPNSVSISDSEAIRDIYIAEGGFSKDDRYRNFNLGPIVSIFSAIDTAYRDVRAKAVAPLFSPAQMRTESGSGGVIGSCVAEFVNQLCEFKTARIKTDILDLCARLSIDVVTAYLLGQRYGGLDENKHRTLKERQLDDAKLSANPFIFAIVAFARFSLLPNSIFRFVYAMSQRLSANEQVIESFVKLDHFAEEVMGSTVSATKPHYYHERLVQAGISHAEAAAQSKAIIFAGADSTAVMLATILFHLVQSESARVRLLCEIRSARHQDSGLPFLRAVVKEGLRLGMANPTRLTRVVPASSPGLRVGDILIPPGSIVGCAAYNLHHDPDVFPDPFTFQPERWLDDGTDHGLRRSGMERSMIPFGAGLRACIGKNLAMNQLHETVMAVVESEVLEGARTCQEKIEVIEWFNGDIKGHRINIEWRPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C925</accession>
    <name>A0A8H6C925_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">S-adenosyl-L-methionine-dependent N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_005447</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148339.1">
        <property type="nucleotide sequence ID" value="XM_037296357.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59333853"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049492">
        <property type="entry name" value="BD-FAE-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041068">
        <property type="entry name" value="HTH_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF21">
        <property type="entry name" value="NON-REDUCING POLYKETIDE SYNTHASE AUSA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20434">
        <property type="entry name" value="BD-FAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18558">
        <property type="entry name" value="HTH_51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="10">
        <location>
            <begin position="366"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="11">
        <location>
            <begin position="1257"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="9">
        <location>
            <begin position="1652"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="7">
        <location>
            <begin position="1257"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="7">
        <location>
            <begin position="1418"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1599"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1742"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1615"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1760"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="7">
        <location>
            <position position="1291"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="7">
        <location>
            <position position="1479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6218904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2535" mass="276821" checksum="DE3C87D55E0B4682" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C938</accession>
    <name>A0A8H6C938_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148673.1">
        <property type="nucleotide sequence ID" value="XM_037295975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C938"/>
    <dbReference type="GeneID" id="59333469"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000027">
        <property type="entry name" value="Protein FMP25, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053245">
        <property type="entry name" value="MitoProcess-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47563">
        <property type="entry name" value="PROTEIN FMP25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47563:SF1">
        <property type="entry name" value="PROTEIN FMP25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="281"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="345"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="595" mass="64571" checksum="20C3987F8880D4B0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9F2</accession>
    <name>A0A8H6C9F2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Iron-sulfur clusters transporter ATM1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Iron-sulfur clusters transporter atm1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_005109</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148719.1">
        <property type="nucleotide sequence ID" value="XM_037296021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9F2"/>
    <dbReference type="GeneID" id="59333515"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140466">
        <property type="term" value="P:iron-sulfur cluster export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03253">
        <property type="entry name" value="ABCC_ATM1_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000186">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="134"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="456"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="45"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="696"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6219284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="717" mass="79433" checksum="D72CA82E4DFDAFC0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CA53</accession>
    <name>A0A8H6CA53_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="23">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">HO133_004017</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="23"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148983.1">
        <property type="nucleotide sequence ID" value="XM_037294936.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59332426"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070867">
        <property type="term" value="C:mating projection tip membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990531">
        <property type="term" value="C:phospholipid-translocating ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140351">
        <property type="term" value="F:glycosylceramide flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099040">
        <property type="term" value="P:ceramide translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000048">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000108">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF180">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10 22 23" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 21 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 23" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="530"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="575"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1146"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1177"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1228"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1269"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1294"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1334"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="25">
        <location>
            <begin position="103"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="26">
        <location>
            <begin position="1114"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="23"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="236"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1395"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1479"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="1412"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1442"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="20">
        <location>
            <position position="644"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="644"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="644"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="645"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="646"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="646"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="778"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="819"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="842"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="877"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="957"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="958"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="959"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1062"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1068"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1088"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1091"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1092"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1092"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KAF6219548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1499" mass="169103" checksum="16667C46B0DBDCBF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAB6</accession>
    <name>A0A8H6CAB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_004122</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149088.1">
        <property type="nucleotide sequence ID" value="XM_037295041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAB6"/>
    <dbReference type="GeneID" id="59332531"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF8">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="75"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="288" mass="31444" checksum="80475B0199158BE6" modified="2022-01-19" version="1">MSLLPQTRSSPQRSALHPYPLSTSIPAKPGTNHSPQRPLPAELSIIPITVSTLPAYRRLITLLLPIRYADKFYKDSIANPTPSSLALCALWNETSRPGKRKRDSLNDELPSSVPAAGGPEAKVIGGIQCRLEPLPCPPPITPPTTSTAQPPPPQQQSHNLYIQTLAILSPYRSLGLATALLDAIISTALTHYPRQNINVREIYAHVWEANEEALEWYVKRGFEVGEEVVKEYYRKLKPSGARVVRRRVGVGDWVGVAGVWEGVVIGRGGGKEEDGDRGVMGKEGFGHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAR0</accession>
    <name>A0A8H6CAR0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149166.1">
        <property type="nucleotide sequence ID" value="XM_037294478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAR0"/>
    <dbReference type="GeneID" id="59331967"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="17"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="538" mass="61404" checksum="572D63E4373D2141" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAT1</accession>
    <name>A0A8H6CAT1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149469.1">
        <property type="nucleotide sequence ID" value="XM_037294781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAT1"/>
    <dbReference type="GeneID" id="59332270"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034970470" evidence="1">
        <location>
            <begin position="23"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="341" mass="36580" checksum="38A9EEB803E05980" modified="2022-01-19" version="1">MANYQSFVSALVFRACLQQVQAGIMGPPNFLEQPRDLSSFTCRAECSKSSTGQAILGHPGTSFCPYVCENGVQSLRLNTEMIQPASSHDPYFSNRKGNFQSKHTSYEHILATGGQVSNGAIAVSSINNTDGVGAGKDSYTQYCGDGSEFPPRSAWVSFENMFNDNKQIMFSSCSQYSQANDDGPEVGSIYNAIQQVAVETKVDHRFILAIIMQESGGCVRVPTSNYGVRNPGLMQDHNGNATCNSDITGVVQNPCPQDVVTRMVREGSAGTNDGDGLAQCINESDKDDVTAFYIATRLYNSGAVDGSGDLCKGIATHCYSSDIANRLTGWVEAPRGCSLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB46</accession>
    <name>A0A8H6CB46_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149523.1">
        <property type="nucleotide sequence ID" value="XM_037294142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB46"/>
    <dbReference type="GeneID" id="59331630"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="257" mass="29293" checksum="335FCBB430D596E1" modified="2022-01-19" version="1">MVSKEHRNGLRLRHRYQPEGHSPSAVRRLRLEWKLQADPNSGSYLSIVDRQAPIKPDIFISATHGYDNHQDELHHYWHACNERENSLEEPAFEDENTQFNANTSILQREQVPYSQCNFGVLSTALEFINRLPPTTHNCMTGFDTPVHNLVENQDEDNETRKSRPRNFDPDASPGIGTKESPHLSDDDSDPTYKDPFSTTVVPEQESRIDTDLDDVENGPRCASSADFTPQIRNQQQAPNGPNDRTRIRAHTNPPTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB64</accession>
    <name>A0A8H6CB64_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003249</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149553.1">
        <property type="nucleotide sequence ID" value="XM_037294172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB64"/>
    <dbReference type="GeneID" id="59331660"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="140" mass="15850" checksum="2AFC0406705F59DA" modified="2022-01-19" version="1">MENIDSNEPRTYNWNLLVVFSATDVQPRWRGPSFNKRPADRIHFRKGQETRVAIQRKDTDEGYNIDWKSATLVNVDVTTEAEKPDLKLTSRQKGRNLVLATVTAADGDTETGTDKTETWVVTVASGSGELYRIIKSLDTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB99</accession>
    <name>A0A8H6CB99_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149694.1">
        <property type="nucleotide sequence ID" value="XM_037294313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB99"/>
    <dbReference type="GeneID" id="59331802"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="363" mass="40139" checksum="81CA6C94FC7F2189" modified="2022-01-19" version="1">MRRVKLEHQTDSLGRRDSLATRFLTWTRTEFILCANQEDETLQDTSPEVLTVTLNWLATVMGSIHVILGIVVFAGLLFISLLDASIVAVRYMVSILVCRLVLKFEISGARGTTKLEKREDVFPGLELQRRKPWAGTTTSFDSQKMPIARMYAARRVDRDEASQKMPIARVYAARRVDTIKYILVGIEALGDLFHRLEDITGSSMQRQPRGILRGNILNRNEGVALLCEAISVYEKARMLCASIKQQGNIALGAVYMFEDGPRQRVRNESFAASVARTDLQESVERLSGDLPTFRSLAFCIGSVVIDGNSSSGGQFIDAGAALSRHLMPKLRAARSLGLGIRLPPVAVGSYTDGCEDTAGHIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBA9</accession>
    <name>A0A8H6CBA9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149720.1">
        <property type="nucleotide sequence ID" value="XM_037294339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBA9"/>
    <dbReference type="GeneID" id="59331828"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="693" mass="76476" checksum="FCC0F35B340E2EF1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBE2</accession>
    <name>A0A8H6CBE2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA methyltransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TRM5</name>
        <name evidence="12" type="ORF">HO133_003370</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="10"/>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBE2"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="11">
        <location>
            <begin position="140"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="269"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="297"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6220238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="471" mass="53808" checksum="6B6D60902F22757A" modified="2022-01-19" version="1">MNESPESAMFRPPINRAMRALDRSFFKKSVPLSAARILDNKQISKYRTELGHDLLRLDRMPAVRSVRDSDGQEAKALLLRPEVKPDDASTWSPKLSDLVKASQVSLSPYNLELDYDYWNYHDIMSAILPEAEQDELPTGFSIVGHVAHLNLRDQYLPYKHLIATVLLDKNPTIRTVINKTDEVGEQNEYRTFSYELLAGDPDMNVEIHEENCRFRFDYSKVYWNSRLNTEHRRLVEKFQPGEAVCDVMAGVGPFAVPAGKKNVFVWANDLNPDSFASLDDAVRRNKVHRFVKVFNEDGRTFIRSSAERLLKTDTHVKIHKGVSRLKQPASTVKPIATLTSPKTFNHYILNLPASAITFLPSFIGLYVGREEFFTPHTDAKLPFIHVHCFSTKSDGNKEEEIKICKEVSEQIGYEMKPGSMETEGEVQVWDVRDVAPMKRMFCASFRLPGEVAFRVAENESRIQPLPSKERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBE5</accession>
    <name>A0A8H6CBE5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
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            <taxon>Pezizomycotina</taxon>
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            <taxon>OSLEUM clade</taxon>
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            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037294384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBE5"/>
    <dbReference type="GeneID" id="59331873"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="153" mass="17821" checksum="EF82414168EEBA27" modified="2022-01-19" version="1">MDDGKDAVQEQYPETSISQPPVEGRHMAKQRRHTYTENLTVIMAIMNVSEKAYRTLKTAKMQPQDHRQSRENEQRCRPCHQCMVPIGIQRWVGGSTRKRRRVHVGGQGESGIEHRRYEKNVAWLEWEPYDDATCGSPEDQQKVGVHEYEPQAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBJ7</accession>
    <name>A0A8H6CBJ7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
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            <taxon>Lecanoromycetidae</taxon>
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            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149733.1">
        <property type="nucleotide sequence ID" value="XM_037294352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBJ7"/>
    <dbReference type="GeneID" id="59331841"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="237" mass="26804" checksum="E28F9CB11A28DF8B" modified="2022-01-19" version="1">MAPAAATRSKKAAKRVTPASKVTKKTSNAANKANKAPKAKKAPKATKAKTPPPREDILVHDTSSGTMSPDQVDETPPPAAKVDLQELIAQLSKVQEERNNRHPHEGRVHKSRRHHRRHSRSHRHRHSDTSDSDSSDSKSDWEERRGVPFRKKPFLSIAERFRSVDVKYFKQIFFGTFKTKNFAKLAHMHTTPAKEDKDVNGHNHMMHCFHVYSVAAGRFAHVSVKEGLNEALEYRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC46</accession>
    <name>A0A8H6CC46_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003108</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150110.1">
        <property type="nucleotide sequence ID" value="XM_037294032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CC46"/>
    <dbReference type="GeneID" id="59331520"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="226"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="226"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="34"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="276"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="443"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="880"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="923" mass="102991" checksum="EEC06B7E4D260682" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCG3</accession>
    <name>A0A8H6CCG3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002648</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150402.1">
        <property type="nucleotide sequence ID" value="XM_037293573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCG3"/>
    <dbReference type="GeneID" id="59331060"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11387">
        <property type="entry name" value="bHLHzip_USF_MITF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051732">
        <property type="entry name" value="USF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117:SF3">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="356"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="334"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="404"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="508"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="223"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="404"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="422"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="508"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="546"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="574"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="583" mass="64144" checksum="5C6941B630AF4BFE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDA1</accession>
    <name>A0A8H6CDA1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_002000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150581.1">
        <property type="nucleotide sequence ID" value="XM_037292928.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59330414"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021838">
        <property type="entry name" value="DUF3431"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11913">
        <property type="entry name" value="DUF3431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="411" mass="46871" checksum="1DF5FEF81B830023" modified="2022-01-19" version="1">MSSLVPEHVLKQVQTFSLSSLVPEHVLKQIPGLPGYSDGHEYERLANGQHDEDSPQRATKYNYKIYASILCALLVTFLAFKGAVNTHISISTMPNTSSPTVAIPKYMPEEQINTTESQLQQINATKSNLDQIVDIPKETKALVVSARSQDVIHWMEDVQEDWIQYPYLVTEDTNPNSTLSVPANNGNEAMRYFSFIIDNYDSLPDIIAFRHGHNTTWHQRFDSAAEVNHLNLTTVHLRGYQNFNCELRVLGCDQHIYLADKQRAENATNDESNGRLSTRSDPPVDEAIYQNWDAWFGVPMPEDVTSACCAQFAVSKEAVYRRTKEKYMEYRQWLLSTNLESRYSGMVFERLWHVVFGMAPVKMLAGRAGTHNMQSIRDMFQVRSNGRRTSESFKPVLEVTAFEEQSRSRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDR7</accession>
    <name>A0A8H6CDR7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002416</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150995.1">
        <property type="nucleotide sequence ID" value="XM_037293342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDR7"/>
    <dbReference type="GeneID" id="59330829"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051694">
        <property type="entry name" value="Immunoregulatory_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549:SF33">
        <property type="entry name" value="MEMBRANE PROTEIN WSC4, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G09020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549">
        <property type="entry name" value="PAIRED IMMUNOGLOBULIN-LIKE TYPE 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6221560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="193" mass="20085" checksum="B06695D803620814" modified="2022-01-19" version="1">MSLDRTLSGGETNSDPSDSYEAPPSGDNAVSSTESTDTASFTQASAAASTSSSSKLASGHSSPLAPVTATVTALALLQLLRLHDIGDGAIAGIVVGIVIFVLLTTASVMLCWRRRRRFTQRAVSELPSHQTDAETKSDPSATIWIPELGQEAAVCGPRELAGTPKPLAAPLESHGVTEPYIAEGRLFKIGNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEG6</accession>
    <name>A0A8H6CEG6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_001925</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151392.1">
        <property type="nucleotide sequence ID" value="XM_037292853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEG6"/>
    <dbReference type="GeneID" id="59330339"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF2">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04150)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="406" mass="44247" checksum="756AFCB6E12CF4CE" modified="2022-01-19" version="1">MVGELVKKPEHEPRAFAVMPFVWSIGTIIGPAIGGTFARPAISMPSVFSPASIFAHFPYLLPNLLCAAILLISILFGYFFLIETHPDMQPWSTQEELDHTTAETPLMTAGATADYGVDLRADTYGTFNTVNINESKEWMLNADGTPRPASTSLKDKPKVFTRKIAMIVVALGIFTYHSMSYDHLLPIFLQDERVSTSSISQSPFNIPGGLGLAIRSVGTIMCINGIIALFIQAIIFPLFASWLGVWKTFVIVTVFHPVAYFIVPYLAFLPETSLYPGIYVCLAIRNFFSILGYPVLLILLKEAAPSRSVLGKVNGLAASAGAACRTIAPPIAGYLYGVGTQIGFTGLAWWGSGIVAIAGAFQILSIQRTKNKTATIRPMASFMRNAMPAEREEDVVHITVTDRDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEI4</accession>
    <name>A0A8H6CEI4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Capsule polysaccharide biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lcsJ thioesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151204.1">
        <property type="nucleotide sequence ID" value="XM_037292665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEI4"/>
    <dbReference type="GeneID" id="59330151"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051490">
        <property type="entry name" value="THEM6_lcsJ_thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475:SF4">
        <property type="entry name" value="PROTEIN THEM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41663" checksum="006C39A9AFD3D805" modified="2022-01-19" version="1">MPLSLETLPGKEVLSDTLVSSSIWKLLCIFLILVNIKTLPLVWHVRLLNAFRFVLRSERSRTAIKPAQLFQPLITESHGALMELDYNIHKSNSTYFQDVDIARTHLICTLFSAGIEQARRSKGQGILGGGNKTFGAALGAVCCSFRKEINPFERYEMWTRVLSWDRKWIYMVTHFVRKDAIKPKSFSMYPRQKNKNAGTRNVDKKEDAIIASALSKIVFKKGRLTIPPEVMLQASGLLPPRPADLALLQTEIYPVPTKRAMAQRVFDVGFRFFENFDLVWEATRNMLMPEPEEARYQWRDGSPDDIATKKNDQWTWDMVERERQRGLEMASKLGALEGLAGEFTAEADALGKHSDLWWFLGLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CER4</accession>
    <name>A0A8H6CER4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytoplasmic tRNA 2-thiolation protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NCS2</name>
        <name evidence="3" type="synonym">CTU2</name>
        <name evidence="4" type="ORF">HO133_001311</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTU2/NCS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CER4"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03054">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019407">
        <property type="entry name" value="CTU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882:SF14">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10288">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6222225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="499" mass="55028" checksum="C6EDF3A21873EACC" modified="2022-01-19" version="1">MSARWGKTDEHWAMRTDFLVGGSPKMEKELFMKFKPQWVPKLLRDVLSLSYVPGFDVAAAAQNPLLGKGNGAKRFDLSLTGDRYYLKILQIKSQPYALAPSRKLYHYLKSATSIIYNNEKFLKMPKPVAQNGQKKDSVSCARCHTAETALVVRTEHLCRECFMRYVATKVLKRLETNKLRGGFKDPQRTLLVPISFGVSSIALLHVLDQQLSNRAETGRHAGYNLHILFVEQSVVLGQPSFLESLDVLKQRYPSHTYTVISLEDCFDYSIGFDTSASADALTDGSIALDKRTCLKHILSSVPSATSRVDMIEILRRRLILAFAKAHACDSILFGDSTTRLAEKTLSETAKGRAIALPWLTADGSSSDGTNCTYPLRDLLRKELGSYVSMTSPPLTPLICEAPFQRPTSSKDTTIDGLMTQYFESVEHNYPSIVANVVRTAGRLVAPSTSKGTNACALCGYPIVNEAWGGDQLDSGVPQVADSSGSEYGNSFCYGCARTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CET2</accession>
    <name>A0A8H6CET2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Steroid 5-alpha reductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151311.1">
        <property type="nucleotide sequence ID" value="XM_037292772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CET2"/>
    <dbReference type="GeneID" id="59330258"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010721">
        <property type="entry name" value="UstE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251:SF15">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE (DUF1295)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06966">
        <property type="entry name" value="DUF1295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="29195" checksum="8CF9366023FA7549" modified="2022-01-19" version="1">MPREPKDWDNQSREKKPSPLGTSIFVGLRAADTVLQYSILQRGWGSQLIQSLGGSVVPFATPRDPALAYFGLGPYPAILSALALGASTKHVAWVLGVSEQEMLPAGALMIGVFNSVVNTLNTMFSLWTITSAAPQMATQSASITDVITSSPTLMLGLGLYTVGIVTEFASELDRKRFKDKPENKGKPYGGGLWSWATNINYGGYTLWRAGYAVSAGGLPWGAFVGALFAYDFASRAIPSLDKYCTEKYGDQWTDIKKRVPYRLLPFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF45</accession>
    <name>A0A8H6CF45_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001319</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151668.1">
        <property type="nucleotide sequence ID" value="XM_037292249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF45"/>
    <dbReference type="GeneID" id="59329735"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF1">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="53377" checksum="12E9474EAF9F2E6E" modified="2022-01-19" version="1">MKLKTTGSKAVVLTRRSPRTTNNFGFLSILGFTCTILITWEAVFIIFDVSILNVGPAGFIYGFLFVWAGTLATYASLGELASMAATAGGQYHWVSMLAPPGSQEILSYMTGWLTVIGWQASFATANFVSAALIQGLIVLTRPSYYPKPYQHMLLFWAVMAFAVFINVLASTELPKFEGFILVLHIVGYSAILLPLLILGEHQDPHQVFGLWLNLGNLPTQGTSFMVGLLGPVFMFLDADGAVHMSEEIQDAPTVVPWSILFSTVINGALALSMMIATLFVTVDLQSSPNSPTGYAFMDTYVQATGSTAGATAMACIITVMQLFANVGLLASCSRMTWSFARDRGLPGHRTLAKVNPRTSIPVATIIATTITSTLLSLILLGSSTAFNNIVSIAVAGLSASYVLAIGLLLWRRTTGGIRHSPLSGSQLTNASGFELSWGPWHTPGIKGPAVDLFAIIYVLIILFISFWPPDVPVDGANMNYTILVTGAVLIFSVTWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFE0</accession>
    <name>A0A8H6CFE0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151818.1">
        <property type="nucleotide sequence ID" value="XM_037292399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFE0"/>
    <dbReference type="GeneID" id="59329885"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="414" mass="44847" checksum="20FE6389F1D7E4BE" modified="2022-01-19" version="1">MPNSIPDIQGLVEEFIWLRYDSLISAKRAITPPRKIPALSFLMQPPAGDLSVRRRAGDRSSVLSIYSQPKDRKPSPPSTSPSHEPRPPSSSQQQQPDHVTPSPKPAQPSSQLTTATPPNLSVGPIPLNPRQHIPPPIALRATLTLSTGGILPLAGYFAPGTTLTRLHPPFIHYHPRPPSSGAASPYVLQRSRTVGAGAEESVRRAEPAREQRAPWTPRLETCAVGFRRGDRRSGAGASVRDQGADRCDVLVLPVCVRAVRHADPVAGYWVCGRPFRFSSVREGRRAEAGGVCVVGEDVVAVDGEQGGGVVFRARWNARDGPSAPFRTLLTRLDRLWGVQWGLGCGRGDRVIFGVERGAVGKSFPPLCGGDAVRIEELTALTGRFGPVNPSQLNNNHRNQNNQVYVHEMARRGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG03</accession>
    <name>A0A8H6CG03_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000878</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152173.1">
        <property type="nucleotide sequence ID" value="XM_037291811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG03"/>
    <dbReference type="GeneID" id="59329296"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000097">
        <property type="entry name" value="DnaJ homolog subfamily C member 7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000055">
        <property type="entry name" value="DnaJ subfamily C member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052758">
        <property type="entry name" value="SRC_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44200">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44200:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="156"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="343"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="389"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="427"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="515"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="636"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="664" mass="72836" checksum="92E7DD81D7DE857B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGM8</accession>
    <name>A0A8H6CGM8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adhesin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000877</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152172.1">
        <property type="nucleotide sequence ID" value="XM_037291810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGM8"/>
    <dbReference type="GeneID" id="59329295"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="54469" checksum="225EAC87DA4FF829" modified="2022-01-19" version="1">MGRQAIFLSDEKPEDVEGYPDYSKNGDKLRPSAKHFPIGSETEASASPIRVFVKWALICTLIVLAMTFTLELFEPQSSKARRSLRKTVYYGEIPKTCPGTKHRKTLSFDFNKPTELAFAQESHDLKRHGPDFGFVRTVGKVEVLKQDSLSNVRIALDIRSSDPQLTSSESLSVVKSGSALTVKTPRRTPRESSSAGGAELPCIYVAATVSIPPGTTLENLGIKTETLSVTFFPGLDYAITNRTEINAHSASLSLATDEPPSIVINSRETRIHLNSGSVTGSYPLYDTLDISTTSGSIDIDVEPKDAEEGNVQPAILRLSSQSGSIRALTSTVSVPARDYQMTVKSLSGSIDATLLHGSRTSMRSNNGRINADLYPYGHNDSRTDINTHSQSGSTDITVHSSLSHPIDPIRKLYSEHHGLSGSLNLWYPAQWQGTVKGSTLSGGIDLDWDGLKVVKDEKRGWVKRTIEAVRGEGESQLIFSGKSGRVNLGGDSGGGVFAERKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGP9</accession>
    <name>A0A8H6CGP9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_001112</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the citrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152406.1">
        <property type="nucleotide sequence ID" value="XM_037292044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGP9"/>
    <dbReference type="GeneID" id="59329530"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF4">
        <property type="entry name" value="CITRATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6223060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="391" mass="43330" checksum="1540C91BAAB3CFFA" modified="2022-01-19" version="1">MTQDMDYLHVLDSRTEKSYKIPIQDNFIQATDVGKITLPDASHEEKNTSVENARPLRLLDQGFENTACMVSSITVIDGHHGEFRFRGLPIEDLFRTYIYEDVMHLLIWEALPSPKQKEDVRTALCQAAIPPETVVNVISAFPQDSETYNMILAGMSAFASSDSIMGATRHQQKPMFQGNLEKADKAIVRTVSYMAATIALVYCHKRGKNFTNPQPHRSLIGNLLLMMGFIDAETSSDSKVEECLNKLWILYADSEITNSTAAALHAGSSLTDPVSCAIAGLVSGFGPLHGGAIELAYEGFEQIGKVENVGPFMEAVKAKKARLFGYGHRVLKKRDPRAALIKEVMPPEDSTNPLLQIAFEVDRAARKDPYFVERGLHANVDLYGSFPYLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH17</accession>
    <name>A0A8H6CH17_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_001073</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152367.1">
        <property type="nucleotide sequence ID" value="XM_037292005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH17"/>
    <dbReference type="GeneID" id="59329491"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04813">
        <property type="entry name" value="PA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000364">
        <property type="entry name" value="Protease-associated PA domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051653">
        <property type="entry name" value="E3_ligase_sorting_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47168:SF1">
        <property type="entry name" value="OS02G0798600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47168">
        <property type="entry name" value="RING ZINC FINGER DOMAIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="605"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="737"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="108"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="300"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="657"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="782"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="189"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="325"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="522"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="786"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="830"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="845"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6223021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="884" mass="93379" checksum="4543C46433C80985" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHI2</accession>
    <name>A0A8H6CHI2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000476</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152850.1">
        <property type="nucleotide sequence ID" value="XM_037291415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHI2"/>
    <dbReference type="GeneID" id="59328895"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="452"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="178"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="505"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="520"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="569"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="610"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6223633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="665" mass="76523" checksum="424A5F99D6068F46" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI95</accession>
    <name>A0A8H6CI95_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010517</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6223943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153003.1">
        <property type="nucleotide sequence ID" value="XM_037301369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI95"/>
    <dbReference type="GeneID" id="59338907"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000039">
        <property type="entry name" value="Neutral amino acid transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF668">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="62"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6223943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="438" mass="47763" checksum="F8590D301E7150B3" modified="2022-01-19" version="1">MATNDYKHGYGGDGLVNGNSSSQDYDEKARSDFEVVPSKSKGSMGKDDPFGDETNSEVKYRTMAWWQAGMIMIAETISLGILSLPSVLASLGIVPAVILIIGLGIIATYTGYVIGQFKLAYPHVHNMADAGEVMAGRIGREIFGGAQILFLVFVMGSHILTFSIMMNTVTSHGTCTIVFGIVGMMVCIVFSLPRTLKKVSYMAIASFISILSAVMITMIGVGVERPGSGKVDVTVQSDLSKGFEAVTNIIFAYAGDLGHVAFFSFISELHTPESYPKAVFLLQGVDISMYLIVAIVTYRYAGSDVASPALGSTSPLLRKIAYGIAIPTIVIAGVINGHVASKYVYVRLFRGTDRMSKKSWSSFGLWAVIVLVLWTIAWIIAEAIPVFNDLLSLISALFASWFTMFLSRGHHLWTRTIFVRDGHQQGRHELKRELFMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIX4</accession>
    <name>A0A8H6CIX4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010919</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153402.1">
        <property type="nucleotide sequence ID" value="XM_037301768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIX4"/>
    <dbReference type="GeneID" id="59339309"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="86" mass="9440" checksum="A7B560555C78EAAC" modified="2022-01-19" version="1">MSTSANSTVLLSEELYDNFRKAIHTPLLLWIFGVCSVLFAAGMVYLELRYPVWSHIYFARDKEDAGERNGDAIGSARGGKEGGGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ01</accession>
    <name>A0A8H6CJ01_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Endothelin-converting enzyme 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010976</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153459.1">
        <property type="nucleotide sequence ID" value="XM_037301825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ01"/>
    <dbReference type="GeneID" id="59339366"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF167">
        <property type="entry name" value="FI17812P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="11">
        <location>
            <begin position="121"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="10">
        <location>
            <begin position="606"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6224399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="805" mass="90265" checksum="F134218459F826C8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-06-18" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJT2</accession>
    <name>A0A8H6CJT2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009962</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153635.1">
        <property type="nucleotide sequence ID" value="XM_037300818.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59338354"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056693">
        <property type="entry name" value="DUF7791"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF5">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25053">
        <property type="entry name" value="DUF7791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="3">
        <location>
            <begin position="416"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7791" evidence="4">
        <location>
            <begin position="721"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1471"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1526"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1550"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1474"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1526"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1556"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1587"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1681"/>
            <end position="1700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1709" mass="192276" checksum="7D14088BE9BDA4C1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK95</accession>
    <name>A0A8H6CK95_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153929.1">
        <property type="nucleotide sequence ID" value="XM_037301112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK95"/>
    <dbReference type="GeneID" id="59338649"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034914292" evidence="2">
        <location>
            <begin position="22"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="214" mass="23121" checksum="4223B9768C5E76D5" modified="2022-01-19" version="1">MAHRKVCRPFVLLFLLLRADAIVTLAISPTNPVSLLNDTIIAPASTPLAGLISQNATSPSLSQNMIDYRIVGTPLVLRITETGQTLSQSSVNRVIDAAIRRIVGKINSGAGSKPIDGGKFWQSSSDIEVRLNAISDGQLTYFLLGDAIAGAWQYMNEPWSSFREIVFDIVLASKGWDYIGYGRFAKYGPFPPPPPSPSSSSLSRSTNPAPNDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKC0</accession>
    <name>A0A8H6CKC0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153974.1">
        <property type="nucleotide sequence ID" value="XM_037301157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKC0"/>
    <dbReference type="GeneID" id="59338694"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF16">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="101"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="568" mass="64228" checksum="1B1AEF6B5B82D953" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKJ3</accession>
    <name>A0A8H6CKJ3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_010143</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FKBP-type PPIase family. FKBP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153816.1">
        <property type="nucleotide sequence ID" value="XM_037300999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKJ3"/>
    <dbReference type="GeneID" id="59338535"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000050">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050689">
        <property type="entry name" value="FKBP-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516:SF447">
        <property type="entry name" value="FK506-BINDING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6224949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="13091" checksum="03851C59F7BF0279" modified="2022-01-19" version="1">MGVTRKIVTKGNGTDRPKKGDTVTIEYTGNLYDEAKGSKNDFRGKQFDSSKGRGPFKTEIGIGKVIKGWDEGVTEMSLGEKSILTISGDYAYGDRGFPGLIPPNASLVFEVELKAVNDKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLK4</accession>
    <name>A0A8H6CLK4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_009625</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154334.1">
        <property type="nucleotide sequence ID" value="XM_037300486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLK4"/>
    <dbReference type="GeneID" id="59338020"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="10">
        <location>
            <begin position="377"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6225625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="653" mass="72599" checksum="F98F700AA0A2424D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM20</accession>
    <name>A0A8H6CM20_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ZZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_009854</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154561.1">
        <property type="nucleotide sequence ID" value="XM_037300713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM20"/>
    <dbReference type="GeneID" id="59338249"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02340">
        <property type="entry name" value="ZZ_NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054471">
        <property type="entry name" value="GPIID_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF15">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22939">
        <property type="entry name" value="WHD_GPIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="734"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="820"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="919"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="988"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="6">
        <location>
            <begin position="1062"/>
            <end position="1123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6225852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1162" mass="130610" checksum="5D77F24109861A38" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM89</accession>
    <name>A0A8H6CM89_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154558.1">
        <property type="nucleotide sequence ID" value="XM_037300710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM89"/>
    <dbReference type="GeneID" id="59338246"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="127" mass="13968" checksum="03EA3AC7631B1629" modified="2022-01-19" version="1">MTKYSTKPHSTMQDELRQLQEAEGEQHADRKKTGTSKNSKAAPPAAAGHYLKRTWSTGNEPSGEREPKHALVSNNSSTVDRWLKESMREQPWNNIGAILEPPPTKEAPGMRGETGSNSAVAGSEERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMM3</accession>
    <name>A0A8H6CMM3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Succinate dehydrogenase [ubiquinone] cytochrome b small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_009321</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CybS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155008.1">
        <property type="nucleotide sequence ID" value="XM_037300182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMM3"/>
    <dbReference type="GeneID" id="59337716"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03496">
        <property type="entry name" value="SQR_TypeC_CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.10:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] cytochrome b small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007992">
        <property type="entry name" value="CybS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] CYTOCHROME B SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05328">
        <property type="entry name" value="CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <note>ligand shared with SDHC</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <note>ligand shared with IP/SDHB</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6226455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="207" mass="22369" checksum="E368F494D88BA817" modified="2022-01-19" version="1">MASIVRPSLLRQSGVAPAAGRDLRKRAPQPYALSKLNSSSFRTQSIASRILPLRSDVLRGIPPSMLRVAGFHASGRRPILPAGPQIIDGTANDPAPIPQPSPTHGSYHWAFERLISAALVPLTVAPFAAGTLNPVTDAILCATILIHSHIGFQSVIIDYIPSKRLPKTRALFWWGLRAATLTVAVGLYEFETNDVGLTEAVKRIWIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMR9</accession>
    <name>A0A8H6CMR9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GIT Spa2 homology (SHD) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_009184</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154871.1">
        <property type="nucleotide sequence ID" value="XM_037300045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMR9"/>
    <dbReference type="GeneID" id="59337579"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005826">
        <property type="term" value="C:actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902716">
        <property type="term" value="C:cell cortex of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022018">
        <property type="entry name" value="GIT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013724">
        <property type="entry name" value="GIT_SHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039892">
        <property type="entry name" value="Spa2/Sph1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056439">
        <property type="entry name" value="VBS_C3G9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601:SF0">
        <property type="entry name" value="PROTEIN SPA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601">
        <property type="entry name" value="SPA2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12205">
        <property type="entry name" value="GIT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08518">
        <property type="entry name" value="GIT_SHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23742">
        <property type="entry name" value="VBS_C3G9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00555">
        <property type="entry name" value="GIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="4">
        <location>
            <begin position="126"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="4">
        <location>
            <begin position="183"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="222"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="696"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="472"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6226318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="873" mass="97044" checksum="F51ED1799B9D77DA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMT8</accession>
    <name>A0A8H6CMT8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trafficking protein particle complex subunit BET3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009263</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Golgi apparatus</location>
            <location evidence="1 8">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154950.1">
        <property type="nucleotide sequence ID" value="XM_037300124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMT8"/>
    <dbReference type="GeneID" id="59337658"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14942">
        <property type="entry name" value="TRAPPC3_bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000001">
        <property type="entry name" value="Trafficking protein particle complex subunit BET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016721">
        <property type="entry name" value="Bet3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13048">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018293">
        <property type="entry name" value="TRAPP_I_complex_Bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6226397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="200" mass="22780" checksum="04ADB1D6B93F1057" modified="2022-01-19" version="1">MASTKAARVGEEVWNLDESLTLRRTRVDKVNAELVTLTYGTMVAQLCSDFDSNYAEVNKQLDKMGYNIGMRLVEDFFAKSGTQRCANLRETAETISKVGFKIFLNITPTVTNWTADNKQFSLIFEENPLADFVELPDDGRAQDELWYSNILCGILRGALEMVQMQVEAHYVSDVLRGNDITEMRITLLRYVEDEMPPDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNV7</accession>
    <name>A0A8H6CNV7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155134.1">
        <property type="nucleotide sequence ID" value="XM_037299133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNV7"/>
    <dbReference type="GeneID" id="59336663"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6226825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="205" mass="21874" checksum="08F32D61E89AF110" modified="2022-01-19" version="1">MLVVVGLDVCSTALDFEVLVVVDLVELDGVNLEKDEADDAATNTCVLAVVAWVVLFAALGLELVGTRELETVVTVFALDETKVMEAEVEIAGAKEETVRDEANATAALVLVGEDFATNKGCRVSKNDLKDDAVEEERLLPDGLENPIEEAPEVLDAVLEEKTLEVKLELDCARAGLAIAAKKTMMAKILMTAKANVSDALGLRAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPF4</accession>
    <name>A0A8H6CPF4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155530.1">
        <property type="nucleotide sequence ID" value="XM_037299529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPF4"/>
    <dbReference type="GeneID" id="59337059"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6227222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22537" checksum="E9C33A9B175C6148" modified="2022-01-19" version="1">MAELELRGKGWRDGTGLGKRLNRLKHATVAFRPLPHSASQPPEDGLETQIAQPPSHRLRPNITPILRQTITGTQHPRPHNPNIRQLHIAKPTVPSTADVGSEGESVSENPKVDSSDRKSIPAIWKPVIDASSAWFHDGERRWRRGLRYSNGEMPRDADGVNRAVQAWTRKKQSADAANLPETVKKACKRKSAFLRNLEHDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPL1</accession>
    <name>A0A8H6CPL1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008798</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155662.1">
        <property type="nucleotide sequence ID" value="XM_037299661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPL1"/>
    <dbReference type="GeneID" id="59337193"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF6">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="502"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="582" mass="64619" checksum="D0F253CA30ED29A3" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQS8</accession>
    <name>A0A8H6CQS8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007606</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155812.1">
        <property type="nucleotide sequence ID" value="XM_037298476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQS8"/>
    <dbReference type="GeneID" id="59336003"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="54965" checksum="ED3181EE769A9BDE" modified="2022-01-19" version="1">MVPTKTNQTGTPEDSVTRPNINHLPSELLHMICVYLEPRDIATIRILDRIIAEVGLQYLVSQIHLIPKPDSFDRLLAVAEHPLASRYVNSLFYEADLLRSFRHPEEDRELWEKSLVGPSHASPLEEVQDPHFASACGHLPKRYNRGTSAVVLSHQQHYTKRELQRAYQTYRSYRAEQHRLNESAAYEEAMVRAMKRLPHLTSIFISCERGNTNHFRAAFEAGLCEDVTSDPRMADQICASQLSLLLSAADKAGLQIKKLICGSLGQIFLSCSLERLDAMQRSIQSLHNLHLFFSHIPLEFDSNAASNPHGSSSARADNMQFAMSAPNLETLSIRFDKDRALDPPDLKHLVSDFHWSYLASATFAKMSTGSEILVGFCDRHAGTLKDLSLTDISLYSGRWATTFYEMRQRLKLDKMALAGIIQSVEGDYWNFEIGDHTTKLMIERYIAYSDETQRDLSLGRFIMDFRQALAKRWAPHRPSGLSAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CS09</accession>
    <name>A0A8H6CS09_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNL-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008026</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156230.1">
        <property type="nucleotide sequence ID" value="XM_037298894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CS09"/>
    <dbReference type="GeneID" id="59336423"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024158">
        <property type="entry name" value="Mt_import_TIM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007853">
        <property type="entry name" value="Znf_DNL-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922:SF13">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05180">
        <property type="entry name" value="zf-DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51501">
        <property type="entry name" value="ZF_DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNL-type" evidence="6">
        <location>
            <begin position="95"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="65"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6228296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="194" mass="21729" checksum="E9C97A012A2AC071" modified="2022-01-19" version="1">MMKADQILSIIFRQLRPSLHQPHSANSHLRPLSQFAPLRHPSFPRPSCTPKHRIAQPSFALRNCQTRRVSDSTSAPSSAVAHSPNYPSNTQSPLEKPPAYELTFTCKPCKHRSTHTISKQGYHKGTVLITCPECSNRHVISDHLKIFGDQPFTIEDLMRQKGQLVKRGTLGGKGDLEFWDDGSQTGKDTLEGHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CSS1</accession>
    <name>A0A8H6CSS1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156556.1">
        <property type="nucleotide sequence ID" value="XM_037297921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CSS1"/>
    <dbReference type="GeneID" id="59335426"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="282" mass="29520" checksum="2A7FD4FF35F4A766" modified="2022-01-19" version="1">MKTKDADFVGDTRWGEAPPAKEQAAAIPAKKKTPVQEKKVLSSAATALQTAIAPVDQPQSPTAKQSQLTPKEPQQPTTETRPSTPSGQPGAALGGPAGRAPKRTSPKSTTLPSAPVQKARSDRPPRYSPDLLGSSPSPPSTPASPAGSGLDESVTRQSHQRRKSPDLPVLAPAPSPTSGFGKYSVPDLFQDEDPSFDLPASGTSSPASSSFPPSSGSVETPTRKPAGDQTGKPPPSKMPKLTLKGPKSKDKTEHPSLPLPATFDSPLRDFYSRLVTMKVILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7A1</accession>
    <name>A0A8H6F7A1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147345.1">
        <property type="nucleotide sequence ID" value="XM_037297220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7A1"/>
    <dbReference type="GeneID" id="59334723"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="125" mass="13111" checksum="7118FA4B1BC4D457" modified="2022-01-19" version="1">MALQTIYLATDSSSGQQGTYIAFFSDSDPCNDGTRFGFLPQGFNNCNQNLTILGHPDITFTGCKPYTGYPGSLPTGVSDGGTPALKCVPATNPPSATSGPNSEYGVYEICNTNNGGFVDVIEYCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F823</accession>
    <name>A0A8H6F823_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147856.1">
        <property type="nucleotide sequence ID" value="XM_037296675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F823"/>
    <dbReference type="GeneID" id="59334175"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="129" mass="14433" checksum="AD334CB973921840" modified="2022-01-19" version="1">MPPQMPDCPRQSPASIDPATDMCRKWTLWGRGDYLDSVICCKVFPERHSMDDGDIARALSILHKQCANMTGPDAAELWRFLETHPNLHTNGLVGWRKIKDGGPMDKVEEAKVTQAINRFKNQVPSAGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8M7</accession>
    <name>A0A8H6F8M7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Flap endonuclease 1</fullName>
            <shortName evidence="15">FEN-1</shortName>
            <ecNumber evidence="15">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HO133_006040</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleoplasm</location>
        </subcellularLocation>
        <text evidence="15">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="15"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148124.1">
        <property type="nucleotide sequence ID" value="XM_037296943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8M7"/>
    <dbReference type="GeneID" id="59334445"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09907">
        <property type="entry name" value="H3TH_FEN1-Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000003">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="18">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="17">
        <location>
            <begin position="146"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="357"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="357"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF6218689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="396" mass="44657" checksum="FD2087CE7ECB4249" modified="2022-01-19" version="1">MGIKQLASIIHEHAPDAIKQGEIKNHFGRKVAIDASMSIYSFLIAVRSEGQQLMNENGETTSHLMGMFYRTLRIVDNGIKPLYVFDGAPPKLKSGELAKRFARKTEATEAHEEAKETGTAEEVEKFSRRTVRVTREHNDECKRLLTLMGVPYIVAPTEAEAQCAVLARAGKVYAAASEDMDTLCFDSPILLRHLTFSEQRKEPILEIHLDRVLAGLAMDRKQFVDLCILLGCDYVDPVPKVGPSTALNLIREHGTLENVVDFIKNDPKGKKFTLPDDWPFEDARDLFFSPDVRAADHPDCDFKWNAPDVEGLVKFLVTEKGFSEDRVRSAATKLTKNLKTSQQARLEGFFKPVQKTEEEVASLKRKNEDKREATKKKQKLEKKEKKEAKSKPRGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8N5</accession>
    <name>A0A8H6F8N5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">rRNA adenine N(6)-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_005100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148710.1">
        <property type="nucleotide sequence ID" value="XM_037296012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8N5"/>
    <dbReference type="GeneID" id="59333506"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034246">
        <property type="term" value="F:mitochondrial transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006391">
        <property type="term" value="P:transcription initiation at mitochondrial promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF17">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="65882" checksum="2DD0CDB6FE36F0E5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9B7</accession>
    <name>A0A8H6F9B7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149221.1">
        <property type="nucleotide sequence ID" value="XM_037294533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9B7"/>
    <dbReference type="GeneID" id="59332022"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="288" mass="33859" checksum="547F38B95FEF5FD1" modified="2022-01-19" version="1">MGACGPPESAEFMLSRDFLPFGASEPTNDVITYPPLAMALRLYAVAYLAEYKYEVLHDAEVWHDAEKEQWESLIIRLLRTGANLHAPVPHFVEMRNESDVSYEVTKYGTPLDEMFTFTDTPAEARAVANGWLQILRSEGYDVVAYIEKESDLHYTRSQTTFPLLQRHEEHDSPRELIFELEQEKQSIYWDWWTDPSSSIYLLRSTLKPMLMLSRSFITYHMSSEVTWPFHYPKWYGYCHPTREDEEAVQQQLYDMAQRRANRRLQKRHAKESRSRGLKYPSMPGAWQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9C2</accession>
    <name>A0A8H6F9C2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein SLA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HO133_003233</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SLA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149537.1">
        <property type="nucleotide sequence ID" value="XM_037294156.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331644"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11773">
        <property type="entry name" value="SH3_Sla1p_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11774">
        <property type="entry name" value="SH3_Sla1p_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000256">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.700">
        <property type="entry name" value="SLA1 homology domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013182">
        <property type="entry name" value="DUF1720"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056996">
        <property type="entry name" value="PH_SLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007131">
        <property type="entry name" value="SHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035800">
        <property type="entry name" value="Sla1_SH3_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF58">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04470)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08226">
        <property type="entry name" value="DUF1720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24081">
        <property type="entry name" value="PH_SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03983">
        <property type="entry name" value="SHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 18" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="11 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="22">
        <location>
            <begin position="42"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="21">
        <location>
            <begin position="457"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="21">
        <location>
            <begin position="525"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="21">
        <location>
            <begin position="828"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="583"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="920"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1016"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1129"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1486"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="586"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="605"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="658"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="920"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="950"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1016"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="1137"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1174"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1201"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1225"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1244"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1271"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1306"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1391"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1411"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1508"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="1558"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1570"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1609"/>
            <end position="1619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAF6220102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1619" mass="176390" checksum="D266C85A6B3CE863" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9D1</accession>
    <name>A0A8H6F9D1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6220010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149445.1">
        <property type="nucleotide sequence ID" value="XM_037294757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9D1"/>
    <dbReference type="GeneID" id="59332246"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="312" mass="35211" checksum="DD21F3C38CD42673" modified="2022-01-19" version="1">MESNYPYSQNANQPHSQQARDEHRPTHFQNGLSGQQAGFGLGPTPDTETYGYPVSQYHGSHTQQTRFQGSQEPQHTDFALHQPLYNPVTGIINDPFLSDTDFQYQETDYRTQYTGIGANPSWPHVGFQNQQTGSYTQYTGVGNNPPWPHVGLQAAQPAFASPHQEADFGHGFQFEPSTVALQYNQQQQADRNFQPYHPGAVASGHHQQAAGFGVPQQIQPSWDGTQAPQWQPGYTQQRVTVPAVDLQDLHLSQLRVLLEMHGAPLMFDYRDLNLDQLRKMMGKRGIRFRKDRCSKADLIQALQNYDRFKGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9E8</accession>
    <name>A0A8H6F9E8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149505.1">
        <property type="nucleotide sequence ID" value="XM_037294124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9E8"/>
    <dbReference type="GeneID" id="59331612"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="278" mass="32052" checksum="68BE6E13FA1989F0" modified="2022-01-19" version="1">MSTKDSGTIRQMEHLIEENFGLWFVNTRAELRSKKLWKYTQGEYESEDSAVTTATTSTDTEISPPITKKQEKAMKEWEEKSQEAADLMTPTISPGIQQKLTEAEFNDGYLMLTRLQTLLQPTGSSEFMRLSKEYYTLQFKAFKTVPEYLTHIKVLEEKIDATKVTLDTNNRTILCLSISLPREYQYLIQIWAVTPSITAEKARQMVLEASRQHNQALHSQDSARFFKAGFQGKGKEKEPCNHCGSTGHLSEKCWTEYPQLAPDWYKEMMKDQAKHKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9R9</accession>
    <name>A0A8H6F9R9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heat shock protein 60</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003773</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149383.1">
        <property type="nucleotide sequence ID" value="XM_037294695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9R9"/>
    <dbReference type="GeneID" id="59332184"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6219948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="62635" checksum="39FCA4434C79DE7E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9Y2</accession>
    <name>A0A8H6F9Y2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA repair protein Rad26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003058</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150060.1">
        <property type="nucleotide sequence ID" value="XM_037293982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9Y2"/>
    <dbReference type="GeneID" id="59331470"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048379">
        <property type="entry name" value="Rad26-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022093">
        <property type="entry name" value="Rad26-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048380">
        <property type="entry name" value="Rad26-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21046">
        <property type="entry name" value="Rad26-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12331">
        <property type="entry name" value="Rad26-like_helical_rpts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21048">
        <property type="entry name" value="Rad26-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad26-like N-terminal" evidence="5">
        <location>
            <begin position="418"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Rad26-like helical repeats" evidence="3">
        <location>
            <begin position="523"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="Rad26-like C-terminal" evidence="4">
        <location>
            <begin position="733"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="253"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="800" mass="89119" checksum="655A0053EEC02E98" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FA14</accession>
    <name>A0A8H6FA14_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_002908</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149911.1">
        <property type="nucleotide sequence ID" value="XM_037293833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FA14"/>
    <dbReference type="GeneID" id="59331320"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF9">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="764"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="826" mass="93821" checksum="F3C2178A8BED9F39" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAA1</accession>
    <name>A0A8H6FAA1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149938.1">
        <property type="nucleotide sequence ID" value="XM_037293860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAA1"/>
    <dbReference type="GeneID" id="59331348"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="472" mass="56388" checksum="7D92CB6E26D0C070" modified="2022-01-19" version="1">MPRHTIPAPGTRWPMRKAAMREMDMRVKNDQRGGRDPGTYNRPRTILRDGKNFELPPVLYGEKPITVRPDNPIKSLLRNDPESSDWIPDGLAQDPVYREFEHERAQWQRQGFTIPCKHVPDRKARYENEFMFWLHVHPNRERYKEELYPRRESPRYKRPEDRRNGGNDEYWHGKPPRLDGRGTHVRGQQRYPWQRYDHNQGRPSYRGNQTLGVRDPRNRPPLPTNEICEIPGDRMYEVHRERDRQPRMVRRVPIAGGLPPYFESELYDPHGDRVRHAPLRPHSRDHVRKPLRAHPWMYDEDEPRDEHRSASSHRYHWGREEGEGEREEEEEEEGEEEEEEEEEEEEEEEEEEGEEEEEEEEEEEEEGEAISQVSSESFMRTRLNPLVVYPVPPPYHRRPPKHPRRNARHGDDTDDEDEDEQFGRRGPYVGRSRDPYAIGRERRHFGLGPRSCEREDLEYALGGDEDDDGYDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAF2</accession>
    <name>A0A8H6FAF2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150600.1">
        <property type="nucleotide sequence ID" value="XM_037292947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAF2"/>
    <dbReference type="GeneID" id="59330433"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="139" mass="14785" checksum="1EEA35E2826A02CA" modified="2022-01-19" version="1">MTAIFARQTCSNPFSSHLASTSRHNVADEMEGVERCEAAEEGKMVGVEPCDENQMQGVECDEHANDDTRMEGVQCTDDGGRSKASQGVQSGSKGGCEMETEMQGLESGGGVDGVREGEGDVQMLGSGWTWHGMAQCCIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAH9</accession>
    <name>A0A8H6FAH9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Piwi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150178.1">
        <property type="nucleotide sequence ID" value="XM_037294100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAH9"/>
    <dbReference type="GeneID" id="59331588"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="2">
        <location>
            <begin position="709"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1078" mass="118122" checksum="E2E42A2FE46C7619" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB14</accession>
    <name>A0A8H6FB14_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-keto-steroid reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002367</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150946.1">
        <property type="nucleotide sequence ID" value="XM_037293293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB14"/>
    <dbReference type="GeneID" id="59330780"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000253">
        <property type="term" value="F:3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051593">
        <property type="entry name" value="Ergosterol_Biosynth_ERG27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647:SF1">
        <property type="entry name" value="3-KETO-STEROID REDUCTASE ERG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647">
        <property type="entry name" value="DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="272"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="52461" checksum="392C6AFC8275128C" modified="2022-01-19" version="1">MDETDDLRPQDNEQHSYVLITGANSGLGFAICCRLIDEFISSRPTFECLTLIITTRDSRKSGDTVKRLERYLERQSRELDAPLKHRISLQPEQVDLTSLRSVQTLSTRLLGNLPRLDTIICNAGYGAFTGIDWPIAIKETLTDFVTSLTYPSFKLSATGETTAAQTGSRELDIKAGNSPNDEPPLGQVFTSNVFGHYLLSHQLIPLLSASPDHGRIVFISSIECLASHFNSADIQGLASSKAYESSKRLTDIIVLTSTLPSMKPFVQRFLTPSPSTPSQSILLLDNEPSDSTRSSTPDTPPQRISLSDNEYRLPTNPEPKIYVSHPGICATSIITLSIIPFYLRILAFYIARWLGSPWHTVSAYKGACAPVWLALAEQDELEDIEQREGKGKWGSVCDRMGHERVVRTAVDGWGLGGRMGEGDMSNRIGSRRGFQEVTREDREGFEELGRECWREMEGLRVAWEEMLRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB21</accession>
    <name>A0A8H6FB21_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_001870</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151337.1">
        <property type="nucleotide sequence ID" value="XM_037292798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB21"/>
    <dbReference type="GeneID" id="59330284"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF26">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="22"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6221902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="612" mass="66505" checksum="7598E0733D189FB1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBA5</accession>
    <name>A0A8H6FBA5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151231.1">
        <property type="nucleotide sequence ID" value="XM_037292692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBA5"/>
    <dbReference type="GeneID" id="59330178"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6221796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="554" mass="62669" checksum="CFA1BBF97034B641" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCD8</accession>
    <name>A0A8H6FCD8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000741</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat mio family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152040.1">
        <property type="nucleotide sequence ID" value="XM_037291678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCD8"/>
    <dbReference type="GeneID" id="59329159"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037593">
        <property type="entry name" value="MIOS/Sea4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049092">
        <property type="entry name" value="MIOS_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031488">
        <property type="entry name" value="Zn_ribbon_mio"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453:SF9">
        <property type="entry name" value="GATOR COMPLEX PROTEIN MIOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453">
        <property type="entry name" value="WD40 DOMAIN-CONTAINING PROTEIN MIO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21719">
        <property type="entry name" value="MIOS_a-sol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21720">
        <property type="entry name" value="MIOS_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17034">
        <property type="entry name" value="zinc_ribbon_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="MIOS-like alpha-solenoid" evidence="6">
        <location>
            <begin position="515"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="GATOR2 complex protein MIO zinc-ribbon like" evidence="5">
        <location>
            <begin position="895"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="436"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="484"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1032" mass="115040" checksum="F86643C8841C610A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCN0</accession>
    <name>A0A8H6FCN0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152339.1">
        <property type="nucleotide sequence ID" value="XM_037291977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCN0"/>
    <dbReference type="GeneID" id="59329462"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="400" mass="45821" checksum="536F57F1676F2EE6" modified="2022-01-19" version="1">MSMSSSAPSKPVWAKPSKRARSGEPRLFAELESMVLDTFQDLHDPEDLTWLWVGGRHVSKHFRCEVERMFRTVFLPETILRCDLGLHLLCWDVFTPDRSVGYAGCYGDMSSVREYTTKTPSFTLDRFSDDQARAFFKCGKARKEMVFRRLHEKQLVGSPVFSIQVREVVNDTGLPNLYIDDEKEEISVDWKGMLDQLLGEEHVFNTILSQRLGWHLDPGEDTPNLLETHRKLARRSRFQRECRAKYRSNILEHYTHDEKPALRNLQKLRLAASGSGIAHGDNENDNTLVEYTQREILDELPNGAIIYSGNSNPGPKPGSIPRGISTDEDYCSLMNQWRGLPPPSAADLFSPRPLPDLSQGRRPMDPPQRSKRQHHPSHEKRAREETAEMLPRKRGRSAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FD04</accession>
    <name>A0A8H6FD04_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_000394</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152768.1">
        <property type="nucleotide sequence ID" value="XM_037291333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FD04"/>
    <dbReference type="GeneID" id="59328813"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048630">
        <property type="entry name" value="Sec8_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20652">
        <property type="entry name" value="Sec8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="6">
        <location>
            <begin position="114"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec8 middle helical bundle" evidence="7">
        <location>
            <begin position="370"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="842"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="855"/>
            <end position="865"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6223551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1096" mass="123613" checksum="7D9E07326A5A50F8" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDR5</accession>
    <name>A0A8H6FDR5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010908</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153391.1">
        <property type="nucleotide sequence ID" value="XM_037301757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDR5"/>
    <dbReference type="GeneID" id="59339298"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000012">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000014">
        <property type="entry name" value="Probable acyl-CoA dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000027">
        <property type="entry name" value="Putative acyl-CoA dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="124"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="236"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="550"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="472"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="647" mass="71068" checksum="CF060DA2684A2727" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDU6</accession>
    <name>A0A8H6FDU6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009913</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153587.1">
        <property type="nucleotide sequence ID" value="XM_037300770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDU6"/>
    <dbReference type="GeneID" id="59338306"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="212" mass="23747" checksum="846D15626A99E906" modified="2022-01-19" version="1">METCSLRFASYLTNCLCVHTPGNHASPQSYSTDACAKQQYILIVNCRILKTPHSPLDPWTYMGPKGRYEFSNYRNPSIKPNWLATFLLTCLGDIFDATATQGFRPDEPYPDGTYNCHLDYVSTSGSRGDFALGITKIEAPVWYMTFSDIVDDLEALWYAALWFEERAAGVPEMDFNVYQFDRQSSDARGSSFLASRGNFSFALIGEADVGNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE47</accession>
    <name>A0A8H6FE47_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_011085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153568.1">
        <property type="nucleotide sequence ID" value="XM_037301934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE47"/>
    <dbReference type="GeneID" id="59339475"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="403"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="589" mass="64917" checksum="72AFB9511E946C52" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEC2</accession>
    <name>A0A8H6FEC2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_010457</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Mitochondrial peptide exporter (TC 3.A.1.212) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154127.1">
        <property type="nucleotide sequence ID" value="XM_037301310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEC2"/>
    <dbReference type="GeneID" id="59338848"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18573">
        <property type="entry name" value="ABC_6TM_ABCB10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000095">
        <property type="entry name" value="ABC multidrug transporter Mdr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000218">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="188"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="508"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="84"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="749"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="753"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="772"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="792"/>
            <end position="816"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005580"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6225260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="816" mass="88454" checksum="A23A2FFF5E11E626" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FEJ1</accession>
    <name>A0A8H6FEJ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP synthase subunit K, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010153</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153825.1">
        <property type="nucleotide sequence ID" value="XM_037301008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FEJ1"/>
    <dbReference type="GeneID" id="59338545"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ATP19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28074">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT K, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28074:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT K, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11022">
        <property type="entry name" value="ATP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="7794" checksum="2F9172C07E302A0D" modified="2022-01-19" version="1">MVAQYTIFGRQVGSHVLAMLTLGTFAAGTTFAMSGKEKAKEQGPPINASSKDEEEFIQEFLKNNGGEQKAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFA6</accession>
    <name>A0A8H6FFA6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alcohol dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="9">1.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_009238</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:15938"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:15939"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.2" evidence="9"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154925.1">
        <property type="nucleotide sequence ID" value="XM_037300099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFA6"/>
    <dbReference type="GeneID" id="59337633"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008106">
        <property type="term" value="F:alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000158">
        <property type="entry name" value="Zinc-binding alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="13">
        <location>
            <begin position="34"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="193"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6226372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="359" mass="38521" checksum="C165D918EB7ACFC0" modified="2022-01-19" version="1">MSTSYKFQGWLGFDPSSVKGNMKWQGYEPKTWEETDVDIKITHCGICGSDLHTLRSGWAPTIYPCCVGHEVVGTAVKVGSKAERGIKVGDRVGVGAQSSSCLKPDCEECASGMENHCMNMVGTYNGKYDDGSKSYGGYSDYCRAPSHFVLKIPDAIPSEEAAPMLCGGITVFSPLKKNGCGPGKKVGIVGVGGLGHFGILYAKALGADSVTAISRSAAKKEDAMKIGAEKYIATNDDKDWATHHARSLDLIVSTVSSPNMPLEQYLMLLRTNGTFIQVGAPEDRLPAFNAFSLIAKGCKIGGSAIGAPHEIEEMLELTAKKGVKPWIQKRPMKDANEAVVDMDAGKARYRYVLVNEAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG71</accession>
    <name>A0A8H6FG71_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008561</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155428.1">
        <property type="nucleotide sequence ID" value="XM_037299427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG71"/>
    <dbReference type="GeneID" id="59336957"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034023471" evidence="3">
        <location>
            <begin position="18"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6227120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="363" mass="33427" checksum="BE80C415A79B4DE8" modified="2022-01-19" version="1">MKQTAIAAAVLAVGATAQNSTSTRTEYYNTCSLSSGGIVTATATQTYCGPCEAAGMTTFAGGSLTTYTTKFLQVCPTGLAEATYTVTEPCPSTGLARGSGYVPQGFTVTTVPCSVCEAGKSQYAQTTRGPALASALAAPAPAPTVPTAPAEPAPPTAAGPAAAPGAPAAAGAPAAAGAPAAAGAPAAGAPAAPAAAGAASPANAENAAAAPGAASPAEKGAASPAENGAASPAENGAAAPAESGAASPAENGAASPAENGAAAPAESGAASPAESGAASPAENGAASPAENGAAAPAGSSAGAPASYPLPSQAGQVALASGAPASNGNSTVPFKGAASLVSAIEISFFITALVTLITGFAFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG90</accession>
    <name>A0A8H6FG90_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_008427</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155294.1">
        <property type="nucleotide sequence ID" value="XM_037299293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG90"/>
    <dbReference type="GeneID" id="59336823"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35043">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35043:SF7">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034224741" evidence="3">
        <location>
            <begin position="21"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="487"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="522"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="561"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6226986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="610" mass="68291" checksum="DC13C452C0B7830B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG96</accession>
    <name>A0A8H6FG96_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008881</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155745.1">
        <property type="nucleotide sequence ID" value="XM_037299744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG96"/>
    <dbReference type="GeneID" id="59337276"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.395.10:FF:000008">
        <property type="entry name" value="Ulp1 protease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6227437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="219" mass="25103" checksum="3E9A8A21D93211AA" modified="2022-01-19" version="1">MKEDIDTLKNDWLTDNHYTATPKRTFVRRADSVYRYLEREYLNQYKNAHIVLLRPSMSFMLMQTPDPHTLKEALPNFSRTTHIFLPINDCRNPSIAEGGSHWSLLLVSVVDGVAFHYDSLNPANRNEAGLAARKIGQLLGRQLQFIDLADSPQQENSSDCGVFVCIQMRHLLLRKLLMAHAKEKVSMSMGGKSVDATQGRKEMLRIIEGFRKEGERRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FGP7</accession>
    <name>A0A8H6FGP7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008636</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155502.1">
        <property type="nucleotide sequence ID" value="XM_037299501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FGP7"/>
    <dbReference type="GeneID" id="59337031"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000063">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="2">
        <location>
            <begin position="59"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="265" mass="29062" checksum="2641169E8737CF76" modified="2022-01-19" version="1">MNTRWAAASTAARLKNPTVFREKAYIDAQWLESLSSNNYGDRLMPRNVRRPCKMECPRLAQHSSRSSEDMNDAIEVAQRAFSTFQYTAPKERARIVQKWSDLMIEHLKTLAEILMYENGRPIAGARQGIKSAAGFFDWFRCEAERSYNHTASGTTSGNRVVTIQQPVGVVGVLTAWNFPSATITRKVAGAIAAGCAIVLKAATETPYSALALAELGERAGVPKGMFNVVTTNELVQEVGTAITTRKDVKKTSFTGSTHVGKLLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHN8</accession>
    <name>A0A8H6FHN8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156450.1">
        <property type="nucleotide sequence ID" value="XM_037299114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHN8"/>
    <dbReference type="GeneID" id="59336643"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF2">
        <property type="entry name" value="SET-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="297" mass="33847" checksum="4205F2CD70847ED9" modified="2022-01-19" version="1">MYTIRSIPGKGQGMFANKDIKAGSRILADELLLSIAESKIDEGIGERISVSLHGLPPEQQQQFETLHCPDHPTWTPAVSRFLANCFEVEAPGSGIFLKASRINNSCFPNAFFSWNQNLHRLTVHAMVDIPIGDEITVCYIFPFYSLQIRRDLFREHYVFECDCPACHVETGIGRRGELRRRRMETLYMAIDKTNDGPSNNDQKELEMVLEFIRLAEDERLDGQFLSCIYSRAKKCYDDRGSEQLALEYAEMEVETNMRLLGDDHAVTQESARDLDELKTRLAMVQQTEENGENLGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI30</accession>
    <name>A0A8H6FI30_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156563.1">
        <property type="nucleotide sequence ID" value="XM_037297928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FI30"/>
    <dbReference type="GeneID" id="59335433"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034424428" evidence="1">
        <location>
            <begin position="22"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6228921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="320" mass="36337" checksum="DBF65839261895C9" modified="2022-01-19" version="1">MQDTWTHLLLVLGLAAPQVMASLMETAAITSPTPTTMVTMMSANPVANESLVEVFVYPGPTTSPTLSVIYDHKVPAPTPGSVGILNGIPTRILNDTIKETTPILADAEHSQLYQPWNTSYPGPIKDGDWAKYLQIWDPVWWSTNLDSLRQISCICSAEDYNTNPQAQVAAYMNFEYYSQRLDNIYNFDWECGPAPSHMNIGERITSAKGRAEHCWSMAGGDPDDHQSLPMHDWWCDRNEELCVKPWHDGQRSLKFHDHVRNYDRHGVHTWSEIQQVVRDVCEPVCTDKFRMRFDDFSHHNPSFVTTFWPAKPIDLTVVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FI80</accession>
    <name>A0A8H6FI80_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dicer-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">HO133_007111</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="17 18">Belongs to the helicase family. Dicer subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6228997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156639.1">
        <property type="nucleotide sequence ID" value="XM_037298004.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59335511"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050688">
        <property type="term" value="P:regulation of defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18034">
        <property type="entry name" value="DEXHc_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000015">
        <property type="entry name" value="Dicer-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001669">
        <property type="entry name" value="Dicer-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000628">
        <property type="entry name" value="Endoribonuclease Dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.380">
        <property type="entry name" value="Dicer dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038248">
        <property type="entry name" value="Dicer_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005034">
        <property type="entry name" value="Dicer_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056755">
        <property type="entry name" value="DSRM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF62">
        <property type="entry name" value="DICER-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03368">
        <property type="entry name" value="Dicer_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24995">
        <property type="entry name" value="DSRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51327">
        <property type="entry name" value="DICER_DSRBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0930">Antiviral protein</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 18" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="24">
        <location>
            <begin position="164"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="25">
        <location>
            <begin position="488"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Dicer dsRNA-binding fold" evidence="26">
        <location>
            <begin position="684"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="PAZ" evidence="23">
        <location>
            <begin position="926"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="22">
        <location>
            <begin position="1085"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="22">
        <location>
            <begin position="1284"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="21">
        <location>
            <begin position="1466"/>
            <end position="1538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="117"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KAF6228997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1603" mass="181676" checksum="1352A08EE322D2A6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIA7</accession>
    <name>A0A8H6FIA7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PQ loop repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037298033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIA7"/>
    <dbReference type="GeneID" id="59335540"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF11">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="271"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6229026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="33514" checksum="2E92B128776EFBB9" modified="2022-01-19" version="1">MGSYEIDQPVSGACVDLSSPNIFNFILSILILLGILVSYLPQHHRIISRRSSTGISPYFVLLGTTSGTCAFANILTLPASRRDLACCRELSGFECVAGALGIAQVGVQWSCFMVILFLFLLFFPRTPSDAKSKDEPTYRLAITVFLTSIVHLVLTFLVSVIIIYLYRSRLASWAHFLGIFGTCLAAIQFLPQIWTTWKLQEMGSLSIPMMCIQTPGSFVWVGSLAARFGWEGWSTWGIYLVTGVLQGCLLVMGITFELRARKKGKAGLGGVAGDSNEHANQMNGNGHGYADGDEDHERTGLLGNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIY3</accession>
    <name>A0A8H6FIY3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007448</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156974.1">
        <property type="nucleotide sequence ID" value="XM_037298339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIY3"/>
    <dbReference type="GeneID" id="59335847"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="115" mass="13330" checksum="A8AEFFD98BCC428B" modified="2022-01-19" version="1">MVARFTHYNLRADFKKPLNESNFQCFGMEEPSVPEIVLHGLVSPYAHGEVLSHPVLHQNETLFATLNQAFRKTLVLAADQELTTAMRESRHEDCTPEETDYICREREKYKLLAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FIY5</accession>
    <name>A0A8H6FIY5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007516</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157042.1">
        <property type="nucleotide sequence ID" value="XM_037298407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FIY5"/>
    <dbReference type="GeneID" id="59335915"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053354">
        <property type="entry name" value="MGDG_epimerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43558">
        <property type="entry name" value="REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10540)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43558:SF6">
        <property type="entry name" value="REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10540)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="902" mass="102882" checksum="44C042F7BD5207D2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKG0</accession>
    <name>A0A8H6FKG0_9LECA</name>
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        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">HO133_004538</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
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                <person name="Allen J.L."/>
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            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157456.1">
        <property type="nucleotide sequence ID" value="XM_037295455.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="59332946"/>
    <dbReference type="Proteomes" id="UP000593566">
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    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="ACAD10_11_N-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACAD10-like"/>
    </dbReference>
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        <property type="entry name" value="ACAD10_11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829:SF1">
        <property type="entry name" value="HAD FAMILY PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="797"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="399"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="462"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="518"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="650"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="545"/>
            <end position="556"/>
        </location>
    </feature>
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        <location>
            <begin position="573"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="605"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="622"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="669"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="741"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="751"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="919" mass="101359" checksum="00A5AA721F9DFED0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKK9</accession>
    <name>A0A8H6FKK9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">HO133_004582</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157499.1">
        <property type="nucleotide sequence ID" value="XM_037295498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKK9"/>
    <dbReference type="GeneID" id="59332989"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11395">
        <property type="entry name" value="bHLHzip_SREBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052099">
        <property type="entry name" value="Regulatory_TF_Diverse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019006">
        <property type="entry name" value="Sre1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09427">
        <property type="entry name" value="DUF2014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="BHLH" evidence="3">
        <location>
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            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6230242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="814" mass="89928" checksum="1C2DE5DF03B406E7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYQ9</accession>
    <name>A0A8H6KYQ9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_007435</name>
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    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
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        <lineage>
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            <taxon>Ascomycota</taxon>
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            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
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            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156961.1">
        <property type="nucleotide sequence ID" value="XM_037298326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYQ9"/>
    <dbReference type="GeneID" id="59335834"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6229319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="206" mass="23746" checksum="645EC6C74C798C81" modified="2022-01-19" version="1">MYSLIFVKPTFIGAETKITKPFYIDAITWRNLAFAGTRRQIWNESLRVYPVKNGFEFFYIRPFLEFLEKIGICGQSPNFGAKTAFGEFETGSLASLSENLENVKWEVSGDYRRYDLESIFENGLCHWKYKMIDEKKQRQKKAELNRQEEEARSEHQGDGRVEASSNAQETNANEKHGDGYDEMIDDDDGYVSQNGNFMKDGVVVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KYX5</accession>
    <name>A0A8H6KYX5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannan polymerase II complex ANP1 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_007497</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157023.1">
        <property type="nucleotide sequence ID" value="XM_037298388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KYX5"/>
    <dbReference type="GeneID" id="59335896"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF2">
        <property type="entry name" value="MANNAN POLYMERASE II COMPLEX ANP1 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="446"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="446"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="470"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6229381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="60077" checksum="7350B39205FBD194" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZ04</accession>
    <name>A0A8H6KZ04_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007498</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157024.1">
        <property type="nucleotide sequence ID" value="XM_037298389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZ04"/>
    <dbReference type="GeneID" id="59335897"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="904" mass="101247" checksum="988C500777F5C0DB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZW1</accession>
    <name>A0A8H6KZW1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase II subunit RPB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_004712</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157627.1">
        <property type="nucleotide sequence ID" value="XM_037295626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZW1"/>
    <dbReference type="GeneID" id="59333118"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07031">
        <property type="entry name" value="RNAP_II_RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="9">
        <location>
            <begin position="24"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="274"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6230370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="338" mass="37311" checksum="8DD24349B135E1DA" modified="2022-01-19" version="1">MDYDNMDVDEVGPRVTVREAATDHVDFTLSNVDLAFANSLRRTILAEVPTMAIDLVEVEVNTSVLPDEFIAHRLGLIPLNSRQIEDVLYTRDCDCDQYCENCSVTIMMNAKCSKDNEDVVKVYARDLIVSEPRPNEWVGNPVITDEAGQGSVICKLRKGQEVIMKCIAKKGIAKEHAKWAPTSAVGFEYDPHNKLKHLDYWYEEDPIKEWPKSKNAEWEEAPQDGEPFDYNASPSQFFFELESIGNLEPDAVVQQGIKVMQQKLAAVLQELTGDDSRPGEADADGFGGQSPDGMPQGGEYGVDQGYTTPYVNGGATSAWGGGATPYGATPYGQNGWNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KZX4</accession>
    <name>A0A8H6KZX4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor CWC22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pre-mRNA-splicing factor cwc22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004652</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157569.1">
        <property type="nucleotide sequence ID" value="XM_037295568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KZX4"/>
    <dbReference type="GeneID" id="59333059"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000004">
        <property type="entry name" value="pre-mRNA-splicing factor CWC22 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="MI" evidence="10">
        <location>
            <begin position="424"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="380"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6230312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="741" mass="83573" checksum="E35FAF5B0B69FBEB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L003</accession>
    <name>A0A8H6L003_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">INO80 complex subunit B-like conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_004804</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157718.1">
        <property type="nucleotide sequence ID" value="XM_037295717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L003"/>
    <dbReference type="GeneID" id="59333210"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029523">
        <property type="entry name" value="INO80B/Ies2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561:SF12">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04795">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01406">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="2">
        <location>
            <begin position="325"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6230461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="434" mass="47324" checksum="04DEC498A62AE7F3" modified="2022-01-19" version="1">MAERPPNKRQRSMSYRQDGTIDGDGHSNTTPPAPPASTRPRRGASGSTVKVTRDESSSPDESRKALRLTVKMPSSKLREAMSSSTSSRSPNPGDQLTGAEILSGPRSSRAKKSYVVESESDDDEDDEDEEVEASDDEQIEQEDEEQDGEGEGEEDEEEDAEGEAEEESADEDADADGDIDMDEDADDGQPLPAPPSILKVNGPAAKTPAKPAAKPTLTVTPAQDAKVKSVEAKEMEMEDDDEELSELSENDDEEDAEGEEDAEGDDDVDMEQEDEGRSDAGSRGSTPDVSKMTKRQQGRLNQVVGSDFLQLPMEPQTKKHLTAEEHAMRRAEMARRRKNLSEKRNEEEKMDTINRLLKKQAPKRRGKISAAEITAQDRGDDSASPPEEEIAPANPVFVRWISNRDGCRVGVPGEWLSGPVGKVFGGGRAVEEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C675</accession>
    <name>A0A8H6C675_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_006922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000029">
        <property type="protein sequence ID" value="KAF6217406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146841.1">
        <property type="nucleotide sequence ID" value="XM_037297818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C675"/>
    <dbReference type="GeneID" id="59335322"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6217406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="354" mass="39803" checksum="9A406936448C4EDA" modified="2022-01-19" version="1">MANPAGKAILTVLGVFGSMVGFTGFLPNEFPNKEIHMVDRQQQAQDSMVRVVVGLNGVEGLTEADGPIPAVVAFDENKKYIGASDWSRGFGSIGSGGYADIWVSQKKGPGRQATYLQLLGHNDGICIAYISQVWGDGTPRGWLGDMGQACGKRYYYSNIVVGDHDHRPYCTWLNRDAGSDGSDKLEHIEAAAMQIHMEAFTNFTEDYNTDPGFYCREPAMLFRRKFSYELYHDDYEFDGLPDPGRIHFWYAKDADSMPRKKRSLPPRRSSRFDGRLITSVHGRHSARQLCENDHSYGPDFVSYAENVFCDMGTREHWPLCDGPVEDECYSMATRSLVTRSGYVKRSHVRVESWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6C1</accession>
    <name>A0A8H6C6C1_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147033.1">
        <property type="nucleotide sequence ID" value="XM_037297597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6C1"/>
    <dbReference type="GeneID" id="59335101"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="407" mass="43861" checksum="E459FC45425A6CEE" modified="2022-01-19" version="1">MAIKYRAAEMSISIDQVPADDPNLSVPGEIAAPGQSSVPTAFVTDAPAFATKSSTTLSTPSSTAATSSQSSTAAAPTQSAAAFKGGSGLSHGDIAAIVIPIALLAILIPILVLWYLDRKNRTAAEKRASHRSSNEAMLQKHSSIQKPPQPRGPKPARPERTPRRSVVDPPTPETRNSLGLFNFELSPPSTPGMGWMTPNPRFSIARALQLRRSQPSVVTSQPRTSRGESDRPRTGDSERSERRQTRTSIFDPPPPYVIDTASPKSASRFAPLERIGTLQHADRPHGPTMQPTMAPTLPAVAITNGALDVQQPRYTPRHTPRASSEILQLPDAYGRVHTRSPSPTLSMHNGSNLSRPFSYAAPERISDVSGLSDEPEPWRRMRESNGSSVISPIDSDESSTIHPHQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6I5</accession>
    <name>A0A8H6C6I5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fungal N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147138.1">
        <property type="nucleotide sequence ID" value="XM_037297428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6I5"/>
    <dbReference type="GeneID" id="59334931"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="48500" checksum="87F3C54DCE36A2D2" modified="2022-01-19" version="1">MDPISAAASIIGLLGAAAKVSDVLLKFIGSVKGAPKLAQNVLMEISDVSACLNQLQRYLQGALSTSNSQEQLLMVEQLVVTLSNCVLIFSELEEAVDSLKPAEPMQSWRLAQWLFKEQAICALMLTTLIQQVLESNLEMSQRMANLEMRTFGLSRSAAPTLAALNTSRDDDSIKTMRAANDVQEKVLGEHFETIEETNQKADKESTNDQGPTFSFTFDQDLNSSRPYTRAMKRNSVWSTAPSTVHTMSWSCLSGLSLADVSEISVIGLPISPQELWNGHHYILANSDMNDVPEKPLAPNRDDLAYRPDESFYEDESEPLETRKGLFPYDNAQSVGGSLDLRSVDGQGRKIPIAAGGRLLEPKKIILLGATMAGKTTTYHRLQMTHGNDFTDSDRRRARESVIHGLVDVFKKARGQYQDPISIEDIEVCSRMNIPIICKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6M0</accession>
    <name>A0A8H6C6M0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7923 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147004.1">
        <property type="nucleotide sequence ID" value="XM_037297568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6M0"/>
    <dbReference type="GeneID" id="59335072"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057683">
        <property type="entry name" value="DUF7923"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37543">
        <property type="entry name" value="CCCH ZINC FINGER DNA BINDING PROTEIN (AFU_ORTHOLOGUE AFUA_5G12760)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37543:SF1">
        <property type="entry name" value="CCCH ZINC FINGER DNA BINDING PROTEIN (AFU_ORTHOLOGUE AFUA_5G12760)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25540">
        <property type="entry name" value="DUF7923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7923" evidence="1">
        <location>
            <begin position="71"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="160" mass="18092" checksum="896CC85D1EC41171" modified="2022-01-19" version="1">MASKDKDLSLVDYRQRLIYFKDADAERNKSERGQRRILQSRVEDLEALTVSRSGRFKGGPANREPFLKSPGFILVLIDADADDYAFHEKFLSRNEAGGRDAADELQARTKDYVKNIKLDAENTDITVRAYADLKSLRSACVRNGRMKESSSMSLFAHGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6N0</accession>
    <name>A0A8H6C6N0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Potassium channel tetramerisation-type BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147322.1">
        <property type="nucleotide sequence ID" value="XM_037297197.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59334700"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18316">
        <property type="entry name" value="BTB_POZ_KCTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758:SF2">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="2">
        <location>
            <begin position="1107"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="707"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1476"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="746"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1579" mass="173793" checksum="EF93C652B12E2BC9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6V4</accession>
    <name>A0A8H6C6V4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_006387</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147410.1">
        <property type="nucleotide sequence ID" value="XM_037297285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6V4"/>
    <dbReference type="GeneID" id="59334788"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051694">
        <property type="entry name" value="Immunoregulatory_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549">
        <property type="entry name" value="PAIRED IMMUNOGLOBULIN-LIKE TYPE 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034457888" evidence="7">
        <location>
            <begin position="25"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="140"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="218"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="222"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6217975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="283" mass="29504" checksum="F55C24D86F965779" modified="2022-01-19" version="1">MMFSLCPHCALVSSLAIIARIASADSGANGTWLYPDPSTTPTFNYLDTLNASWTSDFSTPWLHLYCGVINGNSSSPVLGELYNQTAPATGSLDFPLNYALDPFSCHFELSRQPAVGLGPPYALSGTFNVAVESNAKPVTWSQNTGISSTADTGPSASTNPPSTASSTTSPKSTAGAEASGSNGLSQGAKIGLGVGVALGVLALIAAAAGFFMLRRRRNSSTRRTENGISHRGDQNLATPHPNAPGYATSKPGWSEMPGDAKVHEKDSVPVPRQTIGELEAPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7D5</accession>
    <name>A0A8H6C7D5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147426.1">
        <property type="nucleotide sequence ID" value="XM_037297301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7D5"/>
    <dbReference type="GeneID" id="59334804"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="96" mass="10847" checksum="2B2D5BBA5B21155B" modified="2022-01-19" version="1">MSPVFTAIHELTPPLRSLIQSRGRSLHTPGKVERRMTDEDCAASESRQHSDERIWLAWVAERREARAAGERHKAPPYADADKSVSVNVVRDLDEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C885</accession>
    <name>A0A8H6C885_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Chromatin remodeling factor mit1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_005775</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147861.1">
        <property type="nucleotide sequence ID" value="XM_037296680.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59334180"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17919">
        <property type="entry name" value="DEXHc_Snf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056616">
        <property type="entry name" value="Chromo_MIT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055565">
        <property type="entry name" value="DUF7141"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040934">
        <property type="entry name" value="Znf-CCCH_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041684">
        <property type="entry name" value="Znf-PHD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF17">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23615">
        <property type="entry name" value="Chromo_MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23614">
        <property type="entry name" value="DUF7141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18585">
        <property type="entry name" value="zf-CCCH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15446">
        <property type="entry name" value="zf-PHD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="369"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="757"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="1063"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="188"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="275"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="450"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1326"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1541"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="457"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1361"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1541"/>
            <end position="1556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6218426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1590" mass="179266" checksum="566AF18C6F5F2F09" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8A0</accession>
    <name>A0A8H6C8A0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148119.1">
        <property type="nucleotide sequence ID" value="XM_037296938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8A0"/>
    <dbReference type="GeneID" id="59334440"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="576" mass="61432" checksum="34F01652AA7787FF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8B5</accession>
    <name>A0A8H6C8B5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8212 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147906.1">
        <property type="nucleotide sequence ID" value="XM_037296725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8B5"/>
    <dbReference type="GeneID" id="59334225"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058525">
        <property type="entry name" value="DUF8212"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622:SF10">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26640">
        <property type="entry name" value="DUF8212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8212" evidence="1">
        <location>
            <begin position="94"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="247" mass="27649" checksum="146DCFDBFB9A86B9" modified="2022-01-19" version="1">MYQWYERADVCYIYLVDLFRHYLDDTSRLEDFDRSSPQATSIPLNPLAQPKSAGVAAKTSWAFQRDTARVEDIAYCLMGIFDVNMPLLYGEGDRAFLRLQHEILRASEDKSLFAWGSTRVTAARNPTWTYTTQGAAIDEFLGMSEDESLFAYRNATLASRHTGILAPSPGAFREGGDLITVKHPTLANSQSLYHDLVHPDASTEPPTLSQMAALAAKNAHPSITEEYGAYDQRKLDGVVSHRLAPWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8J2</accession>
    <name>A0A8H6C8J2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Queuine tRNA-ribosyltransferase accessory subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Queuine tRNA-ribosyltransferase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_005455</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of a catalytic subunit and an accessory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the queuine tRNA-ribosyltransferase family. QTRT2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148347.1">
        <property type="nucleotide sequence ID" value="XM_037296365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8J2"/>
    <dbReference type="GeneID" id="59333861"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:tRNA-guanosine(34) queuine transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03043">
        <property type="entry name" value="QTRT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028592">
        <property type="entry name" value="QTRTD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050852">
        <property type="entry name" value="Queuine_tRNA-ribosyltrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="7">
        <location>
            <begin position="30"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="413"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="459"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6218912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="473" mass="51920" checksum="C9EFDE3144E33D89" modified="2022-01-19" version="1">MPAANPHFMAGHSSKLVSFQLSRPQSDKDRPRVGRLTFQGRNPIDTPHYVAVSSRGAVPHLSQDMTRDNTAIRALYTAVEEFVEKASHQSQVPPVFNFPASSDESPLRKFIALQKDALLILGTRRVPPLPCPASSTSSAISIITSLGVGMLEYTDYAEAVQKLRPDIVLAMGDVLFGHKPGMKRKVVMGDRTLAWLKELISAMENEEDGTPRTALFAPILPIEAEQQNYYLGALQDELRDSVSGLVLYDAASVDAIPDNLRGLPRLSLGDATSPHKLLDAMALGMDAFTIPFITDATDAGVALDFSFPAQSNANFEGRLPLGIDMWSSTFANDISPLLTDCPCYTCTNHHKAFVQHLLNAKEMLGWVLLQLHNHHIIDEFFAGARDSIQSRSFEGDRVLFEKIYAAELPAKTGQGPRVRGYQFRSEGKGGPRKNQIAYRPLDDGKEKLAEAALPSPDADAEHLEEQGFAEKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8K1</accession>
    <name>A0A8H6C8K1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">GPI inositol-deacylase</fullName>
            <ecNumber evidence="4">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005985</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 4">Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GPI inositol-deacylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148069.1">
        <property type="nucleotide sequence ID" value="XM_037296888.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59334390"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054471">
        <property type="entry name" value="GPIID_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF16">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22939">
        <property type="entry name" value="WHD_GPIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="6">
        <location>
            <begin position="117"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="8">
        <location>
            <begin position="479"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase winged helix" evidence="7">
        <location>
            <begin position="757"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1699" mass="190013" checksum="C97F0D94B49D8BC5" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C988</accession>
    <name>A0A8H6C988_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148444.1">
        <property type="nucleotide sequence ID" value="XM_037296462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C988"/>
    <dbReference type="GeneID" id="59333959"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF73">
        <property type="entry name" value="HET DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G01020)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="66"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="612" mass="70278" checksum="4345BAF7EBAAD07D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9I1</accession>
    <name>A0A8H6C9I1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_005615</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6219071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148506.1">
        <property type="nucleotide sequence ID" value="XM_037296524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9I1"/>
    <dbReference type="GeneID" id="59334021"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06179">
        <property type="entry name" value="MFS_TRI12_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010573">
        <property type="entry name" value="MFS_Str1/Tri12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053791">
        <property type="entry name" value="MFS_Tri12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF195">
        <property type="entry name" value="PEP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06609">
        <property type="entry name" value="TRI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="449"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="540"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="58"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="598" mass="64912" checksum="2D56D97D06A95840" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9Y8</accession>
    <name>A0A8H6C9Y8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148819.1">
        <property type="nucleotide sequence ID" value="XM_037296121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9Y8"/>
    <dbReference type="GeneID" id="59333616"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6219384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="273" mass="30988" checksum="1C801F9BE3DCDF63" modified="2022-01-19" version="1">MISPYDPSGKIYTILSEEWTTCLWFFEPVSIPQVFREHLVLVLGWTTGRRGYVSIAMIAPNIPNGANPDHYFRIRSPRRRAVANEITVTLRKNKRFSALVELSYVRLDWVWEVPFATLLVVPRTGLQLMLRAVSMEKIMRFYSKWGWNQAWGQTYRPLPDCLQWAGYCHDQNTLGPPESVKQGGEAEYPEMTRQPCHVLNGLASSGTVEHDRGVKDEALAGSDSTRSWGSAHRPDDEEDGYLEEEASPCARQEMIYAGNAHCAALAKLLREAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAA1</accession>
    <name>A0A8H6CAA1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_003958</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148926.1">
        <property type="nucleotide sequence ID" value="XM_037294879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAA1"/>
    <dbReference type="GeneID" id="59332368"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF47">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="102"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="724"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="739"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="755"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6219491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="805" mass="89596" checksum="238B29C8BCDA8C65" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAL1</accession>
    <name>A0A8H6CAL1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin fusion degradation protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_003754</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149364.1">
        <property type="nucleotide sequence ID" value="XM_037294676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAL1"/>
    <dbReference type="GeneID" id="59332165"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.50:FF:000001">
        <property type="entry name" value="Ubiquitin fusion degradation protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055417">
        <property type="entry name" value="UFD1_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF13">
        <property type="entry name" value="UBIQUITIN RECOGNITION FACTOR IN ER-ASSOCIATED DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03152">
        <property type="entry name" value="UFD1_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 1" evidence="5">
        <location>
            <begin position="33"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="6">
        <location>
            <begin position="133"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="215"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="281"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00074895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6219929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="404" mass="43558" checksum="182B940102A20217" modified="2022-01-19" version="1">MFNPGFYQDDDPMSGMFPPGMMGRAGGMPPRRFDEYYRCYPVAMLPGPERENVNHGGKVIMPPSALDKLTRLHITYPMLFELINGNKDTMTHAGVLEFIAEEGKIYLPYWMLETLSLEPGDLLQIKSTDLPSGQFVKLQAQSTSFLDISDPKAVLENAFRNFSCLTVGDVFTFAYNDSVYSMKVLETKPGIAAKGAISVLETDLEVDFAPPVGYEEPKRVSGTSTPRSMTGALPPGGTLHNTGTMSKAINYASIAPTSSTAADGAKAVSSNFLLGGQKLNVRKGSKAPTPKASTPVAGASTNPPPTTIRRTNGPQPLRLPPGKLFFGYEVKPVRKTGEQKEKVEDESKPKFQGQGQTLRGGLKRKGGEPDAVEKIEKGKEAEKKDPGAGGGQKLGGRTLRDAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CAS0</accession>
    <name>A0A8H6CAS0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone chaperone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003810</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms a chaperone-bound H2A.Z-H2B complex that acts as a source for SWR1 complex-dependent H2A to H2A.Z histone replacement in chromatin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms a heterotrimer with H2A.Z-H2B, stabilizing the association of the histone dimer. Also, with a lower affinity, forms a heterotrimer with H2A-H2B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CHZ1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000018">
        <property type="protein sequence ID" value="KAF6219985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149420.1">
        <property type="nucleotide sequence ID" value="XM_037294732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CAS0"/>
    <dbReference type="GeneID" id="59332221"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019098">
        <property type="entry name" value="Histone_chaperone_domain_CHZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09649">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01082">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone chaperone" evidence="8">
        <location>
            <begin position="60"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6219985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="122" mass="13359" checksum="BCC066A300FBA3F5" modified="2022-01-19" version="1">MSSTNPTHGGTMSDPAQTVAEGKGKGKATDAPHDVSMGEDDSSSDEETGAEDEPKEEPEEEDEDNMEEIDTDNIISDGRRTRGKSIDFAKAAEEAGDELDDDDDDEEDDDFEEKEDDHEMKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CB52</accession>
    <name>A0A8H6CB52_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Symplekin/Pta1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_003328</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149632.1">
        <property type="nucleotide sequence ID" value="XM_037294251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CB52"/>
    <dbReference type="GeneID" id="59331739"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021850">
        <property type="entry name" value="Symplekin/Pta1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032460">
        <property type="entry name" value="Symplekin/Pta1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245:SF20">
        <property type="entry name" value="SYMPLEKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245">
        <property type="entry name" value="SYMPLEKIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11935">
        <property type="entry name" value="SYMPK_PTA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Symplekin/Pta1 N-terminal" evidence="5">
        <location>
            <begin position="88"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="699"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="526"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6220197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="760" mass="84299" checksum="11666992428D5F2E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBB5</accession>
    <name>A0A8H6CBB5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thiamine pyrophosphate enzyme N-terminal TPP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_003419</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149722.1">
        <property type="nucleotide sequence ID" value="XM_037294341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBB5"/>
    <dbReference type="GeneID" id="59331830"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF30">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE ISOZYME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="8">
        <location>
            <begin position="13"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6220287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="196" mass="21770" checksum="A71DC5CA4CF4C017" modified="2022-01-19" version="1">MILRVKGCDHASDYELALLDLIPNAGLEWKGSPNDLNAAYAADGYSRISNGIGALVTTYGPGERSALCSIAGAYCEYVPVLHIVGYPSVETMKAHKIMHHSLGYKNKAYQVRTCKEWDDLLREKTLDEASCAQLVYICNMHRVMRLEELVLDSILVDEQQRYPRDTHVDPSTSIVAASARLSAMDMVPPVRIFIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CC41</accession>
    <name>A0A8H6CC41_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_003140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150142.1">
        <property type="nucleotide sequence ID" value="XM_037294064.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59331552"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF63">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="246"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="670"/>
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        </location>
    </feature>
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            <begin position="1070"/>
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        <location>
            <begin position="1276"/>
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        <location>
            <begin position="1418"/>
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        <location>
            <begin position="1466"/>
            <end position="1509"/>
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    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="359"/>
            <end position="454"/>
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    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="495"/>
            <end position="589"/>
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        <location>
            <begin position="82"/>
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        <location>
            <begin position="231"/>
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        <location>
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        <source>
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        <source>
            <dbReference type="EMBL" id="KAF6220707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1698" mass="188106" checksum="9BBEDA486FA096C1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCA8</accession>
    <name>A0A8H6CCA8_9LECA</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">HO133_003062</name>
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        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
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                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037293986.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICASE_CTER"/>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
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        <location>
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            <end position="422"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1155" mass="129067" checksum="27050AB7EDD8BBAE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCE4</accession>
    <name>A0A8H6CCE4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_003129</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150131.1">
        <property type="nucleotide sequence ID" value="XM_037294053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCE4"/>
    <dbReference type="GeneID" id="59331541"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF20">
        <property type="entry name" value="[3-METHYL-2-OXOBUTANOATE DEHYDROGENASE [LIPOAMIDE]] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="281"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="314"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6220696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="444" mass="48672" checksum="A4BCFF7B1D0847E5" modified="2022-01-19" version="1">MPAMPARPAILDPCLIRIVCPLKQRSRRLLATATHQLPRNQPWSHPPASTSIDNDQVARLASRPLHPLTLADLVQHGRPPLSASTLFTSANFTLSLLPIRLAHRIQALRNLPFIVVSNPHISRIYNNYVHSLSTLLPYQERQINTLEDEIQFTEVMADLVQTHADTIPILARGFLECRKYISPGDVTRFLDEHLRARIGTRLIAEQHIALHLSSQPHCTNNDQYGQDGSSTSIGVIDTALRPAEIIRQCESFVSEICELKYGVRPTVIINGQPDTRLAHVPTHLEYILTELLKNAFRATVESGKERDPIEVTIAAAPDPSPSPPESIHLDPSRADPTTSNTPPGVTIRIRDHGGGISPENLDNIWAYSFTTFGDEELLDLGNGNADALNAISGAGGDSSSLAGLGYGLPLGRAYAEHFGGSIMVQSLWGWGTDVYLKLRGLGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCK5</accession>
    <name>A0A8H6CCK5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HO133_002708</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150462.1">
        <property type="nucleotide sequence ID" value="XM_037293633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCK5"/>
    <dbReference type="GeneID" id="59331120"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044912">
        <property type="entry name" value="Egfr_JX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051694">
        <property type="entry name" value="Immunoregulatory_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049328">
        <property type="entry name" value="TM_ErbB1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549">
        <property type="entry name" value="PAIRED IMMUNOGLOBULIN-LIKE TYPE 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21314">
        <property type="entry name" value="TM_ErbB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Epidermal growth factor receptor-like transmembrane-juxtamembrane segment" evidence="14">
        <location>
            <begin position="76"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="105"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="310"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="338"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF6221027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="507" mass="54407" checksum="A5D87A7C65DB77FF" modified="2022-01-19" version="1">MAAATTTSNPLNDFIGAAGAAASVVKSVNSVETPPTSSLSSTSSSPSATAAPAPAATSAVAGAHHGLSEGAKIGIIVGCVVGGLLLLALLGLCCCCLLRRRRRSKRRPTVTPVEDEEVKSWKSPINPGRHFSNYSPAQRGEVPTEQSPTVPLMAAAAAEHHVHNEKAPSLSQHPAMRHHENPFVPEPPSSRRTAPNSRAGLTDGMIPGAAAYVLPEKPGSRKSSSRSRSNSDTRPQSSGLPTHNDADRPPTPFGLTGFGEPAGHTIHRDHGVSPPYSGIGQPYEHQHVHNLQVDAPSRELRQSLRNHEVFPGAAFADDSHPKYRDSRGYTTPPEVPSRSPNRNNRASTLADSSHDSELSTTTASNSSGERYQSQIDPYQPAQRETVAPWEQHQKRFANSPQSSPHVAAPPPIPWEDSEFNIHSRKHQSHSPRQSGQYPSGDGRRRSSRSPATSINGQPRRLRFEDLQADNRASGGYSSVGQHDSYDGYNNNNSNNSNRWSQGVGEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCM8</accession>
    <name>A0A8H6CCM8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gfd2/YDR514C-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_002474</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150229.1">
        <property type="nucleotide sequence ID" value="XM_037293400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCM8"/>
    <dbReference type="GeneID" id="59330887"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040151">
        <property type="entry name" value="Gfd2/YDR514C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048519">
        <property type="entry name" value="Gfd2/YDR514C-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083:SF1">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21762">
        <property type="entry name" value="DEDDh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfd2/YDR514C-like C-terminal" evidence="2">
        <location>
            <begin position="201"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6220794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="384" mass="42911" checksum="3FBFF16AA0575FBB" modified="2022-01-19" version="1">MTVRGATQRESPDGHVLFIEEVRKHLGVRANAGESSSLADTTEARPKVLLIGHDMHDDFRKMRQDGIDLQKHFQYSGCVDTQVVVEDTGASMGKGLSDLMSHYGLAKLEFKKVGCPKMPGKWVFVGAHCSGNDAVAALECLIDQTFDLSLRTPGDKDSVGLEDLPEDWLKKPLQGMNMDLILLSYDTEGVETPNYKPKVLNRTSEHGFAWMRVADFAHTPPGDHGQNWRSFIRARHWINHDFRNFSNWFYCIGNPKGFWPKYGESQYYHVSEGPAPFHKLFEELANATAGALKGLDTVEEVTAMLEQTTLEEERTPAGGRNMAITRGNANNVRGNMTNSRSMQPPFRSNAKNIGEDTAEIRGNYSSWAEIAEGNEGKKFQQQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCM9</accession>
    <name>A0A8H6CCM9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome regulatory particle subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_002764</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150518.1">
        <property type="nucleotide sequence ID" value="XM_037293689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCM9"/>
    <dbReference type="GeneID" id="59331176"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189:SF50">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="108"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="141"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6221083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26174" checksum="5ECC4449B4F419F0" modified="2022-01-19" version="1">MDPEQHSKFAIHEAAREGRTAAVESLINANPRLATRRDDDERLPIHWAVSYNHMPVVELLVSRQDFDPDVQDASGWTPLMIAASLREGESLVDMLLSKEADVNAKNYSGQTALHFTASKNNLEIARRLISHGATARVKDKRGQLPLHRAAAIGSVPMVNLMLENKSPLNATDISGLTALHHAISEGHGDTALVLLKSGAETDKRDVDGNLAIDLAPDKKIRQFVLQSAEREGVDIEESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCZ1</accession>
    <name>A0A8H6CCZ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6221095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150530.1">
        <property type="nucleotide sequence ID" value="XM_037293701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCZ1"/>
    <dbReference type="GeneID" id="59331188"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF105">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1-ALPHA INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="1">
        <location>
            <begin position="130"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="301" mass="33722" checksum="A71919DE88988883" modified="2022-01-19" version="1">MIHNLSIVRTALPRINLRPIYTLTARWRSLRTLEDGSLHTFRDNAFIPSKPALLPKAHFIALPSIQKWFRTTQDGTGGVALNYSHLSKFGDAIVPLEFTRLSTASAANQYEDSFQRADVPLYIFLEWTKSASRETSERLYLAQASFSNLPEALKVDLPTPEIVANSGIGDIYDTNIWMGIPPTYTPLHRDPNPNLFVQLAGHKIVRILPPEIGDKVFEQVQNALGRSGSAAFRGEDMMKGEEMRLLEDQVWNDTSVLDEAGEGGYEAHVGRGDGLFIPKGWWHSVKGVDDGITGSVNWWFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CD73</accession>
    <name>A0A8H6CD73_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Synaptobrevin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_002119</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the USE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150699.1">
        <property type="nucleotide sequence ID" value="XM_037293046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CD73"/>
    <dbReference type="GeneID" id="59330533"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019150">
        <property type="entry name" value="Vesicle_transport_protein_Use1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050">
        <property type="entry name" value="USE1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050:SF7">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN USE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="127"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6221264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="30221" checksum="C02D896DFD1AC89F" modified="2022-01-19" version="1">MPLSSLNPRDVAAIDLGRVLSRLEHKILSADTADQRLQHSSYERIKVSANLEYARTLLLRLEHSSSSLKLPSQKASAQSSLSAQRALIKRLTDRLHDLEAHGDDNDLLYGPEDEEDILGEDAPAAPLAAQTREAEKPAAPAIEEPTSTLRNRKRPKNPPDGAIPTDEETTRAELLVHNDTESTHLTSSLLALAQSLKASSTAFSTSLEADTEILNRATEGLDKNSTGMEAAGKRIGLLKRMSEGKGWWGRMMLYAWIGGLWVLAILLVFAGPKLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE36</accession>
    <name>A0A8H6CE36_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150951.1">
        <property type="nucleotide sequence ID" value="XM_037293298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE36"/>
    <dbReference type="GeneID" id="59330785"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034324326" evidence="1">
        <location>
            <begin position="19"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="103" mass="11302" checksum="E922C9F0EE7DFC67" modified="2022-01-19" version="1">MYFAKSCLLLLASSAVLATALPAPLPNANLRREDDRVDVADGFKLRRDDDGVDVADGFKLRRDDEGVDVADGFKLRRDDEGVDVADGFKLRREDDGVDIADGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CE99</accession>
    <name>A0A8H6CE99_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein sip5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001968</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6222000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151435.1">
        <property type="nucleotide sequence ID" value="XM_037292896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CE99"/>
    <dbReference type="GeneID" id="59330382"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24139">
        <property type="entry name" value="SIP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039301">
        <property type="entry name" value="Sip5/DA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315:SF1">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="531"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="572"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="783"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="405"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="576"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="691"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="707"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="731"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="783"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="886" mass="95210" checksum="A0FC62DEDA8886E2" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEE4</accession>
    <name>A0A8H6CEE4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151116.1">
        <property type="nucleotide sequence ID" value="XM_037292577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEE4"/>
    <dbReference type="GeneID" id="59330063"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="296" mass="31655" checksum="53417438C28D6364" modified="2022-01-19" version="1">MNVGTDNETGTVSSAGVIFDTGPNPHSLSEQPPRPSCRVLAEAGQKPSQPTFEGIPVDIRAMIYNHILNTEADSSLRSASETRAQLSPRRGGPGLPTHTWHEMEPSADGATSQRPARPESRCARTRPSRVRVARDQPGKPQTSSAPTTTTSTTPACEAFPEPRSNLLTNLPRPVPFSDTQLHVMRSLGPDYRNSHHHHRTDAAANDMDNCIARNNIHIREACPSLTTFSLSIFSRPPLTPQSQARARTALALGELGKRPDRLSVVSTLPDSAVAVCARAIAPGADGEGCGVCTVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEL2</accession>
    <name>A0A8H6CEL2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Uricase</fullName>
            <ecNumber evidence="7 10">1.7.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Urate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_001754</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 10">Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>urate + O2 + H2O = 5-hydroxyisourate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:21368"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="EC" id="1.7.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7 10">Belongs to the uricase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.3.3" evidence="7 10"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151221.1">
        <property type="nucleotide sequence ID" value="XM_037292682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEL2"/>
    <dbReference type="GeneID" id="59330168"/>
    <dbReference type="UniPathway" id="UPA00394">
        <property type="reaction ID" value="UER00650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004846">
        <property type="term" value="F:urate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019628">
        <property type="term" value="P:urate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.270.10:FF:000001">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.270.10">
        <property type="entry name" value="Urate Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002042">
        <property type="entry name" value="Uricase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019842">
        <property type="entry name" value="Uricase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03383">
        <property type="entry name" value="urate_oxi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874:SF1">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01014">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000241">
        <property type="entry name" value="Urate_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00093">
        <property type="entry name" value="URICASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00366">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4 7 10" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6221786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="295" mass="32844" checksum="A7261E3E63EF3338" modified="2022-01-19" version="1">MPQVSAAQYGKDNIRVYKVHKDAKTGTQTVVEMTVCVLLKGDIETSYTKADNSVVVATDTMKNTVYIMAKQHPVTPPELFGAIVASHFVEKYKHIHSAWVKVVTHRWTRLTVGGEPHPHSFFRDGSETRNVEATADEGKGISIRSAIAGLLVLKSTGSAFHSFYKDEYTTLPDVYNRILSTEVDCGWTWSTFGSVAEVQSAVPKFDKAWESARTITLETFAKESSPSVQNTLYKMCEQILEAVSEVKAVDYSLPNKHYFEIDLSWHKGLKNTGKDAEVYAPQSDPNGLIQCTVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CET8</accession>
    <name>A0A8H6CET8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RFTS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001220</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151569.1">
        <property type="nucleotide sequence ID" value="XM_037292150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CET8"/>
    <dbReference type="GeneID" id="59329636"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022702">
        <property type="entry name" value="Cytosine_MeTrfase1_RFD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12047">
        <property type="entry name" value="DNMT1-RFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RFTS" evidence="4">
        <location>
            <begin position="42"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="237"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="496" mass="56127" checksum="39C45F670039A894" modified="2022-01-19" version="1">MAILEEDVLKPKDPSLRNSDEWPTFNFKKINVTSSKTGEQASLLSAHKGHPVTVSGKLDKIDSDLLPLVRDRRYHEKAIELKNVTTYAFAEYEDGSYGFWAAGKAGWFEIQSPASSYKETYSLMNEAASMFYMLADKLRRAIRKRPKLNAKELDKYTRALFKEYLASGKSLRLSDADDVREAFHEHRGFLITSMLECQEGLDWAQTPMLNYYESKFSDEYSKIEARVFGTNNALKAASLENRDNSKKRKSQSPHTEEPKDKKTRKGRAAAQVESQLPGTPPSKQVLDSASSDEDGVRNVLTRKHKSILQPSGGKTARKAAGRRKSLFAVADLSEGPHGEEQNDEDSQFFEGSPITTIHTAEDLELLTNPPRESSPEDAHGLKYLPRKYLELRVVEYDLPSMEPQGPGDLWTCTLEGCFHRVHEASKPAGKARIMEHFKTHATQAQEKIDLALNESRPYLPVNNLVRRLQAFAPDPGTELAPDTLQRKWQPPIRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEW1</accession>
    <name>A0A8H6CEW1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_001199</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151548.1">
        <property type="nucleotide sequence ID" value="XM_037292129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEW1"/>
    <dbReference type="GeneID" id="59329615"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="5">
        <location>
            <begin position="143"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6222113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="495" mass="55559" checksum="D65E81F32E19ABE9" modified="2022-01-19" version="1">MSAMALTAVDPQADQGDGRQKDKQAFIASPDVITTPCDPWPRPYYLEDGLRKVHPYHYTYNTYCKERWRGRELLDVFATEFRDRPQAYYKDAIERGNVVVNGVRVPTVKATVKNGDIISHTLHRHEPPVTAQPIGIVHEDDGMIVINKPAGVPVHPAGRYNFNSIIEIMRAERSHAWNPLPCNRLDRLTSGVMFIGKNARAAERLSMQIQGRSVKKEYVARVIGDFPEGDIVCEQPVMQISPKLGLNRARASGKEARTVFKRLAYYPPAQKFEDGKGKQEDNRSEKEKIRDSKMEWKGKEGHSIVRCRPVTGRTHQLRVHLQFLGHPISNDPIYCNQRVFGPSLAAGDALSDNDEDIISRLSRMGKSEVAEAMAYHDEMMDEYNKTKAEKMTGHLCDICDTPLYSDPGAHELGIYLHARRYACEEGKWDYETPLPDWALPPPGMAGPTEVTKETDPLHIVKGLETLEVSKDAGGKGKVLEQSGAEAGKLLWTHPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF20</accession>
    <name>A0A8H6CF20_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001355</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151704.1">
        <property type="nucleotide sequence ID" value="XM_037292285.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59329771"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="4">
        <location>
            <begin position="196"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="757"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="799"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="971"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1095"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1137"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1269"/>
            <end position="1310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1417" mass="157121" checksum="6041082FFC72D721" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFJ6</accession>
    <name>A0A8H6CFJ6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1275 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001546</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151895.1">
        <property type="nucleotide sequence ID" value="XM_037292476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFJ6"/>
    <dbReference type="GeneID" id="59329962"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010699">
        <property type="entry name" value="DUF1275"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37488:SF7">
        <property type="entry name" value="DUF1275 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37488">
        <property type="entry name" value="DUF1275 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06912">
        <property type="entry name" value="DUF1275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="28880" checksum="EF8E7B4B264B5D24" modified="2022-01-19" version="1">MALGSSDESAKTPQSPKPETISINRLKSHLCAELATSHADILLLTCCIISGLVDSTVYNAYGTFVSMQTGNTIFLGLGGATPHSTSKPYGWVKSFLSITSFCLGCFVFSLASRLLTPLRRSTLTASFVLQSLIIVVAAAIIQGGVVNGSLHTITADINWRELLPIALLSFQSAGQIVGSRVLGFAEVPTVVLTSMLHDISTDPALLGPLRQNMKRNRRILAFAGILAGAVAGGFISESTKRMQVPLWIAGGIKFFIASAWIVWPEKETNAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFY5</accession>
    <name>A0A8H6CFY5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-heme halogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000906</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavin-dependent halogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152201.1">
        <property type="nucleotide sequence ID" value="XM_037291839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFY5"/>
    <dbReference type="GeneID" id="59329324"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050816">
        <property type="entry name" value="Flavin-dep_Halogenase_NPB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006905">
        <property type="entry name" value="Flavin_halogenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43747:SF5">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43747">
        <property type="entry name" value="FAD-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04820">
        <property type="entry name" value="Trp_halogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="137"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="453"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="459"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6222855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="56994" checksum="912A3E62AF48400A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CG42</accession>
    <name>A0A8H6CG42_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000793</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152091.1">
        <property type="nucleotide sequence ID" value="XM_037291729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CG42"/>
    <dbReference type="GeneID" id="59329211"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000289">
        <property type="entry name" value="Acetyl-coenzyme A transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="498"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="528"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="566"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="644"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="686" mass="75865" checksum="1A21AB1514D073FE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGV8</accession>
    <name>A0A8H6CGV8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152491.1">
        <property type="nucleotide sequence ID" value="XM_037291056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGV8"/>
    <dbReference type="GeneID" id="59328535"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="140" mass="15295" checksum="F2CCDA9E1E4CC263" modified="2022-01-19" version="1">MSSIRAYTGAVGAGAYSATKGALEIKPTRHGRLLLSDQHLRPSNIKFGHPSIPDYAEFNKLYQAGVSALYSTQQGDPRKAADRIVDMVRSEGRTAGKSIPTRFPVGADAVEKIRGSCSKKMEICDEWEAFSSDTKFDPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHF2</accession>
    <name>A0A8H6CHF2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major royal jelly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major royal jelly protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152744.1">
        <property type="nucleotide sequence ID" value="XM_037291309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHF2"/>
    <dbReference type="GeneID" id="59328789"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017996">
        <property type="entry name" value="MRJP/yellow-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009:SF18">
        <property type="entry name" value="PROTEIN YELLOW-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009">
        <property type="entry name" value="PROTEIN YELLOW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03022">
        <property type="entry name" value="MRJP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034083318" description="Major royal jelly protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="47319" checksum="DFBDF835A8A2E070" modified="2022-01-19" version="1">MKLNILLAVGGLSCFAAAQSTQSITYQNLTYANVRRDSGLTGPPVEEVHYYYDQWPIGLAVSKQGRIFVCYTRGTYAYTLGEVFNTTAEVPYPNLELNTPPGGLSTSLNGIPFGSNSTDSFISVQALFITPDDTLWVLDTGRPSTNETEMLTMPYASPGGPKLVALNLTSNSIIRTYNFPSSVHFPDSYMNDLRFDMRANATASGKGIAYINDSSNEGRTGFIMLDLGTGQSWRQLTQHPSTLRTFEDVPSYQGMPFYQRSPGLALTNIQEGLDGIELSLYGDVMYYSPLTSDYLYSIETQYLRSNPMDDVLSSQRASNNVKNLGQRGGNANGFSADSNGAVYMLMPEQNAIYIYNSTTLQAEPYVRDPRIIWPDSSNVGFDGYIYFNINQLPYQPDWNNGTDLRQYPGLILRSKLPNNGTKSTVLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHF3</accession>
    <name>A0A8H6CHF3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000446</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152820.1">
        <property type="nucleotide sequence ID" value="XM_037291385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHF3"/>
    <dbReference type="GeneID" id="59328865"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="101" mass="10864" checksum="B2A1F56D8EDE5FFE" modified="2022-01-19" version="1">MAGVGEECFTLPAVTTSIYTLKVAACDVFGFHPSISKGAELLADAVVSMPDFLQGGLPLDAHPANTEEKRSILMHFIKAGQTRHGTQRSFLISKGSRREMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHG7</accession>
    <name>A0A8H6CHG7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 SUMO-protein ligase pli1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_000293</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152667.1">
        <property type="nucleotide sequence ID" value="XM_037291232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHG7"/>
    <dbReference type="GeneID" id="59328712"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16792">
        <property type="entry name" value="SP-RING_Siz-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.780">
        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023321">
        <property type="entry name" value="PINIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038654">
        <property type="entry name" value="PINIT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031141">
        <property type="entry name" value="SIZ1/2_SP-RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF100">
        <property type="entry name" value="LIGASE SIZA, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G05240)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51466">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PINIT" evidence="11">
        <location>
            <begin position="107"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="10">
        <location>
            <begin position="296"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="73"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="382"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="81"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="382"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="520"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6223450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="59304" checksum="A55325090466E98D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHK4</accession>
    <name>A0A8H6CHK4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YOP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000338</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152712.1">
        <property type="nucleotide sequence ID" value="XM_037291277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHK4"/>
    <dbReference type="GeneID" id="59328757"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF161">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="171" mass="19689" checksum="867BFD5455E29837" modified="2022-01-19" version="1">MSFQDRTQHQIGQIDKELSKYPMLNNLEKQTSVPKVYAFLGLVGIYFFLIFFNIAGEFLVNFAGFLVPGYYSLEALFSASKVDDTQWLTYWVVYAFFSVFESVVNAVYWFPFYYTFKFVLVLWMALPQTGGAQIVFRSFIQPVFSRFFSQSGSTAANLRSQADQATKPHAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIF1</accession>
    <name>A0A8H6CIF1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010611</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153097.1">
        <property type="nucleotide sequence ID" value="XM_037301463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIF1"/>
    <dbReference type="GeneID" id="59339001"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019487">
        <property type="entry name" value="RAM_signalling_pathway_SOG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF46">
        <property type="entry name" value="LEUCINE RICH REPEAT-CONTAINING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10428">
        <property type="entry name" value="SOG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="93"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="233"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="379"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="565"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="634"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="838"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="565"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="577"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="653"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="930" mass="103132" checksum="711B94684039092D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIR3</accession>
    <name>A0A8H6CIR3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_010824</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153309.1">
        <property type="nucleotide sequence ID" value="XM_037301675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIR3"/>
    <dbReference type="GeneID" id="59339214"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF33">
        <property type="entry name" value="PROTEIN HOS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="150"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="213"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6224249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="28134" checksum="181E652DABD1EDC3" modified="2022-01-19" version="1">MLQSVELGSNQVVEAQVAAKSDKSNDPTIRCTIDTGKAAEQLGHYQFVGAASPWDVNDHGSSALYFAAVSGDVGLCRFLIEQGADPLFENQYHTSPPDKAADIAVSFGRINCTNTLQFKPLHKAVLGLVPLPLGPLLDCSTADINVQDREGRTGLSWAITRDDIESSRLLLGHGADVIKSDNRGFGPIHSAKSLPSLKLLLDHNLDIMSKTAVGATPLHFVSRYGRHDMIEPMVKAHADQNAEKERNIIHLAAIHGDRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIT3</accession>
    <name>A0A8H6CIT3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153066.1">
        <property type="nucleotide sequence ID" value="XM_037301432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIT3"/>
    <dbReference type="GeneID" id="59338970"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="333" mass="36286" checksum="1F8D54043EAE0F5C" modified="2022-01-19" version="1">MAMETAMETANGNGNGNGNGNGNDQGQRKWAWVTEMATEMGTEMGTEMDNGNGQRTTIKGNGNGYGQRKWQRKWQRPRATEMETAMETANGNGNGNGNGNGNGNGNGNGNGNGNDQGQRKWAWATEMATEMATEMATTKDNGNGHGQRKWQRKWAWATGMGNGNGNGNGQRKWATEMATEMATEKGMGNGNGNGNGNGNGNGNGNGNGNGNGNGNGNGNGNGNGKWERKWKWKRKWKWQRKWGWGLQATLTYPNDGDIFQMVLVGTLRLLGGIFSGRGGDGRMVAETWQCTVVVLWKCESAWLKRELDVDAKSVNERDGYRREEKDSKSWQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIU2</accession>
    <name>A0A8H6CIU2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_010595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153081.1">
        <property type="nucleotide sequence ID" value="XM_037301447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIU2"/>
    <dbReference type="GeneID" id="59338985"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="88" mass="9840" checksum="4735ABAF6CAE8AB5" modified="2022-01-19" version="1">MDMDFGVGSYEMKNILSALASLARYHDAEELSDLPMNQYTSSGVEALLDEGIAGGEMLNDVLVFHVVDLDDMVVEIHEEMIIERQPQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJR3</accession>
    <name>A0A8H6CJR3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_011078</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153561.1">
        <property type="nucleotide sequence ID" value="XM_037301927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJR3"/>
    <dbReference type="GeneID" id="59339468"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF84">
        <property type="entry name" value="VACUOLAR MEMBRANE AMINO ACID UPTAKE TRANSPORTER FNX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="461"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="527"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="66"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="557" mass="59134" checksum="5B813E58444E09FF" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJW3</accession>
    <name>A0A8H6CJW3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153745.1">
        <property type="nucleotide sequence ID" value="XM_037300928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJW3"/>
    <dbReference type="GeneID" id="59338464"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="404"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="430"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="581"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="806" mass="89084" checksum="4F4D81F2969C6B7B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKA4</accession>
    <name>A0A8H6CKA4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153944.1">
        <property type="nucleotide sequence ID" value="XM_037301127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKA4"/>
    <dbReference type="GeneID" id="59338664"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="155" mass="17891" checksum="070AFA234445B456" modified="2022-01-19" version="1">MRFKPAQIISPSYLFNVRILQVVLALIYLILLCYCGIHHGWWLNLHQPLGFGISASLLTILIAIPDILNRDFTLSNTLSTYLFHFLRLLFELLLIALWCAAFVTMLLPKGKDFRLLFHRPPYVEWICAVVLAAIELLVFVWSMVLVLQEKYKVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKE1</accession>
    <name>A0A8H6CKE1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Palmitoyltransferase</fullName>
            <ecNumber evidence="13">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_010207</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Palmitoyltransferase specific for casein kinase 1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="13"/>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153879.1">
        <property type="nucleotide sequence ID" value="XM_037301062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKE1"/>
    <dbReference type="GeneID" id="59338599"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="308"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="88"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="122"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="221"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="15">
        <location>
            <begin position="443"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="603"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="713"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6225012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="732" mass="81053" checksum="55541EBCA368F823" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKJ2</accession>
    <name>A0A8H6CKJ2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154010.1">
        <property type="nucleotide sequence ID" value="XM_037301193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKJ2"/>
    <dbReference type="GeneID" id="59338731"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF152">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="35767" checksum="A8AFDBC4DF1B5006" modified="2022-01-19" version="1">MSKSTGTVLVTGGTAGMGYECALKIAQQCPQYQVLIASRSNNDNAAVSINQRTGHRNVEYLTLDLSNLVRVRSFAADYAKKGYPQIKALVLNAGLQILDLNYTEDKIESTFAINHVGHALLFYLMRTYLAEDARILITSSGTHDPAQKSGLPDAVYNSAEELAQPTKETGKYSGRQRYATSKLCNIMWMYALHRRLERTSGKKWTVNAFDPGLMPGTGLTREAPAILQFIWHKIMPRTIWLLRLLVSPNIHSPRDSGASLARLAVGDDVKQVSGKYYEGQRPIPSSKDSQVVEKQEDLWEWTVERVAKDDQERRDFENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL16</accession>
    <name>A0A8H6CL16_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153998.1">
        <property type="nucleotide sequence ID" value="XM_037301181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL16"/>
    <dbReference type="GeneID" id="59338718"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000140">
        <property type="term" value="F:acylglycerone-phosphate reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF3">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE SRDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6225131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="114" mass="12495" checksum="EB3FCAC8DA8C8A3D" modified="2022-01-19" version="1">MSVLQPFPVPLPAGVRFPVKTFAKRQRAVVMAQRYVLITGCGEGGIGEALAREYQKKACTVITTVLPWESKDHLISAGIQCYELDVTDDASVQALERIVDKVTEGRLHVLVNNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CL76</accession>
    <name>A0A8H6CL76_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MaoC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154414.1">
        <property type="nucleotide sequence ID" value="XM_037300566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CL76"/>
    <dbReference type="GeneID" id="59338100"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044594">
        <property type="term" value="F:17-beta-hydroxysteroid dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13078:SF57">
        <property type="entry name" value="DEHYDRATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G00640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13078">
        <property type="entry name" value="PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="145"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="250" mass="27262" checksum="EC6FD7B39E95A720" modified="2022-01-19" version="1">MSGPGVGFEYPSQEVSWLKRDQLTNLPAFKHTDQEVIDFYARSAATPIANVPKFDSRRVVDGQRSMKFLKPLPPTSVGKKFELRSKVLGVYDKGKVGTVIETETVLAEKGGDVYTKAVGSAFFIGQGNWGGPKGPSTVNYPPPEGKKSDAVHILQTAKESAHLYRLNGDYNPLHATPEPGQEMGFGGAIMHGLFSWNSACHGLLQTLGGSDPANLREFEARFAAPVKPGDKLVTEAWRTGEVKDGWEERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM11</accession>
    <name>A0A8H6CM11_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154546.1">
        <property type="nucleotide sequence ID" value="XM_037300698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM11"/>
    <dbReference type="GeneID" id="59338234"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="371" mass="40307" checksum="F176F54843AEF5DE" modified="2022-01-19" version="1">MFSTFPAALIDPQQTVGPPSAVEDQPPKQIAGLEDEEELDQFRERNLNLHVGLQVAQECRRLLTEKHEKHHVLATEHERCKKLMQKAIENLSRGLHLSTRELANLGLTVEGVAPARQLGKLVVRPALSKLMGKFTVRVSLLAVQAEPQLSKPTSSTNLQFSSTPRSLSFFWPRAFQTEGLDAPKPPFPGSASGSIPCTSRPLATTPSPAVGTAAGLRNPPQVGELALSTFGDPEQTIVPGVPDTTCVLVSIPGFCNRAEQSFEETRLRRYAFYDGESEPLVTPKWPETTFGQDFSPQSNERNIGEERGGWEGQTPRSMDTAGASSERGREWGGAFKPAKPNGDNTANVTSASKAIGEHYTATPYQHSGSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMP9</accession>
    <name>A0A8H6CMP9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Meiotic recombination protein dmc1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154806.1">
        <property type="nucleotide sequence ID" value="XM_037299980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMP9"/>
    <dbReference type="GeneID" id="59337514"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031349">
        <property type="entry name" value="Tfb6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781:SF1">
        <property type="entry name" value="ADR380WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781">
        <property type="entry name" value="TFIIH COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17110">
        <property type="entry name" value="TFB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="29639" checksum="8BC85FFFE544F044" modified="2022-01-19" version="1">MSSAYGAPGPGGFLRPTLPSPPASSFNSPPAGYTILPQPRSTPLKPGSAKESSFIDYVDRKLLEISRRYEKRFNADFEDEAMSGIQGRGYESYGELARDLEGVIDVVWVSGTPSLQTPYLLTIALTSCTCLAPFPFSPRPTFHLLHKLDMAFYSLLQGANAETGEILSGFEGGRGKLSTTEKVRMRSLVERTRVAAVEVAGKGGSVTDTESVAQSSMETEDDFTTDNDEDDIMEEMQVNGNHGRWEMEIARVYEKTIVELGVSLDTSGMGGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNM2</accession>
    <name>A0A8H6CNM2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF300-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008273</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155141.1">
        <property type="nucleotide sequence ID" value="XM_037299140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNM2"/>
    <dbReference type="GeneID" id="59336670"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="349"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6226832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="44431" checksum="B8952F556AF69A86" modified="2022-01-19" version="1">MGLFSSSSSSDSTCPSIPTGPQYGADAVVGSLTFHTLTIILCGAFTAATFLFSGVQLFLHATHFSNPGQQTQILRIVALVPTFALVFFLAGFIPSAAIYLQPWADAYEAVALTSYFLLLVVYTVPDPRLREGFFDRLQQPKGGEGSLKWYRRTWCFVFQYLPISFIVAVATDITQATGTYCVNANSVHFAHIWLTIIHTISVLIALPRLLLFYKRLRTELKEHNVVPKLLAIKGIVALSTLQSVIFTILSSTSAVEPSSKLSYDDIYFGIPSILICGEMVFFSLFNFYAYSFRPYTLASSGSGMESQPLKSRAHYHGGFLGIKALLAAMNPTEIASGLVLAVRYLVSSPQPQSHDNVPLRQGVNASAPPPYMPQGRYQGLTNADRAHSPNGVEYGRVDRYSPLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNN4</accession>
    <name>A0A8H6CNN4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008451</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155318.1">
        <property type="nucleotide sequence ID" value="XM_037299317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CNN4"/>
    <dbReference type="GeneID" id="59336847"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="642"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="378"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="484"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="544"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="655"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="669" mass="74330" checksum="EAD3280C3E29FC62" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP29</accession>
    <name>A0A8H6CP29_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Succinate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="8">1.2.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_008386</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13217"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 8">
            <text>succinate semialdehyde + NADP(+) + H2O = succinate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.16" evidence="8"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155253.1">
        <property type="nucleotide sequence ID" value="XM_037299252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP29"/>
    <dbReference type="GeneID" id="59336782"/>
    <dbReference type="UniPathway" id="UPA00733"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010102">
        <property type="entry name" value="Succ_semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01780">
        <property type="entry name" value="SSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="57"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6226945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="531" mass="56962" checksum="2FF111C4838B0601" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP81</accession>
    <name>A0A8H6CP81_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Cutinase</fullName>
            <ecNumber evidence="3 12">3.1.1.74</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_008524</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the hydrolysis of complex carboxylic polyesters found in the cell wall of plants. Degrades cutin, a macromolecule that forms the structure of the plant cuticle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>cutin + H2O = cutin monomers.</text>
            <dbReference type="EC" id="3.1.1.74"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cutinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.74" evidence="3 12"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155391.1">
        <property type="nucleotide sequence ID" value="XM_037299390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CP81"/>
    <dbReference type="GeneID" id="59336920"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050525">
        <property type="term" value="F:cutinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000675">
        <property type="entry name" value="Cutinase/axe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043580">
        <property type="entry name" value="CUTINASE_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011150">
        <property type="entry name" value="Cutinase_monf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48250:SF2">
        <property type="entry name" value="CUTINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48250">
        <property type="entry name" value="CUTINASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01083">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00129">
        <property type="entry name" value="CUTINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01110">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00155">
        <property type="entry name" value="CUTINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034357107" description="Cutinase" evidence="12">
        <location>
            <begin position="18"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="60"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="194"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR611150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR611150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6227083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="231" mass="24626" checksum="515E6BE48CAA8315" modified="2022-01-19" version="1">MKRLFFCLAILASSVRAIAINMAHARRFEETGTASTTITKRQDDWDVERNELTSVAGVGCKPITVIFARGTIELGNVGELVGPPFFNDLDDLIGADNIAVQGVDYAATIAGYLIGGDPGGAQTTADLLNQAASNCPDTQIVLSGYSQGAMEVHLGEAMVSEEVAAQIAAVVVFGDPFKGRPFPNVDSSKVMTYCFNDDFICDDLPVVDTYHLEYVVDTPAAATFVQGLVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPB2</accession>
    <name>A0A8H6CPB2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_008814</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155678.1">
        <property type="nucleotide sequence ID" value="XM_037299677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPB2"/>
    <dbReference type="GeneID" id="59337209"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF104">
        <property type="entry name" value="EXONUCLEASE 3'-5' DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="4">
        <location>
            <begin position="207"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="67"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="474"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6227370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="621" mass="69255" checksum="E1B471E93D624D3B" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPF9</accession>
    <name>A0A8H6CPF9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_008659</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155525.1">
        <property type="nucleotide sequence ID" value="XM_037299524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPF9"/>
    <dbReference type="GeneID" id="59337054"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000001">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034748394" description="Peptidyl-prolyl cis-trans isomerase" evidence="4">
        <location>
            <begin position="29"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="39"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="277" mass="29858" checksum="124B54A31AA705AB" modified="2022-01-19" version="1">MANLQRVVISLLLILTASFLFLAQTSSAAKGPKITNKVYFDITHGDEELGRVVIGLYGKTVPKTAENFRALCTGEKGFGYEGSAFHRVIKQFMIQGGDFTNGDGTGGKSIYGNKFPDENFKLKHTKTGMLSMANSGKDTNGSQFFITTAITSWLDGKHVVFGEVLEGYDVVEKVENVPKGAGDKPAKTVKIAKAGELPVPEEELIGSALPAVPGDELDVTLGDEPKQQLETTESDVIPPTESHGLSILTKLIFFGVIVGVVLSFLRTRKPMVEKSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQB7</accession>
    <name>A0A8H6CQB7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Autophagy-related protein 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_007638</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ATG13 family. Fungi subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6227910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155844.1">
        <property type="nucleotide sequence ID" value="XM_037298508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQB7"/>
    <dbReference type="GeneID" id="59336035"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040182">
        <property type="entry name" value="ATG13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018731">
        <property type="entry name" value="Atg13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13430:SF4">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10033">
        <property type="entry name" value="ATG13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy-related protein 13 N-terminal" evidence="6">
        <location>
            <begin position="80"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="343"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="502"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="610"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="465"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="562"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="620"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="648"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="678"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="711"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="760"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="929"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6227910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="957" mass="103024" checksum="0F9E91E51E8D7D83" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CRR9</accession>
    <name>A0A8H6CRR9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156296.1">
        <property type="nucleotide sequence ID" value="XM_037298960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CRR9"/>
    <dbReference type="GeneID" id="59336489"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="344" mass="38330" checksum="7436117F4554B9E9" modified="2022-01-19" version="1">MPMPWIWSCHKCHTRYHLGATRRCLHDGHYFCGGTTVDKISGKVKKHRACVSEFDYSGWEDFARWKRATSRKGEKAGRKHCEDECDFPSSCHWKEQHPVQETGFGFLDPSCLDTEPDTSSVEGKLTVQESTGSYIGKIRRAAEKRTTKVAKALLSPIAEEDQKASSSLDPRPRMNGLGLHFPVMDFSSSKNSVKEIHGHVDKAKIKLSIPNSRQMSGRVVHVWEDDVDMTDWITPEATESPPISPCARPDAVEVPFDFGLEQDQGPPASVADDDSPVSPMRSAWNWTAGGIGVALSPPALPIEDEIWDEGMEDEMDEADMMWDRGETVKAESLILDALFDPVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F6B0</accession>
    <name>A0A8H6F6B0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione hydrolase</fullName>
            <ecNumber evidence="3">2.3.2.2</ecNumber>
            <ecNumber evidence="3">3.4.19.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Gamma-glutamyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Gamma-glutamyltranspeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_006902</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione conjugates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="3"/>
    <dbReference type="EC" id="3.4.19.13" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000028">
        <property type="protein sequence ID" value="KAF6217432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037146867.1">
        <property type="nucleotide sequence ID" value="XM_037297798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F6B0"/>
    <dbReference type="GeneID" id="59335302"/>
    <dbReference type="UniPathway" id="UPA00204"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000008">
        <property type="entry name" value="Gamma-glutamyltranspeptidase (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF62">
        <property type="entry name" value="GLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034900279" description="Glutathione hydrolase" evidence="4">
        <location>
            <begin position="28"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="401"/>
            <end position="403"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="453"/>
            <end position="454"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6217432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="574" mass="61645" checksum="B816F8148E46D645" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F728</accession>
    <name>A0A8H6F728_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147240.1">
        <property type="nucleotide sequence ID" value="XM_037297530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F728"/>
    <dbReference type="GeneID" id="59335033"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="178" mass="18872" checksum="E571C254BE1BA90C" modified="2022-01-19" version="1">MAVPPTDPNTTPPILTAAQTRALLHTLLSNAANAARPNMTSPGPKAVRQQPVQQQQQQQTITIDASTRIMGHCNNVALASPSPTEQAVKIEQLLRGVFQPAGPGEEKTKARANVSLNVNVQAGVSILGSKNVVVFNGKRDVGKDSTKGDEPLAGRKRRAESEPADIQMTLGKKVKVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7I3</accession>
    <name>A0A8H6F7I3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000023">
        <property type="protein sequence ID" value="KAF6218152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147587.1">
        <property type="nucleotide sequence ID" value="XM_037297013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7I3"/>
    <dbReference type="GeneID" id="59334515"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790:SF4">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="1">
        <location>
            <begin position="53"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="221" mass="25349" checksum="50EAA7BC692137A5" modified="2022-01-19" version="1">MVCTIKEWHPTTRWVDSEETEARRFQKLESDLFKPYKCDVSAPQKETMSRNYAASPLFRLPPKIRVRIYGLVLDGMQLWIAHSESKVELEKPEKNSQPQGPQSAQYGHYRHRCGRSYQSTADSFPRTKPSINVLHLGLSRVCGQVYTETALLPFTLNTFTFSDDLVRREFEQTARPGEKRVQKNAVGKYEIGAKENPAVVPFQSTHELQNNEIMNMVVLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7J7</accession>
    <name>A0A8H6F7J7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2293 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_006308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147331.1">
        <property type="nucleotide sequence ID" value="XM_037297206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7J7"/>
    <dbReference type="GeneID" id="59334709"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018744">
        <property type="entry name" value="DUF2293"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38113">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38113:SF2">
        <property type="entry name" value="DUF2293 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10056">
        <property type="entry name" value="DUF2293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2293" evidence="2">
        <location>
            <begin position="120"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6217896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="502" mass="56604" checksum="B93D79664BA5A0AD" modified="2022-01-19" version="1">MNSVGTSQPRALLKHAYERLSDSSDHPPSGYVFVPKGDVYITRHWNLTSVSGRWLWIVYDGKTNERLGIHCFQQIVEAVKEDAAGTEERVLNPSPQSEEKRQRAVAAKDSRDHEKAHKVLLEFFPNIPESACTEILGHGFQKGSGRVGRSQILEDQLKVHLAVNAHIRHRLTQYDSILAANKGQDAKLAAREMVYGEVQAIADSWRATSSQTWNSNSRTSVPRGSAATLEANWQRRTQQSIAQTTPANEAQVLEEALGGLHLNENQREVGARTEAAERRAQKVAQKLARKGRFRESTRDLLRQYELDPSIEMTKNKKKGVLRLQMEQKERRGKGKYAQKLHERQNPNPTNPKKREGYRRLKITANGVELEPRELDRYVPDDGPSDEQPHKPRLLRSNYRKPGDTPTSSGDLGDTPGDGVQLEARGHDRYVPNYGPNPSIDPPRKSRYPLRSSHQMSGDLDANNNKRLGLIEKTTDEGRLLVEDSEWTDIDNISLRTAGVHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F7R0</accession>
    <name>A0A8H6F7R0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147750.1">
        <property type="nucleotide sequence ID" value="XM_037296569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F7R0"/>
    <dbReference type="GeneID" id="59334066"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="121" mass="12338" checksum="8D21CBAC1EC75939" modified="2022-01-19" version="1">MAGSPSDASGGMSGALVATIVLTVLAAVAGGVYMSGAADDVVKFVMEKYFKAEAKAEEKMLEKSGETAAEGFLKDRLKKNPVVSNDELNQVSGGLGDEAAKEFGKDFGKGGLGQGIGKAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8J5</accession>
    <name>A0A8H6F8J5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_005950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148034.1">
        <property type="nucleotide sequence ID" value="XM_037296853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8J5"/>
    <dbReference type="GeneID" id="59334355"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052999">
        <property type="entry name" value="PTS1_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180">
        <property type="entry name" value="CONSERVED MITOCHONDRIAL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180:SF5">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6218599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="237" mass="26565" checksum="A9CCA38005EB6549" modified="2022-01-19" version="1">MAAKPESLPQLFDAVKAKFPESLGPDRWYLVAASSLITNSNPTNFAQLWTYLTAQGEYQALEKRQALNRRLREFLMKQWVTIGVPKSAVALFSLIKEEKPGDADLSFTKSHAALTPANRERGTQFLRYLYTEEQMEAMWKSWGKDSEWLTKEIVYGMFYGDDTILGFVDTELITYTAIMCQGLQVTIHNHLGGLLGMGLSVEEIEGVTECGELVAKWSGVDTSGWPDVREIAAGLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8N7</accession>
    <name>A0A8H6F8N7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_005261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000021">
        <property type="protein sequence ID" value="KAF6218719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148154.1">
        <property type="nucleotide sequence ID" value="XM_037296172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8N7"/>
    <dbReference type="GeneID" id="59333667"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6218719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="102" mass="11147" checksum="1B9E5C1763ABC4C0" modified="2022-01-19" version="1">MAGAHQQRVSVDEESGALWERADMEILLLDGYELAYYTLRRVHDGHLQSFLPVNTGATSVQAVPIDAQLSDIQALEGLQANQEGPESGSLEPICFSNHVPNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F8Z9</accession>
    <name>A0A8H6F8Z9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">tRNA (uracil-O(2)-)-methyltransferase</fullName>
            <ecNumber evidence="3 11">2.1.1.211</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_004074</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-methyltransferase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>uridine(44) in tRNA(Ser) + S-adenosyl-L-methionine = 2'-O-methyluridine(44) in tRNA(Ser) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10339"/>
            <dbReference type="Rhea" id="RHEA-COMP:10340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.211"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TRM44 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.211" evidence="3 11"/>
    <dbReference type="EMBL" id="JACCJB010000019">
        <property type="protein sequence ID" value="KAF6219605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149040.1">
        <property type="nucleotide sequence ID" value="XM_037294993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F8Z9"/>
    <dbReference type="GeneID" id="59332483"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141101">
        <property type="term" value="F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011671">
        <property type="entry name" value="tRNA_uracil_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210:SF0">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07757">
        <property type="entry name" value="AdoMet_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 11" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6219605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="605" mass="67914" checksum="426E0261919CADC6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6F9R6</accession>
    <name>A0A8H6F9R6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_003337</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149641.1">
        <property type="nucleotide sequence ID" value="XM_037294260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6F9R6"/>
    <dbReference type="GeneID" id="59331748"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6220206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="144" mass="15529" checksum="B8DBE956B9DC94B5" modified="2022-01-19" version="1">MVLDRVGLGDEKSFGKIISTSKDLSDKKHDLQQASTPGALASNSVKTVREVTRAAFGIYGDQQHLVDAIKKLTELDGISFPVATLILVEYDPVNIPYFTNACTDGCRKTTQGTGFGIGTSTIPSLNFSSKDKGDIQATKWSRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAB6</accession>
    <name>A0A8H6FAB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial inner membrane protease subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HO133_002876</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S26 family. IMP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000016">
        <property type="protein sequence ID" value="KAF6220444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149879.1">
        <property type="nucleotide sequence ID" value="XM_037293801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAB6"/>
    <dbReference type="GeneID" id="59331288"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037730">
        <property type="entry name" value="IMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041:SF2">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase S26" evidence="13">
        <location>
            <begin position="85"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="29"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF6220444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="248" mass="27807" checksum="2DFD71020095B809" modified="2022-01-19" version="1">MAPIALFTSMPTLLRPLRPISHLRNAARHLSVPSRPHRPPRSQPTGPPIPKIHPTATPTPTAPPRPRPARTTLSVLVFFASWFPAFSFLTNNVFCIMSVTGPSMSPFLNADYNSSMASDRVWVNMWRAGEGVERGMVVVYRSPANPERFVVKRIVGLEGDTVITKPPYPFTQEDVPFGHVWMEGDQLDGNKTMDSNRVGPVSKSLIVGRVEGVVWPWRKAGRIRWEDWKGSDRVMVGKGTVEEIQFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAI2</accession>
    <name>A0A8H6FAI2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_002637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150391.1">
        <property type="nucleotide sequence ID" value="XM_037293562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAI2"/>
    <dbReference type="GeneID" id="59331049"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="19360" checksum="E8132743C8DC987E" modified="2022-01-19" version="1">MTGCLRTWGYVEFETEAEAKNAIQGLKNGSFSTYGNHLRMKIVKAVGALPMDVDDWIGELAEGWAYKPKVADASDESKGVESQNPAKLYDPADVNTGDKGKAIASPSPAKLYDPADVDTNETGKAAESRSLGETHEDPDIDTGDKDQTLEPLTLFAIHRGTSVHTPDKDQLAVAPKERAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FAW6</accession>
    <name>A0A8H6FAW6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-mRNA-splicing factor cwc26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151187.1">
        <property type="nucleotide sequence ID" value="XM_037292648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FAW6"/>
    <dbReference type="GeneID" id="59330134"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070274">
        <property type="term" value="C:RES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018609">
        <property type="entry name" value="Bud13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051112">
        <property type="entry name" value="CWC26_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809:SF0">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09736">
        <property type="entry name" value="Bud13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="256"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="35669" checksum="8DF6B30808F2591C" modified="2022-01-19" version="1">MSLADYLAKNYLTADSKPQKKSKKRKRKDGAASGLIIADDDALGWDRDSTGNADDDAPLEVNANSAEFRKAKKTTWQTIGTPTPSSNEQAAADAILTFSAAESRARALADDDAPTMEDSAMKMESGAHAGLQTADQVTAQLKRKEAEERRRFLEDGDDHSGKGKETIYRDASGRIINVQMKRAEARKKAEEEAAKAAAELEGQKGDFQRAEKEKRREQLSEAKFMPVARYADDVELNEDLMERERWNDPAAGFLERKKEGKSVSGKPLYKGPATPNRYGIRPGHRWDGVDRGTGFEKEYFAARNRTANLKNLDYAWQMDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FB59</accession>
    <name>A0A8H6FB59_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Velvet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_002289</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000014">
        <property type="protein sequence ID" value="KAF6221433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150868.1">
        <property type="nucleotide sequence ID" value="XM_037293215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FB59"/>
    <dbReference type="GeneID" id="59330702"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3960">
        <property type="entry name" value="Velvet domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021740">
        <property type="entry name" value="Velvet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037525">
        <property type="entry name" value="Velvet_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038491">
        <property type="entry name" value="Velvet_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572">
        <property type="entry name" value="SPORE DEVELOPMENT REGULATOR VOSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572:SF18">
        <property type="entry name" value="SPORE DEVELOPMENT REGULATOR VOSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11754">
        <property type="entry name" value="Velvet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51821">
        <property type="entry name" value="VELVET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0749">Sporulation</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Velvet" evidence="7">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="283"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="447"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="404"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="462"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6221433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="506" mass="56465" checksum="FBA862DCDAF3F624" modified="2022-01-19" version="1">MRMRSNAYTLTVRQGPERAKAFAGPKEKDRKPVDPPPIIQLQIRDPQDPTQNYLQSPYFFMCCNLCDANLDRPGQLAAAHQGEDIDTATQSALAGTLVSSLHRLKDVDNTDGGFFVFPDLSVKIEGDFRLRFSLFEMGKTRVSYIKSVTSSPFTVFTPRAFPGMSESTFLSRSFGDQGVKLRIRKEPRVLGKRSAPSDFLPADAAHGHPQQTPVEYRNMGLQPQHEQSGGFGPSGYKEPTLGYDDLPLKRQRMSIDLARRTAYEHDQRLSQRSYIQQRDAYGQYAPSTPSRGSYYVQGPSTGPGPIEYPFSHGRTNSSSNSSPFLSPRTEYPAYQFPAPNSMYQTHSRNSPQQYQQSQYTPTQARPVPQLAQSLPPYRPLPSSVPSQPEQPRAYSRSMEADEQSLASRGYEQSYNNSQYPPINYDRQVQPLARTLPAPSQAVTSLLPPLQSLSSAQPRRDTLQSYSSGGMSNVETNTHLPSSLPLVPPSSEAQNFLPQPYRGRDHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FBH8</accession>
    <name>A0A8H6FBH8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151486.1">
        <property type="nucleotide sequence ID" value="XM_037292067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FBH8"/>
    <dbReference type="GeneID" id="59329553"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034354527" evidence="2">
        <location>
            <begin position="24"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="179" mass="19606" checksum="C4601824F3D2B835" modified="2022-01-19" version="1">MRPLWSPHLLLLTFLSLTSLTSCHPRPRDDPATTPSSSIPSTSAHNGGHTGPSLARRGIGFRDFLDIGGGWNMYYSAWPAVALPVQPAAWALANLYASILVQARGKWTASPPRHSFTVTYGGIRILMHCPQKPIPWLFVGNFAEKLLQITEAGWTGVYLVMLSQPETDVSIRVQLTVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FCX8</accession>
    <name>A0A8H6FCX8_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152511.1">
        <property type="nucleotide sequence ID" value="XM_037291076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FCX8"/>
    <dbReference type="GeneID" id="59328555"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="75"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="143" mass="16874" checksum="CC67C296CF1B7364" modified="2022-01-19" version="1">MYQGIQQLSHRLAGEPDDGIRRVWSRAQHRSHRTRPRFSNHPSLKLCTFQTIYTKAISEPVRCTPCCRRVERNIITEYEIENAQYETQVQLLTRELRAEMDADRRREMRGKISELVEELGDLKDRRNEEIAEVRFRQGVGASC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDD9</accession>
    <name>A0A8H6FDD9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_000587</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152961.1">
        <property type="nucleotide sequence ID" value="XM_037291526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDD9"/>
    <dbReference type="GeneID" id="59329006"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051858">
        <property type="entry name" value="WD_repeat_GAD-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017">
        <property type="entry name" value="GASTRULATION DEFECTIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="207"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="318"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="144"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="578"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="668"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6223744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="702" mass="76994" checksum="4BA4CA349F4F71DE" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDN9</accession>
    <name>A0A8H6FDN9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Survival Motor Neuron Gemin2-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HO133_010618</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153104.1">
        <property type="nucleotide sequence ID" value="XM_037301470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDN9"/>
    <dbReference type="GeneID" id="59339008"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22852">
        <property type="entry name" value="SMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22851">
        <property type="entry name" value="SMN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040424">
        <property type="entry name" value="Smn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047313">
        <property type="entry name" value="SMN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049481">
        <property type="entry name" value="SMN_G2-BD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39267:SF1">
        <property type="entry name" value="SURVIVAL MOTOR NEURON PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39267">
        <property type="entry name" value="SURVIVAL MOTOR NEURON-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20636">
        <property type="entry name" value="SMN_G2-BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20635">
        <property type="entry name" value="SMN_YG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival Motor Neuron Gemin2-binding" evidence="7">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="75"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF6224044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="175" mass="19998" checksum="C99B8165E6FB7F6D" modified="2022-01-19" version="1">MGRKRKQLSQAEVWDDTALLQSWDDALAEYKLYHSIYARGERVEDVIKEAEVEMQDAHMTEDDLRKAATDMQTNGIPSEELEDGELEEDKSAQTYYNGESTENFHGPSTIESKQKERLANVSTAVDQQEKPRMPNAVTNGVKDEALKNLMMSWYYAGYYTGLYEGQQQVQNTPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FDR0</accession>
    <name>A0A8H6FDR0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PLC-like phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_010678</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153164.1">
        <property type="nucleotide sequence ID" value="XM_037301530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FDR0"/>
    <dbReference type="GeneID" id="59339068"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF140">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26146">
        <property type="entry name" value="PI-PLC_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034024640" description="PLC-like phosphodiesterase" evidence="2">
        <location>
            <begin position="18"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="46097" checksum="EE9C05D2E3220324" modified="2022-01-19" version="1">MLLLLPFTLLLAIEVSAQVASDIQLSGSLTTGTSISNLGGDLLPTGSDVSYISYTTTSTLNGTTAIFQTTMAVANASSSSTSKQSTSTSLTLLQGSMRSSSTSSNGTMMGNSTASRTSTSAIPTNTQACNNYPEFCSRKYSNITYVGAHNSPFVLLNNAASNQQLGVIDQLDDGIRMFETETHYNATTSTVSCCHTSCNLLDVGTVQSYLTNITGWIKTHPYDVVTILLSNSDFIGVGNYSAPIVNSGLSNYAYVPPQIPMNISSWPTLSDMILMGKRAVIFMDYNANQTEVPYILDEFSQMWETPFSPTNQSFPCTQQRPPNLSRQEAENRMYMANHNLNTEISLAGTSLLVPTTALINQTNALNGTGSLGLMANNCAAAWPNSPNFLLVDYYNQGSSPGLACIAKGVLLEFARGNNGASIGPFCLAHFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FE62</accession>
    <name>A0A8H6FE62_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab GDP dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Rab GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153648.1">
        <property type="nucleotide sequence ID" value="XM_037300831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FE62"/>
    <dbReference type="GeneID" id="59338367"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000001">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF8">
        <property type="entry name" value="RAB GDP DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="749"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6224781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="801" mass="89930" checksum="0166805007DA449F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FF96</accession>
    <name>A0A8H6FF96_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154432.1">
        <property type="nucleotide sequence ID" value="XM_037300584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FF96"/>
    <dbReference type="GeneID" id="59338119"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="300" mass="34053" checksum="C38C59B1A443FDB3" modified="2022-01-19" version="1">MAIVHANIHAYLSKGGHAIAETKNNHRIIKTKPRLLRPPKVIDELVDRLMVQIKKDVALEKSLVVPPECWSRDSMTRPFKITAVSVLERVARGIPEAEWAQWQFLERLKKDWVYGAAVSPDTELSPQQTIPHQQMQIQDFHWENIQAAIDQLQKLEATLDGADPQELLRLKKNLTASDTMPFRLLDWDGNKLSGSVFRKTFGLHTSPSSEDDLQRKQGERRRQENQQLETPLKRKSTDEPQQQNRQKRSKTTPVKSSETAEPSVSDAITLASPASNWQTIKGQFGNAPIVKRQTPIAVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFF4</accession>
    <name>A0A8H6FFF4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154583.1">
        <property type="nucleotide sequence ID" value="XM_037300735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFF4"/>
    <dbReference type="GeneID" id="59338271"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6225874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="312" mass="34262" checksum="FAA11E54025EEACC" modified="2022-01-19" version="1">MATAPSQLQNRLALVSSLYGPGTITCWYLTALSVLVSWTLHPRKRKSGSIDVDLIAVLTLPAVAAGHLVAQARKFSNQDNHAQTTSSADWKYLQSIAAIEAPFNVTETFMAISVILFIVAAWMFCIRRAIFVALVGLLCFAVECYIHFSRFMDLGVRYEPRVSAGDYPAFSRLFVADFAGLVVAILVTLSICGLISAAIAFSMLVPPRTPSSRSRQDIERVNEAALREGISFRFGSSAAEGTPSTQTTHGTPLAEKSEDLHLRAITMVTTLFLPLTFILSLLPSYWHSTMYYDVVLADARPCRKSILQDVEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFM6</accession>
    <name>A0A8H6FFM6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cellulase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_009431</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 45 (cellulase K) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155118.1">
        <property type="nucleotide sequence ID" value="XM_037300292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFM6"/>
    <dbReference type="GeneID" id="59337826"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052288">
        <property type="entry name" value="GH45_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000334">
        <property type="entry name" value="Glyco_hydro_45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39730">
        <property type="entry name" value="ENDOGLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39730:SF1">
        <property type="entry name" value="ENDOGLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02015">
        <property type="entry name" value="Glyco_hydro_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolases family 45 active site" evidence="9">
        <location>
            <begin position="5"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6226565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="216" mass="22643" checksum="AD9099DAD4990F51" modified="2022-01-19" version="1">MSGITSAVNTCDISDSPLADASAQSGCTGGTAYMCSDQSPWSINDDLAYGFAAVSASNPNCSSCYQLTFTSTVLSGKKMIVQATNTGDDVSSTQFDLAIPGGGFGQYDGCSTEWETTSAVWGARYGGPSSNTCSSLPSALQPGCGFRWDWMQGADNPTVDWEVVTCPAELVGRSGCSGLLSSRTRTFTQSPHLFPTCPSRQQAARGIPNREADHQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FFT0</accession>
    <name>A0A8H6FFT0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_009130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154817.1">
        <property type="nucleotide sequence ID" value="XM_037299991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FFT0"/>
    <dbReference type="GeneID" id="59337525"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6226264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="501" mass="57205" checksum="E5250195A4FF5FB9" modified="2022-01-19" version="1">MMSQNTEDVLMTDVQDKSSDAKTTRFPTKRPSPPTPSGHEEPQETERSKYPCSKAAENIDGELENQLSEAREGAKDFEHRFNEARDLNTNLQVAFTISQNESIELKKKIKTLQNESTELKKKYKTLQNGHDVIHEQRTAQLLSDVEGRRKQADSNTQIALLKVQELSEELQKCKDELFNLQPPNPVADTHISAEWDALCSSITTWIDDQSGGVEDLRTRFRELNSNKKCSKALAKYWGEDRQLIANHYSQSSNILDEILRYNIHCFLEAEVFDDRVYMVGLSTSEAKLLLKIEREMAKLEPRRDPSYIGLWRSEALAALSSTAGFSTFLEKDTQKIRQEASEILEPLLQRRARGAMSAFDRDIIMPAIRLASTLRQSTAKYHFVFRVVSNKHEEKPPALQKPGNRLLLYKSDLEDIDVLDVGSRITLKKGRNYEEASDGSIAESILVVHPALYRETPTERILVSRQLVLAKLLKPPQRRGQARDVEENRGWFDKLGLKFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FG21</accession>
    <name>A0A8H6FG21_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_008426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155293.1">
        <property type="nucleotide sequence ID" value="XM_037299292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FG21"/>
    <dbReference type="GeneID" id="59336822"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF2">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="42166" checksum="36F6E66440FDC8DD" modified="2022-01-19" version="1">MGNLLTKPDREARVTSASAGDFAITTPGTFDEDPATVNKNRICLKPALEAITTTPLSEVPSPPRLKHRAPVRPPNSPETDRWSFQGSSRSGSAHSPLPHTKRYDTPSDSGSYTDKYADMSSDSDFFQLIVGPAVDIFVGPEEVQFTLPRKLLYYHSRFAERAIEGFFREASEKALRLPQDDPEVFYWLVQWMLQGNLEMVEQLRQKYQWKNGRQDWREYACRLLCRLWILGDKILHEPESSRRWPGISFAWSIRWGLHDLFCGAQDDNLRTPLIPDIVIEVWQNTMPDIEEPGKHRFGPRDRSLRQILFDELCDSHFHKTGVFDCSEYSKCFMLDGGKFAADVLAYLMRFANMDFDERNVEMQWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FHJ4</accession>
    <name>A0A8H6FHJ4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_007762</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000004">
        <property type="protein sequence ID" value="KAF6228034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155968.1">
        <property type="nucleotide sequence ID" value="XM_037298632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FHJ4"/>
    <dbReference type="GeneID" id="59336159"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6228034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="99" mass="11126" checksum="1F102B344266739C" modified="2022-01-19" version="1">MDSDQEETRPESSLTIQHYQAQIHGLNTTIQNLNTTAKIQALTIKEETVTPAEPLDSDVDEQPIKLKVTKTGTPVPTILVIGNNKKYPNVPYFYSEKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FJV6</accession>
    <name>A0A8H6FJV6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6229835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157092.1">
        <property type="nucleotide sequence ID" value="XM_037295091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FJV6"/>
    <dbReference type="GeneID" id="59332581"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6229835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="138" mass="14708" checksum="9A7988BFD9096930" modified="2022-01-19" version="1">MGPVDRSQYDSLTTVPGEALVTLVGIYKRTSYENSDVASLAISNDCAAQGGQDAVLATVACNITVTGYKAGSSSPYTTQNFQFTSEEPVDVMSPLAFGTFLVKFQNLQDVTLAVEPPLLTSAHGSCLTKTEENDDVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK75</accession>
    <name>A0A8H6FK75_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157323.1">
        <property type="nucleotide sequence ID" value="XM_037295322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK75"/>
    <dbReference type="GeneID" id="59332813"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF16">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="56896" checksum="7A027742774EBDA3" modified="2022-01-19" version="1">MQNLPLRPGPATTSPPPDIEIWKAVPSDVTPMADLFLDKYGGDKATRLMYTKSEVWPTILDTIESYIDFEREVQLMIAMDIDTRIPVGLISVGVVPDGVHVDDYTGSELSVHANWAMRAERASARGEDPRRLTDARLRLSDVVDNRSRAGQDRYIGRSHLVLNTFAADLKGPTGRIYQSLLTWVINFAQIRNWPIWAQFPARHVGPLQTRLHAIFDVPQKKYTATHQLFTMPRGTESGGDSPAELDFTELDELFIMTRDAKRDDVGAMANIFIKSFKDDKTAQLLYPRDEIWPVVVEMLRHYLDDDYTHVIVAEDRYTDTTVGWTSVSLVASDQEDHFKFCDSTVWAGRRLLHLGRSEGPLHVDEVRRAGLITQLSRRNRDGQKREIDGQHLVINTFAIHPDVFEEEIPEIAYKLMDDTRDLAKGEGLPLWAQFPQNSLGNLEELFQEIGFAEVGSFELNLTGYTNEEHRRRRNWGWQKWTQWVLEIGNWECGRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FK86</accession>
    <name>A0A8H6FK86_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Calnexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_004366</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the calreticulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157285.1">
        <property type="nucleotide sequence ID" value="XM_037295284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FK86"/>
    <dbReference type="GeneID" id="59332774"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.250.10:FF:000001">
        <property type="entry name" value="Calnexin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000011">
        <property type="entry name" value="Probable calnexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF1">
        <property type="entry name" value="CALNEXIN 14D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00803">
        <property type="entry name" value="CALRETICULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00805">
        <property type="entry name" value="CALRETICULIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034252050" description="Calnexin" evidence="9">
        <location>
            <begin position="24"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="507"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="247"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="541"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="260"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="541"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="151"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601580-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6230028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="64505" checksum="816CFD3C39CF66A9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKC3</accession>
    <name>A0A8H6FKC3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">18S rRNA biogenesis protein RCL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_004475</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157393.1">
        <property type="nucleotide sequence ID" value="XM_037295392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKC3"/>
    <dbReference type="GeneID" id="59332883"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000004">
        <property type="entry name" value="18S rRNA biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016443">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03400">
        <property type="entry name" value="18S_RNA_Rcl1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="5">
        <location>
            <begin position="16"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="6">
        <location>
            <begin position="206"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6230136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="41990" checksum="B19E48B0F18C1F0A" modified="2022-01-19" version="1">MASTPQAPVYLTTQKSLAHRLILSTLTGRAVHFKQIRPSSLTHTGLAPHEISFLRLLEAVTNGSQFVISVTGTELLYKPGLITGSAPGSGASAGVIKHELPAECTRGISYFLVPLCLLAPFSKAPINVLFTGPGVITSATPSGDLSADSVRTAILPLYAQFGITDNIELRILRRSNVSTGGKGGAGEVQLVFGHQVRLPKTVHIMNPGRVKKIRGVAYCTGVSASNNARMIEEARGVLNPMTADTYIFSDVSSAPYPKSRDKSNTNQNRKTGVGFGLSLVAESSTGSLYSADLASPPAGGVPPEDIGRQCAYQLLESISFGGAVSLAAAPTVLTLMAMGSEDVGRLQLGKDVLSSEPIIGLARDLKAFGASGWGVREPITGLDGDVVVSVVGRGVGNVGRKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKH4</accession>
    <name>A0A8H6FKH4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157468.1">
        <property type="nucleotide sequence ID" value="XM_037295467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKH4"/>
    <dbReference type="GeneID" id="59332958"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="203" mass="23430" checksum="3F866C256C044AD0" modified="2022-01-19" version="1">MVSLMEATADRSCLHSVQMILPKDETRAKVKAICNQNLRIGKVVDAYQQTLRDFEVALWELHDPSAWSIAQLKNPKRLSIRLDHYHTRFPGIERQFWESSPRSTVWNNLASTPEKTALGCLRSLYLERAGITDYQLATALKSNPMLTELRFRKCLTLTDRTFKFFAESNVGRHIETLCLIKSYRDELNDGLFEHTAKLTNLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKJ2</accession>
    <name>A0A8H6FKJ2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157469.1">
        <property type="nucleotide sequence ID" value="XM_037295468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKJ2"/>
    <dbReference type="GeneID" id="59332959"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="564" mass="63225" checksum="8FEDF8CDFA72824E" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKP2</accession>
    <name>A0A8H6FKP2_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_004642</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157559.1">
        <property type="nucleotide sequence ID" value="XM_037295558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKP2"/>
    <dbReference type="GeneID" id="59333049"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6230302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="173" mass="19158" checksum="AB1C87F7E5AB8172" modified="2022-01-19" version="1">MSSTPSIKALLSIAVQLAESLSERIPNLGSLQRDCKQTAMNLRTWRTNLPSEMEFLLKPTGQLIWTTHELGKPPSLDTVSRTYEAEQKIKSASPSIKKFMDDLGVWQKPKKGHAPLAVLFDQTSRSDKVFTFTEKAVNGHMGACSNGLMGLTTRIDRARKRHVGLRLLAGKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FKU3</accession>
    <name>A0A8H6FKU3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157600.1">
        <property type="nucleotide sequence ID" value="XM_037295599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FKU3"/>
    <dbReference type="GeneID" id="59333090"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="440" mass="49578" checksum="4D91A516722324FE" modified="2022-01-19" version="1">MLSSFSTRPAHHQVPSKLIIRSTSLAAVVIVSLCSILYLKRSFSTYTVKTKLERLLPGVSVNTEIKLHWNDSPRRLIVFGDSWSDNGQYPVNPPTRELTPTRDDAQGKIWTDWLCSSISCTHHDNFARSLPHVWDSGYSGAVVDSNLLNSTLSNTTLSNSTFITDIEWAEPLADLKAQVQHWLAFEKKQYANSRVSEVERSGTVFIVWFSLWDLWYYSEKTLAEAEAAVAKTMDALFEQLDVIAENWPSEMKLIIPEAIDTTFLPGWHTMRTGPRGSDPLGEKQRSAVLLVEQWNRGLDMRAVRWRGGSMYIYNTNEWLLDQVREQQLSTAHLSDANGMGANQSPWNNVRSGCVASNYKAYDVLGEDPTAGRCSDPTTYLFWDDMHLGPEAMKMIGQGIAQDIAANRATSWFAHAVEESYEDEDEEAPLPQDPHPEDSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6FL62</accession>
    <name>A0A8H6FL62_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157774.1">
        <property type="nucleotide sequence ID" value="XM_037295773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6FL62"/>
    <dbReference type="GeneID" id="59333267"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF11">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="47"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="636" mass="71959" checksum="4F89A39D1BB3448A" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6KY76</accession>
    <name>A0A8H6KY76_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_007133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000003">
        <property type="protein sequence ID" value="KAF6229019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037156661.1">
        <property type="nucleotide sequence ID" value="XM_037298026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6KY76"/>
    <dbReference type="GeneID" id="59335533"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000002">
        <property type="entry name" value="10 kDa chaperonin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF0">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6229019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="104" mass="11258" checksum="29224EC8842E3594" modified="2022-01-19" version="1">MTTALKSIKSLAPLLDRILVQRIKAETKTASGIYLPESSVKELNEARVLAVGPGGLDKEGKRVTPSVTPGDKVLIPQYGGSPVKVGEEEYSLFRDHELLAKINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6L044</accession>
    <name>A0A8H6L044_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rxt3-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_004866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000002">
        <property type="protein sequence ID" value="KAF6230522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037157779.1">
        <property type="nucleotide sequence ID" value="XM_037295778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6L044"/>
    <dbReference type="GeneID" id="59333272"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013951">
        <property type="entry name" value="Rxt3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08642">
        <property type="entry name" value="Rxt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="622"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="985"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="639"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="682"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="696"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="707"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="735"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="751"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="985"/>
            <end position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6230522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1031" mass="113635" checksum="71C4992AE1A2D616" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C545</accession>
    <name>A0A8H6C545_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Carboxypeptidase</fullName>
            <ecNumber evidence="13">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_006677</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Vacuolar carboxypeptidase involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Release of a C-terminal amino acid with broad specificity.</text>
            <dbReference type="EC" id="3.4.16.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="13"/>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147010.1">
        <property type="nucleotide sequence ID" value="XM_037297574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C545"/>
    <dbReference type="GeneID" id="59335078"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.410:FF:000001">
        <property type="entry name" value="Carboxypeptidase Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008442">
        <property type="entry name" value="Propeptide_carboxypepY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05388">
        <property type="entry name" value="Carbpep_Y_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034288603" description="Carboxypeptidase" evidence="14">
        <location>
            <begin position="16"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Propeptide carboxypeptidase Y" evidence="15">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6217575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="540" mass="60694" checksum="F60C83D7B7253ECB" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6H9</accession>
    <name>A0A8H6C6H9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000025">
        <property type="protein sequence ID" value="KAF6217780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147215.1">
        <property type="nucleotide sequence ID" value="XM_037297505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6H9"/>
    <dbReference type="GeneID" id="59335008"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="225" mass="24529" checksum="4AA762BD8019124C" modified="2022-01-19" version="1">MSDQAAKKSKPMPPLGAFAKKLDSQVDRVESGASKAEQKRAQEQHEGDTTKEYGLGAKVKPMPKKARARVSGTHSKRVESLLQQYEEDMAEKEKGDSTEYSSLGDGGKRLPRKRDFQESKLSHPDEYESESWRVEGSGKNLEGPGGTGGQLGAEVTSSRPRRTPASKPDSQVGSGRVRSEGPVMGAQRPRTGNTGNSQEPTESWASRNEGDGFESFEQWDLGDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C6K6</accession>
    <name>A0A8H6C6K6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_006708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000026">
        <property type="protein sequence ID" value="KAF6217606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147041.1">
        <property type="nucleotide sequence ID" value="XM_037297605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C6K6"/>
    <dbReference type="GeneID" id="59335109"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="628"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1000"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1063"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1083"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6217606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1111" mass="125342" checksum="0EEB9C402C01D408" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C759</accession>
    <name>A0A8H6C759_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_006298</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000024">
        <property type="protein sequence ID" value="KAF6217886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147321.1">
        <property type="nucleotide sequence ID" value="XM_037297196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C759"/>
    <dbReference type="GeneID" id="59334699"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071021">
        <property type="term" value="C:U2-type post-spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.940.10:FF:000008">
        <property type="entry name" value="Related to potassium channel regulatory factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.110">
        <property type="entry name" value="Pre-mRNA processing factor 4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004098">
        <property type="entry name" value="Prp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036285">
        <property type="entry name" value="PRP4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039979">
        <property type="entry name" value="PRPF18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007:SF19">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02840">
        <property type="entry name" value="Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47938">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158230">
        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="10">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Prp18" evidence="9">
        <location>
            <begin position="215"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="52"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6217886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="365" mass="41123" checksum="8DA52D3B66842EB7" modified="2022-01-19" version="1">MDFASLMSAEISKSSSKPSSSPTPTPTEPTKKYLKRAELEAQRQQAYVDEQAAIRRDRESKLLKKRKLDEEDAARNAERDGKRRRLAEESRIRREEEEAKRERARRKRLGLPELPEKTTAEDGTAVRGGEPDMPDPDLVAKLRALAEPTLLFGETHVQRLRRYRRLVAPGPAPPAMTSGPIPTTLSLLPEAEMKVPPTLPKDVDGRTFLFRQLASYFTMVLTEWSRALGRRDGGVKESQQGRQAHGAYAQSRANMAPLFRKFEAGQLEEGILAPMVEIVVAAQERRYVDANDGYLRLSIGKAAWPIGVTMVGIHERSAREKLHESDAAAHIMSDEVTRKFLQSIKRCLSFAQTRWPPEDLMQLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C7V2</accession>
    <name>A0A8H6C7V2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 subunit I</fullName>
            <shortName evidence="7">eIF3i</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 39 kDa subunit homolog</fullName>
            <shortName evidence="7">eIF-3 39 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TIF34</name>
        <name evidence="9" type="ORF">HO133_005851</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eIF-3 subunit I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C7V2"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000127">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03008">
        <property type="entry name" value="eIF3i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6218502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="338" mass="37326" checksum="A2A0BAABCFBC60E9" modified="2022-01-19" version="1">MRPILLQGHERSLTQIKYTKDGDILFSCAKDQVICAWFSANGERLGTYHGHQGALWTIDIDPSTTLLASGAADNTVRLWDIRTGKCIKVWDFNTAVKRVEFNDDGTKLLAVTEKRMGYLGTIVVLDVKVDVEAEQSDERSLVITCDESKATVAGWSYLSRYIIAGHEDGTVSQYDAKSGDLLINEPVHEDGGQVTDLQMSSDRTYFITASKDKTAKLISARDLTLLKTYPSDTPLNTAAITPKKPFVILGGGQAAMDVTTTSSRQGKFEARFYHKVFEDEIGRVKGHFGPLNTVAVDPLGRGYASGGEDGYVRVHAFDRGYFDFGYEVEREMGLVGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C849</accession>
    <name>A0A8H6C849_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2 14">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HO133_005713</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2 14"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147801.1">
        <property type="nucleotide sequence ID" value="XM_037296620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C849"/>
    <dbReference type="GeneID" id="59334118"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07857">
        <property type="entry name" value="STKc_MPK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000013">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000157">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="23"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="376"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF6218366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="415" mass="47616" checksum="E5D6557E77B41C66" modified="2022-01-19" version="1">MADLQGRKVFKVFNQDFIVDERYNVTKELGQGAYGIVCAATNNHTGEGVAIKKVTNVFSKKILAKRALREIKLLQHFRGHRNITCLYDMDIPRPDNFNETYLYEELMECDLAAIIRSGQPLTDAHFQSFIYQILCGLKYIHSANVLHRDLKPGNLLVNADCELKICDFGLARGFSMDPEENAGYMTEYVATRWYRAPEIMLSFQSYTKAIDVWSIGCILAELLGGRPFFKGRDYVDQLNQILHYLGTPNEETLSRIGSPRAQEYVRNLPYMPKVPFLRLFSNANPDALDLLDKMLAFDPSARISVEEALEHRYLHIWHDASDEPSCATTFDFHFEVVEDVPEMRKMILAEVARFRQQVRVQQPQQQMSGQINVPIPENANQWSHEDPRPQEAAHSQQMHGMELEQELQGGLDAMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C8D3</accession>
    <name>A0A8H6C8D3_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_005878</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JACCJB010000022">
        <property type="protein sequence ID" value="KAF6218529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037147964.1">
        <property type="nucleotide sequence ID" value="XM_037296783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C8D3"/>
    <dbReference type="GeneID" id="59334283"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="62"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="430"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="483"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="615"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="616"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6218529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="735" mass="83344" checksum="078181863DA5417D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9C8</accession>
    <name>A0A8H6C9C8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_005157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148767.1">
        <property type="nucleotide sequence ID" value="XM_037296069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9C8"/>
    <dbReference type="GeneID" id="59333563"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="116"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6219332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="334" mass="37367" checksum="D7112272BA2C61EA" modified="2022-01-19" version="1">MASELKTDPKYDDYEYPTTSPNVQSGHPGHTTPEQDAQVFQLRTQLEQAGYTERLDTLCMVSIVDVVFRPSASLTLNSLGSYAHESSTSTMQSKYCEKWRKDFGVDELVRTFDYIEKPKVFEYYPQYYHRTDKEGRPVYIEQLGKIDLAAMYRITTAERMLQNLVVEYEKLADPRLPACSRKSGQLLETCCTIMDLKGVGISKVSSVYGYVKQASAISQNYYPERLGKLYLINAPWGFSSVFSVVKGFLDPVTVQKIHVLGSGYQTELLGQVPKQNLPKLFGGTCACEGGCALSDEGPWQDSEWTKAPKWARTEGESKGSKPTSTTSAVQPQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6C9G0</accession>
    <name>A0A8H6C9G0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prefoldin subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HO133_004983</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000020">
        <property type="protein sequence ID" value="KAF6219158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037148593.1">
        <property type="nucleotide sequence ID" value="XM_037295895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6C9G0"/>
    <dbReference type="GeneID" id="59333389"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23161">
        <property type="entry name" value="Prefoldin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000003">
        <property type="entry name" value="Prefoldin subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="19"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF6219158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15733" checksum="D7797E66E36D1885" modified="2022-01-19" version="1">MDIEISTLPSKTRCPTYDMAEDQKKLQELSDAYQKIQTDLQTTVSARQRLESQQQENKGVQKEFSTLPSTSAIYKLVGPVLLKQERSEAVMAVEGRLDFIDREIKRLEKQIQETQTESESKKMEVYQLQTQMQGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CBR4</accession>
    <name>A0A8H6CBR4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_003461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000017">
        <property type="protein sequence ID" value="KAF6220329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037149764.1">
        <property type="nucleotide sequence ID" value="XM_037294383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CBR4"/>
    <dbReference type="GeneID" id="59331872"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034742270" evidence="1">
        <location>
            <begin position="20"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6220329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="318" mass="34655" checksum="5791F9916E55635F" modified="2022-01-19" version="1">MSSLTGLVFLHIFIATVHGLAPPATNRAISVPSSSTSLTLDLLNLTSSANNLQLVNTTANENIKCDGKYWGYHLNKASCEEAWDKMPTDNQFVSFGARRMGHFERPLPYRYLSDDGLCAIDIDHMIKVVLDTATNHEISAAAKSILDKCVLRDEPKKQGNTPIGGYRNYIGRKKGLELEDETICTKLLGTMPINRSSYVFIREKTSGPRSVIIPWGGRKISSGTHASYPASLRLLQKASSSPCSLATIVLLTVPGTCIATIDFEEGDTDKSSWYEMWMAAVAVNEMCVTKGKAGAAFGLGKSILSTLSKNDCRRAEVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CCL4</accession>
    <name>A0A8H6CCL4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_002511</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000015">
        <property type="protein sequence ID" value="KAF6220831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037150266.1">
        <property type="nucleotide sequence ID" value="XM_037293437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CCL4"/>
    <dbReference type="GeneID" id="59330924"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF123">
        <property type="entry name" value="GMC OXIDOREDUCTASE (AFU_ORTHOLOGUE AFUA_2G01770)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="5">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="261"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="495"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="533"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="534"/>
            <end position="535"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6220831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="552" mass="59019" checksum="DF264D4FBF7054D7" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDU7</accession>
    <name>A0A8H6CDU7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chromatin remodeling complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_001625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151092.1">
        <property type="nucleotide sequence ID" value="XM_037292553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDU7"/>
    <dbReference type="GeneID" id="59330039"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="380"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="438"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6221657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="682" mass="73733" checksum="1F59D5E7FF225F40" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CDZ3</accession>
    <name>A0A8H6CDZ3_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151208.1">
        <property type="nucleotide sequence ID" value="XM_037292669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CDZ3"/>
    <dbReference type="GeneID" id="59330155"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6221773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="178" mass="19379" checksum="279A76DDA7717650" modified="2022-01-19" version="1">MDIANKLFSLTLGVEPNKATLQGLASTLNSAVLSLADKQKALEQFADTAEAQKFVSQAPIPQQQFTQPQIQYIQAAPTPQAAQARRAKILRDPVVWQISEKQMKELQWKRKQLLQAQKAELERKKTARAKRPPPPKLGASKNGVNKTQQNKAPAKPQAPPAKAPAAPKAPAKAPAKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CED7</accession>
    <name>A0A8H6CED7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HO133_001699</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, involved in the regulation of DNA damage response mechanism. Required for the control of telomere length and genome stability.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associates with DNA double-strand breaks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151166.1">
        <property type="nucleotide sequence ID" value="XM_037292627.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59330113"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00892">
        <property type="entry name" value="PIKKc_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000031">
        <property type="entry name" value="Phosphatidyl inositol 3-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056802">
        <property type="entry name" value="ATR-like_M-HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012993">
        <property type="entry name" value="UME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF125">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MEC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25030">
        <property type="entry name" value="M-HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08064">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00802">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="17">
        <location>
            <begin position="1186"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="16">
        <location>
            <begin position="1877"/>
            <end position="2196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF6221731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2220" mass="248837" checksum="B956C76EF30DFC38" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CEU9</accession>
    <name>A0A8H6CEU9_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_001874</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000013">
        <property type="protein sequence ID" value="KAF6221906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151341.1">
        <property type="nucleotide sequence ID" value="XM_037292802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CEU9"/>
    <dbReference type="GeneID" id="59330288"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="65"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6221906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="662" mass="70856" checksum="964AD4D361B73F69" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CF73</accession>
    <name>A0A8H6CF73_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_001488</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. CAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151837.1">
        <property type="nucleotide sequence ID" value="XM_037292418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CF73"/>
    <dbReference type="GeneID" id="59329904"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000509">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF171">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="48"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6222402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="486" mass="53626" checksum="06C8E5E12A90F00C" modified="2022-01-19" version="1">MTDGSDERSPLLQNGHAEDQQNDPEIIDFSKEDDENPRNWPRRRKFVNVAIIAFMAILSPLASSMFTPGISEIAVDLNTSDSTVVGATTGFVVFLGIGPLILAPLSETFGRRKLYLVCFSIFALLQIPTALSPNVEALIGVRTISGFFGSVGIANGGGTISDMFHPSQRAGVFGWYLLGPLLGPTLGPLFGGIIVQYLGWRWIFWILTIVCSFNTVVGFFFLKESYAPVILEARKKTYEDSSESGSKSYRMAGQDDRPLRSKLANSMARPLRILFMQPVVFTMAVYQAILFGTTYSLYTNFQAIYGELYGFDTTTVGLMYLGPGLGFLTAVWFIVPKIDTIYNYLARKNNVEAKPEYRLPLANIGAVFIPTSLFVFAWTVQYKVHFVVPLISTVFFGLGQITILNTCQNYYIDSFQQYAASAIAAGAVFRSIVGGILPLGASKLFESLGYGWGISIFAFLSLALSPAPLLFFYYGEGVRERFAIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFB0</accession>
    <name>A0A8H6CFB0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_001505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151854.1">
        <property type="nucleotide sequence ID" value="XM_037292435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFB0"/>
    <dbReference type="GeneID" id="59329921"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6222419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="145" mass="16672" checksum="F234BF83BAB853CE" modified="2022-01-19" version="1">MAHELLPFLDKEEQKAEAKSKEPKDLEAFMNEERSAIANNVAPADEAYSQFHQKSPPLTTLCPTHLKLQDAVSPLRFDGQLVKRRNRLPADMAFAEDDYFSLFRPEPPADDKSRMKDEAKGQQHRWTVEKELQGTMNMRSIEKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFL0</accession>
    <name>A0A8H6CFL0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_001365</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000012">
        <property type="protein sequence ID" value="KAF6222279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037151714.1">
        <property type="nucleotide sequence ID" value="XM_037292295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFL0"/>
    <dbReference type="GeneID" id="59329781"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46972:SF1">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46972">
        <property type="entry name" value="MONOOXYGENASE ASQM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="7"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6222279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="430" mass="48097" checksum="932C4DF43F7EFDB1" modified="2022-01-19" version="1">MASAPKIAIIGAGPAGLTLARLLHVSEVTVDLTLYDLDASPTSRPDRGGTLDLHADTGLAALRKCDLWDSFRKYARYDGQEFIIVDKNATELVHRRGGERAGPSDRPEIDRERLKEILLESVPEEWVRWGRHLREVTEDGKLRFDGREQLEGPFDLIVGADGAWSKVRGRLNEIEPAYSGVSGYETEIMEPARTCPHVDKMVGRGSIIGSSDAKFLNAQRMGNDSLKVRSWYACPEGEAKETLDKYGKKGTLEKILERYAGWAPEMTELFRQGDLDELKHWTLYELPVGYKWDHKQGFTLIGDAASLATPFSGEGVNKAMKDSLELAELIEKSQDPNDDLTLDQAVLLYEQLMFPRAEKVQARTMNNKQIAFGPEAPLGLMTGMLKMKAGDSPSILVRMLGTAPVVAAVYGYFWIRAQIGWAVRRLWRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CFZ0</accession>
    <name>A0A8H6CFZ0_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000797</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152095.1">
        <property type="nucleotide sequence ID" value="XM_037291733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CFZ0"/>
    <dbReference type="GeneID" id="59329215"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034635669" evidence="2">
        <location>
            <begin position="22"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="645" mass="71826" checksum="4F0706836212190F" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGD2</accession>
    <name>A0A8H6CGD2_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7918 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_000794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6222746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152092.1">
        <property type="nucleotide sequence ID" value="XM_037291730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGD2"/>
    <dbReference type="GeneID" id="59329212"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057678">
        <property type="entry name" value="DUF7918"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR EAF N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25534">
        <property type="entry name" value="DUF7918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="6"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6222746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="321" mass="35834" checksum="92709A6FD3AE6294" modified="2022-01-19" version="1">MDHFQGITAQIISNGQVLDFYDDPDAPEIPESRARHHYVEAVAGSTFQVKVNLTPQFNSYRMKAEDAVKISVVPDGNIGGGGRAVKYTKKLLQESFSRVYRCSHTFTGPKHFCKEIGQWMRSDYSFGNLVLKETPDPGFSVKQAEDLGKIQITIQRVKMEKRQVAYVPSTIPIETINEVPEKALKGRSIETTVRLANARPTSASVHSHISKSISGEAGKPVVFNIYYRSRRALQMIGCIPRSPSPESQSPDDDATAPEHVLQEVRDLRARLALLERGLNVKSESSSAATGVKREREEEGNKGSRQRRRTTVKIEHVDLTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CGP4</accession>
    <name>A0A8H6CGP4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_001097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000011">
        <property type="protein sequence ID" value="KAF6223045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152391.1">
        <property type="nucleotide sequence ID" value="XM_037292029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CGP4"/>
    <dbReference type="GeneID" id="59329515"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="84" mass="9553" checksum="76C0E16DB564151F" modified="2022-01-19" version="1">MAGTFTTYAEMLEAMKGLHLAKSVMDLLGPAGKHVKTWAQLDSFMHSQQLNKRTFLTPQRIADLLLQRLRDFYLANEARKGSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CH50</accession>
    <name>A0A8H6CH50_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HO133_000251</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0220 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152626.1">
        <property type="nucleotide sequence ID" value="XM_037291191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CH50"/>
    <dbReference type="GeneID" id="59328670"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007919">
        <property type="entry name" value="UPF0220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13180">
        <property type="entry name" value="SMALL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05255">
        <property type="entry name" value="UPF0220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF6223409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="171" mass="18676" checksum="840AB74E13F62725" modified="2022-01-19" version="1">MTSSRLFRFPKPEWVNSPNTRTAGVYLAGALFSLGFFFFIDASTYSHSSKNGSNVHIKFVDWIPGICSALGMLVINSIEKTRLSADSYSYSGTGVAWKARLVLFLGFALMAGGLAGSVAVLVLKYIVPDYPFPTLYFGVANVVANGLIMLSSVVLWVSQNMEDDYTYNLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHA6</accession>
    <name>A0A8H6CHA6_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_000051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152426.1">
        <property type="nucleotide sequence ID" value="XM_037290991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHA6"/>
    <dbReference type="GeneID" id="59328470"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6223209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="153" mass="16865" checksum="3FFEFB67C8BB77AE" modified="2022-01-19" version="1">MSLLDPRLPSNSRLVLCHAHSAWAKYSGDVSGVYDKAENTFTSTRLLEHTLLKDVGTERAIANATITLFRERTQLWTLVENEGRYLRVDMEFAGSPARSARVCGKMVTGDDVLGAVQLSREEELRLGIGGRSGLGNRIDMSGAIILDVPQFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHM7</accession>
    <name>A0A8H6CHM7_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_000223</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152598.1">
        <property type="nucleotide sequence ID" value="XM_037291163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHM7"/>
    <dbReference type="GeneID" id="59328642"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF692">
        <property type="entry name" value="TRANSMEMBRANE AMINO ACID TRANSPORTER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="519"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="577"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="600"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="636"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="259"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="112"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6223381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="669" mass="73451" checksum="287369874F9F6BAA" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHN8</accession>
    <name>A0A8H6CHN8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">alcohol O-acetyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.84</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Alcohol O-acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_000246</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Displays enzymatic activity both for medium-chain fatty acid (MCFA) ethyl ester synthesis and hydrolysis (esterase activity). MCFA are toxic for yeast and this enzyme could thus be involved in their detoxification by esterification.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an aliphatic alcohol + acetyl-CoA = an acetyl ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:17229"/>
            <dbReference type="ChEBI" id="CHEBI:2571"/>
            <dbReference type="ChEBI" id="CHEBI:47622"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.84" evidence="6"/>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152621.1">
        <property type="nucleotide sequence ID" value="XM_037291186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CHN8"/>
    <dbReference type="GeneID" id="59328665"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004026">
        <property type="term" value="F:alcohol O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000137">
        <property type="entry name" value="EEB1p Acyl-coenzymeA:ethanol O-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF63">
        <property type="entry name" value="ALPHA_BETA HYDROLASE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="9">
        <location>
            <begin position="129"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6223404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="434" mass="48913" checksum="5D30F2B5D89B05AC" modified="2022-01-19" version="1">MDFWRLFGYAKTSFYHHDSPLFLPTKNTKTGEQPTNLLDLCKHVTPVCRLNPLLFNGHLQTFWTAVKSQDIPIYYKRRLFSAEEPQFAGTFAVDFAVNPYEGTDESLPPRTTYYNDGEFEDIGSMDTKPMLVTLHGLSGGSHEIYLRHVLRPIVDAGWEACVVNSRGCAKSKITTGVLYNARATWDVRQVVKWLRRTFPNRPLFGIGYSLGANILVNYLGEEGESCMLNAAVICSNPWNLEAGNLALQRTWLGSEVYSKTMGTSMKKLFEDHVDAISKNPRVDIEKVRNITYLYEFDRELQGPTWGYPTEGAYYRDASSCDSLMAVRIPLFAIHAEDDPIAVKEAIPFREFQQSPFGVLCTTSLGGHLSWFENGGGRWFAKPASQFLIQFFENIDLDKLEKKPGEKAGNGIGLGGKALASFNPMRRKLQIDVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CHQ0</accession>
    <name>A0A8H6CHQ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">SNF2 family helicase/ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_000471</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152845.1">
        <property type="nucleotide sequence ID" value="XM_037291410.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59328890"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18007">
        <property type="entry name" value="DEXHc_ATRX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044574">
        <property type="entry name" value="ARIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056026">
        <property type="entry name" value="DUF7607"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45797:SF1">
        <property type="entry name" value="HELICASE ARIP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45797">
        <property type="entry name" value="RAD54-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24580">
        <property type="entry name" value="DUF7607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="1210"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="1617"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="146"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="274"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="756"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="835"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="905"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1103"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1550"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1856"/>
            <end position="2052"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="687"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="274"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="756"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="860"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1129"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1920"/>
            <end position="1945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1955"/>
            <end position="1967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1974"/>
            <end position="1984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1988"/>
            <end position="2007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="2012"/>
            <end position="2031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6223628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2072" mass="232153" checksum="B7252E5A736644C9" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI13</accession>
    <name>A0A8H6CI13_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_000459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000010">
        <property type="protein sequence ID" value="KAF6223616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037152833.1">
        <property type="nucleotide sequence ID" value="XM_037291398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI13"/>
    <dbReference type="GeneID" id="59328878"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF10">
        <property type="entry name" value="ABC TRANSMEMBRANE TYPE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6223616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="37242" checksum="44CB7538D147434F" modified="2022-01-19" version="1">MDQHIEVDDQEPASKDDEPTDEGIPRTSSEPEHSEVSKHSTRSITRSLVEEHFENGRRYCSEDYYMPNDEPEQTRLAIAHQAYMPALDDQLTWGLIPRSAKRILDIGTGTGDWATAIAERFPNAEIIATDITNAFQPPSAPLNVYFELDDAQNEWAYNEPFDFIHMRNLSGAFANWGAVYAEVSKNLKPGGSFEIADPGPIQYKRETSNSNTSIFNGAIRSAADMSGRPLDLDHLKKPMFDNAGLSVAKTRVFEIPLGTYDPDPRKKLTGKMAMIAVLEGLEAVSLRLLTKHLGWKEEDVRGLCRKVQEEVMRPDARAFIRVQFVIARKLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CI81</accession>
    <name>A0A8H6CI81_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010644</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153130.1">
        <property type="nucleotide sequence ID" value="XM_037301496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CI81"/>
    <dbReference type="GeneID" id="59339034"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053025">
        <property type="entry name" value="Mito_ATP_Synthase-Asso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44873">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 30, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44873:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 30, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034554297" description="J domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="37"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="263" mass="28780" checksum="5E22AF7D5DCF3888" modified="2022-01-19" version="1">MPLSRSLLPTAFLLPRLALPRTALFHTTSAWRSESASYYKTLGIDPSASAGDVKTQFYSLSKTHHPDHNANDPQASERFVKISEAYAVLGSPQKRERYDRDIQDAQESSRSHAHRGSHSSSSTPFGSRPGSGLSRRRTQFKGPPPSFYRSGGWGAQGSKRHAQAEGSGSAGTGDPIGGGFGPGRGQAGFNDVPHFNREGHHHTQEQQDQRRRKRLEEESVGFAEGGSVLFQFLLITAVVTFAFSIPVLFDRSRDGAKRTSVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CID5</accession>
    <name>A0A8H6CID5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153220.1">
        <property type="nucleotide sequence ID" value="XM_037301586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CID5"/>
    <dbReference type="GeneID" id="59339124"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034089667" evidence="1">
        <location>
            <begin position="21"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="242" mass="25982" checksum="8CEED045F0A6782D" modified="2022-01-19" version="1">MMPFKFIVAGAMMFFSLALALPEPIPVAVFEAVPAVLPEAAPVAVREAKELVSPLQACTPAGASCKSPAATMTTPSIACIGIIGKHDNPLHIALFPPHISAPSAHLEFSFLLNASLDIFDACARDRNRIDQDLGMLQAIDERLSIWGWETGTGTRFAVVVDAWGRGGNRGLRGVSDGDLRGAFKALQTAYVRLLQNPFYVPDEHTPMAVASGRGKGGEITSKRFIEEVERIGEIWKPGMSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIF9</accession>
    <name>A0A8H6CIF9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153112.1">
        <property type="nucleotide sequence ID" value="XM_037301478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIF9"/>
    <dbReference type="GeneID" id="59339016"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="177" mass="18959" checksum="56D43E44D53299E3" modified="2022-01-19" version="1">MAIPATARSPAATEPTFFVAAPVKLGPDPVFDGETGVIGEPEAAPDPEPEPEPEPEPEPEPEPEPEPEPEPEPEPDPELEPEPAVPVAKGPDAPVPVASPVAPATPLVLSTRVSRRAIMVNGFWCLTQQLWFLCMNILYQSAELLELADGDAVPDADVELEDELEHAKRVAELANDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CIY9</accession>
    <name>A0A8H6CIY9_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153393.1">
        <property type="nucleotide sequence ID" value="XM_037301759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CIY9"/>
    <dbReference type="GeneID" id="59339300"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="404" mass="44242" checksum="4F878B8489CDA960" modified="2022-01-19" version="1">MAYQESSYGPRSTYAGPTNNAPHFPQSHQQHSHSHSQFYPNPYPHSTQSNASSPPLPSYTPPQPSEGQTYHPQRLPYGPNQIHNVGGLSTQPPMTAPDDHFKKPYLPQQQYMNHGQPPANFIDHQRQKSSSAVSELAPAQAYSGASTTPQRLHTSSSSYPPTSTMTVTAAPINPPSTQSTPTSEVPSQSADPALQPGGLSSLDQQRVDALLQVNNLLLQEVTILQKVGFKPSPQSPQQTAPPNTSSPQATPAPPTSAGDPTSTTQNQNNSVTKTNPTVSTPTTTTPTTATAPQQPSLNTKKYVEFMTRIKTNIMFLVSISDQAKDKPRLPYPSHLEAPPLWLNPGNEEGDRQLDLLREEYTRLRELWPDWKPEVRKPQVGGQPVQQGLQQGLQQMQQPQVQQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ10</accession>
    <name>A0A8H6CJ10_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HO133_010914</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">WasteWater1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LovG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153397.1">
        <property type="nucleotide sequence ID" value="XM_037301763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ10"/>
    <dbReference type="GeneID" id="59339304"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF3">
        <property type="entry name" value="ESTERASE DBAE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="4">
        <location>
            <begin position="9"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF6224337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="267" mass="29117" checksum="B32E694F4B19F426" modified="2022-01-19" version="1">MVESDDHLPRLLCLHGGGTSAMIFKIQTRRLQWALRSTFKFVFADGPFPSGAGPGVLPVFAGCEPYFRWLNPASQAPEQDQRRVRQALRKVMDDDGGEFVGVLGFSQGARLTAGLLADQDERNAADGMPNWKFGVMLCGSFPPLSLSLARTPSTKPSQGVDEHGETREPEDDEIVHVPCVLVRGTLDPHSEKGRRLVKYFDPETAISMVFKMGHHLPGAAGDTTSDKGDTQKIVDAILKVYADHLPYEVLKAPKATAIEYGNGQLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CJ56</accession>
    <name>A0A8H6CJ56_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000009">
        <property type="protein sequence ID" value="KAF6224116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153176.1">
        <property type="nucleotide sequence ID" value="XM_037301542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CJ56"/>
    <dbReference type="GeneID" id="59339080"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010640">
        <property type="entry name" value="Low_temperature_requirement_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840">
        <property type="entry name" value="BLL5714 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840:SF1">
        <property type="entry name" value="BLL5714 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="258" mass="29529" checksum="33E96D023A26801E" modified="2022-01-19" version="1">METTSTPPLDNTTPPQASHGHLHTGRRLRKLLRPNGRRVHIAGTPEEHVHLTRTLPNIEPDDNFDCYLQGSTEHLEAVREIHAFHENRSHDLRTQHGSIYDEFELVRSELDTLADELLHLSNHGVALNANFSKFGYDAHIRTKDSDSSTSSLSGDQSSSHRDWEAERRKGQALKFWKKPIVRQYWHRGLLWRASEVEEVASFELFVDLLYVGIISVIGDTAAENATGFNLLKFTITFALAFKMWTDLTLLISWFETSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK19</accession>
    <name>A0A8H6CK19_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_010097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153770.1">
        <property type="nucleotide sequence ID" value="XM_037300953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK19"/>
    <dbReference type="GeneID" id="59338489"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6224903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="442" mass="48420" checksum="E2623B2FF592BFA8" modified="2022-01-19" version="1">MSSVHTVMSGSFSFDTILPPKLDINVGAKSHFFQPPKTPSASSSLHRSTASLPAQDVINKTASRERSRHVSHSSDQLTPFKTLSNASPSMLSFTPSGLESPSSLSPVPFVNTQYRLAGGLDTPTARLTMDEEGGECRASPGLHLRGFRDSQSVPDSYFPQSASALSRESNGRPRQHASPRIQDGFGKAVYGIASVAGKLWDFCRTTAFKGFYSGGGLGYEMKPPLGYMNCDQNMWQEVDCDDVFQSNLCEKSLVPGCFPEEDFIADYMSQIHHTTPPRAAKKVQREKGIGGEISASWVLVGSTSTSRETSPSRLSSRKNPPSNTSARRPIPKLGRRPILPASRPSLTSYAGSPGLHSDRSASFASPRSPITSLKHESPPHAEVHRQAARIRKRELEEDANLKRFNQQLKAMIKEGKEALGTKFEVLEPVDENYAYVYGCRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CK64</accession>
    <name>A0A8H6CK64_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153621.1">
        <property type="nucleotide sequence ID" value="XM_037300804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CK64"/>
    <dbReference type="GeneID" id="59338340"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022085">
        <property type="entry name" value="OpdG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12311">
        <property type="entry name" value="DUF3632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6224754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="199" mass="22458" checksum="83E919E3062EBB19" modified="2022-01-19" version="1">MWDLWNIVCDAIDHLGDPTSNLEQLVQMMICISKLPDVVDVNGEVVKPSMNSKVFWRELPGFAFYFREVATPTDVPPTSQLLRGNPITWGEQVQKHTHANTFKALYLHALDSNLQTPHRHFAAMHNHAQWTLCEALEVPTDHVEQVRRTEMYVPAASQWILKAGATVSRFCAQRANNDGERVVQRWIGGDNSGGVDVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKB8</accession>
    <name>A0A8H6CKB8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HO133_010330</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Binds to F-actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the WD repeat coronin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6225134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154001.1">
        <property type="nucleotide sequence ID" value="XM_037301184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKB8"/>
    <dbReference type="GeneID" id="59338721"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000197">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF0">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08953">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="12">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="77"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="134"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="176"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="415"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="612"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="468"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="483"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="542"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="555"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF6225134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="642" mass="70537" checksum="C10529744B9DBBB0" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKP6</accession>
    <name>A0A8H6CKP6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylglycerol/phosphatidylinositol transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HO133_010075</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">WasteWater1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the intermembrane transfer of phosphatidylglycerol and phosphatidylinositol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000008">
        <property type="protein sequence ID" value="KAF6224881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037153748.1">
        <property type="nucleotide sequence ID" value="XM_037300931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKP6"/>
    <dbReference type="GeneID" id="59338467"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00917">
        <property type="entry name" value="PG-PI_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033917">
        <property type="entry name" value="ML_PG-PI_TP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF0">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL_PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034112301" description="Phosphatidylglycerol/phosphatidylinositol transfer protein" evidence="8">
        <location>
            <begin position="21"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="9">
        <location>
            <begin position="43"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF6224881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="166" mass="18130" checksum="AFEF7EF16FEE420E" modified="2022-01-19" version="1">MKLTTTLLPLLLSSLATSSSLSFFGSNQQVLDDDLKVPGDNPLEFCAKSDDFILTINNVDLEPNPPLPGKTLTIKAKGNFTEEVSQGAYVVLVVKYGLIRLINQQADLCEQMKSAVDEECPLIGEKTITKDVDLPKEIPPGHYTVSADVYTEDHVKVTCLTASIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CKT4</accession>
    <name>A0A8H6CKT4_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_009465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154174.1">
        <property type="nucleotide sequence ID" value="XM_037300326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CKT4"/>
    <dbReference type="GeneID" id="59337860"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6225465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="350" mass="40516" checksum="7C0DB89ADAC29684" modified="2022-01-19" version="1">MALTWTRFVPDHYDSAAQSPPSPADTVVAHPFPFMRLPVEIRLQAYREYLLDRYSPSPAEIHEMVLDTCDWEKPPAEILQVSKAVNAEVQDLLRHETAFNLRICWQDATFDGLAVSCFRARGKPLDYDGIAHLRIEIYPAHPDRYTDMIRVWRQVQKVCSDLQGASCVQNLSLHFMENQYATWSLDGEPRGTMDMSCNAKRLPSDVLHILDLFKLLANVAKAQIHLPVSLNEDVSLQESKRDTEDVMMQIKSLDHAHQKLVIKTIEEAIAENEEDLKYVTSSNSHDKLDRLCGYGHWISNPHFDIFEKVWPHRDSVFEWEYRPRSQYIGDESLENAPLDYVDPYDSDDYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CLS5</accession>
    <name>A0A8H6CLS5_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Centromere protein H C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009859</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CENP-H/MCM16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154566.1">
        <property type="nucleotide sequence ID" value="XM_037300718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CLS5"/>
    <dbReference type="GeneID" id="59338254"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040034">
        <property type="entry name" value="CENP-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008426">
        <property type="entry name" value="CENP-H_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48122">
        <property type="entry name" value="CENTROMERE PROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48122:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05837">
        <property type="entry name" value="CENP-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centromere protein H C-terminal" evidence="8">
        <location>
            <begin position="29"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6225857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="239" mass="26561" checksum="255501DA27758445" modified="2022-01-19" version="1">MSSSTDIAFADLASHRSGDAIPFYETEGQILELWDQLNELRLEQALLEAETTVPLMQQPLTDEEMDSQVTIAEKECLEARATYLLKQSVVEDVLIVDPVLKAVHSGLNATPTERALHPLIDRRDTLEIAHTNLSSTLQTLLKEAAMLSADNIRAMEKNRALTATLLVLAEKVQAQRDEIIMDPRFSAQLDGLRIDAATARQRWRIMKSVVAAVIAGSGVDWARDDTLRDLVLDDENEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CM64</accession>
    <name>A0A8H6CM64_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">nitric-oxide synthase (NADPH)</fullName>
            <ecNumber evidence="3">1.14.13.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_009676</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.39" evidence="3"/>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154385.1">
        <property type="nucleotide sequence ID" value="XM_037300537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CM64"/>
    <dbReference type="GeneID" id="59338071"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004517">
        <property type="term" value="F:nitric-oxide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006809">
        <property type="term" value="P:nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.340.10">
        <property type="entry name" value="Nitric Oxide Synthase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1230.10">
        <property type="entry name" value="Nitric Oxide Synthase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.440.10">
        <property type="entry name" value="Nitric Oxide Synthase,Heme Domain,Chain A domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050607">
        <property type="entry name" value="NOS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044943">
        <property type="entry name" value="NOS_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044940">
        <property type="entry name" value="NOS_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044944">
        <property type="entry name" value="NOS_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004030">
        <property type="entry name" value="NOS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036119">
        <property type="entry name" value="NOS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43410:SF1">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43410">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02898">
        <property type="entry name" value="NO_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56512">
        <property type="entry name" value="Nitric oxide (NO) synthase oxygenase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="11">
        <location>
            <begin position="557"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6225676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="1121" mass="125667" checksum="3CDA9FFB72CE4175" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CME1</accession>
    <name>A0A8H6CME1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger Mcm10/DnaG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HO133_009798</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000007">
        <property type="protein sequence ID" value="KAF6225796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154505.1">
        <property type="nucleotide sequence ID" value="XM_037300657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CME1"/>
    <dbReference type="GeneID" id="59338193"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040184">
        <property type="entry name" value="Mcm10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055065">
        <property type="entry name" value="OB_MCM10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015408">
        <property type="entry name" value="Znf_Mcm10/DnaG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13454">
        <property type="entry name" value="PROTEIN MCM10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13454:SF11">
        <property type="entry name" value="PROTEIN MCM10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22379">
        <property type="entry name" value="OB_MCM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09329">
        <property type="entry name" value="zf-primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MCM10 OB-fold" evidence="10">
        <location>
            <begin position="469"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger Mcm10/DnaG-type" evidence="9">
        <location>
            <begin position="544"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="88"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="595"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="751"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="611"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="763"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="782"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF6225796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="798" mass="87115" checksum="11A922F507F9F585" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMG5</accession>
    <name>A0A8H6CMG5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154785.1">
        <property type="nucleotide sequence ID" value="XM_037299959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMG5"/>
    <dbReference type="GeneID" id="59337493"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="90" mass="9788" checksum="21DA745D5BB4AB5C" modified="2022-01-19" version="1">MAGFDGTDYGCDDDDSPTTDATGPNARERGIGGQETVPVDGDGAVQELDAGFSDDESDDDEGEEVVLLRMSRTVMRGERFRRRLCLKEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMH7</accession>
    <name>A0A8H6CMH7_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HO133_009014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154701.1">
        <property type="nucleotide sequence ID" value="XM_037299875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMH7"/>
    <dbReference type="GeneID" id="59337409"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF6226148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="642" mass="67597" checksum="99A1941FFD3153D1" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMI4</accession>
    <name>A0A8H6CMI4_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008988</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154675.1">
        <property type="nucleotide sequence ID" value="XM_037299849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMI4"/>
    <dbReference type="GeneID" id="59337383"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10004">
        <property type="entry name" value="RPD3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="11">
        <location>
            <begin position="39"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="389"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="436"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="481"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="582"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="597"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6226122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="626" mass="69538" checksum="9638861B4470CA7D" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CMQ0</accession>
    <name>A0A8H6CMQ0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HO133_009108</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WasteWater1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000006">
        <property type="protein sequence ID" value="KAF6226242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037154795.1">
        <property type="nucleotide sequence ID" value="XM_037299969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CMQ0"/>
    <dbReference type="GeneID" id="59337503"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18870">
        <property type="entry name" value="NUDIX_AcylCoAdiphos_Nudt19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039121">
        <property type="entry name" value="NUDT19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12318:SF0">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE NUDT19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12318">
        <property type="entry name" value="TESTOSTERONE-REGULATED PROTEIN RP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="8">
        <location>
            <begin position="39"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF6226242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="366" mass="40716" checksum="A9C9255CF6C091BA" modified="2022-01-19" version="1">MFRRRKDLDFFEAAMRGASPKKQSSRRSSSSKSMKELPPANPSASVLLISAANHILLLHRVSSSSTFASAHVFPGGHCSPQDGHLPPPSDPSHHQDSPAYRHAAIRECFEESGILLARKQENTAELVELAEQEREEGRKAVHAEKVPFADWIKQKGGRMDTEGLVPFTRWLTPANIPKRYSTQMYLYFLPLESGPRLGKQTDQMHIPTPDGGVEHTAAKFAWAGEWIEAALKGECVLFPPQFFLLSLVADFLKPPLAGERELDTEQLQKQRGQLMEFVKGEDPPWGEKCISPNPIKKSGDTLWMGMGDAGPELEGTGRGGDKERVLRVELSGEVERGRRTPRPKEVVMRKDLGAGKGEGKGHLWRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CNY8</accession>
    <name>A0A8H6CNY8_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
            <ecNumber evidence="2">2.7.1.150</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Type III PIP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HO133_008404</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">WasteWater1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.150"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.150" evidence="2"/>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6226963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155271.1">
        <property type="nucleotide sequence ID" value="XM_037299270.1"/>
    </dbReference>
    <dbReference type="GeneID" id="59336800"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03334">
        <property type="entry name" value="Fab1_TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17300">
        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000007">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000283">
        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase (Fab1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000005">
        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase (Fab1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044769">
        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF7">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="14">
        <location>
            <begin position="502"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="15">
        <location>
            <begin position="2179"/>
            <end position="2509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="139"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="274"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="415"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="564"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="787"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1150"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1702"/>
            <end position="1722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1745"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1807"/>
            <end position="1841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1851"/>
            <end position="1870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1877"/>
            <end position="1921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2021"/>
            <end position="2102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
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        <location>
            <begin position="278"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="423"/>
            <end position="433"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
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            <begin position="460"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="598"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
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        <location>
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            <end position="1213"/>
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        <location>
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        <location>
            <begin position="1894"/>
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        <location>
            <begin position="2043"/>
            <end position="2061"/>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00012009"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00075294"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF6226963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="2545" mass="282308" checksum="60E1CFE48C87DEA6" modified="2022-01-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CP75</accession>
    <name>A0A8H6CP75_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008753</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_037299617.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="proteasome_alpha_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Probable proteasome subunit alpha type-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
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        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="5">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="245"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="277"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF6227310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="293" mass="30929" checksum="473D0C4A9337447F" modified="2022-01-19" version="1">MTSIGTGYDLQNSVFSPDGRNFQVEYAVKAVENGGTCVGIRCKDGVVLAVEKIVTSKLLKPGANKRIATVDRNVGVVSSGLVPDGRHFVSRARDEASSWRSTYKAPIPLAALAGRLGGYVQAYTLYSSVRPFGVTAIVGGWDSEAELGVDGQVGSGPSIGAGGKVSGTKAGGPGLYMIEPSGLYWGYYGAATGKGRQAAKAELEKLDLASGNLSLEAGVREAARIIYVAHEDSKDKDFELEMSWISSTEGPTKGRHQEVPKDILEEAEKAAKKAMEGDDEEETTAAEGETMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPQ1</accession>
    <name>A0A8H6CPQ1_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nascent polypeptide-associated complex subunit alpha-like UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HO133_008507</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155374.1">
        <property type="nucleotide sequence ID" value="XM_037299373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPQ1"/>
    <dbReference type="GeneID" id="59336903"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14361">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052617">
        <property type="entry name" value="Huntingtin-int_K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038922">
        <property type="entry name" value="HYPK_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31184:SF2">
        <property type="entry name" value="HUNTINGTIN-INTERACTING PROTEIN K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31184">
        <property type="entry name" value="HUNTINGTIN-INTERACTING PROTEIN K FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nascent polypeptide-associated complex subunit alpha-like UBA" evidence="2">
        <location>
            <begin position="95"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF6227066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="169" mass="18293" checksum="65878231B9730CFA" modified="2022-01-19" version="1">MAEPQPSSVHEGMDREDPAPLPTSAEDRKAAAAMSSLERRGGDDDDAATKPNKEIDQEALGKAISRLELADKAGTVAAGEKKTKEKEEVEKRAKIKVDQADVTLLVEELDLTKGKATELLKANDGDAVKAMRGFVMLRIHNLTLDRMLITSKEVNGESEEVPQAQTEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPV5</accession>
    <name>A0A8H6CPV5_9LECA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HO133_008854</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155718.1">
        <property type="nucleotide sequence ID" value="XM_037299717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPV5"/>
    <dbReference type="GeneID" id="59337249"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF6227410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="437" mass="49126" checksum="1A9628DC731E19BA" modified="2022-01-19" version="1">MAQVCSLSDIRSLKKIRLVNTIFAQVAAQYLYFDKSTEVAFHPTLRFHVRTLYFDRDILDEIFAEYETWEATIDTRELATTGDTRAKNPEEARWQCSQADLDRSHTNFCRLLASQRALFHGRMDLIVLSAALPMLQNLRTIESIEKAFRNGAVATPYDSIAGHEKDWVPILSDLQTETLLPTPFIDLSLSTQPGTARPLAPLISGLGLARRQLRTKELRRIPRSFWKQEGPSGFEHGVRSHIHAAFRYLESLDVQFVVGFYDLEVSLQGLMPPSANNFIGAAPGLRLLDLTFLCYEELDDGAGFGDANWGTLPRTGQEISMSSARLDNRSVGSTSWEETLKQIAPILFLDWVKLRWLWSDDIEDVIGAGDPNDEAFSSRHAAYCQSLTDFLCNREAGRNVLGSSTSRDRLGESLKMGGVGKLDIRAIHRRNSVRPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CPY0</accession>
    <name>A0A8H6CPY0_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Quinate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HO133_008527</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">WasteWater1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155394.1">
        <property type="nucleotide sequence ID" value="XM_037299393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CPY0"/>
    <dbReference type="GeneID" id="59336923"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF34">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0672">Quinate metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF6227086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000593566"/>
        </source>
    </evidence>
    <sequence length="510" mass="56244" checksum="0359204F3ADE23B8" modified="2022-01-19" version="1">MGAAMFGYDSAFIGGALTLPSFQKRFGLESAHGTKLANLKANIVSTFQAGAFFGAILVYPVTEKFGRRLTLLLCGLLFNLGAIAQLASSGHVGAIYAGRVLTGLAVGASSLIIPVYISESSPPAIRGRLVGLFECFLQVSQVIGFWMNYGVSLHVPKTLDKQWQIPFSFQVVPASLLVILMFFQPESPRWLVKAGKADQARRNLSRLRQLPLDHEYISWEINTAQRQIEAENSLGANRSVWAKVQQAFTLPGNRQRLLIGMALMLLQNMSGINALNYYSPAIFAAIGFSGTDVSLLATGVFGLVKATTTLILMLFLIDRLGRRRAMLIGSCGGIVAMYYLGGYTAQSHSFTSEHVPKDGGAYIAILMVYLFAFFYALSWNGVPWVFCSEVFPTAIRSVCLVFTTCSQWLAQFIIVYSTPYMMTNITYGTFLLFGTSLVVGIVFIYLFFPETKGLLLEEMDILFSQRGPATAKRRETDRIIQDRRNDGHAQAVVAEKRDEKTDPMIHVEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8H6CQB6</accession>
    <name>A0A8H6CQB6_9LECA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HO133_008739</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Letharia lupina</name>
        <dbReference type="NCBI Taxonomy" id="560253"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Lecanoromycetes</taxon>
            <taxon>OSLEUM clade</taxon>
            <taxon>Lecanoromycetidae</taxon>
            <taxon>Lecanorales</taxon>
            <taxon>Lecanorineae</taxon>
            <taxon>Parmeliaceae</taxon>
            <taxon>Letharia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Genomics" volume="112" first="3150" last="3156">
            <title>Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture.</title>
            <authorList>
                <person name="McKenzie S.K."/>
                <person name="Walston R.F."/>
                <person name="Allen J.L."/>
            </authorList>
            <dbReference type="PubMed" id="32504651"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">WasteWater1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JACCJB010000005">
        <property type="protein sequence ID" value="KAF6227296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037155604.1">
        <property type="nucleotide sequence ID" value="XM_037299603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8H6CQB6"/>
    <dbReference type="GeneID" id="59337134"/>
    <dbReference type="Proteomes" id="UP000593566">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051965">
        <property type="entry name" value="ChromReg_NeuronalGeneExpr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040:SF3">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
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